LOCUS       DACCXM010000001        74063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCXM010000001 DACCXM010000000
VERSION     DACCXM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.001"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(90..1649)
                     /locus_tag="IVY61_000001"
     CDS             complement(90..1649)
                     /locus_tag="IVY61_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP7969147.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1804..4038
                     /gene="pcrA"
                     /locus_tag="IVY61_000002"
     CDS             1804..4038
                     /gene="pcrA"
                     /locus_tag="IVY61_000002"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP7969148.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4055..6091
                     /gene="ligA"
                     /locus_tag="IVY61_000003"
     CDS             4055..6091
                     /gene="ligA"
                     /locus_tag="IVY61_000003"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP7969149.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6197..6502
                     /gene="gatC"
                     /locus_tag="IVY61_000004"
     CDS             6197..6502
                     /gene="gatC"
                     /locus_tag="IVY61_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP7969150.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6502..7974
                     /gene="gatA"
                     /locus_tag="IVY61_000005"
     CDS             6502..7974
                     /gene="gatA"
                     /locus_tag="IVY61_000005"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP7969151.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7971..9401
                     /gene="gatB"
                     /locus_tag="IVY61_000006"
     CDS             7971..9401
                     /gene="gatB"
                     /locus_tag="IVY61_000006"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP7969152.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9411..10466
                     /locus_tag="IVY61_000007"
     CDS             9411..10466
                     /locus_tag="IVY61_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7969153.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10577..11950
                     /gene="rlmD"
                     /locus_tag="IVY61_000008"
     CDS             10577..11950
                     /gene="rlmD"
                     /locus_tag="IVY61_000008"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7969154.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11953..12081
                     /locus_tag="IVY61_000009"
     CDS             11953..12081
                     /locus_tag="IVY61_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7969155.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12521..12709
                     /locus_tag="IVY61_000010"
     CDS             12521..12709
                     /locus_tag="IVY61_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP7969156.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12787..13800
                     /locus_tag="IVY61_000011"
     CDS             12787..13800
                     /locus_tag="IVY61_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7969157.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13867..14901)
                     /locus_tag="IVY61_000012"
     CDS             complement(13867..14901)
                     /locus_tag="IVY61_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7969158.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15016..17241
                     /locus_tag="IVY61_000013"
     CDS             15016..17241
                     /locus_tag="IVY61_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7969159.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17259..19142
                     /locus_tag="IVY61_000014"
     CDS             17259..19142
                     /locus_tag="IVY61_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP7969160.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19123..20097
                     /locus_tag="IVY61_000015"
     CDS             19123..20097
                     /locus_tag="IVY61_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP7969161.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            20756..21778
                     /gene="aroF"
                     /locus_tag="IVY61_000016"
     CDS             20756..21778
                     /gene="aroF"
                     /locus_tag="IVY61_000016"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP7969162.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            21811..22875
                     /gene="aroB"
                     /locus_tag="IVY61_000017"
     CDS             21811..22875
                     /gene="aroB"
                     /locus_tag="IVY61_000017"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP7969163.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            22877..24043
                     /gene="aroC"
                     /locus_tag="IVY61_000018"
     CDS             22877..24043
                     /gene="aroC"
                     /locus_tag="IVY61_000018"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP7969164.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            24060..25151
                     /locus_tag="IVY61_000019"
     CDS             24060..25151
                     /locus_tag="IVY61_000019"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP7969165.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            25164..26459
                     /gene="aroA"
                     /locus_tag="IVY61_000020"
     CDS             25164..26459
                     /gene="aroA"
                     /locus_tag="IVY61_000020"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP7969166.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            26452..26961
                     /locus_tag="IVY61_000021"
     CDS             26452..26961
                     /locus_tag="IVY61_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP7969167.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            26958..27794
                     /gene="pheA"
                     /locus_tag="IVY61_000022"
     CDS             26958..27794
                     /gene="pheA"
                     /locus_tag="IVY61_000022"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP7969168.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(27857..29593)
                     /locus_tag="IVY61_000023"
     CDS             complement(27857..29593)
                     /locus_tag="IVY61_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7969169.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(29590..30519)
                     /locus_tag="IVY61_000024"
     CDS             complement(29590..30519)
                     /locus_tag="IVY61_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7969170.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            complement(30519..31097)
                     /locus_tag="IVY61_000025"
     CDS             complement(30519..31097)
                     /locus_tag="IVY61_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7969171.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            31370..32656
                     /locus_tag="IVY61_000026"
     CDS             31370..32656
                     /locus_tag="IVY61_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7969172.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            32793..33662
                     /locus_tag="IVY61_000027"
     CDS             32793..33662
                     /locus_tag="IVY61_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7969173.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            33659..34546
                     /locus_tag="IVY61_000028"
     CDS             33659..34546
                     /locus_tag="IVY61_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7969174.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            34713..34787
                     /locus_tag="IVY61_000029"
                     /pseudo
     CDS             34713..34787
                     /locus_tag="IVY61_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
     gene            34941..35345
                     /locus_tag="IVY61_000030"
     CDS             34941..35345
                     /locus_tag="IVY61_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP7969175.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            35362..36510
                     /gene="tnpB"
                     /locus_tag="IVY61_000031"
     CDS             35362..36510
                     /gene="tnpB"
                     /locus_tag="IVY61_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP7969176.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            36676..37287
                     /locus_tag="IVY61_000032"
                     /pseudo
     CDS             36676..37287
                     /locus_tag="IVY61_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
     gene            37284..38057
                     /locus_tag="IVY61_000033"
     CDS             37284..38057
                     /locus_tag="IVY61_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7969177.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            38054..38452
                     /locus_tag="IVY61_000034"
     CDS             38054..38452
                     /locus_tag="IVY61_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP7969178.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            38637..39308
                     /locus_tag="IVY61_000035"
     CDS             38637..39308
                     /locus_tag="IVY61_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969179.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            39367..39807
                     /locus_tag="IVY61_000036"
     CDS             39367..39807
                     /locus_tag="IVY61_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969180.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            39819..40487
                     /locus_tag="IVY61_000037"
     CDS             39819..40487
                     /locus_tag="IVY61_000037"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7969181.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            40551..41174
                     /locus_tag="IVY61_000038"
     CDS             40551..41174
                     /locus_tag="IVY61_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7969182.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            41230..41616
                     /locus_tag="IVY61_000039"
     CDS             41230..41616
                     /locus_tag="IVY61_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP7969183.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            41829..43220
                     /locus_tag="IVY61_000040"
     CDS             41829..43220
                     /locus_tag="IVY61_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP7969184.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            43831..44199
                     /locus_tag="IVY61_000041"
     CDS             43831..44199
                     /locus_tag="IVY61_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7969185.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            44379..45320
                     /locus_tag="IVY61_000042"
     CDS             44379..45320
                     /locus_tag="IVY61_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP7969186.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            45389..46120
                     /locus_tag="IVY61_000043"
     CDS             45389..46120
                     /locus_tag="IVY61_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP7969187.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            46264..48444
                     /locus_tag="IVY61_000044"
     CDS             46264..48444
                     /locus_tag="IVY61_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP7969188.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            48464..48946
                     /locus_tag="IVY61_000045"
     CDS             48464..48946
                     /locus_tag="IVY61_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP7969189.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            48962..49723
                     /locus_tag="IVY61_000046"
     CDS             48962..49723
                     /locus_tag="IVY61_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7969190.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            49854..51188
                     /locus_tag="IVY61_000047"
     CDS             49854..51188
                     /locus_tag="IVY61_000047"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP7969191.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            51495..51980
                     /locus_tag="IVY61_000048"
     CDS             51495..51980
                     /locus_tag="IVY61_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP7969192.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            51973..52656
                     /locus_tag="IVY61_000049"
     CDS             51973..52656
                     /locus_tag="IVY61_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP7969193.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            52782..55427
                     /gene="polA"
                     /locus_tag="IVY61_000050"
     CDS             52782..55427
                     /gene="polA"
                     /locus_tag="IVY61_000050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP7969194.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            55472..56308
                     /gene="mutM"
                     /locus_tag="IVY61_000051"
     CDS             55472..56308
                     /gene="mutM"
                     /locus_tag="IVY61_000051"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP7969195.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            56272..56901
                     /locus_tag="IVY61_000052"
     CDS             56272..56901
                     /locus_tag="IVY61_000052"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP7969196.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            56953..57285
                     /locus_tag="IVY61_000053"
     CDS             56953..57285
                     /locus_tag="IVY61_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7969197.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            57597..58088
                     /gene="nrdR"
                     /locus_tag="IVY61_000054"
     CDS             57597..58088
                     /gene="nrdR"
                     /locus_tag="IVY61_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP7969198.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            58111..59484
                     /locus_tag="IVY61_000055"
     CDS             58111..59484
                     /locus_tag="IVY61_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP7969199.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            59484..60410
                     /gene="dnaI"
                     /locus_tag="IVY61_000056"
     CDS             59484..60410
                     /gene="dnaI"
                     /locus_tag="IVY61_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP7969200.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            60539..60979
                     /locus_tag="IVY61_000057"
     CDS             60539..60979
                     /locus_tag="IVY61_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7969201.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            61123..61824
                     /locus_tag="IVY61_000058"
     CDS             61123..61824
                     /locus_tag="IVY61_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP7969202.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            61821..62942
                     /locus_tag="IVY61_000059"
     CDS             61821..62942
                     /locus_tag="IVY61_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP7969203.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            62957..64189
                     /gene="pepT"
                     /locus_tag="IVY61_000060"
     CDS             62957..64189
                     /gene="pepT"
                     /locus_tag="IVY61_000060"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP7969204.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            64321..65229
                     /locus_tag="IVY61_000061"
     CDS             64321..65229
                     /locus_tag="IVY61_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP7969205.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            65264..65536
                     /locus_tag="IVY61_000062"
     CDS             65264..65536
                     /locus_tag="IVY61_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP7969206.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            65547..66593
                     /locus_tag="IVY61_000063"
     CDS             65547..66593
                     /locus_tag="IVY61_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP7969207.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            66844..69453
                     /gene="clpB"
                     /locus_tag="IVY61_000064"
     CDS             66844..69453
                     /gene="clpB"
                     /locus_tag="IVY61_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP7969208.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(69604..70275)
                     /locus_tag="IVY61_000065"
     CDS             complement(69604..70275)
                     /locus_tag="IVY61_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969209.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(70268..70762)
                     /locus_tag="IVY61_000066"
     CDS             complement(70268..70762)
                     /locus_tag="IVY61_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7969210.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(70782..72002)
                     /locus_tag="IVY61_000067"
     CDS             complement(70782..72002)
                     /locus_tag="IVY61_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7969211.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            72149..73984
                     /gene="lepA"
                     /locus_tag="IVY61_000068"
     CDS             72149..73984
                     /gene="lepA"
                     /locus_tag="IVY61_000068"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP7969212.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        24530 a        13192 c        15938 g        20403 t
ORIGIN      
        1 gttttcccag cctcttcttt ttttatagaa tcatatagaa tcaagtagtg tagatgaata
       61 gttgttgtaa aatagttttt ccatgttcgt taaaatgatt gcttcaaatt tgtgatagaa
      121 gatttagcct cgatttcctg cttgattctt ggcaagtttg cttcatcagt caagataacc
      181 aaaatatctc ctacattcat gatcgtatca ccgtgagtca agatttcctg atttcctctg
      241 cgtatagaga ttagcagcat atttttaggc caattgaagt ctcgtaccat taatccgtct
      301 aaagaactct ctgccgttac tggaaaatca aaagttgtcc ttgcaccttt tagtgaggcg
      361 gtctgagtat ttccaagtag acgttccaat aatgattcgt aaattggatt accgccgaga
      421 aggtcattga cgatataagc aacaagagca gccaatccta aaggcatcag atgattaaga
      481 gagccaacca tttctgtcac taaaataatt gctgtcagcg gtgcttttcc aatcgctgtg
      541 aaataaccag ccatggcaat aacgacaaaa tccttgatgt agattggatc catcccgaaa
      601 tcaaccataa tgtttccata aaagctaccg attaaagccc ccaatgtcaa tatcggaagg
      661 aaaatccccc ctggaagacc cccaccgtaa gaaatcatgg aaaaaacgaa tcgaagaaca
      721 aacaaaccga taatcataat gattggcaag gaagcatgtc caaaagctaa aacgatttga
      781 ttgcctccgc ctagagtact aggccataaa taaccaattg gcaaaatcag caagaaagga
      841 acgaacccgt gaaaataagc tggtaaaggc aatcggttgt aaaattttgg caaagccagc
      901 aagacattct gataaataaa acctaagatg cctagaagta ctcctaagat cactaaggaa
      961 ccataatatt tcaacggcaa agaaggaagg tcacctagat ataaaacagg tctcaagccg
     1021 aaaaagtaaa gagagacgaa gttagcagtg attgctgaag caaatgatgt cagccaaaca
     1081 agtggagaaa aggagtgatg gatttcttct aaaacaaaga gtaatcctgc gataggagca
     1141 ttgaatgctg ctgcaagtcc gctacttgca ccgctagaaa ttaaaatctt ttcttctgaa
     1201 gatggactcc gaaacaattg tccagctcct tgtccaatgg atgcgccaag ctgtatagaa
     1261 ggtccttctc tacctagaaa aaggcccgca ccaactgaaa gtacaccgcc aatgaacttt
     1321 ttccataaaa cagagaacca gttcattgat aattccccgc gaagctgtcc ctctacttgt
     1381 ggaatcccgc ttcccttgat atccggttca tttttgacca gataacccac gaaaaatgct
     1441 gcaaggatgc agcaaatagc taggccaatt aagtaaatcg gctgttcccg actcatttca
     1501 tagatacttc gaactaaatc gcttagcttt tcgatagata atcgaaacag gctaacgacg
     1561 attcctgtca gcagtccaac aaatattcct tttagaataa aatatatctg agtcttatct
     1621 aatttcttga tttttccttt tttttccatt caaacgcctc gctttctttt aatttagcgc
     1681 gaaatcaacg aattgacaag gaaaaatcca taaaaagaaa caaatgattt tgaactagct
     1741 agtttaatga aagccaagaa aatgctataa ttaacaagat tgataaaaga aaggatgttg
     1801 ttaatgtcat ccaaagctga attattaaac gggatgaacc cccgtcagaa agaagctgtt
     1861 cttcacactg acggaccatt actgctaatg gcaggtgcag gaagtggaaa gacgagagtg
     1921 ctgacacacc gcattgccta tttgattgag gaaaaagaag tcaatccttg gaacatccta
     1981 gcgattactt ttaccaataa ggctgcaaaa gaaatgaaag aacgtgtcaa tgcgattctc
     2041 gcatctggag gcgaagatgt ctgggtatcg acttttcact cgatgtgtgt gcgtatttta
     2101 agaagagatg ttgattttat cgggtataac cggaacttta caatcatcga ttcttctgaa
     2161 cagctgacct tgatgaaacg gatactaaaa gaactgaaca ttgatccgaa aaaatatgat
     2221 cctcgaagta tattaggaac aatctctcaa gcaaaaaaca gcctgcagac ccctcaggat
     2281 tttgcaaaaa tgcaaggcag ttattacgaa gagattgcgg caaaatgtta tgctgcttac
     2341 caaaaagagc ttcaatataa tcaatgtatg gactttgacg acctgatcat gaatacgatt
     2401 cgattatttg aagaacatcc tgattcactc acgtattatc aaaacaaatt ccattatatt
     2461 catgtagatg agtaccaaga tacaaatcac gcacagtata cgctagtcaa tttattggca
     2521 ggtagattta ggaacctgtg cgtcgtggga gatgcagatc aaagtattta cggctggcgc
     2581 ggtgcagata tgcagaacat cttggatttt gaaaaagatt atccagatgc tgcagtaatt
     2641 ttgctagaac aaaactatcg ctccacgaaa aacatcctta gcgcagcaaa ccaagtcatt
     2701 gaaaacaata gcaatcgaaa accaaaaaac ttgtggacag aaaacaaaga aggaaacaag
     2761 atcacttact accgtgcaga taatgaacga gacgaaaccc gttttatcgt agaccggatg
     2821 caagaagaga ttcgaagcaa tcatcggaat tacggcgact ttgccatctt gtaccggacg
     2881 aacgcacaat ctcgtgtaat ggaagaaact ctgttgaaag caaatattcc ttataaaatg
     2941 gttggagggc acaagttcta cgatcgtaaa gaaatcaaag atattttagc gtatttgaat
     3001 gtgctggtaa atcctcaaga ttcaatcagt tttgagcgta tcgttaattc gcccaaacga
     3061 ggcatcggac caggatctat cgaaaaacta cgcagttttg cttctcttca tgaatggcca
     3121 ctgcttgaag cagcgcaaaa tgtggatctg gcaaatatcg gtggaaaagc aggacagcaa
     3181 ttaggtgcat ttggagagat gatccaagaa gtcactcaga tgatcccata cttgacagtg
     3241 acagaattga cgaaagaggt tttggatcgt agcggatatc tagaagactt aaaaatccaa
     3301 aatacattag aagcacaagc acgcatcgaa aacttggagg aatttttaac ggtcacacaa
     3361 gaattcgaca aacaattcga acagcaaaat gaagaagacg ctgatgcacc agaagaaaaa
     3421 ctaacggtct tcttaaatga tctggcactt gtttccgaca ttgacaattt agaagaagat
     3481 gcttcccaag tgacattgat gactttgcat gcagccaaag gattagaatt tccagttgta
     3541 ttcctgattg gtctagaaga gggggttttt cctttatctc gagcattgat ggaagaaagc
     3601 gaactggagg aagaacgccg tctcgcatat gtggggatca cgcgagcaga agaagcgttg
     3661 tatttaacaa atgctttctc cagaacatta tatggacgga cgcaatataa ccgaccttct
     3721 cgttttgtag aagaaatcga tcaggagtta ctggaaatcg aaggcatgcg tcctacgcct
     3781 aaaaaaacgc ctgtattcgc taaaaaaaca gcatatagct ataaacagcc agagacagcg
     3841 gttgttccct ctaaaagtgc tactggaggg gaaaataaca gctggaaacc tggagataaa
     3901 gtcaaacaca aaaaatgggg tctaggaaca gtcgtccgag taagcgggac gtcgaaggat
     3961 ctagaactag atgtggcttt cccaagtcaa ggagtaaaac gtcttttggc tgcctttgcg
     4021 ccaattgaaa aagcttagta gtggagggta aatgatggaa catgaaatga cgattgacga
     4081 agctgctaaa cgagcagaag aacttcgtac aagactgaac caatggtctc gcgaatacta
     4141 cgtagaagat aaaccaacag tggaagacta tgtttatgat aaagaatacg cagaattagt
     4201 agcaatcgag gaacaatatc ctgatttgat cacgtctgat tctcccactc aacgagtagg
     4261 cggaaaagta ttagaaggtt ttgaaaaagt cactcatgat attccgcttt acagtttgaa
     4321 cgacgttttc agcaaagaag agctgatagc ttttgatcaa cgtgtacaaa aagcagtggg
     4381 acgtgtagtt gactattgct gtgaactaaa aatagatgga ctttccgttt ctttgcgtta
     4441 tgaagatgga aatttcgtga gaggtgcgac tcgcggggat ggaactgtag gagaaaacat
     4501 cactgaaaac ctaaaaactg tccggtcggt cccaatcaaa ttaaaagaac caatgaacat
     4561 cgaagtaaga ggcgaatgtt tcatgccaaa acggtcattt gtccaattga accaagacag
     4621 agaagcagag ggaaaagata tctttgcgaa tcctcggaac gccgcagctg gttctttgcg
     4681 tcagttagat tctaaaatca cagcgaaaag aaatctggac acttttttat acacagtagc
     4741 tgatttcggt cctatgcaag caaaaaccca gtatgatgcg ctagaagagc tagaaaaaat
     4801 tggattccac acgaatcggg aaaaacgtct atgtcattcg attgatgaag tgtggtcata
     4861 cattgaagaa tatcatgaca aacgtgtgga tttgccctat gaaatcgatg gaatcgtcat
     4921 caaagtgaat gaattctcgc tacaagatca acttggtttt actatcaaag caccgagatg
     4981 ggcagcagct tacaagtttc cgccagaaga agttgaaacg ctaatcgaaa acatcgaatg
     5041 gacagtcggt cgaacagggg tagtaacgcc aacagctatc atgacaccag tccgagtagc
     5101 aggaacgaca gtaagccgtg ccagcctgca taacggtgat tatattaagt tgaaggatat
     5161 ccgcttgaaa gatacagttc tgatttataa agcgggagac atcatcccag aagtttcgca
     5221 agtcgtgtta gataaacgac ctaaagacag cgaagaatac caattgccta cacattgtcc
     5281 agtttgtgga agtgaacttg tccatttgga tgaagaagta gccttacggt gcatcaatcc
     5341 taaatgccct gctcaaatga aagaaggttt gaaccacttt gtttcaagaa atgcgatgaa
     5401 tatcgatggg ttggggccgc gcgtactaga acagatgtat gataaaaaac tagtagcaga
     5461 tgttgctgat ctttacaaat tgacagaaga agagttattg acgttggaca aaataaaaga
     5521 aaaatcagca aataatattt tgacagcaat cgacaacagc aaagataatt cggtcgagcg
     5581 gttgattttc gggttaggaa tccgacatgt cggagcgaaa gcagcaaaaa ttttagcaga
     5641 acatttcggt gatctagaaa cattgagtaa aagtgactat gagtcgatta ttgcgcttga
     5701 tacgatcggt gatatcattg ccgacagtgt agtcacttat ttcagtaacg aagaagtcca
     5761 tgaattgatg aatgaattaa aacaagcagg cgtcaatttc gaatacaaag ggcttcgaaa
     5821 tgcccaattg caagaagtcg agtctccttt taaagaaaaa acagtagtcc ttactggtaa
     5881 actaacacga tttactcgtg aagaagcaaa agagacgatc gaaaatcttg gcgggaaagt
     5941 gactggtagc gtatcgaaaa aaacggatat cgtggtagca ggagaagatg ctggcagcaa
     6001 gctgaccaaa gcacaagaat taggaatcga agtttggaca gaagatcaaa tggcagatgc
     6061 attggctaaa agtcgctcag tagaagaatg aaaaatgagt tttttcagac aaatttcaat
     6121 aaacaatttc agacacggca ctcttgtgtt aaaatagaga gcaaagtttt taactcgttt
     6181 agaaagaagg ggtagtatgg cgattagtga agaacaagtg aaacacgttg ccaaattagc
     6241 aaaacttgcg ttcgcagatg aagaacttgc aagttttacc gaccaattag gtaaaatcat
     6301 cgatatggtt gaaatgctgg aagaagtaga tacggaaggt gttccattta cgtcgaatgt
     6361 ggttgaaaca gtaaacgtga tgcgacaaga tcgggcgcaa gctggatgga accgcgatga
     6421 attattgaaa aatgtgccgg aaacagaaga cggctttatc aaagtacccg caatcatcga
     6481 taatggggag gctggcgcat aatgacagaa ttacacaatc gttctctaga agaactgcat
     6541 catatgcttg tatcgaaaga aatcactgta caagatttga caaaaacaac atttgaacga
     6601 atcaaagaaa cagagcctga ggtcgaggca ttcattacat tgaacgaaga aaaagcattg
     6661 gaacaggcaa aagctttaga tgaaaaaggg attgatgaaa gcaacgtcct tgctggtatt
     6721 ccaattggta tcaaggataa tatcgtaaca aaagatatct tgacaaccgc tgcatctaag
     6781 atcctttaca attttgaacc aatttatgat gcaacggtca tggataaaat ctatcaagca
     6841 gatatgatcc ctgttggtaa gctaaacatg gatgaatttg ctatgggtgg aagtacagaa
     6901 acttcttatt tcaagaaaac aaaaaatgct tgggacccaa caaaagtacc tggtggctct
     6961 tctggtggtt ctgcagcagc agttgcagct ggacaaattc cagtttcttt aggtagtgat
     7021 actgggggaa gtatccgcca accagcagct tttaacggca tcgtagggat gaaacctact
     7081 tatggccgtg tctcacgttt tggtttgatt gcctttgctt catctcttga ccaaatcgga
     7141 ccaatgaccc gtacggtgaa agataatgca ttagcattga cagctatcag cggttatgac
     7201 gaaaaagacg gtacttcttc aggcgtttca gttcctgatt ttgcagaggg actgacaggc
     7261 gacatcaaag gaatgaagat tgctttacct aaagaatact taggcgaagg tgtggcccct
     7321 ggcgtaaaag aagctgtatt gaaagcagca gaaacattcc gttcattagg tgctgttgtg
     7381 gaagaagtca gcttgcctca ttcaaaatac ggtgtggctg tttattatat cattgcctca
     7441 tcagaagcaa gttccaattt gcaacgattc gatggtatcc gctacggtta tcgctctgaa
     7501 aatgtgaaat cattagatga tgtatatgtc aactcacgtt cagaaggatt cggtgaagaa
     7561 gtaaaacgcc gtatcatgtt aggtacattc tccttaagcg cgggttatta tgacgcttac
     7621 ttcaaaaaag caggacaagt ccgtacattg atcaaacgag attttgaaaa agtctttgca
     7681 gattatgact tgatcatcgg gccatcttca ccaacagttg cattcggaat cggtgagaac
     7741 attaacgatc caatcacaat gtacatgagt gacatcttaa cgatccctgt caacttggct
     7801 ggacttccag gtatgtctgt accggcaggt ttatcagaag gattaccagt tggattgcag
     7861 atcatcggta attattttga tgaaaaaacg atgtatcaag cggcatatgc gtttgaacaa
     7921 gcaacagaat tccataaaca acaaccagct attttaggag ggaaagacga atgaattttg
     7981 aaacagtcat tggactagaa gtccacgtcg agttaaaaac caactcaaaa attttctctc
     8041 cagcgccagc tcattttggt gcagaaccaa acagcaatac aaacgtgatc gactggggtt
     8101 atccaggcgt gctgccagta atgaataaag ctgcgctgga attcgggatg aaagcagctt
     8161 tggcattaaa ctgtgaaatc tctaaagata ctcattttga ccggaaaaat tacttttacc
     8221 cagataatcc aaaagcatat caaatttccc aatttgatca accaatcggt catgacggat
     8281 ggatcgaaat cgaagtagaa ggtaaaaaga aaaaaatccg gatcgaacgt gtccatttag
     8341 aagaagatgc tggaaaaaac atgcatggta ttggcggtta ttcttacgtt gatttgaacc
     8401 gacaaggcac gcctttgatc gaaatcgttt ctgaagctga tatgcgttca ccagaagaag
     8461 catacgctta tctagaagca ttgcgatcaa ttatccaatt tacagaagtc agcgatgtga
     8521 aaatggaaga aggctctatg cgctgtgatg ccaatatttc tcttcgccct tatggacaag
     8581 aagagttcgg tacgaaagca gaattgaaaa acttgaactc tttatctttc gtgaaaaaag
     8641 gtttagcttt tgaagaaaaa cgtcaagcaa aagttcttct ttcaggagga gaaatccaac
     8701 aagaaacacg ccgttttgac gaaacaacga ataaaacagt tttgatgcgt gtcaaagaag
     8761 ggtctagtga ttatcgttac ttcccagagc ctgatatccc gaaattcgtg atcgatgacg
     8821 aatggattca aaaagtaaaa gctagtttgc ctgagatgcc agcttctcgt cgcgaacgct
     8881 atatccgtga gtttggactt ccagagtatg acgcaatggt attgacactt acaaaagaaa
     8941 tgtctgattt ttttgaagct gttttggaaa atggtgcaga tgccaagcaa gcatctaact
     9001 ggttgatggg tgaagtttct gcttacttga acagtgagaa actggaatta gctgagacaa
     9061 aattgacacc tgagaactta gcaggaatga ttcgtttgat cgccgatgga acgatcagct
     9121 caaaaatcgc caaaaaagtc ttccgtgaat tgatcacaaa tggcggagaa gcccaagaag
     9181 tagttgaaaa aaatggttgg gtgcaattat ctgatccgaa caaactactg ccaatcatta
     9241 atgaaatttt ggataacaac caacaatctg tcgatgattt caaaaatgga aaagatcgtg
     9301 cggttggctt cctagtcggt caaatcatga aagcaactaa aggacaagca aatcctggtg
     9361 tcgtgaataa gctacttaaa gatgaattag cgaaacgata gaggctgaaa atgaaaaaag
     9421 cacgcgtaat ttataacccc acctcgggaa aagaattatt gaaaaaaaat ttggcggata
     9481 tcctacaggc attagaagaa gcaggatttg aagccagcgc ctatgcaact acaccagaac
     9541 cagattcagc caagaatgaa gcatgtagag tcgcagaact aggttttgat ctagtagtgg
     9601 cagctggtgg cgatggcaca atcaacgaag tggttaatgg aatcgctccg cttgaacacc
     9661 gtcctaaaat ggccatcatc ccagctggta caacgaacga ctatgctcga gcattaaaaa
     9721 ttcctagaga taatattaaa gcagcagcag aagtgataaa aaaacaccaa accgtaaaaa
     9781 tggacatcgg cctttctgat aacagttact tcatcaatat cgctgcaggt ggctatttga
     9841 cagaattgac ttatgaagtt ccatcagaac taaagagtat ttttggttat cttgcttatc
     9901 ttgcgaaagg agcagagatg ctccctcgag taaagccaat caaaatgcgc ttgaagtatg
     9961 atgaagggga atatatcgga aatgcttcga tgtttttcct agggctgacg aactctgttg
    10021 gaggattcga acagatcgcg ccagatgcca aattggatga tgggaagttt tctttaatca
    10081 ttgtgaagac agcaaatatc tttgagatct tgcatattgc agcactcatg ctgaatggcg
    10141 gtcgacatgt cgatgatcca agaataatct ataccaaaac aagcagtttg ttcgttgaaa
    10201 cagaagatga tatcaatcga ccagtaatga tcaacttaga tggtgaatac ggcggggaag
    10261 caccgatgca tttccaaaat cttcatcaac acattgagtt ctttgccaat accgatcaga
    10321 ttcctgatga agcaatcaca ggtacagatg aagaagaatt agaggttgtc agcaaagaat
    10381 tcgtaaaaga agtcgaacgt ttaacggatg aagatatcga cggtgacgga aaaatagcag
    10441 acgaaacacc taatcaaaag aattaatcaa aagccggtgc tgaacgtagt aaggtcagtg
    10501 ccggttttag ttgtgtaatt agctgattta tagtagagtt cagtaggatt gaaaaatagg
    10561 cttaaaggag caaaatatga cagaacagca aatagtaaag aaaaacgaac gatatacagt
    10621 cgaaatcgtc gacttgagtt atgaaggaat gggggtagca aaaatcgatg gttatccatt
    10681 atttatcgaa aatgcactcc ctggagaaga agtagaaata ctagtagtaa aagcaggcaa
    10741 taagtttgga tatggaaaag tcgaaacatt tattacacaa tcacctgatc gtcagcctgt
    10801 agaaaacaat gcattacttc gcataggtat cgcaccttta gcccatttga agtatgaaca
    10861 acagcttgtc ttcaaacaaa aacaagtaga gcatgtaatg gcgaagattg ctaagatgcc
    10921 agaagttcct gtacttccaa cggtcggaat ggcagaacca ttcggctacc gtaataaagc
    10981 acagatccct gttcgccgta tcgatggaca gttagccacc ggtttttaca aaaagaatag
    11041 ccatgatcta gtccctatcg aagattttta tatccaagat cctgtcatcg atcaagcaat
    11101 caaagtagtc agagatatct tgcaacggtt ccaagtacga ggatataatg aagaaaaaaa
    11161 tgaaggacag cttcgtcaca tcattatccg tcgtggtcat tattcccatg aaatgatggt
    11221 cgttttagtt actcgtaaag aaaaattctt taaatccaag gaaattgctg aagctattca
    11281 tgaagaattg ccagaagtgg tttcggtcat ccaaaatatc aatgaagaaa aaacaaatgt
    11341 gattttagga gacaaagaaa aagttttata tggcagagac tatatcgaag atcagctatt
    11401 aggaaaaacc tatcgaatct cagcaaagtc attttatcaa gtaaatactc aacaagcaga
    11461 gtatctgtac caaacagcca tcgagtttgc cgagttagat aaaaaagaca ttgtgatcga
    11521 cgcttattca ggaataggta cgattggctt gtctttagca gacagagtgg ataaggtgta
    11581 tgggatggaa gttattccag aagcaattga agatgcacag ttcaatgctt taaataataa
    11641 aatcgaaaat gttcattatg aagtcggaaa agcagaaaaa gttatgaaaa aatggcaaga
    11701 aaaagggatc aaaccatctg tgatcgtagt tgaccctcca cgaaaaggat tagatcatca
    11761 ttttattgaa tcagcaatcg aaatgtcacc agaacgaatc gtctatatct catgtaatcc
    11821 ggcaactttt gcaagagatg ctaagttatt tgcagaagca ggttatgccg tgaagaaagt
    11881 tcagcctgta gatcttttcc cgcaaaccca tcatatcgaa ttagtcgctt cattcgttaa
    11941 aggagaataa aaatggacca aaaagaaaaa atgaaagaat tgatcaaaaa gaaacaaggt
    12001 ggaggccgat ccaaacaaat ggaaacacct aagaatgatc gtaaaaacat gagaaaaggc
    12061 cctaaaatct ttaataaata gacgtgctaa agaaagacta agaagtgtta acttttgtta
    12121 ttgtttctta gtctttttag ttagtctaca ttattttggc gatttgccaa taaggactgt
    12181 tgagttgtgc aaaatcaaat ctgggtcaga atgaacgtca ttctgtctca gatttttcgt
    12241 tatttcaatt tgattaactg attgatcaaa aagattttta ttttcatatt ctcaaatgat
    12301 tcaacactaa atatacatgt ttgacgcgga aattattatt cgattttggc ggcatcaggg
    12361 gggatgacga cgtagcagac ccattatttt actcaagaaa atgtccgtgc ctattattta
    12421 gaatacgata gtgaacgtgc tgggagtttt gttcttttag agaaagtctc tgaagataaa
    12481 ttagttgtct tagattttga ctgtaacgaa aaatggcgaa atggaagagc accagcaagt
    12541 gattgaccgg atctacgaag cgacacgata tctgcctttg gatcgtttgt gcttaagtcc
    12601 tcaatgtgga tttgcttcaa cagaagaaga aaatgttttg tttgaagaac aatggaaaaa
    12661 atttcttttg tcaaaagcat tgcagaagaa gtttggggaa aatcattaaa tcatctagga
    12721 gagcttgata ggaaataaca ctatggtata atcagaatgc ttaatactaa ctactgaggg
    12781 agccttatga aaaagaaaat tcttaaattt atcggatggt tcttcggaat cattcttggg
    12841 atcgcgtttg caatctttct tgccttccag ctttccccca gacctggagc attcttgatc
    12901 aaccatatgt tttcaaatac tgttcaaatc actgataaaa aaacgttcga tcaagcagaa
    12961 aaaaacgtgg cgaaaaaaac agatttgacg tatgagtcca agtataaaga gaatacttat
    13021 gacatctact atccaaaaaa cagcaagggg cctgttcctg ttctattttg ggtacatggt
    13081 ggaggattcg tcggcggaga taaatcgggt gtcaaagaat ttgcgacaaa attagtttca
    13141 gacgccaaca tcgcagtggt tgcgatgaat tatgaattag cgcctgattc agaataccct
    13201 aatcaagttc ttcaagtgaa tgaactgatc aaagatttat tagctgaaga aaaagacgac
    13261 aaattgctag acatggaaca attatttttt ggtggagata gtgcaggttc acaaatcgca
    13321 ttgcaatatg cagctataca aacgaatgat gcgtatgcta aacaaatgaa aatcaaacag
    13381 ctacttccaa aagatagtct gaaaggaacc atttcttatt gcggacctgt tgatttgaag
    13441 ctaacagcac agcagcattc ggatgatcga tttatgaagt ttttcgtcaa aaccgttgcg
    13501 tggtcgttga ttggaacaaa agattggaaa acgagtgagc aattgaaaga agctagtgtg
    13561 gctgaccatg tttctaagga atttccgcca acgtatataa ctgacggaaa tgcatactca
    13621 ttccaggagc aaggtcaagc attagaaaat cggttgacag aattaaaggt acccgtgcaa
    13681 tccttgtttt atacagatac aaaaaaagag attacccatg aataccaatt tgattacacg
    13741 ctaaaagaat cgcaaaattg ttaccagcag acattggatt ttgtgaataa gtataagtaa
    13801 gaggttgtga aaaagccgtt tcgttttgag tatgaagaaa cgaagcggct tttcacaacc
    13861 tctctttcaa tacctttttg ttcttgggat aaaactttcc ctaacaatca atcgtgcagg
    13921 ataagtcaaa atgattggtt cgtcccgtat accgtcaatt cgctccaatg ccattcgaac
    13981 agctgcttgc ccaattgatt tcgcattgat ttgaacactg gtcaagccag gggagaggtt
    14041 tgcgattgtc tcgatattat caaagcttgc tacaacaatc tcttctccaa tttggatatt
    14101 tttttgtttc aatgcccgat agacaccaat tgccattgga tcgctcccga caagaatggc
    14161 agtaggaaga ggggattcat tttctaatag attagtcgca agtacctcac cttgtttttc
    14221 tgtccagcca cctagatgat aagacatata tttttctaag tgatgatcga tcataaaatg
    14281 ctgataggca cgcatcctct tttcatttat agaaatctgc ttcccaccga attcatccat
    14341 atcgacttta tatcccccga tataggctat tcttttatga ccattttcta agaacaattc
    14401 cagtacggtt ttagtcatcc gttcaagatc tgcatacacc atatcgacct cttgctggat
    14461 gtcagggtta tccacgacga ctatttgcat gttttgtttt aacaaggcgg taatcgattc
    14521 tttcgtaaca gtcccaacaa cgatcaccgc acctaactga tctagatctt cccattgttt
    14581 aggattatca gatagattat agattcgatt catccctaaa tgcagacgtc gagcttcttc
    14641 ttccatccct actcgcaaat gtttgtaata gatatcctgt tcttcgctcg ctttgccgat
    14701 cgtagagatc acaccaatcg aaggcattct ctttttgata tatttacgag tactgatgtc
    14761 ataatccaac gcagcagctg cctctactat ccgttgccga gtagtctgag ccacatggat
    14821 cgtctgatcg ttattcaaca cacgggaaac tgtagcagga gagacatttg cctttcgtgc
    14881 gacatctcta attccaacca tttttacaca ctcctcaaaa aaataaaaaa tttttattga
    14941 caaaattgtt tagtaaacag tatacaggaa ttataaaaaa aagtaaacgg tttccgtttt
    15001 gttgggagtg aaatgatgga tattacgcat agaaaaacag ataaaaattt catcttattt
    15061 gatgaaaaga gccgagtctt ccatttacga aatacatttc tttcttatct gataaagata
    15121 gaagaaagca atgtattggc gcatatttat tttggcaaac cagtcaagca gtacaaagac
    15181 aataaaaatt atccccgaag agatcgtgga ttttcaggaa atgttccctt aaatcctgac
    15241 cggtctctgt cgaaagatac actaccgcaa gaatactctt ctcatggcag catggacttt
    15301 cgaacgcccg catcgatcat tcagcgcaag aatggatcag acttactgga tctgagatat
    15361 gacagccatt atattacaga tgggaagcca gagatcgaag gcttgcctca aacttatgtg
    15421 atggacaagt cagaggcaca aacgttggtg atttcactta aggatagaga aacagcgatt
    15481 tatttcgatt tgttctatac catatttact gatagagcag tgatcacacg atctgtaaag
    15541 ataagaaatg aaaccggaga aacaatcaaa ctagaaaaag cggctagttt ccagctagac
    15601 tttgcccata ctaggcgatt tgatgaagtc attgctttgc caggagcaca tgtcaatgaa
    15661 agacagattt cacggcaatc tgttttatct ggaacaaaag tatttgaaag ccgtcgagga
    15721 acgagcagtc accatatgaa taactttatt gctttagttc atcatcatac gacagaaaat
    15781 actggagaag cgatcggact tcaatttgtt tattccggca accattcttt cgaacttgaa
    15841 aaagatcaga tcaatcaatt gagagtcgtc ggtgggatca acagccatcg tttttcgtgg
    15901 gaattgaatg caggccagtc ttttcaaaca cctgaaatga tcctttctta ctcaagtcag
    15961 ggattgaaca aaatgagtca gatccaccat gagttgctac gtgaaagaat cgcaagaggt
    16021 cgtcatcaat ttgctgaacg gccgattctg gtcaataact gggaagccac ttactttgac
    16081 ttcaatagtg aaaaaatcaa ggcgatcatt gatgaagcaa aggagctagg catcgaaatg
    16141 ttcgtattag atgatggctg gtttggaaaa agagatgcgg acaattcttc attaggagac
    16201 tggttcgagt atgaaggcaa gttgacaaat ggattacgtg aaatagcaga ttatgctcat
    16261 agcaaaggat tgaaatttgg tttatggttt gaacctgaaa tgatctcggt tgattctgaa
    16321 ctgtatcgaa cacatcctga ttttcttatg caggagccag gtagaatgcc ttctgcttcc
    16381 agatcacaac atgtactgga ttttacaagg ttagatgttc ggcagacgat tgagaaacaa
    16441 atgagaaaga ttttagatac gattcctctt gactacatca aatgggatat gaaccgttcg
    16501 ctttcagatg tctattcgat tacccttgac ccacagagac aaggagaagt agcgcatcgt
    16561 tatatgctcg ggctttatga actacttgaa catttgacaa ctgattatcc ggaaatctta
    16621 tgggaaggct gttctggtgg aggtggacgg tttgatgccg ggtttattta ttatatgccg
    16681 cagtcatgga caagtgataa cacggatgca gtggagcgta tgaaaatcca atatggaaca
    16741 agccttgctt atccgatttc gtccatcaca gctcatgtat cagctgtacc aaatcatcag
    16801 accgacagat ctacttcgtt gaaaacaaga ggagaaacag ctatgagcgc tgtttttggt
    16861 tatgaactgg atttgacaaa gctgtctcct gaagaaaaaa agcaagtaaa agaccaaatt
    16921 atatcctatc aaacgattcg tccggtgatc cagtatggcc attattatcg tttggcgagt
    16981 ccttttgaag aaaatattgc cgcatggatg tttgtgtctc ctaaacaaga tgaagctatt
    17041 gtgtttttag ggaggatact agcttcagct caaccagcgt tccatgaagt atatctgatg
    17101 gggttagatg atgaggcact ttatcaggaa cagacctcga agcggatatt ttcgggggcc
    17161 gaattgatga cagttggact ttacttcccc gattttcaag gtgatttcca aacagaactg
    17221 cttcatttca aaaagttatg agagagaagg aaaaaagtat gaaagcaaat ataaaatggt
    17281 tagatgatcc acaagttttt agagtgaacc aatcaaatgc acatagcgat catgtttgtt
    17341 atgcaacaag ggaagaagta gaaaccacat cctcccttaa acagtcttta aatggatcat
    17401 ggcgtttttg ttattcgcca aatccttcgg tacgaccaaa aacattttat aaaaaagatt
    17461 ttgatgcctg ttcttttgat gaaatcactg ttcctagtca tattgaatta gcaaattatg
    17521 acaagatcca ttacatcaat acgatgtatc cgtgggaggg acatgtattt cgccgtcccc
    17581 ctttcacgac aagccctgag aaagaggcgg aagggatctt ttcagctgct aaagacaatc
    17641 cggttggttc gtatttgacg acttttcgat tagaagaagg tctaaaggga aaacaaatct
    17701 cgattcattt tgctggtgta gaacaagcaa tgtttatttg gttgaatggt cattttatcg
    17761 ggtatgccga agattcgttc actccgtctg aatttgattt aacaccttat ttggaagaag
    17821 aaaatattct agctgtggag gtttacaaaa gaagtacagc tgcattcttg gaagatcagg
    17881 actttttccg attttttggg atcttcagag atgtcttctt agttgctcga ggacaaaacc
    17941 atttagaaga tctttggatc aaacctcgct tagatgacaa atgtcaaaat ggcaaacttg
    18001 aagtagattt gtcttttgag caagtagcca aaaacagtca gctgaatgta gcgatactag
    18061 atcctgatgg gaaggaaatc tatcaacagc aatatccgtt aaaagagcaa atgacgattc
    18121 gcatcgatgg atttgaaaat gtggcactgt gggggcattt tcagcccaat ttatataagc
    18181 ttgaattgga gacgatagac gaagaaaaaa gagtaacgga gtatattgac tattccttcg
    18241 gttttcgtaa aatcgaaatc aaagataaaa tcatctactt caatggaaaa cggatgatta
    18301 tcaacggggt aaatcgacat gagtggcatc cgaaaaaagg acgcgccatc actatagaag
    18361 atatggaaaa agatttggct atcataaaaa atgcaaagat caacggcgta cggacatctc
    18421 attaccccaa ccagatcact tggtattatt tatgcgacca agcaggaatc catgtgatgg
    18481 cggaaacgaa cctcgaatcc catggctcat ggcaaaaaat gggcgcattt gagccttctt
    18541 acaacgtacc aggtgcagta cctcagtggc aggcggctgt tcttgatcga gcaaaaacga
    18601 atttcgaaac attaaaaaac catccaagta ttcttttttg gtctcttgga aatgaatctt
    18661 atgcggaaga aaacattcga caaatgaacg attacttcaa aaaagtagac cccgatcgac
    18721 ttgtccatta tgaagggaat tttccaaatc cagcattcga agattcgatt tcagatgtaa
    18781 aaagctgcat gtatgcgcca ccagaagaga tcattgacta ccttgaaaat aaagcggaaa
    18841 aaccgttcat cttatgtgaa tatatgcatg acatggggaa ttcgttaggt ggaatggatt
    18901 cttacatgac gttgcttgat cgttatgaaa tgtaccaagg aggctatata tgggatttta
    18961 aagatcaggc gatccaagtg actgatccaa tcacaggcag agaagtgatg cgctatggcg
    19021 gtgactttga cgataaacca agtgactatg aattctcagg gaatgggatc gtttttgcag
    19081 atggacaaga aaaacccgcc atgcaggagg taagatatta ttatgaaaaa tacagtaaat
    19141 aaaagtcata tggatacgga aaaagttgca atcgtcttcg gcgactgtac attaggtgtc
    19201 aaatcgggga atacgcatta tattttttct tatacaagag gcggactgga atcgctcaat
    19261 aaaaacggaa aagaatggct ataccgggaa acacttccag ttttttggcg agcaacgacc
    19321 gataatgaca gaggtagcgg atttggattt aaatccggta tgtggttagg agccggattg
    19381 tatccaaaag tcaaagaaat agaagtactg gtggacaata aaaagatcga tttgccgatt
    19441 gctccagtaa ataatcaata ttcaagtaat gaatacgctg aaacggttga aatcattttt
    19501 acactggaaa tcaacacagt accaaaaacc caagtgacaa tttcctacca aatcgctgga
    19561 gagggtaaaa tgcttgtcaa tatgcattat tatggacaaa aaggattacc ggaattaccc
    19621 gcattgggtt taaggttcat tatgccaacg aaagcaaaat tttttgagta tgaaggccta
    19681 tcaggggaaa catatccgga tcggatggct ggtggtgtcg aaggtattta taaagtggca
    19741 ggactccccg tttccccata ccttgttccc caagaatgcg gcatgcatat ggaaacgaaa
    19801 tgggtagaac taacaagaaa tcaaacgttg gataatacgg ataaagatca agaagatttt
    19861 tcgttgcgat ttgaacagca ggatacgcca tttgcttttt ccgctttacc attcaaagca
    19921 gaagaactgg aacaggcaac acatatagaa gaactaccgc cagaaagacg aacggttctt
    19981 acgatttttg caaaagtaag aggagtcggg ggtatcaata gctggggagc ggatgttgag
    20041 tcaccttacc gaatcagcag cgaagaggac catgaaatcg gctttattat tcagtagcaa
    20101 tctttagatt attatttgaa aaacggagtt tacaagacaa agctgcttta ttctatatgt
    20161 catgaacaag agagtaaaaa agttctcaga tacttttgtt catgactatt ttcttcaata
    20221 aaaccaattg ttatttttct gaaaaacaaa gttaactatc agaaaattaa aaaaagtttc
    20281 ataaaaatga ttgacagtcg atttttttat gtctatacta ggtacaattc ttacatcagt
    20341 ttggagcgat tttttatgaa atctagaaaa cttacaaaac tgaatacgta ttactttagc
    20401 tattttagat aggtttgtat tcatgacttc gtgggtacaa acaaaccagg tttgtatcta
    20461 tgatggctaa agatttttaa acacgactta cagccacgta gagaaattca tctatagtgg
    20521 ctattttata tttttgcagc gctttttaag gctcgttgaa gatgttcact gtagaggcaa
    20581 tttcctacag ggacatcttt ttttgttgat tatctgggtc ataagtagga aagaaggagc
    20641 gaaaatgaga acaacaggac acggtcattt agtaggaatt aatgtaaaac caccttgaaa
    20701 aagtagagaa aatcagccaa gatagataag taaacaaaag aaagcgggaa aaacaatgat
    20761 agtaattatg aaaaaagaag caacggaaga acaaataaaa aaagtcgtcg atcatatcga
    20821 aaaagaagga ttcaaagtct caatcgatca aggaacagaa cgagtagtga ttggactaaa
    20881 aggtgatacg cgaagtttac aagaaggagc atttacacgc tatgaaggag tagaaaatgc
    20941 tgtgcgtatt ttaaacacat acaaattgac ttctcgagaa ttccatcctg gcaatacagt
    21001 cgtggatgta gacggagtaa aaattggtga cggcagtttc gtgacgatgg ctggtccttg
    21061 ttctatcgaa ggattagacc aaatcagaga atgtgcgcga atggccaaag ccgggggcgc
    21121 aaaaattctg cgaggtggtg ccttcaaacc acgtacttct ccatatgctt tccaaggttt
    21181 agaagaagaa ggattgaaat acatccgtca agcggctgat gaatttggaa tgaaagtgat
    21241 tactgaagtg atggatgaag ggcatatcga tatggtggct gagtacagcg atatcctaca
    21301 gattggcgca cgtaatatgc aaaattttaa actgctatct gctgtcggta aaacaggtaa
    21361 acctgttgga ttgaaacgtg gaatttccgg tacgatcaat gaatggctta atgctgcaga
    21421 atacattgca gtcgaagaca aatctcctgt catttttatc gaacgtggga ttcggactta
    21481 cgaaacagcg acacgtaata cgtttgattt aagtgctgtc ccattgatga aaaaattaac
    21541 tcatttccct gtgattgttg atccaagtca tggtacagga atttgggaac ttgtaccacc
    21601 aatggctcgt gcgggtgtgg caagcggagc agatggcatg atcgtagaga tccatcctga
    21661 tcctgcaaat gcttggtcag acggtcccca atcgttgaat gaaaagactt actcacgcat
    21721 gatgaaagaa gttgcgatca tgaaagaagc aatggaaaag attcagacac tcgattaatt
    21781 ccagcttttt aaaagaaaga agtgaaacga atgcttactg tacatgttga taaccaaaaa
    21841 tacgatattc atattgaaaa aggcgcacta gaacaggctg gatcatggct tcaaacgatt
    21901 tggagacctc aaaaagtagc tgtgatcact gatacaaacg ttgctccact atatgcagaa
    21961 aaaatcatgt tccaattgag acaagctaat tttgaaccag tcctttcaat cgtaccggcg
    22021 ggtgagacca gtaaatcttt agaacaagca gctgagttat acgatttttt agcagataat
    22081 gattttaccc gcagcgatgg gatacttgca cttggtggag gaatggtggg tgatctagca
    22141 gcattcgtgg cctcgacgtt catgcgtggg atccatttcg ttcaaattcc gactacgcta
    22201 cttgcacaag tagattctag tattggcggg aagacagcag tcaatacaaa caaagcgaaa
    22261 aaccttgtcg gaacatttag ccagccagct ggtgtattga ttgatcctga ggttctaaaa
    22321 actttgccgg tacgtcgttt acgagaagga atcgctgaaa tcatcaaatc agcagctata
    22381 gcagatgcaa cgctttggaa acagctgaac gagctgaaag acgaaaaaga tcttctggaa
    22441 catgctgttc caatcattac tgctacgttg gaaatcaaac gaaaagtagt ggaagaagat
    22501 gtctttgacc aaggaaatcg acttatttta aattttggtc atacaattgg ccatgcaatt
    22561 gaaaatacag caggatatgg ggtagtcagt catggagaag gagtagctat tggaatggta
    22621 gccatcactt ctcatgcaga aaaaatcggc ttgtctccaa ctggatcgac agctgcttta
    22681 gaaaggatac ttcaaaaatt ccatctgccg attacgtacg atctaagtca tccagatgaa
    22741 ttattccacg cgataaccca tgataagaaa gcaagaggaa gccaattgaa tatcatcttg
    22801 ctagaaaaga ttgggcaagc aaaaatcgtt cctgttccca cagagacatt caaagattac
    22861 ttaggaggac tgtaagatgc gttatatgac tgcgggagaa tcccatggac cagaattaac
    22921 agccattatt gaaggactac cagcaggatt acctctgtca gaagaagaaa tcaatcacga
    22981 actcgctcgc agacaagctg gttacggcag aggaggaagg atgaagatcg aaaaagatcg
    23041 tgttcggatc acgtctggca tccgtcacgg caaaacaatt ggttcacctg tcactttagt
    23101 ggtagaaaat aaagattgga aaaattggac aactgtcatg tctgtagcag aagtgcctga
    23161 aaaagaccaa tcactgcggc gagttgcacg tccgcgtcca ggacacgcgg atttggtagg
    23221 cggtatgaaa taccatcatc gagatcttcg gaatgtgctt gaacggtcct ctgcaagaga
    23281 gacgaccatg cgtgtagcaa tcggcgcaat tgccaagaaa attctggaag aacttgacat
    23341 catcgtagca ggacacgtga tttgtcttgg tggaatccaa gcagaaatac ctggaaatat
    23401 ctcgattcaa gaaatcaaag aaatttctga agcgtcagaa gtacgagttg ttgatccagc
    23461 agttgagcaa agaataaaag atttgatcga tcagacaaag aaaaatggag atacgatcgg
    23521 aggaattgta gaagtgctcg ttggaggcgt tccagctgga ttaggaagct acgtacaatg
    23581 ggacacgaag ctagatgcca aaatcgcaca agctgtggtt agtatcaatg cctttaaagg
    23641 cgtagaattt ggggtcggat tcacttctgg ttcattacca ggctcagaag tgatggatga
    23701 gatcctatgg gaagaagaaa gaggctattt ccgacgtacc aataatctag gcggcttcga
    23761 aggcggaatg accaatggcg aaccacttgt cattcgcggg gtcatgaaac ccattcctac
    23821 attgtacaag ccacttcaaa gtgttgatat cgatacaaaa gaaccttaca aagcaagtgt
    23881 agaacgttcg gatagtacag cagttcctgc agctagcgtc gttgcggaaa atgttgtagc
    23941 cactgtggta gcgacagagc tattgtcaaa attcccaagt gattcgcttg ttgagttgaa
    24001 agctgcagtg aaggagtacc gtgactactt gaaacattac taaaaagggg gcaaataaga
    24061 tggcacaaca tgtttttatc gtggggcttg gactgattgg tgcttctatg gcactttgta
    24121 ttcgtcagac caatccagaa ctgatagtag ttggttggga tagtcagtca aaaacccgtg
    24181 agcaagcagt agaacagcag atcgttgatt acgttgcaag tgattttgaa acaggtgcag
    24241 aacaagcaga cgttatctta ttagccgttc ccgtccgtac aaccttagct tatcttgatg
    24301 tccttgaaag aatcaacttg tctgatcacg tcatcattac ggatgtcagc agtacgaagc
    24361 agcaagtcgt gacatccgct gagcagaaaa atcttcggtt tgtcggtgga catccaatgg
    24421 ctggttctca taaatcaggt atacaggcag cagatgctaa actatttgaa aatgcctatt
    24481 acatctttac acctttggaa aagacgaaaa aagatgttga aaaactgcaa gaactatttt
    24541 gcggaaccaa agcaaaatat gtaactctta cagcaagaga acatgaccgt ataacaggaa
    24601 tgctaagtca ttttccacat attctggcag ctggcttagt caatcaggca gaacaattca
    24661 atcaagagta tccgcgagcc aaacaactgg cagcaggagg ctttcgagat atcactagga
    24721 tcgcttcctc agatcctgtc atgtggacag atattttgtt gagcaataag cagatattat
    24781 tggaacgatt ggcagattgg caacaagaaa tgacacaaat agcagaatgg atcatgactg
    24841 agaaccaatc ggaaatcttt tcttttttca atcgggcaaa agaaagtcga gaccagttgc
    24901 ctgtccataa gcaaggcgcg attcctgcct tttttgattt atttatcgat gtgccagatg
    24961 aacctggcgt tattgcagaa gtaactggac tgatcggaaa agcgggagtt tctttgatta
    25021 atttgaagat ccaagagacc agggaagata ttttaggaat actgcaaatc agttttaaaa
    25081 accaacagga tttgttacaa gcaaaaagaa tcattatgtc taataccagt tatcattgct
    25141 ggatcaaata ggaggctgga taaatgcaat tattacaaca aatacatgga ttaagaggga
    25201 ctgttaggat accagcagat aaatcaattt cccatcgcag catcatgttt ggagcaattg
    25261 ctgaaggaac gacgactata caaaattttt tgcgcgcaga agattgtctg agtactttac
    25321 atgccttcca acaattaggt gtcgagatcg aagaagagga agaggtgatc aagattcatg
    25381 gtcgcggtag ccactcattt gtccaaccaa ccgaatcaat tgacatggga aactccggta
    25441 cgaccatacg tttattgatg ggtattttgg ctggacagcc ttttacgact actctgttcg
    25501 gggatgcttc gttgtctaaa cgtccaatgg ggcgagtgat ggaaccttta cgcgagatgg
    25561 gcgctgactt gcaaggagaa gaaaatgacc agtatctacc aatcactgtg acaggaaccc
    25621 gctctttatc acctatccga tacaatatgc ctgtagccag tgcgcaagtc aaatctgctt
    25681 tgctgtttgc ggcattacaa gcagaaggca catccgtaat cgttgaaaaa gaacgttccc
    25741 gtaaccatac ggaagaaatg attcgtcaat ttggcggaag gatcacagtg gaagataaaa
    25801 cgatcatagt gaccggaccg caaaaattaa ctggtcaaca gataactgtt ccaggtgata
    25861 tttcatcagc tgctttcttt ctagcagcag gacttcttgt tccggaaagc caactgttgt
    25921 taaaaaatgt cggggtcaat ccaacaagaa ctggtatctt agatgtgttg gaggagatgg
    25981 gcgcagcgat tacccagacg aatcacaatg aacagaacca atcggctgat ttaagtgtca
    26041 agacgagtca tttgaagaaa gcaacgattg gtggagaaat cattccgcgt ctgattgatg
    26101 aattgcccat tctagcttta gtggctactc aagcagaagg aatcacgatc atcaaagacg
    26161 cagaagaatt aaaagtaaaa gaaaccaaca gaattgatgc ggtggcagaa gaattacaga
    26221 aaatgggagc aaagataaaa gcgacagcag acggtctgat catccatggg ccgacacctc
    26281 tgcatggcgc aaaagtgtcc agcagaggcg atcatcgaat cggtatgatg ctgcaagtag
    26341 ctgctttgat agctgatgga ccttgcgaac tagaaggagc tgaagctgtt tcaatctctt
    26401 atccggcatt ttttgacgat ttagcagaac ttgtgtcagg aggagaagct catggctagc
    26461 attttgctga ttggtttcat gggcgctgga aaaacaacaa tcggtaaagg gttagctcaa
    26521 tgtttacaaa aagagtatgt agatttagat acaaagattg aggaacatat ccaattgtcg
    26581 attgccgaat attttcacta ttacggtgaa aaaagctttc gaaaagtgga gtcagatatt
    26641 ttaagaaagc tgtcaaatga agacaaaatt atcgcgactg gtggagggat tgtccaaagt
    26701 gcagaaaatc ggcgtttttt aaaaacacag ccgattgttc tttacttaga ggcagaagcg
    26761 gactgtctag ttgatcgtat ccaacaagat gaaacgtcta ttcgaccttt agctcttgga
    26821 aaaacaagag aagaaataaa agcattattg gcacaacgac tttcttggta tgaagaaagt
    26881 gcgacacatc gaatcaacac gaccaatcaa acaccagaaa agatcatcga taccattata
    26941 gaaaggatca aaagcatatg aacgtcagct atctagggcc agaaagttca ttcacttatc
    27001 aggcggcttg tcagctattt cctacagaac aattgactgc gtatgcttcc atccctgttt
    27061 gtttgcgcgc tttgtttcgc aaacaagtcg atctggctgt cgttcctgta gagaattcat
    27121 tggaaggagc tgttcatcac acaatcgatc ttctttccaa acatccagaa gtcgaagtta
    27181 aaagtgaaat cgttcttcct atcaagcagc agcttttagg caatccagca acaaagatta
    27241 caaaaatcct ttcacatcca caagcactag cacaatcaca acagttttta gaaacgcatt
    27301 atcctaatgt tcctttagta gcaacagaat caacaactgc tgcagcaatg tatgtagcag
    27361 aacatccgaa agaagacgca gcagcgattg cttcattaga aacagcacag catgttggat
    27421 tggagatctt agctgagaat atccaagata atgagttgaa ccagacacgt ttttggatcg
    27481 tcggtgaccg aaagatgacc agccagcagt cagctccagt aaaaatgagt gtcatcttga
    27541 cactacctgc taatcggcca gggatgcttc ataaaatgct ggcagctttt ggctggcggg
    27601 aaatcaactt aagtaagatt gaatcccgac cattgaaaac tagcttggga gaatattttt
    27661 tcgtgatcga tttgttgttg gaccgtccaa tgacattagt agagaatgcg ctccaagaaa
    27721 tcaagatgct cggaggagaa agtcaaatat tgggctgtta tccagtgctg acagtagaag
    27781 agacgcgtag atgaaagtca agaatactga ttgatttatt taacttaaaa taaaaaaatc
    27841 agcgcttggc tattctttat ttttgatatt ctgattgatt tttaggaaga agcggtagac
    27901 aaatcgttac agtcgttcct gtcccaatca ctgattggat cgttaagcca tatgcatcgc
    27961 catacattag gcgtatccgc tcatctacat ggctgatgcc gattcctgaa aaatgatcat
    28021 ttttttgttt ggtattttca gtatcgaagc catcgccgtt atcaatcact tcgaataaaa
    28081 gaagctgatt ttttgttttg ccgtacaccg tgatgaatcc ttgcgtttta ttgtgaaaag
    28141 catgaagata ggcgttttcg ataattggtt gaaggatcat attcggcata gataatgact
    28201 gcagttcatc aggaatcatt attttagccg agattctttc gccatatcgc atactcaaaa
    28261 gatcgatgta actttgtacg cttttgattt cttccttgac tggaatcact tcttcttttt
    28321 tcccaatcgt acttcttagt aaagtaatga aactgtcgat tccttgaact gcttcatctg
    28381 tttttttctg ccaaatcaaa aatttcaagc tagtcagcgt attataaata aaatggggct
    28441 gtatctgtgc ttgcatagtt tggatttcaa gcgaacgttt cttcttttcg tcttggatcg
    28501 tctgttcata atgatgctga gcattactta gcaattgatt gatcgtattc cctagcaatt
    28561 ggacttcatg gcttccttgt acaggaacag tagcttttaa gccatcttcc tctagccgtt
    28621 ttagcgaatc aatcaagcgg tagatcgatt ttgtgtgtcg atccaaatac ataaatacca
    28681 atgagccagc aaaagcaagt gtcacgatat tatacaaaat aatcggaaaa atcacgccca
    28741 tttggttaga taaagaatga agatcgactt gactgagtaa tgtacaatcc tggcggtaca
    28801 gaggaagacg ggtcaactgc tgtccatttg aaatcgttgt ttcacctact gttagttttt
    28861 tgaaaatcat tgatcggctc ccaattgtat tttcttcatt tgatgcgatg atcgtctgtt
    28921 cttcgttcat gaaggagatc ttcgatatct caggatttac tacagattga aaaagattcg
    28981 ctagttgctt ggatgagatc gataaaacag catatccgta aacgtgattc aatgtatttt
    29041 tcagtttttt gacaaagaag acgctgggtg ctttcttatc gcgaagagtc aatccgtggt
    29101 gtgcaaaaag catttgtgta tcagcagcac gttcattcac tctctgagca atcgcagatt
    29161 gaaaaaaaga atcggcagat acggcagtga gcatgtcgtt tccgacaaaa gttcttccat
    29221 tttctccaaa gatcaataca tcgtaatcga tcattggatg gatttttgaa agagagtcat
    29281 tcattttttg gattgttctt agttgctgaa gctgttccat atgttgggct ggatcattgg
    29341 aaaaatagct tttgacagct ggattagaat caaaagcggc taatgtattc tcgattgtat
    29401 cattcaaact ttcaaattca gattgaatct tttttagtac cacatcattc gattgattga
    29461 acagctggat ataggcatcc cttgatcgat gataaataaa caaagaaatg acgatggctt
    29521 gcagaaaaag accgatgaac aaaatgaaca gaagtttggc aaagagttca tgctctttga
    29581 ttttttttct cattttacaa aagttccttt acggtattga gaaggtgtca ttcctgtctc
    29641 ttctttaaaa actctggaga aataacccag ttcggtatag ccgactttct cacagatttc
    29701 cgtcaaagta agccgtgttt ccgtcaaaaa gagcttggct ttactgatac ggactttttt
    29761 tagatatttt gaaaagttga taccaaattt ttcagtaaag atggctgaga gatagctata
    29821 tgaaaaatga tatttttcgg ataaaagttg taaattgatt tctttctcat aattttcctg
    29881 gacgaactcg tagatcaaag tcaagatatc gaaattgtct ttttttgcat ttgcagaaaa
    29941 gtgggtgagt ttctcaagtg tttctttgac aagaatagaa aaatcttcga aatactcagc
    30001 atcggctaat aaagaaggaa gggcgatctt gagcaaagaa agttcttcat tcccaacatg
    30061 ctcgtcagcc aaagcgttga gtaatgcata gaaacgattg attgcctcat gtttcaaata
    30121 agctggatca ataggctgtt ccaatatcgt cgtgaagaaa ttctcaagtt ctttgacagc
    30181 taaagcgtag tctttatgaa cgatttttgt taaataacta cttgcttgct gctcgtggtt
    30241 ggttaataac ggaagaagtt catgctgata agcaaggttc tttcccttat gatagaaacg
    30301 ttgtcctttg gtgcaatttc tgatctcttg gaatatttca ttgatatctt ggattgcaaa
    30361 agaatcagaa acaacaaaaa aatcacctgg aaaaacttct tgacgaaact tttgaacaaa
    30421 cttcctaagt gtatctttgg aatcagtatt gattacatag ccggattctt ccgaaaaggt
    30481 agaaacagga taaatagtaa acgaatcgtc gctatccatc aatgaatggg tgatcgactg
    30541 tttttgttta gagccttgtt ttgaactgaa tggccgtttt tcggtatata acagaaaaac
    30601 aaggtgtgtt caaaaagaag agggtcaagc aatggacgag tttgtccagc aatctgctgt
    30661 gaaagattct cgcagatctg ctcataacga ttggtgattg ggcttttgtt gggctttttt
    30721 ttgatttttt gtgaaacggc agttaaggaa tttagaaaaa aatcaggact caatcgaggc
    30781 tttaaaagat aatcagcgac gccttcttta aaagaatctt taaccatatc aaattcatca
    30841 tagctagaga ggataaggat ctgtgtttca ggttgttgct gttttaatag ccgcgataat
    30901 tcgattccat ttaattcagg catcattaca tctgtgacca cgatatcgat tgcatgtacc
    30961 aaagaaaaat caagcgcttt cagcggactg gaaaaagtcc ctattaattg aaacccgtgc
    31021 tcagaccaat tgatcaactg tttcattcca tctaaaatcg tctgttcatc atcgataaga
    31081 attacgcgta ttggcatttt tttacccgcc ttaagaatag tcttgtaatt taaaaatact
    31141 tatttctagg agaaaatgca aggatatttt tattttaaag aaacctcacc atttcgatct
    31201 ttggtctaga tgctaagatt attccttttg tcatagttga tttgtatttt tttattattt
    31261 taggagaaat ttttgaagta tctgaataat aaagaaaccg ttttcaaatt ttggtatgat
    31321 gaatgtagca aacgtaaaca actaatggat ggaaaaggag cggataaaaa tgaacatcag
    31381 aaagttaatt gctggagtcg tttgtttagc ggcagcatca ggtttcttaa caggatgcgg
    31441 aagtgctagt agtacagcag acaaagaaga aaaagtcact gtctgggcgt gggatgagac
    31501 attcaatatc aaagcagtga atgaagcaaa aaaagtttat gagaacgaag aaacagagat
    31561 agaagttgtt acgatgtctc aggatgatat cgttcagaaa ctgaacaccg ctttagcgag
    31621 cgggaataca gaaggattgc ccaatatcgt cttgattgaa gattatagga tacaaggcta
    31681 tttgacttct tatccagatg cattcgctaa tttgtctgct attgtaaagg aagatcattt
    31741 tgcaccgtat aaatttgcgg taaacaaagt aggagataaa atatacggtg ttcctttcga
    31801 ttcgggcgta acagcaaact tctatcgtac ggatctgatg gcagaagcag gttatacaga
    31861 agaggatatg gagaatctga agtgggacga gtatattcaa gtagcgcgag atgtcaaaga
    31921 aaagacggga aagaaaatcg ctgaagtcaa tccttctgac cttggtcgag taaggatgat
    31981 tatgcaacag gcaggagaat ggtatacatc tgaagatggg gagacagtca cgatccaaga
    32041 caatgcatct ttaaagtatg gtcttgaatt gtttgccact cttttgaaag aagatctagt
    32101 cgaacaaacc tctgactgga atgctggagt aactgcgata caatcaggag cagtagcttc
    32161 tagcccaact ggcgcttggt attcttcaac gatccaagga gcagaaaacc aatctggaaa
    32221 atggaagatt gcaccaatac ctgcacttcc agaaaatctg caaaaagcgc aagcttctaa
    32281 ccttggcggc gctggctggt atgtgatcaa aggagtagct ggagaagatt cagcgaaaga
    32341 tttcctagaa gcaacgtttg cttcgaatga agaactgatg ggcactcttg cgaaagaaat
    32401 cggactagta tctacgatgc ttagcgcgaa tgaacaagaa gcttatcaag aagcttctga
    32461 attttacagt ggtcaaaaga tctttgacga tttttcaaca tggacatctg aaataccaga
    32521 agtgaattac ggacatgaga cctatgcaat tgaagcagtc gtagctgaag ctcttcatcg
    32581 aatcatcaat ggagaagaga cagacaaggt actagcagat acacaaaaac aagtagaagc
    32641 acaattggct aattgatcgg tggtaaaaaa gtgaatttct tcgctttttc atcagacaaa
    32701 cgaaaagaca aaaaagatca cgtctgcctt acagatattg tctttcttgt cttttcatgt
    32761 agataatcaa tgatagaatt ggagggaaaa gaatgaagca gaaagattta ttcaaaaacg
    32821 ggcatgcttt tttaatcttg ccagtcattt tgatatcaat catggttttt attccgatgg
    32881 ttttggcttt gatcacgtct tttcaaagtg gtccccctgt caatatgaca tttaacgggt
    32941 taggtaatta tcagcggatg ttgacagatg caacttttaa aaaagctttt tttaatacat
    33001 tcctgtattt acttgtacaa gtaccgatca tgttgtttct agcacttttt atttccaacc
    33061 ttttaaatga taaaaagttg aaatataaag ggttgtttcg aaccgctatc tttcttccat
    33121 gtatcacttc gatggtcagt tattctctga tcatgaaaag tctattttct caatctggcc
    33181 ttgtaaacaa cttgttgttg ggcattggta tgattgacac accgattggc tggttgactc
    33241 atccaatttg ggcgagagtt ctgatcattc tttcgatcac ttggcgttgg actggataca
    33301 atatgatttt tttcctttca ggcatgcaaa atattgattc ggagatttat gaagcagcat
    33361 ctattgacgg tgcttctaaa tggcagcagt ttatcaaagt gacgatccca aatttaaaac
    33421 caatcatttt gtttacttcc attacttcga cgatcgggac attgcagctt tttgatgaag
    33481 tacaaaatat tactggcggg ggaccagcaa atgcaacgac caccctatct caatacatct
    33541 ataatttgag ttataaattc acgcctaatt ttggatatgc ggcagcagtt tcctttgtta
    33601 ttgtttcctt tattgtgata ttgtcgataa tccaaacgaa agtaggagga gaaagtcgat
    33661 gaaaaatatg gtagaaaaag aaacaatcga acgccccaac gtcaaaaaac aagcattaga
    33721 acttcgttcg acaattgggt cagtattaaa gtatacagca ttaagcatcc tatcatttat
    33781 ttccattttc ccttttgtct ggatggtttt gggaatgacg aatacacccg tggatatcac
    33841 atcgggaaaa ataaagattg gcagtaactt gatcatcaat tttcaaaatt tgttttccaa
    33901 tgagctgaat tttactcgtt ccctttggaa ttcagcgatg atcgctttgg tgaccactgt
    33961 gtttgcattg ctgatttcgt caatggcagg atatggattt gagatttatc aaagtaaacg
    34021 aaaagaccta attttcaata ttctactgct atcgatgatg gttccttttg cggcattgat
    34081 gatcccttta tatcgaatgt tttcacaatt gaacggaacg gttttgggaa tcaacagttt
    34141 cttggttgtc atcctgccat ctgttagcac tgcttttttg atcttcttct ttcgacaaaa
    34201 tgttaaatct tttccaaaag atctagtaga agccgctcga ttagacggtc taaaagaact
    34261 aagtattttt tttagagtgt atatgccgac ggctaaaaat acctatgccg cggctgcaat
    34321 catcacattc atgagtagct ggaacaatta tttatggccc ttagtagctt tgcaatctcc
    34381 tgataaacgg acggttccgc ttgttttatc tgctatgggt gcttcctata caccagacta
    34441 tggaatgatc atggctggat tagtcatcgc tactttacca actgccatcg tattttttgt
    34501 tttgcaaaag caattcgttc aaggaatgat cggctctgta aaataaaaaa acacaataag
    34561 cgcgttcctt caaagtcatt tttccaagta aaaagatact tttgggacgt gtgttatttt
    34621 atgttgagaa aaattaaatg ataacgtttt aagtttgtgc tcgaaaactc tttcatttta
    34681 aggtaaactt aaagagtaaa ggaggaggat acatgctaaa aaattttaaa gaagtgattg
    34741 ctgctatatt gccgatgact gtcctgattg tcattttaac agaattaggc agaagtcgcc
    34801 catcctctat aggtgggtga tgaatgccgt tcggtatgag ggttaccgtt ggtaactcga
    34861 agaccgacaa ctaaggcgta ttgccttttt attttgttcc atgttacaat cagtctatac
    34921 aagaaatgag ttgatataac atggaattag atacaaataa tcattcagta tttcttcttt
    34981 attaccacct tgttttagta acgaaatatc gtagacaagt gattgatgat gaaatatctg
    35041 attatgccaa aactactttt gaaagaattt cagaatcgta tcacattact ttagtggagt
    35101 ggaatcatga taaagaccac gttcatatta tgttcaaagc tcaacctaaa acagaattga
    35161 caaaattcat caatgcctat aaaagtgcca gctcaagatt gataaaacgt gactttccaa
    35221 gagtcaaaca atttctttgg aaagaaatgt tctggtctaa aagtttttgt cttctgacaa
    35281 ctggtggcgc accaattgat gtgattaaga agtatatcca aaatcaagga aacaatcata
    35341 aatagaaagt aggtgaactt tatggaacaa ctaaaagcat acaaattcag aatctatcca
    35401 acagaggaac aagaaatctt ctttgccaaa tcttttggtt gtgtccgtaa ggtctacaat
    35461 ctaatgcttg atgaccgaaa aaaggcgtat gaagaagtta aaaatgattc ttctaaaaaa
    35521 atgacttttc cgacaccagc taaatacaag aaagaatttc cgtttttgaa agaaattgat
    35581 agccttgctt tagccaatgc ccaacttaat ttagataaag catacaagaa tttttttcgg
    35641 gataaatccg ttggatttcc acgtttcaaa agtaagaaaa atcctgttca gagttataca
    35701 acgaataatc aaaatggtac agttgctttg attgatagta aattcatcaa agttcctaaa
    35761 ttgaagtcat tagtaagaat taagcttcat agacaaccaa aagggattat caaatccgct
    35821 acaatatcac gtcattctag cggtaaatac tatatttctt tgttgtgtaa agaagaaatt
    35881 atcgaattac ctaaaactaa ttccacaatt ggtattgact taggtattat ggactttgcg
    35941 attctttctg atggacaaaa aattgataat aataaattca cgtccaaaat ggaaaagaaa
    36001 ctaaagcgtg aacaacgcaa attgtctagg cgtgctttat tggctaaaaa taaaggtacc
    36061 aatctatttg aagctaaaaa ttatcaaaaa caaaaacgca aagtagctag attgcatgaa
    36121 aaagtaatga atcaacgtac tgattttctg aataagttga gtacagaaat gatcaaaaat
    36181 cacgatatta tctgtattga agacttaaac acaaaaggta tgttgcgaaa tcataaatta
    36241 gctaaaagta tttctgatgt ttcttggtca aagtttgtga ctaaattaca atacaaggct
    36301 gactggtatg gacgaaaaat catcaaagta gataaatggt ttccgtctag tcagatttgt
    36361 tcagagtgcg gacataaaga tggcaagaaa tctcttgaaa ttcgagaatg gacttgtcct
    36421 gtttgtcata ctcatcatga tcgtgatatc aatgctagta tcaatattct gaccgaaggt
    36481 ctaagactac attctatggg attggcttag atccaatcat caagaaccgt aggaactacg
    36541 gggatagctt ggtaaataag agacaccgct gttagtaaag aaatacgcta tcaagtatgc
    36601 tctattccca agaagctccc acttcaagcg ttaagaaccg tcaggtttag ctaagtggtg
    36661 agtagttcac cgtttgtttt tgcaccgtta gaaggagaag acctcgcttc atttctggtt
    36721 ggtgcggcaa tcatgatgat cggaatgacg ctctttttgt ttggagcaga ttattctatg
    36781 atggaagtcg gagatctagt agggaaatat atgatcaaaa agaaaagttt gaccgttttg
    36841 gtcagtttgg gatttgctat cgggatcgtg atcacgattg ctgaaccttc cgtgcaagtt
    36901 ttgggacaac aagtatatga tatatctgac ggagagattg gcagagtgtt gctgattggg
    36961 atcgtcagcg tcggcactgg agtatttcta gcctttgcgc tgcttcgggt cgtattcaag
    37021 ctatcctatt atcaattgat ggctataggc tacataggcg ttttgatcgc ctcatttttc
    37081 accagttccg aatttatgcc gattgctttt gattcggggg gagtgactac tggtccagta
    37141 actgttccat ttatcttggc ccttgccgga gggatgacta gtatgatcaa acaaaagaaa
    37201 aatgaaaatg acagttttgg aatggtcggt attgcttcgt tagggccgat tcttgccgtg
    37261 atgattttgg gggtgatctt ccaatgagtt tctggcagca agtgatagat ggaatcgaaa
    37321 tcgttaccat ggaagtcatt ttggcagtgg cacctatcgt aatcctattt gttttaatga
    37381 atatcattag ttttcgtatg cgcaaaaagc gtttctttga cattatgaaa ggtttcttga
    37441 taacgactat tgggttgatc cttttcctgc acggagtcaa tatggcttat gtaccggtag
    37501 gacagcattt aggcagtgct attgcgggat tgccttacaa ttatatactg gttccccttg
    37561 gctttttaat ggggttctta gtagggtttg ctgagccggc gattcatgta atgatcaaac
    37621 aggttgaaga attgagcgag gggagaatcc gatcgaaaat catgctgatg gtaatttcaa
    37681 ttgggatcgg cgcagcagta ggattgtcta tgtggcgttt gctcgtcgga ttctctcttt
    37741 attatttctt gatcccagga tacgggctag catttatttt aggacgaaaa gtggacaaga
    37801 tctttttagc aatggctttt gataacggtg gagtagcaac gggcccgatg tgttcgacat
    37861 ttattttatc aatgagcgtg gcaatcgctg cacaaattga aggaagagat ccgatcattg
    37921 acgggtttgg tgtggttgca atgattgcat tgacaccaat attatccact ttagtactag
    37981 gatatattta taaacgaaaa gatgaaaaag acagaatcga aaaagaaaga ttgcaaaaat
    38041 tgggggaaag caaatgaaag atcttgtagc attgaatctt cacatgattg tgacgatcgt
    38101 tgacggagga atgggcgaag atgttatcga cttttcaaaa gaagcaggtg cagcaggcgg
    38161 aacgatctta catggaagag gttctggtgt acatgattca gggaaatttt taggtttgga
    38221 catttcgcca gaaaaagata ttgtgttgac attagtaccc gatagtttga cgaataaagt
    38281 aatggatgcg attggtcaag gcatcaacat cgatattcca ggtaatggta tttgttttag
    38341 tattgatgta gacaaagtca ttggtatcac taagattcat caatatcgag aaattatcgg
    38401 aatgcaagaa cttttagatg aaaataacga tagagaaaag tctgaatcat agacaagcgt
    38461 ttgaagagga aataaacaca aacaagaagg cggatgagcg ttatttccgc ttttttgttt
    38521 gtatttttct cgtgaatact tatgaaatag taaattttta tgaaattcta ctctaagcat
    38581 cagactttct tttcaaaata tgctatttta aaaatgatga aaaaaggggg agaaaaatga
    38641 attttttagt tgttttaaaa gatactctga ttgagcgatt tacatttcta tatgttcaat
    38701 tgcaggaaaa taaaacattg ctgcattttt caccagagtt ccatctagag gggctaacat
    38761 taggagaaat ctatcaagca gaagggctgt cggctgtttt gcactttttt aaggtagaat
    38821 tggaattacc agtaaaagat tatatcgagc tttctcttga atcttgggat cgagcagtgg
    38881 ttgaactgtt tccagaaggt ttaatgatcg atacaaacga tgggaagatc catctgactg
    38941 cagcagaact gcaaaaacaa atgcgttatc aaatagatga cgaaaattct ttttctattt
    39001 ttagacgtca gcaaaagatt ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc
    39061 tgatcaaaac gacacagctg ttgaaaaaat atccaggtct gcttcacacg tctattcaat
    39121 ttcctcaatt gattacgatg atccatcgtt ttatacgtat gcaacagcta ccatctaaga
    39181 agctgtttct tccattgacg gacacatttc gagtagtcaa cagagaagat aaaaagaaag
    39241 tgattgtgat tgactttacc agaaatcgga atgtccttca ccaagttgca atggaaaagg
    39301 ccaattaagg ctttttcttt ttggattttt ttgtcataat aaatattaga tgatggagga
    39361 gataatatga tgcaaacatg gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa
    39421 cttatgcagg gaagatatgc gaaatttgat cagttgaatc gcggtctatt gatcgctgcg
    39481 cttgtcttta gtttgctaag tcgttggttg ccctatagaa tcggacaatt gttgttcatc
    39541 gtctttttcg gatggatgat atttcgtttc ctatcaaaaa aaatctaccc cagactaaat
    39601 gagaatcaac gttatttgaa atatcttgat agagtcaaag aaaaatatca tcagataaaa
    39661 aacaaaaagc aagtcaaaat ggatcgaaaa agttatactt tttttgagtg cccgaattgt
    39721 catcaaaagc agcgtgcacc taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt
    39781 ggtataaaat tcgaaacgaa tgtttaaggg agaaaagaat gaaattagta ttattacgtc
    39841 atggcgaaag cgaagcaaat tttgaaaatt actggacagg atggctagat gtcgccttaa
    39901 cagaaaaagg aaaagaacaa gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc
    39961 aattcgatgc cgtttatacc tctgttttga aacgtgcgat tcttaccgga caagtcgcac
    40021 tagaagaaat gggtcagtta tggcttccta taataaaaac atggcgtcta aatgaacggc
    40081 attatgggga tcttgtgggt aaaaacaaag ataagatgaa agaaatatac ggaaaagatc
    40141 aagtaaaaac atggcgcaga ggatatacgg aagttccgcc gttaaccgca gaaaatagat
    40201 ttgaccgcag atatgatcac ttggatccgc atttgatccc atacggagaa agtctggaaa
    40261 tgacagtgaa tcggatcatt cccttatggc aagatcatct agcaccaaaa ctaaaagatc
    40321 ggcaagattt actagttgtg ggtcatggta atagcatccg agcactgaca aaatatttag
    40381 aagatgttcc tgaagatcaa atggatacga tcgatatccc aaatgcacaa ccaatccagt
    40441 atacattagc agatgatttg acaattctgg acaaaaaaat actttaaaga acacctttat
    40501 ctaaaagtga gagatagaaa aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt
    40561 atatatggga ctttgatggt acattgtttg atacgtatcc cgcaatggta gatggtgcat
    40621 ggcaagcact aaaagatttt ggtatcagta tggacaaaaa agaaatctac ttcaaaatga
    40681 aaaagtattc tactagttat ttgatcaatg aatcaaactt gaatgccaga gagtttaatg
    40741 aattatttca ccgatatgaa aaagaaagta cggaagtttc ccgtccattt ccagaaacga
    40801 aacaagtact ggaaatgcta aaagacaacg gcggtcgtca tttcatcctg acccaccgtt
    40861 tgacagaatc aacatggggc ctgttaaaag aacatcgatt ggcacatctg attgaggaag
    40921 tagtagggat cgatcaggac tttcctcgta aacctgatcc agcatcttta aattatttga
    40981 tcgatacttt tcatttagaa agaacagaca cgatgatgat cggcgatcgt cgtttagata
    41041 tcgaagcagg gaaaaatgca ggagtcgcta cttgtcttta cgacatcgat cattttctgg
    41101 gtgagatccc ggcagattat gtggtaggaa atctgaatga aatccttacc ttgcagagca
    41161 tcattaaaaa gtagtatccc ctttcaaagg agatatgtta taattttcat aaaggagtga
    41221 taaaattata tggaaatcag aagacaaaaa gaagttgtag aagcatttca ttatgatatg
    41281 agattgccga accaagaagt agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa
    41341 acgatcgaaa actatccgaa agaacatagc atcgtaggag cacgcttaga gttccgttta
    41401 gtgtttgata catatgtttt atctggaagt gtgagtcaga tcaatcatat cctcaatcgg
    41461 aaaattgaag gtcaaaatga tgtcacacaa gaagaagtgg acgagctagt agcaccgtta
    41521 ttcgatatcg tacgccgaat gtcttatgag gtgacagaga tcgccttaga tcagcctggg
    41581 gtaaaattga attttcaatc agaaaaacaa gaatagacag taaaaacata cctgcgatag
    41641 attaaagcta tcacaggtat gtttttttat tactaattgt aagcaaaaag aatgtcattt
    41701 cgataaaaat cgtctaaaat ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg
    41761 aagaatcatt ctcgggaacc tttacttcct tcgatggact tgtcattaat aatatgagga
    41821 ggagaaaaat gacaacactc atcacagttt tagtggttct agtttttgcg gcattattat
    41881 atgtgttcta tcgtatgcaa aagcgccata caaaattttc aacacgcgtc tttacagcat
    41941 taggagttgg tatcgtcttt ggcggtctgc ttcaattgat ctttggtgta gacgacaaag
    42001 tcgttactca atcaatggaa tggatcggga ttgtaggtca aggatatgta tcattcttgc
    42061 aaatgctagt tatgccttta gtgtttattt cgatcattgg tgcattcaca aaaatgaaag
    42121 tttctgaaaa gctagggaaa attagtgcga ctgtattgac ttctttactt ggaacaacag
    42181 ctattgcagc attgatcgga attggttcaa caatgctgtt cggactccaa ggagctaaat
    42241 tcacacaagg agctgcagaa acagcacgaa tctctgaact ggcaacacgt caggaaacag
    42301 ttgaaaactt atcgattcca cagcagattt tatcctttat ccctaaaaac gtttttgctg
    42361 atttagcagg tagtcgtgca acaagtacga ttggagtcgt tattttcgcg gcatttgtcg
    42421 gaatcgctta tctaggcgtg aaaagaaaag cgccaaaaga agcagcattc ttttcagatt
    42481 tagtcgacag tctgtataaa atcattatgc ggatcgtgac gcttgttttg cgtttgacac
    42541 cttatggagt atttgctttg atgacaaacg ttttggcaac aagtgatttt gcagctatcg
    42601 tcaatttagg taaatttgtc gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc
    42661 actcactgat tttgattggt gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc
    42721 cggtattgag ctttgcgttt acttcacgtt caagtgctgg agctttgcca ttgaatatcg
    42781 agactcagac aaaagcttta ggagtagatg atgcaacagc taatttctca gcaagttttg
    42841 gtttgtctat tggacaaaac ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg
    42901 tcgcaccagc agctggagtg aatgtcttta gtttggaatt tattttgatg cttgtggcaa
    42961 ttgtaacaat cagttctttt ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac
    43021 tgatcgtttt aggggcaatg aacttgccag tagcaatcgt tgggttagtt atctctgttg
    43081 aaccattaat cgatatggca cgtacagcag taaacgtcag cggtagcatg gtagctggta
    43141 tcgtgacaag tgcacgcatc aaagatttag atcgcgatgt catcaatgat gattctaaag
    43201 tgatcgatgt gaatgcatag tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa
    43261 taagaagaag tttcctaaaa atagtttctc aaatttttgt gaaatttcgg cttatttccg
    43321 agaagctact tctgaaacac cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt
    43381 aataagaaca gccaaacaaa aatagttcct catattttac gtttggttga gcttattacc
    43441 gcagatgcaa gcttttgaac accgtttaag aaagaggtcg tgagcctgcc gtttcactct
    43501 gagtcacaaa gagcaatttc aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt
    43561 gaccgaggag ttgtcaggag aacaccgttt accagaggtt gtgacttgtt tttagtcacg
    43621 acctcttttt ttatttattc catataaata cacattattt attccgaaaa gtgaaagcgc
    43681 aaataacaaa aacaaccaac ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat
    43741 tctgttgaga gctttcgtag attttcccat tttcctatgg taaactgaca aagttgagaa
    43801 tgttagaaca gatcgtttag gaggaaacag atgtttacga atgaaataaa aatcatgctt
    43861 tatgtggacg atgtagaaaa aaatgcaacg ttttggagag cagttggctt tgcggaaaaa
    43921 gatcgtcaag aaatggatgg gacgcttata attgaagtag gagttgagga cagtcaaacc
    43981 tcatttctgt tatatgatcg agcatttgtt gagtcccatt cacctggcac agctactagt
    44041 gccccatctt tgatgtttta cagtaaagat atctttggac tatataaaaa aatgcagacg
    44101 caatcagtca cattaggtga tatggtacag cttggagaag aatatgtctt taactttgct
    44161 gatcccgatg gcaactactt tgcggttaca ggtaaataat agaaataaag actttgtttt
    44221 tctgtgcgaa acaggaaagc gaagtctttt taattcaaat aataatatta tgtaaacaaa
    44281 aaactttcca tgcgtaatta tacacaagca aataaatcgt caaatgcgaa cgccttcttt
    44341 atactgttta ggaacgaaaa gaaggaagga agaaatacat ggattatcaa gtactgcttt
    44401 attacaatta tacaaaaatc gaagaaccag aggaatttgc caaaaatcat ttagcctttt
    44461 gcaaatcttt ggatcttaaa ggtcgtattc tagtagctga agaaggaata aatggtactg
    44521 tgtctggaac cgttgaaaac acgactgcct atatgaaagc aatgaaagct gatcctcgat
    44581 ttgctgatac gatgttcaag atcgatccaa gcgaaggaca tgcctttaag aagatgtttg
    44641 taagataccg tccagagctt gtaagtttaa agctagaaga ggacattgat ccaaaccaag
    44701 tgacaggtgc ctatctttca ccgcaagaat ttaaagaagc tattttagat gaacagacag
    44761 tggtgattga tgcacggaat gattatgaat atgacttggg acattttcga ggggctgtac
    44821 gcccagatat acgcagtttt cgagagcttc cagactggat ccgcgagcac aaagaagaat
    44881 ttatggaaaa acgggtagtg acctactgta ctggtggcat tcgttgcgag aagttttctg
    44941 gttggcttgt ccgtgaagga ttcaaagatg tcggacagct gcatggcggt attgctactt
    45001 atggtaagga tcctgaagtc caaggagaat tatgggacgg caagatgtat gtgtttgatg
    45061 aacggatcgc tgtcgatatc aaccaagtag ataaacaaat cgtcggtcgc gactggtttg
    45121 acggaacacc atgtgaacga tatatcaatt gtagcaatcc tgagtgcaat cgtcagattc
    45181 ttgcttccat tgaaaatgaa gaaaaacatt taggagcatg tagcatagag tgtgcgaaac
    45241 atccgcgcaa tcgctatgtt caagaccatg gaatctctcc agaagaagca gaactgcggt
    45301 ttacagcttt aattaaatag aaaacaatag ttttgattgt cgaccatttc caatatgcgc
    45361 tatactagcg ttgaggatgg tgatccacat gaaatcattg atttatgcta ttggggatgt
    45421 ccatggcaaa catgagcttt ttgaaaaact tattcactat tatgatccaa agatccatca
    45481 gcttgtttta ttaggggatc tcaatgatcg aggtcctcgt acaaaagaat gttttttatt
    45541 aggaaagcaa ttagtcgaag aaacaggagc catttacctc cggggaaacc atgaagaata
    45601 ttttttgcaa tttctgcaat caccggagga ctggtatgcg ccttatatga gaaatggtgg
    45661 aaaagaaacg tttgaaagcc tgattcaccc aggggcatcg gaggaatact ccccaactga
    45721 aatctcgatg atgatccaat cgagatattc agaactgatt aaatttttag tccagcgccc
    45781 cttgtattat gaatgggaaa attatctttt tgtccatgcc ggtgtggatt tgacaaagaa
    45841 agactggcgt aagacaagtc taagagactt tatttggata agggaaccat ttcatgaagg
    45901 gaaaaacaat acaggtaaga caattgtatt tggacatacg attacaccaa tgctccatgg
    45961 agatatgcag acaacagacc tctggattca ggatcataaa atcggaatgg acggtggggc
    46021 tgtattcgga gggtcattgc atggggttgt ttttgacaag gaaggtattc ttcaagatat
    46081 tgaatatcag aacaacgaag gaccttggca tggaatctga ctaatggaaa gaatgaagga
    46141 gctgtgactt tgttgcggct ctttttattt ttatagagaa tattgtttct ttcagtagtt
    46201 taacccagga taaaaagtat gatagaataa agaagcaatt tttagcagaa aagaggcatt
    46261 ttaatgacgg aaaatttgaa ccagacgatc attcagcttg tacaaaaaga actttcagac
    46321 tatcgtccta agcagttgac tactgtactt aatttgttaa atgaaggaaa cactgttcct
    46381 tttatcgcgc gttaccgaaa agaaatgact ggaagtcttg atgaagtcca gattcgtgaa
    46441 atcgaagagc ggtatgccta tcttgaaaac ttagaaaaac gtaaaactga agtgattcgg
    46501 ctaatcgatg aacaagggaa actgactccc gagttagaag cagaaatcac ccaagccgtg
    46561 aaaatgcaac aagtggaaga tttgtatcgt ccatacaaac aaaaacgacg gacaaaagca
    46621 acgattgcta aagaaaaagg acttgaacca ctcgcgaagt ggctgatgca gcttacagat
    46681 ggagaggtgc aatctgaagc agaaaaatat attgacaaag aaaaagaagt ctcttctgcc
    46741 gaagaagcgt tgcacggtgc tcatgagatt attgctgaac aagtcagcga taatgcaaaa
    46801 tttcggactt ggattcggtc ttatacgtat aacaaaggta tgtatgtcag caatgtcaag
    46861 gatgagcaag cagatgaaaa aggcgtatat gaaatgtatt atgactttgc agaacccgtc
    46921 cataaaatgg tttctcatcg aatattagct actaatcgtg gagaaaaaga agatattctg
    46981 aaagtattct ttcaagtaga tgaagcagca attttagctt acttggatcg ccaaatagtc
    47041 aaaaatcctg cctctccttc ttcttcattc gtcagagaag cctatcagga tagttataaa
    47101 cgctttattc agccagcaat cgagcgagaa ctgcgcaatg aattgactga aaaggcagat
    47161 gagcaagcaa tcgctatttt cggcgaaaac ctccgtaatc ttttgttgca accgccacta
    47221 aaaggtaaag ttgtcctggg atttgatccg gcttatcgta ctggatgtaa actagcagtc
    47281 gtagatgcga cagggaaagt tttagcaatc gaagttattt atcctcataa acctgcagcg
    47341 caagcaaaaa gagaagctgc tggaccagct tttatccaat taattaataa atatcaagta
    47401 gatatggtag ctatcggaaa tggtacagcc agtcgggaat ctgaactttt tgtggctgag
    47461 cagttgaaat ccgccgacca taaaacctat tacgctatcg tcaacgaagc aggcgcttct
    47521 gtttactccg ctagcgaaat tgcacgaaaa gaatttccgc atctacaagt tgaagaaaga
    47581 agtgctgttt ctattgcccg tcgcctgcaa gatcctttag ctgaattagt caaaatcgat
    47641 cctaaagctg tcggagtagg ccagtaccaa catgacgttt ctcaaaaacg tttggctgaa
    47701 caattggatt ttgttgtgga aacagcggtt aaccaagtag gtgtggatgt gaatacagca
    47761 agcccgcagc tcttgcagca tatatcagga ttgaataaaa caactgccca aaacattgtc
    47821 atttatcgtg aagaaaacgg agaattcacc gcacgaactc aattgaaaaa agtgccacgc
    47881 ttaggaccaa aagcatatga acaagcaatc ggattcttgc gagtccctgg tgggaaaaac
    47941 attttggaca acacaggtat ccatcccgaa agttattcta ttgcaaaaga aatattaact
    48001 tcagtccaac tttcagaaaa agagcttggt acagaagaag cagtagaaaa attgacacga
    48061 ttatccgtag agaaactggc tgaaagccta tcagtcggag aagaaacgtt agccgatatt
    48121 ttagctgggc tgacacaacc tggacgagat atgcgtgatg agatgccagc gcccttatta
    48181 cgtacagatg tattgagtat ggaagacttg aaaccaggga tggaattgac tggaacggtc
    48241 cgtaatgtca ttgacttcgg cgcatttgtt gatatcggtg tgaagcaaga cggacttgtc
    48301 catatatcga aactcagcaa aaaattcgtc aagcatccta cagatgtcgt gtcagtagga
    48361 gatatcgtaa cagtttggat agaacaagtc gatacgaaaa aaggcagaat cagtttgacg
    48421 atgctttccc cttatgaaga gtaacaatga ggagtaacaa aaattgacac aagaagaact
    48481 gcagcagtta gtagaagaga tatcattaaa aagcttccag cttcctttca agcaccaagc
    48541 aatgttcaat ccacgcttac gtacaactgg cgggcgctat catttaaacg atcatcattt
    48601 agattttaat gcgaagctat ttgaagagtc agacgaagag acgattgcag gaatcatcaa
    48661 acatgaactt tgccattatc atcttcatct agctggaaga gggtatcagc ataaagatat
    48721 ggatttcaag tatcttttga aaaaaacagg agggttgcgc tatgcaccag ccttgacgaa
    48781 gaaacagata aagatggagt attatcgatg ccaagattgt tctgccacga tctgccgcaa
    48841 gagaagaatc gatgtacgca aatatcgttg tggaagatgt catggagaaa tcaaatggat
    48901 tgagacaaaa agagaagact ttaatgaagc acaaaaaatc agatgaaagg atagaaggcc
    48961 tttggaaaaa agtgagcaga ggaatctaaa agactggaat cagctattaa attttttagc
    49021 cgcacctgaa gagggaaatt ttgaggaact gacggcacct acacttattt tagctggaaa
    49081 ctgtcttcct atcttagctg ataaagcagc acaaatgtat ttaaatggcc aagtagatca
    49141 attgtttttg gtcggagggg tggggcatgc gactagaatc ctttatgaga attttgaaaa
    49201 gcaaggattt cattttgaag atggcatgag tgaaagtgaa atctgtcgtc agtatctaaa
    49261 agaagtctat gatttgcccg ataaggcttt tctgatagaa tcgaagtcta caaattctgg
    49321 agagaacgcc actttttcac tggagattct tcattcctta ggagctgtac cagaaaaaat
    49381 cttattgatg aatgatccga cactgcaacg caggactcga gcaacttttg agaaagtatg
    49441 gcaaaatgaa caaactattt tcgcgaatgc tgtgccattt gtgccggaaa tccttcactt
    49501 ttctgaagag atcatcttta cagcaaaaga actgaatcat caatggccta aggaatattt
    49561 ccatgcgcta gtattaggag aaatggaacg tctccatgac gatgagaacg gctatggtcc
    49621 gaaaggaaaa gattttattc ctcacattga tatatcagag gaagtttggt cttcttacac
    49681 cagaattaaa gagtcaataa aaacggattt ttctcgtact tgaccatttt agcaagcgaa
    49741 tcctcctttc caaataaatt tggttgaaat tttcaatcgt ttataagata atgaggagcg
    49801 tgttgaatgt aatagctaaa agaatggacg ctagataaga gaagaggatt caaatggaaa
    49861 atcaaaataa attgtatcat tttgttggta tcaaaggttc aggaatgagc tccttggctc
    49921 ttgttttaca tgaacaaggt ctaaatgtcc aaggatcaga cattgaaaaa tatttcttta
    49981 cacaaagaga tttagaaaaa gcaaatatca cgattctgcc atttaacgca gataatgtaa
    50041 aaccagggat gacgatcatt gcaggaaatg catttcctga ttcacatgaa gaaatccagc
    50101 gagcaaaaga attaggtttg gaagtgattc gctatcacga ctttattggt cattttatcc
    50161 aaaattatac gagtatcgct gtaacaggct ctcacggaaa aacaagtacg actggactgc
    50221 tctctcatgt attgagtggt gtccgtccaa caagttatct aattggagat ggaacaggac
    50281 atggtgatcc gcaagcggaa ttcttttcat ttgaggcctg tgaatatcgc cgtcatttcc
    50341 ttgcctattc accagactat gcaatcatga ccaatatcga ttttgatcat ccggattatt
    50401 atacaagtat cgacgatgta tttacagctt tccaaacaat ggctggacaa gtgaaaaaag
    50461 ctatttttgc gtatggtgac gatgcttatt tacgcaaatt aaaagcaaat gtgccgattt
    50521 attattatgg tgtcacagaa aacgatgata tccaagcacg aaacatcgaa cgcacaactt
    50581 ctggttctgc ttttgatgtc tatcatgggg atgaatttgt tgggcacttc actgtaccag
    50641 cattcggtaa acacaatatc ttaaatgctt taggtgttat tgcagtagct tatttcgaaa
    50701 aactggatct gaaagaagtt gcagaagaaa tgctgacttt cccaggagta aaacgtcgat
    50761 tcagtgaaaa gatcgtagca gacatgaccg tagtcgatga ttatgcgcat caccctgcag
    50821 agatcaaagc aacgattgat ggcgcacgcc aaaaatatcc agataaagaa ataattgcag
    50881 ttttccaacc ccatacgttt acgcgtacga ttgcattgat ggatgaattt gcagaagcac
    50941 ttgatttagc tgataaagtt tacttatgcg atatttttgg ttcggctcgg gaagaacaag
    51001 gaaacgttaa gattgaagac cttggagcga aaatcaaaaa aggtggagaa gtcatcaaag
    51061 aaaataatgt atctccattg ttagattatc atgatgcagt agtaatcttc atgggtgctg
    51121 gagatgttca aaaatttgaa caagcgtatg aaaaactttt aagcagcacg acgaaaaatg
    51181 tactgtaata caattactgc agtgtcctta taaaaataga aagaggggga gtggcaaaaa
    51241 tcttgccaag cccccttttt tgcaacttat gataaacggt gttcaaaagc ttgcatctgc
    51301 ggtaataagc ccaatcaaat caaaaacatg agaagctgtt ttttcttggc tgttcttatt
    51361 atttgatgca ggacagcttt ttcacgacct ctgtaaacta taggaatctg ttaaatcaag
    51421 ggagcagaac ttgcctttta cgctactttt ggtacaataa tgtatgatta aaaataaact
    51481 ggagggtttc tatcttgaac ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa
    51541 tcgaggaagg cgactcgtta tccttaacag aatctattga agataaccag cttttgctgt
    51601 atttgggatt gacgaatgat gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa
    51661 cagaatatca acagccaatc gttccatcag tgatgctgat ggggatcatc acaagtgcaa
    51721 tttcaaagca tcttcctgga ccaggatcac atgttgtcaa tttttctgtc aattttattg
    51781 aaccggtttt ccattatgaa acactgactt ttcagtttga ggtcataaaa gtagataaga
    51841 tgaaggatgt ggtgacgatt tctgtagaag cggagaatac gaacgaagat cgtgtgttgg
    51901 atgctgtcgt tatggtccaa ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg
    51961 aaggtgaatt gaatgaataa tccaagtaca tcaatgtcag gtatcaatcg tttttatgca
    52021 aaagtttatg gatttttagg tatgggactt gcccttagtg cagtcacttc ttacttggct
    52081 ctttgggtat ttcctttgca attagcaact tttatcaata attttccact aggctttgtg
    52141 ggtctgtgga tcgttgaact gattttagta atggttctag gcgtaaaagc acaaaagaat
    52201 ccgagcttag caatcggtgg atttattgcc tactctcttt tgaatggcat aacattagcg
    52261 gttactcttg catactacga tattggaaca gtaacgcaag ctttcttgac agctactttg
    52321 atgttcatgg ggatgtctgt tgtcggagta acaacaaaaa gagatcttag cggggttgga
    52381 agagcaggac aggcagcttt aatcggagtg atcattgcac tagtcttgaa catcttcatt
    52441 ttgagaagta gcgtagttga gctgttcatc tccctactgt tagtcgtcat ctttgcaggg
    52501 attacagctt atgataacca aagcatccgt aatatctatg tccagacaaa cggaacagca
    52561 catagtggaa ttgctgtatt catggcattg aagctgtatt tggactttat caacttgttc
    52621 ttggcgtttc tgagaatctt tggacggaac aactaaaaaa gaaaaaaatt catcagttgg
    52681 ggctataccg aattatttgg tatggtcccg ctttttcttt tcgcattccg caggattctt
    52741 tgttagaatg aataagaact ttacagtaag gagttaagaa gatgacaaaa aataaattat
    52801 tactcgttga tgggaacagt gtggcttttc gcgcattctt tgcgcttcac aattctcttg
    52861 aacgtttcaa aaatagaaac ggtcttcata caaatgcaat ctatgcattc aatacgatgt
    52921 ttgaaaatgt tatgcaaaaa gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa
    52981 aaacgacttt tcggacagaa atgtatgcgg agtacaaagg tggaagatcc aaaacacctg
    53041 gcgaattcaa agaacaaatg ccatatatac gtgatttgtt gacaggacta ggggtacagt
    53101 attacgaatt gcccaattat gaagcagatg atattatcgg tactttggca gaaaaagtag
    53161 acaaagatca atttgatgtc gtcgtgcttt caggtgatcg ggatttgact caattagcta
    53221 gtaaagaagt aaaagtggat atcactgtaa aaggtgtcag cgatatcgaa agctatacac
    53281 cagaacatgt agcggaaaaa tatgatgggt tgacgccaaa acagattatt gacatgaagg
    53341 gactagccgg agatgcctct gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag
    53401 ctatcaagct attgaaacaa tatggctcag tcgaaggaat ttatgaacat atcgatgaaa
    53461 tgaagcaaag caaaatgaaa gaaaatctga taaatgacaa agagcaggct ttcttatcga
    53521 aaaaattggc gaccatcaat acttcagcgc ctgtggatgt ctctatcgac tcattgaagt
    53581 atgaagggaa aaatctcgat aaactcgttc cgttttataa agaaatggat ttcaatcagt
    53641 tcctttctaa attgaatatc gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag
    53701 tcgttcatga attcaaagag gaaatgttta ctacggatat ggcgttgtac gtggaaatga
    53761 tgggcgacaa ttatcataca gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa
    53821 tttatgtcac gaacgatcta tctgtattcg aaaaccgagc gttccattct tggatcactg
    53881 atccgactcg gctgaaaaaa gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct
    53941 atactggaaa aacgaaagga atcgattttg atgtcttgct aggtgcatac cttctcgata
    54001 cgaatgaaaa aagcacagat atcgaaggaa tagctgctca ttacggatac aacgatatcc
    54061 aatcagatga agctgtatac ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac
    54121 tcttttttgc tcatttagcg cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga
    54181 gccaagaact ggcagaaaaa aaccaagaag atcttttccg gaaaatggaa ctgccattgt
    54241 ctatcatttt ggcagagatg gaaataagcg ggatcaaagt agatgcgact cgcttacaag
    54301 aaatgaaggg cgagttctct gctcgcttaa gagagatcga acaaaaaatc tacgaagaag
    54361 ccggcgaaga attcaatttg aattcaccaa agcaattagg agtgatctta tttgaaaaaa
    54421 tgggattacc cgttatcaaa aaaacaaaaa ctggatattc cacagccgta gatgtactgg
    54481 aacagcttcg tgaacaagca ccaatcgtag aggatatcct cacctatcgt caaattgcaa
    54541 aaattcaatc tacctatgtc gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc
    54601 atacccgtta tgtccagaca ttgacccaaa caggacgatt aagctctgtt gatcctaatt
    54661 tgcagaacat cccgattcga ttagatgaag ggagaaagat acgtcaggca tttgttccta
    54721 gagaaaaaga ctggctgatt tattcttctg actattcaca aatcgagtta cgcgtacttg
    54781 cgcatatctc aaatgatgaa catttgaaag ctgccttcct agaaggtcaa gatatccatg
    54841 ccagcacagc gatgcgtgta tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc
    54901 gtaggcaggc aaaagcagtg aattttggta tcgtgtatgg catctctgat tacggacttt
    54961 cacaaaatct aggaatcagc cgaaaagcag cacagcaata catcgatacg tactttgaaa
    55021 aatacccagg tgtcaaagaa tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct
    55081 acgtcgaaac gctgtatcac cgccgtcgat atctgccaga catcaattca agaaattaca
    55141 atatccgttc ttttgcggaa cgaacagcta tcaatacacc aatccaagga agtgctgctg
    55201 atattttgaa aatcgcgatg atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag
    55261 ctacgatgct gttacaagtt catgatgaac ttgtcttcga ggtaccggaa aaagaacttg
    55321 aatcattaga taaattggtg aaggaagtta tggaacaagc tgtttctctt catgtaccac
    55381 tgatcactga cagcagttgg ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga
    55441 cagagcaata taatgaaggt gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt
    55501 tcgtaaagga ttggaacggt tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg
    55561 gcccaagatc atcgagcaac cagagacacc catctttgag gcttctcttg ttggcgaaac
    55621 catccaatcc attggacgta gagggaagtt tttaattttt catctcgatc attgtgaact
    55681 gatctcacac ttgcgaatgg aaggaaaata tcaatttacg aaagaaaata caccaatcga
    55741 taaacatacg catgtacttt tcttttttga ggatggaagc caacttcgct acaacgatgt
    55801 acgaaaattt ggtcgtatga caatcgtcga aaaaggagca agtgcaactt accgaggaat
    55861 catgaaattg ggacctgagc ctttgccaga tagcttctta ttagcagatt tcgcaaatgg
    55921 actgaaaaag tcccacaaag caatcaagcc attgctgcta gaccaacgtc tagtgactgg
    55981 tctaggtaat atttatgtag atgaagcatt atgggaagcg aagatccatc cagaacaacc
    56041 ggcgaacaca ctgcgcccca aagaggtaga acaattacgt ctatcgatca ttgatgtttt
    56101 ggatcgtgca atagaagccg gaggtaccac gattcgcagt tatttgaatg cattaggaga
    56161 atcaggcgga tttcaagtag cactgcatgt ctatcagcaa actggcaaac catgtatacg
    56221 ttgtggaacg ccgatcgtga aaacaaaagt ggcacaaagg gggacgcact attgtccgaa
    56281 ttgtcaaagg ctaaaaatcg caaagtagga tttgttttag gtttgacggg tggaatagct
    56341 accggtaaaa gtacggcagc caaagtcttt cagtctcatg gatttccttt gatcgatggc
    56401 gatgtgattg ccagagaaac tgttgagcct ggaacgcttg ctctgaggaa aattgtatca
    56461 gtgtttggcc aagaggtatt gcagcctgat gggcaactag atcgagggaa acttgggatg
    56521 attgtttttc catcaaagga aaaacggcaa aaacttgatc agctattaga tccgtttatc
    56581 cgaaaagcga taaaagatca aatcgcctct ctaagttcca atcatccttt agtaattgta
    56641 gatatcccat tgctctacga aggtcattat gaccactata tggatgcagt agctgtcgtt
    56701 tatactacgc cagaaactca gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa
    56761 caagcgcagc agagaattag tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat
    56821 attttgttcg acaacaatgg aacaaaagaa aaacttgtgg gacaaatcga aaattggctt
    56881 tttgctaatc gctttctttg agtatctgtg tcaattttgc tatcctgagt atatagaaag
    56941 tgagggatag cgatgggatc attaaaaaat caaaaggata aagtcgtagg taacgcaaaa
    57001 gaaaaaatcg gcgagatgct caataaagaa aatctaatcg atgaaggtcg tacgcaatca
    57061 gaaacagcag aagaaaaaga aaagcagtac caagcaagaa tcgatgaaat agaagaccag
    57121 caggcagaag aagaagcgaa gcggcaagat ttgactcaag aacgaaaaaa tccgacacct
    57181 gatcgtagta aaacaccgct cccagaaagt gaccacattc ccgatgagca aaaaatcgag
    57241 gaacagcgaa tgaaacacta tacaggaatc gaagagaagt tataaaatag taaaaaatcg
    57301 agatgtgact tgaagcagat gagaaggtgt tcatctgctt caagtttttt gtgctagatg
    57361 actaaatcag cttttgtgta agttatttct cagtaatcaa ggtgatcagt attggaaaat
    57421 tgaaatttag taaggacagg ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat
    57481 atccgtgttt tttgggtagg acagtcgcgt ttgtgatttg tttcgtgtta taatacaact
    57541 agttgtaaag aaagacacat cctttatata aaatgcgatg caagaggtga gacaaaatga
    57601 gatgtcccag atgtcaacat aataattcac gagtaatcga cagccgtcaa gcagatgatg
    57661 gacgtgcgat ccgtcgccgt cgcgaatgtg agaattgttc gttccgattc actacatttg
    57721 aacgaattga agctgctccg ttattagtaa tcaagaaaaa cggggaacgc gaagagttca
    57781 atcgcgataa aattttaaga ggactcatcc gttcagcaga aaaaagacct gtagctatgg
    57841 aacaaatgga acaaatcgtc aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg
    57901 aagtatcgac gaatctaatt ggtgaatatg tgatggaaga tttagttgat ttagacgaaa
    57961 tcgcttatat tcgttttgca agtgtttatc gccagttcaa agatatgtct gttttcttaa
    58021 aagagttgca agatatcgtg gataaagcaa aagaaaataa taaagagaat aataaagaaa
    58081 acaattaaga aacaaaaagg aggcatttcc ttggaaaatg cttggaaaga gcttcaacca
    58141 aaaaatattt accaaggaag aaaagcggct ccgctgagag aagacagcaa gaacgccttg
    58201 ctgtatcttt atcagccact aatcggcgga gaagccttga gtatctattt caccctgtta
    58261 tcagaaatct ctcaagaatc tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt
    58321 agtctaggct gcggtatccc gcaattttat gatgcaagaa aaaagctaga aggaatcggc
    58381 ttactggatg tttattttaa agaagatagt gatctgggac ctcgttttat ttatgagctg
    58441 ttagaaccac tcattcctaa agcatttttc aaagactctg ttctggcttt tcttcttctg
    58501 gaaaaagttg gtgaacgacg attccagaag cttacccaat tctttgaacc taaaaggcat
    58561 acagcagagg gatatcaaaa aatcacaaaa aaatttttag atgtttatcg cttcaatgaa
    58621 gcagtgtacg ctgctaatca agaaaacatc gaatcgctga ataaatcttt tgaagaatct
    58681 gaatcagtag cattacttga agcagattcg acattcgatt ggcagttttt gaatgattgg
    58741 ctggcaaaaa aacatattgc aagtcttgat gatccaatca tcaaacaagt cgacatgtat
    58801 catcagcttt acggtttcga tgaattgaca cttggcgaac tgatcagtca atcttatgac
    58861 tttagtgaac aaaaagtatc tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga
    58921 cgtccggtga agcaaacaaa agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg
    58981 tcaaacgaac atttgacaaa cgcagcgctt cagctgataa aagaagcaca gagcattcca
    59041 ccgatgaaat atctggaagc aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa
    59101 aactggttac tgcaagatct tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt
    59161 ttgctgaatt atgtactagt gatcaagaat aatgctagct taaatgccag ttatgtaaat
    59221 acagtggcaa atgaatgggc acaaaagaaa atcgccacag cagaagaagc gatccagcac
    59281 attcggcaaa taagtaatca agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa
    59341 actggcaagc gaaatgttcg acgggaaaag cttcctgatt gggtcaacca gccaaaagat
    59401 gaaaaacaga tcagcagcga gaaaaaagca gagattgacc gccgttttaa agaatacctg
    59461 gcaaaaaagg agggtgatat ctagtggaag acgtaggcaa agaattgaag aggatcatca
    59521 gcaaacgcaa ttatcaagaa aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg
    59581 atgttcaagc ttttctaagc gcacaccaag aagaattgac acaagcggat attgaaaaaa
    59641 gttacgctaa attgtatgaa ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa
    59701 cgatgatcgc tcccggctat gagccgaagc tgacgttgaa ttttcattat gtggatgtca
    59761 cttatgtacc aacggaagca ttgttagcac ggcaaagaca agaagaaatc agaagccgag
    59821 taaaagcaat ggatatgcca aaagatattc aatcggcttc ttttgaaaac tttgaacgta
    59881 cagatggacg aggatatgcg agcttagaag ccctagattt tgtagagaaa tatactgctg
    59941 atccaaaagg gtatcataaa ggactgtatc tagtaggaag ttttggtatt ggaaaaacat
    60001 atttattagg agcaacagcg agagaattag cgattgctgg attcaccacg acgctagtcc
    60061 attttccgac atttgccgtt gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa
    60121 agttagatgc agtgaaacgt tcacctattt tgatgatcga tgacattggt gcagatgcga
    60181 tgagtagttg gatacgagac gaagtattcg gtgttatcct tcaatatcgg atgcaggaac
    60241 agttaccaac cttcttctca tcgaatttta caatggatga attggaacag catttgtccg
    60301 tcactcaacg tggagatgaa gagccgctaa aagcgaaacg gataatggaa cgcatacgtt
    60361 atttgacaaa agaaatcgaa atgactggtc gcaaccgacg caatggttga acagttgaca
    60421 agtccaactg acatttgacc tagtagtaaa agcaggtcag cgaatcatta tatggacaga
    60481 aatgaccgga gacaatcacc gcaacaagta atgcagaaac ttaaacggag gattttttat
    60541 gagggctatt ttttctagta tatctgtagt tggtctattt tttcttttag caggctgcgg
    60601 tagcgcagat agcaacacag gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac
    60661 aagcagtacg aaaacggaga gacaaagcat atttgtcgga acgattgaaa gtatcactca
    60721 agtagaagca gaagaagaaa cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga
    60781 tccagcgaat atgggaacgt cattcagcaa tgacggagtg atcttaaatg ctactgctga
    60841 aaaaataagc gtggacataa atgaattgaa aatcggtgac aaagtccaat ttacattagt
    60901 tgagatgccg atcatgacga tgtctatccc accgcaagtt cctggagatt cgattattga
    60961 tgtttcattg aacaaataac caataacatt ttttcaaaaa taagaaaatg ttgttttata
    61021 gaacgaaaag gtatattttt taaggagata tgggaatttt tttattccta gtctccttat
    61081 tttatttgga aaatgctata attatctaga aggagtgaag ctatgaacca tttagattta
    61141 tctcagcgct tagccgcagt gggtgacttt gtccctgaat ccgctcgttt agcagacatt
    61201 ggatcagatc acgcttactt acccgttgca ttgatattaa gaggaaaaat cgaatatgca
    61261 gttgctggtg aagtcgttca aggtccttat gaatcagcac gtaagcaagt caaaaaagat
    61321 ggattagaag accgtatcac cgtacgcctt gcaaatggtc tagatgctat ccagccacaa
    61381 gatggtatta cagctgtcac gattgcgggt atgggaggtt cactgatccg agatatccta
    61441 gaagctggca agttgaatca acgcttaagc ggacaagaac gtttgatcct tcagccaaac
    61501 atcggagagc gtacattacg atcttggctg caagaaaatc agtataaaat ccaatcagaa
    61561 aaaatacttg aagaaaataa aaaaatatat gaaatcattg tagccgaaaa agaacaaaaa
    61621 ccagttactt acaatgaaat ggagctgatg ttcgggccat ttctgcttga agagaaaagt
    61681 gagatcttca aatcaaaatg gacaagagag cttcaacaaa gaaaagctat ccgtgaacag
    61741 ctcaaacaag cagctaatca agaggcacgt caaaaacaaa tcgagcagga aatccaaatg
    61801 attgaggagg ttttaaagcc atgacaatca gcggacgcac tttcatccaa aagtttgaaa
    61861 gctattgtcc tcagtggcta gctgaagaag gagacccagt cggactgcat atcggaacat
    61921 tggataagcc aatccaacga gtaatgatga cattagacgt tcgtccagaa gtggtcgaag
    61981 aagcaatcaa gaaaaaaatc gatttgctga ttgctaaaca tcctccaatc tttcgtcctg
    62041 taaaacggct ggttactgat cagccacaag aaaaaatgta tgcagatttg ttgaaacatg
    62101 atattgccgt ctatgcagca cacacgaaca tggatattat ttgggatgga ctgaatgact
    62161 ggttttgtga acttctcggt attgaagtgg agagttattt agtaaagact catgagattc
    62221 attataaaaa actggccgtt tatgtacctg tggatcatgc acagaaaatg cgtgaagtat
    62281 tagctgcagc tggtgctggt acacaaggtg attataccgg tacaagtttt acctcgattg
    62341 gacacggtcg atttactccg gaagctggtg cacaaccagc gattgggaaa gtagggaaga
    62401 cagaacaagt tcaagaggca aaagttgagg ttattttgcc ggaaactatc gaaaaacaag
    62461 tgattcaagc catgcggtca gctcatcctt atgaggaacc tgcttatgat cttttcgcta
    62521 tagatgaacc agtcgaaatg tttgggttag gacgagtagg tgaattgccg caagaaattt
    62581 ctatagaggc atttgtagaa caagtaaaag aagcgttcca actagatgga ctgagaatag
    62641 tccagccaaa aaatgcaaaa tcctctgtga agcgcattgc gatttgcggt ggaagcggtg
    62701 aaaaatttta tccgcaagca atcgcccaaa gagcagatgt ctatatcacg ggagatatct
    62761 attatcatac ggctcatgat atgcaaagcg ctgggttgat tgcaattgat cccggtcatt
    62821 atattgaatc gttatgcaag caacgattta tcgagaaatt tgaatcttgg aaacaagaag
    62881 aaaattggga tattaacttt ttcgtttctg aaacaaatac aaacccattt caatttaatt
    62941 aaaaggagag aatactatgt acgaaaatct tttgccacgt tttttacgat atgtcaaaac
    63001 agaaacacgc tccgatgcaa caagcaaaac gactccatcg actcagacac aagttgcttt
    63061 tgcgcaagaa ttgaaaaaag aattagaaga gttaggctta gctgatgttt tctataatga
    63121 agataacgga ttcgttatgg gaacattacc aagcaatgtt gatcatgaag tgcgttccat
    63181 tggatttatt tcccacatgg atacagcaga ttttaatgca gtaggagtca atccacaaat
    63241 cattgaaaac tatgatggcg agtctacgat cgtactagat gcggaagaac gctatacatt
    63301 gaatacaaaa gattttccaa atttgaaaaa ttatgaaggc cacacattga tcacaacaga
    63361 tggcagtaca ttactgggag cagatgacaa atcagggatt gcggaaatca tgactgcaat
    63421 ggagatcttg atcaataatc cttctatccc tcacggtacc atcaaagtgg cttttggtcc
    63481 tgatgaagaa atcggtgtcg gagcggataa attcgatgtt gagcaattcg atgtagattt
    63541 tgcttataca gtcgatggtg gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc
    63601 tcaagcaaat attacgatcc aagggaaaaa tgtacatcca gggacggcaa aagatacaat
    63661 gatcaatgct ctacagttag caatcgattt tcataatcag cttccagccg atgaagttcc
    63721 agaaaaaacg gaaggatacg aaggtttctt ccatttgatg gcattggatg ggaatcctga
    63781 agaagcttct atgagttata tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa
    63841 agcacaaatc acacatatcc aagaagagtt gaatcagcga tttgatcaag aacgaatcaa
    63901 agtagatatg tatgatcaat attacaacat gaaagaaatc attgaaaaag atatgagcat
    63961 cgtggaatta gcgaaacaag caatgatcga gcttggtatc tctccagtga tcgaaccagt
    64021 tcgtggagga acagatggtt caaaaatctc ttacatgggt attccgacgc caaatctatt
    64081 tgccggagga gaaaatatgc acggacgatt cgaatttgtt tctttacaag taatggaaaa
    64141 agcaacagac gtgattgtaa aaattgcaga attgaacaca aagagttaaa atcgaaaaag
    64201 aacagcttat gacaaggcgt tcgtcccaat gatggaatgt catctgtcta aggctaaagg
    64261 gcaggacaat ggaattggtt gtcccgccct cttttttgaa gagtaaatag gaggtaaaga
    64321 atggaaaagc tgctagtttt ttataacaaa agttcaggga aagatgaggg tgagaaatta
    64381 gcacgctggt ttcaagatta tgtaaaagaa aagcagccag aattatatgt agaactagct
    64441 gaaactggcc catcgattga tgatgcggaa ttagtcaaga aagcagaagc catagaagct
    64501 gatactttag taattatagg tggagacggc acgattcatc atatcgtcca agcgttccaa
    64561 aaaacattat ctcaatacca tgtcggatta cttccaggtg gaactgtcaa caaccttgca
    64621 cgtgttcttg agattcccct caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat
    64681 gtacggaaaa tagactatgg tcaagtcaat gatgaagtca ttatctctac actgacaatc
    64741 ggaatcttag cagatacagc agtttggatc agtcaagaag aaaaacagcg ttacggctcg
    64801 tggatcttta tcaagaagtt ttttaagtta ttgctgaaga agaaaaaata tcatttggat
    64861 ttagaaacag acgaggagca ttggaaagga aaatcccaac tgttgacagt cacgatgtct
    64921 aactccgtgg gcggttttac aaattttgac gactcagcga ccctagatga tggaaaattt
    64981 catgtaacga tcgtaccttc cttagatatt ttccgattta ttttttattt gcctagaatc
    65041 atgagaggca aattctatac gatccccggc attacttatt tcacagccaa acgcatgaaa
    65101 atccaagcgc aggaaaaatc tgtgggcact cgaacggatg gagacacgac agatgatctt
    65161 ccagttgaat tatctgttgt atcaaaagga ttaacagttt attcaccaaa gaaagaagaa
    65221 aaagcttaga aatagtagtt aacagaaagt tggaagaaga gtaatggatg tattgttaca
    65281 caatcctttg tggttttacc ttttcattgt gaatttttac ttattttgtc tgatggggta
    65341 tgataaacat caggctagaa ctagcggctg gcgtgttcct gagaataatt tgatatttgt
    65401 cagcctgtta ggcggtggtt tgggaggatt gatctctcga aaagttttcc atcataaaac
    65461 tcggaaaaag aagtttacta taggattcgc tgcaggtgta cttgttgatt tgattctcat
    65521 ttattttttc cattaaggag caggagatgt cgtctaaagg aacgaaaact aaaatcattc
    65581 tgaatctgct tttgctgatt gtgatttttg ccattattta ttatttaatc aatcaatcat
    65641 ttgcagatat ctttgccgaa ttgctgtcta ccagtgttgt tgttttggca gcggctgtta
    65701 tattagggac agtttaccaa atcgctgaag ggcgctcaat caaagaaatt gctgaacctt
    65761 tcgcgccgaa ttttacaacg aaagatgggt tcttgacctc tagctatatt gcgttttacc
    65821 gaatcgtttc gtttggtaca ggaacgctga tctcggagat ttatttttat aacaaaaaag
    65881 gaatgaagct atcccaggga gcaggggtga cagcactcca tatgattatg tacaaactgg
    65941 cagtgatcac ttatgcatta gctggcttgg tcatccaatt ctctcttttt tattctaatg
    66001 gtccatcaat gatctggttt atcatcgctg gtatcctttt gacgatcgtg attgttgcct
    66061 ttttattggc gatgtccgtc agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata
    66121 agttatttaa aagtgagcgt ttgcggaaat gggtggatac atgcaatcag cagatctact
    66181 ctttaaggga aacggtccaa accattatcc aagacaggac tgcgctattg cggatttatc
    66241 tatggaatat gttaaaactt gtattttggt atgtacttcc ttatttgatt ctgattgaaa
    66301 accatcctaa aatagatttt ctactgacca tttcgtttat cagctttgca gtagtgcttt
    66361 caggggttat tcctacgcct gctggtattg gttcctttga atttgtctat ttgttattat
    66421 tcagaccagt tgttggaaca gttgacgcag tttcttcgct tttactgtat cgtttcagtt
    66481 cctttatctt gccatttctc tatggatttg tgtatgtgat tgctgaacgg cgaagagtga
    66541 tcaaagaaga aataatagaa gtaaaaaaag agaataaaga aaatcaaggg taggtctttt
    66601 ataagatcta ccctttttat caaatcaaag aaaaacgtta tggataagat aattttacgt
    66661 ttgtttggct ctttttttat atacatacaa tcaattttgt ttttttaaga tttatataag
    66721 tcgagaaaaa aagtcaaaaa actatgtgta tattttgacc aaaattgacc aaagcgatat
    66781 aatagttttt gtaaagatca aagaaataag tgatcttacc tttaattagg agggtatacc
    66841 tgtatgaata ttgagaaaat gactacgacg cttcaagaag cgattgctga agctcaacag
    66901 attgcggtca ctcgaaaaca tcaagatatc gacattgccc atgtatggaa aatcttttta
    66961 caatctaatc attttggtcg aaatttctat acggatgccg gattggatgt cgaatctttt
    67021 gaacatgaaa ttgatcgtct actggatgag tatccagttg tttctggtgg aaacgtccaa
    67081 tatggacaaa acctcagcca gaatttcttt cgattattga atgaagcaga ccaaatacgt
    67141 gaaagcttcg gcgacgagtt tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta
    67201 aaaaattatc cgttaactgt ttatttgaat aaaaacggat tatctgaaaa agaattgcgt
    67261 aaaaacatcg aagagatgag aggaggagaa agagtgacct cacaaaacca agaagaacaa
    67321 tacaaagcat tagaaaaata cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg
    67381 gatccaatca tcggtcgtga tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa
    67441 acaaaaaata atcctgttct gataggagaa cccggtgtag ggaaaacggc aatcgttgaa
    67501 gggcttgctc aacgtatcgt acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt
    67561 ttttcattag atatgggtgc attgatcgct ggagcaaaat tccgtggtga atttgaagaa
    67621 agactaaaag cagtcttgaa agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc
    67681 gatgaaatcc acaatatcgt aggggcagga aaaacagaag gaagtatgga tgccggaaat
    67741 ctgttgaaac caatgcttgc tcgcggtgaa ttacatttga tcggtgccac aactttagat
    67801 gagtaccgtc aatatatgga aaaagacaaa gcgttggaac gtcgctttca aaaggtatta
    67861 gtcaaagaac caacagtgga agatacgatc agtattctgc gtggattaaa agaacgtttt
    67921 gagatccacc atggtgttaa catccatgac aacgcattag tagcagcagc tactttatct
    67981 gaccgttata ttactgatcg gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc
    68041 agtgcaacga ttcgggtaga aatgaactct atgccaacgg aattagatca agttactaga
    68101 cgtttgatgc agttggaaat cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt
    68161 aaaaaacgct tgaaaaattt gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg
    68221 atgaagatgc agtgggaaac agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct
    68281 gaaatcgata aagcaaaaca cgaattagaa gatgcagaaa ataactatga tttagaacgc
    68341 gctgctgtat tgcgtcatgg tacgatacct caattagaaa aagaattgaa ggaattagaa
    68401 gctaaagcaa aagacagcga gattaagatg gttcaagagt ccgttaccga aaatgagatt
    68461 gctcaagtcg ttggcagatt aacgggtatc cccgtaacga aacttgtaga aggtgaacga
    68521 gaaaaactaa ttaaattgaa tgaaacactt cataaacgag tgattggtca agatgaagca
    68581 gttgatgcgg tcagtgacgc ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt
    68641 cctctaggtt ctttcctttt cctaggacca actggtgtcg gaaaaaccga attagcgaaa
    68701 gcgttagctg agaacttgtt tgactcagaa gatcatatgg tacggatcga tatgagtgaa
    68761 tatatggaaa aacatgctgt gtctcgtcta gtcggagctc ctccaggcta tgtaggttac
    68821 gaagaaggtg gtcaattaac ggaagctgtc agacgaaatc cttatacaat cgtcttgttg
    68881 gatgaaatcg aaaaagcgca tcccgatgtc ttcaatatct tgttacaagt cctagatgac
    68941 ggacgcttga ctgattcaaa agggcgagta gtagatttca aaaatacggt tctgatcatg
    69001 accagcaata tcggttcaca gttactgctg gaaggcgtga ctgctgatgg aacgattcca
    69061 gaagcagtgg ctgaacaagt caacacgcta ttacgtggaa acttcaaacc agaattcttg
    69121 aaccgaatcg acgacacgat tttatttaca ccattgagcc tagacaacgt gaaaggaatc
    69181 gttgacaaaa tggtggcgca attggcacaa cgtctggaac atcaagaaat cctgctgaca
    69241 atcagtgatg aagccaaaac gtggattgct gaaaatgctt atgaaccagc atatggtgca
    69301 agaccattga aacgatttat cactaaggaa gtagaaacac cactagctaa agaaatcgtt
    69361 gcaggacatg ttatgcctaa atcaaaagta acgattactt tacttgatgg acaacttcat
    69421 ttcaaaacag aagagctaga agaaatcgtc taatcttcca ttgagactat aaaagaaaaa
    69481 aggggtctga cacaactagt gtcagacctc cttttttcaa ataaacagtg ttccagaagt
    69541 agtttcttcg aaaaataagc cgaaatttct tgaagctgcc cgcttctgtc acaacctctt
    69601 ttattaggta tttttcactt tttctaattt ataactttca aagtaacgct catctttttt
    69661 actttgagac catgcaaaca atcccattaa ggcagcacct atcgtatttg tcagcaagtc
    69721 gcccatcgta tccatcaatg ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa
    69781 acgttgaaga ttcatgccta agaactggtc gcataagaat tcccagaact cccaaaacac
    69841 gccgcaaagt ccagcaaacg caaagccgaa taagaggaac aaccaaggac tggtttttga
    69901 tatctcagca tctttcatca agtaaccaat caatccatag ccaagagctg tcaggaccat
    69961 tggactcacc gcatgtaaga ttttgtccca aaaagagatg atactgatca aatgcatccc
    70021 tgtgcctaaa aagacagaga tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg
    70081 tattttaagt ttgaaaactt tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa
    70141 taaagattcc acgataaatg tgattccttg agaagtcgaa tatttttgtg tgataaattc
    70201 ataaaaatta tagcagacaa cgaatacacc aaaagcaagt aaagctattt gcaccttttt
    70261 gatttcttta atcactgaaa gtccctccat ctaaaaatct ttttacttct gaaagcaact
    70321 caatttctga agaaaccact tttccgttca atttgatcaa acctattgta tataagttca
    70381 ggtagtgaaa ttgattttca gctgtttctt gtagtgcagc gatttttttc gggtgggttc
    70441 cgccttgctg tcgagtatca gtatataagc cgatcactgg gatgcctttc gcataagcca
    70501 caccgatttc cgatgctacc ccggcatcga tcgtcagccc atctagtaaa gcaatcatta
    70561 aatcactagc caacactttt tctgtgtcag caagagcaat cattcgactg tctgcataag
    70621 cagttttgtc attgattgct tcattttctt gaggaagata gatcgaaagc tcagaagaga
    70681 gctctctgat ttctttaact aactgttggt tatataacaa atcactttta gcaaacattg
    70741 gtgcggcaaa ataaattttc atctaaacat catccttttt atcaaaaata atcgctaaag
    70801 taagcttcca tgtttggaat gatacgctct agtgacttca gtgtttcttc atctgtttct
    70861 aaccgttcga ttcttgccaa ataagaagca cggcgataat tcatcatcaa gcaagtcaat
    70921 gtttggatat ccagtcgaac ggattttcct ataggttcat ttaagacacg gacttgtccc
    70981 tgttcatccc atgttaaagc gaagacacca ttattccaag gagcgacagg atcttcaatg
    71041 atgaaatgaa aagcatcagc tgttccaaca aacggaaacc gttgaaggaa ttcttttaca
    71101 tcaacgatcc gagccataaa atatggttcg atctgctctt tgatctgact atcttcaatc
    71161 aaaaatgcta aaggctcatt tttataaata tctccatgta cccagtagat catggaaaaa
    71221 tgtgcggaaa taaagttcca taagccattt cgagcttctt gatcaagata gaacatttct
    71281 tttacgtgaa atatttcttc agctacccag tagaacagaa cacctcttgg ttcatggttg
    71341 ctatcgtagt agacagcggc tgtgcgctca tcttcatttt cgaaacgcca atactcttcc
    71401 caatgaaaat cactgcggaa taaagcacca tggttttgtt gggcaaattt gtcataaacg
    71461 gaaaatacgt ctggatgatc aactggtaat cgctcaacca taccacttac tgggatttgt
    71521 ttaggtagct gtgtatctct gattttaaag gaaagtttat cagacatgat ttcccatcct
    71581 ttacgccgat agtaggggat actgtaagga tacaaataag agatccattg cttattcttg
    71641 cgcatattat ccaatgcaac gataattaga tcttgcatca atccatggtt agcatattct
    71701 ggataagttc caactccagt cacaccaccc attttataac gtgcaccgtg aatattgact
    71761 tcacaaggat agatagcgat ttgagagatc agtttatttt cgtggaacca tccgaacact
    71821 ttcgatactt ccaaaatcgg ttttttggaa cggataaaag ctcgtttatt ctcaaacccg
    71881 ctttcctcga tgtctgcttc agtcacttgg aacacatagg aaagcaattc attgaattga
    71941 tcgatatgtt cttgagtgac cggttttaat tcaagatttt ctctaaaatc tttttgatcc
    72001 attttttcat ctccatttat tagcttgata acagtatagc aaaattctac aaaaaaaact
    72061 tctatagctg actaattaaa aactctttaa attactgctt ttcgttggta taatggaaag
    72121 tgctgattat atgaaaagag gagacgctat ggatataaag aaaatgaaag aacgccaaga
    72181 gcagattcgt aatttttcga tcatcgctca tatcgaccat gggaaatcta cattggccga
    72241 tcgtatttta gaaatgacgc acactgtgac gtctcgcgaa atgcaagatc agctgttaga
    72301 ctcgatggat ttggaacgtg aacgtggaat cacgattaaa ttaaatgctg tagaactcaa
    72361 ttacacagcg aaaaatggag aaacatatat ctttcacttg attgacaccc cagggcacgt
    72421 cgacttcacc tatgaagtct ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt
    72481 cgatgcagca caaggaatcg aagcacaaac attggccaat gtttatttag cgttagacaa
    72541 tgatttggag atcctgccag tcatcaataa aatcgaccta cccgcagcag atcccgaacg
    72601 tgtccgtcaa gaaatcgaag acgtaatcgg aatcgatgct agtgaagctg tattagccag
    72661 tgccaaatca gggattggga ttgaagatat tctcgagcag atcgtggaat atgttcctgc
    72721 accaagtgga gacttagatg caccgctaaa agctttgatc tttgactcta tttatgatag
    72781 ttaccgcggt gttgttctaa atgtccggat cacggacggt atggtcaaac ccggagacaa
    72841 aatcaaactg atgagtaatg gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc
    72901 aaaagcagtt gctagagact ttttgatggt cggtgatgtc ggttatatca cagcaagtat
    72961 caaaactgtc caagatacac gagtagggga tacagtaacg ttagcagaca atcctgccgc
    73021 tgaagcttta ccaggttacc gaaaaatgaa tccgatggtt tattgtggtc tttatccaat
    73081 tgatacttct cgttacaacg atttgcggga agctttagaa aaactgcaat tgaatgatgc
    73141 tgctttacaa ttcgaaccag aaacctctca agcattaggt tttgggttcc gctgtggatt
    73201 cctcggactt cttcatatgg atgtcgtcca agaacgcttg gaacgggaat tcaatctaga
    73261 attgatcacg actgcaccat ccgttattta ccatgttaat aaaacggatg gtacgactgc
    73321 tacagtagat aacccggcag attttcctga acctgtaacg attcaggatg tagaagaacc
    73381 attcgtcaaa gcacagatca tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc
    73441 tcaacgaaaa cggggagaat tcattacgat ggattattta gatgattacc gagtaaatgt
    73501 cgtctataat attccgcttt ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag
    73561 cacgaaaggc tatgcctcat tggattatga aatgtctggt tatcaaaaaa gcaagttagt
    73621 gaagatggac atcttgctga atggagaaaa agtcgatgca ttaagcttta tcgttcaccg
    73681 agactttgcc tatgaacgtg gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg
    73741 ccaacaattt gaggtgccaa tccaagcagc tattggacaa aaaatcgttg cccgttcaga
    73801 catcaaagcc ttgcgtaaaa acgtcttggc taaatgttat ggtggagatg tttctcgtaa
    73861 acgtaaactc ttagagaaac aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt
    73921 cgaagttccg caagaagcct tcatggccgt tctgaaaatg gacgaggacg aaccgaagaa
    73981 atagcaaggg tttgtttgaa aacagtgtaa aaaataaacc atgtttacat ggatttgcca
    74041 ccggtttgcc accggtggtt ttt
//
LOCUS       DACCXM010000002        60326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCXM010000002 DACCXM010000000
VERSION     DACCXM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.002"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1421)
                     /locus_tag="IVY61_000069"
     CDS             complement(<1..1421)
                     /locus_tag="IVY61_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7969213.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1673..2854
                     /locus_tag="IVY61_000070"
     CDS             1673..2854
                     /locus_tag="IVY61_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7969214.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2914..4542)
                     /gene="treC"
                     /locus_tag="IVY61_000071"
     CDS             complement(2914..4542)
                     /gene="treC"
                     /locus_tag="IVY61_000071"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP7969215.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4993..7704)
                     /locus_tag="IVY61_000072"
     CDS             complement(4993..7704)
                     /locus_tag="IVY61_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7969216.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7714..8415)
                     /locus_tag="IVY61_000073"
     CDS             complement(7714..8415)
                     /locus_tag="IVY61_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969217.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8601..9200
                     /locus_tag="IVY61_000074"
     CDS             8601..9200
                     /locus_tag="IVY61_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7969218.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9281..10294
                     /locus_tag="IVY61_000075"
     CDS             9281..10294
                     /locus_tag="IVY61_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7969219.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10402..11319
                     /gene="rbsK"
                     /locus_tag="IVY61_000076"
     CDS             10402..11319
                     /gene="rbsK"
                     /locus_tag="IVY61_000076"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP7969220.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11347..11742
                     /gene="rbsD"
                     /locus_tag="IVY61_000077"
     CDS             11347..11742
                     /gene="rbsD"
                     /locus_tag="IVY61_000077"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP7969221.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11742..13115
                     /locus_tag="IVY61_000078"
     CDS             11742..13115
                     /locus_tag="IVY61_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7969222.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13472..15616
                     /locus_tag="IVY61_000079"
     CDS             13472..15616
                     /locus_tag="IVY61_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7969223.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15668..16708)
                     /locus_tag="IVY61_000080"
     CDS             complement(15668..16708)
                     /locus_tag="IVY61_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7969224.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16985..18787)
                     /locus_tag="IVY61_000081"
     CDS             complement(16985..18787)
                     /locus_tag="IVY61_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7969225.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18801..19553)
                     /locus_tag="IVY61_000082"
     CDS             complement(18801..19553)
                     /locus_tag="IVY61_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969226.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19562..19924)
                     /locus_tag="IVY61_000083"
     CDS             complement(19562..19924)
                     /locus_tag="IVY61_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7969227.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20100..20972)
                     /locus_tag="IVY61_000084"
     CDS             complement(20100..20972)
                     /locus_tag="IVY61_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP7969228.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21023..21511)
                     /locus_tag="IVY61_000085"
     CDS             complement(21023..21511)
                     /locus_tag="IVY61_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7969229.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21649..22479)
                     /locus_tag="IVY61_000086"
     CDS             complement(21649..22479)
                     /locus_tag="IVY61_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP7969230.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(22495..23103)
                     /locus_tag="IVY61_000087"
     CDS             complement(22495..23103)
                     /locus_tag="IVY61_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP7969231.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23096..23413)
                     /locus_tag="IVY61_000088"
     CDS             complement(23096..23413)
                     /locus_tag="IVY61_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7969232.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23569..24297)
                     /locus_tag="IVY61_000089"
     CDS             complement(23569..24297)
                     /locus_tag="IVY61_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP7969233.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24456..25337
                     /locus_tag="IVY61_000090"
     CDS             24456..25337
                     /locus_tag="IVY61_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7969234.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25574..26617)
                     /locus_tag="IVY61_000091"
     CDS             complement(25574..26617)
                     /locus_tag="IVY61_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7969235.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26620..27288)
                     /locus_tag="IVY61_000092"
     CDS             complement(26620..27288)
                     /locus_tag="IVY61_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7969236.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27430..29766
                     /locus_tag="IVY61_000093"
     CDS             27430..29766
                     /locus_tag="IVY61_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7969237.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29881..30192)
                     /locus_tag="IVY61_000094"
     CDS             complement(29881..30192)
                     /locus_tag="IVY61_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969238.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30373..31848
                     /locus_tag="IVY61_000095"
     CDS             30373..31848
                     /locus_tag="IVY61_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP7969239.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31895..32479
                     /locus_tag="IVY61_000096"
     CDS             31895..32479
                     /locus_tag="IVY61_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP7969240.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32481..32864
                     /locus_tag="IVY61_000097"
     CDS             32481..32864
                     /locus_tag="IVY61_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP7969241.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33181..33465)
                     /locus_tag="IVY61_000098"
     CDS             complement(33181..33465)
                     /locus_tag="IVY61_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP7969242.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33817..34527)
                     /locus_tag="IVY61_000099"
     CDS             complement(33817..34527)
                     /locus_tag="IVY61_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP7969243.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34524..35996)
                     /locus_tag="IVY61_000100"
     CDS             complement(34524..35996)
                     /locus_tag="IVY61_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7969244.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36134..36607
                     /locus_tag="IVY61_000101"
     CDS             36134..36607
                     /locus_tag="IVY61_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7969245.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36760..37311)
                     /locus_tag="IVY61_000102"
     CDS             complement(36760..37311)
                     /locus_tag="IVY61_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7969246.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37416..37946
                     /locus_tag="IVY61_000103"
     CDS             37416..37946
                     /locus_tag="IVY61_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP7969247.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38429..38872)
                     /locus_tag="IVY61_000104"
     CDS             complement(38429..38872)
                     /locus_tag="IVY61_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7969248.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38888..40048)
                     /locus_tag="IVY61_000105"
     CDS             complement(38888..40048)
                     /locus_tag="IVY61_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7969249.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40555..42255
                     /locus_tag="IVY61_000106"
     CDS             40555..42255
                     /locus_tag="IVY61_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969250.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42357..43190)
                     /locus_tag="IVY61_000107"
     CDS             complement(42357..43190)
                     /locus_tag="IVY61_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP7969251.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43494..45659)
                     /locus_tag="IVY61_000108"
     CDS             complement(43494..45659)
                     /locus_tag="IVY61_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7969252.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45919..48213
                     /locus_tag="IVY61_000109"
     CDS             45919..48213
                     /locus_tag="IVY61_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7969253.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48206..48871
                     /gene="pgmB"
                     /locus_tag="IVY61_000110"
     CDS             48206..48871
                     /gene="pgmB"
                     /locus_tag="IVY61_000110"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7969254.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48864..49871
                     /locus_tag="IVY61_000111"
     CDS             48864..49871
                     /locus_tag="IVY61_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7969255.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49893..50912
                     /locus_tag="IVY61_000112"
     CDS             49893..50912
                     /locus_tag="IVY61_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7969256.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="IVY61_000113"
     CDS             complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="IVY61_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7969257.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52669..54090)
                     /locus_tag="IVY61_000114"
     CDS             complement(52669..54090)
                     /locus_tag="IVY61_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7969258.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="IVY61_000115"
     CDS             complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="IVY61_000115"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7969259.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56501..57757)
                     /locus_tag="IVY61_000116"
     CDS             complement(56501..57757)
                     /locus_tag="IVY61_000116"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP7969260.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            58337..59638
                     /locus_tag="IVY61_000117"
     CDS             58337..59638
                     /locus_tag="IVY61_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7969261.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59707..60306)
                     /locus_tag="IVY61_000118"
     CDS             complement(59707..60306)
                     /locus_tag="IVY61_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7969262.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18208 a        12348 c        10817 g        18953 t
ORIGIN      
        1 tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg atcactgaat
       61 tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata ccttacgcca
      121 cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt tgaacgtttc
      181 gttgttccat tcttaaacgt aaatgccatc tctttctcac tgatcttgat tgaagcaata
      241 tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt atcctgattc
      301 aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata cgtatctact
      361 gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc gtcttcaaaa
      421 taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag cgggttttct
      481 gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag atagttttct
      541 ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc taaagataca
      601 cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc aaaataaata
      661 tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc cgcagacggt
      721 tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc taacgctaga
      781 tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt aaaggtttct
      841 tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt ttttgtccat
      901 acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc tacctgcttg
      961 attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg gccttcttcc
     1021 ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag cataccttct
     1081 gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc ggggacccat
     1141 gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt tgccatgtct
     1201 ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa ctctacttct
     1261 ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt cgtcatcacg
     1321 ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt taacatacct
     1381 gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac tcctcttctc
     1441 atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt tacgttttag
     1501 caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct tattttaatt
     1561 gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct atcattttcc
     1621 ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa gagtgaaaaa
     1681 agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct cttactatgt
     1741 tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga tctcgtttat
     1801 attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt ttgcactgat
     1861 cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt cgaggaactt
     1921 gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa gcatatggct
     1981 tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag atttgtttca
     2041 agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg attcatttaa
     2101 tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta ttggaaatta
     2161 ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag ctatttttat
     2221 tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc aaaaatttta
     2281 tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa atttgacaaa
     2341 gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt catttatcct
     2401 tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta gtgttttagc
     2461 tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg ccttctttcc
     2521 aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg tcattgtctg
     2581 gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg gtgatcgttt
     2641 acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc taatgggaat
     2701 tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg ttacttttat
     2761 tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag agtatgaaaa
     2821 ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat cttacgagaa
     2881 tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag caattgcctg
     2941 gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat cgataagaat
     3001 attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat agaaatttgt
     3061 taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt ccggatgatt
     3121 tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc tcagccgaat
     3181 caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt ctacattaat
     3241 ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc ctgcttgttc
     3301 tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt tgatccgacg
     3361 aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat tcaaactttc
     3421 tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga tttcttctcc
     3481 catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca tctctgctcc
     3541 ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct gatcatgatt
     3601 attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc atgtatgaaa
     3661 tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc cattttcata
     3721 atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac taggttctgt
     3781 atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga tgatagtgga
     3841 atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga ttggtttgtc
     3901 cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt ctttaccgat
     3961 aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa aacgaacaac
     4021 tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt gtgtccgatc
     4081 atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac ttccaccaaa
     4141 ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat aatgctggtc
     4201 ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca gcaccatatc
     4261 gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga attcctccat
     4321 cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt tgtctttttg
     4381 agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat tcagatacgg
     4441 gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt ctttgtaaga
     4501 tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt acctccattc
     4561 aactgattta ttacctacca caacttatat atacaagtta tcatttatta ttataactga
     4621 accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg cccaataaaa
     4681 aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa atattcttgt
     4741 ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc tcaaatgtaa
     4801 aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac ggctttttta
     4861 caacctctga ttcatggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa
     4921 aatatgaaaa actattttgc cgtttgtttc tctaatactc aggtcaaacc gctgatttca
     4981 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5041 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5101 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5161 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5221 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5281 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5341 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5401 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5461 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5521 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5581 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5641 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5701 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5761 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5821 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5881 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     5941 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6001 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6061 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6121 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6181 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6241 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6301 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6361 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6421 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6481 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6541 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6601 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6661 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6721 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6781 ttgagataat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6841 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6901 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     6961 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7021 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7081 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7141 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7201 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7261 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7321 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7381 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7441 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7501 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7561 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7621 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7681 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7741 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7801 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7861 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7921 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     7981 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8041 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8101 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8161 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8221 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8281 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8341 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8401 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8461 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8521 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8581 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8641 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8701 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8761 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8821 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8881 aggattcttt gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt
     8941 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9001 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9061 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9121 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9181 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9241 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9301 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9361 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9421 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9481 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9541 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9601 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9661 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9721 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9781 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9841 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9901 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
     9961 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10021 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10081 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10141 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10201 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10261 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10321 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10381 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10441 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10501 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10561 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10621 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10681 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10741 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10801 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10861 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10921 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    10981 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11041 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11101 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11161 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11221 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11281 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11341 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11401 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11461 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11521 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11581 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11641 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11701 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11761 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11821 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11881 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    11941 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12001 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12061 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12121 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12181 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12241 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12301 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12361 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12421 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12481 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12541 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12601 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12661 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12721 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12781 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12841 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12901 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    12961 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13021 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13081 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13141 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13201 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13261 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13321 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13381 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13441 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13501 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13561 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13621 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13681 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13741 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13801 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13861 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13921 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    13981 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14041 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14101 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14161 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14221 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14281 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14341 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14401 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14461 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14521 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14581 ccaacctggc gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14641 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14701 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14761 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14821 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14881 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    14941 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15001 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15061 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15121 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15181 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15241 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15301 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15361 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15421 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15481 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15541 agctgcaact gttcctgtac taaccttgtt aaggttagac aaatcattct ggcttgatct
    15601 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15661 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15721 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15781 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15841 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15901 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    15961 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16021 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16081 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16141 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16201 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16261 gcgtataccg tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag
    16321 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16381 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16441 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16501 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16561 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16621 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16681 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16741 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16801 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16861 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16921 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    16981 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17041 agatgaaata caatacgaag aatatcgtaa acggaaccca aatatctttg tatggatttt
    17101 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17161 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17221 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17281 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17341 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17401 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17461 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17521 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17581 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17641 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17701 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17761 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17821 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17881 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    17941 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18001 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18061 tgaaataagt tcctaatacg atcgtaacta aggctacccc tataccaaag acttgatagg
    18121 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18181 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18241 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18301 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18361 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18421 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18481 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18541 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18601 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18661 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18721 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18781 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18841 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18901 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    18961 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19021 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19081 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19141 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19201 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19261 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19321 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19381 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19441 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19501 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19561 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19621 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19681 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19741 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19801 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19861 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19921 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    19981 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20041 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20101 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20161 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20221 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20281 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20341 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20401 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20461 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20521 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20581 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20641 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20701 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20761 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20821 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20881 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    20941 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21001 ataaatatct aatgttactt ttttaatttt tttttcgaat acgcgccatt acaccttcta
    21061 gttctaactc tttgtcttca gtgatgtacc aaccgcttgc ctgtgtccct ccggctgcct
    21121 gcggtaaata atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta
    21181 ccaagacctc tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga
    21241 taaatcgttc cttcttccca tcatacttca atattcctgt atcgataatc ggtaaagaat
    21301 aataatccag ctctttcttc accgtcaata aatgtttgaa aacttcatgg tacacttgct
    21361 ctaattggcg accttctgaa ttgttcgggt aattaaccat cactttttct ttgtctggat
    21421 catagaaggt ttggcttagt tgagtgatat aagtatgtaa tctttccatt tctgttttta
    21481 acttttctct ttgaccttct tctaccttca tatactcttt caccctgttt tctttttaga
    21541 tattttcttt cttattttac tatagttatg tgcgttaagc gatgaaaaac agcaaagatt
    21601 ggaaggatct ttgctgtttt ttcttaaaat atccagttga gtacaggatc aatctgtttc
    21661 attgtcctct tgatccgtcg taatattgat cgatgtgtct ttagcagtga tctttagctt
    21721 tttctgtcct tgttctaaca aagtatttgt tattggctgg tagtcttttt caatataaga
    21781 ttcaccagac gtttggatag tatagccact gttttccatc tcatgcaaag ataaattgcc
    21841 accgtttagg atgacttttc cttctttgct gatcaaactg tcttctagat ggaagttgct
    21901 gttttcacct gtgacgatca agttatccac attggcctca tctaatcgaa gtgagcctaa
    21961 ttcatctttc gcagttattg ttcctcctgt aaattcattg aacgataccc agccatcatg
    22021 tgtcttagta ttcacttttt tgattgatac ttcgttgata tcaattgcac tattattccc
    22081 ttcgattgtc aattcagata attcatgatc tgcagggaca acaatagtga tttgtgcact
    22141 tgtgtcttct aaactaatga ttgcacctgt tgattttttg actgcattca cagtcaacac
    22201 gccatttttc tcctttattt ctggcttttc tgtcttatca taagtcgttt ttatctcaaa
    22261 atccaatccg cgtatgaccg aaactgtttg attttctgca tggatgacga ttttgtttgc
    22321 atccttgatt ttttttgtct cagtaaccat atcgatcaac cgaggaccat tatcccaagt
    22381 cagactcttt tgagcgccta atgtataagt tgcacctgct aggaggccac cagtaagcat
    22441 cagaccaata ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac
    22501 gcccgctgaa tgcagatctc tttttgtaat aaaacgtcgt cccatccatt taacaaagtt
    22561 catcatgaca taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa taaatactgc
    22621 gcttcctgtg gcgaacagac cgacaccgat aaagaataat ccactcagaa tcgattgtcc
    22681 aaacacactg attcctgaga tgatcatgaa taggccaaca cctaagatac tgatacataa
    22741 aacatagata ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa taaccaatat
    22801 aaaccccagt gctagtggaa tgaggacagg agaagcaaat aaaccgataa tgattaacca
    22861 aatcaggcga atttgtcttt ttggattttt cactaactcg ttttctgctt gttcttcttc
    22921 tgaaaagtaa ctgattttta attttaaggc aaactgcttt ggtgtaccat attcatcaat
    22981 gatctgatct aatgtttttc cagaatctaa aaaatattcc tcataatact ggatcaaatc
    23041 aaaacgatcg tcttcaggaa tttcttgaag atgatccaca acttcattca aatattcatt
    23101 catttgtgct ctcctccaat aaccggtcta aagaatctcg aaaaacaatc cattcatcaa
    23161 taatttcttc taaatgacga cttcccattg gtgtcaacga gtaatatcga cggtttctgc
    23221 cttcaaaagg ttcatcatag gtctcaagat atccttcttt tttcaatctt ctcaaaaccg
    23281 gatatagtgt agattcactg acagagagac tttcttgtac gatttttgtt aaggcatacc
    23341 catacatatc ttccttgctt aatgctgcta gcacagtacc atcaagaaga acagttggaa
    23401 tctggatgtg cattacgtca ccttctattc tcaagtgtaa tattttattt caatctatat
    23461 tatacaacat ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa
    23521 gatttgatcc tttttagtca gcaaaaaatg gcttctcttt gatttttatt ataacagtgg
    23581 cataatccaa tttgtccaca tccagctcag tggcatgata acgatcattg caggtatcat
    23641 gtcagctact gggaacattt tcacgttgat cattctaaaa ccggtagcga ccatcaagaa
    23701 tccgccgcaa gctttaaaat ctaagatcat gtctggtgta gtcaatggat aaataaattt
    23761 tgccaataag aacaaaatat aaaaaataat gaattgggga attgctatca cagaaacgac
    23821 ataccctaaa ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat
    23881 caaaatcgta ctgtctcctg tcatcccaga atctaaactt ccatagatcc ccgtaccact
    23941 tgcacaaaat agaacaatga ccgtcactaa tgtgctgata aattcatcgt gggtcatacc
    24001 aagcttttca ctagggaaga ttttagaaat aggtacttgc atcagcgcag cccccttatt
    24061 tatcccatta cctaaatgaa caatcaaacc aatcgctgtt cctatcacta aagcaaaaat
    24121 aacagccggc atatttttca tcggcgcaat ggaatagatt cccatcccca tcgaacacac
    24181 accaaaaacc atcgtcagct gtgctttaaa atgttcactt aatttatttc ctactaatcc
    24241 gccaattagt ccgccaaaca aaacggaacc tacattgatc aaaactccag ttggcatcaa
    24301 aaaactcctc tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt aattattgct
    24361 ttcgctgcaa gaaagattgt aacagacaga gaagattaca ccaattcatt ttttgtgtta
    24421 aagtattctt tattggaata agtaggagga atatgatgga tatccataaa ttcgaagttt
    24481 ttcttgattt agccgaaaca atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc
    24541 aaggaaatat ttctaaacag attctttcgt tagaaacaga attagacgtc aaattattcg
    24601 aacgcgccca tagaaaaatc gaattgaccg aagctggaag cttattgctc ccatatgtaa
    24661 aaaatgtcgt agaacaatac catgctatgc aagaagtgct tgaaacctat acgaaagaca
    24721 acaatctttc attaaatatc ttaacgatac cgacaatgat caattataaa ggtttttcca
    24781 aaatcaccga gtttttgaaa cgccatcctg aatttacagt ccagttgaaa gaagtggaaa
    24841 gtgtcgaatt aacttacaat gacgccaatg aacatagtga cacgatccac tttgctagaa
    24901 gtttccaggc cccaccaagt tcgattgagt ggttgcctac tgaaacagac gattttgtag
    24961 cagttctacc taaaaatcat cctttagcta gtaaaaagaa attggatcta agcgaactga
    25021 aaaaggagaa ctttttgctc cttggcccca ctacgaactt gtatcaacct gtactagatc
    25081 tgtgtcgcga agctggattt gaaccaaaaa tctcttataa aggagcaaga atcgacttga
    25141 tcatcaatat gattgcaaat gacatgggga ttgctattgt tatggaaaaa acagtcaaga
    25201 atctattaaa agaaaatact gtgattctcc ctatttctcc aacaacagag agttatcttg
    25261 catttactcg taaagtcggt gagcattctt tggcttcaga tacattttgg gagtatttaa
    25321 atcaagaaga aaactaagcc ctacattttc tgtccatcta tccacccaaa aaaagagagc
    25381 gacgcatgac tttcgctctc tttcttgcat catgtccttg tttcaatgac acctctaaca
    25441 tttctcgtag ttcaataaga aagcagcgat ttttatgcca aaagaaccgt aagaacattt
    25501 ccctaatcac aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt
    25561 tttattcttt ttcctattgt ttacttaacc gtatcattgc tgtcgttcct actccttcga
    25621 tactgctata atcaatacgt ccatcatgca gttctagaat ttttttaacc agtgaaagtc
    25681 ctagcccatt cccagaaatt tgacgacttg tatctccttg ataaaatttt tcaaacattc
    25741 gatctctggt ctcttcattc attcctacac ctgaatcggt cacttccagt acaatctctg
    25801 tggctgtctc aaatagtttg atatgaatct gtccattgat ttgattgtac tttatggcgt
    25861 tatccatgat attcagccac acttggtaca aaaactcttc attgcctgtg taatttactg
    25921 cagcaagttc gatatccaat tctagctgtt ctttctccca tttaggctga aaaaaaacga
    25981 ttacttctct gatttgttca tctaatcgat actctttctt ttccagttgg atacgctggt
    26041 tttccagttt gttcaatttt aagatgttct ctgttaattg tgaaagctgc gtgatacttt
    26101 cgataatccg atataaaaat atttggcgtt cttcgtcaga gagatttggg gcttgtaaca
    26161 attgaacata tccttggatt gttgctagtg gtgttttgaa ttcatgggat acactggaca
    26221 caaagtcatt cctcagtgtc tcgatactat tgagttcttg gaccataacg ttgaaatctt
    26281 tatacagttg ctgtacttct gctactttct gctgttcatc cattcgaatc gaaaaatctc
    26341 ccgttgctac cagactcata tttgtcccta gcgcactgat tggatgcagg atacgccgcc
    26401 caacaaaaat cgatattcct gtacccacga ccatactgaa ccccgcaaag acgaataacg
    26461 ggtataacgt tggttttcca gccgattcag caggcgagat agaatgtcga gacaataaga
    26521 aaataaaacc gagaaaacac agcagtgtca ctaagatcga aatgaagaca atactagcaa
    26581 aatagatcca taattgtgaa aatagctttt ttttcatctt taaataaccg ccttatagcc
    26641 tagtccccgt actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg tccttaaacg
    26701 tttgatatgt acatcgatcg ttcgttcttc tgtattcgta tctaatcccc aaatatcatc
    26761 catcaactgt tggcgagtga agatcttgtt gggataagac agtaatttat acagaagcag
    26821 aaattctttt tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat aagcatctag
    26881 ctctaatttt gtttgtccga ttatcaagct actgctgtaa ttgatctttg aacgtcgaag
    26941 caatgcttcc acacgtagga tcatctcatt gacatcgatg ggttttgtca tataatcatc
    27001 tgctcctaat ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg taatcattag
    27061 aacaggtgtg tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc catccatttt
    27121 cggcatcatg atatctgtaa tgattaattc tatacgtgtc gcttctagtt ttttcagcgc
    27181 atcctctcca ttcaatgcgg tgatgactga aaagcccgct cgttccaaca cagaacggta
    27241 aagcaactgt aattgcggat catcttctac tactaaaata cgattcattg atctccctct
    27301 gttttttgaa aaatttctag attttttctt gtagttcatc ttcagttaac atttatttcg
    27361 tatactacga tcattgttct gatatactta ttagtatacc gaatatttca atacgggaga
    27421 agggtgatta tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg cgaaaccacc
    27481 accaaagcat tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt agcgatttta
    27541 ggtcctagtg gttctggtaa aacgactatg ctgaatgtga ttggtggact agataattat
    27601 gattccggtg atatggtaat caacggaaag tctactaaag actttaagga cagtgactgg
    27661 gatgcctacc gaaacaactc tatcggtttc atttttcaaa gttacaactt gattggtcat
    27721 ctaggcatta ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc taaagacgaa
    27781 aaacgaaaac gggcagaaga tgcattgcac cgagtcggct tgacaaatca tatgcataaa
    27841 aagccaaatc agttatctgg tggacaaatg cagcgagtgg ccatcgctcg agcactagcc
    27901 aatgacccag atatcctttt gtgcgatgaa ccaaccggag cgttagatac agaaacaagt
    27961 atccagatca tggaattgat ccaagagtta tcgaaagaaa aattagtgat catggtcaca
    28021 cataatcctg aactggccca tcaatacgca gatcgaatca ttgaattttc tgatggtaag
    28081 atcttaacag attcccatcc tcacatcgaa cgaccaaaag atgatcaatt taacttacgc
    28141 agaacaaaga tgagtttttg gacagcgtta aagctttcct tcaacaatat ccgaacaaaa
    28201 aaaggacgta ctttcttgac ctcctttgct tccagtattg ggatcatcgg aatcgcgatc
    28261 gttctgtccc tttcttctgg attccaaaaa caaatcgata acacgcaagc tgaaacgatg
    28321 gcgaaatttc cgattactat ttctaaggta acgactaacc agactcgtga cgatgccgga
    28381 ttgggtgcaa gcaaagcgga ttatccagac agcaaaacga ttactgcaaa agtcagcgaa
    28441 gaagataaag cacagcacac gaataagatt gatcaaacat acgtcgatta cgtaacagac
    28501 atcgatccta acttaagtaa taatatcgga tttacccgta ctacaggaat caatctttta
    28561 cgtgatgtaa atggcaaagt acagcctgtc agcttctcga atcaaaatcc tgatacggaa
    28621 tctctttctt tatctagtac gatgtcagca atgacaggtg tgggagtgtc aagtttccct
    28681 acacagcttg acactagcaa agaaaacttt ttaaaagaca attattcttt acttgccgga
    28741 agttatccag cctcagcaac agatgtcgtg ctgatcgtag acggcaataa taatactaat
    28801 atcaacgcat tgaagaatct tggatttgat gtgaaagaag acgaaaaact tgattttgat
    28861 gatatcgttg gtacaacatt caaattagta aacaacaata cttactatac aaaacttcca
    28921 actgggaatt ttatccctaa tacagattat gatgcgatgt atcagaatgc ttctgatgaa
    28981 ttgaagatct cggggatcct acgcgtgaaa tcttcttcta caatgaatct cttatctcca
    29041 ggaatcgctt atagcgatca attaacgact caaatcgtca acgaaaacaa agaatcagaa
    29101 atcgtcaaag ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa agttgacgaa
    29161 aatgccaaac aaacattgct tagttattta ggtggagaca gcttgccttc aagtatcatg
    29221 atttatccga acaattttga agacaaagaa aagatcttgg attatctaga tgattacaat
    29281 aaaggcaaat cagatgaaga taaaatcatt tatactgatt tagctggaac gatgactgaa
    29341 ctgactggcg gattgatgga tgctatcact tatgtcctta ttgcttttgc agggatctct
    29401 ttagtaacaa gtatgatcat gatcagtatc attacttata catctgtgat tgaacggaca
    29461 aaagaaatcg gtgtactaaa agctttaggt gcacgtaaaa aagacatcac tcgcgtattt
    29521 gacgcagaaa cgtgtatttt aggtatttct tctgggatat taggtgtgtt tattgcatgg
    29581 ctagctacgt tccctatcaa tagcatctta tacaacatga cggatctaaa aaatgttgct
    29641 caactaaatc ctgtccatgc gattattcta gtgatcgtct ctactatatt gacgatgctt
    29701 ggcggtcatc ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc tttgcgtgca
    29761 gaataaggtt gtgacaacca catttagatt tgagtcataa gagcgctgaa atagtagaaa
    29821 acagcaagaa acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt taattatttt
    29881 ttataattct ggtatacttt tctttccttt gtaccacaac tcttctggcg tatacctgtc
    29941 ttttccattt atgaatactg ttccatctgc ttcaatcgtt acttcagtcc caatccacat
    30001 attacgagaa agtgcgagaa gattttttct ttttcctctt cctttttttc caaattgcaa
    30061 ggcatcggtt atctcttctg tccaatgaga tttcacgtat tttacttctc cgttttcaaa
    30121 tgtaactttc aaaatattcg ctatattttc cacttttact gctttaggaa atgactcctc
    30181 catttttttc actttccttc ctccaacact aagtatgcgc tttcttttag tgataagtat
    30241 atcatagttt attctattat caaaaaaacg catccattcc attgtagaat actttaccga
    30301 acaagttgaa ttgttttcct ctttttcacc ttttactata gataggtaaa gacaactaag
    30361 gaggaatctc tcatgacaga tcaaccgtat gatttaagaa aagtcatcga agaactaaaa
    30421 acacttccag gtcaatatca cgaaacagat attgaaatcg atcctgaagc agaattatct
    30481 ggtgtttacc gttatatcgg tgctggcggt acagtaaaac gtccgactca agaaggacca
    30541 gctatgactt tcaacaatat caaaggtttc cccggcacac gtgtgaacat cggaacaatg
    30601 gctagtcgta aacgtgtagg tgaaattttg caccatgatt atcgaacact tggtcgtttc
    30661 ttgaaagacg ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc agacgctcca
    30721 gcacaagaag tcgttcatct agctaccgat gcagacttcg atattcgcaa cattttgcct
    30781 gcacctacaa atactcctga agatgctggt ccatacatca caatgggtgt agtcttagga
    30841 tcagatccag aaaaaacaat gagcgatgta acgatccatc gtatggttct agaagataaa
    30901 gatacgatcg gtatgtacat catgcctggc ggacgccaca ttggtcactt ccaaaaacaa
    30961 ttcgaagcct tgaataaacc aatgcctatc actatcaata ttggtttaga tccagcaatt
    31021 gccattggca cgactttcga accacctacc actccacttg gctacaatga actatggtgt
    31081 gcaggagctt tgcgtaacga acctattcaa atcgttgatg gtatagcagt cgatgaagtg
    31141 ggtattgctc gtgctgaatt catcattgaa gcagaaatca tgccaaatga aacaatgcaa
    31201 gaagatatca acacgaactc aggaaaagca atgccagaat ttcctggcta tactggggat
    31261 gctaatccag ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa agacaatcca
    31321 atcatgcaaa caacgattgg tcccagtgaa gaacacgtat ctatggccgg tattcctaca
    31381 gaagcaagta ttcttgcctt ggtcgataaa gctattccag gaaaagtagt aaatgtctac
    31441 aatcctcctg caggtggtgg taaattgatg accatcatgc aaatccgtaa agaaaacgaa
    31501 gcggatgaag gtattcagcg tcaagcagct attcttgctt tatcagcctt caaagaattg
    31561 aagacagtca ttttagtcga tgaagatgtt gatattttcg acatgaacga tgtagtttgg
    31621 actttcaaca ctcgtttcca aggagataaa gatatcatcg tgatcccagg aatgaggaac
    31681 catccattgg acccatcaga attaccagaa tataatcctg gctctattcg tttccgtggg
    31741 ataagtgcaa aaactatctt tgacggtaca gttcctttcg atatgaaaga acattttgta
    31801 cgtgcaaaat tccaagaagt agaagattgg gaaaaatatt tgaaataatg aatgatctgc
    31861 aactgttttg acatcataaa aaggagtgcg gaaaatggaa caattggcac aacggaagaa
    31921 aaaaatcgtg attggtgtaa gtggcgcttc aggcaccatt tatgccatca acctagtgaa
    31981 gaaactaaaa gaatatccga tgatagaaac acacggaatc atcagtccat gggcaaaaca
    32041 gaacttaaag ctggaatccg acttatcact aactgaattc gaaagcctgt ttgattacca
    32101 ttattcaaac aaagatttag gtgctgctgt cgccagcgga tcatttttga ccgatggaat
    32161 gatcatcgta ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt taggcgacaa
    32221 cctgattgca cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag tcattgttcc
    32281 tcgtgaaacg ccattaaatg ccattcactt ggaaaacttg actaaattag cacgactagg
    32341 tgtgcaaatc ataccaccaa ttcctgcttt ttataatcag cctcaaacga tccaagatct
    32401 tgttaatcac catacaatga aacttttgga tcaatttgaa attgtaaatg agcattcaaa
    32461 acgttggcag ggtgattaat atggaaaaag aaacgatagt tgaagtaacc aaactaggtt
    32521 tcacgatcca agctgctgtt aaagtaatcg gtaaagactt attgatcact ttaacaggcg
    32581 gagatacacc acatatcgga acagtcacta cattttctcg tgatacagag atgcaaactg
    32641 tacgtttccc tagccacgat ggtcggttcc ataaagacga tgtcttagcc gaaaaaatag
    32701 ctgtgatcat caaaactgcc gttccgggaa gttgtacgat cacttccggt atccatgttg
    32761 accatatctc caatgcacaa atcaacgcct cttttccgat gtcagaagag ttaggttaca
    32821 agattttaga ttggttggaa aaaatagatt tccatatcaa ataaccagtt tattataaag
    32881 atggtgaaaa accattatga aagaagaggt tgtgaaatca gcgttatgac ctgagtatga
    32941 gagaaaaaaa tggaaaaata gcttctcata ttttgccgtt tttgagctaa taccgaggga
    33001 cagctgattg aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt tcgagtagta
    33061 agaaaaattt tgtaaacatg ggtttccaca tttttgcata aatttgactt actaccgaga
    33121 aactggcttt tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc acaacctctt
    33181 ttatttttgc ttcactgtaa ctgctacaat atcaccgatt tcttttccga tgtttttacg
    33241 aatatctttt cgtagtccaa ggatgtagca gactgaacca tctggatttt tcacgcccat
    33301 attcacgata ctgccttcat acggcacatt gtcaaaagtt gcttggactt taactcttcc
    33361 agcaccgaat tcttttctta tatcatacgg aaaaataata tatgccccgc ccttgccgac
    33421 ttcggaagca tagatttttg attcaaaaga ataaatttcc ctcattttat ttctcctatt
    33481 ttttacatct agtatagcat agcaaaaaaa agaaggaact gtgacaaaag tcttgtcgca
    33541 gttccttttt tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag
    33601 tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca
    33661 ggctcacaac ctcttcctta aacggtgttc aaaagcttgc atctacggca ctaagcccaa
    33721 tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc
    33781 agctttttca caacctccta catggtgttc aaaatattat tcgccttttg cttctttcac
    33841 taattttgcg aatgcttgtt gttggaattc agcagctaag gctttcgtac gttgttcttg
    33901 ctctgctttg ctcatttttg gattttcagt cattgctttt accataccgt cttgaacata
    33961 agcttttgct tcactttcca tcgtactttg ctgtttcttc actaattcag ctaacttggc
    34021 tgcttggtcc gtacttaatt ctacccatgt ctctgggatt ttgaatgtat tcactcggct
    34081 gtcatattcg tgattgttat cagcaatatt gaaccagaat ttatacatgt cgtttttgta
    34141 ttcccaataa gtagttttcg tcactttttc agcatcgccg tctgtctttt ctactttatt
    34201 tgtttcgtta tccgtaccag ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag
    34261 atacactttt tcttttccat tccctaaagc tttatacaaa agcatatctg ctccttggga
    34321 atcagcactg gaaacaattt tttctgtttt tgtttccgtt actttttcca ttccaaagtg
    34381 attggacaaa ttagcgacga tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa
    34441 agataaaaag gtttgccatg tttttttagc gaacacgttc gttaaagcaa aagcaagcaa
    34501 gctgacaatc aaaactccaa taatcatgca ttatcccccg ctttcattag aacaggttct
    34561 tttttcttca ataaaaaggc aataataaag ccaagaatcc caaagatgat cgctacgata
    34621 aacgcggcat ggtaacctga cagtgtggca ttgattgcct gatctttata agctaatggc
    34681 gtattatcca atacagattt gcttgggata tttgcttttg tcacattcgt caacacactg
    34741 atcaaaatag ctgtaccaat cgagcttgcg atttgacgga atgtattatt taccgctgtc
    34801 ccatgactga tcaagttatt tggcaatgca ttcataccag aagtcgttac cggcatcatg
    34861 accatggaga tcccaaacat acggatcgca taaaggatca cgatatataa aactggagtc
    34921 gtttttgtca agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg
    34981 gctaaacgtt tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat catagccccc
    35041 atgatcaatg cacccggcaa caacatcaag ccagaatgga atgctgattc tccacgaata
    35101 ttttgaatat acaatggtac gaccatttct gcccctacca tcgccatatt tgttacacca
    35161 gctaaaacag ctgcaatcgt gaatgtagat gatttgaata cacgaagttc caagaatgga
    35221 tgttccaaat gtaattgacg ccatgagaac aaggcgatga aaatcacacc aacaactaag
    35281 aaaccaatta ctttcaagct tccccagccg tcatctccta cactagaaaa tccgtaaagt
    35341 aaactaccga atccaatcgt tgataaaata gcggataaaa tatcaatgct aggatttgac
    35401 aatggaataa cacttttcat taggaagaat gctaaaacca agacaagaac aacgattggc
    35461 agtaccatac caaacaagtc acgccaatgg tagttatcaa tgatataacc agataatgta
    35521 gggcctaaag caggagcaag tccaataacg atccctgaca tccccattgc tgatcctctt
    35581 ttttctggcg ggaaaataga tagcatgata ttttgcaata atggcatcga tactcccaca
    35641 ccgcaagctt ggatcaaacg accgattagt aatgtactaa aattaggtgc tgtaaagcaa
    35701 gtaatcgttc cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct tgaactgaat
    35761 ttattgatca gccatgctgt tattgggatc atgatcccgt tgactagcaa gaaaccagtc
    35821 gttaaccatt gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct
    35881 gtcgtcaata aggtctgatt caaaacggta caaaatgtac caattaataa gacaacaact
    35941 aaaagtgtcc gattatatgg ttttccgtaa atatctacgg gttggttgtt actcatattt
    36001 ctccttcttt ctgaattttt tctgaaacta ctagaatact ttagtcctaa attcgctatt
    36061 tgtcaaccta cttgacacat aagttaatac ggatataatt aagggaagca agaggacaaa
    36121 aggagtgaca aaaatggttg gggcttccct taaacgtttg ttggaaaatg atcaattggt
    36181 tgttggcatc agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg
    36241 ggaacaaaag ggattcattc gttcgatcga agctgatgcg aattgtccta gaaaatatcg
    36301 tttacctaca gtcataaaag tagaactgat caaaaaatat ttagatgacg gatttacttt
    36361 gtcaaaagct gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt
    36421 tttttcaaaa gcaatcaaag atatcgaatt gatcgacgaa cgctatacga ttttacaaat
    36481 cggtaacttc aaagaagaga atgctaagat gtacatcatt catgacaacg agacagacga
    36541 attaacttac aagctccttc ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga
    36601 actctaaacg ccactaatct cctaagtacc aagcttttta ataagtagat gctacataaa
    36661 tacttgaaaa aaggcctgtt tgaacataag aatatccgaa cggtgatctg cgtttctttc
    36721 atatagaaag gaattctcca tcactgttcg gatatttttt tatttttttc ggttactttc
    36781 ttctaataca tctgttagcc aatttgcata ttctgtttcc cgattgatcg catgcgctaa
    36841 aatcaggtag tgtccgtaat tttcatgttt ttctttttca gtaggaaaga tcgttttttt
    36901 tcgctctaat agatgaagtc gtttttcttc atgaaggtca cgctgctgct gaataatttc
    36961 tactaatgcc ggatcattca cgtctttgac gaaatacaat ttcagaacaa attcgtctcg
    37021 attaaccggt agttcattgg tgggtgtatg aatccactct gccaattgtt ccttcccttc
    37081 ttctgttaat tcataaactt tcttttccaa tttttcacct gtgatctcta cttgatgctg
    37141 aatgattcct tgctcttcca tttttgcaag ttcaggataa atctgactat gttttgcttg
    37201 ccaaaattct cctatttcat ttttgaacga ttgattcaat tcatatcctg tcatcttctt
    37261 cttacttaat aaccctaaca aaatatatcg aagtgtattt tttcttgcca tcagttttcc
    37321 ctccttgaca aatctttaaa tccattctat cataaatgag taatcgaaaa catgtagttt
    37381 attacatgtt ttaatttata aggagatgct taaacatgaa agaatttaaa gaattggaag
    37441 attttattgg aactcacttt atctatacgt atgacaatgg ttgggaatat gaatggtatg
    37501 ccaaaaatga ccatactgtt gattaccgta tccatggcgg aatggtcaaa ggccgttggg
    37561 taaaagacca agaagctgat atcgttaaat tgacggacgg cgtattcaaa atcacttgga
    37621 cagagccaac agggacagac gtggcattgg actttatgcc aaatgaaaag aaacttcacg
    37681 gaatgatctt tttccctaaa tgggttgaag aacatccaga aatcactgtt actttccaaa
    37741 acgaacatat cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat ccaaaattac
    37801 ttgttcctga atttgctacg atcacttata tgggtgatgc aggtatagac aatgaagatg
    37861 ttattagcga agaaccatac gatggaatgc cagatgatat ccgtagcgga aaatacttcg
    37921 atgaaaatta taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct
    37981 gacctgagta ttagagaaag aaacggaaaa atagttcctc atgttttgcc gttttttgag
    38041 ctaataccga gggtcagctg attgaacacc gtttatcaag aggttgtgaa atcagcgtta
    38101 tgctttgagt aataagaacg ctagttagaa aatagctttt catattttct aggctagtgg
    38161 gcttattacc gagaagcagc cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt
    38221 tttgacctga gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt
    38281 gagctaatac cgagggtcag ctgattgaac accgtttatc aaagattgta acaactgcat
    38341 attcacacag ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc
    38401 tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc catttcatca
    38461 caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg
    38521 aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc agtgttttat
    38581 tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg tattcccttt
    38641 tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata atttcttgat
    38701 ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat tctacttgat
    38761 tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc agccaaatcg
    38821 atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc attttttctc
    38881 tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct tttacaagtt
    38941 ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca aaagaaacag
    39001 caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat tgatccagct
    39061 tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta gtaagtgttt
    39121 ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac gaatcactaa
    39181 taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt agtcgtccga
    39241 taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac cattgccgca
    39301 tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta taaggaatcc
    39361 gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg
    39421 taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc agtaccactt
    39481 gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata aacgtgtctc
    39541 cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag
    39601 agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc aagacgcctg
    39661 ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt aattggatct
    39721 taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt gtactttcat
    39781 ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca gttaatcgtt
    39841 ggtttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc cgtttttgta
    39901 cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa aatggatata
    39961 aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac aaaagtcgac
    40021 gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt
    40081 tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa
    40141 agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat attttttgtt
    40201 gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    40261 aaactgcgtt atgctttgag taataagagc gctagtcaga aaatagcttc tcatattttc
    40321 taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc gagaggtcat
    40381 gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa
    40441 caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc ttgtaaaatt
    40501 cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg atatatgatg
    40561 aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc atttttagca
    40621 atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa actaattttg
    40681 ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat
    40741 ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag gatcgtgaat
    40801 gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct tgcaggtagc
    40861 tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg attttctatg
    40921 ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt agccgtttta
    40981 ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac tagctggggg
    41041 acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca taaaaagttc
    41101 cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt actgtcttcg
    41161 atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa ccagactgaa
    41221 aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt tctgacagca
    41281 aatatttggg caggattagc tattgtttac agtgggaata agattcttcc tccctttgtt
    41341 aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct ccattaccct
    41401 aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt tcaacaaaag
    41461 aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt tctttctact
    41521 agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag tttgatccag
    41581 tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct tgccctttca
    41641 ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg cattcttatt
    41701 ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga aaatgaaaca
    41761 tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa aacattcttc
    41821 agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca
    41881 atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat actagaaaac
    41941 gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg gaaaaacact
    42001 ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct acagcaaaat
    42061 ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt gaagcagcaa
    42121 aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta tttcattctt
    42181 tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca tagatacaag
    42241 cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct ccaaacggta
    42301 taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta tattttttat
    42361 tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg actatcgatc
    42421 ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa aataaaatct
    42481 atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac agtagctgct
    42541 tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact ttctcttaca
    42601 gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa aagtggtacg
    42661 cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc acttttttta
    42721 tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc tatctttgtt
    42781 ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgaaattg ggagacggta
    42841 aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc atatttatca
    42901 taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccgtgttc ctttaaaagc
    42961 tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt taccggacag
    43021 aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt gatttcttgt
    43081 aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg caattttttc
    43141 tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat tggttttctc
    43201 cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac ctctccagca
    43261 aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag ctattttttg
    43321 tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc actaaacagt
    43381 attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga ggagctattt
    43441 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tccttacatt
    43501 ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat cactacttct
    43561 tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc gttttggatc
    43621 acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc ttctttgaca
    43681 gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg gatcaaaact
    43741 tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat acttgtcact
    43801 tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa gccgtcaccc
    43861 atcatttttt gtgagaatac cggatctttt acttcagata aaggaatcac ttctccagaa
    43921 gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc ttgtccattt
    43981 cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag atcttcgata
    44041 tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt gtctttgacg
    44101 atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt ttctcgatca
    44161 tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt ttcttttcct
    44221 cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc gctgttagat
    44281 acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt agcaaagttg
    44341 aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc gatcacacac
    44401 aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg tgtccgagtc
    44461 agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc catcgcaaat
    44521 gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa cataaacatg
    44581 aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga aaaatacatt
    44641 gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa agccattccc
    44701 atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg cgtccagtta
    44761 tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac taaatcagtg
    44821 gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg tgcgcctgat
    44881 aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag catatgatgc
    44941 aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc tagaatcggt
    45001 gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc ctgtacgttt
    45061 ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac aacaaacgga
    45121 acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata attatagtac
    45181 ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac tcccatattc
    45241 aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac
    45301 agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc aaagatagcc
    45361 ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc gcctgctctt
    45421 tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt accaataatt
    45481 gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca atcaggattg
    45541 atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat aactgctacc
    45601 acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat ctttttcatt
    45661 ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc gttttctttt
    45721 ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc tataatacgt
    45781 gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt tctatgaaat
    45841 attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg tataaaacag
    45901 ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa
    45961 atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt
    46021 ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat
    46081 caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa
    46141 aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat
    46201 atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg
    46261 gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat
    46321 aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc
    46381 tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat
    46441 ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt
    46501 ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt
    46561 gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct
    46621 gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt
    46681 gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg
    46741 cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca
    46801 aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa
    46861 ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac
    46921 tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc
    46981 ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa
    47041 cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac
    47101 aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc
    47161 gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc
    47221 tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga
    47281 ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga
    47341 gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat
    47401 aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat
    47461 ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa
    47521 gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat
    47581 gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct
    47641 ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat
    47701 gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac
    47761 tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg
    47821 attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct
    47881 cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat cacttccatg
    47941 actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag
    48001 ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat
    48061 cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa
    48121 ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa
    48181 ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt
    48241 cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg
    48301 cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact
    48361 ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga
    48421 attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga
    48481 tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc
    48541 tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta
    48601 ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt
    48661 cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc
    48721 gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc
    48781 tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta
    48841 cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta ccagttccag
    48901 agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt
    48961 acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt
    49021 tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg
    49081 tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa
    49141 actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag
    49201 tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt
    49261 ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag
    49321 agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct
    49381 acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt
    49441 tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag
    49501 aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc
    49561 cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac
    49621 aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg
    49681 tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt
    49741 caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat
    49801 ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct
    49861 ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga
    49921 aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt
    49981 ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca
    50041 tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc
    50101 ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg
    50161 aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc
    50221 tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt
    50281 attcagatag tcaagatact gtcattgatt atttatacca aaaccacatc cctttcactt
    50341 tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc
    50401 ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg
    50461 tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa
    50521 tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc
    50581 tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata
    50641 tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt ccagagacac
    50701 tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa
    50761 gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga
    50821 tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag
    50881 agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca
    50941 tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc
    51001 tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    51061 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa
    51121 aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
    51181 tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt
    51241 aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt
    51301 catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc
    51361 acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga
    51421 acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt
    51481 gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc
    51541 ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc
    51601 caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag
    51661 cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc
    51721 tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga
    51781 tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac
    51841 tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc
    51901 attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa
    51961 cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt
    52021 aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc
    52081 taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg
    52141 accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt
    52201 cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag
    52261 tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa
    52321 gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat
    52381 cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat
    52441 gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag
    52501 gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat
    52561 cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg
    52621 acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt atttttcttc
    52681 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52741 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52801 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52861 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52921 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    52981 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53041 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53101 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53161 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53221 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53281 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53341 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53401 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53461 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53521 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53581 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53641 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53701 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53761 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53821 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53881 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    53941 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54001 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54061 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54121 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54181 gggattcttt gagtttattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54241 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54301 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54361 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54421 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54481 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54541 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54601 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54661 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54721 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54781 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54841 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54901 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    54961 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55021 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55081 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55141 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55201 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55261 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55321 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55381 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55441 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55501 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55561 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55621 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55681 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55741 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55801 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55861 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55921 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    55981 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56041 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56101 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56161 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56221 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56281 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56341 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56401 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56461 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56521 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56581 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56641 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56701 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56761 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56821 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56881 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    56941 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57001 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57061 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57121 cttctgcttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57181 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57241 cgatagattg caggatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57301 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57361 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57421 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57481 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57541 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57601 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57661 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57721 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57781 tttatgaaag caggaacctg ctagcatatg taattaatta tttcttcagg ataggatagg
    57841 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57901 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    57961 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58021 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58081 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58141 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58201 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58261 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58321 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58381 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58441 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58501 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58561 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58621 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58681 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58741 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58801 tgattttgtt ttagttaatt tgaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58861 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58921 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    58981 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59041 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59101 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59161 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59221 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59281 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59341 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59401 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59461 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59521 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59581 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59641 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59701 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59761 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59821 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59881 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    59941 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60001 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60061 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60121 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60181 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60241 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60301 gttcatttgt agagcctccc gtaatt
//
LOCUS       DACCXM010000003        60165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCXM010000003 DACCXM010000000
VERSION     DACCXM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.003"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..478)
                     /locus_tag="IVY61_000119"
     CDS             complement(<1..478)
                     /locus_tag="IVY61_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAP7969263.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIG"
     gene            1097..3931
                     /locus_tag="IVY61_000120"
     CDS             1097..3931
                     /locus_tag="IVY61_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7969264.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            4122..4613
                     /locus_tag="IVY61_000121"
     CDS             4122..4613
                     /locus_tag="IVY61_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP7969265.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            4716..5705
                     /gene="manX"
                     /locus_tag="IVY61_000122"
     CDS             4716..5705
                     /gene="manX"
                     /locus_tag="IVY61_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP7969266.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            5738..6541
                     /locus_tag="IVY61_000123"
     CDS             5738..6541
                     /locus_tag="IVY61_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP7969267.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            6562..7476
                     /locus_tag="IVY61_000124"
     CDS             6562..7476
                     /locus_tag="IVY61_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP7969268.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            7559..7933
                     /locus_tag="IVY61_000125"
     CDS             7559..7933
                     /locus_tag="IVY61_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP7969269.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            8072..8317
                     /locus_tag="IVY61_000126"
     CDS             8072..8317
                     /locus_tag="IVY61_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7969270.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            8489..9553
                     /locus_tag="IVY61_000127"
     CDS             8489..9553
                     /locus_tag="IVY61_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7969271.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9799..10224
                     /locus_tag="IVY61_000128"
     CDS             9799..10224
                     /locus_tag="IVY61_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7969272.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            10217..10666
                     /gene="nusB"
                     /locus_tag="IVY61_000129"
     CDS             10217..10666
                     /gene="nusB"
                     /locus_tag="IVY61_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP7969273.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10763..11617
                     /locus_tag="IVY61_000130"
     CDS             10763..11617
                     /locus_tag="IVY61_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP7969274.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            11618..12955
                     /locus_tag="IVY61_000131"
     CDS             11618..12955
                     /locus_tag="IVY61_000131"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP7969275.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12960..13184
                     /locus_tag="IVY61_000132"
     CDS             12960..13184
                     /locus_tag="IVY61_000132"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP7969276.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            13186..14076
                     /locus_tag="IVY61_000133"
     CDS             13186..14076
                     /locus_tag="IVY61_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7969277.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            14085..14903
                     /locus_tag="IVY61_000134"
     CDS             14085..14903
                     /locus_tag="IVY61_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP7969278.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            15010..15459
                     /locus_tag="IVY61_000135"
     CDS             15010..15459
                     /locus_tag="IVY61_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7969279.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            15477..17159
                     /gene="recN"
                     /locus_tag="IVY61_000136"
     CDS             15477..17159
                     /gene="recN"
                     /locus_tag="IVY61_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP7969280.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(17214..17330)
                     /locus_tag="IVY61_000137"
     CDS             complement(17214..17330)
                     /locus_tag="IVY61_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP7969281.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(17403..18359)
                     /locus_tag="IVY61_000138"
     CDS             complement(17403..18359)
                     /locus_tag="IVY61_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7969282.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            18530..18898
                     /locus_tag="IVY61_000139"
     CDS             18530..18898
                     /locus_tag="IVY61_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP7969283.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            19068..19499
                     /gene="mraZ"
                     /locus_tag="IVY61_000140"
     CDS             19068..19499
                     /gene="mraZ"
                     /locus_tag="IVY61_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP7969284.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            19520..20479
                     /gene="rsmH"
                     /locus_tag="IVY61_000141"
     CDS             19520..20479
                     /gene="rsmH"
                     /locus_tag="IVY61_000141"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP7969285.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            20484..20885
                     /gene="ftsL"
                     /locus_tag="IVY61_000142"
     CDS             20484..20885
                     /gene="ftsL"
                     /locus_tag="IVY61_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP7969286.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20886..23078
                     /locus_tag="IVY61_000143"
     CDS             20886..23078
                     /locus_tag="IVY61_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7969287.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            23104..24066
                     /locus_tag="IVY61_000144"
     CDS             23104..24066
                     /locus_tag="IVY61_000144"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP7969288.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            24080..25441
                     /locus_tag="IVY61_000145"
     CDS             24080..25441
                     /locus_tag="IVY61_000145"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP7969289.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            25467..26555
                     /gene="murG"
                     /locus_tag="IVY61_000146"
     CDS             25467..26555
                     /gene="murG"
                     /locus_tag="IVY61_000146"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7969290.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            26570..27790
                     /locus_tag="IVY61_000147"
     CDS             26570..27790
                     /locus_tag="IVY61_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP7969291.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27946..29271
                     /gene="ftsA"
                     /locus_tag="IVY61_000148"
     CDS             27946..29271
                     /gene="ftsA"
                     /locus_tag="IVY61_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP7969292.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            29294..30535
                     /gene="ftsZ"
                     /locus_tag="IVY61_000149"
     CDS             29294..30535
                     /gene="ftsZ"
                     /locus_tag="IVY61_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP7969293.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            30552..31229
                     /locus_tag="IVY61_000150"
     CDS             30552..31229
                     /locus_tag="IVY61_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP7969294.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            31243..31842
                     /locus_tag="IVY61_000151"
     CDS             31243..31842
                     /locus_tag="IVY61_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP7969295.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31857..32144
                     /locus_tag="IVY61_000152"
     CDS             31857..32144
                     /locus_tag="IVY61_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP7969296.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            32193..32975
                     /locus_tag="IVY61_000153"
     CDS             32193..32975
                     /locus_tag="IVY61_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP7969297.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32996..33703
                     /locus_tag="IVY61_000154"
     CDS             32996..33703
                     /locus_tag="IVY61_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP7969298.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33965..36763
                     /gene="ileS"
                     /locus_tag="IVY61_000155"
     CDS             33965..36763
                     /gene="ileS"
                     /locus_tag="IVY61_000155"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP7969299.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36812..38338)
                     /locus_tag="IVY61_000156"
     CDS             complement(36812..38338)
                     /locus_tag="IVY61_000156"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP7969300.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(38353..39000)
                     /locus_tag="IVY61_000157"
     CDS             complement(38353..39000)
                     /locus_tag="IVY61_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP7969301.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(39184..39513)
                     /locus_tag="IVY61_000158"
     CDS             complement(39184..39513)
                     /locus_tag="IVY61_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP7969302.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            39690..40418
                     /gene="sfsA"
                     /locus_tag="IVY61_000159"
     CDS             39690..40418
                     /gene="sfsA"
                     /locus_tag="IVY61_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP7969303.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            40434..41447
                     /locus_tag="IVY61_000160"
     CDS             40434..41447
                     /locus_tag="IVY61_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7969304.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            41447..42724
                     /locus_tag="IVY61_000161"
     CDS             41447..42724
                     /locus_tag="IVY61_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7969305.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42787..45489
                     /locus_tag="IVY61_000162"
     CDS             42787..45489
                     /locus_tag="IVY61_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7969306.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            45641..45958
                     /locus_tag="IVY61_000163"
     CDS             45641..45958
                     /locus_tag="IVY61_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7969307.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45988..46308
                     /locus_tag="IVY61_000164"
     CDS             45988..46308
                     /locus_tag="IVY61_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7969308.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            46416..47876
                     /locus_tag="IVY61_000165"
     CDS             46416..47876
                     /locus_tag="IVY61_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7969309.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47944..48165
                     /locus_tag="IVY61_000166"
     CDS             47944..48165
                     /locus_tag="IVY61_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969310.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            48196..48378
                     /locus_tag="IVY61_000167"
     CDS             48196..48378
                     /locus_tag="IVY61_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP7969311.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            48378..48791
                     /locus_tag="IVY61_000168"
     CDS             48378..48791
                     /locus_tag="IVY61_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP7969312.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48914..50095
                     /locus_tag="IVY61_000169"
     CDS             48914..50095
                     /locus_tag="IVY61_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7969313.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            50484..50557
                     /locus_tag="IVY61_000170"
     tRNA            50484..50557
                     /locus_tag="IVY61_000170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50518..50520,aa:Arg,seq:cct)
     gene            complement(50626..51765)
                     /locus_tag="IVY61_000171"
     CDS             complement(50626..51765)
                     /locus_tag="IVY61_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7969314.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(52064..52699)
                     /locus_tag="IVY61_000172"
     CDS             complement(52064..52699)
                     /locus_tag="IVY61_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969315.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(52812..53447)
                     /locus_tag="IVY61_000173"
     CDS             complement(52812..53447)
                     /locus_tag="IVY61_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP7969316.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(53481..53942)
                     /locus_tag="IVY61_000174"
     CDS             complement(53481..53942)
                     /locus_tag="IVY61_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP7969317.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(54072..54503)
                     /locus_tag="IVY61_000175"
     CDS             complement(54072..54503)
                     /locus_tag="IVY61_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7969318.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(54521..54841)
                     /locus_tag="IVY61_000176"
     CDS             complement(54521..54841)
                     /locus_tag="IVY61_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7969319.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            55140..55916
                     /locus_tag="IVY61_000177"
     CDS             55140..55916
                     /locus_tag="IVY61_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7969320.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            55931..56134
                     /locus_tag="IVY61_000178"
     CDS             55931..56134
                     /locus_tag="IVY61_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7969321.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            56150..56488
                     /locus_tag="IVY61_000179"
     CDS             56150..56488
                     /locus_tag="IVY61_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP7969322.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            56475..56654
                     /locus_tag="IVY61_000180"
     CDS             56475..56654
                     /locus_tag="IVY61_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969323.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(56697..57167)
                     /locus_tag="IVY61_000181"
     CDS             complement(56697..57167)
                     /locus_tag="IVY61_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969324.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            57254..57952
                     /locus_tag="IVY61_000182"
     CDS             57254..57952
                     /locus_tag="IVY61_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7969325.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            58130..58471
                     /locus_tag="IVY61_000183"
     CDS             58130..58471
                     /locus_tag="IVY61_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969326.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            58464..59135
                     /locus_tag="IVY61_000184"
     CDS             58464..59135
                     /locus_tag="IVY61_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7969327.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            59141..59833
                     /locus_tag="IVY61_000185"
     CDS             59141..59833
                     /locus_tag="IVY61_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969328.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            59830..>60165
                     /locus_tag="IVY61_000186"
     CDS             59830..>60165
                     /locus_tag="IVY61_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7969329.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKD"
BASE COUNT        20061 a        10074 c        12538 g        17492 t
ORIGIN      
        1 tgcctatcat tttttgttga gctacaatta aacgtttatt agttaatgcg tatgcataat
       61 tattgtcatg cttagttgat gagatataat tatgcaatcc tacaaagcag aagattacgt
      121 attcgtcact gttaagttga tctgatatta agttaaaatg atgagttgtc cattttttag
      181 tcattccttt accgtagccg tatttaagac aaaattctac catctcttca gtggttctca
      241 ttctatcatt acctatatca tgattgtttt gtgcagtatg atcagatatg tttaattttc
      301 ttttttctga tggatcctta tctaaatctt ctaaattcaa agatcttatt attttataag
      361 gagtagtcat accgtaacca gcttttttca tacaattgcc gcaaacaatt tgtttatctt
      421 tgattttata tgaagttaag ccaattttac ctccgcactt tgcacattgt ttactcacat
      481 tagttcctcc tagatatcag ctttctacta ctattaacat tctatcaata gtgaccatac
      541 tttgaccata ctttttagaa agaatagaaa attccaagat atttaataat agaagaaaag
      601 cactaacatc atgggttcag aagaattgag agcttatcag gaaacagtat aattgaagtg
      661 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
      721 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
      781 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
      841 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
      901 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
      961 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     1021 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     1081 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     1141 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     1201 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     1261 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     1321 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     1381 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     1441 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     1501 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     1561 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     1621 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     1681 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     1741 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     1801 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     1861 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     1921 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     1981 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     2041 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     2101 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     2161 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     2221 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     2281 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     2341 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     2401 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     2461 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     2521 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     2581 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     2641 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     2701 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     2761 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     2821 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     2881 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     2941 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     3001 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     3061 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     3121 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     3181 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     3241 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     3301 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     3361 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     3421 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     3481 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     3541 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     3601 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     3661 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     3721 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     3781 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     3841 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     3901 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     3961 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     4021 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     4081 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     4141 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     4201 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     4261 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     4321 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     4381 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     4441 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     4501 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     4561 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     4621 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     4681 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
     4741 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
     4801 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
     4861 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
     4921 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
     4981 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
     5041 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
     5101 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
     5161 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
     5221 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
     5281 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
     5341 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
     5401 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
     5461 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
     5521 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
     5581 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
     5641 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
     5701 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
     5761 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
     5821 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
     5881 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
     5941 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
     6001 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
     6061 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
     6121 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
     6181 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
     6241 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
     6301 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
     6361 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
     6421 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
     6481 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
     6541 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
     6601 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
     6661 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
     6721 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
     6781 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
     6841 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
     6901 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
     6961 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
     7021 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
     7081 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
     7141 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
     7201 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
     7261 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
     7321 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
     7381 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
     7441 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
     7501 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
     7561 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
     7621 gacagattac gggcaaatca tgataggtaa taatggtttt gagttttacc atgcgcgaga
     7681 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
     7741 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
     7801 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
     7861 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
     7921 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
     7981 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
     8041 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
     8101 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
     8161 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
     8221 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
     8281 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
     8341 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
     8401 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
     8461 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
     8521 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
     8581 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
     8641 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
     8701 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
     8761 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
     8821 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
     8881 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
     8941 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
     9001 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
     9061 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
     9121 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
     9181 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
     9241 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
     9301 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
     9361 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
     9421 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
     9481 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
     9541 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
     9601 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
     9661 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
     9721 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
     9781 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
     9841 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
     9901 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
     9961 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    10021 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    10081 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    10141 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    10201 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    10261 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    10321 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    10381 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    10441 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    10501 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    10561 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    10621 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    10681 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    10741 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    10801 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    10861 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    10921 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    10981 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    11041 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    11101 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    11161 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    11221 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    11281 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    11341 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    11401 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    11461 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    11521 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    11581 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    11641 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    11701 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    11761 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    11821 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    11881 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    11941 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    12001 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    12061 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    12121 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    12181 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    12241 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    12301 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    12361 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    12421 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    12481 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    12541 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    12601 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    12661 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    12721 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    12781 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    12841 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    12901 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    12961 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    13021 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    13081 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    13141 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    13201 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    13261 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    13321 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    13381 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    13441 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    13501 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    13561 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    13621 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    13681 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    13741 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    13801 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    13861 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    13921 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    13981 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    14041 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    14101 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    14161 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    14221 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    14281 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    14341 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    14401 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    14461 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    14521 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    14581 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    14641 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    14701 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    14761 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    14821 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    14881 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    14941 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    15001 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    15061 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    15121 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    15181 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    15241 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    15301 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    15361 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    15421 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    15481 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    15541 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    15601 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    15661 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    15721 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    15781 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    15841 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    15901 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    15961 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    16021 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    16081 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    16141 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    16201 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    16261 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    16321 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    16381 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    16441 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    16501 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    16561 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    16621 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    16681 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    16741 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    16801 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    16861 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    16921 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    16981 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    17041 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    17101 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    17161 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    17221 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    17281 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    17341 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    17401 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    17461 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    17521 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    17581 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    17641 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    17701 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    17761 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    17821 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    17881 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    17941 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    18001 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    18061 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    18121 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    18181 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    18241 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    18301 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    18361 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    18421 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    18481 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    18541 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    18601 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    18661 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    18721 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    18781 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    18841 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    18901 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    18961 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    19021 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    19081 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    19141 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    19201 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    19261 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    19321 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    19381 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    19441 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    19501 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    19561 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    19621 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    19681 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    19741 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    19801 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    19861 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    19921 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    19981 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    20041 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    20101 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    20161 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    20221 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    20281 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    20341 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    20401 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    20461 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    20521 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    20581 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    20641 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    20701 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    20761 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    20821 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    20881 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    20941 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    21001 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    21061 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    21121 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    21181 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    21241 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    21301 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    21361 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    21421 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    21481 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    21541 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    21601 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    21661 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    21721 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    21781 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    21841 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    21901 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    21961 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    22021 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    22081 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    22141 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    22201 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    22261 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    22321 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    22381 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    22441 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    22501 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    22561 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    22621 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    22681 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    22741 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    22801 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    22861 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    22921 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    22981 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    23041 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    23101 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    23161 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    23221 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    23281 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    23341 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    23401 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    23461 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    23521 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    23581 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    23641 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    23701 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    23761 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    23821 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    23881 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    23941 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    24001 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    24061 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    24121 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    24181 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    24241 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    24301 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    24361 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    24421 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    24481 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    24541 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    24601 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    24661 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    24721 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    24781 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    24841 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    24901 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    24961 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    25021 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    25081 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    25141 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    25201 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    25261 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    25321 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    25381 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    25441 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    25501 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    25561 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    25621 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    25681 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    25741 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    25801 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    25861 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    25921 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    25981 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    26041 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    26101 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    26161 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    26221 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    26281 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    26341 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    26401 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    26461 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    26521 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    26581 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    26641 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    26701 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    26761 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    26821 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    26881 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    26941 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    27001 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    27061 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    27121 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    27181 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    27241 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    27301 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    27361 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    27421 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    27481 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    27541 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    27601 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    27661 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    27721 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    27781 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    27841 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    27901 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    27961 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    28021 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    28081 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    28141 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    28201 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    28261 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    28321 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    28381 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    28441 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    28501 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    28561 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    28621 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    28681 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    28741 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    28801 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    28861 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    28921 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    28981 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    29041 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    29101 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    29161 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    29221 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    29281 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    29341 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    29401 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    29461 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    29521 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    29581 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    29641 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    29701 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    29761 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    29821 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    29881 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    29941 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    30001 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    30061 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    30121 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    30181 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    30241 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    30301 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    30361 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    30421 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    30481 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    30541 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    30601 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    30661 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    30721 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    30781 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    30841 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    30901 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    30961 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    31021 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    31081 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    31141 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    31201 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    31261 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    31321 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    31381 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    31441 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    31501 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    31561 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    31621 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    31681 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    31741 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    31801 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    31861 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    31921 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    31981 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    32041 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    32101 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    32161 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    32221 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    32281 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    32341 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    32401 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    32461 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    32521 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    32581 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    32641 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    32701 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    32761 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    32821 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    32881 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    32941 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    33001 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    33061 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    33121 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    33181 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    33241 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    33301 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    33361 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    33421 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    33481 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    33541 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    33601 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    33661 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    33721 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    33781 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    33841 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    33901 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    33961 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    34021 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    34081 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    34141 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    34201 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    34261 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    34321 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    34381 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    34441 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    34501 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    34561 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    34621 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    34681 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    34741 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    34801 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    34861 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    34921 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    34981 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    35041 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    35101 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    35161 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    35221 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    35281 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    35341 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    35401 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    35461 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    35521 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    35581 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    35641 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    35701 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    35761 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    35821 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    35881 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    35941 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    36001 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    36061 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    36121 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    36181 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    36241 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    36301 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    36361 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    36421 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    36481 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    36541 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    36601 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    36661 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    36721 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    36781 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    36841 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    36901 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    36961 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    37021 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    37081 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    37141 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    37201 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    37261 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    37321 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    37381 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    37441 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    37501 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    37561 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    37621 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    37681 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    37741 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    37801 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    37861 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    37921 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    37981 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    38041 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    38101 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    38161 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    38221 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    38281 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    38341 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    38401 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    38461 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    38521 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    38581 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    38641 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    38701 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    38761 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    38821 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    38881 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    38941 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    39001 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    39061 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    39121 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    39181 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    39241 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    39301 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    39361 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    39421 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    39481 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    39541 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    39601 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    39661 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    39721 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    39781 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    39841 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    39901 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    39961 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    40021 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    40081 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    40141 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    40201 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    40261 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    40321 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    40381 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    40441 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    40501 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    40561 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    40621 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    40681 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    40741 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    40801 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    40861 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    40921 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    40981 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    41041 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    41101 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    41161 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    41221 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    41281 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    41341 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    41401 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    41461 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    41521 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    41581 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    41641 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    41701 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    41761 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    41821 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    41881 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    41941 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    42001 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    42061 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    42121 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    42181 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    42241 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    42301 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    42361 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    42421 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    42481 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    42541 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    42601 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    42661 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    42721 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    42781 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    42841 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    42901 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    42961 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    43021 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    43081 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    43141 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    43201 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    43261 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    43321 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    43381 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    43441 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    43501 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    43561 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    43621 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    43681 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    43741 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    43801 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    43861 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    43921 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    43981 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    44041 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    44101 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    44161 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    44221 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    44281 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    44341 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    44401 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    44461 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    44521 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    44581 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    44641 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    44701 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    44761 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    44821 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    44881 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    44941 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    45001 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    45061 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    45121 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    45181 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    45241 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    45301 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    45361 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    45421 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    45481 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    45541 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    45601 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    45661 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    45721 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    45781 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    45841 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    45901 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    45961 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    46021 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    46081 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    46141 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    46201 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    46261 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    46321 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    46381 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    46441 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    46501 gtgatgcttt tatcggcata ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    46561 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    46621 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    46681 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    46741 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    46801 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    46861 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    46921 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    46981 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    47041 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    47101 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    47161 aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag
    47221 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    47281 atggatcaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    47341 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    47401 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    47461 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    47521 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    47581 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    47641 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    47701 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    47761 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    47821 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    47881 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    47941 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    48001 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    48061 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    48121 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    48181 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    48241 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    48301 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    48361 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    48421 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    48481 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    48541 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    48601 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    48661 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    48721 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    48781 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    48841 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    48901 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    48961 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    49021 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    49081 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    49141 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    49201 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    49261 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    49321 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    49381 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    49441 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    49501 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    49561 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    49621 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    49681 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    49741 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    49801 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    49861 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    49921 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    49981 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    50041 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    50101 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    50161 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    50221 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    50281 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    50341 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    50401 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    50461 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    50521 aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt
    50581 gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt
    50641 atataaacta tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac
    50701 aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac
    50761 gctaagtata tttactcctt gataaagtaa aatagatgca tgtgtatgcc ttagtccatg
    50821 caaagtaaat tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat atttgctgac
    50881 ggcgtttgat gaaactaatc cattttttaa gttgaaaaaa acaaaattat gagggttttt
    50941 gatttcaaaa ttctcgaata attctttttg attgatttta aactttttta agagatcaat
    51001 cgtatgttta tcaatggaaa ttttcctatt tgaagtttcg ttttttgttt ttccccattc
    51061 atttaattta taattccatg ttttattaat agtgatgatt tgatcttcga aatcgatatc
    51121 ttcccatgtt aatcctagaa gttcagcaaa tcgcattcca gtggcaccag caactaacac
    51181 gagcatagga gaagagtact gcgcagaaag gttttcttct acgagtttca ttaaagattt
    51241 aaattgatca taatctaaat atttatcttc ttttggcttc aatgaatctt ttcctttgat
    51301 tactgctttt ctagttggat caaacggaat taatccttct tctacggcat ctttcaacga
    51361 cgctctaata tggttattaa acttaataac ggtagatttt acatggtctt tagcatactt
    51421 atttaaaaag cgttgatatc ccactctatc taaatcagaa atcaataccg ctggcatata
    51481 tttctttatg ttcattaacg tatcttcgta ttttctataa gtgataggag atactgttcc
    51541 ttctttataa agttgcatcc aatcctcaaa gtaatctgat aataatagat ttttgcgatc
    51601 ccctttaagg cctttgttca agttatattc taattcgttg gcggcatctt ttgcttctcc
    51661 ttttgtctta aatcctgatt ttcttatctt cgtgtatttt ccgtcatctt ttttgtatga
    51721 aatttcgtat tgccaactat ttccacgttt gactaatctt gccataattg aatcagctct
    51781 ctttctctga tacaatagga actataaaga agcccattgt ttgggtttta ttattttttg
    51841 cacatccttt ttgcttgccg gcgcaaggat gtgctatttg tcgttttaat ggtataattg
    51901 gatgtgtatt agatcatcca atcagaggtg tttattatga ttaaagtgat ttttaaaaga
    51961 ataactgaac ttccagagtt tagattattt gttgattatt ggtatgtctg gttttttatt
    52021 gttgcattgc tgataggtct agatgtcata ttagaaagaa aacttaagta agagttgagt
    52081 taataaattg aattcctaaa tgagatattt tgtagcccat attttgtttg ccgacatcac
    52141 ctaatactat ggagctaatc gttttttcta acagaccaaa atcagataat ctatttaagc
    52201 taccatctat tagctcttgt gaatagccag acatgctgat atttccagtg aaattttcgt
    52261 ttccacgact cattaagcat gctaacaatt gaatctcaat cgatgttaat tgattcagta
    52321 caagaagata atactcctcg ttatcccatg aagcgttgtt aattcgaagt aaacaattct
    52381 tataaagatt tttatacata tctattttat tttgtgcctt tgatttttct acctcgtcat
    52441 ttatcccttc gaatattcca atgagttggt ccttgttctc taagttgaaa acatcttctg
    52501 gaatttgatc gcgaatgttt tctaatgatt cgtttaaatt tttgtagaat ttctcgatgc
    52561 gtttgaaacg cttctcgttt ttagacccaa aataaagtga ctgcacaacg ccaccaatat
    52621 aaggaattgc tccaataccg ccttcaacta ttaattcaag atattctttt ttggttagtt
    52681 tttcattgct attatccata gttacctcct attttgtcat ttcctttaca agcattgtga
    52741 ctcctgatgg cactcttttg tataggtttt attattgtgc acaatctaac tcttagtggg
    52801 gcgtggttca tttatttttt acttacttta ctattaatca tatcccattg agcataggat
    52861 ttttctatgt tcgatcctct tgaactaatt tctcctgtaa aagttacttt atcgcccttt
    52921 gtaaaggtat cagcattgtt taagtccttc gcaaaaatta catatgaaac tttaggtgtt
    52981 gcagaaatat cattccaact tttatcatcg tactctttgt ctgctataat ggctattcta
    53041 gtagtcattg attcgataac agttccttca aaagtatatg ttttaccact aatatattta
    53101 gtaaaagctg tattttgctc agtagctgga gtaatagaat aataactatc taagaaagca
    53161 ccccagtcat gattatttgt ctctaaaaat tcttcaaatt gagttaggct tttattgctg
    53221 ctgttagatg tagtagtttg agtatcagat gatgagcttt cctgtttgtt attgtcattt
    53281 gcctcgtttg atgaggaaag cgagaaacct acggtagtaa aaataatccc gataataagg
    53341 agaatcatag gtgttttctt atttgttttt ttgataaatc ttacaattaa catgattaaa
    53401 cctataataa ataaaacaat accagtaaaa ccaaatatac cgtccattaa tagatcctct
    53461 ttctcttttg atattgaata ttataaacca agcaattctt ttttcttaaa atcgaattct
    53521 tcttgattaa taatcccttc atctaacaac tctttatact ttttaatttc atctgcactt
    53581 gaaatttgtg tttctttgga attattatct aatatagatt gttgcttctc tatatatttt
    53641 tttaaatttt ccatttcatt ccaatatttt tttgtaaaca taacggtgtt ttcatctttc
    53701 gtggccgcta atatacctcc gcgactttcg tttccgccta gcaaggtaaa ttgtatataa
    53761 ccattactca tacctggttt ttttaattgt acggctgata tatttttaaa ggggatagtt
    53821 ttttctcctt taataccttg attaactaaa tttataaatc cttttctaat aattgaaata
    53881 gaatcatcag ttactttaat caaggttttt cctggagatt ttattaaaat ctctttttcc
    53941 attttttctt cctcatttct gtgatatgat tttttttgta gaagctcata tatgaggaaa
    54001 gagcccgtgt tgcagcacgg gcttttttaa tgttttgctg aaatcggttt ttttaataat
    54061 tcttgatgta ttcatacata ttgccttgcg taagaatatt ttttcttaaa atagcattgg
    54121 cagacagcat aacaagtgta tctgagctta ttctgaatag aataatactc cataaatttt
    54181 tcgagattaa actgagattc atcagtcagt tcgttctcaa tatagatatt taataaaatt
    54241 agaatagcta ttttatctgc ttctgtttca aattttgagt gaaaagttgt agaagtatcg
    54301 tacaaaactg aaaattcaaa atgagaggcg ctgaaatgtg caagttcatg agataaatga
    54361 aaggcttctg cagtttcacc gtatagattt tcattcaaaa aaatgattct gggttttgga
    54421 tagtagaaac caggctcttt catctccata taaactactt tcaaattgta ttcgctcaac
    54481 atttctttca atttcaaata catacaaacc atcactccaa ctattcattt tcctctaaag
    54541 ctttagcaat tgcaatcgct ttacgcattg tctccttaga tatttctttt ccatcaaaag
    54601 aaaaaacagt atcgtcttct gataaatcca catgtttagg ggtttctctt tcttctctac
    54661 ctagaaggta gtctacagag acatcgaaat agtcagcaat ccgacttaat tcatctgaat
    54721 taggagtatt atttttccac tttgctagat aaccgttaga atatccaaaa ttaatttcta
    54781 actgccgtat agatatcttt ttcctcttcg ccaagtcttt tattatttca taggtattca
    54841 tcgataaaac aacctttcta aatgtttaca aaaaaagttt agaaaaatac gcagaaataa
    54901 tttgactaat tctgagtaat gagctatact atatcttgta aacagattta acaactaaaa
    54961 agacaacaaa aaacactatt gatatgtaaa tgcagaccgc caagaaagct ttaaaatcaa
    55021 tgtttttatg tcttatttaa ttatgctctt agtatagagt attactcaga tactgtcaat
    55081 tgaatttaga aaaaagttgt taaatttgtt tacgaatata aaagaaagga atgagaaata
    55141 tgaacacacc gcaaattttt aatttcgaac aaaacgaagt tcggacaatt ttagtaaatg
    55201 atgaaccata ttttgtaggc aaagacgttg cgagtgtttt gggttattca aacactaaag
    55261 atgctttgtc gcggcatgta gatttggaag ataagatggg gtcgcgaatc acgacctcag
    55321 gtcaatcaag agagatgaca atcatcaacg aatctggttt gtacagttta atcttaaaat
    55381 caaaacttcc ctctgccaaa aaatttaaac gttgggtaac aagtgaagtg ttgccagcaa
    55441 ttagaaaaca tggaggttat ctaactccag aaaaagtaga agaagctttg cttaatccag
    55501 atacaatcat tcaattagca actcaactaa aagaagaaag aactggaaga ttaatcgcag
    55561 aacaaaagat tgcagagtac gaacctaaaa tctcctattt agatagcata ttatcttcta
    55621 cagattcagt aacaattagt cagattgcag cagattatgg gatgtctcca caacagatga
    55681 ataaattact tcataaacta ggtattcaga aaaaagtcgg taaccaatgg ttattgtgca
    55741 aaaaacacat gaaccaagga tacacaaaat ctcatacaac tgagatcccg aaagccgatg
    55801 gtggcactaa aattgtaatg aataccaaat ggacacagaa agggcgtcta tttatctacg
    55861 aattactaaa aaaagaagga tattaccctc aaatggattt agaggaaatt ggttagaaag
    55921 gagttttagt atgactgaca ttgcagaaat cactcaacga gatagagaaa aaatcaaaga
    55981 atatgtcgaa agttcaaagt tcttaactta caccatgctt gctgaaagat ttggaattag
    56041 caaaagctac ttatctttaa ttttaaacgg taaaaagact tctgcagaag caaacagaat
    56101 tatagattcg attatcacta tgtacgaatt gtaaagggag gaacagctaa tgcaatatct
    56161 agaagcaaaa atcccaattc cagaaggcta tgtaattatc tcccaagtgg attatgagga
    56221 gttaaaaaaa gctgatgata ctggtagatg gatgacgttg ccagaagtgc tagaacggat
    56281 taacagaaaa tatgattggt ttacttctag agttttaaag aacccaagat atagaaatat
    56341 tatcgatatc gagaaaaaca aaaatggatt tgtctattat ccagttgaag gaagagacac
    56401 atatctattt ttaagaagta aaacacttga atttttagaa acaaattttt cggaaatctt
    56461 aaggaggcaa gcggatggga aaatttaata gagcgctagt gttcagcgca ccgctaatca
    56521 tctacgcttt aggactttgg ggaagcaggc aagcgttgat aggaacgatt gtttacatgg
    56581 tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa aagccaaccg
    56641 agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac tattactcag
    56701 tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc aacaattttg
    56761 tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag gagtttatta
    56821 aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt aggaggaata
    56881 tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc tttagcaatt
    56941 aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga aagttctaaa
    57001 ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt ataaatcata
    57061 tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat tccttttatt
    57121 tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca ttgaaaaatt
    57181 gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta taccaaaaag
    57241 gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg tgactacgag
    57301 cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag atattgatgc
    57361 actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg aaccagattc
    57421 atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct tgctggctat
    57481 ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag cattcaacga
    57541 aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata tgatgatcta
    57601 tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag atactatgcg
    57661 aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag ttatgggagt
    57721 tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat tctcaaaatt
    57781 ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac ctcgtaagag
    57841 attcgatgat gctgtttcat ttattgaaat gtggttgcca gaaactgcga tccgtatgga
    57901 aattgatcaa ctgaacagac aacagagact ttttggtgat gacaatgaat agagctgaag
    57961 cgctaagaat agggacggca attgctaatt gctggtggaa atactataaa ccaatcatcc
    58021 taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag tgactccgcc
    58081 ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata tggaaaaaga
    58141 actttccact ctagatcaat atttgattga tcctgattgg ggcaagccga aaattgagga
    58201 aacaagtggt cgaaaaatca gacgaaatct tttgacgaat gaagaactag cttgggatca
    58261 agatgattta ggcaaccatg taactatttg ggatcatgtt tatcttatcc atctatcgaa
    58321 gcattcgaat aaacctgaat atatttacgt catcgaagat ggcttgattg atgcgctaga
    58381 agagtacgac agagataact tgattgatat ctcttattac ggaccaggta agaaatacat
    58441 tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac tttgaaaaac
    58501 tttttagtcg taagttaaat aaaattctca agaaaaaagg aaattttgat tatttatctt
    58561 gggctcacgc gtgggagatt atgaaaaaga atgatccaca ggcaacggta actattaatg
    58621 agtacaaaca ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt gaggaatata
    58681 aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg gttaaaggac
    58741 acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt gttaaaccaa
    58801 atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg gctctacacg
    58861 gactgggatt atatgtattt caaggggaag atattccaac accacctaga atcgatacaa
    58921 agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg ggtaaagata
    58981 tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg ctcttagctg
    59041 ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg gagcgagcaa
    59101 tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac cattaatcga
    59161 ttcatattca gcggttctga aaaagttcaa aggaaaagac ataagcgcaa ccatcaatga
    59221 ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg atcgagtcat
    59281 tgaaattcgt tttattgatc ctagacgttt caccgtacag caacgaaact tcatctatgc
    59341 gctgataggc gatattttta tcgatacagg catgccaacg gacttctgga aggaattctt
    59401 ctactttcgt tttgaaggtg tcacagggcg caaaataagc ctgaaagacg aatcgaatac
    59461 aactgtgagt gatgccaatg tcttagcaaa tatcatctta gatttcatct ttgaacatca
    59521 tattcctttc aaagaaggct atgagatttt acctgcgaat caagaatatt acttctacaa
    59581 atgcatcaca aaaagagtct gctgcatctg tggcaaaaca ggagctgaca tcgatcactt
    59641 tgacaaagcg ctaggaagac gaaagcgcaa agaagttgat catgcagagt acacatttgc
    59701 agcactctgc agaatccatc acacagagaa gcacaaaata ggtgtgatta atttcaaaaa
    59761 taaatatcaa atcaaaggga tcaaattaaa ccagaaaaca atcaaaaagt taaatattgg
    59821 aggggaaaaa tgacagaaca tcgaagttat tatgcgatta taccagccaa cgtaaggtac
    59881 gacaaaagac ttaaaccaaa tactaagttg ttatacggag agataacggc cttgtgtaat
    59941 gaaagaggct tttgttgggc aggcaatgag tactttgcag atttatatgg tgtgaataaa
    60001 gagaccatat cgcgatgggt aagtgatttg attaagtttg gatacttgaa tcgggaaatc
    60061 atttacaaag agggtaccaa tcaaataatc aataggtacc tacgaattaa tcaataccct
    60121 attgacgaaa aacgcaatac ccctattgac gaaaaagtca aagat
//
LOCUS       DACCXM010000004        56009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCXM010000004 DACCXM010000000
VERSION     DACCXM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.004"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..230
                     /locus_tag="IVY61_000187"
     CDS             <1..230
                     /locus_tag="IVY61_000187"
                     /inference="COORDINATES: protein motif:HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969330.1"
                     /translation="YQLVETQAPIGYELDATPIEFEIERSQTAVVELTKENRLTPGGV
                     VLTKIDDQSGEILQELFLSYRTEKEKRYKPV"
     gene            176..559
                     /locus_tag="IVY61_000188"
                     /pseudo
     CDS             176..559
                     /locus_tag="IVY61_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(983..1153)
                     /locus_tag="IVY61_000189"
     CDS             complement(983..1153)
                     /locus_tag="IVY61_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7969331.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1347..1526)
                     /locus_tag="IVY61_000190"
     CDS             complement(1347..1526)
                     /locus_tag="IVY61_000190"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969332.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1913..3025
                     /locus_tag="IVY61_000191"
     CDS             1913..3025
                     /locus_tag="IVY61_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7969333.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3103..3396)
                     /locus_tag="IVY61_000192"
     CDS             complement(3103..3396)
                     /locus_tag="IVY61_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP7969334.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3564..3878
                     /locus_tag="IVY61_000193"
     CDS             3564..3878
                     /locus_tag="IVY61_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP7969335.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3899..5878
                     /locus_tag="IVY61_000194"
     CDS             3899..5878
                     /locus_tag="IVY61_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7969336.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5891..6217
                     /locus_tag="IVY61_000195"
     CDS             5891..6217
                     /locus_tag="IVY61_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP7969337.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6233..6751
                     /locus_tag="IVY61_000196"
     CDS             6233..6751
                     /locus_tag="IVY61_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969338.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6751..8103
                     /gene="celB"
                     /locus_tag="IVY61_000197"
     CDS             6751..8103
                     /gene="celB"
                     /locus_tag="IVY61_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7969339.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8187..9692)
                     /locus_tag="IVY61_000198"
     CDS             complement(8187..9692)
                     /locus_tag="IVY61_000198"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP7969340.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9784..10374)
                     /locus_tag="IVY61_000199"
     CDS             complement(9784..10374)
                     /locus_tag="IVY61_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7969341.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10553..12199
                     /locus_tag="IVY61_000200"
     CDS             10553..12199
                     /locus_tag="IVY61_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7969342.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12248..12967
                     /locus_tag="IVY61_000201"
     CDS             12248..12967
                     /locus_tag="IVY61_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7969343.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13446..14393
                     /locus_tag="IVY61_000202"
     CDS             13446..14393
                     /locus_tag="IVY61_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7969344.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14405..15325
                     /locus_tag="IVY61_000203"
     CDS             14405..15325
                     /locus_tag="IVY61_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7969345.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15366..16823
                     /locus_tag="IVY61_000204"
     CDS             15366..16823
                     /locus_tag="IVY61_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7969346.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17168..17782
                     /locus_tag="IVY61_000205"
     CDS             17168..17782
                     /locus_tag="IVY61_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7969347.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17775..19499
                     /locus_tag="IVY61_000206"
     CDS             17775..19499
                     /locus_tag="IVY61_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7969348.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19496..20947
                     /locus_tag="IVY61_000207"
     CDS             19496..20947
                     /locus_tag="IVY61_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7969349.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21420..23573)
                     /locus_tag="IVY61_000208"
     CDS             complement(21420..23573)
                     /locus_tag="IVY61_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7969350.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23575..24621)
                     /locus_tag="IVY61_000209"
     CDS             complement(23575..24621)
                     /locus_tag="IVY61_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7969351.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24771..26060
                     /locus_tag="IVY61_000210"
     CDS             24771..26060
                     /locus_tag="IVY61_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP7969352.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26073..28775
                     /locus_tag="IVY61_000211"
     CDS             26073..28775
                     /locus_tag="IVY61_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7969353.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            28760..29617
                     /locus_tag="IVY61_000212"
     CDS             28760..29617
                     /locus_tag="IVY61_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7969354.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29688..30545)
                     /locus_tag="IVY61_000213"
     CDS             complement(29688..30545)
                     /locus_tag="IVY61_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7969355.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            30756..32927
                     /locus_tag="IVY61_000214"
     CDS             30756..32927
                     /locus_tag="IVY61_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7969356.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            32995..33336
                     /locus_tag="IVY61_000215"
     CDS             32995..33336
                     /locus_tag="IVY61_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7969357.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33612..35366)
                     /locus_tag="IVY61_000216"
     CDS             complement(33612..35366)
                     /locus_tag="IVY61_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969358.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35377..36213)
                     /locus_tag="IVY61_000217"
     CDS             complement(35377..36213)
                     /locus_tag="IVY61_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7969359.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36215..36898)
                     /locus_tag="IVY61_000218"
     CDS             complement(36215..36898)
                     /locus_tag="IVY61_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP7969360.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(37057..38451)
                     /gene="uxaC"
                     /locus_tag="IVY61_000219"
     CDS             complement(37057..38451)
                     /gene="uxaC"
                     /locus_tag="IVY61_000219"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7969361.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(38490..39989)
                     /locus_tag="IVY61_000220"
     CDS             complement(38490..39989)
                     /locus_tag="IVY61_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP7969362.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(39982..41403)
                     /locus_tag="IVY61_000221"
     CDS             complement(39982..41403)
                     /locus_tag="IVY61_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP7969363.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(41463..42455)
                     /locus_tag="IVY61_000222"
     CDS             complement(41463..42455)
                     /locus_tag="IVY61_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7969364.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            42687..44885
                     /locus_tag="IVY61_000223"
     CDS             42687..44885
                     /locus_tag="IVY61_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7969365.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(45343..46215)
                     /locus_tag="IVY61_000224"
     CDS             complement(45343..46215)
                     /locus_tag="IVY61_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7969366.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            46515..47488
                     /locus_tag="IVY61_000225"
                     /pseudo
     CDS             46515..47488
                     /locus_tag="IVY61_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            47510..48202
                     /locus_tag="IVY61_000226"
     CDS             47510..48202
                     /locus_tag="IVY61_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7969367.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            48168..49676
                     /locus_tag="IVY61_000227"
     CDS             48168..49676
                     /locus_tag="IVY61_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7969368.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            49750..50853
                     /locus_tag="IVY61_000228"
     CDS             49750..50853
                     /locus_tag="IVY61_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7969369.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            50856..52193
                     /locus_tag="IVY61_000229"
     CDS             50856..52193
                     /locus_tag="IVY61_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7969370.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            52180..53058
                     /locus_tag="IVY61_000230"
     CDS             52180..53058
                     /locus_tag="IVY61_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7969371.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            53055..53978
                     /locus_tag="IVY61_000231"
     CDS             53055..53978
                     /locus_tag="IVY61_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP7969372.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            53975..54385
                     /locus_tag="IVY61_000232"
     CDS             53975..54385
                     /locus_tag="IVY61_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7969373.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            54389..55957
                     /locus_tag="IVY61_000233"
     CDS             54389..55957
                     /locus_tag="IVY61_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP7969374.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT        18003 a         9860 c        11426 g        16720 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa tagggtatga attagatgcg acaccaatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt aacgaaagaa aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaaatc ctgcaggagc
      181 tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggtctaa ctacaggaga
      241 agatggaaaa ctggcaattg acggtttagc accaggagca tatcagctgg tagaaactca
      301 agcgccaata gggtatgaat tggatgcgac accagtaact ttcaagatcg aaaaagaaca
      361 agaagtggcg atctttttaa caaaagagaa tagaaaagtt gctgatagct cagatataag
      421 aggaaacaat ccaacaagca gtggaaataa gcatcttcca aaaactggag aaaccatcat
      481 tagccagttt atattgtcaa tattgggtgt gctacttgtt ttcatcagta taaaatttag
      541 aaagaaacga aatatatgag ctaaaataaa aaattgaata agcatagttt ttaaaatcaa
      601 ctagtaccca tcaattgaat ggaaactacc gattttagaa ggggtataca aacaaatatt
      661 agtatcggag tagagaaggt tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg
      721 cgttgagaag tgaaaaataa acctttgtag taatactagt taataaaaaa tatacaaaaa
      781 cactgcgaaa atcattcttg agtttcgcag tgttttttgc ctaatgactc aagtttattg
      841 ggtaatactt tgattttctt tctacaaata atcagcgatc agttgtaatt ttttctctag
      901 ttttaatggc tctttgtcaa taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct
      961 tcaaccaact cgtgaataaa ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc
     1021 gatcaatcac caatttcgca tttggtagaa catgtttggt gagctgaaag taggtggcgt
     1081 tcatgtctgt aactaagaat tctatttctt taggattagt gcagctaagg aaatagtttg
     1141 ttaatcgagg aattttttgt gtaggtaaat tttctcgatt aatttttctg tctcgccatc
     1201 cgcggaaata aaactcagtt tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc
     1261 aacactcaag acagaattct tttggaagac tttggtaaat agcttctcaa ctctttcaat
     1321 gtgcgaataa cggttgtcaa agatacctaa caacttttcg aaataaaaaa taacgacact
     1381 ttttcagtca gtaaagaagt gattttatgt ctgacatgat ttgaaatcga gtgccttgga
     1441 cggataaaat aactttgagc gttccaatgg cttcgacagt ttttacaggt atagcgctgc
     1501 ttttttaggc gtataaccat tggcatatga ttatattatt caaagtggac aatggtttcc
     1561 ttcttcttgt ttttcaccac cattaatttc ctatttccat ctaccacagc agaaccacaa
     1621 tttctacaga cacatggagc agggaagaga actgcagcaa cgatcaatgt ttttttcttc
     1681 tgtaaggttt cgtaagagac ctctgtaatc atcaaatttt tgtctattga tctcagaatt
     1741 tttttgatag aaccattcat aaagcatatc gttctctcag ttgtttattt tgtggtgatt
     1801 taatcataca agaaaacgat atgttttttt ataaaaacat gaataggaaa aatagttgac
     1861 aagaaaatga caacgccata tgatgttcca tagtatttac aacctgtcat agatgactgt
     1921 aaaaaataaa agtaaaaaga aaggacgaca acaggtggat ttttatcagt ctctacaact
     1981 agatccgttt attttaaaac aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat
     2041 gtatatttgt tcattgtttt tacgatcatt attcatcgta ttgtttgcca tttgttttat
     2101 cattataatt acaacattat ttgaatcgaa gcataaacca tacgctgtgg ttttattctg
     2161 tatgttgatg agtattcgtt ttgttgactt tggttataag atttcccatt cgatcatagg
     2221 tttagcaatc gtcatgttct cactattagt agcaccgtat gtccagctga tcaaatggtc
     2281 agttatgggc atgttgattc attttatttt actaagcagc atattaatgg caaccgctag
     2341 tgatcccaaa atggggaatg cttctttgta cggcttttca tatctcttta ttgtttattc
     2401 gttacctaaa gattcgttga acaagaattt ctttacacaa acaagtagct tattattttt
     2461 gtttttttgt tggttttcag ttctccttta cagaaaacat cgagaaaaga acaaagacaa
     2521 aagcttgttt aaagagattt ttttgaaagg catgtattcg caggaaaaaa tttggatgct
     2581 catctatgca tttggcatta gtcttttgat cgttgcagga gagtatgtcc cttttcagcg
     2641 attgatgtgg gcagggtttg cattttcttc tattgtatct agctatggat tgatgtcgat
     2701 tggtttcaaa gaacgagcag tagaccgaat cattggctca ttgattggat gtgttttatt
     2761 tattggtatc agccaattta ttccttttaa ctgggtcggt atattaggtg gtctagcttt
     2821 aggtgtttgt tctacctata gatataaaac agtttttaat tcttttggtg ctttggcaat
     2881 cactgccagt ttattcgggg ttccaggagc agtaacgatc cgcatttttg agaatatcct
     2941 tggggtatgt ttaggaatca tgtatattgg ggtgactgaa atacttatcc gaaaaataag
     3001 agaaaaacac ggtttgaatc attagatgag atcgaataaa aaataaggaa aaagtgaaga
     3061 tacacatgga tgataaattg ctatcattcc atgtgtatct ttttacggta aatcagtgaa
     3121 acgttcgact ttcattttta atttatactt tttacggagt tcgtttattt gagccatcat
     3181 ttcggatttg tgatgctttt cgattgcttc ttcatctttc caacggtcga tcagtaaaag
     3241 agattctgga tcttctagtg gaaagtaata gtcatatttc tcgtttcctt cttctgcacg
     3301 aatcgctttg acaatgtttc tttcgaccat ttcttctgca aattttctgg cttgtccatt
     3361 tgtacttgta tacaaaatat tcactgtgat actcactaaa tcccctcctg aaaaaattct
     3421 atcactagta tatcacggtg ccagaaatta ttattcaaga tatgatacac attttttccg
     3481 caatgctacg gaaatatata ttttgaatag aaaacgctta caagatatgc ttggtttata
     3541 aaaataaaag gagtgattga tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt
     3601 catcctcgat gatggcctca aagacaacgg atttctttaa gcaaaaaaga gaagaggtac
     3661 tcgtagatgc agtttcagct acagaaggcg ataatatgat taagaatagt gattttgatt
     3721 tatttctgat cagcccgcaa acgacgatgt ttttggataa attcaaaaaa ttaggagaat
     3781 ctgttggaaa gcctgttgtt agtatccctt tccaagcgta tgtcccaatc cctacaggca
     3841 tccagaagtt agcagaggtg atcgaagaga acatatgatg gaataggagt ggatgataat
     3901 gaaacagaag gaaaaattgc tgttgagata tttgattgat cacaagaagg agtatgtcac
     3961 cagtcaacgt ttagcttctg aattgttatt atctgatcgg acaatccgta attacttgca
     4021 gcggatcaaa gagttagtag aaaaaaacgg aggtaagatc attgctaaac caggttatgg
     4081 ctaccaactg catattttac aaagactgac atttgacttg tttttgagtc ggcaagaaat
     4141 agtacctgga tatgctagag aaccgcaagg attttatgag tccgaagatc gacaaaagta
     4201 tatattgaac caattgcttt tggaagatgc cattcttttt atggatgatt tggcagaaga
     4261 gctttatatc agtcgttcca gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat
     4321 tccatattca ctaaaaatcg tttctaagca tggtcaaggg acatggatcg aaggggaaga
     4381 aagaaatcga cggcatttta tcatggatac tttttttgga aaccattatg gaaattcatt
     4441 gaaagaatat ttagggcaaa gccgtttttt cccagacatc agttttgaag agctagtcat
     4501 tatcattctc gatgaaacaa gagaagctaa gttaaaagtt tcggatttta tcatacagaa
     4561 tctgacttta cacttggctt tggggatcaa acggctccga gaagggtttg aaatcaagga
     4621 attaggcatc gaacaagaag tttctaagcg agtagagtat caagtggcgc agcggatcgt
     4681 tcaacgtatt gaggtaatcg caggtgtatc gtttcccgaa gaagaaattg cttatttgac
     4741 attacatctc atggctaagt cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa
     4801 ggaattagcg aaagttctga aaagattgtc tcaggaattt catttttcat tagtagagga
     4861 tctccagctt ttcaatggac tactcgatca tttgtcaccg atgatgattc gattgagccg
     4921 aggtattcgc attgagaacc cattgactga ggagataaag aaagaaaatc ccaaagtctt
     4981 tgacgcagta aaaaggcatt tcagcaacat gccggctttg aaaaactata ccatcaatga
     5041 agatgaatgg gcctatttgg ctttgcatct catggcggca ttggaaaaag aaagagctgc
     5101 gcataaacta catgcattga ttatctgtgc aacgggttac ggcagtgccc aactattaaa
     5161 gaatcgcgta gtcagtgaat tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta
     5221 tgagatcaat gaaacaactt taaaaggaat cgacctgatt atttcttcta ttgatctttc
     5281 gacaatgttt ttcaaaattc cagttttaca tgtcagtatt tttttgaatg aagatgatgt
     5341 tcgtaaaata cgaaaggtag ttggagaatc tataccctat cattcctcag atagaccgat
     5401 accagcattt tctctacagc aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt
     5461 tttcaagact tatacgtacg aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa
     5521 gctgagcata aatgaagaag aaggatatat aagagaaatg cgaaagcaga tccagcaacg
     5581 tgaaaatatg ggacaaatca tattcagtga tacagtggca gtcccacatc ccgctgttcc
     5641 agttggaatc gaaacaaagg tagccgttgc attgattcct tctgggatgc agtgggatca
     5701 atatcaagaa attcattttg tttttttagt ttcgccttct tatgtcgaaa atgaaggaat
     5761 tacggtcgtg actaaagcaa tcgttaggtt agttgaccag ctaaaagtac agcaggaaat
     5821 cttagctgaa cctatttttg agaatttcag cagtaaattt atcaaactga tcgaataagg
     5881 aggaaaacag atggctgaaa aaataaacag tgaaacacta caagtgactt cttttgatat
     5941 cattttccat agcggaaatg cacggacact gatacatgaa gcatttcgct cgatgcgtaa
     6001 agagatgttt gaagaagtag aagagaagtt gcgacaagca aatgaagaga ttcttcatgc
     6061 gcatcagtca caaactcaac ttctgaaaga atatgccagc gggacaaaaa tagaaatgga
     6121 aattatcatg gtccatgcac aagatcatct gatgacgacg atgacattat tggaagttgc
     6181 acaggaaatg aattatttgt atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga
     6241 tacagtaaaa aggaaaatcg aattagaaaa aaagcaactt agtttgaaaa acatgtattt
     6301 caatcgttat ctaatcgtac gttacctgac tgcctttttc tttttcatga acatgcaatg
     6361 gatgattctg cttctatcag ctaaaagttt aggaagtagt ttaccgatga ttctgatttt
     6421 agctattttg cctgcagtag gagaacaagt aaagctttat cgaaaacacc aaacgaacgt
     6481 tccatggaca aagtggtatt ttttgttcca gagtgtcttt aatttattag tgattccagt
     6541 gttgtttacc tcaggattta cattcttgta tccttttatg gcaaataaca atggaggaca
     6601 gttatttgtt ttcattcttg ttgtaagcgg tatttttgcc agtgcattta tacagtatcg
     6661 tttaaagaaa atttcactca accaagacca acagtatatc cgtattaaac aatatgaaaa
     6721 agcgttatat ttaggaaagg agaataacta atgtttgatt ttttgcagaa atacctgatg
     6781 ggacctatgg ggaaaattgc gcaacttaaa atcgttcggg ctgtcatggc agcggggatg
     6841 gcttctattc ctttcacgat tgtaggctcg atgtttttgg ttttaaatat tttgccatta
     6901 ccgttcccat ttttagaagg attttttaat gcaacttttt tcaaagttag tgacttgtac
     6961 atgatcgtca atacaatgac gatggggatt ttatctattt actttgccat tgtgtttgct
     7021 tatgaattaa cgtcaattga acgagatgaa caaaaattaa atgttaatcc gctcacgggg
     7081 gcattactat ctgtttttgc attctttatg tgtatccctg aattgatcat gactgatggt
     7141 aaaatcagct tgattgcttc tatcaatgag acagaaactg tagtaaatgg tattaggatg
     7201 ggctcttttg tcgaacgttt aggaacttca ggaatcttta cagctattat tatgtcgatt
     7261 attgcggtcg aattgtattg tatgtgtgtc aagagaaatt gggtcataaa aatgccagat
     7321 gttgttccgc caggagtttc tcgttctttc actgctctga tcccaacatt tgtcattgcg
     7381 tttgtggtga tgatcatcaa tggattatta gttgcactag gaacggatat cttcaaaatg
     7441 attgctatcc catttgggtt tgttacgcag ttgacgaata catggcttgg tgtcatggtg
     7501 atttatttct tgatccatgc attgtggatc gtagggatcc atggagcgaa tatcatcact
     7561 tctttcttga ccccgatcgt attagctaat atggcagcta acgcacaagg cgcaaattat
     7621 ccgttggcag gagaattcaa caattcttat gtaaccgttg gtggatcagg tgcgacttta
     7681 ggattgatca tctttattgc attcctcgcg aaatctgatc agctgaaagt gttaggaaaa
     7741 gcctcacttg taccaggatt attcaatatc aatgaaccaa tcattttcgg gatgccaatc
     7801 gtctacaatc cctatttagc ggttccgttt ttcttagctc cgatggcttc tgctacactc
     7861 gcttattttg ccattcggct ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct
     7921 tcaccagtcg ggatcggagc atttgttggt agtggtggtg attggaaagc tgctgtgtta
     7981 gcagtcattt gtgcaatcat cgcattcatt atctggttcc catttatcaa attctatgat
     8041 aataaattat taaatgaaga aagagaaaag gcagcagaat tagctagtga aaagttaaaa
     8101 tgatttctaa gaaaaaagga actgtgacaa aaaatcacag tccctttttt tcttagaaat
     8161 gggaaaccac tagtatataa ataccattat tcttcaatca accgcttcac gattgcataa
     8221 tccccttcat aaggttcatc tacaccattg attttttgat agggatccac gccttttccc
     8281 gcaataacga ctgaatcttg cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga
     8341 cgatccattt cataatgaac gatcaaatct ggattagtaa ttgcttcatt gatttcttcc
     8401 gcgatttttt tcggatcttc aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct
     8461 gtttctgata gcacttttcc aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg
     8521 cctaatacaa caattacacg gccatctgga tgttcatttt tggcaaattc cagcaatgtt
     8581 ttcaagctta gataattatg tgcatagtcc acatagacat gcgctccatt ggattggatc
     8641 aattgatcca ttcggcctgg tacacgagct tcttttaatc cgtcttgtgc atcttgactg
     8701 gaggcacctg ttagtgctgc ggcaatgatc gcactggctg cattttcttg gttgaatcct
     8761 ccagccagca aaatatcata agcagtatcg cagatagcta agcgatcttc atgagaagat
     8821 aaagtaaaac cgtgagtacc tttatctgaa cggatcactt gataattgct ttcttccgaa
     8881 cgaccataag tgatcgttgg aatctggaat agatcggctg tttccctaag taaatcaaaa
     8941 tagtcacttt catgatttaa aacaactgtt tttgaattca agatcaattg tcgcttgcaa
     9001 tagaaatagt catcaaatgt cggatgttcg atcggactga tatggtcagg cgagatattc
     9061 aagaagattc cgacatctaa agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa
     9121 gaaacttcca tcactagatg agtcatacca ttttcaactg cttctgacat catacgatat
     9181 aaatctaatg actcaggcgt tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt
     9241 gtattcatag tagaaagcaa tgctgttttt ttatttgttg tgtgatccaa gattgctttt
     9301 gtaaagtacg cagcagttgt tttacctttt gttccagtga atccgatcac tttcaattta
     9361 ttttgcggat agtcgtaaaa agccatagca agaagcgcca ttgctttacg gatatccgtt
     9421 acgatgattc ctactgctga attttcgtat ggttgttctg atacataata ttgaataccc
     9481 tgttcgattg ctgaatccaa atattcttct ttgaaattca ttcctttaca gaaaaacaaa
     9541 gtagaagcat cagcttttct tgaatcataa cttaactgaa gaaattcagc tttttctggt
     9601 acatcaagat gccagcctct tgttgaaaca aattctttta atagattttc ttttaatagt
     9661 aaatgacgga tgtcttctaa agagaagctc attgattgat ccctcgcttt attcaatttc
     9721 ttggtttatt atagcacact ctttttaata tatcgtttgt ttccgctatg acaaaatcca
     9781 gctttattca aattttcttg acacagggga caagcggatg agtattttca taaacaatga
     9841 actgactgta atagatagga gaacgaatag tgtctccaac aatccaaaaa cataataatt
     9901 caaaaagacg ataagactat tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa
     9961 tttgtggaaa ataatcggcg gtatcgttat accgccattt gaggccccga ctgtgtacaa
    10021 tgtgtaaacc ccaagactga acaaaagact tcctgaaatc agtgaaaaga aatgtgagga
    10081 gatcatttcc acttttggaa caatctctaa aacaacagga aaaagcagac ttcccgctac
    10141 agtcttaaaa aatactgatt ttcctaatag gacagcggct agaagcagca tcataagatt
    10201 gatgctccaa acagtaactg agcgttggat agaaaaggcc gtttcaacta aatgtccgat
    10261 ggcgcccact ccggcggaag ctatatcgtg agggagcaag aaccaattca atccaactgt
    10321 gataaagaaa agacctaaaa atataacggg tagttttttt atttcttgtg tcattgatat
    10381 ctcctataaa tgttagccat aaaaggctgt atatttcttt actttaccaa aaaaacgaaa
    10441 ccgaattcaa aaaaacaatt gttcttataa aagttatata aaatcgattg ccagtttctg
    10501 ttttaccaat taaaatgata gtaatgtttt gtagaaagtt ggaatctgac cgatggataa
    10561 tcaaagaaaa ccagcattag atcaattaac accgcaagaa aaaatggcac gaggctctgc
    10621 ttggatgacc gccagtaata tcatttcaag attgctaggc gctgtctata tcattccctg
    10681 gtatgcttgg atgggcgaaa atgctaaggc tgccaatgga ttattcaata tgggatacaa
    10741 tatatatgca ttgtttctac tgatctctac agcaggaata cctgctgcga ttgcaaaaca
    10801 aactgcacgc tataattcat tgaatgaata tggcacttca cgacgattat ttatccgtgc
    10861 attacagatg atgggcgggc taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc
    10921 ttggctagct catgcttctg gtggcggaga agaactgatt ccaacgatgc gttctttgag
    10981 tatcgctgta ttggtttttc cttgtatgag tgtgatacga gggtattttc aaggcaatca
    11041 agaaatgatg ccttatgcgc tgtcgcagat tgtagaacag gtggcacgta ttttttatat
    11101 gctattggct acattcatca tcatgaaagt catggatggt gactatgtga cggcggtcac
    11161 gcaatcaact ttcgcggcat ttatcggtat gctagcaagt attcttgtcc ttttgtattt
    11221 tttgaagaaa catcaagcgt atacatcggc gtttattcac tacagcgaaa acaaaataaa
    11281 tattgcaacg aaggaattgt tgttagatac cgttaaagaa gcgattccgt ttattattgt
    11341 gggttcaggg gtaacgatct tcaaattagt cgatcagttc acttttatga agatcatgtc
    11401 tgcatccacc gagtattcca atgcacagtt actggatctt ttttcgattt ttagtgccaa
    11461 tccagataag ttgacgatgg tagttattgc attggcaaca tcgatcgcgt ctactgggtt
    11521 accgttgatt acagaagctg ttacgttgaa agatcgagta ggtctggcaa aattgacaag
    11581 cggaaattta cagttgtttt cattttttat gtttccggcg acatttggtg ttatgttact
    11641 tgcctatccg ctaaatacat tattttatac accggattct ctaggaagta atgtactgat
    11701 ccaagcaagt tttgtaggac tcttcctagg actatatatg ctagtttcca acatgctgca
    11761 aggaatgttt gaaaacaagg cggcaattaa ttatttggtt attgggtttc tagtgaaact
    11821 ccttcttcaa tacccagcca ttcgtttatt tgaagtatac ggccctttat tggcgacaat
    11881 gatcggattt gctgtgtctt gtaccttgat cttaaagaga atccatgaag tagcccgctt
    11941 taaaccaagt tttgtctggc gcaggacatt gctgatcttt attcttacct tgattatgct
    12001 tttggcttcg tggatcacaa agatgatttt tggtgctttt ttaaacgaag atagcaaatt
    12061 ccagtctcta ttgttgattc ttgtagtagc aggtataggg gctttagtgt atggttattt
    12121 agctttgaaa atccgcttgg cagaaagact actaggacca ggaatagcgc gtttacgaca
    12181 taaattaaaa ataaaataag tgaatcagaa gagctagatt gatgctcttt tgaaatgggg
    12241 gaaacgcatg cgtttagata agtttttagc agatatggga cttggcagcc gcaaagaagt
    12301 caaaggattg atcaaaaaag gaacgatcga agtcaatgga caagtggtga aaagcgataa
    12361 agcgcaagta aatgaattcg aagatgcggt tacttattta ggagaaatca ttacgtatca
    12421 aaaagatttt tactacattt tgcataaacc tgcaggagtc atttctgcta ctcaggataa
    12481 tcatgatcaa accgtaatcg atttattgga agaccaggat tatcgggaag atctttttcc
    12541 agtcggccga ttagataaag acacagaggg cctcttgatt ttaacgaatg acggggtgtt
    12601 tgcccatcag ttactatcac ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag
    12661 cggtataatg acagaagaag atatccgttt atttgcagaa ggactgatta ttgatggcga
    12721 tgagcagaca ttgccggcaa aactccaaat tgatcaagta gatcaagaga cagaaacttc
    12781 aattatccgt ctgattttgc atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc
    12841 agtaggaaaa gaagtcttat atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa
    12901 agatttgaat aaagggcaat atcgacccat gaccaaagaa gagctagaac aagtgagaga
    12961 aaaataattg aatggatagc taaaagaata agcagagtag ttgtagcgcc atctgaaaag
    13021 cagacttgtc cgtctgactt tcggatggct tttttgtgaa atttcggctt atttccgtag
    13081 aagctacttc tggaacaccg tttgttcgga ataagggact gtgacaagac ttttgttaca
    13141 gtccccattg ttccttgtga ctcagagtta gatagtaagg acacgatctc cctaatactc
    13201 aggccaaaac gctgaattca caacctctga taaacggtgt tcaatcagct gaccctcggt
    13261 attagctcaa aaaacggcaa aatatgagga actattttcc cgtttttttc tctaatactc
    13321 aggccaaaac gctgatttca caacctctcc tatactttgt ttcattttcc ctatactttg
    13381 tttatttatt ctcattattg aaagcgctat aattacgttg cgaatgaatc gaaaggggtt
    13441 tttagatgaa aaaacgaaag gggttcaatc atttttttat gaatctatgg cgttataaag
    13501 cacttgtcct gatggctgta ccgggtatgc tttggatgat ttttttcttt tacatacctg
    13561 taatggcgaa tgtcgtagca ttcaaaaatt ttcatatttc agcagatggg ttcttggcta
    13621 gcttgaggga aagtccttgg gtgggttggg aaaatttcaa atttttattt tcttcggatc
    13681 aagcatttct catcactaaa aatacaattt tatataatgt agcatttatc cttttgaatc
    13741 tgctgatctc agtcgtattt gcaatcatta tgagcgagtt gagaaataaa cgactcgtta
    13801 aagtttatca aacgatgtcc ttactacctt attttctatc ttgggtcatt atcagttact
    13861 ttgtttacgc ttttttaagt cctgacaaag gaatattcaa tcagtggatc actggacatg
    13921 gaggagaagc tgtcaattgg tataatgaac cgaaatattg gccgtttatc ttggtgttca
    13981 ttggtacatg gaaagggatt ggttataaca gcattattta ctttgcatct gttatgggaa
    14041 tcgatccgac ttattatgaa gctgctatgg ttgatggagc tagcaaatgg cagcagatca
    14101 aacatgtgac gataccacaa cttattccac taatgacgat tttaacgatt ttagccgtag
    14161 gaaacatttt ccgagctgat tttggcctgt tttataacat tcctaggaat tccggtgcat
    14221 tatatgatgt gactcaagtt ttggatacgt atatctacaa tggcctgaca tccacaggag
    14281 attttggtat gacggctgct gcaggacttt accaatcagt tgtcggcttt gtcttgttga
    14341 tgatcacaaa tacgatcgcc cgacgttttg atagcgattc tgcattattc taaggaggaa
    14401 aaaaatgaaa aaaaagaaag tggagagagt ccaagtccgc tctttcaata agacggctaa
    14461 ttttttctct aatataatga ttgcactctt tgctatttcc tgtattttgc catttatatt
    14521 tgttatcgcc atctctttta ccgatgagtc catattgtcg actcacgggt acagtttttg
    14581 gccagaaaag ttcagtatat ttggttatac gtacttgttc gaccagatgc aggataaaat
    14641 tttccaagcg cttttcgtca ctgtacttgt tactgtttta ggtacactga tcaatagcac
    14701 ggctacttct ttatatgctt atgcgatttc acggtcaaac tttccattcc gtcgtttttt
    14761 taccgttttt tgtttgatta cgatgttgtt ctcaccaggg atggtggcca attatcttgt
    14821 tatgacaaat cttcttcagt taaaagatac gatttgggca ttgatcttgc caatggctgt
    14881 cagtcccttc aatattattg tgatgcgcac attctttaaa cgttcggtct ctgattcgat
    14941 tattgaatcg gcacggatcg atggagctag cgaactgcga atatttgttc agatcgtttt
    15001 accgttagct gttccaggta ttgcgacaat cagtttattt gccgcattgg gttactggaa
    15061 tgactggttc aatgctttac tatacatcca agatgataag ctagttccac tacagtatct
    15121 tttgatgaaa atccaaagta atattcaata tctgacacaa aattcaggag caggaagcca
    15181 attgacaggc ggattagcct cagttccagg agaatctgcc agaatggcga tcgtagtcat
    15241 ctcgacgttg cctattgcga tcagctatcc atttttccag aagcattttg tcaaaggtct
    15301 aacaatcggc ggagtaaaag aataaagaaa caaaaatata aatcataaaa ttaggaggaa
    15361 gaaaaatgaa gttatggaag aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa
    15421 cattagcagc atgcggaggt tctggttcta aagaccaggc agaagctagc aattcggaca
    15481 ctttgcagat gtatcaaatc ggagataaac cagataattt cgatcaattg atggaagtag
    15541 caaacaaacg aatcgaaaaa gagatcggtg taaaagtcaa tatcaattac attggctggg
    15601 gcgattacga aaagaaaatg aacgtcatca tttcttccgg ggaaaattat gatatcgcct
    15661 ttgcgaacaa ctatgtttcc aatgcccaaa aaggagcctt tgctgatttg actgaattag
    15721 cgcctaagta cgctgaaaaa gcttataatg atttagatga agcatatatt aaaggaaatc
    15781 tggtcaatgg aaaattatat gctttcccag tcaatggaaa tgtctttgct caacaagtac
    15841 ttacttttaa caaagcttta ctagataaat acgatctttc aattgatgga atccaatctt
    15901 acgctgatgc agaaaaagtc ttgaaggaat tccatgaaaa agaaccaaac acggcagctt
    15961 ttgccattgg acaaggcttc aaagtccaag ggaactttga ttatccttta gggaatacgt
    16021 taccatttgc tattgatctg gatggagatg attcgaaaat catcaaccaa tacgataatg
    16081 aaacgtatat cggactgtta cgtacaatgc atagctggta caaacaagga ctcattccaa
    16141 gtgacgctgc aacgagcaat aaggattatc cactagaagg aaatacttgg ctaatgcgtg
    16201 gagaaacaca aggaccgtat gattatggcg atacaatttt gacaaatgca gcaaaacaag
    16261 aacttgtctc aaaagcaatt actgttccac taaaatctac agctcaagca caaatggcaa
    16321 attttgtcgt atcaaatact tcaaaaaata aagaaaaatc agtagaactt ctagggctgc
    16381 ttaacagtga tccagaatta ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg
    16441 aaaaagtcga tggttcagat gataaaatca aattgctaga tggttatcag ccaaatacac
    16501 atatgtctgc ttggaataca ggaaacaaca agattctata tacacaagag tctattacgg
    16561 atgacatgat tgccaaacgt gatcaatcga ttgctgatgc agaaacttca ccgattttag
    16621 gattcagctt caacactgac agtgtcaaaa cagaattaag taatatttct aatgtcatga
    16681 atcaatattt agatggattg aatacaggga ctgttgatcc agatgaaaca ttaccgaaat
    16741 tgaaagacgc tttggacaag gctggctatg ataaggtatt gaaagaaatg caaaaacaat
    16801 atgatgagtt ccgacaagaa taataatgaa taaagacaaa ggggttgtga caaaagacgt
    16861 gtcacaacct cttgttccga ataaacggtg ttcaaaagcc agtttctcgg tattaagcta
    16921 aatatatgta aaaatgtgga aagccatatt cacaaaattt ttcttactac tcgaaactaa
    16981 gcggctattt cacaaactct tggtaaacgg tgttcaatca gctaaccctc ggtattcgct
    17041 cacaaaacga caaaatatga ggaactattt ttccgtttgt ttctctaata atcaggtcaa
    17101 ggtgctgatt gcccaacctt tttttgtata atgagaaagt ataaacttaa tgtttggagg
    17161 agaagatatg ttagggaagg ttttggaaga gttatttatc cggatctggg tcattataaa
    17221 actaaccttg tatttctgga tatatacatt tgccggcggg atcatctttg gattaggtgc
    17281 ggcatggaaa acagtcaatg aactatttta tttgtacgga ttcgagtata aagaaatcac
    17341 aataaaaaga ggctggaata tatataaaag aaatttttta agaggcaatc tgctatttag
    17401 tttattttta tctggtactg ctttattgag ttacaattta tatttatctg ttcaaatcaa
    17461 agggctcatt tttttagcta ttgattttat tttacttttt gccttgctct gcctatttgc
    17521 tacttatcaa tacagtatga ttttagatag tgaatatacg atctctgttc ctaatctgct
    17581 aaagctaagt tttatttcgg ttttttccag cttttcgtct tttctaaaaa taattattgg
    17641 tagcggtata attttaggcg tgacttggca attcaaagga ttgatcctgt ttggtgtcat
    17701 tggtcttttg acggtttgga atggtacgat gacaacacat tggcgtgaag aattagataa
    17761 acagttggaa agttatgaat aagtttcgga ttctattttc aaaaggaacc ttactaaatc
    17821 gtctgttgcg gaaatacgcg gttatcatga tcggtgtaac gatgactgct accgttattt
    17881 ttagtatcca tacttgggaa caaaatcaaa aacaggcaga aaacatgaca agtgatgccg
    17941 ttcaaagcac aagcagaatg ctgaatgata aaacaacctt gagtaggatc atcaaaaacc
    18001 agcttgtagg gaattccgaa aagatcgaaa atgtgacgac ctacttaaca aaacccatag
    18061 atcagtatct gatgtatgta tacgaacagc aaaacagtac cgatgaatta gtttctttcc
    18121 caaatcaaat caaagatctc tacgcaaatt atgaagaact aagtgcgata tatatcgtgt
    18181 tgaatcaact accggagtat tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa
    18241 aggggacacc aaaattacta gacgcctttt atatcaagtt gcctatcaca caaggaggag
    18301 ctgagtcagg aagtattttt attggttttc aaaagaaaga actagatatg attttagaaa
    18361 atctaacgtc attcaatgga ctctctttat acatgatttc tggaacgaca aatcgactat
    18421 acacattcca tgataataaa atagctaaag aaacgtttga aagacaagaa atgatcatca
    18481 cagagagtct gaagaaaaat tcacaattgc cgattgacga tctggaaaaa aacaattttg
    18541 ttcaacacca agaactgtct gatgattatc gtatattagc tgtactagat aaaaaaagtg
    18601 taaaaatcga aacgggaaaa aatcttgccc cgctgatcat tggtatcatt attttagaca
    18661 atatactgct tatctttctt tataaaacat tcaaacgata ttcacagcaa gtatctatcg
    18721 tcatgcaggc catggatcaa acagcagcag ggaatttaga gacgcggatc gacataacaa
    18781 acacggaata tgaactgaag gaactttcaa tcggtatcaa cgaaatgttg gatagcatca
    18841 atcagtttgt agaagacatc tacaaattag aaatcaaaca gcaagacgca catatgcggg
    18901 cgcttcaagc acagatcaat ccacactttt tatataatac attagaatat atccgaatgt
    18961 atgcaatcag cgaaggaagt gaagaattag cagatgttgt gtatgcgttt tccgctttgc
    19021 ttcgaaacaa tacgaatcaa gagaaaacga tcactttaaa agaagaactg gacttttgcg
    19081 aaaaatatgt ctatttgtat caaatgcgtt atcctaatcg agtagcctat cattttatga
    19141 tcgatcctga tttggaaaag atcgaagtac ctaaatttgt gatccagcca ttagttgaaa
    19201 attattttaa acacggtatt gattttactc gattcgataa tgcgctaagt gtcaaagtct
    19261 tgcaggaagg caaacgggta aggatcatca tcaaagataa tggaaaaggg atgactgaaa
    19321 aacggctaaa acagattgaa gaaaaactgt ctcatcctaa agtggaactt cacggttcta
    19381 tcggtctgca aaacgtgaat gaacgtttac gtgccagctt cggcagcagt tattatatgt
    19441 cgcttgagaa taatgagaca ggtggactga ccgtttctat tacattcaag gagggatgag
    19501 cgtgtatcaa gtgttattgg tagatgatga atacatgatt ttgaatgggt taaaaaagat
    19561 aattgattgg caaagtttag gctttcaaat agtagcaaca gcagagaatg ccaaagaagg
    19621 actagctgta cttgaacagc gacagattga tttagtagta acggatgtga caatgccaga
    19681 aataaatgga ctggaattta ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat
    19741 gattttatca ggataccaag aattcgatta tttgaaagga ggtatgcaat taggcgccgt
    19801 gaattatttg atgaaacccg tgaataagtt cgaattggtt gaaagcctca agaaaatcaa
    19861 aacacggtta gatcagcaaa atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt
    19921 attcagtcaa tggttgaatg aagaattgga tgaaataagt gaagaagaaa taataggaca
    19981 aataagtgag aagcagcata ggatagtatt gattcaactg gcaagaactc atgatactct
    20041 tatcaataga tggttgcaag aacggcaaca aatcttttac tatcaacgaa attacggaga
    20101 ccgtgtattg atcacgttgt tgttggaggc agatgaagtt gatggcttct gttgttttgt
    20161 tcaagaaaac tttcctgatc aggaatggct gatcagtatc ggggaagaga cagattcttt
    20221 ggacaaaatc cctgaaacat tccaacaagc gaaagacaat ctacagttgc atcaatttta
    20281 tggagataaa gacgagcatg ttttttacgc agtacaagca gaaacaaaag atcaaaccat
    20341 cgatttttca agtttcagac gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat
    20401 gatcactgat ttttttgaac agtttcagtt agcagccatg atgccggaag atatacgata
    20461 ttccagtttt ttactgttta tggagattca acgggaattg attgatctgg aagatgagga
    20521 atatttacaa ggaatcgaaa aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct
    20581 acttctgtca ttcatcaaaa agcatcagag acagaaaaag tacagtgaca atgtggaaaa
    20641 agtaatcgta atcctgcacc agcattatca agaaccactt acactcaaag aagtgtcaga
    20701 aaacctgcat ttaaatgtca tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag
    20761 tttttcagct tatttgaacc atttacgtat ggaaaaagcg aaacaactgc ttttgcacag
    20821 taatcaaaac atcaatgaga tagcaagtga gattggctac aacaacacca cgtatttctc
    20881 taaattattc aagaaaatcg tcggcagatc ccctaaagag taccgagaaa acagtggaga
    20941 atcataaaaa aaaaagccaa tgtaatgaaa taacttataa aaaattgtct agaaatctaa
    21001 ttttgggctg ttcttattac ttgaggcaag gcagcttttt catgacctct cgataaacgg
    21061 agttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg gaaagccatt
    21121 ttcacaaaat ttttcttaat actcgaaact aaacagcttt ttcacgacct ctccactaaa
    21181 tggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta
    21241 ttttgccatt tgtctctcta atactcaggt cagaacgctg atttcacaac ctctcaataa
    21301 acggtgttca aaagcttgca tctgcggtaa taagaccaac caaacgaaaa atatgaggat
    21361 ctatttttgc ttggctgttc ttattacttg atgcaggaca gctttttcac aacctcttgt
    21421 taattatagt ttgataagga aaaaggcaaa tcttttgctt gatatttctt agggtgaggg
    21481 atgatcccta attggtaaga gatcgtgccg cctttcagca aatcttgatg ggtaaaatat
    21541 aatttcgtat attctctgtc catccgtttg attttatgga taaattgttg ctgcggccga
    21601 ttaggcgttg cttcaatcgt caattccttt ccatttgaca aaagcaaaac agccttgtca
    21661 attaatggca taccgataac atattcaccg gttccaggcg tgacaggata aaatcccaga
    21721 ctattgaaga tataccaagc ggccatactt ccattgtcct catctccagg gtaacccatt
    21781 ggagaatcgt cgaaggtctg agtcatcaac tgtttaagca gtggctgtgc catttcaggt
    21841 tgtcctatat aactgaacaa gtaaggatag tgaaaacttg gctggtttga aatagctaat
    21901 tgtccaaatt ctatcgcggc catctcactc atttcgtgga tctcaaaacc atatcctcct
    21961 gggtcaaacg ttgggggctg attgcacagc tcaatgagtt tttctttata tctctcactg
    22021 ccaccatatt ttccgatcag tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa
    22081 gcacttcctt ctgcataatc ttgtccccaa cgattagctg aaaatggttc tctgaacttg
    22141 ccatctttat cttttgaacg cataaatcct gactcatagt caaagatatt ttgatagttc
    22201 aatgattgct gctgatattt cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg
    22261 ctaatacaaa aatcgctata agcatagtct aatgtgtgat tgactgattc gtggtagtga
    22321 ttaggaacat agccatatgt caagtaatct tctgttcctt ggcgtccata atttggatgt
    22381 ttgctttgaa ccgtagcacc tttttccatt gccgttagaa attcaggcat caagtctcgt
    22441 cggatatctt ttacagcagc atctgcaatg accgcatcga tcaatgtccc aggcataagt
    22501 ccacgttcat caggagacag ccattttggc aaatagccac tttctctata gctgttcaaa
    22561 aaaccttcca gcatttcttc gtatttttct acagcaatca aagaataaag aggataaacc
    22621 gttttatacg tatcccaaaa cccattattt gtatagagaa caccaggttt tatagatcga
    22681 ctagtcgtat catagtggat tttttgccca ctaggatcaa tttcataaaa tgtttgtgga
    22741 aataaaaaca cccgatataa attatgataa aacgtccgca attgttgttg atcatggtgt
    22801 gtgagctgta tacgatcaaa ataggactgc cattgttctt gactgtgagc aaggtaagtc
    22861 tctttgcttt ggttttgttc tagttccagg ttataagctg cttgttccga gctgataaaa
    22921 gatgtcccaa atcggatagt ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg
    22981 ctatttttgt tggaaacagc taactcatca attgttaaag gatattcaaa tctcaatata
    23041 aaataaaaag aaaaatcagg atcttctgaa ccagcaaaat gtgtgatttt tccttccact
    23101 gtgtaaggat ctataacaaa gagctgatgg cgtcccggaa aggataaaag taaaccgctg
    23161 tcatttttgc tgtaatcaat agataagatt ccgccataca tgctaggtat caatgtagaa
    23221 cagatgccat aacgtaattg acagatcgac agatgagtcg ggcaaaatat actttcttct
    23281 gggcggtaag aactttgagc atggaaaagg gtgttctcct gtagttttcc attgatcggc
    23341 gtaaacagca tatgactgaa atcacccatc caaggacttg gttgatgggt aagacggtag
    23401 ccttgaaaaa cgcggtcatc aggatgaaac caccagctgc cgttttgatc cgtagtttgc
    23461 ggtgcaaaat aattcatccc aaaaggaacg ccagtataag gctggcagtt cccattagag
    23521 aaatttggct gattcgctgt tgcatgtcta gtatctattt gaccgatatt cattttattt
    23581 ctcctttgtt gattgtcttt tgatgaatga aactggaaat ttactatcgg atggtcgatc
    23641 ttttgtctct aaccaagtga ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc
    23701 ttgtgcgatg gttgtcagtg gcgggtcaac aagagctgct gcttggatat catcaaatcc
    23761 gataattgaa aaatcttcag ggatatgata accatgctgt ctcaagagat tcatggtctg
    23821 gatagcgaca acatcgtttt cacaaacaaa agcagttact tcatacttag tgatataaga
    23881 aagcaattca tctaatgtcg cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc
    23941 tttgtccaat gcttcaaggt aaccaaggta acgctgtcga acggattgtg gatgagtctg
    24001 cgtaccaaac aaataagcga tttttgaatg tccctgttcg atcaaataag aggcagcaag
    24061 cgcaccgcct tggaaattat cggaaagaac ggtaggaaaa ggtaactcat agattttttt
    24121 atctaatatg acgacaggga attctttcaa tgaaagctca aaaagtaagt caagatgttg
    24181 ttctgtatca agagcataat aaatcaatcc gtcaaattca ttgattagct cttttgctgt
    24241 tttattttgt agaaagtcaa gcgtcgtcat catgacatca atctgtttat cttgcatcac
    24301 agggttcaat cccttgctaa aatcaccaac agacaaatca gcgacatatg gaagaagaaa
    24361 cagaatccgt cgtgtttttg taggagaagc aagaatctgt tccggagaag cggatttaac
    24421 gaaactgcct ttcccgcgaa cgcgataaat aagtcctaat tgctctaatt cagtcaatgc
    24481 acgtttggaa gtgattctgc ttactttata ggtacgtgaa agttcttttt cagttggtag
    24541 ttggctgtct acaggtaatt caccagttaa aatagccgtt tgcaaatcat taaatattgt
    24601 ctggtacaat ggttgagtca tataaacacc tcttaatgat atatcatatt caaataatac
    24661 cataatggat gaaaaagtaa ataaaaagat attttttgaa agcgtagaca taattggctg
    24721 tatgaagtat aatctttaaa gatatatcaa atagaaagaa ggttttatta gtggtttata
    24781 aagatatacc aaaatcggtg cagaaattta tggaagatat cacacaaaaa tgtggggata
    24841 tccatgcgga ctgggcaaag aattttacag cagcatttgc caatactctg ttgacaacag
    24901 tcaaaaagca aaatgacgga accacctttt tattgacggg agacattcca gcgatgtggt
    24961 taagagactc tactgcacaa gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat
    25021 tagctgacat gatcagcggg ttagtcaaaa agcaattttt ttatatcaat atcgatccat
    25081 atgcgaatgc cttcaacgaa gaagctaacg gtgctggcca ccaaacagat catacaaaaa
    25141 tgaacgattg gatctgggaa agaaaatatg aaattgattc tttatgctac ccaatacagt
    25201 tggcctatct tctttacaaa aatacaggaa aaactgatca attcaatcaa tcttttattg
    25261 aaggtatcaa aaaaatcttg gacgtgttca aaacagagca agatcatgaa cactctcctt
    25321 atacatttgt acgagacacg acaagaaaag aagatacgct gatcaatgac gggcgaggtg
    25381 caaaaacagc ttttacagga atgacatggt ctggtttccg tcctagcgat gatgcttgcc
    25441 gttatggata tctggtacca tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag
    25501 aaatttttca aacgatactc gatgataaag aaattgtaca gcaaacacag aaattgaaga
    25561 aagagatcca agaggggatt cagaatcacg gacgaacggt taatcaaaaa gggcaaacga
    25621 tttatgcata tgaagtagat ggaatgggaa acgccacagt catggatgac agtaatgtgc
    25681 ccaatctgat cgccgcacct tacttgggat attgttctga acaagaggaa gattatctgc
    25741 aaacaagaag aacattgttg agtaaagaaa atccatattt ctatgaagga aagtacgcaa
    25801 aaggaattgg atcctcccat acgccagaaa aatacgtttg gccgattgca ttagctatgg
    25861 aaggaatgac aacgtcagac aaatctgaaa aagagcggat attgaattta cttgtagcaa
    25921 ctgacgcagg aacacacctt atgcacgaag gattcgatgt gaacaatcct gaaaattata
    25981 cgagagaatg gttttcttgg gcaaatatga tgttttgcga attagtcatg gattactttg
    26041 atattagagt agaaaagtag gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc
    26101 atagccactg ggatcgtgaa tggtacatgg cttacgaaca gcatcacatg cgactgattg
    26161 aattgataga cgatctacta gagctatttg aagttgatcc gtccttcaac agttttcatt
    26221 tggatgggca aacgattatt ttagatgact atcttcaagt aaggccagaa aaaagacagg
    26281 caatccaaca agcaatcaat gaaggaaaat tgagaattgg accattttat atcttgcaag
    26341 atgattttct tatcagctcg gaatcgaacg tgagaaatat gctgattggc atggaagaaa
    26401 gccgtaaatg gggaacccct gtcatgttag gctattttcc cgatacattt ggaaatatgg
    26461 gacagacgcc tcaattaatg aaacaagctg gtatttcagc agcagcgttt ggccgtggtg
    26521 taaaaccaat tggctttgat aatcaagttt tggaagcaga aaactattct tcccaatact
    26581 cagagatgtg gtggaaaggc cctgatcaaa cagcaatctt tggtctgcta tttgccaatt
    26641 ggtatagcaa cgggaatgag attccagtag aaaaagaagc agctcttgcc ttttggaagc
    26701 aaaaattagc ggatgcagaa caatatgcct ctacgaacca cttattgatg atgaatggtg
    26761 ttgatcatca accggtacaa aaagatatat caaaagcaat ccatctcgca aatgaactgt
    26821 ttccagatta cgagtttatt cattctaatt ttactgacta tctggaagca gttcaaaaag
    26881 atgtgccaga agacttaggc tcagtagaag gagaattgac aagtcaagaa acggatggct
    26941 ggtatacttt agccaataca gcatctgctc gggtttactt aaaacaatgg aataccaagg
    27001 tccagcgaca gctagaaaat attacagaac cacttgcgac gatggcttat gaagttagtg
    27061 gaaactatcc ccatgatcaa ttagattatg cttggaagac attaatgcaa aatcatcctc
    27121 acgacagtat ttgcggttgt tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg
    27181 aaaaagcaga tgaagttgga aagtatcttg cacaagattc cttagaacag ctgaccgcag
    27241 caatcgatac gaccggattc ccaaaagaca gctttccatt tgtcattgta aatactgcag
    27301 gaatggataa aactggtgaa gcagaaataa ccatcgaatt agaaagaaaa agattcgctg
    27361 aaggaatacc tgaacaacta tatcaagagt tagaaaattt gcctaaacgt aagtatcatg
    27421 tagaaacaaa aagtggggcc actataccag ctattttatc tgaagaaact gtccaatttg
    27481 gttatgattt gccgaaagac cgtttccgtg tgccatatat ggcaagaatg atcaaagtga
    27541 ctttaccatt agaaaacatg ccagcatttt cttgggaaac attcgcgctt gtagaagggg
    27601 aagcgaaagc agaagaaaag gaaacaatga ttcatcagag tggacgaatc atcgaaaacg
    27661 gccctctaca tttgacgatt gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca
    27721 aacgaaagtt gaataatctg catatttttg aagatatcgg cgatatagga aatgagtata
    27781 tctttaaaca acctttctgt gatcagccaa tcctatccag taacaaagaa aactctgaag
    27841 taaaagtatt agtagataca ccggaaatag caaaaataag tattctacag gaaatggaaa
    27901 tccctgtttc tgcagacgag cgtttagaaa aagaacagca gatggttgtg gaatttcgtt
    27961 atcgaaaagc agaacgatcc aaagaaaaac gaatccttca aataaaaaca atcatgacaa
    28021 tcagaaaaga tagtaaaaaa atcgactttg aaacgactct cgacaatcag atgaaagatc
    28081 atcgcttacg tgtcttgttt cctacaaagt tacatgtgga acatcacgaa gcagatagta
    28141 tttttgaagt ggtgaagcgc ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc
    28201 cgcaacatca gcaagcattt gtcaatattc atgatgaaga gtacggtgta acagtcggaa
    28261 actttggttt gaatgaatat gaagtaacag aagacggaca aatcgcagtg acgctacttc
    28321 gcagtgtggg agaactaggc gattggggat attttccaac accagaagca cagtgtctag
    28381 gtgagcatcg gttcaattac agcattgaac tacatggacc agaagaaaaa ttctctactt
    28441 atttgcatgc atacgcagca cagattcctt tttccactca acaaatcaaa catcatgaag
    28501 gaacattgat ttcaaaacaa caatatttaa cgataaaaag tgaaacgttt gcaattacag
    28561 ctttgaaacg cagcaagttt agtgataaag tagtagtaag aggtttcaat atgtctagtc
    28621 atttagaaaa attggaaata actaaagaca atggaaaaac tgtcatattg aatctgcttg
    28681 aagaaccaac gaagcaagcg gttgtcccaa taatccaacc ttatgagatt cggacaatcg
    28741 gattcgagga ggaaaacgaa tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg
    28801 tgcggtttca gatggtggag aaattttaga aaaattaagt atccctacca caacaccaga
    28861 agaaacattg gatcaggtat ttgaattttt tgatcgttat gaattgaagg gaatcgggat
    28921 tggctcattc ggcccgattg gtatcgacaa aacaaaagat aattacggat acgttttggc
    28981 aactcccaaa aaagggtggg cacaattcga ttttcttggc acaatcaagg aacgctacaa
    29041 tattcctgct gcttggacaa cggatgtcaa tgctgctgca tatggggaat tgaagcaagg
    29101 agcagcacaa ggaaaaaata gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg
    29161 tgtcgtgatc aatgaagaaa ttttttcagg gatcgcacat cctgaaatgg gacatatttg
    29221 ggtgaaacgc catccagatg accattatga aggtacttgt ccttatcata aagattgttt
    29281 agaaggatta gcagcaggac cgtctattga ggcacgcaca gggatcaaag ggcaagatct
    29341 gccgcaagat catcctgttt gggctattca ggcttattat attgctcaag cactgatcaa
    29401 ctatacgttg acgttagcac ctgaaaagat tattttaggc ggtggggtaa tgaatcagga
    29461 tcatctcctt caaaaaatcc gtcagcaatt tgttgaattg atgggtggtt atatggaaac
    29521 acctcaagta agtgaatata tcgttcgttg gggactgccg aatgaaagtg ggattatcgg
    29581 tagtttgctg ttagcagaac acgaacatca aaagtaagca gacaaattac acaaaaagaa
    29641 cagagtctta tcattatctg ctgataagac tctgttcttt tttatttcta ttcccataat
    29701 ccaggtaaag ctttaccgac tattttttct tgagtaaatg gatggatcat gtgcagttct
    29761 aatgcatgga gcatcagtcg ttcagctgtt ctattttggt agagagggtc tccaacgatc
    29821 ggatgtccct tatgagctaa atgaactctg atctggtgtg tacgtccggt ttccaaaaca
    29881 caatagactt gtgtttgttc agaaagtact ttgttgacag tgacatgtgt caaggcgctc
    29941 tgaccatttt tatcatctac gacccttttt ctccgatcat ggcgatctcg tccaatttta
    30001 ttggtgatgg tcatatcttt ggcaatttct ccccatacga tggcttgata tctccgatag
    30061 atcatttttt gttccaacat tcttccaagt atcggcaaga caaatggatt ttttgcaaat
    30121 aaaacggcac cgcttgtttc tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta
    30181 gtgtcgagat aagcagctaa atgattgagt aaggtactgt tttctgtcgg ttcatttgga
    30241 tgagtcttca ttcctgcagg cttatccact ataatcaaat gctcatcttc atataaaaca
    30301 cggatgtgct tcgcatctcc taattgaact tcttgataat tataatctgt ttcttcgaat
    30361 aacaatgtga tctggtcatt gccctttact tcatgatgga acaaagcagg ttgttgattg
    30421 atccaaacgt ttttacgtat acgcaaaaag tgtcgaacct ttctaggaac taaccattcc
    30481 ttttcaagca agtcgcgaat tgttgtttca ggatggttca taggtaaagt gatcgtgatt
    30541 ttcacaagtg tactcctttc agtgcaatcg cagactaatt gtaccatttt taaaacatat
    30601 ttaagaaaaa acttacattt ttctgttcat tcttatgtta ctctacttgc actaacgtaa
    30661 aaaaacatat tgaactacaa cttaagcagg aagaaaacac tctttcacta tggtaaaata
    30721 agacgattga atcaaataga caggagaaga agcaaatgga ttttcaaagc attttagaaa
    30781 agataaaagc tgctttgatc agtttttggc gttggatcag accatatctc ggacaattcc
    30841 atcgatggcg aaagcgcatc tggaaaaaat atcatgtgaa caaattgatt ttgctgattg
    30901 gtttgatctg tgtcttagtg acaagtatct acctctttat tttagcaaaa caagcaaatg
    30961 tcgaaacgtt aaaatcagga ttaagccaat cgacagtcat ttatgataag aaaaacgaag
    31021 aagctggcac actgtacgcc caaaaaggaa catatgtaga attaaacgcg atttctccct
    31081 atatccaaga tgcagtaatc tcgacagaag ataggaattt ttacgagcat cacgggtttg
    31141 atatcaaagg gattgcccgt gcagcagtcc gaatggtctt gtctggcgga acatcagggg
    31201 gcggaggaag tacgatcact cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa
    31261 cgtttgatcg aaaagcgaag gaattatttt tggcaatcga gatcgaaaag aagtacagca
    31321 aagaagaaat cttgaccatg tatcttaaca atgcctattt tggcaacggg gtatggggtg
    31381 tccaagacgc ttccagaaag tattttggag tagatgcaac gaatgttacg ctgtctgagg
    31441 ctgctacttt ggcaggaatg ctgaaaggtc ctggtattta taatccgatc gatcacccaa
    31501 aaaatgcgaa tgatcggaaa aacacagtac taagtgtaat ggaagaaaac ggaaaaatca
    31561 caaaagaaca agaacaagca gaaagccaga cagatatcag cgcgtatctt aatgacacat
    31621 acgataattc agattctggt tatcgttatc cttattattt tgatgcagtg atcgatgaag
    31681 cagttaacga gtatggtctt gatgaagaag atgtcatgaa taaaggctat aaaatctata
    31741 cagctttaga tcaggattat caaaagcaat tagaagctac ttatcaaaac agtgcattat
    31801 tcccagcaaa tgcttcagat ggtgccatgg tccaatctgc ttcagtagct ttagatccga
    31861 atacaggtgg tgtacaagca ctagtaggac gaagaggaga tcatgtcttt agaggattca
    31921 gctttgctac acagatgcgt cgttcaccag gatcaacgat caaaccatta agcgtatata
    31981 caccagcact ggaagctgga tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt
    32041 attacgatgc gcataatttt gacgggacat accaaggaga agtaccgatg tatcaagctg
    32101 ttgcacaaag tttgaatttg cctactgtat ggcttttgca tgagatcggt ttacaaaaag
    32161 gatatgataa agcagaagaa ttcggcctgc ctttagcaaa atctgataag tattatggct
    32221 tagcccttgg tggactggaa aaaggagtat ctccattgat catggcgggt gcttacagtg
    32281 catttgccaa tgatggtcag atgtatacgc cgcatcttat cacgaaaatc gttgattcga
    32341 ctggagcggt gatcgtcgat aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa
    32401 cagccgatga aatgaccagc atgcttcttg gtacattttc aaatggaaca gcggctgctg
    32461 cgaatcctgc aggttatacg atagcaggga aaacaggaac cacagaaact aatttcgatg
    32521 ccacaaaagc aaatgaccaa tggatgatcg gttacacgcc agacgtagtg atttcgacat
    32581 ggatgggtta cgaagaatcc agcaagctgc actatttgga agggacaagt ggaaatgttg
    32641 tcgggaaagt cttcaaatca gcggcagaag gcatccttcc gtatacttca aaaacaggat
    32701 ttagtgtagc agatgcctat gccacaggag gacaagtggt agctgcagat caagttggaa
    32761 acacaggaaa ttctaatgga gagtctggaa attggcaaga taacttgaat caatatggtg
    32821 aacaagcaaa agaagggctg aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa
    32881 ttggtgaagc ggctaaagac ctctggcgga aatatcaagg agagtagccc ctttgaaaac
    32941 gaataacccc atgttacaat agagagtaac tactaaaaga aaagagggca taacatgtca
    33001 aacatctacg atagcgcgaa tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa
    33061 gcgttgaaag aagcgtacga gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa
    33121 gacttccaag cgatgcaaat cgaattacaa gaaaaacaaa tgagtggaca agagttcagc
    33181 gacgaagatg caatgaaagc acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc
    33241 aatgacttga tgcaaaaaga gcaaggcttc agtacaatca tcaatgactt gaaccgtatc
    33301 atcatgacac cagttcgcga cctttacagc gaataaaact attttatgaa acaacagagg
    33361 gtgtgaaaga gacgttcggc cttgagcgat gacaaaattg cttcttattg ccgataggct
    33421 gtctcttgaa caccatttac caagaggtcg tgagcctacc gtttaactct gagttacaag
    33481 gaacaatttc aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt gaccgaggag
    33541 ttggcaggag aacaccgttt attcagaaat aggagctacg acaaaagtct tgtcatagct
    33601 cctatttttt gtcattcatt tttcaagcgg tagaaatagc ctttttgagc taacagtttt
    33661 tcgtaagtac ctatttcaac gatttttcct tcatcgatca caacgatttt atctgcctct
    33721 ttgatcgttg aaagccgatg agccacaata aaagtagtgg gtgtttctgt caggtaattc
    33781 agggcttgct ggattttttt ctctgactgg ttatctaaag ctgaggttgc ttcatctagc
    33841 aaaatgattt ttggctgacg tatcaacgct cgtgcaatgg agatacgttg cttttggcca
    33901 ccagacagct tgttgccatg ttcgcctact aaggtatcca gaccatcagg aagctggtca
    33961 atcagtgaag aaagttgcgc agcttcgatg acttcatcta aacgttcttt tgatacgttt
    34021 ttcaagccat aagtgatgtt ctctttgatc gaagcggaga acaacaaagt ggtttgagga
    34081 acaacggcta aataatttcg cacacttcgc atatccagtt catccaaagg ctgatcatct
    34141 agaaaaattt ttcctgaagt cggctggata aatccaatca ataagttaag gagtgtagat
    34201 tttccagagc cagatggccc aacaaaagca attgtttctc cagctttcac ctctagagaa
    34261 aaatgatcga taatcggttt gagtgagtcc ggataaagaa aagaaacatc ctcaaaacga
    34321 aaatttccat ccaaatgttg gatttttttc ttaccgtgat aagtttctac atcgccagca
    34381 atcaacagtt cgccaataga agtgatagat tccatgcctt ttgaaatcgt aggaatcagc
    34441 gtgatcattc cagaaacgga attgacgatc gtcgtgaaat acgtctgata catcacgaca
    34501 tcgccgggtt ggatgcgccc ttgtaaagct aatatcccag tgaaaatcaa acaaatcagc
    34561 tgaaatatct gaaaactagc ccagctgact gatccgaaaa cagattgcag gatatctaag
    34621 tggaatcctt tttcatagat ttgagtaaaa tgcttattta agcgagttgt ttcgtgtttt
    34681 tctaacccat gagctcgagc aatcggtgct aattcgatca cttcgatcac ttttgcagaa
    34741 gtctcttcca tttcttgcct gaagtctgag ttggactgtc tgatcctttt acggaaaaag
    34801 agagtaacga atgcggcgag aggaacagtc aataagaaaa acatgaaaac aacaggactt
    34861 ttataaagcg tgatacctaa agctacgaat aaattgacag cgatatttaa tccgctgaca
    34921 aatacttgag aggacaacgt ttggatagct tctacatcac ggatgatctt tgactggatt
    34981 cgtccagatt gcaattcttt ttgataagga atagatagtt cttggatttt cttgataagg
    35041 gtcgctctta gtccagcttc tacttgacgg atcgaagtgc tatggtattt tacatgaaaa
    35101 taattcgtca atatattttg accaatcaga caagccatca aaaaagcgtt gaaataaatg
    35161 gcctgcaact gagaagaatc tttcgatgaa gctgcattga ttacattggc aatagcgatc
    35221 ggcattaacc aagatggaag atgtttgata ataaaaaaga gtcctgacat ggcaaaacgc
    35281 caataatttc cctgataaag atgcagcaat gtctgaaaag catttgcatg atatttttga
    35341 taacttttct gtattcgttc ctccataatc ttctccttaa caatcttggt aaataaactg
    35401 caagtgttct attgcttcag gaacataaat ctcaggagta gcttcaagag tggcgtacat
    35461 tagaggacgc tcatatttga tataatgcag taccgggcgg aaatctaaac agcccttgcc
    35521 aacaggtacc atgcggatct ttccattttc catgatatag tctttgagat gaagcgctgt
    35581 aatataacca tgtaaattgt ccaaggcacc tcggatcact ttttcctgat cagcatgatt
    35641 ttctttatgc atcaaattcg cacaatctaa aatgattttt aaatttggtg aagctacttc
    35701 atcgattaaa cgtttagcta actgatagtt atagattgga tgattgatcc ctgcttcaac
    35761 agcaacagtt acgccaaaat tttctgcatg agcgactaat tcaatgatag aatttctggc
    35821 gatctgataa gcttcttcag taaaattttc ttttgtgtaa ccgacagaga cactaccggt
    35881 ttccgtagca atcatcttgg cttgataggc agagcacaga gcaatttgct gtttgaaagt
    35941 tgccaatgct tgtgcacgaa cttcttgatt tttgctggat aggttgatat agcatcctaa
    36001 aatggaaagt tcgatattgt ttttttggaa gtattcacca aaataggcag cactcccaag
    36061 tgtcatgtca gttaatttaa gttgttcagg aaatgctttt tgtggtgcaa attggatatg
    36121 ggacaagtga agctgtgata ctttttgaac gatttcttca cgatttcgaa catctaaatc
    36181 gtgggcacgg atgcctaaat ttaatggaaa catctcaaac tcctttttgt aaataattag
    36241 caagaggcag acgggattct tttatcaatt caacaattat tcgggcaact ttatcagcgc
    36301 cttctggact aaagtgggta ttgtctttta gtccttctgg gtaattttta caagtatcag
    36361 gtataagatg caggtagaat ttttttgtct cttcttttga gaaggtctgg aacaattctt
    36421 gtgatcttga gaacacatct aaaagaggaa tatggtgttt ctcagcaaat gcacgcatcg
    36481 cttttggata gtctccaagt atatctggat tcaatgttcc gttttctaag taatctcgtc
    36541 gcgtcacaga tgtcagtaag accatttgga cgttcgcttt ttttgcgcat tcaaaaaata
    36601 tctgcagatt ttttaaatag ctaccgaagg gaagcgtacc tctttcttct tggattttct
    36661 ggtcgttatg tccaaattgt acaaataaga aatcattcgg ctgaaactgt gaagctagac
    36721 gggctaacct tccttcctgg ataaatgatt tggtgctgcg tccattcttt gcttggttat
    36781 tgatctggat ctcgggtgaa aaataagcag agattttttc tccccaacct gtctcgggac
    36841 gcttgttttc atctttttct gccgcagtag aatcaccggc aatatggata gtaggcatgt
    36901 tatctcttct ttcataaagg tttataggca atgaagcaga acagcaagag aagaagacgg
    36961 agcaaatttg gtccgtcttc ttctctaaaa cgtggataaa cggattccgt tatttcacta
    37021 tcctgttttt gattcgttac tgttactatt aatcaattag gcaaaaaagc caaagtaatt
    37081 tttagcattt gtataactaa tcgcttgaac tagacttcct aaaagttgtt catcttcagg
    37141 tgcttctcct cgcatgacca tttcgcctaa aaattcacaa agaacacgac ggaaatattc
    37201 atgtctagtg taggacagga aacttcttga atctgtcagc atgccgacaa aattccccaa
    37261 agcactacca tcagcaaatt gtgtcaattg atcgcgcata ccagctcgag tatcgttgta
    37321 ccaccatcca gaacctaact gcagttttcc attctgttct ttttggaaac agcccatcaa
    37381 agctaaaatc gcaggatagt cattcggatt caatgaataa aggatcgtct ttggcaactg
    37441 atccgtttct tctgcacggt tcaataattt ttgcagagga atagtcaaag aaagatcatt
    37501 cattccatca tagccggtat cgggccctaa tttttccaac atttgtgtat tacagttacg
    37561 ataagcatgg atgtgcagct gcatcgtcca attatgagaa tgatagaagt gggtcaaatg
    37621 tatcagtgtt tctgtacgat aagcatcgat ttcatctgaa gataatagtt tgttagctcg
    37681 tgctttttga aaaatctctt ctaatactgt ttcatccgct tctttatagc gtaagatgtc
    37741 taaagcgtgg tcggaaacac gacatccatg ttgatggaag tattctatcc gctgccctaa
    37801 gacttctatc agttcttgat aggtggtgat cgtttggtca gctgcttctt ccagtagtgt
    37861 gacccatgca ttaaatcctt ctttttcaat attgagtgct ttatctggac ggaatgaagg
    37921 tagaaccttc aaatctggtt cttcatgagc aagcaactga tgatagtgca agtcgtctgc
    37981 gggatcatct gttgtacaaa tcacttctac attcgatttt tttgcgattt cccgacggcg
    38041 aaaatcagga gctgccaaaa tttcatttgc ttgtgtccaa attttctctg cattttcttc
    38101 atttatcaac aagtcaatac cgaagaaacg cttcaattca agatgtgacc aactgtaaag
    38161 cggatttccg ataatttttg gtacggtttg acaccaagct aaaaattttt caaaatcaga
    38221 agcatttcct gtgatttttt cttcaggcac gccgcaagct cgcatcaagc gccatttgta
    38281 atgatcgcct cctaaccaag cttctgtcag attgctgaaa ttgttgtttt cgtaaatttc
    38341 ttttggatct aagtgacagt gataatcaat aatgggcatg ttttttgcgt aggaatggta
    38401 aagctttttc gcccaatcgt cttttaataa aaaatcatca gataaaaaca taaatcttcc
    38461 ttctttcttt ttatactgtt ttatgaaaat caaagtgtta ctccgttttt aaaaatggca
    38521 atttctcgaa cgtcattttg ttcatttttc gtttcttctc cactagctac agcgatgatt
    38581 ttttgaacaa acgattctaa gattacttcc attggttgtt ctaataagac gcctgcatta
    38641 aaatccatcc aatgaccttt gcgttcaaaa agagttgtat tcgtagatac cttgaccgtt
    38701 gggacatatg agccaaacgg agtccctctt cctgtggtaa acaagaccaa ctggcaatcg
    38761 gatgaagcaa gagcagaggc agctacaaga tcattcccgg gagcttgcaa taaactaagc
    38821 cctggttttc tgattttttc gccatattgt agcacatcaa cgacaggaga gcttcctgat
    38881 ttttgcgtgc agcctaaaga cttatcttct aatgtagtga tcccacctgc tttattacca
    38941 ggagatgggt tttcgtaaac tggttccccg taagacaaga aatactgttt gaagtcattg
    39001 atcaagtcaa cgattgagtc aaaaacttct tcattttccg ctcgtgccat caatacttgt
    39061 tctgcgccaa acatttctgg cacttctgtt aagatcgtgc tgcctccttg agcaatcaag
    39121 tagtcagaaa atgcaccaag taaaggatta gcagttattc ccgatagacc atcagaaccg
    39181 ccgcatttca aaccgacatt caacttagag agcgggactg atatgcgctg gtcattttcc
    39241 gccgctgtta aaagctcttc taatagagcc acaccttgtt cgatctcatc gtaaacatct
    39301 tgagccacta gaaatttcac tcgttcttca tcatattcac ctaaaatttt tttgaattca
    39361 ggaactgtat tgttctcaca acctaatcca aatactaaaa ctcctccggc attgggatga
    39421 tttactgcgt ccgctaaaat ttctcgagta ttttgatgat cttttccaag ctgcgaacag
    39481 ccataaggat gctttaaaat cgtaatatgg tcaaactgtc ccagatctgg atgtttttcc
    39541 ttgaactgtt tgacgatcag ttcagcaatg ccattcacac acccaacggt aggtactaaa
    39601 aacaaatcat tacgaatccc tacttttcca ttttttcgca agtaaccttg aaatgttcgg
    39661 ttctcaaaaa ggtaatgact tggatggaca tctttttcat atcgataatc caattcacca
    39721 gaaagattgg tttttacatt atgagtgtgc agccattgac cggcagttac gtctgtcgtc
    39781 acatgaccga ttggatagcc atatttgatc acgttgtctc cagctttcaa atcacccaat
    39841 gcaatcttat gaccttgcgg gatatcttca agagtggtga tcgtttgctg atcaatcgtc
    39901 agttgtgttt gttttggaat aggggttaat gcgacgacca cactgtctcg tcgatttaat
    39961 ttcattgttg gttgcagggt ttcaagcatg gaccatcgtt cctttcattt cttgtaatac
    40021 tgaccggaca cctttttgat cgatctttgc agctatttct tgaactgtat ctgtcacatt
    40081 cggtatttga gacaaatctt ctccccaaag cgcagcgttt tgcaataatc gaggaatagc
    40141 tgttccatta tcagaccaag catcagtaaa ttcgcttatc gtcttttcat catcatgtgg
    40201 ctttacttga tcgcctcgat acataagaag tagcgcagct agtgaaacag tgattaacgg
    40261 tggtacctga ttttgttctt cgatatactt cttgaacact ggaagcagac gggtgctgaa
    40321 ctttgaaaca ctatttaatg aaatcgcttc taatttgtga tgggcaaaag gattcaagaa
    40381 acgttctttg atttggtcag cataaatggc taattcatct ttaggtaaag aaagcatagg
    40441 aatcaattct agattgaata actgatcaac atatgcggag aataaaggat ctttcattac
    40501 ttcttctaca gtttccaatt ccgccaacaa agcaagaggg accatcgctg tatgcggtcc
    40561 attaagcaaa tgcaccttac gttcacgata aggagtcata tcatctgtga tgatgacatt
    40621 caagccagcc tgtttgaatg gtagttgatt ctccaattct ttaggccctt cgatcaccca
    40681 aagcatgaat ggttctgctt tgaccatcaa ttgatcttca tagttatgcc gttgattgaa
    40741 aacacctgca tcttcgcgag gataacctgg gacgatgcga tcaactaaac tacaacagaa
    40801 aacattttct ttttctaacc actcaacaaa agcaggttct aaatcccatt tttctgcgta
    40861 gcgtaaaatg atttctttta gacgttcgcc gtttctgtca atcagttcgc aaggaatgat
    40921 ggtaaagcca gctttgttta atttgaaacg tcggtataaa aaggctgtca gtttgcccgg
    40981 gaagctctgt gggggagtat ctgttaaatt atcagtctcc acgaattgga ttcctgcctc
    41041 tgttgtattg gagacgatat aggccaagtc gtcattttct gctagagata gataagcatc
    41101 ccattcatca taaggattga tcatttgttc tactacggtg ataatctcgg aagtatcaac
    41161 gatttcgcca ttgctgagtc cttgtaaaat gaccgtatat aaattatctt gctggttcat
    41221 catatttcca agaccttgtt ctaaaggttg aatgattgca acattacctt taaacaattg
    41281 ttggttattc agctgctgaa tctgccaatc aataaatcca cgcataaaat tgccttcacc
    41341 aaactgaaga acttttacag gtgcttcgtt ttttttcact aattcttttg tcagtttttt
    41401 catataaact tcctttctgt gttatgcaca cgttaacatt tttatataaa attacttgta
    41461 gtttacaagt aattgatcga ctcacgaagg atcaacaagg tggagagctc aattttagat
    41521 tgttcgatcg tcctgccttc gatcaaatct agtaaaagct ttgttccttc ttgacacatt
    41581 tctttcaaag gcttacgtac ggtggtaagc ggcggattga gataggagac gactggagaa
    41641 tcatcgaacc cgatcagaga aatgttctgg ggaacacgga ggccagctaa atgtacagct
    41701 ctcaatgcac caatcgctat gtcgtcgttg ccgcaaaaaa ccagagtagg caggtcagaa
    41761 agtgataata ggtattccat gttttggagg ccgctttcta agctgtaatc tcctgttaaa
    41821 aaataggctg gattgacagg gatgctcgct ttttttagac ttttcaatac ccctttttcc
    41881 cgttcttttg tcgaacgaaa atttttcttt ccgccaatat aagcgatctt cgtatgtccg
    41941 catccaatac cgtaatctac tgcctgcata acaccggccg catcatcggc agtgacgttg
    42001 ataaccgctg gatcattgat gtgacgattg acaacaacga aaggaaattc tttttctttg
    42061 agatagtcga tgaaaggctg atcatcatca ctttgactca taacgatcag tccatcgtag
    42121 cgttgcaggt tgatttctgt taaatcaaca acatcgatac tttcaacaga taaactgtaa
    42181 tttgtgtcta atactgaatg gatgcctgca atggaatctg ctaggaaatt tgatgaagtt
    42241 cctttatgca aacttgagaa aaatagtccg attagatatt ttttttgatt cactaaacct
    42301 tttgcactat agtttggcga ataattcatt tccttagcta gttgtcgtat tttttgtttt
    42361 gtttcatttt ttattaaact gctgttattc aaagcacgag aaactgtcgt atgagaaacg
    42421 ccagctgctt tagcgatatc tttgatcgtt accattccat cattttcctt actttttcac
    42481 tttttgttaa cgcattcatc atattacgat aactacgata agtcaataga tctttgatat
    42541 gaaaagattg cacacgttaa aaattacttg gatttttatt tttaattatg ctattcttga
    42601 aagcggggaa tgctcattta attagatgag tatcaatttt acagaaaaat gaaagccttt
    42661 tattttaaaa ggaggagagg aagaaaatga agaaaagtac agtattttat aaaatatttt
    42721 taccgatgtt tttattagga gccgtacttg tcataggatt tagtttattc atttaccaga
    42781 atacctatga atcgatcgaa tcgagttatt taacagacaa aaagaatttg ctcaggcaag
    42841 ttaaaactaa cgttgaatgg aaaattagaa cgattgaata ttcgttttct acgtatggtt
    42901 cgaccaaaaa tttctcggac atattcaagc atccgctgac atatacagat tactccactt
    42961 attctgaaat caggaaagaa ctaaatttta tagaaacgat tgttatggat gataataatt
    43021 atgatttagt cagtttggct ggtaaatggg gagtaatcaa tggatcgttg agtcaactga
    43081 ccgaagagga agttgaaaag tatcaacaga agtacataaa taacaaaaat aatttatttt
    43141 ggcaaaagaa aaacaaagga atcgaaatgg tgatttcttt gccaatgttt gaaaaagaaa
    43201 aatatgcatt gggaattgct acgatagaga aacatacaat agaacaagtt gttggtgaaa
    43261 gtggagaaga catactttct attgaaaacg aagttggcaa cctttttact actcagacag
    43321 taaaaaataa agagcaacca actgtattaa cggacgtgga tctagattac aatcagctga
    43381 aagtgataag agaaaaagat aaaacgtata tagtactgaa atcagattat aataattgga
    43441 tttacagatt agaagttgac tctagtgcaa ttggcgcaat gatccgcaat ctgagaatcg
    43501 ggctagttgc agtgtcttta acactggtag gactcattgg tcttctttcc tacttatttt
    43561 cggacagatt tgcccgtccg atttctcaaa tacaggaacg tctgaatctt aaatcaaaag
    43621 gattcagtgg gaaagaacta gatttggtcg ctcaatcagt cagtcggatc ataggagaaa
    43681 aagaagcgtt aagtgcacac ttagttaccc aaaagccaca gctggaaacg ctatttgtac
    43741 ttagtttatt tagaaatcga gtagaaaggc gagaactaga tcagcggttg cagcaatttg
    43801 gttatagttc tcaaaatgac tgctattata cagctttggt tcaaatcgat ctgttagaag
    43861 atagtcacgg tggggagcgt gatttgctac ttttagcgat caacaacatg atcacggaaa
    43921 ttgtgccaaa ggaaaatcga atgctgccga tcgttttgaa tgaagaaatg caagccacta
    43981 tttatcgaat ggataaagat gatgcagaag caagcaaaaa agtcatggag tattgccgac
    44041 tcattcaaaa acgtgtaaaa gaatacttga atttaactat cagtataggc atcagtaacc
    44101 ggttcgaaac gctgaatgaa agcaagcaat cggttgatcg ggcaaaagaa gccctgtatt
    44161 atcgtgtaaa tactggtcca agttcaatta ttttttatca ggaaatcgtt cctgctgctc
    44221 atgaaaaaac acttattaga tatccgatag aagaacaaaa tcgattgttt gaagccattc
    44281 gttctggaaa tcaggaagtg aagaatttag tacatgaatt gattgatgca ttgtttgcac
    44341 aaaataaaaa tcctttgagt cgggaagtag tttcggtccg cttgatcaat gaactcgttc
    44401 aattaggaca gttattggga gtagattcaa aaaactttga taacatgaaa caaatttatg
    44461 taaaagcact gaacaattat catcccaaag aactagaaaa acttgtttta gaacaattag
    44521 ttctgccaat cacagaatgc tctcaagcaa tcactgatca tgaatttaaa aatttgtcgg
    44581 agaatattat gcatatcgtc tacacagaat atgatcgtga tctgtcttta gatgtgatcg
    44641 ctgataggtt gcattataac cctaactatt taagtaatgt atttaaaaag gaaacaggag
    44701 aaaaatttgg tgattttgtt caaaatcatc gtttagaggt agcaaaaaaa tggttgaaag
    44761 agactaacct ctccgtaaaa gagattgcag aaagattgca atatcgaaat ccgcaaaatt
    44821 ttattcgttt ctttaaaaag aaggaatcga ttacgccggg agaatatcga aaaaaatatt
    44881 tgtgaatcaa gaggctgttt ttcttgtgga ctgattcaag caataaaaga agttagaata
    44941 accctagtat ttgtatttat ggatagttaa atttcaaggc taagcacgca aaaagaactg
    45001 tgacaaaagt cttgtcacag ttctttttta cgaataaatg gcgttcaaga gacaggctat
    45061 cgacaataag gcataatttt gtgaaatatg ggaagctatt tttcaaaatc gcttcttatt
    45121 gctcctgtgg gttactcgtc gcaatgtttg tgagagtttt tatcatcgct caaggctgaa
    45181 cgtctctttc acaacctctg atacacggtg ttccaggagt agattcttcg aaaaataagc
    45241 cgaaatttca caaaaattta agaagcaatt ttcgtaaatg ccttcttatt tcttgaagct
    45301 gaccacttct gacacaacta ccattttgcg ttcagtacat aatcaccctt tgacagaacc
    45361 tacgagtgct cctttgacaa aatatttctg aacaaaagga taaacaatca gcataggcac
    45421 tgttgcaata acgataacag ccattttaac tgtttgtgct ggtggaatca catcaacggc
    45481 actactttct gcttgcatcc cacttgatac aatgacgatt tggcgtaaaa gaatctgtat
    45541 cggccattta gatgaatcgt tgatatacaa aatagcagtc atataagtgt tccagtaagt
    45601 aacagcataa aaaagcgaga tagtagcaat agacggtaac gctaatggca gcataatttt
    45661 gaaaaataca ccaaaatctg tgcaaccatc catttttgct gattcttcca aactatccgg
    45721 caaggcttgg aaaaagtttc tcataatgat catattcatt gcattaacag ccacaggcag
    45781 gatcatggcc cagtaagaat tgaccagccc tagagaacga acaactaaga acgttggaat
    45841 catccccccg ctgaacaaca tagaaaatac aacaagaaag ttgacgaaac ctcgaccaaa
    45901 caaatacttt ctggaaagac cgtaggccat cagtgaagtt gcacacatac tgacgatcgt
    45961 tccgacacct gtaacaccaa tcgaaacagc aagtgctcta aaaatggtcg gcgtagaaag
    46021 aatgtagcga tacgcgtcta aagaaaaggt agtaggaaaa aggataaact ttttcgcgac
    46081 gacttcttgt gtagaagcaa aagatgtagc gatgatattt aaaaaaggaa cgatacagat
    46141 caaagctagc aaaattagaa acgtattgtt gaaaagtgta aaacttcttt ctcctataga
    46201 gactttcttt ttcatataga taggctcctc ctcaaaataa attaaatgtt tctgtttcta
    46261 tatgttcaat ttatcatcgg tgaaagatta cgtatataat catttcctat gctaagtgtc
    46321 agatagaaaa aaatagaagg tttgctatgc gtagtttatt tgtgaaaaaa tacactttat
    46381 cgttttgtat agcacaaaaa tcattgctat gtaagcgttt ttacttacgg taaaaatgat
    46441 tatatacgta attcagatag ttatctatca tgaagtcata agcagcaaac attcacaaaa
    46501 ctaaggaggg gcttatggaa agtgtaaaga aacaagaacc atttcaactg acgctatcga
    46561 aaaaagaaga aaagctcaga agacgaaaaa aaataattgc aggaatcaaa acgaataaat
    46621 ttttatatgt gatgattctg ccgggagtcc tttatttctt gatttttagg tatcttccta
    46681 tgggggactc gttattgctt ttcaagatta tcaaccattt ttaggaataa tgggaagtcc
    46741 ttgggtcggt ttcaaacatt tcattcgttt gtttacagaa ccgacattct ttacattatt
    46801 aagaaatacg ttgatcttgt tcggattaaa tatcttcgtg ttttttccga tgccaatcat
    46861 tttgtcttta ctattaaatg aagtaacgaa caaacgattg aaaaacggga ttcaaacgat
    46921 catttacatc cctcacttca tgtcttgggt aatcattgtt tcgattactt atgtattttt
    46981 gaatgttgac ggaggggtat tgaatgaatt ccttgcaaca atgggatttg aaaaaatcag
    47041 ttttctaaca tcccctgaat ggacacgcac agtatatatc ggacaagtga tctggaaaga
    47101 gttgggctgg tctacgatca tctacctttc agcgataact gcagtcgata cacagttgta
    47161 tgaagctgca gaaatggatg gagcaggacg cttacgcaaa acatggcatg ttacattgcc
    47221 ggcaattcgt ccagtaatca ttaccttgct gattctaaaa atcggtcaga cattagactt
    47281 agggttcgag catatgtact tgttattgaa ttcattgaat cgaagcgttg cagaaatttt
    47341 cgacacctat atttatacag ctggattgaa gaatggacag ttaagtttca gtacagcaat
    47401 gggattgttt aaaggggtcg taggattgtt tatggtaatt ttagctaata agattgccaa
    47461 aaagctagga gaagacggcg tttactaaga tagaaagaga gggaaagata tgctgcagtc
    47521 attagataaa ataaacggaa ggctaaatcg tattttacag ttggtttatt tgaattttct
    47581 gtggggcggt ttagtcatcg caggtttggg agtttttact ttcggtcctg catcgtatgc
    47641 gatggttgca gtgattagaa aatggctgcg ctcaaaagaa acgtttcctt tgacgaaaac
    47701 gtacttcaca tattttaaag aaaactatcg tgaaaccttg ctgatgagct ggatatatgg
    47761 cgccatcggg attgttttac ttgtggatct ttctttcata cacaattggt atcttaggat
    47821 cgctttgtat attgctgcgt tcatatatgt gctctcagca ctctatattt tcccaatcat
    47881 ggctcattat gattggaaag gatatttttt caaaatgaaa atggcatttt tattcggatt
    47941 ctcacatctt cattactcat tagtgttgat attaagtatg gtagggattt atctgatgat
    48001 catccaaaca atgccaggtg ttttaacctt tatcggtggc agtctcttct tttccgcagt
    48061 cacgtggaca gcaaattcta tattttgcca tttggaatgg aagaactcat atacagaaaa
    48121 aacagaagaa aacaaaaata ttattaaaac agtgaaggag aaaatttatg aaaaaagcaa
    48181 cgctcgtaaa attaggtctt agttttgcag gaattctagc acttgccgca tgcggcagca
    48241 gttcaagtaa cagttcttca gagacagaag atggaaaagc taaaatcact tggttaaata
    48301 ttttgcatca tgcttcaccg cctacagaca cgattgtgaa cttgatcgaa gaaaaaaccg
    48361 atacggaact gacattcaac tggattcctg atgcttcaaa agaagaaaga ttaacaactg
    48421 cattggcttc tgatgaacta ggagatatcg ttacacttca aattgcgatg ttgaaaaact
    48481 cttctgtccg caatgcgtta aaatcaggcg tattctggga tgtatcagaa tatttaaaag
    48541 actataaaaa tttaagtcaa atttctgaag atagaatcga agctgcgagt atcgacggtg
    48601 ccttgtacgg cgtaccgata caaaaagact acgctcgtgc tggcttagtc attcgaaaag
    48661 actggttgga taatctagga ctagatgttc caactaccat ggatgagctt tatgaagtag
    48721 ctaaacaatt tacagaaaac gatcctgatg gaaatggtgt ggacgacaca gttggttttg
    48781 gcgatcgtgc gcctagtgat attcgttata ccagctttaa acttttcact tcttattttg
    48841 gagcaccaaa tggctgggct gtagacgata atggcaaatt cactccagaa tttgatacag
    48901 aagagtatat gaaagctttg aatttttcaa gagatctatt tgagaatggc tatttggcac
    48961 aagactttgc agttacccaa aagactgatc agcaagaaca atttgcacaa ggaagaactg
    49021 gaatctacac tggaatggtg gatatcaaga atctgaagac gatgggtcaa ggtattcaag
    49081 atgatatgga attagtgcct gtaaacaaga tctcagatgg tgaaggcggc ggttatcatg
    49141 tttggtcaga aggaaatggt attggcggct tgctagcatt tcccaaatct caagtaaaag
    49201 acgaagcaca attgaagaaa ttacttcaat ttgttgatga tcttttagaa gaagatgtgt
    49261 ataaattgat gactggcggt atcgaaggaa cgcattatga attgaatgat gatggttcta
    49321 tcgaatttat cgatatggat ctttggcaac aagaggtgca accgttatca agctctcgtc
    49381 ctagtgaagt gacgttctct ttcaaagata cagaccctga aaaagaattc tctaatcaat
    49441 tgatcaaaga aaatgaagaa tttgctgtaa tggatccttc tgtgccattg gattcaccga
    49501 cgaacaatga agtaggatca gaaattgaaa aaatcgtcat tgatgctacg gtgcaataca
    49561 tcatgggaca gcttgacgaa gatggattca aaaaagctgt agaagactgg aaagcacaag
    49621 gaggcgatcg aatcacacaa gagtacgagg aagcatacaa agcagcggca cagtaatata
    49681 gacgattggg acaaatactt cttggtattt gtcccaaatc ctattttaat ttttaaagga
    49741 gagcaaataa tggaaaactg gtcaaagaaa ttagcaaaaa gtatcatgga aagaacacct
    49801 cgcttatatg aagaaaagtg gtataaagga aaatggtctt atgactatgg cgtcgtactt
    49861 aaagggttcc aattactttg ggaacgaacc caagaaaaaa tttattttga tttcataaaa
    49921 gacaatatcg actatttcgt gcaagaagat ggaacgatcc gtggctacag cgttgaagaa
    49981 tacaatatcg accatgtaaa tacaggaaag cttttctttt tattgtacaa agaaacaggc
    50041 gaagaaaaat ataaaaaagc agcagaactc ctttcccagc agttggcaaa ccatccacgc
    50101 acatccgaag gagctttttg gcataaagaa atttatcctt atcaaatttg gttggatggc
    50161 ttatatatgg gttctccttt ttatgctgaa tttatccaga attttagtga agaaaaagat
    50221 taccaagatg ttttgagaca gtttgaaatc agttataaac atctattcga tccaaaaaca
    50281 aatttgctga ttcatgcatg ggatgaaaag aaggtccaac catgggctga tccaaaaaca
    50341 ggtctatccc atcatttttg gagccgatcg attggctggt atttgatggc tgcagcagat
    50401 acctatgaat tacttcatga agaaacagat tgttctttgc tgaaagaaat tctagaaaag
    50461 acactggctg cattacttcc atatcaagaa gaaaacagcg ggacatggta tcaagtaaca
    50521 acagaaacaa aacggaaagg aaattatctg gaagcttccg gcagtagtat gtttgtttac
    50581 gcaatggcta aaggaatacg tttgggactg ctgaacaaag aagaatggct tccacaagtg
    50641 aagaaatcac atcaaggatt acttgatgaa tttgttctgg aaacaaaaga aggctggctg
    50701 aacttgaata aaaactgtca agtagcaggg ttaggcggag cagatcagcg agatggttca
    50761 tatgcttact acatcagtga accaattatt tgtaatgatc aaaaaggcgt tggtgcattc
    50821 ttgcaagcat tgattgaagt ggaggcatta taaatatgta tgatattttg acatttggcg
    50881 catcaattga tgaattgaat actgaagcaa tacaacaagc cattgatgct gcagcaagtg
    50941 acggaggcgg gaccgtagtt gttccagcag gtgaatttct aacaggagct ttatttttaa
    51001 aaagcaatgt cgaacttcat ttaagcgcag gagcggttct gaaattctct gacgatccaa
    51061 aagattatcc tgttgttcat tcacgttggg aaggagtcca tcgaaaagtt tatgcttcct
    51121 gtatctatgc tcaaaatgta gagaacatct ctgtcactgg tttcggtaca ttagatggaa
    51181 atggaaaaaa atggtggcat acttttagaa atgaaccaga taatctcgct tatcctcgtc
    51241 ctaaattgat gagtttccat aactgtcatc gaattactgt gaaagatatt aaattgatcc
    51301 agtcaccaag ttggacgatc aacccgattc tttgcagtaa tgcaacgttt gataacttga
    51361 cgatcttgaa cccagcggat tcaccaaata cagacggaat tgatccagaa tcatgcaaaa
    51421 atgtgcgtat tagtaattgc catattgacg tgggagatga ctgtattgca atcaaggcag
    51481 ggactgaaga tacgtacgaa aggatcgctt gcgaaaatat cacgatcaca aattgcacga
    51541 tggtccatgg acatggcggt gtcgtattag gaagtgaaat gagtggtagt attcgtaata
    51601 ttaccatttc aaattgtatc ttccaagaaa ctgatcgagg aatacgattg aaatctagac
    51661 gaggtcgagg cgggatcgtt gaagatattc gagtatccaa tatagtcatg gataatgtga
    51721 tgtgtccatt tatactgaat ctttattatt tctgtggtcc gcgagggaaa gaaccgtatg
    51781 tatgggaaaa aaaggcttat ccgatagatg aacggacacc agcattcaga aggatccatt
    51841 tttcgaatat cacggcaagg aatgtccatg cttctgcagg ttttatttat ggattagcgg
    51901 aacaattcat acaagaaatc acatttgaca atatcgatgt ctcgatggcc caagatgcag
    51961 ttcccggtgt tcctgctatg atgacaggaa tagaggaaat gagcagacaa ggattttata
    52021 ttggctttgc gggtaaggtt tcctttaacc agatgtcgat tgaaaatcac gagggaccag
    52081 catttctatt agaaaatact aaagaaatca gtattgagaa attccaatca aatcaatcag
    52141 aaaatatgga tgaactggtt gttcgtaaaa actttgctta tgcagttgaa tgatttttta
    52201 ggtgctccat ctagcagaca tacccctagt ggaaaacgat tataccgaga aaaacaaaac
    52261 ggttttatgc ttgaatacta tcaattgtac ttaaatgaat tggaaacagt accagccctc
    52321 tatgcttatc cagaaaaaga aggaccgttt ccgccggtcc tttacctgca ttctcacggc
    52381 ggagattttt cagtcgggaa aagtgaattg atccatggtg ctccttactt agcttctcca
    52441 tcttttgcag aagtactgac aggaatggga tatgctgttt ggtcgattga tgcttgggga
    52501 tttgaagaac gcggaggtat ttctgaaagc gagttgttca aacaattttt attaacagga
    52561 aaaacgttat ggggaatgcg tatttatgat gtgatcagct tgatcgatta tttggagaca
    52621 agggaagaca cagatactca acggctcgca acgattggtc tttctatggg aggcttatta
    52681 agctggtggg tgacagcatt ggattctcgt gtcaaagttt gtattgatct agcggcacag
    52741 gtggacatcg aaatactttt gctacatcgc cgcctagatc atcatgggtt ttattactat
    52801 gtgccaaatc tattgacagc ttatacaacg ttagcaatcc aagaaaaaat cgctccgcgc
    52861 cctcgtctta gcttagttgg aaaaaacgat acgatgtgct tggacgaagg cgtactgaag
    52921 ctgagaaaag ggctggacaa aaagtatcaa tcaatgggta gtccagaaaa cttttcttct
    52981 ttttctttaa caggcggtca tatggaaaca caagaaatga gaaatatttg gaaacaattc
    53041 ataagggagc atttatgagg atgaaagaca aactgataat tggaaagaat gctatagata
    53101 ccccgacgga ttttgaaact atccaagaag gaatcgatta tttggcaggc ctgccggata
    53161 gccatccaaa ggtcttgata gtaaaagaag gaagatatga tgaatatata gaaagcagat
    53221 tatccaattt taaacttgtg ggagcaggaa cagtagttat ctccggtaat cgttttgcaa
    53281 aacaggagta tcttgacggt agccagttag ggactttccg aagtgctact tttttcttag
    53341 aaggatcgaa tgtcgtatta gaaaatcttc acatcgaaaa tacagcaggt ccaggagaaa
    53401 aggtaggaca ggctgttgct ttattcaatt caggagatcg cgtatcttta aaaaactgtc
    53461 gactatctgg ttatcaggat accttatgca cgggtccgtt gcctgatttg caaaaagatg
    53521 gtactccttt ttgtacaccg gcttctgcca atcctgagta ttgtcgtcag acctatgaat
    53581 attgctggat tgaaggaacg gtggatttca tatttggtgg ggcagatgcc gtttttcatc
    53641 agtgtcatat ccatagctta gccagtaaaa atcctggata catcacagca gcatctacgc
    53701 cgaaaaatca aaaaacaggg tatcgttttg aaagctgtac cttgactgca gaacctgata
    53761 ctagaaacgt ctatttagga agaccgtggc gtccgtatgc aaatgtctta tttgaagcgt
    53821 gtaagatagg tgggcatgtt cacccaactg gttgggataa ttgggatgac aagaaaaatg
    53881 aacagactgt caaattctct gaacgtaata acaattatga aacatcgatc aaaagacctg
    53941 aatggataca tttagaaaag cagggggttc agaaatgaaa agaaaatggg aactattgct
    54001 aggaatggtc ggtggcagtc tatctttgat tttctttggc gggttagcag ttactttaag
    54061 taatatgtct gcgtcagagt ttaaaaaaag ttatcagtcg cttgctgtag atcattcaac
    54121 tttgtcttta gaaaatacat ttggattatt acaggacatg actggtctgt ttgccgttgt
    54181 gttgtttatt tcacttgctt ttttagcagt agcattgttt ttgactgcta aaggaaaata
    54241 cttaacaact gccactggtt tgtattttat aacaggattc atattgttga ttggcactca
    54301 gtttattgcg tttccttttg cattttttta tttcgcagct ggggcattca gtttatatcg
    54361 agtaagaatg agaaaaggag cctgaatcat gcagactatc aaagtgaaaa atcctatttt
    54421 aagaggattc aatccagatc ctaatatatt aagagtgaaa gagcggtatt acattattgt
    54481 ctcctctttt gaatggcttc caggaatccg agtatatgag tcgaaaaatt tagtcaactg
    54541 gtcacataca acggatattt tgaccactca agtatctttg caaggaaatc ctacaaatgg
    54601 aagtatatgg gcgccacaaa tcagttatgc tgatggattg ttttatcttg tttacacaga
    54661 tgtcaaaagt gcaggccgtc cgtttaaaga tgtccacaat tatttgatca ctgcatctga
    54721 gatcacaggt ccttggtccg aaccaattta tttaaatagt aacggctttg atccttcttt
    54781 gttccatgat cttgaaacgg gaaaaaaatg gcttgtcaat gaactatggg attatcgtct
    54841 ggacacaccg aataaatcca gcggtatcgt attacaagaa tatgatgcag agaagaagaa
    54901 gcttgatggt cccatctata aaatatttgc aggtacagaa ttagcgaaaa cggaggcacc
    54961 acatttgtat tttcgtaacg ggtattatta tttgatgacg gcagaaggcg gtacaggaag
    55021 aggacattca gttacagttt gccgagcaaa gaatattact ggtccttacg agcttgatcc
    55081 tggttttcct atgctgacag ctagtgacaa acctgcttct acattgcaat gtacaggaca
    55141 tgggagctta gtacaaaccc cgtcaggaag atggtacatg acatatcttt gtaccagacc
    55201 attaaacggt gcggctattt tgggaagaga aaccgctatt caagaagtgt attggacgtc
    55261 cgacgattgg ttaagactta aagatagcca gacaactccg atggaagaaa taatgattga
    55321 gacaaatgaa gaggtacaac aagaaaaaga tcatcagttt atggatacat ttgaaggaga
    55381 gctaaagaag aactggaatg ccagacgcat tttgccaaat gctgattggt gtgatacaaa
    55441 atgtagacca ggttttttac gtctgatttc cggtgaatct cttcaatcta ccttcgaaca
    55501 tcatttgtta gctattcggc aaacggattt tacttttgat gcggagacag catttgaata
    55561 catgcctaat aattttaatc agatggctgg actggtactg tacttaaatg aatccaatta
    55621 tatttattgt tatgttactt ggaatgagga caagggaaaa tgcctacgta tgatagaatc
    55681 agaaaacgga gtagcacaaa tagaagattg gacgattcca ctcacagaaa agaagacttt
    55741 tttgaaagtc actgtaaaga aagagacagc acagtttcat ttgaaagacg tatcaactga
    55801 agaatggaag aaagtaggtc cgcaaaaaaa catgtgtata ctctcaggag ggtttactgg
    55861 aaattttatc gggatagcag tgcatgatct aaataaaaaa agaggaagtt atgctgattt
    55921 tgagtttttc aagtatgatg gaaaagatca tctgtaattt aattctttaa taaatgaggt
    55981 tcactaactt attttcacta gttagtgaa
//
LOCUS       DACCXM010000005        55265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCXM010000005 DACCXM010000000
VERSION     DACCXM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.005"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            4..861
                     /locus_tag="IVY61_000234"
     CDS             4..861
                     /locus_tag="IVY61_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP7969375.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            976..1896
                     /gene="pstC"
                     /locus_tag="IVY61_000235"
     CDS             976..1896
                     /gene="pstC"
                     /locus_tag="IVY61_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP7969376.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1896..2780
                     /gene="pstA"
                     /locus_tag="IVY61_000236"
     CDS             1896..2780
                     /gene="pstA"
                     /locus_tag="IVY61_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP7969377.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2795..3601
                     /gene="pstB"
                     /locus_tag="IVY61_000237"
     CDS             2795..3601
                     /gene="pstB"
                     /locus_tag="IVY61_000237"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7969378.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3617..4375
                     /locus_tag="IVY61_000238"
     CDS             3617..4375
                     /locus_tag="IVY61_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7969379.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4388..5065
                     /gene="phoU"
                     /locus_tag="IVY61_000239"
     CDS             4388..5065
                     /gene="phoU"
                     /locus_tag="IVY61_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP7969380.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5303..5602
                     /locus_tag="IVY61_000240"
     CDS             5303..5602
                     /locus_tag="IVY61_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969381.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5628..6335
                     /locus_tag="IVY61_000241"
     CDS             5628..6335
                     /locus_tag="IVY61_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7969382.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6332..7099
                     /locus_tag="IVY61_000242"
     CDS             6332..7099
                     /locus_tag="IVY61_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7969383.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7199..8767
                     /gene="liaX"
                     /locus_tag="IVY61_000243"
     CDS             7199..8767
                     /gene="liaX"
                     /locus_tag="IVY61_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP7969384.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9067..9384
                     /locus_tag="IVY61_000244"
     CDS             9067..9384
                     /locus_tag="IVY61_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP7969385.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9384..9734
                     /locus_tag="IVY61_000245"
     CDS             9384..9734
                     /locus_tag="IVY61_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP7969386.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9901..10839
                     /locus_tag="IVY61_000246"
     CDS             9901..10839
                     /locus_tag="IVY61_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP7969387.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10854..11684
                     /locus_tag="IVY61_000247"
     CDS             10854..11684
                     /locus_tag="IVY61_000247"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP7969388.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11728..12753
                     /locus_tag="IVY61_000248"
     CDS             11728..12753
                     /locus_tag="IVY61_000248"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7969389.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12768..13706
                     /gene="galU"
                     /locus_tag="IVY61_000249"
     CDS             12768..13706
                     /gene="galU"
                     /locus_tag="IVY61_000249"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7969390.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13831..14271
                     /locus_tag="IVY61_000250"
     CDS             13831..14271
                     /locus_tag="IVY61_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP7969391.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14274..14876
                     /locus_tag="IVY61_000251"
     CDS             14274..14876
                     /locus_tag="IVY61_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7969392.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14944..16050)
                     /locus_tag="IVY61_000252"
     CDS             complement(14944..16050)
                     /locus_tag="IVY61_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7969393.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16260..17261
                     /gene="ccpA"
                     /locus_tag="IVY61_000253"
     CDS             16260..17261
                     /gene="ccpA"
                     /locus_tag="IVY61_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP7969394.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17564..19909)
                     /locus_tag="IVY61_000254"
     CDS             complement(17564..19909)
                     /locus_tag="IVY61_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7969395.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20188..21090
                     /locus_tag="IVY61_000255"
     CDS             20188..21090
                     /locus_tag="IVY61_000255"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP7969396.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21303..23078
                     /locus_tag="IVY61_000256"
     CDS             21303..23078
                     /locus_tag="IVY61_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP7969397.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23335..24552
                     /locus_tag="IVY61_000257"
     CDS             23335..24552
                     /locus_tag="IVY61_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7969398.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKEALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(24999..26585)
                     /locus_tag="IVY61_000258"
     CDS             complement(24999..26585)
                     /locus_tag="IVY61_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP7969399.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            26836..27060
                     /locus_tag="IVY61_000259"
     CDS             26836..27060
                     /locus_tag="IVY61_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP7969400.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            27202..28953
                     /locus_tag="IVY61_000260"
     CDS             27202..28953
                     /locus_tag="IVY61_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7969401.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            28953..30737
                     /locus_tag="IVY61_000261"
     CDS             28953..30737
                     /locus_tag="IVY61_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969402.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            31195..32046
                     /locus_tag="IVY61_000262"
     CDS             31195..32046
                     /locus_tag="IVY61_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7969403.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            32143..34029
                     /locus_tag="IVY61_000263"
     CDS             32143..34029
                     /locus_tag="IVY61_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7969404.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(34070..34972)
                     /locus_tag="IVY61_000264"
     CDS             complement(34070..34972)
                     /locus_tag="IVY61_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7969405.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            35202..36605
                     /locus_tag="IVY61_000265"
     CDS             35202..36605
                     /locus_tag="IVY61_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7969406.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            36605..38038
                     /gene="ascB"
                     /locus_tag="IVY61_000266"
     CDS             36605..38038
                     /gene="ascB"
                     /locus_tag="IVY61_000266"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7969407.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            38183..39109
                     /gene="trxB"
                     /locus_tag="IVY61_000267"
     CDS             38183..39109
                     /gene="trxB"
                     /locus_tag="IVY61_000267"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP7969408.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            39322..41049
                     /locus_tag="IVY61_000268"
     CDS             39322..41049
                     /locus_tag="IVY61_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP7969409.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            41122..41415
                     /locus_tag="IVY61_000269"
     CDS             41122..41415
                     /locus_tag="IVY61_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7969410.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAIIQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(41499..42359)
                     /locus_tag="IVY61_000270"
     CDS             complement(41499..42359)
                     /locus_tag="IVY61_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP7969411.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            42451..42879
                     /locus_tag="IVY61_000271"
     CDS             42451..42879
                     /locus_tag="IVY61_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7969412.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            43041..43217
                     /locus_tag="IVY61_000272"
     CDS             43041..43217
                     /locus_tag="IVY61_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP7969413.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            43244..43690
                     /locus_tag="IVY61_000273"
     CDS             43244..43690
                     /locus_tag="IVY61_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP7969414.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(43846..45225)
                     /gene="brnQ"
                     /locus_tag="IVY61_000274"
     CDS             complement(43846..45225)
                     /gene="brnQ"
                     /locus_tag="IVY61_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7969415.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            45931..46902
                     /locus_tag="IVY61_000275"
     CDS             45931..46902
                     /locus_tag="IVY61_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP7969416.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            46926..49118
                     /locus_tag="IVY61_000276"
     CDS             46926..49118
                     /locus_tag="IVY61_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP7969417.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            49135..49611
                     /gene="ybeY"
                     /locus_tag="IVY61_000277"
     CDS             49135..49611
                     /gene="ybeY"
                     /locus_tag="IVY61_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP7969418.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            49589..49990
                     /locus_tag="IVY61_000278"
     CDS             49589..49990
                     /locus_tag="IVY61_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP7969419.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            50005..50904
                     /locus_tag="IVY61_000279"
     CDS             50005..50904
                     /locus_tag="IVY61_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP7969420.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            51047..51859
                     /gene="recO"
                     /locus_tag="IVY61_000280"
     CDS             51047..51859
                     /gene="recO"
                     /locus_tag="IVY61_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP7969421.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            52243..53160
                     /gene="glyQ"
                     /locus_tag="IVY61_000281"
     CDS             52243..53160
                     /gene="glyQ"
                     /locus_tag="IVY61_000281"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP7969422.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            53162..55237
                     /locus_tag="IVY61_000282"
     CDS             53162..55237
                     /locus_tag="IVY61_000282"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP7969423.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        17900 a         9923 c        11763 g        15679 t
ORIGIN      
        1 ctaatgaaaa aacttaaatt attcttacca ataataggat tagccattct tttatcaggc
       61 tgttcaaagt ggattgatcg aggagaatcc attacggctg ttggctcttc tgctttacag
      121 cctttagtag aaacagttgc tgaaacttac cagacagaaa atcctggaaa attcatcaat
      181 gtacaagggg gcggaagcgg aactggtttg tctcaagtac agtcaggagc agtcgacatc
      241 ggaaactctg atttgtttgc ggaagaaaaa tcggggatcg atgcctcagc attagtcgat
      301 catcgagtag cagttgttgg gatcactcct attgtgaaca aggatattgg cgtaaaagac
      361 atcagtatgg aaaacctgaa aaaactattt acaggcgaga tcaaaaactg gagtgaagtc
      421 ggtggaaaag atcagccagt agttatattg aaccgcgctt caggaagcgg aacccgtagc
      481 acgtttgaaa aatgggtatt ggacggagaa acagctgttc aggcacaaga acaagattca
      541 agcggaatgg tgcgccagat cgttgccgac acacccggtg caatcagcta tgttgctttt
      601 tcttatgttt ccgatgatgt agatacgttg aatgttaacg gtgtagaacc gacagatgaa
      661 aatgtgacaa cgaacaaatg gacgatttgg gcatatgagc atatgtacac aagagatcat
      721 ccaaaaaaac tgacaaaaga atttttagac tatatcttat ctgatgaagt acaagataac
      781 atcgttggag aactaggtta tatccctgtt tctcatatga aagtcgaacg tgattggcaa
      841 ggaaatatcg ttaaagagta atctttacac aaaatttaca aacttagcta aacttttaca
      901 tgttattaaa accaaattga tgttcaattt gtacactgag tgagtatgtt aaacctgatt
      961 aagggggagt catctgtgga agatgtgaaa aaagtattat taacaaaatc aaaacgttca
     1021 aaaatggaac agcgtggaaa attcatcagt tttttatgta tcgcgctgat cgtgttggtt
     1081 gtattatcta ttttttattt tgtagctagt aaaggattgg ccaccttttt tactgataag
     1141 atcaatgtat ttgattttct gtttggtaca gattggaatc caagccaaat cggagcaaat
     1201 gggaaaccaa tggtaggggc attgcctatg atcacaggtt cgtttattgt gaccttttta
     1261 tctgctatcg ttgccacgcc atttgccatt ggtgcagcag tattcatgac ggagatctct
     1321 cctaaaaacg gaacgaagat cttacagccg gtcatcgagc tgcttgtagg tattccttct
     1381 gtcgtttatg gttttatcgg tttatcagtt atcgtgcctt ttgtcagaag tatttttggc
     1441 gggacagggt ttggtatctt agctggtaca tttgtgttgt tcgttatgat cttgccgact
     1501 gtcacaacga tgactgttga tgcattaaag gcagtgccac ggcactatcg ggaagcttca
     1561 ttagcgcttg gtgccacaag atggcaaaca atctacaaag tcgtgctacg agcagccgtg
     1621 cccgggattt taacagcagt cgtttttggt atggctcgtg cgtttggtga agccttagct
     1681 atccaaatgg tcatcggtaa tgcagcattg atgccaacag gacttactac tcctgcttct
     1741 acattgacgt ccatcttaac aatgggaatc ggaaatacaa tcatgggaac agttgaaaat
     1801 aatgtgttat ggtcattagc gctgatcttg ctattgatgt cattattctt taatatcgtg
     1861 atccgtatga ttgggaagaa aggagagctg aaataatgaa cgcaaaacgc tcagataaaa
     1921 ttgctacagg tgttttatat atagtttcgg ggatcatcgt attgatttta gcagctttac
     1981 tactttatat cttagttcgg ggaataccgc atatttcatg gtccttcttg accgaacctt
     2041 ccaaagccta ccaagaaggc ggtggaatcg ggatccagct atttaactcg atttatttgc
     2101 ttttgatcac gatgatcatc agttttccta tttctttagg tgcgggtatc tatctttcag
     2161 aatatgctgg aaaaaactgg ctaacagatg tgattcgcac gtcgattgaa atcttgagct
     2221 cgttgccttc tgtcgttgtc ggtttattcg gtttcttggt tttcgttatt caattccagt
     2281 atggtttctc gattatttct ggtgcattgg ctttgactgt attcaacttg cctcttttga
     2341 ctagaacagt tgaggagtct ttacaagcgg tccattacac acaacgagaa gcaggattgg
     2401 ccttaggttt gtcacgctgg gaaacagtga taaaagtggt ggttccagaa gcgacaccag
     2461 ggattttgac tggtgtgatc ctaagttctg gaagaatttt tggtgaagca gctgcattga
     2521 tctatacggc tggacaaagt gcacctgcgt tagattttac gaactggaat ccattaagtg
     2581 tttcaagccc gatcagcatt ttccgtcaag ctgaaacatt agctgtccat atttggaaga
     2641 tcaatacaga aggcacgatg cctgatggtg tttctgtttc ggctggcgct tctgccgtgt
     2701 tgatcattgc tgttttactg ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc
     2761 gaaaaatgac ttcagcctaa ggagaagaag caaaatgaaa aaatataact ggaatgaaac
     2821 aaatgtcatc accatgccaa aagagaaaga agtagcacta tatacggatg atctacatgt
     2881 ctggtatggc gacaatgaag caatcaaagg agtggatctg gaatttgaaa aaaataagat
     2941 cactgcactg attggtcctt ccggctgcgg aaaatctacg tatcttcgtt cgttgaatcg
     3001 aatgaatgat gggatcgcta atacgaaaat cactgggaaa atcatgtatc aaggcgtaga
     3061 tattaatgcg cctcaagtgg atgtttatga gatgcgcaaa cggattggca tggtttttca
     3121 acgtcctaat ccgttcagca agtcgattta tgacaatatt acatttgctt tgaaacagca
     3181 cggcgaaaaa aacaaaaaga aattagatga aatcgttgaa acaagcttga aacaagctgc
     3241 cttgtgggac caagtaaaag acagcttgga caaaagtgcc ttggctttat ctggcgggca
     3301 acagcaacgt ttatgtattg ctcgtgcgat cgcgatgaaa cctgatatct tgctgctaga
     3361 cgagccggca agtgctttgg accctatttc gacaggaacg gttgaagaaa cacttgtaaa
     3421 cttgaaagaa gactatacga tcatcatcgt gacgcataac atgcaacaag cagctcgtat
     3481 cagcgactat actgcctttt tctatctagg aaaagtaatg gaatatgaca aaactaacaa
     3541 gatatttacc agaccaaaaa ttcaggcaac cgaagattat gtgtccggtc attttggtta
     3601 agacggaggc ctcaacatga caaaagaaat tatttcatct aaagatcttc atctgtatta
     3661 tggaaagaaa gaagcactaa aagggatcaa tctaagtttc aatcaagggg aaatcactgc
     3721 gatgatcggt ccttctggtt gcgggaaatc cacttatctg cgttctttga accggatgaa
     3781 tgacctgatt ccgaatgtaa cgatcaccgg aagtgtttta tataaagaca aagacatcta
     3841 tggtccaaaa acagatgttg ttgaactaag aaaagaaatc ggcatggtat tccagcagcc
     3901 aaacccattt cctttttcta tctatgaaaa tgtcatctat ggattgaaat taaaagggga
     3961 aaaagataaa aaagtccttg atcaagtagt tgaagaaagc ctgaaggcag cgtctgtttg
     4021 ggatgatgtg aaagacaaat tgcataaaag tgctttatct ttatccggtg ggcagcagca
     4081 gcgtgtatgt atcgcacgtg ttttggcagt taatccggaa atcatcttat tggatgaacc
     4141 aacaagtgcg cttgatcctg tttctactgg gaaaatcgaa agtatgctgt tagaattaag
     4201 agagcggtat acgatgatca tcgtgaccca taatatgtct caagcttcac gtatttcaga
     4261 taagacggca ttctttttag atggtcactt gatcgaatac aacgatacga agaagatatt
     4321 catgaatcca gaaaaacaag agacagaaga ttatatttca ggtcgatttg gataaggagg
     4381 aaacggaatg ctacgtacac aatttgaaga agaattgctg aatctccata atcagttcta
     4441 cgaaatgggg atgatggtga gcagcgccgt gcacaagtca gttcgtgcat acatcaaaca
     4501 cgataaaatc ttagcccaag aagttatcga aaacgatata aatatcaata acatggaaac
     4561 acgcttggag aagaaaagtt ttgaaatgat cgcattgcaa cagccagtta caactgattt
     4621 gcgtatgatc attacggtca tgaaagctag ctcggattta gaacgtatgg ccgatcatgc
     4681 ggtgtctgtt gctaaatcca cgattcgcgt aaaagggcag acgagaatac cggaaatcga
     4741 aaaagagatc tccgatatgt ccgattatgt gaaaaaaatg gtggacaacg tcctagttgc
     4801 ttatgtcaaa acagacgaaa aagacgcacg tacgatcgcc aatatggatc aaagagtcaa
     4861 tgactatttc aatcgaatct ataatgcaac aatcaaaaat atgcaggaaa atcctgagac
     4921 agtcatcagt ggaacagact acttgaatgt tgccagttat ctagaacgga tcggagacta
     4981 tgtgacaaac atctgtgaat ggatcgtcta cttggctaca gggaaaatca ctgagttaaa
     5041 tacgaaccac aatgagattt tctaaactga agctgtgatt atgccgtttg tttaggaaaa
     5101 gaggttgtga aatcagcggt tgtgaaaaag ctgttctgca tcaagtaata agaacagtca
     5161 aacaaaaaat agctcctcat atttttcgtt tggctggact tattaccgca gatgcaagct
     5221 tttgaacacc gtttattcgg gaaagggttt gtgacaaaac atgtcacaaa cccttttcat
     5281 catttcatag aaaggataaa gaatgcaaaa aaaaattagc atcatcacta tattttttat
     5341 ttgtgtgctt acttcatggg ttattcagca tcaagctgct ttatatgaag tacctgtcat
     5401 cgagatcgaa aaagtacgaa taaaagagga tcatcaattg attacaggaa gactgatgaa
     5461 cacggatcaa aaaggagaat cgatttattt atcggttcct tatcaaaaaa atcaaataga
     5521 aacagcaaat ataaaagcga gacaaatgta tctagtccaa atgcatggag atgaatggca
     5581 gctagtagca aaaaaaaaat gatggctggc ttttcttctt cacaagtatc tccattggcg
     5641 ccatgttgct tattggcgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
     5701 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
     5761 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
     5821 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
     5881 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
     5941 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
     6001 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
     6061 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
     6121 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
     6181 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
     6241 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
     6301 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
     6361 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
     6421 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
     6481 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
     6541 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
     6601 cctactattg cttcctatca ctgatttttt agcgatccaa ggttttacat cggtagaact
     6661 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
     6721 gttgcttctg attagtctta gtggtgctgt tttagatgct agtgtagcaa tttctagtgg
     6781 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
     6841 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
     6901 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
     6961 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtctgc
     7021 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
     7081 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
     7141 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
     7201 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
     7261 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaataa aaaaagaagc
     7321 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
     7381 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaaggcaaga
     7441 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
     7501 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
     7561 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
     7621 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
     7681 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
     7741 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
     7801 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
     7861 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
     7921 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
     7981 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
     8041 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
     8101 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
     8161 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
     8221 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
     8281 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
     8341 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
     8401 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
     8461 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
     8521 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
     8581 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
     8641 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
     8701 tgaaatcacg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
     8761 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
     8821 atagcttccc ctattttaca aaattgtgcc ttattgccga taggctatct cttgaacacc
     8881 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
     8941 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
     9001 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
     9061 gacatgatga aaaaaaagtt gacaaaatcc aataaaaatg ttgttttgac agggacttta
     9121 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
     9181 ctgagcttag ttgcttttgg aagtccaatc attttataca ttttattagc tttgattatc
     9241 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
     9301 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
     9361 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
     9421 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
     9481 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
     9541 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
     9601 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
     9661 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agtgaacata
     9721 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
     9781 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
     9841 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
     9901 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
     9961 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    10021 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    10081 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    10141 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccggctcc agaagaaatg
    10201 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    10261 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    10321 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    10381 aaaagtgaaa cagcgttgga gttgatcaaa cgcggtcatc gattgatcgc agacgatcga
    10441 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    10501 catctaattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    10561 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    10621 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    10681 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    10741 gcgatgaatt tccgtgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    10801 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    10861 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    10921 tcatcgtatc aggtatcatt atcgccatgt ggcttagcag cagagaggct gttcgggtag
    10981 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    11041 gtgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    11101 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    11161 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    11221 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    11281 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    11341 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    11401 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    11461 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    11521 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    11581 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    11641 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    11701 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    11761 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    11821 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    11881 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    11941 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    12001 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    12061 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    12121 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcgaaaca tgacatcact
    12181 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    12241 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    12301 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    12361 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    12421 ggtgccaacc cgctgacctt tatcggatta agcggagtag gcgatctagt cgttacttgc
    12481 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    12541 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac tcaagcagcg
    12601 atggaactag cccaaataat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    12661 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatgggaaa
    12721 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagtcaaaa
    12781 aagcagttat tccagcggca ggattaggaa caagattttt accagcaaca aaagctatgg
    12841 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    12901 tgaaatcagg aatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    12961 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    13021 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    13081 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccgtttg
    13141 tcgttatgct aggcgatgat atcatggaag atgaagtacc attgacacag caattaatcg
    13201 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    13261 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    13321 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    13381 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    13441 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    13501 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    13561 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtat atcctgtctt
    13621 taggtaaaaa attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    13681 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    13741 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    13801 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    13861 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    13921 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    13981 acaaaagacg tagatctttt gtctagacaa gtagaaggct tgctggtcaa aagtaatgaa
    14041 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    14101 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    14161 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    14221 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    14281 aaaaaggtgg atttttatta ggtgcagtag tcggaggaac agccgcagca atcgccgcgt
    14341 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    14401 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    14461 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    14521 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    14581 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    14641 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    14701 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    14761 aagcaaaacc agttgcagat gacacaaaag aaatgatgaa aatggcaaaa gaagatgcga
    14821 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    14881 actaaaatca caaatttata gaataaaaag gtgggagcgg actagctctc acctttttta
    14941 ctcttatttt tctaaaacta gtaattcttt tggtgtagtc gtgaatggca cacagccatc
    15001 gcttgtcaca tatacacagt cttcgatacg tacccctact ttttcaggaa tatagattcc
    15061 tggttcaaga gagaagcaca tcccttcttc gattactaag tcatttcctt ctacaaggga
    15121 agggaattcg tggacggttg ttccgatccc gtgacctaaa cggtgattga aatattcgcc
    15181 gtagccatag ctgttgatca cgtttcgtgc gatttgatca agttcacctg ctgtcacacc
    15241 tggacggacg gcttcttgtg cggcaagctg tgcttcaaga gtgatgttgt atattttttc
    15301 ttggaagtcg gttggttttt gataagcaat tgtacgagta gcatcactgc aatagccatt
    15361 ccaaacaacg ccaagatcga ataacacaag ttcattcggt gaaatcttcg tgtttcctgg
    15421 tgtaccatga gggcttgcag catttgctcc tgcaaggact aatgtgtcga aagacatttg
    15481 tgaaacacct tgttttttca attggtattc gatttctgca atgatcgctt gttcagtcac
    15541 gccttctttc actgctttaa agccaatctc aaatgcaaca tcggcccatt taccagcttc
    15601 gatcagtttg tctttttcct ctagagtttt gatcagttgt agacgttgga tgatggaagc
    15661 tagatcagtg taagaatccg ttcccgtcaa gatatcagag atagcatcga agcgctcgac
    15721 agtcaaggcg ttcttttcaa tccctacatt gccaggctga ccatagcgag cacgaagttc
    15781 ttttccgatg atctcccaag ggttttcgct gtctaaatag ccacggacat catacgccca
    15841 actactgttt tgagcatctt caacttccaa agcaggagtg aaaaggaaag gatcttgttc
    15901 aagagggata aaaagagcta gtacacgctc atgaggatca ctttcgtatc cggaaaagta
    15961 agcgatgtgg ccagggtcgc tgatataagc caattcgact tggtgttgtg ccatccattg
    16021 acgtagttca ttgattttta cgttattcat ttggcaacct actttctaaa ttaattttgt
    16081 attcaataat tgtaccactg atttattaaa agaccaacgt tctaaaaact gaaaaaagta
    16141 aaaattttca tgaaaacaga gataaaacgg ttgcaattct tgagattatt tgatattcta
    16201 ttttagaaat gtaagacgta ttttcagaaa acatataaaa tatgaatagg agaacacaaa
    16261 tggaaaagca aacaatcact atctatgatg tagcgagaga ggcaaatgtt tcgatggcta
    16321 ctgtttcacg tgtcgtaaat ggcaatccaa acgtcaaacc agcaacaaga aaaaaagtct
    16381 tggaagttat cgatcgcttg gattatcgtc caaacgcagt tgcgcgtggc ttggcaagta
    16441 agaaaacaac aacagttggg gtaatcatcc ctgatgtcag caatatgttc tttgcttctt
    16501 tagcacgtgg tatcgatgat gtggcaacaa tgtacaaata caatattatc ttagcaaatt
    16561 cagatggtaa cgaccaaaaa gaagtcaatg tattgaataa cttactggct aagcaagtgg
    16621 acggcgtgat cttcatgggt caccatatca cagacgaaat ccgtggagaa ttttcacgtt
    16681 caaaaactcc tgttgtatta gctggctcta ttgatccaga tgaacaagta ggtagcgtaa
    16741 acattgatta tgtatcagca acaaaagatg cagtaaacaa attggcaaaa agcggcaata
    16801 aaaaaatcgc ttttgtcagt ggtgcgttga tcgatcctat caatggacaa aatcgtctga
    16861 aaggctataa agaagcatta agcgaaaata acctaccata tagcgaagga ttgatctttg
    16921 aagcacaata caacttcaaa gatggtctat ctctagtcga tcggatccat aatagcggtg
    16981 caacagctgt ctatgtttca gatgatgaat tagccatcgg tattttggat ggtttattag
    17041 accgtggcgt gaaagtaccg gaagaattcg aaatcatcac aagtaacaat tcactactga
    17101 cagaagtagc tcgtcctcgt ttaacaagta tcacacaacc attatacgat ttaggggcgg
    17161 tatctatgcg cctattgacg aaaatgatga ataaagaaga agtcgaagaa aaaacgatta
    17221 tcttgcctta tggaatcgaa gaaaaaggat ctacaaaata ataaaaggac aaccattcag
    17281 aggttgtgag cctgccgttt aactctgcgt tacaaggagc aatttcaaaa aacagcattt
    17341 catgtttgat gaatattgtg actcgtgatt cggagttggc aggagaacac cgtttattcg
    17401 ggaaaagggg ttatgacaaa attttgttta atcctttttt tttgtcttaa aaaagagaga
    17461 aagtggttga aattacattt atcgtatata aaacaatccg tgtatagatg ctgcattatg
    17521 ttcaccacca tcaaaaaaat aagggactgt gtattctatt aaatcaatca tctttctcat
    17581 ctgattcttt ggttgcttga ttcgctcttg cataagctga agcggtgttc cagtaatctg
    17641 tatagttttc atcggtaccg ccaacgccaa atttaaaggc gcttttcttc ggtccatttt
    17701 tcgcccatag cgattcaact gtttttcctg gtgtttggat ggatttttta ttgacagtaa
    17761 cttttccagg tgtttgacca gtaaatccag atactttttt cttcatcaca tcagatgata
    17821 attcgaattt ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct tgattgatca
    17881 gattggcaag ataattagcc gaacgtatcc ttgctttgct gtccattcca gtattgtcat
    17941 cgtgccctgc ccagctactg atggtaatag acggagtaga gacaattagc catgaatctc
    18001 ggtattcatt ggtagaacca gtttttccga cccaatctgc ttttcctaga tttccgttta
    18061 agcttgagat ggcatctttg aatggtgtag tgatttttgc attgatcaca ctgcgcatca
    18121 tgtcattcat gatcgatgcc gttgcttccg aatagatccg gacagagttg ctttcgtgtt
    18181 tataaattac atttcctgcg ttatctgtga tggaatcgat gatatagcct tcttcataga
    18241 cgccgccgtt tgctaaagcc tgaaatccat tggtctgttg aagcgtcgtg acatctactg
    18301 tacctaaagg agcagattca acgccccaat tgtcatttgc cgggtagttc atctttgcca
    18361 agtagtgctc gtaagcatat tgtttcgatc caccggaatt caacacatct tgatataagt
    18421 gataagccgg gatattgttg gaccattcta gtgactcacg gacggtttgg aaggtgttcg
    18481 atccttcgtt agtagcattg acgattttct cgccagcatt ttctccttct tgccaagttg
    18541 ttgcataatc tgatacacgg gattctgatc cgataagccc catgtcgatt gcaggtccat
    18601 aaacaagaac tggtttgatt gatgagccag cttgacgttc tgtatcgaag gcatggttat
    18661 tttgattctg actgtaattc cgtccgccaa cgaatccgta gatgcggcca gttctgttat
    18721 ccatcaatac atttccggtt tctacttgac tgccagcacc atcatctaac atgtatccat
    18781 aattttctac gccattttgc aatgcagcat agacatcttt atcgattgtc gaatggattg
    18841 tatatccttt attttggatc gtttgctgtg ccttttgata ataagcgtca ctgaccgatg
    18901 aatcagacat atcagcgttg tctttttcag ccaattgttg tgcaatgatt cttgttgctt
    18961 cattcatcac agtataatac aagaattctc tgtcagtctg ttctgcgatt tgttgtggta
    19021 gaaagtcttt tgtaaggtca taggcttttg cttcttcgta ttgttctttc gtaatttggc
    19081 cttcacgata catactgaat aaaacaaaat cttttctggc aaggccggca gaaagatctt
    19141 cttttagcgc acctgtgttc gtgtatggac tatacgtgat cgggctttgg ggtaatccag
    19201 cgatataagc agcttgcggc agcgtcacat cttttgcatc tacaccaaag attcctcttg
    19261 cagcttcctg gactccagca atgttttgtc ccttattatt acgaccaaat ggtgagacat
    19321 tcaaatacgt agcaatgatc tcatcttttg aaaagtattt ttctacttgt gcagaaagaa
    19381 tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt aaggatctgc tgtttgacta
    19441 attgctgtgt caaagttgat ccaccagaag agccaatacc agtagcttca gatactaatg
    19501 ctcgtaaaac agctttagga acgtaacctt ggtgtttatc gaagtattca tcctctgtgc
    19561 tgataattgc agttttcaat aaaggtgaaa ttttatcaga tgaaatagtc gtgcgcatca
    19621 gatctgtctg gatttttgat atctctgtat tatctgcata aacaatcttt gacgtttctg
    19681 taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg tcttcgacga
    19741 gataggcaaa atagccagcg cctattccaa tacctagagc tgcgaacatc aaaagaagaa
    19801 cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat aatcttagga acaaaccatt
    19861 tttctttttt tcttctattc tgtttttttc gagtattttt gcgtggcaaa agggtcacct
    19921 caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa cagacaaggc
    19981 tgtttttggc aggaatttct tttttaaaag agttaaaacg tgagaaaatg atctttttta
    20041 taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag aacagggcaa
    20101 agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat tgttagtaac
    20161 aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag catgagcgga
    20221 aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc gacttttatg
    20281 atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat gaatattgat
    20341 gccggaaaag cgtacatggc agaaaacggt ctaacaaata tcgtcgttca tgccccttat
    20401 atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt ccaatttctg
    20461 agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact tcacccaggt
    20521 gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg cttgaatgaa
    20581 gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg taaaggtacg
    20641 gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa attgaatgaa
    20701 aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa tattaaagaa
    20761 gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg gcttaaagta
    20821 ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccggca cgccaatatt
    20881 ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc aatgtttgac
    20941 aatatttcga aaatcttgga aactccctat gtcggcaaag ataaaaagaa agcttacgca
    21001 ccatatggaa aagaaatcgc catgttcaaa gcacaaatat tcgatccaga agtgtttcct
    21061 gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag caaaaaagga
    21121 ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtgcttt ttgcagtctt
    21181 tttcatcatg catcttctcc cccctctttt taaatttaga caaacagggt atactggtag
    21241 aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg aaggacgaaa
    21301 agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat acatacttta
    21361 ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt aaagcaatct
    21421 atggaacaga catgtcggca acctatgatg accagctgct gattcgctat gctcaaaata
    21481 ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa ggaatctcct
    21541 acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt ttgttcggca
    21601 tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatc gtgaagaaca
    21661 agtatcttct tttaagtttc tatttttatt ttttgttttc tccgattagt tttacacatg
    21721 aatattccac tcgaacgtat cgtaatgccc tcgtgattcc ggctgttgag atcattatcg
    21781 cttgtttact agctatttat taccgtaaat atagttccag caaaaaaatg ctgccgtggt
    21841 ttttagggct agctgtttct tttcctttct tttggttcat tcgcgaagat tccatctggc
    21901 ttcttccttt tactgtagtc gcgttaggca tcagtttcgt ccagatcttg ctaggacaag
    21961 acttttcttt caaagaagca aagaagttaa aagaacgacc atttctcctt tcgaaacaag
    22021 aatggctgaa actttcattg tttttactcc ctctagtcgg tttctccatc gtctatcagt
    22081 ggatcgaata taaaaacgag acaacatata gtattgccgc catcaatgac cgttcgagtg
    22141 gcgaatttgg acgtttgatg aaacagctga tccgaataga tgacgaaacg aacttaaata
    22201 aagaaaacag tgagatatgg gtctcgcatg ctgcattaaa taaagcatta gacgcatctc
    22261 ctacatttca tgctgtttct actcgtattg acgaattgta tacgactcat gcgtggaccc
    22321 aaggtggaaa agtaaaagaa ttaccaggag atatcatttt ttgggcattg cgagatgtgg
    22381 taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt tggaaaaaag
    22441 tcaatcaaga attagaagaa gcctatcaaa agggaactat caaaaagaaa aaagaattct
    22501 atctgatggg atcaggggat ggaaagacga tgaatgatct gccaatcgta tttgcttttc
    22561 tgaaatcagg atatgcctat gcatggcgat acaaagattt ccatcaaggg gggaattata
    22621 gtttcggtac gcaagaacaa gtcgaacagg cccaagaatt attgaatctt ccactaatgg
    22681 ataattggat cgatagcagt gatccgagaa cacctgattt ccagttgaca aaaacagcaa
    22741 aagtcgcgaa tgtcatgata aaaatttacc aagttctggc cattcccgtg ctgattgctt
    22801 caatagcagg gatattactt atgatactag gaaccgcatt tgcaaaagct gatcgtgcct
    22861 attatagttc ctctttgatt gtagtcaccg gattaatact gacacaattg gtttttcttt
    22921 tcggcgtctc gtggttttgt tcttatgcgc cagaaaaaaa ggattatttt ttaagtgtct
    22981 acactggcgc aggcgtgcca ttaacgcaat tcatcgtagt aacaagcatg ctagttttca
    23041 gtaaattgaa tctgaaaaaa gctttatctt ccaaataaaa acatactaag attagaggag
    23101 aacatcattg acgaatgatg ttctcctcta ttttctattt ttagattata gtgaagagtg
    23161 aaagaaagtt cgaacaaagc cttgaggact atgagtccct atcaaaaacc gaacttcttt
    23221 actgtcattt tagaatcagg tttacgtatg ttggggccat gagcccgtgt ataggaaatt
    23281 ggaatcgaaa tgaagcagta aagttctttc aaaacttaaa aaggaggaaa acaaatgcct
    23341 tacattcttg cattagatgt ttcaatggga cacagctaca gtgtcctata caaagacgac
    23401 acctgcttat ttgaagacga gatcgaacac actcaaagag gatttcatgc attactcgaa
    23461 gagatccagc acttgcctga acgtccattg attgtttttg aagcaacggg gatttattcc
    23521 agaccagtcg aaaagttctt tcaagatcat cacttttctt actgtttgtt aaacccgtta
    23581 gaagcgaaaa aacagatgga agaaaacacc ttatgcagtt ggaaaacaga taaatcggat
    23641 gcccatcggc tcgcacagac tcatcttaaa aatcaacgtc agccaaaaga agtacaaagc
    23701 aatttttact tagagatgcg cgatttagcc cgtttttatc aagaaattga gaagaaaatc
    23761 actcgtctgc gcatggattt acacaactgc cttcagttga cttttccaga gcttgaacag
    23821 ttctttttaa acaggctgac cccttacgca ttgatattga tccgtctttt tccccatcca
    23881 gattttgtat tggcatctac tcggacaaaa ataaaaaata aactgatcaa cgaaacacgg
    23941 aaaaaaatct ccgcgaatcg tgccgagcaa aaggcagatc aaatcatcca ctatgcacag
    24001 tgtgcctatc cagctgttga aaaagacagt atccactgtc aaaaaacgat ctattacgct
    24061 gaacttctcc aagatttact cgaacaaaaa gaagcactgg cgacacaaat gatcaaaaaa
    24121 gccgaaggct ctccttgctt tttgttgtac caaacgttcc ctgggattgg cgcactcaca
    24181 gccgctttat tgctcggtga attaggagat atcacacgat ttaaaaccca taaacaattg
    24241 aatgcctttg tcggaatcga catccgacgg tatcattcag gtaagtatac tggccaagac
    24301 cgcatcaata agcgtggtaa tcccaaagca cggaaaatta ttttcttcac cattcgaaat
    24361 atgattcgcc agcaacgcgc agctcctaat catattgtcg attattacta caaattaaaa
    24421 aagcaaccta tccctaagaa agataaggtt gccacagttg cttgtatgaa caagcttctg
    24481 aaatgtatgc acgctatggt tagggcacat acagagtacg actatgcgta cgcggtctca
    24541 gtggaccatt aactaaacca cttacagtat accacttctt tgcccagatc gaaaattttt
    24601 tacgaacggg gctttatttg gtgtgccttg attttgtcgc tttcgtttat gagctatttt
    24661 tcttacacct tttacttgac taatcgtagg aaagggaact gtgacaaaag tcttgtcgca
    24721 gttccttttt ccgaataaac ggtgctcaaa agcttgcatc tgcggtaata agtccaacca
    24781 agttaaaaac ataaaaagct gtttttactt ggctgttctt attacttgat gcaaggcagc
    24841 tttttcacaa cctctgatac acggtgttcc agaagtaact tcttcggaaa taagccgaaa
    24901 tttcacaaaa atttgaaaag caattttctt aaattccttc ttatttcttg aagctgccca
    24961 cttctgacac aacctccttt gtgtaaaaga atcagtttct actcgatttc ttctatcgtt
    25021 tcctgcaaac gctgttcata ttttttgttt cctttatata aatcaccaat cgtccatgcc
    25081 aacaggagaa ggacaagaac aatgattaca taagcgattt gatcagccaa tgctagttga
    25141 gctgaggtgg cttgctcgcc gaagaaactt tcgatttgac ctggtaatcc tcgtaaattc
    25201 aagtaagtca gactaatcac agagaaccag ccaagtagtt ttacaacaaa tgaatttttg
    25261 aatcggctcc ccatctccgc ttggctattg gtcatcatca gtaaaggaat catcgagaat
    25321 ggcaatgcaa aagccagaaa aacttgggat tcattcatca agttattcaa agcggtatgt
    25381 tcttcgatcg ttccttttgt acttgtaaac aaaacacaaa tcaatacagg aatcacagaa
    25441 agcaatcgtg tgactagtct tcttagccaa attgggatac gcatatggat gaagccttcc
    25501 atgatgactt gaccggtcaa cgtaccagta atagtagagt tctggccaga agcaagaagt
    25561 gctacagcaa ataaagtaga caagatacca gattttgcta cagcaatcaa gataccattg
    25621 ctcatagctg atggatcaga taaagcttca tacagaccaa aaaaggaagg atcttggaca
    25681 gaaccggttt tgaatacagc cacacccatg atcaatagca gtgcattgac tagaaaagcc
    25741 attgacaact ggatattaga atcccaagta gaaaagcgta ccgcttgtgc tacatcttct
    25801 tcgtcttggt gatcgatctt ccgagtttga gaaactgctg aatgaagata taagttatgc
    25861 ggcatgactg ttgcaccaat aatccctaaa gctccagtca gcggagtttc gccgccgatg
    25921 accggtgagt ctgagaatgt gtgactgtta gggataaatc cggcaaacaa actgtgccaa
    25981 tcagggttag acaagccaac ttgatagacg aaaacgaata aaatgacgaa aatcaagcag
    26041 acaaccaaag cttcgatttt acgaaacccg acttttgtca gcaataaaag gacgaaaaca
    26101 tctaacacag tgataaatac ggcaattacc aatggaatat caaaaagcag gtagagggcg
    26161 attgctgcac caatgacttc tgcgatgtct gttgccataa tggctaattc agtcaagatc
    26221 cataaaatga cacccaatgt cttacttgtc cgtgctcgga ttgcttgggc caaatccatt
    26281 tgggtcacga ttcctaattt tgctgccata tattgtaaaa gcatagcaat cagactagac
    26341 atcaaaataa ctgacatcaa taaatattgg aaattttgtc caccagtgat cgaagtggac
    26401 cagttacctg gatccatata accaactgct actaatgcac ctggtccaga gtaagcaaga
    26461 agtgttttaa aaaaactttt tcccttgggt acatccactg ttccatttac ttcagctaaa
    26521 gataaaccat ttgtatgttc aataagtcga tgcttatgaa attgtttgtt ggattgatta
    26581 ttcaagaaaa aaccaccttt cctatttatc tacgattaca ttctacacct cagaaagaaa
    26641 aaattaaaga aaaagatttg gtatgcctaa ttttttttcg tggcgtgtct tgctggttaa
    26701 tgaaagaaat tttgttcttc tctaaaaaaa ccactatttt ttgcatgaaa ctaaagaata
    26761 cgcctttctt ttttcaataa actttagtaa aataaatcat tgagagcaat tcggaaatag
    26821 aaaggagaga tttatatggt atcaacaggt attgtcgtac ttattgctat tattgctttg
    26881 ctaatcggcg cagtaggtgg ctttttccta gcgcgtaaat atatgaaaga ctatttagaa
    26941 aaaaatcctc ccgttaatga ggacatgttg cgatcaatga tgatgtcaat ggggcaaaaa
    27001 ccatctgaga agaaaattcg ccagatgatg cagcaaatga agaaccaagg gaaaaaatag
    27061 gttctgaccc aaagactttt tgcgggctga ttttttcagt ccgtttttta tttcgtaatt
    27121 tgttagacta ttcagactat tcagcaaaaa agacaagcac agacaatcct atgctcttga
    27181 taaagacaaa ggaggagtaa aatgtcgatt tttagaaaat taggttggtt ttttcgacag
    27241 gaaaagaaac attatttaat tgggattttc tccctattcg cagtggcctt ggtacagtta
    27301 gctcctccaa aagtgattgg gatcatcatt gatgagatcg ccgataaaaa tttatcaata
    27361 aagatcattt cctggtggat cttattattg atccttgcag ctctcttaca atatcttttt
    27421 cgttatatct ggcggacgaa tatttgggga agtgcagctc ggttggagaa agaacttcgg
    27481 cagcagctat tcaaacattt tacagaaatg gacaatctct tttaccaaaa atatcggact
    27541 ggagatttga tggctcatgc aacgaatgat ttgaatgcaa tccaaaatgt agcaggagct
    27601 ggtatcttga cctttgcaga ttcagtgatc acgggcggaa caacgatcat tgcgatgatt
    27661 ttgtttattg attggcgctt aacactgatt gcgctgattc ctctgccttt actagccgtt
    27721 acttcacgag tgctggggtc aaaactccac gatgcttttc gtgattcgca agaagctttt
    27781 tctagtatca atgataagac acaagaaagt attactggga tcaaagtcat taaaacattt
    27841 ggacaagaaa aagaagatct cgcggatttt gaagaaaaga ttgaagatgc aattattaaa
    27901 aacaaacgag tcaatttttt agactcctta tttgatccat tcattacctt gattatcggt
    27961 ctgtcttatg ttgcaacaat tattttaggt ggaaagtatg tcttggaagg cacgatctca
    28021 atcgggcaga tggtctcatt tattagctat atcggtatgc ttgtttggcc gatgtttgcg
    28081 attggtcgat tgttcaacgt attggaaaga ggaaatgctt cttatgatcg cgtcaatgaa
    28141 cttttgcatg aaaagacgca tatcgtggaa caactgggtg cagtgcagac tccggcaaaa
    28201 ggcgagctga atgtagctat ttcctctttt aactatccga aagaagaatc accagcatta
    28261 gaacaaatca aatttgcgat ccaagaagga gaaacattag gtattgttgg gaaaactggt
    28321 gcgggaaaaa cgacaatcat caaattactg atgcgtgaat acgatgatta tcaaggaaaa
    28381 attcaatttg gcggatgttc gatcaaagaa tattcattga atgctttatt aggatcaatc
    28441 ggctatgtgc ctcaagatca cttcttgttt tcgatgacca ttcgagataa tatccgtttt
    28501 tctaatccaa aactttcaga agaagaagta gagcatgcgg cttctttagc atttatcaac
    28561 aatgaaatca aggaattacc aaaaggttat gataccttag ttggagaacg tggagtctct
    28621 ttatctggcg gacagaaaca aaggatttcg attgctcgag cattgatcaa aaatcctgaa
    28681 ttgctgatct tagatgatgc actttctgca gtagatgcca aaacagaaga agctatcttg
    28741 cataatttaa aagaaataag aaaagagaaa acaaccatca ttgcagctca ccgtttaagc
    28801 agcgtgatgc atgcaaaaga aatcattgtt gtcgatcaag ggaaaatcat tgaacgagga
    28861 acccatcatg agctgcttgc attaggcggc tggtatgaac gaatgtggga gaaacagaaa
    28921 ttagaagcga agatagaagg aggcgaggcc tgatggatga aaattatcaa tcggaatgga
    28981 caaaatcgat tccacttaaa gaacagaaga agatcatcaa acgactgatg aagttcgcca
    29041 aaccttttag aagaacgttc atcgttgcta tcttatttgc gtttgccctg tctgtgatca
    29101 atgtcctatt gccaaggatc atccaaactt ttatggatga tcatctggct aaacagtcgg
    29161 ctacgacaca agttattttg ttttttgctg gtatgtactt gtttggagtc atagtcaaaa
    29221 gtatcatttg gtttttccaa tggtatttgt attccatggc ttcattgaag acctatcagt
    29281 atgttcgggt aagattattt gaaaagctgc atacactagg tatgcgttat tttgatcaga
    29341 caccggcagg atcgactgtt tctcgagtga caaatgacac agaaacgcta tttgaatttt
    29401 ggtatgtctt tttgatggta ttgactggga tctttgcagt cgtttcttcc ttcattgcca
    29461 tgtttcaaat caatggagaa attgccttgt ggactttggt gttcttgccg attttaggaa
    29521 tcgtcatctg gtattaccag aaattcagtt ctcgtattta ccggaatatg cgggaaaaat
    29581 taagtcagct gaacacgaaa ttgaatgaat cgatttccgg catgcgtatt atccagcagt
    29641 ttcgccaaga agctcgttta gctaaagaat ttgaacaagt gaacaacgag tatttagaca
    29701 cgcgctatgc gatgatcaaa acaaattcga ttctgttaag tccgatcatc aattttctat
    29761 atgcattggc gattgcgttg gcgttgactt tgtttgggat cgatgcgctg cattctcccg
    29821 tagaagttgg actgatctat gcatttacga cctatgtcca gggatttttt aatccaatgt
    29881 ctcaaatgat ggactttttg agcaccttta cagatggaat ggttgccggt agtcgtattt
    29941 tgaaaatcat ggatacagaa gaatatgcac cgcaacaaaa tcctggagca gatcaagcga
    30001 ttacttcagg taagatcgag tttcgcaatg taagcttttc ttatgatgga gagcacaatg
    30061 tattgaacaa tatctctttt gtcgctaatc cgggagagac cgttgcatta gtggggcaca
    30121 ccggaagcgg aaaaagctcg atcatcaacg tattgatgcg gttttacgaa ttttatgaag
    30181 gccagatctt gattgatgat catgacattc gagaatatcc aatggaagag ttacgtaaga
    30241 aaatgggatt ggtcttgcaa gatgccttct tgttttatgg agatatcgca ggaaatatcc
    30301 ggatgatgaa tccaactatc acagacgaac agattcgcca tgcagctgag tttgttcaag
    30361 caaacaagtt catcgatgaa ttaccaaaag gatatcactc aaaagtgatc gaacacggtg
    30421 ccagctattc tagcggacaa cgacagctga tcacttttgc gcgaacaatc gtgacagatc
    30481 ctaaaatact agtattggat gaagcgacag caaatatcga tactgaaaca gaaggattga
    30541 tccaagaagg attgacaaaa atgcgacaag gacggacaac gattgcgatt gcccatcggc
    30601 tgtccacgat tcgggatgca gaattgattt tagttttaga caaaggtcat atagtagaac
    30661 gtgggaatca tgaaacactt ctagcgcaaa atggtttgta tgcagatatg tacaaactgc
    30721 aaagcagtgg tgtgtaaaaa gtggttgtga cagggctccg ttcagcttta ggtgataaga
    30781 aaaaaattgt aaaaaaggct ttccagattt ttgcataaat ttggcttatc accgataagc
    30841 tggtactgga acaccgtata ttagaagttg ggacagtagc atagtagcat tttgacctaa
    30901 gcgctagagt gaaaataaaa cagagcttcc cagattcttc atctgtgagc gttagtgtcg
    30961 taattaccag atttaaaaaa aatcacgaca gctagtcaga agtgcccaat agccgctaag
    31021 aaagcgttga caaaagtgct tgctttcgat aatataaaga aaacgataca acttggatag
    31081 tgattgcctg atgcaagcta aacctgatga gtctcccatg gttccatgga cggctttttg
    31141 ggtttttctt tttgttttgg atacagccct atatatcatg gaaaggagta aaacatgctg
    31201 attgagaaag tactgaataa caacgtcgta ataaccaggg atgaaagaaa tcaggaaatg
    31261 atcgtgatgg gaaaaggttt ggcattcaaa aagaaagtgg gtgacccgat ttttgactca
    31321 tgcatcgaca aaaaatttca gttgacaaat gacggacttt cacaacagtt ccaagaattg
    31381 gcacaagcga ttcctcttga gtacttagaa atttcttgtg aaatcattga atatacaaaa
    31441 gaggctctac aagtccagtt gaatgattcg gtgtatattg cattgacgga tcatttgtat
    31501 acggctatcg aacgtcacaa aatgggaata tttgtaccaa atgtattgct gtatgatatc
    31561 aaacaactat ttcctaaaga gtatgccatc gggaaaaaaa cagtgaagaa aatctcggat
    31621 aaataccaca tcaagttatc agaagatgaa gccggattta tcgcattgca tttagtcaat
    31681 gcacaaggca ttcaaagcca acaagacaat acctacctgt ttactacaac aatacaggat
    31741 attctaaata tcgtccgcta ttactataag attacatttg atgaagattc agtccatttt
    31801 tttcgattcg tcacacacct acgttttttt ctgacacgcg tcctagatgg agaaaaatca
    31861 gaagtgggag aagtttctga ggaattgtac caggttattc aaaaaaaata taaggatacg
    31921 tttggttgcg ttgagaaaat agcagacttt ttagccgaga tattcaacta ccagatgtcc
    31981 acggacgaaa aaatgtattt aacgatccat attgcgcgtt tagtgcatga gaatcgttgt
    32041 aaataaataa aaaaaggagg ataaattaac aagaacgcat gaaataaggg tagtgacatt
    32101 cagttggcta aaccttccac taataaattg gagggaatag aaatgggaaa atatcaagaa
    32161 ttagcgagca aaattgtaga aaatgtcggc gggaaggaaa atatcaatgg tttgacacat
    32221 tgtatcacaa ggcttcgttt caaattaaaa aacgaagaaa aagcaaatga tgagatcttg
    32281 aaaaatatgg acggtgttgt gacggtaatg cgagcgggag gacagtacca agttgtcatc
    32341 gggaatcatg tgccagtcgt gtttgaagag gttataaaag caggaaacct tacttttgat
    32401 gaggctgttt caacagaaaa aatgagaccg tttgatatgc ttattgatgg aatatcgggc
    32461 tgtttccagc cattccttgc tattctagct gctggtggga tgattcgtgg actgacagca
    32521 ttacttgtct tcttaggcgc atttgaccgc ggttctggca catttgttat gtttgataat
    32581 atcggtgatt ctgtattcca atttatgcct gttattatcg gtttaacagc tgcccgtaaa
    32641 tttaaagtaa atgaattcgt agggatgttg cttggggcag cattaatgaa tcctagcttg
    32701 tcgctggaag ctttgagtgg tgcagcagaa gcaccactga ctaccatatt ttctggcact
    32761 atttttgaag cacctattta tcaaacagta ttcggcattc cctggattgc gcgtaactac
    32821 gcttcttctg tcattcccat tatctttatt gtgttgttag ctagccaaat tcagaaacca
    32881 attaaaaagt tagtacctga gatgatcgcg aatttcttcg ttccattctt caccgttctt
    32941 attacaatgc ccttaggttt tttactcgtt ggaccagcat tcacattcgc aactgatatt
    33001 ttaatggctg gatttgaaac acttttagca ctttcgccag ttatttatgg agcaatcgta
    33061 ggcttcttct ggcaaattct agtgatgttt ggtctccatt gggcaatcgt accaatggga
    33121 ttgatgcaat tttctgtgaa tggttggcaa aatatcatga cacctgttgc agtagtgtct
    33181 tttggacaaa ctgcagcact tactgcactc tactttaagc ttagaaaccc aaaagacaaa
    33241 gcaatcgcaa taccagccat tgtttctggt attgtgggga ttacagagcc agctatttat
    33301 gggtttacat tgccacgtaa aaaaatattt atttttacct gtgtcggtgg tgcgttaggg
    33361 ggcgcgtata gtggattaat gaatttaact tcttggaatc caggtggatt aggcatcttt
    33421 acaattccca actatattag accagatggt gatttaacgg atgtgattaa tgtattgatt
    33481 ggaatcgcca ttgcgatggt ggtatccttt gcgctgactt ttttcttttg gaaggaggaa
    33541 gcaggtgaaa cagatgatag acagaaaaga tcaggtaaag aaattgtaaa aacaccgata
    33601 caaggacaaa tcgctccact gaatgcagca aaggatgcag cttttgcaca aggaacacta
    33661 ggtcgaggaa tcttgatcta ccctgaaaaa ggagaagtac gtgccccttt tgacggaaca
    33721 gtcatgaccc tgttcccgac gaaacatgca atcggaatgg tttctgaagc aggtctggaa
    33781 ttgctgattc atgtcggatt ggatactgtt cagttggaag ggaaatattt tgaaagcttg
    33841 gtgcagcaag gagaaagagt gaaacaaggc gatctgttgc tccgcttcga caaaaagaaa
    33901 atcgaagagg aaggatacag tttagaaaca cctgtgatcg tcaccaatta cacggattat
    33961 ttggatattt tggaaaatca aggggagacg gtcgggccag acgatgcact tatcaccgca
    34021 ctgacctgaa cggttaggtg aaagaatgga ctaggcttaa ttattctttt tagtccactt
    34081 ctttattttc aaaattgaaa ggctctttca tgatggctga gctgataggg cgtttatcaa
    34141 atttttgtcc tacagaaatt agatggacta aattctgttg ataattttct gcaaaaacac
    34201 aactatataa cacgtctaga agataacaaa tcgatgtatt gatcgtaaaa ttaccaattt
    34261 tcgaatacag tcgttcgcgt gtcgtaagtc gcaaagcggc tgtactcatt gaagatagcg
    34321 ttgaatcacc gatactggtc aaagctaaaa ttggaacatg attttgtttc aatatttctg
    34381 cggtacgaag gatcacattg ttttcacccg tataagaaat cagaatcccg cacatatcac
    34441 tcgtgcaatt gtacgcttca tagaacgatt caccaaaagt ggtgacaatc gtgacatcca
    34501 ttttgatgcg cttcattttc agcatgaaat cttgagaaat caaagtattc gagttactgg
    34561 cgaaaatcac gatttttttt gcttgaagca ttagttgttt ggctttttgc agttggtcat
    34621 gatgaatgag agaaagtgta tcttgaatcg tcatctgttc taaagaagcc attttatttg
    34681 cgatcgtcat aataccgtct gtggtggaaa aaggaagatt cgcatctacg tcttgaaaat
    34741 tcccttcaag ataggctgtt tcagctaaaa aagcattacg aaaactgacc cagccatcaa
    34801 aacccatttt ttttgctaca cggatgagtg tggacgggtg gacataacat cgctcagcaa
    34861 tctcttggat ggtcatttcg ccaagtgctg gagaatcgct taagagaaac tgaatcactt
    34921 cattttctga aggagaaaag gagatttctt tgattttttc acgtaataac atgtagatac
    34981 cttcttcttg tttttttacg taaattataa cttttttgaa aatgatttgt aaacggattt
    35041 atgttatata gcttaataaa gacatttttt gacaagatat ttacaaaaat ccagtagaaa
    35101 acactaactt ttgtaaatgt tactggaaag aaacgaattt ctattgtttg ttttgtttac
    35161 aaaaaagata ataaaagtgt aaaaagtaaa ggagtgagaa catggcaaaa ggaatcaaaa
    35221 tcgctacgat tggtggaggc agcagttaca cccccgagtt gatggaagga tttatcaaaa
    35281 gatatgagga attaccgata cgagaaatct ggctcgtaga tgtagaagaa ggaaaagaaa
    35341 agcttgagat cgtcagtcag atgtctcagc gtatgtggga tgcttctccg tatgaagtca
    35401 aaatctatgc aactctggat cgggaagaag cattgaaaga tgcggatttt gttacgactc
    35461 agtttcgtgt cggtcaatta gatgcacgaa tcaaagacga gcggatccct agttattatg
    35521 ggatgattgg gcaagaaaca aatggtgctg gcgggatatt caaagctttt cgtaccgttc
    35581 cagtggtttt ggcaatcgtt gaagacatga agcgtttatg tccaaatgct tggctgatca
    35641 atttcgcgaa cccaagcggg atggtgacag aagcagttat cagatatggc aaatgggata
    35701 aagtgatagg attatgcaat gtgccagtaa tggcgatgat gacagaacca acaatgcttg
    35761 gcaagaaaaa agaagagctg atctacaagt ttgccggtct aaatcacttc cattggcaca
    35821 aagtagcaga taataaagga aatgaccgca cggcagaatt gattgatcgt ttatacgatg
    35881 aagacaatgg attgccgaaa aatatctttg atatcccgtt tttcaaagaa caattaaaac
    35941 aaatgaacat gatcccttgt ggctaccacc gctattacta ccgtgaagaa gaaatgctgg
    36001 aacatgcttt agaagaatat caaacgattg gtacaagagc gcagcaagtg aaagaaacag
    36061 aagcagagct gtttgaatta tacaaagatc cagaattgga tcataaacct gaacaattgc
    36121 aaaaacgagg tggtgcttat tattcagatg ctgcctgtga aacgattgct tcgatctatg
    36181 gaaataaaga aacacagatc gtggtatcta caaagaatga tggagctgtt cctgatctgc
    36241 cggcagattg tgtagtagaa gtcactgcct atgttggcgc acaaggcgca cgaaatgtag
    36301 cgtttggttc ccttcctgct gctgaaagag gctggttgca agtaatgaag aatatggaat
    36361 tgctgacgat cgaagcagct gtgactggtg attatggaac agctttacaa gcgtttacga
    36421 tcaatccgct tatacgatca ggaaagacag caaaacggat catgaatgag ttgtttattg
    36481 cacatcagca gtatttaccg caattcaagg aaacaatcag ccggttaaaa gcagaaggaa
    36541 tcgaagtcca agatgaactg gcaagagaat tatcaagcaa tacacatgta atggaggaag
    36601 tatgatggga ttcagaaaag attttttatg gggcggagct acagcagcca atcaatgcga
    36661 gggcggttat gatgaaggcg gacgtgggtt agcgaacgta gatgttgtgc caatcggaga
    36721 agaccgtttt cctgtcatta ctggaaaaaa gaaaatgttt gaatttgatg aaaatcattt
    36781 ttatccagca aaaattgcga tcgatatgta tcatcattat aaggaagata ttgctttatt
    36841 tgcagaaatg gggttcaaga cttatcggat gtccattgct tggagtcgta tttttccaat
    36901 gggcgatgaa aaagagccaa atgaatctgg tttgaaattt tatgaggatg tctttaaaga
    36961 atgtcaaaag tacgggatcg agccgcttgt gacgattaca cattttgatt gtccgatgca
    37021 tttgatcaaa gaatatggtg cgtggcgttc cagagaactc gttggttttt atgaaaatct
    37081 gtgccgagtc atcttcaatc gctataaagg acttgtcaaa tattggttga cattcaatga
    37141 gatcaatatg atccttcatg caccattcat gggcgcaggg ctgtattttg aagaaggaga
    37201 aaatgaagaa caaatcaaat atcaagcggc acatcatgag ttggttgcaa gtgccatcgc
    37261 aacgaagatt gctcatgaag tagatccgga gaacaaagta ggctgtatgc tggcagcagg
    37321 agcatattat ccatacactt gcaatcctga agatgtttgg gcttctagaa aatcagatcg
    37381 agaaaattat ttcttcattg atgtccagtc acgaggagaa tatcctgctt atgcattgaa
    37441 agaaatggaa cgaaatggca ttcagatcaa aatggaacct ggggatgccg cattgctgaa
    37501 agaacatact gttgatttta tctccttttc ttattattct tctcgagttt cgactactga
    37561 caacgaactc ttggaacaga ctgcaggaaa tatctttgct tctgtcaaaa atccatatct
    37621 ggaagcaagt gaatggggtt ggcaaattga tccattaggc ttgagaataa cgatgaatga
    37681 tatttatgat cgttatcaaa aaccactatt tattgtagaa aacggcttag gtgcagtcga
    37741 tacgcccgat gagaatggtt atgtggaaga tgactatcga atcgactatc tggcagcaca
    37801 tatccaggca atgaaagatg cagtagaatt agatggagtc gatctattag gctatacgac
    37861 ttggggatgt attgatttag tatcagctgg tacaggtgag atgaagaaaa ggtacggatt
    37921 catttatgtc gatcgtgaca acgaaggaaa tggcacatta gcccgttcga agaagaaatc
    37981 attcaactgg tataaaaaag taattgattc aaatggagaa aatctttcga atgattgatc
    38041 aatgatttat aaaagtctat tgtttttcag cggatttttc gagtataata aagctattcg
    38101 agaaacgcaa gcagaacaat agactttttt tcgcattggc tgtaaagtat gcttacttga
    38161 ttaagtaaag gagaatctac atatgtatga tgtaatcgtt attggcgcag gtcctgctgg
    38221 gatgactgct gctttatatg cttcacgttc aaacttatcc gtattgatga tcgaacaagg
    38281 cgcccctggc ggccaaatga acaatacggc agaaatcgaa aactatcctg gtttcgattc
    38341 aataatgggt cctgatctag cttataagat gtatgaaggt gtttcaagat ttgggacaga
    38401 aaatgcttat ggaatcgtgc aagatatcaa ggatcacggc agccacaaag aagttatttg
    38461 tgaagataaa acttacgaag gaaaaacagt cattatcgca acaggctgta tccaccgtaa
    38521 attaggcgta cctggcgagg aagaatttgc tggacgtggc gtttcttact gtgcagtttg
    38581 tgatggtgcg tttttccgta acaaacgtct agtagtgatc ggaggaggag actcagcagt
    38641 agaagaagcc atctatttga cacaatttgc ttctgaagta gtcatcgtcc accgtagaga
    38701 tgaattacgt gcacaaaaga tcatccaaga ccgtgcattt gcgaatgaaa aaatttcctt
    38761 tatgtgggat acagtcgttg acgaaatcgt tgggaatgaa atggtagtga caggtgtgaa
    38821 agcacgtaat gtcaaaaccg gcaatgagtt tgatttggca gcagacggag tattcatcta
    38881 tgttggactg gatccattaa ctgcaccatt tagagaagca gggttgacga acgaagctgg
    38941 ctggattcca acagacaacg aaatgaagac gaatatacca ggcgtttttg ccatcggaga
    39001 tgttcgtgaa aaagatttgc gacaaatcac tactgctgtt ggtgatggtg ggatcgctgg
    39061 acaacaagtg tataaatact tggaatcttt agaagaaaca actgcataag tttaaaaaga
    39121 gtggaagaac ggtttaattg ttcttttact cttttttttc gcgtgaaatc tactgaatcc
    39181 gatttatgcg aatacttgca accgtgcttg ataaaagaat cctttcttga tttaagcgtt
    39241 ttttggtcct catactttca tatctaataa gtgaatgcta tacttattaa gaaccatatt
    39301 tcagaaagag gtgttatttt catgtcttgg gaacaagtgt atgaacaatg ggtacatgaa
    39361 gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc tgggtgaaga tcctgaaaaa
    39421 cttgaagatg ctttttatgc accgttggaa ttcggtactg caggcatgcg gggaatctta
    39481 gggcctggga tcaatcgaat gaacatctat acggtaagac aagcaacaga aggacttgca
    39541 cgtttcatgg atacacaagg cgaagaaaca aaacgccgag gtgtcgctat cgcttatgac
    39601 tcacgccatc aatcaccaga atttgccatg gaagcagcca aaacattagc acatcacaat
    39661 atcccatctt atgtattcga aagtctacgt ccaacaccag agctttcttt tgcagtccgt
    39721 catttgaaga cgtttgcggg aatcatggta accgcatccc acaatccagc agcttacaac
    39781 ggatataaag tctacggaga agatggggga caaatgcctc ctgcagacgc agatgcattg
    39841 acgaaatatg tccgtgaagt cagcaatccg ttaaaagttg aagtattatc tgacgaagaa
    39901 gctgaacata gcggattgat cacgatcatc ggagaagaag tggatgccgc ttatctagaa
    39961 gaaatcaaag cagtaacgat tgatcatgaa ttggtggaaa caatggggaa agatttgaaa
    40021 cttgtgtaca caccacttca cggaacagga aaaatgttgg gcgaacgtgc gttgaaacaa
    40081 gctggattcg aacaattcgt actagttcca gaacaagctg tcgcagaccc agatttcact
    40141 acagtcaaat caccaaatcc agaagaacac tctgcttttg agtatgcgat acgacttggc
    40201 gagaaagaag gcgctgattt gctgattgca acagatcctg atgcagaccg tttaggtgca
    40261 gccgttcgtt tgccaaatgg ctcctatgaa gtattgaccg gaaaccaaat cggagcactg
    40321 tttgctcaat atattttgga agcacaccaa tcgcgaggca ctttacctga aaacgcagcg
    40381 gtattgaaat cgatcgtttc tagcgaatta cctacagcaa ttgcggaaca ctatggggct
    40441 accatgttca atgtattgac aggattcaaa ttcattgctg aaaaaattca acaatttgaa
    40501 gaagatcatt cccatacttt tatgttcgga ttcgaagaaa gttacggtta tttagttaaa
    40561 cctttcgttc gcgacaaaga cgcgatccaa gcattgctct tattagcaga agtagcagct
    40621 tactataaaa aacaaggcaa aacgttgtat gatggcttgc aaacgatttt tgaaacctat
    40681 ggctactacg cagaaaaaac gatttctgtg acaatgagcg gacaagaagg ttctgcgaaa
    40741 atctctgctt tgatggaaaa attccgtgaa gaagcaccaa cagaatttgc cggagcaaaa
    40801 gtcatccaaa cagaagactt caaagagttg acgagaacag accgtgaagg aaacgtagaa
    40861 aaactaacga caccgccatc tgacgtattg aagtatacgc tggaagaaaa tggctggatc
    40921 gctatccgtc cttcaggaac tgagccgaaa atcaaatttt atatcggtgt aaaaggaaac
    40981 tcttcagaag acgcagctga aaaagtagcc gcttatgaaa cgattatcca aaagatgaca
    41041 gaagaataaa aaaacttgag actgatggaa aaagtggtaa actttagaag tcagtctttt
    41101 ttcaagttgg aggtgaatgg attgccagac acgcaaaaaa caaaagtaga gaaagtcagc
    41161 caactattca aaatgttgag tgaccccact cgtctcaaaa ttctgctgta tctgaaagac
    41221 ggggaacaaa atgtgacagc aatcatccaa gcagtggaaa tggaacaatc tgctgtatcg
    41281 catcaactgc gtttattaag agaaaaccat gtcgtcaagt cccatcgtga gggaaaagcg
    41341 attttgtata gtcttgatga ttctcacgta ctggatattt tgaaccagac actcaaacat
    41401 gtggaacatc aataacgata ggtgcaaata gagacaaact ggactgtgtc aacgaatctt
    41461 cttcgctgac acagtccagt ttttttaaag aaaaattgtt agtctgtttc ttcggtggtt
    41521 tcagttccat aataactatg gtcttctagg tctaaagcat tcaaaataag cgaagctgtt
    41581 tcttcaatag ataaagaagc gacattgatt actacgcagc ccaatttttc ataaagatca
    41641 ttcgcaaaat cgagttcagc tcgtattttt tcgatatcgg aataagcagt atcaggattt
    41701 aatccgtaag agcgcatccg ttctttacgg atattgttta aaatatctgg gttattcgtc
    41761 agtcccacga tttttttagg atcgatctcc caaagctgtt tgggaatatg tgcttgtggg
    41821 atcaaaggta gattggcaac ttttaaattt ttgttagcta aaaacaagct caaaggcgtt
    41881 ttggaagttc gggatacgcc tagcaaaacg acatctgctt ctagaaaccc acgaggatct
    41941 ttcccgtcat cgtattttac agcaaattcc attgctttga tccgtttgaa atagttctca
    42001 ttcaataaat gaagggcgcc aggttgccga atgggcttga taccagttaa gcgctcgatt
    42061 tctgatatcg gcggagtcat cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa
    42121 cgcgtcatct cgatcaattc ttgattgatc aatgtgtgca ggacaagagc tttttcttct
    42181 agtgcatctt gcagtgcctg catcaatgtt ttctcatctg tcacaaatgt tctgcggaat
    42241 aaattgaact caacgctagg atattgggcc atagtggctt gcgctagttt tgtcgcagtt
    42301 tctccagcag agtcggagat gacaaaaaaa gtaacgattt ctgtagaatc ggaagacatg
    42361 gataaaacct cacttgcatt ttttttatgt ttattatacc atactctaga atgtgtactt
    42421 cgtaatcatt ttgatttgga ggaataagtc atgaaaaaac agacgttgat cgaacaatca
    42481 ctagaaacat tgaaaaataa cggttttaaa tatactaaaa aaagagaagc attgttaact
    42541 tatttagtga agcgcaatcg ctacgtatcc gctaaagaag tttttgacta tatgaatcag
    42601 gaattcaaag gagtaagtta cgatacgatt tatcgaaatc tccatgattt tgcagaaatc
    42661 gatttactgg aagaaaccga tttaaatgga gagatgaagt ttcgttttca ttgctgtcaa
    42721 ggggaaatcg gccatcacca tcactttatc tgtacggtgt gcggaacaac gaaagaaatc
    42781 catatgtgtc caatgaatta tttcgaagaa cagctcggtg gatgtacgat cgaagggcat
    42841 agattcgaga ttttcggaag atgtgaaaat tgtcaataag gatttaaata aaataacatt
    42901 tgataatttt aaaaaaaagc aagatttcct tgacggtcaa aacagcatta gatataatat
    42961 ttacaggaca gtcttgtttt ttgagatata tttcaataac aattacggtt actttttaag
    43021 ctcggaggga gggaattaac atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg
    43081 atgctcttcg tcgcttcaaa cgttccgtgt caaaagcggg tactttacaa gaatctcgta
    43141 aacgtgaatt ctacgaaaaa ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa
    43201 aacgcaaaaa attctagttc tgtgatgaat tggagttggt agtgtgtcac tactaagtag
    43261 attaaacgat gacatgaaaa cagcgatgaa agcgaaagat aaggaatctt tacaagtcat
    43321 ccgaatgatc aagtcatcta tacaaaacga gcagattaaa gaaggtcacg acttgacaga
    43381 agaagaagaa ttaacggtgt tgtctcgcga aatgaaacag cgacgtgatt ctttgcatga
    43441 gtttgaagaa gcaggacgtg acgatttagc tgaaaaagta aaaagtgaaa ttgtgatagt
    43501 cgaaaaatac atgccggaac aactttccga cgaagaaatt cgacaacttg ttcaagaagc
    43561 aatcacgcaa actggtgctt catcaatgaa agaatttggc aaagtcatgg gtgcgatcat
    43621 gcctaaagtc aaaggaaaag cagatggaaa ccaagtaaat gccattgtga aagaactatt
    43681 acaatcttaa ttttatcatt taaactgcgg acaatcattg tccgcagttt ttttgtgctc
    43741 gtaaaaaaga gatctaaaaa atagctagac gaaagaatag gattctttca tctagctgtt
    43801 tttttcctgt ttaatatttc ttatgttaaa ttgtgtttga tctggttatt cttctggtag
    43861 ttcatttgct tcttgtttat accctgatag gcgataggcg taatagaaga gtaagctgcc
    43921 agcataccca agtaacgttg ggaccatcca gccaagtccc aatgcagcaa aaggaacgtg
    43981 atgatgataa aattcgagca tgcttgctac aactctttgt tgactcacta aagcaggcga
    44041 atttgccagc atatccaata cggcgggaac agcagcaaac aacatcgtta cttgatagac
    44101 aaatggtgtc ttttggaaaa attttgctgt taaggaaagc aaaatcaacg caagcgacaa
    44161 tggatacaaa agcatcaata ctggtaaaga ccattggatg atattgtcca aaccagcatt
    44221 tgcaacgaca aaagagacga aagtcgttaa ccgcagccat gtcatataac tgactttagg
    44281 gaacagttta tggaaatctt gtgcaaaaga aacaacgagt cccatagcgg tagtgaagac
    44341 acctaatgtg accatcactc ctaagaagct agcgccgaag ttacctaaat aaaaattagt
    44401 gatctgagaa agtgcagttc cgccattttc tgataaagac atcttattca aactcatcgc
    44461 accgagaaga acaagaccaa aatagatcat gacttctagc gcaacgctca ggctaccagc
    44521 ttttgctgtt aattctgtga cttttttatc tttgaaacca aggtcacgga ctgcatggac
    44581 aaaagtgaca cttaaggcaa gcaaggctac tgcatcaacc gtgttataac cttctaggac
    44641 accatttgtt aaagcgtttg tttggtaagt atctgcagca gattgattta gatttcccat
    44701 tgggtggaat aaagaaacga caaaaatgat tgctaataat gcaagaaaca aggagtttag
    44761 atactttccg acgtatttga ccaaccgtgt gggatggaca gtaaggaaat aagcaagtaa
    44821 aaagaaaaga gcagagaata aaaacatacc taatgaatta tatttttcag gcaaaaatgg
    44881 tttagcagcc atttcaaaag cagtagcagc tgtccgtggt gtaccgaaaa atggtccgat
    44941 cgtcaaatga acaaggatta agaacaaggc agcataccat tttccgacag gtttagctaa
    45001 gtcatacaga ccatcacttt ttgtcactac tacagctaag atagctagta atgggaaaag
    45061 agcgcctgag atggcaaatc ctacgccagc actgacccag tttgcgccag ccatttgacc
    45121 taagtggaca ggaaagatta gattacctga cccgaaaaac atcccgaaga taagtgaact
    45181 gaccatcaat agttgtttcc atgaataagt ttttgtttct tccataaatt tttcctccaa
    45241 taattatcat tttgattgac gatataaacg ttgcagcaat tagtaatgcc actaatatca
    45301 gattccatga aaaccaccac cttaaaatat aaaaaaaagc ccttttccac ttgggaaaag
    45361 ggctctagat tctagaacgg taccaccttt tttcgcttaa aaaagcttct tcattcacgg
    45421 acgctttttg ataaagttat ccgctagcct gataatggag gcagccacaa tgatctacgt
    45481 tttcgacatt gactcggaaa tgatgttcgt ctattcgatt tattgtcatt tctcacttac
    45541 atgactcact gggaataaat cgttgaatag cctactgttt tcctcgacgc tttgctgtat
    45601 gtttatttgt tgacgtaata atacacctgt tttttttgaa atgcaagaat attttaacta
    45661 ttttgttttc tttttttgaa acaagaaaaa tacctagaga atacctctga atgaaacgga
    45721 atattttgtt ctccactttc atataactta cattctcaga ttctttttat caaaacgaga
    45781 cctttagcga atggaaacag ctatttttta acgtttcttg gtacgtatgg ttaaacgact
    45841 cttttacaac cttttcttat ttgctatata gtttcttctg agtatggtaa aataggaaag
    45901 aatgaatcga aaaaaagggg atgtctattt ttgacggaag agtctagttc tttagagatt
    45961 agattgacag ctgctgatga tgtcagcatg cttttaggtg cacacgacaa gcatataaaa
    46021 ctgatcgagg aaacaacgga aacaaccatc catacacgtg gagaaatcat tcagatcatt
    46081 ggtgaacaat cccagatttc cttggctgcc tcagtcattc ggacattaca ggaattgatc
    46141 aaacgaggaa tccacgtaag tacaccagat gtcgtgacgg cattgaaaat ggggcaaaaa
    46201 ggtacattag attattttgt tgagatgtat gaggaagaga tcgtcaaaga ccgtaatgga
    46261 aaacctatac gggtaaaaaa cagcggtcaa aagaaatacg ttgaatcggt agccaaacat
    46321 gacatcgtat ttggtgtagg gccagccgga acagggaaaa cgtttcttgc cgtagtatta
    46381 gcaattgctg ccctaaaaaa aggcgaagtg caaaaaatca ttttgacgcg gcccgctgta
    46441 gaggcagggg aaaacctagg ctttctaccc ggggatctga aagaaaaagt ggatccttat
    46501 ctgcggcctg tttatgatgc tttataccaa atttttggat tggatcatac gaaccgattg
    46561 atggaacgag gcgtaatcga aatcgcacca ttagcttaca tgcgggggcg gacattggat
    46621 gatgcattcg ttatcttgga cgaagcacaa aacacgacag tcgctcagat gaaaatgttc
    46681 ttgacccgtc tcggttatca atcaaaaatg attgtcaatg gggatacaag tcagatagac
    46741 ttgcctcgag ggacgacaag tggtcttgtt catgcagaac gaacactaaa gcaaatcaaa
    46801 aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc ggcaccctgt agttgccgaa
    46861 atcatcaaag cttatgaaaa ggcggatctt catcaagaat aagctttgct taggaggata
    46921 agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa actcggaaag gcagcattgc
    46981 ctttccttct gttcttgttt tttatcattg tctgcggcgt tacatttagt agtgtcaaac
    47041 aaaaggccaa tgatttcaga gaaggccaag tagcagaaga gagtatccgt gcgaataaaa
    47101 cgatcgagaa cacggaagaa accgaacaaa gaagaaaact ggcagcagaa gcagtgactc
    47161 cagaatatac ttatcaaaaa gatctggcag atgaccagaa caatcggatc aagcaattat
    47221 tcgaattgat cgataagacg aatgatacca tcaacaaatc ttataatgac aaggtagata
    47281 aggcgaagga agatgaaaat atcccgaaac ctactacaga agagcggatt gctacactga
    47341 agaaaaattt tgaaaacgtg aatgcagaaa acgttgcgtt ttaccaaaaa ctgccgaaca
    47401 atttttattc gacgatattt gaaatgactg aaacggaaat tcacacggtt agggatgaaa
    47461 gtttgaagct cattgacgaa cagatgagta aacaagttcg tgaaagcgaa ttggaagctt
    47521 ttaaacaaga agcagaagat cagattcagt atttgaatgt cacaacagag cagcaacaaa
    47581 tgattcgcta cttagtggat caaggcattg tagtcaatga tgtattgaat gagaaaaaga
    47641 cagaagaatt aaaacaatct gcacgagaag ctgtacaacc agtgatgatc ttccaaggag
    47701 aaatcattgt tcgcgaagga aatcaaattg atgcttcagc gatgaaaaaa ttggaattac
    47761 taggattgac gaatcaaacg acttcgattt tcccactcat tgccatggtt ttagctgttt
    47821 tgcttcaaat cggcgtactg atttataatt cacttcaatt ccaaaatgca ggagaaagaa
    47881 caaaatacgt gcttttctat gtgacagcta tgtcagtcag catagtgctg atgaaatttt
    47941 tccaattatt ccaaacggaa caagctactt atatcccgct gttttatccc gctgcttttg
    48001 caccacttgt tttaagcttt ttcttgaacc gaagagcagg gatcatggct ggtattttcc
    48061 aagcagtatc tgcacttttc attttttatg attcgattgg cacgaacttt ctaacgatta
    48121 tcttgatgtc ttatgttttc tctgggctca tggcaacgat tgtcagacgc aaacggatct
    48181 cagaacaagg attatctgct gccatgtggg tcattatttt tcctgtgatg atggatgtca
    48241 ttttgatcgt ctatcaagga atgagctttg gtgatgcaac gacttggtta atgcttgtct
    48301 gcgggttcac tggtgcactc ttctcattct tgctgacgat cggtctacac ccatatattg
    48361 aattgatggt caatgatgac agtgtaattg tattaaatga actcagcaat ccaaatcatc
    48421 ctttattgaa acagttgctg gaagaagcgc caggtactta tcatcatagt atgatggtag
    48481 ctaatctaag tgctaacgct gtagcagaaa tcggcggacg ttctttgcta actcgagtag
    48541 cttgctatta tcatgatatc ggcaagatca aacatgctag ctttttcgtt gagaacttgc
    48601 cttcaggagc ggaaaacccg cacaatttct tattgccaga agacagtaaa catattatct
    48661 ttggacatgt gacagatgga gccaaaatat tggaagatta taatatgccg caaatggtga
    48721 tcgatatttg caaacagcat catggaacca cgctaatgcg ttatttctac atcaaagcga
    48781 aagaacgcaa tccggaagta acagaagagc aattccgtta tcctggacca aaaccgcaaa
    48841 gtcgtgaagc agggatcgtc aatattgctg acagttgtga ggctgcagta cgggcaatgg
    48901 atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa tcttatcgaa gagcgtattt
    48961 ccgatggtca attagacgat agtggactga ccttgaaaga aatccgaaaa gtggaaaaat
    49021 cattgatcag cggtttaagt agcaccttcc attctcggat caaatatcct aaaatgaaaa
    49081 cagaagttga gaagataaag gaagaacaag aagaatagaa agaagcgatg tataatggat
    49141 attaccttta tggatgaaac aaaccaagtc tcagaagaga aaatgaaaga aatcgatgac
    49201 ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg aaacagaaat gtctgttact
    49261 ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt accgtggaaa agatgcaccg
    49321 acagacgtca tcagttttgc tctggaagaa gaaggcgaag acgaaatccc agtgattttt
    49381 gaagatggag agaatccttt gcctcgtgta ctaggagaca tcatgatttc tattgaacgt
    49441 gctgctgaac aagcagaagc ttacggccat tcatatgatc gggaacttgg ctttttagcg
    49501 gtacatggat tcttgcatat caacgggtat gaccatatga caccagaaga tgagaaagaa
    49561 atgtttggtt tacagaaaga gatcttggat gcctatggac tcaaaagata aaaacgtaga
    49621 gaaaaacaaa catttcctga tgtctgtgga atttgctgtc caaggagtca aaaccgtttt
    49681 tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt atccttgctt tatttatggg
    49741 cgtgattttc cagcttgatc ggatagagtg gttatggctg cttttagctg tttttctcgt
    49801 ttggatagta gagatcatca atactgtttt tgaaaatgta gtggacatgt ttacggattt
    49861 tcattttcat ccgattggga aaaaaatcaa agatatggca gctggagcag ttttagtgac
    49921 cgcttgtttt gctgtcatca ttgggttgat tttatttgtc cctaaaatat atcatttgtt
    49981 gtttaattaa aaaggagaac gtatatgcca gaacataaat cagggtttgt cgcaatcgtc
    50041 ggccgcccga atgtaggaaa atccacatta ttgaaccgaa tcgtgggtca aaaaattgcc
    50101 attatgagtg acaaggcaca gacaactaga aataaaatcc aaggagtata tactacacca
    50161 gaagctcagc tgatttttat cgatacacca ggaatccata aaccaaagca tcgtctagga
    50221 gattttatgg tagaagcagc ttacagtgcg ttgaaagaag tggatgcagt cttatttatg
    50281 atcagtgctg atcaaaaacg cggccgggga gacgatttta ttattgaacg tctaaaaaac
    50341 gtgcattctc cagtttatct gattatcaat aaaatcgata agatccatcc agacgagttg
    50401 ctgggaatca tcgaagatta ttcgacgcag atgccttttg cacaagtgat tccgatctct
    50461 gctacagaag gaaacaatgt cgaacgctta atggatgtat tagtcagtga aatgccagag
    50521 ggcccacaat attttccaga tgatcaagtg actgatcatc ctgaatactt tatcgtttct
    50581 gaattgatcc gtgagaaagt cttgttttta acacgagacg aagtgcctca ttctgtcgca
    50641 gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa tccatatcca ggccacgatc
    50701 atcgtcgaaa gagatagcca aaaaggaatc attatcggca aaggcggaaa aatgctgaag
    50761 caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt taggagacaa agtatttcta
    50821 gaactttggg tcaaagtcca aaaagactgg cgagacaaaa aagtctatct gcaagacttt
    50881 ggctaccgaa aagacgaata ctgatattct gaacttctta taggttaagt atagaaataa
    50941 actggataaa aatccgcacg ttgagaaaaa cggcggaaga tcgaaacata aataatctgg
    51001 atcggcagaa tctgcttttc cagatttatt ttttaggagg gagaatatga gccagcttgc
    51061 agaatcgaaa ggcttgattt tatctagccg taattacaaa gaaaaagata aattagtcaa
    51121 aatctttacg gaatcttctg gcaaaatgat gttttatatc aaaggaatcc atcggcaaaa
    51181 tcagccatta gctccggctt tgctcccctt taccgaagca gtctatatcg gtaattttcg
    51241 aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc catgcgtttc ggaacatcca
    51301 agaagatatc tttttgagcg gttatggtac ctatttgcta aatctagtag atgcagcaat
    51361 cgaagatcat caatatgacc cgcatttatt ccaatttacg caacaagctc tgcagcggat
    51421 ggatcaagga gatgatgcag aaatcatcac gaatatcttt gaagttcaaa tactgcaacg
    51481 cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc ggggaaacaa aaggaaaatt
    51541 tgactattct tccaaatctg gcggtgtgat atgcgagaaa cactggccaa tggacgaaca
    51601 ccgttatcat gcagatcctc gcgctgttta tttcgttcga atgttttcgg ctatttctta
    51661 tgataagatc agtggaatcc acttgaaaga agaaacgaag caggcaatcc gtgacctcat
    51721 tgaccagttg tatgatgaat atgtcggtat tcatctaaaa agcaaaaagt tcattgatca
    51781 aatgaagtct tgggcacacg tattaaagac gccagagaaa aatatgcctg aaaaagagat
    51841 acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa tcaaaactat ttgacaaata
    51901 cagggacttc cgtctattat taaccgtaga ttaaatagat cgttcactga gaggatctat
    51961 tatataaaca agaaaactgc aaaatgaata cgtacattga ccaagatcag taaaaaacac
    52021 gtggtttagc gagttcggga gagtggaagc cggacccaaa cgatttttga agaggcactt
    52081 gcgagtacga taaatgaagc tgccgaatct ttattcggaa gtagggtgga accgcgaaat
    52141 tattcgtccc tatgtctgta atcagacata gggacttttt gtcgttttct ttcctgtatt
    52201 cttattgaca gcaacgctta aaaattgaaa ggggcaaaac agatgagtaa aaaattgacc
    52261 gtccaagata tgattttaac attacaaaaa ttttggtcat caaacggctg catgctgatg
    52321 caagcttatg acacagaaaa aggagcaggt acgatgagcc catatacatt cttacgggca
    52381 atcggaccgg aaccatggaa tgctgcttat gtagagccat cccgccgacc agctgacggg
    52441 cgttatggag aaaacccaaa ccgtttgtat cagcatcatc aatttcaagt cgtaatgaaa
    52501 ccttcacctg aaaatatcca agagctttat ttagaaagct tgcgtctttt aggaattgat
    52561 ccattagaac acgatattcg ttttgttgaa gacaactggg agaacccatc gatggggtgt
    52621 gccggtgtgg gttgggaagt atggcttgat gggatggaga tcacacaatt cacgtacttc
    52681 cagcaagtcg gaggactacc ttgtaaacca gttacttctg agatcactta tggactagaa
    52741 cgtcttgctt catatattca agaagttgaa agtgtctatg atctggaatg ggctgatggt
    52801 gtgaaatatg gcgagatctt caaacaacca gaatatgaac attccaaata ttcttttgaa
    52861 gtaagtgacc aagacttgct actaagcaac tttgaccgtt ttgaaaaaga agctaaacgc
    52921 tgtatcgatg aaaatcttgt tcatccagcg tatgattata ttttgaaatg cagccataca
    52981 ttcaatttat tagatgctcg cggtgcagta tccgtaacag aacgagcagg ttatctttcc
    53041 cgtatccgca acatggctcg tgctgtagcc aaaatctttg tggcagaacg cgaaaaatta
    53101 ggattccctt tattgaataa aaacacagca aatccagaaa acatgaagga ggctgaataa
    53161 gatggcaaaa gacttattac ttgaaatcgg gctggaagaa atgcctgctc atgttgtcac
    53221 accaagcatg aaacaattag aagaaaaaac agcaaaattt ttagatgaaa accaattgac
    53281 ttatgatacg atcgaaacgt tttctacacc gcgccgctta gcaatcaaag taacaaacat
    53341 tccagaacgt caagcagaca gcgaagaaga agtcaaagga ccagccaaaa aaattgcttt
    53401 ggacgcagat ggaaactgga cgaaagcagc tgaaggattt gtccgcggac aaggtctgac
    53461 gacagatgct atcactttta gagaattaaa aggcgtggaa tatgtctatg taacgaaata
    53521 cactcacggc aaagaagcaa aagaagtatt gacaggttta gcagatgtca ttaaaagcct
    53581 cactttccca gtaacgatgc actgggcaga atacgatttt gaatatattc gccctattca
    53641 ctggatcgtg gcattgttag atgacgaaat aatcccgttt gagatcttgg atgtcaaaac
    53701 agacagagct tcaagaggtc atcgtttctt aggcgaagat gtgacattta cgcatgcaaa
    53761 agaatacgaa gaaaaattga atgaacaatt tgttattgct tcaccgcaaa aaagaaaaga
    53821 aatgatcatc acacaagcaa acgaattagc cgaaaaaaat cactggatca ttgatctaga
    53881 tgaagattta ctagaagaag tcaacaattt agtcgaatat ccaactgtct tttctggcga
    53941 cttcgaagaa aaatatctat ctgttcctga agaagtacta gtcacttcta tgaaagaaca
    54001 tcaacgttat ttcgaagtac ggaacgatca aggtatgttg cttcctcatt ttatttctgc
    54061 tcgtaatggc gatcgggtaa aattggaaaa tgtggtacga ggcaatcaaa aagtattgac
    54121 ggctcgttta gaagatgcgg aattctttta taatgaagac aagaaagctt caatcgacca
    54181 atacgttgaa aaattaaaaa ctgttacttt ccatgaaaaa atcggtagca tctatgagaa
    54241 aatgcaacgg gtacaagtca tcgctcgcat cataggcgaa caagtaggac tatctgagga
    54301 agaattgatg gatctgcaac gcgctgctga aatctataaa tttgatctag taaccaacat
    54361 ggtaggagaa ttccctgaac tacaagggat catgggagaa aaatatgctc tgctgaaagg
    54421 cgagaaacca gccgtagcaa cagcgatccg tgaacattac atgcctatat ccagtgaagg
    54481 agatttacct gagactacag taggttctgt tttagcgatt gctgataaat tagatagtat
    54541 cttttctttc tttgcagttg ggatgatccc aagtggctca aacgatccat atgctttgcg
    54601 ccgtcaagca tatggtgtcg ttcgaatcat cgaatcgaaa aaatgggcct ttcctttgtc
    54661 tgttctgcaa gaaacgatta gtgaagttat cagtaaagat acggatcgtt ttggcattgg
    54721 cttgtcagcg ggacaacaac aggtgatcga ctttatcaaa ggccgtctgc gccaattatt
    54781 gacaacgaag aatatccgtc atgatgtcat cgaagctgtt ttgaatgcag aacaaaaaga
    54841 tctgacaaaa gtatttgcag cagcacaatt gttcaagcag catcttgcag atgaagactt
    54901 caaaccatcg atggaagctt tgacacgtgt cgtgaactta gcgaaaaaag cggagcttga
    54961 aaaacaatca gaagtagacc ctgaactgtt cgaaaacgaa gcggagaaag aactgcacaa
    55021 agcagtagag gcaatgaaaa caagctttgc tgaccaaacg atcaacgaaa attatgatca
    55081 gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat cagacgatgg tcatggcaga
    55141 agaaccagca gtacgtaaga accgattaac gcaattaagt caaattgctg ccatggcttt
    55201 gtcactagca agtttagatc aaatcatcac aaaataattt ctaatatgta aataaaccag
    55261 agaac
//
LOCUS       DACCXM010000006        53884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCXM010000006 DACCXM010000000
VERSION     DACCXM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.006"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(34..261)
                     /locus_tag="IVY61_000283"
     CDS             complement(34..261)
                     /locus_tag="IVY61_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7969424.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(258..776)
                     /gene="msrA"
                     /locus_tag="IVY61_000284"
     CDS             complement(258..776)
                     /gene="msrA"
                     /locus_tag="IVY61_000284"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7969425.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(796..1509)
                     /locus_tag="IVY61_000285"
     CDS             complement(796..1509)
                     /locus_tag="IVY61_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7969426.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1512..2408)
                     /locus_tag="IVY61_000286"
     CDS             complement(1512..2408)
                     /locus_tag="IVY61_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7969427.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2566..3408)
                     /locus_tag="IVY61_000287"
     CDS             complement(2566..3408)
                     /locus_tag="IVY61_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7969428.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3540..4193
                     /locus_tag="IVY61_000288"
     CDS             3540..4193
                     /locus_tag="IVY61_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7969429.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4256..4783)
                     /locus_tag="IVY61_000289"
     CDS             complement(4256..4783)
                     /locus_tag="IVY61_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7969430.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4803..5750)
                     /locus_tag="IVY61_000290"
     CDS             complement(4803..5750)
                     /locus_tag="IVY61_000290"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7969431.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5762..7648)
                     /locus_tag="IVY61_000291"
     CDS             complement(5762..7648)
                     /locus_tag="IVY61_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7969432.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(7808..9886)
                     /locus_tag="IVY61_000292"
     CDS             complement(7808..9886)
                     /locus_tag="IVY61_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7969433.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(9899..10174)
                     /locus_tag="IVY61_000293"
     CDS             complement(9899..10174)
                     /locus_tag="IVY61_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969434.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(10536..10991)
                     /locus_tag="IVY61_000294"
     CDS             complement(10536..10991)
                     /locus_tag="IVY61_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969435.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(11145..12353)
                     /locus_tag="IVY61_000295"
     CDS             complement(11145..12353)
                     /locus_tag="IVY61_000295"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7969436.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(12465..12797)
                     /locus_tag="IVY61_000296"
     CDS             complement(12465..12797)
                     /locus_tag="IVY61_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7969437.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            12945..13820
                     /locus_tag="IVY61_000297"
     CDS             12945..13820
                     /locus_tag="IVY61_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7969438.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(13902..14483)
                     /locus_tag="IVY61_000298"
     CDS             complement(13902..14483)
                     /locus_tag="IVY61_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7969439.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(14489..15748)
                     /locus_tag="IVY61_000299"
     CDS             complement(14489..15748)
                     /locus_tag="IVY61_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7969440.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(15750..16796)
                     /locus_tag="IVY61_000300"
     CDS             complement(15750..16796)
                     /locus_tag="IVY61_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969441.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(17031..17306)
                     /locus_tag="IVY61_000301"
     CDS             complement(17031..17306)
                     /locus_tag="IVY61_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP7969442.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(17549..18859)
                     /locus_tag="IVY61_000302"
     CDS             complement(17549..18859)
                     /locus_tag="IVY61_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP7969443.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(19041..20270)
                     /gene="rpsA"
                     /locus_tag="IVY61_000303"
     CDS             complement(19041..20270)
                     /gene="rpsA"
                     /locus_tag="IVY61_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP7969444.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(20405..21085)
                     /locus_tag="IVY61_000304"
     CDS             complement(20405..21085)
                     /locus_tag="IVY61_000304"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP7969445.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(21134..21769)
                     /locus_tag="IVY61_000305"
     CDS             complement(21134..21769)
                     /locus_tag="IVY61_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7969446.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(21835..23238)
                     /locus_tag="IVY61_000306"
     CDS             complement(21835..23238)
                     /locus_tag="IVY61_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP7969447.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(23225..24256)
                     /locus_tag="IVY61_000307"
     CDS             complement(23225..24256)
                     /locus_tag="IVY61_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969448.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            24287..24508
                     /locus_tag="IVY61_000308"
     CDS             24287..24508
                     /locus_tag="IVY61_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP7969449.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(24673..25410)
                     /locus_tag="IVY61_000309"
     CDS             complement(24673..25410)
                     /locus_tag="IVY61_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP7969450.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(25563..26903)
                     /locus_tag="IVY61_000310"
     CDS             complement(25563..26903)
                     /locus_tag="IVY61_000310"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP7969451.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(27140..28663)
                     /locus_tag="IVY61_000311"
     CDS             complement(27140..28663)
                     /locus_tag="IVY61_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP7969452.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(29041..29652)
                     /locus_tag="IVY61_000312"
     CDS             complement(29041..29652)
                     /locus_tag="IVY61_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7969453.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(29775..29900)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(29982..30698)
                     /locus_tag="IVY61_000313"
     CDS             complement(29982..30698)
                     /locus_tag="IVY61_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7969454.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(30704..31273)
                     /gene="scpB"
                     /locus_tag="IVY61_000314"
     CDS             complement(30704..31273)
                     /gene="scpB"
                     /locus_tag="IVY61_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP7969455.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(31257..32054)
                     /locus_tag="IVY61_000315"
     CDS             complement(31257..32054)
                     /locus_tag="IVY61_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP7969456.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(32024..32395)
                     /locus_tag="IVY61_000316"
     CDS             complement(32024..32395)
                     /locus_tag="IVY61_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7969457.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(32414..33301)
                     /gene="xerD"
                     /locus_tag="IVY61_000317"
     CDS             complement(32414..33301)
                     /gene="xerD"
                     /locus_tag="IVY61_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP7969458.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(33397..33870)
                     /locus_tag="IVY61_000318"
     CDS             complement(33397..33870)
                     /locus_tag="IVY61_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7969459.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(33975..34838)
                     /locus_tag="IVY61_000319"
     CDS             complement(33975..34838)
                     /locus_tag="IVY61_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7969460.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(35034..37133)
                     /locus_tag="IVY61_000320"
     CDS             complement(35034..37133)
                     /locus_tag="IVY61_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7969461.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(37353..38462)
                     /gene="rpoD"
                     /locus_tag="IVY61_000321"
     CDS             complement(37353..38462)
                     /gene="rpoD"
                     /locus_tag="IVY61_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP7969462.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(38486..40327)
                     /locus_tag="IVY61_000322"
     CDS             complement(38486..40327)
                     /locus_tag="IVY61_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP7969463.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(40509..42902)
                     /locus_tag="IVY61_000323"
     CDS             complement(40509..42902)
                     /locus_tag="IVY61_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7969464.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(43009..43869)
                     /locus_tag="IVY61_000324"
     CDS             complement(43009..43869)
                     /locus_tag="IVY61_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP7969465.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(43928..44449)
                     /locus_tag="IVY61_000325"
     CDS             complement(43928..44449)
                     /locus_tag="IVY61_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP7969466.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            44769..45488
                     /locus_tag="IVY61_000326"
     CDS             44769..45488
                     /locus_tag="IVY61_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7969467.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            45514..48105
                     /gene="mprF"
                     /locus_tag="IVY61_000327"
     CDS             45514..48105
                     /gene="mprF"
                     /locus_tag="IVY61_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7969468.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(48293..49480)
                     /gene="tuf"
                     /locus_tag="IVY61_000328"
     CDS             complement(48293..49480)
                     /gene="tuf"
                     /locus_tag="IVY61_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7969469.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            49848..50204
                     /locus_tag="IVY61_000329"
     CDS             49848..50204
                     /locus_tag="IVY61_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7969470.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(50445..51449)
                     /locus_tag="IVY61_000330"
     CDS             complement(50445..51449)
                     /locus_tag="IVY61_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP7969471.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            51615..52466
                     /locus_tag="IVY61_000331"
     CDS             51615..52466
                     /locus_tag="IVY61_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7969472.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            52483..52728
                     /locus_tag="IVY61_000332"
     CDS             52483..52728
                     /locus_tag="IVY61_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969473.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            52915..53619
                     /locus_tag="IVY61_000333"
     CDS             52915..53619
                     /locus_tag="IVY61_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969474.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        15119 a        11269 c         9251 g        18245 t
ORIGIN      
        1 aacttttttg atcataaagt acaaaactat ctcttatatg tcatgattat tttctaaata
       61 tttttcccat gcttcgtcaa agatatccat ggacggcaaa tagtcgacat tcagttccag
      121 ataattagaa atttcttcgt agctagagct gtgcttagga aattggatgt ctttaccggc
      181 ttctgtagcg aaaacagata aagcatcatg tgtgccaccg cctcgcagtg tcattaaata
      241 atgaaaaaag cttcgattca cccttcgttc ctccaatttt cttcgataaa ggcagcccgg
      301 cgtgcatggt ttcttttaaa attttcagga tgctttttat aatactcttg atggtcttct
      361 tctgctagat aaaatggttg ggctggttcg atttttgtaa cgattggctg ttcgaaacgt
      421 ccactttcag ctaactgttt cttacttttt tcagcaatga tccgctgttc ttctgtcaaa
      481 taaaagatca ccgggcgata attgtcaccg cgatcctcga attgtccgaa agcgtcagtt
      541 ggatcagttt gctgccagta aagttctact aattgttcat atgaaatcaa tgttggatca
      601 tagataatct ccactgcttc tgtatgtcca gtcgtttggc tttttacttg ttcataagtc
      661 ggatctggta catgccctcc agtatatcct gacatcactg tatggatacc aggaagcgtg
      721 tcgaaaggct ggatcataca ccagaagcag cctcctgcaa atattgctcg ttccattatg
      781 attccccctc taatactact catccgatga ttttttattt tcttttgtct tgctttcagt
      841 actgctggtg ctagttttgt tggtatttga gttgtttgaa gcatcagagg tatcttttgg
      901 caaatataga ctaaaccgaa tgtcatcatt tactaagtcg attttatctg ctttaatata
      961 catcccgttt tgcattcgga attgatccaa cctaagcatg atagtcagat catctgtatt
     1021 gacttcgatc caatctggta atttatagtt tctcttgatc atattcatca catctttgat
     1081 tggcaaactc aatgttccga tagaaagact tctagctttt agttggacat ttccgttttc
     1141 catcacatac ggatcaaaat ataaatagaa atcgatagga aaacctagta cttcgaaagt
     1201 accattgagt agtgcttcat tttccaaata aaatttatat tcaatatcac tatctttttg
     1261 atactcacta aggaaaaagc tgatgatttg attgactttt tctttatttg aattgatcgt
     1321 taagacagga tctccttgtc tttccgtaag agctggtatt tctgggagtt ctggttcccg
     1381 attagcaaaa atacggctgc ctaaaaatac ggtcgaacca agtactaccc caagaaggat
     1441 aaggaaggcg aatttccagt aatttgtttt ctttgttttg tttgaagata cgtcagatct
     1501 tgttttcatg tttattgatc atcctccttc tctaaccatt ggttttgtgt ctcgatcagc
     1561 tgatctctca ctgcatttgc catcaattga taacctaaat tatttggatg gaaatgatct
     1621 tcttcataga gcacattatt tttgatgcca tttagatttt ctgtcgtgct agttgtttga
     1681 tctgttcctt cttctgtgac acctacttcc tgattcaatc cttgataaag caaatcattg
     1741 attgggataa agtagcagtt cggcattttt tttgtcaccg tttctgttcc tttgttccaa
     1801 ttgttaacaa ttgtctgcat gtcagtgatc tcaggaaagt tcaaataaaa tggattgtag
     1861 atgcctaaaa tgtaaatagg tgcggtttta ttgagggatc gaaattcttc caatagttct
     1921 gtgacatttt cctgatagtt tttcagtggc tttttaaagg aattgaccgt taacccgaat
     1981 aaattatttt ggataacttt catcaaatca ttaccgccaa cagtcagtgt gataaaatca
     2041 gctgttttga tattgtttcg aatttcttct tttgttttta ctcgctttaa gatctggtca
     2101 cttcgttcac catttacacc atagttttct gtttcaaccg cggtcaaatt ataacggtct
     2161 tttagatcat ttgcgacaag gggaacgaat cctccttgct tggtttgatc tcctactcct
     2221 tcagtcagcg aatctccgat agctatgtag tgaagttttt cttttttatt tggatcttct
     2281 ctcacagtag ttactggttt cattagtggg ccagctttcg gtatagaagc agctaaacct
     2341 gcatagatga tgatcgcaat taataatggg aacaaaacga ggatcaaatg tcgtttcgtt
     2401 aatttcattt tttcacctct tgtattcgat tggttctaat gttatcaaaa attttatcaa
     2461 aaaggtagtc tttatgaaaa aagaaaacaa ttattttcct aattttataa agaaaaagag
     2521 aataagctgc aagcctttca ggctgagggc ttatcctctt ttcgactaat ctacataata
     2581 catgattgca aacgcgtctt taccagtatg cgtagcgata accggattcg tatgcagtac
     2641 agggatatgc atgtccggga ataattcttg caaaccctct ttgaattctt gcgccaattc
     2701 ttttgcatcc gcatgtgaaa taccgatttg tcgcacattt ggcaattctt tcagttcatt
     2761 tttcaaatta ttaaaccatt tatcgaacgt tttctttcct cgccccttag ttacagggat
     2821 taattcagaa tgggcgaaat tcatcaccac gcgtatgttc aaaacactag acagcaaacc
     2881 agttgttcgg ctgattcttc caccttttac caagttatct agtgtggata ttccgatgta
     2941 taattttgta ttgtcttgta cttttttgac ttcttcaagg attttctcta ctgttttacc
     3001 ttcttttgct aattcagctg ctcgaattac ctgaaaagat aatccttggt cagtggtatc
     3061 actgtcgata accgttactt tcgtttttgt tagattactg gcttgtcttg ctgcttctac
     3121 tgttccactc aagccttttg tcatatggat cgaaatcact tcactgccgt cttctcctag
     3181 ctcatcatac atcgagatga attctccaat tggtggttgg cttgttttgg gcagattttt
     3241 tgctttcgcc atcatttcca taaatctttc gccgtctagt tggtcatcat cgggatatac
     3301 tgttccgtcg atcatgatgg atagcggcat gacacgaatg cctaaagcat cgcgtacatt
     3361 tttttccatc gtacatgaag aatctgttac gatttttaca tttgtcatta ttttctctct
     3421 ctctttcttt aacaagatgc cttattcatg ttaaactaca tgtaagacaa tttctatttt
     3481 tagtataaca gacaaataat gatttttcat taattacacc tagtaaagga gccgttttca
     3541 tggaaaaaac gaaatttaat cgaacgtatg aaatattaaa tgaagtgttt aatgcagtca
     3601 cccatggaat tgggactgga ttgagtatcg cgggactagt ggttctttta gtaaaaggtg
     3661 cccacttgca ttcctccctc catattgtct cttatgctat ctatggatcg atgatgattc
     3721 ttttattttt aacgtctact ttgtttcatt cattgatttt tacaaaagca aaaaaggtgt
     3781 tccaagtatt cgatcatagt tcgatttttc ttttgattgc aggaagttat actccttttt
     3841 gtttgctgag tatccgagga tggctaggtt ggactttatt tgtattaatc tggttgtttg
     3901 cgattcttgg cattatatat aaagcgttga cgttgcataa acgggagaga gtatcaaaag
     3961 tatctacgat catctatatt tttatggggt ggctctgtgt cgtggctgct gttcctcttt
     4021 atcactctct tggaatgact ggtgttattt tgatggtagc aggcgggctt tcttacacga
     4081 taggcgcatt cttttatagc ttgaattcta tccgctatat gcatgttgtt tggcatttgt
     4141 ttgttatgtt aggtgcaggt tttatgtact tctctgtttt attctttact taataaaaaa
     4201 aataaagcta tggatgggac agctctaaat aacagctagt ctatccatag cttttttata
     4261 tcgtcaattc gcctgtcccg ctttttacag gattctcatt acgctgatac gtttcaaatt
     4321 gatgcggata acgatttttt tcatccattt ctccttgact agtattgatc agtgaccaat
     4381 cagaccagtc aacaggtgga aaataagcat ctccatcgaa tacatgatgg atgactgtac
     4441 ggtataatat cgtacaaaat ggcagaaatt cttgataaac agctgaccct ccagcgatga
     4501 aaaagatacc gtctgtttct ttttccagag caagcacgtc ttccacacta tgcatgacat
     4561 gcacgccttc tgctttatag gaagaatctc tcgtcaaaac aatcgtctga cgattcggca
     4621 acggacggct acccatccct tcgaatgttt ttcgtcccat cactagtgta tttgcttctg
     4681 tcatttgttt gaagaatttc agatcatttg gcaaacgcca aggaagacgg tcttcttttc
     4741 caatcaatcc gttttcatcc tgtgcccaaa ttgcagctaa cattttctca tcctttctga
     4801 ttctaaacag cgataggagc tttgatcgcc ggatgtgggt catatccttc taattggata
     4861 tcttccatct caaaatcaaa tactgactgc ttttcctgat tcaaaactaa agtcggaaat
     4921 gcgcgtattt ctcggctcaa ttgttctttc atttgttcga tatgattgat ataaagatga
     4981 gcatctccta atgtgtgaac aaattcgcca accgctaaac ctgtttcatg tgcaattagg
     5041 tgagtcagca acgcataact tgcaatgttg aacggaacgc ctagaaacac atccccactg
     5101 cgttgataca attggcagct tagccgtccc tcttgaacat aaaattgaaa catcgtatga
     5161 caaggaggca aagccatgct aggaacatct tccggattcc atgcagaaac gatcaatctt
     5221 ctagaatcag gagtcttttt gattgcttcg atcacttgtt tgatttgatc gataaagcca
     5281 ccgtcttttg tttcccagtg acgccattga tagccgtaga tgtgtccaag atcgccgtat
     5341 gtttcagcaa aggaatcatc ggttaggatc ttctcgcaaa aaagcttgct ctgcttttta
     5401 tattcttcat tgaattcagg atctgtcaaa catctatggc caaaatcagt catatctggt
     5461 ccattatagt ctggactttt gatgtagcgt tcaaatgccc attcatccca aatatggttt
     5521 ttattttgta atagatagcg gatattcgta tctcctttta aaaaccaaag caattcgctt
     5581 ttgatcaatc caaaaggtac tcgtttcgtt gttaacaaag gaaacccttt ttgcagatca
     5641 aaccgcattt ggtatccgaa taagcttaat gtgcctgtgc ctgttctgtc ttctttacga
     5701 tgcccttctt ccaacaattt tcttcctaag tcaagatacg cttgttccat cctttttcct
     5761 cctaatcttc tacccattca ctcaagtatt cccaccgtgc cattttttct tcaagttgtg
     5821 cttctgtttc agatacgtcg ttttgcaatt cttgtaatcg agtgaaatta tctccttgat
     5881 gattcatctc ttcttggagc aatgagattt tttcttcaag ttctgcaatc tcatcttcaa
     5941 tcgtttccca ttctttttgt tccatgtaag ataatttctt cttttcagca ggttccttgc
     6001 tctgtatttt tggtttcgct ttttcagcag gctgctgggt ctctttttgc ttcagtaaat
     6061 aactggacat gtttccgtaa taaagttctt gttttccgtt tccttggaat actaataact
     6121 tatctgctac cttgtccaag aaataacggt catgggaaac agtgatcaca gcacctggaa
     6181 aacttctaaa ataatcttcc aaaatcgtca atgttgctat gtccaaatca ttggtcggtt
     6241 catccaataa taaaacatta ggttgctgaa tcagcagttt caacaaaaat aaccggcgtt
     6301 tttctccgcc tgaaagtttg cctatcagtg tcccatgcat aaatcttgga aataaaaatc
     6361 gttcaagtaa ttctgctact ccgatttgac tgccatctgc tgtttttgct tcttcagcag
     6421 cttcttgtaa gtaagcaatc atccgtttat ctggtgccat ttcttcattt tcttgtgtat
     6481 aatacgcaag atgaaccgtt tcaccgatag acattgtacc gctatccagg ggaattctgc
     6541 cagctagaat attaagcaat gtcgattttc ccgcaccatt tttaccagta atacctagtc
     6601 tttcccgaga ttgaatcaat aaatctaaat gttctagcag tgtttggtca tttatacggt
     6661 aactgccatc tttgatttcc agtacttttt tccctaaacg ttgtgtcgct aggttcagct
     6721 ctaaatcatc ttcttggttt acttggtaca agttctcctt tagatcttcg aaacggttga
     6781 tccgtgcttg ctgcttggtg ccacgtgcct gtacgccagc cctcatccaa gctaattcct
     6841 gtttataaag ccgcttgcgc ttttcttctt gttctacttc tacacgatca cgttctgctt
     6901 tttctaagac gtaagcttca taatttcctt tatactcgta taattttcca aatgaaagtt
     6961 cgaagattcg gtttgctact ctatctagaa aataacgatc gtgggtaacc atcaatagtg
     7021 cgccacggta ttgtttcaaa tagttttcca accactcgat cgcttcataa tctaaatggt
     7081 ttgttggttc gtctagtaat agtaaatcgg gttcatcaat caacacttgc gccagactaa
     7141 ctcgtttttt ctgaccacct gataaagtac tgattttttt atctaattca ctgattccta
     7201 atttttgcaa aataatcttt gcgttggtat ctgtagtcca agcatcttct ttattcattt
     7261 gttcttctgc tttagcataa cgtttttgca catcagggtc ttctccgttt tcccctagtt
     7321 cgatcaatgc ttcctcatat gctcgaacag tttggatcaa ggggctgttt ccttgaaaga
     7381 ctgcttggag aacagttaat tcatcggaaa attcagctgc ttgagaaaga taaccaatac
     7441 ggtaatcagt tggataaaac actgtttgcc gatcaccgtc gctgctatcg ataccggcaa
     7501 taatatttaa gagacttgtt ttacccgtcc cgttcgtccc aatcaacccg atacggtctt
     7561 tttcgtggac caaaaaagaa atctggtcaa acaaatcttt ttcaccataa gtttttttga
     7621 ggtcagttac ttttaattct ttcaacatgt tccctcctta tcttggatat tgtatcataa
     7681 aaatagtgaa cttcaggaaa atttttttgg tatttgttct ttttgtgttc tcaaaaaaag
     7741 aacaggaccg attcgatcct gttctgatat gttttgtttt ttataaaact tgaaaatttt
     7801 taccttttta ttttattgga ctcgtttttt ttgactttgc tcgaaacgca aatcggaaag
     7861 aaaagttatc agatggtttg ttcatcgtat aagattctaa caaattgata ttcctgcgat
     7921 agctgttcgc agaaaacgtc gtgatttttt cagcaacttc gtattcatca atgatttttc
     7981 tttcaagata ataacctctt agcatttttt tcgtaaacag actatcttgc tggatcatcc
     8041 acgaagaaat caaattcccc gagttcattt ttattccttc aagtttgcgt tgctggataa
     8101 aaaattgaac catcgtttca tcataaatat cagtcaagtt atctaaactt tcaataatca
     8161 actgagtatt tttttgatat gtctctctga tttcttggat atcttcatca ctaataaatg
     8221 tctgacgatc agcatatctt ctttgccaaa acattttcgg atggagaacg attcgcatta
     8281 acctacggac aacaatgaac caaaagccga taaaagacaa aatctggctt gtgatagaat
     8341 gttcgaatcg agaaagaaaa cgggagtaaa aacgataacc attagaagaa agcttgcccg
     8401 aacgataact ttcatccaat ccatcccgtt cgatcgacaa aatcagtgct tgtatttctt
     8461 gtacttcttg cttttcatca tcggttaaat cttccaagta cagatcacgt atccgttctt
     8521 gataattttc tatcactgct tcagtggcta aaaattcttt tgtattacga tcaatttcct
     8581 ttatttcttt tcgcaaaact tcaacgacct cttctaaaat catcaaagca ttcaaatccg
     8641 tgcttttcgc ttcctctgtt tccgtcagca taggcaagac aatgatccct atgaccaaag
     8701 tcactaagat gacacccgcc gtcagaaaca aaagcaaaga ccgctggtaa aacatcatat
     8761 tattgattga aaatggcaaa ataaaaatcg tagccaaact gactgttccc tttacaccgc
     8821 caaatgttaa gatcaagatc tcgttcaact gttttttgaa ctttttactg ccatctttga
     8881 atacataaaa aagactgata gaaatgaatc tgatcacaaa aagcatgaca gagattaata
     8941 cgataatcgc taataaaaga ccgtttggat ataaaccgtc tccccatact ggcgagaaca
     9001 cttgcgtcaa ttcgattcct aaaaatataa atactaatgc attcagtgta aaagcaatag
     9061 ttgtccatgt actgtgggac aagctcgaca attctgcatc aaacacagag attttcctaa
     9121 aaccagaagc ttggagaata cctgctgcca ctgcagcaat gattccagat acatcaaatg
     9181 cctctgccaa tacataggct agaaaaggaa gcaaaagttc gatcagcaga taccctgtga
     9241 catcttgtgc tgatgctttt tcgatcaaat gaattatttt ttgtttgaac cagacaagca
     9301 aaaagccgat caaagctccc ccaatactag aaactactag actcatcccc gcgtttactg
     9361 ctgaaaaaga tccggtgatc agcgcaccaa gagcaaactg gaaggcagta acaccagatg
     9421 catcattcaa aagaccttct ccttccaaaa tatgcatcgc ctttttaggg atattcactc
     9481 gtccagaaag agaactgact gctaccgcat ctgtcggacc taacgctgct ccaaaagcaa
     9541 agcaggcagc aagtgggaca cttggcaaca ggaatgataa cgtcgcacct accccgacaa
     9601 gggtcaaaat gactcctcca aatgccaaga acaaaattgt cccgaaattt ttgagaatcg
     9661 aagaaatatc tgctttttct ccttctctaa ataacaatgg tgcaataatc ataactaaga
     9721 acaattcagg ttcaaaatcg attgattgtc cccactctgt cagacctaat accactccaa
     9781 ggaaaatttg gatcagtgga gctggaatca ttggcaaaat gcggctagct atgttcgata
     9841 aggtgatcgt tactgcaaat acaataacta actcagcaaa ttccatcgtc catccccttt
     9901 attttttatt ttgtgtaagc atttttaaaa attgttcttt tttctttaaa attttggttt
     9961 cgactacttc aacctgtgtt tggttcccct ctaattcaca ctgtacacgt tttgactcca
    10021 aacgaagcag atcatgtttc agacgttcca actgacgata catcaatata tttcttttac
    10081 gcaacttaca aagtatttga cggcggtcat ccatcgaaag ggcgccatag cgatcatgca
    10141 acagtttttt ttgttgttca aaactctctc tcattttcca gatctcctat acacaaactt
    10201 ccatcattat agcaaagttc tttttggatg aaaaatattt cattgtttca tcgtttattg
    10261 ctagttagaa atttccataa atttttcaac gcctcattct attccaaata gtatcagata
    10321 tcaagtaaaa cttaagaaag ataaaagttt ctgacaaaag gatgtttact ttcccattcc
    10381 tgcctttctt gttcttttat cacaacaaaa aaatggggct gcgacaaaaa tcttgtcaca
    10441 gtcccttatt ctgaataaac ggtgtggaag agacagccta tcagtagtaa taagacacat
    10501 ctctttcaca gcctcatttt tgcgtaaaaa tcgatttatt cgaaaaattc cccgtataca
    10561 tcttctacac gttgatctgg attgatcacg atatacaagt tacctgtttt ttcgctgatt
    10621 tttgttgatt gatattgatt gtctaatcct tctgttcctt tatctagata tgggaagtac
    10681 acaagatcag gtgtgccatt tcggtaaagg atttccattc gctcgatatc ttcgtatttc
    10741 aatgcgcgat taaacatgcc catttccagt ccgcctaggt tgatatcatg tgtcgcaact
    10801 gcgtcccctt catggaagat ttcgatttta aatccagcac acggatggat ttcaacaaat
    10861 tcactgccat tgattcgtcc aaaacttgtt gtgatttgtt tgatccaaag atcgccgata
    10921 tgacggcggt tgatcgtcca ggtttctcca tttctaaaat aaaattttag cgctgtcatt
    10981 tctttactca ttaaaaagac tcctttatga tgtaatttat tcttgtacta cctcagttta
    11041 gcacaaattt atgtaagtgg tcacatctta tgttttttct tctaatgacg tatctgccca
    11101 ctttaagtag ccaacaaagc tgagcagata cagtctagtg ttttttaaat caatttttct
    11161 ttcgataaaa aatccaaaac ctgtttttta tcatttttta ttctttgttc tacgacggct
    11221 ttttcagcta attgcaaagc ttcactaacc cacttaccag gtttttttcc agtttcttct
    11281 agcaagattt taccatctac tgctaattcg cttaatgact tgataggtaa acgatcatag
    11341 gattctccta cggcatccat ctgaggtgtt tcgtcaaaat agatcatcaa ttcttctact
    11401 tgcagtgcat gttttttgcc taagaagtac aattcgaatg gttccaaagc tccagtttct
    11461 cgttgtcgta gtccttggac aagtgcttga acgtctttaa tcatttggtt agaacacttc
    11521 cacgatttca aaaaggaacg aatctcattt tcttttagat ccagttggct gatcaataat
    11581 gcccatgctt ggctctcttc tttcaatggg acggatggta attcagcaaa acgtaaaagt
    11641 gcttcgcctt cttctttcaa accgggacag caaatgtagc actctgtttc aataaatgca
    11701 tggattcccg cggcccgttc atttcccaag agcatcttga tgaattccac atttatacgt
    11761 tctacagata ttttacctaa tagctcgtgg ttttctttga tggcgtagaa ggtttcttcc
    11821 tctaatttga accctagttg actgacaaaa cgcaacccgc gcatcattcg taaggcatct
    11881 tcatggaacc gctcatgagg attgcctaca gctcgcaaga tctgttcttc tagatcttgc
    11941 agaccatcga acaaatcgat gatttctcca tcttctttca aagcaaacgc attgatcgta
    12001 aaatctcttc ttttcaaatc ttctgccaac gaacgaacga attcgacatg atctggtcta
    12061 cggtagtcct gataagttga ttccgtccga aatgtggtga tctcatattg ttcatcattt
    12121 tctaatacca acacagttcc atgttcgatc ccaatatcta tcgtttttgg aaaaagctgt
    12181 ttgatttctt ctggaaatgc gcttgttgcg atatccacat catggattgg ctggttcagc
    12241 aagatatcac gaacacttcc acctacaaaa taagcttcat atcctgcatt ctctaatttt
    12301 ttcagaactg gaattgcctt ttggtattcc agaggtaatt gttttaattt catttttcca
    12361 acctttcctg cgtgtttctt gtcctgagat aaagactcag ttgcatggtg actgtttgac
    12421 aagtccgttt cagagtcttc aaggccagga ccacttcatc ttgtttactc gtccttacgc
    12481 tcaaagcgat tatggtctct ttgattgaat ttttccatgt ttttttcaaa aacttcagtt
    12541 aagtcgatat tcaatgaatt agccatgatc atcgtaacga ataaaacatc accaagttct
    12601 tctgctaccg agttaactgg ttcagaagcc tttttgcttt tttctccata agtgtggttg
    12661 acttctcttg ccaattcacc tacttcttca gttaatcgag ccatctgagc taatggagag
    12721 aaatacccca ccttgaattg ctggatatat tcatccactt cctgctgcat actgtataaa
    12781 gaacgctttt tatccatctt tttccctcca aaactatctt accaaaactt taacgcaaat
    12841 cctagataag agcaattgag aaaatcgaat ttccatgcta catttaggaa ggaatttcta
    12901 aatggaaatt ctgtgaaaat acataattga aagctggaat tcatatggtc aaacgaaaaa
    12961 cagtaaaaga agttcttttt atcattatag gaacaagtat ttatgcattt ggtcttgttt
    13021 acttgaacat cgctaatcat ttagcagaag ggggagtctc agggatcacc ttgatcttaa
    13081 gagctttatt tggtattgat ccagcttata cgactttatt aatcaacatc ccattgattt
    13141 tactaggagg aaagatcctc ggtaaacgtt ccttggccta tactgttcta ggtacacttt
    13201 ctttgtcatt ctttttatgg atctggcaaa gggttccact agaaatcaat ttacagcatg
    13261 atctactgat cgtttctcta cttgcgggtt tagttgcagg tgtaggaagc gggatcgtct
    13321 accgcatggg tggaacaaca ggcggcagtg atattatcgc tcggatttta gaacaaaatt
    13381 acggcatcac tatggggcgt tccctattgg catttgatat catcgtctta cttgcttcat
    13441 taacctatat cgacttgaaa cgtatgatgt atacgttgat tgtttcgtat gttttcagtc
    13501 gtgtgattga ttcgatctta gatggcggat attctgcaaa aggtctactt gtcgtctcca
    13561 acaaaagcga agagattgca ccactactga tgaccggtct gcaaagaggt gtgactttcc
    13621 tttctggaga aggcgcattc tctggtaatt caaaaaaaat catctatatg gtcgttagct
    13681 ctagtgaatt aactgaagct aagaggatcg tccacgagat tgacgaaaaa gctttccttt
    13741 caatcatcaa tgtgcatgaa gtggaagggc aaggctttac ttatttgaag ccacagacaa
    13801 gattactgaa aaaactataa gaaaaaagac aaagataggc tatgaggaaa gattccacat
    13861 ccagctgacc tttgtctttt ttagtactat actattttta actaattgtt atgttcactt
    13921 acccggttac ttttcttttg tgtctgcaaa aggatctttc ggttaacttc atcagataat
    13981 ttcaaaaaag catccctatc tccatcttct agtgcttgat cgatttgagc ataaagcatt
    14041 tttagttttg cctcttcttc tttttcttga aaatatcgtt caattaattc agtatcttct
    14101 tcactgactg aagaatccca agaagaaaat ggattgtctt ctaagataga tagatattct
    14161 cttgtctgcc aagcattttc gaatatacat tccacatata aaggcttttt ccaattcaaa
    14221 cggatttcat gaaaaatctg gtctgtgtcc gtaaattctt ttccctgcaa aaacaatttc
    14281 atgggttccc cttcaacaga tagatcactg atctgcagtc ctcgatccgc aaaattcgcc
    14341 ccttcaacaa agtggacatt gcttaaaatc gcttcatgat tggcaagata attcaagatc
    14401 caaaaaactt ctcttcggct aaaagataca tgattgacca tccacgttaa aaaattcttc
    14461 ttttcccgta catcaattag catttttctc acctctctgt taggtcatct aataatgaca
    14521 gtgcctccat atctcctggt tcaagtgtta aataataagt caataattct tgcgctcttt
    14581 tcagctgccc ttcttctctt aagaaaaagg catactcttt caagaaatct ggttcatgtg
    14641 atagttcatg ataagcctgt tcatagtgta cagctgcaac ggtaaagtct tctaattcat
    14701 tataagcatg ggctaggttc cattctgcat aaggattagc agtttcttcc atctgattga
    14761 tcacttgtat cacatcttca tatctttcat catccaagta aagattactt aaagtaagta
    14821 atgtttcatc ttgtttgtct cctacttcta aagctttcag taaaagttct tctgctcgct
    14881 gtctatcatg cagacggaaa gcattttccg aggccaggtg ataaagttcg acttggtaag
    14941 gattttcttt gattccttct tctaacactg tttgtgcttc ttcaatcatt tcttcttctt
    15001 gaagagcttg tcctagatat agatataacg attgataatg cggattaact tctcgaagtt
    15061 cttccagata gttgattgcc tgctgatttt ctttcaattg aagattcgta aaagctagct
    15121 ggaacagtcg atcatctgtc ctttcttctt tcagtgcttc ttctaaaggt acgatagctg
    15181 cttcgaattc tccttgcata cttaaggatt gtcctaaacg ttcctgcatc gagattcctg
    15241 aaatttcatt tatcccgctt gccaacaatg tttcataaac cactgctgcc tctttgaaac
    15301 gatcaactga aaaatacaat tcagctagcg caaactggat caatggttca tcaggaagca
    15361 acccagctgc ttctttcaat tttgcttcac tcacttcagg gattccgata acttggtaca
    15421 aatcagctat cgccaataag ctttgcggat agtaatcact ttgcttatcc actttttcca
    15481 ggtattcaaa agctaagtcg atctcattat cttcgattgc aatttctgct aacgggatgt
    15541 tcagtccgtc attttctggg tattggttga tcagttgttc aaaaatctgt tttgcttcga
    15601 tcaaaaagcc aatagccagc aattcttcgc ctagttcagc taatatatct gctgggtctt
    15661 cttttaatgc tttttctaaa aagatttgtg cttgtgacaa gtcttcatct gataaagctt
    15721 gcaacatttt ttcgctattt gtattcattt caattccctc ttatctcact gacgatttgt
    15781 tgaaataccg tcattgttct caataattct ttttcgtttg gatgttggta tccgtctatt
    15841 atacctattt tttctaaaac aagtattttt ttgctttttt ctgccaaaaa aagaactttt
    15901 tccaaatact ccatctttga aatctgttcc agccagtgga ttggatagcc aagcaagtcc
    15961 agccacacaa aaaagttttt tagttggaaa tcaaaaggat atgcttggag attccacacg
    16021 aaatgaaaca tcattcctaa aaattttttc atactagcag aaagtagatg gccattttcg
    16081 gtcagataaa aagcttgttc aaacagttta ccataatttg acaggttttc tgcctctaat
    16141 tgataaaaat agatcatttc ttcgatcatc ccggcaaacg gccgagtcgt taattgcttt
    16201 ttatttggat agtttttata cagattttgc aaaaaacgat gatcacaaat cattccgcaa
    16261 cagataaaag tcaaaaagtc aatctgcttt ccgtccacct gccgctcgct gattgtttga
    16321 tcataaacga tgcgttctgg cagattgatg gtttgcagca ctacacgatt tggtagctgc
    16381 aaaatcgtcc gctgcggtgt taatgctcgg cttagagaac ggatcgatac agggatcatc
    16441 catagtgatg aatcaagggt tgtatgtttt tgaaaaaaac ctgccagatc catgatccct
    16501 tcatttccaa atccgatgat aaacatggat tgattttcgg gatagcgttt gacgaaatca
    16561 agcaggttct ttaattctgt taaatgattg cattgagtgt tggcacaaat ataccagtca
    16621 ttgtttgctt gatttgcaaa taacgggttg atcttatctg caaacaaatc ataatagcgc
    16681 tgattcgtta aaaataagat tggctgatca atggaaagtt ctcttagctg actgactagc
    16741 gtttctccgt aaaacacttt cgtcaacgca tcttctactt gataatcaag ttccatacgc
    16801 atccctcctc ttcttatttt tattttacca ttagtaagca aattctagaa aagataagag
    16861 caaagctcac ctcataactt gcaaataaat aaaaattaaa taaaatacat gtgttttcta
    16921 tgtatgacat acggattcgg aatctctgtc tcttatttat tccttttctg ctaggaaata
    16981 aaaagaaaag cacttgtagc cctagagcca caagcgcttt ttcaactgtc ttatttaaca
    17041 gcgtctttta atgctttacc tggtttgaat gcaggtactt tgctagctgg aatttcgatt
    17101 tcttcaccag tttgtgggtt acgacctttg cgagctgcgc gttcgcgaac ttcaaagtta
    17161 ccaaaaccga ttaattgaac tttttcgccg ttagctaaag catcttgaat agttgaaaat
    17221 acagcatcta ctgctgcagt tgcatctttt ttagttaaat cagtagctga agcaactttt
    17281 tcgattaatt ctgctttgtt tgccatggat agattcacct cctccacaag agatatgata
    17341 gaaatatcat ttagttaaaa ctttctgagt ggtccagaag tttaaaaaga aatattgatt
    17401 aacttcaata ttctgttatc acgatatcat atgaaccgct taatagcaag gtttttacca
    17461 attttcacat gcttttttta gtcatagaaa cgtaacaaac gaaactgtct ttttcttttg
    17521 aaaaaaaaac gtcgatatgg taaaatgcct attttctcct acgagggata attcggatgg
    17581 acgtcccttc aaaagtaaat gcttttctga tttgattttc taagaaacgt tcataagaaa
    17641 aatgcatcaa ttcctcttca ttgacaaaaa cgacaaatgt aggtggtttg acagcgactt
    17701 gtgtagcata aaaaatcttc aggcgttttc ctttatctgt tggtgttgga ttgattgcta
    17761 cagcatccat aatgatatca ttcaacacag ccgaagagat acgaagattc tggttcatac
    17821 ttacttgttc gatcatttca ggcagacgtt ccaaccgttg tttagtcact gcagaaacaa
    17881 aaacgattgg agcgtaatca agataccgga attcatcacg aatctcttgt tcgaaatcac
    17941 gcattgtatt ggtttctttc tttacaagat cccatttatt cacgacgata ataattccac
    18001 gaccagcttc atgggcgtaa cccgctactc gcttgtcttg ttcccgaatc ccttcttcag
    18061 cattcagtac catcaaaacg atatcagaac ggtcaatggc acgcattgca cgcatcacgc
    18121 tgtatttttc agtattttcg tatacttttc cgcgtttacg cataccagct gtatctatca
    18181 ttaagaattt ctgaccactt tcagaaacga agtgggtgtc gattgcgtct cgggtcgttc
    18241 cttcaatatc cgaaacaatt acacgttctt ctcctaaaat cgcattgatc aaagaggatt
    18301 taccaacatt tgggcgacca atcaaactaa acttaatcgt gtcgtcgtcc tcttcttcag
    18361 tctcttcaga gaaatacttc actgcttcat ctaaaatatc accaatccct agtccatggc
    18421 ttcctgaaac aggataagga tcgcctagcc ctaaggaata aaattcatag atatcgtttc
    18481 tcatttctgg attatctact ttattgactg ctaaaatcac tggtttatta cttctgtata
    18541 aaattttcgc aaccatctca tctgcgtctg tcacgccttc gcgtccgctg actacacaaa
    18601 tgattacatc tgcttcttca atcgcgattt ctgcttgatg cttgatctga tccataaaag
    18661 gttcatcgcc aagatcaatt ccgcctgtgt cgatcacgct gaattctcga cctaaccatt
    18721 caccttttgc ataaatacga tcacgagtta ctccaggagt atcttcgaca atcgagatac
    18781 gctcaccagc gatgcggtta aaaatcgtag acttaccgac gttcgggcgg ccaacgattg
    18841 caattgttgg atttgccata ttatttcctc ctcacaagtt tccaatctca cttagtttaa
    18901 cgaatgcatt gccaactttc aagcatagat tgtcaattaa acgaagtatt tttattcctt
    18961 attgaaatac taatcgcttt tcaaaagaaa aactgagaca aaagcagctg ctttcatctc
    19021 agtcacagat acaaaatcaa ttatttttca gaatcatcgg ttgctttttc ttctacttct
    19081 tctttcaacg catcacctaa aatgtctccc atagtaaatc cagtattttc ttctggtaat
    19141 tcataagact caacattttt tggttcttct tctgaagctg gtttttcttc taaagctttg
    19201 atactcaatg caatacgatg ttcttctgga tggatctcta atactttcac tttcacttcg
    19261 tcaccttcat gaagcacttc atgtggtgtc gcaatatgtt tatgtgaaat ttgagagata
    19321 tggactagcc cttctactcc agggaacact tcaacaaagg caccgaagct tgtcaaacgt
    19381 ttaactgttc cgtctaatac tgtgccaact ggagcttttg tatcgatttc tgaccaaggt
    19441 ccaggctgtg tgtctttgat tgacaaggaa acacgttctt gttcaggatt gatagacaac
    19501 actttgacat ttacatcatc attgacagcc aatacatcac ttggttttgc tacatgacta
    19561 tgtgaaatct ctgagacatg gactagaccg tcaattccgc ctaggtcgac aaatgcgcca
    19621 aaatcagtaa gacgtgcaac acgtccactg agaacatctc cctcatggat agttgaaaga
    19681 atttcttttt tcttttcttc tctttcgttt tccactaccg ctttatgcga taagatcaaa
    19741 cgattttctg atggttcgat ttcgatgatt ttaaagctca atgttttgcc tttatagtct
    19801 gaaaagtctg caacaaaatg atcttccacc attgatgcag ggacaaaacc acgtacaccg
    19861 acgtcaacga ctaaaccgcc tttgacaaca tttgtaacag gtgcttcgat gaccttgccc
    19921 tcttggaaat ctctttcgat atcttcccaa acttttttag catctaaacg gcgttttgaa
    19981 agtagatagc tgccgttttc tttatctttg ccgatcgtac tgataacgac taaatctaat
    20041 acatcaccga cttttactac ttcgctgata tcttctaccg gcaaagttga taattctttt
    20101 gccggaacga ctccttcaac tccagcccct tcgattccga caatcacttg tttgtcttca
    20161 atgactaaaa cttcgccctt aacaacatcg ccaactttta cttcttgtac gcttttcatt
    20221 gcgtcttcca ttgactggtt gttctcttga ttttgatcaa attctgtcat aacacttgtc
    20281 ctcctaacca acattctgcc gtaagtacgg tactgttttt agtttaattg attattcaaa
    20341 aaaaataaag cgatttcgtt cgttttcctt gaaaaaacaa ttgtttttca ttttttttct
    20401 taatttaaaa aagaagataa ccttttttca aaacaacagc ttttattgct gcaacgactt
    20461 ctggaatcga tttgccagtc gtatcgatac gaacagcatc ctctgcttgc ttcagcggag
    20521 agacgtccct tgttgaatca taatggtctc tttcctgtat ctctttcgtc agtgtttcaa
    20581 gatcagtcgg aattcctttt tcttggttct ctttgaaacg tctttctgca cgctcactta
    20641 cgcttgctac taaaaagatt ttcacttctg cattcggtaa tacagctgtc ccgatgtctc
    20701 ttccgtccat gacaacgcca ccgcgttccc ctatttttcg ttggacagca actaattctt
    20761 ctctgacttt tccatgtgca gagacttcag aaacagcttt tgtgacatct ggttgacgga
    20821 tttctaaagt cacatctttt gatgctatga atacatgctg tccatcttct tgttgcgaaa
    20881 aagagatagg atactttttg atcagcgaaa ctagcccttc ttcatctgaa aaagatacat
    20941 tgttcgtaat agctaaataa gttactgcgc gatacatggc accggtatct gtataaatat
    21001 aattcaattc ttttgctaat atcttggcaa cggtactttt cccagatgaa gcaggcccgt
    21061 cgattgcaat actgatttgt gtcatgatat aactcctttt tctcagaaaa aaagccgcaa
    21121 agcgcggcta ttattattta attcttaatt cttgaccagg gtaaagcata aagttgtttg
    21181 gatctaagcc attcaattca aacaattgat cagtggtgat ccctgcacgc tcagccactt
    21241 gttttggacc ttcgcctgct tgaacagtcg ttgttgaacc ggctgcagct gatgaagaag
    21301 gtgttgtttc tgctgcattg ctgcttgaat cccctgcttg ataagttgcc tcacttgagg
    21361 aagtcggtgt tgtctctgct gcaatactgc tttctgacgt tacacctgta tcttgagaca
    21421 cagtagattc ttccgcgatt gtactagatt ctactgttga agtctcagtt gactcctctt
    21481 ttgttgtact tgtagaactt tctactactg atgtcgaagt ttcagaagaa gttgctgctg
    21541 ttgcattgtt gcttgatcca tttctgatcc agaaataagc acctgctgga atagcgatac
    21601 ataagaccag taaaatcaaa aatagcgaaa gaaataatgt atttcctttc ttttgttgtc
    21661 tctgagaact tcttgatgaa acttcatcat ctgtatcgta gattggttgt tcccatggtt
    21721 cttgtgtttc gttattttct gtagattgat gtctatcttt tctgctcact gttttacctc
    21781 ctaaactcta tcgtgtgtat tataccatag gaaaaagtgt cttgtcctaa aaaatcaatg
    21841 ttccatgttt tttttaaata aatttagtaa aatcgttccc caatgaagtt gttcagtctt
    21901 agaaaaaagc ttcctctttg gcagaagttc tgcttttgct ccatcaatat cacaacaatt
    21961 ctgcggcttt tcggagagtt tttcgccaaa ataagctaag atgaattttc tgcggcagtt
    22021 ttcttcatta atatagcgaa gcatctgctg caggcggaat tctttttctt tttcctgacg
    22081 tttgagccgt tccacccaat tttctggttt ttcagactgc tggatcagct ccatccattt
    22141 tttttgcagc tcatcaaagg gagcttgctc tgtggcatat tccaaataaa gctcaaaact
    22201 ttgtcgctgt tctctagaaa gttgattaaa gaaataatgg atacgttcat ccccactttg
    22261 ataaaaaaga attgctgcac tttcttcctg atctctgcct gctcgaccga tttcttgtac
    22321 atagttttct agactgtctg gcaaatcata atggaccaca taacggatat cagatttatc
    22381 gatccccata ccaaatgcat tcgtagcaat gagaaactgc agctgatttt ttacaaactg
    22441 ctgttgaagt tgtcttcttt gagctgcatc aagaccaccg tgatagtatc caactgtaaa
    22501 acgttccctg aataaatgat acaatcgttc gacttctttt tttgttgcac aataaatgat
    22561 tgccgctcct tgtgctcttt ccatgaagtg ttcaagttct tgttcttttt gctgcgtttt
    22621 ccgaacaaaa agcgagatat tttgccgatt gactgattgt ctgatctctt ttggtggtcg
    22681 atcaaatagt acatgacgta tatctcttgc tactaaatct gttgctgtcg ccgttagtgc
    22741 cagtgtgacc gggtttccta aaagctttcg gatttttcct aactgttgat attctggacg
    22801 gaaatcaact ccccattgag acacgcaatg agcctcatct actacatata acgcaatatt
    22861 ttgttttttc agatgctcta aaagagaagg attagtcaac atttcgggac tcaaaaacaa
    22921 aaatttatat tgcgatagat gacggaggac gtatcttctt tcagattgcg aaagtaaact
    22981 attgaaagca gctactcgtt tctcccctcg tttttgtaat tgcgtcactt gatcttccat
    23041 tagagaaagc agcggtgaca cgatgatgac cagaccttcc attaaatacc ccgtcaattg
    23101 atagcaaagg ctttttcccg ttcctgtagg caaaatagcg agagtatctt ctccatcaag
    23161 caacgcctga ataatttctt cttggccggg acgaaatgaa tcaaatccaa aatatttttt
    23221 taattcattt attaacattc tgtatcgctt cctttctcaa tagatagatt tgatacaaac
    23281 gaaattctcc ataatctaat tgtccatcta cattccattc gctaaagcgc cactctcgca
    23341 cggaatcctt atttggttcc aaacgttcga ttgtttctgc agagagaatc cactcgtatg
    23401 gaaaatcatc aaaaaacaag gcccactcaa taagatgatc tgttactgtt cctcttttta
    23461 aatgacggat tgccatgatc tggtcaattg tctgaccatt cataaacatc tgcttggtga
    23521 tcatcatgct ttgattgaaa ttttgctgta ataatgaatc gatcaaagcg tgccaaagcg
    23581 aatctggccg tttttcgatt tgtgctaaca aaaggtggat acatctgctt tgttgcagat
    23641 aaaccgctga ctcatcattg ttttttgcca gctgataagg aagcagccct gttcgctcat
    23701 ttccagataa ttggttagct aaaaaatctg ctgcttcttc tggtatctcc gaaaacagct
    23761 gcaccaaact ctcatacgca gtatcaatca aaatttcacg tggcaattgg ctttcactaa
    23821 gccatttttt tagttggaaa gtataaaaag gacttgtctc tgctggaaga tagtcttgaa
    23881 tacccgttgc cagattagaa attacttgaa cagcaaattt aatgagccgc caactagttt
    23941 cccctgttct gccatagcgg tcaaaacgca aaccagtatg ttctatttga tgttcggaca
    24001 acatatcagc ccctgcaggc gttagctttg cttcattttc atttatttcg atccaacctt
    24061 gctgcgcaat tttttgcaaa atttgataaa acttatcctg cttcaattct ggaaaactgt
    24121 tatggataaa caaaagttcg tgggtaaatc cataaataag tactgaagtc gttcgttttc
    24181 ccacaaggag ttggtaaaga gaactaatcc gcagcttgtg tcctgatgaa aatagcgata
    24241 aaataaaaac aatcatatta ttctccttaa gttaggatgg ttagaaatgt cactactatg
    24301 taaacttgta cctgaacgct gtattgcatg cggtctttgt caaatcgaag cgccagatat
    24361 tttcgattat gatgaagaag gaatcgtttt atttgcccaa gaaccagagg ctcatcaaca
    24421 gttcgtgatg ccggaagaag aaacatatgt aaaaaaagcg tatcaaaaat gtccagttcg
    24481 agcgatttta ttagaaaata aaatataaat ataaacgaaa ctttaaaaac gtacactaag
    24541 ttctctaaac ttgaaaataa gccgggatct gtttctcttg ttccgtgaaa tcccgactta
    24601 ttttgaaaga ttcatttgct atctatagtt aattgactag ttttttcacg gaccctatag
    24661 gacccattta tattatttca actttttcag ccagttttcc aatgtattga cgatcacagt
    24721 ttgctggtct gcgttagaaa tttctgcctc tccatctcct ttttgctcac catagctgcc
    24781 aaaacctcca tgatttccgc cattgattga tacatactct gttctttccg gtaaatactg
    24841 cttagcttct tgataagctt cctgattcaa tactttatct tttgtcgccg tgatcgataa
    24901 aacaggaaca gctgattctg tcaatgttcc ttttttgtcg ggataacttg ccatgaagaa
    24961 tacccctttt aaatgtttgt ctgaatgatt cttattgaca taacgacttg ccattacgcc
    25021 acctaatgaa tggccaccga tcacataatc ggaatttgga aaatcttctt tgaccttatc
    25081 cgctttgttt ccgcccatca ctgctagatc aagtggcatt ttgacaatag cgactgaaaa
    25141 gccgtttttt gctaaatctt gcgcccaaat actataactt tctggctcta cgagagcacc
    25201 aggataaaaa ataagtaatg gagactttcc atgattcttt ttctctcctt caaaaaataa
    25261 gtaatcttct ttgttttttc cttcttttac caacacttgc gcttctgacg atggttcgta
    25321 cgtattttgc tgtatatagt aataaccggc tccaccgagt aatcctaaaa tgatgactaa
    25381 gccaattagt attcgtctaa atattttcac tttctcccac cttttctatc tgagtgaccc
    25441 ctttttctta atcaagtatg attatacctt aaaaaaataa gagagaaatg caaactttcc
    25501 agcatcaaat aaaaaaagac ggaatatcca tttgaatatc cgcccttttt gtatgtttat
    25561 ttttatttag cacataaacg atacacagct tctgcataaa tttccattgc tttatacata
    25621 ctacttaatt cccatcgttc attcgcctgg tgcataaagt caggagtatc agggaacatc
    25681 gcaccgaatg ccacacactg gttcatcgta cgagcaaaag tagcgccgcc tgatacaaac
    25741 ggttcagtca tgtctccagt aatatcgcga tatgtcccca atagattttt cactaactca
    25801 ctgtctagtg gcacgtataa tgaatcaagg aagtcaaatt cttcataagt caacccatat
    25861 ttttctgctg ttttagttaa tttagctact aaatcatctt tcttgaatgt aacagggatt
    25921 ctcatatcta caccgatttt tgtttgttct ggtgtgattt ccaaacttgc aaagttcatc
    25981 gttagctcgc cggattgttc atcttctgtt ttgcccacga cagcttcacc agttgcattt
    26041 tcttgaacta attttcctaa gaaattgatt gggccaaaat caaatacttc tgaaagagca
    26101 attgccagac gagaaatagc attcactcct tgtgctgcat ctttcgcgtg gatactttta
    26161 ccaagaacga cgatgccttc accttgttct tcgaaatcga attcgtgttt tttcaatgct
    26221 tcttttactt cagaaagctt ttctccagag taaggagctg catcaggaac aacattcaac
    26281 gctcctccag cttttacaga aaactcgttc gttcctggtc ctgtcaagta agcttgcaat
    26341 aagccttttt ctgcataaat taaaggaaat tcagcatctg gcgcaaatcc ttgcgtaatt
    26401 ccttcttctt tttcgttata tttttctaaa caacgccata aattttcttc atctgtacca
    26461 aagataaaac gaatacgtgt cttgaattcc acgccagcat caatcaaagc tttgacggca
    26521 tacattgccg caatagaagg acctttgtca tcttgggatc cgcgaccaac taaccagccg
    26581 tcttttatag ttggatcgaa tggttttgtt tcccaattat tttcgtcccc tgctggaaca
    26641 acgtccatat gacacaagat accgaataat tcatctccag agccaatctc tgaataaccg
    26701 taatagcctt ctggatcttt aaatgttcta aatcctagct tttcactgat ttccaatact
    26761 ttatccaaag cttcaatcac ttttttaccg aagggatgtc cttgtcctga atctgcttca
    26821 tctagtacgg aaggaattcg gatcaattcg ctcaatgatt ctaatgcttg ttcatgatgt
    26881 tgttctgtca caaatttttt catgatatta tcctcctaat atgttaaaaa gggtcatgtc
    26941 aaattcctcc gacacaacct cttcttctca aataatgacg ttttaagcct gctccatcag
    27001 caagcagaat gttcttaaca aatgatgaaa gaaaaaacac tcactgaatg gagctattga
    27061 cttatccacg tgcttatcga tagccgttta tcggtttctt cattcttatg atagaccttg
    27121 tacctgtatt tgtctaggtt tagaacattg ctgctacacc taggatcaca cagatggcta
    27181 caaataatat cgcaattaat ttaccggtaa atttccacca cactgaaaca tcgaaacgag
    27241 caattgctac agcacccatt acgatagcag atgttggtgt aattaagttt actaaaccag
    27301 atgcagattg ataagcagtg attactaaat ccttaccgac gccggcaaat tcacccatag
    27361 gtcccatgat tcccattgtt gcagcagcta agccggaagt tgaaggaatc aagaatgaca
    27421 tagggatata gaaaataaat gtaataatga tgaatacact tgaagataat gaacttaatc
    27481 cttgttcgcc ccaatgtaag atggtgtctg tgattaatcc attgttcata accacttgga
    27541 taccacgagc aacagctact acgattgcta caccgatcaa atcagaagca ccagctaaaa
    27601 attcagagac aaattcagac tctttcatgc cataagtgat tgctactacg atggacatta
    27661 ctaagaataa catagtaatt tctgggaagt accagtcacc taatggaagc atgcttttac
    27721 ctagaaccgt acctagtacc ggaaggtttg ttaaccagtt tgtcaatgat tcaaaaatcg
    27781 tgaaattcgc attcaaggtt gtccaaggaa tcaaactgat gatcatgata ccaaaagtaa
    27841 taaagaacaa tacattaaca tgtttttgtt ttttcgtgat tacttcttga cgaccgattg
    27901 cttcaatatc gaaatgcttc atatcttctt tacgttggtt gtatactaat gattttgttg
    27961 gatctttttc gatctttgat gcataacgat acacatatac aatagaaata gctaacaaaa
    28021 taacgaacat caagaatcga agaccaatac cttcacccat cgaaatccca acagcttgtg
    28081 aagctacgcc tgttgcaaaa ggatttaccg ttgaagctag acagcctact tgagacccta
    28141 ctaatacgat tgctacagct actactgtat caaagcctac agccatcatg actgggatca
    28201 gtaaagcata gaatggaatc gtttcttctg ccattccgta agtagagccg cctaatgcaa
    28261 ataacagcat caatactgga ataagcattt tttcttttcc tttgaatttc ttcacaactg
    28321 aagcaatccc ggcatctagt gcccctgttt tgttgataat acctaaaaat ccaccaacaa
    28381 ccaaaatgaa gaaagagatt gggattgcag caggtgtttc tttagttagc tcccccgctg
    28441 gtacacctag catcccattg attggcgcca tgaaaatatc ccacagccct tgaggatttt
    28501 gttgtactcg ttcatatgat ccagcgacga tgttgcctgc atcgtctaca ctatagtgcc
    28561 cagctggtat aaaccaagta agtacagcga ttactgcaat aataataaat aagaccgtat
    28621 acgctgaagg catttgaaat tttttctttt ttgtttgttc catcttcttc ttcctttctt
    28681 ttccgttaga gacaacaatt ttggctctaa tgttatttta tcaaaaagag tatcagaaca
    28741 aaaataataa cacgcatttt gtaaaacttt tgttcaaaaa gttcgttttt ccctattccc
    28801 tcctcttttt tcaggttttt aaagctttct tctttttcat tgtagcgttt cctttttttt
    28861 ataaacaaac attttcgtct tttaaaagga gaagaatttc acatttgcaa agaattgatt
    28921 gatttttatg cataaatcaa aaaaatactc aaaacaaaca aataaaacgt ttacattatt
    28981 gcatgtcttg tatttttttc caaaaaaaaa gaccaagaaa tttcttgatc tttttgatat
    29041 ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta accatggaag
    29101 aagttgcgca tgcagcacta aaaagacagc actcacgatg aatcctttga tcaagttgaa
    29161 aggaacgatc ccaatcagta catagtttgc taaagacatg cccagcatct gtgttgcgtt
    29221 cagccctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga tactcataaa
    29281 aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc ctttatgctg
    29341 tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg ataagaaact
    29401 agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat gtaatactga
    29461 acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc tgatcaatac
    29521 cgagatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg gaaaagcaaa
    29581 atactgtaaa actaatgcga tcgcagcaaa catcgctact gcaaccattt tctgtacacg
    29641 ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt tttgacgttt
    29701 ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta tttgatcaag
    29761 cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc ggaatttcac
    29821 cgattcagct atttttttgc aaaataggtc gtggactata accaccggtc gggaatttca
    29881 ccctgcccct gaagacgaac cttatataat ttttgtttac atattcatta tacatatcta
    29941 ttcaaaaaac gcaacatcta acacttataa aaagtaagtg ttcattttga gtggttcagc
    30001 aaactgctga cttctttttt gttcagttca cggtaatccc caggacgtag gccttgtaaa
    30061 gtcagatctc cgtatttttc acgttttagt ttttgcacag gaagaccaac tgcttgaagc
    30121 atatttttca cttgatgatt acgtccttcg tggatcgtca attccacgat actgtggttt
    30181 gtcttaggat ccactgataa aatttggaat ttagctggag atgtttttcg tccttcgatc
    30241 tttattcctt ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt tacctttgca
    30301 acgtaaactt tatccacttc atgttttgga tgtgtcaggc gctgagcaaa atctccatca
    30361 tttgtcagta gcaagatccc cgaagtatcg taatccagtc gacctacagg atagatacgt
    30421 tcttttacca tcggcaaaag atccgtaaca acttttcttc ctttgtcatc tgcaacagca
    30481 gaaataactc ctttaggttt gtacaataaa tagtaaccgt attcttcttg ataaattggg
    30541 acttcatcca cttccacagt gtcatgtttc ccaactttta tccctaattc ccttacaact
    30601 tgtccgttta cttttacacg tcctgcaagg atgaattctt ctgcttttcg acgagaagtg
    30661 atgccagcgt gggcaattac tttctgcaat ctctccatta caattattct cctttattca
    30721 ttccatcaaa gaatagatct ggtgacacgt cttcttcaag tgaaatttcc atttcttgtg
    30781 tatcaggcag ttcttctaat gatttcaaac caaaataatc taagaagtag ccagttgttc
    30841 catataatat cggccgacct ggtccttcta ctctaccttt ttcttcaatc agtttatggg
    30901 cagctagacg ctgtaccgct cctgaaacct gaactcctcg aatctgctcg atttcagctc
    30961 ttgtgattgg ctgtttataa gcgatgattg ctaaggtttc gacggcggct tgagataatg
    31021 tgtttgatgt cccttgcgca tatcgcttaa gtaagggagc cagctctttt ttagtcgtta
    31081 atacataatg gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt tgagtgtaag
    31141 attcttttaa ttctttcact aaacttgtga cttctgcttc gctgagattt aacagtccgc
    31201 ttaattcttc attactgatc ccttcctcgc caacaataaa aagaagggct tcaagttcac
    31261 ttaatttcgt catgttcggt atctcctact gctggataaa gcataattga ttcatagttt
    31321 tctttttgtt gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc cataaaagta
    31381 gtgacaactt cacttttaga aggtgactca aaaaaagaat cgaaagtaca gccatgcgca
    31441 tttcttttta tacgctcttc gataaatacc attcgttctt cgatcgttgt gtcatcgggc
    31501 gtgatcgttt tttccacttt ttgagaaaat ttcttacgtt ctaagatttt ctgcatggca
    31561 cggatcaaat cgattttttt gacttgtcct cgcttcaacg gcttttcagc atccgctaac
    31621 ccatcgatgt tcataggttc ttttgtgtaa taaagacttc gttcctctgc tttttctgtc
    31681 agtctttcag ctgcatattt atatttacgg tattctaaaa gctgagtgac caattcttct
    31741 cgaggatctt cttcccataa ttcttcatcg tctgtgacga attcttggac aggaagcaat
    31801 gtcttgcttt tgattgccat caatgtagca gccatcacta agtattctcc tgcaacagct
    31861 agctcgagtg ttttcattgt atggatataa ttcatatatt gctccgtgat cgtagcgatc
    31921 ggtatatcat atatatcgat ttccatttgc tgaatcagat gaagcagcag atcgagagga
    31981 ccctcgaata catctaattt aatattgatc tccgtcaact cattcactct ttcttctttt
    32041 tctttgctag ccattttcca atgtaaggct ctgtgatttc agttgcacgg atggagacat
    32101 ccgggtactt ttcctgtaat tcatctaaca tttgaaagcc catattctca ccgcgataag
    32161 acggattcaa agaaatgtca tggatgatca gctgatcgtt cgttgcccag taaataccaa
    32221 taatcccttg aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca taatcgtctt
    32281 cagattcgta cgtatcaatc tccttcaaca aagaagtttt acctgtcagt ttcttatgga
    32341 aactcaatag tcccatagca attttacggt tttctttccg gtaatgtgtt aacatcttct
    32401 caccttccta tgactaagcc cttggaaaat gctgcttata aacatccgtc attcttttct
    32461 tcgtgatatg tgtataaatc tgagtcgtag agatatccgc gtgacctaac aattcctgta
    32521 ctgtccgcaa atcagctcca ttttctagca gatgtgtggc aaagctatgc cgcagcgtat
    32581 gtggtgttac ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga tttttccaga
    32641 ttccttgccg agaaagacct ttcccatgat tattgacaaa tacatgcatt tcagatgcgt
    32701 cctttacaag taatggacgt gcttcgtcta agtagcgttc cagccattgg atcgcataat
    32761 cacctaaagg aatgatccgc tctttatctc cttttccaac tgtctgtacc agtccaagag
    32821 aaagatgaag atctcccaat ttcaatccaa tcaattcact gacgcgcatc ccagtagcat
    32881 acatgacttc caagattgca cgatctctta tccctaacgt ttttgtcgta tcaggtgctt
    32941 caatcagctt ttctacttct gtcagagtca atgtacttgg taatttctgt gctttcttcg
    33001 gcgtatcgat atgttgcatc ggatcatgat cagtgatacg ctcttgccgt aaaaactgat
    33061 ggaatcttct gagacttgag atcatccgag taacagttgc tgtcgctttt ttttcttcat
    33121 gcaacttctc cagaaaacgg atgactaaaa agcgatcgac atcctgccat gaagcaattt
    33181 tctgctcctg tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt tgtcgtgtat
    33241 tggcggacaa tccccgttca atattcaagt agtggagata atcgttgatt tgctctttca
    33301 taataatccc cctcatgcct ctctatcata tcagataccg cttcattctg atagtctttt
    33361 tccataatct gagcaaagaa aaagccgtct agacactcag acggactggt ttcttttttc
    33421 tttttcatag cattcctgac agattccatg gaacgttagc cgatgatctt ttactaaaaa
    33481 atgataacgt tcttctacta cttgctccac ttcaagtaaa agatcctctt ctacttcctc
    33541 gatcgtgccg cagttcaagc atagtaaatg atgatggaaa tgtttggcgc cttcttttct
    33601 caaatcataa cgggctaatc catcattaaa acttacttta tcgacaattt tcaattctgt
    33661 caagatttca agggttcgat aaacagtagc aagtccaatt tccggactct tttttttcac
    33721 taaaaagaag atttcttctg cagataaatg ctctttctca ttttccaaaa gcacaagcag
    33781 agtagcttct ctttgcggtg tcagcttaaa ccccgcttca tgtagctgct gttttgtttt
    33841 tttcatcgcc gaaatagcat tcatgaccat ttactcaacc ccaacaattt ataataatta
    33901 caaataaaaa aagatataac actatgttaa ttaaaattat tataagctag ttaagaaaaa
    33961 catgcaaggg attctcaatt agattttttg actagttttg tcacaccgtt ttcttgtgta
    34021 atcaatcctt gtttcaaaag attacctaat gcgcgcttaa atgctccttt gctgatacca
    34081 aaagctttca tgatttcttc tggatccgat ttatctgtaa agttgatttg ctgatctgca
    34141 gaacgttcta aaatggttaa aatcatttgt gcatcatctg atatagcttc atatgctctt
    34201 ggcttcaatg aaagattcag tacaccgtct ggtcggacac caatcactcg accttttatc
    34261 tgctcgccta gacgaggttc attatatcgt tcagaaggat ggataaaccc aagatagaaa
    34321 tcttccgtta aaacgtacgt acctgttaat ttcaagcggt atgctgttcc tatcacatct
    34381 ttgtttttca tttcttctgt tccatatttg cttaaagatt tgaatatttt ttcatctgct
    34441 aaactgcccc aaatacggtc ttttgcatca actctaaggg aaatcatcaa gcgatcccct
    34501 tttttaggcc atagctctgt cattgtcggc aattcatcaa gagatacgac aaagtcttta
    34561 tctggaagac cgatatcgac aaacgcacct aaatcttttc gtgtagcagt aacgattcca
    34621 aaagcgtaat gaccgatacg gcttttagga atttgtgtag tgatccgatt ttcatgtttt
    34681 tgattttggt aaccgaatcc ttcaactgct tcaccgattt tgtgttcacc ttctgatttg
    34741 cttaagccaa aggtttgtcc gtttttttgg acaaagtagt ttttttcatt ttcatcaata
    34801 ataatacctg taaatatagt tgctaataaa tcgttcattt ttttctccct ctatagtgaa
    34861 aggttttctt aatacatttc tttgctatca taacataatg ttatctactt gaatagctga
    34921 gccttggttt ggaaaggtga aaaaaggagt atgacaacag ttgtgtcata ctcccctttt
    34981 tcgaataaac ggtgttaaac ggcaggctca cagcctcttt catttgccta aaattaattg
    35041 ctcccatttc ctccgaacca tccagtttta cagttgatgg ttacgatgta aaatggcaaa
    35101 gcactttctc caatgccttt catcaatgca gattttgttg aagtggcata tagattataa
    35161 tagccttccc cattttcgat tctggctttt tctaatctgt aacctccttc aacgatatat
    35221 ccacgttgga tacatgtagc aatcactttg ctttggacac ctggtgccca cgtccatgct
    35281 ggattagatg catcggcaat atcacttgct ctggcagatt tgattggctg attcgctgag
    35341 tttgaagagg aaggggtcga ggatgccgct tcttgctgtg ctgcttgtgc gaccgcagct
    35401 tgttgagcgg cttgagctgc agctgctgct tgagcggctt gttttgcttg ttcttcagct
    35461 gcttttttcg cttcttctgc ttttttcttt tcctcttctt cttttttctc tttatcagtg
    35521 agcatcttat ctacttgttt caattgttca ttcaactgag cagatagatc tgtatttttc
    35581 actttttcta ctgcttcttt tgccgtcttg tattggtcgc gtgttgctga atccactact
    35641 tttccgtctt taaagactat agctgttttt tctttagctg tttgcaattc agcatattga
    35701 tttttagctt ctgtttgtgc ttgttgaatc aattttccaa aatctgtgtt atccgtttcg
    35761 attgcttgga tctctttatc tgctttcaac ttgatgtcat ctgctagatg atcatcgacg
    35821 atggcaggtg ctatgaacca ctcattcact ttttggatat ctcggatctt agattggata
    35881 tcttggtaag ttgtttctaa ctctgaatat cctttttcat tcttaagact ggataaatca
    35941 ttttttagtt ttgataaatc ttgatgaatc atatctgcac gaataaattg atggtgatca
    36001 tctgtataaa aggcagcaag tttctcatga agttcttcca tttgttgctg tctttttgct
    36061 tctgctgcta cttcggcttc tactttttga tgatggtcat aataagccca gcttcctcca
    36121 ccgagtagaa gtactgctgc taaaacgaga taaggtactt tttttgattt tttcttttgc
    36181 gctttatctg gatctgtttt ttctaagttt gattcttgat gtgttttttc agtatctggc
    36241 acgattgtta ttgatttcgc ttctgcttca aacagcttag gctggttctc ttctttttct
    36301 atttttgctt caagatcagt accctctttg cttacagatt gattttcagc atcggattca
    36361 ctatttattt catcgggctc tgttgacttt ttgtctgtgt tttcgagtat ttcttttttc
    36421 tctgcttccg gcagtttatt cgttttttcc ttttctaatt gtttttcagt aaatacttca
    36481 gttttttctg tgatatccgt ttttacgtct tttattttta ctgtttcttt acctgtggcg
    36541 ttttctctat cagagtcttt ttcagtttct tgttcctttt ctggctctac tttgttttcc
    36601 tgtgcaattt tttcactgat cattttgagc agctctttcg cgtgctcttc ttttgtatct
    36661 tcacgatgtg ctttgatata agcggatagt atcgaatttt cttcctcatt atcgacgatt
    36721 tctttcagtt cagctactct tcttttttgt tttgattcaa gcgtttcttc ttgattcttt
    36781 cctgattctt ccgaagtctc atgaacagtt gctttcttct gttcattttt ctctatatta
    36841 gttacttgat cgtgagcagg tggttcttcg tcttctggtt ctgctttttc gaataattcc
    36901 attacagatt ctagaggtaa atctttcaat tccgaccatt cgattggatc gtttaattct
    36961 gtatcaggaa agactgcatc ttcagttgat ttatcctgca tagctgcatc tgtatttgtt
    37021 tcttcaattg attcttttat ttgctgatct tctgcagtta tttgatgacc gcagtctgga
    37081 cagatagtat tctgctccat ttttttcgat ccgcaatttg gacatttttt catcacttag
    37141 cgatcggctc ctttactagg tttacgctac catttaatct atcatattct atacgtcacg
    37201 ccaatagctt atttttagga tttacttaaa aataatgaat gaatttcctt cctatagaaa
    37261 aaaaagcttg agactgcgta gtctcaagct ttttttcttt taaaataaac caaacagttt
    37321 tactgatttc tcttctggtc aatagtccac gcttattcta agaaatcttt taattgtttt
    37381 gaacgagaag gatggcgcaa ttttcttaat gctttagcct cgatctgacg aatacgttcc
    37441 cgagtaacac caaatacttt ccctacttct tccaatgttc tcgtccggcc atcatctaaa
    37501 ccaaaacgta agcgaaggac attctcttcc cgatcagtca atgtatctag tacatcttcc
    37561 aattgttctt ttagtaactc ataagctgcg tgttctgctg gacttgttgc ttcctggtct
    37621 tctatgaaat cacctaagtg agagtcatct tcttcaccga ttggcgtttc aagggaaaca
    37681 ggttcttgcg cgattttcaa gatttctcgg actttttctg ttggaagatc catttccgca
    37741 ccgatttctt ctggcgtagg ttctcttcca aggtcttgca gcaattgacg ttggatacgt
    37801 atcaatttat taattgtttc aaccatatga actggaatac ggatcgtacg tgcttggtca
    37861 gcgattgctc gagtgatggc ttgacgaatc caccatgtag cataggttga aaacttaaat
    37921 cctttgcggt agtcaaattt ttcaactgcc ttcatcagtc ccatgtttcc ttcttggatc
    37981 aagtcaagga actgcatgcc tcgtcctaca taacgtttag cgatacttac tactaaacga
    38041 aggtttgctt ctgccagacg ctgtttcgct tcttggtcgc cttcttcgat tttcaatgct
    38101 aacgctactt cttcttccgc agttaaaagg gagacacggc caatttcttt cagatacata
    38161 cgaaccgggt cattgatttt tacaccggtt ggcgcagaaa gatcttctgt ttttgcttgt
    38221 tcagcttttt tctcagcact cttcaagctg tgaattgatg gatcaccatt ttcatccacc
    38281 acactgatac ctgcgtcttc aatcttttgg atcaatttgt ccatttcatc tgcattcaaa
    38341 gtgaatggtg tagcaagctg attggataaa tcatcatata caacttgtcg tttgggtttg
    38401 ttttctttga tgaaagctgc tactgctgct tcatacgttt ttttatctgt tccatttgcc
    38461 ataaaagaag gccccccttg agtagctatt tggcttgttt taactgtttc gttaaattga
    38521 tgatttcaat cgtcaattca agctcaagct gtttgtttcc agttcgtctg gcttcttgct
    38581 gcatttgttt ttttaatgtt atttcatcag ctaagccaga tttgctgatc acatctagca
    38641 agtcttggat ttctcgtggc gaactttctt ctgccaccgg taaatattcg atttccacca
    38701 ccaaccgcct taaattttca tcttgcaaaa agtctaaaaa ctctgcaagg ttgaattcat
    38761 aatgttgtga gacatatgca tcgaataaaa aataaacttc tgcatatgca tcatgaataa
    38821 actgaaattc tcgttctttt aaaatattac ggacagaaac ttctttaaat gcacgatata
    38881 atagaatctg ctctgccttt tctacttgcg tcaaaggtct ctttttccgt gcagtttctt
    38941 gtctcgtaac ctgagtgtgt tgactttcct gaggttcttg acgattttgc tgatttgtcc
    39001 ttcgaagatc gcgcagttgt ttttgtatcg tctctctgcc gatctgaaac tctgttgaca
    39061 gttgagatag atacatatct tgttcgattg gtgaaacaac tcggctaagt tcaactagta
    39121 attcttcgag gtattcgatc ttgtcttttt cattatctaa atttttccct tgttttaaat
    39181 aacgtgattt aaaagtaaag actgtttcgc gcccatgagc cactaattct tggaaagatg
    39241 cttctccata cttacggaga tattcatcag gatcaagctt ttctggaacg actacgacag
    39301 aaagtgctaa tcgactgttt tgccgaagca attcaatccc tcgattggtt gcttcaaaac
    39361 ctgcgttgtc tccgtcatag caaaagacca attctttcgt cagcttttcc atttgttgga
    39421 tctgttgagc tgttagactt gtccccatag aagctaatcc agttttgata ccagcctgcc
    39481 atgcagcaat cacatccata aacccttcga acaacaaaat ttctccgttt ttccggatat
    39541 cgcttcttgc tttatcaaga ttaaataaaa cttgtctttt attgaacagt tctgtttctg
    39601 gactattcaa gtatttgggc tgatcttttt cctgcccttt ttcaggtgca agccagcgtc
    39661 ctgaaaaacc gatcgtctgg ccttgagaat tacgaagtgg aaacatcact ctttgataaa
    39721 aacgatcaga catacggcca tcatcatgcg tgacaaacaa tccagagcgt tctaacaatt
    39781 catttgaaat tgattcattc ttgaatactt gctgaagaaa agaacgctca ttaggtgcaa
    39841 aacctatgtt gaattcttca attaattcta aagacaaccc acgactttgc aaatattcta
    39901 aagctggctg tccagctttt gtatgcaata gcatgtgatg atagatttcc gctgcttttt
    39961 catgagcagc aatcagtttc cccgtagcag attcaggatt ttggacagct atagtacgct
    40021 gacgccattg atcttctaac ggtatctgtt cgaagtctgc cacttttacc actgcttctg
    40081 gaaaacttag accttctagt tcttgaagaa aagtaaacac gtttccgcct ttaccacaac
    40141 caaagcaatg aaaaatctgc ttgtcttctg caacagaaaa agacggtgta cgttcttcat
    40201 gaaaaggaca cagccccaag taatttttac cagacttttt caactggaca tattgtccaa
    40261 tcacctcaac gatattcgtt tgactgcgta ctgtttcgat aacctcttga ggaatccgtt
    40321 gagccatttt ttcaccccct gctctattga tatcttttgg gtttaacaga agatatttca
    40381 ggagaataac cggtaaaaaa tcgcactaac tgaaatcagt gcgattttaa aggacacaat
    40441 tatacatgtt tgattgtatc atatttcatt gctttttcaa gtataacaca tttctatctc
    40501 attaagcatc aatttgctac tcttttcagt aactgatttt gaatatcagc cttccagtac
    40561 aatactcgaa gaacaatatt tacgagcatg aatacaaaga agtatcctac gctctctccc
    40621 aaccctaagt ttacttccaa aattccatgg aacagcattg cagctacata tatccctacg
    40681 atcgtcgtta ctgctaggaa gatgcgcaat gggttgaagg gataacaagc ttttacaact
    40741 gccatgcaac tgatcccaat cagcaagtaa tacatcaaag tagtagtttc agcatttccc
    40801 catgcgaatg tttttccaag tagatacacc gcaatgatat tcactactac aagcaaggca
    40861 tttggtaaag cattaataag tgctgtaggc aaataacggt aagtgatttt tcttttatct
    40921 tgttcaaaag ataaaaagaa ggaaggatac ccttcaatcg ctaaatcaat cagcgtgatt
    40981 tggataggga taaacggaaa tccaattgct gtaacggcac atataatcga taaaataaat
    41041 gaataaatcg ttttgataaa aaagatgcct gagactttcg tcacattgtt gaccactcgg
    41101 cgtccttcaa acaggacttc tggcaatgta gtaaaatcag agtccaacaa taccagattc
    41161 gcaatctgac gtgttgctcc atcaccttct gccatagcaa tgctgcaatc tgcttctctt
    41221 aacgccaaaa catcattcac accgtcgcct gtcattgcaa ccgttctgcc gctttctttc
    41281 aattcattta ccagcaattt cttttgctgc ggactcactc gtccaaaaac cgtatagcga
    41341 tgagcagctt ctcgcacttc catttcttca gtaatttgag aaagatcgac atacgcatca
    41401 taattcggta atcctgcacg tctagcgata ttggagacag taaaaggatt gtctccagaa
    41461 atcactttca gatccacacc ttcctcatga agataagcta gtgtttcttg agcgtttttt
    41521 cggattggat catcgatttc aaacagagca accggctcaa caaatgctgg tttttcttcg
    41581 ttcaattgct gatttggagc gatccccaac ataagtacgc gatagccatt cgcttgggct
    41641 gtttttagac tgtccggaag ccgatcttct tccaccaaac gttcaggcgc tcctacgtac
    41701 acgactccca gttctggaaa ttccatcgcg ccccatttgc gatcagaaga aaatgcgact
    41761 ttgtttgtca catcataccg attagaaagc tcaaaataat ctctcatagc ccgcatagtg
    41821 atgttgttat cactgctttc tgccagatac gtccccataa tttctgggat ggctttctcg
    41881 tacagcggat gtagtgcttc tacccgctgg atactcattt tcccttcagt aatggtccct
    41941 gttttatcca agcataatgt atcgacatgg gccagcgttt caattgaata catgtcttga
    42001 acaaggattc gtttttttgc cagtttggtt acccctgtcg ttaatgcaat acttattagc
    42061 aataccaaac cctttggaag catgcctaac aaagctgctg cagatgcaat aacagaaatt
    42121 ttgatagaag catcgcggat gaacagtgct tccaaaaata gtatgatccc caaaggaata
    42181 atcacgaaac tagtgaattt ggatactttg cgaatcgaat ctacaagttc agaacgaata
    42241 gctttgtgag tctttgcctc gctagtcaag cggacagcat aattatctgc acctactcgg
    42301 ataacttcag ccatgacaga accgcttgtc aaaaagcttc cagaaagcaa ttcagctcca
    42361 gtttctttga caatcaaatc agattcgcct gtcaaaagag cttcatttgc ttctgcccgt
    42421 ccagatataa cctgtaaatc ggctggtatc tgttctcctg ctccgatttt taccaaatct
    42481 cctaatacca gctctttagt agaaatcgat tgttctttac cgtcccggat cactagtact
    42541 tctgattggg aaacaatcga taatttccgt acaagatttc ttgcatgtat ttcttgataa
    42601 attccaataa cgatattgac gacaataatt gctaaatacg ccatgttgga atacgcacct
    42661 acaaataaca gacaaatccc aataccaaag ttcagaaaat tgaataacgt caatatatta
    42721 tcgctgaata tttgttttgt actttttgat gcatcttcct ggtagtcatt ttgctggcca
    42781 gaagccattt tctgaactac ctcttgctgt gtaagtccat tcattttctt tttctccatt
    42841 ttccgccaac tttcaaaaat aatcacgtgt ttaagtctag cgtgttccaa gattaaaaac
    42901 aacttgtttt tataaaattt atagaaaaac acacgaatga ttttacaaaa aagatgccaa
    42961 cctttttgtt tgaatcaccc gtgtgctgtt tttattatca atctatcttt acttatattg
    43021 ttcctccaaa cggctcatcc accaacctag tgctgtaccg gctataaaaa gaacaggaca
    43081 agctagtacc ccagcaactc ccaatcccgc gtaatcggta ggaatgatca taatcgttga
    43141 agcaaaaact acacctaaaa tgaaatggaa tagcttgcta tatgcctttg agaaaatgta
    43201 atccatgatt tttgacaatc caagaacaca aagaatccca ccaattccga tcggcacaat
    43261 cacttcaagt cgtacttctt taaatccgtc agccatcgct ttatacaatc ccatatacac
    43321 caaaaaatta gaagggctca atcctgggac gatcataccc aaaccaatca atccgccggc
    43381 aatcagccat gttccaaaat tctgatcgac ttgcccgtca aataattgag cgccgtataa
    43441 taaaaacaaa taagccagaa caaaagtaac aaccatgatg atcacatcag aagtatctct
    43501 tccttttttc cctgcttctt tccataacgc aggaacagtt ccagcgatac atccgacaaa
    43561 aaaccataat atcgttgttt catacgcacc taataaaaaa ctgactaaaa aagaaagtag
    43621 aaagatgcct gtcactccgc ctagaccaac aggcagaaaa aacaaaacat tttctttgaa
    43681 attttttgta aggtgggcta aaaaagaaat cagccgctca tacatgccaa agacagctgc
    43741 taatgcacca ccgctgactc ctggcaagat aaatcctgaa ccgataaaca ttcccttcac
    43801 aaaccgtaac atccagtctt tggttgtgta ccccacttct tgattttcca ttttttgcct
    43861 tttttccact atttgcgtac tccttctttc atttaatttt attcatgatt gaacatttcc
    43921 tgctatttta cttaaagact ttttcatagg tctctattat tttttcattc attttgacaa
    43981 tatcaggata aaacatgtta cctccattat gatagagata gccgccattg taaagcaagg
    44041 actggatccg ccaatatcgg tattgtttcc cttcctcatt tcctaaaagt gggctcagca
    44101 catctttaga atacgcttct gctaattcta ctgtgttttc tccgccatga ttttttacat
    44161 aggcaatata atctttccca aaattatatg cttggacagc tgtccataaa tcgcatcctt
    44221 gtttctttgc ttctactatc atttccgcca aatatttgac accttgatca atactgtcag
    44281 ctggattatc aatgatctca gcttctccat gcagactttc cgagctctgc atgagatctg
    44341 tcccttttcc ttttgattcg gtcaatatta tcgccttgat caaattctcg tagttttcca
    44401 tatgataatt ttttgcagct gttgttattt ctatttgata gctttccact cgttttaccg
    44461 aacgataagt tcttcctcat aaaaacaaca ccgcaccaat aaccgtcaga atgatcatgt
    44521 aaaaaagaat acggataata gataatcgtt ttcgcttcat ttcatcacct agttattttg
    44581 acatcttttt ttatcttact atttctttat cttcttggct atttcttcct tgatttctgt
    44641 gtacgcctta agaaaaaatg aattttcctg tattttttga catcttacga aaatgatcgt
    44701 tgtttttcag taaaaacaat tgcccaatga ctgatataat cgttatactt tacacagtag
    44761 gtgattatgt gaaaaaagaa aagaaatatg caaaaatgcc agatgggagc gaaatttact
    44821 atgaaaagag cggtcaaggc ttccctctct ttttactgca tggcaatgac ggcagcggcc
    44881 gctttttttc agaacaggtc cctgtactcg aacgctacta taccgtgtat ctagtagata
    44941 gccgaggaca tggtcgttcg acaaacgaag catctatgtt gaattttcag ttaatggccg
    45001 aggatctgaa tacgatcatg ttgctggaaa aaatcgatca ggcagatttt cttggattta
    45061 gtgatggagc aaatctagca ctggtatttg ccagcagctt ccctaaaaaa gttcatcgat
    45121 tgattttaaa ttctggaaac acgcttgtta aaggcgttcg tttttctgcc agagtcatca
    45181 gtaatattca ttatgcatgg gtatggttac tctctttatt tcgcccaagt cttcgcaaaa
    45241 atcttttagt tatcaaactt ttgctgcatg acatcgggct tacagaaaat gatttaaaaa
    45301 aaatcaactc ccccactttg atcatcgtgg gaaaaaaaga tgtaatcaag ctaaaacatt
    45361 ctttgtatat agctaaaact ataccaaaag cttcctttgt gcttgtgaaa gaacaaggac
    45421 atgaattagc tagaaaagat cctgaacgct tcaatcgaga agttcttcaa tttctttctg
    45481 aaacataact attaggaata gaaggtgatt tatttgttaa aaaataccat acaatggctg
    45541 aaaaaccacc taggactgtt caaaacaatc tttttgattt ctgtcattgt cattattgtc
    45601 ggtgaattga tgtccatcgg taaaactcta tctatccagc aattaggaga aacgtttgcc
    45661 actatacctg tatggaaaag cggactaatg ctattgattg gtcttatctc tgtgttacct
    45721 atggttggtt atgatattat tttgaatcgg atgcttgaac aaaagcagaa tcctcgttac
    45781 ctgtttgaga caagctggct gatcaatacg atcaacaata ttgccggttt cggcgggttt
    45841 gttagtgtgg ggcttcgctc tgaactatat ggacaaaaga aagagagcag aaacgtgatc
    45901 caagcattat cgaaagtttt cttgtttttg atggctggac tttctattta cagtctgctt
    45961 tcttttttac ttgtagtctt cacgccagtt gctccttttt tgaagcaata ttggatatgg
    46021 ttgatcggcg gcggtctata ttttcctgtc gtctttttat ttactacttt caaaaagaaa
    46081 ggattccttg gtggtacctc acttaaaact caaggacagc tattgctagt ttcattgttg
    46141 gaatggaccg gtgtcctgtt tagtttcctt tctgttggtt atttaatgga aatccggatt
    46201 gatctgtggc aaacaattcc attattcatt gctgcttcag tgataggaat cgtgtctatg
    46261 attccaggag aaattgggag tttcgatgta atgatgatta tcgggttgtc cgctattggt
    46321 gtgccaagag aaatagtcgt tgtgtggatc ttgctctatc gcctgttcta ctacatcatt
    46381 ccgtttttga ttggaatcgt ctttttcttc aaaaatatcg gttccacttt tgatcagcgt
    46441 tattctggta tccctaaaca attagcaaca gaaattgcgc ataaaatcgt ggttgtctta
    46501 ttatactttt ctggaatcat gctagtactt tctgccacta tcccgcaagc ttttacagag
    46561 tttcgctggc ttcatagttt gaacccattg aaatttcatt ttattatcca atttcctagt
    46621 atactattgg gatttctttt aatcattatg ggcagaggga tcgcagcacg tgtgaaacga
    46681 gcatatttgc ctacaatctt cttgattgtt ctagctttac tttatgtttt attaagtgat
    46741 tttagtttca caccggtggt tttcttgtct attctgcttc tgatcattct tgcttctaaa
    46801 aacgaattat ttcgggaaca attgatctat tcatgggagt ggcgtacgat cgatggaatc
    46861 attattggtg cactgtcctt gctttacatc gttattgggg tatacaatct gccggatttc
    46921 cctcatcggc gtcaccattt tatttccttt ttcttatttc cttctgaaaa gatttggttt
    46981 tcgggattgt tggccatcat cgctgttagt ttcatgatcg ttctatttgt tcatttcctt
    47041 caaggagaaa agaaacagat tggcgaagcc tttaatgaag aaaaggccct gaagatcctt
    47101 acaacttatg gaggaaactc cgatagtcaa ttgatcttct taaaagataa gcggatgttt
    47161 gcctatgaaa aagacggtga accaactgtt cttctccaat tcgcttgctt caataataaa
    47221 tgtattgtaa tgggtgatcc ttctggtaag aaagaagatt ttccagaagc aatcgaagct
    47281 tttatcgaag aaacagatcg actatgctat ttaccagttt tctacgaaac aagcgaagag
    47341 attgttatga ttttgcatga attcggctac gatttcatta agatggggga agaagcttat
    47401 gtagacctga atagttttac aacttcaggg aaaaaaatga agggcactcg tgctgtcttg
    47461 aatcggatcg aacgagaagg atttacattt gatgtcttac aaccaccatt ttctactgag
    47521 caaatgagta ctttcaaaaa tatctctgat aattggttgg gttcacgaaa ggaaaaaggg
    47581 ttttctttag gctttttctc tgaagattat ctacaaagag cgcctattgc agttgtcaaa
    47641 aatacccaag aagaaattgt cgcttttgca acgatcatgc ctacttacac gaacaataaa
    47701 gtaggaacga ttgatctgat gcgatatgat tctaaaaaag caccttctgg cagtatggat
    47761 ttcttgttct tgcatttgtt tgcctatatg aaagaagaac atatccaatg gttcaattta
    47821 gggatggccc ctctttcaaa tgtcggaact tccagaaaga gttttttaca ggaaaggatt
    47881 gcatacttgg tttacgagtt tggttctcat ttttattctt tccatggatt aagagaatat
    47941 aaaagcaagt acgctacaaa ctgggtctca cgttatactc tatattctag agatagttgg
    48001 attgcatatg ttatgattgc tttgctgatt attgataatg cgccagttga gcagacacaa
    48061 ggtagaataa gtggtttgaa aaaatggata cgaagaaagt attaaaaact gcataccgat
    48121 agatgaacac tctcatgatg aataaaagag actgaagtaa aacgaaagtt catgcttcta
    48181 caatctcttc ttatacaatt gaagaacaaa gagcggtcca aacagaaatc ctgtttggac
    48241 cgctctttta ttttaaacat ttttcattac caaaatattc gagtcgcgtt tcttatgctt
    48301 cgatctctgt aacgatacct gaacctactg tccgtccgcc ttcacgaatt gagaatgtcg
    48361 ttccgttttc aacagcgatt ggatggatca agtcaacatc aatcgtcacg ttgtcaccag
    48421 gcatgaccat ttctgtatct tctggcaatg tgattgttcc tgtaacatca gttgtgcgga
    48481 agtagaactg tggacgatag ttgttgaaga atggagtatg acgtccacct tcttcttttg
    48541 tcaacacgta cacttctgct ttgaattttg tatgtggagt aatggaacct ggtttagcaa
    48601 gtacttgtcc acgttcgata tcgtcacgtt ggatcccccg taacaacacg cctacgttat
    48661 ccccagcttc cccgtaatct aacgttttac ggaacatttc tacacctgtt acaactgctt
    48721 tttgtgtttc aggtttgatc cctacgatct ctacctcatc accgacacga acagcaccac
    48781 gatcaatacg acctgaggca acagttcctc gacctgtaat tgagaagacg tcttccactg
    48841 gcaatagtaa tggtttatct gtatcacgtt ctggtgttgg gatatattca tctactgtat
    48901 ccatcaattc cataatagca gcttcagcat ctggatcgcc ttgcaatgct tttaatgcgg
    48961 aacctttgat cacaggagtg tcatcgcctg ggaaaccata ttcgctcaat aactcgcgaa
    49021 cttccatttc taccaaatcg atcaattctt catcatcgac taaatcaact ttgttcaaga
    49081 aaacaatcag gtattttaca ccaacttggc gtgacaataa aatgtgttca cgtgtttgag
    49141 gcatcggacc gtcagttgca gaaacaacta agattgcacc atccatttgt gctgcccctg
    49201 taatcatgtt tttcacatag tccgcgtgtc ctggtgcatc aatatgcgcg tagtggcgtt
    49261 tttctgtttc atattctacg tgtgcggtat tgatcgtaat cccacgttca cgttcttctg
    49321 gagctgcatc aatactagca tagtcttgag gatttgctag gccttttttg cctaatactg
    49381 tagtgatcgc agcagttaaa gtagttttcc catggtcaac gtgtccaatc gtaccaatgt
    49441 taacgtgtgg tttacttctg tcataatgtt cttttgccat aatttacaaa cctcctaaat
    49501 aatatagatc actgaaaaat ttcagctctc acctttttat tatagtcttt ttttgactaa
    49561 atcaaaaaga atacgtccga cttacttttt gtaagattcg aactaagctt attatacgtc
    49621 aatattggtc aaagtcaact aagagcactc gtctgtctta tacgcgaaaa aagtaccttt
    49681 tataaataaa aaaatgtgtt ttttgagaaa aacttcaaga tagtccttaa ctttcttttg
    49741 aagcaataag aacaaaatga ggaaaagttt aaaattcagc tgttgccaac aactttcaat
    49801 agacgaaatc ttctatttcg ttaataataa ggaaagaggt gaagtacatg aaaaaattaa
    49861 ttggcgtact tgtcgtttta ggcatatttc tcggcggaag ttttgcagct tatcattatt
    49921 tttacggcgg cgaagcttat tatacaaaaa tcacgacgaa tggcgaaatg tcagctaccc
    49981 aagcagataa cggagagaaa ttcacgactt atacctacaa gcaagcggct tataataaag
    50041 atggtgaaaa aaaacaggtg acgcttcgcg aagaaagaga acatccatta aagatgaatg
    50101 cgtatcttaa actcaaagtc aatcctcgta aaggtgtatt aagctgggaa gaaataaagg
    50161 cttctgaagt gcctaaaaat gcggcagaaa aaatcgataa ttagtaaatg gtcttaaaat
    50221 tttattagat aaaaagaggc tgggacagaa gcgtttaact ccaagaaata agaaggagtt
    50281 gcttctgctt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt
    50341 gtctcacgct ctttttttgt tatattcgtc ttttccgatc cttctcttga cacaattcat
    50401 ttaactgatc actggtactt gaacacctaa tattctagtc atttttataa acgattcaat
    50461 acttcatcca catttttgat catcacggat atcgtaccat ccggattatt cacaaattca
    50521 atcaaatctg ggttgcgata tacatctact ggtacgatca gttcgatccc gtttgatagc
    50581 ttgaattttt gttttccaaa ttttttctca gaaatttctt gtacttcttt tacaggaggt
    50641 gcttctggga caaatccttg ctcttgtact tcttctttaa aagccatttt tgccgtgatg
    50701 ttttccttga agactttttc cgcaatcatc tcatgatcca accgaccttt ttcttctatc
    50761 gtatcataga cagcttcttt caaatcagcc atgatatcaa attcttctgt ctcaaatttt
    50821 tttccaagat gcttggctac tttttggatc acttttacat tttcttctaa agaaggtgca
    50881 ggaacacttt cgatcactct tccagaaaaa taaagctctt ttttgccaga aaactcatat
    50941 tttttttcaa ttaattcgta ggtccaatca gtcaaattca cagcgaatgc ttcatctgct
    51001 ttttgtgttt tcgcccctag aattgctcga ttcaaaatca agcggttgtc gatccctgct
    51061 tcgtctgctt caacaaagtg agtgtaagcc tcacgataat tgactttcaa taaagataag
    51121 tacatcactg tatctaattc atacaagaca acaaatacat ctgcactagg tgcttcttca
    51181 ctttcttggt agatatcgaa ccaccgctga acgattgctt cacttgcttc cacaaaattt
    51241 tcttgagcga ttttaagtga ttcaacgatc atgctttctt ctgctagttg tccagttttt
    51301 gtttgagcag aagagatttt ttggatcttc ttttgaagaa aatcacgaat atattctttt
    51361 gtcagatcca gctctttttg tgaaaagact ggagaaccag tttctctgtc aataatatgc
    51421 agaattgctt tttttaaata gatatccatt ttctcattcc cttctgtctt agtttacgga
    51481 aaaaaaatca aaaataccag aatcaaaatc attttctttt aaacaaaaat ttcaagagta
    51541 gaaacaaaat ggattctttt tttcgaaaaa tgcgctacac ttatagttga acgaataaaa
    51601 aaggagctga aagtatgaat tcattaacaa gcacttatcg tctatcaaat ggttacgaaa
    51661 tcccggtagt aggattcggc acatggcaaa cacctgatgg ggatgtcgct gtttcctctg
    51721 ttaaagaagc gctggctgca ggttatcgcc atatcgatac tgcccaagga tacaagaatg
    51781 aagaaagcgt cggccaagcg attaaggaaa gtggaattcc tagagaagag atattcttga
    51841 caacaaaatt atggaatgca aaccacagct atgaacttgt gatgtcttct tttgaagaat
    51901 cattgaagaa gctccaaaca gattatttag atctgttttt gatccactgg ccaaaccctg
    51961 ttgcttttcg tgataattgg gaacaagcaa atgcagatac atggcgagca tttgaagaac
    52021 tgtatgaagc aggtaaaatc aaagcaatcg gtgtgagcaa tttcttaccc caccatctag
    52081 atacactagc aaaaacagca aaaattatgc cgatggtcaa tcaagttttc cttgcaccag
    52141 gcgaattaca gacaactgta gttgagtatg caaaaaaaca tgatatgatc ctcgaagcat
    52201 atagtccatt aggcactggt aaaatcttcg atgtgcctga aatgaaacaa atcgctgaag
    52261 cacatgacaa gacgattgca caagttgctt tgcgttggtc gctgcaacac gaattccttc
    52321 ctcttcctaa gtcagtgaca ccaagcagaa tcaaagaaaa tactgaatta tttgattttg
    52381 aattaacaga agaagaaatg aaacaaatcg atcagttaga tggtgtagtc ggcaaagcaa
    52441 aagacccaga tactacccaa ttttaagtcg aaaggtgatt ttatggatcc aatcgaacgc
    52501 ttgaattctc tctcggaaga agttacccag acttttcatt ccgattttgt ctttttgatc
    52561 gatgcagaga aaatccaaca ttttcctgca agaaactgga cacatgatca aatcatcgaa
    52621 gaactgaaaa aacggttcga tcattcgcta atggtcaaac cctggcacga acatgaagtc
    52681 atttattcac ctgaactacc tgtttttgcc ttgataccga aaaaatagaa agaatggaac
    52741 ggattatgta tatatacata atccgttcca ttttttattt ttctttcaaa aaacgggaaa
    52801 atacgcaaat ttttctgttg taatttgcgt atacgcaagt ttttttgcat tactcctttt
    52861 catttggctg tttttttctt ataatcgaaa gggtatgagc gaaggagaga acatatgaag
    52921 aaaaaacaga aacaaaaatt attaaatcaa ttccgtcctt cattggatgg tgtaagagga
    52981 caattattcc gttctctaga agaagagagc ttgcgacgtt atggtttgcc gttacaagtc
    53041 atgcttgatc ccaaaaaccg tcaaatctta acgatcggat tagctgggaa aacaaatgaa
    53101 gatacacgga tcaacgtgcc aatcgatgac aacttcaata ctgtattaaa gagaattcgt
    53161 tcaggagaaa acgggatttt cgaacgattc cgtgacaatc ttcttattga aatcgtgtct
    53221 tactggaatg atcaacgatt gaaaaacgat gaaccaactg cgcaagctgt tgcgacacca
    53281 gttacagaag aaacagtcag tgtgccagaa gaaaaaacgg aaaaaccaaa agcggacaca
    53341 actgaaaata cacgctcaac tgatacgaat gccgctttaa gttttgctga tttttcaaaa
    53401 gccgttgctg agtatcctaa attttacaca gaacaaaatg aacatgaagt agttattaat
    53461 gaaaaaaatg ctgatgaacc tcgtaaatta gcaactattt ctatgactac tgaaaatgag
    53521 ttcgttatcg aaaaagcact cgaacggaaa tacaaagtca aattgacttt gattcctttg
    53581 atcgaacaat ttgcagcgac agcgattact gatcgataaa aattctattt gtcataaaag
    53641 gctgggattt aaaatcccag cctttttagt catataataa tttctacagc aagtaaaaag
    53701 aaattccttc taacatcagg ctcaaatatc agcttgacct gtccaacaaa tcacctatga
    53761 tcctacaatg ttaggcgact tctcttctac ctgaagatat cttggagttg cctgatttct
    53821 taagatgcct aaccttaact ataaaaaatt ctaataataa acatattttc tgatagagaa
    53881 aaaa
//
LOCUS       DACCXM010000007        52338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCXM010000007 DACCXM010000000
VERSION     DACCXM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.007"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(122..499)
                     /locus_tag="IVY61_000334"
     CDS             complement(122..499)
                     /locus_tag="IVY61_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969475.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /locus_tag="IVY61_000335"
     CDS             755..1483
                     /locus_tag="IVY61_000335"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP7969476.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="IVY61_000336"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="IVY61_000336"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP7969477.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="IVY61_000337"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="IVY61_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP7969478.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="IVY61_000338"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="IVY61_000338"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP7969479.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /locus_tag="IVY61_000339"
     CDS             4618..6057
                     /locus_tag="IVY61_000339"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP7969480.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6089..7132
                     /locus_tag="IVY61_000340"
     CDS             6089..7132
                     /locus_tag="IVY61_000340"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP7969481.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="IVY61_000341"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="IVY61_000341"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP7969482.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="IVY61_000342"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="IVY61_000342"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP7969483.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="IVY61_000343"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="IVY61_000343"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP7969484.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="IVY61_000344"
     CDS             10651..11565
                     /locus_tag="IVY61_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP7969485.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="IVY61_000345"
     CDS             complement(11698..13263)
                     /locus_tag="IVY61_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7969486.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="IVY61_000346"
     CDS             complement(13256..14707)
                     /locus_tag="IVY61_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7969487.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="IVY61_000347"
     CDS             complement(14721..15737)
                     /locus_tag="IVY61_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7969488.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /locus_tag="IVY61_000348"
     CDS             15957..17045
                     /locus_tag="IVY61_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7969489.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /locus_tag="IVY61_000349"
     CDS             17099..18625
                     /locus_tag="IVY61_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP7969490.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /locus_tag="IVY61_000350"
     CDS             18627..19832
                     /locus_tag="IVY61_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7969491.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="IVY61_000351"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="IVY61_000351"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP7969492.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..22754
                     /locus_tag="IVY61_000352"
     CDS             21330..22754
                     /locus_tag="IVY61_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7969493.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23215..24324
                     /gene="pdhA"
                     /locus_tag="IVY61_000353"
     CDS             23215..24324
                     /gene="pdhA"
                     /locus_tag="IVY61_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP7969494.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24327..25304
                     /locus_tag="IVY61_000354"
     CDS             24327..25304
                     /locus_tag="IVY61_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP7969495.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25329..26972
                     /locus_tag="IVY61_000355"
     CDS             25329..26972
                     /locus_tag="IVY61_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP7969496.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26980..28386
                     /gene="lpdA"
                     /locus_tag="IVY61_000356"
     CDS             26980..28386
                     /gene="lpdA"
                     /locus_tag="IVY61_000356"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP7969497.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28548..28823
                     /locus_tag="IVY61_000357"
     CDS             28548..28823
                     /locus_tag="IVY61_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP7969498.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28829..29596
                     /locus_tag="IVY61_000358"
     CDS             28829..29596
                     /locus_tag="IVY61_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP7969499.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29764..31593
                     /gene="typA"
                     /locus_tag="IVY61_000359"
     CDS             29764..31593
                     /gene="typA"
                     /locus_tag="IVY61_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP7969500.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31854..33626
                     /gene="recQ"
                     /locus_tag="IVY61_000360"
     CDS             31854..33626
                     /gene="recQ"
                     /locus_tag="IVY61_000360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP7969501.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33740..34042
                     /locus_tag="IVY61_000361"
     CDS             33740..34042
                     /locus_tag="IVY61_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP7969502.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34045..35208
                     /locus_tag="IVY61_000362"
     CDS             34045..35208
                     /locus_tag="IVY61_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7969503.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35234..38662
                     /locus_tag="IVY61_000363"
     CDS             35234..38662
                     /locus_tag="IVY61_000363"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP7969504.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38683..39807
                     /locus_tag="IVY61_000364"
     CDS             38683..39807
                     /locus_tag="IVY61_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969505.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39797..40234
                     /locus_tag="IVY61_000365"
     CDS             39797..40234
                     /locus_tag="IVY61_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7969506.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40218..40556
                     /locus_tag="IVY61_000366"
     CDS             40218..40556
                     /locus_tag="IVY61_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP7969507.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40675..41235
                     /gene="rsmD"
                     /locus_tag="IVY61_000367"
     CDS             40675..41235
                     /gene="rsmD"
                     /locus_tag="IVY61_000367"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP7969508.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41232..41723
                     /gene="coaD"
                     /locus_tag="IVY61_000368"
     CDS             41232..41723
                     /gene="coaD"
                     /locus_tag="IVY61_000368"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP7969509.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41713..42756
                     /locus_tag="IVY61_000369"
     CDS             41713..42756
                     /locus_tag="IVY61_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7969510.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42915..43643
                     /locus_tag="IVY61_000370"
     CDS             42915..43643
                     /locus_tag="IVY61_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP7969511.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43759..44004
                     /locus_tag="IVY61_000371"
     CDS             43759..44004
                     /locus_tag="IVY61_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7969512.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44001..44219
                     /locus_tag="IVY61_000372"
     CDS             44001..44219
                     /locus_tag="IVY61_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP7969513.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44355..44894
                     /locus_tag="IVY61_000373"
     CDS             44355..44894
                     /locus_tag="IVY61_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP7969514.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44887..47160
                     /locus_tag="IVY61_000374"
     CDS             44887..47160
                     /locus_tag="IVY61_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP7969515.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47198..48244
                     /gene="holA"
                     /locus_tag="IVY61_000375"
     CDS             47198..48244
                     /gene="holA"
                     /locus_tag="IVY61_000375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP7969516.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48333..48587)
                     /locus_tag="IVY61_000376"
     CDS             complement(48333..48587)
                     /locus_tag="IVY61_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP7969517.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48987..49256)
                     /locus_tag="IVY61_000377"
     CDS             complement(48987..49256)
                     /locus_tag="IVY61_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969518.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49726..50181)
                     /locus_tag="IVY61_000378"
     CDS             complement(49726..50181)
                     /locus_tag="IVY61_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP7969519.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50319..52247)
                     /locus_tag="IVY61_000379"
     CDS             complement(50319..52247)
                     /locus_tag="IVY61_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7969520.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        17190 a         9005 c        11415 g        14728 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    22801 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    22861 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    22921 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    22981 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    23041 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    23101 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    23161 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    23221 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    23281 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    23341 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    23401 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    23461 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    23521 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    23581 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    23641 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    23701 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    23761 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    23821 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    23881 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    23941 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    24001 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    24061 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    24121 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    24181 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    24241 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    24301 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    24361 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    24421 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    24481 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    24541 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    24601 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    24661 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    24721 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    24781 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    24841 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    24901 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    24961 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    25021 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    25081 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    25141 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    25201 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    25261 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    25321 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    25381 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    25441 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    25501 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    25561 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    25621 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    25681 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    25741 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    25801 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    25861 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    25921 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    25981 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    26041 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    26101 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    26161 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    26221 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    26281 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    26341 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    26401 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    26461 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    26521 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    26581 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    26641 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    26701 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    26761 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    26821 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    26881 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    26941 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    27001 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    27061 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    27121 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    27181 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    27241 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    27301 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    27361 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    27421 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    27481 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    27541 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    27601 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    27661 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    27721 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    27781 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    27841 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    27901 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    27961 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    28021 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    28081 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    28141 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    28201 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    28261 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    28321 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    28381 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    28441 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    28501 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    28561 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    28621 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    28681 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    28741 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    28801 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    28861 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    28921 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    28981 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    29041 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    29101 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    29161 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    29221 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    29281 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    29341 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    29401 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    29461 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    29521 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    29581 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    29641 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    29701 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    29761 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    29821 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    29881 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    29941 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    30001 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    30061 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    30121 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    30181 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    30241 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    30301 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    30361 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    30421 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    30481 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    30541 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    30601 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    30661 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    30721 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    30781 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    30841 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    30901 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    30961 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    31021 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    31081 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    31141 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    31201 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    31261 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    31321 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    31381 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    31441 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    31501 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    31561 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    31621 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    31681 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa
    31741 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    31801 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    31861 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    31921 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    31981 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    32041 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    32101 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    32161 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    32221 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    32281 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    32341 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    32401 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    32461 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    32521 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    32581 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    32641 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    32701 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    32761 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    32821 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    32881 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    32941 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    33001 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    33061 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    33121 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    33181 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    33241 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    33301 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    33361 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    33421 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    33481 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    33541 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    33601 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    33661 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    33721 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    33781 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    33841 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    33901 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    33961 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    34021 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    34081 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    34141 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    34201 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    34261 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    34321 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    34381 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    34441 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    34501 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    34561 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    34621 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    34681 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    34741 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    34801 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    34861 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    34921 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    34981 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    35041 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    35101 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    35161 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    35221 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    35281 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    35341 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    35401 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    35461 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    35521 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    35581 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    35641 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    35701 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    35761 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    35821 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    35881 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    35941 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    36001 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    36061 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    36121 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    36181 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    36241 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    36301 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    36361 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    36421 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    36481 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    36541 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    36601 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    36661 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    36721 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    36781 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    36841 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    36901 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    36961 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    37021 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    37081 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    37141 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    37201 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    37261 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    37321 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    37381 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    37441 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    37501 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    37561 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    37621 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    37681 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    37741 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    37801 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    37861 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    37921 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    37981 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    38041 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    38101 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    38161 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    38221 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    38281 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    38341 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    38401 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    38461 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    38521 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    38581 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    38641 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    38701 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    38761 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    38821 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    38881 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    38941 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    39001 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    39061 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    39121 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    39181 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    39241 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    39301 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    39361 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    39421 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    39481 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    39541 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    39601 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    39661 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    39721 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    39781 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    39841 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    39901 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    39961 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    40021 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    40081 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    40141 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    40201 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    40261 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    40321 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    40381 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    40441 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    40501 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    40561 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    40621 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    40681 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    40741 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    40801 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    40861 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    40921 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    40981 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    41041 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    41101 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    41161 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    41221 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    41281 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    41341 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    41401 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    41461 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    41521 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    41581 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    41641 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    41701 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    41761 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    41821 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    41881 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    41941 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    42001 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    42061 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    42121 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    42181 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    42241 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    42301 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    42361 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    42421 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    42481 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    42541 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    42601 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    42661 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    42721 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg
    42781 tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa
    42841 acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt
    42901 ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa
    42961 gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga
    43021 tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat
    43081 tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg
    43141 gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt
    43201 acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac
    43261 gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa
    43321 tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag
    43381 agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt
    43441 ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg
    43501 ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc
    43561 agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa
    43621 aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga
    43681 cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat
    43741 ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat
    43801 gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat
    43861 aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa
    43921 gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg
    43981 taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg
    44041 ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt
    44101 tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat
    44161 cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat
    44221 ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg
    44281 ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg
    44341 gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag
    44401 ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag
    44461 tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag
    44521 aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag
    44581 atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg
    44641 cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac
    44701 attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt
    44761 atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat
    44821 ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag
    44881 gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt
    44941 acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt
    45001 gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt
    45061 ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa
    45121 aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt
    45181 ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta
    45241 aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact
    45301 gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa
    45361 gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc
    45421 accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt
    45481 gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat
    45541 aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg
    45601 ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta tttgtttcga
    45661 cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat
    45721 gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga
    45781 ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt
    45841 tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt
    45901 ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc
    45961 tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg
    46021 cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt
    46081 cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgctg
    46141 atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc
    46201 attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt
    46261 atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt
    46321 atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt
    46381 caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact
    46441 ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct
    46501 gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc
    46561 ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact
    46621 atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta
    46681 tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc
    46741 ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat
    46801 tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat
    46861 caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa
    46921 ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca
    46981 aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta
    47041 ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc
    47101 acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag
    47161 taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca
    47221 agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac
    47281 agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg
    47341 cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt
    47401 agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc
    47461 ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac
    47521 ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa
    47581 gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt
    47641 ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc
    47701 aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc
    47761 caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac
    47821 ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac
    47881 ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat
    47941 gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt
    48001 acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa
    48061 aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt
    48121 gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat
    48181 cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg
    48241 ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg
    48301 acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga
    48361 gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata
    48421 gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct
    48481 tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg
    48541 ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc
    48601 gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag
    48661 gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga
    48721 ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg
    48781 gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg
    48841 ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga
    48901 aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat
    48961 aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca
    49021 tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca
    49081 cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga
    49141 tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc
    49201 attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc
    49261 gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc
    49321 gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga
    49381 gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc
    49441 ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata
    49501 tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat
    49561 aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct
    49621 atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag
    49681 taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc
    49741 aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc
    49801 tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc
    49861 agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac
    49921 agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc
    49981 tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt
    50041 gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa
    50101 agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc
    50161 taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat
    50221 gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt
    50281 tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc
    50341 gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt
    50401 cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact
    50461 ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga
    50521 atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg
    50581 tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca
    50641 tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg
    50701 actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt
    50761 gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg
    50821 gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta
    50881 aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca
    50941 attaactgct gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg
    51001 atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca
    51061 actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt
    51121 ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca
    51181 agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca
    51241 tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca
    51301 aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc
    51361 ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc
    51421 gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc
    51481 caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt
    51541 acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct
    51601 tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga
    51661 atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt
    51721 tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct
    51781 aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt
    51841 agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt
    51901 ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct
    51961 aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc
    52021 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    52081 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    52141 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    52201 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    52261 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    52321 gaacgataat ggttatca
//
LOCUS       DACCXM010000008        46058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCXM010000008 DACCXM010000000
VERSION     DACCXM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.008"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            28..909
                     /locus_tag="IVY61_000380"
     CDS             28..909
                     /locus_tag="IVY61_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969521.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            922..2532
                     /locus_tag="IVY61_000381"
     CDS             922..2532
                     /locus_tag="IVY61_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP7969522.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2609..3268)
                     /locus_tag="IVY61_000382"
     CDS             complement(2609..3268)
                     /locus_tag="IVY61_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7969523.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3551..4216)
                     /locus_tag="IVY61_000383"
     CDS             complement(3551..4216)
                     /locus_tag="IVY61_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969524.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="IVY61_000384"
     CDS             complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="IVY61_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP7969525.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4996..5979)
                     /gene="dhaK"
                     /locus_tag="IVY61_000385"
     CDS             complement(4996..5979)
                     /gene="dhaK"
                     /locus_tag="IVY61_000385"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP7969526.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="IVY61_000386"
     CDS             complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="IVY61_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP7969527.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6530..6850
                     /locus_tag="IVY61_000387"
     CDS             6530..6850
                     /locus_tag="IVY61_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP7969528.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6962..7831)
                     /locus_tag="IVY61_000388"
     CDS             complement(6962..7831)
                     /locus_tag="IVY61_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7969529.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7927..9231)
                     /locus_tag="IVY61_000389"
     CDS             complement(7927..9231)
                     /locus_tag="IVY61_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7969530.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9228..9737)
                     /locus_tag="IVY61_000390"
     CDS             complement(9228..9737)
                     /locus_tag="IVY61_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969531.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9912..12047)
                     /locus_tag="IVY61_000391"
     CDS             complement(9912..12047)
                     /locus_tag="IVY61_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7969532.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12130..13785)
                     /locus_tag="IVY61_000392"
     CDS             complement(12130..13785)
                     /locus_tag="IVY61_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7969533.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13787..14101)
                     /locus_tag="IVY61_000393"
     CDS             complement(13787..14101)
                     /locus_tag="IVY61_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7969534.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14271..14531
                     /locus_tag="IVY61_000394"
     CDS             14271..14531
                     /locus_tag="IVY61_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP7969535.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14545..14859
                     /locus_tag="IVY61_000395"
     CDS             14545..14859
                     /locus_tag="IVY61_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP7969536.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15589..16143)
                     /locus_tag="IVY61_000396"
     CDS             complement(15589..16143)
                     /locus_tag="IVY61_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP7969537.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16269..18198)
                     /locus_tag="IVY61_000397"
                     /pseudo
     CDS             complement(16269..18198)
                     /locus_tag="IVY61_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18504..19220)
                     /locus_tag="IVY61_000398"
     CDS             complement(18504..19220)
                     /locus_tag="IVY61_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7969538.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19221..21044)
                     /gene="glpO"
                     /locus_tag="IVY61_000399"
     CDS             complement(19221..21044)
                     /gene="glpO"
                     /locus_tag="IVY61_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP7969539.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21058..22554)
                     /gene="glpK"
                     /locus_tag="IVY61_000400"
     CDS             complement(21058..22554)
                     /gene="glpK"
                     /locus_tag="IVY61_000400"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP7969540.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22690..24150)
                     /locus_tag="IVY61_000401"
     CDS             complement(22690..24150)
                     /locus_tag="IVY61_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7969541.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24140..25435)
                     /locus_tag="IVY61_000402"
     CDS             complement(24140..25435)
                     /locus_tag="IVY61_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7969542.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25767..26444)
                     /locus_tag="IVY61_000403"
     CDS             complement(25767..26444)
                     /locus_tag="IVY61_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP7969543.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26474..27565)
                     /locus_tag="IVY61_000404"
     CDS             complement(26474..27565)
                     /locus_tag="IVY61_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP7969544.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27666..28100)
                     /locus_tag="IVY61_000405"
     CDS             complement(27666..28100)
                     /locus_tag="IVY61_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7969545.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28684..30141)
                     /gene="ascB"
                     /locus_tag="IVY61_000406"
     CDS             complement(28684..30141)
                     /gene="ascB"
                     /locus_tag="IVY61_000406"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7969546.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30138..32033)
                     /locus_tag="IVY61_000407"
     CDS             complement(30138..32033)
                     /locus_tag="IVY61_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7969547.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32091..33866)
                     /locus_tag="IVY61_000408"
     CDS             complement(32091..33866)
                     /locus_tag="IVY61_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7969548.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33916..35412)
                     /locus_tag="IVY61_000409"
     CDS             complement(33916..35412)
                     /locus_tag="IVY61_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7969549.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35606..37228)
                     /locus_tag="IVY61_000410"
     CDS             complement(35606..37228)
                     /locus_tag="IVY61_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7969550.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37556..38869)
                     /locus_tag="IVY61_000411"
     CDS             complement(37556..38869)
                     /locus_tag="IVY61_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7969551.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39114..39338)
                     /locus_tag="IVY61_000412"
     CDS             complement(39114..39338)
                     /locus_tag="IVY61_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969552.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39358..39726)
                     /locus_tag="IVY61_000413"
     CDS             complement(39358..39726)
                     /locus_tag="IVY61_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7969553.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(39922..40632)
                     /locus_tag="IVY61_000414"
     CDS             complement(39922..40632)
                     /locus_tag="IVY61_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969554.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40650..41147)
                     /locus_tag="IVY61_000415"
     CDS             complement(40650..41147)
                     /locus_tag="IVY61_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7969555.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41184..41636)
                     /locus_tag="IVY61_000416"
     CDS             complement(41184..41636)
                     /locus_tag="IVY61_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7969556.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41771..42376)
                     /locus_tag="IVY61_000417"
     CDS             complement(41771..42376)
                     /locus_tag="IVY61_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7969557.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42826..43446)
                     /locus_tag="IVY61_000418"
     CDS             complement(42826..43446)
                     /locus_tag="IVY61_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7969558.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43448..44317)
                     /locus_tag="IVY61_000419"
     CDS             complement(43448..44317)
                     /locus_tag="IVY61_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP7969559.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44340..44747)
                     /locus_tag="IVY61_000420"
     CDS             complement(44340..44747)
                     /locus_tag="IVY61_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7969560.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44766..46055)
                     /locus_tag="IVY61_000421"
     CDS             complement(44766..46055)
                     /locus_tag="IVY61_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7969561.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        12923 a         9836 c         7889 g        15410 t
ORIGIN      
        1 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
       61 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
      121 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
      181 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
      241 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
      301 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
      361 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
      421 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
      481 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
      541 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
      601 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa
      661 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
      721 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
      781 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
      841 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
      901 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
      961 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
     1021 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
     1081 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
     1141 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
     1201 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
     1261 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
     1321 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
     1381 tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
     1441 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
     1501 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
     1561 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
     1621 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
     1681 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
     1741 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
     1801 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
     1861 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
     1921 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc
     1981 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
     2041 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
     2101 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
     2161 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
     2221 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
     2281 ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt
     2341 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
     2401 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
     2461 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
     2521 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
     2581 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
     2641 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
     2701 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
     2761 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaga gcgactcgtt
     2821 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
     2881 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
     2941 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
     3001 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
     3061 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
     3121 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
     3181 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
     3241 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
     3301 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
     3361 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
     3421 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
     3481 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
     3541 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
     3601 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
     3661 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
     3721 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
     3781 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
     3841 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
     3901 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
     3961 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
     4021 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
     4081 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
     4141 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
     4201 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
     4261 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
     4321 aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac
     4381 atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat
     4441 tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc
     4501 ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg
     4561 cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc
     4621 cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc
     4681 ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc
     4741 gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg
     4801 atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc
     4861 tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc
     4921 tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt
     4981 ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca
     5041 ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat
     5101 aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac
     5161 atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac
     5221 acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc
     5281 cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac
     5341 accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt
     5401 tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc
     5461 tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag
     5521 gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc
     5581 atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat
     5641 atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact
     5701 atcggcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc
     5761 aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc
     5821 gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg
     5881 ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc
     5941 ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc
     6001 cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg
     6061 ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct
     6121 cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa
     6181 aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt
     6241 ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt
     6301 gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca
     6361 aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg
     6421 ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta
     6481 gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac
     6541 aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga
     6601 ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg
     6661 cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga
     6721 aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta
     6781 tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc
     6841 cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc
     6901 actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt
     6961 tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta
     7021 aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt
     7081 tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt
     7141 ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt
     7201 ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac
     7261 tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct
     7321 gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga
     7381 cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc
     7441 ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt
     7501 tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca
     7561 aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa
     7621 tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt
     7681 tttcagcacc agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt
     7741 ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg
     7801 gcttcattct ttttttcatc acttttccca tccttactac tttttaacta atttcttatt
     7861 ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg
     7921 catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat
     7981 cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa
     8041 aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat
     8101 cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact
     8161 gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa
     8221 tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg
     8281 atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa
     8341 attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat
     8401 cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca
     8461 gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa
     8521 tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata
     8581 aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc
     8641 aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac
     8701 cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc
     8761 tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag
     8821 tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt
     8881 agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc
     8941 tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata
     9001 ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact
     9061 caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac
     9121 accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat
     9181 agacaaacaa gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc
     9241 accccatttt ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag
     9301 acagaataaa aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact
     9361 tcttcaagta attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt
     9421 acttttttgt atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa
     9481 ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa
     9541 aaagcgtaca aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat
     9601 ataaaaggaa gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt
     9661 aaccagtaat taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct
     9721 cttgttactt ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca
     9781 tacatttcat ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa
     9841 catttctcat cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt
     9901 ttatctctta attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc
     9961 gccgtgtaca tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag
    10021 attacatgat caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct
    10081 aatccatatg aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg
    10141 gtgataactg ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac
    10201 aattcttcag aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat
    10261 acgatgtcga tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg
    10321 ctagaacggt aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa
    10381 caacgcataa tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct
    10441 actaaagtaa agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta
    10501 taatcttgta cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca
    10561 agtaaatgac gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa
    10621 gcataagctg agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc
    10681 catgccattt cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt
    10741 gcataagcta caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac
    10801 tgcctatctt ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc
    10861 aaaagatgat tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc
    10921 aatttaggat gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact
    10981 gtttttccat ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct
    11041 gattgtttta gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca
    11101 ttttctcccg tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc
    11161 tgtgaaacca gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg
    11221 acattcgaaa tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat
    11281 gtcatcgtat ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt
    11341 ttaccacttc ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt
    11401 gtgttttgtt ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg
    11461 cctaagactt ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc
    11521 aaacgatctc tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt
    11581 tgacgaaccg tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt
    11641 gaacgccaat cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata
    11701 taaaaggatt cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga
    11761 cttttcaata aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc
    11821 atctgatcga tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag
    11881 taactgtcaa aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct
    11941 atttttgccg tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac
    12001 acgttcttca gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc
    12061 tttttttaaa gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt
    12121 taacgagtat catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga
    12181 aaatgcacca tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag
    12241 atagctacgt aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt
    12301 taatcggtcc cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc
    12361 caattcttct gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa
    12421 ttcatgccat tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag
    12481 catgtttact ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg
    12541 taaatcgaag atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc
    12601 cacacctttt gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat
    12661 caacttcagt aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac
    12721 atgaatgact tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga
    12781 tgcggcagtc attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg
    12841 agaaattccg gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag
    12901 agaaataagt gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc
    12961 ataaatatca ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc
    13021 taattcgatt ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat
    13081 aactaaatca gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc
    13141 aaatccagcg acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc
    13201 tataaaagct gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac
    13261 catgacttct aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc
    13321 accaacgatc acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc
    13381 tggtacattt ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac
    13441 aactggtttt gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc
    13501 ctcatgtacg acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg
    13561 acgcacatct aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc
    13621 ccgttcactg atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata
    13681 cggtcctttt tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc
    13741 agcagacatt ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta
    13801 ctgttcgatc aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt
    13861 tctttaattc tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg
    13921 tcttttcttt gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt
    13981 ttatatgccc gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca
    14041 aaagcttttc ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca
    14101 ttttctgcac ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt
    14161 tattaatatg tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa
    14221 aataccgata aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg
    14281 ataataaaaa tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa
    14341 aaatgatcga agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt
    14401 ctagtattga tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga
    14461 aacctgatag taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta
    14521 ttaaaaagta aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa
    14581 tggtcagaaa cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt
    14641 gtcgtatctg taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc
    14701 ttagatttgc aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt
    14761 tcagaaagtt ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt
    14821 gaactgcaat cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa
    14881 gtgatcagct tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg
    14941 aatcaaaggt tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata
    15001 gcttctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    15061 tttaaggaag aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa
    15121 aatagctcct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
    15181 cgtttaagga agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
    15241 aaaatggatt tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
    15301 taccgtttaa ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac
    15361 ggaaaaatag ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg
    15421 aacaccgttt accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt
    15481 tcaaaaacaa cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg
    15541 gagtctattt atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg
    15601 aaacatcttt cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat
    15661 gacggcaaaa atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat
    15721 catgccaaca tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt
    15781 gatattttta gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa
    15841 tagaccaaac aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag
    15901 ttgtgtcaat ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata
    15961 tctccgagca atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc
    16021 tgctatataa aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg
    16081 gaatatcgat aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt
    16141 cattttatcg ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat
    16201 aaaaccaatg atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt
    16261 tgagataatc acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt
    16321 tcttttacta gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta
    16381 cgtgttttat aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct
    16441 gctgaacgca ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat
    16501 acatcaatcg tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg
    16561 acaaatatcc gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt
    16621 gtaactgctg tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag
    16681 taacgctctg cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa
    16741 gcttggcttt ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg
    16801 atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc
    16861 tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc
    16921 gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac
    16981 caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa
    17041 tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa
    17101 gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt
    17161 caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat
    17221 caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa
    17281 tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc
    17341 attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc
    17401 gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac
    17461 ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga
    17521 aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc
    17581 atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac
    17641 aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt
    17701 tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga
    17761 cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt ctacatttat
    17821 caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc
    17881 aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt
    17941 tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc
    18001 cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg
    18061 acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct
    18121 atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact
    18181 aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat
    18241 aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt
    18301 attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa
    18361 ctttgtttat tggctattcg acttactgat tagataccag ctacactcac aaaacatacc
    18421 ccattcttct aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc
    18481 aaactttagt atgttttata gatttacacc attaatgaat agacacctac cgcgataatt
    18541 gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta
    18601 ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt
    18661 gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg
    18721 aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat
    18781 acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct
    18841 gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa
    18901 gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga
    18961 acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc
    19021 aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa
    19081 ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc
    19141 gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt
    19201 tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca
    19261 atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat
    19321 ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac
    19381 agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa
    19441 ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt
    19501 gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct
    19561 tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat
    19621 ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc
    19681 tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc
    19741 ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct
    19801 aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg
    19861 ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta
    19921 tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca
    19981 gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc
    20041 aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct
    20101 tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag
    20161 taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg
    20221 aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta
    20281 gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc
    20341 caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc
    20401 aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc
    20461 attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa
    20521 cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc
    20581 aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt
    20641 cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg
    20701 aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg
    20761 tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca
    20821 tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc
    20881 ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg
    20941 actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc
    21001 tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta
    21061 ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg
    21121 ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc
    21181 tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt
    21241 ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag
    21301 atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc
    21361 atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt
    21421 aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg
    21481 atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct
    21541 tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc
    21601 tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac
    21661 ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt
    21721 ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg
    21781 ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg
    21841 tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt
    21901 gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc
    21961 tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc
    22021 ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc
    22081 tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc
    22141 tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc
    22201 attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata
    22261 gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc
    22321 aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga
    22381 ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata
    22441 ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc
    22501 tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat
    22561 tccctccatt atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa
    22621 aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct
    22681 attttttaat caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat
    22741 gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca
    22801 aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc
    22861 gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact
    22921 ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac
    22981 attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa
    23041 ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat
    23101 tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa
    23161 aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt
    23221 caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc
    23281 ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat
    23341 cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc
    23401 tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg
    23461 atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt
    23521 tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc
    23581 taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag
    23641 cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag
    23701 ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt
    23761 caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg
    23821 aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc
    23881 ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc
    23941 ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat
    24001 atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt
    24061 gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc
    24121 ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca
    24181 tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg
    24241 tccatccttc ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc
    24301 tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt
    24361 tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat
    24421 aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat
    24481 tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga
    24541 catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg
    24601 gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca
    24661 agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct
    24721 gattgtcggg acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt
    24781 ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa
    24841 gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat
    24901 aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca
    24961 acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat
    25021 tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg
    25081 ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat
    25141 caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg
    25201 tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag
    25261 aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc
    25321 atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa
    25381 gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca
    25441 ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat
    25501 aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac
    25561 cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc
    25621 tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc
    25681 tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat
    25741 agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct
    25801 aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact
    25861 tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct
    25921 aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc
    25981 actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca
    26041 acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc
    26101 ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc
    26161 gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt
    26221 ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc
    26281 ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt
    26341 actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc
    26401 ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc
    26461 tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat
    26521 aaagctcttt atatgctttt tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca
    26581 ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg
    26641 catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag
    26701 ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat
    26761 cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct agttttgact
    26821 cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt
    26881 cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg
    26941 agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc
    27001 attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg
    27061 ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa
    27121 atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag
    27181 gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac
    27241 ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg
    27301 ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg
    27361 gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt
    27421 tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga
    27481 gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag
    27541 taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta
    27601 tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa
    27661 aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta
    27721 cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg
    27781 acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg
    27841 ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat
    27901 ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat
    27961 atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct
    28021 aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct
    28081 ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt
    28141 tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag
    28201 aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt
    28261 gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat
    28321 ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg
    28381 tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa
    28441 aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac
    28501 cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa
    28561 aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat
    28621 actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt
    28681 ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt
    28741 ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata
    28801 acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata
    28861 accaaataaa tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa
    28921 atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc
    28981 gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc
    29041 aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt
    29101 tttaattcct ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact
    29161 gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat
    29221 ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt
    29281 atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg
    29341 ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct
    29401 gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc
    29461 acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt
    29521 ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa
    29581 agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac
    29641 gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg
    29701 catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa
    29761 cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa
    29821 aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg
    29881 gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt
    29941 gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt
    30001 ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt
    30061 ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa
    30121 tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta
    30181 tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt
    30241 gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg
    30301 cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc
    30361 actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt
    30421 gcatgtttcg ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg
    30481 tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt
    30541 acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag
    30601 gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc
    30661 atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa
    30721 gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca
    30781 ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca
    30841 ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa
    30901 ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca
    30961 gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg
    31021 gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg
    31081 ccgataacag caccagataa aacagggcta aagttataaa cagctaaata tccagctgca
    31141 atcagctgac ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc
    31201 acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga
    31261 atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt
    31321 gtataccttg ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat
    31381 gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct
    31441 gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca
    31501 tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc
    31561 gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg
    31621 gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct
    31681 ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact
    31741 tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc
    31801 acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag
    31861 cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg
    31921 acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc
    31981 gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta
    32041 gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc
    32101 aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca
    32161 aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct
    32221 gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac
    32281 ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta
    32341 tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt
    32401 tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca
    32461 tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta
    32521 gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt
    32581 tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta
    32641 ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga
    32701 ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat
    32761 tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg
    32821 atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt
    32881 ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa
    32941 aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca
    33001 tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga
    33061 ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga
    33121 ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa
    33181 acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata
    33241 agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt
    33301 tgttgaggaa accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt
    33361 ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct
    33421 aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt
    33481 tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat
    33541 aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc
    33601 acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa
    33661 cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt tttgatttca
    33721 gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa
    33781 tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc
    33841 ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt
    33901 gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg
    33961 gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc
    34021 atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc
    34081 cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat
    34141 tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat
    34201 tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg
    34261 taattcataa cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg
    34321 ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc
    34381 gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa
    34441 tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg
    34501 tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat
    34561 gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg
    34621 atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc
    34681 atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg
    34741 acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc
    34801 agggaatgta accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt
    34861 aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa
    34921 ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa
    34981 gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt
    35041 gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg
    35101 aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa
    35161 acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag
    35221 tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg
    35281 ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca
    35341 tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat
    35401 ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg
    35461 attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct
    35521 actcttcatt tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca
    35581 ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc
    35641 gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta
    35701 aagcttcacg tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc
    35761 attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta
    35821 attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt
    35881 acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat
    35941 ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc
    36001 attgcatagg cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct
    36061 cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg
    36121 cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat
    36181 aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat
    36241 acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata
    36301 aactattcca tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt
    36361 cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta
    36421 tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa taaagtttag
    36481 cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag
    36541 ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt gtgatttctt
    36601 tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat
    36661 cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca
    36721 aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact
    36781 gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat
    36841 cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc
    36901 acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt
    36961 tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg
    37021 cttgataatt actgatatca tacccattgt catcattcgg agactcgtat actggagaaa
    37081 gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca
    37141 aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa
    37201 ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt
    37261 tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta
    37321 ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac
    37381 tgttttttga aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa
    37441 acggtgttca ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact
    37501 attttttcgt ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt
    37561 tcacattcaa tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag
    37621 atacttcttt tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg
    37681 caatcccatc tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga
    37741 tcgcaatctc taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta
    37801 ccataatcaa tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga
    37861 acaagcctaa caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa
    37921 cggcgatacc agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac
    37981 ccgcaagcat agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt
    38041 ctttcatgaa acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag
    38101 tcacaagcaa gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat
    38161 gttttaatgc aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag
    38221 gcattggaat caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga
    38281 tgccacctgg tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca
    38341 accaagtaga accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga
    38401 tgatcccacc ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg
    38461 catatttcaa atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga
    38521 tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc
    38581 ccattcctaa acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc
    38641 caattggata acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag
    38701 tcgcagtaaa cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa
    38761 ataagatata agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg
    38821 tggtgttcaa tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc
    38881 ctaaatccga acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt
    38941 gttttatcat atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat
    39001 ccgaacgttc catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat
    39061 tacaagaatt tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag
    39121 tgtgatttga ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca
    39181 ggattcagat tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc
    39241 ttcttttttc ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga
    39301 tacttcaaat aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca
    39361 atcagacgac tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa
    39421 agtcaagatg ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt
    39481 ttcaaattca tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa
    39541 cttctgcaca aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac
    39601 aatactctta aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc
    39661 tacaaagaat cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg
    39721 tatcatcatt tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat
    39781 tatatcacga aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag
    39841 tttctaggat tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc
    39901 gctcttctta tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg
    39961 tctaacacta gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg
    40021 tgcgccacgt gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt
    40081 ttcttccgtc agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc
    40141 ttgttcatca tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa
    40201 aggcaggaat gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc
    40261 gatatcttct ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac
    40321 aagcggattg attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat
    40381 agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat
    40441 gacttcgttt tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc
    40501 attcaataat tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt
    40561 tacggataag atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat
    40621 tacttttttc atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct
    40681 gtttggggat catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta
    40741 cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt
    40801 tgcttttctt ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt
    40861 ttaaattttt ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca
    40921 gattgactcg ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact
    40981 aggaaaaaat cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct
    41041 tgtgtcaagt aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat
    41101 gtttcgatac taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg
    41161 tatttattca cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc
    41221 caatcttcgc taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct
    41281 tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa
    41341 ggatagattt ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta
    41401 tcttccaaac gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt
    41461 gtacgatcta ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa
    41521 atacgtacta aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg
    41581 ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca
    41641 tcccaccttt attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt
    41701 ttcaataaaa aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt
    41761 acgaaaaagc tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct
    41821 tcgttgttcg cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg
    41881 aagtttcatt attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa
    41941 gtttataata ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc
    42001 gcaactgagt agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt
    42061 cttcaggaga caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt
    42121 tatcttcgat tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg
    42181 gtgttggatg tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc
    42241 ctatttcact gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa
    42301 atacatcttt tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag
    42361 ccaacttctt gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag
    42421 tatagaagac tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga
    42481 atgttaaaaa actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg
    42541 ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc
    42601 ttgtctggct gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac
    42661 ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg
    42721 tttttttgaa attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta
    42781 aacagcgttc aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt
    42841 gatttctttt tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac
    42901 atcaaattca tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt
    42961 tttgaatgct tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc
    43021 atagtgataa tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta
    43081 ccgacgttgc cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc
    43141 gtatctatct gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga
    43201 cacaatcact ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata
    43261 aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc
    43321 atagtattcc ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag
    43381 tttattcata agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt
    43441 tttcatctca tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct
    43501 tatgcttgac aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact
    43561 tgttgatttc tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct
    43621 ccgttccaac attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt
    43681 tcacacctgt tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca
    43741 gcaagggaat gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa
    43801 tagctaatgc atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat
    43861 ataccttgtc attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt
    43921 ctccttctac ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga
    43981 tattttcttt gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga
    44041 ttgcttcttt gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca
    44101 tttcttcttc tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag
    44161 cggtatcgac acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca
    44221 tagcaggaag catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt
    44281 ctgctgtttt tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt
    44341 tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc
    44401 atgtagtaat cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta
    44461 tagaggctca tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt
    44521 tctaaatatt ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt
    44581 tcataaagtt tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt
    44641 gtaatctctg attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc
    44701 agaacttttt tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact
    44761 ttcaattaaa atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct
    44821 actgtatcta catcggttgc tgtagcattg ttgaatccca tcgaattaaa gatcgttact
    44881 agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct
    44941 cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt
    45001 agcataccag gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc
    45061 gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa
    45121 actggacaat ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat
    45181 gccggcacga tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact
    45241 acgaattgga tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca
    45301 gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc
    45361 atgacagaca aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg
    45421 atcgactttt tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag
    45481 tacccgatcg tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg
    45541 atcggttgcg ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc
    45601 aagatcaata cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc
    45661 atccataaaa ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa
    45721 ataatattga atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa
    45781 tcattcattg cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat
    45841 ccttcagtaa agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca
    45901 ctcaacactt gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat
    45961 ttcttctgca gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc
    46021 aaaatatttt cgatgataaa attgataacg cccatgtt
//
LOCUS       DACCXM010000009        44832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCXM010000009 DACCXM010000000
VERSION     DACCXM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.009"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(2..1012)
                     /locus_tag="IVY61_000422"
     CDS             complement(2..1012)
                     /locus_tag="IVY61_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7969562.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1192..1536)
                     /locus_tag="IVY61_000423"
     CDS             complement(1192..1536)
                     /locus_tag="IVY61_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7969563.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1529..1963)
                     /locus_tag="IVY61_000424"
     CDS             complement(1529..1963)
                     /locus_tag="IVY61_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP7969564.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2101..2838
                     /locus_tag="IVY61_000425"
     CDS             2101..2838
                     /locus_tag="IVY61_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969565.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2831..4042
                     /locus_tag="IVY61_000426"
     CDS             2831..4042
                     /locus_tag="IVY61_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7969566.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4249..5028
                     /locus_tag="IVY61_000427"
     CDS             4249..5028
                     /locus_tag="IVY61_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP7969567.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5098..5760
                     /gene="trmB"
                     /locus_tag="IVY61_000428"
     CDS             5098..5760
                     /gene="trmB"
                     /locus_tag="IVY61_000428"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP7969568.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5858..7612)
                     /gene="ade"
                     /locus_tag="IVY61_000429"
     CDS             complement(5858..7612)
                     /gene="ade"
                     /locus_tag="IVY61_000429"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP7969569.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7744..8673
                     /gene="cpaF"
                     /locus_tag="IVY61_000430"
     CDS             7744..8673
                     /gene="cpaF"
                     /locus_tag="IVY61_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP7969570.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8630..9679
                     /locus_tag="IVY61_000431"
     CDS             8630..9679
                     /locus_tag="IVY61_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP7969571.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9676..9954
                     /locus_tag="IVY61_000432"
     CDS             9676..9954
                     /locus_tag="IVY61_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP7969572.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9929..10399
                     /locus_tag="IVY61_000433"
     CDS             9929..10399
                     /locus_tag="IVY61_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP7969573.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10416..10718
                     /locus_tag="IVY61_000434"
     CDS             10416..10718
                     /locus_tag="IVY61_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969574.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10735..11106
                     /locus_tag="IVY61_000435"
     CDS             10735..11106
                     /locus_tag="IVY61_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP7969575.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            11078..11437
                     /locus_tag="IVY61_000436"
     CDS             11078..11437
                     /locus_tag="IVY61_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969576.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11609..12616
                     /locus_tag="IVY61_000437"
     CDS             11609..12616
                     /locus_tag="IVY61_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7969577.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12641..13825
                     /locus_tag="IVY61_000438"
     CDS             12641..13825
                     /locus_tag="IVY61_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP7969578.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14262..15845)
                     /locus_tag="IVY61_000439"
     CDS             complement(14262..15845)
                     /locus_tag="IVY61_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7969579.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16423..16566)
                     /locus_tag="IVY61_000440"
     CDS             complement(16423..16566)
                     /locus_tag="IVY61_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP7969580.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16789..17268)
                     /locus_tag="IVY61_000441"
     CDS             complement(16789..17268)
                     /locus_tag="IVY61_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7969581.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17341..17625)
                     /locus_tag="IVY61_000442"
     CDS             complement(17341..17625)
                     /locus_tag="IVY61_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969582.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17703..17897)
                     /gene="ssrS"
                     /locus_tag="IVY61_000443"
     ncRNA           complement(17703..17897)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVY61_000443"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17969..19255)
                     /locus_tag="IVY61_000444"
     CDS             complement(17969..19255)
                     /locus_tag="IVY61_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP7969583.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19393..20064
                     /locus_tag="IVY61_000445"
     CDS             19393..20064
                     /locus_tag="IVY61_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP7969584.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20030..20518
                     /locus_tag="IVY61_000446"
     CDS             20030..20518
                     /locus_tag="IVY61_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP7969585.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20531..21478
                     /gene="prmA"
                     /locus_tag="IVY61_000447"
     CDS             20531..21478
                     /gene="prmA"
                     /locus_tag="IVY61_000447"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP7969586.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21479..22243
                     /locus_tag="IVY61_000448"
     CDS             21479..22243
                     /locus_tag="IVY61_000448"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP7969587.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22476..24689
                     /locus_tag="IVY61_000449"
     CDS             22476..24689
                     /locus_tag="IVY61_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP7969588.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24700..25146
                     /locus_tag="IVY61_000450"
     CDS             24700..25146
                     /locus_tag="IVY61_000450"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP7969589.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25317..26576
                     /locus_tag="IVY61_000451"
     CDS             25317..26576
                     /locus_tag="IVY61_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP7969590.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26665..27612)
                     /locus_tag="IVY61_000452"
     CDS             complement(26665..27612)
                     /locus_tag="IVY61_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7969591.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28006..29298
                     /locus_tag="IVY61_000453"
     CDS             28006..29298
                     /locus_tag="IVY61_000453"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP7969592.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29313..31082
                     /gene="aspS"
                     /locus_tag="IVY61_000454"
     CDS             29313..31082
                     /gene="aspS"
                     /locus_tag="IVY61_000454"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP7969593.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31191..32066
                     /locus_tag="IVY61_000455"
     CDS             31191..32066
                     /locus_tag="IVY61_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7969594.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32332..34215
                     /locus_tag="IVY61_000456"
     CDS             32332..34215
                     /locus_tag="IVY61_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7969595.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34290..34574)
                     /locus_tag="IVY61_000457"
     CDS             complement(34290..34574)
                     /locus_tag="IVY61_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7969596.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34564..35298)
                     /locus_tag="IVY61_000458"
     CDS             complement(34564..35298)
                     /locus_tag="IVY61_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP7969597.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35427..36050
                     /locus_tag="IVY61_000459"
     CDS             35427..36050
                     /locus_tag="IVY61_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP7969598.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36242..37363
                     /locus_tag="IVY61_000460"
     CDS             36242..37363
                     /locus_tag="IVY61_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP7969599.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37370..40498
                     /locus_tag="IVY61_000461"
     CDS             37370..40498
                     /locus_tag="IVY61_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP7969600.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40517..43711
                     /locus_tag="IVY61_000462"
     CDS             40517..43711
                     /locus_tag="IVY61_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7969601.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43857..44564)
                     /locus_tag="IVY61_000463"
     CDS             complement(43857..44564)
                     /locus_tag="IVY61_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7969602.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14383 a         7961 c         9406 g        13082 t
ORIGIN      
        1 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
       61 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
      121 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
      181 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
      241 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
      301 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
      361 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
      421 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
      481 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
      541 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
      601 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
      661 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
      721 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
      781 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
      841 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
      901 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
      961 ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc attaaaattt
     1021 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
     1081 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
     1141 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
     1201 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
     1261 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
     1321 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
     1381 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
     1441 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
     1501 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
     1561 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
     1621 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
     1681 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
     1741 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
     1801 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
     1861 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
     1921 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
     1981 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     2041 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     2101 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     2161 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     2221 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     2281 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     2341 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     2401 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     2461 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     2521 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     2581 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     2641 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     2701 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     2761 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     2821 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     2881 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     2941 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     3001 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     3061 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     3121 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     3181 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     3241 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     3301 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     3361 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     3421 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     3481 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     3541 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     3601 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     3661 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     3721 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
     3781 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
     3841 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
     3901 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
     3961 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
     4021 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
     4081 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
     4141 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
     4201 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
     4261 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
     4321 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
     4381 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
     4441 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
     4501 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
     4561 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
     4621 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
     4681 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
     4741 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
     4801 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
     4861 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
     4921 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
     4981 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
     5041 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
     5101 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
     5161 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
     5221 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
     5281 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
     5341 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
     5401 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
     5461 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
     5521 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
     5581 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
     5641 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
     5701 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
     5761 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatccca gaaaatgaac
     5821 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
     5881 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
     5941 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
     6001 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
     6061 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
     6121 gctgattgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
     6181 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
     6241 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
     6301 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
     6361 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
     6421 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
     6481 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
     6541 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
     6601 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
     6661 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
     6721 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
     6781 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
     6841 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
     6901 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
     6961 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
     7021 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
     7081 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
     7141 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
     7201 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
     7261 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
     7321 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
     7381 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
     7441 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
     7501 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
     7561 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
     7621 ctcccaagat aaatttttat aagtataaca taaattcggt ttttgccaat atttttttgt
     7681 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
     7741 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
     7801 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
     7861 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
     7921 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
     7981 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
     8041 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
     8101 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
     8161 agcggaccag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
     8221 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
     8281 cagatcaatc cgaaaatagc acagacttat gatacacttt tgagactcgc attacggcat
     8341 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
     8401 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
     8461 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
     8521 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
     8581 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
     8641 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
     8701 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
     8761 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
     8821 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
     8881 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
     8941 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
     9001 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
     9061 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
     9121 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
     9181 gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
     9241 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
     9301 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
     9361 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
     9421 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
     9481 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
     9541 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
     9601 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
     9661 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
     9721 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
     9781 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
     9841 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
     9901 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
     9961 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
    10021 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
    10081 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
    10141 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
    10201 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
    10261 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
    10321 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
    10381 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
    10441 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
    10501 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
    10561 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
    10621 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
    10681 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
    10741 ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
    10801 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
    10861 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
    10921 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
    10981 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    11041 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    11101 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    11161 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    11221 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    11281 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    11341 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    11401 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    11461 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    11521 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    11581 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    11641 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    11701 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    11761 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    11821 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    11881 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    11941 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    12001 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    12061 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    12121 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    12181 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    12241 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    12301 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    12361 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    12421 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    12481 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    12541 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    12601 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    12661 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    12721 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    12781 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    12841 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    12901 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    12961 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    13021 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    13081 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    13141 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    13201 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    13261 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    13321 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    13381 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    13441 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    13501 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    13561 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    13621 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    13681 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    13741 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    13801 tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag atgaaacgca
    13861 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    13921 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    13981 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    14041 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    14101 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    14161 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    14221 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    14281 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    14341 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    14401 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    14461 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    14521 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    14581 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    14641 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    14701 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    14761 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    14821 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    14881 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    14941 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    15001 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    15061 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    15121 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    15181 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    15241 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    15301 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    15361 cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag
    15421 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    15481 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    15541 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    15601 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    15661 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    15721 gatttgccag ttccgttgat accgactaag acaatttttt caccagcttc aatttgacag
    15781 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    15841 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccatg gcctgtgtta
    15901 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    15961 caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    16021 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    16081 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    16141 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt
    16201 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    16261 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    16321 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    16381 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    16441 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    16501 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    16561 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    16621 cgactaatcg ccctggtgtt cacttttttt tattttactt gaactcattg actgttttcc
    16681 ctttatttta aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg
    16741 gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt
    16801 gtggtcttta gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc
    16861 tgcgtttcta ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa
    16921 tcctgtggct cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat
    16981 aatttgtttt ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt
    17041 cgcattgctt tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag
    17101 cattccatca aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag
    17161 aagttctccg ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc
    17221 atcagaacca tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc
    17281 ctcaagtcaa tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat
    17341 ttactgcttt ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat
    17401 aattacgatc agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat
    17461 acccattacg cctaaaacag catatgcagc tccgtaaaat tgtaggaatc cttgattttt
    17521 atcatttttg ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac
    17581 gaataataat actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa
    17641 aataagcaac aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga
    17701 tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag
    17761 gtgggtccca tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac
    17821 acaagacggg tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc
    17881 gaacatcaca gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga
    17941 gctcgcagaa acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt
    18001 ttatacggta atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat
    18061 aatactgagc attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa
    18121 aatgatgagg atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc
    18181 tatctctcaa atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg
    18241 catctaatgc catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt
    18301 ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca
    18361 ctgtacgagc cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca
    18421 gacgccggca aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg
    18481 cagcatccac atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag
    18541 catctccatt tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca
    18601 gtgtaagatg aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc
    18661 cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt
    18721 cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga
    18781 ctgccaactg gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc
    18841 ggatcgctgg attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc
    18901 ggccactttc taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt
    18961 catccaataa gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat
    19021 ccttttttga atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga
    19081 tcgcacttgc gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga
    19141 gcatgcgagc atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac
    19201 caacgacttc atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat
    19261 tttcctcctt cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa
    19321 gaacagcctt ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag
    19381 taggtgagaa aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg
    19441 ccagaagtgg cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga
    19501 ttagctggat atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat
    19561 agctatggtt taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc
    19621 tacctttaca cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt
    19681 cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa
    19741 aatcgtggcg gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca
    19801 ttagcaattc ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt
    19861 tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac
    19921 aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct
    19981 ttacaaagaa aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa
    20041 ggatctatta acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc
    20101 tttagattgg gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa
    20161 taaagcacaa tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga
    20221 atttgaagac ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta
    20281 tctagcagta atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt
    20341 agtggaatat ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga
    20401 tgaagacgat gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt
    20461 agtagaagta aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg
    20521 agtgacagat atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc
    20581 agttgctaat attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga
    20641 tgttgaacat tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc
    20701 gctgaaagaa ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat
    20761 cttgcctttt atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa
    20821 aaataaagta acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata
    20881 ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca
    20941 gacagaagat cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg
    21001 cactcatccc acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga
    21061 aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg
    21121 tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa
    21181 tatggatatg aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg
    21241 aatcgagatt gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat
    21301 gatcccagat gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat
    21361 tgaagagaag aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca
    21421 aattttccag caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat
    21481 gcagcggtat tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga
    21541 aaactatcac cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc
    21601 attcaatgat caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta
    21661 tctgaaagaa attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc
    21721 gagtggctat cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg
    21781 agcgcaagct tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa
    21841 acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca
    21901 tcggcagatc gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct
    21961 agatgattat gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc
    22021 aaatgcagcc aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg
    22081 accagaaggt ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact
    22141 ttgtggatta ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc
    22201 tgctagctat caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa
    22261 agagtatcga cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat
    22321 ttatacaatg gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa
    22381 aaaaacgtat aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc
    22441 cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg
    22501 gacccggtgt cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg
    22561 agaaagcatg cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac
    22621 cctacattat tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc
    22681 atacagtggc tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag
    22741 atctgagaga agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag
    22801 ggaaaataaa atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc
    22861 tagcaatggc tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca
    22921 tgcgtacgtt gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag
    22981 aaatctatgc gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag
    23041 atacagcact tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga
    23101 cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga
    23161 cagaagagct ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct
    23221 atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc
    23281 gagtgatcgt agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat
    23341 ggaaaccaat gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc
    23401 aatctttgca tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa
    23461 cacaggaaat gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag
    23521 gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga
    23581 tactcgaact tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag
    23641 atatctttag tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa
    23701 aaggatccgg accattggac tttgcataca gtatccatac agatatcggt aacaaaacca
    23761 ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata
    23821 tcattgagat catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag
    23881 ttgctactag caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag
    23941 aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc
    24001 ctaaagatat tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa
    24061 cagaagatga tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca
    24121 atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag
    24181 aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg
    24241 gcgttgtcat tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga
    24301 ttcctggtga tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc
    24361 gagattgtcc gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg
    24421 aatgggaaga tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct
    24481 ataatcgttc aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc
    24541 tcaacagcgt tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg
    24601 gtatccagaa tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg
    24661 tctacagtgt acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg
    24721 gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta
    24781 cttgttttat taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa
    24841 aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa
    24901 gatgtgggag gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa
    24961 ggaaatcgac caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa
    25021 tcattcaatg aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca
    25081 gatatgtcgg tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac
    25141 aaataaagta atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact
    25201 gataaactct aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc
    25261 ggaaaaaaag ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga
    25321 aagaagtacc agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat
    25381 acaacatgtt ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt
    25441 tcaataaaga agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa
    25501 ggaatttcaa acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag
    25561 gtggagtaca gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc
    25621 taatgacacc ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag
    25681 cacttgaagc acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta
    25741 catctatttg cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa
    25801 aattagcaga atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca
    25861 taaaaggtgt cgaaaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag
    25921 ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca
    25981 cagatttaga tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca
    26041 tccgtccaac ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta
    26101 gaccagattt gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc
    26161 aagaaatgag acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag
    26221 tagaagtaga tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa
    26281 cagaccatga tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag
    26341 ggaaagcaaa agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa
    26401 attttcgaca aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt
    26461 tttccgatca gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc
    26521 cattcaatca gtttccgact tatccgaata gccgagcgct actttacaag aattagatta
    26581 gcataaactt atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta
    26641 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
    26701 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
    26761 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
    26821 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
    26881 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
    26941 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
    27001 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
    27061 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
    27121 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
    27181 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
    27241 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
    27301 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
    27361 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
    27421 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
    27481 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
    27541 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
    27601 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
    27661 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
    27721 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
    27781 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
    27841 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
    27901 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
    27961 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
    28021 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
    28081 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
    28141 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
    28201 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
    28261 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
    28321 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
    28381 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
    28441 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
    28501 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
    28561 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
    28621 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
    28681 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
    28741 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
    28801 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
    28861 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
    28921 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
    28981 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
    29041 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
    29101 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
    29161 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
    29221 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
    29281 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
    29341 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
    29401 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
    29461 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
    29521 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
    29581 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
    29641 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
    29701 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
    29761 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
    29821 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
    29881 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
    29941 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
    30001 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
    30061 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
    30121 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
    30181 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
    30241 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
    30301 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
    30361 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
    30421 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
    30481 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
    30541 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
    30601 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
    30661 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
    30721 tctatgcaga agcttatgat atcgtcttga acggttatga actaggcgga ggttctatcc
    30781 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
    30841 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
    30901 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
    30961 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
    31021 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
    31081 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
    31141 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
    31201 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
    31261 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
    31321 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
    31381 taccggtatc aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa
    31441 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
    31501 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
    31561 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
    31621 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
    31681 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
    31741 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
    31801 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
    31861 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
    31921 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
    31981 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
    32041 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
    32101 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
    32161 ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt
    32221 tatgggatga tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt
    32281 aatacccgta tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa
    32341 caaaaaaggt taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct
    32401 gtggtgggtg ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc
    32461 gttccgattt ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg
    32521 gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat
    32581 ccggcacttg tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg
    32641 ctgttaaaca atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg
    32701 caaatcaata acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat
    32761 atctggtctt tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg
    32821 ttgatggtat ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc
    32881 ctctccgcca tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat
    32941 tacggtaccg acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg
    33001 ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct
    33061 ggaggaatct cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat
    33121 ttcatctatt acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg
    33181 acagcacacc ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt
    33241 ggcaaacgaa gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca
    33301 aaggtgactg atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt
    33361 ctaatcatca gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat
    33421 ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa
    33481 ccatgggttg ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca
    33541 ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa
    33601 aaattagcag aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg
    33661 atccttgata agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta
    33721 acggcatttg gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca
    33781 aaagtagtgg tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa
    33841 aaattaaagg aacaagattt gttgaaagac actgttctga tcggactagg aacgaatggc
    33901 tcattctcgg aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac
    33961 tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa
    34021 cagatgacga aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac
    34081 catagtgact ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac
    34141 attcacttag tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca
    34201 aacgtgacag aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag
    34261 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
    34321 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
    34381 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
    34441 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
    34501 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
    34561 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
    34621 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
    34681 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
    34741 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
    34801 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
    34861 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
    34921 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
    34981 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
    35041 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
    35101 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
    35161 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
    35221 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
    35281 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
    35341 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
    35401 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
    35461 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
    35521 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
    35581 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
    35641 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
    35701 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
    35761 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
    35821 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
    35881 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
    35941 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
    36001 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
    36061 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
    36121 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
    36181 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
    36241 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
    36301 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
    36361 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
    36421 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
    36481 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
    36541 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
    36601 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
    36661 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
    36721 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
    36781 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
    36841 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
    36901 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
    36961 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
    37021 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
    37081 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
    37141 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
    37201 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
    37261 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
    37321 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
    37381 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
    37441 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
    37501 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
    37561 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
    37621 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
    37681 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
    37741 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
    37801 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
    37861 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
    37921 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
    37981 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
    38041 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
    38101 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
    38161 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
    38221 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
    38281 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
    38341 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
    38401 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
    38461 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
    38521 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
    38581 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
    38641 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
    38701 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
    38761 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
    38821 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
    38881 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
    38941 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
    39001 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
    39061 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
    39121 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
    39181 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
    39241 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
    39301 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
    39361 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
    39421 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
    39481 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
    39541 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
    39601 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
    39661 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
    39721 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
    39781 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
    39841 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
    39901 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
    39961 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
    40021 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
    40081 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
    40141 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
    40201 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
    40261 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
    40321 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
    40381 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
    40441 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
    40501 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
    40561 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
    40621 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
    40681 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
    40741 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
    40801 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
    40861 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
    40921 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
    40981 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
    41041 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
    41101 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
    41161 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
    41221 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
    41281 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
    41341 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
    41401 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
    41461 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
    41521 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
    41581 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
    41641 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
    41701 tggcgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
    41761 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
    41821 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
    41881 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
    41941 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
    42001 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
    42061 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
    42121 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
    42181 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
    42241 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
    42301 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
    42361 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
    42421 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
    42481 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
    42541 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
    42601 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
    42661 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
    42721 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
    42781 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
    42841 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
    42901 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
    42961 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
    43021 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
    43081 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
    43141 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
    43201 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
    43261 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
    43321 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
    43381 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
    43441 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
    43501 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
    43561 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
    43621 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
    43681 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
    43741 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
    43801 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
    43861 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
    43921 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
    43981 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
    44041 agcatatgaa attctttctc agctagctga aaaatctctt tttctggttt ttggaaacct
    44101 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
    44161 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
    44221 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    44281 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    44341 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    44401 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    44461 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    44521 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    44581 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    44641 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    44701 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    44761 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa
    44821 agatttattt tt
//
LOCUS       DACCXM010000010        38435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCXM010000010 DACCXM010000000
VERSION     DACCXM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.010"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            572..1456
                     /locus_tag="IVY61_000464"
     CDS             572..1456
                     /locus_tag="IVY61_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7969603.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            1665..2243
                     /gene="pgsA"
                     /locus_tag="IVY61_000465"
     CDS             1665..2243
                     /gene="pgsA"
                     /locus_tag="IVY61_000465"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP7969604.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            2483..3724
                     /locus_tag="IVY61_000466"
     CDS             2483..3724
                     /locus_tag="IVY61_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP7969605.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            3833..4882
                     /gene="recA"
                     /locus_tag="IVY61_000467"
     CDS             3833..4882
                     /gene="recA"
                     /locus_tag="IVY61_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP7969606.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            5293..6849
                     /gene="rny"
                     /locus_tag="IVY61_000468"
     CDS             5293..6849
                     /gene="rny"
                     /locus_tag="IVY61_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP7969607.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6928..7734)
                     /gene="proC"
                     /locus_tag="IVY61_000469"
     CDS             complement(6928..7734)
                     /gene="proC"
                     /locus_tag="IVY61_000469"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP7969608.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            7875..10448
                     /gene="mprF"
                     /locus_tag="IVY61_000470"
     CDS             7875..10448
                     /gene="mprF"
                     /locus_tag="IVY61_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7969609.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            10602..11453
                     /locus_tag="IVY61_000471"
     CDS             10602..11453
                     /locus_tag="IVY61_000471"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP7969610.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            11698..12636
                     /locus_tag="IVY61_000472"
     CDS             11698..12636
                     /locus_tag="IVY61_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP7969611.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            12659..13339
                     /locus_tag="IVY61_000473"
     CDS             12659..13339
                     /locus_tag="IVY61_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7969612.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            13312..14136
                     /locus_tag="IVY61_000474"
     CDS             13312..14136
                     /locus_tag="IVY61_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7969613.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            14303..15121
                     /gene="purR"
                     /locus_tag="IVY61_000475"
     CDS             14303..15121
                     /gene="purR"
                     /locus_tag="IVY61_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP7969614.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            15624..16997
                     /gene="glmU"
                     /locus_tag="IVY61_000476"
     CDS             15624..16997
                     /gene="glmU"
                     /locus_tag="IVY61_000476"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP7969615.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            17091..18062
                     /locus_tag="IVY61_000477"
     CDS             17091..18062
                     /locus_tag="IVY61_000477"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7969616.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      18068..18170
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            18236..18805
                     /gene="thiT"
                     /locus_tag="IVY61_000478"
     CDS             18236..18805
                     /gene="thiT"
                     /locus_tag="IVY61_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP7969617.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(18886..19659)
                     /locus_tag="IVY61_000479"
     CDS             complement(18886..19659)
                     /locus_tag="IVY61_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969618.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            19926..21626
                     /locus_tag="IVY61_000480"
     CDS             19926..21626
                     /locus_tag="IVY61_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP7969619.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            21596..21814
                     /locus_tag="IVY61_000481"
     CDS             21596..21814
                     /locus_tag="IVY61_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969620.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            21834..22724
                     /locus_tag="IVY61_000482"
     CDS             21834..22724
                     /locus_tag="IVY61_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP7969621.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            22742..23026
                     /locus_tag="IVY61_000483"
     CDS             22742..23026
                     /locus_tag="IVY61_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969622.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            23513..24127
                     /gene="gmk"
                     /locus_tag="IVY61_000484"
     CDS             23513..24127
                     /gene="gmk"
                     /locus_tag="IVY61_000484"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP7969623.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            24132..24437
                     /locus_tag="IVY61_000485"
     CDS             24132..24437
                     /locus_tag="IVY61_000485"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7969624.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            24690..27098
                     /gene="priA"
                     /locus_tag="IVY61_000486"
     CDS             24690..27098
                     /gene="priA"
                     /locus_tag="IVY61_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP7969625.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            27113..27604
                     /gene="def"
                     /locus_tag="IVY61_000487"
     CDS             27113..27604
                     /gene="def"
                     /locus_tag="IVY61_000487"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7969626.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            27597..28535
                     /locus_tag="IVY61_000488"
     CDS             27597..28535
                     /locus_tag="IVY61_000488"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP7969627.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            28535..29893
                     /gene="rsmB"
                     /locus_tag="IVY61_000489"
     CDS             28535..29893
                     /gene="rsmB"
                     /locus_tag="IVY61_000489"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP7969628.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            29906..30646
                     /locus_tag="IVY61_000490"
     CDS             29906..30646
                     /locus_tag="IVY61_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP7969629.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            30643..32712
                     /gene="pknB"
                     /locus_tag="IVY61_000491"
     CDS             30643..32712
                     /gene="pknB"
                     /locus_tag="IVY61_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP7969630.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            32877..33776
                     /gene="rsgA"
                     /locus_tag="IVY61_000492"
     CDS             32877..33776
                     /gene="rsgA"
                     /locus_tag="IVY61_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP7969631.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            33789..34439
                     /locus_tag="IVY61_000493"
     CDS             33789..34439
                     /locus_tag="IVY61_000493"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7969632.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            34440..35078
                     /locus_tag="IVY61_000494"
     CDS             34440..35078
                     /locus_tag="IVY61_000494"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP7969633.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            35233..36087
                     /gene="mreC"
                     /locus_tag="IVY61_000495"
     CDS             35233..36087
                     /gene="mreC"
                     /locus_tag="IVY61_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP7969634.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            36091..36600
                     /gene="mreD"
                     /locus_tag="IVY61_000496"
     CDS             36091..36600
                     /gene="mreD"
                     /locus_tag="IVY61_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP7969635.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            36855..38429
                     /locus_tag="IVY61_000497"
     CDS             36855..38429
                     /locus_tag="IVY61_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969636.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT        12645 a         6874 c         8362 g        10554 t
ORIGIN      
        1 agaaataaga aggaatttac gaaaattgct tctcaaattt ttgtgatatt tcggcttatt
       61 gccgaagaag ctacttctgg aacaccgtgt accaagaggt tgtgaaaaag ctgccctgca
      121 tcaagtaata agaacagcca agtaaaaaca gcttcttaat gtttttaact tggttggact
      181 tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga aatcagcgtt
      241 ttgacctgag tattagagaa acatacggaa aaatatctcc ttatattttg ccggtttttg
      301 agctaatacc gaggaacagc tgattgaaca ccgtttaaga aagaggtcgt gagcctgccg
      361 tttaactccg agtcacaagg aacaagttca aaaaacagct tcttatattt attggatatt
      421 gtgacttgtg accgaggagt tggcaggaga acaatgttta ttcggaaaaa aggagctgtg
      481 acatgacttt tgtcacagca cccaaacata acgtctttcg ttgcaacagc agatgcttcg
      541 gagaatcaaa cagactggag aagatgaacg agtggctttt gaaaccatcg gagaaaaatt
      601 acggcaagca cgcctgaata aaaaaatatc tttagatgaa ttacagcaga ttacaaaaat
      661 tcaaaaacgt tatttggaag caatcgacaa tgatgatttt gaccaattgc ctggtaaatt
      721 ttacgtgcgg gcatttattc ggcaatacgc tgaagctgta ggagaagatg gagaccattt
      781 agtcgatgtt tttgatggga aaagcaaatt gacaggatcg gtccctgaac gtccgcagcc
      841 tgaaacagta agtacttcta gaaaagcaat gcatcaggaa gaatcgcatc caagtcgctt
      901 ttggacaagt cttcctgtca ttttattagg attggttgct ttggcgattg tcgttgtcgt
      961 gggctatatg acttggcaag atcgtcaagc tgatccaata atcggagaca cagcttcttc
     1021 gcttaaagta gaaggatcag tcgaggagtc ctcttcctca gagcaaagca cgcagaccaa
     1081 agagtcttct acagtagaat caacaacaaa atcaagtgaa gaaaagaaaa tggcaatagc
     1141 tacaggagaa gattctggca gtgccatcgc agtaaatatc acagacgcta ccaagcctgt
     1201 aacccttgaa tttactgctg tcaatcgtgt atggttaggt gtgctagtag acaatgccta
     1261 tgtttatcaa gggacactgg cagcaaatga aacacagagt accgtgttac cggaaacagc
     1321 aacaaatgcg accatcacga ttggtgcagc aagcaatgca acgatcaaag cgaatggaga
     1381 atccgtacca gttaatccag gggaaaacaa tcagagtcca aaaaatgtga accttactct
     1441 tcaatacgca gaatgatcgt ataaattagt ttacgcatct gtttacttgt aggtataaaa
     1501 aagaaggatc gtgacaaatc tgtgtcgcgg tccttcttcg ttaattcaaa aagatcaatt
     1561 tcaataaaaa tactcctttg aataaattca tttggaaaag taaataatat cggtggaaac
     1621 ttcgctgata gtcacgtaga atagggaaga aaggagtcga tagtgtgaat ttaccaaata
     1681 aattaactgt acttagaatt tgtatgatcc cgattttcat tttggttgta ggtttacctt
     1741 tagattgggg acaatttaac gtaggagaca ccacactggc tgtcacacag ctagtaggtg
     1801 cagttatttt tgctttagct agtatcacag actggttaga tggaaaaatt gcccgtgcgc
     1861 gaggccttgt cactaatttt ggcaagtttg cggacccgtt ggcagataaa atgctagtta
     1921 tgactgcttt tatcatgttg gttgaccaag gaaaggcccc tggttgggta gtagcgatca
     1981 ttgtctgtcg tgaattagcc gtcacaggct tgcgtttact acttgtcgaa catggagaag
     2041 tcatggctgc agcttggccg ggaaaagtca agacggctac acaaatggtt gctattatct
     2101 tgctgtttat caataatatc ccattcagcc tgatcggtat tccagtagat caaatcatgc
     2161 tttatgcttg tctgatcttt actatctatt caggtgtgga ttacttcatc aaaaacagcg
     2221 gtgtgttcaa aggatcaatg taagtatcgt ttagtggaga ggttgtgagc ctgccgtcta
     2281 actctgagtc acaagggata atttcaaaaa acagcttttc atgttttatg tatattgtga
     2341 cttatgaccg aggttttggc aggagaacac tgtttatttg gaacaaggag ctgtgacaag
     2401 atttttgtca cagctccttg ccctcttttt aaataaaaaa atataaaaat acagcgtata
     2461 ctatataagg gagcgggaag taatgaaagc agaaataata gctgtcggaa cagaactttt
     2521 actaggacaa gtcgtgaata cgaatgccac tttcttgtca gaacagctag ctgatctagg
     2581 tatcgaagtt tattatcaaa cagttgtagg agataatcag cagcgtttag aagagttgat
     2641 tgctctagct gaaacaagaa gtgaactcat actcttatgt ggcggattag gaccgacaga
     2701 agatgacctg acaaaagaag caactgcggc tcacctcggt aagtctctca tacaaaatac
     2761 ggaaggatac aaaaaattat tggcatattt tgaaacaacc catcgaaaaa tgaccaaaaa
     2821 caatctgcaa cagtcccaga ttatcgaagg cggggttcct ctaccaaatc gtacagggct
     2881 tgctttaggg actttttatc aaacagatac acatgcgtat atccttcttc ccggtccgcc
     2941 taatgagttg aaaccaatgt ttgtggaaca agtacgtcct ttactagagg aacgttttcc
     3001 ttctgaagaa aaactaattt ctaaagtttt gcgcttttat gggattggtg aatcgcgact
     3061 ggtcactgag ctaaaagatc tgattgagac acagatcaat ccaacgatcg caccatatgc
     3121 caaaccaaat gaagtgacat tacgactgac agtgaaaaca aatgatgttc aggcagggaa
     3181 tcaagctctt ttagcattag aagaaaaaat ccaagaacga gtaggggaat atttttatgg
     3241 atacggcgat gataattcgc tagcaaaagt cgtagtggaa ctactgaaag aaaacaagca
     3301 gactgtaaca gcagcggaaa gtttgacagc cggcgccttc caagccgcat taggcgacat
     3361 tgctggtgta tctgaagtat ttccaggcgg attcgttacc tactcgctcc aaacaaaagc
     3421 tggttttctg gagattgatc cggagctatt agcagaatat ggaacagtca gcaaagaatg
     3481 tgtggagcag atggccattc aagcacgtga gatagcaaga acagattaca gtgtagcttt
     3541 caccggagtg gctggaccgg acacgttaga aggtcaatct gcaggaacag tatggatcgc
     3601 tatttcatct aaagacggcg tggtttcgga aaaatatcac tttgccagag atcgtgctgc
     3661 tatcagatat aatgcggtta tgcaaggatt tgatcttttg agaaaacagc ttttaagaaa
     3721 atgataaatg cgaatatttg ttcggttttt tcttgctttt tttcactaaa aaagatatga
     3781 taaggataat gaaatgttgc tctgatttgt aaatataagg aggattatcg tattggcaga
     3841 tgatcgtaaa gcagccttag atgctgcact gaaaaaaatt gaaaagagct atggtaaagg
     3901 ctctattatg aaattgggcg aaaaaatcga ccaacaaatc tctacaattc caagtggctc
     3961 tcttgcgtta gatgtcgctt taggtgtcgg cggttacccc cgtggacgta tcatcgaagt
     4021 atatggacct gaaagttcag gtaaaacaac agttgcacta cacgctattg cagaagtaca
     4081 aaaaaatggc ggaacggccg ctttcattga tgctgagcat gcgttagatc cgcaatatgc
     4141 acaaaaatta ggtgtgaata tcgatgaact acttctttca cagcctgaca caggagaaca
     4201 aggtctagag atcgctgatg ctttagtatc aagtggggct gtagatatcg tagtagtcga
     4261 ttcagttgct gctttagttc cacgagcaga aatcgacggc gaaatgggtg actcacatgt
     4321 cgggttacaa gcacgtttga tgtctcaagc attgcgtaaa ctctctggtt cgatcaacaa
     4381 aacaaaaaca atcgctattt tcatcaacca aatccgtgaa aaagtaggcg tgatgtttgg
     4441 gaatccagaa atcactcctg gaggacgcgc attgaaattc tacgcaacga tccgtttgga
     4501 agtacgtcgt gcagaacaat tgaaacaagg tacagatatt gttggtaacc gtacaaagat
     4561 caaagtcgta aaaaacaaag tagcacctcc tttcaaaatc gctgaagtag atgtcatgta
     4621 tggtttaggg atctcacaag aaggagaact tctagacatg gcagtagaaa aagacattgt
     4681 tgataaaagc ggtgcttggt attcttataa agaagatcgg attggtcaag gacgggaaaa
     4741 cgctaaaatc tatatggcta atcatcctga aatgatggca gaagtttctg ctctagttag
     4801 agcagcttat ggtattggtg aagaagtcgc tgtcccagaa gatgaaaaag gacaagaaga
     4861 attgccttta gtagaagaat aaaaattatg ataaacaaaa acggctggtc tgaagagtag
     4921 gtactctcct tgacctgccg tttttttatt gaataaagaa atcgtgagtt atttaattat
     4981 acgagtgggt acataaaata aaaatatctg ttcatctaca aagaataatt taatagaaaa
     5041 gtttctcaat ttaaaggttt acctatatga aactagtgtc atttatttta aaaaccatcg
     5101 tttaatgaat aagtagaaag ccgtttcttt gtttatttaa agttgacact acggggtaca
     5161 aacattagaa taaagttgtg catgtttttt gtacacatat atgcgaagat gcatattgac
     5221 gagtttagaa taatttatca tagaagctgt atagatgata aaaatgaaaa agatagtatg
     5281 gaggtggatt ttatggtatt caacattctc ctcgctatca tcggtttaat tgtcggtctt
     5341 ggtttagggt ttatggtcgc aaagtcacgt cacgagaaag aaatagctgg tgcacaatca
     5401 tcagctgctg gaatcatcga tagtgcaaaa aaagaagcag aaactctaaa aaaagaagca
     5461 ttgttagaag ctaaggaaga aaatcagaaa taccgttctg aagttgaaag tgagctgaga
     5521 gaaagtaagt tagaattaaa atctcaagag aatcgtttga ttcaacgcga acaaacgctg
     5581 aatcgtaaag atgactcgtt agaaaaacgc gagaactcac ttgaaagtaa agaagaagca
     5641 cttagttcaa aacagcaatt aattgaagag cgagaaaaag acgttgaaaa aatgattgaa
     5701 gatcaacaaa aagaactaga aaaaattgct ggtttatcaa aagaggacgc acgagatgtc
     5761 atcatgaaat cgactgaaga agagctgaat catgagttaa ctctaatggt aaaagaatct
     5821 gaacagcgtg ccaaagaaga agcagatcgc aaagcgaaaa acttattatc attagcgatt
     5881 caacgctgcg cagcagatca agtttcagaa acgactgttt cagtcgttac ccttccgaat
     5941 gatgaaatga aaggtcgtat cattggacga gaaggtcgta atattcgaac gcttgagacg
     6001 ctgactggta tcgatttaat catcgatgat acaccggaag cagttgttct ttccggattc
     6061 gatccaatcc gaagagaaat cgcacggatg accttggaaa aactgataca agatggacga
     6121 atccatccag cgcgtatcga agaaatggtc gagaaatctc gtaaagaaat ggatgaacgt
     6181 attcgcgaat acggggaaga ggcagcgttt gaagtcggtg ctcatacact tcatccagat
     6241 ctaatcaaga tcatcggacg tctgcgattc agaactagtt atggacaaaa cgtcttgaag
     6301 cattcggtcg aggttgcaaa attagctggt atcttagcag cagagcttgg cgaagatatc
     6361 caattagcaa aacgtgccgg gttgctccat gatattggga aagcacttga ccatgagatt
     6421 gaaggatctc acgttgagat cggtgcggaa ttagctgcca agtacaaaga aaacccagta
     6481 gtaatcaacg cgatcgcttc tcatcatgga gatgtggaag ctacttctgt tatctctgtc
     6541 cttgtagctg cagcagatgc gctctcagca gcaagacctg gtgcgcgaag tgaatcatta
     6601 gaaaactaca tccgtcgttt aagaagtctg gaaaatattt cgaacagctt cgccggtgta
     6661 gaatcaagtt acgcagttca agctggacgc gaagttcggg taatggtcaa gccagaagaa
     6721 atctcggatt tagacgctgt tcgtttagta cgtgatattc gtaaacgaat cgaagaagaa
     6781 ctggattatc ctggacacat caaagtaatc gttattcggg aaactcgtgc aactgattat
     6841 gctaaataat aaaaaataca aaagaagagc cgcggcaaaa gccacagctc ttcttttttt
     6901 gtaaggcacg aaaaaacatt gctatcatta ttttttattc aacatctcaa gatctttctc
     6961 gatcgtcgta ttgattcctt tgatgattgc tgggatgaat cctgcttctt ctaaggcaac
     7021 tacacctgca acagttgtac cgccaggaga agaaacttgg tctacaagtt cccatggatt
     7081 tttatcactt gaaaggaatt tttgacaaga acccataatt gcttttgcag caatctcagt
     7141 ggctgtctgt ttggacaaac cattcagtac accagtccgt cccatcgcat cgatcagcat
     7201 gtaaacgaat gcgggtgagc tgccagcaag gccaatgaat gtactgaaat ctgattctgc
     7261 taaaggatag acgctgccga ttgcttcaaa aatcatcgta gcttgctgaa gctgttcctc
     7321 cgtggcagct tcatttttgc aaatcgcaga aacgcctgct cctacgctga cattgacatt
     7381 cggcatcaca cgtacgatgg aggcctgagg atcttcaagc gctgtttcta aatctattaa
     7441 tgattttcct gaagcaatag aaatgaataa gactttctgg ctatattctc ttagttcttc
     7501 caatactttt tcaatgattt ttggtttcac ggctaaaaga acgatatctg attgtgatgc
     7561 aacttcctga ttacttttgc atgcatggac gccagtttca gcagcaaatt tttgtgcatt
     7621 ttcaaaatgt ccactggaaa cgaacacctc ttctttcgag actgttcctt tttttaataa
     7681 accatcaata atagcttgtg ccatattccc tgtgccgata aatcctactt tcatgttatc
     7741 ctctccttta gcattaggat atctttcttt ttctatcatc gcaagcaaag aacaggtcta
     7801 aagtggaaaa attaaaataa ataagataac attggggtat gacacaaaag tatacaagtt
     7861 aggaatggga aaatgtgaaa aataaacgat acttgatgct gcaatggata aaggaacata
     7921 gtctgatttt aaaactgatt tttttcggct ccgtgttagt ctttgtggca aatcaagtcg
     7981 caaatattgc taatggcatg agttggcaag atatttggca aaggatggga caacaaagcc
     8041 gcatgacgat cctgctcatg attctggctg gactgctggg tgtcactccg atgttgttgt
     8101 atgattgggt gacgatcaga gtcttagaaa aacaaggaaa accaaaaatg gaaagaaaag
     8161 agtttcttct tgctgcgtgg gtaacaaaca cgatcaacaa tctcgccggt tttggtggtg
     8221 tggtaggagc aagtctgcgg gctagttttt atggaaagca gacaaataga aaaatggttt
     8281 tagccactgt atcaaaagtc gcactattta tgctgactgg tttgtctgta tggtcgtttc
     8341 ttacatttat tgatgtcttt tttattcagt cagaaagtat ttttcgaagc tattggatct
     8401 ggcttttcgg cggtagtttt ttagcaccag tcattttctt ttttgcttat ctaaagagaa
     8461 agacattgtt cttcgagttt tatccaaaag gcttgcttgg tctgctactg gcatcctttg
     8521 gacaatggac gggagccctt gcggtatttt tgatcatcgg caagttgatg agaatagata
     8581 tttcgatgct ttcgatctat cctatgttca tgattgctac attgatagga atgctgacga
     8641 tggtcccagg tggaatgggg acttttgacg ttttgatgat cttaggaatg agtcaattgt
     8701 ctgttcggca agatgtagca gtagtttggc tattatatta tcggttgttt tattatgttc
     8761 taccttttat gacgggactt ctattgttca tcacacatac aggagtcaaa atcaatcgat
     8821 ttttagataa cattccgcga ctgtttttcc aaaaggttgc gcatatcgtg ctcgtaagtg
     8881 ctgtctattt tgcggggatc atgatggtac ttctatcaac aatcactaat ctgtctgcaa
     8941 tcagtcggct atttcaagta ttattgccgt tctcattcaa ttttttagat cagacagtga
     9001 atctattggt tggcttttta ttactcggcc ttgctagagc actttcagta aaagtcaaaa
     9061 aggcatttct gcctacgatt gctttactat cttttggtat tttaaataca atcacacgta
     9121 cgttatcttg gcagttgata ttggtatatg tcttgattct gttagctgtg tggttatcca
     9181 gaaaagaatt ttatcgaaca aaattcgttt attcttgggg agctataatc tttgatgcta
     9241 gcttgttcgg ctttttgttt atcgtttacg caattgcagg ctatcacagc ggacgctttt
     9301 tagtcggtcg aataacagga aatcatttta ttttgtttcc ttcagatgat gtgtggttct
     9361 cagggcttat tggtttgggc atctcattgc tagctttagt ggcgctttat caatatttag
     9421 ctgaagatac tacgaagctt ggcagtgagt acgaaaaaga ccgttttgac cgtttattgg
     9481 aaacatacgg aggcacagct acaagccatc agcttcgctt gtcaggttac agttattttt
     9541 attaccaaga agacgaccaa gatcaagtcg tttttggtta tcagatcaaa ggaaacaaat
     9601 gctttgtttt aggagatcca gttggcaatc ctaaaatgat gcgggcagca acgaatcagt
     9661 ttatgaaaca agcagattgt ttaggctatc agcttgcgtt ttataagata tcagaaagct
     9721 acgtagtgat gcttcatgaa ttgggttttc acttcacgaa agtaggtgaa gcagggattg
     9781 ttgatctagc aacagaagac ttgtctcggc tgagtcagca acttgaatat caacgtttga
     9841 tgaaagaagg ataccatttt acttggtacg atcaactgcc ggaagaatta catcaagctg
     9901 taaatgaagt ttcacaaaaa tggcttcaag gtgcacgaga aaaatacttc tctgtgggac
     9961 gatttgataa atcatatgta ttatctagcg gtgtcggtat tgtccgtaaa gatcaaaaag
    10021 tcgttggctt catcaccgct cagccgatga cgaagaagca ggcgggatat gatttgttgc
    10081 gggtcttacc taaagaatct gaggaattag ctgactatct gttagccaat ctgtttattg
    10141 tatacgagaa aatgggctac tcggaagcca atctaggctt agctccatta gctaatgttg
    10201 gagaaacaga tttttctttc tttcaagaaa aagtcatgca tatcgtatat aattatggta
    10261 attttttcta ttccttccag tctgcttatg aaaagaaatt aaaatatgtc gatcattggg
    10321 aaggacgtta ttttgcttat aggaaaggaa gtaatttcgg atttgctacc atgcagctac
    10381 tcagtctgat aggaaaagaa aaaggaaatg ttccgagttt ggcagaagaa gtgctcacag
    10441 aattataaga atcactagaa cttgtttgga ttatgaagag aaaattctgt gaaagctgtt
    10501 gaaacgatga caaaatcaac cactaggcag acataagcta tacttttatt tttccctatg
    10561 atagaatact tgcgacttag acttttcgaa tgggtgagag gatggaattg attgaaaaag
    10621 cgccagccaa gataaattta ggactggatg tattatatag acggcaagat gggtatcatg
    10681 agttggaaat ggtgatgtcg agcgtagact tggcagatca tctatttttt gaagaaataa
    10741 cagaagacaa aatcattgta gaaacaaata aagcattttt accggtagat gaacggaaca
    10801 atgtttatca agctgcttta ttgctaaaaa ataaatacaa tatcaaaacc ggtgtcaaga
    10861 tctttgttaa gaagaatatt ccagtagcag ctggattagg cggaggaagt acagactgtg
    10921 cagctgctct aagagggatc aaccaattat ggaatctggg ctgttcgtta gaagaactgg
    10981 cagaaatcgg tttggaagtc ggtacagatg ttccttactg tgtctacgga agtacggcat
    11041 ttgtcggtgg caaaggggaa gaaatccaag tattaccttc tatgccacag tgctgggttg
    11101 tgttggtcaa accaagaatg agtgtctcta cacgtacagt gtttagagaa gtagatacgc
    11161 aagccatcca acatcaagac atccacggac ttgctgaagc catccgagag caagattatc
    11221 agcagatgat caaaaaaatg ggaaacagtt tagaagatat aacgggaaag cggcatccag
    11281 tcatccatca aatcaaagag cgtatgatca agtacggagc agatgctgca ttgatgagtg
    11341 gaagcggacc aaccgttttt gccttgtgtc aacattattc aagagcgcag agagtatata
    11401 atgggttaaa aggtttttgt gaggaagttt atttagtacg aaccctgcaa taaaatataa
    11461 gaagtaaaat aggtgatgaa aaactgagga atcctaaaat ggtattcttc agttttttat
    11521 tgtagtgaaa tggctagata tgagcagcaa ctcatccatt ttacgattta atcaagaata
    11581 tacatgctta aatcagaaat tgatggattt ctattgactg tgttttttta ttttgataca
    11641 ttagttcacg ttaataaatc gtaaaattcc gatttgttca ttgaaaggag catataaatg
    11701 aaaaaatatc ttttgacagc tacggtaatg ataggtgctt tactgtttgc agcatgtgga
    11761 aatacgaaca aggaagcaga caaaaaagag gatttgacga tcgttacgac tttttatccg
    11821 atgtatgatt ttacaaaaga gatcgtaggg gatgaaggaa acgtgaaact tttgatccct
    11881 gctggaacgg agccgcatga tttcgaacca agtgcaaaag aaagagcaga aatttccgat
    11941 gcagatgttt tcgtgtataa tagttcagac atggaatttt ttgtggattc gttaaaggat
    12001 tcagtggata gcaaacagac cttgatgatc gaagcagcta aaggaatcga ccgtttagaa
    12061 tcgcaagaag cagacgaaca tgaagaatca gaagaagggc atggtcattc acatgaatac
    12121 gatccacatg tttggctcga tccggtatta gctataaaag aagtcagaac gattgctgaa
    12181 gaattaggcg aaaaatatcc agataaaaag gaaactttta cgaagaatgc cgatgcttat
    12241 atcaaaaagc ttgaagcact ggatcaaaaa tactccgatg agctaaaaga tgcaacaaac
    12301 cgcacatttg tcactcagca tgctgctttt gcttatttag cgaatcaata cgacttaaaa
    12361 caagtagcga tttcgggcgt ttcaccagat caggaaccca caccaagtcg cttagctgag
    12421 ctaaaagaat ttgtgaagaa aaacaacatc aaagtgattt attttgaaga aaatgcctct
    12481 tcgaaggtag ctgagacatt gtcgaatgaa acaggagtga agctagaagt gttgaatcct
    12541 ttagaaagct tgactaatga acaaatcaaa gcaggcgaaa attatatttc tgtcatggaa
    12601 aaaaatctga aagctttaaa agaaagtatc aattaagttt tagaatggag gttgtaaggt
    12661 gcgttatatc gaaatatctg atttgacctt ttactatgat gatgaacctg tactagaaga
    12721 tgtctcttat catgtagatg cgggtgaatt tgtcatttta actggagaaa atggagcagc
    12781 caagtcaacc ttggtgaaag catcgttagg attactgaaa ccttcaaaag gaatgataaa
    12841 gattgctgag aaaaatcaag aaggaaaaaa attaagtatc ggttatattc ctcaacaagt
    12901 tgcatctttc aatgcaggtt ttcctagtac ggtgatcgaa ttagtccgtt caggaagata
    12961 tccaagaggc cgctggttca agcgtctctc tgaaaaagat catcaacatg tacagcgtgc
    13021 tttagaagct gtagggatgt gggatatgcg aaatcggcga ataggtgaat tgtctggcgg
    13081 acaaaagcag cgaatcagtt tagcgcgtat ttttgctact gaccctgact tatttgttct
    13141 tgatgaacca acaacaggta tggatgaaga atccagaaat gaattttatc gtcttctccg
    13201 acatagtgcg catgtccatg ggaaatcagt tctaatgatc acccatgatc atgaagatat
    13261 caaacagtat gcggacagac agatccgact ggtgagaaag gaggattctc aatggcgttg
    13321 ctttcatatg aattcatgag aagagcgttt cttgccgcta tcttcattgc cggaatcgcc
    13381 ccgatgctag gtgtgttttt agtgatcaga agacaatcgc tgatggctga tacgctttct
    13441 catgtctctt tagcaggggt agcgttagga ttctttttga accttaatcc tacacttacg
    13501 accatgctag tcgttgtatt agcagctgtg atattggagt atctgcgaac gatctaccga
    13561 acctattcag aaatctccat tgccatttta atggctggtg gtttggcgct agcgttagta
    13621 ttgatgaatt tgagcggagg gaattcagca acaagtatcc aatcctatct gtttggttcg
    13681 atcgtgacca ttacatggga gcaagtcatc tttttaggaa tcttattcgt tgccatcggt
    13741 atattgttct ttttattcaa acggccgatg tatgttctga cttttgatga agataccgcc
    13801 cacgtggatg ggctacccgt tcatttgatg tcgatgatgt tcaatgtgat tactggtgtg
    13861 gctattgctg tgatgatccc gattgccggt gcacttttga tatccgcaat catggttctt
    13921 cccgcagcaa tcggtatgcg attaggcaaa agcttcaatg tcgtgattgc tatcagtgtt
    13981 tgtgtaggat ttctagggat gctgtcagga ctaactagct cgttttatct agatacacct
    14041 ccaggagcaa caatcacatt agtctttatt gctttgttcc tgattgtaaa tatcatcaaa
    14101 cgaatattgg ttgccttcag aagaaaaaat aactaaatta taaagaaaag aagccttata
    14161 aaaacgaaaa attttttata ggcttctttt tattttaata aataccgaac tattttctta
    14221 ttttttcata aaatgctcga atttttgaag aaaaacttat ataatgtaaa acgataaaat
    14281 ttcttaaaag gagttggaat aggtgaaagt acgtcgcagt gaacgtctag ttgacatgac
    14341 acagtattta ttgaatcacc cgcatgaatt gatttcctta acaagctttg ccgaacgtta
    14401 tgaatctgct aaatcttcta tcagtgaaga tttgggaatc atcaagaaaa catttaaaga
    14461 acgagggtat ggaaccttgg aaacaatccc gggtgctgca ggtggtgtat tgttcatccc
    14521 tgaaatttca tatgaagagg cgaaaaaata tattgaatct ctagcagaac gtctatcaga
    14581 acaagaccgt ctgcttccag gaggatatgt ttatctctct gatttgttag gagaacctga
    14641 actgctgcgt caagttggaa gaatcattgc ttctaaatac cttggtgaaa aaatcgatgc
    14701 tgtaatgact gtagccacaa aaggcgtacc tattgcccaa gctgtgtcct attatctgaa
    14761 tgcgccattc gttattgtac gccgggactc aaaaataaca gaaggatcaa cagtaagtgt
    14821 caactatgtg tctggatctt ctgaaagaat tgaaaagatg gaactgtcta agagaagttt
    14881 gaaacgtggt tcacgtgtcc ttgtagtgga tgatttcatg aaaggcggcg gaacagtcaa
    14941 cggaatgaag agcttgattg aagaattcga atcagaactt gttggcatca ctgtatttgc
    15001 cgaatcaaaa ttcaacggtc atcgcgccat cgatgattat acgtcactgc tttatgtcaa
    15061 agatgtagat actcaaacga aaaatattac ggttgtgcca ggaaattatt tttcagaata
    15121 agaggttgtg aaaaagccgc ttagtttcga gtagtaagaa aaattttgtg aaaatggctt
    15181 tccacatttt ttcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttat
    15241 ctagaggttg tgaaatcagc gtcctgtcct gagtattagc tcaataaacg gaaaaattgc
    15301 tcctcatatt ttgccgttta ttgagctaat accgaaggtc agctgattga aaaacgttta
    15361 atgaagaggt tgtaagccta ccgtttaact ctaagtcaca agaaacgatt tcaaaaaaca
    15421 gcttttcatg ttttatgagt agtgtgactt gtgaccgagg agttggcagg agagcaccgg
    15481 ttaccaaata gataggcccg ttatggtcta tctatttttt tatccagaaa acgctgggat
    15541 aaaagaaaat cacttgtttt gttgaaaaaa tgggtatact gggataaact gaaatcagca
    15601 aactatattt aggagtgacg cagttggacg cacgttatgc aattattttg gcggcaggga
    15661 aaggtacccg catgaagtca aaattatata aagttttaca tcccgtttct ggtaaaccta
    15721 tggtagaaca tatcattaat cgggtcagtg aaacgaaacc agatgaagtg attacgatcg
    15781 ttggacacgg agcagaacaa gttaaagcac aactaggaga acgcagtaaa tatgcgttac
    15841 aagcagaaca attgggaaca ggacatgctg ttttgcaagc tgcttctttc ttggaaggaa
    15901 aaaaaggaac aacgttagtt atcagtggcg atacaccatt attgactact gaaacgctga
    15961 acaatctttt tgaatatcac caaggaaaaa atgcaagtgc aacgatcttg acggcacaag
    16021 cagaaaaccc aacaggatat ggacgaatca tccgtgacca tattgggatc gtagaaaaaa
    16081 tcgtggaaca aaaagatgca acaccagaag aagctcttgt acaagaaatc aatacaggaa
    16141 cttactgttt tgacaacgaa gctttgttcg atgcgttaag caaagttgga acaaacaatg
    16201 ctcaaggtga atactatcta acagatatca tcgaaatctt aaaagaagaa ggccatacag
    16261 ttgctgctta tcaaacggat gattttgaag aatcaatggg agtcaatgat cggatcgcgt
    16321 tagctaaagc aaatgagatc atgagaaagc gaattaacca gatgcatatg gtcaatggcg
    16381 taagctttgt cgattcagca acgacataca tcgatgcagg cgtagaaatc ggtccagaca
    16441 cattgatcga agcaggtgta caaatccaag gaaatacagt cattggttct gattgtgtca
    16501 tcgggtcaca ttcaaaaatc gtagacagta ggattgagga tcacgtcgtc attgaaaact
    16561 cagtcattga aagcagccat gtcaagaaac atgcagatgt gggaccttat gcacatttac
    16621 gaccaaaagc tgaaattggc gaaaacgtcc atatcggaaa ctttgtggaa gtgaaaaatg
    16681 ctcaaatcgg caaaggaact aaagtcggtc acttgactta tgttggtgat gctacgctgg
    16741 gagaagaaat caatgtcgga tgtggtgttg tcttcgtcaa ctatgatgga aagaacaaac
    16801 atcatacgac tgtcggcgac cacagcttta ttggttctag cacgaatatc atcggacctg
    16861 tcgaggtagc taaaaacagt tcgattgctg ccgggtcaac aattacagac aatattccag
    16921 aatatgcgct ggcaattgcg agagcaagac aagtcaataa agaaggatat gcaaaaaaac
    16981 tcccttatct aaactaagac ttttgcaaaa tcacttgatt tatttaaaaa atatgactac
    17041 tatttattag gtagaattct agagttgtga aaaagaaagt ggaggtcccc atgtcaaaac
    17101 attactttga cccaagatta aaaatttttg cgttaaattc caatcgccca ttagcagaga
    17161 aaattgcagc agcagtcggc gtcgagttag gaaaatcatc tgttactcaa tttagtgatg
    17221 gagaaattca agtaaacatt gaagaaagta ttcgtggatc gcatgtctat gttattcaat
    17281 caacgagcag cccagtcaat gataacttga tggagttgct gatcatgatt gacgcactga
    17341 aacgagcaag tgcaaaaaca atcaacgtag tcatgcctta ttatggctat gcgcgtcaag
    17401 accgtaaagc acgtgcacgg gaaccaatca cagcgaaatt agtagcaaac atgattcaaa
    17461 aagctggtgc aacaagaatg ctgactttgg accttcatgc agtccaaatc caaggtttct
    17521 ttgatattcc agtcgatcat ttgatgggtg cgccacttat tgccaattat ttcttagaaa
    17581 aaggaatcaa aggcgatgat gttgtcgttg tttcaccaga ccatggtggt gtcacacggg
    17641 cacgtaaatt agcggaattt ttgaaatctc cgattgcgat catagacaaa cgccgtccaa
    17701 aagcaaatgt tgcagaagta atgaatatca ttggacaagt caaaggcaaa acttgtgtac
    17761 tgatcgatga tatgatcgat acagcaggta cgatcacatt ggctgccaat gctttgaaag
    17821 aagctggtgc aactagtgtg tatgcttcat gtacacatcc agtattatcc ggccctgctt
    17881 tacaaagaat agaagattca gctatcgagc gtctagttgt tacagactcg atctatcttc
    17941 cagaagatcg taaaatcgac aaaatcgatg aagtaagtgt tggcggacta atgggagatg
    18001 caatcaaacg tatccatgaa aacaaaccag ttagtccatt gtttgaaaca aaaaataaat
    18061 aataaataaa ctcacaaagg ggagtccaat tgggctgaga ttgaatctat ttctaaaccc
    18121 ttcgtacctg tatcggttat gcgagcgtag gaattgtgaa taaacagctg tttgactatt
    18181 ttcgaaagct gcttcctcct ttgtgacttt tttattcaaa ggaggaagtt tttttatggg
    18241 aaaaaataga gtttgggtag aaggaacgat tgttgcagcg ttagcgatgg tgttgtcgtt
    18301 gattccgatc cagatcggca gcagtttttc tatttcatta gggcagattc cattgactct
    18361 ttttgcgtta agaagaggga caaaggcagg gatgctggca ggtttcattt ggggcatcct
    18421 acattttccg ttgggacaag tttattattt aagtgtcgtt caagtgttga ttgagtaccc
    18481 gattgcctat acttttgcag gcgcagcagg gctcatggct tcaaatgtcc aaaaaggcta
    18541 tgtagacgat aatacgaaac aaattagaaa aagcatcgtg tttggtgcat tgattggcgc
    18601 actttccaga tatttttggc attttattgc aggagtggtt ttctggggag cgtatgcact
    18661 atgggggatg aatccttggc tcttttcttt cgtaatgaat ggtgccagcg ggttgagtac
    18721 agctattgta acgatcatcg ttttattagg gatccaacaa acgactcctt cgatttttgt
    18781 gccagataaa cgacaggagc tgtaaataaa aaagaagcaa gaaacttgag acagaatcat
    18841 gtcctagttt cttgtttcct ttttattttt tgataagaaa tagatctact tcaatggcag
    18901 ataagaaaga tcggcatctt ctaccaattg ccagtgtcga tcggtgacag agtagattaa
    18961 agtagcgatg atttcctctt ctcgataccg ggtgcctttt aatttccaaa tataatcttc
    19021 aatcacatag attgggatac gatatttacc atcgatcaaa ccaacttgat caaaagaaaa
    19081 aatgatccct tcctgcatca aattacgcat ttctttcaat aattccacac tgattttttc
    19141 aacaggtgtt tcttttttga aaaaagattt ttttacattg atcttatctg acaagatcga
    19201 acttaattta ctagcaaaaa tcctataaaa ttcatctaaa tagcgataat aaatccgatt
    19261 cagctgatca ttaagatcga agtatacaaa attattctgt agtttataaa aaaacggcga
    19321 atgaagatgt gttttcatat gaccaaaata taataattct gaaatttcta aaggtgtcaa
    19381 ttctttcagc atcgttaaat cagtaaagtc gatccacttc aattcatcga atccacgcct
    19441 gttacgagaa gaggaaaaga aacgctgaat attttctgta ccacggatta ctttcagccc
    19501 tgtatgggat tcatattctc cagcagaagc agatggatca agcaagagca agttttgagg
    19561 aaaatgcacg atactgtttg caaagtcagt agcagtcagc ccttttgtta aaacagcatt
    19621 acttgtggta tcaatatgta cataaactga ctcagtcatc atgttcctcc tcttatttcc
    19681 tcttaatctc ttttatttta caacatttgc attaaaaaag cttgtttctt ttattttaaa
    19741 attccattac aaaatcacgt tttcattgaa aacactctca ttttcaatta atttttgtga
    19801 tttttgtatt tttttattta aaggaggttt tatgaattgt tttcttatgg aatctacaaa
    19861 gtcgaatacg agactcatga cctttttatg acgtactggt atacttgaag aaaaaggaga
    19921 gcgcgatgga gataaaaaaa gtttactttt cacagaaaat gtcgctgtat tcgcctatcc
    19981 agcaagcaaa acaacccctt ccattttttc aaagtcatca agaaagtaga gacgcttttt
    20041 ggcgttctgc aaaaaagcaa tgtgctttta aagcagtatc gaaaaaatat accgtctatt
    20101 ttactattgc taaaagtaga ggagatgtta tttttaataa tctgttgatt gaaggtgaaa
    20161 cttttgaatg gaaaaaattt tttcggtata tggaagcttg cgctcgtttt tttattaaag
    20221 aaaactgttg ttttttattt caatctcctc tatcagaaga atggttgcgt atttttcata
    20281 aaaacggcta tcaaggaacc acacaattaa ataaaaagct ggtctatcat acagcacttg
    20341 ttttaggtgg aggaggagcg cacggtgctt atcagattgg tgtgtggcag gcgctgaagg
    20401 aacatgatat caactttgag attatcacag gtacctcggt aggtgcgttg aacggcgcat
    20461 tgattttaca aggagatatg aaaaaagctg tgaatttatg gaaaaagctg tcgacaagac
    20521 aagtgcttgc gttaccagaa atggctgcag ttgaagacct tcgtgaacgc ttctatcgag
    20581 aaagacgcca aatgacgaaa acagcactaa ttgaaggcgg ggtttcttct gctccattgg
    20641 aaaagatggt caaagacaat ttggatttgt ccttactcaa acataatccc aaaatacgtt
    20701 tatatacggt gtctactaaa ctgccagaac tagcggaagt agtgactgat atccaacaga
    20761 caaaaacaga agaaatacca gattggatac tcgcctctgc ttcattttat cccgctatgg
    20821 cttatcgaaa gatcggaaaa aataaatatg cagacggtgg gtatcgcaac aaaataccta
    20881 ttgatattgc gattaacaaa ggagcaacag aggcatttgt cgtagatgtg caagggccag
    20941 gcccagcgaa aaaaattcgt atgccagaca tttttatcca ttggaaatgt caaacattgt
    21001 ggacactagg cagctttctg ctttttgata gccaaaggaa ccaattaaat ttacaattag
    21061 gctatctgga aatgaaaaaa cgattaggct attactatgg gaactggtat acatttcatt
    21121 ctgttaaacc tgcaagaaca tactggcgag gatttttgtc ttacttaaca aaagaaattc
    21181 agttagaatt atcttttttc aaaagcataa aattttggca aaaactgcgt aatttatata
    21241 agaacagggt cgtaccagag acatgcggat tggcgatgct agaactttta gcaaaaaaac
    21301 attttttact gcccaatgaa atctatcaag tagacatgat gattcaaatg atctgccaac
    21361 aagatataaa aagtatgccg gatgatttat tggcacaaat tggtcaatta agtactgaag
    21421 aatggcgaag gtatcgaaaa taccagcaaa aaatacaaaa tgagcgcacg aaaactgttt
    21481 attttgatta tctgctacaa ggaaaacgca aggatcgttt gcagcatgaa ttgcttaagc
    21541 aaccagttga cacattactg atcctatatt tatattatct caaggaggaa caaccatggc
    21601 acaagaattt tcatatgaaa tcgtagaaga aatcgctata ttgtcagaaa acaacaaggg
    21661 gtggcgtaaa gaactgaacc tagtcagctg gaatggtcgc ccgccaaaat tcgatcttcg
    21721 agattgggcg ccagaccatg aaaaaatggg gaaaggcctg acattgacga acgaagagtt
    21781 tgaacaactg caaaaagcaa tagaaaatat gtaatgagga aggaccctgt cgaatgaaaa
    21841 gtatgactgg ttttggcaaa gccatccgcg aaacaaaagc gtatcagtta gatattgaaa
    21901 taaaaagtgt caaccagcgt tttctagatg ttcaaatacg aagtcctaaa atcctgaact
    21961 ttgttgaaaa cgattttcgt cagcttatta aacagcagtt aagccgcggt cgaatcgaag
    22021 tatttgtgaa tctagcttat attggcaaaa acgaaaaaca gatcgtcatc aactgggaat
    22081 tgattgattc acttatcact caattgacag acggaattca acaacgatac ggtagtcaaa
    22141 atcaagtgtc aattagtcgg atggcggaac gcttcgtgct ggacgaaaac ttcgtaacgg
    22201 tagaagaaaa accggaagag agcatggaag aattggcaca gttagctcta gatacattga
    22261 aaagcgcact tgattctatt gaggcgagtc gagaaaaaga aggtcaagct ttagctttag
    22321 tgttgaaaaa gaatactgaa gaattcaaag aagtactaaa aaaactcgat agttttgtag
    22381 aaatctatga aaaggaattt caaataaggt atcaaaaaaa gctggaggaa tttcttggag
    22441 cgaaagtcga tgagcaacgg ttgttgacgg agctagccat cttgttggaa cgcggagata
    22501 ttcacgagga actggatcgt ctggcgatcc atgtacaaaa actagatgaa cttatcgaag
    22561 cacaatgtcc tgtaggtcgg gaattagatt tcctgatcca agaaatgaat cgggaaatca
    22621 acacgatcgg ttccaaatca aatgcaatcg aaattaaaaa tcaagtcgtc cagtctaaga
    22681 cgatcttaga aaaaatcaga gaacaagtac aaaatattga atgacgaaag aggtagagat
    22741 aatgaaatat tatgacgtaa cgttccacga attaagtgga aaaactgtgg ttaaacggga
    22801 tatcccttct gaaaaaaacg gctttgacgt atggaaagat gcctgtgcag actataatga
    22861 gaacgaactg tttattttga tcaacgacgg tgcttacgtg acgatgaatc ggaaatttat
    22921 tgttcgaatt gacacagaag aggtagaaga tccgacagaa aaagcacgtt ctcgaaaaga
    22981 tgagatcatg ggtgtagtga atactttgtc caatatgggc ttttagcaag gatgctgaac
    23041 aaagaaatac aataaataaa cagaattgga tttcttcgct tagcttcttt gctctaggaa
    23101 tacatttaaa ggttgtgaca aaattcgtgt cacaatcttt ttttattttg aataagtgat
    23161 gttcaaaaac cattttctcg gtattaagcc aaatttatgc aaaaatgtga gaaactattt
    23221 tcacaaaatt tttcttcata ctcgaaacta atcggcttct tcacgacctc ttatacacgg
    23281 cgttcaaaag cttgcatctg cgataataac cccaaccaaa cgaaaaacat gagaagctgt
    23341 ttatgtttgg ctgttcttat tacttgatgc aggacagctt ttttacgacc tcttggtaca
    23401 gggtagcttt tttaccaatt tctagctata attaaagata accttgaaag ttgcagaaaa
    23461 aaagtgcaaa ataaacgata agtacaaaaa gactagttga aaggacgtcg tcatgtcaga
    23521 gcgtggatta ttaatcgtgt tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa
    23581 agcaattttt gagagtgagg ataatgattt tcagtattcg atctcaatga ctacccgtaa
    23641 aatgcgtgaa ggggaggtcg aaggcgttga ttatttcttc cgaagcaaag aagagtttga
    23701 atcaatgatc gaagctggag agatgctgga atacgcagaa tatgtcggga attattatgg
    23761 aactcctttg tcatatgtca acaagacatt ggatgaagga aaagatgttt tcttggagat
    23821 tgaagtccaa ggagcaaaac aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt
    23881 gactccgcca gatttagcgg aattaaaatc acggatcgta ggccgaggaa cagatgcaga
    23941 tgaagtaatc gaaaaacgaa tgaaagttgc aaaagaagaa atcgaaatga tggctcttta
    24001 cgactatgct gttgtaaatg atgaagtgcc gctagcagtt gaacgaatca aaaacattat
    24061 tgccagtgaa cattttcgag tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga
    24121 gatgtagtct tatgatgtta aaaccttcaa ttgactcact attagatcgt gtaaattcaa
    24181 aatattctct agtgatttta gcaagtaaac gcgcgcatga attggatgca agtgcacagc
    24241 ctactctaga ttcttttgat tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg
    24301 caggaaatgt agtcaatgat ccacatcctg aattaaaacg tgaacgtttg aagatggaag
    24361 aagaggaacg tcaagcacaa aaagagcgtg aacaacacga attggaatca cgtatccgtg
    24421 aagagcaaaa aatgtaataa agaaaaatag agcgaacatc taaaaagaaa agcaaaccca
    24481 gctgtttcct tttcaaacat tcgccactta ttgtctggta gaaaaggctg tgtctgaact
    24541 ttttgacaca gttctttttt ttcttatctg ctgttaattt tttgttagga tttggcagaa
    24601 gataagacaa atccatcgat ctctagtaaa ataggcaagg aactttttgt cgcttgataa
    24661 gaaaagacaa aaaaaggagg aaacgaacca tgacattaac agctgaagtc attgtggacg
    24721 tacctactat gcagaccgat cagccattca cttatttggt tccctctgaa atggaaacag
    24781 ctttgcaagt cgggatgcga gtagaagtcc cttttggtaa tggcaatcgt catgtccaag
    24841 gatttgttat ggctatccaa aaatcagaag aatcagctaa tccatctttg aaggcagtta
    24901 ttcgtttgct agaccttgct ccggtggtca atgaagaatt actttcatta gctgattata
    24961 tgaaaaaaat tacttacgca ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga
    25021 tgaaagcaga atatgataaa ttgatctatc ctttggcaga cacaccagaa gttcaggtat
    25081 tgtttgatca gcgagaagtg atttcctgga aggaagcaga agaagaaggc agcctttctc
    25141 aattgattcg ctggcgccaa gagcaattag tagatatcaa atatgaagtc catacacgaa
    25201 ataaagtaaa aacgattcgt ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag
    25261 aatgggcaaa attacggcaa aatgccaaga aacaaaaaga acttcttttg tgtttatctg
    25321 aactgagcca agaagaacca attgcttatt tcaaaaataa aggaatcagt actgctgtct
    25381 tgaatcaagg aaaagaaaag ggctggctgg agtttataga atcagaacgt tatcgcgacc
    25441 cttataaaga ccgagtattt gaccaaacga cggcattgga actgaatgca gaacaaaaaa
    25501 atgctgtcga acaaatcatc acagcaggtc agcagcaaaa agatcaggtc tttttgctgg
    25561 agggaatcac tggcagcggg aaaacagaag tatatcttca ggcaatcgct gatgttttgt
    25621 cagaaaataa aacggctatt atgttagtgc ctgaaatcgc attgactccg caaatggtcg
    25681 agcgattcaa aggacgcttc ggagaatcag ttgcggtctt acatagcgga ctttcccaag
    25741 gtgaaaaata tgatgaatgg cgcaagattg agcgggaaga agcgcaagta gtagtaggcg
    25801 cacgatctgc tatttttgcc ccgctgaaaa atatcgggct gatcattatc gatgaagaac
    25861 atgaatcaag ttacaaacaa gatgagacac cgcgttatca tgcgagagat ctagcaattt
    25921 ggcgcagtaa atatcatcat tgcccaatag ttctaggaag cgcgacgccc tctttagaat
    25981 caagagcacg agctcaaaaa ggcgtttatc agttattgca gttaaaccac cgagccaaag
    26041 cagctgcaca gcttccagct attgagatcg tagatatgcg tgaagagttt cagaatcatc
    26101 ggacctctac tttttctgct aatttgcagg aaaaaataca aaatcgatta gacaaaaaag
    26161 aacaaactgt cctactgctc aatcgtcgag gatattcgtc ttttgtgatg tgtagggact
    26221 gtggatttgt tcttccatgt cctaattgcg atatctctct tacactgcat atggatacac
    26281 gttcaatgcg ctgccattat tgcggacatg aagaacctat tcccaatcgc tgtcctaatt
    26341 gtggcggcaa taaaatccgc tactatggaa caggtactca aaaagtagaa gaggaactcc
    26401 gagaacttta tccgcaagct aggattttgc gtatggatgt agataccact cgaagaaaag
    26461 gggcacacga acagatttta caaaaattcg gtgcaaaaga agcagatata ttattaggga
    26521 cacaaatgat tgctaaaggt ttggactttc cagaagtgac attagttggg gtgttgaatg
    26581 ctgatacctc cctgaattta ccggattttc gttctagtga gcatacattc caattgctta
    26641 cccaagtttc aggacgagca gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat
    26701 tcaatcctca acattacgcc atagaattgg ctaaaaaaca aaattatgag cagttttatc
    26761 agcaggaaat gcatgtacgg catcgtggag gctatcctcc ttattacttt actgtgaaaa
    26821 taaccgccag ccatcctgaa gaacaagtag cggcaaagaa aatctttcag attgccaatc
    26881 agctaaaaga agtgttaagc cctcaaagct tgctattagg gccaacgccc agcatgattt
    26941 tgagagtgaa aaaccgatat tattatcaac taatcatcaa atataaacat gaaccgaatc
    27001 ttcctcatgt tttggatgaa attttgaatg gaagccagaa agaacagcga caaggtcttt
    27061 ttgtggcaat tgacaacgaa ccattaaact ttatttaaga aagggccaaa ttatgcgcta
    27121 tcctatattg atccacccta atgataaatt aaaaagaact gctcagccaa tcgatgtaat
    27181 cacggacgag acaatcgctt tattagacaa tttatatgag acaatgattg cgaatgacgg
    27241 aatagggatc gctgctcctc aagtaggaca aaacaagaga attgctgtga tcgaagtaga
    27301 tgaaggcgag aaatttgaac tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct
    27361 tgacgtagaa ggttgtctca gtattccgca tgtctatgga acggtcaagc gagcagatga
    27421 agtgaccgtg cgttattatg atcgagacgg agaagagata gaagtgacgg cttttggcta
    27481 tttagcccgt gccttccagc atgaaatcga tcacttagac ggtatcttgt tcattgagaa
    27541 aatgatacag cagatcccgg aagaagaact tgaagaatat atggaggaac atcttgatga
    27601 ctaagctagt atttatgggc acacctgctt tttctgtgcc gattcttgaa gggctgatag
    27661 aatcaggtta cgagatccag gcagtggtaa cacagcccga ccgtccagtt gggcggaaac
    27721 gagtaattac gccgacaccg gtaaaaaaag cggctctgaa gcacggtatc cgtgtgctgc
    27781 agccagagaa aatctccggt tcgccagaaa tggaagaaat cattgaattg gtgcctgatg
    27841 tgatcgtcac tgcggcattt ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat
    27901 taggggcaat caatgtccat gcttctttac ttcctaaata tcggggaggt gcgcctgttc
    27961 attatgccat catgaatggg gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa
    28021 aaatggatgc aggcggaatc tatgcgcaag aaagcatacc aatcactaaa caagatgatg
    28081 tagggacgat gtttgaaaag cttagccttt taggaagaaa gctattgcta gaaacattgc
    28141 ccaatatttt ggatggacaa aaaccagtac ctcaagacga gtcagaagca actttttcgc
    28201 caaacatcac acgggaacaa gaagcaatcg attggcataa aactgcagaa gaaattgaca
    28261 accaagtacg cggaatgcgt ccttggccaa ttgcattcac gacctatgag ggcatacgct
    28321 ggaaattatt atcggttgaa gtattggcag aaactacaga caaagcacct ggtacgatca
    28381 tccggaaaga caaaaaaaat ctatggattg cttgcggtaa acagaccgtc ttagccatca
    28441 atgaattaca gccagctggt aaaggaaaac aggccgtaca tgatttcctt aacggaagcg
    28501 gtcagcaagt tcagattggt cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa
    28561 aaaatcagta cgatatatgg cattactgac attagagcga gtgaacaagg gtggcgccta
    28621 ctcgaatctt ttgttgaatg agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg
    28681 tttatttaca gaattgatct atggtacgat tagccatcag cgactattgg aattttacgt
    28741 tgcaccattg atcgctaaag caaaaaaagt ggatgactgg gtcaagatgc ttctttatct
    28801 ttctgtatac caattagaat ttttagataa agtccctgca catgctattt taaacgaggc
    28861 ggtcgaaatc gcaaaagtaa aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt
    28921 gaggaattat cagcgtcaag gagcaccaga tctgcaaggc attgctgatc ctatcgaaag
    28981 actttctgtc aaaatcagcc tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg
    29041 atacgaagaa actgaaaagc tcggtttatc gctatatcag cctagccatg ctagtgcccg
    29101 agtagataca cgccgacttt ccagagatca ggccatcact gttttgcaag aagaagagat
    29161 cacagctgaa aaaagccaag tcagtccata cggtatagta gcgaaaaaag ggcatttagc
    29221 ttctagctca ttgttccacg atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt
    29281 agcgccgtct atgcaaatcg agcctgaaca tcatgtctta gatgcctgtg cagcacccgg
    29341 aggtaagacg acgcatattg cttctttttt agatccgtca tgcggtggag aagtggtcgc
    29401 tttagatgta cacgaacaca aagtcaaact cattgaagat aatgcagcac gtctaggtgt
    29461 ctcttctgct gtctacgctc aaaagatgga tgctagagaa gtcagtaaac agttcgaagc
    29521 agagcagttt gatcggattt tagtagacgc gccttgttca ggtcttgggc tgcttcggcg
    29581 aaaaccagat attcgttata aaaaacagcc ggatgaatta gcgaatttac caaaaatcca
    29641 attggaaatt ttagaaagct gcgcaccaac tttaaagtct tccggtattc tgacttatag
    29701 cacttgtacg atcttaaaag aagaaaacca agacgttgtt tctgctttct tggaaagaca
    29761 ccctgatttt gagaggattg atgttttgac caatcctatg ctacaatcat caatcgaaga
    29821 gaaaatgttg actttgtatc cgcatcagtt ttacacagat ggatttttca tttgttgttt
    29881 gcgcaaaaag taatgaggtg aaaaaatgca gattgaatat caatcagatg taggaagacg
    29941 acgtaacaca aatcaagact atgccagtgt atttaccagc cgttcaggtc tcaagcttgc
    30001 tgtattagca gacgggatgg gtggtcatcg tgccggagac attgccagtc agatggcggt
    30061 aacgaatcta ggcgaagctt gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca
    30121 atggttcatt caatcgattc aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca
    30181 gccggaatat aacggtatgg gaacaacaat cgtagcggcc gctctgtcag atgactggtt
    30241 cacgattgcc aatgttgggg atagcagagc ttatttgatt cgtgaagata aaatcatgca
    30301 attgacggag gatcattctt tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga
    30361 aatggcagtg aaccatccaa ggaaaaatat tttaacacgt tctgtcggca tgcctggaac
    30421 agtcgaagta gatgtttcta ctcatctttg gcaactaaaa gatcgactgc tgttatgttc
    30481 agatggcttg acgaacatgc tgtctgaaga agaaatagga acgatcatca atcaagaagg
    30541 cccattgtct cataaagtaa ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga
    30601 taatatcacc gttcttctta tcgaatataa ggaggacagt gtatgatcga gttgggcaaa
    30661 aaattaaatg gccgttatca aatcactgga aatatcggta gcggtggtat ggcaaatgta
    30721 tttttggcgc acgatttaat cttggatcgt gatgtagcag ttaaagtctt acgatttgac
    30781 tttcaaaatg accagactgc gattcgacga tttcagagag aagctttagc agcaacagaa
    30841 ctcgttcatc caaacattgt tagtgtttac gatgttggtg aagaagacaa catgcagtat
    30901 ctggtgatgg aatacgtcaa aggaatggac ttgaaacgat atatccaaac acattatcct
    30961 gttccttatg aaacagcagt aaatattatg cagcagatcc tttctgctat ttctttagct
    31021 cataaccatc aaatcatcca ccgagatttg aaaccccaaa acgtactgat cgataatgaa
    31081 ggtgttgtaa aaatcacgga tttcgggatt gctatcgctt tatctgaaac atcgatcacg
    31141 cagacgaata cgatgctagg atctgttcat tatctgtccc ctgagcaggc cagaggaagt
    31201 atggcaacga agcagtcaga tatttatgct ttagggatca tcctttatga gatgttgacg
    31261 ggtagcgtac cgtttgatgg tgaatctgct gtaacaattg ctttaaaaca ttttcaagat
    31321 gatcttccat ctataaaagc acttgatcca aatgtgccac aagcactgga aaacgtgatt
    31381 ttacgggcaa cggctaaaga acctgcagac cgttataagt cagccgagga aatgtctgat
    31441 gatctttcta cagtattatc accagctcgt gcaaacgaag aaaaatggca gcctcatgtg
    31501 atggataatg aaacaaaagt tatcacaccc cttacggaag atgcaccaat gcctgattca
    31561 ttcaaatcta tgcctctgcc aaaagacaag ccagaaaacg aaaccttacc agaggaggaa
    31621 gaagttctgc ctgatcctaa aaagaaagga cagaaaaaat ggtggctgat cctattagct
    31681 attcttgctg ttttaggaat cggaacagct gtattctttg cttctggcgg gcgaggagaa
    31741 gttgttgttc ctgatgtttc tgatctttct gaagcttctg ctagagaaac actcgcaaaa
    31801 gcgaaattag aagtagcaac aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt
    31861 aatgtagtga agacagaccc ggcagcggga acgacagtca agcaaaaacg cgaagtcact
    31921 ctgtatatta gtagcggcac gaaaaaaatc aaattagatg attacagtgg taaaagttat
    31981 gaagatgccg aagcaagttt gaaagaatta gggttttctg ctgacttgat tcaaaaagaa
    32041 gaggaattca gttcagaggt agaaagcgga atgatcattt cgcaatctcc atcagaagat
    32101 acagaagtcg atcctaaaac agacacgatt actttcacag tcagcaaagg accagaaaca
    32161 gtgactttga gtagtcatgt aggagaaaac tacgaaactg ttcgtaattg gcttgttaca
    32221 caaggattca atatcaataa tattagtgct acttatgact atagcgacac tgtaccatca
    32281 ggttcgatca tcagtcagga tccagcctcg ggtgaagttg tagcagaaga aacttacatc
    32341 aattttgtcg taagccaagg aacagagcct aaattgaatg atatctccgg ctatacaaaa
    32401 agcgaagcac aaagttattt agctagtgtc ggtgcagaat atatcgggca tgagacttcc
    32461 gaatattcaa acacggtgga taaagataaa gtgattcgta cagtaccagg ggcaggaacg
    32521 acaattacca aaggaatggt tgtgaacgta atttactcta aaggaccaga tccagcgtct
    32581 tctagttctg aaaaagactc ttcagacagt tctagcagct caagtagctc cagtagcaca
    32641 agcaactctt caaaagaaga atcttccagc tccacaagtt catcagctag cacctcagaa
    32701 agttcaaaat aagtgctgaa taaaatatat cagaagggaa tgcgacattg atttgttgca
    32761 ttctcttttt tgtgtttcta tctcttttga tcaattttca agccatatac tttgagaagt
    32821 ttgcatggaa taacacctac tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg
    32881 cacttatcaa aggacaaatc agaaaagcaa tcagcggttt ttactatgta tacgcaaacg
    32941 gagagaccta tcaaacaaga ggaagaggaa atttccggaa tcggaaaatc acgccgcttg
    33001 tcggagatca ggtgatcttt gagagtgaaa atcaaacaga cggctatctt ctggaaatct
    33061 tgccgagaaa aaatcaatta gttcgcccac cagtcacaaa tgttgatctt ggaatcattg
    33121 tcacaagtat cgtagagcct gcattttctt ataatctact tgaccgcttt ctagttacct
    33181 tggaatacga gtcgatcgaa ccgattattt ttttgacaaa gaccgacttg tcagacgacc
    33241 cagaaaaaat cgaagagatc aagcaaacct atactaaaat aggctatccg gtgattgtac
    33301 ccaatcatac gggtgatacg acagaactga tccgttactt cccagaacgg ctatcggtct
    33361 ttatgggtca gtctggagca ggaaaatcga cgctactgaa tcaaatatca ccggaactga
    33421 atttagcaac aggagagatt tctgatgcac ttggaagagg acggcataca acacgtcatg
    33481 tggaactgat tcctatctat ggcgggttag tagcagatac accaggattt agctcgattg
    33541 actttttgac aattgaagca ggagaactgc cgagacaatt tcctgaattt gtagaagctt
    33601 ctgtccattg caaatttaga gagtgcatgc atgctaaaga acctggctgc gaagtaaaac
    33661 atagagtaga aacaggtggt atcgcacaga cacgttacga caattatttg caattcttac
    33721 aagaaatcga aaaaagaaaa ccaatgtatg caaaaaaaga aggtaaacga aaataggagg
    33781 aaacaagaat gaagatagca ccatccattt tgagtgcaga ttttgccaat ttacaaagag
    33841 acattgagtt agtggaaaaa ggaggcgcag attacatcca cgtagatgta atggatggcc
    33901 gatttgtccc aaatatcaca ttagggccaa atattgttca ggcaatccgt cctgttacta
    33961 aattaccgtt agacgtccat ctgatgatcg ttgatccaga aaactacatt ccagcatttg
    34021 caaaagctgg agcagatatc attactgtcc acgtcgaagc aactcctcat attcatcggg
    34081 cacttcaaat gacaaaaagc ttaggagtta aagctggagt agttatcaat ccaggaacac
    34141 cagtctccat gatcaaacat gttttgcctt tggctgatca agtactagtc atgactgtca
    34201 atcctgggtt cggcggacaa agctttattg aagaaacagt agaaaaaatt acggaactcg
    34261 ctgatctacg tagagagaac aattggactt atgcaatcga agtagatggc ggaatcgttc
    34321 cagaaacagc aaaaatctgt aaagatgcag gggcggatgt cttcgttgca ggatcatata
    34381 tctatggagc cgaaaatcca gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa
    34441 tgaaaatttt attagctgct ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca
    34501 aatacgatgc ctatgtcggg atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc
    34561 cagtagatct ggcagtgggt gattttgatt cattgtcgaa cgaagagcag aaggaagtat
    34621 ttgcaaaagc caaagagtat acccgttccc ctgcagaaaa agacgataca gatacacagt
    34681 taggtatctt aaaaacattt gaacgttacc cgcaagcgga agtcactttg attggagcta
    34741 cgggcggaag attggatcat cttttagcca atctctggtt aggtatggaa cctcgcttca
    34801 aaccctttgc ccatcagctt attatcagag attgtcaaaa ttatttgtct tattatttgc
    34861 ctggagagca tatagtaaga cagataccag atatgaagta tttagcttat tgctgcttga
    34921 ctcccgtctc gaatcttaca ttaaaaggca gcaagtatgt attggaaaat aaagaagtag
    34981 aatatccgac atcttatgca agtaacgaat ttctttcaga taccgcagaa ttttcttttt
    35041 cagatggtat gattgcagtt attcaaagca aagattgatc cataaacaaa taaaaaaatt
    35101 tgtctttcag gctaaaaatg acagaatcta ttatttgcta aaaaaaagct taagaggagc
    35161 aacttacctt ttgtttcacg gcatttagtg ataaaattga acacaagagt ctaattaatt
    35221 cgaggtgtct ttgtgaagaa gttcaatcct aataaaaata ttattattac cttggttatt
    35281 gttatcatcg tggtgacgat actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag
    35341 cccaatttct tccaatccat cgttaatgac agtgttgcaa tggttgataa aacaatctct
    35401 gccccagtac ggtgggtacg aaatggagta gattctgtcc ataatctttt ttcaacttat
    35461 gccgaaaatg agcggctaaa agaaaaaatc gatagctatg atgacgtttc acagcaaaac
    35521 aagaattacg agagagaaat cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg
    35581 actagttacg aaaaagtaac ggcaaatgtc attactcgtt cgcctgacag ttggcaggac
    35641 atgctgatcg tagacaaggg ttctaaagac ggtatcgaag caaatatggc agtcatgtcg
    35701 caaaaaggat tgattggccg agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta
    35761 ctgtcatcct ctaatgaaag ctccaatcat tttccagtac gggtatcttc ggctaatggc
    35821 gaagcgtttg gtttgcttaa aaactatgat gaaaagctcc atgccttagt ggtgacccaa
    35881 ttaactggtg atacggatat caaagaaggg gatgttgtcc agacatccgg tcttggaggg
    35941 aattctccag ctaacttgcc gatcggtacg gttattaaaa cgaaaccaga tagttatggg
    36001 ctggatcggg aagtttatgt gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg
    36061 attgtccaac ggctagcgga ggattagtcc atgttgaaga aagaaacaat gaagtactat
    36121 ctgccaatcg ttttgttctt tttgatgttg atagatggtc atttaacaag aatgctaggg
    36181 gagtggtcga aaggcaccta tatgtcaaat gcccactttc tgatattggc attattatgt
    36241 tgcagtatgg cgtttgaaaa acgttattta ctgattacca cgattgttct cggggctatc
    36301 tatgacgctt actatattgg cgttatcggt atctatgcag tagctctccc tttaattgta
    36361 tggttgatgt atgtaatgaa agacgttatc catgtcaaca tctttactga atttttcagt
    36421 atgatcatct ttgtcacggg ttatgaattg tttacgatgg tggtccagtt gatttttaaa
    36481 ttagcagtag taaataacac gtattttatt acaaggtttt taggacctac actgctgttg
    36541 aacatgatta tatttgtatt attcattttt ccctttaaga aattattcag tgatgaataa
    36601 tttctttttt tatttttagg tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct
    36661 agacttaacg cggctttttc atgatcattt tctgaaacga acagttttgt catttttaaa
    36721 agttgttttg aaacattttt gtaacataac tattatatcg ttgacacact gctatggtac
    36781 aatttttata tcgttaagaa agacttaaat atatttagat atttattaaa ttaagccagt
    36841 cggaggaatg aagagtgaaa aagagtttaa tatcagcagt aatggtatgt tcaatgacct
    36901 tgactgccgt agcatcaccg atcgctgccg cggcggacga ttttgattct cagatacaac
    36961 agcaagatca aaagattgca gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg
    37021 atgccttaga aagccaagtt tctgaaatca acacacaagc acaagaccta ttagcaaaac
    37081 aagatacatt gcgtcaagaa tctgcacaat tagtaaaaga cattgcagat ttacaagaac
    37141 gtattgaaaa acgtgaagat acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca
    37201 caagctcaaa ctacatcgat gctgtattga atgctgattc attagcagat gctattggac
    37261 gcgttcaagc aatgacaacg atggtaaaag caaacaacga cttgatggaa caacaaaaac
    37321 aagataaaaa agctgtagaa gacaaaaaag ctgaaaacga tgctaaacta aaagaattag
    37381 ctgaaaacca agctgctttg gaatctcaaa aaggcgatct tttatctaaa caagctgatt
    37441 tgaacgtact gaaaacttca ttagctgctg aacaagcaac tgctgaagac aagaaagctg
    37501 acttgaaccg tcaaaaagca gaagctgaag cagagcaagc tcgtattcgt gaacaacaac
    37561 gtctagcaga acaagctcgt caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg
    37621 aacaagcaga ggctgaagca caagctactc aagcttcatc tactgcacaa agctcagcaa
    37681 cagaagaatc atcagctaca caatcttcaa tgactgaaga atcatcatca gctacacaat
    37741 cttcagcaac agaagaatca acaacacctg aatcttcaac agaagaatca acagcgcctg
    37801 aatcttcagc aacagaagaa tcaacaacag cgcctgaatc ttcagcaaca gaagaatcaa
    37861 caacagtgcc tgaatcttca gcaacagaag aatcaacaac agtgcctgaa tcttcaacaa
    37921 cagaagaatc aacaacacct gcgcctacaa caccatcaac agatcaaagt gttgatacag
    37981 gaaacggcac aggaagtagt actccggctc caacgccaac accaacacct gaacaaccaa
    38041 aacctgtaac acctgctcca gctccttctg gaagtgtgaa tggtgcagca atcgtagcag
    38101 aagcttacaa atatattggt actccatatg tttggggcgg taaagatcca agtggctttg
    38161 actgctcagg gttcacacgc tatgtttaca tgcaagtaac tggtcgtgac atcggtggtt
    38221 ggacagttcc tcaagaatca gctggaacaa aaatttcagt ttctcaagca aaagctggag
    38281 acttgttatt ctgggggtca caaggcggaa cttaccacgt agcgattgct ttaggcggag
    38341 gacaatatat ccatgctcct caaccaggcg aaagcgtaaa agttggttca gttcaatggt
    38401 ttgcccctga ctttgctgtc agcatgtaag aatga
//
LOCUS       DACCXM010000011        36879 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCXM010000011 DACCXM010000000
VERSION     DACCXM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.011"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            152..523
                     /locus_tag="IVY61_000498"
     CDS             152..523
                     /locus_tag="IVY61_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7969637.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(797..1489)
                     /locus_tag="IVY61_000499"
     CDS             complement(797..1489)
                     /locus_tag="IVY61_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7969638.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1492..2277)
                     /locus_tag="IVY61_000500"
     CDS             complement(1492..2277)
                     /locus_tag="IVY61_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7969639.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2514..3914
                     /gene="lacG"
                     /locus_tag="IVY61_000501"
     CDS             2514..3914
                     /gene="lacG"
                     /locus_tag="IVY61_000501"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7969640.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3939..4256
                     /locus_tag="IVY61_000502"
     CDS             3939..4256
                     /locus_tag="IVY61_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7969641.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4269..5942
                     /locus_tag="IVY61_000503"
     CDS             4269..5942
                     /locus_tag="IVY61_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7969642.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5956..7587
                     /locus_tag="IVY61_000504"
     CDS             5956..7587
                     /locus_tag="IVY61_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP7969643.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8078..9643)
                     /gene="guaA"
                     /locus_tag="IVY61_000505"
     CDS             complement(8078..9643)
                     /gene="guaA"
                     /locus_tag="IVY61_000505"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7969644.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9702..10625)
                     /locus_tag="IVY61_000506"
     CDS             complement(9702..10625)
                     /locus_tag="IVY61_000506"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP7969645.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10821..11426
                     /locus_tag="IVY61_000507"
     CDS             10821..11426
                     /locus_tag="IVY61_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7969646.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11734..12750
                     /locus_tag="IVY61_000508"
     CDS             11734..12750
                     /locus_tag="IVY61_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7969647.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12891..14192
                     /locus_tag="IVY61_000509"
     CDS             12891..14192
                     /locus_tag="IVY61_000509"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP7969648.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14238..14900
                     /gene="deoC"
                     /locus_tag="IVY61_000510"
     CDS             14238..14900
                     /gene="deoC"
                     /locus_tag="IVY61_000510"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP7969649.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14921..15319
                     /locus_tag="IVY61_000511"
     CDS             14921..15319
                     /locus_tag="IVY61_000511"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP7969650.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15385..16467
                     /locus_tag="IVY61_000512"
     CDS             15385..16467
                     /locus_tag="IVY61_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7969651.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16679..18241
                     /locus_tag="IVY61_000513"
     CDS             16679..18241
                     /locus_tag="IVY61_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969652.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18234..19367
                     /locus_tag="IVY61_000514"
     CDS             18234..19367
                     /locus_tag="IVY61_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7969653.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19367..20323
                     /locus_tag="IVY61_000515"
     CDS             19367..20323
                     /locus_tag="IVY61_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7969654.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20422..21588
                     /gene="deoB"
                     /locus_tag="IVY61_000516"
     CDS             20422..21588
                     /gene="deoB"
                     /locus_tag="IVY61_000516"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP7969655.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21612..22430
                     /locus_tag="IVY61_000517"
     CDS             21612..22430
                     /locus_tag="IVY61_000517"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7969656.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22447..23154
                     /gene="deoD"
                     /locus_tag="IVY61_000518"
     CDS             22447..23154
                     /gene="deoD"
                     /locus_tag="IVY61_000518"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7969657.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23452..24138)
                     /gene="gpmA"
                     /locus_tag="IVY61_000519"
     CDS             complement(23452..24138)
                     /gene="gpmA"
                     /locus_tag="IVY61_000519"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7969658.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24310..24891)
                     /locus_tag="IVY61_000520"
     CDS             complement(24310..24891)
                     /locus_tag="IVY61_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7969659.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25024..25707)
                     /gene="rpiA"
                     /locus_tag="IVY61_000521"
     CDS             complement(25024..25707)
                     /gene="rpiA"
                     /locus_tag="IVY61_000521"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP7969660.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26026..26439
                     /gene="rpsL"
                     /locus_tag="IVY61_000522"
     CDS             26026..26439
                     /gene="rpsL"
                     /locus_tag="IVY61_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP7969661.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26523..26993
                     /gene="rpsG"
                     /locus_tag="IVY61_000523"
     CDS             26523..26993
                     /gene="rpsG"
                     /locus_tag="IVY61_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP7969662.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27087..29174
                     /gene="fusA"
                     /locus_tag="IVY61_000524"
     CDS             27087..29174
                     /gene="fusA"
                     /locus_tag="IVY61_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7969663.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29346..30533
                     /gene="tuf"
                     /locus_tag="IVY61_000525"
     CDS             29346..30533
                     /gene="tuf"
                     /locus_tag="IVY61_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7969664.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            complement(30712..32013)
                     /locus_tag="IVY61_000526"
     CDS             complement(30712..32013)
                     /locus_tag="IVY61_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7969665.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            32292..33098
                     /locus_tag="IVY61_000527"
     CDS             32292..33098
                     /locus_tag="IVY61_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP7969666.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            33116..33943
                     /gene="vanW"
                     /locus_tag="IVY61_000528"
     CDS             33116..33943
                     /gene="vanW"
                     /locus_tag="IVY61_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7969667.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVFADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            33940..34911
                     /locus_tag="IVY61_000529"
     CDS             33940..34911
                     /locus_tag="IVY61_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7969668.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            34904..35932
                     /gene="vanB"
                     /locus_tag="IVY61_000530"
     CDS             34904..35932
                     /gene="vanB"
                     /locus_tag="IVY61_000530"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7969669.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            35938..36546
                     /locus_tag="IVY61_000531"
     CDS             35938..36546
                     /locus_tag="IVY61_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7969670.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT        11154 a         6918 c         8279 g        10528 t
ORIGIN      
        1 atattataga atcaacactc agagaacggg ttggttttta atagatttaa taagtactga
       61 taatgaaatt tctcctggtt gggaaaacga agttggttac cctgtagaag aattttttca
      121 agattacttt gcggagtatg ccgataggat aatgagagat tacgtaacag ataaaactca
      181 agaagtcatc aatcaatccc agtcattaaa tgaattatct gataaggcga aagaagaagc
      241 agttgccatt atttcttcag ggattagtga ctatattgat atgagtggtg aaatcaatta
      301 tggcgataaa gacaaaattg tacgcatgat aaaagctttt atcaaaactt ttgatgtgaa
      361 ggcagatctt aagtttaatg atacttacct agtaggaaaa ttaattaata tccttgggac
      421 agagaaagat acggaaacgt ttatttcctc tttgtctatg ttgtttacag ataagcattt
      481 ttcaactcag cttggtggag aggaactagt ggaaatcttt taaccattac gcccagcatt
      541 aataaaagta tatcaaaaaa gtactcctga tgatttttaa gattggtttg agaaataatg
      601 tgaaaacact atttttaatg ttaattatag atagagatga acgcgtcata tggttaaaag
      661 aatgaaaaag tcaaagatac actgctatat ttcactaagg atatagcagt gtatctttca
      721 tgtcgtttca ctggcagttt tttataaaag gaggtgtgca tttcagttgc ttttctttgg
      781 tagttagttg aatcgtttaa gaattaacgg atgtggcaga caggtgctct gtccaaaaac
      841 cagtaaaatc tttgatggtt ccatctttat ttagctgtgc ctgttcaaat actgttttgt
      901 ttttaccgaa tggatcaatt gcaataattg ttccgatccc cgcacgtttg gcagcaagta
      961 atcctgagta agcatcttcg ataacaagac aatcagccgg agctaagctc agtttgtctg
     1021 ccgctttaag aaaaatatcc ggttgcggtt tcccagggaa agagccgtcg tcatagacaa
     1081 tcttgtccca gtcaaaccag cgggctaaat cgaaaatatc aaaataaaaa gcaacattct
     1141 ctttgacagt agccgtggca atcgttaatg gaatctgttg gtcagtcagc tggtcaagag
     1201 tcgcttcaag cccttttgtc aactgaagct cgttttcatt ttgcaagcaa agcatacgat
     1261 aatagctttc tttttcttct gacaatacgg cgatctcaga ggcggtcaaa tgctctgaaa
     1321 tgaaatgttt caaaatctcc gtattcgtcc gaccatgaat attttttaaa atatcctcgt
     1381 ctgataaacc atcttttgta tgatcatgta tcatatgcag ccaagcagct tcatgcagat
     1441 gagagtccaa aaacattgtt ccgttgaaat caaatataat ccctttcata gttacgctcc
     1501 tatagtggta ttttcggttg aatcagcaat ttgcagatcg acatattgtg catagtgctc
     1561 ttgaacctcc cgcgagatct ggcgatccgt aatgagcaga tcgaaatcat gcaaattata
     1621 aaacacataa aaatcatctc gattgaattt tttattatca acgagtagat atttcttacg
     1681 ggaattattt agagcgatct gctgtgcttc tccttcttcg gtactgtaag tagagatcgc
     1741 ttcgttgtga acgccatttg aactgataaa tgcttttgta aactttagtc ttcttaaatt
     1801 gtcgttggtg atcggtccga caaatgcgcc ggtatttttc cggaaatcac cgccaatcag
     1861 tagaatttct ggtccatctg cttgagcaag aatatcaaaa actggataat tatttgtaat
     1921 gatccgaatt ttcttgttca gtaaatgcgt cgccaatagt tcgattgttg tacctggtcc
     1981 taagaaaatc gtttctccct cgtgaatcaa ggaggctgct gtggcagcaa tttctttttt
     2041 ttcttgaatc tgcagagtgg ctttttcagt gtgagaaagt tcttgatcta aagtgtagga
     2101 aagactttga gcccctccat ggacacgcag cagcttgccc gatttttcta attcatctaa
     2161 atctctgcga acggtcatat cagaaacatc taattgattg atgatttcat taacggtaag
     2221 gattcctttt ttattgacca tttctacgat agtaagcaat ctttcttttt tcaacattgt
     2281 cttcacctct aatttaagga taatctatca tataaacgga caacagtcaa tctaattcga
     2341 acaaaagtaa acatttaggg tggtttttat gtttgttaca atagatttta ttgctgtttt
     2401 ctggttttta tgattatttg tgtttgtttt tgttgacaaa tgttttggat gcgtttacaa
     2461 tgcaatcagt tagaaagagc gaaaactgat tatagatttg aggagagatg tacatgctta
     2521 aattaccaga agattttatt tttggcggag ccacagcagc ctatcaggtt gaaggagcca
     2581 ctaaggaagg tggcaaaggc gctgttgctt gggatgactt tttagaggaa caagggcgat
     2641 tcagtcccga tccggcaagt gatttttatc atcaatatgc aaaggacatt gaattgtgtg
     2701 aacgttttgg tgtaaatggt ctaagattat ccattgcttg gagccgcatt tttcctgatg
     2761 gagctgggaa accaaatcca gaaggaatcg ctttttatca tcgtgtgttc gaagaatgta
     2821 agaaaagaaa cgtaacgccg tttgtgacgt tgcatcattt tgatacccct aaacgtttgt
     2881 ttgaccatgg agattttttg aatcgagaaa caatagaagc atttgtttcc tatgccattt
     2941 tttgttttca tgaattcaaa gaagtcaagg tgtggagtac ctttaatgaa atctatccag
     3001 ttgcaaccaa tcaatatttg ttaggtgttt tcccaccagg aatcaaatat gattttacaa
     3061 agatcgtcgc ctgtcttcat aatatgatgg ttgctcatgc ccgtgtagtg aactatttca
     3121 aagaaaacga acttccgggt gaaattggtg tagttcactc attagagacg aaatatgcgg
     3181 caacagacgc gccggaagat aagcatgctg cctttttaga cgatgcattg tcgatccgtt
     3241 tcttattaga tgctacctat ttaggctatt attcaactga aaccttgact gctcttgatg
     3301 agatatgtga agcaaatcag gccagctatc attttcctga agaagatttc gttgaattga
     3361 aaaaagcatc cacgcgtaat gactacttag ggatcaatca ctaccaatgc cattttgtca
     3421 aagcttacga tggagaaaat gcaatccatc ataacggtac gggtgaaaaa gggacctctg
     3481 tttataaagt gaagggaatc ggagaacgta tttataaaga aggaattcct cgaactgatt
     3541 gggactggct gatttatcca gaaggtctat atgacttgtt gctgcgaatt aaaagcgatt
     3601 acccacatta caacaaaatc tatatcactg agaacgggat ggggtataaa gatcagtttg
     3661 aagatggcat tattatggat cagccgagaa tcgactattt gagagtctat ttggaatcct
     3721 taagcaaggc gataacagcc ggggtaaatg tgaaggggta ttttttgtgg tcgcttatgg
     3781 atcttttctc atggacaaat gggtataaca aaaggtatgg cttgttttat gttgattttg
     3841 aaacccagaa gcgttaccca aaagaatcag cttactggta caagttggtc agtgaaacca
     3901 aaacgattat ttaataagtg ataatggagg aagaaaagat gaataaagag gaattacaaa
     3961 tgcttggttt tgagatcgtt gcatattcag gcgacgctcg cagtacatta ttgaaattat
     4021 tgaaagaagt aagacaagga aactttgaac atgttgattc ggcgttgaaa gaagccgacg
     4081 aaaatttaac ccttgcacat aattcgcaaa caaaaatttt agcggaagaa gcatctggga
     4141 aagagatgga gatgggattc atttttatcc atgggcagga tcatttaatg accactttat
     4201 tgctgagaga tctgattcaa gactttattg tactgtacag acagaatcaa ggataggaga
     4261 agaacgctat ggatggaatc attaaacaaa tcgaaaaagg gaaaccattt tttgaaaaaa
     4321 tctcacgtaa tagttattta ggagccgttc gtgacggatt tttaacagcg atgccagcta
     4381 ttttattttc aagcattttc attttgattg ctgcggttcc tgagattttt ggttaccaat
     4441 ggcctgaatc tgtctccaca tggctatgga aagtctataa ttattcgatg ggtgttgtag
     4501 ggttactcgt ttcagcaacc actgctcgct gcttggctga ttcgatgaat cgtcgcttac
     4561 cgaaaaacaa agtaatcaac accacctctg tgatgctggc ctctgtggta agtttcctgt
     4621 tgttaagcgt ctctccgatt gaaggtggat tttcaacgga atatatgggg acaaagggac
     4681 tactggcttc ctttgtcgct gcatttttaa cagttaattt atataagttt tgtgtgctga
     4741 aagatattac cattaaaatg cctaaagaag ttcctggaac gatttcgcaa accttccgag
     4801 atatttttcc tttctctttt tcagtatttg cggccgtttt aattgattta ggcattcgac
     4861 aaatcttcca aatctcattt gcagaagcaa tcattacggt gctgcagccg ctatttacag
     4921 cagctgacgg gtacttagga atcgctatta tctggggagc gatggcgctg ttttggtttg
     4981 tcggtgttca tggaccgtcg atcgtggagc cggcaattgc ggcaattatc tatgccaatg
     5041 tggaaaccaa tctgcaattg ttcaattccg gtgaacaagc ttctaaagta ctgacggtgg
     5101 gtttagggaa ttttgtagga actatgggag gaactggggc aacattggtt gttcctttta
     5161 tcttcctact ctttgctaag tctaagcagt tgaaggcagt tgggaaagct tcctttattc
     5221 cagtaagttt cgctgtcaac gaaccattgc tatttgcggc acccatgatt ttgaaccctt
     5281 atttttttat tccatttttg ttaacacctg ttgtaaacgt ttgcctgttc aagttttttg
     5341 tcgatgtatt gggcatgaac agctttatgt acgttttgcc ttgggcaact ccagcaccga
     5401 tcggtttggt tctaggaacg ggcatgggta tcttagcttt tgttctagca gcggttctaa
     5461 tcgtcgttga cttcctgatc tatctaccat tttgcaaagc atacgatgct gtcttagtag
     5521 agaaagaagc acaaaaagca gaggtgatcg caaccgagga aggtgcttct gccgatgtac
     5581 gtgaggaagt acctgtggca gcgagtcaag ttatcagcga tgcaaataaa gaaatcaatg
     5641 tgttggtact ttgtgctggc gcagggacaa gtgggatgtt agctaatgct ttaaaaaaag
     5701 gtgcatctga atcgaaagca ccgatcagtg caatggcggg tgcttatggt tctcattacg
     5761 aaatgctgca ggattttgat atggtggtct tagcaccgca agtcaattct tactatgatg
     5821 atatcaaaga agacaccgat cgcttaggaa tcaagctggt tgcgacaaaa ggagcagaat
     5881 atatcggatt aacgagagat cctgaaaaag ctgttcagtt tattctgaac caatttcaat
     5941 aaaggaggag ccataatgaa aatgggaatt tggttgcgta aagctgtttt atttgcagct
     6001 gtgttagggg ctttgggggc tgtttcaaaa actgttgaag catctgccag cgaatttatt
     6061 cgcggagcag atatttcgat cctgtcagat atggaaaaat ctggtgcaaa atattatgaa
     6121 gacgatattt caaaagatgc cttagagatt ttaaaaaata atggtgtcaa ctatgttcga
     6181 ctgcgtttgt ggcaggatcc ttataatagt aatggagaaa gttatggagc agggacaaat
     6241 gatctggaaa cgacgattga ccttgcaaaa cgagcaaaaa atctgggact taaggtgctg
     6301 ctggattttc actacagtga tttttgggtt gatcctggga aacagaatct tcctaaagca
     6361 tggcaaggtc tgacctttga agaaatgaat acggcattgt acgactatac aaagaacgtt
     6421 ctgtcggaaa tgaagcagct ggacgtttat cctgatatgg ttcagatcgg caatgaactg
     6481 aatagcggaa tgctctggcc ttatggcaag tcttggggag aaggcggggg agaatttgat
     6541 cgattggctg cttttttgaa gtctggtatc caagctgttc gagacactca acctaaaaca
     6601 actccagtaa tgctccactt agctgatggt ggcgatacag gagcatttac ttggtggttt
     6661 gatgaaatca cgagtcgagg tgtgtcattt gatttaattg gagtgtcgta ttatccgtat
     6721 tggcacggtt caatgaatga acttcaaacc aatatgaatg cgatcagcca acggtatggt
     6781 aaagaagtga tcgtagttga aacagcttat gggcatacaa cagcgaatgc cgatactatg
     6841 ccaaatgctt ttggtgaagc cgaagcagct gcggggggat atgaaccaac accgacagga
     6901 caagcagagt atttgctgga tcttgccgat cggattcagg cagtaccgaa taatcgcggg
     6961 gcgggcttct tctattggga acctctttgg tacaacggaa atgtgagttg ggcaacacaa
     7021 gcaggtatga attatctcag tgttcaatcc actatgggaa acgaatggga caaccaagcg
     7081 atgtttgatt ttgctggaaa cgccttgcca agcttgcgtg cgtttaaaca agcaggggcg
     7141 caaataaatc tagttaaaaa tgcaagtttc gaaagcgatg gaccaacgac cagtccttcc
     7201 aattggcaaa cctggtttca acaaggaacc gctaagggta cgatcaaaac ggaagcgggc
     7261 actttttttg gggactacaa actaactttt tggcaggatc aagcatacga ggcatcagct
     7321 tatcaaaatg ttagtggcat aagcaaagga acttacacat tgagtgccta tgtaatgtct
     7381 gggggagagt ttgatactaa tcagttatat atcaaaaatt atggtggagc ggaactaaat
     7441 actgcggttg ttaaaacaag tgaatggaca aaaattacaa tcaaagacat tccaattaca
     7501 acaggtgttt gtgaaattgg ggtttatagc aaggcttcag gaaatgcttg gacgaacgtt
     7561 gattttgttt cattggtgaa acaatagact gcggatcgtt tcgattcaag atcggaggtt
     7621 cccttcagcc acatcgttag cgtagaaggc aggaatcgaa cctgcacctc gcctttgcca
     7681 aaaataacca gaaacgattt ccagtcattt ttaccaaaac gagtaaagca ccagacttca
     7741 tctacacttt gtattatgct aaagagagtt caacgatttc gtaagaagca atgaaaaaat
     7801 tgcaaaaagt tgtgcagata acataaagtt tgtttaagaa agggtaacct tagaattccg
     7861 tcgtgaatat gatttgagca cttagatgga tagaaaaaag ttctcggaaa acagttcgtt
     7921 tttcgagaac ttctttttat gataagaatg aaacaaatta acttatatta tagtctcttt
     7981 tggtagggaa ccatcttatt tttaatgaaa atcaagcatt ttagctattg aagaatgttg
     8041 atttataggg attccccctc catattatgt tggctcttta ttcccactcc actgttgctg
     8101 gtggtttaga agtgatgtca tacacgatac ggttgacgtg ggctacttcg tttacgatac
     8161 gtacagagat tttttgcaag acttcccatg ggatacgggc aaagtcagcg gtcattccgt
     8221 cgattgaagt tacagcacgg ataccgactg tgtagtcgta agtacgtccg tcacccataa
     8281 caccgacaga gcggatccct ggaagaacag tgaaatattg ccagatatca cggtcaagtc
     8341 cagcagctgc gatttcttca cgcaagatag catcagattc gcgtacgatt tctagcttgt
     8401 cttcagtgat ttcgccaaga acacgaatac caagaccagg accagggaat ggctgacgcc
     8461 aaacgattga atcaggcatg cctaactgag tccctaattc acggacttcg tctttgaata
     8521 atgtgtttaa tggttcgatc aattcaaatt gcatatcttc tggcagtcca cctacattat
     8581 ggtgagattt gatcgtctgt gctgtttctg taccagattc gataacgtca gtatacaacg
     8641 ttccttgtgc taggaaagag acgccttctt caccagcaag ttttgttgct tcgtcatcaa
     8701 aaacatatac gaattcattc ccaatgattt tacgtttttg ttctgggtca gatacacctt
     8761 tcaacttgtc taagaagcga tctcttgcat tgactttgat gatgttcaag ccgaattttc
     8821 cgcctagact ttccattact tgttccgctt cgcctttgcg tagcagaccg tggtcaacaa
     8881 agatagatgt caattggtca ccgatcgctt tttgtaaaag aaccccaacg acactagaat
     8941 caacgccacc tgaaagacca agcaagactt ttttgtcacc aacttgttca cggatttttt
     9001 ctacttgcat atcgataaag ttatccattg tccagttacc ttcacagtga caaacatcaa
     9061 aggcaaagtg gcgcagcaat tcgattccgt attcggaatg acgtacttct gcatggaatt
     9121 ggataccata aaaattacgt gttgtgtctt ggatagaagc aatcggacaa tctttgcttg
     9181 ttccgactgt ttcaaagcct tcaggaactt tagttaccaa gtcaccatgg ctcatccata
     9241 ctgtttgttg ttttggtgtt tctttaaaca agacagcttg atcgcttgtt acttctaaca
     9301 aggctttccc atattcgcgg tttttagcag gctctacgga accgccaagt ttgtacgtga
     9361 tcaattgcat accatagcaa atccctaata caggaatacc taaattgaag atctcaggat
     9421 cgatcccaaa tgcattctca tcatatacac tgtttggtcc gccagaaaga atgattccct
     9481 ttggattcat cgcttttact tcttctgcag taatgcggtg gctcaataat tcggaaaaga
     9541 ctccaagatc acgaatacgt cttgtgatca attggttata ctgactaccg tagtctaata
     9601 cgataatctt ttcaacgttc gtcatatcgg caacgtttgt caaatttatt acccctatcc
     9661 ctttaaattt tatctcaaaa tagtcaaact attttaaaat gctaatattt tcgcatgaaa
     9721 atcgaatcga tgatatgatt ttcagtttta tggagaatta tatcggctct tcctcgtgta
     9781 ggcaagatat attcctgtaa atttttcaaa ttcacatttt tccatacatc ttttgccatt
     9841 ttaaaggctt cttcacgatc tccgatagca aaacgataat aataattctc tggattttgg
     9901 aaagccgtat ccagtaacgc accaaaacga tccaaatacc atttttcgat cagcacggga
     9961 tcggcatcga catagacaga aaaatcaaaa aagtcgctga cgtagatttg ctgattcgca
    10021 ggtaattgaa gcgtattgat cccctcaacg atcaagatat caggctgctg gatcaattcg
    10081 tacttgtctg gaatgatatc atacacatca tgtgagtaaa caggtacttt tatctcttcc
    10141 tgaccagatt ttacacggtt caagaaatcg atcagcatct ccatatcata actttcagga
    10201 aatcctttac ggtccatgat tccacgctct tttaatatac gatttggata aagaaatccg
    10261 tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac gagacaaaat cgtctgcagc
    10321 agtctggctg ttgtactttt tccaacggct acacttccgg cgatcccgat aataaaaggc
    10381 ggtacttcta cgtatttatg aagaaataaa cctttactca agaacaaaga ttcaaattct
    10441 ttcatataaa gataaatcag atgagcaaga ggtacataga tatctcgaac atcctgcatg
    10501 gaaatgcggt cattcaaact tttgatatta tctagttcat cttgtgtcaa aggtgcttgc
    10561 ccattacgat aaaattctcg ccattcttct cttgccacac ggtagtagtt cattggatca
    10621 ttcatattag cctcctgcgt tctcccatca atggaactat tttaacacaa aataaacgca
    10681 aaccccacca cttaagagag attgccgatc tttttgtata atctttataa tagaaagaaa
    10741 cggagtctta ttcttgactt gcgcacggat acataaaagg atagactgtt gtcatgaaag
    10801 aaatgagaag gatggaagac atgacaaatc attactattc tgaacgccca gatacggcgc
    10861 acgattttga acaatgggct tttgagttaa aaggcagaac ctttcaattt gtaacagatt
    10921 ctggtgtatt ttcacgtgaa acagtggatt atggttcacg tgtcctgatt gatgctttcg
    10981 attgggaaga gttacctgat gaaggaaaga tcttagatgt gggatgcggg tatggaccga
    11041 ttgggttagc tttggcattt gcgacacagc ggtttgttga aatggtcgac attaatgaac
    11101 gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca aatcgaacat gttgatatcc
    11161 atcaatcgaa tatctatgaa gcagttcatg aagaaaccta tgcagctatc gtaagcaatc
    11221 cgccgatccg tgctggaaaa aaagttgtac atgaaatttt gacaggcgca tatcctcggt
    11281 tgaagaaggg cggtacactg acaattgtga tccagaagaa acaaggagcg ccgagtgcgc
    11341 aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt cacaaaagat aaaggatatt
    11401 atatcatcaa aagtgtgaaa gaataactag ctgaaaaata gtggagcgac gaaagagtgg
    11461 aactgggcgt gcatgtacgg gtacgtatca gttctttttt tgttgagaag tttttaaaaa
    11521 caaagaacta ttttttcttc aaaaagaaat agtaggaaca gagtcgttgt tttttacgat
    11581 aaaataaata agatacagaa cttgtataga aggctgccaa gtcggtagat aaaggaggtt
    11641 tttatcaaaa aacatacttt ccttttttct ctattctttt cttgggggtt ttggtacact
    11701 gatagggaat ttagatagaa gggagccaat aggatgaagc tgaccatcag agatatcgca
    11761 gaaatggcag gcgtatcagt aacaactgtc tcacagattt tgaataacaa aggaagcaga
    11821 tttagtgata agacaagaaa aaaagtgttg tcgatcgtga atgaacatca ttataagccc
    11881 gattatttcg cttcaaatgt gatcaatcga cattcaaaga ccatcggaat gatcgtccct
    11941 gatgtcactg atttcttctt ctccaaactg atcgaaggtg tagagagcta tttgaatcct
    12001 ttgggctatg tgatcatgtt atgcaattct cgtcatagcc aagaaaatga gatcaagtat
    12061 ttacaagagc tttcccaccg ctcagtggac ggaatcttgc tggccacacc aaatatttta
    12121 cctgagaaat atgcattaga cagcggtttt tatcaaaaaa tgccaatcat tttaatcgat
    12181 cgcggattga atcgtcggga taacggacgg cttatcgtaa aggaatatga gggcgcctat
    12241 caagctgttt cgctgtttat tgaaaatggc catacgaaga tcggcatgct gaaggaaacc
    12301 actggatact atcaattaga agaacgtttc aacggatatc gtcatgcgct gaaagatcat
    12361 ggcctgcctt ttaatgggaa atttgttgaa catggagagt tgactgtcca aggtggatat
    12421 actgcatcaa aaaaattact caaacataaa gatatcacgg ctattttttg cgggaatgat
    12481 gccatggcga tcggatgcta tcaggcgatc gatgaattgg gcaaaaaaat cccagaagat
    12541 atctcagtca taggattcga tggattaaag ctgtcagaat acatgattcc gcaattgacg
    12601 acagtgcagc agccaagttt tgatattgga ttttatgctg cacgtttcct gatcgatacg
    12661 atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg aaacaaaatt aattattcgt
    12721 gagagcgtaa aagtattgac aaagggctaa ctcggcggta tattgcttat gtaagcattt
    12781 acaaaggcta gttagtgctt tttttatttt tagtaaacca gtttactaaa gtggtttacc
    12841 tttcctaaaa ataatgtata ataccgaatg aagacaagga ggatcaggca atgcgaatgg
    12901 tggatctgat tgaaaaaaag agggatggaa acgagctgtc gaaagaggaa atcgagtaca
    12961 tcgtcacgaa ctatacgaat ggaaagattc cagattatca agtcagtgca ttattgatgg
    13021 caatttttta tcaagacatg acaaatgaag aaatcacgaa cttaacattg gcgattgcta
    13081 attctggtga tgtcatcgat ttgagttctc ttgaaggaat aaaggtagat aagcattcga
    13141 caggtggcgt tggtgacacg accacattga ttttagcccc cctagtagct agtgtcggag
    13201 tgacagtggc taaaatgtct ggacgcggtc taggttacac aggtggtaca ctagacaagc
    13261 tagaagcaat tcccggattt cagatcgagc tttcggatga agaatttgta cgcattgtta
    13321 atgaaagtaa agtagcggta attggtcaat caggaaactt ggcaccggcg gacaaaaaat
    13381 tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct tccgctgatt gccagttcga
    13441 ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt tttggatgtg accactggag
    13501 acggtgcttt catgaagaac atcgaagatg caagacgtct ggcaaagacc atgacgagca
    13561 ttgggaaact ggctaaccga gaaacagtgg cggttatctc agatatgtca gaacctcttg
    13621 gcgaagctat cggaaatagt ctagaagttg tcgaggcaat cgaaacatta caaggaaatg
    13681 gtccagaaga tctagtagaa atgtgctacg ccttaggaag ccaaatggtt gttcttgctg
    13741 gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca agaagctctg gaatcaggta
    13801 aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg gggagaccca gcgatcgtgg
    13861 aacatccaga acgaattttg actgcacgct acacaatgga gcttcctgct aaacaaagcg
    13921 gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc tgcaatgatg ctaggtgctg
    13981 ggcgtaaaac caaagaagaa gatattgatc acgctgttgg attgaaactg cataaaaaaa
    14041 ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat ctatagtaac gacgaagaaa
    14101 tcacttctgt cattgaatta ttatataaaa atattgaaat tggtgaatcg gcaatgaagc
    14161 caacgttgat tcatgacatc atcacagaat aaaggaaaaa agcggacttt accgttttaa
    14221 agaaaagggg aaaagaaatg gaactaaacc gaatgatcga ccatactatt ttgaaagcag
    14281 acgcatctaa agaagctgtg atgcaaatta tcgaagaagc aaaaaaatac catttttatt
    14341 ctgtctgcat caatccagca tgggttgcgc tagcgaaaga acaattacaa ggtgagccag
    14401 tagccgtatg tacagtaatc gggtttcctc taggagcaaa cacacccgag acaaaagcat
    14461 ttgaaacaac agatgccatc aataacggtg cagatgaagt cgatatggtc atcaatatcg
    14521 gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga tatcgaagca gttgttgaag
    14581 cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac atcgttatta gatagagaag
    14641 aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc tgattttgtc aaaacatcaa
    14701 ctggtttctc aacaggcgga gcaaaggtag aggatgtccg tttgatgcgt gaaactgtag
    14761 gaccagaaat gggtgtcaaa gcttccggcg gtatccataa cgaagaagaa gcaatggcaa
    14821 tgatcgaagc tggtgctaca agaatcggaa caagcgcagg agtagcaatc gtatctggtt
    14881 caactggtga aggctattaa ttttttcaaa ggagaagctc atggcagaac tcaaacaaga
    14941 atggatcaac acagctattg aagcgttaga taaagcctat gttccttatt cccattttcc
    15001 ggtaggcgct tgtctcgtaa cggaatcagg caagatttat caaggcatca acattgagaa
    15061 tgcatcattt ggattaacga attgtgccga gcgcacagct tttttcaaag cagtatctga
    15121 aggagaaaaa acatttacgc atctagtcgt agcggggcac acccccgatc ctatctctcc
    15181 ttgtggtgca tgtcgacaag taatggcaga attctgttcc cctgatatgc cggttacgct
    15241 agtaggagac aatggtgtga cgaaggcaac gaccgttcgt gcactgctgc cttatgcatt
    15301 tactgaaaaa gatctataac tcatgttcat tcagtcagct gactgttgaa aaataaaaca
    15361 attattttag gaggctttac agaaatgaaa aaagcaaaat tatttggttt aggtgctgtc
    15421 gcactagcag cagggttatt tttaggtgca tgcggcaaca acggctctac tgattcatcc
    15481 ggtggcaaaa gctcatcaga cacaacaact gcagcattga tcacagacac aggtggtgta
    15541 gacgaccgtt cattcaacca gtcagcttgg gaaggactag aaaaatgggg gaaagatcaa
    15601 ggactttcac gaggaaatga tggcttccaa tatttccaat cttcaaatga atcagattac
    15661 atcccaaata ttgaccaagc attgaatgct ggattcaaaa cgatttttgg tattggttat
    15721 aaactaaaac cagcgatcca agaacaagca acaaacaata caggtacgaa ctttgttatt
    15781 atcgatgatg tcatcgatgg attagataac gttgtatctg caactttcaa agacaacgaa
    15841 gcttcttatc ttgcaggtgt tgcagcagct tatacgactg aaacaaacgt agtcggcttt
    15901 atcggcggtg taaaaggtga agtaatcgat cgttttgatg ctggattcaa agccggtgtt
    15961 gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat tgaatcaata tgctggtgac
    16021 ttctctgctc cagataaagg acgttcaatt gctcaaggta tgtatgcaca aaacgcagat
    16081 atcatcttcc atgcttcagg cggtacaggt aacggtgtat tccaagaagc aaaatcattg
    16141 aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg accgcgacca atcagacgaa
    16201 ggcgaataca cattaaacgg cgaaaagaaa aacttcacat tgacatctac actgaaagca
    16261 gttggaacag tagtagaaga tttggcacaa aaatcagcgg acggtaaatt ccctggcggc
    16321 gaacacactg tttatggatt gaaagaagac ggtgttggtc taacagaagg acaactttct
    16381 gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa tcatttcagg tgacgtaaaa
    16441 gttccagaaa ctcctgaaga aaactaaaac catttctata gccttgttag acacatgttt
    16501 gacaaggcta tggtttgatt agaacaagaa ttgttcattt atccaagctg ccttgtgaca
    16561 gtttggataa gggaacaact ccctgaagta atgtttgttt caaaagaaga acgctcacta
    16621 agcgtgaatc ttagtcaatc gttgccctag atagaaatcg ttaggatgtg aaaaaaaggt
    16681 ggttcaagaa aatattgtca tagaaatgca gaatattacc aaagaatttg gaacattcaa
    16741 ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa attcatgcgt tgcttggaga
    16801 aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc ggattattag agccgacatc
    16861 agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca agtccgacca aagccaacca
    16921 attagggatt gggatggtcc atcagcactt tatgcttgtt gatgccttta ctgtaacaga
    16981 aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg cttgaccata aaaaagcacg
    17041 aaaagagatc caaaaagttt ctgaacagta tggattgtca gtcaacccag atgcttacgt
    17101 tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt ttaaaaacac tttaccgagg
    17161 agcagatgta ctgatttttg atgagccgac agctgtattg acccctcaag aaattgatga
    17221 attaatcgtg atcatgaagg aattagtcaa agaaggcaag tcgatcattt tgattacgca
    17281 taagttagat gaaatcaaag cagtagctga ccgctgtaca gttatccgcc gtggaaaagg
    17341 aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa ttagccgata tgatggttgg
    17401 aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag cctcaagaag tcgttttgtc
    17461 tattgaaaat ctagtggtga aagaaaatcg tggattagaa gccgtgaaaa acttgaactt
    17521 agaggttcgt gctggcgaag tacttggtat cgctggaatc gatggaaacg ggcagtcgga
    17581 gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc ggacatatca agctaaaagg
    17641 ggaagacatc actaataaaa aaccccgaaa aatcactgaa catggtgtag gacacgtgcc
    17701 agaagaccgt cataaatacg ggttagtcct agatatgaca ttatctgaaa atatcgcact
    17761 acaaacctat catcaaaaac cttacagtaa aaacggcatg ctgaattatt cagtaatgaa
    17821 tgaacatgcc agagaattga tcgaagaata tgatgttcga acaacgaatg aacttgttcc
    17881 cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt atcgctcgtg aagtcgaccg
    17941 agatcctgat ctgttgattg ttgcaaatcc aactcgtgga ctggatgtag gtgcgatcga
    18001 atttattcat aaacgtctga tcgaacaaag ggacaaatac aaagcagtgt tactgatcag
    18061 tttcgaatta gaagaaattt taaatgtttc ggatcgtatt gctgttatcc atgaaggaga
    18121 aattgtcggt atcgttgatc cgaaagaaac atctgaaaat gaattaggtc tattgatggc
    18181 gggctatacc ttagaagaag cacgaaaaga gttagaaaag ggggctggcg cacatgaatg
    18241 atcgcagcga aaaattccgt aatttattag tgcctgtact ttcagttgtt ttaggattga
    18301 tcttaggcgc gatattgatg gctgcttttg gttttcagcc catccaaggt tattcttcta
    18361 tgctagatgc agcccttggt tcgcaaagag ggataggtga aacgttaagg caagcaacgc
    18421 cgttgatttt tacagcatta ggtttttctg tagctaactc agctgggttt ttcaatattg
    18481 ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt ctgggtggca cttgcgtttc
    18541 cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat cttaggtgcc ttagctggtg
    18601 cgatggcagc ggctgttcca ggtttacttc gagcattttt tggaacaagt gaagtgatcg
    18661 taacaatcat gatgaactat atcttattat acgtaagcac atttgcttta catgaagtga
    18721 tgcctgaaag ctatcgctca agtttggact cttccaatat gatcagtcaa aatgcctcac
    18781 tgcggttacc atggttaaca caagcttttg gcggctctcg cgtaaactca ggattatttt
    18841 tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa gaaaacgact ttaggatttg
    18901 aaatccgttc tgttggattg aatccttttg cttctgagta tgcaggaatg agtagtaaac
    18961 gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc agggcttggc ggtgtggttg
    19021 aaggattagg aacgtatcaa aacttctttg ttcaaacgac ttcgctatct attggatttg
    19081 acggaatggc tgtttccttg cttggttctg gatcagcaat cggtattctt ttatcagcga
    19141 tcttgttcag tattttgaag atcggtggac ttggcatgca gacaggagca ggtgttccgt
    19201 ttgagatcgt caatgtatcg attgctttga ttatcttctt tgtcgggatc aacttcctga
    19261 ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca agaagagatc gttgcgacaa
    19321 tagaatcgaa accaacgaat caaaaccatg aagggggcga actataatgt cgactatcga
    19381 attaattgcc ttgatcctta cttctacact ggtttattca acaccgctaa ttctaacgtc
    19441 actaggcgga actttttctg aacgaagcgg gatcgtaaac gtagggctag agggaatcat
    19501 ggtcatgggt gcttttagtt ccatcgtttt caacttgacg atgagtccat cacttggttc
    19561 tatgacacca tggatcgcta ttttagtggg tggcgtcgta ggtatcctat tttctctgat
    19621 tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt atcagcggga cagtaattaa
    19681 tctgatggca cctgctttag gtgttttctt gatcaaagca tggtatggta aaggtcaaac
    19741 tgataacatc acgcagaatc tggggtattt ttctttccca ggattagcag atattccggt
    19801 cattggacca attttcttta aaaatacatt cttacctgct tattgtgcta ttttgattgc
    19861 tgtcctagct tgggtgatca tcttcaagac gaaattcggc ttgcgcctcc gttcagttgg
    19921 tgaaaatcct caagctgccg acacattagg tatcaatgta tatgctatgc gttattcagg
    19981 cgtactgatt tctggcttgt taggcggtat gggcggtgct gtttttgctc agtcgatttc
    20041 cggtaacttt tcagctggta cgatcgttgg acaagggttc atctccatgg ctgctatgat
    20101 ctttggtaag tggaatcctt taggggcaat gggtgcctca ttgttctttg gttttgctca
    20161 aagcttgagt atcattggcg cacaattacc agttatttct tcgattccgc cagtattgct
    20221 gcaaattgct ccatatttgc tgacaatcat tgtgctggtt gttttcttag ggaaagcttc
    20281 tggtccaaaa gcaaacggga agaactatat caaatcaaaa taatctttta gcgaataaaa
    20341 agggtgggtt acgggaagtg tttttcgtct cgcccttttt gttttaaagg caataaaaat
    20401 aaaaagaaag aaggaataat catgtttaaa cgcgtacatc tgatcgtcat ggattcagta
    20461 ggaattggag aagcaccaga tgccgataag ttcggtgatg ttggttctga tacattagga
    20521 cacattgcga aagaagcagg attgaacgtt ccacatttag agcagcttgg tttaggaacg
    20581 atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc aaggctatgc gacgaaatta
    20641 gaagaagttt ctgtagggaa agatacgatg acaggccact gggaaatcat gggattgaat
    20701 atcaaaactc catttcgtgt gtttcctaat ggattccctg atgatctgat caaacaaatc
    20761 gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt atagcggaac agctgttatt
    20821 gacgattacg gtgaacacca aatgaaaaca ggcgatttaa ttgtatatac ttctgccgat
    20881 cctgtccttc agatcgcggc acatgaagaa atcattcctt tagaagaatt atatcggatt
    20941 tgccaattcg ttcgtgatat aacaaaagat gatccgtata tgattggccg aatcattgca
    21001 cgtccgtacg tgggtgaacc aggagacttc acacgaacaa gcaatcgcca tgattatgcg
    21061 ttgaatccat tcggacacac agtccttgat tctttgaaag aagcaggaaa ggacgtgatt
    21121 gctgttggaa agatcaacga tattttcaac ggacaaggaa tcactgaatc tgtccgcacg
    21181 aaaagcaata tggatggcgt tgaccagctg ctgaaagtta tgaaaaacga tttttcagga
    21241 atcagcttta ccaatttagt agattttgat gcattatacg gtcatcgtcg ggatgtagtc
    21301 ggctatgcgc atgcgattga agaatttgac ttacgaattc cagaattgct tgaagcgatg
    21361 gaagaagacg acttgctaat gattacagcc gatcacggaa acgatccgac gtttactggt
    21421 accgatcata cgcgagaata tgtaccgtta ttggtttaca gtaaaaaaat gaaagaacaa
    21481 ggggcattgc ctcaaggata ttattcagat atttcagcaa cgattgctga gaactttagt
    21541 gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat taaaataatc tttttaaata
    21601 ggagaaagca aatgaccaag ttaagcgaga tgttacaaga aacgacccag tttatccttg
    21661 ataaaggagt aaaagaaatt gattttggtt tgattctagg ttcaggatta ggtgaattag
    21721 cagatgaaat cgaagatgct gtaatgattt cttatgatga gatcccgttc tttcctgttt
    21781 cgacagtagt tgggcatgca ggaaagctag tttatgggtc tctggcaggc aagaaagtgt
    21841 tagccatgca agggagattc cactactatg aaggtcattc catgcaaaca gtgacctatc
    21901 cagtacgtgt gatggccgga ctaggggccc attcagtcat tgtgactaat gcatgtggtg
    21961 gtgtcaacga atcatttgta ccaggagatt tgatgctgat taccgatcat atcaatttta
    22021 ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat gggccctaga tttccagata
    22081 tgagtcaagc ttatacgcct gactatcaag caacagcaaa agcagcagca gaaaaactag
    22141 gtttggatct taaagaaggc gtatatatgg gctattctgg accaacttat gaaacgcctg
    22201 cggagatccg aatgtctcgg acaataggag ccgatgcagt agggatgtcg actgttccag
    22261 aagtcatcgt agcagctcac agcggattaa aagtgttagg aatctcttgt attaccaact
    22321 tagcagcagg tatgcaagcg aatctgagcc atgaagaagt tgtagaaaca actcagagag
    22381 tcaaacaatc ttttaaagca ttggttaaag aaacattgac attattgtaa taggaggaac
    22441 aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga tcgccgataa gattttactt
    22501 ccgggagatc ctttgcgagc taaatatatt gcggagactt ttttggaaga tccagtttgt
    22561 tacaatcagg tacgcggtat gttaggcttt accggcaaat ataaaggaga acgtatttct
    22621 gttcaaggaa caggaatggg aatgccatca gccaccatct atgcccatga attgatccag
    22681 tcgtatggtg taaagaaatt gattcgagta ggtacatgcg gtgcattgtc aaaagacgtc
    22741 catgttcgtg atttagtact agcacaaggt gcagcgacca gttcttcgat gatcgaaaaa
    22801 aatttccaag cctttcattt tcctccaatc agtgatttca atctattact gaaagcatat
    22861 gagatagcta aagaaaaggg ctatacggtt catgtaggaa atgttctttc ggaagattct
    22921 ttctacaaag atgatttgac agagacgttc caattagctg aacttggcgt tttaggagtg
    22981 gaaatggaag cagcggctct ttattactta ggcgcgaaat accatgtgca gacattaagt
    23041 ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa caacagctgc agaacgtcaa
    23101 tctacgttca atgaaatgat cgaagtagga ttggaaacag cgatagcaga ataagaagga
    23161 aatgaaaaag ctgccctgca ttaagtaata agaacatcca agcaaaaata gctcctcatg
    23221 tttttgactt ggctggactt attattgcaa atgtaagctt ttgaacaccg tttaccaaga
    23281 ggttgtgaat ctgccgttta actctgagtc acaaggaaca atttcaaaaa acagcttttc
    23341 atgtttgatg aatattgtga cttgtgaccg agaagttggc aggagaaaac cgtttatacg
    23401 aaaaaaggga ctgtgacaaa tcgtgtcaca gtcccttttt tatagtgttt tttataggta
    23461 gtatttttta gcaactgtta agtcatcatt taattcataa acaagtggtt gacctgttgg
    23521 aatttcaaga tccatgatgt cttcgtcaga gataccttcg atatgtttag ctagggcgcg
    23581 taatgagtta ccatgtgcag ctactaaaac agttttgttg tctagtaatg ctggagcaat
    23641 ttcatcttgc cagaatggca aagcgcgttc taatgtcact ttcaagtttt ctccaccagg
    23701 aatatcgcgt ttgtctaaca ttgcgtaacg acggtcattt gctgcagaac cttcgtcagt
    23761 ttcttccatt aatggaggaa gagtatcgta agaacgacgc cagatgtgca cttgttcgtc
    23821 gccgtatttt tcagctgttt cttttttgtt caaaccttgt aatttaccat agtgacgttc
    23881 gtttaagcgc catgatttga tttgtggtac ccaaagttga tctgaatatt ctaagatcaa
    23941 gttacatgtt ttgattgcac gagttaaaac agaagtgtaa gcaacatcaa attcgattcc
    24001 agcttctttg attttgcgtc cgccttcttt tgcttcttcg attccttctg gtgctagatt
    24061 tacatcagcc caaccagtaa attggttcaa tgcattccat tcactaagac cgtgacgaga
    24121 aaaaactaat tttggcatgt ttagctctcc ttttaagata ggtttatatt tctgaaattt
    24181 tcagttctgt tttattgtac aattttttgg aagagatttc catcgaaaaa ttagataaga
    24241 ttctatgaga aaacaagtag agaatataaa aaaacgaaca gtgcccacgc aactgttcgt
    24301 ttttaggtat tagatgcata attgctcgat ttctttttca ggtaaattga tatatttata
    24361 acatgttcca actgaaacac cgcattttga agaaataaat tggatcgttt cttttttttc
    24421 ataatagaga cgtcggattt ttttgatcgt ttttgcatcg atttttggtc ttccgccgat
    24481 ttttcctttt ttacgagctt catctaatcc aactaatgta cgttctttga tcaatgcgca
    24541 ctccatgttt gctaggtttt ccattaattg gaaatagatt tttcccattt cgcgcgtatc
    24601 gattccttca tcaagactaa tcaagtgcag cccgcgatct ttgaataatt gcgtgagttc
    24661 tgttaattgc cgcgtagatt tgcctaatcg atgaagttgg caaatgacta gtgcatcgcc
    24721 ttgttgcatg tcagcgatca ctgactccaa atgaacagca gtagaatcat tttgttccac
    24781 ttcatatgtc tcatggaaaa tttcttgaca accaaagtca gataattgat caacctgagt
    24841 tgttaaatca acgtcattta tggttgtgcg tgcataacca ataattttca tggtgattcc
    24901 tttctagcag tgattttcta actggatata acagtaaaaa tgtagcataa agtacgtcaa
    24961 aaagatagtt ttttcgcaaa ttttccgctg tcttttttta ttggatgatc aagcagtttt
    25021 tctttattta cgacggattg cgtcaagaat ctcaacgcct ttttctgagc cgacaactac
    25081 tttagaaacc atgtttagga aaagcccatg ttctactacc ccaacaagtt catctaaata
    25141 ggtagctaag ctttctggct gttcgatgac ttctagatgc agatcaatga tgtaatgacc
    25201 accatctgtg gttaagactt ctccgttttc gtctgtacgc aagacgggtt ggaatctttt
    25261 ttcttcaaaa atatgaagta gctgttggga gccataaggg atcacttcaa ctgggagtgg
    25321 gaacttccca agtttatcga ctaatttaga agaatcaacg atccaaatcg tttcttttga
    25381 gtacgtagcg actattttct cgaaaagcaa agcagcgccg ccgcctttga tcccttgaaa
    25441 atcagcactg atttcatcag caccgtctat tgtcaaatcg actacgggga cttcatcaat
    25501 actctttaac ggtattccta atttttccgc ttgttctttt gtcgcatttg aagtagtaac
    25561 tcctgtgata cgtaaacctt cttcacgcat acggcgacca atctcttcta ccatataata
    25621 ggcggttgaa ccggtcccga gacctacgat catcccgtcc tttacgaatt tcgcggcttc
    25681 aatgccagct aattgtttta aattcatctt tgcgtccacc tttttttagt ttgagcatcg
    25741 tgctttcctc actattttag cgtgatttac ggtaaaaaga taccattctt ttttattcca
    25801 ataaagcttt ttcaaaaaaa attgcatctc ttgttgacat gttttttcat tcgtgatatt
    25861 ctaacaaagg tgctttatct acaggtttct gtgaagaaac gtgctgactg tttatcaaag
    25921 catatttgat aaggatgcag acattgcatt tattttttat cacaaaaaaa gaaccacctg
    25981 gatgtgtgga actagacgca aatcgtggga aggaggaaac aaggaatgcc tacaattaac
    26041 caattagtac gtaaacctcg taaatcaaag gtggaaaaat ctaattcacc tgctttgaac
    26101 aaaggatata acagctttaa gaaaactcaa actaacgtaa actcacctca aaaacgtggg
    26161 gtagcaactc gtgtgggtac aatgacacct aaaaaaccga actcagcttt acgtaaatat
    26221 gcacgtgttc gtttgtcaaa cttaattgaa gttactgctt atatcccagg gattggtcat
    26281 aacttacaag aacacagtgt tgtattatta cgcggtggtc gtgtaaaaga cttaccaggg
    26341 gtacgttacc atatcgttcg cggtgcttta gatactgctg gtgttaacga ccgtaaacaa
    26401 agccgttcta aatacggaac taaaaaacct aaagcttaat tttaattatt aaacaaatga
    26461 aacttgcttc tactatatag aagtaattac tatgaacaat atttcggaag gaggagttac
    26521 ggatgccacg taaaggtcct gttgcaaaac gtgatgtttt accagatcct atttataact
    26581 caaaattagt aactcgcttg atcaaccgtg taatggttga cggaaaacgc gggattgcag
    26641 ctaacattat ctataatgcc tttgacttga tcaaagaatc tacaggtaac gatccattag
    26701 aagtgtttga acaagcaatg aaaaacgttc aacctgttct tgaagttaaa gcacgtcgtg
    26761 ttgggggttc aaactaccaa gtaccagttg aagttcgccc tgaacgccgc gttactctag
    26821 gtcttcgttg ggtagtaaac tacgctcgct tgcgcggtga acacacaatg gaagaacgct
    26881 tagcaaaaga aatcatggat gctgcaaaca acacaggtgc ttctgttaaa aaacgtgaag
    26941 acactcataa aatggctgat gccaaccgtg cgtttgctca ctatcgctgg taagattctc
    27001 tctgtttggt cggcttttat aagtcgacct cagatagtgt ccttattttt agcaactaga
    27061 tagtaaagag aggagaaaca aggagaatga ccagagaatt ttctttacaa aacactcgta
    27121 atatcggtat catggcccac atcgatgctg gtaaaacgac tgctactgaa cgtatcttgt
    27181 actatactgg taaaatccat aaaattggtg aaactcatga aggagcttca caaatggact
    27241 ggatggaaca agaacaagaa cgtggtatta cgatcacttc tgctgctact acagcacaat
    27301 ggaagggtca ccgaatcaac atcatcgaca caccaggaca cgtagacttc acagttgaag
    27361 ttgaacgttc attgcgtgta cttgatggtg ctgtaactgt tcttgatgca caatcaggtg
    27421 ttgaacctca aactgaaaca gtttggcgtc aagcaacaac ttatggtgta cctcgtatcg
    27481 tatttgcaaa caaaatggac aagatcggtg ctgacttctt atattcagta tcaactttac
    27541 atgaccgttt gcaagcaaac gctcacccaa tccaattacc aattggtgcg gaagacgact
    27601 tcacaggaat tatcgacctt gtaacaatga aggctgaaat gtatacaaac gatcttggaa
    27661 cagagatcga agaaacagaa attcctgaag aataccgcga attagctgaa gaatggcgcg
    27721 aaaaattggt tgaagctgta gctgaaactg atgaagaact tactttgaaa tacttagaag
    27781 gtgaagaaat cactgaagct gagttaaaag aaggtatccg ccgcgcaaca gttaacgtag
    27841 aattctaccc agtactatgt ggttcagcat ttaagaacaa aggggttcaa ttactattgg
    27901 acgctgttct tgattacttg ccatcaccgt tagatatccc agctatcaaa gggattgacc
    27961 ctaaaactga tgaagaagta gaacgtcctg cagacgacag cgctcctttc tcagctttag
    28021 catttaaagt tatgactgac ccattcgtag gtcgtttaac tttcttccgt gtgtactctg
    28081 gtgttttaca atcaggttca tacgtacaaa acgcaacaaa aggcaaacgt gaacgtgttg
    28141 gtcgtatcct acagatgcat gccaactctc gttcagaaat ctctgaagtt tacgctggag
    28201 atatcgctgc tgctgttggt ttgaaagaca caactactgg ggacacacta tgtgatgaaa
    28261 agagtctagt tattcttgaa tccatggaat tcccagaacc agttatccaa gtcgctattg
    28321 aacctaaatc aaaagctgac caagataaaa tgggtgtggc tttacaaaaa ctatctgaag
    28381 aagatcctac tttccgtgct gaaacaaacg tggaaacagg cgaaacaatc atcgctggta
    28441 tgggtgaact tcacttagat atcatcgttg accgtatgcg tcgtgaattc aaagttgaag
    28501 ctaacgtagg tgcgcctcaa gtatcttacc gtgaaacttt ccgtgctggc actcaagccg
    28561 aaggtaagtt tgtacgtcag tctggtggta aaggtcaata cggtcacgta tggattgaat
    28621 ttacaccaaa tgaagaaggt gctggtttcg aatttgaaaa tgcgatcgtc ggtggggttg
    28681 ttcctcgtga atacattcca gctgttgaaa ctggtttgaa agatgctatg gaaaatggtg
    28741 tcttagctgg ctatccatta gtagatatca aagctaaact ttacgatggt tcataccatg
    28801 atgtcgattc aaatgaaact gccttccgtg ttgctgcttc tatggcatta cgtgcagcag
    28861 ctaagaaagc taatccagta atccttgaac caatcatggc tgtagaagtt gttatccctg
    28921 aagattattt aggtgatgtt atgggtcacg taactgctcg tcgtggacgt gttgaaggta
    28981 tggaagctcg cgctggcggc caacaagttg ttcgtgcgat ggttccactt gctgaaatgt
    29041 ttggttatgc tacaactctt cgttctgcta ctcaaggtcg tggtacattt acaatgacat
    29101 ttgaccacta cgaagatgta ccaaaatcag tacaagaaga aatcattaag aaaaacggcg
    29161 gaaaagctga ataatttctt gaaaccggat agtcctgcaa agagattatc caaagagact
    29221 atatttttaa tatagtttcg tgtaaaataa ttatcgatga tatgggcatg tggtgtacct
    29281 tgtgtacatc atatacctat cagacaaata aaaaatatat tgtattttta ggaggacatt
    29341 ttaaaatggc aaaagaaaaa tttgaccgtt ctaaaccaca cgttaacatt ggtactatcg
    29401 gacacgttga ccatggtaaa actacattaa cagctgcaat cacaactgta ctatctaaga
    29461 aaaacggcgg ccaagctatg gcttacgatc aaatcgatgg tgctccagaa gaacgcgaac
    29521 gcggaatcac aatctcaaca gctcacgttg agtatgaaac agatacacgc cactacgctc
    29581 acgttgactg cccaggacac gcggattacg ttaaaaacat gatcactggt gctgctcaaa
    29641 tggacggagc tatcttggta gtttctgctg ctgacggccc aatgcctcaa actcgtgaac
    29701 acatcctatt gtctcgtcaa gttggtgttc cttacatcgt tgtattcttg aacaaagtag
    29761 acatggttga tgacgaagaa ttactagaat tagttgaaat ggaagttcgt gacctattaa
    29821 cagaatacga attccctggt gacgatgttc ctgtagttgc tggatcagct ttgaaagctc
    29881 tagaaggcga cgcttcatac gaagaaaaaa ttcttgaatt aatggctgca gttgacgaat
    29941 acatcccaac tccagaacgt gacaacgaca aaccattcat gatgccagtt gaagacgtgt
    30001 tctcaattac tggacgtggt actgttgcta caggtcgtgt tgaacgtgga caagttcgcg
    30061 ttggtgacga agttgaagtt gttggtattg ctgaagaaac ttcaaaaaca acagttactg
    30121 gtgttgaaat gttccgtaaa ttgttagact acgctgaagc tggagacaac attggtgctt
    30181 tactacgtgg tgttgcacgt gaagacatcc aacgtggaca agttttagct aaaccaggta
    30241 caatcacacc tcatacaaaa ttctctgcag aagtatacgt gttgacaaaa gaagaaggtg
    30301 gacgtcatac tccattcttc actaactacc gtccacaatt ctacttccgt acaactgacg
    30361 taacaggtgt tgttgaatta ccagaaggaa ctgaaatggt tatgcctggc gacaacgtaa
    30421 caatggaagt tgaattaatc cacccaatcg ctatcgaaaa cggtactaaa ttctcaatcc
    30481 gtgaaggcgg acgtacagtt ggtgccggtg tcgttacaga aatcaaagct taattcactt
    30541 aagttttaaa gtaaagcaac atttattcgg acgtaatcgc tcggaacgaa ggtcttagga
    30601 tctttgttcc gagctttttt atttattgca ggatcaggaa aaaagggctc tttgtcaaat
    30661 aggactgata gagtgagata agaacatcta aggtagaatt ccttttctac tttagatgtt
    30721 ttttgattca taggtaatca gattatttag taagaaaatt ctggtcttca ttttccgaaa
    30781 gcttcgaaag ccataagcga ctcgtttcaa tgctttgatg tgggtatgca gattttctat
    30841 tttggcattt gaataaggca attcgagggc taaacgaatg ccggattcat agtttataaa
    30901 agcttttttt atagaatgca gttcttttgg aacggaattg ggcatcgttc ttacaagttt
    30961 aaagaaagtg tccgcctctt tgtcgtgaaa cgcttgaaga atttcttgat agtaaaaata
    31021 ggcgattcgt aattcgtcag agatcgaaag cagacgatcc accacttcac tttcggtgag
    31081 atacgcccaa ccaaaattgc gccatttggt ccatttgtca taaacaagct tgtcctgtcg
    31141 tttgatcaga agccgagaca acgcctttaa ttgtcgataa tatttggctt gtgttggatc
    31201 gtttcgatca aagcttttca tgattcgtac gcgaatatcc tcaaatttac gaatgagatg
    31261 tttgacaatg tgaaaacgat ccacaacgag ttttgccttt ggaaaacatt cctttatcag
    31321 agaggcataa gaaacattca tgtccgtcac gatgaccttt accttcaatc gtgcgcgtcg
    31381 ttcaaattgc ataaaataag aaacgagagc ctttttcttc cgtgaaggaa gaatatccag
    31441 taattttcca gtatcgccat ccatacaaga aaaggagtag ggaccgactt gatttttcag
    31501 tgaacgaaat tcatccatta ataaacaacg cggtaaccaa tttcttttta cttttgtcat
    31561 ggggatcaga gaagcgagtg tccgagaaac ggtcgaccaa gaaacgccgc aatgcttagc
    31621 aattgtcgtc attgcttgtt tttcagttag ctcttctaaa attttatagc gcacttgttt
    31681 agcgataaag cagtttctat cgacaatgta ggtattcgcc gtaaaatagg tgtggcagcc
    31741 acgacagtgg tagcgttgtt tcgctaaagc aagataagtc agttggttcg cacatttatt
    31801 aagttgaatg agcgattttt tctttccgtt cttaacgatc atgccttcat gattcatctg
    31861 tttacacaaa ggacagcgac gaatttcttt acttaacgtc gcagaaacaa caagactctt
    31921 gatattctta atgctcttcg attccacatt tgtgatgaaa atgtttttat ctttgattcc
    31981 aagtagtagt ttgatatgat gaagatcagg catacagaac gctcctttga ttgtgttgtg
    32041 gtgattcaat tctaacggag cttctgtatg ttttgttttt tcatgctcaa aagggaccga
    32101 tagaatgatt ttctatcagt cctagaaatt atagagccga ttttgaccga caggtataac
    32161 cctgccggtc tttttttgct gctacaggaa aactacagac tggctacagg gaaagtacag
    32221 atgcgcttgc cataataaca atcgtaccag ccacaaatcg tagttttatt gcaaaggagg
    32281 cattcgatca aatggaaaaa agcaactatc attccaatgc ggatcatcac aaacggcata
    32341 tgaaacaatc cgtggaaaag cgggcttttc tatgtgcgtt cattatttcg ttcacagtct
    32401 gcacgctgtt tttggggtgg agactggctt ccgtattgga ggcaacacag ataccgccca
    32461 tccctgcaac tcatacaggc agcagcactg acgtagtgga gaatttggag gaaaacgctc
    32521 ttgccaccgc caaagaacag ggagatgaac aggaatggag cctgatttta gtgaacaggc
    32581 agaaccccat ccccgcacag tacgatgtgg agcttgagca actatcaaat ggtgagcgga
    32641 tagatattcg gatttctccc tatcttcaag atttgtttga tgccgcaaga actgatggag
    32701 tttacccgat tgtcgcatcc ggataccgaa caacagaaaa acagcaagaa attatggatg
    32761 aaaaaattgc cgaatataag gcgaaaggct acacctctgc acaggctaaa gcggaagcag
    32821 aaacttgggt ggccgtgccg ggaacgagcg agcatcagct tggtcttgct gtggatatca
    32881 atgcggacgg aattcattca acaggcaacg aggtttatag atggctggat gaaaacagct
    32941 atcgttttgg ttttattcgt cgctacccac cagacaagac agagataacc ggtgtgagca
    33001 acgagccgtg gcattaccga tatgttggca tcgaagctgc cacagagatg tacaaccaag
    33061 gggtttgcct tgaggaatat ttaaaaccag aaaaatgaga aaaggatgtt atgttatgga
    33121 cagaaaaaga ttgacacagc gtttcccgtt cctgcttcca atgagacggg cgcagagaaa
    33181 aatgtgcttt tatgcgggaa tgagatttga cggctgtcgc tatgcgcaga cgatagggga
    33241 aaaatcgctt tcccatttgc tctttgaaac ggattgcgca ttatacaacc acaataccgg
    33301 atttgacatg atataccagg aaaacaaggt gttcaactta aagctggcag caaagacctt
    33361 aaacggctta ttgataagac cgggggaaac cttttctttc tggtggctgg tacgccatgc
    33421 ggacaaagat accccctata aagacggcct tacggtgacc aatggtaaac tcaccaccat
    33481 gtcgggcggc ggtatgtgcc agatgagcaa tttactattt tggatgttcc tgcatacgcc
    33541 attgacaatt atccagcgca gaggtcacga agtaaaggag tttccagagc caaacagtga
    33601 cgagattaaa ggggtggatg caaccatttc agagggctgg attgatttaa aagtgcgaaa
    33661 cgataccgac tgcacctacc aaatatgggt gaccttagat gatgagaaaa tcatcggtca
    33721 ggtgttcgcc gacaaagagc cccaagcatt atacaaaatt acaaatggca gtatccagta
    33781 tgtccgtgaa agtggcggga tttatgaata tgcccaggtt aaacggatgc aagttgcctt
    33841 aggtaccggg gaaataatag attgcaagct gctttataca aacaaatgca aaatctgtta
    33901 tcccctcccg gaaagtgtgg atattcagga ggagaaccaa tgagaaaaag tgtgggcatt
    33961 actgtttttg gatgcgaaca ggatgaggca aatgctttcc gcgctttatc gccggatttt
    34021 catattatcc ctacgctgat tagcgatgcg atatcggcag acaacgcaaa attggccgct
    34081 ggcaatcaat gcgttagcgt aagccataag tccgaggttt ccgaggcgac aattcttgcg
    34141 ctgagaaagg tcggggtaaa atacatttct acccgcagca tcggctgcga tcacattgat
    34201 acgactgccg ccgagagaat gggaatctcg gttggcacgg ttgcgtattc gccggacagc
    34261 gttgcggatt atgctttgat gctgatgctg atggccatac ggggtgcaaa acccaccatg
    34321 cacgccgtgg cgcaacaaga tttcagattg gatcgtatcc gggggaaaga actgggggat
    34381 atgactgtgg gagttattgg aaccggccat atcgggcaag cggtcgtcaa aaggctgcgg
    34441 ggatttggat gccatgtgct ggcctatgat aacagccgaa aaatggatgc agattatgtc
    34501 cagcttgatg agcttctaaa aaacagcgat attgttacgc tccatgtgcc gctttgtgcg
    34561 gatacccgcc atctgatcgg tcagaagcaa attggagaga tgaagcaagg cgcattttta
    34621 atcaacaccg ggcgcggagc gcttgtcgat accggggcgc tggtggaggc gttggaaagc
    34681 ggaaagctgg gtggtgcggc actggatgtg ttggagggcg aggatcagtt tgtttatacc
    34741 gattgctcgc agaaagtgct tgaccatccc tttttgtcgc agcttttaag gatgccgaat
    34801 gtgatcatca caccccatac ggcgtactac actgagcgtg tgctgcagga tactacagaa
    34861 aaaacaatca ggaattgtct caattttgaa aggagtttac agcatgaata gaataaaagt
    34921 cgcaatcatc ttcggcggtt gctcggagga acatgatgtg tcggtaaaag ccgcaataga
    34981 aattgctgcg aacattgata cggaaaaatt cgatccgcac tacatcggaa ttacaaaaaa
    35041 cggtgtatgg aagctatgca agaagccatg tacggaatgg gaagccgaca gtctccccgc
    35101 catactctcc ccggatagga aaacgcatgg gctgcttgtc atgaaagaaa gcgaatacga
    35161 aacacggcgt attgatgtgg ctttcccggt tttgcatggc aaatgcgggg aggatggtgc
    35221 gatacagggg ctgtttgtat tgtctggtat cccctatgtg ggctgtgata ttcaaagctc
    35281 cgcagcttgc atggacaaat cactggccta cattcttaca aaaaatgcgg gcatcgccgt
    35341 tcccgaattt caaatgattg ataaaggtga caagccggag gcgggtgcgc ttacctaccc
    35401 tgtctttgtg aagccggcac ggtcaggttc gtcctttggc gtaaccaaag taaacggtac
    35461 ggaagaactt aacgctgcga tagaagcggc aggacaatat gatggaaaaa tcttaattga
    35521 gcaagcgatt tcgggctgtg aggtcgggtg tgcggtcatg gggaacgagg atgatttgat
    35581 tgtcggcgaa gtggatcaaa tccggctgag ccacggtatc ttccgcatcc atcaggaaaa
    35641 cgagccggaa aaaggctcag aaaatgcgat gattacagtt cccgcagaca ttccggtcga
    35701 ggaacgaaat cgggtgcagg aaacggcaaa gaaagtatat cgggtgcttg gatgcagagg
    35761 gcttgcccgt gttgatcttt ttttgcagga ggatggcggc atcgttctaa atgaggtcaa
    35821 taccctgccc ggttttacat cgtacagccg ctacccacgt atgatggccg ccgcaggaat
    35881 cacgcttcct gcactgattg acagcctgat tacattggcg ttaaagaggt gataagtatg
    35941 gaaaatggtt ttttgttttt agatgaaatg ttgcatggcg tccgttggga tgccaagtac
    36001 gccacatggg ataacttcac aggaaaaccg gtggatgggt atgaggtgaa tcgcatcatc
    36061 ggcacaaagg ccgtggcgtt tgctctgcgc gaggcacaaa tccatgcggc tgcgcttggc
    36121 tatggcttgc ttttatggga tggatatcgg ccaagaactg cggtggactg cttcctgcgt
    36181 tgggcagcgc aaccggagga caatctcaca aaagaaaaat tttaccccaa tatagagcga
    36241 gccgagttga ttacaaaggg ttatgtggcc tcacaatcca gccatagccg tggaagcgca
    36301 attgatctta cgctctacca cctggataca ggggaacttg tttcaatggg aagtaacttc
    36361 gattttatgg acgaacggtc gcatcataca gcaaaaggga taggggatgc agaggcacaa
    36421 aatcgaagat gcttgcgtaa aatcatggaa agcagcggat ttcagtctta tcgctttgaa
    36481 tggtggcact ataagttgat tgatgagcca taccccgata cctattttaa ttttgccgtt
    36541 tcataatgaa agtattggtt ttgtatcaga ttttggattt gataaaatct atgctggtca
    36601 tggacaatct tttactggaa agtggttaaa gcatcttttg taatacattg caaattatag
    36661 tattcaatct gcccagcggc agaaaagaaa aaaaccgctg ttgggcagac ctttcccacg
    36721 cccattttgt attttgctgg cggcggtttt gagaaatcaa ggccgccgtt cttttctggc
    36781 agcaaaaggt gtggcggccc ataggaggat gccgctatgc tgttttgcta ttgccatgct
    36841 ctaatccact tccaccactc aaaaaaagcc ttgcccctc
//
LOCUS       DACCXM010000012        35222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCXM010000012 DACCXM010000000
VERSION     DACCXM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.012"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(76..1338)
                     /locus_tag="IVY61_000532"
     CDS             complement(76..1338)
                     /locus_tag="IVY61_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7969671.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1331..2230)
                     /locus_tag="IVY61_000533"
     CDS             complement(1331..2230)
                     /locus_tag="IVY61_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969672.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2375..4153)
                     /gene="uvrC"
                     /locus_tag="IVY61_000534"
     CDS             complement(2375..4153)
                     /gene="uvrC"
                     /locus_tag="IVY61_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP7969673.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4335..4649)
                     /gene="trxA"
                     /locus_tag="IVY61_000535"
     CDS             complement(4335..4649)
                     /gene="trxA"
                     /locus_tag="IVY61_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP7969674.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4736..7096)
                     /locus_tag="IVY61_000536"
     CDS             complement(4736..7096)
                     /locus_tag="IVY61_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7969675.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7211..7759)
                     /locus_tag="IVY61_000537"
     CDS             complement(7211..7759)
                     /locus_tag="IVY61_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP7969676.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7818..8255)
                     /gene="zapA"
                     /locus_tag="IVY61_000538"
     CDS             complement(7818..8255)
                     /gene="zapA"
                     /locus_tag="IVY61_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP7969677.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8457..9374
                     /locus_tag="IVY61_000539"
     CDS             8457..9374
                     /locus_tag="IVY61_000539"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP7969678.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9462..10373)
                     /locus_tag="IVY61_000540"
     CDS             complement(9462..10373)
                     /locus_tag="IVY61_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7969679.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10774..11352)
                     /locus_tag="IVY61_000541"
     CDS             complement(10774..11352)
                     /locus_tag="IVY61_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7969680.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11372..12040)
                     /locus_tag="IVY61_000542"
     CDS             complement(11372..12040)
                     /locus_tag="IVY61_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969681.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12055..13125)
                     /locus_tag="IVY61_000543"
     CDS             complement(12055..13125)
                     /locus_tag="IVY61_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7969682.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13296..14660)
                     /locus_tag="IVY61_000544"
     CDS             complement(13296..14660)
                     /locus_tag="IVY61_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7969683.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14657..16216)
                     /locus_tag="IVY61_000545"
     CDS             complement(14657..16216)
                     /locus_tag="IVY61_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7969684.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16272..18752)
                     /locus_tag="IVY61_000546"
     CDS             complement(16272..18752)
                     /locus_tag="IVY61_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969685.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            complement(18736..19071)
                     /locus_tag="IVY61_000547"
     CDS             complement(18736..19071)
                     /locus_tag="IVY61_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969686.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19085..20119)
                     /locus_tag="IVY61_000548"
     CDS             complement(19085..20119)
                     /locus_tag="IVY61_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7969687.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20209..20889)
                     /locus_tag="IVY61_000549"
     CDS             complement(20209..20889)
                     /locus_tag="IVY61_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7969688.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(21173..22276)
                     /locus_tag="IVY61_000550"
     CDS             complement(21173..22276)
                     /locus_tag="IVY61_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7969689.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(22498..22968)
                     /locus_tag="IVY61_000551"
     CDS             complement(22498..22968)
                     /locus_tag="IVY61_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP7969690.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            23301..24941
                     /locus_tag="IVY61_000552"
     CDS             23301..24941
                     /locus_tag="IVY61_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP7969691.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            25095..26417
                     /locus_tag="IVY61_000553"
     CDS             25095..26417
                     /locus_tag="IVY61_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP7969692.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(26479..26910)
                     /locus_tag="IVY61_000554"
     CDS             complement(26479..26910)
                     /locus_tag="IVY61_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7969693.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(26974..27810)
                     /locus_tag="IVY61_000555"
     CDS             complement(26974..27810)
                     /locus_tag="IVY61_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7969694.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(28552..29178)
                     /locus_tag="IVY61_000556"
     CDS             complement(28552..29178)
                     /locus_tag="IVY61_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969695.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(29473..29808)
                     /locus_tag="IVY61_000557"
     CDS             complement(29473..29808)
                     /locus_tag="IVY61_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7969696.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(29795..30079)
                     /locus_tag="IVY61_000558"
     CDS             complement(29795..30079)
                     /locus_tag="IVY61_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP7969697.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(30135..31658)
                     /locus_tag="IVY61_000559"
     CDS             complement(30135..31658)
                     /locus_tag="IVY61_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7969698.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(31651..32826)
                     /locus_tag="IVY61_000560"
     CDS             complement(31651..32826)
                     /locus_tag="IVY61_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7969699.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(32830..33015)
                     /locus_tag="IVY61_000561"
     CDS             complement(32830..33015)
                     /locus_tag="IVY61_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969700.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(32981..34675)
                     /locus_tag="IVY61_000562"
     CDS             complement(32981..34675)
                     /locus_tag="IVY61_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7969701.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(34672..35145)
                     /locus_tag="IVY61_000563"
     CDS             complement(34672..35145)
                     /locus_tag="IVY61_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP7969702.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
BASE COUNT        10090 a         7337 c         6028 g        11767 t
ORIGIN      
        1 taaataaaat aggtctatac aaaaagaagc tgggaacaat agatgtccag cttccttctg
       61 tattttagga ttgttttatg ctttttttcg ttcattcctc ataattgaaa tcgattgttt
      121 caatgaactc cataatccgc cttcgctata aacgagtaca ttcgatttgt acatgtttgc
      181 agacaagatc atcaataaga atgttacggc tacaagtacc aacaaggaaa tcaatgctcc
      241 acccataccg actgtctcat ttgctaagcg aaccggcata atatatgaag ataagaatgg
      301 aatatacgaa gtgatccgaa taacgatatt gtttggatct gaagctccta gtatcaatcc
      361 aagcatgtat ccgcctagag atagataagt cactggcaaa attgcttttg ctgtatcttc
      421 tgctttgttg accaaagagc cacataaagc tgctaaaaca gaaaaaatca aaatgccaaa
      481 gatcatgaat agaagagaat accacaagaa tggtccaatc aagttatcca atgagatacc
      541 gtctaatata tttttgacca tttccatatc tttgaattga ctgtatccta taccaaaaat
      601 aataccgtat aatgccattt gcgttaatgc aacaagtaaa acacctgtta attttccgta
      661 aaaatgcttt tgtgcagtcg tactggacag gatcacttcc atgatccgcg tgcctttttc
      721 agaagcgatc tcttgtgcaa tgatctgcgc atacgtcaaa ataataatga acaagacaat
      781 cgttgctaca taacttacga cgtattggac tgtcgagtta tcttctccga ctgtcatttt
      841 tccttttgaa tcaaaattga ctttttgtcg agtcaaactt gcttgttggc tcaagctagc
      901 tacttcttct ggtgaaatgc ctaattgact cgctcgaagc atcgattgca agccagtcag
      961 ttgttgttgt atcagtagtt gtgtactttg tcctaatgaa ttttcgctat aaagcatccc
     1021 atttatctta tcattctcag ttgagactac taaatacgca tcgatttttt cttccgaaag
     1081 ctctttttgt gctttttctt cagaatcaac tgctttgaac tcaaaatcat ctgttttgac
     1141 ttggctcatt tgctgactga tttgctcatt atcagccaat acgccgattt tattgacatc
     1201 actattttgt tgagcaaagt ttcctgctac ataaatgata cccagcatga taaaagggac
     1261 taatatcatg atcaagaaag aaatcgactt aacatttttt aaatagacat ctttggtaat
     1321 aatccaaaac ttacgcatcg atttcacctg ctttcaattt gaaaatctct tccaaagttg
     1381 gcggttgctg gttaaacata ggaatgtagc cgaactgagt cgcacgagta aagatttctt
     1441 tcccagcttc aggatcttct aaggtaatct ctaacgtatt gtcttcatga aggatcactt
     1501 ttttcacgcc ttcgatttct tctacttcct gttggcttaa accagattcc aagaataact
     1561 tcgtacggcc aaatgattca cgaatctcat gtacttttcc attcaaaacc atttttccgt
     1621 ttcgcaacat gatcaaatga tcgcagattt tttctacatt atccatattg tggctagaaa
     1681 aaatgacaca tgaaccatgt tttttcaatt ccaatattcc atcttttagc aattcagcat
     1741 ttaccggatc taacccgctg aatggctcat ccaaaatgat caatttaggt tcatggatta
     1801 acgtggcgat cagctggact ttttgctgat ttcctttaga caaagatttt actttatccg
     1861 tctttttccc ttttacctga aatttttcca tccaaaagtc gatttttggt tcaatctctt
     1921 ttcttgattt ccctctaagt tctgcaaaat aaagcagttg gtcttgaatc gttactttcg
     1981 gataaagtcc ccgttcctcc ggaagataac cgatgacatc ataatctttc tccccaagat
     2041 gatggccatt ccataacact tctccttggt cttgtgtcaa aaaatccaag atcaaacgaa
     2101 aagtcgttgt cttcccagcc ccgttttggc caattagccc catgattttt ccatcgggga
     2161 tttggaaaga gacatgatcg acagctgtgt agttcccaaa agttttaaca agatccttga
     2221 cttctaacat ttcttcactc cttcttcaag aaatcacata tatccttaga caccagacgt
     2281 aagcgattta ttatttacca acaaaacgta aacgctcttt ttgttgtaca taaaatgata
     2341 ggacacttta ctttctttgt aaagtgccct ttcctcattc ctgatgtaat ttatcaaata
     2401 cgttttgtgc gacatttttc ggcagtccga tttcttgtaa ttcttctgtc gtggctgctg
     2461 tgatattttt caacgatttg aactctttaa gcagcatttt ttttcgtttt ggtcctaacc
     2521 cttcaatttc atctaatcgg gaagcaaaac tgtttttact gcgtagctgc cgatggaaag
     2581 tgattgcgaa tcgatggact tcatcttgga ttcgctggag taagaaaaac tctggtgaat
     2641 ttcgctcaag cggtactaca gccaaatctg taccgaatag caactcattt gttttatgtt
     2701 tatcgttttt tgccatgcca gcaataggaa tatcgattcc taattgattt tctaagacat
     2761 cttttgccac atctacttgt ccttttcctc catcgatcaa gatcaaatca ggtaaaggca
     2821 acccttcgcg aatcactcgc gaatatctgc gatagatgac ttctctcatt gatgcataat
     2881 catccggtcc gttgactgtt tttattttat atttccgata ttcttttctt gccggttttc
     2941 catcaatgaa tacgaccatc gcagccacag gattcgtccc catgatatta gagttatcaa
     3001 aagactcgat acgtattggg gtcgggatat tcatcgcgtt tcctaaacgt tcaactgctc
     3061 cgatcgtccg ttcctgtttt ctggcaatca gatcaaaccg ttcattcaaa gcaactgagg
     3121 cattcttact cgccaattca actaattttt tcttttctcc tcgttttggt tgcagcactt
     3181 tcgtagctaa caaagcttct acactgggct tgtcgatcgt atcaggaatc aagacttctt
     3241 taggaatgaa atgctcattt tcctgataaa actgaccgag gtaagtcaag aaatcttctt
     3301 ctgcttcgtt atagaaagga aacattgaaa catctcgttc aattagcttt ccttgtctta
     3361 cgaaaaacac ttggacacac atccatcctt tatcaatcgc ataaccaaat acatctcgat
     3421 ccagtaaatc agtatttgtc attttttggc gagtcatgat tgtctcaatg gcacggatct
     3481 ggtctcggta ttctgcagct ttttcaaact ccatgttttc agcagctttt tccattttct
     3541 gatggatctc tgactcgatt ttttcgtgtc ctccgtttaa aaagtttttc acttcatcaa
     3601 cgattttcgt atagactttc ggatctacgt cgaaataata aggacacaga cattgtccca
     3661 aatgatagta aagacaaggc tccttcgtct gactaggttt gcattttctc agaggaaata
     3721 atcgatcgag gatcttcttt gtctcattcg ccgcaccgac atcgggatat ggtccaaaat
     3781 acagtgcttt gtcttttagt accttacgag taatcaccaa acggggatat ttttcattcg
     3841 ttatcttaag gaaaggatag gttttatcat cctttaacat aatgttgtat ttaggatcat
     3901 ttttctttat cagattgatc tctaagagca atgcttcgat attcgattct gtaacgatgt
     3961 attcaaagtc ttcgatttca ctaacaagcc gttccgtttt cgtattatgg ctaccagtaa
     4021 aatatgagcg gacacgattt tttaatattt tggctttgcc gacataaatg atcgttccat
     4081 ttttatcttt catcaagtaa cagccgggct gatcaggaag taaagctaat ttgtttttga
     4141 ttcgttcgtt catctggtac tccccttcat attcaattaa agttattata gcatgtctgt
     4201 caaaaaaaca atccagcctg acaacgtaat tatactcaaa aaatattaat ttgaataacc
     4261 ttaactcaac cgcaaaaaaa ccgaggacgg aactggattc agtcccaccc tcagcttatt
     4321 tttgctgtaa gtttttataa atattgatcg atcatttgac gtagttgttc ttttgaatga
     4381 acaccaacag ctttttctac tacttgacca tcttttttca acattaatgt tggaatgctc
     4441 atgataccga attgttgagg agtttctgga ttttcatcca catccatttt agcaattctg
     4501 aattcttctt catcatattc ttgttccaac tgatccaaaa tcggtgcttg catacgacat
     4561 ggaccacacc aagttgccca aaaatcaatc aatacaagtc ctttatctgt ttcttcatta
     4621 aatgtcttat ctgtaattac ttgtgacata tcaaagacct ccatttttaa aatctatgac
     4681 tagtatagca tttctatact tggatagcga accatctgct tacctttagt aagctttact
     4741 taaatttaac aattgtcgca ccatttccgc cttggtttgc cggtgcaaac tcaaagcttt
     4801 tgacgctccg atggttttta agataatcag taattccctt gcgaagtgcg cctgttcctt
     4861 ttccgtggac aatagtcact tgaggataac cagctagaat ggcagaatcc aaatattgat
     4921 ctacctcact tagcgcttct tcgtatcgtt ttcctcgaag gtccaattga ttcggcacgt
     4981 gactactaga ttcagatcgt acagcatgga taactcgttg tgtcggctct ttttgaggag
     5041 caacaggcgt cagctcgtct tcggacacat tcatttttaa tataccgatt tctacttgcc
     5101 actgattccc gtttttacgt aaaagcgtcc ctctttggcc gtaagtcgta accaacactt
     5161 cgtctcccgg tttcaacgtt ttttgttctt tcgccttctt caatacttta tttttcttca
     5221 aagtttcttc ttgatgtaga tttgctaatt gggtctttgc atcgatcaac tgatgctctt
     5281 tcacatttcc ttgcccaatc tgctgttgca ttttacgaat atcagcaatg attttttctg
     5341 ctttttcttc cgcctctgac acgacttcat ttgctttttt ctttgctttt tccatttctt
     5401 tttcacgttc ttcaaagaaa tagctgtaag cttctttcaa atcatcgtga agttcttgtg
     5461 cctcagaaac aaaatgacgc atttccagat attcggtctc tgccattttc cggcgattct
     5521 ctaaatctgt gatcatttcg tttaaatctt ggctctcatc attcatcaac tgcttcgctt
     5581 catcaataac ttctgtatca agacctaatc ggctagagat ttcaaacgca ttgcttcgtc
     5641 caggaacgcc aatcagcaaa cgatacgttg gacttaatgt atccacatca aactccatgc
     5701 tggcattaat cgtatttgcc cgattatatc cataaacttt caattcagga taatgcgttg
     5761 ttgccatcac ataagcagac tttttaccaa gatcatctaa gattgcgatt gctagagcag
     5821 caccttcttg cggatctgtt cctgcaccga gctcgtcaaa taataccaga cttttttcat
     5881 tgacttttga taaaatatct acagtgttcg tcatatgaga agaaaaggtc gatagacttt
     5941 gctcaatcga ctgttcgtct cctatatccg caaatacttc ctcaaaaata ccgatttgac
     6001 tttcttcacc tgcaggaatc ggtaatcctg cttgccccat cagttgtaac agacctaaag
     6061 tcttcaacgt gatcgttttc ccacctgtgt taggtcctgt aatgacgatg gcttgatagt
     6121 cttttccgat tgtgatgtca ttagaaacta ctttttcctg atcgatcagt ggatgtcttg
     6181 cttgtttcaa gacgacatga ttgtcttcac tgattcccgg cacgatagct tttacttctt
     6241 taccaaatcg tgcttttgca ttcatcagat ccattttacc aatcacataa gcattgtgga
     6301 tgatttcttg acgatgggga acaagttcag cagaaagttc acttaaaata cgttgaatct
     6361 catttcgttc agcgatttgg tattgacgca aacgattatt caaatccacc acttgttttg
     6421 gttcaataaa taaagtctgt cctgaagcac tttgatcgtg gactacaccg ccaaatacac
     6481 cgcggtattc ttgtttgact gggataacgt agcggtcatt tcgcatggtg atgatcgcat
     6541 cacttaaata ctttgcattt ttcccacgga cgatcccatc taactgttca cgaacagctt
     6601 gttcacttcg ccgaatgttc tgtcttatgc tctttaactc aggagaagcg tcatcagtca
     6661 cgcgtccgtc ttcatcgatt gcttccttta aacggcggct caaaacaggg atcgtgacta
     6721 actgatctgc ccacatatac aaacgtgcaa attcgatttc actgtcataa agatcatcaa
     6781 tgaaacgatt caattcgctt gtcgtagaca atactctgga aacttgtgca agctccactc
     6841 cattcaagtc tgctccgatc tcaatccgtt tcatatgggg acggatattt tcaagtttag
     6901 gtatagggat cccgcctcgc agacgttgca ctttcaatcc atcctcagtt tcttgaagcc
     6961 agtttgtgat cgtttgttcc tcgctcactg gtaccaattc tgccagttct tcttttcctt
     7021 gtgcagtcac tacaaattgt ctgaccatct gtttgacttt ttcaaattcc aatgtttcaa
     7081 ggattcggtt attcatttta tcaccttatc tttcactatg aaaaaagagc tgtgccggac
     7141 atacgctacg cagccttctt aaataatcta ctctgtacaa aaaacgaaat attttccagc
     7201 actattttcc ttaattcaaa atattcgtta cccaaagctc atacatcttg ttggtaagaa
     7261 atggcgttct ctcaacgata aagcgagcga aactatgctc gccgaattga ttttggacca
     7321 catctaatgg aacaaaggca agtaactgca aaatcaaaaa tatagtaacg taagccatga
     7381 ttagtgacag gatgcctcct gcaatccagt ctacctgtct caagatcggc acatacgtca
     7441 atccatggac aaaaaccccc acaaatcggg taagcagcca gccaattagc aaaataatca
     7501 gaaaagcagt tcctgcataa aaagcttcat ctaggtgaaa ggcaaatacc tgatcaaaaa
     7561 atgctaactt tgatgttggc gtgacagctg ggtatggaat gtataattcc aaatgacttg
     7621 ctaatggttt gtaaaaatgc tgggcagcaa taaatgacaa tacatatcca atcgcgaata
     7681 tgatttgcaa agcaaacccg cgacgtgctc cagaaaaaaa cgcaattagc aataaaaata
     7741 aaatcagcaa actcagcatg ctacgttcct ttctcttcta acttttttta aatttttctt
     7801 attttgataa aaggatctta attagaagat ttttgtttaa tttgtccttt tcgtttctcg
     7861 tttaaaatct gttgtgcttc cacatgattt ttaatggtat gatctgcttg accattttct
     7921 ttcaatactt cccgagcttc gttctcgatt gcttcaatgc gtttcacacg attttcaagc
     7981 tctgtaaatt tgatcatttt tttctttaat tctgctgttt cttcttccag ctccaagatt
     8041 ctttcttgtt tcttcaattg atccgaaagc gcattgacag ccatcaagat tgctgcttgt
     8101 tcattatcca tttgtggtga taattgtttc aattctgcta actgttcatt gatcagcttc
     8161 gttacgatat ccatatgatg cttggtttcc cgtccgataa tagtatatgt ttggttggca
     8221 atcaccgctt tgtagcgtgt tttttcgtgc gccatacgaa tcatttcctc ctatttgatg
     8281 aaagttttat ttagtgtacc atgaagttgg ttctttcacc gttctccaca aatatttttc
     8341 acatacttaa ctattatatg ataaacttta aaaaaatgct atgtctcgca atctcttaga
     8401 aaacgaaaag tcgtgagttg cggtatagtt ttatgtataa aaacgaggag aattttatga
     8461 gcaacgtcat tatcaaagca acaaacgacc aacttagtca aatgaaagca tactatcgct
     8521 cttatttgtt aaataaacag attccttata ctagctttgc tgcaaaaaaa gacgggacga
     8581 ccattaccgc ttatacatcc ggaaaagtga tgtttcaagg tacgaatgca gcaatagaag
     8641 ctgataaatg gggggaggca caggataaac aagccaagcc agcaaaaaca gcctcttctt
     8701 tgccaaaaaa tatcagtcaa ttatccgttt taggtagtga tgaggtcgga aatggcagct
     8761 atttcggtcc gctaacagta tgtgctgctt atgtgaaacg tgatcagctt gcagaattac
     8821 gtaaacttgg tgttagagat tcaaaagagt taaaagatag ccaaatcatc cagcttgctg
     8881 acatcttaaa aaaaaccatc ccctataaac tgcttgtctt atctccagaa aaatacaatg
     8941 agatccaacc aaaatacaat gccgttcgga tgaaagtcgc tttgcacaat caagcgatcc
     9001 atttgctgct aaaaaaaatc gagccagaaa aaccagaagc gattttgatc gatcagttta
     9061 cgccggaagc aaattttaaa aaatatgcgc gtttagaaaa aaatcaatta caagaaaaac
     9121 tgtacttcgt tactaaagga gaacaatatc atttagctgt tgctgctgct tctatcatca
     9181 gtcgtgccag ctttttggaa gagttacaca aagcatcagc tgaagctgga atcacccttc
     9241 catcaggagc tggaacgaaa tctgaccaag tagcggcaaa actattggag aaaggcggca
     9301 tgcctatgct tgaaaaatat gccaaacttc actttgcaaa tacagaaaaa gctttgaagc
     9361 tactaaaaaa ataaacggtg ttcaaaagct tgcatctgcg ataataagcc cggccaaacg
     9421 aaaaatatga ggagctattt ttgtttggct gggcttattt attactgaaa ataagtggtc
     9481 aatgcttgtg tcaaacttgc agcaatctgc tgctgataac tgtctgttac taattccttt
     9541 aaatcactat cattattcat ataacccatc tcgattaaaa tagaaggttg aagattttct
     9601 cgaagaactt gatagttccc agtggagatt cctcttgaat ccagaggcag atcagtgaag
     9661 gagtcagcaa ttgtctgtgc taatgtttcc tcttgtccat agtaataata tgtcgtgatt
     9721 ccagttgctt cattcgcata ttccgtagag tctgcgtgta aactgataaa gacatctgcc
     9781 tttttttctt cactgtagtc gcatatatct ccaagctgta ctaattcatc tgtagtatgg
     9841 gtcaaataaa ccgttgctcc agcatcctct agcgcctttt ttacttttat ggctgtactt
     9901 aatgttatat ctttttcttc tgtttgttca tcattgctta atgccccagt atcatttccg
     9961 ccatgcccag gatctaatac aatcactgca tcagatagct cttccggttt tgtttgtata
    10021 ttctttgtga ctttggttcc atcttcgagg gctaaatatc cagtatagcc atcatttgtc
    10081 ttgatctgat aagcattttt tgtttcagaa agaaactccg ccagctctcc gccgtttata
    10141 gttgcaagtg gggtagttag cgagtcatct gtatatagtg tacggtcatt ttttccgatt
    10201 ttgattaatg tctcttgttc tgtatcatca aatgattcct ttgtctttgt aaccgtactg
    10261 ttcgtactgc tgaccgtttg tccaactatc tttttttgac caaaaacatc tatttgaaaa
    10321 cagataataa tgataatgat tgcaaataca aaagctccaa ttattttttt cattcttgac
    10381 tctcacctcg tagatccaga ctaagggcca aaacttaaaa tcagcttaag taagcaaaaa
    10441 aggggctgta aaagatataa ccgtagaaaa taacattact gctaaaatga tttttttcat
    10501 ttttggcata tcctgaaatc tccttttgac tattcgtggt gattcatcta cccaataaat
    10561 agtttccttc attctacaaa caattaattt cttcgttatt aaattaaaac acttcctgga
    10621 acaccgacat aataaataaa aatataccct gataatcatg agagaataac aaatttgtct
    10681 ttatgaaaaa ctacctatat aaagaagcaa gatataacga tcttcgtcat atcttgcttc
    10741 ttcacctaac tattttctcc ctatataaca gctttacgct tctcctaata caccattcaa
    10801 atataaatcg atcaattgcg ccacatttag cggttcttcc acaggtcttt tcgaccgagt
    10861 gatgtaaggt gctaacacta agaaaaaatg ccttgctgcg atttcttttg aaatcccatt
    10921 tcttagcaca gattggtttg ctgaaaagat ttcttcaaaa ggttcacgat aatttttctt
    10981 ccatagaaag aaaagatgat ctcttgtttc ctttgaaaga ttgctttcaa gatcgtgaag
    11041 catcagaaac aaatcgacca gatgtgtctc aatcaaaaac tgcgccattt ttgtcaaggt
    11101 tacttgaaat cccgtttcgc ttgtttgaac gatttctctc aaagcttttc caacatcttt
    11161 taagtggttt tctaaaactg ccgtatatag tttttcttta tcaccgaaat aatgatagag
    11221 attgggctgg gtgatcccaa ctttttgtgc gatttctctt gttgaagtgc cgagatatcc
    11281 cttggacata aacaagtcag tcgcttcttt caaaattttt tgctgtgtat ctttgttgta
    11341 tcgatggacc atttttccac ctgctttttg gttattcttt ttctttcaat acaccatctt
    11401 ccatcataaa tattctatcg catttgtcaa tcagccgtgt atcgtgagta accatgatcg
    11461 ttgctttatt tttgtctttt gattcttttg ccaagatatc gactacttct cgtgcctttt
    11521 ctgaatccaa actagcagtt ggttcatctg ctagaataat ggacggatca ttgtacaatg
    11581 cacgagcgat tgcgaccctt tgccgttctc caccagatag ttcttctgga tacttgttga
    11641 tcaacttatc cacgccaagc tgctcaaata attcgtttgc ttctttttga cgatgttcat
    11701 gtttaatctt atctactaat aataactgct tttttactgt cagaaaagga accagattgg
    11761 aagcttgcaa aataaagcca atttctttaa aacgtagttt aacgcgttcg ctttcttttt
    11821 gattactgaa ttcttgatca ttgataaata cagagccact gctaggcgtc tgcaatccgc
    11881 ctgccaaagt caaaaaagta cttttacctg aaccagaagg accgatgatt gcaacaaatt
    11941 cccctttatc gatactcatg gttgtttctt tcaatgcttt aactacttgg tcgccatctt
    12001 ggaaactttt ttctacttct ttaaattcta ttgctttcat gttcttcctc cttcttatcc
    12061 aattgccttc aacggatcga tttttacgat tgtccgaaca gaaaatagtg cgcctaacac
    12121 agctactact aacatcaaca agctaatgac gccaaaaaac agccaattgc tttggaatgg
    12181 aaccgcatca ggtagaacaa gggatgttcc cactgttcct aatagaccaa gaagaatacc
    12241 aacaaatgat aaaatgaatg tttgtgccac tacagaacgc gcaataaatc ctcctgtgat
    12301 cccttgtgct ttcattacac caaagatatt aatcttttgc atcgtcagta cataaatgaa
    12361 aatcccgatc acaatagccg caataacgat caaaaatccg atcataaatc caaaagtcaa
    12421 tacttgcgca ttatatccag gtaattcatt gatgaattta gaaattttga tctgttctaa
    12481 atcttcaggt aaattactga ttttacctct gacaactatt gcgttaatcc gtgcattttc
    12541 acttgtatct tctttttcga agcggatttc ttggtaagca ttgatggttg tatagagaac
    12601 tggtgagaca ttgaatttgg catgatccgt aaagccagtt agtttaaacg tcttgtcact
    12661 accagataac ttaactgtat ctcctatggc tagaccatat tcttctttca aactaatatc
    12721 gcctactgcc tcatcatcgt tatcaaaagc ttttccttca acaagattgg gcatgatgaa
    12781 ttgattttta tcgataccga agaagctaac attgattttt ccttcttctg tactgtcttt
    12841 actagtcacg actccaggtg tttgggcaag atacgccact ttcccacctt cgacttcttc
    12901 tgctatcttc ttagtaatca tggacatccc tagatttgaa tttgcatctt tcgaaagcac
    12961 aatcgaatct gcctcccact tgtcgactgc tgttcgatta tcttgagcta agccataagc
    13021 taacccagtc aaaaaaaata ccaggtaggc gatcaaaaac atcacccctg caactaacgc
    13081 ataacgtaac tttgaatgca taatttcatt taatgctaaa aacatttaac tcctcctcct
    13141 gaaataatca ttttatacaa caattttatc acttgataaa tttttggcga atattatgct
    13201 caaaaaattc ttccgctatt tcattatatg aattttttat tctcgattga ttaatattta
    13261 ttatatcccc acacaataaa agaccgtgat atttctcaca gtcttttatc tactctattg
    13321 ctatttgctc tcttagctag tactttaaaa gctaactcat tgaggtagtg aaaacgagag
    13381 gtgtattcag acagaaatcc aaaatcctca actgctagct gtggaatcgt agttttgtgt
    13441 ttcacgatat ccgcaagcag gttcaaatct gataataaga attcttggct tatcagctgt
    13501 ccgccaatta tttctaatgt atccggatgg tagatcagct ttgcatttac tgttttgttt
    13561 tcttcgtcgt attgtggaaa tagatcaaat tctcctgaac acgaaccggt attttgttcc
    13621 aagaatggcg cttcatcttc tgtcagccct acagttccta aaaagtagtc tataatgact
    13681 gctgctgtta tcttttgtct gaggtcgtaa gcggttggct gaccactcaa agttagcgct
    13741 actgctcttg ccatatgtac agctcgagta actaaaggta aaaagagatg ccgtttattt
    13801 ttgtcaaaac tgactgggac gatatctcca atcgcataga tgtccggatc acttgtctgc
    13861 agatattcat tgactatcac agtctcatcc tcatacaaag ccactttttc ttttaacaat
    13921 gtcgtattag gacgagtttg ggcagaaaaa atgaccatat ctgcctttat tgtttgagac
    13981 agaagttgta cggatactac tttctcttct gattttttcc aatcgatcaa gaattcattt
    14041 ggataaaaat ttatttgctt agtttgcagc tgattttgaa aactgttgat catatcctta
    14101 tcgaaatatc ttgataaaac attcggcata taatcaacta gatgaataat gtatccttta
    14161 tttctcaaag catctgctaa ttccaatcca atgtaaccag caccgaagat cactacttct
    14221 tttgcttctt ttaaagcggc aagtacttgc ttagattgca ctagattttt ataggtgtag
    14281 acattttcag cttcaatttg tttatacaag gtgacaggag aggaacctgt cgctacgatt
    14341 aatttatcat aggttatggt ctcgtttttt ccttctttgg atgcgaggat gagctgatgt
    14401 ttgtctggat caagttcagt tactctccat tcggtgatca gttttgcccc actttcttga
    14461 agagtccgca tgttatgggc aacttcagct aaactacgta tctttccttt taaataaagg
    14521 ttgattccat ttgaaacata gcctagtcca tctttatgat cttcatcgat cagtaaaact
    14581 tctgctttcg gttgaagttt tcttagattt agggcagctg tgatgcctgc atgggaagca
    14641 ccaatgatca cgattttcat gttttattca tccttaaatt caatttttct aaataacatt
    14701 cttttaaaaa ttggttcaaa taggggaaat cataattata ttcgttgcta gtgaatacaa
    14761 aaactttagc ggacttctgt gacgggtact ctttcaattg gctagtcaaa atgatatcat
    14821 actgcttttc tttctcgtat tcgactaaat catatttgat cgtattcagc aattgttcct
    14881 ggatgatttc tttcacgata tccgccatta gcctttcata aggaagatca taagcaattt
    14941 gcagacgtaa ttctaactgc ttgatcagcg tccgtaaaat agatgcatag cgagcaaata
    15001 attcaaagcg ggcattttcc tcgtctactt cagtaaaatc aacaatagca aatgtttgtc
    15061 tgaccaatct ttcacataaa accaggaatt gtttatcttc tacttcagtt aaccgctgtt
    15121 ttaatccttc ttggccaaaa atattcatcc atcctctaaa ataggcaaaa tgagcgtgat
    15181 tttgcatcac tgtcaacgca atcttttgcg aaatctcttc tggaaaaaca taaaaagaga
    15241 aatattcttc tatcgattgt ttaaacgttc gattcaattg agctactacg tcgtctttgt
    15301 atttttcgct tatcaaaaac tcctggacat gcgagttatg tgtatctaat gtaaagttac
    15361 tgatcatgaa aagataaaaa accaacgttt cattttgatt ccaaggaaag gcatattgaa
    15421 agaagtaacg ggtcaaaaat gtatggaggt ccaataaaaa ttgtgtcgtt tgaaacaatc
    15481 caacaggaag attgctataa tctggtttgg attttttcgt acagcgcatt cgcttcttca
    15541 taatggaaag ccatatttca aatttcaatc gtccttcttc tgtaatagca aaactaaact
    15601 ctttttgcag cagatctaaa aaagtgtttc ctgataaatc actgtattct tttttgtttt
    15661 caagataggg acgtccaaac caaaataatt ggaaataaaa ataacgaatt tgactttctg
    15721 gaccggtcag ccgcccgctt tttattttca gatgaaactc atttaaagaa tcatttaatt
    15781 ccttcactct gcgagacaaa gtagccgaac tgatattaag ttccatgaca agtttctgcc
    15841 gatcaaatga gccattttcc aacagataaa gaagtatctg atattttgta gaatagcgca
    15901 agtaacaatg ataataaaca tcgactgctg ctatatttcc atgtctgatt aataaaatat
    15961 tcccttcttg gataacaatt tccaattgtt tgatattttc caagttagct tgtaaataaa
    16021 acagatcttc tttcaaggta gcccgatcaa ctgataagcg ggtaagtacg ttattaacgg
    16081 aaacctctcc tttttcctct aataaaagtt ggaaaagttt gatttgccgt tgttcatttt
    16141 tttcaagcaa actgaaaagt tccaagtcca ctccttcaag catttctgat tttaacagca
    16201 gaagtttcct tctcatcctt gaaaaagagg atctgtgaca gttatcacag atccatacaa
    16261 attctaatca cttagctggt tggcccagct gtcagtttcc atgtgaccat gccttgatac
    16321 gttgcgccag gttgagcatt cgtactgcta tttacctcta agagtaaacc atcggtatcg
    16381 ggaaagtcaa cgagaacctc tcctttagct gttccatcac tctcataaat caacgtgttt
    16441 tgaccttgct gaatcaattg ctttgatcca gaatgattga agtataattt tgttccgctt
    16501 aattcttgac cttgatcatt cttaaataaa gaagtcaatg tacctgtcag ttgccaagga
    16561 gacgtgcttg gaagccgagt atcccgcaca tcaaaagaaa aatcgccttt tgattcataa
    16621 taacttgtct tcaacggttt ggggagatcg ttaccaaatg taaaatcatc tggtacatta
    16681 actaatgtta attcttgttt ggggacaaga attgttttag cagatgtttg gacaactggg
    16741 ttaggaagct tagcaccatc tgctaatgta atattgctgg aaagtacagc tgcctttgtc
    16801 agtatgctga cggattgtaa agaagtatct acagtattta attgatagtt gtaagtaaga
    16861 tgaatggtat ctcctatttt gctcaataga tctgctggga cagaaactcc ttgttgaatg
    16921 ccggtatctg cgatagttgt accatttaat tttaaggaac cattgatata agtagtattt
    16981 tcaggaagtg agatcgtgat ttttttgtcg gttatcgcat ccccagattt tgttaacgtg
    17041 aatgtcacat tttcttgaat cgtatctcct gatagagcag acaggggatc tcctgtctca
    17101 ggttgtctgc taaggttttt tagctgacta tctgcactca cagatggaaa attagccact
    17161 gttatttgag gaagagtggt gattccagct tgttcagact gccatttgta cgcaaaagac
    17221 ggatacatgt tagtaagtac atcgtccacc aagcgataat caaaataata ttgccctgaa
    17281 gccaatgcag gtagtgtaga agtagctgta tttacacccc cgttatttga ttggatcgtt
    17341 tggaaaagtt gatctgtcca tcctgtcgct aatttttggg ttcctttcga ataaagcctg
    17401 taatggatcg aacccttttt tagttcagtt gctgaccaat tggccgtgac ttggtacgat
    17461 gcagaagaaa ggagctttcc ccactgacta tcggctgtta agtttgttgg taaaaccgtt
    17521 ccgtctagct tgtactgctc caataccttt aaagcaacag gagaaagcaa tccgccttgc
    17581 gcatctttcc cactgtacac gatattggct gtctgattca atccacttgt gcttgctgga
    17641 gtatgagtca gtattgctcc ttgtgtaacg ttttttcctg taaaatagtt gactacgcga
    17701 tcaaagcgac taaatggcaa ctgatcaaaa cgtccttcag tcagccccat cgtcaatgtc
    17761 cgactattgg tagagcctgg aaggttctgg ttatttccat ccacgtattc tgtggtatca
    17821 gcaaatgtat tatattttcc tgttgaatca cttggtgaga tgtaactatt ccacgtattt
    17881 atctctgcac ttgcagaaaa tgcatatgct cctggattca agtttccgcc agttaattta
    17941 ggataaaatc tacctgcagc tggagctaaa atcgaattga acatacgatc ttggataata
    18001 ttattcgaat cataaaaatc aacagaaaaa acggcctttt tagacataga aggttgtaat
    18061 ttacccttgg tcaaaatttt gtttggactc gtatcatatt tatctccgtt ttctgtcatc
    18121 ttcgcgacat tagctgtaac atcaaattga agcgggatat cgtccacgtt gaattcgcct
    18181 cgcgtaataa tagcaataat atctgtcaat agagtatttg tatcccacga ccccatccca
    18241 aaacgcaagg tatggcttcc tgctgataat aaacttccgc tagtaacctt gatgggggca
    18301 ccggcaatat acaagtaggc gttgttccag tcaattgctt ttaatacatc actgcttgat
    18361 acatgttccg gcaaattgat gatcgccaca aatgcaaatc cttgtgcagt gatattcacc
    18421 aaatcaagta catttgttag tttcacatgg ctttcccaat gaaaacggac cgtgccacct
    18481 ttccaaggat catttgaacc atcgttcaaa taagtagcag tcaatttagg caattggtta
    18541 gccccagtta gttggacaag cttcccgcca ttgactgcac tacgtccacc atttggaggt
    18601 acgatatttt catacgtgta tggatcataa gaaatggcag gaggcgttgc tccaacagct
    18661 tctgccgaag gtgaaaagat aaatatactt aaaacaaaaa gcgaagtaaa aaagaaggag
    18721 cgaaaaaatg aaaatttatt ttgtagcaac atgttcatcc tccttttcct ttcttctcca
    18781 taacaacaac cacgcaagaa gccataattc tataccaatg atcaaataaa gaaaacggat
    18841 cttttgttca ttcgtctccg ggaatacgcc atgttgatta gattctttat aaatcggtag
    18901 ctgtgtctgc tgttttgatg cgccattgac tgaaccactt ggatgcggaa ttggacttag
    18961 atcataaaat gtaatcgtac ctgaactttt ggtatctgct tggactggct cttgccagct
    19021 gaagaacagc aaacttgcaa taatccctcc caataagatc gatgttttca acgaaatcac
    19081 ctcatcatgt tgtttctcct tttttctttt tcttgtggac ccacaaaaac agcaaaatca
    19141 accataacac tagaactgca gcaaccaatc ctgcataaag ccatgggaaa gattgttctt
    19201 gtactgcaaa aacggaattt tcatttactt tttgtgcttt ttcttttttg atcgtaaagt
    19261 tttgtgtaaa tcgccattca ttactgtcgc ctgattttgc tatgatttct gcgcggtaat
    19321 cgccggcttt tgctttatca ccagctaaat caagattcaa gtgaaaattc gaatttggtg
    19381 caactaaaaa tgcattagac gtattttcaa tataaagctg attttcataa aagatcttgc
    19441 tcgtaaatat caaattgctg ataatcctag gctgaggatt ttggaaacga agctgaacag
    19501 ttggctttgc gttgacctcg tccaaatctc ctgataaaag tttcagatcc ggttctatcg
    19561 tctcttgact ttcttgtaaa acgactgcga gattataagc aatacgattt ttgatttgtg
    19621 ctccttctgt agcattttct gtttcagctt cttctttagg tgaaacatga atacccgcta
    19681 atacacgtcc cttaaagctt tttgctggga ttttcacttc tataggtaca gttgtttctg
    19741 catgtgctga tacattgatt tcctttttcg caatgagcaa aacgtcacgg acatcaaacg
    19801 gcaatgtctc atctaacgtt gggttttttt gactataatc gatcgttcca ccatcactcg
    19861 ttaccgccgg attgacagag acctcaaatg ttcgttcttc atctgaagaa ttgctgataa
    19921 ttacttctag tgtctgattt tgttccggtg ccactaataa atcaaaataa ccgattgctt
    19981 tatctatttg gttctctgga aataccggtt tgattccaaa gtctcctgct gaatcctttg
    20041 cttggctgat tgacggaaaa atgaaaaata gacttaaaat agaaaaaaac gaaacaaaca
    20101 gtcgtttact ttgattcatt gtccaccaca tcctttcaac aaaaactgcg gtaaaaaatc
    20161 cttcacctat agtattttca ccgcagtttg ttgaatttat ttcatactct atgctactgg
    20221 gccagccacg aggttccaag tcaaatcagc cgtgaatgtt ccagctgctg ctttcggcac
    20281 tttcagtgtg atatttgctg gatcataaac agaaagccat gttcccagac cttgatcttt
    20341 tgctgctgta aatattggtg tcgctgcact tgctttactc aacccagtta cagtttgact
    20401 agttggtcca gtggattcgt tatctgacgt acttttaaca gtggatgttc caaaatctaa
    20461 ttcagcgcct tgcaaaactt gtgaaccgtt tacaaaatct gaaaccgaaa ctgtcacgtt
    20521 ccacccttgt gcttggccat ctgcacctac acctcgacga tctgatactt ggagataagg
    20581 tgtatcgttt tttgaagcgt ctacttgata cgtttttgtt ccgctttcaa tctcgtgtgt
    20641 accaaatggc aattcgggaa ctacatccag tgttaaagga cctgtctggc cagttgctgg
    20701 attttccgga tctgctggct ctactggttt tgaaggatca gtagggttaa gaggctccac
    20761 ggtatttgtt ggttcagtaa aggtaatatc tccttttgaa gtcgcttgac cctcagcaaa
    20821 agctggtgtt gccaagccgc ctagaagaag tgttgaaagt aataatgtcc caatcatgtt
    20881 ctttttcata ataaatcact cctttttctt agtgacctaa gtatagtaga gacagtatgc
    20941 aaaaaatcct catgaattca tccgcttact tgaaattatg accctttttg tataaaagag
    21001 aaaaaaacaa taaaatcgtt tacatgaata ttcttccttt tttattaagt atgaaaaaat
    21061 atttcaattt tattttttcg tttcaaatta ttctattagt gacatccttg aactataaaa
    21121 ataggagtac ggcatcatct gccgcactcc tatttttttc gtcatttgtt tcttaaataa
    21181 aacgattctc acgcaatttt atttttttga tatcttctac tgtttgtgtt ccagctaatt
    21241 gcatgaccat ttccaattct gttttaaaga aatcaaacac ttgatggact cctgtacttc
    21301 ctcctaaaga caatccataa ataacgggtc tgccaatagc aacaagatct gcacctgatg
    21361 cgattgcttt gaaaacatgc tgtccgcgtc gtacaccgct gtcaaacacg attggtacac
    21421 gtttatctac tgcttctgcc acatattgca acgaatcaaa cgctgctgga ccaccatcta
    21481 gttgacggcc accatgatta gatacccaga ttccgccagc accagattct aatgaacgat
    21541 agacatcttc ttctgactgg acacctttca catagactgg caaatcagaa tgagcagcaa
    21601 taaattctac atcttttgga ctaagcttct gtttggaaga tttgtacaca gcatccatcg
    21661 tttggcctac tcctgattga taagcctgaa cgatcggcat tggcagtggg aaggtaaacc
    21721 catttctgcg gtctgtttca cggtttccgc caactgttgc atccgctgtc aatacgattg
    21781 cttttgctcc attacgcttg gccacttcaa gaatatccaa attgatccca tcatctttgc
    21841 tcatataaaa ttgaaaccat tgcggtgctt tctccccgcc tgcttctctt atctcttcta
    21901 atgtacaaga agcgtaagaa cttgctgtat agatcgtccc aaaatctgct acaccttttg
    21961 cagaagcttt ctccgccttc acatgagcta agccatgagc agcaactggt gccatgatga
    22021 ttggtgcagt cagcgtttct ttgtcaaaat gtgtagttgt atcgggtaat tctacatctt
    22081 ttaatacatg aggaataatc agctgatgat tgaatgcccg ttcattttct tgataagtaa
    22141 ataagtcgcc agcaccactg ctgatgtaac cataaccacc agctggaatc acttgtgaag
    22201 cggctatttc taaatcttcc atattgataa aatccactat tccttcattt gtacctgctt
    22261 gatacgtttt ttccatttca atgccttccc tctataaaaa attgaatgaa taatatgata
    22321 gcgtttacac actttatatt actcttccgt attgatacag gcaaataatc tgtttacatt
    22381 tcagtcattc cagttgttac tactctgaaa gaaatgttta gaacacaaat agataaaaaa
    22441 cgatgggact ttgacaaaag tcgtgtcaaa gtccctttta cgaaatactg tggatgccta
    22501 ttgaaataag tgcgggttgt ttctttgtat ttcaatcgtt tcttgccatt cttcgtttaa
    22561 ttttggatta ccgttttctt ccttgaacgc ataaggtccc aatccaaaaa gctcagttag
    22621 tatttgattc acgattccat atagctcggt gtgatcggac acatgattat tgaagatcat
    22681 ctcattatca ccacgtttga atctcacatt cgggctctca tgatgaacct ctatgcagtt
    22741 ttcccctttt tttaactgac ggatttctga aaacatctca taaatatctt ctccaataaa
    22801 accttcagca agcgccagac tgtccacaag accgcctact gtcaactcag agattatcgc
    22861 tttgttggca ccaagcttta atgtatagct tgttgcttct actacgccgc atgcattgca
    22921 accaccttct gatagttcgc tttttctgat caattgaact tctttcattt tctgttcctc
    22981 ctcttagttt tctacttatg tattttttat cacctgacgt aagtgtattt tatcgtcacg
    23041 atcacgtgat ttattcttca gagattctat ttatctatct aacatatcct ttattcttct
    23101 acttttcttt ctttagcata taagagatac gcatagtaat caagcaagga ttgttgatat
    23161 aaaaagaaca aataccgcta ttttgatttc ttttgactct attactcata taaataaaat
    23221 ttattatacc tgctcttttt gactatatcc tcatctgact cctatacttc gtttgtatta
    23281 aaacttagga ggaatcattc atgcttacag ggctaaactt attaaataat ccttttctaa
    23341 ataaaggaac agcttttaca aaagaagaac gggcaaaata cggaatcact ggtatgcttc
    23401 caagtactgt ccaaacattg gaacaacaat ctgtccaagc ttacggtcaa tacttaagca
    23461 aacagaccga tttggaaaaa cgtatcttct taatgaatct attcaatacg aaccggacat
    23521 tgttttataa attgatgggc gaacatttag ttgaattcat gcctgtcgtt tatgatcctg
    23581 tcgttgcgga ttcaatcgaa caatacaacg aaattttctt gaaaccacaa gatgctgcat
    23641 ttctttcaat cgacgaacca gaaaatatca aagcaagttt gaaaaacgca gcagatggac
    23701 gcgacattcg tttgatcgtt gtgacagatg cagaaggaat cttaggtatg ggtgactggg
    23761 gtgtgaacgg tgttgacatc gctatcggta aattgatggt ctacactgct gcggctggta
    23821 tcaatcctgc acaagtcttg cctgtatcaa tcgacgctgg tactaataac gaagaattat
    23881 tgaataatcc attatactta gggaatcgtc atgctcgtgt agaaggcgaa acttactacg
    23941 aattcattga tcaattcgtt caatcagcta ctgaattatt cccagaattg ttattacatt
    24001 gggaagattt tggacgagga aatgctgcaa cgatcttaga aaaatacgaa gataaaatca
    24061 ctactttcaa tgatgatatc caaggcacag gaatcgttgt actagctggt gtattaggtg
    24121 gattgaatat atcaggtgag caattgaaag atcaaacgat cttgacattt ggtgctggta
    24181 ctgctggtgt aggtatcgca aacatccttt tagatgaaat gatccgtcaa ggtgtacctg
    24241 aagaagaagc acgccaacat ttctatcaag tagataaaca gggtttattg tttgaagata
    24301 caaaaggatt gactcctggt caaatcccat tcgctcgtaa acgctcagaa ttcactaatg
    24361 ctgatgaatt gacaaactta gaagcagttg taaaagcaat ccatccaacg atcatgatcg
    24421 gtacttctac ccaacctgga gcattcactg aagaaatcgt gaaagaaatg gcggctcata
    24481 ccccacgtcc aatcatcatg cctttgtcta atccaacaaa attggcagaa gcaaaagcaa
    24541 aagatttgat tgaatggacg gacggaaaag ctttagttgg aacaggtatc cctgctgctg
    24601 atgtggaata taacggtgta acttaccaaa tcggtcaagc aaataatgcg ttgatgtacc
    24661 caggtttagg gcttggtttg attgcttcaa cagctacacg agtaaatgct gaaatcattt
    24721 ctcaagctag tcgagcatta ggcggaatcg ttgatgtaac aaaaccagga gcagctatct
    24781 tgcctccagt agcaaaaatc actgaattct ctcaaacgat tgctgaaact gtcgcaaaat
    24841 cagttgttgc acaaaaattg aatcgtgaag aaatcacgga tatcaaagaa gctgttgaat
    24901 ctgctaaatg ggttcctgaa tataaatctt tagaagatta agattgttat agtaaaagaa
    24961 catacagaaa ctttgtatga cgtctaaaaa gaaaaaagcg actgcggcaa aacagcagcc
    25021 agccaactac tgctcttttg ccgcttctct ttggcttttt tcatacgata cagaataaga
    25081 aaggatctgt aaaaatggca caaaaaacaa caaccgcaac tgcagttgag gaaaagaaaa
    25141 aagaagggtt ttggtcaaca aaaattaccg gcgtaagttt gcccctttat ttgatcatgg
    25201 tccttgtcct gatcattgca atggctttag gaaaattacc gacgaatatg attggcccaa
    25261 tcgctgtttt ggtcattttc ggtaatttat ttcattttat cggaacaaaa atccctatcg
    25321 tcaaaagtta tttaggtggt ggctccgttt ttgcgatctt tgcctctgcc gcgattgcta
    25381 catttgggat cttacctgag tatgtagtaa aatccacaga aaactttgtc agcaatatgg
    25441 gctttttaga cttttatatt gctgccttga ttactggtag catccttggt atgaaccgta
    25501 atctattgat gaaagcttct gttcgtttta ttcccgttgc acttatttca atggtcgtct
    25561 ccttttttgc cgtaggactc gtaggtatgc ttatcggaaa tggttttgca aattccgttt
    25621 tatatatctc attgcccgca atggctggtg gtgttggagc cggagctgta cctttatcaa
    25681 cgatttatgc aaatgtttta ggtacggacg cttcttctat catttcccgc ctgattcctg
    25741 cgacagcaat gacaaatgtc ctagctatca ttggtgcagc gttagttgct cgtgcgggac
    25801 aatcaatgcc aaaattcaac ggtaatggta aattaatgga aaaaggcgac cttggcgatg
    25861 gaaaacctag aaatgtaaaa ccttccattc cacaattagg tgtcggtttg atggtatcat
    25921 tgactttctt catcttagga caaatctgta actattttgt tcctaaagtc catgcttacg
    25981 cattcatgat cattatcgtt gttatctgta aaatcgcaaa cctattacca gaatattacg
    26041 aagatgcagc tatcatgttc aacaacttga ttgtcaaaaa cttgacagca gctgttcttg
    26101 ccggtatcgg tattgcgtta ttgaatctaa atgtattggc ttctgctttg acatggcaat
    26161 ttgttgtctt atgtttgaca agtgttatcg cgatttcagt agtttcagga tttgtcggtc
    26221 gtttgtttgg tttatatcca atcgaatcaa ctattactgc tggcttatgc aacaactcga
    26281 tgggtggtac aggaaatgta gctgtcttat ctgcagctaa tcggatggaa cttattgctt
    26341 ttgctcaaat gggcaatcgg cttggtggtg ccatcgtttt gatcatctca ggtttcttga
    26401 tgcaattact ttcttagtca aaaaaaatac ctgatcatca agtatcggct tgatgaccag
    26461 gtattttttg ataaaaattt acaggatttt caaaaatctt ttttgcattt tatcggaatg
    26521 ataaccgatg cgttcataaa atcggtgtgc ttctactctg tattctgcag aattaagtct
    26581 tattgctgat attcctcgtc ttttcgcttc ttctacgcca gtcatcaagg cctttccgat
    26641 tcctcttttt tcagcatgtt tagacacagc aagtgccata atattgaaca tcggatcgct
    26701 gaaaatcgat tcataggact ccgcttgtac atagccagct gcttttttag aagtaggatc
    26761 ttcatagacc aagaaaaaat ttttttctgg ctccttcaac agtttcttca tttgtctcac
    26821 cgtaacggtt aatggtacat catatcctaa ttgttccgca ttgatttctc gtagtgcttc
    26881 tgcatctcct aaacgcattt cccgcaacat ccaaatcacc agccaatttt tattttttta
    26941 aatgaatttt tttatcacag tatactcctt ttttcattta gaacaatatg tttcagtgat
    27001 tttgacttgc gttagtctag attctcctaa tgtttgtaag atgacgaaag cattgtaaag
    27061 attgatcaaa agaaccacga ggacaattac ttgaaaaata aaggtcactt tttttggaga
    27121 caaaaccctg ctaaatcttg ctccccacaa acctccaata acagcagcgg ggataacata
    27181 aaacaacata gataaatcaa agcgcataaa gctacttgtt aaagcaatcg ttaccaattt
    27241 tgccagttga gaaaagaaaa tcgtagatag agaatatagt gttgcttctt ttattggcaa
    27301 tgcaaacatc agcatcaaca aagaaacatt gactggtcca ccaccaatac ctaaaaaact
    27361 agctaaaaag cccagaaccc ccccacatat aaggtaccaa gtccatgacg tcaaatgaaa
    27421 ttttggctga tgatgcttgg tgtagaagaa cgcaaaaacc aaggtcaata ccgtcaaaaa
    27481 aatttggatc agctgcacat gcttctcgtt ttcaaataac tgtaaaaaaa cttcgaatac
    27541 aatgttgccg actactcctc caacaacagc tccactagat acccaaccga caatctgcca
    27601 attcagtgat ttcccgcttg atacttgtcg cacagtcgaa acaatggaca tcgtgaatac
    27661 cgcaactgtt gagtaaaaag aaatccctgc aacagaatgg gcgccgataa gatcaagaat
    27721 cggtttgatc aacacgcctc cacccatccc ggaaatagcg ccaactgtgt tcgccagaac
    27781 gatcaccaca aaataaatca gaccaatcat cttgtatttc ctttctcttg tttatatctt
    27841 ttagcgattg ttactctttt cttttattat acagtaaaag aaacaaacga ggcttcgaaa
    27901 gcttttctac tttttaagga aatttagtgc taaacgaaaa gaggttgtga aaaagctgtc
    27961 ctgcatcaag taataagaac agccaaacaa aaatagctcc tcataatttt cgtttggttg
    28021 aacttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggtc gtgagcctgc
    28081 cgttcagctc cgagtcacaa ggaacaattt caaaaaacag cttctcatgt ttgttgaata
    28141 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt taaggaagag actgtgaagt
    28201 caacgttttg acctgattat tagagaaaca aatggcaaaa tagttcttca tatttcgccg
    28261 tttttcgagc gaatgccgag gggcagctga ttgaacatcg tttatttgga aaaagggatt
    28321 gtgacaagac gtttgtcaca atcccttttt ttatatctta ttaattcatt cttttgttct
    28381 attcttcttc ttccgccatg aatgagaaac ccatatcaaa gaactataaa ggttatcaaa
    28441 acagcgttta tgactaaatg aattagagtt atctatcact atttttcaag cgatttaaac
    28501 caaatggcat acaatatggc atacaaaaac ttcaataagc cgtgggggtt tttaaatttc
    28561 ttcttgttct ttaaaatcaa tgagtggagc tacataattt gaatccactt tttcaaacgt
    28621 cactttttgc atcgacgaat gttttctttc ccaagataac gtaaatagat ttgactctgg
    28681 acttataaag tctacttcac cttctagtat tactttctta tgcaaatatt cctctaataa
    28741 atatagactt gctaaagcat acaacacatt ttccaaattt gcttccttat aatttgaaga
    28801 acgaccatgt tttactctgt tatatatttt ccaccaatct ggtgtatctt caggccaccc
    28861 atctccaaaa ggttttagtg aagtcaatgt taaagaattc ataaccctaa caccattatc
    28921 caaacacatt aattcgctag atttaataac agctttaaaa tcagttatgc ttttattgct
    28981 actgctatca aatccgcaaa gttctttgaa aataatctca cactccgaac caatcgctag
    29041 tagtagtttt tgatattcaa tagagaatgt tttgaaatta ttctcatcga gcatcacaaa
    29101 atctgtggta ttaataaact cattttctaa atgtagataa tacttccaat aaaaatttgt
    29161 aaactctgct ttgtccatta aatcacctct tttgaattat aacaaaaaaa acacgtccac
    29221 aacagacgtg ttctttgatt tctacacaat cagcttgcca aaaccatcca atcagtccta
    29281 gcatgcctgt attaaaattg aacgttcatc atctatcatc tttcttagtc cagcatgttt
    29341 gcaaccacac ctacaatttt cagtccaaca atagatttcg actagctaag tcttgcaaaa
    29401 ttcaggcagc taacccttca tttactacct taattatact aaatacccac aacgtttaca
    29461 agtgtaatca ttctaagaaa tctgtttagc tatgatcgtt gtataatctt taaacgccgt
    29521 tccttcattg attgaaataa ttttataact cttgtttttc caagctactt tcatatcgtt
    29581 tgttagttca taatcttgat cgtatcgaat aataaacgtc aacgttcctt caagaatggt
    29641 acctacacta gcaagcacgt cttttagcat ttgtgaacgc acattagccc aacattcaaa
    29701 tacagtttct tcactatcca ccatctgccc atcttcatct tttacccgtt ttgtcttttt
    29761 aaacttgact ctctcagtca attcatttac attcttaatg attggcataa taagcacctc
    29821 tcatttgttg tactaatgct tgtaccgtta ctggtatccg ttcgctgctg gcttcttgac
    29881 ggttcaaata ccaatgttga gtaagtaatg acaccgccaa atcaaattgc tgataattat
    29941 ccatatcttt atccgttgcg ctactatcaa tagcactttt tatatattcg cctgctgtat
    30001 caatcaattg ttgaataaag tcatcatcaa tagtgtgatc gatccgcata ctttttttta
    30061 tgctatccaa aattaccatt ttatctctcc taatgaaaaa aaggcagtgg gataaaccgc
    30121 cgcccaatat ttttctatac ttttggtgtt acaactggcg taaactcaat atacactgaa
    30181 gcattttgat caattttctt ataatcgttg cgaacgatca ctgcaagtcc ttgtgagtag
    30241 taatcgaatt tttcccattg agtcgttact tgatttctac gagcaacaaa gatagattgt
    30301 gcaatatccc ccataatcat agggaatgtt ccagctttag gattggcaaa caatgtatct
    30361 gcaatcaata ctactggcat accgaataat gatttaccac taggtgctgt cacatcgggt
    30421 tgtaaaatgt aacgtccatc tgaatctttc aatgtatcta ggtggtaata tccgctttgg
    30481 tttagaatta ccattttatt taatgctggg tctaatgcca cattgtatag ttgtttcaaa
    30541 tcgtccaacg tagcagccgt tttcttagtg aatgttttta ataaatccat gatatgctta
    30601 ttgtcagtgt tttctaccaa tttcgctaac tgatctttga cctcttgtac aatattcact
    30661 gctgaatctt ccacaacctc atttgataag gcaatcttac cagcacgagt ttctacttta
    30721 taatcaactg aagtaaacat ttcagcgtca atatcaccaa tttcagcaag ttcagctttt
    30781 gttgctaaca ctgcttgttg gttagttgct actggatatt taccttgtcc atttgatact
    30841 gtttttactg ttgcatattg agctaagtta taatttgaac gtttcaaatc aaagacttca
    30901 ccgattactt cttcgggaac aactgccgct gcatttacag tagtgactcc atcacgcact
    30961 tcgccttgtg aacggatata ttcttcatac ccacgcaatt ctgttttttc gttatcgatt
    31021 aaagtttttt ccatgttatt actctccttt tgtgctttag aagtggattc taagctattg
    31081 tttgtttgtt ttgcttggtt gttactcata aacgattcgt aagaacgctt atttacttgg
    31141 acatttgatg aatcataagc gggaacggtc acgacactaa tttcattaag tgctttgatt
    31201 ttatctattg atcgttcaat tgtgccatct tcttttttgt cgaatgaatc taatccaagc
    31261 acgaacccaa aggacataga atcaacaacc ccttttgaga tattctcata cacatcattt
    31321 gaataggtcg tgtcggttag ctctgcttca aaatgtaatc ctgtatcatc gacattcaat
    31381 ttcaacgtac ctgctttgac actagctagt ggctttgaat aatcgtgtcc atataataag
    31441 aacacatttg ataaatccac ctcttttaat gcttcgggtg taatcacttc tacaaagccg
    31501 cctaaatctt ttgacggttg cccgaatttc aacgcatagc ctgaaatagt tttgccgtct
    31561 gttggttgtt tttcattttc tttctctact ttgggagaat cggctgttag ctcagcttct
    31621 tctgtcagcc gtttttcctt ttcttcttca ttattcaact ggtgtaactc cttcctgttc
    31681 ttttaattga tatttctcca acgtatctaa atacgtaaag ttcaaacttg ctagtaaacg
    31741 atcgccacca tccatggggg gtagccccat tttcgaccgc ccctcattaa tcgtgagaag
    31801 tgaccctttg acctgatcca atacattttt tacttttgtt tcaggatcag tctctagtaa
    31861 ctgatcggaa ttaaagcgga tattggtttc aagtttcgta tctaattcac tcacaaacac
    31921 attaaagtaa tgaatcagcg tactttggat atactggaga ttactttgaa cggtgctaga
    31981 atgttcattt tcaacgccta aacgctcaat cggtacacca aacgccttgg caatttgttt
    32041 tgtgttccaa tcattggaat tgataagctt caaaacatcg gtgtttattt ctaatgtttt
    32101 gtagtccatt gtttcatcaa gaatgatcgt tctaagagca ttatctccac tacttgaacc
    32161 gttggcttct tcaaactttt cacgaattgc gttctttgct gatccgtcta aatctgactt
    32221 atgaaccttc aaaatacctg acccactgac accacgagta aagaaattgt gcaatgtgcg
    32281 gttgccagct tgttgaattt tcagttcatc acgtaacgca taaagtggtg gtaatcctgt
    32341 taaaccatct tgggtgaaat atttgaagtg caaaatatcg ctagacttca cacgtctttt
    32401 tttatcacca atctgataag acaacgtacc attatctaat tgagtaaccg ttacacttga
    32461 gttaggtaat aagtgaattt cttctacctt ttcaccgttg cgtttaatct ctgcaaagct
    32521 attaccgttt agcaacatat tgaccgccaa agcaaacttt aaatgccaac catccatttc
    32581 tgaattaggt ttctcgttta gtaacttcac cagttcatta tcagcttgtg gcatattgtt
    32641 tttgaccaat tgaatcggac ttgaagcaat atcactagca ataattcgaa cggctgcaaa
    32701 cacatcacta tttcttaatg cacgaacgct tgtaaagctg gtggtgtaat catccgattg
    32761 gattgatacc acatgttgta ggaaaggatc tcccgttgta ttttgtctcg ctttaaaaaa
    32821 tgccatcttt taacctccct tctgttctgt atttagcatg accgctaccc caataagcac
    32881 tatgcccagc gctaacaagc catagtagac atttgttagt aaggtgatag caaccaacac
    32941 acacaccaat ccaagaagta aaagaacggt atgaatgtaa ttagaaagaa aattcttcac
    33001 ttgtataaaa ttcattgtct gctttcgctc cttccgttgt ggtgaagtaa tccattgcaa
    33061 acacataggc attgattaat gcagctattg gatcaatttt gttactgttc tttgatttat
    33121 taatctgaat gccgttgtta tccgttttta aaatagcgtt gttcactgca tgggtgagaa
    33181 tcgtattttt attgtgaata acgttgcctt cataaacttg ttctctaaaa gttcgggtcg
    33241 gaacgtttag agtaatcgtt ccttgtctta cttctaacat tgggtagttg gctttttcag
    33301 ctttactgat aagcgaatta gcgttatacg ggtcataaca aatccccata cattccaaat
    33361 cattttcttg aatcaaatct tgaataaact gaaatacttg gtcatagtcc acgattccac
    33421 tttctaattg agtaatggaa cactcaccag ctttttctag ttctcgataa ggcaaaccgt
    33481 cgcgcttttc tttgtcttga agcccatatt tggtggctac aaagctatgt gaatcacaat
    33541 aaagttcgcc gttatctagc ggtacgatcc atgaaacgct agttaaatca tctgttttag
    33601 ataaatccac tccgatataa acgggcttgc ctgtaatatc ttgtggttct acttcggttg
    33661 cctgccagtc ttcagcaatc atatagctgt cctcactcgc ttgtctccat aaattgaagt
    33721 ttttcaccaa tacagaatta aggttatttt gtttcaaagc aaggttcaca tcatcttgaa
    33781 tggttggaat cattactttt ttgatttctt cactttcaaa aatcggattg gctttgatcc
    33841 atttctcttg atcgtgaatt tcttcgggat cgtccaattc ccatatcgct ataaagtacc
    33901 tgtcggcttg ttcttcgcct tttagcacac gatctaacaa taagtattcc tcgtacatag
    33961 gaacgtttaa atcaagccca gcggtactaa taacacacaa taagccgttc ttttgttggg
    34021 tcattcctga cttaatgacg ttgtacgttt tacgagtttt tgcttcgtgc cactcgtcaa
    34081 gaattgcggt tgttggtgca aacccatcta gcgtgctggt ttcactagcc agtgccatag
    34141 cgaacgaatt agagggcaag tgctgaatct tagagttcat aattttcagt tgtggtctta
    34201 aaaacttact agacttgacc acgtttctaa gtgaattaga cagcatatca tagcccaatt
    34261 tagcttgttt caaagcgtta cttacaaaca atacttgcct tgcttctgct ggttcttgtt
    34321 ccataatcaa cgcattggca gccatgccac ttgcgatata cgttttccca ttcttacggc
    34381 tcatactggt aaacgctcga ttgaatcgcc tgtaattccc ttctttagta cgccagccat
    34441 aaagactgcc tagaatgaat ttttgaaatc ctaacatctt gattgcttta ccgtccgtag
    34501 atggtagcaa ctcaatgaat ttaatcgctt tggtggcttg ttcttcatcg aacacataag
    34561 ggaagctatc tgacttagaa cgctccaaat cgcttatatg acgctcacac gcttggataa
    34621 ttttcttggg tgcaatcagc ttacctgata ggactttctc aatgtaatca ttcatgaatt
    34681 catcaactct ttgaacgggt cttcgggttc atcattttta accttgttca aagctagttt
    34741 tgatcgtgat tctaaggtca tacctagtga ggtggcttgt tttttcaaat cactcatagc
    34801 ttgtgattga acccgaaccg ctggattggc tttctttact ccgttttcgc atgtgataaa
    34861 cgtacctgat tttcttatct cggcttcggc tgttttcatt cgagcaaagg ctaaacaata
    34921 ggctaccaat gaaccataat ctgcttcact caatggataa tcttttttta atgttggtac
    34981 gatacgattc cattcacttc tagcgctcgc tgccatccag tcagggggcg tgatatttat
    35041 taatggttgc tgcttgaaca attcttctct agcgtccaca cggattgctt tttcttcact
    35101 tgatatattg ccttttgtat cgtgtaatat tttagggtgt cccatgttgt cctcctttca
    35161 gacaaaagaa aaaggaacgt aagcaattaa gctcacattc cttcgttttt cgatcagaat
    35221 tt
//
LOCUS       DACCXM010000013        34961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCXM010000013 DACCXM010000000
VERSION     DACCXM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.013"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            157..1863
                     /locus_tag="IVY61_000564"
     CDS             157..1863
                     /locus_tag="IVY61_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP7969703.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2021..2923)
                     /locus_tag="IVY61_000565"
     CDS             complement(2021..2923)
                     /locus_tag="IVY61_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7969704.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2920..3624)
                     /locus_tag="IVY61_000566"
     CDS             complement(2920..3624)
                     /locus_tag="IVY61_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP7969705.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3655..4296)
                     /locus_tag="IVY61_000567"
     CDS             complement(3655..4296)
                     /locus_tag="IVY61_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7969706.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4334..4648)
                     /locus_tag="IVY61_000568"
     CDS             complement(4334..4648)
                     /locus_tag="IVY61_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969707.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4636..5265)
                     /locus_tag="IVY61_000569"
     CDS             complement(4636..5265)
                     /locus_tag="IVY61_000569"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP7969708.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="IVY61_000570"
     CDS             complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="IVY61_000570"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP7969709.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5985..6917)
                     /locus_tag="IVY61_000571"
     CDS             complement(5985..6917)
                     /locus_tag="IVY61_000571"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP7969710.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6914..7690)
                     /locus_tag="IVY61_000572"
     CDS             complement(6914..7690)
                     /locus_tag="IVY61_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP7969711.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7744..10929)
                     /gene="carB"
                     /locus_tag="IVY61_000573"
     CDS             complement(7744..10929)
                     /gene="carB"
                     /locus_tag="IVY61_000573"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP7969712.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10934..12010)
                     /gene="carA"
                     /locus_tag="IVY61_000574"
     CDS             complement(10934..12010)
                     /gene="carA"
                     /locus_tag="IVY61_000574"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP7969713.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12015..13295)
                     /locus_tag="IVY61_000575"
     CDS             complement(12015..13295)
                     /locus_tag="IVY61_000575"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP7969714.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13314..14240)
                     /locus_tag="IVY61_000576"
     CDS             complement(13314..14240)
                     /locus_tag="IVY61_000576"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP7969715.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14253..15536)
                     /locus_tag="IVY61_000577"
     CDS             complement(14253..15536)
                     /locus_tag="IVY61_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP7969716.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="IVY61_000578"
     CDS             complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="IVY61_000578"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP7969717.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16324..17235)
                     /locus_tag="IVY61_000579"
     CDS             complement(16324..17235)
                     /locus_tag="IVY61_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7969718.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17232..17696)
                     /locus_tag="IVY61_000580"
     CDS             complement(17232..17696)
                     /locus_tag="IVY61_000580"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP7969719.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17701..18399)
                     /locus_tag="IVY61_000581"
     CDS             complement(17701..18399)
                     /locus_tag="IVY61_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7969720.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18386..18772)
                     /locus_tag="IVY61_000582"
     CDS             complement(18386..18772)
                     /locus_tag="IVY61_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969721.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18919..20589)
                     /locus_tag="IVY61_000583"
     CDS             complement(18919..20589)
                     /locus_tag="IVY61_000583"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP7969722.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20772..21005
                     /locus_tag="IVY61_000584"
     CDS             20772..21005
                     /locus_tag="IVY61_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP7969723.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21104..21535)
                     /locus_tag="IVY61_000585"
     CDS             complement(21104..21535)
                     /locus_tag="IVY61_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP7969724.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21614..22027
                     /locus_tag="IVY61_000586"
     CDS             21614..22027
                     /locus_tag="IVY61_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP7969725.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22065..23213)
                     /locus_tag="IVY61_000587"
     CDS             complement(22065..23213)
                     /locus_tag="IVY61_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7969726.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23335..23931)
                     /locus_tag="IVY61_000588"
     CDS             complement(23335..23931)
                     /locus_tag="IVY61_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7969727.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="IVY61_000589"
     CDS             complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="IVY61_000589"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP7969728.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27161..27592
                     /locus_tag="IVY61_000590"
     CDS             27161..27592
                     /locus_tag="IVY61_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7969729.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27637..28986)
                     /locus_tag="IVY61_000591"
     CDS             complement(27637..28986)
                     /locus_tag="IVY61_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7969730.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29056..30219)
                     /locus_tag="IVY61_000592"
     CDS             complement(29056..30219)
                     /locus_tag="IVY61_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969731.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30348..30671
                     /locus_tag="IVY61_000593"
     CDS             30348..30671
                     /locus_tag="IVY61_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969732.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30854..32203)
                     /locus_tag="IVY61_000594"
     CDS             complement(30854..32203)
                     /locus_tag="IVY61_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7969733.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32272..33225)
                     /locus_tag="IVY61_000595"
     CDS             complement(32272..33225)
                     /locus_tag="IVY61_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP7969734.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33327..34517)
                     /locus_tag="IVY61_000596"
     CDS             complement(33327..34517)
                     /locus_tag="IVY61_000596"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP7969735.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9951 a         7641 c         6120 g        11249 t
ORIGIN      
        1 tacctaaaaa tttggatttc aacgatagaa acactgtttt ttatgtaatt agtccaagat
       61 ttttctagac ttttgcttta ctattcggcc aaattggttt ttcttctttt tttcgttata
      121 atgaatatac ttatgaaaag aaatgaggaa tgaaccatgt cctttgatgg tgtatttacc
      181 cacgcgatgg tcaacgaatt gcgtgaaacg ttactatctg gccggatctc gaaaatccat
      241 cagccttatg aaaatgaaat cgtgctggtg attcgttcac gcggcaaaaa ccatcgatta
      301 ttattatctg cgcacccaag ttatgctcgt gtacagatta cccaaatcga ttatcagaat
      361 cctgataatc cgcctaactt cgtgatgatg cttcgaaaat atttggatgg cgctatttta
      421 gaagacatcg aacaaatcga aaatgatcga gtgatccatt tccactttgc taaacgaaac
      481 gagttgggtg atctacaaaa tatcatattg atcgttgaat taatgggacg tcatagtacg
      541 atcgtcttag ttaatcgaga aactggaaaa atactggatg cgatcaaaca cattggaagc
      601 tctcaaaaca cttatcgttc tttgcttcct ggtgtagaat atgttgctcc gccaaaacaa
      661 gaggtcttga acccatttag ctccgagaaa gaaaaaatct ttcagcggct ttcccaaact
      721 gaactagatc caaaagcaat ccaacgtcag tttcagggaa tcggtttcga tatcgctcaa
      781 gagctgacca aaagattact ggaacgtcct aatgaaaaga tggttgtatg ggacgaattt
      841 ttctcagcca tcagccatca gcctattcct actttttacg aaacggaaaa taaagatttt
      901 ttcacaccga ttgcttatca ggtattgtcc gaacaggcat ctgctgtaac agcttatcca
      961 acactcagtc aattattaga tagctattat cacgaaaaag cagagaaaga tcgggcaaaa
     1021 caacaaggcg gagaactgat tcgaaaaatc gaaaatgaac taaaacgaaa taaaaataaa
     1081 ttgaaaaaac gtgaacaaac actcaaagaa tccgaaaacg ctgagaatta ccgtagaaat
     1141 ggcgaactac ttactacttt tctgacacaa gtaccacgcg gcgcaaaaga agttgtttta
     1201 cctaattatt atgaagaaga tcggccaatc aaaatagcac tagatccggc attgacacca
     1261 aatcaaaatg cacagaaata tttccatcga taccaaaagc tgaagaacgc tgttaagctg
     1321 attggcgaac aaatccaaga agccaaagat gaaatccaat atcttgaatc tgtcttatct
     1381 caactggaaa ttgccggccc aatggatatc gaagcaatta aagaagaatt gacttcagaa
     1441 ggatacctga aaaagaagaa cttaaaaaaa caaaaacgta agaaaccatc tcagcctgat
     1501 caatactttt cttctgacgg aacgctcatt cttgttggta aaaacaatct tcaaaatgac
     1561 cagctgacaa tgaagacagc taaaaaaacc gactattggc ttcatgcaaa aaatataccc
     1621 ggttcgcacg tcatcattaa aagtgacaag ccctcagatg aaacaattac agaagctgcc
     1681 gagttagctg cctatttttc aaaataccga tactctgccc aagtgccggt tgatcttgta
     1741 caggtaaagc atatccgaaa accaaatggc gccaagcctg gttatgttat ttatgaaaac
     1801 caaaagacga ttatcgtgac accagaagaa gaaaaaatta cagctatgaa acaaaacagg
     1861 taatcaatga gtgtttctag aatcatattt gcgtactttc ccacaacagc aattagttat
     1921 aaattgtttg ctacaaaaag aggagttgtg acaaaattct gtcacaaccc ctcttttttg
     1981 atcaacggtg ttcaataaac agcgttctca tctgcttttc ttagctctgc tggtccagta
     2041 accgagtaag ctctttgata aatctttgag cgatttcact gagcggacgt ttcgccgaat
     2101 aaatgatata aaattcattt agttcgcctt ctgccaaagg gattaaccgc aagtgctcgc
     2161 gaatccctcc aatcacaata ccagatcctg aagcgtaagc atcagtttca agaacgatat
     2221 tcatcaaagt acctcgatca ctcgtataaa tcactgtttc ttgatcaggg atctcgataa
     2281 ggtcttcata aaaatacggg aaattatttc catcctgtgt aaaacgaact tgaggataag
     2341 ataacaattc ttctttggtg atctctgcgt gttcagctaa tggatgtcct tgccttaaaa
     2401 aaatatgcgt tttgaatttt ccaatgctgc ggccacttag ctctccttgt tgtaaatatc
     2461 ttctgatacc agcttgatta tgctcattca tataaataat gccgatctca ctgtgctgct
     2521 ctttgacttc ttcgatcact ctcgctgttg tcgtctccat gatccgaaat tgtttgatct
     2581 cgtctttgaa ttcctcgagt agctgtgcgg tcaaaggacc taagaaatca taatgctggg
     2641 cagcgatcga aaaattgtct ctcttttcac tcgttagata gtgagtttct aatgtatcaa
     2701 gctggaccaa tattttctcg gcacgattta agaagtcttg cccttcttct gttaaatatg
     2761 cccctcgatt cgttcgagta aacagcgtga tccctaactc ggtttctaat tctttgatac
     2821 cagctgaaag acttggctgg gagacaaaca ttttctttgc cgcttctctg aaactaccat
     2881 tgtttgctac agcgaccacg tatttcatct gctgaatatt cattgctcaa actcctcgaa
     2941 ataataaatt tctcttccat tcaagggctg gattggtcgg ttgttcggtc gagcatactg
     3001 accatcgaag gggagaagaa actcttggtt cagttttcct acatgatcca ttacaaacca
     3061 ttggattggc ccgctggtag gcggagttgt caatgagcca atataatgga aatggctttt
     3121 ttgctccgga aagaacacat ccggatttac ccaatgctca tgtttttcta agttccaact
     3181 ttccccattt tcttgattat acagccaacc ttcctgtacc attagctggt acatgacacc
     3241 cacaactaga ttctcttttt tgctgttcat atgaacaaaa tgaaattcaa tatcttcctg
     3301 ttttccgttg atgatatgtt cagaaggcaa gtggaaatga atatctgtca aataatagcg
     3361 ttctttcttg aactctacat aactgagttg gtcaaaaggt actaaatgaa tcgtgttttt
     3421 gaattccttg tcagtaaacc gttgcttttg ataatgaaag gaaagcgtgt ctccttctat
     3481 tttggtttct tcccccttgg tcaaatgaat aggcgactgc aatggaaatt ccgcacctct
     3541 cgcaaaccaa tcacataaac tcgcccaatt ttccggacct ttgtctccag aatagcccca
     3601 ttcgacatcc atattctttt tcaactatac ttccccctgc atctcatatc atttttattt
     3661 ttcttctttc tttacaatcg ttgagacggt ttctaaaaac gcctcttttc cataaaattc
     3721 ctggttcaat agttccggaa atatctcttt aaagaaatac tgaacagaag gccgatgttc
     3781 aaagctttta atcgtagtca ctgcctcaat gaacatctcc ttataaactt tttccgggtt
     3841 atttttgatg atttcatcaa aagattcata aagaaggtca accttatctg aaatggctaa
     3901 tagttctcct tctacggttt catcttttcc ttcgaacaac cgacggcgat aaacttcttg
     3961 gaactcagct ggaatctctt ggttgatgaa ttcgtccgtc atcttttctt caactgtttg
     4021 cagcatccct cttaactctt tgcttgcgta tttcaccggc gttttgatat caccaataaa
     4081 acgctctgtg tagtcatgat tcaatgattt ttcatagagc agtttccaat cgatcggttg
     4141 tccattcgct tcttcaatgt ctcctagaac ttgcgcaata gatgctacac gatacgaatg
     4201 tgctgcaact gtatgctctg tatatttaaa aaatccaggt gcccttgtga tcgtctctaa
     4261 attgttcaat cctaaaataa attcattcaa ccccataaaa gttacctccc tttgagattt
     4321 ctttttccga atatcatatc attaaataac tgatattcgc aacatttccg ctctttacca
     4381 tttttgaaac agctccctta ccaaaaaata gagaaaatct atatataata gaaaaaagag
     4441 aagagattat tctcttcttt ctgctaacca gtcttagaaa aatgaatttt aaaaaaatca
     4501 aaaactgatc acgatagaag tttcttttca tacctgatga aaagatgctt ctcgcaatca
     4561 gtttttggat ttttgctatt ccaattgatg attctggaga cacaaatcgg atttctctat
     4621 cttcatcaag cgcgcttaaa tctgccaatt ttcaggatct tttttccatt cttttagcag
     4681 agttaattct tgctcatcga tataacgatc ttctaaagct gcttcaatca atacagaata
     4741 attggtcaac gtcataagag ggatttcggc tttttcgaaa tttgcttttc cttttggcaa
     4801 ctcatacgta aagatagccg caacaccaag gatatccgca ccttctcttt ttgctgcttc
     4861 agctgcttcc aagacacttc cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg
     4921 accttcaaat atacgtcctt cgatttggtt acctttccca tgatctttcg ccttacttcg
     4981 aatatagacc attggcaaat ccagtatttc cgctacccat gcggcatgag ggattcctgc
     5041 tgttgcagtt ccagcaatca cttgtacttc agggtaagct tctttgattt tgcttgctaa
     5101 tccttttgcg atctcttttc ggacttttgg atagctcatc gtgattcggt tatcacagta
     5161 aattgggctt ttgattccac ttgcccatgt aaaaggatta cttggattta aaaatactgc
     5221 ttcaatttct aataagtctt ttgcgatact ttgttcaaca ttcattttag gttagctcca
     5281 ttctgtttga atcgtttgat atgctttata aggattagtt gactgagtga ttggtcgacc
     5341 tataacgata aaagttgagc cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg
     5401 atctcctgcg actgttcctt ctggtcttat acccggagtt aagcaaacaa attcgtctcc
     5461 tgccatttct ttgatcgctt ctacttccca agcagaagaa accacaccat ctaatcctgc
     5521 ttttttggca cagctagcat aatggatcac acttttttct aaagaaactt cgattagttg
     5581 atcggcatgc atttcttctt cacttgtaga agtaagctga gtgatagcta gaagtagcgg
     5641 acgttttcca ccttctggag tcccctcttc taatcctcgc attgccgcct ccatcatacg
     5701 aataccgcca gcggcatgaa cacaggtgag atcaacacca agctttgcca atccccgcat
     5761 tgctttttcg accgtattcg gaatatcgtg aagtttcaga tctaaaaata cattatgacc
     5821 agcctctttt agataacgta cgatttcagg tccttcttgg tagaacaatt ccattccgat
     5881 tttcacaaac aatttttctt ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct
     5941 agttgaaaaa tctaatgcaa taattggtcg ttggtctagt tgattcatcg ttcacgttcc
     6001 tctctcactt ctttgattaa tctttccagc gattcgatgc ctagttcgct cattcgcaca
     6061 gggagctctt taattagttt aggacagata tacggatcgg tgaaattcgc ggtccctata
     6121 gctacagcac ttgctccagc cataaacatc tcaagaacat catccactgt ctgcacgccg
     6181 cccatcccaa tgattggaag gctggatata cttgctactt ggtgaatcaa acgaatagcg
     6241 acaggcttga ttgctggtcc ggagagtcca cctgtttgat tggctaaaat cggttttctt
     6301 gtcttaaggt caatccgcat accaagaaga gtattgatca tcgtaaaccc atctgcccct
     6361 ccagcttcga ttgcctgagc gattggaacg atgtctgtta catttgggga caatttgaca
     6421 tagacaggaa cagaagaaac tttctttact gcttgtgtta attgaaaagc aacatcagaa
     6481 tctgttccaa aagcaattcc gccatgtttg acatttgggc aagaaatgtt cagctcgatt
     6541 gctttcacat ttggtgcttc gccaattttg ctacaaactt ctacatagtc ttcttcacaa
     6601 gcaccagcaa catttgcgat gatgggtaac tcattgtact cctctaattc tggaagaagt
     6661 ttatgcataa caacgtctag accaggattt tgcaaaccaa ttgcattcaa catgccagat
     6721 ggtgtttccg ccactcttgg cgtgggattg ccaaagcggg catttggagt cgttgccttg
     6781 accatgatcg atccaagctg attcaaatca tagtattttc cgtattcttt gccaaaaccg
     6841 aaacagccgc ttgccggcat gataggattt tttaattcta ggccaggaag tttgaccgct
     6901 aaaggattct cattcatata attacctctc ctattttaaa aatcggtcct tcgtcacaaa
     6961 ctttcacact ttttgttcct gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag
     7021 cccctatccc acatgccatg cgggattcta atgataactg gacattttga tgcgaagaaa
     7081 acagctgttc cactgtcttc aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt
     7141 ccgtcttcgt tcctaacaat aaattaccga catttccgtg cataccgtat gttccatcgt
     7201 cagtagcgat tcttgtttct cctagacgat taaattcttt ttcataataa acgacttcat
     7261 gtgtggcaaa acctaaaaaa tgcgttggct taattccttt ttcgactaac tgttttgata
     7321 actcatatag aggagggaca ccgattcctc caccgattat aaatacttcg tctcctgtat
     7381 gaagattgga caaatcaaaa ccattgccta aaggacccat gacatctaaa tggtctccgg
     7441 cagaaagagt agagaagact ctagtcccct caccttccac ccgataaatg atacggcaag
     7501 tatttgtttc gtggtcgata tgattcaagc taatcggtcg gcgtaatagc aagtctgcac
     7561 gcggcacgag aatatgcaag aactgtcctg gttgctgcat ttcttttact agatcacctt
     7621 tcaatgtcat ttcaaaaatc tttggtgcca gctgtttttg ctgaacgatt tccatcattt
     7681 cttgtttcat ctgtcccctc ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg
     7741 ccgttaaata gggttagttg aaaaagcacg ggattccatc actcgaacga tcgcatctgc
     7801 tgtgtctaaa gaagtaaaca atggaatacc atgttcaact gcttctcttc ggatgatgaa
     7861 accgtcttta gaagcatttc cccgatcttt atcaatcgta ttgacaacca cttgtgtttt
     7921 accatctctt attaagtcga ctacattttt ttctgctgct tcctcagaaa ttttcgctac
     7981 tgtttcaacg accagaccat ttttttcgaa ataagcagct gtatgtttcg ttcctatcag
     8041 actatagcca atttctgtaa aacgtttagc taattctagt gcttcttctt ttgtttcgtc
     8101 cgcaatcgta aataatacgg ctccatattc aggcaagtgt aatccagaag cttcgaatgc
     8161 cttgtacaaa gctttgtcta attgtctgtc agatcccatt acttctccag tcgatttcat
     8221 ttcaggccct aagtaagtat caactttctg caatttcgta aaagaaaaga caggtgcttt
     8281 aacatgaact tcttttgttt ctggataaag tccgttttca taaccaagtg tctttagttt
     8341 ctctcccaaa atagctttcg tcgcaatttg tgccattgga attcctgtca ccttacttaa
     8401 gaatggaaca gtccgacttg ctcgaggatt gacttcgatc acataaacct cattttcatg
     8461 gatgacaaac tggatattca tcatcccgac acaattcaaa cctaatgcta agcggatcgt
     8521 gtaatcttcg atcgttttct tgatcgattc agataatgtt tgaggtggat acacagccat
     8581 tgaatcccct gaatggacac cagcacgctc aatatgctcc atgatccctg ggatgagtac
     8641 tgtttcaccg tcacaaatcg catccacttc gcattcacca ccaagcaagt aacggtccac
     8701 tagtactgga tgttctggag atgctttcac agcatgttcc atgtaatctt ctaaatctcg
     8761 ctggttttca acgatttcca tcgcacgtcc gcctaataca tagcttggac gaacaagaac
     8821 tggataacca attctcgaag cgataaccac ggcttcttct ttacttgtag cagtatcccc
     8881 taaaggttga ggaatatcca actctttcag cgcttgctcg aataaatcac gattttccgc
     8941 acgatccaag tcttcgatcg ttgtccctaa gatttttaca ccagcagcag tcaaaggttc
     9001 ggctaagttg attgctgtct gaccaccaaa ttgaacgatt actcctatcg gctgttctag
     9061 ctcgatcaca ttcatcacat cttcgaaggt caatggctca aaatataatt tatccgaaat
     9121 agaaaagtca gtcgaaactg tttcaggatt gctgttcatg atgattgctt cgtatcctgc
     9181 ctgctggatt gctttgacag aatgcacagt cgcatagtca aattcaaccc cttgtccgat
     9241 acggatagga ccagatccca gtactagaat tgagggcttt tctgatcgtg aactttcatt
     9301 ttcatattca taggaactat aaaaataagg tgtttttgat tcgaattccg ctgcacaagt
     9361 gtcaaccatc ttatatacag gaatcaaatc attttctttt ctcatctggc gaacggcttc
     9421 ttcgtttatt tcccacagct ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt
     9481 tttcaatatc ttgtcatcca acggctgctg tttcaactgc tgttcgattt ctacgatatg
     9541 caacagttta tctaaaaaga ataaatcgat ttttgtcaaa tccgctaatt cttctatggt
     9601 gtaccctctt ctaatagctt cagctacata aaacaggcga tcatcttgtg ctcgaaccac
     9661 tttttggatt aggtcatcgt ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt
     9721 accgatttct aaagaacgca cagctttcaa taaagactct tcgatattcc gaccgatagc
     9781 catcacttca ccagttgctt tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt
     9841 atcaaacggc cagcgaggaa ttttagctac aacataatct aatgcaggtt caaattctgc
     9901 aaaagtcgtt cctgtaacag gatttttcat ttcgtctaat gttaatccaa gcgctatttt
     9961 agcagctagc ttagcgatcg gatagccagt cgccttactt gctaacgcag atgaacgaga
    10021 aacacgcgga ttgacttcaa tcacataata attaaaacta tgtggatcaa gagcgagttg
    10081 gacattacag cctccctcga tcttaagtgc tcgaataatc gaaagagaag catcacgaag
    10141 catttgatat tcatggtcag acaatgtttg actaggggca aatacgatag aatcacctgt
    10201 atggataccc accgggtcaa aattttccat attgcagaca acgattgcat tatcagccga
    10261 atctctcatc acttcatatt caatttcttt aaaacctgca atacttcgtt caatcaaaca
    10321 ttgggtagcg ggggaaagac ttaatccatt ttctgatacg atacgtaatt cttcttcatt
    10381 ttcacacata cctccaccag ttccgcctaa agtaaaggct ggacgaacga tgacaggata
    10441 gccgatttta tttgcaaaat ctacagcttc ttctactgta ttgacgattt cagactcggg
    10501 gattggctga ttcaattctt tcatcaactg tttgaataga tcacgatctt ctgcttgatc
    10561 gatcgcagaa agctttgttc ctagaagttc tatgttcaat tcttctaaaa tacctgatgc
    10621 tgccagctcc attgccatat tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc
    10681 tggacgctct tttcgaagaa tccttgatac aaattcaagt gtgatcggtt cgatatatac
    10741 ttgatctgcc acttctttat ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac
    10801 ttcatatcct tcttctttta aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc
    10861 ggcttgacca atgatgatcg gaccagatcc aatcaccata attttcttga tatctgtacg
    10921 tttcggcatc tgtttactgc tcctttcctg cgtccatcat ttccataaat tcatcaaata
    10981 aatgtagtgc atcatgcgga ccaggggcag catctggatg aaactgcaca ctgaaagcgg
    11041 gataatctct gtgtttgact ccttctactg ttccgtcatt tacttctaca tgagttacga
    11101 tgagtttttc tggatcaatc gtggcttcat cgactgcata gccatgattt tgagaagtaa
    11161 aatcaattct tccggtcgct acttctcgta ccgggtgatt taatcctcga tgtccgaatt
    11221 tcattttata ggtatctgca ccgttggcta aagcaaataa ctggtgaccg agacaaatac
    11281 caaaaatcgg tacttttcct tggatttttt tgatcatttc gattgcttct ggcacatctt
    11341 ttggatcacc aggtccattc gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct
    11401 cggcatctgt gttataggga agtactgtga ggttacagtt tcttttactc aattctcgta
    11461 aaatactatg tttcaagccg aagtcaatga caatgacttt ccgacctgtt cctggacttg
    11521 gataaggttt gttagttgag acttgctgca cttgattggt tgctagaacg gttgctttca
    11581 gctgatcaaa tgcatgttcg aaggaatctc cagcatcaac tatactagct ttcatcgttc
    11641 cgacttcacg gattttgcga gtaagggcgc gcgtatcgat acctgatatt ccaggaattc
    11701 ctttttgttt caaaaatgaa tctagcgtca tttggtttcg ccagtttgaa gctacacgtg
    11761 catgttcttt tactactact cctttgcatg ttggagaaat cgattcataa tcatctctat
    11821 ttaccccata attacccacc ataggatagg taaatgtaat gatttgaccg ttaaaacttt
    11881 gatcagtgat agtttcttga taaccagtca tacttgttgt aaaaacgatt tctccgaaga
    11941 cactagcatc tgcaccaaaa gcctttcctt cgaaaactgt tccatcttct aatataagca
    12001 aacgttccat acttttattc tcccttctgc cacacaagtt ttccgttgac aaaagtcatt
    12061 tgtgtttcgc cttttacttt ccagccgata aaaggtgtat tctccccttt tgataaaaag
    12121 tcttctttat cgatcgtgca agtttgtgta atatcaaaaa ctgctacatc agcgggggca
    12181 ccaaccgtta gcgttcctgc attcaaacca aaaatctcag ccggtttaac tgccaaccaa
    12241 ttgatcactt gttccaacgt gaatcgacca gtttctacaa aatgagtata gatcagttga
    12301 aacgctgttt cgcttcctac gataccaaat ggtgatttca tgaaactttg gcttttttct
    12361 tctaatccat ggggggcatg gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct
    12421 tcgatcaaag cttctctgtc ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg
    12481 aaatcttcgg gaatatcttc gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt
    12541 acgtggattc ctgctttttt tgcgtctctg attacacgga cactttcttt agtagaaaca
    12601 tggcaaacat ggtaatgaac accagttgct tcagctagca acagatctct agcaatttga
    12661 gaagactcgg ttacgcttaa gatcccggga agaccaagtt cttctgcctt cttgccagcg
    12721 tgcataacgc cgccaaataa taaagattca tcttctgtat gggctactag ggctttattg
    12781 ttttttgcag cttctttcat agccagatac atcgtcccag ctgtttggac acctacaccg
    12841 tcatttgtga aagcaaaagc accttcttca atcagtgctt cttgatcaac taatttctca
    12901 cttcgcagat tttctgtgat tggcgcatac tgcaatacct tcacaactgc atcttttcta
    12961 atgatgtcat atacttgtct cagcttttct gccgtatcag gtacaggatt taaattcggc
    13021 atcgcgcata cagtagtgaa accgccacga gcagctgagc gagtcccagc ttcgatcgtc
    13081 tccttgtatg taaatcctgg ctctctcaaa tgaacatgga cgtccactaa accaggtgtg
    13141 atcaattgtc cctttgcatc aaacacttca tcaaattcgg cttctgagaa tcctgtaccg
    13201 attgctttga ttcgcccatc ttctatccaa atttctgctg gtgtcgtcat attttttcgt
    13261 gtattgatct gaccgttttt aatgagtgtt ttcatttttt catcctccta taattacgct
    13321 tttccattta gcactgcttc taagattgcc attctgacga atactccatt gctcatttgc
    13381 tgaacgatcc gcgactgtaa tccttccact aatgaatcag ctaattctac atcacggtta
    13441 actggtgctg ggtgcataat gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt
    13501 aacccatgtt cctgatggta gccttctttt gagaaacttt cttttccatc atggcgttcg
    13561 tgttgcaccc gaagcatcat catgacatca acttgatcta gtagttcatc caatggcata
    13621 taatgtccgt acacttcgaa ttcatcgtcg taccattctc taggacctga gaagaatact
    13681 tgtgcgccta gacgttttaa catctgcata ttggatttag ctacgcgaga gtgagtgata
    13741 tcacctacga tggcaacttt cagaccttca aaatgaccaa attcttcata aatggtcatc
    13801 aaatcaagca agcattgggt tggatgctgt ccgcttccgt ctcctccatt gataatcgaa
    13861 cactggattg ttttactttg tatcagttcg tcataatagt tctcatcacc atgacggata
    13921 accgctacgt ctacgcctaa tgctgacatc gtcagtactg tatcgtatag tgtttcccct
    13981 ttttggacag agcttgtgct tgcttcaaat tcaatcactt ctagccctag ttttttctcc
    14041 gctacatcga agcttttatg tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag
    14101 tactggcgct tttgcggagt ccaattagca ccttgcttga attcctgccc tctgcggatc
    14161 aagcccataa cttcttgatc agtaagtgcc tcaacggtca ataaatgctt taagctgata
    14221 cgttctgatt tgatgatcat aatgtttcct ccttagtttt cttcgcttcg tgaagtttct
    14281 ggtaagataa gatttaaaat gatgccaagt acagttgcta atgccattgc cgacaatgta
    14341 aatgtgccta cttcaaagac gagtccgcca attcctacta ctaagataac tgaagcaatc
    14401 aacagatttt tctttttatc aaaatcgatt ttgttctcga ttaaaatctt cattccactt
    14461 gcggcaatta ctccaaataa gataaaactg attcctgaga tgactggtcc agggatactt
    14521 aaaatcaaag cgcttaattt tcctacgaat cctaaaatga ctgcaaatac tgcagctcca
    14581 ccgatcacaa acacgctatg gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa
    14641 cttgttacag gaggacctcc gacaaaggct gccacgattt gtgctaatcc atcacctgtc
    14701 aacgtcttat gaagacccgg ttcttcaaag aaattacgtt tagttagttt attcaatacc
    14761 ataagatggc cgatatgctc tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt
    14821 gctccaagat atagctttgg ttcatattgt acaaagggaa tctcaaagtt aggaacagca
    14881 aaccaggcag cgtctacgat tggcttagta tccacgattc caaacagcaa ggctacgatg
    14941 tatccgctaa taatacctag taaaatcgga atcaatccga gaaaacctct tagccacatg
    15001 ttgaagaaaa ttgttaatcc taatgtgatc agcgctacag caatatactt gaaatcatac
    15061 gtgccctgat tatacatagc atttgttgct gcatttgccg caagtcccaa cccgataacc
    15121 atgatgactg gaccgacgac gattggaggc aagattttat ccagccatgc tgaaccgatc
    15181 tttttgatga tcagcgcaac aatcaaatag actaatccag ttgtgactgc tccttgtgca
    15241 atcgctggat aaccatctgt tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg
    15301 cttcctaagt acgcaggtat ctttccttta gtagtaaata agtacaccaa tgtacctagt
    15361 cctgaactga ctagcgcaat acctggatcg atacctacta agatggggac taacacggtt
    15421 gctccgaaca tcgtgaacaa gtgttgtaaa cttaatccta cccaatgtcc tggggttggg
    15481 cgatcgtgga tatctaacac cacgtcatca ttatgaaatc cttttttctc cgacatgttt
    15541 ctctctcctc taatcttcct ctttcaaaat catgatgcga tcttgtccat caagctcttg
    15601 catctctact aaaatctctt ccgcccgaga tgttggaatg ttcttcccaa cataatctgc
    15661 acggattggc aattctcgat gcccacgatc gaccaggact gctaatgaaa tttttcttgg
    15721 tctgccaaaa tccattactg cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac
    15781 atcatcaatc aaaataactt cttttccttc taaggaaact gggatgtcag aagaatgaag
    15841 ttccggttct tctggatttt cttttttgtc atctcggtat aaagtgatat ccagttctcc
    15901 tacaggaata tcgatatctt ctagctgctt caggcgttcg gcaattcttg atgcaatgta
    15961 gattcctcgt gttttgatac ctaccaatac gatatcttgg atactatggt ttctctcgat
    16021 aatctcataa gtaattcgag tcaatgctcg cttcatggtt acctgatcta ctacttcttt
    16081 tgcttgcatt ttcattcccc ctactttttt gcataaaaaa actcctgccc taagaggaca
    16141 ggaggaatgt ttagactgag cgtatactga tacactgata gctatactat gtcaatgagc
    16201 aatctactta tggcgcacca tacgtatcct tgctcccacc cttcttagcc tcacgggact
    16261 aaacttaaag gaattctatt aagttttatg ctttcgttgt taattactat accgttatct
    16321 aaattaaaat gcaatgtttt ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag
    16381 aggtgcttca aagatcatct tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc
    16441 atggagaaac tgaccattgc ctttcaaagt tttcttcggt ccgtatactg gatctccagc
    16501 taacggataa ccgatatact tcatatgcac acggatctgg tgtgttcttc cggtttctag
    16561 ttttaattca accagagtaa atccttcgaa acgttccaat acagtaaaat gtgtgacagc
    16621 cgacttccca tcttctctta tcgcctgcat tttccggttt tccttcgaac ggccgattgg
    16681 cgcattgatg gttccttttt cgtgaggaat ctctccatgg actaaagcaa catatttgcg
    16741 caaagaggtc ttatctttta attgctgagc taatgattcg tgtgcctggt catttttggc
    16801 aaccattagc agtccggaag tatctttgtc gattcggtga acaatacctg gacgaataac
    16861 gccgttgatc gtagaaagat ccttgatatg gtacatcagc gcattgacca ttgtgccatg
    16921 cgtatgacct gctgaaggat gaacgaccat tccttgaggt ttgtttatca ccgctacatc
    16981 ttcatcttcg taaacgatcg tcaacgggat attttctggg accacatcta atgtttctgg
    17041 ttcggggatc aagacagtaa tctcgtctcc ttgttttact ttgtaatttg ctttgactgg
    17101 ctgtccattg acagaaaccg ctccattttt cagccacagc tgaatttgcg aacgactgta
    17161 atcggtgaat aaattagtca atactttatc gatcctacca gattcatggt gaatcgtcgt
    17221 tttaagttct gtcatttttt tctttgatcc ttttcatcta aaataatata aattaacata
    17281 caaatcactc cacatacaag tgtcacatct gctacgttaa aaatcggaaa gctcataaaa
    17341 tctgtctgga acatatctac tacatagcca agacgaatac ggtcgataaa attgccaatc
    17401 gcaccagcta gaatcaaact caatccaatc atgaaccagc gattaccttt ttcacgattt
    17461 ttaatcatca atgtgagaat cacaacgact gcaatgagtg tcactatagt aaagaaccac
    17521 atcttccctt ctagcatact ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa
    17581 atacctggta taagattttt cgtctctcca agcgctagat tatcagtgat ccaccatttg
    17641 accaactgat ccactcctac tagcaaagcg ctgaatatcc aataaataat taacaaaaaa
    17701 tcaatccttt tctatttgtt tataaatatc acgaggtcgt acgatcccga gcagattacc
    17761 atcgggattc ccatctttag tgataagtac ggcttccaaa gtcccttgct cacgaaacat
    17821 cgcttcaacc tcgtaaatat gtgttttagc tggaacaaaa tgataatttg tgtactttcc
    17881 gttttggatg atcaaatcca acgctcgttt atttgttaaa tcaaccggac ctttttggct
    17941 ttcttttgct agccaaaacc cgatattacg taaagtaatc aatgctacaa attttccttt
    18001 attgtataac ggaaattgtg aatacctttt ttctgctact atctttaaca gttcaagtaa
    18061 cggaagatct tgttcaaaac ctgttacacg ttttgcaaat cgaggcagca cggtttcagg
    18121 ctgaatcagt tcttgttcga tcagctggat tcgcttgact gtccaatcat ttggctctgc
    18181 aatgacaaaa tcgactgcga tccgatcatg gacgatcgca ttccttaact gagcaagctg
    18241 taaaagatca tcttcgtatt ttctgatcat taattgtttt ctttgagcta ggcgtctgac
    18301 aagttcggta aatcccatat ttcgtgcatt tcccatctcg tctctcatcc atttttcgat
    18361 tcgattgaaa ctgattaaaa aaagatcaga gtttgccata attttcctcc ttgatcaaat
    18421 atgcccggtt ttttatatag aaaaagaacg caatcaacac aataactgca aaaaaactga
    18481 aaaataaatt ttgtaggaaa acggccccca acccagttgc tagaaataaa aacggataga
    18541 tttgaagaat caagtaattc attgtgatca cataagcagg ctttgcttct ttcactgtat
    18601 aaacggggct ttttagccaa taaaaggtga cctgcatcgt aaagggcact tccagatcaa
    18661 gcgttatctt ttgttcatga ctgagaacac tgcgtttttc attgttgatt cttagctgaa
    18721 tcgagctacc cataccgtaa aaaccggttt ttcttataaa tgtcgttttc atcataccct
    18781 cctattatgc tttagtatag ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa
    18841 caaaattaat tccctatcgt ctaaaaaaag aagctgtagt caaaatacag ctccttcgat
    18901 ggtagattat aatcaagctt aaaacaatcc caacacttct ccgtcatttg tcacgtccat
    18961 attcaatgca gcaggttttt taggcaaacc aggcatcgtc ataacatctc cagtcaaggc
    19021 tactaaaaat cctgccccta attttgggat gatttcgcgg atcgtgatgg tgaaatcttc
    19081 cggtgcacct agcagtgaag gatcatcaga aaatgaatac tgtgtttttg ccatacaaat
    19141 cggcagtttg tcccatccat tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct
    19201 gaaaacaaca ccatttcctc cgtaaatttt cttgacgatt gtttctgtct tttcttgtat
    19261 cgtttcttct aggcggtata atggcttgta attagcttct tttctatcaa tcaatcgtac
    19321 tacagctttt gctaaatcaa cgccgccttc tgctccatct gcccatacac tcgctctctt
    19381 ggcaagaatc ccctgatttt cacacaagtg ctccaacagt gtgagttcat tgtctgtatc
    19441 tgttacgaat tcattgattg cgacgatcac aggtagctga tattgttcca tattacgtat
    19501 atggcgcttt aaatttgcaa aacctacttt caaagcatca agattctcat ttttaagttc
    19561 atcttttttc attccgccat gcattttcaa tgcacggaca gtggcgacaa tcaccacagc
    19621 atccggcgct tttttcaagt taggtacttt gatatctaaa aatttctctc cgccaagatc
    19681 tgcaccaaat cctgcctctg tgacaacgta atctgctaat ctcaatgctg tcttggtagc
    19741 taaaatacta ttgcagccat gagcgatatt ggcaaacggt cccccgtgga caaaagctgg
    19801 tgttccgtaa attgtttgta caagattcgg cttgattgca tctttcagca gtaatgccag
    19861 agcgccttct gctttcaagt ctccagcagt cacaggctga cggtcaaatg tatagccgac
    19921 aacgatcctt gcaagacgag cctttaaatc ttcaaggtct gccgcaagac acaagatagc
    19981 catgatttcg ctggcaactg tgatatcaaa cccatcttct cttggaacac cttggattgg
    20041 cccacccaag ccaacaatta ctttacgcag ttcacgatca ttgagatcga ctacccgttt
    20101 ccaaatcact cggcgggcat cgatgttcaa ttcatttcct tgatgaatat gattatctaa
    20161 aagagcagat aatgcattat tagctgttgt gatagcatgc atgtcaccag taaaatgcaa
    20221 gttgatatct tccataggca atacctgtgc atatcctccg cctgcagcac cacctttgat
    20281 tcccatcaca ggaccaagtg agggttctcg caatgcaata acagattttt tattgatttt
    20341 attgagcgcg tctcctaacc ctacagtgat cgttgacttt ccttcgccag ctggtgttgg
    20401 attgatggaa gtaactaaga tcaaatgccc ctcagcgtta tcctttgatc gattgattcc
    20461 agaaaaattg attttcgcct tatatttccc atacagttcc aaatcgtctt ccttaagatc
    20521 taatttacta gccacttcaa caatcggttc tagctctgtt tcttgtgaaa tttgcagatc
    20581 attcttcatt tgattacccc ttttcacgaa ctttattcaa caaaatatct tctttgttaa
    20641 gatatgccaa ttataccttt ttttattcag aaaaaaaaca taaaattaca ggtttttaat
    20701 ttatttgacg tacttataaa tactctttat aatgaaggct atgatcagac tttagaaagg
    20761 tcgtgagtgc tttgaccaca ggaattgtaa aatggtttga caacaaaaaa ggatacggct
    20821 ttatcagtta tgatgacaca gaagaaattt ttgtacattt tactgcgatt gaagaagagg
    20881 gattcaaaac tttggaagaa aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag
    20941 gaacacaagc tgcccatgtc aagaaaggca acgttcagac taccgatact agtatgcatg
    21001 aatagataaa caaatagagt gagacgctaa acgggatgaa ccgtacgtct cactcttttc
    21061 ttttcctgta aaaattcgtt tttttaggtt ctgattttta aaattagctt tttctttttt
    21121 tcgtataagg ataaggaatt gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa
    21181 ggactacttg atcttttttt cgcatcatga aaacagatgg ggctttctca tcttgcaaca
    21241 tcatttgcac cccagtatta gttggcgtca aagatcgaat ctgtccgaca gatatttctc
    21301 ggtctttcca aaaagcagca taacgtataa tcacagagtt tgattttaaa ataaaagaac
    21361 gccgcaaacc tagaaataga aaaacaagaa aaatacctaa aacaaaattg cttttccaat
    21421 aaggacctgt gttttctaaa gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg
    21481 accaatagat gatcgtatgt gccagttcag gctgccatcg aatatttttt ttcattctct
    21541 aacctctcac cgattcagta atgatataat cttaccataa cattagtttg aacaaaaagg
    21601 agaatctctt tttatgttaa aagtatacat tgatgcctct acaaaaggca accctggtcc
    21661 tagtggaggt ggaatcttga ttgtctatca aggtaaacaa gaacagctat ctatcccgct
    21721 ttctataggg acaaaccatc aagcagagtt cgaagtcttt ttaaaaactt tagaatttct
    21781 aaagaaaaac gacttacaga atgaaacgat tttctgtttt tcagacagca aaacactagt
    21841 ttctacggtc gacaaaaacg caacgaagaa cgagctcttc cttccctacc ttcaaagaat
    21901 ccaagaactt ctatccgaat tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa
    21961 taaaggagca gataatcttg cacgtcaggc tctccataaa caattaaaag aaaaaaagac
    22021 tctttaaatt aaaaacttta aggagtcttt ttatagtttc tcatttaata taaaagataa
    22081 ttgtgatcaa acgtaatttc caattggtaa tctccgattt tttcttctat ttgactacgt
    22141 aagattcttt ctagttctgt atcaacaaac tggaatttct cagggaccac tacatcaaat
    22201 tgcagcgttt ttccttgatg aattactcta aaatcatgaa tattaagccc tgtacctgtt
    22261 tcaagaagaa tttgctttaa ctgccgcaag atattatagt atttttcgtt atcaacaggc
    22321 attggatcca agtgacagac aagttccaca tccaaagact ttttaaagtc tttttctatc
    22381 agatcaataa tcttatgagc cacattcaaa ttcattgatt catctacttc gatgtggact
    22441 gatgcaaagc gtttattggg tccatagtta tgcaccaata aatcatgata gccaagtata
    22501 tcatcgcaag catttaaatg ctcttccatt tcttcaatct ctgctggcgt tgggcgactt
    22561 cctaatagtt cgtatacaaa atttcgaagc atcgtcaatc cactataaag gatatataat
    22621 gctaaagcaa aacctatata tccatcaata cgccatccag ttacccattc gattgctgct
    22681 gaaagcaata cagccagtgt agtaaagaca tcattataac tatctgttgc tgtagctctc
    22741 aatgtttcag aatcaatgga ttgcgcaata acctgataca ttttgccttg tgcaaatttc
    22801 aaaaagatgg aaatgattag gacaaaaaag acgatgggtg ttaaagagac attttctgga
    22861 tggatgattt tttcagcaga agactctaaa aattgaaatc ctacataagt aatgatgatc
    22921 gaaacaaata acccactgat atattcgaag cgttcgtgtc cataaggatg ttcctgatct
    22981 gctggctttg cagcaatttt aaatccgatc aaagtcaata tggatgaagc agtatctgat
    23041 agactgttca ctgcatcagc cataatggag acacttcctg aaaaaagccc gatcattagc
    23101 ttagagacaa acaagatcag attcgtaacc aatcctaaga tacccgctag tactcctttt
    23161 tttgtccgct gttgcggtat ttgtttcttt ttttcttctt gtttcttagc caagtttttt
    23221 accccttcta gtttgcttgt tgctcaggta aaaagatgga gaggacgatt gttttcctct
    23281 cctctcttcc tttttctatc tatcgtgcgt cctattacta ttaaaagatg gtcattattc
    23341 tggtttcaac ggtgttgctg caaataacgc ttttgctagt aatggtgtta tgattcccgc
    23401 acctattgct tctggtatcc cattgattgc tgcaacccct gctaaggttt taaataaaag
    23461 agcagagtct acaccatagg catctgccgt tgcacctgta taaaataatc ccatgaatcc
    23521 taacactaag actgtattga ccaatgaacc cagtacccct gcaaaggctg agctgatcgt
    23581 tattgattta tttttttgat aaatccagtg gaaaaccaag cctgctacta agccaacaag
    23641 tactctggga actaccgaga taatcggatt cgtgaacact aatgtatcaa atggcgttgt
    23701 tggcatggca taggcacgaa caattgtagc gattccccaa aacaagccaa tcaccatgcc
    23761 atcttttggg cctaatacta ctgcagcaat aattactgtg atatggataa ttgttaaact
    23821 aatgaatcct aatggaataa accccagcca aggaaccatc gcttgtacga tgatgattgc
    23881 taacaagatc gcacgtaaga ctagtcgaaa ggtcttattt ctactattca ttttttcacc
    23941 cctttttatt tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg
    24001 aatccgacca tttgattaga tgcgccatcc aattgaaaaa agtatgaggg caagacttgt
    24061 ttgtcttcgc ccccataatc atgtttcgtt caatgtttga acttttgttt ttctgtcaaa
    24121 cgtaaacgcc ctattgttgc gcaacccatt ctttcgcagc tttaagtgca tctggaatac
    24181 cagcaggatt tttcccgcca gcttgtgcca tgtctggacg tccaccaccg ccaccgccta
    24241 ctttcggtgc gattgcttta atcaagtcgc cagcttttag tccagcttca tttgattctt
    24301 tcgtcattgc cgctagcaga ctgacttttc cttcttgttc gtttgctaaa acaagaatat
    24361 ctgaaatatt tttttgtttc cattggtcag ctaattgacg cagctgattc atatctttca
    24421 cttttacttg tgctgcaatg attgtttttc cattgatcgt ttctggatct ttgaatacat
    24481 caccagcttg ttgattcgct aacttgctag ctaattgttc attttccttt tgtagctctc
    24541 ttacttgctg ttgcaattgt tccgcttttg aaacaacttc tttcaattga ggtgatttaa
    24601 caattgttgc tacttgtttt agttgttttt cttcttcttc aagtagttcg tacgcttctt
    24661 tacttgttac agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt
    24721 tgaagattcc gatgttttct gtatttgcta catgtgtgcc tccgcacagt tcgattgacc
    24781 agccgccaat atttactact cgaacgtcat gtccgtattt ttcaccaaac aaagccattg
    24841 cacccatgtt tttggctgta tcgatatctg tttcaattgt ttcaactggg atagcttccc
    24901 agattttttc gttgacgatt ctttccattt cagctaattc atctgaagtg atttgaccaa
    24961 agtgggtaaa gtcgaatcgt aaatgtccag gagcgactaa agaaccagct tgatttgcat
    25021 gctcgccaag tacttctttc aatgccttgt gcagtagatg ggttgctgta tggtttttga
    25081 tgatccgatt gcgcatcgat tgatcgatca atagatgata ggtttgtcct tcactcattg
    25141 gtgcttttac ttctacggtg tgcataaatt gaccattagg cgctttttga acatctgtaa
    25201 caactgctac gatttcgcct acttcattca tgatccatcc ttgatccgct acttgcccac
    25261 ccatttctgc atagaaaggt gtttgatcaa agatcatttg agcttcacct tctgcgactt
    25321 cattcaaaag agactcacct ttaacgatga ccaataattg actatctgat tcttgttctg
    25381 tgtatccaat gaatttactt tctactttga tatctgtcaa taaagcagat tgtaccccca
    25441 ttgatttttc agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt
    25501 caaaaccagc atgatcaact ttcagtcctt catcttctgc cacctcttcc gttaattcta
    25561 ccgggaagcc atatgtgtcg tatagtttga agatgtcttt tcctgctaga gtcgtcccgt
    25621 tttcgttttt tacttcttgg atcacttgat ttaagatttc caaaccttca ttgattgttt
    25681 cattgaaacg ttcttcttct gtacggataa ctttttcaat aaattctttt tgttgaagta
    25741 cttcaggata ataacttacc atgatttccc caacaacagg gactaaacga tataagaatg
    25801 cttgttcaat acctaatttt ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga
    25861 cataccctct cccttcattc gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc
    25921 gaatatgatc tgcgattact ttgtaagaaa tatctgttac tgggttttca ccatatttta
    25981 cttgtttgct tagcttttct acggcgtgga taataggcat aaacaagtct gtctcaaagt
    26041 tcgttgacgc atcttgaacg attgagacca tgcgttccaa tcccatcccc gtatcaatgt
    26101 ttttatgagg aagtggttca aatgtgttat caggcttatg gttgaattct gaaaatacta
    26161 agttccagat ttctaaataa cgttcatttt ctcctcctgg atagttttca ggatcgtctt
    26221 ctgctaaatc gttataagct tctccacggt catagaaaat ttccgtatcc ggaccacatg
    26281 ggcctgcacc gatatcccag aagttgtctt ctacatcaat aatgtgatct agtggcaggc
    26341 caacttcttc atgccagatg cgttttgctt ctgtatcttt tggataaact gttacataaa
    26401 gtttttctgg atcgaatgcc atccattttg gatcagtcaa aaattcccat gcccagtgga
    26461 tggcttcatt tttgaaatag tcaccaatcg aaaaattacc cagcatctca aacatcgtat
    26521 ggtgccgtgc tgttttaccg acattttcga tatcatttgt tcgaatagat ttttgggcat
    26581 ttgtgattct tggattttct ggcacaactg aaccatcaaa atattttttc aatgttgcaa
    26641 caccagagtt gatccacaat aaggttggat cgttcacggg gatcaaagac gcactaggtt
    26701 caatcgtatg tcctttagat tggaagaaat caagaaacat ttgtcgaact tgtgcactac
    26761 ttaattgttt catgtcatta cctgctttct ataaattaaa tacaaaaaaa caccatcaca
    26821 gccaaggacg aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc attacctctt
    26881 aaactcgata acgctgagct gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt
    26941 gattagtgac tattcttttt tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa
    27001 tcatatcctt ttcgtactta attagtatac gcattctatt gatcactgtc aatatctcaa
    27061 tttttatttt catttccttt tggtcatact ccacgatttt tggtacgata agaataaagg
    27121 aatcaataca tctctgctat tcatgacaag gaggaatatg atgaaaaaaa tagttggagc
    27181 ggatgaatgg atacgcgcag ctagctgctt tttgagatat caagtttttg tattagaaca
    27241 agggattcct gcagaactag aattcgacgg aaaagatatc ccgcaacaaa cctattatct
    27301 atttcttgat caaaaaagac cagtagctac tttgcgttat gaaaagaaaa cacctacttg
    27361 cttcaagccg gaacgatttt gcgtagcaaa agaataccga ggaaaagggt taggcggtca
    27421 gttgcttgaa gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta gttatgtagt
    27481 agctgaaatg acggctgtag atttttatga aaaacaaggg tatcatactt gcacagaacc
    27541 ttttattgaa gatgaagtac tttgtatcgg aatgaaaaaa agtctagctt aaaaacaaga
    27601 gattgtgaca cgccttttgt cacaacctct tgttttttat ttatgtgaat ttttcttatt
    27661 tttacgagca gtacggcttt gctgtctgcg ttcgattttg cgttcttttt tatttttttc
    27721 tgcaattgcc caatcgattt ttttcttata accaggcttg atctttttct ttttcttctt
    27781 cacaagacca atcagcgtgg gatctaatac atcttttgat ttttctcgtt tggcacgtct
    27841 attacgatcg tatgttttga cgatttcccc gtttttgatt tcttttggtt caaatgagac
    27901 gcccaatttt tctatcgcat caatggcttg ctcatcattt ggcgaatata acgtaatggc
    27961 cgttcctttt aagccattac gtccagtacg tccgacacga tggataaaga agtctagatc
    28021 ttctggaatt tctgcattaa taacatgaga gacaccttcg atatcgatcc ctctagcagc
    28081 taaatcagtc gctacaacat attgatactc cagattctgt acttgtcgca tcactcgttt
    28141 tctctctctt ggggtgatgt ctccatgaat ctttgctact ttcaagcctt ggtttttcaa
    28201 gtaatcggca atttcatcca catgctgttt tgtattggca aatacgattg ctaaataagg
    28261 atgaccaatt gtcagcagtt gataaatcat ttgattctca tctttacctt ttgttgaaat
    28321 cagccaattt tcaatgtctt cagaaataac tgcttttggt ttgatttctt caatgattgg
    28381 attttccatg tattttcgta agaatggacg aagtttttct ggaatagtgg cagaaaaaac
    28441 taaaaactgc agcttttctg gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc
    28501 catatccaat gtcatatccg cttcatcgac aacaaatgca aatgcggtgt gaatcttcag
    28561 cgcttgttca ttcatcatgt ctaatattct accaggcgtg cctactacaa cctgtggctg
    28621 ttggtgatgc agacgattta attgacgctg tttgtctgta ccgccgacaa aattcgagac
    28681 acggatctcc ggtgtagaaa atttagcgat ttgtgtagct gcttcataga tctgtgcagc
    28741 tagttcccta cttggagctg taataacaac ttgtacttcc tctttttctg cattgatttt
    28801 ttcaaacaaa ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag attgcccaat
    28861 gatatttttt ccttttgcaa ttaaaggaat caaacgttcc tggacctctg tgggctgttt
    28921 gaagcccttt gcggttaacg cttcattgat aaacggtttg aagcggaata tatcaaaatt
    28981 attcattcgt gcacctcttc tctttaaatt atcacgactt tggcattata acacagacaa
    29041 agcgtttttg ctcagctaat ttcgagaatc atcatccttt gtttggccaa tcgtgctaac
    29101 agtatatgtg tgactagctt tttcataaaa cagaatccgt ttttttctca aaaatgatag
    29161 caagatattt aaaataaaaa taccttgtag taacaagaca ccgagagaaa tgaatactgc
    29221 actcatctgc aacaccgtat tcgttgtcga attcatgatt tgctgaagca actgccccaa
    29281 ccagcttaaa ccataaaaaa gaaagtacag atacccgtag cggatcatca atgcttttaa
    29341 agaaattcgt tcgcggtctt cttccaccaa acggatacgc acaatttttt tgccaagtgt
    29401 ccatccattg gttataaacg gtacaatcat aaagtatacc cctacttcta ataaaagcca
    29461 ccagatatga gaagtaactg cccctgaatc gaacgcaatt ttaaagaaaa tcgccgctac
    29521 caaattaata agagataaaa atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa
    29581 tgagacagtc tttccttttt cataagaaag ttcatccatt cgtcttcttg taggaagcat
    29641 aaatgtaaat aacggcgtaa cgacaaaacc aatgattcca cccaatgtat ttgtcactaa
    29701 atcattcaca tcaaataagc ggtatggtct tgggtagata aaataaagtc cacttagctg
    29761 cgtcaattca aaaaatagag aaaggcagaa actagcaatc atcgttttca taaaagaaag
    29821 ccggaaataa tatcttaaat aaatgccaaa cggtatgagt aataaaacat taaaagctgg
    29881 ttccaaaaat acactctgct tcattgccgg caggtaggta ctaggttcag acaagaccaa
    29941 taccgtgttg tctctgaaat gttgccatga agcaaataaa tgcagctcca tctttggccc
    30001 tgtcatttgg gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa aatacgcaca
    30061 caataaataa aaaacgaaag aatacaaaat aactgccctt gttactagaa atgttccata
    30121 ctttcgatat tcataaataa aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac
    30181 aatcagcgca aaacgaatcg gttcgatata acttgccata tgactcctcc ttgattcttt
    30241 gtatctctct tatcttagca gtcctttgct gtttcaagca agtaacgagg ctattatttc
    30301 tcatttcttt caaagtaaag taagataaac acaaaggggg tgtcgagatg ctgaatatgt
    30361 actttgtttt tggcgtacct atttttttac tgttccttta tgctacgatc gcttatgtaa
    30421 gaaaaagaac tacaattcat tatttaggat ttattctcct gattatttcg ggtttcatgc
    30481 tagtcttcaa tcttcagact tggcagcaag cacttcttga aatggataaa atgactccac
    30541 atgctttgtc taaagtgctt ggctaccctg tctatctgat ttggcttccc attttcattt
    30601 ctggttgctt ggttttatta aatatttatc gcggcgtacg ccgaatagtt cagttacgca
    30661 aatcaaagta aaagacagaa atcggttgag cccgatttct gtcttttact ttgctatttc
    30721 atcgtagaca aaacgacatt tacgtgatca aaattaacga gtacttttga catttcttcc
    30781 acgtactcct catttaaata aaatattttg tttggcagag aagtgcagtt ctaataaaat
    30841 tcgaatgttt tagctatcta cgttttaaat agagataaat cgggatacca gcaagtgtca
    30901 atactatccc catcgaagct aaaaaggtct gagtgataag cgtgcttgat acgataaaag
    30961 tccctccaat gattgctact agtggaataa acggatacat tggaactttg tatggtcgaa
    31021 acaaatcagg ttctttttta cgaagaagaa tgacaccaac aaagaccatc gtataaaaaa
    31081 tccaaatgac aaagatcagc atatccgtta gtgtatcaaa accaccgatc atcatcatgc
    31141 caatagcgat gactagctct aagatacctg cggctacagg aactttagtt ttatcatgaa
    31201 gtttagcaaa gagcttgcta aaaggcaggt tgttttcttt tgccataaca taagggagac
    31261 gcattccagt aagggtatag ccattgatgg ttccataaac agaaatcaag ataccaactg
    31321 tgaccagtct tccaccgaag ccgccaaaaa tcatttttgc aacgtcactt gccgcattgc
    31381 tgtttcctgc gactccgtcg atagatgcgg ttcttaagaa caccgcattt actaataaat
    31441 aaacgatcat gattccgatg attcctaaag aaattgcttt tggcaaatct ttcgccggct
    31501 ttttcaattc tcccgaaata tttcctacgt gtatccaacc gtcgtatgcg aacatcgttg
    31561 ctagcaagcc cgctcccaag gctgagaaaa atgaaagatt ttctcctgct tggatcggaa
    31621 acagttggaa gtctactcct tcttgtcgga acaaaccgaa aatgacgatc acaaacaaag
    31681 gaataagctt gcagacaagc gtaatcgact gaaaagcccc tccagcttta gaacctaaaa
    31741 aattgatcaa caggatcgat acagcagctg taacagctac gggaacaatc attgactggg
    31801 aaagaccaaa tagattgacg aactgtgtac cgaaaatgat cgacaaggct gctacatttg
    31861 ctggaaaata aatcacgact tgcgcccagc ccaataaaaa agctgctgta tttccgtaaa
    31921 tccgttcgat atatttaatc atccctcctg tttctggaat agcagctgcc agttcagctc
    31981 ctgttagtcc tgcacagaca gaaatcatac caccgagaaa ccaagaaaac atatgcaaac
    32041 tggcggatcc tgtaacttct gctacgcttg cggctttaaa aaatacccct gcaccgatca
    32101 ctgtgcccat aacagtcgat aaagcagtaa aaaatcccat cgtacgtttt aattcatttt
    32161 ctatttttac ttcttttact ggcgtggctt gatattcttc catcccttgt cccctttcat
    32221 accatagcca atcccaaaag tataaaaaac tttttggatg tggttcttgt tttataccgt
    32281 ctctaccaat ttttctttca gtttttccaa catatccttt gtcatcacat ctaagtcgta
    32341 tttcggcgaa aagccccatt cctcttgcgc acaagaaaca tctagtgaat ttggccaaga
    32401 ttctgctatt gactgacgta caggatccac atcataatcc atttcaaaat ctggtatgat
    32461 tttttgaata gatgctttga tctcttccgg gtcaaaagac atcgccgata tattgaaggc
    32521 atttcgatga accaatcgat caggatctgc attcatgagt tttataatgg catcgatcgc
    32581 atctggcata tacatcatat ccattgatgt accttgatca atgaaactag tgtactttcc
    32641 ttctttgatt gctgaataat aaatatcgac tgcgtagtct gtcgtaccgc ctcctggcaa
    32701 ggttgcataa gaaatcaatc cagggaaacg gacaccacga gtatctacgc catatttcac
    32761 ataataataa tcacatagta gttctccagc taccttcgtt accccataca tggttgtagg
    32821 acgctgaaga gtatcttgtg gggtatgatc ttttggagta tttggaccaa acgcgccgat
    32881 tgaacttggc gtaaaaaatt tcagctggaa ttctctagcg acttccaatg cattgaccag
    32941 cccagtcacg ttcaaattcc aggctaattg tggttttgct tctgcaacag ctgacaaaag
    33001 agcagccaaa tgaatcaatg tgtccacttg atattgttct gctatgctgc gcattttttc
    33061 gtaatccatt acatctagga tctcaaagat atcgtttttc tcaatgactt caggcatgcg
    33121 tatatctgtc gcaatgacat tttcactgcc tagttcttga cgtagccgtg tgatcaactc
    33181 tgaaccaatt tgtcctaaac atcctgtcac caatactttt ttcatgatga tcctccctga
    33241 tcttttttac tactatcttc agatgattaa caatatatgc gtgaacatgt tctctaattt
    33301 taaatagacg aaaaaactaa atttagttaa ataatttcca tttcttttcc tattttttga
    33361 taaactgcta ctgcttcatc aagcatttcc ctcgtatgtt ccgcagtcgg catatttctg
    33421 atcctgcctg tcccaagagg cacagttgga taaacgattg gtttagcata aatcccatgt
    33481 tcgatcagct tcttagagaa agtctgagtc actttttcgt ctcccaaaat gactggagta
    33541 ataggtgttt cagacatacc gatgttatag cctacttttt tcaattcttc tttaaaataa
    33601 ttagcatttt cccaaacttt ttcgacaagt tccggatgtt cttgcatcaa agtaatcgaa
    33661 gccaatgcag ctgcagctgc acctggagtt agcgaagtag aaaataggaa tggccgactt
    33721 ctagctttca accagtcgat tagtgttttc gatccagcta cataaccccc aacaacacct
    33781 atagctttcg ataacgtgcc catttgcata tcgattttgt cgcttaatcc aaaatgcttg
    33841 acggttcctg caccttttcc agttacgcct gagccgtgcg catcatcgac gtaggtgatc
    33901 agtccatatt tttcaacaat cggaataatt tccggcaatc gtgcaatgtc tccatccatg
    33961 gagaaaacac catctgtgat atacatgatt tttttatatt tcttactttc tatcgcttcc
    34021 tttgcttttt tctcaaggtc tttcatatct tgatgtttaa ttcggatgat ttttgctccg
    34081 gataagcggc aaccgtcaat aatagatgca tggtttaatt catccgataa gatagcatct
    34141 tcttttgtca ttactgcaga gatagcgccc atattgcagt tgaatccaga ttgaaaggcg
    34201 attgctgctt ctgtcccttt gaattctgcg attttttctt ctaattgctg atggattttc
    34261 agcgaaccat tgatcgttcg gactgctcct gctcctacac cataagtttc ggttgcttca
    34321 atacatgctt ttttcaattc ttcgcgattg gcaaatccaa gataattgtt ggatgctaga
    34381 ttgatcatct tttttccgtc tacgatgata caagcaccat tttcactttc tagtatgtcg
    34441 atcgtattgt ataacccctt ttctttcagt tgatccaatt ctttctctaa atacattgtt
    34501 agttctttgg aagacattgt ctccacctcc atattcatct gtcttgcaga acataccaaa
    34561 catatatcgt gtcattcatc tacaaagcaa gtatgctata tttttgataa cgcttacact
    34621 gattatgaga attatttaac cacatttttt atttacagta tctttttact ttcaaatgac
    34681 ttatatagta tgaacaatta ccttttagat aatgatctat tttactctac ctgggcagat
    34741 aaatataaca caaccattga tagaagccct ttcgttaaaa tcaattgtat tctgaatatt
    34801 taatttattt attgcataga ccaataaaaa tacataaaat ttccatcttc gtttatcagc
    34861 aatttttaat tttactctct gaatatttct taaaagtttt acttgttgaa ttcgaaaaaa
    34921 ggactgtgac aaaagtcttg tcacagtcct attcttccga a
//
LOCUS       DACCXM010000014        34423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCXM010000014 DACCXM010000000
VERSION     DACCXM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.015"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            352..1041
                     /locus_tag="IVY61_000597"
     CDS             352..1041
                     /locus_tag="IVY61_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7969736.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IVY61_000598"
     CDS             1028..2236
                     /locus_tag="IVY61_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7969737.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IVY61_000599"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IVY61_000599"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP7969738.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVY61_000600"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVY61_000600"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP7969739.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="IVY61_000601"
     CDS             complement(6339..7034)
                     /locus_tag="IVY61_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP7969740.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVY61_000602"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVY61_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP7969741.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="IVY61_000603"
     CDS             complement(8233..8424)
                     /locus_tag="IVY61_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP7969742.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="IVY61_000604"
     CDS             complement(8445..9335)
                     /locus_tag="IVY61_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP7969743.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="IVY61_000605"
     CDS             complement(9322..10089)
                     /locus_tag="IVY61_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7969744.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVY61_000606"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVY61_000606"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP7969745.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVY61_000607"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVY61_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP7969746.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVY61_000608"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVY61_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP7969747.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14705..15043
                     /locus_tag="IVY61_000609"
     CDS             14705..15043
                     /locus_tag="IVY61_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969748.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="IVY61_000610"
     CDS             complement(15211..15969)
                     /locus_tag="IVY61_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7969749.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="IVY61_000611"
     CDS             complement(15987..16805)
                     /locus_tag="IVY61_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7969750.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVY61_000612"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVY61_000612"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP7969751.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVY61_000613"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVY61_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP7969752.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="IVY61_000614"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="IVY61_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP7969753.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="IVY61_000615"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="IVY61_000615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP7969754.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="IVY61_000616"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="IVY61_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP7969755.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="IVY61_000617"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="IVY61_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP7969756.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="IVY61_000618"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="IVY61_000618"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP7969757.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="IVY61_000619"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="IVY61_000619"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP7969758.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /locus_tag="IVY61_000620"
     CDS             26847..27146
                     /locus_tag="IVY61_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP7969759.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="IVY61_000621"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="IVY61_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7969760.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="IVY61_000622"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="IVY61_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP7969761.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="IVY61_000623"
     CDS             28113..30086
                     /locus_tag="IVY61_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP7969762.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="IVY61_000624"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="IVY61_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP7969763.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="IVY61_000625"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="IVY61_000625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP7969764.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="IVY61_000626"
     CDS             32965..34257
                     /locus_tag="IVY61_000626"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP7969765.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10506 a         6693 c         6817 g        10407 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25201 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25261 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26641 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34381 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaa
//
LOCUS       DACCXM010000015        33175 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCXM010000015 DACCXM010000000
VERSION     DACCXM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.016"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(44..622)
                     /locus_tag="IVY61_000627"
     CDS             complement(44..622)
                     /locus_tag="IVY61_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7969766.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(699..1229)
                     /locus_tag="IVY61_000628"
     CDS             complement(699..1229)
                     /locus_tag="IVY61_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP7969767.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1233..2093)
                     /gene="rlmB"
                     /locus_tag="IVY61_000629"
     CDS             complement(1233..2093)
                     /gene="rlmB"
                     /locus_tag="IVY61_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP7969768.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2086..2481)
                     /locus_tag="IVY61_000630"
     CDS             complement(2086..2481)
                     /locus_tag="IVY61_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7969769.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2478..3890)
                     /locus_tag="IVY61_000631"
     CDS             complement(2478..3890)
                     /locus_tag="IVY61_000631"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP7969770.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3887..4423)
                     /gene="cysE"
                     /locus_tag="IVY61_000632"
     CDS             complement(3887..4423)
                     /gene="cysE"
                     /locus_tag="IVY61_000632"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP7969771.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(4745..6205)
                     /locus_tag="IVY61_000633"
     CDS             complement(4745..6205)
                     /locus_tag="IVY61_000633"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP7969772.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6375..7532)
                     /locus_tag="IVY61_000634"
     CDS             complement(6375..7532)
                     /locus_tag="IVY61_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP7969773.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7566..8936)
                     /gene="radA"
                     /locus_tag="IVY61_000635"
     CDS             complement(7566..8936)
                     /gene="radA"
                     /locus_tag="IVY61_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP7969774.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8999..9526)
                     /locus_tag="IVY61_000636"
     CDS             complement(8999..9526)
                     /locus_tag="IVY61_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP7969775.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9756..10562
                     /gene="proB"
                     /locus_tag="IVY61_000637"
     CDS             9756..10562
                     /gene="proB"
                     /locus_tag="IVY61_000637"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP7969776.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10559..11806
                     /locus_tag="IVY61_000638"
     CDS             10559..11806
                     /locus_tag="IVY61_000638"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP7969777.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11882..12226
                     /locus_tag="IVY61_000639"
     CDS             11882..12226
                     /locus_tag="IVY61_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969778.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12343..12669)
                     /locus_tag="IVY61_000640"
     CDS             complement(12343..12669)
                     /locus_tag="IVY61_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969779.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13284..14486)
                     /locus_tag="IVY61_000641"
     CDS             complement(13284..14486)
                     /locus_tag="IVY61_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7969780.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14603..15118
                     /locus_tag="IVY61_000642"
     CDS             14603..15118
                     /locus_tag="IVY61_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7969781.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15317..15763)
                     /locus_tag="IVY61_000643"
     CDS             complement(15317..15763)
                     /locus_tag="IVY61_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7969782.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15810..17288)
                     /gene="msr"
                     /locus_tag="IVY61_000644"
     CDS             complement(15810..17288)
                     /gene="msr"
                     /locus_tag="IVY61_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7969783.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17642..18271)
                     /locus_tag="IVY61_000645"
     CDS             complement(17642..18271)
                     /locus_tag="IVY61_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7969784.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18255..18719)
                     /locus_tag="IVY61_000646"
     CDS             complement(18255..18719)
                     /locus_tag="IVY61_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7969785.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19206..20474)
                     /locus_tag="IVY61_000647"
     CDS             complement(19206..20474)
                     /locus_tag="IVY61_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7969786.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20705..21442)
                     /locus_tag="IVY61_000648"
     CDS             complement(20705..21442)
                     /locus_tag="IVY61_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7969787.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21509..22078)
                     /locus_tag="IVY61_000649"
     CDS             complement(21509..22078)
                     /locus_tag="IVY61_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7969788.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22206..23078
                     /locus_tag="IVY61_000650"
     CDS             22206..23078
                     /locus_tag="IVY61_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7969789.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23420..24292
                     /locus_tag="IVY61_000651"
     CDS             23420..24292
                     /locus_tag="IVY61_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7969790.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24289..26247
                     /locus_tag="IVY61_000652"
     CDS             24289..26247
                     /locus_tag="IVY61_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7969791.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26579..27430)
                     /locus_tag="IVY61_000653"
     CDS             complement(26579..27430)
                     /locus_tag="IVY61_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7969792.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27553..28020
                     /locus_tag="IVY61_000654"
     CDS             27553..28020
                     /locus_tag="IVY61_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP7969793.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28283..28765)
                     /locus_tag="IVY61_000655"
     CDS             complement(28283..28765)
                     /locus_tag="IVY61_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7969794.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28865..29728)
                     /locus_tag="IVY61_000656"
     CDS             complement(28865..29728)
                     /locus_tag="IVY61_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7969795.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30326..31201
                     /locus_tag="IVY61_000657"
     CDS             30326..31201
                     /locus_tag="IVY61_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969796.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(31346..31498)
                     /locus_tag="IVY61_000658"
                     /pseudo
     CDS             complement(31346..31498)
                     /locus_tag="IVY61_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(31766..33097)
                     /locus_tag="IVY61_000659"
     CDS             complement(31766..33097)
                     /locus_tag="IVY61_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7969797.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         9677 a         6927 c         5923 g        10648 t
ORIGIN      
        1 atgcaataaa aataaataag aagctcatta agatttttct aaattattcc tcattataaa
       61 atttcaagtg tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt
      121 gatccattgc taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa
      181 aaattttagc ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata
      241 aaagatgctg aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt
      301 ctgtctctat tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt
      361 tctgcttcct caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa
      421 agctaacacc tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt
      481 cttgttccca atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta
      541 aaagtggaaa atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag
      601 cgcgcaataa ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct
      661 tatcatcaat gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa
      721 tcgtccaatt tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg
      781 aaatcaataa aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc
      841 gtcttatata attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt
      901 tctgctaaat cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg
      961 attctctcga tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg
     1021 tatttcttat aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact
     1081 tccattcctt catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc
     1141 gccattttgt cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag
     1201 ccgtctacaa tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc
     1261 tgatacataa gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc
     1321 gggatcgtca gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca
     1381 ttaccaatga ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc
     1441 atatctgtgc caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa
     1501 ttcgtcacgc gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata
     1561 ggtgtgattc caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct
     1621 gctgtccgta aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag
     1681 aaggggtctt cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca
     1741 taaggtgtaa tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat
     1801 ttttgtttag gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct
     1861 tctacttttt ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt
     1921 agcgcttcta ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt
     1981 tgatcagaaa ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt
     2041 ttgtcttcaa atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc
     2101 accaactttc tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa
     2161 atgcagatag cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc
     2221 attttttgcg ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc
     2281 ttctgtcaaa agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga
     2341 aacataattc gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag
     2401 gtggtcacga acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt
     2461 caattgtgtg taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct
     2521 aataagatcc ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga
     2581 ttgcttcttg cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct
     2641 tctgctgtaa ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt
     2701 gccaagacag cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc
     2761 tcatagacca ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat
     2821 cgctgttcta aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag
     2881 gcagtttctt ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg
     2941 gctgcttctt tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc
     3001 atgaagaaac gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa
     3061 ttccctagag atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc
     3121 atccagtaat tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca
     3181 tgatgaggaa attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt
     3241 tttgttgcca taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa
     3301 tcccaagaaa tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct
     3361 ttttgctgct gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat
     3421 tggtcactta atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa
     3481 gattcataag catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata
     3541 tgatccatga ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct
     3601 tcaaatgctc ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct
     3661 gtacggatga ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct
     3721 cgatattcaa aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata
     3781 tggatataat tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata
     3841 gggatgaatt cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc
     3901 tcactttttc tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt
     3961 ctggaacatc tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta
     4021 ccggtcctaa tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc
     4081 ttttacccgt ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt
     4141 cctcgatttc tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag
     4201 ttccaatcgt tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat
     4261 tgatcttcgc caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa
     4321 acaaggcatg aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat
     4381 cgtttctctt tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc
     4441 gtaagaattt tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag
     4501 tttccttatc aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc
     4561 tctgacagct aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt
     4621 cctgttttct gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat
     4681 accttgcacc acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc
     4741 gtgtttagtt cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga
     4801 tcgtttcagg caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga
     4861 aaagattttt tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag
     4921 ttggagcatc tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag
     4981 gaactgtttc accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga
     5041 aaaatagcgt agacagatca acgatttctg cagcatagct catttgaggt tgatacaggc
     5101 taacgatctt cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat
     5161 cagcgattag ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt
     5221 aatggttgtt tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc
     5281 gttttggatc gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag
     5341 gtgaccagcc caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc
     5401 gatattgttc gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt
     5461 cactgttgat gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat
     5521 agatcatcag ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga
     5581 ttttcatcaa atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt
     5641 tttggatgac aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca
     5701 tatctgtaaa tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac
     5761 cttgtgcttc tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat
     5821 gaggcatttc tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc
     5881 aatagcattt gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt
     5941 ctttacgttc agattgacgg taagggccgt attctccagg attttcagga gattcatccc
     6001 aattcattcc caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac
     6061 gtttttggtc agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca
     6121 aataattgaa taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg
     6181 catagcgtac gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa
     6241 cagcaaaaat tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac
     6301 aaattttacc ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg
     6361 gctgaatttc ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg
     6421 attcctcgtc ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg
     6481 gcacttgtta caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca
     6541 accatcgtgc cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact
     6601 actagaacat tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca
     6661 ttgatattaa gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg
     6721 gttacaatga caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg
     6781 atatcttcaa agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca
     6841 tttaggatat ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg
     6901 atgtattgta gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca
     6961 gtctttgtaa tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat
     7021 ttatggaaat tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt
     7081 tcttgctcga tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc
     7141 gttcccattc tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta
     7201 ttgacgaatg gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat
     7261 ccgatgatcg caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt
     7321 tgttctactt gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag
     7381 aaaataagtg cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt
     7441 tgaccgattg cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg
     7501 atcatcagca aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt
     7561 attagttagc ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg
     7621 attccttttg gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag
     7681 ccgagttttt ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca
     7741 gtcagtccaa tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt
     7801 gaagcaatac tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct
     7861 gcagctttta agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact
     7921 gccatgatca gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg
     7981 aacatagtag gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt
     8041 gccacaacga tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa
     8101 ggattagcga cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt
     8161 gagccaaatc ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca
     8221 aaatataaca ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct
     8281 ttggtaacat gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt
     8341 tctgcagtgg tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc
     8401 gtcatcgttt ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga
     8461 ctgatttcgt tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg
     8521 cgttccgcgc gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt
     8581 ccgcctatgg ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct
     8641 ggatcgccac caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc
     8701 agttcttcta attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt
     8761 gttggttttg ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt
     8821 tcgatttctt cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta
     8881 ggcgacacat acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta
     8941 ccgcctcctt ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt
     9001 atttactgga agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt
     9061 ttaaaaacgg caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct
     9121 tgaaaccaaa gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat
     9181 aatcactgtc aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc
     9241 ttgaacggtt agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag
     9301 gcacgagggt tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg
     9361 cgactgtttc caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt
     9421 ctgctgcttc aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat
     9481 tttgatcttt atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct
     9541 aaataaacta ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct
     9601 tcaagaaatt ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca
     9661 agttgtacaa aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac
     9721 gaaattaatg agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa
     9781 aacgaattat tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc
     9841 ttgcggcaat cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag
     9901 tgatcttagt ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac
     9961 gtccgacttt gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga
    10021 atatctataa tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa
    10081 gagacgtggt agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt
    10141 tgattatgga cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag
    10201 atcatctgac aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg
    10261 cggcagacct tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta
    10321 caaatcccga ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac
    10381 aagcaggtgg tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg
    10441 cttctaggat atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa
    10501 ttatttttga tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat
    10561 gactgacttg atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat
    10621 gcctacaaac cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca
    10681 acaagcgatc attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag
    10741 tgaaacgatg caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga
    10801 gatcgaaaaa attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa
    10861 caccgacgga ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta
    10921 cgaatctcgt ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc
    10981 cgttatcctt cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt
    11041 gcagcaagta ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac
    11101 ttctcatgaa actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc
    11161 tcgaggcgga gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga
    11221 aacaggtaca ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt
    11281 gaacatcatc gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt
    11341 gctgatccat tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga
    11401 acacggggtc tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt
    11461 agcagaagac acagattggg atactgaata tctagattat atccttgctg tcaaagtggt
    11521 cgattctttg gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac
    11581 gatcgtgaca gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc
    11641 tgtctatgtc aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga
    11701 gattggtatc tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac
    11761 ttccacaaaa tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa
    11821 cattattccc gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa
    11881 tatgtttgaa attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt
    11941 tctgggagtc ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca
    12001 acgggaaaaa tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt
    12061 gcacattagt tacatggttg cattatgctt attagcgaca atcaattact attctttttt
    12121 cgatcattca atcgcttata cgattacgct actgattgct gggatttttc acttatctta
    12181 taaatatcag ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg
    12241 ataagtagag tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa
    12301 agattttgtc acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa
    12361 atctgtttcc tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg
    12421 aagcagctca tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc
    12481 cagtagctgt tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct
    12541 agtttcacct tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg
    12601 atcatctgaa aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc
    12661 ttccagcatc tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa
    12721 aattttccag tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc
    12781 ccctttttcc gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc
    12841 aacaaacatg agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt
    12901 ctcacaacct cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac
    12961 ggtaaaatat gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg
    13021 attttacaac ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa
    13081 cggcaaaata tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg
    13141 attttacaac ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac
    13201 caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca
    13261 gctttttcac aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga
    13321 taaatgatac aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt
    13381 tcaccaatat gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag
    13441 cggacaaaac tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca
    13501 atcgtcgtca acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc
    13561 ggtacaagga ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag
    13621 acaaatgctg ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg
    13681 aaaatcgaag caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat
    13741 tcagaatagc caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta
    13801 tataacaatg cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct
    13861 tcccaaaggc ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct
    13921 ataaaaccgc aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg
    13981 cccaagaccc caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac
    14041 atcatgattg ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta
    14101 gagacaaata aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata
    14161 ttcccagata atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat
    14221 gtttttttca aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg
    14281 actaacatgt aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca
    14341 gcaatcgttt ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta
    14401 gctgctacaa ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata
    14461 tctatttgat tctccaatat ttccatccct ttctctctat tagcatcata catgatttat
    14521 tttacatgtc aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa
    14581 taagacgaga ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt
    14641 atccaaacca cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca
    14701 tcacagtcag atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt
    14761 ggacaaagac gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct
    14821 tgtagaggaa tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc
    14881 aaaaatctat gctttttcga cgctcgataa aacaacagct actggactgc tattcactag
    14941 agaacaacag ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct
    15001 agacaagaaa caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca
    15061 gcaggaactc caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg
    15121 gataaacaga acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc
    15181 aatacagttg gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca
    15241 ctatttttct agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc
    15301 aaataacagt gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg
    15361 ggccaacgat gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca
    15421 tataagcaga gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca
    15481 tacatgtcac cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc
    15541 tttctggatt tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga
    15601 catccaaaat gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat
    15661 gaagatcttg atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca
    15721 tcattgttgt aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta
    15781 aatttgtcac gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt
    15841 ttctgccctt ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga
    15901 gaagtaaaca aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt
    15961 gccatatcaa taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa
    16021 agcaaagcaa tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc
    16081 aaagtttcct gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac
    16141 tctgtttgtc tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt
    16201 tgctgatata cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg
    16261 atatgttcta ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta
    16321 cctaaagaaa actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg
    16381 acattttgtc ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa
    16441 cggatttgtt tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct
    16501 aatgttttcg cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac
    16561 aaccgatcca gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc
    16621 gcttgttttt tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac
    16681 tctgcttctc gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa
    16741 ttccccgaaa actctcggat ttctccgtct tttacttccc agattttcga tgctagctca
    16801 tctagaaaat agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct
    16861 tcaatcagtc gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc
    16921 ccttgttcat aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc
    16981 catttccgct ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt
    17041 aggtcctttg cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt
    17101 tggattttcc catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta
    17161 ccgacaccat tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca
    17221 atagatagtt ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa
    17281 ttttccatat gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag
    17341 agcagaaaaa tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata
    17401 actatgacta aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa
    17461 atggacatac tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg
    17521 ttattcaaaa gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt
    17581 attaggttac cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct
    17641 tttattcgaa catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta
    17701 ccgcttttcc tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg
    17761 cctctgccaa ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac
    17821 gcgcaactaa catgaattgc atagccgcta agctagaatc gactttgact acatcgttca
    17881 tttcttctct tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga
    17941 cttcttttgg catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt
    18001 aacattgcat atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat
    18061 tgaaacggat caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc
    18121 gccatggttt catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca
    18181 tttcttcgtg tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca
    18241 taattgttga aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt
    18301 atattgcaaa gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca
    18361 tcgctgaaag ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg
    18421 attttcttct gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt
    18481 ctctgacgcg aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct
    18541 tgatcaatct gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt
    18601 ctcatcatga tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg
    18661 attttttgat cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata
    18721 atttcctcct ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa
    18781 aagaaaaaag tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata
    18841 gagaaaagaa attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa
    18901 accaagatta ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt
    18961 acaccgccaa aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg
    19021 gatcagtatg cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt
    19081 gagtaaacgg tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat
    19141 gaaaatctgt ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc
    19201 tctttttaat aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa
    19261 tctctttcct tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat
    19321 agtcgataaa ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg
    19381 atttttgtta aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa
    19441 ggattgtata aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca
    19501 tattcccagc ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt
    19561 tctcctgtat gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca
    19621 tactgtgtca gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca
    19681 gtggcataac gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc
    19741 catgcaccgg aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg
    19801 ttatcttgca atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc
    19861 cattgcccat tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct
    19921 ttaatcccaa atagattgta attaggggca gaagccaaag tactgttccc ccagccactt
    19981 tcaacgatgg cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt
    20041 tgtgcagttg aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca
    20101 taaaaagcat agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc
    20161 aatgtttgcc catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga
    20221 gcttctgctg atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct
    20281 tcggttgaaa ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt
    20341 tcaattcctg tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca
    20401 gctagcactg ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa
    20461 ttctttgctt tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta
    20521 actaaaaaag taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata
    20581 aatttgcttt ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt
    20641 aaattgatgg tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta
    20701 tggattatac ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg
    20761 gcgtttctat cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt
    20821 tttgcgcttc atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag
    20881 tttgaacagc tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac
    20941 gtaatccttc catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag
    21001 gataaacaac atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt
    21061 ccatgattgg aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca
    21121 tattttgcca ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat
    21181 tattgtacaa tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga
    21241 cttgtgcaaa atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt
    21301 cttttattgc ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt
    21361 ctgctaatag cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca
    21421 ctttttctga taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata
    21481 ctcactatct cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt
    21541 tggcagggat tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg
    21601 ctgcaacaat ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga
    21661 tccaagcatt tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat
    21721 cggttagatg gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta
    21781 aacagatgcc acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta
    21841 tactaatatg tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta
    21901 ctttgtcact aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag
    21961 tattcagcgc tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga
    22021 gcggatccgt tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt
    22081 tgctccctcc ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa
    22141 acatacttaa ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg
    22201 aaaaaatgga tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca
    22261 gccgagcagc aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt
    22321 tagaagaaga acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg
    22381 attctggtcg atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg
    22441 cgaacaattt aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa
    22501 ctaaagcaat ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata
    22561 tccagtttca tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat
    22621 tacttgattt tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac
    22681 tgccagccca agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa
    22741 cagtcatcca tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag
    22801 tggacagtca actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa
    22861 catataactt attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag
    22921 ctctagatgg gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc
    22981 cattacaagc ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc
    23041 gtaaattttt agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt
    23101 tcagcttttt aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt
    23161 cttaatttgt tccttccttt tatttctgat aattccagta aattactata attataaaaa
    23221 aagaattgac aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc
    23281 atgtgactag atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt
    23341 ttttcgtgtc tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat
    23401 cttcaaaaag ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt
    23461 agatgacggt ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag
    23521 aaatgatttg atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca
    23581 aatcaagttg caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac
    23641 gatcattgat catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc
    23701 tttgaccgac cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa
    23761 caaactttta agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc
    23821 agtagagtat ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat
    23881 cgcaatccat attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga
    23941 agtgacgatt gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca
    24001 ttcagaacca ttttcactga attattcaag actggtgaat catttacgac tgcttctaca
    24061 acgaacccat gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg
    24121 aaaataccca gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata
    24181 ccagctcgcg atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag
    24241 aagcgcaatc gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat
    24301 ctacaacgaa tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta
    24361 ctagtaggga tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta
    24421 caagctggcg gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca
    24481 ctagggatgt cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa
    24541 gttccagtaa atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa
    24601 tcagttgatc catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg
    24661 attgcggcag ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc
    24721 tttagcggca agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca
    24781 gtgctactgc tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt
    24841 ttgagtttag gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct
    24901 acaggattgc atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat
    24961 attggtaaat ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga
    25021 tttttcccaa tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca
    25081 cgtccagaaa acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc
    25141 tttactggtg taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat
    25201 gtcttgcatg caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca
    25261 gcaggatttg cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt
    25321 gcgaatcagc cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt
    25381 ggctttaact ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat
    25441 ctttcagatg acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc
    25501 aaaatctacg ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca
    25561 cgccttcgtt tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact
    25621 ggcgtacctg gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa
    25681 gtacaattcg ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca
    25741 caaacaaaca ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt
    25801 tacgctatcg caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg
    25861 gaaaaaatga tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca
    25921 atcgctggta cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat
    25981 gctggtatcg aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca
    26041 ttcactttat atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt
    26101 gatcttgccg ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat
    26161 ggcgacaaag tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa
    26221 atcggctctg tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa
    26281 acaaacagga gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt
    26341 tctcctgcca actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt
    26401 tatttgactc ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt
    26461 gttcaaaaac cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt
    26521 tcacaaaatt tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt
    26581 attcctgatc caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata
    26641 ctttttctaa atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg
    26701 ctaaaaacat gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg
    26761 ctgcatcact tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg
    26821 ctaattcttc atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt
    26881 ctactagtac ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc
    26941 catttcctgc agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt
    27001 cattctctac ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca
    27061 ttttttcagt gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa
    27121 aacttgtata agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat
    27181 gttcggcttg acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca
    27241 acgcttcttt cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt
    27301 ctttcagtgc ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa
    27361 ccatcttttt taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta
    27421 aataatgcat atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt
    27481 tataaattac acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa
    27541 ggatgacaac tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat
    27601 attgaaatgt ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa
    27661 acaaatgcag caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt
    27721 acaaaaaccg gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta
    27781 gatgtctatc gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat
    27841 ccaaattgcg tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg
    27901 cagaaaaagg cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata
    27961 gccgttttgg aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa
    28021 ttctctctac tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct
    28081 ccgggggaca aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct
    28141 gaagtcatga cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa
    28201 aaatgcaaaa tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc
    28261 tactttcacg acctctatat tatcatggtt tattgactac cggtatccag atttctccca
    28321 tatatttccc gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt
    28381 aatcttctac ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt
    28441 cttcaggcga ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga
    28501 ttcgggtagt gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc
    28561 cgttataggg ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat
    28621 ctattgttcc attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga
    28681 caaaatccgt attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt
    28741 tttcttcgat cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt
    28801 acataaaatt aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt
    28861 ttaactattt ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct
    28921 ctccccattt tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa
    28981 gccattctgc atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat
    29041 taggaaaaat ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat
    29101 tttcttttgt cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg
    29161 ctccatcaaa tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat
    29221 gcatctcttc cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga
    29281 ctccttcaaa tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct
    29341 tttcgatacg aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg
    29401 gaaaagattt ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac
    29461 gaaaagctct tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca
    29521 ctcttgatcc atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt
    29581 ccattagcga catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc
    29641 ctgtattttc ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat
    29701 cgatcaattg gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca
    29761 tattatcaaa gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa
    29821 atgttgtatg gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta
    29881 tctgcctatc tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta
    29941 aacaaagcgg taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca
    30001 aagcaaattg ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca
    30061 tcatacctct tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg
    30121 tcagataaat aaaaaacaaa aaaagttcca tctttaaaac acgctattct tttacttttt
    30181 ctcacattct ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat
    30241 tatcaaatat gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat
    30301 aagtgtttca tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt
    30361 tttggaacgc ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag
    30421 caagtacttg tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga
    30481 tgaaaagacc aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca
    30541 catggctgct gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact
    30601 ttccgaactc tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac
    30661 agagattctt atagaacatc cgcaatttat cgaacgcttc aaagttaaga tgccatttca
    30721 tgcaataaat tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa
    30781 gaaaaacaaa aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga
    30841 agcagttgaa tcgctcattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga
    30901 agtgattgct actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct
    30961 gatagaggac tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa
    31021 aaggcaaatg ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa
    31081 acgctactcc ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa
    31141 gaaaaaaata gataaaaaaa ctaaagaaac ggtaaagaaa gacgatttca atgaaaaata
    31201 gcaagtaaac gataacaacc atcctaaatc cacaagaaat acagcagacc ttgctttaaa
    31261 aacacgaatc atgcaaagga tgaagtaaag aagacttaac gcgtacccaa tattagtcat
    31321 ttgactatta ggtacgcgtt ttgttttata agtcatgaca aatttccttc gttgctgggt
    31381 agattttccg gcattgttga tagattttat gatacttttc tacattttgt aagagaggtt
    31441 ccactgcatc tgtatattgg ataaaattct gacaacaact ttcaggtgtg tcgaaccact
    31501 ctaatcttta aagataaata ttaaaataac gattaataat attaacagtc tttatttttc
    31561 accaccaaaa acctatcttt gcatatacta aatagacgta tatacacaga taggtttgtc
    31621 ttgtgaaaaa taatgattat ccttaccaaa aaatgtttgt ttttctacat gttcgtactt
    31681 atcaaaaaat atgatcctta ttgaaattac tggctattta tattccaacg acttcttgtg
    31741 ttaatttttg ttatctcata gtaaattatg tttcattttc acttaattta gttgtcacgt
    31801 gttcgtcaat tcgtaaaaac ggtaaatata taaacacact tgcggctata attaatattt
    31861 gtaaaacagc tgctcgccag tctccggctg ttgctaaaaa tcccgatata actggtggcg
    31921 tagtccaagg aatttgcact actacatgat taattaatcc cacagcagtt gcaaaatatg
    31981 ctactgtcaa agaaaaaatc ggtgcaagca caaagggaat aatcagactg atattaaata
    32041 caataggcaa tccaaaaata ataggttcgc ttatattgaa tatagcggga gttaccgata
    32101 atttagcaaa gtccttataa tcttttctac gactgaagat aaatattgca atcaataaag
    32161 caatcgtatt tcccgatcca cccattaccg caaaggtatc tttaaaggcc atattgataa
    32221 tatgtggaat ttgctctttg tttgcataag ctgccatatt ttcttgcata ttctgaatca
    32281 aaaacggatc taacaatgaa cctgatatta ctgcttgatg gataccaaat ccaaaaaata
    32341 gattagcaat agatgtaatt aacaaaaatc ctggtaaacc agttgtaatt ttacttaatg
    32401 gctctgtaac tatcgtggta attagcttat ttatatccat attaaacaat tgaatgatta
    32461 taaatgacaa taaagcaaac gaggtaatcg taataatgat tgggattaat acattaaaag
    32521 actttaaaac agcgggagga atatttccgg atagctgaat ctgtagtttc ttattattag
    32581 aaagtttaat aaataattct gtagccgcta atccaacaaa tatacctaca aacattcctg
    32641 aagctcctgt ataggatata ggtatcacac ccgtcacttc aacagcttta tccgcatttt
    32701 caggagtaaa catcatctgc ataggtgtta atacaacaaa tgtcgaaata gcaactaata
    32761 ccgctgcaat atgatttgta taatttctgc ttgcacacat atgataagat actgctgcta
    32821 caactaataa tgtagttatg cttaatgtac cattggcaat tgaagtacca atttgttgta
    32881 acgtggtcaa tgtactagta tcgacaattt cccccatgaa tcctgttggt tctaagatca
    32941 ctgaattaat aagtgtcaca aatcctgcta acatcataaa tggcataaat gtagcaaacg
    33001 catctcgcaa agttcttaaa tggatctgat tccccaactt agtcgcaact ataccaaatt
    33061 tttcaaatac aatgtccttc ctgctacttt tgcccatatt ttttctcctt tatgcttatt
    33121 gtaatcgatt actttttcca cccaaataat acatcactta tcaaaaaaag taaat
//
LOCUS       DACCXM010000016        32171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCXM010000016 DACCXM010000000
VERSION     DACCXM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.017"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(109..489)
                     /gene="yajC"
                     /locus_tag="IVY61_000660"
     CDS             complement(109..489)
                     /gene="yajC"
                     /locus_tag="IVY61_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP7969798.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(586..1731)
                     /gene="tgt"
                     /locus_tag="IVY61_000661"
     CDS             complement(586..1731)
                     /gene="tgt"
                     /locus_tag="IVY61_000661"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP7969799.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1949..2647
                     /locus_tag="IVY61_000662"
     CDS             1949..2647
                     /locus_tag="IVY61_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7969800.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2815..5454)
                     /locus_tag="IVY61_000663"
     CDS             complement(2815..5454)
                     /locus_tag="IVY61_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7969801.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6073..7104)
                     /gene="queA"
                     /locus_tag="IVY61_000664"
     CDS             complement(6073..7104)
                     /gene="queA"
                     /locus_tag="IVY61_000664"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP7969802.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7297..8472
                     /locus_tag="IVY61_000665"
     CDS             7297..8472
                     /locus_tag="IVY61_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7969803.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8476..9117
                     /locus_tag="IVY61_000666"
     CDS             8476..9117
                     /locus_tag="IVY61_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7969804.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9114..10031
                     /locus_tag="IVY61_000667"
     CDS             9114..10031
                     /locus_tag="IVY61_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7969805.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10028..10699
                     /locus_tag="IVY61_000668"
     CDS             10028..10699
                     /locus_tag="IVY61_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7969806.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11240..11728)
                     /locus_tag="IVY61_000669"
     CDS             complement(11240..11728)
                     /locus_tag="IVY61_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP7969807.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11929..12195)
                     /locus_tag="IVY61_000670"
     CDS             complement(11929..12195)
                     /locus_tag="IVY61_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7969808.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12195..12458)
                     /locus_tag="IVY61_000671"
     CDS             complement(12195..12458)
                     /locus_tag="IVY61_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7969809.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12451..12723)
                     /locus_tag="IVY61_000672"
     CDS             complement(12451..12723)
                     /locus_tag="IVY61_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969810.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12934..13119
                     /locus_tag="IVY61_000673"
     CDS             12934..13119
                     /locus_tag="IVY61_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7969811.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13261..15084)
                     /locus_tag="IVY61_000674"
     CDS             complement(13261..15084)
                     /locus_tag="IVY61_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP7969812.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15682..18387)
                     /locus_tag="IVY61_000675"
     CDS             complement(15682..18387)
                     /locus_tag="IVY61_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP7969813.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18693..19640)
                     /locus_tag="IVY61_000676"
     CDS             complement(18693..19640)
                     /locus_tag="IVY61_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP7969814.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19830..20099
                     /gene="rpsN"
                     /locus_tag="IVY61_000677"
     CDS             19830..20099
                     /gene="rpsN"
                     /locus_tag="IVY61_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7969815.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20275..20637)
                     /locus_tag="IVY61_000678"
     CDS             complement(20275..20637)
                     /locus_tag="IVY61_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7969816.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20648..21769)
                     /locus_tag="IVY61_000679"
     CDS             complement(20648..21769)
                     /locus_tag="IVY61_000679"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP7969817.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21785..22135)
                     /locus_tag="IVY61_000680"
     CDS             complement(21785..22135)
                     /locus_tag="IVY61_000680"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP7969818.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22265..23776)
                     /locus_tag="IVY61_000681"
     CDS             complement(22265..23776)
                     /locus_tag="IVY61_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7969819.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24098..25450)
                     /locus_tag="IVY61_000682"
     CDS             complement(24098..25450)
                     /locus_tag="IVY61_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP7969820.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25603..26679)
                     /locus_tag="IVY61_000683"
     CDS             complement(25603..26679)
                     /locus_tag="IVY61_000683"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP7969821.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26875..28620
                     /locus_tag="IVY61_000684"
     CDS             26875..28620
                     /locus_tag="IVY61_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP7969822.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28900..29337)
                     /locus_tag="IVY61_000685"
     CDS             complement(28900..29337)
                     /locus_tag="IVY61_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP7969823.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29321..30694)
                     /locus_tag="IVY61_000686"
     CDS             complement(29321..30694)
                     /locus_tag="IVY61_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969824.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31016..31504)
                     /locus_tag="IVY61_000687"
     CDS             complement(31016..31504)
                     /locus_tag="IVY61_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7969825.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31526..32128)
                     /locus_tag="IVY61_000688"
     CDS             complement(31526..32128)
                     /locus_tag="IVY61_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7969826.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9196 a         6879 c         5940 g        10156 t
ORIGIN      
        1 attcaatatt gagcaatata tcccatgctc ttatctcttt cttttttgaa taaaaaatga
       61 ggtaggacat ctttctgccc tacctcgtct ttaaataagc tttgatactt atttttcttc
      121 cttctcttct gtctcttcca gcgtttctgt tgtttcagtt tttacttcaa ctgtctcagc
      181 tggttcttct gttgtttcaa cagtagtcgt tgcatcattt gttacgacag tccctggttt
      241 gaccgttttg attgctgcac gatcgtactc aaggaagatc ccttcgcagt ctaaaacaac
      301 tgtccgtttg tcattgtcga tttctgaaac aactccatgt aatccgccga tcgttacgac
      361 tgaatcacct ggtttcatgc tatctaacaa gttttgacgt tcttgctgtt gtttcttttg
      421 cgaacgtgac ataaaaaacc acattgctaa tagcgcgata aacatcacaa gcattggtaa
      481 tgttcccatt tttttcacct cactaattct gtatctacaa aaaacacttt agcagaaaaa
      541 tgctgataac actagttttt tacttaattt ttaaaagatt ttcctttaga agctttttgc
      601 gttttcttta ttgaacccgt attcttcaaa aaaggcttct ctaaattcaa gcaaattatc
      661 atccatgatt gcttgacgta cttgtttcat tagattcaac aagaagtata aattgtggta
      721 agaagtcaaa cgaatcccaa atgtttcatc acatttgatc aaatggcgga tataggcacg
      781 tgtatagttt ttacatgtat aacaatcaca tttctcatca attggacgga aatcatgggc
      841 atatttcgca tttttcacta ctagacggcc ttgagaagtc atacatgtgc catttcgagc
      901 gattcgagta ggcaagacac agtcaaacat gtctactcca cggataacac catcgatcaa
      961 tgaatcagca gcaccgacac ccatcaagta acgtggtttg ttttcgggga tcaacggtgt
     1021 agtgaattct aatacacgat tcatttctga tttaggttcc ccgacagata acccgccgat
     1081 tgagtaacca gggaaatcca tcccgactag atctttcgcg ctttgacgac gaagatcttc
     1141 aaaccctgct ccttggatga ttccaaataa tccttgacgg tctggattag catgagcttt
     1201 caatcctcgt tctgcccatc gagacgtacg ttctactgat tttttcacgt agtcgtaact
     1261 ttcatcgaat ggtggacatt cgtcaaaact catcatgata tcagagccca atttattttg
     1321 gatgttgatt gctttttctg gtgataaaaa catacgcgaa ccgttcaagt gatttttgaa
     1381 atgcacgcct tcttcttcga tattccgcat atcacttaat gaaaaaactt gaaagccgcc
     1441 tgaatctgtt aaaatcggtt gatcccagtt cataaattta tgcagtccgc cagcttcttc
     1501 tactaagtct tcaccagggc gtagccataa atgatatgta ttacttaaaa taacgccagc
     1561 acccatttct ttcaactctt ctggggacat tgtttttact gtagctaatg ttcctactgg
     1621 catgaacatt ggtgtgggga aagttccgtg tggcgtgatc aattccccta aacgcgcgcc
     1681 agtatgtttt tctttcttga ttaaacgata gcgaatggca ggttgcgtca ttcatttccc
     1741 tactttctat ataaaatatc ttgacaactc ccaccatcat aatcgtttca agtgcttttt
     1801 gcaagttccc tcggcttcct tctactttat ttaatattct tttttttatc ctttttttat
     1861 gaataacaaa aaaaagaaaa acattatgac agaaatattt tttgatacat ggtatattgg
     1921 tgttattaaa gaaaaggagg gaaaaatcat gcaatcttct gcagaattaa aacgccaagc
     1981 aaaagagagt ctaaaaggac gttggggaca agctgtttta ctaaatctaa tccctacatt
     2041 gattggtatt gctactattc ttcttattat gattcctatt atcttgtttg ccaccaccat
     2101 atatgatgga tcagcgagcg tctcagaagc gacaacttca ggaggcggta gttcaggagg
     2161 cggtggcgga attatttcca gcatcattag cgctttgttc atgagcggta tttcttggac
     2221 ttatcttgat attttacgtg gacaaaaaga tcagatcgaa ccattcaaag atgcatttcg
     2281 tggattcaaa ggtttgttta tcggcggagt gatcagctta gctgtgctga tctcgatctt
     2341 tacaacatta tggggtttgc tattagttat tcctggaatc attaaaagct attcttattc
     2401 tcaaagctat tttatttact acgatatcgt ccaagaaaca ggacaaacac ctaaagtact
     2461 ggatacgatc actgcaagtc gaaaattgat ggatggttat aaaggacaac ttttctggtt
     2521 agatctttca tttatcggct ggcatatctt agcattagca actgctggta ttggttatct
     2581 atggctcagc ccatacattt ctgcaacaaa agcagcattt tatgaacaat tgccaaaaga
     2641 tatttaaaag agagtgtgac ttgtgaccga gggggtggca ggagaacacc gtttatcgag
     2701 aggcagtaaa agaaacattc agcccattag caaaaaggag gtgtgacaac attcatgtca
     2761 cacctccttt ttgtatagtt ccagtaaaaa gaaaatcaac ttagaaacag ttaatcattc
     2821 ctcttcttct gcttccggta atcgatggaa cttgatcagt tcattcaccg cgattggtac
     2881 gatagataaa atccctacca agaaccagtg attcaaactg atgtgtacta ggccgaatag
     2941 ttcttgcgta aatggcagca aagcaacagc cgttgtaata gctaatgcca gtaacgccat
     3001 ttctaccaaa gctttattgg tcgctaaatt gactttgaag atcgattggc tgctgcgtac
     3061 attgaacaca tgcagaatcg atgaataagc taagatcaag aatgcaacag tttgaccgac
     3121 atgctgactt gcgtctaccc atgggctgat agtatcgaca taactaccta aataataacc
     3181 aaacaaggtt acgatggtga agacccctgc atttatggca attttttgca ataaacctcg
     3241 agcaaagatc ccttcatttt tcggaatggg tggctgatcc atgatgccgt gttccgctgg
     3301 ctctcttcct aaagcaaatc ctggaagtcc atcagcaact acattgacaa acagtagttg
     3361 cacagctgta aatggtgctc cccatcctaa cagcatcgca atcaaaacaa taaagatctc
     3421 cgaaatatta cagcttagca aaaagttgat agctttacga atattctgat aaaccgaacg
     3481 tccttgactg acagcgtcta cgatcgtcgc aaagttgtca tccgtcaaaa tcatatctgc
     3541 ggcactttgc gccacatctg ttcctgtgat ccccatggca cacccaacgt tacttgcttt
     3601 caacgctggt gcatcattca caccatcccc agtcatcgct acgactgcac ccgtacgttg
     3661 ccaagatttg acgatccgga tcttgtcttc tggcgttacc cgagcataga cagagagagc
     3721 tttgacacgc gcatctaatt cttcgtctga taattgatgc agttctgatc ctgaaagtgc
     3781 ttctgacgga tcattcaaga tacctaattc tttcgctatg gcactggcag tcaccacatg
     3841 atctccagtg atcatgactg ttttgatgcc agcctttttc gctcgcttga ttgctccttt
     3901 actttctgga cgtggtggat cgatcattcc gatcagacca atcaaacgta agtctttttc
     3961 cagtgcttca gaagaaatat cttgcggttc ttcgtcataa gtagcataac caacagcaat
     4021 cactctcagt gcccgtttac caaagcgatc attaacgatc gccgcttgtt ctacatcgcc
     4081 tgaactaaag cgcggagcaa gaacatcgaa tgctcctttg gtgattgaga tgtatttttc
     4141 gcccatttgg tgaactgttg tcatcatttt tcgttcagaa tcaaaaggaa gctcgtttac
     4201 tcgtggatat ttttcttcta attctgcctt tgtatgataa ttctcttcaa cagcccttac
     4261 gatggctact tctgttggat tacctttgat aacaaggtcg tcatcttctt gctcgaccgt
     4321 cacatctgta catagtgcag ccattttcaa tatttccatc gcgctgttcg tcattgaatc
     4381 ttcgatattc gttacttcat cttctttgct ccatacccgg cggacgcgca tttcattttg
     4441 tgtcagcgtt cctgttttgt ctgagcagat aacattagcg gttcctagcg tttcaactgc
     4501 tggcaactgt cggatgatcg catgtttcct cgccatcttc tgcacaccgt atgcaagtgt
     4561 caacgtaaca ataacagtca aggtttccgg tacagctgca acagccaagg aaatcgaagt
     4621 catgaacatc tcaaggagtg gttctccttg cagttcacca atcacaaata ctaaagccgc
     4681 agccgctaaa gcgatcatac tgattcgttt gcctagctga ttcaatcgga cctgtaaagg
     4741 tgttttttgc atagtatttt cgttcaaaag atccgcgatc ttacccattt ctgtagccat
     4801 gccaatggca gtgacgatcg ctttcccacg acctgcagca accgtacatc ctttaaatac
     4861 catattaagt tggtcaccta atgactcatc ttcttcagca ataaatgctg catctttttc
     4921 aactgcttca ctttctccag tcaacgccga ttcttctaca cgtaattgtg atgcttcgat
     4981 caaacgggca tcggcagaaa tagcatcacc ggactctaaa acaagaatat cacccactac
     5041 cagttgctct gactcaacag tggtcacttt gccgtctcgt atgacagttg ccgtttgttt
     5101 attcatatct tgcaaggatt ccagtgcttt ttctgcgttt ccttcttgga caatcgccaa
     5161 taccgagttg atcacaacga tagcgatgat cagcagtcct tcaaataaat ccccatgttc
     5221 tgtagcaaat gcggtataaa aggaaatcac cgctgctaca agaaggatga tcgttgtaaa
     5281 atcggacaag cttcgaaaga attttttgat catcgattct ttcggcgcct catcgaattt
     5341 attcaatccc tgcttttttc gccgttcatc cacttcttgt gaagacaacc cagtatctgg
     5401 atctgcttct gttaccttct ttacttcttc tattgtctgc ttgtattctt ccatcctatt
     5461 cctcctttaa aatcttcgta caaaaaaagg tacttatttt actataacat ccttttttta
     5521 tttacttcaa ataaagggct tatcgattat ttgttaagaa agcgtaaaat ttaaagtgct
     5581 tatatttgca aacgctacca aaggatgcta ttatatagat gtaaggaagg aggataccat
     5641 caaatagaca gaagaatttc tcgatagaac agtgtaagca aacaaagttg ttagaagtac
     5701 aggtttcacc cagacatgcg tgtagagtac aaacctataa atagtgaatg cgtatggatg
     5761 tttggatcac gaagtatccg aaacaagaag taatgcgata cgtaacaatt gaataacgta
     5821 aacgtctgaa aattgaatag gaacaagaga tgtttcaaat gttgaagatg taacgaaaac
     5881 aaaatttttg tttaacatga agaaattcaa caccgtgctt tgcatgtatg atggaaagtt
     5941 acaatcattt ctgtttattt gaatccttcc gaggtacaag aaagccgagc ttatcgagag
     6001 ctcggctttt tctttgtcat aaaatctttg taaagctttt ttgttattca aaactagcgg
     6061 cttttcataa ccttatttca cgaacatcgc atctccaaaa ctaaagaaac gatagcgctc
     6121 ttcaatcgca tgctggtaag cagataaagt caattctctt cctgcaaagg cactaactaa
     6181 catgactaaa gtcgattttg gcaaatggaa attagttgag aaagcttcta ccactttgaa
     6241 ttcatagcca ggcgtaatga aaatatcagt ccatccgctg tctgctttga tttctccatt
     6301 aaatttcgtt ccaatcgttt ctaatgttcg aattgaagtt gttccgacag ccacgatttt
     6361 cccgccattt ttacgtactt ggttcaatcg ctcagccgct tcttccgtca aacggtagaa
     6421 ttcactatgc atatggtgtt cttcaatttt atctacgctg acaggtcgga atgtacctaa
     6481 ccctacatgt agtgtcagat agactaattc aacacccttt gcttcgatct catcaagtaa
     6541 ttctttcgta aaatgcaatc cagctgttgg cgctgcagca gaaccatttt ctttcgcata
     6601 gacagtttga tagcgatctg ggtcttccag acgttctttg atatatggag gcaatggcat
     6661 ttctcccaat gattcaagaa tctctagaaa aattccttca tactcaaagt caatgatccg
     6721 tccgccgtgt tccaattctt ctttcactac tgcttgcaat ctgccatcac caaaagagat
     6781 tcttgtccca actttggcac gtttcgcagg tttgatcaat gtctcccacg tatctccttg
     6841 tgtattcgtt agaagaagga cttctaaatg cgcaccagta tctggttttt ctccatataa
     6901 acgagcaggc agaactcgtg tattattcat aactagcgca tcaccaggct gtaactcttc
     6961 aatgatttca tggaaatgtt tatcttccat ctctccagtt ttccgatcca aaacaagtaa
     7021 acgagaatga tcgcggtctt ttaatggtgt ttgtgcgatt aattcttccg gcaagtcaaa
     7081 atcaaaatct tctgttgtta acatatcgtc actcactttc tatcatgtgc ttcatcattc
     7141 tatcatgttt ttcgttctgt tggcaaaatc agcacttttt cacttctatt ttttgttttc
     7201 ctaaataaaa aaactaccct atttgtattt tttcattgtt ttaggcatac tagtaataga
     7261 tagttcttca ttgaaaatag aaaaagaggt ggcaccatga ttgaatttca aaatgtgtca
     7321 aaaatttata aaggtggaaa agtcgctgta gaggacgtca atctttcatt tgaaaagggt
     7381 gagttcattt gttttatcgg tacgagcgga agcggaaaaa cgacgacgat gcggatgatc
     7441 aaccgaatga ctgatccaac acaagggaaa attttgattg atggaaaatc catccaagaa
     7501 attgatccag tggaattgcg acgtcagatc ggctatgtta tccaaagtat tggattgatg
     7561 cctcacatga cgatccgtga aaatatcact cttgtccaaa aattgttgaa agtcagtcaa
     7621 gaagaacgga ataaaacagc tgaaaaaatg attgatcttg tggaattgcc ccgtgagatg
     7681 ttagatcgct acccacatga attatctggt ggtcagcagc agcgtattgg agtcgttcgt
     7741 gcccttgcag cgaatcaaga tatcatcttg atggacgagc cattcggtgc gcttgatcca
     7801 atcactcgtg attccttgca agaccttgtc aaagatttac aagagcgttt gggaaaaacg
     7861 atcgtatttg taacccatga catggatgaa gcgattaaac tagcaagccg tatcgccatc
     7921 atgagtgaag gccgtgtgat ccaatttgat acaccagaaa atattttacg tcatcctgca
     7981 aacgatttcg tagaagagtt gattggagaa gatcgcctac tacaagccaa gccaaatgcg
     8041 acaagagtcg gcgaagttat gctaaataca gcaatcacga ttacaccgga aaaatcatta
     8101 caagaagcaa tcaaactgat gagggaaaaa cgtgtagata cgttgttagt taccgataat
     8161 tcgaatgtat taaaaggatt tatcgatgta gaaacgttga ataaaaaacg aggaaaagta
     8221 tccagtgtcg gcgacatttt gaataaacat gttttttacg taaaagaatc cgcctatctt
     8281 cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat acgtgccggt cgttgatgaa
     8341 cagaacaaag tcgtaggtat cctgactcgt gcatcattag tggatatcgt ctacgatgtg
     8401 atttggggag aagatcccgc aactactgat gttgcagagt cagctactcc tgaagaatct
     8461 aaggaggtct gacctatgca gcagtttttt catcagcacg gttcagaaat tctctcaaaa
     8521 ggttttgaac atctttatat ttcaggactt gctttgatca ttggtatcat cattgctgta
     8581 ccgcttggtg tcttactgac acgttttcca aaagctgcca cgattgtgat tggcttaacc
     8641 agtgccttgc agacaatccc ttctcttgct ttattggcat tgatgatccc gctatttggc
     8701 gtaggtaaat tacctgcgat tatcgcactc tttatttatt cattattgcc gattttacgt
     8761 aatacgtaca ttgggatgaa aaatgtcgat tggaattatc gggatgtggc aaaaggcatg
     8821 gggatgactg agttccaatc cattttttcc gttgaattgc ccattgctat gccaacgatc
     8881 atggctggta ttcgtttagc tgccgtttac gtgattgcat gggctacact tgcctcttat
     8941 atcggcgctg gtggtttagg cgatttgatt tttagtggac tgaataatta ccaaccagat
     9001 ctcattttgg caggtacgat ccctgtaaca attttagcac tattggctga ttggcttcta
     9061 ggtctcttgg aacaccggct gacaccgatc gcacttagag aggagaatga agaatgaaac
     9121 gtatactaaa aatgaccatg gttcttttct ctcttcttat acttgcgagt tgttccttcc
     9181 ctggtcttgc cagcaataca gatgaagaca ccatttcgat cacaggtgga atcacttcag
     9241 aagcacagat cttagcaagt ctagttgccg gaatggttga acattataca gataaaaata
     9301 ccgcaatcat caacaatctg gctactacaa ctatcaacca tcaagcgatg atgaacggag
     9361 atgcttcaat ttcagcggct cgctacacag gaacagattt aacgactaca ttgaaccttc
     9421 ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa agatgaattt gagaaaagat
     9481 acggtcaaac gtggttcccg tcctatagtt ttgaaaatac gtatgtcttt cttgttcgaa
     9541 aagacaccgc acaaaaatac catctatcta aagtcagcga tctgaaaaat gtagcggatg
     9601 aattagtagc tggtgtagat acttcttgga tcaatcgtaa aggagacggt tatgacggct
     9661 tccaagaaac ttatggcttc tccttcaatt cgatattacc tatgcagatt ggacttgttt
     9721 atgacgcagt cgaagcagga aaaatggata ttgttcttgg ctactctacg gatggacgga
     9781 tcgccagtta tgatctagtc atgttggaag atgatcgtca attcttccca ccatatgatg
     9841 ctgctccagt tgtagacaac aagatattag ctgatacgcc tcatttaaaa gaagccttaa
     9901 ataagttagg tcataccatc agtacggaaa aaatgcagga attgaactac gaagcagata
     9961 ataatctagt ggaaccatca gtagtagcac aacgtttctt acaagaaaac cactattttg
    10021 aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa caatttttct attatttcca
    10081 ggaaaatggc tcctatatct ttgcacaatt tatccgtcac ttcctgattt ctatctatgg
    10141 tgtcctcttt gcagcagttg tcgggattcc ggttgggatc atgatttcta ggagacgtaa
    10201 attagcaaac tgggtgattc gtttggcgaa tatcatccag acgatccctt ctctagccat
    10261 gatctccatt ttaatcattg gattaggcct gggagtcaat gtcgtgattg ttactgtatt
    10321 tttgtattcc ctcttaccga tcatcaagaa tacgtatacc gggatgatcc aagtagacaa
    10381 aaacatcctt gatgtcggca aaggcatggg gatgactgct agacaacgac tattcatggt
    10441 agagttacca ctctctgttt ctgttatcat ggcaggcata agaaacgcgt tagttgttgc
    10501 catcgggata accgcgatcg gtgcattcgt cggtgctggt ggtcttggag atatcattat
    10561 ccgaggaacc aatgcaacag agggcacatc gattatttta gcaggtgcat tgccaactgc
    10621 attaatggcg atcatcacgg actggctttt aggtattttg gaaagacggc tagatccagc
    10681 cagccgtaca tcacgctgat gttattgtaa aaaacaaaag cttctgcgac catttgttgg
    10741 tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata atccactctg attcttccct
    10801 ccttatgggc aaagagttgc ttttcttagc tatttatcca ttccgtatac tccaacttca
    10861 tactgccaac gggttcatat ctgcatccac cgactaagtt tttttcttat ccgctaaggc
    10921 attttgccaa tccattttcc catcactcgt catggaattc ataccacatg atctctaaat
    10981 actcgtctgc ttgttctttg aattgggcat caattcttac atcctttggc gtggctttca
    11041 tttaagcctt ttccatcagt cctcgattgt tcgatgcctt cagctgcatc atcaaaactg
    11101 gtgactgccg tatcggtaac ttcgatcatc ttttatacaa tcgggaactt gccatcctca
    11161 tccttggcta aaaacatatc tagccattcc agcttctcgc catgggaggt ttgcggttcc
    11221 taaaaccggt acacgattct tatggactgc atccgtcgca actagacttg gcgatacgat
    11281 gatccttcac cagaagagca cccccgtaaa ccaattgatc gatatattgc caataagaaa
    11341 agacattggc gtaaaagata tcgatccctc gtgatgcatt gccgttgata ctgctattca
    11401 tggccgaaag cgccacgact ttaacttctt ccgtttgagt gacattcatg ttcggcaatg
    11461 tcgctttgga tacccgctct gctaaaggaa cgacactggt attgtatttc gtatctgtat
    11521 cttttccaaa agaccatgcc aacgaatcct ctgggaacca ataagacgac tccggttgat
    11581 tgtccattgc ttttttcaac atacttttgg ctgtttctcg ataaacgaca tttccaattg
    11641 tcttcgcctc actttggcga taatcgatgg tcgttgccac tgccaacact aacaacgatg
    11701 ctccaatttt actgattttc cttgtcacac ttcttcctcc ttgttttaca taaacccgtc
    11761 aacattccat tcctaaactc aaacaatgac ccttcaaagg tggagatata ggaacaaatt
    11821 aaagaaatca tgcaataaat ccaagaaggt tggttttaga acaaaaaaag agtctccttt
    11881 ttggggagac tctttgttaa ctatctaaaa aggttggctc ttctcacttc actcataatg
    11941 tgtccaggca gaataaattt ctacaacttt attttcctca tcaactttgt aaaccactcg
    12001 atgctgtcta ttcagcctgc gtgagtatct gccttcatgt gtaggtctca gcttttcaaa
    12061 agactgcgtg ggcatatatg gatcttcctt caatgtctga atcacttctt caaattgttt
    12121 ctttaaattc gtttgcttga tttttcttaa atccactttc gcagagtttt tgattgcgac
    12181 tgtatagtta ctcatcaaag agcttcccaa tcaatatcat cgatattcgt aaagctactg
    12241 tcgtcttttt cgcggtctcg tactttatct aaagtacctg tttgctcaag aaaaagggtt
    12301 tcttgaatcg ctctccagtc ttccagccca atcaatacag cattgtttcc actacgatca
    12361 ctaataatct cgatttcagt atgattttca tttacctctt tcaataactg ataaaaattt
    12421 ttacgagcag tgctaggatt catcgttgta ttagccattg tctctcctcc tttttctgat
    12481 ataattatag cgtacttgat taagtacgtc aaaaaaatac aataaagcac ttaaattcag
    12541 caaacttttc ctctttttta catagagaaa aagacccctt tttagggagg atctttgtcc
    12601 acgctcactt cttactcctt tacgactagc ggtagccaaa cgtaaagtaa ttggtaacaa
    12661 cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag tctatgcaac aacaccaata
    12721 cacgaaggtt cctcgttgca gtgtgacttg attgatatct tgaataacct gaggatacgc
    12781 acgaaagatt tgtaacggtt attcaaaact gattttggcc gtcaagtctt ctactgcctc
    12841 aaaaatgatt gcctctttcg tctcctgtca ttcatgttca ctatcgattt gatacttgct
    12901 tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac tgagttatgg taacaaaaaa
    12961 tggtcacttg acgaggctga gattcctact tatgaagaca catggcactt aagagtcgag
    13021 gtaaaggaag aagctgaaaa tataggaggc tgtacattat ttggcaaagg attcggaaat
    13081 tatgatcaag acatcaaaat caaaatgttt tacagctaaa taggaagaat aataacattt
    13141 catacgctgt tcccatcttt aatggcttat agagggcacc tttagctgag aataagacaa
    13201 aacagaccct ctagcttcaa ttaagagcta gagggtctgt ttttgatttt aaaacttggt
    13261 ttatttcttc aaatggtagc tataggtgct gacaacgata ttttcacgcc aagacaatcg
    13321 caatcttaac cgcaaacttg tttggttatg gagaatattc tgccagcgtc tgtttggtac
    13381 aaattgagga atcaacacag tagttgtata gttttgtttc gctgcatttt tacttacgat
    13441 atctacataa cggataattg gattttcaat cgagcgataa gaagagtgga cgattgaaaa
    13501 acggacatct gggaaatgct ttttaaattc tgcttcgatt tctttttctt tttcgacatc
    13561 ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg atcgaacgtg cgtaattcaa
    13621 cgcaccgata tttacccgtg tcacattccc gactaataca agaacggtat tcccgtcata
    13681 atcatgtagt tttgcatcat tttctaaacg aagctgttct gctacttttt tataatgatc
    13741 atggatctta taaaaaatga acataacgat tggcataatg atgaagaaag gccaaatgtc
    13801 ccctaaccga tagacaaaca aaatcgcaat gattgcatat gagatgaacg cacccgtaat
    13861 attggcaatc gatttgctta accatctttt tccttctttt ttccatttga cgaccatccc
    13921 tgtttgagat aaagcaaatg gaatgaacac gccgatagag tataacggaa tcaagcgttc
    13981 tgttgagcca tggaaaataa acagcaatac gatcgaacct agcgctagtg tgataatccc
    14041 attggaatag cctagtcgat ccccacgatc ttgatacatg tgaggcatga atttatcttt
    14101 tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg gtgttagcag caactgctaa
    14161 aatcagcgct gtagcaaatt gaaggacgta atacaaaata ccatgtccaa agacagcttt
    14221 accaatttga gacaaaacag tgacttcttt ttcaggaacg atcccatacc aatagttgat
    14281 aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc atcatcgtca atgtagcagc
    14341 ggcatttttt gctcttggct tcttaaagaa tggtacagca ttactaatcg cctccacccc
    14401 agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc aaagcgatag aaacacccgg
    14461 cacagccgct cctggaagtg cagtagcttg caatggcaca gcacctgtca cgatattata
    14521 taaaccaaca acgatcagta acgagatcac aaggataaat gtgtacaccg gaaataatag
    14581 gaacgatgca gattccctca gcccacgaag attcagcatc atcagtagca aaacgattgt
    14641 cacagaaata gcgacttgat gaccgtagag tgctggcacc gctgatgtga tcgcttcagc
    14701 acctgctgag acagatactg ctactgtcaa catgtagtcg atcaataaag agccaccgga
    14761 aatcaagcca gcattctttc caagattctc actgctgacg acatatgctc ccccgccatg
    14821 aggataagca tggatgattt gacgatatga tagcgttaat gaaatcaata aaataataac
    14881 aaaagcggca atgggtagcg aataccagat ggctgcagca gacaaagcga ctaaaacgac
    14941 aacgatttgt tccgtaccat atgcaataga tgataacgca tcagacgaca ataatgccaa
    15001 agcagcaaat cgggttaatt tgtgctcatc attttcagca gattttaaag gcttacctac
    15061 taacaacctt tttaagtaat ccaatttcta aactccttta cacacaattt tacttcaagt
    15121 tttatagtcg aaaatttata gtaaacaaga tgttatagta cgctcactga ccttaaaagt
    15181 caataaactt taagaaaata attggaaaaa aacagaaaaa tatcaagcgc tttttcaaac
    15241 gctttaatac agcaaaaatc taacattttt ttatgggctt tttctttgaa ttctataata
    15301 aaaggataga acttgactta gaaaatcaag ttctatcctt tttgatatct taacttttgg
    15361 aagagagatg taaggctgct ttctacccat cattcttact actcgaagca tagcgcagtt
    15421 gtcacaacct cttgataaac ggtgtttaaa agcttgcatc tgcggtaata agcccaacca
    15481 aattaaaaac ataagaagct gtttttattt ggtcgttctt attacttgat gcagagcagc
    15541 ttttttacaa cctcttggta cacggtgttc aatcagctga ccctcggtat tagctcaaaa
    15601 aaacggcaaa atatgaggaa ctatttttcc atttttttct ctaatactca ggtcaaaaca
    15661 ctgatttcac aacctctttt tttaatgaaa aatatggctc tcatgcgccg cttctttgaa
    15721 tttcgtcatt ttttcttcga tgaacggata gattgccgtt cctacgattc caaagacaac
    15781 aaacaaaccg atcatgatcc agattgcttt cgtagcattt ggccaataaa ttcctcctgc
    15841 agattcccgt aacaagttta ccgcatgagt aaatggcaag aacggattga tcatttggaa
    15901 gaatttccct gagacttgga tcggatagtt cccgcctccg ccagagatcg acagtactaa
    15961 gatgataata gcgatccctt taccgacatt atcgaataat gcagccaaga catagacgat
    16021 catcatgaac gctaaggcga tcaagacagc aaatgacacg ctgtagaccg gattttggac
    16081 atctactcct agtaagaaaa tattccctaa tgtaacgatc aagctttgcg caatcgccat
    16141 tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt ttcgtaaaat tgtttcggtc
    16201 tttctttcct aaatagtatt ctgtcgtagc aacacttgaa agaagaacag cgccgaccca
    16261 cagacatagt gcagtataaa acggggtgct agctgaaccg ttatttgcga ttggataaag
    16321 cgcattggtt tgtagctcaa caggttgtgt aaagaagtca ctttcttttg ttgcgtctag
    16381 tttcaataat ttgatgactt cgcctagatc gacttcttgt tcgccttttc ggatcgcgtc
    16441 tgctgctttt tggataccag ctttgatatt tggccagtca ttgttgatca agtccgcagc
    16501 agcgttcaca gctgtttcga cttctggcat tttttcattt actgttgtca atgtaccggt
    16561 gatttcattt tttagtgctg gccaatcatt ttggataaaa tcagcggcca atcctaattt
    16621 ttgctgaatg atcggtaatt cattgttgta taggtctgcc ccttcattga tgccattgac
    16681 gatggtatcc atatttccat tcagcattgt attggcatca tggatttctt gtccaattgc
    16741 aggcatttct ttttgatatt tttctaaaat tgccactgca ttcgttacag ttgctttcgt
    16801 tgagctgaga agcgaatcaa agtcgatctg gtccgctttg ctcagtgcgc cttgtgcagt
    16861 cccaatcgta tcgatgactt ggtctagtag agaagtgaca gtggaactga tttgacctgt
    16921 attgatacca gcaatgatgc cattgatatt attaacaacg gcatcaattt gatctagcaa
    16981 ttcatcaata gccttcgtgt cttcattttg aacagctgtg ttcaaagcag ataggcggtt
    17041 ttgcagattg cttagcaagg gttttaattg atttagctga tcaataattg gctgtaattt
    17101 cgtatttcca gcacttgttt gcaatttagt caggaactcg accagtttat cgattgcatt
    17161 ttgttgttga gatattccgt caataaagcc gttcaataca ttgctgatgt ttttcttttc
    17221 atcatcagtc agcttaccat cttcaatagc tgttcttaca gtatctatta acgaacctgt
    17281 cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct acactgtttg tgatgcttgg
    17341 tatcgcatct ttcagctgag aggcacctga ttttgtttgg tttgctagat cacttgcctg
    17401 attttctaaa tttttcacat ctggcaagat atcttgtact tgttggatga tcgtcaagcc
    17461 ttgttttgct tcatcgattc cttggttcat tgtctcttgg atcgaagcaa aatcgccgtc
    17521 gatctctgct agttgcttac cggcattttg gatctctggg attttttctt gcaaatcaag
    17581 gatgattttt gcttgatctt tcaactcagg cattttgtca ttcaatgcaa cgactttttc
    17641 acccatctca tctactttag ggatgtaatc cacgaattca tttgctttat tcagtttttc
    17701 cttgatttca ggtagttgac tttgtaattc gaggacttgt tttgtgtagc cgtcgatcgt
    17761 gtccaaattt tcatctgttg ataaaatcat atctttaact ttattgatac ttactaggtt
    17821 cgtatcgatg tcgtaaccta tctcattgaa tactttcaat aaagtcgaac tggctgtttt
    17881 gataaattca tttgtgattt gcgattggat cgaacttgct cctttatcgg tgattttcgg
    17941 tgcaatcgca ttgatttttt catttaccgt atattcgatt tttggtttct tgatatcacc
    18001 actagtaaaa ctcagcagat cttctgagaa atcctttggc aagtaaatac ctgcgtaata
    18061 ctttccggaa cggactccgt cttctaattg ttcttttgaa tcaacgaatt gccatcctaa
    18121 ttgtttattt ttatgaagcg attcgatgac ttgctcccca atggccactt ctttaccttg
    18181 aaactctgct ggcttgtctg cactgtaaac agcaattggc agctctccag tatttccata
    18241 tggatcccat aatgctttga tgttaaacca cgcatatagc gaagggatga tcatgatcgc
    18301 gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt ttccagtcaa gcaaatataa
    18361 tcgaagcgta tttttgattg actgcatagt tctccccctt cttctttttt cagctgaatt
    18421 actttaccat gtatttcata cttttttcaa tcggcttttt tcaaaaaagt cgaatttttg
    18481 gacatatcac ggcaattgtc tcattgtaat ctcttacata ttgagaaata attaaggatt
    18541 acagccttta ctttatcatg aaataaaagg gaaagataat tttatgccaa agtttttatc
    18601 tattatacgt atacgcaaaa aagctaaaac attttttgtc tttgccccta caaaaatgtt
    18661 tcagcttttt tcgtttttta ttcctgtatt tcttattttt cgaatgacag gatgattttc
    18721 ccagcaggag gatgtccttc aactagctga tgtgcttgga tgacatgctt aagtgaagca
    18781 gacagattca ttgaaataaa aacatgaaag gctccttttt ggtaggcttt gattatgcca
    18841 gcaaaggctt ctgcatctag atattccttt cttggaacaa acgattcata aaagccttcc
    18901 tttttttgcc gcaaatctag atctgggagg tcatttaatg caacatacct gccgcctggt
    18961 ttcatgatct ggatgccggt ttctcctttg atacttcctt tcgtcgcatc gattacaagg
    19021 tctgcctgat cagcaaattg cagacctgga tttgttttat cataagcagc aaatgctgaa
    19081 gctcctaatt tttttacctt ttcctcattt ttactggatg cactggttaa gattcggatt
    19141 cccttttcat ggagaagctg gatcagactt gacccgaccg cacctgatgc tcctagtatc
    19201 attacagtat ctgtcggctg gatttccgtc aaatggttga ttagattata agccgtaatt
    19261 cctggcgtga ccattccggc tgcttcttcc caactcattt tatctggaat ttttgctact
    19321 tttgctgatg gcaacacgac ttcttctccg taagtcccgc cgactgcatg gacagctact
    19381 cgatcaccta catgaacagt tgtgacatct gaaccaatct ctgtcacaat accagcgcca
    19441 tcattacctg gtacataagg gaatttcaat gttcgtatct ctttcatttc tcctaaacgc
    19501 aaggcaacat cataaggatt cacactgaat gcttttattt ctacacgaac atgtccagaa
    19561 ccgacttctc tgggatgagc atcgatttct tcaaaaacat cttgtgcttc tccgtaaccg
    19621 ttaattgcaa atcttcgcat ggtcaccatc tccctttttc tttaagttta cgtttatgcc
    19681 tttattttga caaaaaaaat gtcttttcgt tttggcgcat gattcaaaac aaaatagttg
    19741 tcttttttcg cgtaacgtgt taaattattt atctaaaagc gtaaagatta cgatttacgc
    19801 taataaaaaa taattggagg aatttgttga tggctaaaaa atcaaagatt gccaaagcaa
    19861 aaaaacagca agaaacgatt gaacgctatg ctgctttacg ccaggaatta aaagcgcaaa
    19921 aagattacga aggcttgcgt aaacttcctt tagatgctcg tccagaacga ttgaaaaatc
    19981 gtgatttaat cgatggacgc cctcgcggat acatgcgtaa attcgggatg tcccgtataa
    20041 aattccgtga attagcacat aaaggtttga ttccaggagt aaaaaaagcc agctggtaat
    20101 ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac cactccttgg atcatcaaaa
    20161 aagatacgtt cgcttcttct agcagaaatg ctggtactag aagcagacgt atcttttttt
    20221 acgcacagct ggtccttttt tatatgtaaa ggctgcatgg ttacttcgct atatttattt
    20281 agtaacagga gtcagtgctt cgatacccac actaattttc aatgctcggt caacttctgc
    20341 cataatgggt cgatccaaat ggcagacctt ttctttcaat cttgatttat cgatcgttct
    20401 gatctgctcc aacaggataa cggagtctct ttctattcct gttccagtag ctttgatccc
    20461 aatgtgagtc ggaagcttag gtttagccat ttttgccgtt atggccgcaa caatgatcgt
    20521 cgggctaaaa tggttaccta gattattttg aataaccaaa actgggcgtg ttccgccttg
    20581 ctccgatccc acgactggag aaagatcagc aaaataaata tctcctcttt ttaccatagg
    20641 atcctcctta ttcttgatat tctcttggta tccgctgtcc taatccgcaa gccacttcat
    20701 agtggatcgt acctaattga tctgctatat cctgcatagt gatttctttg tctgcatttt
    20761 tgccgataag cgtcactttt gttccgacag gaaattcttg aggcaatcgg atcatcagtt
    20821 gatccataca tacacgccca atcgtttcac agtagttccc ttctaccaat agagaaaaac
    20881 cctgcatctt tcgtggccag ccatctgcat aaccgatcgg tatcgttccg atccactctg
    20941 cttcaggagt gatatatgtt tctccatagc cgattccttc acctgctggg agttttttga
    21001 cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa tggataaact tccggtagtg
    21061 cgtgcccaga gggattaagt ccgtacattg ctactccata acgaatcata ttgccgattg
    21121 tttcatcatg ccataaagcc gttgcactat tgctgacatg tacatatcga ggaagttcgg
    21181 gaagtttttt cagcacttct ataaagcgtt ctttttgcag attccaatat gtatcatctg
    21241 cctgatctgc tgtagaaaaa tgggtaaata aaccttccca ttctaataca cgactttctt
    21301 tgatgaatgc agctgcctct tttacttctt caggtgtaca aaatccgata cgacccatcc
    21361 cagtgtctgc ttttagatga atcgaaagag gcgtctctag cttacttttc tctaaaacag
    21421 cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac tgataaatca tgagcaacag
    21481 ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag gatgggctga gtaaatccag
    21541 cttctctcaa ctcgactcct tcatctaaat ttgaaacaca aaagcccgaa gctccacctg
    21601 caactgctgc ctcagctgtt tcaatagctc catgaccgta tccgttcgct tttactactg
    21661 caaacaattc ttttccttgc ggaagtcttg caacttcatt tgctacattt tcagttatag
    21721 cttttttatg tatcactgct ttcgttgggc ggtgccaagc gacaaccatc gtttttcctt
    21781 ctttctagtc ttccaaaata atttgtgcaa aagctgtttc atttgtatga gtaatactga
    21841 cccatacatt gccttcatga ggtgaacgcg tcacaacagg ctgaccattt tcatttttta
    21901 aaatctcgac atcttgaaag gtaacttttc cgatcccagt tccccaagct ttagagaaag
    21961 cctctttgca agcgtaacgc ccgcctaaaa attctacttg gcgatgaaaa ggcaaggact
    22021 ggaatagttt catttcatca gaggtcaaga ttcgtgcaat gaattttggt ttttcttcga
    22081 tcagccgagt gatcctagga agttcgactg catcgatccc aattcctttt atcatatgct
    22141 tttctctttt caataaattt agaacactct cattctacca aattataacc attttgccac
    22201 aaataagtcc acaaataaca aaaatgaaag tttctcttaa aaaagagaaa ctttcatttt
    22261 atgtttaatc gttatttgaa cggattacaa atccgcgttt tttatcattg tgacgtttac
    22321 cgccatcttt ttggctgtta ttatttttgc tataaccgcc ttttgatttg tttccacgat
    22381 aattaccgcc gtcacgacga ttacggttgt ttccgccacc acgtttgcca tttttattgt
    22441 atcctttttt ctgcattggc aatgggcgtt ctggtgtgat cttcactggc actgcatcag
    22501 ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa atcttgtgca gaatattctt
    22561 ctaataattt atctgctgtt tgcaggtatt tatctagacc attttctgca agttttgttt
    22621 cgatttgttc aactgcagca cctaattgac ctttgaatgc ttctttttcg gttggtggac
    22681 gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac atgcaagtag tccatttcat
    22741 ttggtgtaac gaaagtcaca gacataccgc cttttccggc acggcctgta cgtccgatac
    22801 ggtgaacgta gctttctgga tcttgaggga tatcatagtt gtacacatga gtgacacctg
    22861 aaatatctaa tccacgtgca gcaacgtctg tcgctacaag gatatctaaa tgaccgctct
    22921 taaatgaacg caatacgctc attcgttttt gttgagacaa atcgccatgg attccttctg
    22981 cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc tacacggcgt tttgtacgtc
    23041 cgaagacgat tgttaattca ggtgtttgaa catcaaacaa gcgagtcatg atatcgaatt
    23101 tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa gtcagctgtc atttctttcg
    23161 ctttgatctt cacatgatgt ggatttttca tgaatttaac gccaatattc ttgatagctg
    23221 gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc aggaacttgt gaaatgattt
    23281 tttcgatatc ttccaagaag cccatattca acatttcatc tgcttcatcc aaaactaatg
    23341 tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg gtcaagcata cgtcctggtg
    23401 tacccacaac gatatgtgga cgatctttca agccacggat ctgacggcca atatctgctc
    23461 cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc taaacgatat aattcttctt
    23521 gtgtttggat agctaattca cgtgttggtg cgataactaa accttgtaat tcgtgacgat
    23581 ctggatcgat tttttctaac attggtaatc caaatgcagc tgttttccct gtacctgtct
    23641 gtgcttgtcc aataacgtct tttccagcta atgctagtgg aatcgtttct gcttggatag
    23701 gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa caattctggt gataattcta
    23761 gttctttaaa tttcaaatga atcctcctat atggttgtta actgaggatg tagctttttt
    23821 gctaccccgt cgatttgttc ggtcaaagtg tattgtttct ttttgacagt ggccacgcac
    23881 gtttttatcc attcttagcc cctgtggcac caccgaaaaa tcactttgcg aagtttaggc
    23941 attagaaaag ccaaacgggt cgcgttcagc tttatcatta ttatgacatc tggcttcaac
    24001 aagttgtttc tatggcaaaa ccagtccaat ttctcttcat ctttcggcaa taggttattc
    24061 taacatagtt tttcgttttc tgcaagttta cagattttta tttcatttgc tgaagttgtt
    24121 ctaccacttc aaataagccc atgctgttgc tgccttttag cataatacta tctgttgctt
    24181 caagattttg tttgatagca tcaatcaatt tattcttttc tttttcaaaa tagaacacag
    24241 gtagttgggg gtatttttgg ttcaattctt cttttagtgc atgcatttgt tccccataaa
    24301 ggaaaatcat ggcgaatttc tcaggatcga tatgttcact catttgcgca tgcatattta
    24361 aagactcttt ccctaattcc agcatatctg ctaacacagc gattctgcgt ccttttgttg
    24421 gcagattttt aaaagtatct aataccagtc ccatggctgt tggatttgca ttgtagacat
    24481 cgctcaaaat agctgcacca tttcctgccg ttagccattc ggttctgttt tgagtgatag
    24541 aaacatgggc gagcccttgg cgtatctcag gtatggacag tccaaagaag cgcccgatcg
    24601 tatacgcaat caaagcattc gttacattat aacttccagg cagcgggatc gtaaatgttt
    24661 ctccttctac tacaaaatcg gtttgtgctt ttccttttgc tgtcacttcc gcatgaacat
    24721 cgccatttcc aataccgaat gttgtgactg tttgtactaa tgttgccgtt aaaggtgtca
    24781 gcaacggttc atctgcgggc acaacgagta atccgtctga tacaagtccg tcagtgattt
    24841 ccattttggc tttagcgatt ccttctcgcg acccaagatt ttctatgtgt gcttcaccga
    24901 tcattgtgat tgctgcagct tttggttgag ccataagtga aagttccgtg atttcattgg
    24961 cgtgatccat gcccatttcc aaaacgatct tttctgtatc ttggggcatg tgcaaaatcg
    25021 tgtaaggaag accaatatta ttgttgtaat ttccttgcgt cttatatgtt ttgtattttt
    25081 cagacaagac agacgcggtt agatctttcg tggtggtttt accattgctt cctgtaattg
    25141 caacgacttc tgcgcctact ttttccaagt aataggttgc caattgctgc atggctttca
    25201 aaggatctgc tacaggaata actggaaaat tttttggagc ttgttcaaca ggcaagctcc
    25261 aaaatgaagc agatgctccg tttgtgatcg cttgctggat aaattcatgt ccatctcgcg
    25321 taccagcaag aggaacgaat aaacttcctt cggttatctt ccggctgtca aattcgacat
    25381 tggtaatctt tgtcagtgca gagcattcgc tgcccacggc ttgtgcaatt tcttgtattg
    25441 ttagattcat tgagatctct cctaaaaaag gttgtgaaaa aagcgtttgg cctaaacggc
    25501 gggttcgcat gactctcact tccaagtaaa tttctctatt cgtatttaga agctagaatg
    25561 aacgcggtta cgcaatcact ccagcctctt ctttatttct ctttattcat ttttttcaaa
    25621 aaaagattga cgctgatggt atcgattcat tcctaactgg atcagttctt caatcaaatc
    25681 accgtatttc aagcccatat tttcccataa gagtgggtac atactgaact ccgtaaatcc
    25741 tggcatagag tttaattcat tcaggaataa ttcattttta tttgtcaaaa agaaatcgca
    25801 ccggctcaat ccgcttccac ctaacatcgt gtaagctaac ttcgcgtact cttgcgcttt
    25861 ttgataaact tcttctggca cttcggctgg aatctgcatt tcgattttat tattgatata
    25921 ttttgcttca taatcataga atgctacgtc ttttacgact tcaccaggca aagtcgtccg
    25981 aacgtcttca tttcctaata cagcaacttc gatttcgcgc gcttcaattc cttgttcaac
    26041 gattgctcga gaatcatact gataggctgt tgctaaagca ttttgcagct cttctcggtt
    26101 ttctgctttt gtaatgccga cactagaacc catattcgcc ggtttgacaa acatcggata
    26161 aagcaaagaa ccttcacatt gatcaaatac ttttttagga ttttctttcc attgattctt
    26221 aagtactggt acataaggaa cttgcggcac accagcagct tgtaaaatat acttggtcat
    26281 gattttatcc atggcacatg cactggtcaa tacgcctgcg ccgacataag gcatattcaa
    26341 tgtctctaag aagccttgga tcgttccatc ttccccgttt ggcccatgta aaactggaaa
    26401 aacgatggct ccttcttctt tgatttcgcc cggattgatc acttttcctg taaagccttc
    26461 ctctgtctgt ccacttgggt cccatgaaag atgcaagaca tctttgctgg caggtttttc
    26521 tgttagtaat ggacctttga cccattgtcc ttcttttgta ataaatacga gttgaacttg
    26581 gtaataatta taataaatgg catttaaaac tgaaaatgcg gaaagaatgg acacttcatg
    26641 ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa agattgtcct cctgtattct
    26701 aaatcgttca gtgatttact ctcatatttt agcacaaaac aaaaataaac gaataactta
    26761 tttataaaaa aacgagccag cttctaaaag aacgagcgtc atttattttt tgtcagctag
    26821 attatttgtc ccgtttttta taaaaagtta tgataaagaa aaggagggat gatgatggcg
    26881 caaacaaatc cacgcagccg cttggcagaa atcattcgag tgttcatcaa ttacaatgta
    26941 gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa aaaaagcctt tgaagaacta
    27001 ggccccacct tcatcaagat aggacagatg ctttctgttc gagaagattt gctttcttct
    27061 gccttcactc aaacattcaa aacattacaa gatagtgtac ctagcgacac attttcaacg
    27121 gtcaaaaaga cgatcgaaac agaactttct ctttcattat cagatatatt cgatgatttt
    27181 tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc atcgtgccaa gctgaagaac
    27241 ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg ccgaagagat ccgacttgat
    27301 cttcaactat ttgaacgagc gatccctttg atcaaataca ttcctgaaac tagcgtagtt
    27361 gatctaaaag gggtactcca agaagtaaaa cgttccttga tcaatgaaat ggacttctta
    27421 aaagaaagtc aaaatggcga acaattctat caaaagaaca atggctggaa agagatccgc
    27481 agccccaaga tttatgacgc tttttgttcg aaaaaagtga tcgtgatgga agaaatgtct
    27541 ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa ctgaaacttt tatcactgga
    27601 attcaaaaca agcagctgaa acaagaagtt gcaaagcttt tagttgagaa ctttatgaaa
    27661 caggtgtttg atgatggttt tttccatgct gatcctcatc cagggaatct gctattccat
    27721 gtactaacaa aagaagaaca gacacaagct agccgaaaaa ctgaaacagt ccatgaaaaa
    27781 gagttcggtt cctttgcttt tcgagcctcc acttctgcag aagaccctgt tgcgccatat
    27841 accatcaact acatcgattt tggtatgatg gggcatcttt ctgcaggact taggcaaaaa
    27901 ctcacacaag ccgttttagc gctgtatacg aaagatgctt atcgaatcga aaaagcggtc
    27961 cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc ggttccacca agaactgaca
    28021 agttttcttg aacagtatta tgattctcct attgatgaaa tcaacttgca agaagtattc
    28081 acacatgtcg tgacgatttg tcatcaaaat aatctgcagt tcgatcgtga tatcacgctt
    28141 ttgctgaaag catttgggac attagaaggg gtgatccgcg tcctcgatcc tgaagtttcc
    28201 ctcatggaag tcgcttcgcc ttttgctcaa cattattttc tcactcatct ggatgtagaa
    28261 gatacattga aacaaagcgg attagactta ttggaaggga tgaaggctgc gcctaagatc
    28321 cctcaacagc tccaccatct attagaaatg tggacttcgg gacaaggaaa agtcaatttg
    28381 gaattgaaaa agcaagacaa attattgagc aggatcgaaa gtatgatcaa tcggcttgtt
    28441 ttcggtatga ttttagctgc attgatcgta gggtcctccc tgcttgtcca agcagcacct
    28501 gtagaaaacg cagaggtcgt ttccttatta gggatattta cgtatgctat tgccgcattc
    28561 gtcattattt ttttggcaat cgatgcattg atccaaatgt ataagaagag gaaaaagtag
    28621 aaaaaggttg tgacagaaag aatcagcttc aagaaataag aaggggtttt acataaattg
    28681 tttctcaaat ttttgtgaaa attcggctta tttttcgaag aggctacttc tgaaacaccg
    28741 agaataaaaa atgtgaaaaa gctgttctgc acccagtaat aagaacaatc aaaaagggaa
    28801 catggaacac atttatcctg tttccctgtt ccctttttga tgtattcgag cataaataaa
    28861 ataccttcgt tacctactat cttctgtact tggtaaacct tagtcaattg ccttttttaa
    28921 cgcttgacga aaagacatat gatctttctc cgtaaagggt tttggaccct ttgtccttat
    28981 gccagctttt cttaactttc cactatgata acgttgttct agaagcgtgc cgatattcgc
    29041 ttcgtcaaat tcttttccag agtgatggaa cactcgcaca cccttcggca atacaagtga
    29101 agctaaacca taatcttgcg tgatcaaaaa gtcattcttc ttaaccatac cgacgatttt
    29161 ataatccgca ctatcagctc cgcggtctac gtagacgatt tggacatgat tagcgtattc
    29221 tttggtcgta taatggtcca cactggtcac gataaccacg tcaagaccat aggattctcc
    29281 taattttatg acatcttctt ttactggaga accatctcca tcaatgaata gcctcattct
    29341 ttttcgcctt cttccataaa tgataaacag ttactaatcc gctcagtaaa agaccaacat
    29401 tgatccattt tgccaagata agcagttgta cactttctat cggcactgat tcatggttca
    29461 gcgtgatcaa taatagatgg attggctctc ccatttcttt tgctaaaaca aacaaaagcg
    29521 tcatcaataa aaactggtgg atcacactcc ctttattttg agtagtatac ttgacaacga
    29581 tccggcgatc cattggaata gctgtatcag tttgataata ttccttattt agccagctgc
    29641 ctgttactac gtgccagaaa ctaagcagaa agacacctat cgcaaaacca tttggaatca
    29701 agaggaccaa taaagaagaa ctcagtccaa gcaattgcat agccaatgga gaccatgtta
    29761 aacgtttcaa aatccatggt ccgactattt ttgctgcaat caagcctgct gcataaggca
    29821 aatacataaa aatcgaaagt ttttctaatc cgtacagtat gccaacataa aaactgctaa
    29881 ataaaaaaag gaaattccct agcattccat ttagaaaagt aaacagattt agccagcttg
    29941 gcaacttcca ttccttcttc tggtgtccta aaacaaacac gaacaagatg aacagcagca
    30001 agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa taacaatcgg gcatttctca
    30061 aaagaaacaa taagccaaag aatatcaaaa ataaaacaag ctctcgcgta gctaccagat
    30121 ttcttttgat gtctttgaaa tccagttcgt aacgcggata atgactaacc gtatggtagg
    30181 ccatgacgta aaaaagtgta taaatagtaa agcttgctgc aatctgagtc ggtgcaggca
    30241 actcaatcgc ttgatacagg agaaatagcc agatagctgc aaataagtac tttttcccat
    30301 ccatattgat aaaaccttgc gtcttttcgt gaaaattgac cgtggtatta gcaggtacaa
    30361 gccatgctgc actcagccct agcaaaacac ttgaaaaata aaataaagga aaataaaata
    30421 cgcctaatat tccaagcaat gaaccagcac caccaatcag cagagcgatc atcagcaacg
    30481 tgtaactatc tagaccaaat cgtagactgt tgactaaaaa tagtccagtc atccgaaagg
    30541 cataaaacag cgtaaatggc aaaatcgtca gccaattatg cgagtctgtt agtcccaaaa
    30601 ataaaagata aggaataaaa ataaccgtgt tacttaagat aaagggcgtt gtttgtgtaa
    30661 aattacgccc aaatcgtttg agtttttcga acatgacaat ggctccttaa acagtcttat
    30721 aactaatctg ctcttcgatt ttagcattaa aggggaaata aaccaatttt acagaatata
    30781 ttcttgtaaa aaaaaataca gaaagaagca taagcttctt tctgcatttt tacggtactt
    30841 gattactcaa gcagacaagc ccttctccac aaaagtcttg cccgacaccc ctgcatccgg
    30901 caggtgataa agatagaaat caattatcct gtgcctccaa aatcaattag gagctctgta
    30961 aaccagagtc cttctcgacc cgtcgcataa atttacttta acacagttcc tatgtttatg
    31021 cgagagcttt tttcatttta taatcgacca tttgaaatcc ttgaccgata tctgaaataa
    31081 gttcttctgt aatctcaaat ccttcatgct tataaacctc gattgcttga tgattcccct
    31141 gattcactcg caaaaagatc tctctttcct ttgtttgcgc agcaagttct tcataccagc
    31201 gaaaaactga agaagtcaat ccttgaccac gtactttatt tagcaaatat aatttactta
    31261 aataaatttt tccatttatc tcttcataag cagtgtaacc gacagtcttt tcttcgcttt
    31321 ttaataaaaa gtaggacaca ccttctgtca gttctttttg aatctgtttt tttgactgat
    31381 aagttttcag catgtaatcc acttgttttt gaccaatgat cggcacaaaa acttccggcc
    31441 atacttcttg tatgactgcc atcagctcat ccagttcatt ttctgttact tttatttttt
    31501 ccatacaccc acgtcctttt ttacattata aatgaccttg cgcttgatgg aataaaaaga
    31561 gcatgatcat gctatacaca cccaccgacc acggtttcaa gaaaatcaca atctgcataa
    31621 attccctaaa agataatttc gtcaaaccag ctactagaca gatcaagtcg tctggcgcaa
    31681 agggaagcag caaggtaaca actagtactt ttttaatatc tttcgttttt cttgtcagca
    31741 attcatcaaa tttctgatac ttttttggcg ataaaataag ctggacaaag gctacgccat
    31801 agtaacggac aagcaaaaat ccgattgctt ccccaatcac taggccaata caactatata
    31861 aaagcccaaa tccattgcca aacatcagca tgcccgctac tgaggagatc cctcccggca
    31921 aaataggaat gatcacttgg atcacttgca acagaacaaa tatcatcact gcatacttgc
    31981 caaattgctg gataaacgct tgcaaagacg tgctcgatcg aaaaatccca tgatgataag
    32041 cataaagaat caacacaaag aaaaaaaaca gaccaataat gggcatgatc tgaagacatt
    32101 tttgtaaaac tgatcctttt ttagacatct atattcccca catctttttt catttattaa
    32161 aactgcctcc t
//
LOCUS       DACCXM010000017        31297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCXM010000017 DACCXM010000000
VERSION     DACCXM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.018"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            211..1080
                     /locus_tag="IVY61_000689"
     CDS             211..1080
                     /locus_tag="IVY61_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP7969827.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1289..2314
                     /locus_tag="IVY61_000690"
     CDS             1289..2314
                     /locus_tag="IVY61_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7969828.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2353..3303
                     /gene="manA"
                     /locus_tag="IVY61_000691"
     CDS             2353..3303
                     /gene="manA"
                     /locus_tag="IVY61_000691"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP7969829.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3397..5100
                     /locus_tag="IVY61_000692"
     CDS             3397..5100
                     /locus_tag="IVY61_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7969830.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5102..7135
                     /locus_tag="IVY61_000693"
     CDS             5102..7135
                     /locus_tag="IVY61_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7969831.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7146..7586
                     /locus_tag="IVY61_000694"
     CDS             7146..7586
                     /locus_tag="IVY61_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7969832.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7786..8913)
                     /locus_tag="IVY61_000695"
     CDS             complement(7786..8913)
                     /locus_tag="IVY61_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7969833.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8916..9593)
                     /locus_tag="IVY61_000696"
     CDS             complement(8916..9593)
                     /locus_tag="IVY61_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969834.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9857..11623
                     /locus_tag="IVY61_000697"
     CDS             9857..11623
                     /locus_tag="IVY61_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP7969835.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11638..12075
                     /locus_tag="IVY61_000698"
     CDS             11638..12075
                     /locus_tag="IVY61_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7969836.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12088..13239
                     /locus_tag="IVY61_000699"
     CDS             12088..13239
                     /locus_tag="IVY61_000699"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP7969837.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13301..13999)
                     /locus_tag="IVY61_000700"
     CDS             complement(13301..13999)
                     /locus_tag="IVY61_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP7969838.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13992..15344)
                     /locus_tag="IVY61_000701"
     CDS             complement(13992..15344)
                     /locus_tag="IVY61_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP7969839.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15567..16967)
                     /locus_tag="IVY61_000702"
     CDS             complement(15567..16967)
                     /locus_tag="IVY61_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP7969840.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17128..17865
                     /locus_tag="IVY61_000703"
     CDS             17128..17865
                     /locus_tag="IVY61_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP7969841.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17990..18469
                     /locus_tag="IVY61_000704"
     CDS             17990..18469
                     /locus_tag="IVY61_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP7969842.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18466..19233
                     /locus_tag="IVY61_000705"
     CDS             18466..19233
                     /locus_tag="IVY61_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969843.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19463..20032
                     /gene="lepB"
                     /locus_tag="IVY61_000706"
     CDS             19463..20032
                     /gene="lepB"
                     /locus_tag="IVY61_000706"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7969844.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20136..20738)
                     /locus_tag="IVY61_000707"
     CDS             complement(20136..20738)
                     /locus_tag="IVY61_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7969845.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20844..21313
                     /locus_tag="IVY61_000708"
                     /pseudo
     CDS             20844..21313
                     /locus_tag="IVY61_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21419..22003
                     /locus_tag="IVY61_000709"
     CDS             21419..22003
                     /locus_tag="IVY61_000709"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7969846.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22006..23079
                     /gene="prfA"
                     /locus_tag="IVY61_000710"
     CDS             22006..23079
                     /gene="prfA"
                     /locus_tag="IVY61_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP7969847.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23072..23911
                     /gene="prmC"
                     /locus_tag="IVY61_000711"
     CDS             23072..23911
                     /gene="prmC"
                     /locus_tag="IVY61_000711"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP7969848.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23904..24083
                     /locus_tag="IVY61_000712"
     CDS             23904..24083
                     /locus_tag="IVY61_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969849.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24105..25130
                     /locus_tag="IVY61_000713"
     CDS             24105..25130
                     /locus_tag="IVY61_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP7969850.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25321..26565
                     /locus_tag="IVY61_000714"
     CDS             25321..26565
                     /locus_tag="IVY61_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP7969851.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26718..27665
                     /locus_tag="IVY61_000715"
     CDS             26718..27665
                     /locus_tag="IVY61_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7969852.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27799..28428
                     /gene="upp"
                     /locus_tag="IVY61_000716"
     CDS             27799..28428
                     /gene="upp"
                     /locus_tag="IVY61_000716"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP7969853.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28567..28809)
                     /locus_tag="IVY61_000717"
     CDS             complement(28567..28809)
                     /locus_tag="IVY61_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969854.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28930..29002
                     /locus_tag="IVY61_000718"
     tRNA            28930..29002
                     /locus_tag="IVY61_000718"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28963..28965,aa:Thr,seq:cgt)
     gene            complement(29121..29759)
                     /locus_tag="IVY61_000719"
     CDS             complement(29121..29759)
                     /locus_tag="IVY61_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7969855.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29882..30514)
                     /locus_tag="IVY61_000720"
     CDS             complement(29882..30514)
                     /locus_tag="IVY61_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7969856.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30865..31164
                     /locus_tag="IVY61_000721"
     CDS             30865..31164
                     /locus_tag="IVY61_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7969857.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10672 a         5460 c         6303 g         8862 t
ORIGIN      
        1 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
       61 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      121 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      181 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      241 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      301 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      361 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      421 gtgatcaaaa agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat
      481 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      541 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      601 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      661 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      721 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      781 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      841 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      901 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
      961 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1021 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1081 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1141 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1201 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1261 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1321 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1381 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1441 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1501 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1561 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1621 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1681 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1741 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1801 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1861 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1921 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     1981 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2041 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2101 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2161 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2221 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2281 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2341 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2401 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2461 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2521 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2581 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2641 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2701 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2761 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2821 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2881 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     2941 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3001 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3061 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3121 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3181 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3241 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
     3301 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3361 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
     3421 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg
     3481 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3541 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3601 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3661 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3721 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
     3781 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3841 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3901 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     3961 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4021 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4081 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4141 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4201 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4261 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4321 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4381 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4441 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4501 tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4561 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4621 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4681 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4741 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4801 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4861 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4921 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     4981 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5041 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5101 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5161 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5221 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5281 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5341 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5401 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5461 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5521 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5581 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5641 atcactgaat tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt
     5701 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5761 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
     5821 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5881 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     5941 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6001 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6061 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6121 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6181 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6241 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6301 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6361 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6421 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6481 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6541 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
     6601 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6661 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6721 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6781 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6841 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6901 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     6961 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7021 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7081 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7141 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7201 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7261 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7321 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7381 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7441 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7501 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
     7561 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7621 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7681 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7741 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7801 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7861 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7921 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     7981 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8041 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8101 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
     8161 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8221 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8281 ataaatggta aaacggatgc tatgtttctt taccatacta tccagtaata gattgactag
     8341 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8401 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8461 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8521 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8581 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
     8641 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
     8701 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
     8761 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
     8821 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
     8881 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
     8941 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
     9001 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
     9061 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
     9121 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
     9181 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
     9241 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
     9301 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
     9361 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
     9421 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
     9481 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
     9541 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
     9601 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
     9661 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
     9721 tttctgatac cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc
     9781 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
     9841 gagagggaga gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag
     9901 ggtacaaaaa ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat
     9961 tgcctggggt gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt
    10021 cgctaccatg gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg
    10081 cggaaaagta attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt
    10141 gattgtcgga acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg
    10201 atatgccatc aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat
    10261 gctagtcaat aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc
    10321 tgcgattggt ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat
    10381 tttatctgcc catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt
    10441 tttgaataat gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga
    10501 ggctggaaaa tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt
    10561 acttgctttt actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat
    10621 cattcacttt ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt
    10681 attattctta tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga
    10741 tgctggacta agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc
    10801 gttcaattct taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg
    10861 tatttctgca gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc
    10921 tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg
    10981 agctacattc tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag
    11041 tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt
    11101 gaccaatcaa gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt
    11161 cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa
    11221 ctttttgaat tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac
    11281 atcagtttct gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca
    11341 aatcaaagag attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac
    11401 cgtgctcaaa gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat
    11461 ccaagattta aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa
    11521 agtacagatg gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca
    11581 aaaatacgaa tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg
    11641 caaagtttgc cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg
    11701 atccgctatt gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat
    11761 gccatggtag atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct
    11821 catggaacag atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta
    11881 ccagatggtg tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt
    11941 attgcaggag tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt
    12001 gatatggaca atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat
    12061 ctagcaattg cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc
    12121 ggtcgaggat ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat
    12181 gtgaatcaca cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca
    12241 gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat
    12301 ccagaaaaag taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg
    12361 aatatcttac cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa
    12421 ggaagcacaa catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt
    12481 ctagaaaaag aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc
    12541 tttccaaatg cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc
    12601 gtccaagtgg aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa
    12661 atccaattaa aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta
    12721 tttagtgtca atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag
    12781 accattgatg aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag
    12841 gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat
    12901 attgaaaaaa tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc
    12961 gctcggacac cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa
    13021 ctacaagaac ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat
    13081 tactatgatc ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac
    13141 ttggaacaat tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc
    13201 aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga
    13261 atgtgacgaa atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa
    13321 gatagggcag atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag
    13381 ctgccaggtt ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc
    13441 ggtaaatgac accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc
    13501 ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat
    13561 aagtttcatt gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct
    13621 gacttaacgt gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca
    13681 tgtagtagtg acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt
    13741 cgatatagtt gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct
    13801 caaagtcttg accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat
    13861 gtatactaac gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca
    13921 tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat
    13981 tgagttcata attagccacg gttcattcct cctttgataa atccttgagc agctagttct
    14041 ttacgaagct gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat
    14101 tctttgattt tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga
    14161 atcccggcaa cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca
    14221 gctgggatat ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg
    14281 attccgtctg cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga
    14341 ccaatcatat cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat
    14401 tttttcccat tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa
    14461 ccttgtttga ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa
    14521 gcattgtaga cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg
    14581 aaatctgctg acacattgtc catccgtacc atatcagtta atcgataatc caattcagga
    14641 cgtttgaagt cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta
    14701 taatgcaaga tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt
    14761 tgttcacgat cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata
    14821 gaattgaaga ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca
    14881 ccgtctacaa tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg
    14941 aaaatatttg taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt
    15001 gcttcatcta tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag
    15061 aaagtcgata caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg
    15121 aattgctggc gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg
    15181 gtgacaacga cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc
    15241 agcgctagtt tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc
    15301 gacgtttttc caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa
    15361 acgatttgct gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt
    15421 gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc
    15481 atgatttaac aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat
    15541 tattccctct tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca
    15601 tagcctagat ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc
    15661 cgcccaataa acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta
    15721 atccctaaag ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc
    15781 agcgtttgtt cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa
    15841 agcataaaga caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata
    15901 ttgatgttat cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta
    15961 gttgttcgaa tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa
    16021 aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta
    16081 cgagtcgtaa tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg
    16141 aataaatggt ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca
    16201 aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa
    16261 cgtttgtata atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca
    16321 aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa
    16381 tccccagtga cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa
    16441 aaaacggcat cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat
    16501 gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg
    16561 ataccagaca aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg
    16621 atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca
    16681 acttgaaata atatttccag cagtataata ccgttatcat tgaacacaga attgatatca
    16741 aaagtcgata gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa
    16801 gaaacatggc tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc
    16861 gtcatcaata tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac
    16921 ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc
    16981 tgacaatagt cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca
    17041 aacagataac aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta
    17101 gaaaaaagaa ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg
    17161 aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa
    17221 gaagaagtaa agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca
    17281 ttggaattca tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat
    17341 cctaaaagtg agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat
    17401 gagaaaactg accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc
    17461 aaaatggttt taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca
    17521 tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac
    17581 tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga
    17641 atggatatcc aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa
    17701 ggctatgaac gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt
    17761 ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca
    17821 aaagcttact taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa
    17881 aaacgtaaga aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc
    17941 gtagtaaagt aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt
    18001 agcacctatt ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac
    18061 aggtattgtg aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga
    18121 aacaacacca actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga
    18181 aaccactacg acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata
    18241 tacaactgac agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc
    18301 aaccaatcca agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac
    18361 gactgtagat gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga
    18421 ttcaggaaat acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta
    18481 gaccttatca acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt
    18541 aaccgtggat atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat
    18601 ggactttggc aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg
    18661 cttgtttatt ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc
    18721 gatgtcactc agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa
    18781 gaatcgatag ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct
    18841 tatgacgaag attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt
    18901 gagatcaaaa caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg
    18961 tatccttatt actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga
    19021 agctactcgg acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg
    19081 gaacctgtcg gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat
    19141 gagccttatc gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt
    19201 tatagtcgta gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt
    19261 tgcgataaaa acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt
    19321 ttttatataa cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa
    19381 cattgtagaa taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga
    19441 aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct
    19501 tgtattagcg gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg
    19561 gcattcgatg gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa
    19621 ctatgaacga atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt
    19681 taaacggatc atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat
    19741 taatggcaaa aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga
    19801 taagttacaa ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc
    19861 cacctttacg agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga
    19921 taaccgcttg atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca
    19981 aggaaaagtt gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa
    20041 taaaaagaag ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat
    20101 ttcttggagt tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag
    20161 aaaagcgaag cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta
    20221 gcagagacaa agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta
    20281 tggactttcg gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg
    20341 ccaggagcta aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa
    20401 agtacaagtg atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca
    20461 gcgtggatcg ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc
    20521 agcatagttt ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa
    20581 ctaacaagcc cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt
    20641 agataagtag ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca
    20701 taatggttag cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac
    20761 tttgaaattt taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca
    20821 aaaagggaga atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag
    20881 gaaagtgtgt ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa
    20941 cggaaaaata tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag
    21001 aatatttagg ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta
    21061 ttgtatggga ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga
    21121 agcagaaggt tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg
    21181 gcactactct atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt
    21241 ggaagttttt ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt
    21301 ggaacaaaag tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta
    21361 aaaaatgctg aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat
    21421 ggcacaacta ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa
    21481 agtcgcacat aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga
    21541 tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc
    21601 gatatttgaa gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg
    21661 tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat
    21721 tgtggacgaa ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga
    21781 gttatttgaa ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac
    21841 aatctgttgg ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc
    21901 tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg
    21961 aaaacattac tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta
    22021 caagcaattg aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt
    22081 tctgatacaa agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc
    22141 gaagtttatc gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt
    22201 ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa
    22261 aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat
    22321 gacgataaaa atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta
    22381 ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca
    22441 gaagtgatgg aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc
    22501 accggagaca acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt
    22561 gttccttcaa ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg
    22621 ccagaagctg aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat
    22681 catgcttcag gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact
    22741 cacttaccaa cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt
    22801 gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa
    22861 agcgagtatg atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc
    22921 cggacgtata atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa
    22981 aaattagatc agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat
    23041 gatcaaacat caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct
    23101 gtcacgggct tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt
    23161 atttttggaa cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat
    23221 ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc
    23281 acctcagtat ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc
    23341 aacactgatc cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc
    23401 tggtgtaccg ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt
    23461 gaaagcagca agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga
    23521 agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac
    23581 attaggacca gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag
    23641 tcggaatgaa tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct
    23701 ttttgctgag aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt
    23761 actaaaatca aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa
    23821 aaaaatattt cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg
    23881 caatgaacga atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt
    23941 tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac
    24001 aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac
    24061 tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat
    24121 atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt
    24181 ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga
    24241 aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt
    24301 ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga
    24361 acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag
    24421 ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag
    24481 aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac
    24541 caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta
    24601 ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa
    24661 cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca
    24721 tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc
    24781 ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg
    24841 attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa
    24901 gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca
    24961 actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa
    25021 cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt
    25081 acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt
    25141 attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga
    25201 aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga
    25261 tgggaaaaat aaaattgtag gaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt
    25321 gtggtagatt acaaaacgtt tgaccctgat ctttgggctg cgatcgccaa agaagaagaa
    25381 agacaagaac ataatttgga gctgattgcc tctgaaaact ttgtctccga agcagtgatg
    25441 gctgcacaag gaagtatctt gacaaataaa tacgcagaag gttatccagg gcatcgctat
    25501 tatggtgggt gtgaatttgt cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa
    25561 ttattcggag cgaaatttgc gaacgtccag cctcactccg gctctcaagc caacacggct
    25621 gcttatctcg ctttggtaga accaggggac acgatcttag gaatggatct ttccgcaggc
    25681 ggacatctaa cacatggatc accagtcaac ttcagtggca aaacctatca ctttgttgct
    25741 tacggtgttg atccgacaac agaagttatt gattataatg tggtacgtat tttagctaga
    25801 aaacatcaac caaaattgat cgtagcaggt gccagtgctt acggacgaac gatcgacttt
    25861 gcgaaattca gagaaatcgc agatgaagta ggcgcaaaac tgatggtaga tatggcacat
    25921 atcgcaggat tagtagcagc aggacttcat cctaatccag ttccttatgc agatatcaca
    25981 acgactacca ctcataaaac attgcgtggc cctcgaggcg gcatgatttt gacaaacgat
    26041 gaagcattag caaaaaaaat aaatagtgcg gtatttcctg gtattcaagg cggccctcta
    26101 gagcatgtga ttgcgggtaa agcagtagca ttcaaagaag cattagatcc tgcatttaaa
    26161 gaatatagtg aacaaatcat cgccaatgca aaagcaatgg tcaaagtttt caatcaagca
    26221 attggaacca gagtcatttc aggggcgaca gataatcatc tgatgttgat cgatgttcgc
    26281 gagttaggta taaatggaaa agaagcagag agcattttgg acagtgtgaa tatcactgta
    26341 aataaaaact cgattccttt tgaaacacta agtccattca aaacaagcgg tattcgaatc
    26401 ggcacgccag caatcactac tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa
    26461 ctagttgtca aagcattaca agcaaaagat gataacgctc aactagacga agtcaaaaca
    26521 ggtgtacgcg aattaactga aaaatttccg ttgcataaaa aataaaaatt taaattccat
    26581 tagtaaacag aaaagttcaa tgttgggctt ttctgtttat tttttgggaa aacggattct
    26641 ttgacaatat cggtgatgct ctctagtata tgaggtgtaa ttttagtctt cacactaaat
    26701 ctcagggagg aaaagtcatg actgatcgta tctcgttaga aaaggaagca gtagcattca
    26761 gtgatgcaaa tgcgcctcat ccgcgtatct atgaactagc accggaagaa ggaagagttt
    26821 tactagaaga agtacaagaa tcacctgtga acaaatattc cgtcgatatt gaagacttga
    26881 tggtggatac tggcgaatgg ggaaaactca atattcgtct cgtacgacca gaagggaata
    26941 tggataaatt accagttatt ttttatattc atggagcagg ctgggttttt ggcagtgctc
    27001 aaacccatga taaattgatc agagaacttg cggttcgtac gaattccgtc gttgtctttc
    27061 cagaatatag tcgatcacca gaagcaaaat atccaacagc aattgaacaa aattatgctg
    27121 tgttgcaaca gctgaataaa ttagccaagt caaaaaatct ggatctcgag aatctaatcg
    27181 ttgcaggaga ttcagtaggt ggtaacatgg ctacagtcat gacgatctta acaaaagaac
    27241 gaaagggcct cccaatatcc aagcaattgc tgtattatcc agtcacaaac gctgattttg
    27301 atacagattc ctatcatgaa ttcggagaaa actatttttt gacaagagaa ggaatgatgt
    27361 ggttttggga ccaatatacg acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt
    27421 tgagagcaaa tataaatgaa ctaaatgatc ttccttcagc aatgatttta acgggagaag
    27481 cggatgtatt aagagatgaa ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc
    27541 ctgttacaca agtacgcttc caaggaatca tccatgattt tgtcatggta aatgctatgg
    27601 atcagacaaa ggcaacgcgt gcagcaatgg cattatctac ttcttggatc aagaaaaaag
    27661 aataagcttt gcttgtccgc aggaaaactt cttttcaaaa acggattttc ttaggaaaaa
    27721 tggcgagttt gactagtcaa tcacttgatt ttgttttaca attaaaaaga aacgacagaa
    27781 caaaggagag cttaaaaaat gggcaaattt caagtgatcg atcatccatt gattcaacac
    27841 aaactaacta ttattcggga caaaaactgc ggaacaaagg tttttcgtga agtagtcgat
    27901 gaaatcgcta tgttgatggc atatgaagta tcacgggata tgccattgga agacatcgtg
    27961 atcgaaacac cgatcactga aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt
    28021 attccaatct tgcgcgcagg tattggaatg gttgacggta tcttggaatt gattccagct
    28081 gctaaagtgg gacatatcgg tctttatcga gatgaagaaa cattggaacc acatgaatat
    28141 ttcgtcaaac ttccagaaga cattgatgca cgtcagttgt ttgtagtaga tccaatgttg
    28201 gcaacaggcg gatcagcaat catggcaatc gatgcattga aagaacgtgg cgcgtcgaac
    28261 attaaatttg tttgtttggt agcagcacct gagggagtaa aagctttgca agaagcgcat
    28321 cctgatattg acatctatac agcttctcta gatgaaaaat tgaatgaaca tggttatatc
    28381 gttcctggac taggggatgc gggtgaccgt ttgtttggta caaaataagt aaatacgaaa
    28441 aagggtgtga cacaactttt gtcacatcct tttttggtca tttttaacgt ttgatgcaga
    28501 acagcttttt cacagctttc gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg
    28561 tcaaattcaa atctttttta gttctttgta gttaacgatt actacaccct tactttgcgt
    28621 catcaagttc gcattttctg cagtttcatc aatctcaaca acagcggaat ttttcaattg
    28681 ctttgaaatc gttccagttt gcgacttgcc gtgaattgtg aacattacgt gagtattgat
    28741 cttgaatttt tcaccaattt ccgatggtac gatagttgta tcaaattttc caaaagttgc
    28801 cattttcata aaacctgcct ttcacatcaa ttataccacg ttttatgaaa aatatcatga
    28861 aattaaacta attttccatc ggactcttta caatcagcca aatagagtat ataatttctt
    28921 ttggataagg ccagcttagc tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta
    28981 ggttcgatcc ctatagctgg cattaaaaga gaagacagat atatcaagga tttttaatcc
    29041 attgatacat ctgtcttttt gtttaaagga aataaagtat gctttcttag caatagagca
    29101 tgtattattt ctataaaaat ttaggagaat aaaacgacta gcaatatagc tgtgaaatca
    29161 aatatcacat gaactgccca acttgtaatg atcgagttgc ttttaatggc agcttggttg
    29221 aataacaatc ttgccacccc ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt
    29281 ccattatcat aagtactgta atggataaat ccaaagatga ttgctgacaa gaaactagcg
    29341 acaggaatgc tgaacttact agaaaaaata gcgagaaaat acactgagaa caattcttct
    29401 ccaagtatca tgataggcaa aataataaat aataacgggc tgaaagcctc atttattgga
    29461 ttgcctgtta aaccccattt caaaatttgt tgcaaaaata ccgagactgc ggcactgatt
    29521 actacattga taaaaaaata tttggctata tttttgacag gagcgacagg tttcgagaac
    29581 atttttttcg tatctttttt accaaaaaca agtgtaaatg ctataaatcc agtgatagtt
    29641 aaagaaaaaa tgctaataat ggatggcgca aatagcaacc ctaacaaacc gagtacaacg
    29701 acaatagtgc ccaatatccc ttttttaagt tctatttttt ttcctaaaat gttattcatg
    29761 ataataactc ctactattca gtttttgaag atagagtcag tatacatgaa gagttaaaat
    29821 aggcaatgtt tttttatgga tatttgtatt gctaaaaatt tatcttatat attcattttg
    29881 attatactac ttttatttgt aaaaattttc tagcaaaacc aagtgcttgc aactgattaa
    29941 gcgtacaatt ttttagatgt tccgagcgta cttcaatcat ttcaaaggag ttgctactta
    30001 aatcgatatt ttttaaatca gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa
    30061 acattatttt agtgagggaa cagtctacaa aatctgcgct tgataagaca gagtgctcaa
    30121 aggtactttc ttttatgttt gaaccagata aatttacata gtccatttta caattctcga
    30181 ataacgtgtt cccaatataa ctttccatca atttagtacc tgttaattta caattgacaa
    30241 atttcacgcg atgcaacatg ctttttgtca atatacagcc tgaaaaatca cagttttcga
    30301 atatacagtc ggtaatctcc attctttgaa aatcattgga agcaaaaaat gcatacttga
    30361 aaatagattt gtaaataatt ggatggcaga ttatttggtt ttcgatgacc tctttttgtg
    30421 aaaggatatt atacagttca aaatcttcgt tttcatagat atgaaaaaag gatcctttat
    30481 tcaagcttgc ttcattaact ttaggaaaaa tcatatttaa ttacctccat tttcataaag
    30541 tcatattaac aaaagctaaa ccaacttaca ttagcagaaa atgagatagg ttttcgcctt
    30601 tttcgataac gatagtgtcg ctatctcttg aaacgttctt agaattgccc tgagagacac
    30661 aaattaggcg taattctaat aaaaatctta gaataaaaat ttttactgat ttaatttaga
    30721 atgtttttaa aataaaaatc gttgccataa cagtgtattg aaaggtttta acaatccagt
    30781 aatgatttaa aaaaatctag atgtagcgta tactttgttt atagaatgta gtgggaagaa
    30841 atatattttt gggaggaaat cgtgatgtct aaaagtgaag aaatagaaaa catggtacta
    30901 aaattatatg atctcgtttt aaatccagag atcaaagaaa aggaacgtat acttttgata
    30961 gacgcaaaga caggcttaga aaaaggtcag tattatccta aagtcataaa taatttggaa
    31021 cgtagtttac gtccgttagc aatcagggga gaattgtcta aaccagtatc accgttttat
    31081 atggagatat caacaatagg aaaatttgaa aaagaattag gtcgaggaat ggcttcggca
    31141 ccgattactt ttgggcattt ataaaaacga gttattcatc tcaatgaatt aaaaagtata
    31201 gtcaatctca taatgcaggc taggttcatt tatttgaaca gagtctgtat ttttttattc
    31261 taaataaaga aacagatgtt tctttatatt gtatttt
//
LOCUS       DACCXM010000018        29097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCXM010000018 DACCXM010000000
VERSION     DACCXM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.019"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1031)
                     /locus_tag="IVY61_000722"
     CDS             complement(<1..1031)
                     /locus_tag="IVY61_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7969858.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IVY61_000723"
     CDS             complement(1113..1802)
                     /locus_tag="IVY61_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7969859.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IVY61_000724"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IVY61_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP7969860.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IVY61_000725"
     CDS             complement(2329..2832)
                     /locus_tag="IVY61_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP7969861.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IVY61_000726"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IVY61_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP7969862.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IVY61_000727"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IVY61_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP7969863.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IVY61_000728"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IVY61_000728"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP7969864.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IVY61_000729"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IVY61_000729"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP7969865.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IVY61_000730"
     CDS             complement(6444..7463)
                     /locus_tag="IVY61_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7969866.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IVY61_000731"
     CDS             complement(7636..8523)
                     /locus_tag="IVY61_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7969867.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IVY61_000732"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IVY61_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP7969868.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IVY61_000733"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IVY61_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP7969869.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IVY61_000734"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IVY61_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7969870.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IVY61_000735"
     CDS             9754..10398
                     /locus_tag="IVY61_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7969871.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IVY61_000736"
     CDS             complement(10454..10957)
                     /locus_tag="IVY61_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7969872.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IVY61_000737"
     CDS             11554..12519
                     /locus_tag="IVY61_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7969873.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IVY61_000738"
     CDS             complement(12618..13790)
                     /locus_tag="IVY61_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7969874.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IVY61_000739"
     CDS             complement(13777..14922)
                     /locus_tag="IVY61_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7969875.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IVY61_000740"
     CDS             complement(14936..15868)
                     /locus_tag="IVY61_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969876.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IVY61_000741"
     CDS             15985..17064
                     /locus_tag="IVY61_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7969877.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="IVY61_000742"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="IVY61_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17789..18016)
                     /locus_tag="IVY61_000743"
     CDS             complement(17789..18016)
                     /locus_tag="IVY61_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7969878.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="IVY61_000744"
     CDS             complement(18119..20707)
                     /locus_tag="IVY61_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP7969879.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="IVY61_000745"
     CDS             complement(20727..21386)
                     /locus_tag="IVY61_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7969880.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="IVY61_000746"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="IVY61_000746"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP7969881.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="IVY61_000747"
     CDS             complement(22159..23025)
                     /locus_tag="IVY61_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7969882.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="IVY61_000748"
     CDS             23134..23775
                     /locus_tag="IVY61_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP7969883.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="IVY61_000749"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="IVY61_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP7969884.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="IVY61_000750"
     CDS             complement(25216..26115)
                     /locus_tag="IVY61_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7969885.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="IVY61_000751"
     CDS             complement(26117..26914)
                     /locus_tag="IVY61_000751"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP7969886.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="IVY61_000752"
     CDS             complement(26892..27596)
                     /locus_tag="IVY61_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP7969887.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="IVY61_000753"
     CDS             27727..28302
                     /locus_tag="IVY61_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP7969888.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="IVY61_000754"
     CDS             28420..29097
                     /locus_tag="IVY61_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7969889.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8560 a         6167 c         5232 g         9138 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaa
//
LOCUS       DACCXM010000019        29096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCXM010000019 DACCXM010000000
VERSION     DACCXM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.020"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            278..1063
                     /locus_tag="IVY61_000755"
     CDS             278..1063
                     /locus_tag="IVY61_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7969890.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1076..1927
                     /locus_tag="IVY61_000756"
     CDS             1076..1927
                     /locus_tag="IVY61_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP7969891.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2051..2503)
                     /locus_tag="IVY61_000757"
     CDS             complement(2051..2503)
                     /locus_tag="IVY61_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7969892.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(2968..3336)
                     /locus_tag="IVY61_000758"
     CDS             complement(2968..3336)
                     /locus_tag="IVY61_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP7969893.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3486..3968
                     /locus_tag="IVY61_000759"
     CDS             3486..3968
                     /locus_tag="IVY61_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP7969894.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4005..5015
                     /locus_tag="IVY61_000760"
     CDS             4005..5015
                     /locus_tag="IVY61_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP7969895.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5130..5843)
                     /locus_tag="IVY61_000761"
     CDS             complement(5130..5843)
                     /locus_tag="IVY61_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969896.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(6033..6623)
                     /locus_tag="IVY61_000762"
     CDS             complement(6033..6623)
                     /locus_tag="IVY61_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP7969897.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7107..7334
                     /locus_tag="IVY61_000763"
     CDS             7107..7334
                     /locus_tag="IVY61_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP7969898.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            7350..8516
                     /locus_tag="IVY61_000764"
     CDS             7350..8516
                     /locus_tag="IVY61_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP7969899.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            8750..9793
                     /gene="hrcA"
                     /locus_tag="IVY61_000765"
     CDS             8750..9793
                     /gene="hrcA"
                     /locus_tag="IVY61_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP7969900.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            9887..10450
                     /gene="grpE"
                     /locus_tag="IVY61_000766"
     CDS             9887..10450
                     /gene="grpE"
                     /locus_tag="IVY61_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP7969901.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            10501..12330
                     /gene="dnaK"
                     /locus_tag="IVY61_000767"
     CDS             10501..12330
                     /gene="dnaK"
                     /locus_tag="IVY61_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP7969902.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            12481..13647
                     /gene="dnaJ"
                     /locus_tag="IVY61_000768"
     CDS             12481..13647
                     /gene="dnaJ"
                     /locus_tag="IVY61_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7969903.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(13729..14394)
                     /locus_tag="IVY61_000769"
     CDS             complement(13729..14394)
                     /locus_tag="IVY61_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP7969904.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            14370..14543
                     /locus_tag="IVY61_000770"
     CDS             14370..14543
                     /locus_tag="IVY61_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7969905.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(14588..15325)
                     /locus_tag="IVY61_000771"
     CDS             complement(14588..15325)
                     /locus_tag="IVY61_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7969906.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(15327..16829)
                     /locus_tag="IVY61_000772"
     CDS             complement(15327..16829)
                     /locus_tag="IVY61_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7969907.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            17088..19082
                     /gene="uvrB"
                     /locus_tag="IVY61_000773"
     CDS             17088..19082
                     /gene="uvrB"
                     /locus_tag="IVY61_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP7969908.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            19092..21908
                     /gene="uvrA"
                     /locus_tag="IVY61_000774"
     CDS             19092..21908
                     /gene="uvrA"
                     /locus_tag="IVY61_000774"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP7969909.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            22171..23436
                     /locus_tag="IVY61_000775"
     CDS             22171..23436
                     /locus_tag="IVY61_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP7969910.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            23440..24171
                     /locus_tag="IVY61_000776"
     CDS             23440..24171
                     /locus_tag="IVY61_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP7969911.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            24306..25190
                     /gene="rapZ"
                     /locus_tag="IVY61_000777"
     CDS             24306..25190
                     /gene="rapZ"
                     /locus_tag="IVY61_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP7969912.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            25187..26185
                     /locus_tag="IVY61_000778"
     CDS             25187..26185
                     /locus_tag="IVY61_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP7969913.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            26219..27154
                     /gene="whiA"
                     /locus_tag="IVY61_000779"
     CDS             26219..27154
                     /gene="whiA"
                     /locus_tag="IVY61_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP7969914.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            27697..28347
                     /locus_tag="IVY61_000780"
     CDS             27697..28347
                     /locus_tag="IVY61_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969915.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            28534..>29096
                     /locus_tag="IVY61_000781"
     CDS             28534..>29096
                     /locus_tag="IVY61_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP7969916.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         9565 a         4897 c         6176 g         8458 t
ORIGIN      
        1 aaggggctgt gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa
       61 gtagcttctt cggaaataag ccgaaatttc ttgaagctga ccacttctgt cacaacctct
      121 ttctttatag aaataaaagg acactagcaa ttcatgaaaa cgattgctag agcgtatcat
      181 ttgctttaga ctatatatct tggtgtacac tcactttaag gcggaaataa aagttatccg
      241 taataacagg tttaggtatg aaaaaggagt gggtactatg tacaaggaat tagaaaacaa
      301 agtcgcagta gtaactggtg gctcaaaggg gattggaaca gcgattgctc aacgattcgg
      361 gaaagaaaaa atgaatgtag ttgtcaacta taactcggat aaagaaggag cagaaaaagc
      421 agtcaaagca atcgaagagg caggcggaaa agcagtctct gtacaagcag atgtcagttc
      481 tgaagaagga atacagcgtt tacttgatac ggctttagaa aattttggga cactggacgt
      541 ttgggttaat aatgctggga tggaaaatca gcatccaacc catgaactta gccttgaaga
      601 ttgggaaaga gtgttaaaag tcaatttgac tggcgttttt atcggaacga aagcggcttt
      661 aaattacttt acagagaata ataaaaaagg aaatatcatc aatatgtctt ctgttcatga
      721 acagatccca tggccaacgt ttgctcatta cgctgcttca aaaggcgggg taaaattatt
      781 taccgaaacg gtagcaatgg agtatgcacc gaagggaatc agaatcaata gtataggccc
      841 tggtgctatc cgtacaccta ttaatgcggc aaaatttgat gatcctgaac aaaaagaact
      901 cttggaaagt atgatcccac taggacgcgt aggtgaacca aaagaaatag cggcgacagc
      961 tgcatggttg gcttcagacg aatccagtta tgttacaggt attacactat ttgcagatgg
     1021 tgggatgact ttatatcctt ctttccaagg tggaaaagga taagggtgaa taaaaatggg
     1081 tttattgatt gcattgatcc cggcagttgg ctgggggatc cagccactga ttttgaaaaa
     1141 gattggtggc agaccaacaa atgagatatt aggaactggt attggagcgg tgcttgtcgg
     1201 cttactcgtt caattattcg cttctccagc tggtatttca gtgactacat ttttgattag
     1261 tttactttct ggtgcatttt gggttattgg acaaacagga cagtatacat ctttcaactt
     1321 gattggtgtt tctaaaacga tgccaatttc gactgcatta cagttagtcg gaacctcgtt
     1381 gattggtgtc ttcgcttttg gagaatggtc tggaagtatg gggaaaatca ttggagcagc
     1441 agcaatcatt cttcttgtat ttggttctgc tttaacggca atcagtgatg atcctggaaa
     1501 aaaaggcggt atcacaaaag gaattagtct attagcatca acaagtattg gttattgggt
     1561 atacagtgca ttgcctaaac tagttgatgc aagcggactt tccattttct ttccgcaaat
     1621 gatcggtgtc tttttagggg cagtgattta tgtgattttc cgtaaaccaa ccgcattttc
     1681 tgaagacaag agttggaaag caacgatcgt tggagccatc tttagtgtgt cagcattggc
     1741 ttatattttt tcagcaaatg aaaatggggt tgctactgca tatatcatta cacagctaaa
     1801 cgtagttatc tctacattag gcggtatgta catcctgcat gaaaataaga caccaaaaga
     1861 aaaaaaatta acgattatcg gattattgct gatcgtagta ggaagcatgg tcacggtctt
     1921 tatttgagga taaagatggt aaaatttcat ttattaaaaa aatatttgtt tgaaccaaac
     1981 agtaagtact tataaaaaaa tagcgctaga atttccaaca tctctggaat ttctagcgct
     2041 ttttaattgt ttaagataca agggaaatat ctgtctggat caataatttt tcattgatcc
     2101 attgactaaa gtttacgaca taattgaagt taccaccgat tagtatctcc gcatgattgt
     2161 ctttataaag atcgtattgt aaaagacggt cttttttatt tgcgtaaaca tcgttatggt
     2221 actggactaa ttgttctcgg aattgattga ttgctgtaaa atcacgatat tgaaggttga
     2281 aacgcatcgt gattggtgca tttagcatga atcgctgcat ttcttttgct gcttcctctg
     2341 cttgatctcc gtataggaac tcaaaaagat tacgttcaga aaatttccat tgcccgccaa
     2401 cctttgttcc ttttagtttt ccttcatgaa gataatttct gatagaacgt gtagtaacac
     2461 ctaattgttc ggctaattca tttactgagt aaaaatcttt caatgttcac cctcctatgt
     2521 gaatggttct actgacttag ttgatatttg cttaatcacg aaatgtagaa agaacgattt
     2581 taattaaggt cattgtaaaa atcaagtcag aaaattctaa cttttatttc tctaaagaaa
     2641 ttcgttctct cctaaagtga tattatattt aaatatacct gaaaataatg ggcagtatta
     2701 tgaaagatca ctgatatttt ctcgtatttt ttccttattc aaaagttttg caggaaaatt
     2761 gctgaaaaaa taattttact aagtagaaaa ttcaaagcca ttcaatcagt gatttttttc
     2821 agcacaagaa agactcctta taaaaatagc agatactttg tcaattaagt gtttaaccag
     2881 ggatgataaa aaggatagga ttgaacaaaa cctgtataag gtgatgaata gtttcctcgc
     2941 cagtaaaagg tttattcgta taaagcttta tcgccaaaat ttatcatgac cttttttatc
     3001 ttcaatatta aatgaaatca attgttcaat caattcccag ttgattacac ttttccaagg
     3061 aactttgatg acgttatccg tatatacgta gcctgcttct tggatatttt tttcaagggc
     3121 acgaatcgct gctttttccg gtgcgataga aaaatgattt ttagcaaaac taaatgcaat
     3181 aataaaggta ccatggtcag taaacatagg ttggttccat tttacagttg tgtctagctg
     3241 gggatacgtt tctgttatct tagcaaaaag gtctttgatc ttttgttgat gttctttttc
     3301 tttgatttga ttgatgatcg gctgaaattt ttccattcta aaacgcctcc attaagtggt
     3361 ttgatttatt ttaccagaaa aacaatagtt atgtataaaa aacgaacaaa acacaagtaa
     3421 tgttgtaacc gctatattta tatgataaga tgtctcaaat aataaaatag aaaagagaca
     3481 taaaaatgat tgacttggca aagaaattct tacacgaaaa tatatatttt gagttgtatc
     3541 atcatcgagc catttataca aatgaagatg ctttggtaat aaagaaagaa caaggattta
     3601 caggtacaga gaccaaaagc ttattcttaa aagataaaaa agataataac tacgtatatc
     3661 tgacttttac tacgaaacaa acggatttca aagcattagg gaagatcgtc ggtaaaaggc
     3721 tttctattgt ttcatcagaa cgcatggaag aacaaacggg acaaaagcag ggcgctgttt
     3781 ctccgtttgg ttatgaaaaa cagattccga ttattattga tgaagagctc ttatcaaatg
     3841 aaaaacttgt ttttgcacca ggtagaccag atcaaacgat ggtcatccaa acggttgact
     3901 taaacaaaat catccagcta cttgagatgc cgatattttt gatgccggca aacgaaaaaa
     3961 acaattaacc aagtaaaata gtgcatactt ggaggtgaag caacatgata agttatccat
     4021 tattagaaag aaatgcaacg attggggtta ctgcaccatc ctcaggcgta tcagaggaac
     4081 accattcgtt actcgaatca gctttaaaga gaatggaaga aaaatctttc caagtagtta
     4141 ctggaaagac gctttggacg caagataagg aaaaatcagg tccagcaaga aaacgagcag
     4201 atgaattcaa tcaaatgatg caagatgaaa atatccaaat gatctttccg acttggggag
     4261 gagaattatt gattgagatt ctcgattttg tcgattttga tgctctacaa ccaaaatgga
     4321 tagtaggtta ttcagatatt agtacgttgc tacttgctgt cacattaaaa acagggatag
     4381 ccactgcaca tggaactaac cttattgatt taagaggaga gtacttggat gaaacgacag
     4441 caatgtggga aagtgtactg aaaacaagag aaggagaatc gattctacag caatcttctg
     4501 aaaagtatca aaaagtgtgg ccccatgagc attcatcatc ttctgttttc catctcactg
     4561 aaccaacaaa ttggcaatca gtcagcggag atgcagtaag gatgaaggga cgattattag
     4621 gtggttgtat tgatacgatc aggcatttaa tcggtactcc ttatggagat gtccaatctt
     4681 ttaaaagaaa gtataatgat ggaaagccga tcttgtggtt tctagaaaac tgtgagctat
     4741 cgtcagttga tttaagaaga tcacttgttc agatgaaact ggcaggctgg tttgatggca
     4801 gctcggggat catgttcggt agaagtgcgg cagaggattt gttagattat aaaatgaagg
     4861 atgtatacga agatctgtca aaggaattaa aactaccgat cttgtatgat attgattgtg
     4921 gtcatgtacc gccacaactg acattgatta atggtgccta tgcagaagta gcggtagaag
     4981 atagaaaagc gactgttttg caaacgttta tttaaaaaat tttttcctgc cttggagtct
     5041 atcaaagacc aataaatcta aatagaaaag aaagtgaaag aagctgacaa aatgctgtca
     5101 gcttcttttt gtataacata ttagaaagtc taattatcta taactgtcac atcgattaac
     5161 tgtttcacgt cattgggatt tcctgtagga atagctacta cagatgcatt atcaatgatt
     5221 tcttgagttg tttctctaat gaacgctgga taagatgttt gatctgcagg catttgccaa
     5281 tgtgaaggaa ctggataact agtgattgtc ccgtcatggt tacttgaata cactaaaact
     5341 ccctggtaac tgtatttccc agtgagcata accacatctt tcggatagac tgcactccct
     5401 gcatcataag gggcgatagg tgttcctgca ggataatgaa taacatgtaa ttcgaaatca
     5461 cccgatgtat ctctaaaagt attacctcta atagctagcc aaacacgtgc gtactcaatt
     5521 tgtagatcag aatatcctgc taacggatct attgactgag aagactgtga ggaggttgtg
     5581 tctattgttg aagaagaaat atttgtctca actgtactac tagatgtaga agaagtattg
     5641 ggctctgaga ctttactgct tgttgaagat gtattcgttt cagtagaagc tgatttctga
     5701 gtacttgatt tttcagctga actggtttgt ctgattgaac ttttgtctgt ggtcgcagat
     5761 ggatcactat tttctttact aggagacgta catcctacaa ggaataaaga gaccaacaat
     5821 aaaataaaac ctaacttttt catataaaat acctcattat ttttttaata ccagtatagc
     5881 ataatcctga atttttgaat ccgtaaaacc ttgtctatga atataaatag agtgtatctt
     5941 ctttgataaa tgtcagaaaa agtgtgacaa ataatcatca gtaaaattac ttgtcacact
     6001 attgataagg agtatttaat tgaagagaat gattaggaga aaggatctcc gtacttttta
     6061 tagtaatcat agaatccaag tttgtataga cgaatcgttt tttctcttat ttcttctgtt
     6121 tcaaaatgga ctgcttgaag aatcaagtct gtgaattcta taggagctgt tccattcgca
     6181 gtaatcaaat tacgatcttt cacagcttgt tgttctataa aatcttcttt attctgataa
     6241 ttttccgatt ctaaccaagg aaattgcgca tttccggtgt gtttgtaacc ttgcaataat
     6301 ccgctctgag ctaaataatc aactgcccca caaatagcag ctactggtac gtctgttttt
     6361 aacaatttat cgattatttg atataaatga tgatttttta tgtcccatga attgccaccg
     6421 atcataataa aaaggtcaac tttttcaggt atttgttcga ttaagtaatc gatttttgtt
     6481 tttaatcctc caatggaagt gacctctgat tggatagaag cagttttgtt ttgccatttc
     6541 tcattttggt ttagtgtgct agtaatataa gcaccttccc aatcggcata ttcctctaat
     6601 agaaacgtaa tcgcagtttt cattttcaag ctctccttta ctatctataa caaggacaac
     6661 agttttaacc tacagacatt cattaaaatt gggtagcgac agcgaaaaca acaattctta
     6721 agttttagac agagtataca gatagtcaac ttaattaaat acgaactaaa taaaaatgac
     6781 aggtaatcat ttttgtttac cagcattata acattttgca tacatggatg acaaaagaac
     6841 caattcccac ttgcttgagt acaatattaa aagaaactgg aaggcaaaaa gtaattatag
     6901 aaacgaaaaa taaatcatat cgggcactca gacatgaaaa caaccctaga gatatataac
     6961 taggtaacca gtttaatgaa agaaaaaatc atccaagaag tcgattcttg gattttttag
     7021 tggatttgtt cgccaaatac gtttagtatg cgtatatgag aggttgtaag caaagcgttg
     7081 tggtctgagc acttaaggag gaatctgtga aaatagcttt tcatattttt gcagattttg
     7141 acttaatgcc gaaggtcagc tttgcgatta ccgtttatcg agaggctgta aaagcaccgt
     7201 gtagctccga tgacaataag caagcatttt tagagctagg tttccttgat tctaaatatg
     7261 cggcttattt caatgagttg gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg
     7321 acttttcgac gtaaggagaa agaatttcaa tgatagaaac aataaatccc gcagaaaaca
     7381 aggtttctgc gtacattcat atcccatttt gcgagcatat ctgctattac tgtgatttta
     7441 ataaagtttt tttagaaggg cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa
     7501 tgaaaataat gctagaaagt catccgacag cgaatttaga aacactttac gtagggggcg
     7561 ggacaccaac ttctttatct gctaaacaac ttgatcgact gttatcaggt gctagagaga
     7621 tcctgccttt tcaaaatggt aatgagttta ctgtagaagc gaatccaggt gatttgaccc
     7681 gtgaaaaact tcaagtaatg aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga
     7741 catttgataa tcgcttattg aaaaaaattg gacgtaaaca caccgcagaa gatgtgtatc
     7801 aaactatgca atttttagaa gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg
     7861 cattaccggg acaaacacta gaaggatatc tggatacttt ggaaaaagct ttggctttgg
     7921 atctgcctca ctattctttg tactcattga tcctcgaaaa caaaacaatg ttcatgaact
     7981 gggtacgaca aggaagactc gaactgcctg atcaggaaac ggaaaccaga atgtttgaag
     8041 agacgattga agcaatggaa aagcatggac gtcaccaata tgaaataagt aactttgggc
     8101 tctccggaca tgaatctcaa cataacctca tgtactggaa caatgcccac tattttgggt
     8161 ttggagctgg tgctagcggt tatcttggag atattcgtta ccgcaacaaa ggaccaatcc
     8221 aacactattt acgcccatta cgagcaaatg aacgcccggt attggaagaa gaaaaattga
     8281 cacgaaaaaa tcaaatagaa gaagaaatgt tcttaggact aagaaaaaaa acagggatta
     8341 ctaaaaaaca tttctatgat cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc
     8401 taagggaatt agagcaagaa gggttacttg ttaatgaaga agaccgtgtt tatttgacac
     8461 caagagggac ctttttgggg aacaatgtgt ttgagcgctt tttgtttgaa aaatagttta
     8521 aagaatattt gttttttatc tgaatttaaa taaaaaaagt tcaaaaatac aataaaggac
     8581 tattgaaacg ttgatataac tggatttaca tcaattattt agaattagca ctcttgtgtt
     8641 tagagtgcta aaaaatggag tctttctctt gacaggagaa gggcttcttg ctatatttat
     8701 aagtggatta gcacttagaa tatatgagtg ctaatgagag gtgaggaaga tgttaacaaa
     8761 gcgacaaaac gacattttac gtctgatcat ccaacactat acgagtagtg gcgtgcccgt
     8821 tggttccaaa acattgatgt ctgagggagt caaggccagc tcagcaacga ttcgcaatga
     8881 tatgaaagca ttagaagatg aaggattgct gttgaagact cactcttctt ctgggcggat
     8941 tccatctgct ttaggctatc gttattacgt ggatcatttg ttaaaacctg ctcgcgtagc
     9001 agaagatgat ttgctgctga ttcgtcagtc tctaggcaga gaatttcatg aaatcaatga
     9061 gattattaag caatctgctg agatattatc tgagctgaca agctatacga ctttttcatt
     9121 gggacctgaa ataaaagacc gccgactgac tggctttcgt atcgtaccct tgaataatcg
     9181 acagattatt gctatcgtcg taacagatat ggggaatgta gaaagccaag tgttcactct
     9241 tcccgaatat atgggaagtc aggaactaga aaaaatggta cagattgtaa acgaccgtct
     9301 agtcggagat cctctgatga cggtgtacca taaacttcgt acggagattc cgatgattct
     9361 tcataaatat ttccaaacga cagaagggat gtctcagtta tttgacaccg ttcttggtca
     9421 tgtttttgaa gataaggttt atgtcagtgg acaaatgaat ctcttggatt atgagccccg
     9481 tcaagatctt gatcagttca aatcgatgtt ttcttttatg aaagactcag acggacttac
     9541 acaaatgatc actccgatgg atagcaatat tcatatcaag attggtgctg aattaggcaa
     9601 tgatttattg cagaatatga gtatgattca agcaagttac gagatagatg gtcatggaag
     9661 aggtacaata gccttgttag ggcctactag tatgccttat tctcggatgt tcggtctagt
     9721 cgatgtttat cgaagagaac tagcagctaa acttgctgat tactaccgtt cgcttgactt
     9781 gtcaaactcg taatgtgaat aacgaagcta tttgcagctg atcgttctct ctttcttagt
     9841 aagagcaatc aaacagctga ctgaataatg aaaggaagac tgtatcgtga aagaaaatca
     9901 agaagaacta gataaagaaa tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc
     9961 tgcagaagat tcgggtattt cagaagctga agcggaagaa ttcgaaacag cgaaattaaa
    10021 agctgagtta gaagaaatgg aagataaata cttgcgggct cgtgctgaaa tcgcaaatat
    10081 ggcaaatcga ggaaaaaatg agcgtgagca attacaaaaa taccgttcac aagatttagc
    10141 caaaaaatta cttccatcaa ttgacaacct ggaacgtgca ttagccacag aagtgtcaga
    10201 tgatcaaggg gcaggtttga aaaaaggcgt ggaaatggtc ttggaaagtt tgcgtaacgc
    10261 actggaagaa gaaggaattg aaaaaatccc tgctaaagga gaagcctttg atcctaatct
    10321 tcatcaagca gtacaaactg ttccagcgac tgaagataca ccagccgata cgattgtaga
    10381 agtactacaa gaaggctaca aattacatga tcgcgtgtta agaccaacca tggtcattgt
    10441 tgcacaataa ttgtgcggat ataaaactta cactttcaat taaagaagga gacgttaatt
    10501 atgagtaaaa ttatcggtat tgacttaggt acgactaact ctgcagtagc agtattggaa
    10561 ggaaacgaag caaaaatcat cgctaaccca gaaggaaacc gtacaacacc ttctgttgta
    10621 tcattcaaaa atggagaaat ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac
    10681 ccaaatacga tttcatcaat caaacgccat atcggtgaag caggttacaa agtagatgta
    10741 gaaggtaaat catatacacc acaagaaatc tcagcaatga tccttcaata tatcaaagga
    10801 ttcgcagaag actatttagg tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac
    10861 tttaatgatg cacaacgtca agcaacaaaa gatgctggta agatcgctgg tctagaagta
    10921 gaacgtatcg tcaatgaacc aactgcagct gctcttgcct atggtttaga taaaacagat
    10981 cgcgacgaaa aaatcttagt attcgacctt ggtggcggta cttttgacgt atctatcctt
    11041 gaactaggag acggtgtctt tgatgtattg tcaacagctg gcgacaacca tctaggtggg
    11101 gacgactttg ataacaaaat catcgactac atggtagcag aattcaaaaa agaaaacagc
    11161 attgacttgt ctcaagacaa aatggcttta caacgcttga aagacgctgc tgaaaaagcg
    11221 aaaaaagatt tatctggcgt atcaagcaca caaatcagct tgccatttat cacagcagga
    11281 gaatctggac cattgcattt ggaaatgaca ttgacacgtg caaaatttga tgaattaaca
    11341 gctgacttag tagaacgtac aaaagtacca gtacgtcaag cgctaaaaga tgcaggttta
    11401 tcacaatcag aaatcgacga agttatttta gtcggtggat ctactcgtat ccctgcagtt
    11461 gtagaagcag tacgtaaaga aacaggaaaa gaaccaaaca aatcagttaa ccctgatgaa
    11521 gtagttgcta tgggtgctgc tatccaaggt ggcgtgatca ctggtgatgt gaaagatgtt
    11581 gttttacttg acgttacacc attgtcatta ggtatcgaaa caatgggtgg agtgttcact
    11641 aaattgattg accgcaatac aacgattcca acaagtaaat cacaagtatt ctcaactgcg
    11701 gcagataatc aaccagcagt agacatccat gtattacaag gtgaacgccc aatggctgct
    11761 gacaacaaaa ctctaggaag attccaattg acagatattc cagcagctcc tcgtggtatt
    11821 ccacaaatcg aagtaacatt cgatattgat aaaaacggga ttgtaaatgt atctgcgaaa
    11881 gatctaggta cacaaaaaga acaaaaaatc acgatcaaat cttcttcagg tctaacagac
    11941 gaagaaatcg aacgtatggt aaaagatgca gaagcaaatg cagaagcaga taaagctcgt
    12001 aaagaggaag ttgacctacg taatgatgta gatgcattgc tattctctgt ggacaaaaca
    12061 ttgaaagaat tagaaggcaa agtggacgaa gaagaagtga aaaaagcaga aacagctcgc
    12121 gatgaattga aagccgctgt tgaagcaaac aacatcgaag aaatgaaaac taaacgcgat
    12181 gcattgaacg aaatcgttca aaacttgact gttaaactat acgaacaagc ggcacaacaa
    12241 caagctcaag aaaacccaga agcagctcaa ggcggagcag atgatgtcgt tgatgctgat
    12301 tttgaagaag tcgatggcga cgataaataa tcagttcact gaatttcgag agaaggccaa
    12361 agttgctact ttggcttttt cttttgaaaa atagtttgaa gtatcggttt ttgtttcaaa
    12421 caaaatatga tatgataaca agcgacgtga aaatgatgag aaccaatagg aggaaagcct
    12481 atggcaacaa aacgtgatta ttatgaagtc ttagggctct caaaaggagc atcagaagat
    12541 gaaataaaga aagcttatcg taagctttcg aaaaaatacc atcctgacat caataaagaa
    12601 cctgatgcgg aagaaaaatt caaagaagta tcagaagcat atgagatttt aagtgacccg
    12661 caaaaacgcg ctgcatatga ccaatacggg catgcaggca cagatccaaa ttacggtgct
    12721 ggtggagctg gtggcggatt tggtggattc ggcggcttct ctggtggcgg cttcggtggt
    12781 tttgaagata tcttcgactc tttctttgga ggaggcggtc ggtcagtcga tccaaatgcg
    12841 cctcgtcaag gtgctgattt gcaatatacg gtcgacttgt cttttgaaga agcgattttt
    12901 ggtgtagaaa aagaaatcaa gtataatcgg gaagaaatct gtcatacttg tggtgggaac
    12961 ggtgcaaaac caggcacaca accaactact tgtcataaat gtcatggtgc aggtacgatt
    13021 aatgtagaac gccaaacacc acttggacga gtgatgagcc gtcaaacttg tgatgtttgt
    13081 catgggacag ggaaagaaat caaagaacct tgtccaacct gtcatggctc aggacatgag
    13141 aagaaagcac attcagttaa agtgaatgta ccagctggtg tagaagaagg acaacaaatg
    13201 cgtttagctg gtcaaggaga agctggagaa aacggcggac catttggtga tttgtatgtg
    13261 gtcttccgtg tagaagaaag tgatatcttt gatcgtgaag gttctgaaat ttactatgaa
    13321 ttgcctttaa gttttgtaca agcagctttg ggggatgaag tccaagtacc aactgtccat
    13381 ggagatgtga aattgaagat ccctgctgga acacagacag gtacgaactt ccgtttacgt
    13441 ggaaaaggcg ctccgaagtt acgtggtggt gcaacaggcg accaacatgt caaagtaaaa
    13501 ctgatcacac cgaaaaatct aaatgaacaa caaagagaag cattacgtgc attcgctgaa
    13561 gctggcggca tcaaagttga agaacagcaa gaagacggtt tcttcgataa gtttaaagat
    13621 gcttttggcg ggaaaaagcg gaagtagttt ttctagtaaa aacgctaagt cattaataat
    13681 taaaaaccag acgaacaatc atatgttcgc ctggtttttt tgtagttgtc atgtttgtac
    13741 caacaaaaaa gccagcttta gaatatgata atcgtcatag tttctagggg tataaccagt
    13801 gagagaatgg aacttgttca atcgatattg caaggtgttt ttatgaataa ataattcttc
    13861 tgagcaattt ttgatgcttc cgttattttt actgaagcat gccaaagtct tcttgaacaa
    13921 atgtacttct tcttctgtca ggcttttcaa aatacgtttt acatataatg ttcgttgatc
    13981 tgacgggatt gctggtaaaa cgatcccgag gtctaaatct tcaaaaaagc ggatctcgtt
    14041 cacagattga tggaaagcta tccattcgca aacttgtttg gcatgaagga aagattgcgg
    14101 tgtctcttgc tctgtataac aaagcgaccc aattccaaag tttagctgta actgatacat
    14161 tttattaaac tttgtctgga ttttatcaag caatgatagc aatgaggctc tggaaatccg
    14221 attgactagg ataatgattt gttgtctatg tacagtgagc aaagcctgtc cgtaataaga
    14281 gaagaccctt tcgagcaact ccaataaagg attgatccgt tcttgacaca attgctgatt
    14341 gtcagaaagt tgtcccacaa ctacataata tggagaagaa aaatcaagct caattaagtg
    14401 agggtggtat cgactatgta cttgatatca tcaatgaacc gatggtactt tcttatgcga
    14461 tggaacaggt agagcgattg cttgtttgtg caggaaaaca agcgatgcgg ctgatacgat
    14521 taggtgagaa aataagaaga taaaaataca cggtcccttc atggcgaccg tgcatttttg
    14581 gtttttatta aatatttaat actttattca agaaatcttt tgtccgttca ttttgcggat
    14641 tttcaaagat ttgttgaggc gttccatctt ctaaaaagtt tccgtcgtca atgaacatga
    14701 cgcgattggc cacttcttta gcgaacccca tttcgtgcgt aacgataacc atcgtcatac
    14761 cttgttctgc taactttttc atgacgttca agacatcacc gaccatttca ggatcgagtg
    14821 cagaagtagg ctcatcaaaa agcatgatgt cagggttcat cgctagagca cgagcaatag
    14881 ccacacgctg tttttgtcca ccagaaagca tttcaggata catatctttt ttatcttcta
    14941 gaccaactgt ctgcagtaat ttgattgctt ttttctctgc ttcttctcta gattctttct
    15001 tcaggtccat cggtgctaaa atgatgtttt ccaaaataga aagatgaggg aataggttaa
    15061 agtgttggaa taccattccg atatgttgtc tcaccaagtt gatatctgtg cttttatcca
    15121 tcaagtgggc gccgtcaata ataatttcac cgtcagaagg ttcttctaaa cgattcaagc
    15181 aacgcaagaa tgtacttttt cctgaacctg aagggccaat gatacagacg acatctcctt
    15241 cgttgatgga tacattgatg tcgtttaata ctgtgttatc gccgtatttt ttgactaaat
    15301 gctcaactaa gattttttct gccatcttac ttcaccttct tttctaaaac ttttgctaat
    15361 tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa tgatcaagta aacatacgta
    15421 ctttgtgttg tacgtgcaac aatgatctta ccagtttgca gcaattcgac tacaccgatt
    15481 gcggaaatga tcgttgtgtc ttttaatgag ataacgaact gattgacgaa tgatgggatc
    15541 atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg tccgattata agaaagacct
    15601 aaacttctgg aagcttccat ttgtccgaca ggaacggcgt tgatcccgcc tcgaacaatc
    15661 tctgcgatat aagcacttgc gttaagagtc aaggtgataa caccagcaag gaagtcagga
    15721 atcgtaaatc caacgatacc aggcaaccca aagaaaataa agaacgcaag aaccatcatt
    15781 ggaattcctc ggatgatatc gacataaata ctagcaattg tgcgaagagc gcgtattggt
    15841 gctacactga ataatccaaa aatcacaccg attactatag ccaaagcaaa ggagatcaaa
    15901 gttaggagaa ttgttctcca aagtcctttc aacaatgctt gatagttgtt ttggataagt
    15961 ccaagaaatg ttgattcatc agcagtggaa gactcactgg aagtttctga accatccgct
    16021 acatattctt taataatctt atcgtattcg cctgttcttt ttaattcttt caatccttca
    16081 ttgaacatat taagaagctc aggattttgt ccttttttaa cggcaaatgc atattttccg
    16141 ccagattctt ttttgatcgg tgtttccaat tcttgtcctt gtttgatcgc ataaccgatt
    16201 accggataat catccatcat tgcatcgatt gcgccaactt ttaacccgtc gtacaattga
    16261 tcagcagcat caaaatattt gatagaatag ccatattcag attcatggtc gcttaaaaat
    16321 tcagcacttt cggtaccaac ttttgctccg actgtttttc ctttcaaatc ttcataagac
    16381 ttgatcgaag tatctccttt tttgacagct aattggatcc cgctatcaaa gtaagaatca
    16441 gaaaaatcat agcttttctt tcgttcatct gttacagtca ttccggcaat cattccgtct
    16501 gcttgtcctg attccaaagc ttgcaaagca gcagagaaac cacggtagtc cattcggatg
    16561 ttaaatcctt gcagtttggc gatgttttcc atcaatttta catcgatgcc ttcgtactcg
    16621 ccctttgcgt tttgaaattc aaatggagca aaagcagagt cactggcaat tacgtatacg
    16681 tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc ttgactctgc cgttgtagtg
    16741 ttgtcagctt gtgcaacatg accaaacgca agtgtcacaa gtcctgtcat taagaaaaca
    16801 aatgagtgaa ataaatgctt tcttttcata atcaacctcc taaatttcca atattttatt
    16861 attataggcg aaaagtgctg aatcagacaa gactgtaagc tcgaaaaaat gaaaaaaatg
    16921 cgagtttcta acattttatg ttataaaaca taacatgtat aataagataa aaaagtggcg
    16981 aaagtatgtt cggtgtggta taatttttta gtaaggaatg cctttgcttt ccttttcttt
    17041 ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg acgaagaatg attgagagag
    17101 atacctcccg acactttgag cttgtctcga aataccaacc ggctggtgat cagcctgaag
    17161 cgattaacca attagtggat ggcgtagtcg gcgggaaaaa agcacaaatc ttattaggtg
    17221 cgactggaac agggaaaaca tatacgattt ccaatcttat tgagaaagtg aataaaccaa
    17281 cactgattat tgcccataac aaaacattgg ctggtcagct gtacggggaa ttcaaggaat
    17341 ttttccctaa caatgctgtt gaatattttg tttcttatta tgattattat cagcctgaag
    17401 cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc aagtgtaaat gatgagatcg
    17461 acaaattacg acattcggct actagttcat tattagaaag aaatgatgtg atcgtcattg
    17521 catccgtttc atgtattttt ggattaggct cgccttttga gtaccaaaaa caagtagttt
    17581 cgattcgaca gggagcagaa ctagatcgca atcagttgat tcgtgactta gtcagtatcc
    17641 aattcgaaag aaatgatata gattttcagc gcggtcgttt tcgagtgcgc ggtgatgtcg
    17701 tggagatttt tccggcttca agagatgaac gtgccttacg tgtggagttt tttggagatg
    17761 aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga agttttagga gaaacggaac
    17821 acgtagcaat cttcccggcg actcactttg tgacgaatga tgaacatatg gagcatgccg
    17881 tagcgaatat caaagcggaa ttagaacagc gtttaactgt cttgcgaaat gaaaacaaac
    17941 ttctggaagc tcaacgttta gaacagcgaa ccaattacga tattgaaatg atgttagaaa
    18001 tgggctacac gtcgggaatt gaaaattatt ctcggcatat ggacggacgt aaagaaggtg
    18061 aaccgcctta tacgctattg gatttctttc ctgaagattt tttgatcgtg gcggacgaat
    18121 cgcacgtaac catgcctcaa atcagaggaa tgtataatgg tgaccgagcg agaaaacaga
    18181 tgctagtaga ttacggtttc cgtttgccat ctgctttaga caatcggcca cttcggttag
    18241 aagagtttga aaaacatgtc aatcagatca tttatgtctc tgcgacgcca ggaccatatg
    18301 aacatgaaca aacagatacc gttatccaac aaatcatccg tccgacaggg ctgcttgacc
    18361 cagtgatcga agttcgtcca atcatgggac aaatcgatga tctagtaggc gaaataaatg
    18421 aacgagtcga aaaagaccag cgggtgtttg taacgacatt gacgaaaaaa atggcagaag
    18481 acctcacaga ttatttcaaa gaattaggga tcaaagtcaa atatttgcat agtgatatca
    18541 aaacgctgga acgaacagaa atcattcgag atcttcgatt aggtgaattt gatgtgttag
    18601 ttggaatcaa tttgcttcgt gaaggattag acgtacctga agtttccctt gttgctatct
    18661 tggatgcgga taaagaaggc tttttacgta gtgaacgttc cttagtccaa acgattggac
    18721 gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc agacaaagta acagattcta
    18781 tgcgtctagc gatggatgaa acgtcaagac gtcgtacgat tcagcagaag tacaatgaag
    18841 aacatgggat cgttccaaaa acgatcatta aagaaatccg agatttgatt tctattacga
    18901 aagaatcgga agatgataca aaagaagcgg tacaagtctc gtacgaagaa atgacgaagg
    18961 aagaaaaaga cacgttattg atgaaattag aaaaagaaat gaaagatgcg gcaaaagctt
    19021 tggattttga aacagccgcg aatgtgcgag atatgatttt agaattaaaa gcttcaaagt
    19081 aggaggacaa catggctaac gataaaatta cgattcatgg tgcacgtgcc cataacttga
    19141 aaaatataga tgtaacgatt ccacgagata aattcgtggt cgttacaggt ttgtccggtt
    19201 ctggaaaaag ttccctagca tttgatacgc tgtacgctga agggcagcgg agatacgtag
    19261 agagcctttc tgcttatgct cgtcagtttt taggacaaat ggataagccg gacgtagata
    19321 gcatcgacgg attgagtccg gcgatttcta ttgatcagaa aacaacaagt aaaaatcctc
    19381 ggtcgacagt aggtactgtg acggaaatca atgactattt acgcttgttg tttgcacgtg
    19441 taggacatcc gatttgtccc aacgatcata tcgagattac cagtcaatca gtagatcaaa
    19501 tggcagatca agtaatggct ttgcctgagc gcacaaagat ccaagtgttg gcaccgatcg
    19561 tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg aatccaaaaa gagggatacg
    19621 tccgcgttcg agtcgatggt gagatctatg atttaagtga agtgcctgag ctggagaaaa
    19681 acaaaaaaca tgatatcgcg atcgtcatcg accggatcat tgtgaaagaa gggattcgtt
    19741 ctcgcctatt tgattcttta gaggcggccc tgcgattagc agacggctat gctctggttg
    19801 acgtgatcgg tgaagaagaa atgttgttta gcgaacacta tgcctgtcct tattgcggat
    19861 ttactgttgg cgaactagaa ccaagactgt tctcattcaa tgcgcctttt ggagcttgtc
    19921 cagactgtga tggtttaggt gtcaaattag aagtcgatca agatttagtg attccagata
    19981 gaacgaagac acttagtcaa ggtgctatcg ttccatggaa tccaatcagc tctcagtatt
    20041 atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat cgatttagat acaccattcg
    20101 aagaattacc ggaagagcat caggagatca tcttgaatgg gacagatgat cttttccatt
    20161 tccattatga aaatgatttt ggtggcgttc gcgatgtaga aacgactttc gaaggggtat
    20221 tgaagaatat cgagcgtcgt tatcatgata cgaacagtga tttcacgcgt gagcaaatgc
    20281 gtctttatat gacagaatta acttgtcaga catgtaaagg ttatcgattg aatccccaag
    20341 ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac aaatgagttg tctattcaaa
    20401 aagcagtaga tttcttctca aacttaacgt tatctgaaca agagaaagta attgccaaac
    20461 cgattttaaa agaagtaaat gacagacttt cattcttgga aaatgtggga ctagactatc
    20521 taactttgag tcgcgcttcc ggaacattat ctgggggaga agcgcagcga atcagattgg
    20581 ctacacaaat tggatcgaat ctttctggag ttttgtatat tttagatgaa ccatcgatcg
    20641 gattgcatca acgcgacaac gatcgactga ttggctcatt aaagaaaatg cgtgatcttg
    20701 gcaacaccct cgttgtagtc gaacatgatg aagatacgat gtgggcagca gattatttga
    20761 ttgacgtcgg acctggggcc ggagaacaag gcggacaaat cgtggctgca ggcactcctg
    20821 aagaagtagc taatgatgaa aattctttga cagggaaata tctttcaggg aaaaaatcaa
    20881 tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc aatcaagatc actggtgctt
    20941 cagaaaacaa tctgaaaaat attagtgttg aatttccatt aggagaattt gtagcagtca
    21001 caggtgtgtc tggttctgga aagagtacat tggtcaatag tatcttaaag aaatcgttag
    21061 cgcaaaaatt aaataagaat tctgctaagc caggtaaatt caagacaatt tccggctacg
    21121 aaagtatcga aaagatcatc gatatcgatc aaagcccaat cggccggacg ccgagaagta
    21181 atccagcgac ttatacaagt gtatttgatg atatccgtgg gttatttgct caaacgaacg
    21241 aggcaaaaat gcggggttat aagaaagggc gttttagttt caacgtaaaa ggcggtcgtt
    21301 gtgaagcttg tcgcggggat ggaattatta agatcgaaat gcactttttg cctgatgtct
    21361 atgttccttg tgaagtatgt catggtaaac gatataattc tgaaacatta gaagtgcatt
    21421 acaaaggaaa aagcattgct gatattttgg aaatgacagt agaggatgct gtagaattct
    21481 tcaagcacat tccaaagatt catcgcaaac tgcaaacgat tgttgatgtt ggcttaggtt
    21541 atgtgacgat ggggcaacca gcaacgacat tgtccggtgg tgaggcacaa cggatgaaac
    21601 ttgccagtga attgcacaaa atctctaatg gaaagaattt ctatatacta gatgaaccaa
    21661 cgacaggact tcatagcgat gacatcgccc gcttgttgca tgtattacaa agattagtag
    21721 atgctggtaa cacagtttta gtgattgaac acaatctaga tgtaatcaaa acagcagatt
    21781 atatcattga tttaggacca gaaggtggag aaggtggagg aacgatcctt gcgactggaa
    21841 caccagaaga aatcattaac gtaaaagaaa gttatacagg tcactatttg aaaaaaataa
    21901 tggtataaag aggatagata gctgagatag ttaaaaaaac ataaaaaaat gaatttttgc
    21961 ttgttttatt aatatcagtg ttctagaatc attacatggt agttctttct acactgattg
    22021 gtatagataa aaacttccta taagttcaat ccgttcaaaa aagaattgag caatcggagg
    22081 actttccttt gattgctttt ttctttatgg tatgatattc cttgtgtagt tggaaaagaa
    22141 aaacaagaga ttatgagtaa gtgagagata atgatgaaca gtattgaaaa tgaagaattt
    22201 atccctatat tattaggaag cgacatgaac gtttatggta tggcacgttc ttttaatgaa
    22261 gcatacggca aaatctgcca agcatatgct tcggatcagt tagcccctac ccgctacagt
    22321 aaaattgtca atgtagaagt tattccagga tttgataaag accctgtatt tatcgagacg
    22381 atgcttcgtt tggcaaaaga aagatattct gataagagta aaaaatatct tttgattgct
    22441 tgtggtgatg gatatgcaga attgatctcc caacataaac aagagctgtc agaatatttt
    22501 atctgtccat atattgatta ttctttattt gaaaggctga tcaataaagt aagcttttat
    22561 gaagtttgtg aagaatatga tcttccgtat cctaaaacat tgatcgtcag agaagaaatg
    22621 ttagtaaatg gacatttgga acaggaattg ccttttgaat tcccagttgc tttgaaacct
    22681 gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa gaaaaaaagc ttttatttta
    22741 gagactagag aagagttcga tttaatttta ggccggatat acgaagccgg ttataaaagt
    22801 gaaatgattg tacaagattt tatcccgggt gatgatagta atatgcgtgt gctaaatgcg
    22861 tatgtagatg aagatcatca agttcggatg atgtgccttg gtcatccact attagaagat
    22921 cctacaccag catcgattgg taattacgtt gtgatcatgc cagattataa cgaaaaaatc
    22981 tatcaaacca ttaaggcatt tttggaaaaa atcgaatata caggctttgc caatttcgat
    23041 atgaagtatg atcctagaga tggagaatat aaattatttg aaatcaattt gagacaaggt
    23101 cgtagcagtt tctttgtgac attgaacggc ttgaatctgg ctcgttttgt cacagaagat
    23161 cgagtgttca acaaaccctt tgttgaaacg acctatggaa cgaatcaatc agataaagca
    23221 agattatgga tgggtgtgcc caaaaaaatc tttttagaat atgctcggga aaatgaagac
    23281 aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg gtactactgt attttatgaa
    23341 aaagaccgat caatcaagcg ctggttgttg atgaaatata tgttccataa ctatattcca
    23401 agattcaaaa agtattttca tgtgaaagaa ggctagttca tggagaattt tttcagtatt
    23461 ttaggcggaa tgggcacgat ggcgacagaa agttttgttc gcttgatcaa ccaccgaacg
    23521 aaagctacaa aagatcaaga atatttgaat tatgtcttat tcaatcatgc aacagttcct
    23581 gatcgtacag cttatatttt agatcgatca gaagaaaatc caatgccgtt tctgctggat
    23641 gatattgaga aacaaaattt attgcgacca aacttcattg tgttgacttg taacaccgca
    23701 cactactttt ttgaagagtt acaagcggca acggatattc ctatcttgca tatgccaaga
    23761 gaagcagcaa atgaacttgt ccgtcagcat acgacaggga gagtagcgat tctaggaaca
    23821 gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca aaaatcttgg tttcgaaaca
    23881 gtgattcctg atactgcgct tcaagaaaaa atcaattacc tgatctatca tgagatcaag
    23941 gaatcggatt atttgaatca agagctctat tacgaaatct tagaggaagc ggtagagcgc
    24001 ctaaactgtg aaaaagtcat cctaggttgc acagaattat ctttgatgca tgaatttgct
    24061 gaagataatc attacccagt gatcgatgct caatctattt tagcggatcg aacgatcgaa
    24121 cgtgcattag ctgaaagaag cgaggcattg gatacagcat cggaaaagta aaaaaattgg
    24181 atttggggca aatatcctaa aagatatttg gtccaaatct ttttgtaaaa gaaaaagaaa
    24241 actggtaatg atacgcccgg gatgatataa ttttattatg cacaatgtga gaggggttcg
    24301 taaatatgac agataatttg caattagtca tcataacagg tatgagtggt gcagggaaaa
    24361 cagtagctat ccaaagtttt gaagatatgg gctatttttg tatcgataat atgccgcctc
    24421 gattgattcc aaaattctgg gaattgatca aagaatcagg taaagtcaca aaaatggctt
    24481 taggagtgga tttgcgttct cgttccttct ttgaagaaat ccaaaacatg ctgatcgaaa
    24541 tcgaaaatac gaacttcatc gacacttcta tcttattttt agatgcttca gacgaagaac
    24601 tagtggctcg ctacaaagaa acaagacgag cacatccaat ggcgatggac ggtttgatca
    24661 ctgaagggat acgtaaagaa cgggcgatac tagacgactt gaaagcccaa gcttccgtga
    24721 tcattgatac gacaacattg acaccacgac aattacgtga aaaaatcaat gagtcattca
    24781 aatcaaaggg agaccaagga tttcggatcg aatttgtatc atttggtttt aaatacgggt
    24841 tgccgatcga ttcggatatt gtgatggatg tgcgtttttt gcccaaccct cattatatcc
    24901 cggaactaag accattgacc ggaaaagatc agccagtcta tgattatgtg atgtcctttg
    24961 atgaaacgca atcattctac cataaatttt tggatcttct ggaaactgta ttgcctggat
    25021 acattaaaga aggaaaaagc agcttgacag ttgcaatcgg ctgtacaggc ggtcaacacc
    25081 gttctgtggc attgactgaa cgtttggcaa atgctttagg gaaaaactat aaagtcaatg
    25141 tgacacatcg ggacaaggat aaaaggaaag agacggtgaa ccggtcgtga aaatgaaaac
    25201 ctaccggata agaaaaccaa agatcgtagt ggtcggcggt ggcactggtc tgccagttat
    25261 cctgaaaagt ttacgaaatc aaggagcaga tatcacagca gtagtgactg ttgcagatga
    25321 tggtggaagc agcggtgcaa tcagagaatc gatcgctatg gctcctcctg gtgacttgag
    25381 aaatgtctta gtggcgttat cggatatgcc acaattctat gaagatattt tccaataccg
    25441 tttcaaaaag gaagatcagt ttttagccaa tcatacgatc ggtaatctga tcattgccgc
    25501 agtttccgag atgcggggaa gcacttatga ggcgattcaa ttattagcaa aaatgatgca
    25561 tgtagatgga catgtttatc cttcttcaga aactcctttg accttgcatg ctgtttttaa
    25621 agacggaacg actgcagtag gagaatctaa aatcgcgatc aaccgtaaaa ccattgaccg
    25681 tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa gcggctcgaa aagtcgtttc
    25741 agcaattatg gacgccgaca tggtcgtttt gggacccgga agtttattca ccagtatcct
    25801 tccaaattta gtgataccag aaatcggcga agcaatgctc aagacaactg ctgaaaccgt
    25861 ctatatctgt aatatcatga cccaaaaagg ggaaacggaa catttcacag atgccgatca
    25921 tatacgtgtt ttacacaagc atttgaatca gccttttatc gatacagtat tggtcaatac
    25981 ggaaaaagtg cctgaagatt atatggaccc agagatctat gatgagtatc tagtacaagt
    26041 cgcacacgac ttcaacggat tgagaaatga aggatgtcga gtgatttcca ccgacttttt
    26101 agaattgaaa gatggtggtg tcttccacga tggagacaaa gtagtagaag aattgttccg
    26161 tttagttttc ggttcaaaat attaagcaat ttattgttgg agaaaggagg agaataggat
    26221 gtcatttgct gcagatgtga agaaagaact aactggtttg gaagttcatc gagaacatgc
    26281 gaaagcggaa ttagctgctt tgctgcgaat gaacggctct ttaggtattg tgaaccagca
    26341 gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc agacgtatct attctctttt
    26401 gaaagatcat tatcaggtac gaagtgagct actcgttcgt aaaaaaatga aattaaaaaa
    26461 gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa gatgtgttga tggatcttga
    26521 tatcatggat ggtatgatgt tcaatggaca cgtttcggac gagattatgg gaaattcgca
    26581 aaaaatgcgt tcttacttac gtggtgcttt tatggcctca ggttcagtca ataatccgga
    26641 aacgagtcgt tatcatttgg aaatattttc tatttatgaa cagcataacc aagacatttg
    26701 cgatatgtta aattattacg gtctacatgc tcgaacgctt gaacggcgca atggctatat
    26761 ttcgtattta aaaggagcag aaaaaattgc tgatttctta acattgattg gtgcaaccaa
    26821 ctcaatgctc cgatttgaag atgttcgaat cgtacgggat atgagaaatt cggtcaatcg
    26881 tctagtcaat tgtgagacag caaatctgaa caagacgatt gatgcggctt ctaaacaaat
    26941 cgaaaacatc gaattgatcg aagcaaaggt cggtctacat gcccttcctg aaaaacttca
    27001 ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc ttgaaagaat taggggaaat
    27061 gattccctca ggtgcaatat caaaatcggg aattaatcac cgaattcgca aaataaatga
    27121 atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa gaaaaaactg atacatgaag
    27181 caggagatgc gaatgtatca gtttttttta aaagctatac ttgcttttct tccgtcaaac
    27241 tataattcca actttactgg agtaacataa tgttgaaagg cttctattgt tttttaatac
    27301 aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttatac ataaactttt
    27361 gcttaatata gaagtcctga tatagatagg atcaagtatt tatctataac tggagattaa
    27421 ttaaaaactg gaaggaactg ctcgatcaac cttgagaact gtttttctta cttctggtca
    27481 agattctcgt ggttggtgga taaggatggt tgataagttc attggattag ctagtcgaaa
    27541 acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt tattcagtga
    27601 taatcataag ttcataaata gtaattggga tgtatggttg gtatacgtta tacctgaact
    27661 tgaactatca agatttggtt tagaatttga gaatcgatgt ttataaaggg agggcaaaaa
    27721 atgtgctttt ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat
    27781 atagcaaagc gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata
    27841 acatttatat cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact
    27901 acagcaccac cattgccaga gattacatat ggagaatttc cgtttagtct tgaatatgaa
    27961 atagatgagg aaagaatttt gattgaagat acggtgattg cagaatttat aggaagtttt
    28021 agagggaatg tgacaactca acctagacga agttggagta taaaactttt aaataatagt
    28081 ggagagttga aagcatttca ttatgaattc ttaataatag aaagtgaaga tgtaacaatc
    28141 actttaagta gtggctctgc tggatatttt atgaatgatg gtggaagcaa gccacagctc
    28201 acagtccgtg atcgtattgc aggttttatg catttttctc actatgactt agaagaagct
    28261 cgtaaaatac ttgcagaata tgggatcaca ttaatcaatt gggaatatgc gccaccgata
    28321 agaaattttt tcaatgatta ttattagtct tataaaaaac agaatgtaaa atttatgtgc
    28381 gcgagtattt agttttccat tttttgtttg atagatatat agcattgttg aagcatggga
    28441 tagtggtgcc aattttatca atgaggttgg tcaggcagtt aacataattg gtgatgctat
    28501 aaatccattt aattggaatt ggaggaatta ggtgtggaaa cagtgagatt atggatattc
    28561 atgaaaaatt ttcgttcggc tttgagatat gttattctgg aatcaaaatc aggttattat
    28621 ctgattgaca cagatcacta tactttttgg ggaggctacc tttttccgat ttttaattgg
    28681 ataatccccc ataaggttat gaaaattaac ttgcctgcag aagaaataaa tagattaata
    28741 ctcaacaaag atttacaaag gaaaagagag aaagaacatt ctaatgttgc ttggataggt
    28801 ccagcatttg cagtagtgac ttcttcaatt gtcggtccta tgctagagag tatttatagt
    28861 gatttttcta ttggaatacg ttatttattt gttactttaa gtatagctgt tcttttgtgc
    28921 ataaagggtt atgttagcaa gaagaataaa gatattttaa cactaatcgg tgtaaacaat
    28981 ttatctagta ctaaaattat actgtttcct gaatctataa aagaaggatc aaggttgttg
    29041 ataattttta taatgcttat actgttttct gttttatttg gggtgatgtt tataga
//
LOCUS       DACCXM010000020        28974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCXM010000020 DACCXM010000000
VERSION     DACCXM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.021"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..756
                     /locus_tag="IVY61_000782"
     CDS             <1..756
                     /locus_tag="IVY61_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP7969917.1"
                     /translation="ENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVLK
                     ILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETIH
                     LGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLEK
                     HPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAFS
                     VSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            856..1500
                     /locus_tag="IVY61_000783"
     CDS             856..1500
                     /locus_tag="IVY61_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP7969918.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            1507..2514
                     /locus_tag="IVY61_000784"
     CDS             1507..2514
                     /locus_tag="IVY61_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP7969919.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            2530..3372
                     /gene="kduI"
                     /locus_tag="IVY61_000785"
     CDS             2530..3372
                     /gene="kduI"
                     /locus_tag="IVY61_000785"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP7969920.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            3392..4195
                     /locus_tag="IVY61_000786"
     CDS             3392..4195
                     /locus_tag="IVY61_000786"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP7969921.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(4444..5508)
                     /locus_tag="IVY61_000787"
     CDS             complement(4444..5508)
                     /locus_tag="IVY61_000787"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP7969922.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(5505..6590)
                     /locus_tag="IVY61_000788"
     CDS             complement(5505..6590)
                     /locus_tag="IVY61_000788"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP7969923.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(6603..7580)
                     /gene="mvaD"
                     /locus_tag="IVY61_000789"
     CDS             complement(6603..7580)
                     /gene="mvaD"
                     /locus_tag="IVY61_000789"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP7969924.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(7573..8517)
                     /gene="mvk"
                     /locus_tag="IVY61_000790"
     CDS             complement(7573..8517)
                     /gene="mvk"
                     /locus_tag="IVY61_000790"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP7969925.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            8839..9492
                     /locus_tag="IVY61_000791"
     CDS             8839..9492
                     /locus_tag="IVY61_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7969926.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            9577..10482
                     /locus_tag="IVY61_000792"
     CDS             9577..10482
                     /locus_tag="IVY61_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7969927.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(10484..11116)
                     /locus_tag="IVY61_000793"
     CDS             complement(10484..11116)
                     /locus_tag="IVY61_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7969928.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            11436..11960
                     /gene="infC"
                     /locus_tag="IVY61_000794"
     CDS             11436..11960
                     /gene="infC"
                     /locus_tag="IVY61_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP7969929.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            12032..12232
                     /gene="rpmI"
                     /locus_tag="IVY61_000795"
     CDS             12032..12232
                     /gene="rpmI"
                     /locus_tag="IVY61_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP7969930.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            12285..12644
                     /gene="rplT"
                     /locus_tag="IVY61_000796"
     CDS             12285..12644
                     /gene="rplT"
                     /locus_tag="IVY61_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP7969931.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            12896..14233
                     /locus_tag="IVY61_000797"
     CDS             12896..14233
                     /locus_tag="IVY61_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7969932.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            14281..16410
                     /locus_tag="IVY61_000798"
     CDS             14281..16410
                     /locus_tag="IVY61_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP7969933.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            16385..17074
                     /locus_tag="IVY61_000799"
     CDS             16385..17074
                     /locus_tag="IVY61_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969934.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(17482..18159)
                     /locus_tag="IVY61_000800"
     CDS             complement(17482..18159)
                     /locus_tag="IVY61_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7969935.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(18295..18489)
                     /locus_tag="IVY61_000801"
     CDS             complement(18295..18489)
                     /locus_tag="IVY61_000801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969936.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(18539..18979)
                     /locus_tag="IVY61_000802"
     CDS             complement(18539..18979)
                     /locus_tag="IVY61_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP7969937.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(18983..19828)
                     /locus_tag="IVY61_000803"
     CDS             complement(18983..19828)
                     /locus_tag="IVY61_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP7969938.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            19977..21395
                     /gene="pepV"
                     /locus_tag="IVY61_000804"
     CDS             19977..21395
                     /gene="pepV"
                     /locus_tag="IVY61_000804"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP7969939.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(21452..22399)
                     /locus_tag="IVY61_000805"
     CDS             complement(21452..22399)
                     /locus_tag="IVY61_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7969940.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(22622..22972)
                     /locus_tag="IVY61_000806"
     CDS             complement(22622..22972)
                     /locus_tag="IVY61_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7969941.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            23172..24251
                     /gene="pepA"
                     /locus_tag="IVY61_000807"
     CDS             23172..24251
                     /gene="pepA"
                     /locus_tag="IVY61_000807"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP7969942.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24395..24715
                     /locus_tag="IVY61_000808"
     CDS             24395..24715
                     /locus_tag="IVY61_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7969943.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            24737..25201
                     /locus_tag="IVY61_000809"
     CDS             24737..25201
                     /locus_tag="IVY61_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7969944.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25406..26011
                     /locus_tag="IVY61_000810"
     CDS             25406..26011
                     /locus_tag="IVY61_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP7969945.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(26050..27339)
                     /locus_tag="IVY61_000811"
     CDS             complement(26050..27339)
                     /locus_tag="IVY61_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7969946.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            27767..28246
                     /gene="rlmH"
                     /locus_tag="IVY61_000812"
     CDS             27767..28246
                     /gene="rlmH"
                     /locus_tag="IVY61_000812"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP7969947.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28449..28974
                     /locus_tag="IVY61_000813"
                     /pseudo
     CDS             28449..28974
                     /locus_tag="IVY61_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177294.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         9136 a         5411 c         5995 g         8432 t
ORIGIN      
        1 gaaaataaaa agccttatgg aactgtattg atcaaagcct ctaaaatact agactattta
       61 gcggaaaacc aagatgtctc tttgcagacg atcgcaaagg gtgtgggaat gacctcttct
      121 acagtcttga aaatactaga tactttgttg atgatcggct acgtaaataa aaattcagag
      181 aaaaactatc gtttaggtgc aaagcttatt cgctacgcga ataaaaacat tgaacaaatc
      241 gatttggtag agttgacttt accgttttta gaacagctgc agcaaaagat cgatgagact
      301 atccacttag gggtattgga caacaatgag atcttgtacg tgaacaaact agatccaaaa
      361 catcaaacga tccgcatgtc ttcaaaaatc ggaatcaccc gaccattata caattctgcg
      421 atgggcaaag ccgtattagc agaattcagt gaagagcagt acactgatta tcttgaaaag
      481 catccgttga tcccttacac agaatatacg atcaccaatg cattacggtt gaaaaaggaa
      541 atcaaaacgg taaaagaaac ccacatcgca tttgacgatg aagaaatcga acgtgatatt
      601 ttttgtatag gagcctcaat cgtgaaaaac gaacaaatca ttggtgcatt tagtgtaagt
      661 atgccgaaat atcgtttaac agatgagaca aaagaacaaa taatcagtgc cctcaagcaa
      721 acaaaaagcg aaatcgagca aagaattcaa aaataatgaa aaaagcgttt gcaatttttc
      781 ttttctgccg tataatatgg gtatagattt ccaagttata aaataaaatt tctaaatagg
      841 aaataggtgg agaagatgaa aaaaattgat attttatctc gattgaaaaa cgccggagtg
      901 attgcggtgg tacgcggaaa aagcaaagaa gaagctttga atgcttgcca cgcaatcatc
      961 aaaggtggtc taactggtat tgagttgact tttacagtcc ctcaagcaga tcaagtaatc
     1021 aaagaacttt tgtcattcta caaagaccaa ccagaaatag tgattggcgc aggcacagta
     1081 ttagacgctg tcacagctcg cttggctatc ttagctggcg cagagtatat tgttagccct
     1141 tcctttgatc aagaaacggc agagatgtgc aatctgtatc aaattcctta cttaccagga
     1201 tgtatgacta ttaccgagat caaaacagcg ctgaaaagcg gggtagatat cgtcaaacta
     1261 tttcctggaa gcgcttatgg accaagtatc atttctgcct tcaaggcgcc gatgcctcaa
     1321 gtcaatatca tgccaactgg cggtgtgagt ctggacaata tgaaagagtg gttcgatgct
     1381 ggtgtcgtaa cagtcggagt aggtgggaac cttttagcac ccgcagctac aggagatttt
     1441 gacaaagtaa cggaagtcgc gcaacagtat gcagcaaaaa tgaaagaaat caagaggtag
     1501 ctgcttatgg gaaaagtagt gacactcgga gaaatcatgt tgcgattgtc cacagagcag
     1561 ggaacccgta tcgattcaag tgaacgattc gctgcccatt atggcggagg ggaagcaaac
     1621 gtagcaattt ctttagctaa ttacggacat gaagcgattt ttgctacaaa agtacctgat
     1681 aacgcattgg gaaaagcagt caaaaagcac ttgcaatgtt atggagtaga tacgaaacag
     1741 gtattatctg gtggatcaag actaggtact tattatatgg aaactggtag tggcgaacgc
     1801 gccgcctcag tgatctatga tcgtgccgga tcaagttttg ctgaaatgga tcatttggaa
     1861 tggtcgctag aggagttatt ccaaggaata gatatatttc atgtttcagg aatcacccca
     1921 gctttgtcta aaaaatggca aaaaatgacg aaacaggtga tagaagctgc aaaaaaagct
     1981 ggctgcctga tcagcttcga tatcaactat cgtggaaaat tatggacaca gcaagaagcc
     2041 agcgaagtaa tccatcagct tttaccttta gtagatatct gctcagctgg aaaaatggat
     2101 gctctttatt tgttaggtat cgagaaagcg ccagaagcag aagaacaacc tctaatttat
     2161 tattatcaaa aaatgcagga aaagttcccg aatattcagg tgttttattc gaccaagcgt
     2221 accgttcatt cagcaagtga aaatgaattg aaggggacat tgtggatgaa ccatgaatat
     2281 gtggaatccc aagtccatag aatcacccca atcgtggatc gagtaggtgg cggagatgct
     2341 tttgcaggtg gcgtgctcca tgggatttta tccaatatgt ctccgcaaga aatcattgat
     2401 tttgctacgg cagcttctgc cttgaagcat acgatccatg gggactgcaa ccaatttagt
     2461 caagaagaag tcgcacaatt tttagcatgc ggctctggaa aaatcattcg atagaaggag
     2521 tagaagaaaa tgcaagaaat ggaaacacgt tatacacaca gtccagaaga tatccgtcat
     2581 tattcaacgg aacaattgag aaaagaattt ttagtagaaa aggtatttga accagggaaa
     2641 atcagtttga cttatacgca caacgatcga atgatctttg gtggagtgac acctactgaa
     2701 aaagaattag aaatcacatt agacaaagaa ctaggtgttg attacttctt agagcgtcgt
     2761 gaactaggtg tgatcaatat cggtggacca ggattcatcg agatcgatgg gcaaaaagaa
     2821 gaaatgaaaa aacaagacgg atactatatc ggaaaagaaa cacgacatgt tgtattttct
     2881 tctcaagatg ctgaaaaccc agctaaattc tatattagtt ctgttccagc tcatcataaa
     2941 tatccaaatg taaaaatcag tatcgaccaa gtaaaaccaa tggagactgg tgaaggcctg
     3001 acactgaatg aacgtaaaat ctaccaatac atccatccaa atgtatgcga aagctgtcaa
     3061 ttacaaatgg gttatacgat tttagaacca ggaagcgctt ggaatacgat gccttgtcat
     3121 acacatgaac gtcgtatgga agcttatgtc tactttgatt atgccaatga agatacacgt
     3181 gtgttccaca tgatgggcaa accagatgaa acaaaacatt tagttgtaga caatgaacaa
     3241 gccattattt cgccaagctg gtcgatccat tcaggtgtag gtacaagcaa ttactcattc
     3301 atctgggcaa tgtgcggaga aaatatcacc tatacagaca tggatatggt gcaaatggat
     3361 caattaaaat aagcataagg gagagaaaat gatggaattt agtatggata tgtttcgttt
     3421 agatggaaaa gtagcattag tcaccggtgc tgtctatggt attggatttg agattgcacg
     3481 ctctttagct acagcaggag caaaaatcgt atttaataac ttgaatcaag aatcagttga
     3541 tgaaggtata gcgcattata aagaagcagg catcgatgca aaaggttatg tctgcgatgt
     3601 gaccgatgaa gaagcggttc aagcgatggt tcaacagatc aaagaagatg taggttcagt
     3661 tgatattcta gtcaacaatg cagggatcat caaacggaca ccgatgatcg aaatggatgc
     3721 ggcagacttc cgccaagtaa tcgacgtgga tttaaatgcc ccattcatcg tttcaaaagc
     3781 agttattccg gatatgatcg aaaaaggcgg cggtaaaatc atcaatatct gctccatgat
     3841 gagtgaatta ggacgcgaaa cggtcagtgc ttatgctgcg gcaaaaggcg gattaaaaat
     3901 gctgacgaaa aacatcgctt ctgaatacgg tcaatacaat atccaatgta atggaattgg
     3961 accaggttat attgctacac cgcaaactgc tccattacgc gaaacacaag aaaacggtga
     4021 acgtcatcca ttcgatcaat ttatcgttgg acgtacgcca gcagcacgct ggggcgagcc
     4081 aatcgatttg gctggaccat ctgtcttctt ggcttctagc gcatctgatt tcgttaacgg
     4141 ccatgtcttg tatgtggacg gaggcatact tgcctatatc ggaaaacaac cttaaaagag
     4201 ctttagtaca ctgtttagtg gagaggttgt gaaatcagcg gattgaacga ataccgaagg
     4261 acagctgatt aaacatcatt tattcgaaaa aagaggctgg gacagaagca tttaactcca
     4321 agaaataaga aggaattcac gaaaattgct tttcaaattt ttgtgaattt cagcttattt
     4381 ccgaaggagt tgcttttact tccgccgttt gtacgtgttt agagcgtgag acaaaagtgt
     4441 tctttacttt tgtcccacgc tcttttctgt gcgtcttcca tacttcacta agtcgatccc
     4501 tcttgcttcg caccaatcct tcactggtcc ggaaaagatc agagattgtt ggtgtagggc
     4561 cgcggtattc gttgcgccga ccatagtata aagcagacga agctcttctt gccattgctt
     4621 catcagtata atagtttctt ctacgccgtg attcatcaga tgcgtcagta cggttcctga
     4681 agtacccacg gcatttgcgc cgagacaaag ggctttaaat atgtcatagg cattgcggat
     4741 acctccggag gctaggattt ccatagaagt gtctgcttca tttgcttcaa gcaatgaaga
     4801 gactgtcgat tgcccccagt cagctaaata actcagttcc cgcttggagc gacgggcgtt
     4861 ttcgatctgg gtgaagcttg ttccgcttcg accgctgata tcaactgtat gaactcctaa
     4921 agcagctaga tcattgagcg tttctcttgt catgccaaaa ccgacttctt ttacgatgag
     4981 ggggacgtca atggctgttt gagtttcttg aataagtact ttccaattgg taaaatcacg
     5041 gtctccctca ggcataacca actcttgagg tgcattcaaa tgaatctgta aggcatctgc
     5101 gtgaaacaat cggatagctt ctttggctct ttcgacagaa gtccctgcac cgatgttggc
     5161 aatgattttc ccgtgaggat attcttgtcg cataatcgta taagtatcgg ctaaagaagg
     5221 gactttcaag gcagcgctga ctgaaccagt ggcaatcatc aaatctgctt ctcttgcgac
     5281 gattgcaaga tcttggttga ttttttttgt tttttcgctt ccgccagtca tcgcattgat
     5341 ataaaatggg ctgcttagtg taaacccaac tgcctgtgta cttagatcga catcagaaac
     5401 tgctatttgc ggcaaagggt tatggatgat ccgtacgaag tcaaattcgt ttttttgttt
     5461 atcatgaaag gctttagcta gtgaaacatg ttcgtctttt cgattcatcg gttttccttt
     5521 ctttgatcgc tatagacatg taacggcagt ggagtgattt ctgctttttc ccatgcactc
     5581 attaaaggaa gaatgccaga tttctggtca acaattacga ttccgcaatc gcccccacct
     5641 gcaccagaag attttgcggc tccttcatac tgttcagcta aattacacaa tttgttcaaa
     5701 gcaggcgttt cgatgacgac cccagtgatt gcagataaat catgcagtaa ttgtcggttt
     5761 tttcgaatca tctgttggat caacgttaca ttattttctt taaacccttt gatcatttcg
     5821 ttgacacatt ctgtactatt ttttaagaaa agctgataag ccaccatttt atcttctctc
     5881 gaacggtgaa cttgatcgac caaatcagaa gtagaggcag ggctacccgt ccaaccaatc
     5941 aataaacgta aatcttcagg agcaatcaat ggctcaatgg atagacctgg ccaatccagg
     6001 gcaagtaact cactgataga atgctgagtt tcttgttctt ggagccaagg atggtcgaag
     6061 gttgagaaag cgatccagcc accataacag ctagcagcga tgtcgccgca ggaaccatta
     6121 tcttgaactg ctaaattggc tagtgctgca atcttgaaaa tctccaactg agataaattc
     6181 aacgcgtaaa aaacattcaa ggctttgacc gttgcaacgg tcacggcacc gctagaaccc
     6241 aatccatatt tccggccatt tgaactgtct aactcacttg ttactttcag atcataaaag
     6301 gataaaagaa tgtttttttc ttgtgcatac ttttcagtca agcgaatagc agcaaggata
     6361 taatgaaaag gattttcccg aatatctaaa actaattctc cgttgcgtct tgtccaacgt
     6421 acaggcatcc cactatattg tgcagattgg atacttccga cttttcgtgc agattctaca
     6481 gtgactgtca cgaattgatc gactgcagcg ataactgctg gatggcctgt ttcaacaact
     6541 gcatattctc cggcaatata gagtttgcct ggtgcagata cttcaatcat aaaaatctcc
     6601 ctttattcaa taatcgcaat tcctggacca gcatgtgcag taattacttg ttctttgcta
     6661 aataatttgg taaatgtttc ttgtacctca gataaatggc tgttttccac taaaactttg
     6721 acattagggc cagcatccat cgtaaagtag catggaatgc cttgctttct caattgtcgc
     6781 accgcatcca tcgcttttaa gctgtcagga gaccagtaag tgaatggagg ctgggcagct
     6841 aaagtcgttc catgcatttt tagtccgttt ctttccatcg tttctccgag aagttggaaa
     6901 tcttttgttt tgattgcttg tttcaattga tatagatccc cttcaacgga atctaaccag
     6961 ccttgataaa aactggatgt ttcgactgtc cgacgcatcc catttctgct ggacacttct
     7021 tttttctggt cgttgatcaa aacgaaaacc attgctaaat cgtcttcgaa agaatcggaa
     7081 gggactggct tagcgtaaga acttaagtcg tcatggcctt tttcccattc gacgaaacca
     7141 ccgaaaatac ttcggcaagc tgaaccagac ccgcgtcgag caaatcttga aagagagaga
     7201 tcatctaatc caagcttaag agcagtattg caagcgcctg ctaaagcagc tagcccgctg
     7261 gcagaggagg ctaatccagc tgcggtagga acgaaattct gactgattac tgaagctttt
     7321 agtgagaggc cggcttcttt acgaaaaagg tcaagaaact gactgacttt ttttgttgct
     7381 ttttcgtctt gcaaggtgcc atctagataa aattcatcta ccatatagct atcagaaaag
     7441 atgacttctg tctctgtata aaatgcatcc aatgttaacg aaaggctgtt gttcattgga
     7501 aggatgagtt cttcattttt cttaccccaa tatttgatta gagcaatgtt ggtatatgcg
     7561 cgtgctttgc ctttaaacat aggtatgtac tcctaatcct tgtatccaag tttcagcagc
     7621 acccgcatct tcaagtgctt gactgatttc ttgggctttt gtttttgttt gtgctaatgc
     7681 aatcatacag ccaccgcgtc caccgccagt taatttggca cccaatgccc cggtatcttt
     7741 tgctgtttgg atcaatagat taagaaaatc attgctgatt gtcagctttt ccagaagtga
     7801 ttgtgattca tccattgtct gtgccaacgt ttctggtgaa ttttcaatga ttgcttgttt
     7861 tgcttgcttc gtcaagtatc ctaatttttg gatcatttgt ccagtttcat gaggctgggt
     7921 ttcaaagagg tgagcgacat ctttgacggc ttctcttgtt tgtcctttga ttcctgtatc
     7981 agcgacaatc aaaaaagcat cgatgttcaa agaaaagtag tcgaaaggat gcccttttgt
     8041 aaaataaatc ggctgcaagc tgctagtagc ggctgcatcg attccactag gattaccatg
     8101 ggcgattttt tccgaaagct ggacattttc taatagtatt tcacgagaca aaggaaatgc
     8161 taagtaatca taaaaagcac gagtgactgc tgtggcgact gcagcgcttg atcccattcc
     8221 acgttcagca ggaatcgtgc tttcaatggt cagttgcaag tttttttgga tgtcatgctg
     8281 tttttttaag tgaatgaata attttttgat attttttaat gcatgaggag cttcggctaa
     8341 atttcctgaa tagtaggaag aaaccagttg atcgtccatt gcatccagct cttcaaggaa
     8401 tgcggtgact tttgttgcat agaaaggaaa ggcaatcgct ggttctccat aaacaaccgc
     8461 gtgctcgccc atcaatatga tctttccgct tgattctcct tggccatagt ttgccataat
     8521 ctaactcctt ctcacgtaaa agtgattttt aagcaaaaaa taaagcgccc aaaacgagtt
     8581 gcgctgcttc acgaattttt ttgtccacta agggtctcaa aaaacattcc tcctttattt
     8641 tactaaaacc atctcagaag taaaactaag aatacgtttt ctaaagaaaa aacacttttt
     8701 tcttaagaat tttttgactt tttttcttct ttgactagaa aaaaatacaa ttgacactac
     8761 tagatattgg gggtatattt aaaaaggttg gagttaaaca caatatttag tgatgaagtg
     8821 agctggaggt aaatgagcgt gatcgtttta gcaggtacaa ttggtgccgg aaaatcaagt
     8881 ttaacagaaa tgatggctga acactttgat agtcaggcat tttatgaatc gattgatgat
     8941 aatgaagtct tgcccttatt ttatgcaaac cctgaacagt atgctttttt gctgcaaatt
     9001 tattttttga ataaacgttt tgcaagcatc aaacaagcaa tgaaagatga caataatgta
     9061 ttggaccgtt cgatttatga agattcactg cttttccatc taaatgcaga tctaggaaga
     9121 gcaacagaaa cagaagttcg tgtgtatgat gaactgctgg aaaacatgat ggaagaactt
     9181 ccctatgctg cccataaaaa acatccggat ttattagttc atattcgtgt atcttttgat
     9241 acgatgttgg agcgtatcga aaaacgtggt cgttcgtatg aacaattgtc ctttgatcca
     9301 agtctatacg attattacaa agaattgaac agacgttacg atcaatggta tgaagaatac
     9361 aaagaaagtc caaaaatcca aatcgatgga gaccgctata attttgtgga agatcctcaa
     9421 gcaaaagaag aagtcttgaa aatgatcgaa gaaaaattag cagagattcg ccaaaccaaa
     9481 gtagccattt gatttacagt tgctcaaata aagggtaaac ctttatttga gcgacttttt
     9541 tctttatact ggaaacaaat gtcaggagga tagagtatgc gggaaaaaca ctattttaat
     9601 caagaccgta atttttggat caatgtacag caaaacgatg tgctagctgt taaaaactta
     9661 aaagaagaat atggcattag tgacgagatg ctgacttatt ctctagataa aaatgaacgg
     9721 gcgcgcgtag agtacgattc ttttgatgaa gcgttactgc ttgtatcaaa tgttccgcac
     9781 cagcaaaaag tcgataatca ttatgaaact agtcctatcg cttttatttt aaaagaagac
     9841 ggtttgttta catttaccac gccgaatacc gagtacgtga ttcgactaat tcgatcgctt
     9901 ttggaacgaa tgccagacat gagtgtatac agtttattgt ttcgtacatt gtttttgatt
     9961 tctgattcct tttttccttt aatcgaggaa gtgaatagtg aacgtcagcg gcttaatctg
    10021 aaattaagag aaaaaacaac gaacaagaat ctgctgcaac tttcagattt agagatcggt
    10081 ttggtttact tagttacagg aacaaaacaa aatgtagtac ttttagaaca aatcaaggca
    10141 ttggctattt atcgcaagtt gtcagaaaag gaaaaagaag agttggacga tgcgctgatc
    10201 gaagcacgac aagcagttga aatgacgaac ttagcttcgc agattttaga ccagctctct
    10261 ggcacttata ataatttgct gaataacaac ttgaatgaca cgatgaaatt tttgacggtc
    10321 tggtcgctga tcttgaccgt tccaactatt gtgacaggat tcttcggaat gaatctccag
    10381 ttacccttta cccattccgt tttcggatgg ggaatcgcct tgatcatcag tttggtctta
    10441 tctatttgga tgcttattgc actgtggcgg cggattcgtt gatctagtct ttgatctgca
    10501 aaccaagggc ttgggcgatc acaagtgctt gctcttggtt gacgattagt cccgccaaag
    10561 aatcttgaga taaagcgatt gctgtgaaga aattagtcgt aaagtctaat cccttcagag
    10621 aagttcgaaa ccagttcgaa ccggtcaact gattgtcaga caaaaaaagg tttttccaag
    10681 tgacctcata aaattcgctg tcttctaatt gacaatgatc gaaggagaca gctttcaagt
    10741 ttgtactact aaaggaagcc atcgaagcaa tacaatcagt gaaagtacag tctcgaagat
    10801 aactttcggc aaaattcgta cctgttaatt tacattgatg aaacacgact tggtggaaac
    10861 tgccaccaat ccactccaga ttggaaaaat cacatttttc aaaaatgaca ttactgcatt
    10921 caaaatgacg taaaactgtt ttttgcatcg taagtttttg gatatgactc tctcttaata
    10981 ccatattttt ggcatcgaga tagctttggt cacccccgtt gatcagaaga ccagagaaga
    11041 tctcctcatc ttctaaataa aactctccct ttgtaagaag aggaagagaa ggtggaatag
    11101 gttcgatttt tttcatgtgg gttcacctcg tttttcttta gtatagcaaa gaagcgggaa
    11161 tcactgctag aaagtctgct aatctaatca ttttcagaaa aggaagacaa actgtatgaa
    11221 aaacattgac aaaaagatta gtttttgcta tactatgatg gttgagaaat aaaagcagaa
    11281 gcacccgctt ctcgccttag ttgataatgt cactgggcta gatagttttc actggttcta
    11341 ttaggtgaag tacgcgcggg ttctgtaaga gaactcgttt ttttattgcc gtttattcgg
    11401 taagcttttc tttctctcat aatatttgga ggtgaatgac catagcaaag gatatgatgg
    11461 ttaacgacgg cattcgtgca cgagagttac gattgatcgg ttcagatggt gagcaattag
    11521 gagttaagac aaaagcagaa gcattgaata ttgcagaaca agcaaacttg gatcttgtgt
    11581 tagttgcccc tggtgcgaaa ccaccagttg cgcgaatcat ggactacggt aaataccgtt
    11641 tcgaacaaca aaagaaagac cgcgaagcgc gtaaaaaaca aaaagtgatc aatgtaaaag
    11701 aagttcgctt aagtcctacg atcgacttga atgacttcaa tacaaaactt cgtaatgcac
    11761 gtaagttctt ggaaaagggc gataaagtga aagcttctat ccgtttcaaa ggccgtgcca
    11821 ttacccataa agaaattggt caaaaagtct tgaatcgctt agctgaagaa actgcggata
    11881 ttgcaacagt ggaacaaaaa gcgaaaatgg acggacgcag catgttcttg acgctggcac
    11941 cgaaaaacga cagtaagtaa tttgaatgac taactggctg ttggacagcc gatagtcgga
    12001 taacgaaaga ttttgaggag gaaatatagt catgccaaaa caaaaaacac accgcggatc
    12061 agcaaaacgt ttcaaacgta ctggtaaagg cggattgaaa cgcttccgcg cgtttacaag
    12121 tcaccgtttc cacggcaaaa caaaaaaaca acgccgtcaa ttgcgtaaag caagcatggt
    12181 ttctagtggc gacttcaaac gtattcgcca acaactagca aaaatgaaat aagaagtaca
    12241 aataacagaa tctgaagatt tatttttatt aggaggaatt atacatggca cgtgttaaag
    12301 gtggaacagt aactcgtaaa cgtcgtaaaa gagtgctaaa attagcaaaa ggttactacg
    12361 gttcaaaaca cactttattt aaaacagcaa aagaacaagt aatgaattca tacaactacg
    12421 catatcgcga tcgtcgtcaa aagaaacgcg acttccgtaa attgtggatt gctcgtatca
    12481 acgcagcagc tcgtatgaat ggcttgagct actcaaaatt aatgcacggt ttgaaagttg
    12541 ctgaaatcga catcaaccgt aaaatgttag ctgacttggc tgttaacgat gcagcagcat
    12601 ttactgcatt ggctgaccaa gcaaaagaag ctttggctaa ataagctaaa caattataaa
    12661 gaggaaagaa gataacgaaa agttatgttc tttcctcttt ttttatttac aatcctccat
    12721 caaatgaaac aggacaataa cgtataagtc taaaatagca taattaaggt tttttaattg
    12781 aaaactgaac atttatggtc accctttaat gaaagcgcta actacataag ttagaatgag
    12841 tttgactgat atattagttt gcttatcaga aaaacaaaat ggacggagga ataacatgag
    12901 tgatattatt attggtattt tgttagttat ctcattcttt ttcatggtct ggtactgtgt
    12961 aaaaggctac aacttgatgg tcggctttgc catcatggct actgtatgga tgggattagc
    13021 gctagtcgga aacacgtttt caccaaaccc ggcgatggaa gggcaaggag taattgatgt
    13081 gttgacgcat atctatacga cggggccagc tgagtacgcc aaatcgatct tggtaaatgt
    13141 cttctttggt gcctttttcg ggcgtgttct agtagatagc ggaatcgcag ctaccctgat
    13201 tcgaaaggta gttgaacttg gaggggacaa gccaagaatc acgatgagct tactatgtat
    13261 tgtaaccgct gttatattca tgtcgatgac aggaatcgga ccagtgattt ctattgcagt
    13321 tattgtgttg ccaatcttga tgtcattagg tatttcagta ccggttgcac tgttctcatt
    13381 tatgggctcg attatggctg gaatctttgc taatatcgtc aatttcaaac aatatcaaac
    13441 gatttatgct ggtttcaatc cagcggctga aagctatact tataatgact atttccaaat
    13501 tggtatgatt gggatgattg taagcttagt tgtggtacta acggtcgcta atatatcaat
    13561 gaacaaaaag aaacgctatg caatggcagc aaacgtacca gctgaaggtg gcgacgcacc
    13621 aatgatctca tggttagccg tattattacc agttttaggg gtcgttcttt tagatcttcc
    13681 aatcatctta ggttttattc tagcaggtat ctgggcattg ctgtttactg gaaaattgcg
    13741 cggaggttat aaagaaatct gtcgtcagtt tgcgaaatta tttacagatg gcgcagtaga
    13801 tgttgcgcca atggttggtt tcttgatgac cttagcgatg ttcaataatt cagctgcgta
    13861 tgcttctcct tatttttcag ctatttttgg agattggata ccgcaatcac cattagtctt
    13921 ggcaatcgta tttgctatat tgacaccact aggatttttc cgcggtccaa tgaatctagt
    13981 cggttcaggc tcagctattt tagctgtcgt attagctgtt aatccaacga tgtcgccagc
    14041 ctttttgttc ccgttatttg caatcacaac gattgctcct cagcatttag atattacaca
    14101 atcttgggtt gcttggggat taggttatac aaaagtaact tctcgagaat atatgaagaa
    14161 atccatccca actggttgga tcatcggagc gatcttatgc ctgatcacgt tcttattgta
    14221 cgggaacgcc tagtaaacaa aacgtcgata aaaacgatta gatgaatgga ggaaaagaaa
    14281 atgaaacgtt ttgtgcgaat gggaattgat gttggcggta cacatacgaa agcagtcgca
    14341 atcgataatg cgacgcacga aatcatcgga aaatcttcag tgaaaacaac ccacgatgat
    14401 gtccgtggag tagccgcagg tgtcgttcag tcttttcaaa attgtttgct ggaaaataat
    14461 atcagtccag aagatgttgt gtttgttgca cattctacca cacaagcgac taatgcactg
    14521 attgaaggag acgtagcaaa agtcggcgta atcgggatgg caaaaggtgg attagaaggt
    14581 tttttagcaa aacgacaaac tagattgaac gatatcgatt tagggaataa gaaaaaaata
    14641 gagattgtca atgcattctt acctgtcaaa cacttaaatg tagaccgtgt ttcagaaacg
    14701 atttcttctt tagaaagaga aagagcggag gtcttggttt cttctatggc atttggagta
    14761 gataatgggg aaccagaacg agttgtttat gaagcagcat cggtaaaatc gatccctaca
    14821 acgatggctt ctgacatcac aaaattatat ggattgacaa gaagaactcg gacagcagca
    14881 atcaatgcat ctattttacc aaaaatgcta gatacggcaa cctctacaga agattccgta
    14941 cgagaagcag gggtcaatgt ttctttgatg attatgcgcg gagacggcgg agtaatggaa
    15001 atcaatgaaa tgaaaaaacg acctgtttta accatgcttt ccggtccagc agcatcagtc
    15061 atgggctctt tgatgtactt gagagcgtct aatggtgttt attttgaagt aggcggaaca
    15121 acaaccaata tcggggtcat taaaaatgga cgtccagcaa tcgattattc aattgtaggt
    15181 ggccacccaa catatatctc ttcattagat gttcgggttc taggtgtggc aggcggttcg
    15241 atggtacggg ctaatcagtc aggaatcatc gatgtcggtc cacgttctgc acatattgcc
    15301 ggtctagatt atgcagtatt tacggagaca gaaaaaatca aaggtcctaa ggttgaattt
    15361 ttctctccta aagaaggaga ccctgctgat tacgtcaagg tagtgatgga agacggagaa
    15421 gaagtgacta ttacaaatac atgtgcagcc aatgtccttg gattagtgca agaagaacat
    15481 ttttcttatg gaaatgtccc atctgcacga aaagccatcc aagcattagc tgattattgt
    15541 catacgacgg ttgaagacat cgcagaacag atcatggaaa aatcctatgc taagattgaa
    15601 ccggtgattt tagaattggc agacaagtat cacttggaga aagatcagat ttctcttgta
    15661 ggtgtagggg gcggcgctgc ctcattgatt acttacttta gtaataaaat gggtgtgaaa
    15721 tattcgattc ctgaaaacgc cgaagtgatt tcttctattg gtgtagcact tgcaatggtc
    15781 cgagatgttg tagaacgtat catcccttcg ccaagtaaag aagatatacg atcgttgaaa
    15841 aatgaagcga tgaataaagc gattgaatca ggcgctacac cagaatcgat cgaagtccac
    15901 gtagaaatcg atccacagac ttcaaaagta acggctatcg ctactggttc tacggaagtg
    15961 aaagcaaccg atctaaccaa ggagatcaca acagaagaag cactagagct tgctgcagag
    16021 gatatgcgat taaataaaaa tgaagtttgc ttattggaaa atacaccttt cttttatgta
    16081 tgcggcgaac agaatcgttc aaaaaatgct ggaagtcttc ggatcatcga tcaaaaagga
    16141 tttatcaagg tccaacgtgg acatgcttca tgtatgaaaa caactgcagc caattatatg
    16201 acagctgtcg aacaactttg ggaagatatg gcagtttatc aaactgaatt gattgcccga
    16261 ccagaatttt atctctgctt aggagcaaga gtctctgatt ttacagcgac ggatctggaa
    16321 caactacagc ttttgatgga tctggaagta tctactatgg aaccggagga agaagtgatc
    16381 gtcgttgccg gaaatatcaa acaaacataa gttagaagag ttactaaacg aattacaaca
    16441 gatccccgaa gaaatttggg gattctacca gtttcggcga gatttatttt ggaaaaagat
    16501 tcctttgtcc aagcagaaaa ttcttataca acaatcaatc gattgtggta tcgaaacggc
    16561 atgtagcatc aaaaaaaagt acccttttgc cgatgtagga gaaatttgtg aacaaatggc
    16621 tgttccaatc gtttcctgtg aatcagagca aataaacgaa cgaatcactt ttgcaactta
    16681 tgcagaagat gaagggattc gattgatgac ggagccgtta gaaaaattaa aatgttcagg
    16741 actagcttcg atctctaaag aaacagcgca agctttgatt attggacatg aactatttca
    16801 ccacatcgaa gcttctgtta agggaattta tacgcaaagc gaaaaaattg tgctgtggcg
    16861 gttgcctttt tatacacatc aatcaaacat tcgtgcattg agcgagatag cagcgatgag
    16921 tttttcaaaa gagatgaatc aaagcgattt ttcgccttat gtattagagg cagtcttact
    16981 ttggccatat aatgaaattc gtagccaagg gatcttagaa gaaataaaaa aaatagaaaa
    17041 aagatgtacg gagtatgatt tagattacaa ataaagagac tgtgacaaaa gtcgtgtcac
    17101 agtccttttt ttccgaataa acgatgttct cctgtcaact cctcggtcac aagtcaaaat
    17161 attcaacaaa catgagaagc tgttttttga aattgttcct tgtgactcgg agatgaacgg
    17221 caggctcacg acctcttgga aaacggtgtt caaaagcttg catctgcggt aataagtcca
    17281 gccaagttaa aaacataaga agctattttt acttggctgt tcttattact tgatgcaggg
    17341 cagctttttc acaacctctc aataaacggt gtgcaatcag ctgaccctcg gtattagctc
    17401 aaaaaaaacg gcaaaatatg aggaactatt ttgccgtttc tttctctaat actcaggtca
    17461 aaacgctgat tccaaaacct cttatttgat gggtctgtcc tgaatatctt caaaataatc
    17521 ggggtaatta tgaatcacgt attcttgctc ctcgataatc atatgtaaat gcatcgtgca
    17581 aagaaatccg acttcatcaa agttatgatc aatcattgat tgaagcattg tctgatgttc
    17641 atctaatact cgaccccagt ctaattctga aatcgtcaaa cgtaaccatc tgaaacgcgt
    17701 actataactt tctagccaat cccaaatttc tcgttttcca gcaatttcat aacaagttcg
    17761 gtgaaaatca gtatccaaat caaaaaaagc ttttttattt tgagcgatta ctgcctgatc
    17821 ggtttgttct agtatttttt ctagtttagc ttgatcttgc ttggtcattt tcgcactgca
    17881 ttcttgcagg atcggttttt ccaacttttc ccgcgtataa cgggctaatg aagcagatcg
    17941 gatatcgatt tttgaaacat aagtgccgga ctgtggaatg gtttttacaa gctcttgatt
    18001 gcgcagttgg atcagtgctt ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa
    18061 cactttatcg gagattttac ttccaggtgc taactcggaa taaatgattt gtttacgtat
    18121 agattcataa gcttgctgtt gcaaattatt gataaccatt taaccttcct ttctatgcag
    18181 atataatcta ttataacata aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc
    18241 aaaaaaagcc ctgataggca tgcctattag agcctcgaaa agaaaaatat tgaatcaatc
    18301 tggtcgtacc tcttggatcg gtttgacacg accgccgatt cttttagcaa aagcttcgga
    18361 ttcttttttg tctttgaaaa tgagcagatt gtctaagttt ttttcccgag tgcgacagcc
    18421 agttttatcg atgtaaccgt gcattacttt aacgacatac atggggatca cctgcttttt
    18481 tcttttcatt atacgaacta ctcgacacaa tacaagaaaa atgcttagac ggtctttatt
    18541 aagaatttac atcatccaaa gaagcgtacg cttggaataa ctgatctaac tccggattga
    18601 ctttgctcag cttcactaat cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc
    18661 cagtagtttt taattttttg tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg
    18721 tccccgtata cttttggata gttggatgga tgctcacttt ttctccgtaa tggatcattt
    18781 ctttatagct gcaatttact tccagaacgg ggatgatgat cccagattct tcgatttttt
    18841 tatagggaaa ccctagatgt accaataagg ctacccgagc ctcttcaaac cagcggatat
    18901 aattgctgtg atgaataatc cccatttgat ctgtttcata ataaaaaggt tctcgcacat
    18961 aacctgtaaa tttctccatg actcatgcct cgttttccat tgatttttta caaaaataat
    19021 tcatccattt cggaagtgct tcgctgattt gtgtcggcaa aacaatatat tgttcttttg
    19081 ccagctggtc tccaatgaaa ctatgaagat aaatagctgc tgcaatcgtt tggtcatttt
    19141 gaggaaactg ggctaaaaat cctgtgatca tcccagctaa agtgtcaccc atgccgcccg
    19201 ttgccatagc agcagaacct aaaggattgg tgtagaagtg ctggtgacca taaatttttg
    19261 tctcatgact tttcaaaacg agcacagcac cgatttcctg ttgcttcttt ttgttttctt
    19321 tatcccgttg ttcttgtatc ggtagagaac tcaaccgttg ccactccatt tgatgaggtg
    19381 taagtacagt ttgttcggga tagggtagca aaagatgatt agcagcaagt aaacttaaag
    19441 cagagccatc aatcaccagc cattgttctt ttgattggtt ttctagtact tgtttgaaac
    19501 gttccaaact cttagaagaa gtacccaatc cgggaccaat caagagtaca tctgcttgct
    19561 ttattacgga aagaaactgg tcagtatccg tccaaactac aatcattgct tctggcagat
    19621 gactgtgaag agcactccga tttttttcat cagttactac agtcgtgaga cccgctccgc
    19681 tgtgtacggc agctaaagca ctcataatga ttgctccgcc atattgttca ttgccgccaa
    19741 tcaaaacgat acgtccaaat gttcctttat gtgaaatttt tggacgaggt gtgatgactc
    19801 ttttcactag ttgttcgttt atcagcatct aaaaacctcc ttttactcat tatagcgtat
    19861 atttttttta gagaaaaaca gataagaaaa ggaattgtca gattttttga actatttttt
    19921 tctgtaattt gtggtatcat gaacacgaag acttaaataa gtggaggatt aactttatga
    19981 caattgattg gcaaaaagaa gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa
    20041 atcttttacg tgtcaacagc gagcgcgatg actcaaaagt aacaccagat gcaccatttg
    20101 gacctggacc tcgcgacgca ttgaaacata tgttagctta tggcgaacgt gatggattta
    20161 aagtaaaaaa tgtcgataac tatgcagggc atattgatct tggcgaagga gacgaaacgc
    20221 tagggatatt tggacacatg gatgtcgttc ctgcaggaga tggctgggac acagatcctt
    20281 atgaaccagt aatcaaggat ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc
    20341 caagtatggc agcttactat gcgatgaaga tcattaaaga acttgacttg aaactatcaa
    20401 aaaaagttcg cttcgtagtc ggcagcgatg aagaaagcgg ctggggcgat atggcttatt
    20461 atttcgaaca tgaagaagaa ccagattttg gtttctcacc agatgctgaa ttcccaatca
    20521 tcaatggtga aaaaggaaat gtttctttag ctttacgctt caaaggcgac aatgcaggag
    20581 attatgtctt gaaatctttt gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa
    20641 ctgcagcact tcaagtacca agtgcagatg cagctatcgc gatggaagaa gcattttacc
    20701 aattcatcga agcaaatcca gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa
    20761 tcgaattagt tggtaaaggc gcacatggtg caagcccaca atctggtatc aatgcaggtt
    20821 ctttcttggc attgttctta gataactatg aattcctagg atctgctaaa caatttatcc
    20881 atgtagcagc tgcttatgtc catgaagatt tttatggaga aaaactagga gtcgcttatg
    20941 aagatgaaaa aatgggcaaa ttgacaatga atgctggact atttgctttt gaagaaaatg
    21001 gtacagagga agcgaacttc atcaatatga atttccgttt tccaaaaggt gtaacagtag
    21061 acggattgca aagtgacatc gaacaaacag tggggcaaga aggcgcaaca gttactcgag
    21121 gtgctcgcgt aatggaacca cattacgtac caatggatga tccattggtg gctacattat
    21181 tgcaagtcta tgaagaccac actggagaaa aaggctacga acaaatcatc ggtggcggaa
    21241 cttacggacg tttgttgaaa cgtggcgttg cttatggagc gatgttccca ggatatactg
    21301 atacaatgca tcaagcaaac gaatttatga gtcttgatga cttgttcaga gcaacagcaa
    21361 tctatgcaga tgccatctat cgtttggcga aataattaat taaaaagagg atcaagaatt
    21421 gcaaattatt tttgcagtct tgatcctttt tttactttct actgtggtat tcatgataaa
    21481 catgatatag attcaattca tattctttct tagcgttcgt gacgactgta tccaaaatca
    21541 accctgttac taatgaaaga agtgctaaag tcatcaatcc agtacttaaa atcgcagaag
    21601 ggatgcgtgt aataaagctt gtcagcatat attcgcgaat cacaggaatc cccgtgacca
    21661 atccaaataa aaagaagaag atggtccaaa tcccaaagaa aagcaaaggc ttataatctt
    21721 taaacatttt aataatcatc atgatcactt tgaatccgtc agagaatgta ttcaattttg
    21781 attcgctgcc ttcaggacga tcacgataat cgattgggat ttcaacgaac ttgaattttt
    21841 tatctaaagc atggatcgtc aattccgttt cgatttgaaa accagaactc ataataggaa
    21901 aacttttgac aaagatacga ttgaatcctc gatagccggt catgacatca agaatttttg
    21961 ttttatacat tctagtaatt gtatttctaa caaggttatt tccaaaatcg tggaaaggtc
    22021 tcttattctc atcaaagtag gttccatttg ataggcgatc accaatgacc atatctgctt
    22081 caccagaacg taatttctcg agcaaaccat gtacagcttc tgcaggataa gtatcatcgc
    22141 catctaccat taaataatag tcggcatcga tatcaaagaa catttggcgg atcacatttc
    22201 cttttccttg acgaggttct ttttttacga ttgctccacc ttgaacggcc aattcgtatg
    22261 ttcgatcggt cgaattatta tcatagacat aaatatctgc ttctggtaat tctcgtttaa
    22321 agtctgcaat gactgttgaa attgttgctt cctcattgta acaaggaatc agaacagcaa
    22381 ttttttcttg ttgttccatt tttcactctc cttaactata gaaaacaaaa gctaaattaa
    22441 aaatacctca taatcaaagg aaatgctagt ttttcccgct aaatatcatt tatttaaaaa
    22501 gaaaatactg gaatatatgt ttggaaagct ctatctaaaa aaccacccac tacaaaagaa
    22561 ggacatgata cctctctttt gaatagtggg tgcgtattcg ttctctcact tttttgacgt
    22621 attaatctgt tgcacctttt tttcttgaaa tttctaatac ggttcctgta tgagcatctg
    22681 ctaaaaattc ataataaaca gtttcgccat cttctattcg agaaagacct ccccgataag
    22741 cactcgaccg aacggcgaat ttcctaatag gttccttttc aaaagagatc caagaacctt
    22801 cgattgcacc ttcttttaga aaagatgact tgatattttc aagtacttgg tctgctgaca
    22861 atactttttt gcgatgatac caaatcgttg aagctacccc gctcaataat cctagaccga
    22921 ttccaactgc taacccgcct ttaaaatagc ttagttcgct ttcttcatac atgtggacat
    22981 acctccttct tctcacttgt tactccttgt gcttatttta ccacactctc atattttctt
    23041 tgaaataaat aaccgctacc agaaaaaata agctataata aatacgatcg gtctgcagaa
    23101 aaaatcatat tttatccgca gatcaatata agtctgagag gagtttagtc tgaaatttag
    23161 acgaatagca tatggaagaa aaaacatttc aacgaatcaa agagttaaca gaattacaag
    23221 gaacaagcgg ttttgaacaa gatgttcgcg cttatatgga aagtcatatg gaaccgctgg
    23281 tagatgagtt acagttagat ggtttgggcg gcatttttgg tctgcgccat catgaagaag
    23341 cggatgcacc ccgtgtaatg gtcgcagcac atatggatga agtcggattc atgctgacac
    23401 aaatccaaga aaacgggctt tttaaagtcg tacctctagg cggctggaat ccttacgttg
    23461 tttctgctca gcgttttaca cttaaaacct ctactggtaa gaattatcca tgtatttctt
    23521 cttccgttcc cccacattta ttgcgaggaa caagcggaca aaaatcagta gaagtaaccg
    23581 atattttgtt cgatgctggg tttgaatcaa gagaagaggc aatgagttac ggtgtccttc
    23641 caggagacac aatcgttcct tacgctgaaa cgatcaaaac agccaacgga aagaatatta
    23701 tcagtaaatc atgggacaac cgctatggct gtacaatggt attagaagct ttagaagcgt
    23761 tgcaaaatga aacattaggt catacattga ttgcaggcgc gaatgtccag gaagaagtag
    23821 gcttgcgtgg ttctaaagct tctgtcaata aattcaagcc agatctgttc tttgcagtag
    23881 actgctctgc agcagatgat acagtgacga aaaatggcac attcggacat ttaggagaag
    23941 gtacattgat gcgtatccaa gatccaggtt tgatcatgct tcctcgtttg cgcgagtact
    24001 tgctggatat cgctgaaaca aacaatatcc cttatcaata ctttgtttca aaaggcggta
    24061 cagatgcagg tgctgctcat acacaaaacg aaggaatccc aagtacagtg attggcgtag
    24121 tgggacgtta tatccatacg catcaaacga tgttcagtat tcgcgatttt gaagctgcac
    24181 gagaaatgtt gattcaaaca ctgaaaggtt tggataaatc aacagtaaat acgatcgtat
    24241 acggaaaata atgcgatagt gaaactataa aatctggcaa aagattgctt tttgccagat
    24301 tttttttata aaaagaggat caatgtttgt tagttcgaaa gaaaggtgta taatttaatt
    24361 gaaaaataca taaacagaaa gtagaggaca aagaatgatt atcccaaata gtttagaaga
    24421 attagcaggc tacgtagaaa ctggaaagag tgtatttttc tttactgccg gctggtgcgg
    24481 agactgccgt tttatcaaac cgcaaatgcc tgaaattgaa aatgactttt ctgattggca
    24541 atttattgaa gtagaccgtg acaagtacat agatgtagcc gctgaatgga acatttttgg
    24601 tatcccaagt tttgtcgtga tccaagacgg aaaagaatta ggtcgtctag tgaataaaga
    24661 tcgtaaaaca aaacaggaaa tcgaatcgtt cctagaaagt gtaacaaaag gctgacatct
    24721 gttaggaggg gtcaagatga atcaagaata taagaatatt ttagtaggta tcgacggtag
    24781 tgaacaagca cttgctgctt tcaaaaaagc ggtagaagtg gctcgccgta ataatgggaa
    24841 agtatatgta gcaaacgtaa tcgatcagca attttataac tttatgggct atgcacctat
    24901 tgatcaaaat ctagtcgatc agcaaactga aacagccaag gaaatgatcg aagaatgtaa
    24961 agaatatggg aaatctgttg attacacaaa tatagaagga attattgcat atggttctgt
    25021 tcgtgaatcc atggcagtca aattgccaaa aaaatatgag atcgatctaa tcatggttgg
    25081 gcagtcaggt ctaaatgcag tcgaacgctt tatgacagga agtgtagcca gctacgtgat
    25141 acgtcattca ccttgtgatg tcttgatcgt ttctgacgaa gaagaacagc caagtaatta
    25201 agcaagtgga acttgtacga cctttttaat caattaacta aaacagtaaa gggactgtga
    25261 caaaagtctt gtcacagtcc cttgttacga ataaatggtg ttccagaaga ggcttcttcg
    25321 gaaataaagc atgaaaaaaa tttcatgcag tgaatgcaat tttttgttaa gataaatctc
    25381 agcaaaggat aatggaggaa agaacatgat ttttgcttat aacaaagcac acgtcggaga
    25441 caccttgatg gttattatag ccgacgataa aggaacagaa aacactgttg aacgcaaatg
    25501 gaatgttgct cggatcaaag atgaacaagg tcaagttgtt gcttggaact ttttccatat
    25561 ctccgatcat ctaacaatcg aagggaacgg ccaagtaagc ttaacagatg aggaattagc
    25621 gaaattgaat cgattgatca aagaagcagg attcgaagaa cagcttgaag ctgatcatga
    25681 accgaagatc gttgtcgggt acgtaaaaac atgcgtccca catcctgatt ctgaccattt
    25741 atcgattact gagacagaag tagacaatgg tacagtattg caaatcgttt gcggtgcacc
    25801 caatatcgaa gctggacaaa aagttgtcgt tgcaaaacca ggagctatga tgccagatgg
    25861 aatgatgatc tggccaggaa ctttgcgagg ggtagatagc tatggaatga tctgttccgc
    25921 tcgtgaattg catttgccaa atgctccagc aaaaaaagga atcttagtat tgcctgagga
    25981 tgccgtagtc ggagaagcat ttccaaaata aagaggttgt gacaagtctt tgtcacaacc
    26041 tcttttttat taattttcac tattttgatt ttgttttaat gccgattgat cgatcgtcaa
    26101 ttcaacagta gttgtttttt ctttcgagcc ttcatagtaa gtgattttga cagaatctcc
    26161 cacttttttc ttatacaaag cagattgaag ttctacacca gaagaaactt ctttgtcatc
    26221 gatcttagta atgacgtcgt attgtttcaa tccagctttt tcagccggtg tagctgtttg
    26281 gacatttgtc acgatgacac cgttagttac agatgaagga atcttcaaga tttgttcttg
    26341 ttgctgagta gaaacggcag ataagtcgac cattgtgata cctagagctg gacgtgtcac
    26401 ttttccgtct ttttccagtt gattgatgat attcactaca tcgttacttg gaatagcaaa
    26461 tcccattcct tctacgctta cattagaagt agattcagaa gtacttgcga tcttacttga
    26521 gttgataccg ataacttgtc cttcgatatt aactaaagga ccaccagaat ttccagggtt
    26581 gattgctgca tctgtttgga tcgcattgat attgactgtt tcgttcgatt cattcgtact
    26641 tgtcacttgt cggttcaagg aagaaatgat tcctgaagtg acagaatttg catattcaga
    26701 tcctagtggg gaaccaatcg caatcgcagg ctcaccaacc ttcagagcac ttgagtcacc
    26761 aaaagaggca actgtttcga ctttgtctga attgatcttc aacaccgcta aatctgaata
    26821 agcatctgtt ccaactaatt cagcttttac cttcgtaccg tctttcatca agacttcaag
    26881 accttgctgg ccatctacta cgtgattatt cgtgactaca taagcagtat tcccgtcttt
    26941 tttgtagatc acgccgcttc cttcactata agcttccaaa ttactatcat ctgagctgct
    27001 actttcttct tgctgcccga atagtccgcc aaaaccgctt gattggtttt gactttgtaa
    27061 gttgataaca gaaacgacag cgtcttgaac tttatctacc gctttggtga tatcagaatc
    27121 tacattgact ttgacatttt ccacaactgt ttcaccggca gaattctgat tgccgcttgt
    27181 agcagtagaa ctattgttgc cagtgcccat cgctgcataa aaaatgccag ctgtcagtag
    27241 tcctccaacg atccctccaa caagccccag acctaactta cgccagaggc tattattttt
    27301 actttttttc atctttggtg tcacattttt tctatccatg tgttcctcct ccaattacat
    27361 tcatttatct actgttagta tagtcttatt tgttgacttt agtctacttt gagactgtga
    27421 actttttatg aaaaacttta ggaaaaatca ttgttttcag atgaaaatat ttgattttct
    27481 ttttccacag atactgtgta aaactacgcg gataggattg gaaaaataag ggataaaaca
    27541 tatccacagc ctgtgtataa agtggataaa ttcttataaa acgtatgttt gcctaatgaa
    27601 tgtgatgaaa taaggtaaaa taacactgtt ttctgtggat aaacctgtgg atgttgtgca
    27661 taactgttgg ggaaatcttt ttaacaaaaa tagccaaaat aaacatatag acgaaaaaat
    27721 tgattataat aaacaaagtg agcatgaaga ccaaaggaga gaaaacatga atatcaaaat
    27781 tatctcagta ggaaaattaa aagaaaaata cctgatccaa ggtatcaatg agtatgtcaa
    27841 acggctgaat gcctatgcaa aaatcgaatt gatcgaagtt cctgacgaaa aagccccgga
    27901 gaatttaagc gaagcgcaaa tgagacaagt aaaagaaaaa gaaggggaac ggatcttagc
    27961 gaaaatcaaa gaacaagaat acgtctatgc cttagcaatt gaagggaaaa atcctacaag
    28021 tgaatctttt gctaaacaaa tcgaccaatt aggtatccaa ggaaaaagcc accttgtttt
    28081 tgttatcggc ggctctttag gactcagcga agccgtcatg aaaagaagca atgctcagat
    28141 ctcttttgga aaaatgacgt atccacatca attgatgcgt ctgatccttg tcgagcaaat
    28201 ctaccgtgcc ttccggatca atgcgggggc tccataccac aaataatgat attttttaag
    28261 agaaaaaatt atccctattt taaataatag caataggtag tggtagggag aaatttataa
    28321 ataacttttt tatttgcttt ggtagccaag taagttgcaa aagaaaaata gatactgtta
    28381 aaaagataat gtagacatag aaacaaatac gtaaaatttt agttagaata atggcaagga
    28441 gaaaaaacat gatagatgaa aaaaagtgtt taaaaaatct attagaggat atggatgata
    28501 ttgaagaaaa ctatctaaat ggactgggaa aacaaatata tttcagatac ttaagctatc
    28561 taaaaatgaa ataagacata gtaatttttt agcttttttg tttgctccaa atgaaaccca
    28621 caatttatct gatgagtttt ttaaaatgtt tttgaaaagg tatatagatt ctaatgatga
    28681 taccaaagct gctatcaatt attttgatgc tttacttaat agctatgaag atttaattgt
    28741 ttacagagaa aataagaata ttgatatttt attggtatcg gaaaaaaata aaatagtggt
    28801 gtgcatcgaa aataaaattc tgtcttcgga gtctggaggg caacttaata aataccaaat
    28861 atatatagaa cgcaattatt ctaactataa aaaaattttt gtttttttaa ctccagatgg
    28921 aaatgaaccc acaaatccta gttggggcat aattacttac aagaatatcg ttga
//
LOCUS       DACCXM010000021        28241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCXM010000021 DACCXM010000000
VERSION     DACCXM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.022"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(241..621)
                     /locus_tag="IVY61_000814"
     CDS             complement(241..621)
                     /locus_tag="IVY61_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7969948.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(625..840)
                     /locus_tag="IVY61_000815"
     CDS             complement(625..840)
                     /locus_tag="IVY61_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969949.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(843..1250)
                     /locus_tag="IVY61_000816"
     CDS             complement(843..1250)
                     /locus_tag="IVY61_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP7969950.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(1516..2991)
                     /locus_tag="IVY61_000817"
     CDS             complement(1516..2991)
                     /locus_tag="IVY61_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP7969951.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(2981..3829)
                     /locus_tag="IVY61_000818"
     CDS             complement(2981..3829)
                     /locus_tag="IVY61_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP7969952.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(3866..4228)
                     /locus_tag="IVY61_000819"
     CDS             complement(3866..4228)
                     /locus_tag="IVY61_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969953.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(4528..5152)
                     /locus_tag="IVY61_000820"
                     /pseudo
     CDS             complement(4528..5152)
                     /locus_tag="IVY61_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(5149..5460)
                     /locus_tag="IVY61_000821"
     CDS             complement(5149..5460)
                     /locus_tag="IVY61_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969954.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(5504..5797)
                     /locus_tag="IVY61_000822"
     CDS             complement(5504..5797)
                     /locus_tag="IVY61_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969955.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            5991..6638
                     /locus_tag="IVY61_000823"
     CDS             5991..6638
                     /locus_tag="IVY61_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7969956.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            6699..7844
                     /locus_tag="IVY61_000824"
     CDS             6699..7844
                     /locus_tag="IVY61_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7969957.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(7925..8230)
                     /locus_tag="IVY61_000825"
     CDS             complement(7925..8230)
                     /locus_tag="IVY61_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP7969958.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(8255..8686)
                     /gene="ruvX"
                     /locus_tag="IVY61_000826"
     CDS             complement(8255..8686)
                     /gene="ruvX"
                     /locus_tag="IVY61_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP7969959.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(8683..8949)
                     /locus_tag="IVY61_000827"
     CDS             complement(8683..8949)
                     /locus_tag="IVY61_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP7969960.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(9204..9929)
                     /locus_tag="IVY61_000828"
     CDS             complement(9204..9929)
                     /locus_tag="IVY61_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP7969961.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(10290..10360)
                     /locus_tag="IVY61_000829"
     tRNA            complement(10290..10360)
                     /locus_tag="IVY61_000829"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10326..10328),aa:Gly,seq:ccc)
     gene            10477..11229
                     /locus_tag="IVY61_000830"
     CDS             10477..11229
                     /locus_tag="IVY61_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP7969962.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            11245..11724
                     /locus_tag="IVY61_000831"
     CDS             11245..11724
                     /locus_tag="IVY61_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP7969963.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(11975..13555)
                     /locus_tag="IVY61_000832"
     CDS             complement(11975..13555)
                     /locus_tag="IVY61_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP7969964.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(13548..14291)
                     /locus_tag="IVY61_000833"
     CDS             complement(13548..14291)
                     /locus_tag="IVY61_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969965.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(14467..15243)
                     /locus_tag="IVY61_000834"
     CDS             complement(14467..15243)
                     /locus_tag="IVY61_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7969966.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(15491..16327)
                     /locus_tag="IVY61_000835"
     CDS             complement(15491..16327)
                     /locus_tag="IVY61_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP7969967.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(16338..17267)
                     /locus_tag="IVY61_000836"
     CDS             complement(16338..17267)
                     /locus_tag="IVY61_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP7969968.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            17459..17812
                     /locus_tag="IVY61_000837"
     CDS             17459..17812
                     /locus_tag="IVY61_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7969969.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(17886..18542)
                     /locus_tag="IVY61_000838"
     CDS             complement(17886..18542)
                     /locus_tag="IVY61_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7969970.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            18768..20702
                     /locus_tag="IVY61_000839"
     CDS             18768..20702
                     /locus_tag="IVY61_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7969971.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(20788..22011)
                     /locus_tag="IVY61_000840"
     CDS             complement(20788..22011)
                     /locus_tag="IVY61_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP7969972.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(22005..23903)
                     /locus_tag="IVY61_000841"
     CDS             complement(22005..23903)
                     /locus_tag="IVY61_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP7969973.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(23897..25015)
                     /locus_tag="IVY61_000842"
     CDS             complement(23897..25015)
                     /locus_tag="IVY61_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7969974.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(25098..25931)
                     /locus_tag="IVY61_000843"
     CDS             complement(25098..25931)
                     /locus_tag="IVY61_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7969975.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(25924..26841)
                     /locus_tag="IVY61_000844"
     CDS             complement(25924..26841)
                     /locus_tag="IVY61_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7969976.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(27068..28192)
                     /locus_tag="IVY61_000845"
     CDS             complement(27068..28192)
                     /locus_tag="IVY61_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7969977.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         8626 a         5647 c         4632 g         9336 t
ORIGIN      
        1 tgcatttgaa tggtcacttg accaaaatga ggtaactcaa tggtgcgctg tttccctgtt
       61 ttatcagtaa taacaattgt atttttcaat aaatctccta ctttcattat atactttttc
      121 acttgtgatt acaagtgtaa tcgttatttg taattaccct tatgcctttt cacaacggtt
      181 atttgtacca aaatgagtag gcatcgattg tcagtaagtt tcacactatg cccccttaac
      241 tcaaagggta ctcttttctc gtctttgtct ttcattggct gtcttggaat tatggtgtgc
      301 atggcataaa gaacgaaaat tagtttcatc taaccgtcta ttccaatcat cttttagttc
      361 aattttgtga tctacaacat cagctttcct tatcaagcct tcttctaggc aatcctcaca
      421 tactggatga tttaaccgat acaagtatga agcctttcgc catgttctac tttgatagaa
      481 ttgaaaatat ttaccttcgc tttgctttct ttcttcacgc ttaattgccg tttgctttgc
      541 tttatgcttc ccacaatact tctgcctata atcaatcaat gttttacatc cagcatgatt
      601 acattgtttt ttaactgcca tagattacca accaaagcga tctcgttcgt cagaatcatc
      661 ttgttcccat tttgatttgt attcaatata aggcgaaact atcttcaagc acgttccgtc
      721 tttaaaatta actttagtga ttttatcttc ctcaaaacta ataaagtcta catcttcttg
      781 agtgtttgaa tactcaatgt gtttctcacc atcgaagtat ctaatacttt ccatatgcat
      841 aattattcta cctccaataa attagttatc aattcatcat ctgttagtag caaagccaag
      901 aacttatcat ggatgtttaa aaacaaatta tcctcattcg atgtaagctc tcgttgtctc
      961 gctcgatcat ataggttaag cagacaatta aattcatcca ttagctcacc tgtatcatca
     1021 tagttgccac tatctaccaa tgatcccact actagcgatt gacaccacgc tactgactcc
     1081 tgcagccaat caggttcttc ttccattatc tttaatactt ctggatcaaa tgtggaataa
     1141 tcttctgtta ccaatcccac actaaatgcc agcgtgttca ttagaaaccg atccatttca
     1201 tctgtatatt gaaagttcgg tgctttgttt gttgaaataa tattgagcat ttactttcct
     1261 ccttgattcg taacacgtgg taacacctac gtaacaggta cccgttacca ctcaatccca
     1321 acggggacaa gggttctagc gttttggtaa caggtaacgg gtaaaatcca gtaacattat
     1381 tccccagcat attagctaac tatttcaaaa gttttaagga aataaggtgt taccccgtta
     1441 ccaaattgct gtatcccttg gggcagtaag ggtaaactag taacgggcta ggtgttactg
     1501 acccgttact acccgttact ttttgtactt tccagctcga tttttatttt cataggcatt
     1561 tgacttattg taaaatcctc gtcgtttatt tctttttggc agttgcttcc gttcccaacc
     1621 aggaatactg ctaataatca aggcaatctt tttagtttct gcatctgatc gaccattcaa
     1681 taaatccttt gcttgtttat caaaaacagc ttccaaaata tcagcggtga gtacgctatc
     1741 aactaaataa aactcttgtt gcatagtagc tccattatag aaatgctgta aatttttctc
     1801 attacattta ctgtcaggat agttctgata ataagaacgt ctcgcccacg ctggagcttc
     1861 ttcccattta tacgggattt ccatttccaa atatttagtg attgcttctt taattggatt
     1921 ctccatcttg gcatcctctt gatagtcttt tgcaatttct aagacttctt tatcttcatc
     1981 gggactaaaa tagattcgtt gtcctttatc aaaaagcacc tttgcttcag ctaacacttg
     2041 aagaaaatat tcatcctttg ttttaaacgg attcatcttt ggcttattct tacaaggcaa
     2101 aggataaaaa cggcggtttc ctgtatcatc tttcaaatac tgcccatcat tcgacgttcc
     2161 aataaataca cattgccttg cccaagctat gacattccga ccataaggag gtctaatttt
     2221 atcgatcttg gcactgatga agttttttat ctgttcaatt ttagttttgt tcattgaaga
     2281 taattctcca agttcaataa ttacattgcc ttgaagctgc atataatcat ctttcttttc
     2341 acctagcgaa tctaatgaat cggtgaaaaa atcatccgtg taaagtaatg ctggaacggt
     2401 actcttacca atcccttgcg gtccatccaa aactggaacg atctcaaact ttattcctgg
     2461 tttatagata cgtgcgactg atccgactat ccaacgtttt gttacttctc gtgtgtaaag
     2521 gttgtcgtct acccctaaaa ggtcaataaa cagtgtttcc agtctaggct gttgatccca
     2581 tttaactttt tcgatacgtt cttttactgg gttgaaaaca ttctttctag ccacatttac
     2641 tactgctttg tgtattttgt tgtcatcaaa aactacattg tagttttctt caatatatgc
     2701 cgttaaggca tcctcaaaac tgtcttccca ttctccagcg gtacgattga tccaaggaga
     2761 atcttttaat aggtgaatgt atccgctaaa ttcgttataa gcaattgttc cagctaaatt
     2821 gctatcattg gttaaaatca attctgcatt cacaacactg ttacttttaa tctgatcgtc
     2881 tttattgcat cttaatcgtg acatccaggt tttcaattca atgatattgt cttcacgctc
     2941 gctggcttcg ggaaagtcta aaatactatc taatttatgc ttatgagcca tgagctttcc
     3001 ccctcaaatc ttttttaatc atcgatcgaa taatttgatt cacttcttta tcttttagcg
     3061 gtggttcatt gtacgcattt gctgtataaa tcaattctgc taaagtttga ttatctacac
     3121 cgtgccatag taaccaaccg cataacttag ttaaatgatc gtttctacta ccttcgccag
     3181 cgccttgaac aatcatatct aatttttttg ctgtccctgt ttttgctacg ggtcggtatt
     3241 gagtctttat ttcttgcggt ttttttagca gtaggttcac tagccaatcg ggagctggtg
     3301 caatattatt tagagatacg ttttcaatag gttcatatgc tctcccaaca cttcttgaaa
     3361 taatcatagt tggcgctatt ggaactcctt tagtgataat atcaatcccg cttaaacttt
     3421 tacctttatc atcacgcccg aagatatcag caaatgcacc tgtttcttgt tggataatta
     3481 caccatcagg aattttaaag aagaaatgga ttcctttacc tggtgtgatt tccgtataag
     3541 tagaaggaaa tggatcatac attgaagcaa gttttttata attctcaacg ccgtttcgtg
     3601 tttcagaatg ttgatctagg tcaactacca ccatttgatg atcttcaagc attaacccaa
     3661 tatttctagt tgcatcttcc gtccaccatt cattaatttg attagaatcg ttagttgcat
     3721 cattccaacc actagagcct tttattggaa ttttagttcc aggagataag ggataaattg
     3781 accatcccga cttttgtaat tccgttgctg cttgataatt atttgccaat aaacctctcc
     3841 tcctttttca aaatactggt ataaatcatt cagacacctc catgagcttc aactgctcaa
     3901 gttcgtattt caacccatcc cgaacaaatt cagcaccttt taattgattc tgatacttta
     3961 cccatgtatt aaatgttttc acccttgctt cttgcatatc agtaatgttt aaccatgcgg
     4021 ttaaaatctg attttttagc tcgttgattt ctcgacaaat tattttgtgt tctgcatgtt
     4081 ttttgagtac atacaaaact gttagttttt cattgaatga atttccaaag taggttttcc
     4141 cgatattatt ttctagttca gcgatccctt gattaatttc ctctaattca aggctatcga
     4201 gttggtttaa tagttttgtt ttgtacatct acttatgccc tccagttctt aattgatagt
     4261 cgtactcgtc ataagcagtg aagagatata acgctaatcc tgcaaagata gctatttttg
     4321 caattaatgg taataagccg aaaattccgc cgattaaaat gcttcctata attaagtttc
     4381 gtttcataat ggttccctcc gattttttaa tatggtataa tcggaacaaa gaatatcggg
     4441 ttttatccca attaagttgc ctttatcctg tccgccaaga ctgttatgga taaggctttt
     4501 tctttgttct cgatcatgtt aatgctccta agttgtctgc aattcagcag aacgattata
     4561 aattttctca ctcactgaaa actgcaaccc atatttttct ttaacagtca ttaattctac
     4621 tgtctcatct aaaataggtt cacgatcaac caacatttca ggagtcatat ctttcttttt
     4681 taccattttg gaatgaccat aaatagttga tacagcttta ttggctatca cattcgcttt
     4741 aatgtagtct actggttttg gttcagtaat agcgtggctt aatttagtca ttgcttcttt
     4801 ttggtgttct ttgtctagca tgcggaatac ttgaaagcct tcaagtcctg tggactggcg
     4861 tagttctttg atgatgtcga aaatccagtc ttgaaatgcc tcggcttctt ttttacggct
     4921 tttgaatgcc atgcggtaaa tatttttttt cgttgatgat gtttacatct tgtgttctgc
     4981 ccaatgaatc gatgacctta ctagtagtaa ccccatcttt aggcaaagag tgaatggctc
     5041 tagtaacctg tttaagacct aacgcttccg caacatcttt cgccactgcc caccattcat
     5101 cattaatatc gacaaatctt ataatgtgtc cgttccaaat ttcagttttc attcaacgtc
     5161 ctccagttca agccctagca agctcgctaa caactcaact tgctgataca aaatttcttg
     5221 atactcttca atagaaagtc gttctttaaa ctctgcttgt actaaatcgt tcaagccacg
     5281 caatactccg cttaccgttg gttcttgtgg tagcaatttt tgatagtcca tttgttcgta
     5341 caaacattca aacgtttctt ctagcgcttt gccttccaag tttgctaatt ctgccgctgt
     5401 gtcttctgta agaattgttg ataatacttt ttcgattgct ttattttgtg tcgattccat
     5461 tattatttcc tcctattaat ccctttgccc ccacggcttg cgtttatttt tggtattctt
     5521 ccataaagcg atcacattca tcttgtgaaa ttcttacgac tccatcaact ataatcaact
     5581 ttaaaccgtt aggaacatat ttctgcatca tagtattgta tgaaacgttc atatacttag
     5641 cagcttgtgc cttgttcata tattttggaa actggtactt tttttctaat aatgaaagta
     5701 acgttccttg agtttgctct acttgttgaa caactcgatc gtcaataatt ttaaaagctg
     5761 gatcatcaag cgtgagtttg tcaattttta attccatatt tatcccccct cgttagcata
     5821 catttctata caaagatttt aaaaaaatgc aagaggctct tgaatacatt tctatacctc
     5881 ttataaactc aatataccat catacctttt catttgcaag aattttaaca tacttttttg
     5941 aacttttatg tttcacacat atttaaatcg tgatatagta gagaaaatat atgaatggtg
     6001 gtgaaaatgt ggctagaaaa aaataccccg aagcagtgga gacaggacaa agaattagac
     6061 tggctagaat tgaaaaggga ctctcaatgg aacaacttgg aggtaaatta tccccaccgg
     6121 ccagtaaagg tgccgttagt aattgggaaa atggctataa tcttcccaat aacaccaggc
     6181 ttaaacaact ttcaaaggta ttagatgtat ccactaccta tctattaaac ggatcttata
     6241 cattaagaga tatgcatatg atgcccgaaa ttaagcgaaa tgtgcttgaa aagcaattga
     6301 aaaaaactat tgaaaaaaat aactctataa ttgattctaa tctttctaat gctatcaata
     6361 acatcgaaga agataacctg actgtctcac agaagattta tttaacaaac gcaataaaat
     6421 atcttaaaac aggatctcga attgaatttc ctgacgggag taccccaatt gcttatctcg
     6481 cagctcttct aaatacgctt ttacgatatg actcattaat taatgattca gaaaaaaata
     6541 cagatgagaa aaaagagtat caagaatttt atgaaaatga tcttctaaaa gagttcacag
     6601 ctcttgttac tgagctaaat aaacactatt caaaataata ataaactgcc tcctccaacc
     6661 gcccccacgg cttgctacac tatggaggga aaactattat ggcaacattc aagcaatatg
     6721 aaactaaaaa aggtaaattt tggctctatg aagcctatct tggaatgaac aaaatgactg
     6781 gcaaacctga taaggttcgt agacgaggtt acaaaacaaa aaaagaagcc cagcttgcct
     6841 tgtcacgctt gcaagtggac tacgataaaa acggtattaa aaaagcgaac aacgaaacgt
     6901 ttaaagaagt ttatgacttg tggctagaaa catataaaac cactgtccga gaagccacct
     6961 tcatgaaaac tgaaatcaag tttagaaagt ggattcttcc aaaatatgga aatcttcgtg
     7021 tgaatgaagt cacggtaaaa aaagcccaac aaattgtaaa tagctgggct gaaactactg
     7081 accaatacaa ggtacttcat tcaacttcta agcgtatttt tagctacgct atcaatcttg
     7141 ggattattga gactaatcca ctagacaacg tattaatgcc taaaagaacc accactaagc
     7201 ataaagaaaa ggtcaaagtt tacagtaaag aacaactgat caccttgttt gattacctaa
     7261 acagcaaacc tgaaacttat cgaaacgact ttgataaaac gttactacgc tttctctttt
     7321 atagcggggt taggatcagt gaagcccttg cattgaattg gtcagacatt gattttcaag
     7381 aaaatacggt caccatcaat aaaacactaa gccaatccaa aaatggctat aaaatcagtg
     7441 atcctaaaac tgattgtagt gctggggttc ttccactaga tgaaagaaca atttctatat
     7501 taaaaaaatg gcagattaat cagcggaaat atatgctgac acttggtgta actgatccga
     7561 ctatgatttt ttgtggtatc tataaagaac taatcacaca ccacgctatt tatgcaagat
     7621 tgattactat ttgtgaaaaa gctatggttc catttctcgg tgtgcatgta gcaagacaca
     7681 ctcatgcttc aatgctcttg gacgctggag ctactatggt agaagttcaa actagattac
     7741 ggcatacaaa aattagtcag acgatcgatt gttacggtca cttggcaaaa gaaacgaaag
     7801 aaaagactgt tgaaaaatta gtacaacact taaatattga ctagatggca tacaatatgg
     7861 catacagtaa aaaataaaaa aaggagaaac cctatcatat caaggtttct cctagcttta
     7921 tagtctattc ttcttcttcc gccatgaatg tattgaatac ttcttcaatc atgtcccatt
     7981 ctgcgtctgt ttcgatttgt tctaactcgc cttctgttcc atcttcgttt tcgatgtatg
     8041 cataagcttg taattctaca tcttcatctt ctgaaacacc agcaggatat aaaagaacat
     8101 agttacgtcc aaattcttct tgtccgtcaa ctgttagtag gatttcatat aatgtttcat
     8161 ttccttggtc atccaccaat gtgatgtgtt cgtgtccttc atggtcgtgg gaatggttat
     8221 gttctgtcat aagattacct cttttctttt attattaaaa taattgctta cctgagccgt
     8281 ctaaataatt ttgtaagatc atcacagctg ctaatttatc gatgactttt tttcgtttgg
     8341 ctcttgaagt atttgcctgc tctactagca tacgttctgc ctgaacagtg gttaatcgtt
     8401 catcttgata atcaactggc aaggagaata gttctttgat cttttctccg tatgacatgg
     8461 aagcttctgc ccttggtccg atggaattgt tcatattttt aggtaatcca actacaaacc
     8521 ggtctacttc atattgtgct actaattcct tcaaccgatc aaagccaaac tcttcttctg
     8581 cttcattgat ccgaatgatc tctactcctt gagctgtcca gccaaagggg tcactgatag
     8641 caacacctac tgtcttagaa ccgacatcca agcccattac tctcataaat tcactccatg
     8701 atttgagagg tagtccttga caattacttc catgatctca tcgcgttcgt gacggcgaat
     8761 caaatttcga gcatcacgat aacgaggaat gtaggacggg tctccagaaa gcaagtagcc
     8821 gacaatctga ttgattggat tgtagccttt ttcttccaaa gcccgataaa caatcgctaa
     8881 tgtttcgctc acttcttttt tgcgactatc gtcaaaacca aaacgtacgg tttcatctgt
     8941 aaaacccatg attctacacc tctttcccgc aaggtaattc cagcttacct tctacaacct
     9001 tattttactc gaagaaaggg aaaactacaa acaaatattc caaacttccg aataaattaa
     9061 gaaacactat ttgtatgttt ttttcctttt ttctgtagct ttatcaaaag tactcttttc
     9121 ataaagtata cgctatattt ctttgaatgt atgtttttac acaatttttt cataatattc
     9181 ataaaaaatt cgaatagctg ctactaattt tttgtttttg ttgcttgtcc tgtctcataa
     9241 ttgatcgtgt agccatcttg aacattgtag ataaagacat tgaattcaat ttttttgtct
     9301 tcaatgcttt ctgcttccaa ttgcactccc ctggctacca gttcattccc ctcaaaatga
     9361 ggagtgacac ggtagcgtac atgatgattt gtttctttga tatatgccgc aatatcattt
     9421 tcgtgagcaa gcatgtatgg agcattcaac gaacgtgtcc ctgtcatcaa gtttttaatg
     9481 ttgttatttt gaccagtcag ctggaaacca attaaatggc tgcgattata taaccattgc
     9541 ccgtcactta atttcttttg tttccaacct gtcgggtcaa tatacaaagg ctctctcttt
     9601 tcagtaggga acaaatcttt tccaagcata gcattggctt gtcctactct gttcaattta
     9661 tcaatatttg aatagctttc ccatgttcct ttggaaaggc taagatcctc ttttgtaaat
     9721 tccggctgat tcccattgat ttctatttct tgatgttttc catcatattc tggcacatcc
     9781 gctacttgag aagcttgtgg tgtggaatca aaaatagttg taatctgctg aaagatatct
     9841 gaatcttttt gtgtggtgga aacaccgaat gcccctgcaa tcacagcagc gacgatcccc
     9901 acgatcgcct tggttgtttt tttgctcatt tctttactcc tttactaaca gctccctaaa
     9961 tcttaacata tgttccccta taaataaata ctagaaagaa atagaaacag caaaaaataa
    10021 aacctgtttt aagatggctt aaaaatacaa aaaagagacg gattcctcgt ctctaatcag
    10081 tcaccactct atctgtaagg tatttattat tattcaaaaa ttttcttcat actcaagaca
    10141 atacactgat ttcacaaccg cttggtaaac ggtgcgcaaa agcttgcatc tgcggtaata
    10201 agtccatcca agttaaaaac ataagaagtt gtttttgctt ggctgttctt attacttgtt
    10261 gcagagcagc tttttcacaa cctattttaa gcgggtgacg ggaatcgaac ccgcgacatc
    10321 agcttgggaa gctgggattt taccactaaa ctacacccgc aatacaaaaa cagtatacgc
    10381 ctcttttatt ccttcctcaa gtttttttat tctttacgct tctggtatgt tttgtagtaa
    10441 aataaaacta atcaatcaat tattggagaa gatgctatga cgggaaaaga attagatctg
    10501 ttgctggaca cttgtttact cgctgggaaa atcatgatgg aaagcaatgc ggagatgtat
    10561 cgggtagaag atacgatgag tcgaattgct ttagcttctg gaaatttccg tttggtcagc
    10621 tatgtcactc aaaccggtct ctttattggt cttgatcgta cctcgaccat ccgaatggaa
    10681 caaattacca accgttcgat taatttagaa aaagtagtaa aagtaaatga tctttcacgt
    10741 aaatatgttg cttcagagct aacattagaa gaactttact gcgaactgca agcaatcgac
    10801 cgagaccgtt ctttctttcc tgtctggttg caaatcgtta gcgcggcagt gatcagtgga
    10861 acaattatgg tgttatttgg cggaaagatg accgatttac ctgtgacttt agtcattgga
    10921 ggattgggat acgcagctta tctatatagt ttgaaatttt ttagaatcaa atttttatcg
    10981 gaatttttat cttctttatt gattggcata gccgctatct acagcgtacg ttttggctta
    11041 ggaagcaatc aggatctaat cattatcgga tgtgtgatgc ctttagttcc tggagttcaa
    11101 attacgaacg ctatccgtga tttgctagct ggacattatg tctcaggagt ttctcgcgga
    11161 acagaagcga tgatgactgc tacgatgatc ggttttgcga ttgcttttat tttccagctt
    11221 ttttattagg aaaggaacac aagtatgggg acgatattta ttcagttttc ttttagtttt
    11281 ttagcgactg cggcatttgc cgttattaca aatgtaccta ggcgttcatt gatttgttgt
    11341 ggtctttctg gtgcttttgg ctggatgatc tactggatct gtgttcaaat aggcggatct
    11401 gctgcttttg gttcattgct tggttcttta ggagtagcat tcatcagcga tcttttctct
    11461 aaacgtttga aaatgcccgt aacgattttc aatattcccg ggatggttcc tctagtccct
    11521 ggtggtttag cttatcaagc ggttcgaaat ctcgttactg aatcctatcg agaagcggct
    11581 cattatgcgg tccaagcaat tatgattgct ggagcaatcg ctttaggtct tgtattatca
    11641 gaagtcttta accataacat ccgtaatttc aaagtaaagc gtgacgcaat catgatgaaa
    11701 cgaaaaaaac ggctttttaa ataaacgaag tgtaacaaag aaaaggttgt gaaagagacg
    11761 ttcagccttg agcgatgaca aaatatctca caaacattgc gacaagtaac cgcaggagca
    11821 ataagaagta attatgagta ttaaaaaaag ttttgtaaaa atagcttctc acatttttac
    11881 ataaaattga cttaatactg agaaactgac ttttgaacac cctctatctg taaaaaaggg
    11941 gctgcgacat gatttttgtc acggcccctc ttctttattc aaattttttc caaaacgttc
    12001 gttgataata aacgatccat agagcggaaa gtattagaac taaaccgatc acaggaatat
    12061 tcactaacca aaatggataa aacaaagcca acattccgta taagacgata agaataatac
    12121 tgatgatcaa tgaacccatc atagttgcga ctaaacccaa acttcctgaa cctctgttaa
    12181 ataattggtt aatattggtc cagttcagca gcagcaaccg ataatctcta gcgaaataac
    12241 gtagtgacaa taagtagcag ccgaataaag aaccaaatag caagctaggg ataaaaaaca
    12301 aaggcagctg aaaagacagt ccgcttaata atgcgatccc accagttaaa aaactctgca
    12361 atatccatcc gaatctgaac ttttctttta aatattggtt catagagatt ggcaaagaac
    12421 ggataaacag aaaattttct tgatccaaag aaataagatt gctgataaaa gaagtctgat
    12481 taactgttaa gaaagccaat gcgatcccta ccaggaaaac aacaccgatt aacttattat
    12541 ctaaatcagt taattttatt tcgcctccta gggcaaaagc aagaataaaa atgattggag
    12601 ttaacaagga agaagaaaga acttgcataa tcaaattagg ttcttttact aattgagcat
    12661 tgtaactaaa taaaagctga tgcacatttt ggttcttttt atgggttctt tttattttga
    12721 actcactgtt tgaatcagtc agttgctctg taagctttgg tactatcagc tgttttacag
    12781 caaggaaaaa tcccagagcc gtgatagcta gtattcccca acccaataaa ccttgttgag
    12841 aaagtggctg actggcagca tcatagaatg gcaataatat cgaaatcgga tgccgatctg
    12901 ttaactcatt ttcaatagtc cccgtttgat gattcatcca catgattcca gcaaatgcga
    12961 tcagcatcga gctgccaagc aagatactag aaactgtttt tctatgtttt ttaaacactg
    13021 ctgttcgagc caaaccaaaa acaatcaaac tagccagaca aaaaagaata atcaaaaata
    13081 gagaaaacaa aaggattgag ataatgatag taagcaaaat ccctagatca gctcgccatc
    13141 cggttaaaat aaacatgact agaagcggaa aaacaaaagg aatgatggtc aaagcaacta
    13201 ccaaaatctt tgctgaaaaa acctgttctt gtccaaaagg cagaggcaaa aatacttgca
    13261 ggtcatttcc ttcaaaaaag atattataaa tgactgatat actttgagtt aaacctaaca
    13321 taccaaaaag tgccacgtaa taagtaaaga aacctggcaa tttaacaaag ttcatagcaa
    13381 acatagcaaa tccgtaaatc agtaaaaaaa tcagccctga aaaaagattt tgagacaaaa
    13441 gatagatagt taatcgcttt cccttttttc ctttctctct tgctttattc gtgagctgtg
    13501 gattaacata taaaagatta actcgaatta attctaataa ttgtcgctta tacatacggt
    13561 ccatctcctg tcgcttgcct tccagccatt ttcaaataaa tcttttctag cgtttcatca
    13621 ggtgagccat tcaataattc actaactgcc ccctgataaa tcaattcacc tttcttcaaa
    13681 atagccagtt tatcgcataa ctgttgtgcg gtatccaata catgtgtaga aaaaatgact
    13741 gtttttcctt ttgcggcatg agccttcatc atttctttca aatcaaatgc tgcttgagga
    13801 tcaagtcctt gcaaaggttc atccaatacc caaatatctg gatctggcag caacgtgccg
    13861 atcaaaatcg ttttttgacg cattccatgt gaaaaacttg cgattgtttc attttgatga
    13921 ctatacatat caaaaagcgc tgtcaattct gctaaacgct tttctttttt ttgtgtgtct
    13981 aattcatagg cagctgaaat caagtcccaa tattctccag ccgtcaattg taaaaaaata
    14041 tctggggaat caggcacata accgattttt tgtttgatac tcaatcgatt ttccgtcaat
    14101 ttcattccat ctactaaaat cgttcccgat gatggttcaa taatactaac taaactttta
    14161 atcgtagttg attttccggc accattatgt cctaaaaatc caaaaatttc cccttgtctt
    14221 atcgagagag acaagtcttt taatgcttgt tttttcccgt aacttttact tacattgatg
    14281 aattcaatca tttctgtact ccttttgttt gttattactt tttattgtac ccttcgttat
    14341 tggtggagta aatgtcaaaa agagtgcata acaaaaaagg atagaaacga ctaggctgct
    14401 acttgggttg ttctcttcta caggaaaaca ctcttctaac aggtaagata tgagtctttg
    14461 ttttctttaa aatgattcat tttctatctt ttcttgcata aaattcttta gtttatcaat
    14521 acttctcttt cccatctcta gttgttctcc atttgcaaat gttactattt cgtttactct
    14581 gctaatagat gtaatgtttt cagtattaat gatataagct tttaaatctt tacataaata
    14641 ctcatattcg agtgattttt tatatttttt aattgtgccg tttacgagaa tctcttcatc
    14701 tttcaatact aggcctattt tatttttata acctggcatg ctcattagat aaaggatttc
    14761 atcgtaatgg atatagtgat cgctattacc ataacggaca gttgtccacc tttttttcgt
    14821 agcatgttga acaggatagt tttctttttc tttcaataag taactttgga tgcttttttt
    14881 atttttttca taagtgaatt cttttatcaa ataaccacta ggcgtaatct ttcgattaat
    14941 aacagttatt gctttattcg gatgagacgt tagataaaca attttacact tttcattctt
    15001 ttctctaagc atttcgccta gttcgatacc gctataatag gcgttcaaat cgatgtctag
    15061 gaaaaaaata tctgaggaat agatttcatt atttttaatg aattcaacaa attgtataac
    15121 gttcaatccc tgaacgatct caaatttcca aggacttaat aaaattctgg attcttccaa
    15181 aatttctact agcatttttt caaaaaccaa actatcatcc aaaataaata tacgcgttac
    15241 cataaaaagt acctccactc gatattttac tatcaaaatc atttcataca acttttcaac
    15301 atacatgtta ggaataaatt gtaattctct tcacaatctt ttccaacacc tttgaaaccg
    15361 atatcaaata ctttcaggat gctgcttcta aaataaatat caaacaaatg aatacatcat
    15421 gcaaaaaaag ccagacaacc gacgtccttg cgtgtcagct gtctgagcta tgtttattta
    15481 ttttgttctt ttaaaaagaa cgatacacat actgattttt cggagcagag actttctcag
    15541 cgtttgtaac aactacagat gggatttcac gcttgaatgg ttgataatag tctgattgga
    15601 tggttccttc tattttatac cattcatcat ttttaaccgt tactccttcc ggcatatgcg
    15661 tcaacaaacc aaagactcct gaatctgcca cacaatggat aatgccgaat cggaacaaga
    15721 agatatctgt attgctatcg ttcggtgttt ggtaaacaaa tccttcatag ctgatttttt
    15781 tgccaatgaa ttcgctaggg taattataaa ttagctccat aacttctaaa tagttttcat
    15841 ctgtgatctt gatagtttgc ttatccccat acttttcttt caactcattc atttggtcta
    15901 agtagtctga tttgttgaaa taaatactgg tatccggctt taagtattgt gtctgcatct
    15961 ccggatcccc tactgattct ttacttactg ggaaattaaa tcctttggct tctacgatcg
    16021 tagtgtcgag actaactgtt ggaaacaacg tccccacgat tactggcaaa gctagcagta
    16081 cataagcgat tccacgttga taaggttttg acaacccatg gtcatgatcg tgctcgtgtg
    16141 ccttttcttc tgcttttccg tttttgaccc acaaatacag ctgaactaaa gccaaaagag
    16201 cagataaaat cattgaaaga atagctaagt agcgataatg gacattgata tattgatcga
    16261 gtttgccaga aacttgcaag tacatcatca attcgaaata cccagataaa atcaaaaacc
    16321 gcagcatatt ttccctccta aataaataat gtgaatccaa taacgctaag ggcaatcagt
    16381 ccgatcagcg ataaaataaa gcgtcctgta aaatatcgtt tcatcatcag tagatttttg
    16441 atatcgacca ttgggccaaa gaccaaaaag gcagctacag gtgctgtgcc gaacagactt
    16501 aacaaagaag atcctataaa cgcatctgct tctgagcaaa gagacatcgt aaaggccaat
    16561 aacagcatga tgataatggc taaaatcttt gttgagccga gcgttagcat aattctagtt
    16621 ggtaaataag tctgcatcgc agctgcgatt aatccgccaa tgatcaaata acgacctgta
    16681 tcaaaaaatt cgtcgattga atgtgtcaac acatgcccag tttgatggat cagctgattt
    16741 tttggttcat tcttctcact ttgttcttct tcatggacat gatgatggtg attttctgct
    16801 actttagacg tgattttttc tttcaataca gcgtcttttt gaaaataggc aagccagaca
    16861 ccaatcacaa gggcaacaaa aaagctgcct atcacccgcc agcttgccat ctgccaagaa
    16921 ttgccaaatg cgataaaagt ggaaaaaatc acgattggat tgatgatcgg agcagtcagc
    16981 ataaaagcaa acgctgtgtg aactggtacg ccttttttaa caaattgatg aacgatcggt
    17041 acgatgccgc attcacatga aggaaagaaa aatcctaaga tcgaaccagt aaaaatagag
    17101 aagatacgat tttcaggaag ccatctcttc acacgttctg gggtcaaaaa tacttgtaag
    17161 gcgcctgaga taagacagcc taataatacg aaaggtaatg cttcaataac gatcgataag
    17221 aaaatcgtac tcatctgcaa aaccgaattt ggaataaaag aaaacatgag tttcctcctt
    17281 tttatttcta gttcattcgc tttgcaaata tacgctttta ttcaaaaaaa acaagttgct
    17341 ctgtaaattt ttacacaact cttatttttt accaaagcac ttcaattgac tttttctgtt
    17401 attcccatta aattaaggtc tcatacatat cacttctatc ataaaaggga gaataaaaat
    17461 gaaacaaacc actaacttga ctgaagttca agacatactg caacagtcat ccgttgccgt
    17521 catttatctt tcgatgccta attgcagtgt ttgtcacgct gttcgtccac gactagagga
    17581 acttctcaca cattatgata ttccggcact tcatttggat gcatttgaaa caccagaagt
    17641 agctagtaaa tttgaagtgc tgactgcgcc agttgtcctg atttttcatc aaggaaaaga
    17701 agtctttcgg caagcacggt tcatcgactt taaaaaaatc cgttatctac tagatgaact
    17761 gacagcagaa gatgattccc tttcttatga agaaatcttc cgaacaaaat aatcgttgtc
    17821 caaacagaaa aaagcgattg aacaagtgaa agttttgcct tcatttgttc aatcgctttt
    17881 tatttttatt ctgctgtatt ttcggaatgc gcatttgcgt cagaatgcaa gaaatagatc
    17941 aaggtttgca attcattcgt caaatcaaca ttttggatca atacatctga cggagcattc
    18001 aatcgagcag cggtaaagtt caagattcct ttgattcctg cttccgttaa ctgattgact
    18061 acttcttgtg ctctttcagc gggtatcgtt aaaattgcta cttcgatttg ttggatcttg
    18121 atttgttctg acatatcttc cattggataa acggggatac cgtctacgat gcgcccaacg
    18181 atttcctcat tcacatcaaa agcacaactg atacgtatgc tattactttg atggaatttg
    18241 tattttagta aagcacttcc caagttccct acaccgatca gcgcaacatt tgtcaattga
    18301 tcgtcgttta atgtctttgc aaaaaagtgc ataagatctt ctacatcata accataccca
    18361 cgttttccta attcgccaaa ataagaaaaa tctcgacgga tcgttgcact atctacttga
    18421 acggcttcac ttaattcagt tgaagacact tttttctttc cagcattatt taaaatcctt
    18481 aaatagcgat aatacaaagg cagtctttta gctgtcgcct ttggaataat atgatctttc
    18541 acgaaatgac ctccacgtat tgtctaatta catctttgat ttctccatca tagcagtatt
    18601 tataggaaaa aatcaattcc attgctcaga tattgtgaaa ttttattctt tattcattta
    18661 tttatcattt ctgcaaatct cgcagtataa tggaatttat ggtagactac taattgaaaa
    18721 atagtttttt atttgaggag aattcacaaa gtagaaaaga ggattccatg atattattac
    18781 aagccaatca gattgctcgc cattttggtg cagaagtctt atttgaaaac atacatttag
    18841 aaatcagtac aggcgctcgt gtcgctttag ttggcagaaa tggcgctgga aaatcgactt
    18901 tattaaagat cattgctgga attgaagcac ctgatgaagg aaagatcgct aaaaataaaa
    18961 cagcgactct cggatacctc gctcaggaca caggacttga ttccaatgaa acagtttgga
    19021 acgagatgct gaaagctttt gaacaagtta gacaaatgga agagcggatg cgcgagctag
    19081 agattttcat tagcgaaggt acgccagaca cacctgctta tagttcttat ttaaaagaat
    19141 acgaccggct gcaacacgat ttttcagatc gaaacggcta tggttatgaa aatgaaattc
    19201 gttccgtatt gcatgggttt aaattcgatt cttcatttta tgatcagaaa atcgatactt
    19261 tatcaggcgg acagcgcaca cgtttagctt tagctcgcat gcttttgcaa caccctgaca
    19321 ttttgattct agatgaacca acgaaccatt tagatatcga gacactttcc tggcttgaga
    19381 attatctgca aagctatact ggtgcattat tgattgtttc ccatgaccgt tattttttag
    19441 ataaagtagt caatgaagtc tatgaattaa gcagaagaaa aatgcgccat tacaaaggta
    19501 actattcgaa atatctagat ttgaaagcag aacagttagc aagtgaatgg aaggcttatg
    19561 aaaagcagca aacagaaatc cataaattag aagacttcgt tgctcggaac cttgttcgcg
    19621 cctctaccac aaaacgagcg caaagccgca gaaagacatt agaaaaaatg gaccggctgg
    19681 atcgtcctca aggggatgaa aaacaagcaa actttctatt cagtatagaa aagccttctg
    19741 gaaatgtagt cttgcaaatc gaagatgccg ctatcggata tgataatgtc accttatctg
    19801 aaccaatcaa tctagatatc cgtcgtcaag aagcgattgc tctagtaggt ccgaatggta
    19861 tcgggaaatc gacgttgctg aaatcaatca ttcaccagat cccttttatc cgtggaaaag
    19921 aaacgctagg gacaaatgtc accatcggct attacgatca aggtcaagct gatctgcata
    19981 caaacaagac gatcctagca gaattgtggg atgagcatcc taccactcca gaaaaagaga
    20041 tccgaagtgc tttaggtgct ttcttatttt ctggcgaaga tgtagaaaaa ccaatccctc
    20101 tgctaagtgg cggtgaaaaa gcacgggttg ctttagccaa gttagcgatg aacaaagaaa
    20161 attttttgat tttggatgaa ccaacaaacc atttagatat tgacaacaaa gaagtattag
    20221 aaaatgcatt gatcgattat gacggcacac tgctttttgt ttcccatgac cgatatttca
    20281 tcaatcggat cgcgacaaaa gtcgtcgaac tctctgaaaa tggcagcaaa gtctatctag
    20341 gagactatga ttattattta gagaaaaagc aggaagaaga agaaatcgcc gaattgttgg
    20401 cacaagatga gccagaatct gctcctgctc ccacgaacaa atctgattat taccaaagca
    20461 aagaacagca aaagcttatc cgtagccttc aacgaaaaat tactcaggtt gaagaagaaa
    20521 tggcgcggat cgatgaatgg atcgatcagt tggaacaaga aatggtcgcg ccagaaaacc
    20581 tggaagatca cgtaaaactc aatgagctga accaagattt agaagctgct agacaagatc
    20641 aagaaacaaa attgacggaa tgggaagaac taagcttgga attagaagaa tatgaaaatt
    20701 aacgaaaaaa ccaaaacaga aaccattcat gctgttttgg tttttctttt tttattttat
    20761 caaacaaaaa ttccatattt acctttttca ccaataagaa gaccaattac tagtcattct
    20821 cactaaagct tctaaataat agtaatcacc ccaaatacaa ggtgtattta ccccattatt
    20881 tttgggctta ttataaacag actgtgttaa aaagccatct tcttcttccg ttgtagcata
    20941 tctttctttc aattgtcgca taattttcca aacagcagac tggtataagt tcttttctgt
    21001 tttgtctaat agacctgatt ctattaattc taataatcca caaaccatga tcaccatact
    21061 agatgtatct ttttcttctc tgctagaatc atcaaagatc aaatcccaat aacaaatggt
    21121 atcttctggc aaccgattca taaaataatt ggctaatatt ttgcttaaat caaaatattt
    21181 ttgttctttc aaataacgat aacttaatgc aaaaccgtaa atcccccatg cttggcctct
    21241 agcccaacag gaattatcac tataaccttg atgcgtactt ccatatcttg gttttccatc
    21301 tttgacatcc atataaaatg tatgataagt tgaaccatct tctcttacta aataatctgc
    21361 tgcattttga atatgagcct gagcagcttt tttatatttt tgatttccag tttcttcact
    21421 cgcccaatac aataatggaa gattcatgca acaatccatg atcattctac cttgttgtga
    21481 aggatcacta ggatctcccc aagcttgtat aactcctgcc ttctcccaaa aacgctgaag
    21541 taatgtttct gcagcatcaa ttgaagcttc cttcattttt cgatttcccg ttattttata
    21601 atctgctacg gttgataaag aatacaaaaa accaagatcg tgatgattag tgataatttg
    21661 acagtcaatc ctgtggataa aatctatatc tgctttttca gctgtttgtt gaaataaagg
    21721 atcacccgtc ttctcaaata acaaccaatt aattcctgtc caaaatcctt ctgtccaacc
    21781 aatattgtct cttaatggat aacgtccttt tataggacac tcgcttggga attgatactg
    21841 actgttactg atacgtttga ttaacctctc cgtgtgtctg cgacaacgct cgatctcatt
    21901 attccattct ttttccatta attctttata acattcattt atccattttt ctggatgtat
    21961 tatctcttca aacatcactt tatcaagcat ttccccacca gcctttagca taattttagt
    22021 ctttttttat tgtttttatt ttggatagtt agcccggttg tcccatcaaa atcaattttg
    22081 ggacgattgt tccatacttc taaactataa tcccgatctt gatgagcaac cactcctgtt
    22141 ccgagccaat gggttccttt tccaaatgta ccctctaatc caggaataaa agtgttttcc
    22201 catgtcatca agtttgtatt gggcaaaccc ataatagctg tggattttcg tttaatagta
    22261 tcgtcactta aacagataat acctgaaaag ccagcttcac tcaccctata gttttccttg
    22321 tcttcttcta tgattattcc ttctttctca ggagaacatt caatagcaaa ccctgtctca
    22381 tatagaacat attctgtatc taattctact ttgtgtacgc gaatctgcca gccttcaaaa
    22441 ggaataagcc aagttctgat ttttgcctgt aaagtaggtt cccatgtaga ggcaagtacc
    22501 tctttcttac atacaatgtc tcgtacatta tttcgaacga tgaactgtcc ataggttttg
    22561 atgctctcat tcttaaaact ggtcgtgata tatgtgtccg ctcgctgaat ccctagcatt
    22621 gaatccattg ccatttctgc tttcaattga ttcgctcgcg gtatcgaaaa accaaattga
    22681 ctagaataag caaattttaa atatttttca ttggtcaatg tatggaaatt tggccctaat
    22741 tgtcccgcat tcaaaaaaaa tgtatgtcca ttatcatgtt gtatgagcaa attggcttct
    22801 gaaagaagtt tagttccttc acgaataggc ataggcattt ctttagccgt ccaatactca
    22861 tggtcttcag gtagcgccaa caataaaaaa atcttattca gccataatgg agataacgta
    22921 ctattatagg gttcagtcaa tgtcaattga gggtaggtat aaccaatatt caggatacct
    22981 ttttcatcaa atatttcttt ttgcatccac catcttatat tccgattgac taaccccttg
    23041 atttcaccta atttaaaagg caaaagatcg gcccacaaca tagctgacca aaagcttgaa
    23101 actgcaaaac gataaatcat gcttcgacca aaaggcacat ttgcaccatc ttccgcaaaa
    23161 aaatgaatat agtctctaga aaaatcaata gctcggttct tatatatctg gctaatgggc
    23221 ttttctggtt ctagtacgct ataaatcaaa ccataataat gaaatgcaaa aggattataa
    23281 taatcttggc gacctcttga actgtctcga taccagccat tttgaagata acaattttct
    23341 atattatcaa atgcttcttt cagtgctgat tctttaactg gataaccaaa tttttggata
    23401 attgttccga caattatctt aaaaaattgc caattaccat ctgcacattc atattcaaaa
    23461 atttgcgaga accaatgaat aatctggctt ttttctcctg tatctaattt tttccataat
    23521 aattgttcat gatgatataa cgctaataac attgcgggca tttctaccat acgctggtct
    23581 ctttcatgta tttctcccca ataatcggga tgggaaggat ctgttccagc ttttattcct
    23641 gatagaatta atttcaacca tcgttcatct aattggtcaa ttgcgggccc cgctccccat
    23701 aaaacccgag agaatccttc cattcctgca attttatttc catatcctac cccttgatgt
    23761 atataatcaa tttgtgcgcc acttgctcga taaaacggct ggagtggttg atttatcttt
    23821 ttaaataagt caaagtaatc ctgtctggtc cttaatagtg gtactggatg tttcattgta
    23881 ttttctcctc tttttactac caaatagcta attccgactg ttctaatcga agtaagcctt
    23941 caataaaaaa ataatcagaa tagatcagtg gcacattata agtcagttct ctatggtatg
    24001 cttctgctcc gcctccaatg atcccatctt catccatatt ccaatttgca tagtgctttt
    24061 ctgtcttttg gagtatattg atagcagctt catgatacat ttttgatcgt acagaggaaa
    24121 catatcctgc taattccaga agaccgcatg cagcgcatac tccagcactg gtatcaatat
    24181 tcgaatcttt tacagcaaaa tcaatgagag gaatataacc agttttttcg attttctgaa
    24241 taaaatttac tgctatcttt tctgctatag ctagatagta cggatttttt gtgtaacgat
    24301 aacttaacgc gaatccatat aaagcccatg catttcctct gctccaacaa gaattctcat
    24361 tgtatccttg cccgccaagt aaccgaatat atttccctga ttctgcatca aattctgcga
    24421 tatgcccggt tgatccatct ttcctcacta gatacttagc tgttgtatct gcatgctgcc
    24481 gagcaatttt agagaacctc ggatcaccag tttccctcga agcccaatac agtaatggta
    24541 aattcatcat actatcaata atcatccaac caggtttatg gtcattccat gctactaaat
    24601 agttccctga aatattatag cgtccagcaa gtaccgtagc tgcatgtaac cctagttgtc
    24661 gactatgttt gttttgagac tgttgataat gtgcaatagc cgatggtaag aataaaaaac
    24721 ctacatcatg atgaacgtct gaaaaatggt acagggcaga ttctaataat tgttcttgtt
    24781 gtattgctgc ctctttgaac ttttctcgtt ttgtcttgtg aaagagctgc cataaaatac
    24841 cactccaaaa accattggtc caccagtcga ttcctctgtc ctttattacg tctgtatatt
    24901 ttccgttgta agaaatatat ggaattttat tccccattct gtctaattcc gcatctatct
    24961 tttcagaagc tacattcaaa atttttcttg accattcctt cgtctgttca ttcatggata
    25021 tcccgccatt cttttaattt ttatgaatgt ggagttgctc ctagaacgaa ctagatggtt
    25081 aacattcgtt atttttttta cttatttgca gctccaatgt cattctcctt aaatttagac
    25141 tgcgcccaca cataaatcaa gaccattggg atcactgaag ttgctaaaat tagcataatt
    25201 gcgccataat taattggctc acctgtacta gaaaccgtca tatttttgat aattaaggga
    25261 accgtatatt tatctttatc tgaaagcatg actaatggaa atagatattg attccaactt
    25321 cccataaaga taagtaaacc actagtaata atcgtaggtg ataaaatcgg tacagcgatt
    25381 ttcgtaaaaa tcacccactc attggcacca tcgattctgg cagcctccat cagtgctgta
    25441 ggaaattgac tgctatattg tcgcatcata aacactgaag ttgtactggc aatcgaaggg
    25501 atgatcacac ctgcataagt attcgataat cctaaacgac tcataatgta aaataaagga
    25561 atcaatgtgg taaaaaaagg caccatccta gatagcataa ctgtattgaa caacagttta
    25621 ttccccataa aacgatactt tgctaatgca taacccgcca ttgtagaact ggctgtagct
    25681 aaaactgtac tgattacagc aataacaatc gaattccaaa taatcaaggg ataattatat
    25741 ctcgccatga tggcttgcca gttttttaat agcatatccc caaacttgaa attgattccg
    25801 ccacttaaaa tttcttgatt actttgcgtt gaagctacaa tcatatataa atatggcatc
    25861 aaaaatatta aggcaccaaa cacaataatg acgtacgtaa aaattctgct ttgtatcttt
    25921 tgtttagtca tttttatcac ctccgaattt aaattgaata gccgataata taacagatac
    25981 aaacaccatc gtccaggcaa tagcagaggc ttttccaaaa tcaaccattt caaaacttgt
    26041 tttatataaa ttgactccta cgaccaaagc tgaattttct ggtccattgg aagtcccaaa
    26101 gaatatattt gggatattga aattacccaa gccattgata gtggctaaaa ctgtggccat
    26161 taaaatgatt ggctttagca tggggatcgt taataaaaag aattgccgaa atttcgatgc
    26221 tccatctatt tcagcacttt cgtatatctc tttagaaatc ccttgaagac cagctaaaaa
    26281 taaaatagtg ttatatccta cactaagcca aatcatcgtg atgatcattg atattcgtat
    26341 ccaaaaagga tttgttaacc actgtacacc gttaattcct agcgcattca aaaatccatt
    26401 gaataaccca gtcggattga aaaaagtcat gaatacggaa gctacagcca ccgttgatgt
    26461 aatggttgga atataataga cagttctaaa aaatcctttt ccttttaact gtgctgaatt
    26521 caataaaaca gcaaagtaaa tagctaatag catcgttatt ggaattgatc ctgccatgat
    26581 aatcaaaaca ttcgtgtaag atttaataaa caatggatct cggaagatag ctagataatt
    26641 gtcaaatcca acaaattcaa atgagtagcc aattttcata aaactaaaag caaacgagat
    26701 aatgatggga atcaaaatcg tcacaataaa gaaaataaca aaaggtgtga ccaatccata
    26761 agcacttttt ttatgagacg tatctgttgc ttttttcttg ctgttcttta ttgtcttgct
    26821 tggtaacgaa gattgcatca tcgttaagtc ctcctaatat tttttaatca ccagttaatt
    26881 ttacgccgta cttatgagca aaattttctg cttcttttag atatgaattt tcaaaatcac
    26941 ctgtttgcca atagttatat gcagcggtag tcaaataaga cgtagcatct gaataagtgg
    27001 ctggtaaaac agtatttgat gtattttcat tgattttatt caatagttca tagtattttt
    27061 gtcctcctta gtagtcaaac tttttctcag ctgcttctgt ttgataggcc aatgagttag
    27121 atgtagccac gcccagctcc agtgcgttgg ctaagcattc ttcatctgtc agcataaatg
    27181 taagaaattg ttgtgccact tgtggatttt ttgcatcttt agggacataa aaacttgatc
    27241 ctcctgatac tgcacctttt cctggatttt cttttgaaaa aggaatcagt ggagccatgc
    27301 gccatttgtc agatgcatca ggaaaattca aagacatatt cgtagcatac cagccgccag
    27361 caacaaacat tgcagattct tgaaatgctt tctcagcatc ttgtgcaccg tagaaatgta
    27421 caatatctga gtcgatcatt tctttgatta actttgcagc atgtttcgct tctttagtat
    27481 tcagacggat atttccttct tcatcaagca aagattgatt ctgctgggct agcatgttga
    27541 taaacaattc ttggtcccca gattctggca aggcaattcc aaaactacca gttttttctt
    27601 ttaatgcttt tgccacctgg atatattctt cccaagaact gatttcttca aaatctacac
    27661 ctatttcagc aaattttttt ggttgataat aacaaagtgc tggggtaaaa tcactagccc
    27721 aaggaataat cgtttcatta ctcgtttgtt gacgcaaaat attcaatttt tgatcataaa
    27781 actgatcgcc atatttgtca taaaatccat attcatcagc actataaaaa gcatcttgaa
    27841 atttttccag cacacctgct gctgaagtgt ctgccatgga gataatatcc ggcatatctt
    27901 cgtgtgttac caattttggg gtgatcattt gtactgttgc tgttcccgaa gctacattca
    27961 caatttttaa gtcaatattt gctccagttt tgcggttata gatttttgca gcattttcaa
    28021 catagtttgt cagtttgccg tttgcgacat atacttctaa tgtagtggct ttttttatca
    28081 ttggatctgc gatgtttgct gttacttctt cgttagtttt tccacatgca cttaagctga
    28141 ataatagggt aatgtttagc aaagctatca ccaatttgta tctttttttc atgtctatcc
    28201 cttctttctt ttcaagttct tatataaaac gctttcactt a
//
LOCUS       DACCXM010000022        27911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCXM010000022 DACCXM010000000
VERSION     DACCXM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.023"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(475..2367)
                     /locus_tag="IVY61_000846"
     CDS             complement(475..2367)
                     /locus_tag="IVY61_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969978.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2364..4091)
                     /locus_tag="IVY61_000847"
     CDS             complement(2364..4091)
                     /locus_tag="IVY61_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7969979.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4107..4757)
                     /locus_tag="IVY61_000848"
     CDS             complement(4107..4757)
                     /locus_tag="IVY61_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7969980.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4961..5257
                     /locus_tag="IVY61_000849"
     CDS             4961..5257
                     /locus_tag="IVY61_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7969981.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5449..6069
                     /locus_tag="IVY61_000850"
     CDS             5449..6069
                     /locus_tag="IVY61_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP7969982.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6161..7327)
                     /locus_tag="IVY61_000851"
     CDS             complement(6161..7327)
                     /locus_tag="IVY61_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7969983.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7339..7692)
                     /locus_tag="IVY61_000852"
     CDS             complement(7339..7692)
                     /locus_tag="IVY61_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7969984.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7820..8842
                     /locus_tag="IVY61_000853"
     CDS             7820..8842
                     /locus_tag="IVY61_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP7969985.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9281..9715)
                     /locus_tag="IVY61_000854"
     CDS             complement(9281..9715)
                     /locus_tag="IVY61_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7969986.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9825..11684)
                     /locus_tag="IVY61_000855"
     CDS             complement(9825..11684)
                     /locus_tag="IVY61_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7969987.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11802..12962)
                     /locus_tag="IVY61_000856"
     CDS             complement(11802..12962)
                     /locus_tag="IVY61_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP7969988.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13269..14693)
                     /gene="araA"
                     /locus_tag="IVY61_000857"
     CDS             complement(13269..14693)
                     /gene="araA"
                     /locus_tag="IVY61_000857"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP7969989.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14709..15407)
                     /locus_tag="IVY61_000858"
     CDS             complement(14709..15407)
                     /locus_tag="IVY61_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7969990.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15364..17025)
                     /locus_tag="IVY61_000859"
     CDS             complement(15364..17025)
                     /locus_tag="IVY61_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP7969991.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17243..18643
                     /locus_tag="IVY61_000860"
     CDS             17243..18643
                     /locus_tag="IVY61_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP7969992.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18895..19974)
                     /locus_tag="IVY61_000861"
     CDS             complement(18895..19974)
                     /locus_tag="IVY61_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7969993.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20060..21019)
                     /locus_tag="IVY61_000862"
     CDS             complement(20060..21019)
                     /locus_tag="IVY61_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP7969994.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21031..22554)
                     /locus_tag="IVY61_000863"
     CDS             complement(21031..22554)
                     /locus_tag="IVY61_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7969995.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22564..23127)
                     /locus_tag="IVY61_000864"
     CDS             complement(22564..23127)
                     /locus_tag="IVY61_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7969996.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23133..23996)
                     /locus_tag="IVY61_000865"
     CDS             complement(23133..23996)
                     /locus_tag="IVY61_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7969997.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(24010..24906)
                     /locus_tag="IVY61_000866"
     CDS             complement(24010..24906)
                     /locus_tag="IVY61_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7969998.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25081..26352)
                     /locus_tag="IVY61_000867"
     CDS             complement(25081..26352)
                     /locus_tag="IVY61_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7969999.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26342..27859)
                     /locus_tag="IVY61_000868"
     CDS             complement(26342..27859)
                     /locus_tag="IVY61_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7970000.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8148 a         5876 c         4848 g         9039 t
ORIGIN      
        1 gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaaaagtc
       61 gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttctacatt tttgcataaa
      121 tttggcttaa taccgagaaa ctggcttttg aacaccgttt accaagaggt tgtgagcctg
      181 ccgtttaact ccgagtcaca aggagcaatt tcaaaaaaca gcttctcagg tttgttgact
      241 attgtgactt gtgaccgagg agttggcagg agaacaccgt ttaaggaaga ggttgtaaaa
      301 tcagcggttt gacctgagta ttagagatac aaaacggaaa aatagcttct catattttgc
      361 cgttttttga gctaataccg aaggacagct gattgaacac cgtttattcg gaaaaaggga
      421 ctgtgacacg acttttgtca cagcccctaa aaaaaataat aaatttgtac tattctatga
      481 agcttcttct tcagaaaact gactgttata aagatctgca tagaatccgt ctttttccat
      541 caatgtatcg tggtttcctg tttctacgat cgaaccatga ttcatcacaa taatgttgtc
      601 tgcatcgcgt attgtagaca agcggtgagc aacaacgaaa cttgtccggt tttccaatag
      661 gcggttcatc gctttttgaa tcagtatttc tgttcgtgta tctacactag atgttgcttc
      721 atcaaggatc aggacatctg gatttgctaa gaatgcccgc gcaattgtga ttagctgacg
      781 ctgcccttgc gaaatattgc ttgcatcctc gtttaataca gtgttatatc cttctggcaa
      841 tttacgaaca aaatcgtcta cgtgagcagc tttagcagcc tcaatgattt gttcgtctgt
      901 tgcatcgtcg ttcccatatt tgatattgtc atagatactt cctgtaaaca gccaagtatc
      961 ttgcagtacc attgaaaact gtgcgcgcaa ttcatcacgt gaaagatcac gggtatctac
     1021 accatcgtaa cgaatactac ctccagaaac atcgtagaac cgttcaagaa ggttgatcag
     1081 tgtcgtcttc cctgcaccag tcggacctac aatagcgacc atctcaccag gtttgacatc
     1141 caagttaaaa tcagtcatta gcaagttatc tggtgtgtag ccgaattgaa cattttcaaa
     1201 acgaactttg tatggtgtat ctttttcttc tggaaggcca gatttttcat tggacatctc
     1261 ttcttcatcc aacacttcaa atacccgttc ggctgaagca actgttgatt ggatagtgtt
     1321 catcaaactt gcaagttgag tgattggttg agagaactgt gttgtatatt gcaagaacgc
     1381 ttgaacatcc cctaatgtca tgttcccatt tgcaactttt actccgccta gtacagcgac
     1441 gaacacatag cctagatttt taacgaaatt catcaacggc atgatgattg ccgaaataaa
     1501 ttgagctttc caaccagcgg catacagatt ctgattttct ttttcaaata cttcctggtc
     1561 tttttcagta tgattgaatg ttttaacaat ggtatgacca ccatatgttt cttctacttg
     1621 gttattcaat aaacctaaac ttttttgctg atctgcgaaa aacacttgtg atttcggcgc
     1681 tacgatcatc acgatgatca agcttaaagg gatcatcaat aacgcgacta acgtcaattg
     1741 ccagctgatt gaaagcatca tcacgatgac cccaatcaat gtgactgcac tagttacaaa
     1801 ttgagtcaaa ttttgctgca gcgtgcttgc gatgttatcc atatcattga ttgctcgaga
     1861 catgatatcc ccatttgtat gtgtatcata ataggaaatc ggtacacgat tcattttggc
     1921 ttccaaatca tgacgtaatt cataaactgt tcgttgggaa acacgtgtca taataaattg
     1981 ctgtaaaaag ttgaatacag cagaaatcaa atacatcacg attactgtcg caatgatctg
     2041 cgcgatttta tcaaagtcta tcggaaattt tgttacttgt tggccttgtt gcatctgttg
     2101 agcgccagcc attacacctt tgaatatttc tgtggttgct ttccccaaga cctttggtgt
     2161 ttggatttgg aagatcacgg ctgctattgc tagtacaaat acagcgatga tcgcaaccat
     2221 acgtttagac atatagccaa acaatcggcg taccgttccc cagaaatttt taggcttttc
     2281 accttttaca ccgagatttc tgccaggtcc accatgtcct ggaccgcctg ctggtccatg
     2341 agagggacgt gtgtttgttt tactcatgct aagtcctcct ctcttagttg tgaatgaacg
     2401 atttcttgat aagtttcgtt tgttgctaat agttcttcat gtgttccttt gccaaccagt
     2461 tttccttcat ctagcacaag gatcatatct gcatccatta ctgtacttac tcgttgagca
     2521 actaaaacgg tgatactttc tttcatattc gttttcaatg cttggcgaag attggcatcc
     2581 gttttgaaat ccaacgctga gaaggaatca tcaaacacat agatatctgc tgttttgact
     2641 aatgcccgtg caattgccag acgctgtctt tgtccaccag agaagtttcc tcctccttgt
     2701 tccacatggc tatctaagcc atcttccatc tctgaaacaa aatctttagc ttgtgcgatt
     2761 tctaatgctt tccaaatcat ttcatctgtt gcatccggct taccatactg catattgtct
     2821 cggatcgttc cagagaacag aactgctttt tgaggaacaa agcccattaa ttctcgcaaa
     2881 ttatattgag tcatgtcact aatgtccgtt ccatttattt ggatcgaacc agattcgatg
     2941 tcatataatc gagggatcaa attgactaag gtcgttttac ctgaaccagt cccccctatg
     3001 attgcgacga tctcgcccga cttggcttgg aaatcaatat cttccaatgc aagtttttct
     3061 gctccatgat atcgataatt gacatgattg aaggccaatg ttgcgttttt tccagcaaaa
     3121 gaaactgttt taggattttc agggtcatgg ataccgattt tttcatccaa cacttcatta
     3181 atacggtcag ctgaagcttg tgcacgaggc accatgataa aaatagcgga aagcatcata
     3241 aagctgatca agatctgcat cgcataagtc ataaatgcaa tcagattacc gacttccaaa
     3301 gtcatatctg ctatgtagtg tccaccaaac caagtgattg ctacgtttgt accgctcatg
     3361 atcaatgtca tcattggcat catcaatgcg acgatcgtat taactttgat tgccgtattt
     3421 gtataatctt tattcgctaa atcaaaacga ttttcttcaa agcgtgtctt atcgaatgct
     3481 cgaatcactc tgacccctgt caatccttct cgaaatacca ggtttaaacg gtcagttttc
     3541 ttttgcatac ttttgaataa tggaacagca aagtacatga ttccgccgat caaaataatc
     3601 attactggga tgacaactaa gaagattttt gtcaactgat gatctttttg ataagcaagt
     3661 atgctcgcgc caatcaacgt gattggtgca ttgatcatca tacgtaaaaa catttgtgta
     3721 accatttgga tttgagtcac atcattggtc gttcttgtga tcaaagaggc cgttccatat
     3781 ttatcaaacg catcttttgt cagactctct gattttttat agacatccgt acgcaattgc
     3841 ttacccagtt tctgtgattc acgagtagca aaaaatgtat ttgcgatagc agctaagata
     3901 ctgatcaaag aaaagccgat catcacaaaa cctgtatgcc aaatataatc gacatctcct
     3961 tgcgctaccc ctttatcaat aatattggag gtaagtgtcg gcaaatataa atctgccagt
     4021 acttggatca ccatgaaaac tgcagctgca attgctgaag tcaaggagat ccgttttacg
     4081 atttttagca tctagttccc ttctttctat ttatatactc cgtttttcag ccaagaaagt
     4141 ttcaaattga attcactgat tgcttgctct actgagtaat cttcagatgt tgaaaataag
     4201 cgatacgaat gcgcaattgt tgtaaataca gtattcatca ctaattggat cagtaattcc
     4261 aattcatgat cattttttac tttcagcttc gtccgatcga tcaattcaag caactctttt
     4321 gcatcttgtt tgtgttcttt atgtggatcg tgaggcgttt tttggcgact cgtatgagga
     4381 gcgacttttc ttgaggcacg gtaatccatg ttcataaaca agttacgata aaaagaagca
     4441 ttctcgcctt ccaaaatctc ataaatcatt ttagaaaaat aggattcgaa tcctttgaat
     4501 agatcaccat cggattcttt cattagttgt aacatatctt tagagctgtc acgtctcatt
     4561 gtctcaaagt aataaaaata aagatcctct ttatcttcaa aatactgata gaaactgcct
     4621 ctagggatat ccgctaattt gacgatatta gcaatcgaag cttcttttaa gggtactcta
     4681 gaaaactcga ttctcgcagc ctcgattagt cttttttgtt tttctttagg caaatggaaa
     4741 aatgtctgtg tcggcatatg gattctcctt tctttcaaga tgacacattg tcatatgacg
     4801 ctatgtcatt ataacacctc ctacctgttt tgcaagacat ttgcatagtt tttttcatta
     4861 tcatccgata cattaaaaaa gtgataagag gaatccttcc atttgaatta atgagagttg
     4921 cccattaaaa tagccacata ctaaacaaag gatgagataa atggcccaat caattagtgt
     4981 ccaagacttc aataaattgc ctctcgatgc tgacagcatc gttttagata caagaaatac
     5041 cttagattac acaaaaaacc acttaagcca ctcgctttct atccccgctg ctatcttgcc
     5101 aagacaatta aaaaatctag atccaaagaa actttattat attctaagct atacaggcag
     5161 gcgatcagaa gcattagccg atcaacttac tgctaatggt tttcgtgctg tttatatcat
     5221 tggtgggatg gaagctcttc agtcgagtgt tgcttgaata ttgcttgtta gattcaaaag
     5281 aggctgtgac aaaaaatcgt gtcacagcct cttttatacc ctgtcttttt ttagttattt
     5341 attcttcaac cttatcaata tgacttgata gccttttctt tttcgagtaa actgattttg
     5401 ttagtcatag ttataacaat catagcaata aaaggagaag gtaagcatat ggcatctgtt
     5461 ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa ggcattgacg
     5521 tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt tttggaactt
     5581 tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga agcattacgt
     5641 gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc tcgtttcaat
     5701 gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc cttatggaat
     5761 ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc tggaaaaacg
     5821 ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc actgcacatc
     5881 caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt aaaaggtatt
     5941 ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat cgattatgac
     6001 cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc tttaggtaaa
     6061 tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat cttcaaagca
     6121 gcatttgtgc cttggatatt ttcccatctg tctctttttg ttattctttc tgctttttca
     6181 acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca tgagtaatca
     6241 ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt ccgaacaatt
     6301 tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac tgattcccta
     6361 gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa acgatcagta
     6421 ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga atcatcgcta
     6481 aaaatgatcc taagtaaggg atcaggttta aaaaacccgc aataatcccc aacgtgacag
     6541 catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca gcaaaagcca
     6601 cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc agcacattca
     6661 acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct aattgtttac
     6721 cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact aaaatagtcg
     6781 ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata tcttggatca
     6841 tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg cgaaattggt
     6901 tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct acatattgag
     6961 ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc actacgctcc
     7021 cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa agccttggta
     7081 cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag attcctgcta
     7141 aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac aaatatgaaa
     7201 cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag gctgtgacta
     7261 cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc ttattttttc
     7321 cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa tagcaggcag
     7381 tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac aagcatcaaa
     7441 aatcaatgat acgtgagcta ctgtctttaa attcccattg gccatcggac cgacataatc
     7501 gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag aaaaaggttc
     7561 gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg ctgttttatc
     7621 gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt ctgtaatctt
     7681 tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa cacattctca
     7741 ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat ttcattacaa
     7801 ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata cgcgtcgcga
     7861 aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag ccaacctttc
     7921 attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg gcaatcttta
     7981 cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg attttcattt
     8041 tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg atgaagcacg
     8101 tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc atatattagg
     8161 agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag gtcctcataa
     8221 aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac gattagtcgt
     8281 atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg gcaaattgaa
     8341 tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg tctttcatcc
     8401 aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg tgctttctta
     8461 taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc cagaagaatt
     8521 cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt tcctttatgc
     8581 ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg gttcccacat
     8641 tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg cactagatcc
     8701 aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat tatatcgccg
     8761 tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac cagaatgtgt
     8821 ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc aagacgtatt
     8881 agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg aagataaaga
     8941 gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact ttgaacaata
     9001 tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac atcaggacaa
     9061 gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga gatggttaga
     9121 ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt gttggttata
     9181 ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata ttacatttat
     9241 attagataac tctagttttt attgttatca aagatttctt ttattttttc aacaacaaat
     9301 tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt tttgcttcag
     9361 atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg atcatttctt
     9421 cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg cccattgctt
     9481 ttgctggtgg acgcaaagta ctgactttca taagcattgc ccgatatttt tttgttcctt
     9541 cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt gtttttcctt
     9601 catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg attgcttctg
     9661 tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt aacatgttcg
     9721 ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc ctcaaatgac
     9781 aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc gtgctcaaag
     9841 aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat caattgatat
     9901 tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca ctgtccgttt
     9961 tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa aagcagctga
    10021 taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa ataaaacgga
    10081 ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc gaacataaga
    10141 aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc tgaatagatc
    10201 cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt cttcaattct
    10261 tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata attctctaag
    10321 tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc atactttcca
    10381 tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt tttttcttcc
    10441 ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg attattctct
    10501 atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc taatgtctga
    10561 taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt cgtctggtat
    10621 gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg tacaagcatc
    10681 gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt caatgcttca
    10741 aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc actatatgtt
    10801 tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac gccgtttaat
    10861 cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga ctcttcctct
    10921 acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc tttgatcttt
    10981 tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta caaaaaccca
    11041 ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca tagtgccgtt
    11101 ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat ataccctagc
    11161 agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct tccttttttc
    11221 aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc agctaagatc
    11281 actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt gatcatcttc
    11341 aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt tgcaaaacct
    11401 aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc tgctagtgca
    11461 atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt ttccgtatgc
    11521 caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca ccactgcaaa
    11581 aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt tccttttaaa
    11641 atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt gctccttcat
    11701 ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc gaaagtaaag
    11761 aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct ttctggtttc
    11821 tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt cacttgattg
    11881 agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc attcttatgg
    11941 ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa tatctctgct
    12001 aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg gaaaaacatt
    12061 cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata atcttttaag
    12121 gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc ccctagatga
    12181 ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc agtcaagtat
    12241 ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac aatttcactt
    12301 cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc ctgatcgatc
    12361 aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga ggtagaaaga
    12421 actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat agaataaccc
    12481 aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat cgctccaaga
    12541 ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact tcttttgttt
    12601 tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact tgcttcgatt
    12661 atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc attagcattt
    12721 acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac gttgatcgtg
    12781 ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca ttcatctttc
    12841 actttcgtac tacgaataac tattccttcg gctggcaaat catctattat tttgaaatat
    12901 tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg aacgtgatac
    12961 ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac aactgtttac
    13021 catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag tgataaaaat
    13081 aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat tgagggtgag
    13141 atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa tttcaaaaaa
    13201 gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg ctttttcaca
    13261 acctcttttt atctaataac cactgtttcc aactcaacta attttgccca cgtttcaatt
    13321 tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc ctgaatccat
    13381 gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc aacaggtaaa
    13441 ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag tttataatgc
    13501 gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc aaatacaaga
    13561 cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct tggtttcgtc
    13621 gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc ttggccacta
    13681 gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc catgattttc
    13741 agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc gtatccttct
    13801 gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag gtcttcaaaa
    13861 ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc gatttcctgc
    13921 tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc atagtcgaag
    13981 gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga tacgcgatcg
    14041 attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa ttgaatctgt
    14101 gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc aaaacgagcc
    14161 acttttattc caaaactttc attataggct actgcaacat ccatccaatc agcaatctct
    14221 ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt atttaatcga
    14281 gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt catgaaatcc
    14341 atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg aagcaatggt
    14401 ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt atgcatccaa
    14461 gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt tgtgatcgta
    14521 tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc attttcattc
    14581 agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc ttccccatat
    14641 aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa cataattagc
    14701 cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg taaataatgt
    14761 ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct ggtaaataaa
    14821 ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg cgcatctttc
    14881 ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa gggatcaatt
    14941 ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta cccgctgtcg
    15001 atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt atctgcatgc
    15061 gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc ccatgtcgag
    15121 tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc atgagtaggc
    15181 gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc cacaacgacc
    15241 atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac gaaatatcgt
    15301 gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc gtgtttaggc
    15361 aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt ctatcactcc
    15421 tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac ggtaacgtct
    15481 cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga ttccttggtc
    15541 ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc gatccatcat
    15601 ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt ccatcactgt
    15661 aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg ttttaaaaat
    15721 ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata tatccattcc
    15781 gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg ctaaatcaaa
    15841 acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg taatgttttc
    15901 accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt ctagcgcttt
    15961 gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag aattggtaaa
    16021 ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg tatgaaccat
    16081 gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga cattttctag
    16141 ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt tccctgtccc
    16201 tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg gcggacaaac
    16261 cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg attcgcttaa
    16321 cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca agtcaatcgc
    16381 tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat ggtaggtttt
    16441 cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta ctttctcacc
    16501 agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt ctttcacatg
    16561 ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct gcggaatatt
    16621 gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat ttcgccaagt
    16681 acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt gcatcattgc
    16741 actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga atacttctgc
    16801 tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg aataggtcca
    16861 gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag gagtaaaaga
    16921 tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta aagatgtacg
    16981 accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga tttaactccc
    17041 tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg attattgtat
    17101 acttttgtac gtacatgtca agcagttatt tacaagaata caataaaaaa cacttgtttt
    17161 gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg ccttctcgaa
    17221 attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat cgtttattta
    17281 tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta tgaccggcgc
    17341 cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga taggatggat
    17401 cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc ttctttctga
    17461 taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg ctggttctgt
    17521 cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa ggattcttct
    17581 aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg aaatggctcc
    17641 tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt ctggcatgct
    17701 cctttcctat gttgcagatt atttgctaaa agggttgcct gaaacgatgg cttggcgcgt
    17761 gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt tagctctacc
    17821 agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa gagtattgaa
    17881 ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc tgaatgtcaa
    17941 acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat atcgttcctt
    18001 agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta atgctatttt
    18061 ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg atgcattgat
    18121 gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt tggttattgc
    18181 agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg gactttcttt
    18241 catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt tgttgattct
    18301 cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac tgacatgggt
    18361 aatcgtaggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc tagcctcttc
    18421 ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga ctgcttctat
    18481 gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg ttttatttat
    18541 tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac aaagtgcatc
    18601 taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt gtgacagaag
    18661 tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg aacaatttca
    18721 aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt aggagaacac
    18781 cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa aagctctcac
    18841 aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg tctcttattt
    18901 ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa tatttggctg
    18961 tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc ccaacttttc
    19021 ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag aagaatcgtc
    19081 tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca acttatctag
    19141 cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt gttcttcttt
    19201 caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga aagtaatgat
    19261 atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac ctttcatcct
    19321 gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat gtcctttttt
    19381 tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa tataagaggc
    19441 atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt ctctcaaagc
    19501 aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa cgatcaaacc
    19561 atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt gatcgttatt
    19621 cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga ttcctcggat
    19681 aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg tttttgttct
    19741 gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg accctttttc
    19801 tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg tatgtctgct
    19861 gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta cttcgtattc
    19921 ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg ccatttgcct
    19981 tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt attcaagcaa
    20041 aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat tctggaaatc
    20101 cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg tctaacggat
    20161 cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag cacccgtctt
    20221 ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttta gaagaaacga
    20281 atactggctc cgataattta tgccatgatt cttttaataa aagattcttg tgctcatcag
    20341 cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac gtaatgaata
    20401 ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc cgtttttccc
    20461 attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa ggattatcca
    20521 tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg gcccacacat
    20581 aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt tcccttttcg
    20641 tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc tgattttcaa
    20701 gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg cttgcagcaa
    20761 agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc aattcactca
    20821 tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct aattcattca
    20881 gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct gtaaaataat
    20941 aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg ataggattat
    21001 tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa ttacattcca
    21061 agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa aatagtttat
    21121 ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat cgctactggt
    21181 taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg ttatcttttg
    21241 ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat ctacgatcac
    21301 actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc aaatgatttg
    21361 ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat aataagttgg
    21421 attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt ttactaacaa
    21481 tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc ctaatgaacc
    21541 aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac cgacttcaaa
    21601 atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg ctagatacat
    21661 tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga tttcttcaat
    21721 ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac gctcattttt
    21781 aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca aagctaaata
    21841 atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt acgatggtaa
    21901 acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta acttcatgac
    21961 ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc ccacttgcca
    22021 tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct ttttttgccc
    22081 attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat attctactaa
    22141 attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg gtttcgcttg
    22201 tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt ctatacctcg
    22261 ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat ccacccaatc
    22321 ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta attcctttac
    22381 ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc ccggttcata
    22441 aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca gccgagaact
    22501 gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt ccaacagctt
    22561 tccctacctt tcatttccta tagtcaagat aatagataca agtttccata cgccaccagc
    22621 aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta ataacaatac
    22681 gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt gttcaaaaat
    22741 ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata aaagaaacat
    22801 ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct gttcttctaa
    22861 taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca agttcttacc
    22921 aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc cctcaccttt
    22981 ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa taacaggcaa
    23041 acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa tcaactgtcc
    23101 ataaagttta gtctcaacta ttttcatctt cctcactctt tcattccaga catagctgtt
    23161 ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag cattgaaata
    23221 accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa taaagctaac
    23281 cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata aagcaagtca
    23341 ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt gctcaatggc
    23401 aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc tgcctcttcc
    23461 aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc
    23521 atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt gacttcaata
    23581 aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac gaaatagaac
    23641 aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag agaatcaaag
    23701 aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat gtaatctaat
    23761 aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc tgcaaatatt
    23821 ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat cagccacaaa
    23881 aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat gattttttta
    23941 ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg tgccatacag
    24001 gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag tggagatagc
    24061 tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc ccaattcata
    24121 aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct taaattgtgg
    24181 gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa tcagattcgt
    24241 taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa aaaactgctg
    24301 tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga ttccctgaat
    24361 agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca tgatggctac
    24421 cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga
    24481 aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa taatcgcaat
    24541 tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct
    24601 ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc
    24661 gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat cactaaatat
    24721 acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt
    24781 aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata aaactaaaaa
    24841 agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt tggtgaatcc
    24901 gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg tttaaacgaa
    24961 tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat ctgcaaatac
    25021 taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata aagttctgta
    25081 ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg tctcaggatc
    25141 ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga tcgcagagat
    25201 ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata attcagatac
    25261 aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg caggaaaacc
    25321 attttcgata ctccattttt taccaatttc agttgtattc caatatttca aaaactcata
    25381 gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta cgccatctaa
    25441 aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat tataatcaat
    25501 ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg ctagttgacc
    25561 agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag tcttgtctac
    25621 atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg caaattcaga
    25681 cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt tatataacgg
    25741 tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat cagttaattt
    25801 tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat ctaatccagc
    25861 ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca tggggacaaa
    25921 atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat cagatttatc
    25981 tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt ctacatactg
    26041 tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag aaatcgctgt
    26101 aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt tgatgttttt
    26161 atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat cagatgcagt
    26221 aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg ctgaacttcc
    26281 gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa ttaaccattt
    26341 tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc acattccaag
    26401 atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt gttgaatgac
    26461 ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat ccactcattt
    26521 cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt agattcattt
    26581 cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt acggctatac
    26641 tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg
    26701 ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa tagatcgtct
    26761 gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacattt accaattgag
    26821 caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat agacagccta
    26881 atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca atctgccaag
    26941 gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca tcaaaggaca
    27001 ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc gctgtcactt
    27061 cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga tcattttttt
    27121 ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct tgatcaagaa
    27181 ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg cataaaacca
    27241 cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct aaccgtccat
    27301 attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc cctaaacacc
    27361 atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca ctccaataag
    27421 ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact ccacgagtac
    27481 ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta taaaattcat
    27541 gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt ctaggtctat
    27601 ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt ccaccaggat
    27661 atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg cgaaatccac
    27721 ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct cgtcccatat
    27781 gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg acactactaa
    27841 tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa tacacctcgt
    27901 tgaaagcgtt g
//
LOCUS       DACCXM010000023        27855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCXM010000023 DACCXM010000000
VERSION     DACCXM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.024"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(419..1363)
                     /locus_tag="IVY61_000869"
     CDS             complement(419..1363)
                     /locus_tag="IVY61_000869"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7970001.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1659..2603)
                     /locus_tag="IVY61_000870"
     CDS             complement(1659..2603)
                     /locus_tag="IVY61_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7970002.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2824..4038
                     /locus_tag="IVY61_000871"
     CDS             2824..4038
                     /locus_tag="IVY61_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7970003.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4060..6075
                     /locus_tag="IVY61_000872"
     CDS             4060..6075
                     /locus_tag="IVY61_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP7970004.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6158..7531)
                     /locus_tag="IVY61_000873"
     CDS             complement(6158..7531)
                     /locus_tag="IVY61_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7970005.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7557..7841)
                     /locus_tag="IVY61_000874"
     CDS             complement(7557..7841)
                     /locus_tag="IVY61_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7970006.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7843..9858)
                     /locus_tag="IVY61_000875"
     CDS             complement(7843..9858)
                     /locus_tag="IVY61_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7970007.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10037..10342)
                     /locus_tag="IVY61_000876"
     CDS             complement(10037..10342)
                     /locus_tag="IVY61_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP7970008.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10431..11462)
                     /locus_tag="IVY61_000877"
     CDS             complement(10431..11462)
                     /locus_tag="IVY61_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7970009.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11669..12544
                     /locus_tag="IVY61_000878"
     CDS             11669..12544
                     /locus_tag="IVY61_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7970010.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12696..13538)
                     /locus_tag="IVY61_000879"
     CDS             complement(12696..13538)
                     /locus_tag="IVY61_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7970011.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13630..15507)
                     /locus_tag="IVY61_000880"
     CDS             complement(13630..15507)
                     /locus_tag="IVY61_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7970012.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15504..16925)
                     /locus_tag="IVY61_000881"
     CDS             complement(15504..16925)
                     /locus_tag="IVY61_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7970013.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16928..20317)
                     /locus_tag="IVY61_000882"
     CDS             complement(16928..20317)
                     /locus_tag="IVY61_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7970014.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20574..21965
                     /locus_tag="IVY61_000883"
     CDS             20574..21965
                     /locus_tag="IVY61_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7970015.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22038..22685)
                     /locus_tag="IVY61_000884"
     CDS             complement(22038..22685)
                     /locus_tag="IVY61_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7970016.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22693..23379)
                     /locus_tag="IVY61_000885"
     CDS             complement(22693..23379)
                     /locus_tag="IVY61_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970017.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23385..24458)
                     /locus_tag="IVY61_000886"
     CDS             complement(23385..24458)
                     /locus_tag="IVY61_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7970018.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24680..25354
                     /locus_tag="IVY61_000887"
     CDS             24680..25354
                     /locus_tag="IVY61_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7970019.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25359..26699
                     /locus_tag="IVY61_000888"
     CDS             25359..26699
                     /locus_tag="IVY61_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7970020.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26848..27834)
                     /locus_tag="IVY61_000889"
     CDS             complement(26848..27834)
                     /locus_tag="IVY61_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP7970021.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8123 a         5267 c         5045 g         9420 t
ORIGIN      
        1 tgataaacct ctctcgaacg gttttttatc aaaaatcgtc atttttccat caaaatctgc
       61 ttatgcaaaa ttataaaaaa attttgcagt ttttcttact ttttttgtcg tttaattgaa
      121 tgataaaaac cagtttcttt attcagttaa tcaaatatga acgaattaga tatgaatcat
      181 tgtatttgcc gttgcggaag gattttttaa ttaaccacag ataaacattg ataacgattc
      241 gcttattgta atcaacagat taaatttcat atgttcatat gtacggatac attttcggac
      301 cgattaattt taaccattct atttatttgc ttacctatta gatatcaata aaaaactgct
      361 taacagctat ttcagctgtt aagcagtttt aggattttta gttcatattc attgtttctt
      421 ataatacagt gttcaatact tctcttaaag cacttgttga tttgctgaac attgcttttt
      481 cttcttcgtt gattgataat tcaacaactt cacgaacacc attttcgtca acgattgatg
      541 gcacacctgt aaagatatct ttttcgtcgt attcaccagt taaataagca gatacaggca
      601 atacagcttg ttcgttattc aagatagctt ttacgatacg tgcagtactc ataccaatac
      661 catagtaagt tgctttttta cgatcaatga tctcataagc agcattacgg actttatcag
      721 caatcacatc taattcatct tgggcaatac gatggtcttt ttctacaatt tcaaacactg
      781 gtttaccacc gacagttgta tgtgaccatg cagcaacttc tgaatcacca tgttcaccta
      841 agatatagcc gtggacgctg cgtgggtcaa cttttaattt caatgcgatt tctttacgga
      901 aacgagttgt atccaatgtt gttccagtac cgataacacg tgatgttggc agaccagatt
      961 cgttccaagc gatatatgtc aaaatatcta ctgggtttga agcaacaacg ataataccgt
     1021 cgaatcctga tcccatgatt tcttttacga tttggcgcat gattgacgca tttgttttaa
     1081 ctaaatctaa acgagattga ccaggttttt ggttgatacc agctgtgata acaacgatat
     1141 cagcatcttt acattcttca tagccacctg accatacagc cacattttca tcgccccaag
     1201 ccataccatc taaaaggtcc aatgcttccc cttctgcttt ttcttgattc acatcgatca
     1261 atactaattc attagagatt ccttggttga tcattgcata agcgatactt gttccaacaa
     1321 aacctgtacc gacgattact actttacgac ttgttttttt catgaataaa cagctccttt
     1381 tcataactat ttgttacttt catgaaaagt atagtcttgt ttattcattt tatcaacatg
     1441 ttaattataa tagttgttat tataattttt gatggaaacg aattcctgaa tacgcataac
     1501 agataaatga caaatgttta cacctatatc ccctgataat acaaccaaaa gtttagaact
     1561 gaaaataaat ccaaaaaaat tcgctgcaac gatataataa gacagcaatc gcacctaaaa
     1621 caattttcct tcatatgctt tagtgaaaga ttttcttctt aatgtttatt tctccattca
     1681 ttgggagtac atgttgaata cttcttgaat aatttgaata aagaagtcgt ttgtttatat
     1741 ccgatacgat tagcgatttc ttccaacgaa aaactcgttt cttgaatcag ttccaacgca
     1801 aagtcgaatt ttttctttag aaccagttct tgaaaggaag catcaaaata cattttcaat
     1861 ttattactaa gatagaccgg attgtagcca aaatgagctg ctgtttcttt taacgtacaa
     1921 gaagtaaaat gcttatcgat ataatctaat actctaagca tctcattttc tgcgcatcca
     1981 tctgctgatg aaaagctttg ttgttctaat aaatgagtca attcgatcag taatagtgat
     2041 gtatagagat ttaacgaacg gatcttatga ctgtatgtat caaaataaat caacagcata
     2101 ttttcaatca aatgcgtcaa acgacttgtt tttccgccgc gaaaatgaat gaattgtgta
     2161 tgttcccctt tttcttttga tgcatttatc aaaaaagaag aaagtagatt atttgtgctt
     2221 gccaaatgat tcaatatctc tgtagataag gtttcttctt ttaataagat gttgattaaa
     2281 atatcctctt gccctgtgta ttcgattctt tgagtagctt ctttatcaat gatcaccacg
     2341 tcactttctt ccatctctat cctttctcca ttaataattt gtaaacatgt accgctatac
     2401 atataattga tttcaacaaa atcatgtgta tgctcgggaa cttccgcata tcgacggtgt
     2461 ttactgatat gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc
     2521 tgaagatggt aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt
     2581 tcgattgatt cttcgttacg caaaaaaaat gtctaattca gctagttcca attgactccc
     2641 tcctttttgt aagcggtttc tttcgcttat tagttcacgc tgtactgcta ttatatgcaa
     2701 agctattttt ggtttcataa agactttcta tggttgggac atgaacttcc tgtgattccg
     2761 ctattgagag aataaccact tcatactata tttttgataa gcgcttacaa aaatgaaagg
     2821 aacatgctta tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc tcttattttg
     2881 acttctgctg gtgcagtcac gtctgcaatt cccagtatgt cagctactta tgccacgcgt
     2941 tctttatctg ctgttgaatt attaacgact gctccctctt ttatggtcac tatttttgtt
     3001 ttgattagta gttttattgc taggaaaatc ggacaaaaac aaactgtgat catcggatta
     3061 gtgattgcat cgatttgtgg tatcctcccc ctcttctcac aaagttacag tattgtctta
     3121 gcttcaagag ctggactagg tattggcttt ggattgatca attctttggc agtcagcatg
     3181 attgctgcct tttatcacgg agatgaacga gcctctatga ttggttttca aagtgcattt
     3241 caaggatttg gtgcagcttt aatgacattt gttgcaggac agctagtaca gttcggttgg
     3301 caatatacct ttttagtcta cgccatcagc ttacctattc ttgtcttgtt tgttttgttt
     3361 gttcctacac ctgaaacaac tgaacttcaa tcgcatggca cagccccaac aagacaagta
     3421 atcaaaacga atcctaaaat gattagttat ggtatttttc tcttgctggt catcgtggta
     3481 tataatgctg tatcgattaa attagccaca gtcctgacga cttattctct aggaaacgaa
     3541 actaatgcag cgaatatctt aacaatcatg caattggcaa gtatggctgc cggagtttgt
     3601 tttggctggc ttcagacaaa agcacatcgc tatatgctta cactttcttt actactgatg
     3661 ggaatcggct ttgtattgat tgccactgtg ataaacctgt atgtgattgg gtttggtgcg
     3721 atcctaacag gaatttcttt ttcgttattc attccttact tattcaacca agtaagtatc
     3781 caagcgccca agtacgcgga gagtttcaat acttcaatct tattggttgg cgctaattta
     3841 ggtactttca ttgcaccata tggtttgctc tttctctctt ttgcttctct tgggagtaaa
     3901 acacttgctg ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct tattagcctt
     3961 ctagtagtac acaggacgaa tagaaagaca gtgataagag tagatgaaga aataaaagaa
     4021 caattgacaa caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa
     4081 gatagatggc tttggtatcc tggagacttt gagatccgcc atggactgct acaaaatttc
     4141 caacgggaag aacgaggttt tgactggcca gcgtactggt acatggatga ttgccatcgg
     4201 aacgtcaagt ttaagagata ttactttctt gatcaacctt ccatgtttaa agtgacgatc
     4261 caaggagttg ggtatgtaga aattaatgga caaaaacatc catgtggaaa atggctgact
     4321 tgtccagctg gtaaagcaaa aattcgtatc tttgtaggac atacatcagg gctaccagcg
     4381 atgtttatcg agggagacga agtaaaatca gatattggct ggacagcaag taattttatt
     4441 gaagaatatc cagctggctg gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa
     4501 atctattatc aaaaagaatg cgtccatcca gtcactgaaa cggagattgg cggaggaatc
     4561 ttattcgatt ttgggcgagc agtgaatggc tgtgtagaac taatatctct ggatgaccat
     4621 atcgaatcta tcactctatg ttatggagag tccgatgtag aagctatgga tatcgattat
     4681 tgctactata agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc
     4741 tatttgttta taccagaggt ttctactgga atggttcaac ttcaagtttt tcatgaattt
     4801 ttatctcttt cgaaacaagc tgctttttca agcgattctt ctttactgaa tcagatctgg
     4861 tcagtatcag aagaagcctt caagttatgt agtgatttgt tttttatcga cggtatcaaa
     4921 cgagatcgct ggatttggtc tggagatgcg tatcaatcat tcatgatcaa tcaatatctt
     4981 ttttttgacg aggaaatcaa taaacgaacg atattagcat taagaggaca aaatgaaatc
     5041 aagcagcata tgaatactat cgttgactat tcgatcttgt ggttgattgg tatcgaaaac
     5101 cactatatga tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa gatgcaatca
     5161 ttaatgagat acttgatgga gcaaacgaat gaattagggt ttatttatgg gagagcaaaa
     5221 gattggatat ttattgattg gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa
     5281 gtcttattac tgaaagccta tcattccatg atcacttgcg gagatatctt agctgaaaca
     5341 tatggagaga acgaaaatta tcatactcat ctctatcaag aaaaatatca actattgaaa
     5401 caaaacctat ttgactactt ttgggatgaa gaacttggcg cttttatcga ttgctatgaa
     5461 tctgggagaa gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt cgattttgct
     5521 aacaaagacc aacagcaatc gatcttaaag aatgtcttat tgaatgacac tatcacacag
     5581 attactactc catatttcaa attctatgaa caagatgctt tatgcaaact cggacagacg
     5641 aatatcgtct accaaacgat tcttgattat tggggaggga tgttatctca tgatgcggta
     5701 actttctggg aagaatacga ccctactcaa acaggaaatg aaaagtacag tatgtatgga
     5761 gatccttatg gaaaaagctt atgccatgca tggggagcaa gcccaatcta tcttttagga
     5821 cgctatttta tcggtttgta tccaacaaag ccaggttatg aagaatttat gatacaacct
     5881 aatctagatt catttgaaac actgaattgt acgctaccta tcaaagatgg aaagatttcg
     5941 cttgaaaaaa caaaagataa gatcgtgatt acttctgata gaacaggcgg aacagtgaaa
     6001 atgaatggta aaagttatcc tcttctagca aaacagccac ttgtcttaac aagctattca
     6061 aaaataaatg cataaaacaa aaaactgctt gcgacatatc agaagacttt aaatcttctt
     6121 ctgatattgt ccaagcaatt tttattttga agtttgctta attctgttct ttagaaacaa
     6181 tgtttgttct ttttccggca aaaagaaagc tagataatac gacaagtaaa aagaaagcga
     6241 tgattccaac tgtcaaggct atttgaccgt attctcctag tttcccaagt acaagtcctg
     6301 gtactagata atcagtatcg gcaaatgtag aagaagcgat tttaagatct cctaatactg
     6361 gaaggataaa taatggcagc caactaatca ataagctatt gaaagcagaa cctaataccg
     6421 cccctcttct accgccagtc gagttgccga agacaccaga agcagctccg cagaagaaat
     6481 gtcctactac cccaggaatg ataactgtag tttgcaagcc tatcatagca aacatggata
     6541 gtgttcctac aaagaagcta acaaaaaagc caattaatac agcattaggt gcatatggaa
     6601 aaacgatagg aacatctaat gctggtttag aatcgggtac gagtttttta gcaatccctt
     6661 ggaatgctgg aacgatttct gccaacacca ttcgaacccc ttgaaggacg ataacaaacc
     6721 cagctgtgaa tgttccagct tggatcagtg caaagattac agcgttgtct ccattgctta
     6781 gttctctttc aacaaatcca ggaccagcta acaaagcaag tgcgacatat acgatcgaca
     6841 tcaagagcat gatcgataca gtagaatctc gcaaaaagct gaaactttta ggaatcttta
     6901 tgtcctctgt agaacggtct ttgtttccaa aaaactttcc gatccatcca ctagaagcat
     6961 aaccgatatt tccggtatgc cctaacgcaa cttgattgtt tccagtgatg ttcttcataa
     7021 acggttgaca aagagcaggc gtaatagtta atagtgttcc ttcgaagacc ccgcctaata
     7081 agatcatcca caaatttgca gtaaaccccg cgcttactaa aatgactgct gttagacaag
     7141 aaacataaag cattgcttga cctgtaagaa aaatgaattt atatcttgtg aatctagcaa
     7201 gtaaaatatt cacgatcatc cctactagca tgatcaaagc agtaggtgta ccgtattgtt
     7261 gcaaagcaat cgccactact gcttcattgc taggaactac tccttgagta tgcagagctg
     7321 tttggaacat cgaagcaaaa ggggttagtg aattactgat taaaccagca ccgccgatca
     7381 gtactaggaa tccgacaaat gttttgatag ttccttggat cacttctgaa aatttcttct
     7441 tctgaagaac gagaccaagc agtgcaatca aagaaacgag gatggagggc tgactgacga
     7501 ttgacacaaa caaattcaac aatttatcca ttgatagttc ctcccttgaa tcatttttat
     7561 tcttttgaca agatcttcgt tactttttct tttacttctt cgctgtcaat gatcgattgc
     7621 aaaggtacga gcttttcatc aggtatagaa gttagcgtat ctttcaaaga atgctcaatg
     7681 aagaaataat cagccatatc gctagataca cttccaacat ccgtatgact tacttcaaac
     7741 tcgtccacat ctgctccaag atctgcaagt gcttgtttga tgttcatctc taccataaaa
     7801 cttgttccta atccactttg acatactgca aaaattttca tatcattcgt ctccttttct
     7861 tatgatcgct aatatttctt cttttgttga tgcgtgtaat aaatcttgaa gattttgttt
     7921 attagaaagt agctttgtta aatttgccaa agcccgaaga tgtgcctcat tatcgatagc
     7981 agctaaacag atgaacaaac taatgggatg ggctggatta tcaagcagat ccacaggttg
     8041 ttgaagtttc agcaaagaca tccctacttc gtttacgcca tcttctggac gagcatgagg
     8101 taatgcgatg tgatcgccaa tatggataaa cgcaccatac tgctccacgc gctcgatcat
     8161 agctttgata taactttctt ctattttatt ttctgataat agcggctgtg cggcaaatgt
     8221 gattgcttct tgccagtttt ttggttgatc cgataattgg atcatttctt ctgttaataa
     8281 ttcagatagc ataggacgtt gatcctcctt cttttctaaa agtttgttca ttttacgatt
     8341 taatacttga tagagctttt cttcgtctac gccttctttt aattcgatat aaggcagtag
     8401 cgccgacaac aattcttggg cagaaggcat tgaaaatcct ggtatcagcc aatctctttg
     8461 tacttgattg atcagctgat ttttttccaa gtcagtcatg attggatgca gaacatagag
     8521 agactttttc ttatcagtaa gtgtgagagg tacagtagaa aaaataatat cgtaagaagt
     8581 ttcgtcaacc tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga tcatcgggaa
     8641 taattgctga agttcacttt ttaagatcaa tgaagagcta atgccacttg gacagacgat
     8701 cacggctctt atttttctat ttctttcttc tgcgtccgct tttctgatct ctccgccaaa
     8761 tagtatagtg aaaaaaccaa tctcttcttc agggatgctc ttccctattc gtctttctaa
     8821 cggaagtaat gcttttcttg tcatttcgta gatggaagca tattgatgct taatttgatc
     8881 gatcatcaca ttaggtaaat aaaaagaata ggaaatacga aaataggcag ggaccaagtg
     8941 ataaaagaca ttcatcaata attcacgaaa cgaagcaaat tctacggcca tcaggaattc
     9001 catgcgatgg atgacttctg aagaacattc cagcaaaaaa tcaagtgcag gctcctggac
     9061 atctccttca acgatcgatg ctaagcaaag agagaaaaat ataatctccg aattttgatg
     9121 ttcggaatag ccaaggccag tcaatacttg tcgactaatg tcagtgactg caagagaagc
     9181 caacagttca gcatctttta ccagttccat ggatgttttt ccaattcgaa tggttgtcaa
     9241 actcaaataa taagctaatt catccattcg atttggtgcg aatcgaagat ctgatagtga
     9301 aatcgtttta tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt
     9361 taaatacgtg agcccaaatc ttccagcaag tgactgcatc aaacgagcag tgcgattttt
     9421 tgcttctctt cttaatattg cttctgaccc tagcagccta aaaccagttt tcctagtata
     9481 cgacagctga caatcttcca cttctaattc atttcttatc tttttgatat ctgacaggac
     9541 tgtcccttta gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt
     9601 cgaataaacc atcaaataaa gaagatctct tcgctcttgt tcggtataca cagcttcaaa
     9661 tcgagtaata tccaaaaatt tttctgccca gtcgattgct cggaatttct ttatacggat
     9721 acgggtatca tccacatatt cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt
     9781 tacttcgatg atctctgctg ctttttctag actgatttct cttggtgttt tcaggagata
     9841 actaattttg tagttcacag cgctgttcac ctcctactta tattctagta tggtagcgct
     9901 tttaaatcga ggggtgtttg gtacaactta cagttggatt ttttttacga ctgtaccgaa
     9961 cgatttctat cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg
    10021 aattgatttc gtccgatcaa tgatgatcat gtttattttt tgaggtcagt tcttcaaatt
    10081 tttctttgac atcttcaacc gctttttcga tatctttttc aaagtgattg ggctcattag
    10141 gctttgtttc ctcttctaca gaatttgaac cagataaagc tgtttcttta ttttctgttt
    10201 caccagagtt tgtgccagct aatgattctt ctttgtcttc tgtttcacca gaattagttc
    10261 ctgctaaagc cacttcttct tctgttttag gggccattga acctcttaat cgtttgtgtt
    10321 tttcaccgtc ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta
    10381 caataagaca gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt
    10441 ccttctgatt cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt
    10501 tcttcatctt tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag
    10561 acacggctgt aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca
    10621 tctatatagg tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca
    10681 ggcatgtaaa acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc
    10741 cctttttttc cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt
    10801 atcgaaattt tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct
    10861 ttccctaaaa gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga
    10921 acttgatcta ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca
    10981 tgcgtatttt ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata
    11041 tcccctaatt ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt
    11101 ctgttttggt aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct
    11161 gcttcctttg acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt
    11221 gccatttttc catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc
    11281 tcaggatcat cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc
    11341 cacactggtt cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt
    11401 gaaaaaggca aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc
    11461 atgagtggtg cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat
    11521 ttataatagc tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg
    11581 aaattaatgt tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat
    11641 cagtaagata gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga
    11701 gttgtttacc acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc
    11761 agtctacatg acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat
    11821 tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg
    11881 ggaagttcat caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc
    11941 aagtgaagat agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat
    12001 aaaaaaagca caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa
    12061 caaggcacag cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt
    12121 ccagaataaa atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc
    12181 ttcttggaat gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg
    12241 tcgcatccat cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt
    12301 gctgattacc ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat
    12361 atcgcaggag gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga
    12421 ttacgtatta aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg
    12481 caacttgaaa aacaaacaag aattgatgca aaaaggcctt cttaaagaat atcggaaaac
    12541 ttaagaaaag atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata
    12601 tcatcataaa aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac
    12661 gattcgaata gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac
    12721 gattgacgaa aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca
    12781 tacaggaaaa atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca
    12841 tgataaggaa cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta
    12901 caagttagga gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc
    12961 ggttctaccg tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata
    13021 tagaaagaat cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt
    13081 cctcgatggc tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct
    13141 tccaataatg aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa
    13201 tggacattga tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta
    13261 tcctttttcg caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct
    13321 tttgctaatt gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca
    13381 ttttctttga ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc
    13441 agcgcgtcgc taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc
    13501 atcagcagat ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact
    13561 gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt
    13621 gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag
    13681 aaggactact cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt
    13741 acctttgtgt ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga
    13801 ttgttcatcg atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt
    13861 cgtttcttcc agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact
    13921 tgtggttgta aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga
    13981 tgggtcgatc gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc
    14041 tccagcaagt gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc
    14101 agtaacgaca tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc
    14161 ttggttagaa aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa
    14221 tttcaatgta ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa
    14281 tccgtctgtt tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata
    14341 taaggcataa gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt
    14401 atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa
    14461 tgggatattg acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt
    14521 caattgaggt gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg
    14581 tgtttggcta tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc
    14641 attatcagga gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat
    14701 caactggcct ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg
    14761 accagtaatg aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt
    14821 gacactttgg atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc
    14881 tgctccattt agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc
    14941 attaccgact gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt
    15001 ataagagcca tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc
    15061 ggcagctgac actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt
    15121 ttgtttttta ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac
    15181 tggtgtacca ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt
    15241 atctcgttgc gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa
    15301 tccttggtat tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg
    15361 taaatcagtc attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac
    15421 tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat
    15481 aacgtttatt tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac
    15541 cagattgcaa agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg
    15601 tttgtttttg gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg
    15661 tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt
    15721 aaaatataac cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg
    15781 gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt
    15841 gtccacttgt atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg
    15901 aatactgcgc cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta
    15961 ctccctgtat atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg
    16021 ttgttgttga tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg
    16081 gtcagttttt ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg
    16141 tgaggtttct tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt
    16201 aaactattag gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga
    16261 tagtcaagga tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc
    16321 gtaaaatcat ttctaggctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt
    16381 tcttcatttt taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca
    16441 ggaagaacaa ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca
    16501 atgaaaagat acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg
    16561 ataccatctt cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac
    16621 gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga
    16681 tggtaaaaag aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac
    16741 gtttgtaata aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt
    16801 gggaaaagca atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc
    16861 tgagtaaaag ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt
    16921 ttcatattta gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac
    16981 aaccaatcat ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg
    17041 gtacttttgc ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca
    17101 gaacagattc atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag
    17161 taactggctc ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag
    17221 ttccaggttt taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt
    17281 ctaagtccat ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt
    17341 cttctttttt gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc
    17401 atacattatt tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga
    17461 tttggaattc ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga
    17521 ctcgatattt accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt
    17581 ggctgtcaat tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc
    17641 ctttaagatt tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa
    17701 cttcaacaaa aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt
    17761 ttccatcaat ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc
    17821 ttaatccatg cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca
    17881 atttgacatt ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa
    17941 tagaatctcc tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat
    18001 ttttcgttat tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat
    18061 ctatttgttg agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg
    18121 cttgaccttg attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat
    18181 ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt
    18241 taatgattcg aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa
    18301 tctcaaaagt aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact
    18361 ttttgatgtg aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt
    18421 cattggtatc aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg
    18481 gatggaagtc ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt
    18541 cttttcctaa atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag
    18601 tgaccgttgt aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga
    18661 atggttctgc gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag
    18721 cttttttggc taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat
    18781 cgcctttttc atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga
    18841 gaccagctgc cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt
    18901 ctttgagtgc catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata
    18961 gattgttttg gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat
    19021 cttgcgtatt agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct
    19081 gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc
    19141 catttggaac ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac
    19201 catttgtccg tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca
    19261 tctgtactgc accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat
    19321 tgattttgtc tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct
    19381 ttacttcacc gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga
    19441 gatcaagagg cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca
    19501 atccgggtgt acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg
    19561 ttcctccata ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta
    19621 taccatccca ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc
    19681 ctgtaggttg ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag
    19741 gaatcgtttg attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg
    19801 ggctgacaga attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta
    19861 ctgacacatc tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat
    19921 cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat
    19981 cgatagtttg tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat
    20041 aagcatagcc atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa
    20101 tttctttttc tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt
    20161 tttttccaga ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat
    20221 ctgtagtgtc tgctattgca gataatggca caaagtttga taaactcaat attatcagca
    20281 tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt
    20341 tctgttttga cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt
    20401 attttaatat gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac
    20461 caattggttt catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa
    20521 gggttgaggg agaaaaacat atgatcaggt tgttggaatt aaactataga aagatggaaa
    20581 aaatcattcg aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac
    20641 gaattggtag ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg
    20701 gaacggatgt aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg
    20761 taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat
    20821 taaatgagat tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca
    20881 tcagccggtc ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt
    20941 tattaaaagt gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt
    21001 tgcgtatctt tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc
    21061 caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt
    21121 ttcaggaaga aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca
    21181 tgagagaatc ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga
    21241 taaatatcga taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca
    21301 gcagctatat aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga
    21361 aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat
    21421 ccgctgattg caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt
    21481 ttttctcccg tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg
    21541 cacagcagat gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat
    21601 tttattccac tctactgaat cagaatctga tttattattt atctagctat atcttttgga
    21661 tgcttatcgg taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca
    21721 gcgacttaag tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg
    21781 tgcataaaat tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc
    21841 ttctcgagta tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata
    21901 tattgattga tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt
    21961 tatgaataat caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata
    22021 cacatagctg tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag
    22081 tcttttaaca actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt
    22141 acgagggtca tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa
    22201 tgattgattc ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct
    22261 ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag
    22321 atactttcgt gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa
    22381 tgttgtttgt tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt
    22441 ttgatcgtat acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc
    22501 gacatcccca tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa
    22561 aatatctcca gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa
    22621 gtttcttttg gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt
    22681 gtcatgaagg attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg
    22741 ataaacgcga tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt
    22801 ttgtttctca tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc
    22861 caaactagct gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc
    22921 aattgccact ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga
    22981 ctggatatct aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt
    23041 aacttggtca actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct
    23101 ttggagaata aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc
    23161 agttagtgag tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga
    23221 aatggtaagg aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc
    23281 ttcctttaca taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa
    23341 tgttttggta acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct
    23401 gtaattgggt ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca
    23461 atgatcaaaa tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg
    23521 cttggtaaaa tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc
    23581 ccaacaataa aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt
    23641 gctttcatta ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg
    23701 accacggcag caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg
    23761 gcagaatagc caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc
    23821 gatgcttttt ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt
    23881 tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac
    23941 gcatagatga cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa
    24001 ggatcgtcgt aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga
    24061 ttttgagaga tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt
    24121 aataaaaatg cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg
    24181 attgctccgc tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct
    24241 cctcttgtta attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct
    24301 tgtgcgtccc aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct
    24361 gacaacatga atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt
    24421 aatttagaat agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag
    24481 attgatagtg acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac
    24541 ttgtgttgtt tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca
    24601 tgagtcataa aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc
    24661 aaatcaatga ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc
    24721 caataatata aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag
    24781 tgaaggactg ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat
    24841 gccggaaatg aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc
    24901 agtattgatt ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg
    24961 tgcagatgat tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc
    25021 attattgaaa cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag
    25081 ttgtgattta aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa
    25141 agagtttgaa ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca
    25201 gatatttgac cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta
    25261 tatgagcaac ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt
    25321 acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa
    25381 agcaacgtag tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt
    25441 tgggattcat tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat
    25501 tagagaaaat aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag
    25561 gcgatccctt tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat
    25621 taatggaacc atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg
    25681 agatattgaa taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg
    25741 atacgaaaaa tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg
    25801 ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca
    25861 tccaaatttt agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta
    25921 tgattttgtg tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta
    25981 gtctaagcat gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg
    26041 cttctcatga attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta
    26101 ccaagcctaa tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga
    26161 cgaggcgcct cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc
    26221 agcagctgct ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac
    26281 catttcaaga gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat
    26341 tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg
    26401 cattaaaata tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag
    26461 aatggctgat tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga
    26521 tttttgatcg attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc
    26581 ttggcttggc aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca
    26641 attatcttcc acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat
    26701 gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat
    26761 aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac
    26821 ttctgtcaca acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac
    26881 atcaccgctt gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac
    26941 atcttcatgt tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac
    27001 atctacttgc tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt
    27061 tccctgtgaa gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac
    27121 cggtacttct accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt
    27181 tgtcacatca tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc
    27241 tagatttggt ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc
    27301 aggtacgtcc atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa
    27361 gcctgtcgtg ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc
    27421 cattgtgctt gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg
    27481 ctgacgatag ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg
    27541 atataaagga tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt
    27601 atcttttatt ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt
    27661 ttcaacatct ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt
    27721 ataaataacg atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa
    27781 tgcttcttcc ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat
    27841 tcctcctagc aaata
//
LOCUS       DACCXM010000024        26919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCXM010000024 DACCXM010000000
VERSION     DACCXM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.026"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            86..1090
                     /locus_tag="IVY61_000890"
     CDS             86..1090
                     /locus_tag="IVY61_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970022.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1091..2035
                     /locus_tag="IVY61_000891"
     CDS             1091..2035
                     /locus_tag="IVY61_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970023.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2035..2997
                     /locus_tag="IVY61_000892"
     CDS             2035..2997
                     /locus_tag="IVY61_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7970024.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3024..3938
                     /locus_tag="IVY61_000893"
     CDS             3024..3938
                     /locus_tag="IVY61_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7970025.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3964..5745
                     /locus_tag="IVY61_000894"
     CDS             3964..5745
                     /locus_tag="IVY61_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7970026.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6093..6779
                     /locus_tag="IVY61_000895"
     CDS             6093..6779
                     /locus_tag="IVY61_000895"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7970027.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6799..10380
                     /gene="smc"
                     /locus_tag="IVY61_000896"
     CDS             6799..10380
                     /gene="smc"
                     /locus_tag="IVY61_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP7970028.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10389..11198
                     /locus_tag="IVY61_000897"
     CDS             10389..11198
                     /locus_tag="IVY61_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7970029.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11209..12207
                     /gene="ftsY"
                     /locus_tag="IVY61_000898"
     CDS             11209..12207
                     /gene="ftsY"
                     /locus_tag="IVY61_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP7970030.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12394..14661
                     /gene="gshAB"
                     /locus_tag="IVY61_000899"
     CDS             12394..14661
                     /gene="gshAB"
                     /locus_tag="IVY61_000899"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP7970031.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14750..15202)
                     /locus_tag="IVY61_000900"
     CDS             complement(14750..15202)
                     /locus_tag="IVY61_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP7970032.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15352..16302
                     /locus_tag="IVY61_000901"
     CDS             15352..16302
                     /locus_tag="IVY61_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7970033.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16295..17254
                     /locus_tag="IVY61_000902"
     CDS             16295..17254
                     /locus_tag="IVY61_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7970034.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17251..18006
                     /locus_tag="IVY61_000903"
     CDS             17251..18006
                     /locus_tag="IVY61_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7970035.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18029..18982
                     /locus_tag="IVY61_000904"
     CDS             18029..18982
                     /locus_tag="IVY61_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7970036.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19626..19922)
                     /locus_tag="IVY61_000905"
     CDS             complement(19626..19922)
                     /locus_tag="IVY61_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP7970037.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20277..20633
                     /locus_tag="IVY61_000906"
     CDS             20277..20633
                     /locus_tag="IVY61_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7970038.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20643..21542
                     /locus_tag="IVY61_000907"
     CDS             20643..21542
                     /locus_tag="IVY61_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP7970039.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22059..22790
                     /locus_tag="IVY61_000908"
     CDS             22059..22790
                     /locus_tag="IVY61_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7970040.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22935..24962
                     /locus_tag="IVY61_000909"
     CDS             22935..24962
                     /locus_tag="IVY61_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP7970041.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25070..26167
                     /locus_tag="IVY61_000910"
     CDS             25070..26167
                     /locus_tag="IVY61_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7970042.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26474..>26919
                     /locus_tag="IVY61_000911"
     CDS             26474..>26919
                     /locus_tag="IVY61_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7970043.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT         9021 a         4663 c         5685 g         7550 t
ORIGIN      
        1 cctttgttat aaaacgcttt attctgtgta atgtcctttt ttattaagaa aaacttccat
       61 ttaggaagca gagaggggta ttcgattgtc cgataaccaa ttattagaag ttcagaacct
      121 acatacagga ttccgactca aagatgacta ttacgatgca gtagatgacg tttcatttac
      181 tttagacaaa aacgagattt tagcaatcgt tggggaatct gggtgcggga aaagtacctt
      241 ggctactacg attatgggat tacttgatcc taataacaca aaaattacag gtgagatttt
      301 atataacgat ctgaatttaa tcgacctgaa cgaaacgctc tataataaaa tccgcggaaa
      361 tgatatcgga atgattttcc aagatccgtt gtctgcatta aatcctctca tgagaatcga
      421 agaccagatc aaagaaggac tttcctatca cacaaagatg aatgcagaac agcgtcaagc
      481 tcgtgcacta gaattgctag aacaagtagg gattcctaat cctgcgagag ttggacgcca
      541 atatccccat gaactatcag gcggtatgag acaacgtgtc atcatcgcaa tcgcaatcgc
      601 ctgcaaacct ccaatcgtca ttgcagatga accgacgaca gccctagatg taaccatcca
      661 agcacagatt cttgatttac tgaaagacct tcaggaagaa acaaaaacag ggatcatctt
      721 gatcactcat gacttaggag tcgtagcaga aatggctgat cgagtggcag tgatgtatgg
      781 cggacagttc gttgaagtag caggtgtaaa agaactgttt gatcatccta agcatcctta
      841 tacacgctct ttactgaact ctataccaca agaaggaaat catgatgcag aactccatgt
      901 gatcgaaggt gtcgttcctt ctctgaaaaa catgcctcgt aaaggttgtc ggtttgcacc
      961 acgtattcca tggataccag caagcgatca tgaagaagag cctatccttc atgaagtcgc
     1021 accgaaccac ttggtacgtt gctcctgcta taagcatttc cactttagag acgaaaaagg
     1081 agaggtgtag atgacagaat taatcacaat caaagacctg aaagttcatt accctattcg
     1141 cagtggattc ttcaatcgag taacggatca tgtatatgct gtagatggtg tggattttat
     1201 tatcgaaaaa ggaaagacat atggtttagt gggagagtct ggctctggaa aatctactac
     1261 gggaaaagcc gttgtgggat tagaaaaggt aacagccgga caaatcatgt atgaaggaaa
     1321 agatgtaacg aagcgaagca accgcaaaaa aatgggttat aacaaggatg tgcagatgat
     1381 cttccaagat tcaatgtcca gtttgaatcc taaaaagcga gtgttggata tcattgctga
     1441 acccatccga aattttgaac ggttaagcga tcaagaagaa aagaaaaagg taaaaggact
     1501 gctagatatc gttgggatgc cggaagatgc gctatataaa tatcctcatg aattttcagg
     1561 cggacaacgt caacgtctag gagtagcccg tgctgttgct accaatccaa aactgatcgt
     1621 agccgatgaa cctgtatctg ctctggatct atctgttcaa gcgcaagtat tgaatttcat
     1681 gaaacgaatc cagcaagaat acggcctcag ctatttattc atttcacatg atttaggtgt
     1741 agtaaaacac atgtgcgaca atatcgcaat tatgtacaaa ggtcgatttg tagaaatcgg
     1801 tacaagagaa gacatttata atgacccgcg acatatctat acgaaacgtc tgctttcagc
     1861 tattccgcaa atcgatgtag aaaataggga aagccacaaa gaaaatcgac gaagagtaga
     1921 acaagagtat atccaaaacc aaaaagaata ctatgatgcg actggtcgag tctacgattt
     1981 gcgtacgatc acaactacac acaaagtggc gttgaaagat ggaggtgcaa gctaatgtgg
     2041 aaaacgattc tgcgacgtgt tttattgatg atcccgcaag ttattatttt aagtgttctg
     2101 atcttcttat tagcccaagc gatgccgggg gatccattta ccggtttgat caatccaaat
     2161 caggatccgg cagtgatcga acagatgcga caagcagctg gcttgaatga cccatggtat
     2221 gtccagtact tccgttggat cggaaatgct ttgcaaggcg attttggtca aagtttctta
     2281 tacaaacgct ctgttgctga cttgatcggc gaacggatca tcaacacttt atatctatcg
     2341 atcttgacag ttgtccttac ttatttgatt gctgtgcctc tggggatgct tgctggccgg
     2401 tatcaaaatt caatcttgga taagattgtc gtaatctaca acttctttag ttttgctgtt
     2461 cctttattta tcttcggctt gatcatgcta tttgtttttg gttatcgttt aggctggttc
     2521 ccaacaagtg gatcacaaac actaggtttg caaggtggat ttatcactca atggatagac
     2581 cgtttgaaat atattctgtt accatcaatt acccaagcat ttttggcgac tgccgtcaca
     2641 atccaatatc tgcgaagtga agtaatcgat tcgaaatcgc tcgattttgt cagaacagcc
     2701 cgttccaaag gggtaccgac gaataaagta ttcagtcgac atattttccg aaatgcagcg
     2761 ttgccgatgg cttctcaaat ggggtatgaa atcacttcgt tgattgctgg ttctgtagtt
     2821 atcgaaaaga tttttggtta tccgggagtc ggaaaactat ttattgattc catcggccaa
     2881 cgggactata ccgtgatcac atccttagta ttgattttag gaatcgcgac gctagtgggg
     2941 acattacttt cagatatcat catgagtatc gttgacccgc gtattcggat tcaataaaac
     3001 caatcagaaa ggagaatgga tcgatgagtg aaaaaaacaa tacagcagtt caagaaagta
     3061 ttcctcctat ggggatacga atgattgctc gagaatttaa aaaagataaa attgctattt
     3121 tttcattagg tttgctagtg gttttattat tagtgatctt tatcggtgcg atgctgaccg
     3181 atcaagacaa agtgatgtat gttagtatct tcgacaaata cgctgcacca ggaacagttt
     3241 cgacacaagg acaaaagttc attctgggag cagatgaagg cggacgtgat gtattaggac
     3301 aattgatcat aggtgctaga aactctgtta cgatcggatt tgcgatcacg atccttactt
     3361 cgatcatcgg ggttggatta gggatcatct ctggctacta cggagggatc attgacaaca
     3421 tcttgatgcg gatcgttgat tttatcatga ttctaccgat catgctgatc attatcgtct
     3481 tcgtatcgat tatttccaga tacaatgtct ggtcctttat attcatcatg agtgcatttt
     3541 attgggtagc taaagcccga ttattccgaa gcaagacatt atctgaagca agaagagatt
     3601 atgtcagtgc ctcaaaaaca atgggaacca atgattttaa aatcatgttc cgcgaaatca
     3661 tgccgaatct aagttcatta atcattacta atttgacgat gaactttgcg gcaaacatcg
     3721 gtatcgagac aactttgact ttcttaggtt tcggcttgcc ggcaaatgta cctagtttag
     3781 gtacattgat cggttatgca agtaacggag aagtcttagt gaatcggcaa tggatctggt
     3841 tacccgcatc aatccttatt ttagttttga tgttgagtat caattatgtt ggacaagcat
     3901 tcaagcgttc tgcagatgcg cgccaacggt taggctagta tagaagcgaa gggggaaata
     3961 ggaatgaaga aaacgatttt tggatttgca gcgctgatgt ctgctgtcgt tcttgcagca
     4021 tgcgggggcg gaggtacaga tacctctgga aacaactctg gaggcaacgg caataacaaa
     4081 gaggaattaa cttttccaat tgcaacaaaa aataatgatg atgcaatcga agacggcgaa
     4141 ttagaagctg cggtcgcaac ggacagtcag tttaaaggat tgttccagtg ggagttttac
     4201 caagattcat atgatgcagc ttttatggct ccttctcatg aaccgctatt tgcaaacgat
     4261 gacgatttca gaatcacaga cgacggagct gcaacgcttg atctagatga agatacgaaa
     4321 aaagcgacaa tcaaagtaaa agacaacgtc aaatggtcag atggtgaaga tgtaacggcg
     4381 gacgatgtga ttttccctta cgaaatcatt gggaataaag attacactgg tatccgttat
     4441 gacgacactt tccgtaacat cgtaggtatg gaagaatatc atgatggaag tgccgacaca
     4501 atctccggaa tcaaaaaaat agacgacaaa acagttgaaa tcgaatataa agaagtaaac
     4561 ccaagcatgc ttcaatcggg tggtggtatc tggtcatatg cagcaccaaa acatacgtta
     4621 gaaggtgtag caatcaaaga tatggaatca agcgatcaag tacgtaaaaa tccagtgact
     4681 tttggccctt attacatgag taatatcgtt gcaggagaat cagtagaata cttgccaaac
     4741 gagcactact ggaatggtac gcctaaattg aaaaagatca cgatgaaatc tgtaccgatg
     4801 gcaagtgcaa ctgaagcatt gaactctaaa ttatatgata tgattttcca aatgccaaca
     4861 gatacttacg atacctataa agaaattgct ggctatacaa acttaggccg tgaacaaact
     4921 tcttatactt atctaggatt caagttaggt aaatggaatg cagatacaaa gagtgtggaa
     4981 tacaatccag attcaaaaat ggctgataaa tcattacgtc aagcaatggg ctatgcatta
     5041 gataataatg cagtaggtga acgtttctat gcaggtttga gaagcaatgc gacttcattg
     5101 attcctccag tattccagtc ataccatgat gatgagatga aaggattcac acaagatcta
     5161 gacaaagcga aaaaactatt agatgatgct ggctacaaag atgtggatga cgatggttta
     5221 agagaagaca aagatggaaa taaactaaca atcaactttg cttcgatgtc cggtggggaa
     5281 acagcgcaac cattggcaga ttactatatc caacaatgga aagaaattgg tttggatgtc
     5341 caatacacaa caggtcgtct gatcgaattc aattctttct atgataaatt agaaaacgat
     5401 gatcctgaaa ttgatgttta ccaagctgct tggaatacag gaactgatcc aaatccaagt
     5461 ggactatggg gaccaaatgc tgcgttcaac tacactcgtt atgaatcaga agaaaataca
     5521 aaattgatca acgatatcag ttcaagcaaa gcatttgatg aagagtacca aaaagaagca
     5581 tatcaaaaat ggcaacaata tgcatttgaa gaagcatttg cgattccaac gttgttccgt
     5641 aatgaagtat tgccagtcaa cgaccgtgtc aaagactggg attggtctta tgatgtacca
     5701 aacccatggg caaatgtttc tgttacagca gattcacgtt cataatcact aaaaatgacc
     5761 gaaaaagggc gatgtccttt ttcggttttt tgatattttt ctgtttattt ctctaatatt
     5821 caggtcaagg cgctgatttc atgacttctc caataaacgg tgttcaaagt ccagtttctc
     5881 ggtattaagt caaatttatg caaaaatatg gaaagccatt ttcacaaaat tttttcttca
     5941 tactcgaaac taaacgaatt tttcacaact tctcgtcaat cactaggatt attaaagctt
     6001 tacttacctt ttgaccgaac catttcaaat ttgacaaaaa accagtataa taaggaagta
     6061 tcgaaaaaat atccataaaa ggaatgtgga aaatggacaa ccagctgaca aaagaactaa
     6121 aagaaaacta taacatcgtt tttcacgatc taaatttgct tgaacaagct tttacccatt
     6181 catcctatgt gaatgagcat cgcaatctgc aattatcgga taatgagcga cttgaatttt
     6241 taggagatgc cgttttagaa ctgatggttt ctcagtattt attccgttta tatcctcatt
     6301 tgccagaagg aaaattgaca aaaacacgag ctgcgatcgt tcgcgaagac agcttgtcga
     6361 aatttgccaa agaatgtcac tttgatcaat atgttttgtt gggcaaggga gaagagaatt
     6421 caggaggacg tacgcgacca gcattgcttt gcgacctatt cgaagctttt ttaggcgcgt
     6481 tatatcttga ccagggattc gacacgaccc atgcattcat cgaaaaagtg atttttccta
     6541 aagtcaaagc aggtgctttt tcacatgaga tggatcataa aacaaaacta caagaagtct
     6601 tgcaaaaatc aggtgatgta tcaatcgaat accgattgat caatgaagaa ggtccagctc
     6661 atgaacgagt attttggatc gaagtttatg tagatgatca attgattggc actggacaag
     6721 ggaaatcgaa aaaattagca gaacaagctg cggcggaaaa tgcattggca gcactgtaaa
     6781 agtaagtgag ggaaagcttt gtatttgaaa cgaattgaaa tagccggttt taaatcattt
     6841 gcagacaaga cagtcattga ttttgaaaat agcgtcacag cagtggttgg accaaatggc
     6901 agcggaaaaa gcaatattac agaggctatc cgctgggtat taggagaaca atctgctaaa
     6961 agtctgcgag gcggaaaaat gccggatatc atctttgccg gttccgatac ccgtaagcag
     7021 ctgaatattg cagaagtcac agtgatttta gacaatagtg atcattatct gccattggag
     7081 tacaatgaga tcagtgtaac ccgccgttat cgacggactg gggaaagcga gtttttcatc
     7141 aataaacaaa gctgcagatt aaaagatatc caagaattgt tcttagattc tggattagga
     7201 aaagaatcgt tctcaatcat ttctcaagga aaagtagaag cgattttcag tagtaaaccg
     7261 gaagaccgtc gtgggatctt tgaagaagca gctggtgtct tgaaatacaa gcagcggaaa
     7321 aagaaagcag aacagaagct ttttgaaaca gaagacaacc tcagccgcgt ccaagacatc
     7381 atccacgaat tggaagaaca gttaactcct ttggccgccc agagtgaggc agcaaaggag
     7441 tttttacggc taaaagaaac actgactcaa accgatgtct ctttgatggt tgctgaaatc
     7501 aagacagcaa aaaaagattg ggacaacaaa caagcacagc ttgcgaaatt caatctagaa
     7561 ttaggcaaac tatctgagtc gatccaagaa caagaatcca ttttggcgaa gcagcgtaaa
     7621 gaaaatgcgc aagccgatcg tctgatcgaa aaaaatcagc aagtattatt agatctttcg
     7681 gaaaaactga agcagacaga aggacaaaaa gatgttttgc aggagcggac aaagcataca
     7741 caaaaaagct cgcaagaata tcaaacttca ttagcagaag cacaaaaaaa ggttaaacat
     7801 tttgaaaagc ttcaagaaag cttgatgaaa gcagcagcag aaaaagaaac agagatccaa
     7861 aaagctgaag caaatctcat aaaaacacag caagaattag aaaaatacca aaaatcaacg
     7921 aaagaattat tggcagaatt aagagatcaa tatgttgatt tgatgcaaga acaagcagct
     7981 gtaggaaacg aactgaagta tctagaacgt caatatatac aagaaacagc aaaaagcaag
     8041 cagacgttag ccaagcaatc ggaagtagaa gcatcagtag accgattgat cttacaaaaa
     8101 gaagaattga cacaaaagca ggcgcagcta aaatcatctt taactgaaac aaaagagaag
     8161 ttagaaatga tccaacaaaa cgggaaaaag ttccaagaag cattagcaaa agaacagcct
     8221 aaaatgtatc aattaatgaa tcaagtccag cagctacgtg ctcgtaagaa aagccttcaa
     8281 gaaatccaag aaaactattt tggtttttac caaggggttc gattggtctt gcagcataaa
     8341 cagcagcttt cgggtattgt cggagcagtg gctgaactga tcgatgttcc tgcagatttc
     8401 acgttagcta tcgaagcagc tttaggtggg gcagcccagc atgtcatcgt tgaaaatgaa
     8461 aaagatgctc ggcaagccat cacgtatttg aaacagcaaa gaggaggaag agcaacattc
     8521 cttccactga cgacaatcaa accaagacaa ttaccggctc atatattgac acaagcagca
     8581 gctgtagaag ggtttatagg gattgccagt gaacaagtca gttatcccga tcaaatccaa
     8641 actgtggttc aaaacctgct aggaacgatc cttttagcca aagacttaac tagtgccaat
     8701 gcaatcgcgc aaacgatccg ctatcaatat cgagtggttt ccttagaagg tgatgtgatg
     8761 aatgccggag gatccatgac aggtggagcg aataaacgag gaaaccaagg cagtttgttt
     8821 gtacaaaatc aagaattgaa acagttgact agtgaatttg aagaagcaga taaacaactc
     8881 caagcacaag aaaaaaaagt tcaggagctg caacaagaaa cagctcgttt agccgaagaa
     8941 caagaagttt tgcgtacacg aggcgaacaa ttacgttttg aagaacaaga agcgacaaat
     9001 cagctacaaa acatcataaa tgaacttgaa cgttttgaaa aagaaaagca aatctcaact
     9061 tttgagacac gtgaactgca gcagtttatc gaagattacg aaaagcaaac gaacgaattg
     9121 aaagacaaac aaacagatct tgaatcgcaa cgacaaaaaa tcgacgaaga aatcaaatcg
     9181 ttaagtcaag aaagcgacca gatggaagcc agaagagcac aagtacaaag tcaaaaagca
     9241 caagaacaag cagatcttgc tgtcttgaaa gagcagttta atcatctgca gatccagctc
     9301 cgcggtgccc gagtccaaaa agcagaggca accgaacgtc aagaagcgat agaaaagcag
     9361 ttggcgacat tgacagcaga cttttctgat catgaagtca cagaagaaag tttggaaaaa
     9421 caaattaacg aattatctgc tcagcgagaa acattaaaag ccgagttggc aaaagccaaa
     9481 gagcagcgag atagaaggca aaaggagatc gataagcttg aagcagtctt ggcagagcgt
     9541 aatcgtgagc aaaaagcacg actgtcggaa caatccaaat tagaagtcca aaaagaccga
     9601 gctgaaatga ttttagataa tcatttgacc tatctgcagt cagaatatca gctgacattt
     9661 gagaaagctt cccaagacta tcaagaaaca accgatatcg aagacagtag aacaaaggtt
     9721 tcatccttaa aagaacagat tgaaaaactg ggaccagtaa acttaaattc aatcgaacaa
     9781 tacgaacaag tcagtgaaag acatacattc ttagcaacac agcgagatga tctgttagca
     9841 gcgaaaaacc aattgtttga aacgatggat gagatggatg atgaagtgcg gacaagattc
     9901 aaagaagtat ttgaagctat ccgtcaagaa ttcaaaattg tctttccaaa tatgtttggc
     9961 ggcggtaaag ctgaattagt actgacggaa ccaactgatt tgctgaaaac aggtattgag
    10021 attgaagtac aacctcctgg caaaaaactg caaagtctaa gtttgctttc tggaggagaa
    10081 cgagcactta cagccatcgc gttgctcttt tccattatcc gtgtctgtcc tgttccgttt
    10141 tgtatcttag atgaggtaga agcagcgtta gatgaagcca acgtgaaacg ttttggtcgt
    10201 tacttgagcg atttccaaga tgacacacaa tttatcgtag tcacacaccg caaaggaacg
    10261 atggtcgcag cagatgtact gtacggagtg acgatgcagg aatccggtgt atcaaaaatc
    10321 gtttctgtac ggatggaaga tatcaatgaa gaggcgaaaa tcaacgaaac gaaagtgtag
    10381 gaacgaaaat gatcaaatta gtagcaatcg atttggacgg tacattatta aatgaacaaa
    10441 agctgatttc agatgagaat aaacaagcat tagcacaagc aaaacagcaa ggggtcaaga
    10501 tcgttctatg tacaggacgc cccttagctg cgatggcgca ttatctgcaa gaattaggac
    10561 tggtagatga aggtgactac agcatcactt tcaacggcgg tttggtacaa aaaaatgata
    10621 ctggtgaaat catggaaaag aaagtgatgg aagtatcaga tatccaacgc ttatacaaac
    10681 tggctcagca attggatctg ccactagatg ttttgtctga caatgtggtt ttacagctgc
    10741 ctagtgcccc aaggaagcaa tccttgtaca atgtgttgaa taagctgctg caattccaac
    10801 cagcggatat aagcgatatt acagatgaat ttgtgcttaa taaggcagta atcgcttatg
    10861 accaagaaga attagatcct aaaatcaaag aaataccagc agaattccat gaaatgtatg
    10921 agatcatcaa aacaagaagt atgcttttgg aatttatgcc aaaaggtgtt acaaaagctt
    10981 atggtatttc tttattggca aaagatcttg gtttgaaaca agaagaaatc atggcgatcg
    11041 gtgatgagga aaatgattta ccgatgatcc aatatgctgg actaggcgta gcgatggaga
    11101 atgctgtgcc tttcgtcaaa gaagcagcag atttcgtgac agccagcaat gaagaaaatg
    11161 gcgtagccaa agtgatccaa aaatttgtat tagagtaggg ggagtcaaat gggatttttt
    11221 gataaaatca agcgtgcttt tactggcgaa gagaaaacag aagaagttga agtacaggag
    11281 aagtacgaca agggtttaga aaagacgcgt aaaacatttg gtgaacggat gaatgagttg
    11341 tttgccaatt ttcgaacagt cgatgaagac ttttttgaag aattagaaga aacacttatc
    11401 ggtgcagatg taggttttga aacagcgata cgaatcaccg aagcattacg tcaagaagtg
    11461 aaactcagaa atgcgaaaaa atcggcagaa gtccaaaatg cgatcatcga gaaacttgtg
    11521 gatctctatg aagcggaagg aatcaatgaa gtaaatgaac taaatatcca agaagacgga
    11581 ctaacagtta ttttgtttgt cggtgttaat ggtgtaggga aaacaacaag tattggtaaa
    11641 cttgcccatc agttcaaaaa cgaaggtaaa aaagtattga tggcagcagc agataccttc
    11701 cgcgctggag caatcgacca gttagttgtc tggggcgaac gcgctggtgt tgaagttgtt
    11761 cgtggcaatg caggaggcga tccagcagct gtcgtattcg atgctttgga aagagcaaag
    11821 acagagcagg cagatgtttt actagttgat actgcaggcc gactgcaaaa taaagtgaac
    11881 ttgatgaacg aattagaaaa aataaaacga gtaatccagc gggaattacc agaagcaccc
    11941 caagaaacac ttcttgtatt agatgcaacc actggtcaaa acgcgatggt tcaagcaaaa
    12001 caattcaaag aaacaacaga tgtgaccggt cttgttttga caaaattaga tggtacagca
    12061 aagggcggta tcgtacttgc tatccgtaat gaattgcatt taccagtgaa attggtcggt
    12121 cttggtgaag gaatcgatga cttggagccg tttgatccaa atgattttgt cgttggattg
    12181 ttcaaaggat tactaaaaga agaataaccc atgtctaaaa agcaggagct gtgacaaaag
    12241 ttgcagctcc ttgtttgaat gattgttttt aacatagctg tgtttttgat gccttcattt
    12301 ttatacttat ttgtaagcgg ttctcaaaca atttcgttct ttttcttaga ctttttgatt
    12361 ataattagat tcatatgaat tggaggtcac ttaatgatga attttaagca attattattg
    12421 catgtcaatg cgcgcccttt tatcgaccaa gctcgatttg gtatagaacg ggaaggacag
    12481 cgggttgatc ttgcaggaaa tctagcaaaa accgatcatc cagcaatctt tggcgatcga
    12541 tcctatcacc cctatatcca aacagatttt agcgaaacac aaacagagat gatcacccct
    12601 gttaccgatt ctattcccga attatttcag tatctcgctg ctgtttatga tgtgactgct
    12661 cgttctatac cgaaagaaga gatgatctgg ccattaagta tgccacctgc cttaccggaa
    12721 aaagacgaag agatcattat tgcaaaatta aaaaatttcg aagatgtctt gtatcgacgt
    12781 tatttagcaa aaaaatacgg gaagcgaaaa cagatggtaa gcggtattca tttcaatttt
    12841 gaatttggcg atgagttgct aagaacattg ttcagccatc aggaagaatt tcaagatttt
    12901 tccgaattca aaacagaact ttatttgaaa acagcgagaa actttatgcg ctatcgctgg
    12961 atgatcactt atttattcgg tgcttcacca atgagtgaga agaactactt tttagatgaa
    13021 tcacatccgc aagaacctgt tcgcagtatt cgcaacagtg cgctgggcta tacgaatcat
    13081 ccaaatgtga aagtttccta tgcttctatg aaacagtatt tagcagatat cgagcgaatg
    13141 atcgaagaag ggaaactttc ggaggagaaa gaattttata caccgcttcg tttccgaggc
    13201 ggaaagaaag tcgcagattt agcaacaaca ggtgttcgtt atattgaatt gcggaatatc
    13261 gatttgaatc cttatgcaag attgggtatc aatccagagc aagttcggtt cttacaatta
    13321 ttcctgatgt acatgttatg gacagaagaa aaagaagact gtgaccaatg ggtagcagaa
    13381 ggtacgactc gaaataacaa agtagcacta gaacaaccat cagatcaaac agaatttcat
    13441 caagaaggta gagaaattct tgaaggaatg aagcaaatgc tggttgaatt ggattggttg
    13501 gattctcttt atttagtaga ggaagcattg actcaaatgg atcatcctga acaaacgtta
    13561 gcagcgaaac tctatcaaga agcacagcta tcgagtcagc aagaagttgc tgtggcattg
    13621 ggacatcaat attataaaga aagccatgaa cgtccctatc agttggccgg ttttcgcgaa
    13681 atggaacttt ctactcagat atttatgttc gatgccatcc aaaaaggtgt ccaagtcaaa
    13741 gtattagatg agtcggatca atttttgcgt ctgcaattcc aagaccatgt agaatatgtg
    13801 aaaaatgcaa acatgacaag taaggacagc tatatcgtcc cacttatcat ggaaaacaaa
    13861 acagtaacga aaaaagtctt aaaagaagct gggttccggg taccaggagg cgcagaattt
    13921 tcatccatgg aagaagcggt aaaagcttat ccgagatttg ccgagcaggc atttgtgatc
    13981 aaacctaaat caaccaatta cgggttaggt atcacgattt tcaaggaagg agctagtctg
    14041 gaagattatc aagcaggttt agccattgct tttcgtgaag acagttcggt tttagtagaa
    14101 gaatttatgc cgggaacgga atatcgtttc tttgtgatcg acggagaagt ccaagccatc
    14161 atgttgcgag tccctgctaa tgttattggt gacagtatcc gaacagtgaa agaacttgtt
    14221 gaagaaaaaa atagtgaccc tttgcgtgga acgaatcatc gtgcaccttt agaattgatc
    14281 caattaggtg aattagaaca attgatgttg aaagaacaag ggttgacgat cgaatcagtt
    14341 ccccaagcca atcaaattgt ttatctaagg gagaactcaa atatcagtac aggtggtgat
    14401 tcgatcgata tgactgatga attttctgaa gcttacaaaa aaatcgccgt ttctgcggta
    14461 gaagcactag gagctaagat tagtggtatc gatttgatta taccagataa agaaatcgat
    14521 cctacaactg ataaaaaagc atatgggatc atcgaagcaa atttcaatcc agccatgcat
    14581 atgcatgttt atccttttgc aggaaaaggc agaagattga cgatgaatgt attgaaattg
    14641 ttatatcctg aagtatttta aaagaaaaag cttttgacag attgtcctat ttgatgtcaa
    14701 tctgatcaaa agcttttttt gcatgtaaaa atttatgtgt tttttcgttt taaaataaac
    14761 gatcccagaa gctttttttc ttttttggtt tttctttttc agctggcgaa gagttttctt
    14821 ctgggtattt tttttcgata tcaatcacag gttcatttac tgcttctttt gctgttaaga
    14881 gcaatccgaa ttcctcagga tttgcagtga cgtaatcgtt gactacagtg aaacggctat
    14941 tgtttttcgt tgctagctgg atatattggt tttggacaga aaggggtaga gccccattca
    15001 agagtatgga agcatctgga tgttttttga gttcttttaa aaagttttct ttgttttttg
    15061 gttggatcat ttcagcaaca gtcatactaa gatagcagcg ttccctgaat gtccctaaat
    15121 atttatgctg ttcttcaggg ttcactaaag gtgtaccata aagtccctta tccaaatggc
    15181 tttttacatc atcttgtgcc ataaaaaata ccctccttct tctctttgtc tttcttacga
    15241 ttatagcata ttcccaataa tacctaaaac atatgaaaaa gcggttgctt ttgttcaacg
    15301 attcacttaa aataagaatg aaaatcattc tcaattagaa aggtggattg gatgcgctac
    15361 ggacgactag tagcattatt agttatttta agtagtattt cattgttcat cggagtatcg
    15421 aatgtacaat tgtcagaact gctccagtta gatgggcaaa gcttacaaat cttgctagtg
    15481 actcgtttgc cacgaacgat ttgtttgatc cttgtgggag cgaccagtag tatttgtggg
    15541 ttgatcatgc aacatttgac ccaaaataaa tttgtttcac caacgactgc gggcacgatg
    15601 gatagtgcgc gtttaggtat tttagtggct atgattttcc tacctggtgc atccttgatt
    15661 gtccgatcct tgacagcatt ttgctttgct tttgcaggaa cattgctttt tttatccctt
    15721 acgcgattgt tcctccagaa aaatcaattg atgcttccgt tgattggagt catgttaggg
    15781 aatatcattg gttcagttgc tacttttttt gcttatcaat tccagttggt acaaaatatg
    15841 acttcatggc ttcaagggaa ttttgcgact gtgatacgag gaaattatga gttattgtat
    15901 gctgttgttc cattgttgct cttggtttgg ctatttgctt accgatttac gatcattggt
    15961 ctgggcgaat cgtttgcagt aaatctggga atagattacc agaagatgca gtttttaggc
    16021 attggcttag tatcattggc tagtgcagtc atgttgatca tggtaggaag cattcctttt
    16081 atcggagtga tcattccgaa tcttgtctct agaatatatg gagatcaagt gcacaaaaca
    16141 attggcgtaa ctgctttagc aggtagtttg ttcttagtat tttgcgatct tgtcgctcgc
    16201 gtgttgattt ttccttatga aataccagtc agtgtgatcg ttggaatcat cggtggggct
    16261 atcttccttt atcttctgat aaggggacga cgatatgctt aaaaaaaagc catctatttt
    16321 tattggtatg cttgttcttg ttttatttgt tacaggaatg tacttgacga tccatacata
    16381 tggaaactgg cattttgcat gtgcattgag gagtaagaaa atttttgcat ttatccttgt
    16441 ctcgctagct gtcagcagtg ccacaatcag ctttcagaca attacgcata accagttttt
    16501 gactccttca gtattgggac ttgagtcgtt atatgtcctt gtccaaacgg tccttttctt
    16561 ctttatcgga ggagtgacga tgcttcagca gacaggtaca gtttttttct tattgaacgt
    16621 tgggctgatg gtcgggctga gtctattgtt atttttcttt cttttgaaaa aaatgagagc
    16681 gaatcttttt ttattactga tgtcagggat gattttaggt acgctttttg gtagtgtcag
    16741 taccttttta caagtcatca tggatccgaa tgaatatgat ttgcttcaag gcagattatt
    16801 tgcaagcttt ggcaatatcc agagtcagta tcttttgcct gcttctttga tcattatcgg
    16861 ctgctggtac ctgttatttc gatatcgaca tgtgctagac gttttccatc tcggaaatga
    16921 acaagcagta aacctcggga tagacacaga tcgttttgaa tttttgattt tatgttgtgt
    16981 cagtcttttg acagggacat caacggctct tgtgggtcca gttacttttt tagggtttat
    17041 tgtcgcaaat atcagttatc gtgtcttttc tacttatcgt catggtcagc tatttatcgg
    17101 aagctttctg gtagctatgc tgtttctgac aggaggacag ctcgcagtag agcagatttt
    17161 cggctggaat acaacagtga gtgtcgtcgt tgaatttgtc ggaggaattt attttgtcac
    17221 aaaattgatt cgcgaaagga agagtatcct atgattgatt tacaaaaggt caataaactg
    17281 taccatggaa aatcagtggt taaagatgta cagttaacga ttcctgaaaa gaagttgact
    17341 gcttttatcg gtccaaatgg tgctggaaag tcaacggtgt tgtcgatgat cagtcgtttg
    17401 ataccaaaag atacaggtga aatctattta gaccataacg aagtaaaggc ttggaaatcc
    17461 aatgaattag ccaaaaaatt atcgatccta cgacaatcga atgaaataaa tgtccaaatc
    17521 accgtgaaag aattagtaga atttggacga tttccttata cgaaaggaaa attgacgaca
    17581 aaagacgagg aaatcgttca ggaatcactg gaacaccttg ggttagaaga aatagcggat
    17641 caatcgattg acactctttc aggcggtcaa ctccaacgtg cgtatattgc aatggtgcta
    17701 gcgcaagaca cggattatat tttactggat gaacctttga acaatctaga tatgaatttt
    17761 gcggttcaaa tcatgcagat tttaaaaaat ttagtacaag aaatcgggaa aacaattgtt
    17821 attgttcttc atgatatcaa ctttgctgca agttatgcag acgaaattgt tgcaatgaaa
    17881 gatggacagt tatttacgca aggaacgacc caagaaatga taaaaaaaga tgtattggat
    17941 gaattatacg aaatggacat acggatttgt gagattgaag gccgcagatt ctgtctttat
    18001 ttctaaaact aacattagga gagaagagat gaaaaaatta gtagtaggta tcttgattct
    18061 agcatcattt ggtttagcag catgtggaaa tagcaacaac accagtcagg cagacacaaa
    18121 aaaaagcagc acacaaactg aaactacatt gacgataaca gatagtaatg gagaccaaat
    18181 cgaggtgcca aataatccag aaaaagtcgt cgtattcgac aatggttctt tagatacgat
    18241 ggatgctctt ggtgttggag ataaggtagt tggagcagct acaagcagcc ttcctgagta
    18301 tctttcatcc tataaaaaag tagaatcagc aggtggaata aaagaaccag acttagaaaa
    18361 aattaatcag cttcagccag atctaatcat catttctggc cgacaaagag attttcaaaa
    18421 agatctaagt caaattgctc cgaccatttt cctttcttta gatgcgaaaa atccatggga
    18481 atctttccag caaaatgtca ctgctttagg ggagatcttt ggtaagcaag aagaagctaa
    18541 aactcaatta gaggaattat ctagtgcaat cgatcagacg aaaaagaaag cagaagctac
    18601 tgacaaaaaa gcactagtca cacttgtgaa tgaagggcaa ttatcagcct acggatctgg
    18661 ttctagattc ggtttcatcc atgatttatt tggctttgaa caagcagatg atcaaatcga
    18721 agcatccact cacggtcaaa gtgtttctta tgaatatgtg ctggaaaaaa atccggatat
    18781 cttatttgta gtagacagaa caaaagctat tggtggagac gacagcaaag atgatatttc
    18841 tgccaatgaa ctggtagcac aaacgaatgc tggtaaaaat cagcagatca tctcccttga
    18901 accagatgtc tggtatttaa gcgggggcgg tttagaatca atgaaactga tgatcgaaga
    18961 tgtgaatcaa gcatttaaat agcataaata acagaggatt aaactctcat actatgtaac
    19021 aataatgttt cttacattct taaatagcaa gagcctatcc tcaaagaagt tatcaaaaac
    19081 catccgtaaa atcgtattga tgattttacg gatggttttt atttatttga gaaatctttt
    19141 ttcaacgtga attcattatc tacatcatac gggttgattc tagaatgggg agtggcaaaa
    19201 aatagtgtga tccatttttt cagcatcaag atcgcctcct ttgatttgaa tcaaaggtac
    19261 aaagcgaaac ttagataaaa cttaaattta gatattttta ttttgtaccg atctcttctt
    19321 taaacgatgt tccatctgct gcttctcggc aataagtcca ctagactaga aaatatgaga
    19381 agctattttc tgtccagcgt tcttattact caaaacaaat cgcagttgtc acaatctctt
    19441 ctaaacgatg ttccatctgc tgcttctcgg caataagccc actagactag aaaatatgag
    19501 aagctatttt ctgtccagcg ttcttattac tcaaagcaaa tcgcagttgt cacaatctct
    19561 tctaaacaat gttcaatcag ctgaccttcg gtattagctc aaagcactga tttcacaacc
    19621 tctatttagg catgtgtctc agcaagcggt tgatagttga tgatctgcat ttttcgataa
    19681 aaatcggcgg gaagcttact ccaccgtttt agtaattgat agaaataact ctcttctgct
    19741 ttgattttat actcgcttaa tatcaatcca aaactattcg ttttttttga agctaatttt
    19801 ttataaatat ccaatgcttt ggttttatct tcattgacaa agagtgtttt tacttcaata
    19861 taagaaccct cgatattttg tgtttcttcg ataaaataga tgttttttat atattccttc
    19921 atagtgattc ctccaaagaa gtcttttatt tgataagttt actctaccga agagttgtta
    19981 ataatccgtg aacaaaaagt aattcttttt ccaatagttg ttaaaggttt tttatttttt
    20041 atagtatttt ttgttattta agaaaggaat aaggttaaac aaataacagg ttgctttttg
    20101 ggtctttttc cgttcaagaa actgaacgaa gaaaagggaa agttgtctct tttgtgataa
    20161 gagaaatcat aataaacgaa tgacttactt tccaataaat tgagaacagt cagaaccaga
    20221 caaattagtt gcatctgatg aataaagtga ttattctatg acatgaggtg atacagatga
    20281 aattagaagt gacaaaagaa gcacaagaag tattgaagaa taaattagaa gcagacgatc
    20341 aacttttatt agatatcgaa aacggtgacg gtccatttgc agaaagccaa gtcagttgcc
    20401 aattggatac ggcatttcga ttgatcattg tcaaaaaaga tacacaaaca gatctaagtc
    20461 tttattctgt agtggctgat acaccagttg gaccaatcaa aatcaaagac agtgcaatac
    20521 tttacatgga tgatccatcc acacttgctt tagaaccaac ttataatagt ttacaattaa
    20581 aaggaccaag cggcttgaga aaaggcaact tgcaagttgt tcgaaaagac tagagggata
    20641 aaatgggaaa attggcgatc atcagcgacc tacatgtgga catcaataaa ttcggcgaat
    20701 cagagctgat gcagctttgg caagttttgc aaaagcacaa gatcactcgg cttcatctag
    20761 ccggagatac ggctaataaa gtagaccgtt gcctagctgt tgtcgaattt ttcgtcaata
    20821 aaggattatc tacgacattt catttcggca atcacgagtt ggcagatgtg cccaatgaac
    20881 agatgatgga ccattttcca gacccacatt ttttaaatga acggtatatt gctttaaatg
    20941 agcagaccgt tcttttaggg atgaatggct ggtacgacta tcagttttcc gaattgaaag
    21001 attacgaggc aatcgtacga atgaagcgtt tgtattggta tgatcggttc atccagcgtg
    21061 ggaaaacaga tctcgaggtc aatgcagaaa tgcttgcagc tgcaaaaaat ttgctagacc
    21121 agctggaagc aaaaaaactt gaggtgattt tatccactca tttcgttcct aaacgggcat
    21181 ttatcatgta tcaatctgct ccttatgagc gttggaacaa attaaatgca tttttaggat
    21241 cagaatcatt cggagaatta cttgaccgtt attcaaatat caaacaagta gtttttggcc
    21301 atacgcatcg tcgctttgaa gataaatcca tcggtcacac gatttacagc tgcagacctt
    21361 ttggttatta ttatgaatgg cagctgaccc gagattttgt cttgaaagaa cagttgctag
    21421 acagctatga tcctatgaaa ttacgtgtcc tcttgcgtgc ccatcaccca gcttttcagg
    21481 cctatcaaac acaacatttg caagaggaat ttgaacaagc gatgacaatc gttccgtatt
    21541 gagacagcat tagtaatgtg taaaagaaaa taccttgtaa aacataagga taagtaagag
    21601 cagtagatcc atttatcgtg gatatgctgc tttttttgcg gaaaataatc agcgaatgtt
    21661 tatttgatcg gatgatttgt aactcgcaga gtactagatg caagcgtgta ttcttttttt
    21721 gaacgataat cacaaaaaaa aatataatat acatttttta tttaattata attaatattg
    21781 attgaagtct aggcagtcaa gtcacatttt gatagatagg agggggactt acgaattatt
    21841 ttaaaggcta tgacagctgt atttctagat tgtgtttgca tcaagttgca ataaaaacta
    21901 gaaatcaaat cgggctgata atcatttata actaatcgat acataggacc aaatataggt
    21961 ctatgttctt ttatttcata agtatcgtaa aacagaattt tgctatggat taatccaatc
    22021 gactgatatg tttagtaaat aaagaaagag gaatgtcaat gatgaaaaaa gaaatcgtat
    22081 taacagcagc aatgatgtta ttcagcatgg tagcaagtac gacttttgtt tctgcaactg
    22141 aagtataccc aaaggaatat aacacagaag gaacgattac atttgaagca ggagatgagg
    22201 gtgtaactcc tcctgttgat ccagagaatc ctgatcccaa taaacctgtc gatccatcag
    22261 atcctccaag ccctggaaca ggaggggcat tatcgattga ttatggatca aaattcaaat
    22321 ttgggactca aaaaatttct actgcggata aaacgtatta tgcagctgct gatgtgatga
    22381 acgatgggtc tagaaaaccg acatatgtcc aagtgacaga taggagaagt accttatctg
    22441 ggtggaaatt aagtgtctct caaccagaac agtttaaaac tgccagtggg gatgaactag
    22501 ttggagcaca gctgaaattt acgaaagggc aggctgtttc tttggttgat ccaacatata
    22561 cgccgcaaac ggtgaattct gaattaacgt tgacaccggg aggaaacaat acgttagcaa
    22621 ttaatgctaa atcaggaaca ggtgtgggga cttgggtcta tcgatttggt gcaaatgaaa
    22681 atgaaaatca agatgcggta cagctttcgg tacccggtaa atctgtcaag ttagcgcaac
    22741 aatattccac taaattagtt tggacattag aagatacacc taacaactag tttactgaaa
    22801 agaactgtta aaaataaata cgtagtcata ttgtcttgcg aggtaatcga gcaaaaattg
    22861 tcaaaggcta ggctaaattt gtttggatac gaatagttca tctaggaatt taacgatcag
    22921 gaggaaaaga tatattgaaa aagaggagga tcgtgctcat tttaagcagt ctcttagtga
    22981 ctaatatctt tccgatcata gggtactctt tggaattgaa tcagttggag catggaaata
    23041 gaagagacga agaaagcaaa ctcacttcga cattcgacag tttaatagaa tccaaccaga
    23101 cacaagaaag cagtgaggat ttaagtattt cgagtagtgg agagaccact gacaacgtag
    23161 aagctgaggg ttcggacaaa caaggactag aagatgcacc aatttctgcg gttgagctgc
    23221 ctgacgatcc caatattatt tctatcgata aaatttttca aacacctatc ggtactagca
    23281 cgtccatatt ggaaaatggg aagttactgc agttgaaccc agcagaaagg tctcaaagag
    23341 gagctatttg gtcaaataag aagattagtt tactctcgga ttttacattt aaatcttatt
    23401 tatacttagg aaatcaacga gatagagcgg gagatggcat gacctttaca ttgactggcg
    23461 atcctagaat ggctagtaat cccagtcaag ttattggatc gccaggtatg ggaattggct
    23521 catattctac tcgttctggc caaccttaca ttgcgaatgc gctctcgatt gagtttgata
    23581 cgtataaaaa tagtggttct tctgatcgaa tggatcgaga gataactgct gataatagag
    23641 ggcatggaca tgtcgctttt gtcacgccta aaccaaataa taataattat tctggagagc
    23701 atactggcgt gacggtagct cctacatttc tttctaatgg cacatggcgc ttgttaataa
    23761 ttaaatggga cgcggagcaa agaagtctta cttatgattt ggaaggtgtc ggtcgctctt
    23821 ctcggattat acctgatctc attaggcagt ttggttctac agaagtaaat tgggggttta
    23881 cttcttcaac aggtactttt tttcaagaaa atgctgtcgc tgtcacgcga cttccatcga
    23941 atgtcacatc aacagctgat gtaaaagtca acgatagtga ttatggggcc atcgctgaag
    24001 ctggaggaaa cgataagata actatcagaa atacattgaa ggtcgagggg gattttcttg
    24061 aatataagaa aatgcagact agtatcgacc tcccacaaga attaactatt cccgagcata
    24121 tcttgatcaa tggaaatcaa attgatgcca aagatatctt gataaccgga aatcgagtca
    24181 cgctaaatct gacagattat tttattgctg gagatacgtt aataatcgaa ttggagacaa
    24241 ctttgcagga agtaattcct gaaaaagtac tcactatgca ttttgaatac cttgaaaacg
    24301 ggaatttgat tgagcaatcg aatgaaataa acctaaagat cgcaaagctg aaggagatag
    24361 atatccatgt ttattataaa gataaagaca gtcagattga tattgctgaa atgaaaacaa
    24421 tcactgggaa aataggagag aagtataaag aaactcctat tgatattgaa gggtatgttt
    24481 ttgactcgga ttcagcgaat acagaaggca tcttctccga aaatacagag gatatctatt
    24541 tttattacag aaaaggcaaa ctttatctct caactgctcc tcaatatgtc aattttggaa
    24601 aacataaaat atccagtaga ccattaagtg aattcggaca gttttcaggt gggttgaaaa
    24661 ttatagatga acgggctttt ggaagttgga aattacaatt gaaacaagag aaagttctta
    24721 caaatggaga ttttgagctt ccagaagctc tttcctttgt tacctctgaa aatacaacgg
    24781 ttatagggac atccgctgtt acgattttcg aaagtgatca aaagggtgaa agcgatttgt
    24841 cctctctttt agattctaca gctcatcgtg gaatcagaat aaatattcct gtagaaaacc
    24901 aaagaatcgg aacctttgag gggaaactat cttggatact tgcagatgtc ccaggaaatt
    24961 aacaggaaga gaagaatata acaaaattat tgagtttcaa gaagggcaca ggtaccatag
    25021 gctcttgtgt cccacagaga caagattttg ttatagtctt ctcttacata tgattaaagg
    25081 agcgattttc ttgagaccaa aacaacaatt atttataaca ctcctagtta ttttaggggc
    25141 actttttaac aatatatcag tgtcagctag tgcatttaat ttttcagtga cgccgataac
    25201 ttcagagaat caaatcgaca agagaaaaac ttacttcgat cttcaacttg atccggatca
    25261 ggaagtagaa gtaaaagcgg aattgagaaa tgatacagaa aaagaagtga aaatcgatat
    25321 ttctgtgaat agcgcaacca ccaattcaaa tgtgatggtt gaatacggaa aaaacgagat
    25381 tgaaaaagac atgagcctga tctttgatct ggtagattat gtgagctatc cacagatagt
    25441 gacgctaaaa cctaaaagtg tccaaaccgt tgcttttcat gtcaggatgc cgaatgagcg
    25501 atttgatggt gtattggctg gtgggatcac attccaagaa atccagacgg aaaaggacca
    25561 aacagaaact aaggatcaaa gcttatcgat tgagaatgaa tacacctata ttattgcttt
    25621 gttgatgcaa cagacactga acgaagttgc tccgaacctt actcttcatg aagttaaacc
    25681 agatcaaatc aatgcaagaa atgtcatctt agcaaacgtt caaaatgatc aaaaaaccta
    25741 tatcaatcaa gtagttattg aaacaaaaat tacaaaaaaa ggacattctg aagttttata
    25801 tcaagaggaa aaagaaggat tgcagattgc gccgaataca aatttttctt ttccaactgc
    25861 tttaaatggt caaccgctaa ccccaggtga atatcacctt acaatgactg ttttaggaaa
    25921 tgaaaatgaa aatggaaaat tttcgagaaa aaaaggaaat actacgatca actataccaa
    25981 tcaatgggta tttgaaaaag attttacaat agacggaaaa gtagccaagg aattgaacac
    26041 gacggatgtt acgattaaaa aagacaataa ttggatttat atcttagtag cttcactttt
    26101 cttattgttt attttattat gcatactttt gtttgttttg agaaaaaaag aagagaaaga
    26161 aaagtaaggt taagataaca aatagtaaat ttttattatt gtaggatggt tgggctcttt
    26221 ttataacaat ataaaatata ccattttatt aataaaaatg catactagta aatgatataa
    26281 ttctaataac aggtttgatc gcgagaaagg ccaaataaaa cgctctccaa aatgttaaaa
    26341 aaaagacagg acgatgtata ttatacaaac aagaatcgct gacaacagtg actcggatcc
    26401 aggaaatcag ttttatcccc agctagttgg tgaatgcatg gaaagtaata tacgtagaaa
    26461 gagctgatga ctaatgaatt atgagttttt tttaagcaaa gaagatcaca gaagttattc
    26521 attacttaaa tatttagaag caagctcaaa tttatcagag tcaatatcga atgttcaaga
    26581 agagttgtcg ctatcgacat ttctcttgaa aaaaacgatc gataagctga aacatgattt
    26641 acaaaagttc gatctagaga aaaatttgcg agtaaccgtc agtgaattgg atatatgtct
    26701 ggaaattaat ggaaaatgtt ctagcaaagc tctgttatct cgttatataa cggattcttt
    26761 atctatgaaa atggtattgg ctttctttca ggaaaaatat atctcagtag aaaattttgc
    26821 taaacagcat cacgtgagtt attctgtggc ttataaagta ttacaaagac taaaaagaaa
    26881 tctaaaaaaa tatcaaattt tttttgaaaa gagaaagtt
//
LOCUS       DACCXM010000025        26822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCXM010000025 DACCXM010000000
VERSION     DACCXM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.027"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            69..743
                     /locus_tag="IVY61_000912"
                     /pseudo
     CDS             69..743
                     /locus_tag="IVY61_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1000..2199
                     /locus_tag="IVY61_000913"
     CDS             1000..2199
                     /locus_tag="IVY61_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7970044.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            2221..2433
                     /locus_tag="IVY61_000914"
     CDS             2221..2433
                     /locus_tag="IVY61_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7970045.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            2508..2984
                     /locus_tag="IVY61_000915"
     CDS             2508..2984
                     /locus_tag="IVY61_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP7970046.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            2981..4789
                     /locus_tag="IVY61_000916"
     CDS             2981..4789
                     /locus_tag="IVY61_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7970047.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            4866..5081
                     /locus_tag="IVY61_000917"
     CDS             4866..5081
                     /locus_tag="IVY61_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP7970048.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            5173..6036
                     /locus_tag="IVY61_000918"
     CDS             5173..6036
                     /locus_tag="IVY61_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970049.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            6067..6609
                     /locus_tag="IVY61_000919"
     CDS             6067..6609
                     /locus_tag="IVY61_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP7970050.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            6623..7045
                     /locus_tag="IVY61_000920"
     CDS             6623..7045
                     /locus_tag="IVY61_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7970051.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            6975..9374
                     /locus_tag="IVY61_000921"
     CDS             6975..9374
                     /locus_tag="IVY61_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7970052.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            9406..11397
                     /locus_tag="IVY61_000922"
     CDS             9406..11397
                     /locus_tag="IVY61_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7970053.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            11420..11671
                     /locus_tag="IVY61_000923"
     CDS             11420..11671
                     /locus_tag="IVY61_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP7970054.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            11661..12890
                     /locus_tag="IVY61_000924"
     CDS             11661..12890
                     /locus_tag="IVY61_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP7970055.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            12887..14968
                     /gene="topB"
                     /locus_tag="IVY61_000925"
     CDS             12887..14968
                     /gene="topB"
                     /locus_tag="IVY61_000925"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7970056.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            15117..19037
                     /locus_tag="IVY61_000926"
     CDS             15117..19037
                     /locus_tag="IVY61_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7970057.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            19038..19982
                     /locus_tag="IVY61_000927"
     CDS             19038..19982
                     /locus_tag="IVY61_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970058.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            19986..20189
                     /locus_tag="IVY61_000928"
     CDS             19986..20189
                     /locus_tag="IVY61_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970059.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(20489..20725)
                     /locus_tag="IVY61_000929"
     CDS             complement(20489..20725)
                     /locus_tag="IVY61_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7970060.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(20908..21279)
                     /locus_tag="IVY61_000930"
     CDS             complement(20908..21279)
                     /locus_tag="IVY61_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970061.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(21276..22604)
                     /locus_tag="IVY61_000931"
     CDS             complement(21276..22604)
                     /locus_tag="IVY61_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7970062.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(22565..22894)
                     /locus_tag="IVY61_000932"
     CDS             complement(22565..22894)
                     /locus_tag="IVY61_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7970063.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(23152..23522)
                     /locus_tag="IVY61_000933"
                     /pseudo
     CDS             complement(23152..23522)
                     /locus_tag="IVY61_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            23667..23852
                     /locus_tag="IVY61_000934"
     CDS             23667..23852
                     /locus_tag="IVY61_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7970064.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            24795..25454
                     /locus_tag="IVY61_000935"
     CDS             24795..25454
                     /locus_tag="IVY61_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7970065.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            25457..26800
                     /gene="vanS"
                     /locus_tag="IVY61_000936"
     CDS             25457..26800
                     /gene="vanS"
                     /locus_tag="IVY61_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7970066.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT         6720 a         7043 c         7280 g         5779 t
ORIGIN      
        1 attttatata aaaacaccat tttcactaat ctctaatcaa tttactgaat cgaaaggaac
       61 cgggtagaat ggataagaaa gttttaataa agaggttctc tgaagctatt gaaaaaggaa
      121 atgcagcaat atttgcaggt gcaggattat cgatgtcaca aggatatgta agttggccgg
      181 aactacttaa tgatcctgca acagaaatcg ggttggattc taaaaaagaa acagacttag
      241 ttactctggc tcaatattat aaaaatgaaa atggtggatc aagaggaatt ttgaatcaga
      301 tacttatgga taattttggt gaagaattag aaatatctga aaatcatcga atactagcaa
      361 gtttacctat tgaaacttat tggacaacta attatgatca tctaatagaa aaatcaatcc
      421 gagaagctta taaaaaccct caggttaaga aaaattatac tcagttagca acaactaatc
      481 caaatgtgga tacaattgtt tataaaatgc atggtgatat agatgatgtc agttcgacag
      541 taataacaag agatgattat gaaaaatatg atgattcgta cgcgttattt aaagaaactt
      601 taaaaggaga tttgttaaca aaaacttttc tctttttagg atttagtttt acagacccta
      661 atttagaaag aattttatca gatataagat gggttttgag agaaaaccaa agacctcatt
      721 attgtataat gagaaaaata ttatagaaaa aattttaggt tatcgctgaa agccccggaa
      781 atacgggcat ttcgcaggat atgagaactc aatttactac caatttacta ctttcggatt
      841 gagccgcata tatgctgaac catactgcta atacccgcca ccccaaatac ggcacatcat
      901 aaattgaata cgacaccgta aacgcggcgt tcctctttcc cgtcatggga gaacaggacg
      961 ccgctttttt atgcccaaat agagaaagga ggcgacgcca tgctatttac ttccggccct
     1021 gaccgggcgt ttgaacggct gatgcaggaa aagcccggtt tcgatcacta cgacggcggc
     1081 ggggcggacg cgccggagga ttgcggcacc tgccgatttt accgcccgga ttggaaatat
     1141 caattctgcc agtttgcaga gtgtccctat cagccgggca agctgaccga ctatgcagcg
     1201 gtcaaattca cagtgaaagg agcgaacaac gctatggcag ttttccgtgt ggaaaagaac
     1261 aaaggctata cggtgatgtc aaatcaccac ctgcggaata agaacctgac cttaaaggcc
     1321 aagggcctgc tgtcgcagat gttgtccttg ccggaggatt gggactatac cctaaaagga
     1381 ctgtcctata tcaaccggga aagcatcgac gcgatcagga cagcggtatg ggagcttgaa
     1441 aaagctggat atatccggcg tgagcagggc cgtgacgcaa aaggcaaaat ggccgatatg
     1501 gtctatacca tctacgaaca gccggtattg gaaaacccgg tattggaaaa tccaacatcg
     1561 gataacccgg tgttggaaaa tccgacatcg gataatccgg cgtcggaaaa tccaacgcaa
     1621 ttaaataaag aagtacaaag aactgactta tcaaaaaaag aaaaaataat tacagatgga
     1681 caaagtaccc attccattcc tatcccatcc ccgacccctg cccctatgga gggcgaggca
     1741 gcggctacgc cgccggaacg gaaaaggaca ggaaaggatg aggctgtacg gatttatcgg
     1801 gaaatcattt tggagaacat cgagtatgcc tatctcattc aggacaggag cattgaccgt
     1861 gaacagcttg acgaaatcgt agacctgatg ctggaaaccg tctgcaccgc ccgaaagaca
     1921 atccgtgtcg ccggggacga ctaccctgcc gagctggtga aatccaagta catgaagctg
     1981 accggcgaac atatccgctt tgtccttgac tgtctgcggg agaacacaac gaaagtccgc
     2041 aatatcaaac aatacctgcg ggctatgctg ttcaacgccc ccagcaccat ttccaactac
     2101 tacacggccc ttgtcgcgca cgacatggca cagcccgatt ggggaaagcc taaatccggc
     2161 ctgccggact attcctgcgg cgagggcgaa agcctgtaac gagaaaagga ggacgaccct
     2221 atgaagcagg gcgcattgat ttttgacgag cgaacagacc gttacgacat ccgctttgat
     2281 ctggccgact attacggcgg cctgcattgc ggggagtgtt tcgacgtgat gatcggcggc
     2341 aaatggaggc ccacccgcat tgaaatggcc gaaaactggt atcttgtcgg catccgggcc
     2401 gacgaccttt ccggcctgcg ggtgcggatt tgacctatat ctacctgcgg cgtctggtgg
     2461 ttccgcctta gacgccgctt ttgactttct gacaaggagg gatgcgattg caggaagaag
     2521 ttactcaaaa gaccattgcc ctgtccatga aaacgggcaa gctcacggct caagtgctgc
     2581 aagcggcgct gaaaaagtac ctgcaacacc gggccaaggg caaaaacacg ctgcaccacg
     2641 gccagcagag cctaaagcag cttaaaaagc atggggctga tctctccaac attgagatca
     2701 ccgaggccaa cattggagcg ttcaagccat gcgccaagaa atacggcgtg gattttacct
     2761 tacgaaaaga taagaccacc cagccgcccc actacgttgt gattttcaag gccaaggatg
     2821 cagataacct tgatctggcg tttcgggagt ttaccgccaa gacgctttcc aaggagcagc
     2881 ggccctctat ccgcaaggtt ttgtccgctg tgaagcaaaa ggcggcggcc cagaccaagc
     2941 agcgggccaa ggagaaaatc aaggaaaggg ggctggaact gtgaagcctg acgtaaagaa
     3001 attgctgatc ctgaacctcc cgtatctgat ttttgtgtac ctgtttgcga aatgcgggga
     3061 ggcgtaccgg ctggccgctg gtgctgacct gtcggggaag ctcctgcatt ttgcggatgg
     3121 ttttgcggcg gcttttgcca acccgctgcc aagcctgcac ctgtttgacc tgtgtatcgg
     3181 tatcgccggt gccgtgatcg tgcggctggt ggtgtactgc aagagcaaga acgtgaagaa
     3241 ataccgcaaa ggcatggagt atggcagcgc ccgctggggg acggccaaag acatagcgcc
     3301 ttacatcgac cccaagtttg aaaataatat cctgctcacc caaacggaac ggcttaccat
     3361 gaccggcagg cccaaagacc ccaagacagc gcggaataaa aacgtgctgg tgatcggtgg
     3421 cagcggttca gggaaaacga gatttttcgt gaaacccaac ctgatgcaat gctttccgac
     3481 cacggactat ccgacttcgt ttgtggtgac agatccgaaa ggcactttgg ttctggaaac
     3541 cggcaggatg ttccagcagg cgggctaccg cattaaaatc ctgaatacca ttaacttttc
     3601 caagtccatg aagtacaacc cctttgtcta catccattcg gaaaaggacg tgctaaagct
     3661 ggtaaatacc ctgatttcca acaccaaggg cgagggggaa aagtcagcgg aggatttttg
     3721 ggtgaagtcg gaacggttgt tttattcggc cctgatcggc tatatctggt acgaggcccc
     3781 agcggaggaa atgaacttct caacgctgct ggaaatgata aacgccagtg aagcccgcga
     3841 ggacgacccg gagtttcaaa gccccgtcga tctcatgttt gagcgtttgg agcagaaaga
     3901 ccccgaccat tttgcggtgc gccagtacaa gaaattcctg ctgtccgcag gcaagacccg
     3961 ttcttccatc ctgatttcat gcggtgccag acttgcgccc tttgacatcc gggaggtacg
     4021 cgagctgatg gaggacgacg aaatggagct ggacaccatc ggggacgaaa agacggtgct
     4081 gtatctcatt atgagcgata cggacacgac ctttaatttc attctggcta tgctccaaag
     4141 ccagctaatc aacctgcttt gtgaccgggc tgatgatgtg tacggcgggc ggctgcctgt
     4201 ccatgtacgc ctgattttgg atgaatttgc caacattggc caaataccca atttcgataa
     4261 actcattgcc accattcgaa gccgtgaaat atcggcctct attattttgc agagccagtc
     4321 gcagctaaaa gcgatttaca aggacgccgc cgagatcatt tcggataact gcgatagtgt
     4381 gcttttcctt agtgggcgcg gcaaaaatgc caaggagatt tccgatgcgc tggggaaaga
     4441 aacgattgac agtttcaaca ccagcgagaa ccggggaagc caaacgtccc acggattgaa
     4501 ctatcaaaaa ttaggaaagg agttgatgtc ccaagacgaa attgccgtga tggacggcgg
     4561 caagtgtatc ttgcagctac ggggtgtgcg cccgttttta tcggagaaat tcgatattac
     4621 caagcatccc cggtacaaat accttgccga cgccgacaag aagaatacct ttgacgtgga
     4681 cagatttttg actatcacgc gccggaaacg gcagcaagtg gtaacgcagg atgaagtatt
     4741 cgacctgtat gaaatcgacc tgtcggacga ggacgccgcc gctgaataaa cagcggcttt
     4801 tttcattcca acaacaaaat caaaaaatca ttatgagccg gaaaccggca gaaagaggta
     4861 aatttatggc attttttaat tccgcagtta ctactttgca gactatcgtg atcggactgg
     4921 gcggcgctct gtgcgtatgg ggcggcatca atctgttgga gggatacggg caggacaatc
     4981 cgggggcaaa atcgcagggt gtcaagcagc ttgtcgctgg cggcggcgtg gccctgatcg
     5041 gtgtcactct ggttcccctg ctgtccgggc tgctgggctg atcctggcct ttgtccctgt
     5101 ccaaaccctt aactaatcgc ctccgcgtcc tcccggccag accgggaggg cgcgggaaag
     5161 gaggtgctac ccgtgattag tgaaatcatt gaagaatgga taaaaggcat cctcatggac
     5221 ggtatcacgg gcaacctgtc cgggctgttc gatacggtaa atgccaaagt cggagaaatc
     5281 gccgccgacg tgggacgaac cccgcaggca tggaacagcg gtattttcaa tatgctgcac
     5341 agcctgtcgg aaaccgtgat cgtccccata gccggggcca tactcgccct tgtcatgtgc
     5401 tatgagctta tccagatgat aacggaaaag aacaatatgc acgactttga cagctccatg
     5461 ttcttccgct ggatattcaa atccgcgttt gcaatcctga tcgtaacgaa cacatggaac
     5521 atcgtcatgg gcgtattcga cgccacccaa agcgtcgtca atcaaagcgc cggtgtgatt
     5581 atcgggcaga catccattga ttttaacacc ctgataccag accttgaaag ccaattggag
     5641 gctatgagcg tgggcgctct tttggggctg tggttccaaa cccttgttgt gggcctcacc
     5701 atgaatatcc tttccatctg cattttcctt gtgacctatg gccgcatggt ggaaatctat
     5761 gttgtcacgt cgctggggcc tatccccctt gccacaatgg gcaacgcgga atggcgcggc
     5821 atgggccaag ggtatctgaa atccctgctg gcgctgggct tccaagcctt tttaatcatg
     5881 gttgtggtgg gtatctacgc ggtgctgata cagaatatcg gttccgcttc cgacatatcc
     5941 ggcgcaatct ggggctgtat gggctatacg gtgctgctgt gcttctgcct gttcaagacc
     6001 ggcagtatat caaaggccgt attcggggcg cattgatccc aaaatccaag catacacagg
     6061 aggaacttgt ttatttcaac gtacaacatc atttacgctg acccgccgtg gcggtatgac
     6121 tgcaaaaacg tacagggcgc agcggaaaat cactattcca ccatgagcat tgacgagctg
     6181 tgcgccctgc cggtggaacg gctggcgtcc aaagactgcc tgctgttttt gtgggccaca
     6241 ttccccatgc tgccggaagc tctgcggtta atcaaagcgt ggggcttttc attcaagacc
     6301 gtggcttttg tatggctgaa acagaaccgt aaaagcctta catggtttta cgggctgggc
     6361 cgctggacaa ggggaaacgc ggaaatctgc ctgctggcaa cacacggcaa gccgaagcgt
     6421 cgttctgcct ctgtccatca gtttattatc agcccgattg aacaacacag caaaaagccg
     6481 gatgtgaccc gtgaaaaaat cgtagagctg gcgggcgatt tgccccgcgt ggagctgttt
     6541 gccagacaga aaacccccgg ctgggacgtg tgggggaacg aggtggaatg tgaccttatc
     6601 ctgacgtgaa aggaggtgcc aaatggctta tgtaaccgtc cccaaggact taacccatgt
     6661 gaaaagcaag gttgtgtttg ggcttaccaa acggcaactg atctgttttt ccggggcgct
     6721 ggtgatcggc gtccccttat attttctcac ccgtgactat cttaccaaca gcgcggcggc
     6781 cctgctcatg gtatttgcca tgctgccggg ctttttgctt gccatgtacg agcgtcacgg
     6841 ccagcccctt gaagtggtga ttatgcagat catccagtgc tgcttcatcc agccaaagga
     6901 gcgtccctat cagaccgaca acacctacac cgcccttgtg cggcaatacc agatggaaca
     6961 ggaggtaaag gccattgtcc aaaaaggaaa tcccaagaga aaccgaaaaa acaaagctga
     7021 cccgcgccca gcgaaaagaa attgacgcgg taatccgtaa atataagggc gacggcaggc
     7081 cccacacggc gcagcagagc atcccctacg aggtcatgta cccggacggc gtgtgccggg
     7141 tatcgcccgg cgtcttttcc aagtgcattg agtttgcgga catcagctat cagcttgccc
     7201 agccggacac gcagacggct atctttgaaa aactgtgcga cctgtataac tatgtggacg
     7261 cctccattca tatccagttt tctttcctca atcggaaagt tgacccggtg cagtacgcca
     7321 agagctttga gatcgcgccg cagggggacg acttcgacga catccgggcc gagtacaccg
     7381 gcattctgca aaagcagctt gcaaacggca acaacggcat ggtaaagacc aaatacctga
     7441 cattcaccat cgaggccgaa agcgtgaaag cggcgcgagc cagattgaag cgcatcggct
     7501 ttgacctgtt aggctatttc aagagcatgg gggccgtagc ccatgtgatg gacggctggg
     7561 aacggctgaa cctgctgcat ggcgtgtacc acccggacgg cgagatattc aattttgact
     7621 ggaagtggct ggccccgtcc ggcctctcca ccaaggattt tattgccccg tcctccctgt
     7681 gcttcggcaa tgcaaagaca ttcggcatgg gcggcaaata cggcgcggta agtttcctgc
     7741 aaatcctgtc cccggagctg tcggacgata tgctggccga cttcctcaat acggaaagcg
     7801 gtgttttagt caatctccat gtgcaggcca ttgaacagac caaggcgatc aagaccatca
     7861 agcgcaaaat taccgacctt gacgctatga agattgcgga gcagaaaaag gccgtccgca
     7921 gcggttacga tatggacatt cttcccagcg accttgccac ctacggcgag gacgccaaga
     7981 agctgctgac aaagttgcag acccgcaatg aacggctgtt ccagttgacg ttccttgtcc
     8041 tcaacgtggc cgacaccaag caaaagctga ataacgacgt gttccaagcc gcaggcgtgg
     8101 cccaaaagca taactgtccc cttgtccggc tggactacca gcaggaacag ggccttgcaa
     8161 gcagcctgcc gctgggcgtc aatcagatca agatacagcg cagccttacg acttccagcg
     8221 tggccgtgtt cgtccccttt gtgacgcagg agctttttca gggcggcgcg gccatgtatt
     8281 acggcatcaa cgccaagtcc cgcaacatga ttatgctgga caggaagcag gcccgttgcc
     8341 ccaatgcctt gaagctcggc acccccggca gcggcaaatc catgagctgt aaatccgaga
     8401 tcgtcagtgt gttcttaacg acgcctgacg atatttttat ttcagacccg gaggccgaat
     8461 attaccccct tgtcaaacgg ctccacggac aggtaatccg gctgtcgcct accagcaagg
     8521 actttgtgaa cccgctggac atcaacctga attattcgga ggacgataac ccgctggccc
     8581 tgaaatccga ctttgtgctg tcgttctgtg agcttgtcat gggcggcaag aacgggctgg
     8641 aagccattga aaagaccgtg atagaccggg ctgtgcgcgt gatctaccgg ccctatctgg
     8701 cagacccccg cccggagaat atgccgatcc tgtccgacct ccacaaagcg ttgttagatc
     8761 agcatgtccc ggaggctgac cgggtggcgc aggcccttga cctgtatgtg tccggctccc
     8821 tgaacgtatt taaccaccgt accaacgtgg acatcggcaa ccggcttgtg tcctttgaca
     8881 ttaaggagct gggcaagcag ctcaaaaagc tggggatgct cattgtccaa gaccagatat
     8941 gggggcgcgt cacggccaac cgaagccaag gaaaggccac atggtatttt gccgacgagt
     9001 tccatttgct tttgaaagag gaacagacgg cggcctattc tgcggagatt tggaagcggt
     9061 ttaggaaatg gggcggcatt ccaacaggtg ccacccagaa cgtcaaagac ctgctttcct
     9121 ccccggaaat tgaaaatatc cttgaaaaca gcgacttcat tacgctgctc aatcaggcgt
     9181 ccggggatag aaaaatcctt gcggaacgct tgaatttgtc caccgaacag cagaagtaca
     9241 tcgacaattc cgaacccggc gagggccttt tgatctttga aaacgtggtg ctgcccttta
     9301 ccaaccccat cccgcacaac acccagcttt acaagattat gacgacccgc ttaaacgagg
     9361 tggcgggagt atgacccggc gacgccacaa aggaggtgaa ccaccatgaa agagctgaaa
     9421 gccaaggcga aagtgacgca ggccatgacc cgcgacgggc tgacgctgga aaatcaggcc
     9481 accggcgagg tggagaacat ttccagccgg gaggccgaac aggatttgtc cgcgccgggg
     9541 ccgggcggca ccgccgataa gctgctggaa cgggccgacg ccatccatga cggccataag
     9601 gccaagaaag ccgcgaagca ggccggggct gtggccgccg agggggacgc tgccctgcac
     9661 cggccatccg cccggctcca actgtcggag gaagaacggg ccgacccgga tttacagccc
     9721 tatatccgaa aatcggacaa acgggccgat aagctggata aggcccgcgc cgccatcccc
     9781 aagaagcgcg tcccggtcaa ggaaaccgtc tttgacgagg caagcggcaa agcaaagacc
     9841 gtcctgcact ttgacaaaca ggacaagggg cctccctccc tgaaacccaa tccggcaggc
     9901 cgtcctgttt ccgaggtttt tctattcggc cacgggaaaa tccatgaagt cgagcatgaa
     9961 aacgtgggtg tggagggcgg ccataagggg gaggaacttg ccgagcggca ggccggaaaa
    10021 gccatccgca gcaccatccg gcaccacagg ctgaaaccct actgggacgc ggaaaaggcc
    10081 gagcgcaggc tattgtctgc caacgcggag tatttctacc agaaatcttt gcgggataac
    10141 ccggagatcg cacaggcggc cagcaacccc atttcccggt tctggcagaa acagcagatc
    10201 aagcgccagt acgccaaggc cgcccggaca gcgggccaga ccgcaggcgc ggcggcacag
    10261 ggcgcggcca agacagccgg ggcctccacg aaagcggcca aggccgcagc caaagaaagt
    10321 gaacgggcgg cctcctttgt ggcccgccac tggaaagggg cgctggccgt ccttgtgttg
    10381 gggcttttga tgctgcttat catcggcggc ttacagtcct gcaccgccat gttcggcagc
    10441 gccggaagcg gcatcacggc gtcctcttac ttttccgagg atagcgatat gttggccgcc
    10501 gaggacgcct acgcggagct ggaagccgag ctgcaagact atctggacaa ctacgaggcc
    10561 acccatgact atgacgaata ccattttgac cttgacgaga tcagccatga cccctatgtg
    10621 ctgatttcca ttctgtcggc gctccatgac gggatattta ccatcgggga ggtacagggt
    10681 gatttgtcca tgctctttga caagcaatat atcctgacag aaaacgtcgt ggtagaaacc
    10741 cgttaccgca ccgaaaccga tacatggacg gacgcggagg gcaacaccca tacagacacc
    10801 taccaagtgc cgtatgacta ttacatctgc tacgtcacgc tggaaaactt cgacctgtcc
    10861 catgtgccag tctatatcat ggacgaggaa acgctaagtt tctattccct gtatatgtcc
    10921 acgctaggaa accggcctga tctgttttcc ggcagccagt accccaacgc ctccaccctg
    10981 acgccgccga cctactacga cgtacccccg gaggctttgg aggatgccac ctttgccgcc
    11041 atgctcaaag aggcggaaaa atatatcggt tatccctatg tgtggggcgg cagctctccc
    11101 agcaccagct ttgattgcag cggcttcatt tcgtgggtaa tcaatcatag tggatggaac
    11161 gtgggacggc tgggggcgca gggcctatat aacatctgca cccctgtttc cagttcgcag
    11221 gccaagcccg gcgaccttgt atttttcaag ggaacctacg acacccccgg cgtgtcccac
    11281 gtcggcctgt atgtgggcaa caatgtgatg ctgcattgcg ggaacccgat ttcctacacg
    11341 aaccttactt caaactactg gcaatcccat ttttacagtt acgggcgttt accttaacgc
    11401 cagaaaggag ctttcaatta tggcaaccaa acttgaccgc atcgaaaagg acatccagaa
    11461 aaccaagtct aagatcgcgg agtttcagaa acagcttcgg gagctggaaa cgcagaaaac
    11521 cgaacaggag aatttgcaga tcatccagct tgtgcgcggc atgaacatga agccggagga
    11581 atttgccgcg ttcttacgca gcggcgcaat gcaggccgcc cctgccgcaa ccccttacca
    11641 tgaacaggag gacaatgccc atgaagaata agaccagacg gatttcccgt attctccccg
    11701 cacttctggc cgtgatgctc tgcgtcacgg cctttcccgt taccgccctt gcaggcggca
    11761 atgacccggc accggccccg ctgccggagg ctacaaccga acccgctacg ggcggtttgg
    11821 agcctgaaac agacgaaacc ggcaccgcca cggacgggac agaaaccgag gccgaaaaag
    11881 ataccggccc tctgaccggc aaggacatca ccgacctgtt ttccgcgctg ttcggttcca
    11941 aggtgagcat tgccgccacg gacgacggca tccagatcac caccggcacc gacaaaaaag
    12001 aacctgaaca gaccggcacc gtcaccacca acggcggcag gctgaatgtc cgcaccggcg
    12061 cggggcttga caagaccgcg ttcacccagc ttcccaatgg gacgacggtt gaggtagtcg
    12121 gcacagacgg cgactggatt aaaattctct tgccggagcg catcggctat gtccattccg
    12181 actatatgac cgtcagcgaa aaggaagtag cggctaccgg cgagggcggt ttttccctct
    12241 ccattgaccc ggaggaaatt gcttctctgc tggaactgtt caatggcggc ggggccagcg
    12301 ccgccctgtc cccggacggg aacctgtcac tgattgacga catcggcagc gccgcgaaaa
    12361 atggcaagca gtttatcacc gtggaaacca aaaacggcaa tgtgttttat ctgattattg
    12421 accgggacga cgagggaaaa gaaactgtcc atttcttaaa tcaggtggat gaagccgatc
    12481 tgctgacgct gatggaggac ggggaaaccg tcacccccgc tgctgtctgc tcctgtacca
    12541 cgaaatgcaa ggccggggct gtgaacacga attgcccggt atgtaaaacc aacctgaccg
    12601 agtgcagcgg cccggagccg caggaacccc agccggagga acccgaagcc ccggaggaag
    12661 aacccaaggg cggcgcgggc ggcctgatcg tgttcctgct ggtggcgctg gcgggcggcg
    12721 gtgcggccct ttactatttc aagttcaaaa agcccaaggc tgaaaccacc ggcagcgacg
    12781 atttgggcga gtatgatttt ggggaggacg aggatttgga ggacgaggaa cccgaaaccg
    12841 agtttgaccc ggaagctgat tttgtgagtg aacaggagga cgaggaatga catataaact
    12901 tgtaatcgcg gaaaaaccca gcgtggcccg cagcattgcg ggcgtgatcg gggccacgga
    12961 gaaacacgac ggctatttgg aggggaacgg ctatctggtg agctggtgca tcggacatct
    13021 ggtgtccttt gccgacgccg gacgctatga cgagcgtttc aaaaaatggc gctatgagga
    13081 tttgcccatt ctcccggaaa catggcagta catcgtcccc gacgacaaga aacagcagtt
    13141 tgacgttctc tgttcgctga tgcagcgccc cgacgtgacc ggccttgtgt gcgccaccga
    13201 cgccgggcgc gagggggaat tgattttccg tttcgtctac cagatggcgg gatgccaaaa
    13261 gccctttgag cgcctttgga tttcctctat ggaggacagt gctattaagg acggctttgc
    13321 ccacctgaaa ccgggcggcg actatgatcc gctgtatcaa tcggccctgt gccgggccaa
    13381 ggccgactgg ctgatcggca ttaacgccac ccggcttttt tccgtcctgt accacaagaa
    13441 cctgactgtg ggccgcgtcc agacgccgac cttaaaaatg ctggtggaca gggacgccaa
    13501 gattaccgat ttcaagaaag agaaatatca catcgtccat atcacggcgg gcggcgcgga
    13561 cgcggtaagc agccgctttt ccgacgctgc cgaggcaaac accgtcaagg cggcttgtgc
    13621 aaaagcgcag gccgtttgcg tttccgtgac gcgggagaaa aagacggaac agccgcccaa
    13681 gctctatgac cttaccacct tgcagcggga ggctaaccgg cttttcggct ataccgccaa
    13741 gcagaccctt gactatgccc aggcactgta tctaaagaag ctgctgacct atccgagaac
    13801 ggatagtcaa tatcttaccg acgatatgct gcccgccgtg gaaagccttg tgtccggcct
    13861 gtgggagctg gtgccgtttg ccaagggcct gaacctttcc ccgcagtttg accgcatcct
    13921 gaacagcaaa aaggtgtccg accaccacgc cattatcccc accgctgaat ttgtgaagca
    13981 gggctttggc actctggccg agagtgaaaa aaagctgctg tccctgatct gctgcaagct
    14041 cttatgtgcc gtggccgggc cgcatatcta tgaggccgtc accgccacat ttacctgtgc
    14101 cgggcaggag tttaccgcca agggcaagac cgtcctttcc cccggctgga aagagattga
    14161 acgccggttc cgttcttcct taaagaccga cgccgacgag gacgccgagg cggtgcggga
    14221 gctgccggag ctttgcgagg gccagacctt tgcagacgtg gccgcctctg tcaccgagca
    14281 tttcaccacg ccgcccaagc cctacaccga agatacgctt ttgtccgcta tggaacgggc
    14341 cggggccgag gatatgccgg aggatgccga gcgcaaggga ttaggcacac cggccacccg
    14401 cgccgccatt ctggaaaagc tggtgcagat gggctttgtc cagagaaaag gcaagcagct
    14461 cattcccacc aaggacggca tcaatctggc tgtggtgctg ccggaggcgc ttacttctcc
    14521 ccagcttact gccgaatggg aaaaccggct gaccgagatc gccaaggggc aggccgatcc
    14581 tgacgaattt atggacggca tcgagacgca ggcgcgggag ctggtgcaga cctattcctg
    14641 tatcagtgag gaaaagcaga aattgtttca ggccgagcgt gttcctatcg ggacgtgtcc
    14701 ccgctgtggt gaggctgtct atgagggcaa gaaaaactac tactgcggaa accgggcctg
    14761 ccagttcgtc atgtggaaga atgaccgatt ttttgaggaa cgcaagaaag cgttcacccc
    14821 gaagatcgcc gctgcgctgc tcaaaaccgg caaagtcaag gtaaagggcc tctattccgt
    14881 aaagaccggc aaaacctacg acgggacggt gcttttggct gataccgggg gcaagtacgt
    14941 caactaccgc attgaacaca ggagctaaag gacagtaaca cagcaagcat agggagtgtg
    15001 gctacactcc cggtaaaatc ccccggccgc gtccctgccg ggccggggat ttttgcttgt
    15061 tggggaagtc cccaaccccc tattttttga aacagaacag agaaaggagt gaccgcgtga
    15121 acgaagtatt ttctatccaa gtccataacc ggcagttttt ggaacagggc aaacaaggcg
    15181 tctggctgga tttgcccacc acaaccgaaa cgctgcaagc cgccctgcgg gatgttggca
    15241 ttactgccga caatccgcag gattttttta tcaacgggta ttcctgcccg gaggacagac
    15301 accttgccat tccctatgac atggtgctgg ccgccgacgt ggacgagctg aatttccttg
    15361 ccgcccggct gggccagctt gacgccgccg agatcgcaga gctgaacgcc gccctgcaaa
    15421 atccccgtgg cggctttgag aatatcggcc agatcatcga ctacaccgaa aatgtggatt
    15481 actatgtgca tcttcccgac gttagggggg atggacagtt gggcgactac tacctgaacc
    15541 gttccggcat ggtggatatg ccggaggaat ggaaagccgg gattgatacg gcgcagtttg
    15601 gacgccacat tgcccagcag gaacaaggcg tttttaccca gtacggctat ctggtgagaa
    15661 gcggcgacga atggcagcgc gtccatgagg gccagccggt gccggaggaa taccgggtga
    15721 tgcactaccc ccagccggaa gctgaccggg aggaaccgca ggcccagcag ccggaggcat
    15781 ccgaaagccc cgacaaagcg ccgcccccgg ttatcccgat catgctggac ggcatgaaca
    15841 gcaaagaacg catgaaagtg attactgacc gtctggaaac cggcgtacag gagctttttg
    15901 aaagtagccg ctatatggct tacttatccg ctatgtccaa gatgcacgat tacagtctga
    15961 acaatacgct gctgatcgcc atgcagggcg gttcgattgt ggcgggctat aacaggtggc
    16021 aggatgaatt tgaccgccat gtgaagcggg gcgagaaagg cattaagata ctggccccgg
    16081 ccccgttcaa ggttaagcag cagatggaga aaaccgaccc ggccacccag cagcccgtga
    16141 tcggcgcgga cggcaagccc gtgaccgaag aacaggaagt gacggttccg gcctttaagg
    16201 tggtgacagt ctttgacatc agccagaccg agggcaagga ttttcccatg ccctatatca
    16261 gtgaacttac cggcgacgtg cagcagtatc aggatttctt tgccgcgctg gaacggacat
    16321 caccctatcc cataagcatt gagccgattt cagacggggc caagggccgc tgcttctacg
    16381 caaaacagcg cattgccatc aacgagggca tgagcgaatt gcagacccta aagaccgcca
    16441 tccatgagat tgcccatgcc aagctccacg ccattgaccg ggaggccccc gccgaggaac
    16501 aggcggcccg gccagaccgc agcacccgcg aggtacaggc cgaaagcgtg gcctataccg
    16561 tctgccagta ttacgggctg gacacgtcgg actattcttt tggctatgtg gccgattgga
    16621 gcagcggcag ggagttatcc gagctgaaag cctcccttga aaccatccgg gccacggcca
    16681 gcgaattgat aaccgagatc gacggccatt ttgcccagct tcagcaggag cgcgaggccc
    16741 ggcaggaaca ggaggccgtc acggttgacg ttaccccgga ggcagcggcc ccggcgcagg
    16801 ccgaggaaga acaggccccg gcccttgatc cggcagccga gccgcttgtg actgtgctat
    16861 ggagcgaaag cccccagcta aaggacggcc agcaaatgcc gttgcatgag gccgacgcgc
    16921 tgtttgcccg tctggatgca gcatatccgg cagaggcagg ctatgacaaa accgcgttcc
    16981 gcattgactg tatctttcag ggcgagccgg acagctacga ggggcggcag gattttggag
    17041 atagggacgg ttccctgatc gaacatatcc agagctacca ccaattttac gcgcaggacg
    17101 aaaactggaa aaagcatgta atcgctacaa ccggcataga gggctgggag caggacaagg
    17161 cccatcggga tatgctgtta ggcgagtttg tcccctatat gaagctgcat tgcagccttg
    17221 cccaaatgga acagacggcg cagggccgct tacagtccgg ggaaaccctg acgccggagg
    17281 aaacggccta tttgggggcg cttctctctt atgtgcagga gtgccgcccc atgctcaatc
    17341 agggccagta tcagttgccg gagccgccga aactggctga cttcgaccag agcttgcagg
    17401 actacaaagc acaggtacag gccgagatcg cgcaggaggc cgccgcagcc ggtatgaccg
    17461 tggaggaata cgcggcggct ggctatgaag caccccaaca ggacaccttt tccatctacc
    17521 agcttcgagg cggggaggaa acccgcgact atcggtttga gccatacgag cgtttacagg
    17581 ccgcagggct ggccgtggac agtgccaact atgaccttgt ttatacggca ccgctggata
    17641 aaagcacgtc tttggaggat atttaccgca cattcaacat cgaccacccg gcagatttta
    17701 ggggacacag cctttccgtt tccgacgtgg tggtgctgca ccagaatgga caggacaccg
    17761 cccactatgt agacagcttc ggctatatgg aagtgccgga gtttttgcgg gaaaatcccc
    17821 tgcgtaccgc cgaactgtct acggagcaga acgaaaacat gattgacggc attatcaaca
    17881 atacgccgcc cgaaccgcag ccggaggaac ccgaacagct atcttttgag gatgtggcag
    17941 ttgcttccgg ccagacacag gaggaacccc cggaggctgc gcccccgcaa acggctgtcc
    18001 gctattatcc catcaatgag ggagccgccc gccgcgccaa ggaagccatg agcttttctg
    18061 actaccggcc cggcagcgcc acggcggaat accggcacta tgtcgatcag gccgtggaaa
    18121 ttgcggagcg ccagaaaaag cgtgtggagc cagagtatca cgaaaagata gacagcctcc
    18181 ttgacaccta cgcccgcaag ctggccgaga acatgaataa gggctatgag attaccgccc
    18241 gcgtcccctc tgtcctgatt gccgggccgt ccaatttccc ggtacgggcg aaagaaaagc
    18301 agaacgccgc cagtgaccgc aacatggagg aatttcagta cattcagggc ttgcttgaca
    18361 aaatccgcag caccggcatg ggtggtatca gcgccgacga tccgcaggcc gtttccaagc
    18421 tggaaaagaa actggaaaag ctggaagcgt cgcaggagtt gatgaaggcc gtcaatgcct
    18481 actaccgcaa gcatgggacg ctggacggat gcccccatct gatcgaaaga ggcattgaaa
    18541 acctgaaagc ggatatggcg tcgggctggc actatgagaa aaaaccgttc cagtcctggc
    18601 agctctccaa caataacgcc gagatacgcc ggttaaaggg ccgcattgag gaattgaccc
    18661 gccaaaagga ggcggcctat gtgggatggg agtttgacgg cggcactgtg gagatcaacc
    18721 gggtggccaa ccgcctgcaa atcttctttg aggggaagcc ggatgctgct gtacgcgacg
    18781 agctgaaaag caacggtttc cgctggtcgc ccaaggccgg ggcatggcag cggcagttaa
    18841 acgacaccac aatccgcgtc gctgaccgta tcaagtgcat ccagcccctt tccggcgaaa
    18901 agcccagcgc cttacagctt gcggcccgca gggagaaaga aaagccctct atccgggcgc
    18961 agctcaacgc ggccaagacc gaacaggcca agcagccacc ggcgcgggaa aaatcaaagg
    19021 gattggaggt agagtaaatg cagaagtttg aaaatgtgga cgttctggcc gcgctggaac
    19081 agatcatgcg gcagaacacc gcgttttatc aaagtgactt tggcattgac aaagacatta
    19141 tcaggcggga tgccgccagc gatcaggccg cagacaagac cctgctctgg atgtcccggc
    19201 cctccgggac gtactgtttc cgggaacgtg atgttttcct gaaagacacc cgacagtaca
    19261 acacatggaa attttacggg gagcagaccc gtgacaagat actggcctac gcggtggagc
    19321 tgaccggcac acagggcggc actatccggg gcaatctcta tgagctggac tatccgcagc
    19381 atttccggca cgtcattgaa accgcccagc cggtgagtgt gaaccggctc ttttatgagc
    19441 atggcacccg tgacatcccg gagagccagc gttttgacgg aagccctgac cgggtattgg
    19501 gaaacttttt gtactatgag gcccagcccc atgacccggc tgtgctgcag gaggcgctac
    19561 ggacggagca gcacagccgg gggcgggccg tccccggcga tttcaaagtc cacgtcaccg
    19621 ctttgcatga cagccggatt gaaaccgagg cccggcgtat tgtggacagc ctgaaagatc
    19681 tgtccgcgcc taacagcccc aataaaacgc atttcatggt ggagatctcc ccgcactttg
    19741 cggcgattgc ttccaccaag gataatgacc ggctctttgc catgctgccc tataaatccc
    19801 tgtgttttac cgggatgaaa ggcaggcatg gcgtgtatgc cgtgattaac cgggacgagc
    19861 gccgggatgt gagtattcgc aggccccggc cctctatccg caagcagcta tccgacagca
    19921 aacaggccgc cagcccaaaa aaggcggcgg ccagaaccaa aaagaatgaa ttggaggtat
    19981 aaggaatgat gcactttacc gtggaggaag aaaacctgat ctgtatgtac cacaatgcag
    20041 accggcgcag aacgataaac aggctcaccg ccgtcatgcc ggttatggat ggggatatgc
    20101 gggcgcaggc ccagcagacc ctttccaagc tggaacgcat gacggatgcc gactatgacg
    20161 gccagaagtt ccacttcaca gacgaataac gaacagcaga cgccgggcgc ggcggccatg
    20221 tggtcgcttg cgtccggcgt cttttctttt tgcctattcc tacgcattta gaacccggta
    20281 ttgtcgggga taggatgaag tattcgcttg cccctgcaaa catgcgggag aagccttgca
    20341 gcgggcggtt ttcaatccct taaatgcgta ggttgcagct ataattagac ccctgaaaca
    20401 gatatagccg cgcagcgggt gtatctgttt caggggtgtg caggtgatag ccgcaaagcg
    20461 gcgtaagagg gtgcagccct cttgccgtct atctctgttc cagcgggaag ctcggcaatg
    20521 cttccagcag tttcagggaa atgcgctgcc gtatatcttc gtcaatgact ttcttctttt
    20581 tgccgtttgc ctgtctgatc tccaccgtgg aaagctcgtt gatgtagtca gcataatggg
    20641 ccaacacttt ttccgtggcc cacctctcac cggcaacggc ggctttgatc gtttcatagt
    20701 ccagcagctt ttcttgtccg tccatcctga tacccccgtc agactgattt tttctatgat
    20761 accatattcg gaagcatgga accagagaaa acggcgcttt tctgctggta aattctacgc
    20821 atttagaaca gcacttcggc ggggatagga taaaacaccc acataccccg ctgaccatgc
    20881 cggaaaagcc ttgaaatata agggctttta cagctctaag tgcgtaggtt tccagtatct
    20941 tttccgctgg cgctcggcct ctttgcggcg gcgcatccgt ttggagcagg ccgcgcagta
    21001 tttggcccgg ttagacttcg gcgtgaacgt ggccccgcac tcacagcagc gtttcacttc
    21061 gtcccggtct ttggtgatct cggcatacag ggccgcatcc agcgggagga cagcggcctt
    21121 aaaccagcgg cacagaagcg aataggaaat gctctgtacg caaatacatt cttcgccgtc
    21181 gtccagcaac aggcagttgc cgtcacaata gttgcagcac tcatgcgtca gcttccgggc
    21241 cttgcgatat tgagggtaat tcattcgtgg aatactcata gctcttgcgt ccattccttt
    21301 cccggcgtca tacgcaaaat gccgtccaca ttctgcttga tgatgccgta ttcctgcatc
    21361 tgctttttcg cttcgccgta cttctcatac agccgttcct tttcctcggc caatcgcttg
    21421 tactcggctt tcagggcgta cagatcgggg agctttttca caccctgtcc ctttagggcc
    21481 tttgccgcag cttcatacaa aatcaacgtg ctttcatgct cacgccggaa ttttgcctta
    21541 tccgctgcat tgcggtattc catagccacg ggccagagct gcttgtaggt agaaatattc
    21601 tttatcagca ccgccatatc cgcaagccgg tgttccatag ctttgagggt agcggcggcc
    21661 tcgtcgctgg cggcggtgat ctcggccact ttgctttcca aatccgggta ggtgtctatc
    21721 ccatgttcag tcaggaaatt caaagtcttt gcggcctgct tcaaattgtg gagcttcgcc
    21781 cacctctcat agcctgccga ctgctgggcc ttaatgttgt tttccagatc aatccgcaaa
    21841 gaaatcccct tgcgctcttt gggcgctctg gtgataacgc ggcctttgat acgctctttg
    21901 atggcttcct cggtgtaggc ttcccccaat gtcttgcagc gggtaaagcg ttcctgtccg
    21961 ggagcgcgga aagaaataaa ctttcccggc ttgatctcat agccctgcgc ctgcatgagc
    22021 cgcaggaaat catcaaaatc ttttgcctgc gggatagcgg cgtcaatggc tgctttgagc
    22081 ttcgctttcc agctcgtccc tttccgctgg gcgtcccact cggcatagct cttgccctta
    22141 ctctggccgg gcttcaccac ggacaatccg ttttccttgc acagcctgtc gctggtgcgc
    22201 cggatatagg catagctctg tttgttggaa atgtacttgc gctgatttac catatccacg
    22261 gcacagaaaa taatgtgatt gtggacgtgg cccttatcaa tatgggtagt cagcacatag
    22321 ggatatttcc cttgcagcac ttcctcggca agctgcttcc cgatctcatg ggcctgttcc
    22381 ggcgtggctt cgccgggggc aaaggattgt atcaggtggt ggcccagatt attgcccttt
    22441 tggtatgcct gcgccagcag catttcaaat tcaatgtccg ctgtttcata agagcaggca
    22501 aaggaggcca ccagcagctt gccgtcagtt ttgtccgggt tctcgatgta gtcaagggct
    22561 tttttcagcg ttgacttgat aggatgtatc tttgtaactg ccatatctgc gccagcgccc
    22621 cctttatatc ctcgatgtcc tgtgcgtaca ccggccccat accgttcagc ctctttgcaa
    22681 tctgattgac gttgacgccg atcttctgta tctcggcggt ctgcgcccgg atgtcgctgg
    22741 tgtccatgtt gacgatatag ccgtcaatga gcatcttccg ggcgtaggct gaaaagttct
    22801 tcgtatgcag cagcgccatt ttctgttcaa tcatagcccg ttcctccggc gtcacaggga
    22861 cgcgcagcac gatttttcgc tttctgtttg ccatgcttca ccgtcctttc cattcagggg
    22921 tttggggatt tccccaacaa gcaaaacgga cgcggcccgg cagggacggg gcaggccgtt
    22981 tttccgggag tggctacact ccctatgctt gctacgttac tgtttctcca ctaacaagac
    23041 gtttccaaag ccccaaacta cccgcaaaat agccctctgg aaagaaaaaa gtgcaaaaaa
    23101 atagaagcta cgctgtgcag ctcccgctaa aagtaaaaca gaggatgcca gttacacatc
    23161 ctccgtttca ttcttcttcg gcttttcaat tagtatggcg ctgtcaatcg cgccctcgat
    23221 gatcggcagg tattcttcgg ggcagagttt cagcttgtgg ctgacccttt gccgcagttc
    23281 gctttcctcc cgcatgatct ccgggttaaa gtagcgttcc acaggaagcc cgcaggcttt
    23341 aataagctgt atgacaaccg gcagactggg aatggcccgt cgttttcgat gttggcaaga
    23401 tagcgccagt caatatttat tttctctgcc attgctttgc gcgaaacgcg ctttgcttgc
    23461 cgggccgctt tcacatccgc gccgaaagtt tcaaagccgg gacaatcttc aatattcgcc
    23521 atatcgcacc tcccacttac attgtatatt tcatactctg cccgtggaat gttggtatat
    23581 gcacctttct taaagtttat atttcacatt ttgtttcttt tattcttcga gcaaaagaat
    23641 tataatgtac cctgtggagg tgtactatgg actacatgac gttgaaagag gcaggcgaga
    23701 aatggggtgt gcccccacga tggataaact actattgtgc tgcgggacgc atccccggcg
    23761 ctgtaaagat ggcgacgatc tggctcattc ccaaaactgc ggaaaagccg attgacaggc
    23821 gtacaaagaa aggaaaggcg gttactgaat agtataatga aaccggcgca ttttggatat
    23881 ggttttgaag agagagaaag catggtattt tgtcacggaa aaactataag atgatagatg
    23941 gcaggctgct ccagaccgat aaaaagtatt ctcatctgaa acagaaagaa aagatttaca
    24001 catggatgta tgaagaagcg aaactttatc atgacagaaa tggaaaatgc ccgaaacaaa
    24061 gacgaggaag ctgctgctgt atcagccgta tatggtagaa ttgaaaaggc cggcatctgg
    24121 attctttatg gtgaagtctc cagacattgg ataactgctg gtctaatgcg cctttgagta
    24181 tggggtgatt gattttaata ccggggaggt acggcttttt gaggaaaata ttctgggatt
    24241 tacatttggc gattattaca tttcctgtaa ctgcaaggac acggatgatg aagatatgag
    24301 gaatatagac ttgctgattt tctactgtct tgaacggtat taggactgaa tctgctgcgc
    24361 ggagtgcggc aagagcgacc ggagggagta cacacaggcc ctgcctgtgt gaaaagccat
    24421 accgcaaggt gcattcgcaa aaaagaggcc gcgcattttg ctgaggcttg gggtgtcccc
    24481 aacaagcaaa acgagaacgc cgcaggcggg caagtggtgg atcagaatat ccgtaaagga
    24541 tattctgatc cattgcttgc cagtattatc gggatgtttt gacaaccgct gtaatcgaaa
    24601 catcccaaac attaagaagg tgttaattat tattatcttg atttaattcg tttaaaccaa
    24661 taatatagat acatacacca tgaaaagaag cgagtatccc aatgacggat gattaaaatc
    24721 cgaaaagaaa tcgctttgaa actacaggga aactacagac tgttatgtta tcttcttaaa
    24781 atggagggat ttttatgtcg atacgaattc tacttgtcga ggatgatgat catatctgta
    24841 atacagtaag ggcgtttctg gctgaggcag gatatcaggt ggatgcctgc acagatggaa
    24901 atgaggcata caccaagttt tacgaaaaca cttatcaact ggttattctt gatattatgc
    24961 tgcccggtat gaacgggcat gaacttttgc gtgaatttcg tgcgaaaaat gatactccca
    25021 ttctgatgat gacagccctg tcggatgacg aaaaccaaat ccgggcgttt gatgcagagg
    25081 cagacgacta tgtaacaaag ccattcaaga tgcagatttt actaaagcgg gtggaagccc
    25141 tgctgcggcg cagcggtgcg ctggcaaagg aaatccgtgt cggcaggctg acacttctgc
    25201 cggaggattt cacggtactt tgtgacggta cagagctgcc cctgacacga aaagaatttg
    25261 aaatcctttt gctgctggtg cagaacaaag gcagaacctt aacgcatgaa atcattttgt
    25321 cacgtatatg gggatatgac tttgagggtg atggcagcac agtccacact catatcaaaa
    25381 atttgcgggc caagctgccg gaaaatatca tcaaaaccat tcgcggtgta ggataccgat
    25441 tggaggaatc ataataatgg aaagaaaagg gattttcatt aaggtttttt cctatacgat
    25501 cattgtcctg ctactgcttg tcggtgtaac agcaacactg tttgcacagc aatttgtgtc
    25561 ttatttcaga gtgatggaat tacagcaaac agtaaaatcc tatcagccat tggtggaact
    25621 gattcagaat agcgataggc ttgatattca agaggtggca gggctgtttc actacaataa
    25681 ccaatccttt gagttttata ttgaagataa agagggaagc gtactctatg ccacaccaaa
    25741 tgccaataca tcaaatagtt ttagacccaa ctttctttat gtggtacata gagatgataa
    25801 tatttcgatt gttgctcaaa gcaaggcagg tgtgggattg ctttatcaag ggctgacaat
    25861 tcggggaatt gttatgattg cgataatggt tgtattcagc cttttatgcg cgtatatctt
    25921 tgcgcggcaa atgacaacgc cgatcaaagc cttagcggac agtgcgaata aaatggcaaa
    25981 cctgaaagat gtaccgccgc cgctggagcg aaaggatgag cttggcgcac tggctcatga
    26041 tatgcattcc atgtatgtca ggctgaaaga aaccatcgca aggctggagg atgaaatcgc
    26101 aagggaacat gagttggagg aaacacagcg atatttcttt gcggcagctt ctcatgagct
    26161 aaaaacgccc atcgcggcta caagcgttct gttggaggga atgcttgaaa atatcggtga
    26221 ctacaaagat cattctaagt atctgcgcga atgcatcaaa atgatggata ggcagggcaa
    26281 aatcatttcc gaaatactgg agcttgtcag cctgaatgat gggagaatcg tacccatagc
    26341 tgaaccgttg gacatagggc gcacggttgc cgagttgctg cccgattttc aaaccttggc
    26401 agaggcaaac aaccagcggt tcgtcacaga tattccagcc gggcaaattg tcctgtccga
    26461 tccgaggctg ctccaaaagg cactatccaa tgtcatattg aatgcagttc agaacacgcc
    26521 gcagggaggc gaggtacgga tatggagtga gcctggtgct gaaaaatgcc gcctttttgt
    26581 tttgaacatg ggcgttcaca ttgatgatac tgcgcttcca aggctgttca ccccattcta
    26641 tcgcattgat caggcgcgaa gcagaaaaag tgggcgaagc ggtttaggac ttgccatcgt
    26701 acaaaaaacg ctggatgcca tgagcctcca gtatgcgctg gaaaacacct cggatggcgt
    26761 tttgttctgg ctggatttac cgctcacatc aacattgtaa atatttaaaa cttaaatgat
    26821 tt
//
LOCUS       DACCXM010000026        26766 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCXM010000026 DACCXM010000000
VERSION     DACCXM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.028"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..1957)
                     /locus_tag="IVY61_000937"
     CDS             complement(26..1957)
                     /locus_tag="IVY61_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7970067.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="IVY61_000938"
     CDS             complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="IVY61_000938"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7970068.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2886..3638)
                     /locus_tag="IVY61_000939"
     CDS             complement(2886..3638)
                     /locus_tag="IVY61_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7970069.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3797..4006)
                     /locus_tag="IVY61_000940"
     CDS             complement(3797..4006)
                     /locus_tag="IVY61_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970070.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4017..5344)
                     /locus_tag="IVY61_000941"
                     /pseudo
     CDS             complement(4017..5344)
                     /locus_tag="IVY61_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5709..6296)
                     /locus_tag="IVY61_000942"
     CDS             complement(5709..6296)
                     /locus_tag="IVY61_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP7970071.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6379..7629)
                     /gene="clpX"
                     /locus_tag="IVY61_000943"
     CDS             complement(6379..7629)
                     /gene="clpX"
                     /locus_tag="IVY61_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP7970072.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7876..9165)
                     /locus_tag="IVY61_000944"
     CDS             complement(7876..9165)
                     /locus_tag="IVY61_000944"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP7970073.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9319..10251)
                     /locus_tag="IVY61_000945"
     CDS             complement(9319..10251)
                     /locus_tag="IVY61_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970074.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10408..11067)
                     /locus_tag="IVY61_000946"
     CDS             complement(10408..11067)
                     /locus_tag="IVY61_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7970075.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11070..12095)
                     /gene="sppA"
                     /locus_tag="IVY61_000947"
     CDS             complement(11070..12095)
                     /gene="sppA"
                     /locus_tag="IVY61_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7970076.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12108..12398)
                     /locus_tag="IVY61_000948"
     CDS             complement(12108..12398)
                     /locus_tag="IVY61_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970077.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12807..14612
                     /gene="glmS"
                     /locus_tag="IVY61_000949"
     CDS             12807..14612
                     /gene="glmS"
                     /locus_tag="IVY61_000949"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP7970078.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14684..16039)
                     /locus_tag="IVY61_000950"
     CDS             complement(14684..16039)
                     /locus_tag="IVY61_000950"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP7970079.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16063..17232)
                     /locus_tag="IVY61_000951"
     CDS             complement(16063..17232)
                     /locus_tag="IVY61_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970080.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17229..18116)
                     /locus_tag="IVY61_000952"
     CDS             complement(17229..18116)
                     /locus_tag="IVY61_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP7970081.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18305..21997)
                     /gene="nifJ"
                     /locus_tag="IVY61_000953"
     CDS             complement(18305..21997)
                     /gene="nifJ"
                     /locus_tag="IVY61_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP7970082.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22196..22717
                     /locus_tag="IVY61_000954"
     CDS             22196..22717
                     /locus_tag="IVY61_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7970083.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(23416..23973)
                     /locus_tag="IVY61_000955"
     CDS             complement(23416..23973)
                     /locus_tag="IVY61_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970084.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(24152..24763)
                     /locus_tag="IVY61_000956"
     CDS             complement(24152..24763)
                     /locus_tag="IVY61_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970085.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(24876..25133)
                     /locus_tag="IVY61_000957"
     CDS             complement(24876..25133)
                     /locus_tag="IVY61_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970086.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(25730..26008)
                     /locus_tag="IVY61_000958"
     CDS             complement(25730..26008)
                     /locus_tag="IVY61_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970087.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(26042..26293)
                     /locus_tag="IVY61_000959"
     CDS             complement(26042..26293)
                     /locus_tag="IVY61_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970088.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         7557 a         5767 c         4584 g         8858 t
ORIGIN      
        1 aaacccagcc tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata
       61 aaccataaat cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg
      121 ggtgactcag taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga
      181 aagctccgac taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa
      241 tcgggtcagc tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga
      301 tgttcgtcaa accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag
      361 caacgaaaat tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac
      421 ttccgccagt tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt
      481 tgattggacc gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag
      541 cattcgaacc actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag
      601 ggacattgac cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga
      661 ggatcgtttt aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata
      721 cgatgacata accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg
      781 tattagctaa tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg
      841 aagcaataaa tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt
      901 tgaaccagct agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc
      961 caataaattg gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag
     1021 gtaacatgtg tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat
     1081 ccgcatttcc ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac
     1141 cactaagggc tagattgatc aactcttctg gtttacggat accatcactg acaggacgat
     1201 tgatcagttc ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta
     1261 caccttctgc acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt
     1321 tcgtttcgac tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg
     1381 ccatgtaagt gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt
     1441 ctggatgagc agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt
     1501 tttgctcttc agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta
     1561 attttcctac gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat
     1621 gtgtgtcgtt tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt
     1681 ctaaggcttc ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa
     1741 ctgcactgtt tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg
     1801 cttcgcgggc tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa
     1861 acattttttg gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga
     1921 tcatcgcttc tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc
     1981 aatttttgtg atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc
     2041 atcagaaaaa gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc
     2101 tttcgtacgg ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga
     2161 atttttcaat gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa
     2221 agcaccgtct ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt
     2281 cccatacgga atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc
     2341 gtgattgttt ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc
     2401 tgttgtatca atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata
     2461 aaatcctttt cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc
     2521 tactgttcct aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa
     2581 gccattgatc tcagtttcag tgtctgattt tagtttgata ttgattcttg tatcttcttt
     2641 tacggttgtg aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc
     2701 agtgaaccct cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg
     2761 ggaaacattg atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc
     2821 gcctagtttt aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt
     2881 gtaaatcatt gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct
     2941 aatggacaac gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac
     3001 aactggttga atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct
     3061 aaacgtttaa cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca
     3121 ccgttcattc ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta
     3181 gatcctaaaa cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc
     3241 tccaaatcaa ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt
     3301 ccttgcagaa aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct
     3361 tcgtcattaa tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg
     3421 agtgattttt cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca
     3481 tgaactcttt ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat
     3541 tcagaagcat tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc
     3601 aagtctagga ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa
     3661 ataatagcat attttttttc tactagcaat ataaatcagt caaaaacgtt cataaaaatt
     3721 ataaatcagt caaaaaaaaa taaaaaacca gtcagaaaga ctggtttttt atttttttgt
     3781 ctatcatcaa gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag
     3841 aagcaggttt tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga
     3901 ccaaaaaacc gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata
     3961 ataatgatgc cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat
     4021 attttttgta aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga
     4081 tgctgctcac ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg
     4141 aaggtaacat cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct
     4201 gaaggaacat agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct
     4261 acttctagat attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca
     4321 gtcggatcga taactttata gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa
     4381 tacgcctgag catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttggata
     4441 tcataacttt ttgttacaaa aaatgaagca ttctttttag gaacaggttg catttgtgca
     4501 ttctgatact ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt
     4561 ttcaataaac cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa
     4621 taggattgaa acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg
     4681 atacatatga cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa
     4741 atcggtttag ggatgatttg atgataatag cgactataat acatttccca acacgaagtg
     4801 aaacataaac cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact
     4861 tccccgtttt gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat
     4921 agccgttgag agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag
     4981 gcgaacaccc catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt
     5041 tctttgaaaa cagagataga caatacattt tttatcaata attgattatc ttccaagcga
     5101 aaactacatt cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct
     5161 agcattattt tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag
     5221 cggataaatg ttggctggat caacagacgt gtcaaaatag cccaatcgct tatttcctgt
     5281 ttttttcttc ttcatttaag tctgtgagct ggatagtcat caataattta tcttcgccaa
     5341 tcattttctc acctctttca aatcttttta taatttaatt ataaccaatg ataaagaaaa
     5401 actaaaactt tatgtcttag aaaccaattt ggacgattac atgatagact aagtaaaaag
     5461 aaacaaagag gtgtttacaa tggggttgaa atttgaacgt acagataatt tagataaact
     5521 tttcgaatca tttgctgtcg atccggaaaa aacagttgca ccaaaagaag agatcgatcg
     5581 atttctaaaa ccacagccac aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa
     5641 gctcaaaaaa agacttacca gccaaacttt tatgaaagag tttgactggt aagtcttttt
     5701 tattcttttt atgattcaat tgcttgttcg attgctgccc acgcttcttc ttttccctgt
     5761 tttgtaacgg aagaaaagag aataaacgtg tcattgcgat caaaatccaa cgcttttttg
     5821 atttcgctct catgcttgtt ccatttacca cgagggatct tatctgcctt agtggcaaca
     5881 acaattacag ggatatcata atacttcaaa aattgataca tctggatatc ttcttgagaa
     5941 ggcttatgac gcaaatctac taaagaaacg accgcacgta gctggtctct agtcgttaaa
     6001 tatgtctcga tcatctttcc ccattttgct cgctcagttt tggatacttt tgcataccca
     6061 tatcccggaa catcgacaaa atgtaatgcg ttctcgatca aataaaaatt caatgtctgg
     6121 gtctttcctg gcttaccgga agtacgggct aaattctttc gatcaatcaa tgtgttgata
     6181 aaagaagatt tccctacatt tgatcgtcct gccagagcta tttctggcaa ttgtgtatct
     6241 ggatattgtt ttggtgccac tgcacttatg ataatttcag cttgatggac tttcatggtc
     6301 tctctcctta acaaggtgat gaataaagca agcggctgtc gcaaaagcca aaatgacttt
     6361 tacggcagcc tcttttcatc aacccgcttt tttctctgaa tctttataga ttactgttgg
     6421 ctcaccagac aattcagctg cttcttttgt gataatcact ttttcgatgt tttcatttga
     6481 agggatatca aacatcacat ccatcatgat ttcttcaatg atggaacgaa gtcctcgagc
     6541 accggtattg cgttcgattg cttttttcgc aatggctctc aacgcttcag gttcgaattc
     6601 aagttcggta ttatctagag aaagaagttt ttgatattgt ttgactagcg catttttcgg
     6661 ctcagtcaag atccgtacca gatcatctgt tgtcaatttc tctaaagctg ccataacagg
     6721 taatcgtccg ataaactcag ggatcaatcc gaattttagt aaatcttcag gaatgatatg
     6781 ctgcatgaca ctttcgtctt cagaaagttt ttggttttgt gtaccaaatc caatcgtctt
     6841 ttcgcccata cgattcttca caatcgtttc gatgccatca aatgcaccac ctacgatgaa
     6901 caatatgttc gtcgtatcaa tctgaataaa ttcttgatgt ggatgtttac gtccgccctg
     6961 aggtggcaca cttgcaacag ttccttctag aattttcagc aaggcttgtt ggacaccttc
     7021 gccggaaacg tcacgagtaa tagatacatt ttcgctttta cgcgcaattt tatcgatctc
     7081 atcaatatag atgatccctt tttctgcaag ttccacattg taatcagcag attgaagcaa
     7141 tttaagcaaa atgttttcta catcttcccc cacataacca gcttctgtca aacttgtcgc
     7201 atctgcgatt gcaaaaggta cattcaatgt ttttgctaat gtttgtgcta aaaatgtttt
     7261 accagaccct gtaggtccga tcaagcagat attgcttttt tgcagttcaa catcatctgt
     7321 gttttcttgt gcttgctgat tgacacgttt atagtgattg tatacggcaa cagctaacga
     7381 gcgttttgcg cgatcttgtc caatcacgta gttattcaat acttcaagaa tttctagtgg
     7441 tttagggact tcagtgaatt cgcgtacagc ttcttcataa aattcttcat caatgatttc
     7501 tttacatagg tcgatacatt cattacagat gtagacccct ggaccagcta cgatcttttt
     7561 cacttcttct tgggttttcc cgcaaaaaga acagcgtacg gtctcattgc tacttggatt
     7621 gtcatacatg gtcttcaccc cttaacttgc aaaaagtatg caagaaacta tcaaagactt
     7681 tacagtcttt tactatcata acatagttta gtaaaaagaa aaagtgttta acaaaggatc
     7741 aactgaccta tacctttgca ttttttactc ttaacaaaaa ataaggaact gtgacaaaat
     7801 cgtgtcacag tcccttattt cgaataaaca attttctaga agtagcttct tcagaaatga
     7861 tttattactt gatttctatt ctttcgcttc tgtcgctgta cctgtgatca attcgattgc
     7921 ttttttcatt gtcacatcat gttccagcat atcttctgtc aacacgcgtt tgatttgttc
     7981 gattggcatg ttgtatgttt ctgataattc ttggatttct gcattgattt cttcttctgt
     8041 tgcttgaaga ttttcagcag ctgcaattgc ttcgatcaca agatttgttc ttgtgcgcat
     8101 ttctgcttca ccttcgaatt gtttatgcaa gtcttcttct gtagaaccag ttaattgata
     8161 gtacatttca ggagagatac cttgacgttg catgttgttc aagaattcgt ccattgaacg
     8221 atgtacttca tcatgaacca tcacgtgtgg caattcaacg atttctgcgt tttcaacagc
     8281 catacggatc gctgcttcat cttttgcatc gtccgctgct ttttcttttg tttctactaa
     8341 ttctttgcga tatttttctt tcaattcgtc taatgttgat acttcgtcat caacatcttt
     8401 tgcgaattcg tcatctaatt caggcacttc ttttgctttt acttcatgga ttgtcacttt
     8461 gaagactgct tcttttccag caagatcttc tgcttgataa tcttctggga aagttacatt
     8521 tacatctaga ctttcacctg atttatgacc aactaattga tcttcaaaac ctggaatgaa
     8581 tgagcctgaa cctaattcaa gtgagtaatt ttcgcctttt ccgccatcaa acgcttcgcc
     8641 atctacaaag ccttcgaagt cgattactac tgtatcgcca ttttcagctg cagcatcttc
     8701 tttgataact aattcagctt gtgcttcttg ttcacgtttg atacgtgcat ccacgtcagc
     8761 atctgttact tcacggtctt gtttttcaac aactagatct ttatattgtc ctaattttac
     8821 ttctggtttc actgttactt cagcagtgat tacccagtct tgaccttttt ccatgctttc
     8881 aacatcgatt tttggttgag aaacaggatc gatgccagct tctttgacag cagcttcata
     8941 agcttctggt aatacagcat tcaatgcatc ttcatataaa gcttcttcac cgtacatacg
     9001 gttgaatact tgacgagaaa ctttcccttt acggaatcca ggtacgttca agcttttttt
     9061 cactttatta aacgcagttg ttaatccttt ttggatttcg tcttgcgcaa tcgtaaattt
     9121 taaaacacca tcattcgtgc cttttttttc aaatgttgca gtcatttaat tccctccgag
     9181 tttagaaatt cattttatac atcaaaaacg tttcatgcat acctttaaat agtacactaa
     9241 ccctttcaaa ttgtaaacat attgaatgga tagaatcctt gtttttcata acatttttat
     9301 ctttttcact gataaaatct aatggaagaa gatcatttgc tgcaaaattt tttctctttt
     9361 ttttaaaaaa tcttgttttt catcttctgt accttcatac atcgtttttc ctgtacacgc
     9421 ctctacagtc agttgaaacc agcgcttcgg gtcttcgatg tatgtatcat agacaggata
     9481 aacaattgca gattgcaacg tgaattcttc ttttaacacg gaactaagca gcgcatcttc
     9541 attcccgtaa cgatctgaaa atacacataa ttgctggtaa cttgcctgct gctgcggctt
     9601 tggcagatct tttggtatga ctttgactaa cttttcttca atcgtcaaat agaaaacagg
     9661 atggcattcg ttcaattgcc taagtgtctc cagtaatttt gcccttgcta aaatatggac
     9721 ctctggcgat acaataagtt tactcgctgt ttggataaac tcatgataag gaagctgttc
     9781 gatctcatca atagacgcaa gctgacgtgc aggttccaac gaaggaagtt cctccgattt
     9841 attgtggagt gccttgatct tctgcctctc catttgtccg gataattctt ctgcaaattg
     9901 gattttttca atgacccgct gctcgaaagc ttcataaaaa tctgtacttg ctatcaattt
     9961 tctagcatat aaaaaatctt gtgtactgat taacaagtca aaataaaatt cagcaaactc
    10021 ctcattttga agatactgtt tttcatgcaa gagtgcttgc ttcaatgctt cttttttctc
    10081 gtccaactcg taaagacaaa aaacaagcag gctattagcc tcaaagcttt ctttcaactc
    10141 gtaagctcgt tgaaataatt cttttgcctg ctgatattgc tggtttgcta gtgcttgttg
    10201 tgccactcgc attaaattct catatttttc tggaaaatca atcggcttca cgcagtgtcc
    10261 tcctcttgct catattcagc tcatgttcaa tagtagcaaa acaaaaaaag catgacaatt
    10321 aaaaataact gaagcaccct ctaaaagcta gattttagat ctgactttta gaaggtgctt
    10381 tcaactgtta ctgcttttta ttgctcatca gctgttgact tcctggttga acgctttgat
    10441 cagattgatc gtaacaacac ttgtattagt gaaaaaatcg cccagatgtt gctttttctg
    10501 attaaagact gctggaagat accctaacat cagcaaagga aattttagaa tgaaccggca
    10561 agcaccttct ctaactaaaa cggttgacca gctaagttct tcttcatcaa agctgatgac
    10621 tcgaatacca aagatcattt tcccaatcgt ttggccatga ttgaatttcg tcaataaaat
    10681 aaaatacgct agataaataa gtaaggctgt gaatccgtag agactcagat aagaatctga
    10741 aaaattaagc caacctagat ttttggcaat accaagtgtt aaacgcgtaa tagctccgat
    10801 acacaaaaga tcgaccaaga aggcaaacat tcggataaaa aaacctgcat agaaatactt
    10861 aggaaaatca tttgcttttg gcggttcatt tcttgtgtcc gtatactttt tccatttacg
    10921 ttgtttcttt ttgatttctt cgggcgtcaa aggctctctt tcaaaagagc tcagtacctt
    10981 ttgggaaaag ttggagtcag gtcctacagc aggctgctgt tgcttatctt tttcttgttc
    11041 cttattttca tttgaaagct ctgacatgct tactcacctc cgtaataata cattgctttt
    11101 ggtgattccg ctgtccctag attctcaaga acggataaga tctgactagt ctcagaaggc
    11161 ttcaatccct ggaattctgc tagtttactt cctaaccatg tactagcaaa cccagatggc
    11221 gtggaagaat attctactag ttccgcatct tcaagctgtt gttcctttcg tatagctttt
    11281 aaagcatctt caggaaatcc caattcatcg actaaaccta cttcttttgc ttggacacca
    11341 tcataaatcc ttccatcagc aatttttttg acttcttctt cagatttatt ccgtccctcg
    11401 ctgacgattg ctacaaaacg actatatgcg ctgtcgatat atgcttgaag caccgcttct
    11461 tcttcttttg tctctggtcg cgttcctgaa cccatatctt tcaacgctcc gcttttaact
    11521 gttgtatctt ccacacccag tttttctaga agtcctgaat aattcaatcc tgacatgatc
    11581 acaccaattg agccggtcac tgtttcttct gttgcaaaga ttttatctgc ttgagcagaa
    11641 atatagtagc caccgctagc tgccatgttt ttcatgctga catacattgg gatatcatgt
    11701 tctttacgaa tcgtatccaa tttcttagcg atttccgcac tttcgtagac accgccacca
    11761 ggagaattta cttctaaaaa gatacctttg actgttggat cttcttcaat cgccttcagt
    11821 tgttccataa aatttttatg attgtaacct tcagaagcaa ataaacctga agaccctccc
    11881 gcagcaatcg ttccatctac tgataatttt acgatttttt gcgtactgtc tccctcttcc
    11941 aacacatttg gagctagttc ttcatcccca tataaccaac tgttcaatcc ctcaatttga
    12001 tcgttttcct gaggcttagt caaactagct gaaactaacg aaaccacaaa taatcctgca
    12061 gcaatcagaa ctgctgccca acgtctttta ttcattcttt ctcctttcta atacaagttt
    12121 tttatgacga tcacagtttc tgactgctca gaagtgtcga accgctctgc tttttcttcc
    12181 cgcgcatacc cggaaaacga ctcttttgtt aattcatctt ctaccgtaaa ctcacgagct
    12241 tgagcaactt ccaaggcatc aggtgcgatt tcttggatgc tatcccagcc agtatctaaa
    12301 aatattttcg tatcatctga tacaccagct gatgcttcga atttggacaa ttgttttttt
    12361 aacattccca gtgtcatgat ctcaattttt gaatccattt ttactcctcc tctatccgat
    12421 tactttctat tgtaccgaac tcctgaaaaa gatgatagat aaaaacccat tattttttga
    12481 cataaaaagg acatttgaca aagaatttga tatataccaa tttatttttt attctttttt
    12541 tgttgacaag aacattcgca aaaggttatg attttaaatg tttccagata aaaaacaaaa
    12601 aggaggatag ttataccttt taggatagcg ccaggtctgt gtaaacagat gacgaggaga
    12661 gggtttatcg aaagattcgg cggatggccc tagggactgc actctacaga aaatccacaa
    12721 aaaatgtctg caaagataaa acaaaaggat tttcctgcag ctggaagcat tcatacgaat
    12781 tcattataga aagtaggatg ttttttatgt gcggaattgt tggaatgatt ggattaaaaa
    12841 atgtaacacc aggattgatt gacggtctag aaaaattaga ataccgtggt tatgattcag
    12901 ctggtatctt tgtctcagat ggcacaactg attatctggt caaagcacaa ggcagaattc
    12961 aaaatttgaa aaacaaaata aatattgata ctacaggaaa tataggtatt ggtcatacac
    13021 gatgggcaac tcacgggcaa ccttctgaag aaaacgccca tccacatact tcccaaagtg
    13081 gacgcttcgt gctcgttcac aacggcgtaa tcgaaaactt tgaagaactt aaaattgctt
    13141 atttagcaaa tgaccacttt attggtgaga ccgatactga gattatcgct catctaattg
    13201 aaactttcgc gaaggatact acaacgcaag aggcgttttt aaaagctttg cgggtcatca
    13261 aaggttctta cgctttcgct ttgattgatc ggacagcacc tgatgttatt tacgtcgcta
    13321 aaaataaaag tccactatta atcggactcg gcgatggatt caatgtgatt gccagcgatg
    13381 caatggctat gcttgctcat acgaaggaat tcgttgagat tgaagatgaa gagatggtta
    13441 ccgtcacatc agaaaaagtt attattcaaa attttgcagg tgacatcgta gaacgttctt
    13501 cattcgaagc acaagtagat gcttcagata tcgagaaagg tacctatcct ttttatatgc
    13561 tgaaagaaat cgacgaacag cctattatca tgcgtcgtat cgctcaaaaa tacgtaacag
    13621 aagacaatcg tgtctctctt gatcaaaagc tggtcgatac attgtctgat agcgatcgaa
    13681 tctatatcgt tgcttgcgga actagttacc atgcggggtt agcaggtaaa cagactttgg
    13741 aaaaattgac acagattcct gttgaggttc atttagccag tgaatttggc tataacacac
    13801 ctcttctttc aaaaaaacca ttctttattt tcttgagtca aagtggcgaa acagcagaca
    13861 gccgccaagt gctggtaaaa atcaaccgtc tcggctaccc ttcacttacg attacaaatg
    13921 ttgctggttc tactctttcg cgggaagcat cctttacact gctattgcat gctggacccg
    13981 aaatcgcagt agcatcaacg aaagcttata ctgcacaaat agctgtacta actttattag
    14041 ccaaagctat cggtgataaa aaagaatttg aaacttctct tgtattcgat atcactcatg
    14101 aattgagttt agttgctaat gcgatggaat cggtcatcgc ccaaaaagat tatttagaag
    14161 agttggcaga agtatatctc agcgatacaa gaaatgcctt ttacattgga cgtggtgctg
    14221 actacaacgt ttctcttgaa gctgctttga aattgaaaga aatcagttat atccaggcag
    14281 aaggttttgc cgctggcgaa ttgaaacatg gaacgattgc tttgatcgaa gaaggaacac
    14341 cagtgatcgg aatcatcagt gaagaagtga ctggtgcgca tactcgagga aacctgaaag
    14401 aagtcgaaag ccgtggcgct aaaacactag ttattgtctc agagggatta gaaaaggaaa
    14461 gtgaccagct ggtgctacca actgtccacc cttatctaac tactctaacc acagtcgtac
    14521 cgactcaatt acttgcatat tacgcaacgc tattacgggg attcgacgtt gacaaaccac
    14581 gtaatttagc gaaatcagtg acagtagaat aataaaagaa caaggaccag tgtcagacat
    14641 ctgacactgg tccttgttct tttatttttt atgaaaatat ctcttaatcc agtccgattt
    14701 cttctttgac tactgccgcg attttatcga catagtaatt aactttctca tcagtaggcg
    14761 cttctgccat cactcgaagt aatggttctg taccggaagg acgaactagg attcttcctt
    14821 ctgatcccat ttcagcttct gcctcttcaa tcactttttt gattgccgga acatccatcg
    14881 caccgttttt atcacttacg cggatattca ctaatttttg cggatagatt gttacctctg
    14941 ctgcaagctc tgataatttc ttacctgtct gtttcatgat attcagtaac tgaattcctg
    15001 aaagcatccc atctccagtt gtgttaaaat ctaagaaaat catgtgccca gactgctcgc
    15061 cgccaaagtt ataatcgttt ttgcgcattt cttctacaac gtaacgatca ccaacttgtg
    15121 taacgacatc tttcaaaccg attccttcca ccgctttgtg gaatcctaag ttactcatga
    15181 cagtcgtaac gatcgtatct tttttcaatc gatttttttc tgccaagtat ttagcacaga
    15241 taaacatgat tttatcaccg tcgacaatgt ttccaagttc atccaccgca ataatacgat
    15301 cgccatcgcc atcgaaagct agtccagcat ctgctccttt ttcaacgacc atctcagcta
    15361 aacgttctgg atgggtagag cctacgccgt cattgatatt taacccgtct ggatttgtgc
    15421 ccattgtata aaaatctgtt tcaagatcgg caaacaaacg attaacactt gttgaagttg
    15481 ctccgtttgc tgcatcgaca caaatggtca aaccggaaag gtcaccgttg atcgtttgta
    15541 caaggaattg agaatatttc aataagcctt ctggaaattc ttctacagtt cccaatcctt
    15601 cagctgatgg acgaggcaat tcatccactt cagcatctaa taatgcttca atttctagct
    15661 cttggtcatc tactagttta aaaccatctg caccgaaaaa tttgattcca ttatcttgtg
    15721 ctggattatg agaagcagaa atcatgaccc ctgcgcttgc tttttgcaaa cgtgtcagat
    15781 aagcaacacc tggtgtagag atcacaccta gctggaacac ttcgataccc accgataaaa
    15841 gtcctgaaat cagcgcttct tctaacattt gacctgagat tcttgtatcc cgccctacta
    15901 acacgcgtgg gcgttttcct tcttcttcat gctggcttag gacataacct ccgcaccgtc
    15961 ccagcttaaa tgcaagttct ggcgtaagtt ccttgttggc ttcaccgcgt acgccgtctg
    16021 ttccaaaata tttacccata gttgtaaaat tactccttta acttaatttt ctgaggaatt
    16081 tgttctgctc atttgtgttg attcgcttgt ttctgatgtt tcttctgtac ttgtggtcga
    16141 gctgcttgtc ccagattccg aactggaaga aatggttgtt gtcgatggtt ccgttcctga
    16201 atcagcagat gaaatacttc tactcgtact tgtattttct tgtgtccgac tgctcgtcga
    16261 agcagagcta ctgcttgacg tttgcaacac gggtgtaatc tccacactaa ctgattcggg
    16321 cttgatactt aacccctctt ctactggtat ttcaatttta cgagttgttc gatgattgat
    16381 acctgagaca ttgatcggta cagcaatact gtcaccgatc gatgcaagca aatcgctaga
    16441 gccgcttact tctgcttcca gttttgaaaa actgaaatca tagctttcta ccccggcagc
    16501 tggtgttcct tgctgtatcc cataaagtcg taccgacttg cttggcttga tcgaatcagc
    16561 tctcactgtt acttgcactg gatcagagac gatacttaat acttcccctg ctgcattcag
    16621 cgcctgtatt gggacctcac tattaatccc atcatctgtg accatcgaag cgtccacatc
    16681 agctactact cgtttgattt ctgccaatgt tttgtcacct gtagtgatag agactttttt
    16741 aggactaacg gaagtatcat caagttcata tccagatggt gtgaacgttg aaggaatcaa
    16801 cgtttccaca tcgaaatctt ttattacctt tttctcaacc gttactgtaa tagtcgatgg
    16861 ttcgatcttt gctgtgacag cactgctcaa attttgaatt tttagcggaa cttcatgtgt
    16921 tccttcgccc agcttagtga tatctgctgt gacgcggaac atccgtgtat cttcgtttgc
    16981 ctctgcattc aactgaactc ggtttgcact ggaaagtttg acagtcactg cattttcata
    17041 tccatggata tagtagcgct ttgaatcata agaaatcttg atcggcaccc ccgtaactgt
    17101 ttgattatac gcttcatttc cagaaagcgt atttggaacg gactggccat ttgcattgaa
    17161 gtacagcgca atactgaaca gcaatgcaat aaaactgtaa agaatgcttg tccgtttctc
    17221 ttttgagatc atttgcgatc ccctccctta gtcataccgt ctaggaaaga ttgaatcacg
    17281 tttttcttgt tttttgtttt ctcttcagca attagttctc tacgcaagat tgccaaatat
    17341 tcttgctggg ataagccagc catcagttca ttatctaacg tgatgctgac gtccccggtt
    17401 tcttcagaga caacgatggt aatcgcgtca ctgacttcgc ttataccgac agctgcccgg
    17461 tgtcgagtcc cgaattcttt agggatcaac atactttctg atagaggaag ataggcgctt
    17521 gctactgcga tctttccttc ttttacaata actgcaccgt catgcagcgg tgtattagga
    17581 ataaagatat tgatcagcag ctccccagtg atatctgcat ctaaagcaat ccctgtctca
    17641 atatactcat ctaatccagt atgacgttcg atagtgatca acgcaccgat ttttcgcttg
    17701 gacatatatt ggatggcttt gtcaaaagac aagatcatct tctcatcttc ctgctgttcg
    17761 ctttttgatt gtttgaaaag cgaactgcgt ccaagatgtt ccagtcctct ccggatttcc
    17821 ggctgaaaaa tcacgatagc agctatcacc ccgtacgtaa tgacttggtt catcagccaa
    17881 gacaaggtgt gcagaccaat gatttccgct acgattcgga taacaataaa aatagctaca
    17941 cctttcagaa gctgaatcgc ttttgtgcct cgcagcagtt ggatcagttt gtatacaaga
    18001 taccagacaa ccagaatatc aagtatgttc accaaaaaat tcagagagaa caagtcaccg
    18061 gaaaacattt gctgccaata gtccaaatta aaaagttccc cgatttgaaa tgacataaaa
    18121 agcctctctt ctcttttgag atttcctctc tagtatatca tatgttttaa ttttagaggc
    18181 tgatatgctc gtatttttct tgagaaaaac gcataatttt caagaaaatt gcaagtatgc
    18241 gttttatttt tctctgcaat aaatattttt aaacttcaga gaattattca ttatattttt
    18301 tctgttattt ttcctttttt tcttttgtag cttgttccat ttttttacgt cgttctgctc
    18361 tggcagctct tctagcagct cttctttctt ctgcttcaag gtccgtcgtt ttttcttttc
    18421 tagtcttaac gggtacatcg atagattcct ctggttcaag tcgtttcttg attttttcta
    18481 attctcccgt cagtgcagca tagttgtaaa aacgtgtttt agcgtcgtgg acagtctttt
    18541 caaataaagc ttttgcaaga tttggatttg cgttatctag cgaggaaaag cgaacttgtt
    18601 tcctcatgaa atcaggcata gcagagaaat caggtttttt gtagtccaaa gtcattgggt
    18661 tcttgttttt ttcgaccatt gtagggttat atcgatataa tgaccaataa ccagaagaaa
    18721 cagcttcttt tgcttcttta agcgattctt tcattccgcc aagcaagcca tgtgtgatac
    18781 aaggcgtata ggcaataatc agagaaggac caggatactt ttctgcttct tcaaatgcct
    18841 tgatcgtttg catttgattg gcacctgaag caatttgcgc cacataaaca tttccatatg
    18901 tcatcgccat cattcctaga tctttctttg aagcatactt tccactagcc gaaaatttag
    18961 cgattgccga agctggtgtt gcttttgagg tttgaccgcc agtattggaa tagacttcgt
    19021 tatccaaaac aaggatattc acgtctgctc cgctggcaag tacatgatcg atcccgccaa
    19081 aaccaatgtc gtaagcccat ccgtctccac caatcatcca ttggctgttt tttacgaata
    19141 gatcctttct cttgtatata tcctccagtt cagggaaaat tgatcgttcg ctttcgatcg
    19201 cagcttgcag ttttgctgct ctttgctgcg ttccttcact ctcaaacatg tgtactaacc
    19261 agtcttccat caattctcgt agttcttctg atacttttgg caatacttct ttgattgaac
    19321 ttgccaaagc ctcgcgacga gtctgtgccg ctagaagcat accataacca aattcagcat
    19381 tatcttccaa taaagaattt gaccatgcag gcccttgacc acattcattg gtcgtataag
    19441 gagaagcagg agaagcaccg ccccaaatag aagaacagcc tgtcgcattc gcgatcatca
    19501 ttcgatcacc gaacatttgt gtcaataatt ttacataagg tgtttcgcca cagcctgagc
    19561 aagcgcccga gaattcaaga agcggttgat tgaattgtgt agacaagaca gtattctttc
    19621 ctttgactgg atttgctttt tgtcttaatg tcattgcaaa agcccaattc acagcttgtt
    19681 ctttctgttc ttcataaggc ttcatcacta aagctttttc ttttgcggga caagcctcta
    19741 cacacagacc acaacctgta cagtcttcca ctgaaacttg gatacggtat tttacgccgt
    19801 ctgcgcctcg catttctctc acgataaatc cttctggcgc ttcttccatt tcctcttcat
    19861 ctgctaaaaa tggacggatg gcagcatgag gacaaacaaa ggcacattca ttacacatcg
    19921 tacagcgatc actaatccac tcaggtactt ctaaagcgac gccccgcttt tccatagaag
    19981 ccgttcctaa aggcatttcg ccagttgtca ttccatttgc tacaagtgta ccaacagaga
    20041 gttgatttcc ttcctgtgca ttcactggtt caagtatttc ttgtatataa ccagtgttcc
    20101 ttttattgat tttttcaggc atctccaacg ttctccaatg gtctgggatc gcgacttgat
    20161 gaagcagttc ggtcgtccga tcgattgctt gtatgttttt ttctacgata gccattgatt
    20221 tatgtccata gcttttcagg gcttcttctt ttagcagcgg aagcacctca tcaaaaggaa
    20281 tgatgtcagc taatttaaaa aaagccgttt ccattgccgt attgatccgg cgtcccaaac
    20341 cgacttcttg ggccaatctc atggcattga tcgtatagaa tctaatgtta ttttcagcca
    20401 agtaacgttt taattttaag ggcagatgcg tttctaattg ttcgtcgctc caaagagtat
    20461 tcaataaaaa aatgccacct ggcttcaatc ctttgaccaa gtcatagcta tgcaggtaag
    20521 ctggtgtgtg acaggcaaca aggtccgcat gctcgacaag atacgctgaa cgtatcggcg
    20581 tatctccaaa acgaagatga gagacagtca gaccgccaga ttttttcgaa tcataataaa
    20641 aatagccttg cgcatattta tcggtgtgat ctccaataat cttgatagct gatttatttg
    20701 ctccaactgt tccgtcagat ccaaatcccc aaaacttggc ctggaatgtt tgcggttctg
    20761 tcaaatccag actttccttt ggttccaaag attgataagt cacatcatcc acgatcccta
    20821 tggtaaaccg ttttttgcgt atgcttgggt cttttttcaa ttcatcaaaa actgctgaga
    20881 tttggtcggg agtcacatct ttagacccca atccgtagcg accaccaata acagccgggc
    20941 gaagttcaga atcatataac gcactttgaa cgtctaataa gagaggttct ccacctgccc
    21001 ctggttcttt acttcgatca agaacagcaa ttgtttttac tgtatgaggc aacttttgca
    21061 cgaatgtttc cactggaaaa ggacgataaa gatggatatt caaaaaacct gttttccgtc
    21121 cgtttgcatt caagtaatca atcgtttgtt caatcgtctg tgcaactgat cccatagaaa
    21181 caatgacttc ttccgcatcg tctgctccgt aataagtcac caagtcataa gaagttcctc
    21241 gtaatcggtt gatctctttc atatattttt gaacgatttc aggtaattta tcataatagg
    21301 ggttgatcgt ttcccgttgt tgaaaatgga tgtctggatt ctggttcgta ccactaacag
    21361 tcggatgatt gggattcatc cctcgtttac gaaatgaagc taatttctcc tgattgatca
    21421 gaggagctaa ttcttcatat tccaaaactt cgattttttg aatctcgtga ctcgtacgga
    21481 atccatcaaa gaaattcaaa aaagggactt ctccttcaat agaagcaaga tgggcaacag
    21541 gtgctaaatc catcacttct tgtacactgc tttcagctaa catagcaaat cctgtttgtc
    21601 ttgcagccat cacgtctccg tgatctccga aaatattcaa cgcatttgtc gtcactgccc
    21661 gactagcaac gtggaaaaca cttggcagta gttccccagc aatcttgtac atattcggta
    21721 tcatcaaaag cagcccttgt gaagcggtat atgtagttgt cagtgcacca gttttcaatg
    21781 atccatggac cacaccagct gctcctgctt cggattgcat ttctactact tttactggtt
    21841 gaccgaatac gttttctttt cctgctgctg accattgatc tactaattca gccatggtcg
    21901 aactgggtgt tattggatat attgcagcaa tctcggtaaa tgcataagaa atatatgcgg
    21961 ctgccgtatt gccatccatt gttttcattt ttcgcatgat tcatcatcct tgttcatatt
    22021 ctctcttatt atctgttcca ttataacgta aaacatgcca tttttttctt ttatttataa
    22081 aaatatttta catgtatcta tttgaattgg tcatctaatt tttagacaaa cagtctaaat
    22141 cgttgccttt ttttgtcaga catgtataaa ctataagcga aagaggtgtt ttaacatgga
    22201 tatatcagtg attgatgcaa cgaaaacaaa tacacaaaaa ggaatccttt atggttcatc
    22261 gaatgctcct aaaaaaatgg tcgaattcat caacttagct tgtccgtatt gccgacaatg
    22321 gtttgaagaa tcttatgagc tattagaaga agcagtccaa agcgggcaat tgcagcgagt
    22381 gatcaaattg ttcgataaag aaaaagaaag cttacagcga gggaacgtga tgcaccggta
    22441 tctcacaatc agcgatggcc aaaaagcgat caaagagatc aaacaaatat tcgatactca
    22501 agacgagtgg aaacatcttt ctctccaaga agtagcggat tttgcagtag ataaactaaa
    22561 gctgacagaa caaaaagacg aacagctgag ccaagctgtc atcaacgaag ctgagcaagc
    22621 acatattcga tttgttccaa ctgtcatctt gggtaaagaa atctttgatg aatctatttc
    22681 aatagaggaa ttaaaagaat tgatccaaac aaaataaaaa aataaggagc gtgggacaaa
    22741 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac
    22801 tccttcttat ttcttggagt taaacgcttc tgtcccagcc tccttattcc gaataaatgg
    22861 tgtccaaaag cttgcatctg ccagattatc tcatttctta tgacaagcta agcaaattat
    22921 ctccaccaaa taatttattc atatttttga cttgcgcata ctaactaatt cttaaatttt
    22981 catacaaaat ttctcaattt cccttaacga gtaatattta tgtacggtga ttctcactag
    23041 tatcccaatc actttttaag ttatcccgat ttcaattcta cttcaaaaat agttgctttt
    23101 atgtatgctg ttagttgatt agatggtcat ttgttgagct aggtcgttta ttttaaacaa
    23161 gttgtttctt tagcttatac ctatctcgtc taccaaacgc aagatggcaa acacaaatgc
    23221 atacgcatct gtatttgcca ctttgattcg tataaagcta acaactatta actcctctca
    23281 gctaatagtt gctctctttt ttattcagat tcatttttgc tgatcacagc ctagtatttc
    23341 aatggttttg aacactaaac caaattgttc cattacaaca ttcactggct gatgaaatgg
    23401 attagtagat ttcgattatt caaagcgatt ttctattggt ggatcgtgtt cccaactaat
    23461 aagacggatt ccaaacctat cgtacagatc ttctaaacca cttataaatt ttgcacctat
    23521 ttcaggtgtt ttattgcgtg gatattctat tgttacgcca ttaaatggat accaattagg
    23581 attaggctct ctttttttct gtacatctcc catagaatag ttagcacttt ctgggacata
    23641 atatattctt cgaccatcct ctaattcatc taataagata tttcgtgatc gtctatcact
    23701 tgctaaatgt agacgccacg tgcgatacca tgccatgcta cctgcatctg cagaaaatcc
    23761 atcaaataat gcaacaattg tatcttctat cacaatcctt tccccatcta cttcatattc
    23821 taatctaaaa tcaaactccc ctcgggtaat ttctggtctt ggtggcttag gacccaatat
    23881 tattctactt aaaaccgcat tcgttcccag atgaaacaca gctataacaa caataatgat
    23941 taatattttg tttctatttg ataaatttct cattattcgc acatcacctc tacattctca
    24001 catattagtt cgtaatactc ctttaatatc aaaatatcta ctggtcgtaa taatattcgc
    24061 tatgaattcc tactactcgt ataagaaagg ccaaatcatc agcttttact tgctgatgat
    24121 ttggcttgtt tctatttatc gtaattgttt tctaatcacc aaaattgtta acaatcggtt
    24181 cagatatttc ccagttgatt aaggtaattc cgtattgtgt caaaacttct tgcgcttctc
    24241 cgaaattaag tacagttctt atgtatccac tttctcctaa tataaagtct acctctcttt
    24301 taaaaccttc ctcgagattt ctaagggcta tatgtggttt taatgacatt gattgtacat
    24361 tttcccccat ataatattgc gcacttcctc gactaaaata gattcctaga tcgtccccaa
    24421 aaggtagatc ttctctacca ctagctaatc tacttctcca tcttcgttct cttttcatag
    24481 aacctgcgct gaactcacac ccgttaaact cagctatgac agtatcttca attatgttaa
    24541 tctgtccatc tagttcgtat tcaatcctaa atggaaattc tccataggtg atttccggtt
    24601 cgggtatttt aggtgagatt aatgcaaaga aaggaaatat taagcttggc aacatcaaaa
    24661 aaactcctcc tatgatcaca attcccgatg taatccaaaa tataatttgt acaattctca
    24721 ttgatccatt gtattccatt ttattccccc tattactatt catatccgcc tatctatatc
    24781 gatcttccaa tgcagagata attgcttcca taccttgatt ttttactgag ttacctatgc
    24841 gagatatcat tctccactaa ggtgcatcat attgtctatc taacatgatt aattctccaa
    24901 taggtgtata ctcctccaaa aacaacattt aatgcatctc cctcgacaac ataagctacc
    24961 atgtttgctg tatatgtgtt tgagtctaat tcataagcag ataaatttac tgtcgctgga
    25021 ttaaatataa tcgcattcgt atttatagct actgaatttg ctgcttcagc accacctttt
    25081 gaatgtccga tcattgtgat ttcaatagta ggatttacgt ggacagccct caccgtttac
    25141 cctgaccatt atagaaatcg cttacttttt tgaactacgc aacaaaggat acaatgctcc
    25201 taacttcact gtctccattt ttgtccacta aggaatataa aaaataggca ttaattcatt
    25261 taattcggtc acaacacgtt accttttaaa tcattatact gtatctcata cattattatc
    25321 aataaactat tataaaaatg caaaaaatgc tttatcaatc tattttgtaa ttggtcataa
    25381 tatcattgaa atcataccct gtatcgattt ttcctatgaa tctcgtctat ttgtgattga
    25441 cgaaaaagag gcaagacaga cactggaatg gtaattttac ccactatttt gctccaacta
    25501 tttgtaatcg tacccacttt tgaaataccg agatgatcat tttaattgat atgctgattc
    25561 ccaagcaaaa ccccaagaaa ttaaatgctc tatcgcatct agcttcttgg gttttgctta
    25621 tataaattcc aacatttaaa ttttttatgc tgttcggtct cagaaatgcc gtttttgctt
    25681 atatcacaat atcaagatgt caaattattc gtgttgccga tgactttgat taattttttt
    25741 caaattgttt attaagacta atgctgtctt tttgcagata ccaatgttcg aactttttaa
    25801 cattatcata tgatcctgca agttttaact tcttaccatt agatgttatc aaagttataa
    25861 ttaaccttgc ctttttccca ttaggatctt ttattttatg aattttctgc aggtaatctc
    25921 tcctaatggc agctattttt acctcatcag tgctgataaa ccattgatca tccacataca
    25981 tactgcctgt tatgttgtgt ttttccattt cttccgagaa cgaaaagttt aaggctcgtt
    26041 cttacttcca aagttgaatg agtccatgga ttggacttag tgtgagaatt gagaacaaca
    26101 ataagaatcc atatcctccc gcaaacatcc ctaaggctat actgtctctc catccataag
    26161 taggattaga agagaaaaga gtcaccgttt gtaataatat gaaaaggaat aatccagcac
    26221 aaacaccaaa gataacaact ccaaacattg aatttttata caatgaccat ttatttatag
    26281 gcttttcttt cattttactt ccccctactg ttctgttcac acggaatgaa ttcaaagttc
    26341 ataagccctc ctctttgaac atatgaatac gacttgctaa ctatacttta aaaaagcttg
    26401 ttttaagcgg ttataggcga gttgtaatct ctattcatct cattagcaaa taacttgccg
    26461 aaaagtctac tgaaattaaa gcagatagtg gaagaattat ttagccagga tagacactat
    26521 ttgtgaaaca catgtcttta cttttgaaat tctatgatga aaagccttgg aatacagtag
    26581 ctcttaattg gtaatacttt ttccattata aataacctct tttcatttta tatttatccc
    26641 ctctaaaaat aatatctaat ctaatatttt aaacttatat ttccctcgct gtgctaatag
    26701 agcattacta aataaataga aactagaaaa aagcattacg cctgccagaa taaaccaatt
    26761 atttgt
//
LOCUS       DACCXM010000027        26751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCXM010000027 DACCXM010000000
VERSION     DACCXM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.029"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            37..258
                     /locus_tag="IVY61_000960"
     CDS             37..258
                     /locus_tag="IVY61_000960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970089.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            385..1161
                     /locus_tag="IVY61_000961"
     CDS             385..1161
                     /locus_tag="IVY61_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7970090.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            1158..1352
                     /locus_tag="IVY61_000962"
     CDS             1158..1352
                     /locus_tag="IVY61_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7970091.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            1354..1638
                     /locus_tag="IVY61_000963"
     CDS             1354..1638
                     /locus_tag="IVY61_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970092.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            1659..1847
                     /locus_tag="IVY61_000964"
     CDS             1659..1847
                     /locus_tag="IVY61_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970093.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2173..2412
                     /locus_tag="IVY61_000965"
     CDS             2173..2412
                     /locus_tag="IVY61_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970094.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            2418..3104
                     /locus_tag="IVY61_000966"
     CDS             2418..3104
                     /locus_tag="IVY61_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970095.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            3107..3937
                     /locus_tag="IVY61_000967"
     CDS             3107..3937
                     /locus_tag="IVY61_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7970096.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            3954..4805
                     /locus_tag="IVY61_000968"
     CDS             3954..4805
                     /locus_tag="IVY61_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7970097.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            4802..5161
                     /locus_tag="IVY61_000969"
     CDS             4802..5161
                     /locus_tag="IVY61_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP7970098.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            5176..5337
                     /locus_tag="IVY61_000970"
     CDS             5176..5337
                     /locus_tag="IVY61_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7970099.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            5340..5645
                     /locus_tag="IVY61_000971"
     CDS             5340..5645
                     /locus_tag="IVY61_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970100.1"
                     /translation="MDKKATMKRIVELTKSESWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKKC"
     gene            5639..5929
                     /locus_tag="IVY61_000972"
     CDS             5639..5929
                     /locus_tag="IVY61_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970101.1"
                     /translation="MLDMRIEDYRITSDSRNIVLSKVRRDEEGNIRYTEAKEESRADI
                     GYFQTVSSCLKAIQRDYVLSEERTIKSIIEYKKALENITRQFEQACEIEEEK"
     gene            5929..6246
                     /locus_tag="IVY61_000973"
     CDS             5929..6246
                     /locus_tag="IVY61_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970102.1"
                     /translation="MEFVDLNVRGIKCDNPECDYSDMAVKYENYPQWLNKPCPKCGAN
                     LLTQEDLDATEQLMEIVNLTNEILKDSGLEKQDMNKYIVPVEANGTGELSFGEIKKLE
                     EEK"
     gene            6243..7058
                     /locus_tag="IVY61_000974"
     CDS             6243..7058
                     /locus_tag="IVY61_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP7970103.1"
                     /translation="MNENKLIKLGVAGVVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKEHGWVTVNGADTVVTDETGK"
     gene            7197..7511
                     /locus_tag="IVY61_000975"
     CDS             7197..7511
                     /locus_tag="IVY61_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7970104.1"
                     /translation="MDLITQYSEIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            7688..7999
                     /locus_tag="IVY61_000976"
     CDS             7688..7999
                     /locus_tag="IVY61_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970105.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEEA
                     E"
     gene            7996..8199
                     /locus_tag="IVY61_000977"
     CDS             7996..8199
                     /locus_tag="IVY61_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP7970106.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            8196..8492
                     /locus_tag="IVY61_000978"
     CDS             8196..8492
                     /locus_tag="IVY61_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970107.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            8569..8985
                     /locus_tag="IVY61_000979"
     CDS             8569..8985
                     /locus_tag="IVY61_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP7970108.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            9891..10235
                     /locus_tag="IVY61_000980"
     CDS             9891..10235
                     /locus_tag="IVY61_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7970109.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            10240..10521
                     /locus_tag="IVY61_000981"
     CDS             10240..10521
                     /locus_tag="IVY61_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970110.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            10625..10939
                     /locus_tag="IVY61_000982"
     CDS             10625..10939
                     /locus_tag="IVY61_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP7970111.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10917..12611
                     /locus_tag="IVY61_000983"
     CDS             10917..12611
                     /locus_tag="IVY61_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7970112.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            12631..13809
                     /locus_tag="IVY61_000984"
     CDS             12631..13809
                     /locus_tag="IVY61_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7970113.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            13772..14458
                     /locus_tag="IVY61_000985"
     CDS             13772..14458
                     /locus_tag="IVY61_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP7970114.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            14458..15618
                     /locus_tag="IVY61_000986"
     CDS             14458..15618
                     /locus_tag="IVY61_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7970115.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKEAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            15628..16503
                     /locus_tag="IVY61_000987"
     CDS             15628..16503
                     /locus_tag="IVY61_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970116.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            16500..16811
                     /locus_tag="IVY61_000988"
     CDS             16500..16811
                     /locus_tag="IVY61_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970117.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            16801..17154
                     /locus_tag="IVY61_000989"
     CDS             16801..17154
                     /locus_tag="IVY61_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7970118.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            17144..17545
                     /locus_tag="IVY61_000990"
     CDS             17144..17545
                     /locus_tag="IVY61_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970119.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            17538..17942
                     /locus_tag="IVY61_000991"
     CDS             17538..17942
                     /locus_tag="IVY61_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970120.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            17954..18559
                     /locus_tag="IVY61_000992"
     CDS             17954..18559
                     /locus_tag="IVY61_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7970121.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            18579..18941
                     /locus_tag="IVY61_000993"
     CDS             18579..18941
                     /locus_tag="IVY61_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970122.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            18944..19126
                     /locus_tag="IVY61_000994"
     CDS             18944..19126
                     /locus_tag="IVY61_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970123.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            19143..22562
                     /locus_tag="IVY61_000995"
     CDS             19143..22562
                     /locus_tag="IVY61_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7970124.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWIQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            22612..23349
                     /locus_tag="IVY61_000996"
     CDS             22612..23349
                     /locus_tag="IVY61_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7970125.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDAEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFSLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            23359..25650
                     /locus_tag="IVY61_000997"
     CDS             23359..25650
                     /locus_tag="IVY61_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970126.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNMIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHETRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVEDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVNNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQDGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKIA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            25674..>26751
                     /locus_tag="IVY61_000998"
     CDS             25674..>26751
                     /locus_tag="IVY61_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970127.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGEYDYSGNPNLSAKLNASSFS
                     LGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQYTISCEIMLEDGFTGDPS
                     GIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYTSDVPNEWYPLFRDIRTL
                     KPTGKVRLRKIKIEEGSTATPYQPN"
BASE COUNT         9667 a         3992 c         5736 g         7356 t
ORIGIN      
        1 attagcttac atatttaaag aaaaggagga aacatcatga aacaaaataa aaaaactcct
       61 tccgcaaaag gagctttaat aactttaaat tttaaagacc aaaagtttag tgctgttgtt
      121 gatgaaattc atccacaaga gcatcaaaaa aattcttttg caactgacga gaaaaagact
      181 cagaaacgct ctgagaaagt tcttcgaata attcaatttc tgttggaaaa gattcgtcat
      241 tttggaagtc tattttgaat tctttattaa cagtcttgaa tacggtttgt ttgaataagg
      301 cgtcgacatc catattaaat tcttccatac tattttcacc tcgctttcta ttcaaaatta
      361 tatcaaagaa aggaatgagc aaaaatgaac acaccgcaaa tttttaactt tgaacaacat
      421 gaagtaagaa cagtaacaat acatgatgaa ccattttttg ttggaaaaga tgtggctaaa
      481 gttctagggt accaaaatgg tagtagagat attaacagac acgtcgatgt agaagatcgg
      541 caaaactacc aaaacggtac ttttgaatcg cctagagggt taactattat taacgaatca
      601 ggtttgtact ccttaatttt aggaagcaaa caaccaaatg ctaagaaatt taaacgttgg
      661 gtgactagcg aagtgttgcc agcaattaga aaacatggag gttatctaac tccagaaaaa
      721 gtagaagaag ctttgcttaa tccagataca atcatccaat tagcaactaa gttgaaagaa
      781 gaacgtactg gaagactaat cgcagaacaa aaaattgcag aatacgaacc caaaatttcg
      841 tatttggata gtatcttatc ttctacagat tcagtaacta ttagtcaaat cgcggctgat
      901 tatgggatgt ctccacaaca gatgaacaaa ctacttcata agctaggtat tcagaaaaaa
      961 gtaggcaacc aatggttatt atgcaaaaaa cacatgagac agggttacac aaaatctcat
     1021 acaactgaga ttccgaaatc tgatggcgga acaaaagttg tgatgaatac aaaatggact
     1081 caaaaagggc gtctgttcat ttatgagtcg ctgaaaaaag aaggatatat tccagaaatc
     1141 gatttattag aggaggaatg acgatgtcac aagaatttat tttgaaagtc agaattcaat
     1201 tagcaaaata cggaaagtct cagaactggt tagctgatac tatcggaatt tctagaccat
     1261 atatgtcgga cattatgaat gggcgtagaa aaccagataa acaaatcaag ccaattgagg
     1321 cagcactagc agagttagag aaggagaaat aacatgcaaa taacaattcc agataattta
     1381 gtagtttccg aattaactac acagattacg aatgctgttc ttaattcatt ggacgaacga
     1441 ttacacctta tgaacaaatc agtagagctt cctccatatc caaacaaatc agaggtaaaa
     1501 aaagttttag gcattggtga tgacaaatta acacattgga taagcctagg cttaaaaaca
     1561 cagcagtgga gcaagttaga catcagaatt gaacgatcgg aactccaaag atttttgaaa
     1621 gaaaactttg agttctaaag gcaaaggaga atgattttat gtcctacaca ttgcaacaag
     1681 aacatcaaat tctaggtctg attaaacagc gtagaaaaca attacaagat gatcgtgcag
     1741 cgcttagaaa atccgatgag ctatcagata gacaagctga actaattgct tctgaacttg
     1801 aggatttgag aatgctagaa ataaaaaata gggagattag attatgaaga agacagacac
     1861 actttttata ggattcattt tggggttatt agtgattgta gcgcaccaaa gtattatcgg
     1921 aggaagcttg ttcgcagcat tgatggtttt aatcaatctg cttgactcaa aagaaaggag
     1981 caactatggc acgagaagaa gcgctaaaaa tcggtaaagt gattgctgat aattggtggg
     2041 caaatagccg ttctattatt ttaagcaagc aacatatcga aaagaaaaaa gcatggcaac
     2101 aaataaaaaa gtgactccgc cgaccaaagc aatgagtcac tcaaaaaatc ataactaagg
     2161 agattttaac atatggaaaa tgaactttcc actctagatc aatatttggc cgatcctgat
     2221 tggggcaaat cgaatttaca actggttgct aacaagtacg aaaaatcaat tgatgaagca
     2281 attgttgcat tgactgatgc agcttccata accgctgatg acagtaaatg gaccaagaga
     2341 gacttgggca ttctaaaacg aggcgttaat tggcttgaag atcagtacag agaagaaaca
     2401 aaagagaagt gatatgagtg tttaaaccac taatagattc atactcggcg gtactaaaga
     2461 aatttaaagg taacgacatt ggtgcaacaa tcaacgaaga agtaaatatc gaacggctga
     2521 aaacgatgta tgacggatat gatggcgatc gagtcattga aattcgcttt attgatccac
     2581 gacggttcac agtacagcaa agaaacttca tctatgcgct cataggcgat attttcatcg
     2641 atacaggcat gccaacggac ttctggaagg aattcttcta cttccgtttt gaaggtgtca
     2701 cagggcgcga aataagcctc aaagacgaat cgaatacaac cgtgagtgat gccaatatct
     2761 tagcgaatat catcctagat ttcatctttg aacatcatat tcctttcaaa gaaggatatg
     2821 agattttacc agcgaatcaa gagtattact tctacaaatg cattacaaaa agagtctgct
     2881 gcatctgtgg caaaacagga gctgatatcg atcactttga caaagcgctg ggaagacgaa
     2941 agcgcaaaga agttgatcat tcagagtaca catttgcagc actctgcaga atccatcaca
     3001 cagagaaaca caaaataggt gtgatcaatt tcaaaaataa atatcaaatc aaagggatca
     3061 agttaaacca ggaaacaatt aaaaagttaa ggataggagg ataaatttgg ctgagataag
     3121 ttggatcaaa cttaaaacta ctatgtttga cgatgaaaaa atacgattaa tccaagctgt
     3181 tcctgagtcg gatgccatca tcgttatatg gattcgatta ctagttttag caggaaagac
     3241 taacgacgat ggtctgatat atatccagag gaacatgcct tataccgaag aaatgcttgc
     3301 tacattgttt ggcaaaaacg taaatacggt tcgcttagcg ctaactacat tggcaaattt
     3361 caacatgatt gatctaagca gtgatggact aattgccatc agtaattggg aaaaacatca
     3421 aaatatcgag ggtatggata aagtaaggct aaaaaatgct gaaagaaacc gtaaatacag
     3481 ggaaagaaag agacaggaac gtctcaaatt ggaaaatgac gttagcgtga cgtcacgtga
     3541 cggtacagat aaagatatag aagaagataa agatatagat aaagaagaaa agaaaggtaa
     3601 gtattctgac gaacacttac gccttgctaa aaagttgcaa agtaatttaa ctgaagattt
     3661 tccaaaagaa atgaacaaag tagatatcga aaaatgggca gacacaatca ggttgatgga
     3721 agaaagagat aaagcatcta tagaagcgat tgagtatgtg atcaattggc tacctacaaa
     3781 tgaattttgg tttggaaata ttagaagtgc taagaaattg agagaaaaat ttgagaagct
     3841 caaattcgaa atcaaagcag acaagaagaa tcataaaaag caaagtcaaa aactacagta
     3901 cagcaatcct agtgaatatg acgacttgcc aatttaaaaa ggagatgcat cacatggaaa
     3961 gcctagcaaa tgctatggag aaactaataa gaagagtatt agtgcaaagc ggaaaatgtc
     4021 cagaatgtag cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt
     4081 gtaaaccaac atgcatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga
     4141 ccgaacggat atataacgat agcttaaaag cacgagcgtt gagttttttt caaaatggtt
     4201 cggtattaac agataaaact ttgtttaaat gcaaaatgga aaattatcac gtagtggatc
     4261 aagaaacaaa aatcgcttta gaaaaagcaa aaagctatac gaatgaggtt ctgctgaacc
     4321 atcctgcaca tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg
     4381 ccacagcttg ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct
     4441 atcaagaatt attagagcaa ataaagtttt cttataacaa tactgaactg agaaaagaaa
     4501 ttgaaggatc gcttatagcc gatattaaaa caactgattt ggtggttttt gacgatattg
     4561 gagctgaatt aggtagtggg gtatcaaata gtaggcagtt tacaaacaac acgttaaaca
     4621 cgctcttaga agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg
     4681 aactaagaga agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt
     4741 atgcgctgaa attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg
     4801 catgaataaa taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc
     4861 agaagcagag tattacgatc tagccttgtg gcaagctgaa gcaaatggct ggaaagtaaa
     4921 acttcaggaa agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa
     4981 gatcgagtat attcccgact tcacatttta taaaaacggc aaacttgtca aagtcgtaga
     5041 tgtcaaagga atgcagacaa aagactttaa gatcaaggca aagttgttct gtcatcaata
     5101 tcaagcgccg ttgatattag ctaaaaaata tcggaatacg ttcaaggaag agcgttttta
     5161 acgaggtggt ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aaacattcag
     5221 cgtgaaattg acgatctaga acgaacaaag gcagtgatgg tcaatgaaac ggcgagaaag
     5281 gcaatcgatt tgcacataga gaatttaaga agggaaattc gtagattgga ggaataaacg
     5341 tggataagaa agcaacaatg aaacgaattg ttgaattaac caagtcagaa tcttggcaag
     5401 aagacaaaga aatagttgca gaagtccaaa agctcggtaa atcaatgtgg actgaaaagc
     5461 ctaaacggaa aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag
     5521 gtacagctga acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag
     5581 ctaggagctt atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaaaaat
     5641 gctagacatg agaatcgaag attatcgaat taccagtgat tctagaaaca ttgtcctatc
     5701 aaaggtaaga cgagatgagg aaggaaatat ccgctacaca gaagcaaaag aagaatcacg
     5761 agcagatatc ggatacttcc aaactgtctc atcgtgtttg aaggcgatac aacgcgatta
     5821 cgtgttaagt gaagaaagaa cgataaaaag tattatcgag tacaaaaaag cgttagaaaa
     5881 catcactaga cagtttgaac aggcatgtga gattgaggag gagaaataat ggaattcgta
     5941 gacttaaatg tacgtgggat caaatgcgat aatccagaat gtgattattc tgatatggca
     6001 gtaaaatacg aaaattatcc gcaatggttg aacaagccat gtccgaaatg cggcgctaat
     6061 cttctgacgc aagaagactt ggatgcaaca gagcagctta tggagattgt caatcttaca
     6121 aacgagatcc tcaaagattc aggattggag aagcaggata tgaataaata tatcgtacct
     6181 gtagaagcga atggaaccgg tgaattgtca tttggagaaa taaaaaaact ggaggaagaa
     6241 aaatgaacga aaataaatta atcaaactag gtgtggcagg agtagtaata gtaggtattg
     6301 gagttatcgg aggatttaag tttttcgaaa aaattgataa cggatatgtg ggtgtgcgct
     6361 attcaatgaa tggcggtatc aaagatgaag cactgacgca aggtgtgaaa tttgttggga
     6421 ttgacaaagt gatccaatat ccaattcgct tgcaaactat ccaatcaaaa aacatttcag
     6481 tatcaacaag cgatggcaaa aagacaacga ttgatatcaa atatgactac aaagttgatt
     6541 caactaaagc agcaaaaatg tacaaagaat ttgggaatat aacttcggaa gatatcgaaa
     6601 gtggatggtt aaaatctaag cttcaaaaag tagcgcgtga agtttatgcc aaatatagcc
     6661 tgcttgatgt cctttcagga gattcctcta aagttgaagc tgaggtacta acgaactttg
     6721 ctaaatcagt tgaatctaaa gggtttgaag tcgaagacgt aacacttggt gttccagatg
     6781 tcgataaaga aacacaaaaa tcaattgatg cgatcattcg agctggtcaa gaaaatgaaa
     6841 aagcgaagct agatgcagaa actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa
     6901 caaaagctgc agaagcggaa gctgaatcaa accgtaaagt tgccgaatct gtaacagata
     6961 atttaattcg ttatgaagaa gctcaagctc gaaaagagca tggatgggta acagtaaacg
     7021 gagcagatac tgtagttacg gatgaaacag gtaaataata tgggattctt tatagctaaa
     7081 attctcttgt tcttaggttt ggtaggagtg gcatatctcg tgtatgccct cctttccaaa
     7141 actgatgaca aagaagatga caacgacgat gaaatgaaat tttaggagga gaaataatgg
     7201 atctcattac acaatacagt gaaatcatcc tcaagaaaat catgatgaag attcagaaag
     7261 ataaaaaatc aaaagaacga gcggaattag ttaagttgga aatggctgaa acaggagcag
     7321 gagtgcgaag tagcaggcat tggaaagcag cagcaaacat tgaattctat tacaacgaaa
     7381 ttcaaaaagg gttcgatcag atgcgtgagc tggatcggca aacaaattgg agcaagaaac
     7441 ttcatcaaga tcgtttcaaa tttgtagaaa agtatagaga gatactgaat gagtatttcg
     7501 aggaggacta gatgtttaat ttaatgttag cgtcaattca ctttttattc tatataacga
     7561 tatttgtagc tatattaatt aatacgataa aaagcggttt tttaacacca atcggcacaa
     7621 ttttaacatt agcttcattt gttacaagtt gtattatcca acttaaatat ataaaggaga
     7681 atgaataatg gacgaactaa tcacaaaagt agagcagtgg gctaaagata agggattgga
     7741 tcaagctgat tccagcaagc aaatgttaaa aacgatcgaa gagattgggg aagttgccgc
     7801 ttctctagct agaaaagatg aacatggttt aagagacgga attggagacg tagtagtaac
     7861 cttgattatt ttagctatgc aaaatgatat ggatttgtac gagtgtctga accaagcata
     7921 caacgaaatc aaaggacgca cagggaaaat ggtagatggt gtattcgtga agtcgagtga
     7981 tttggaggaa gcggaatgaa caacagacat cgcagaataa caaaactaag aaaacaggaa
     8041 ctgaatgtac taaagacaaa gtttgaaaaa gaatatggaa tttcagcaga agaaacatat
     8101 aaagtggcaa gtcagtgtgt tgctgatgca agtgatgcta ttcgtaagtt tggaatttcg
     8161 atattaagtg atgatcgtaa atgggaggaa aaagaatgaa actaaaagac ggattttacg
     8221 ctagcagtca cggtatcggc ggtttaatgc tagatatgcc gacaaagaat cctaaaacac
     8281 gtaagaaacc aaaagtcaaa gtcggtgaca tggtccgctg tgaagcagag gagttcattt
     8341 atccatttcg tggatatgta gaacacgtct ataatcactc agcgatcatt cgcattgaaa
     8401 acacgatgga atgtgataaa tggacagcta aaagcaaaga gaatttagca gtagtgagat
     8461 tggtggatat ggaactaatc aatgacaaat aaaaaagccg gatcgctccg actaacataa
     8521 taaaacagac aagtttatta tatcacataa aaggagcagt ttgacttgat gcaattgtta
     8581 cgagaggtag atttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg
     8641 cgtttggagc ggatggcagg tcgctctttg atagatatta agtcgccgat tattacggat
     8701 atgccgaagg caccgaagca cggcaataag gcagaggatg cattgattca gatgatggat
     8761 atagaagcgg agagagacgc gattttagcg gctttgatgg ctcttagtct gattagtcgt
     8821 cagatactct actatagctt ctgtgacgta aataagcact ctaattatga aataggtcaa
     8881 ttgatacgag gatacggaga gaagaatgta gagaagctga aatctatcgc gctgatcgaa
     8941 tttgctgaag catacaaaaa aggcgtgtta gttcagtatc gttgattttg tagggttttt
     9001 gtagggatag tgtagggttt ttgagtgttt taacgtgata ttatgatagt gtcgaaagat
     9061 taggaaacag gatcgacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     9121 agcttcgata gacagcagcg gaaatattaa gaataaggat gtgaatttta actccttcta
     9181 aattgttctt attatctatc atccgttgct gtctattgtc attatgtcac tgtggcggaa
     9241 agggtagacg cttaaaaata aggtcaatac gtcgagggat agccttaacg ttttatgatt
     9301 tgaccatgca atgttcgatt cattgccagt gacttaagga acctacggaa acaattcatc
     9361 ttatcggatg ccgatgaatt ggctgactag tcgggatgct actagcagtt agaaggcata
     9421 aaatactagc gcagacgtgc gccactctca ggtgtaggtt aggagagaaa cattagttgg
     9481 ggttattagg aatacgataa cctgcttgcg acaaagcttt gtactgtcgc gttggtcatg
     9541 aacagagacg gtattctgtt tcagtattcg ttagcaaccg agggatgtgg cagtggtgag
     9601 gtgcaggaag tatcagactt gtttgtgtgc aagttactat tacatattag atcactcttt
     9661 gagtggtctt tttattttgc gtaaaggagg cacgacgatg tatagaccac aatacttaga
     9721 acagaagcat gaagtaatca ctgtgcaaaa cggtaacggt gagatagtac gaaagtatag
     9781 aagaccaata aagagcgata catataaacg gaaggaaagc aatgaagtta ttccattgta
     9841 tggcaaaaga atagctaagt attaaataag attgcgaaag gagatgggac atgaccgagg
     9901 aattctatag atggttatta cagttgataa gagaagatcg tttggttaag ttctatcagt
     9961 ctcctaaatg gcgcaggctt agagagaaag cgatgaaacg agatcactat gaatgccaag
    10021 agtgtagaag actaggtaag tatcatagag tagagaacgt tcatcatata aaggaagtca
    10081 aggatagacc tgacttagct ttagatttag ataatcttat ttgtttatgt gttgaacatc
    10141 ataatgaagt tcatggcaga tatcttacag cgttagataa acaagagaag aagatagaaa
    10201 gcttcgctaa cttcgatgca agtgaaaggt ggtaagtgca tgatcatcaa tgacaatggc
    10261 agagagtatg atacagaaaa gattgaagag tattcatctt atactcaggg attaattaaa
    10321 cgtttgatat acgttcgcta tgtaggtatt agggatctgt tatcagataa ctgttgtagt
    10381 aaatacaaag tgaatcaagt aagagaagcg ttgaataaag ataataacgt cgaaagaata
    10441 aaaaatgttt ttggatatag tattgaagag attaattatt acattgactt cgctgaagct
    10501 ttcattccga tggtgagata acccccccct taaaataaat cgcaaatttt ttgggggtga
    10561 tgaaacggag ggggctgtca ggaaaagaga ttttttcgaa ctttatcatg aaaggagggc
    10621 taaaatgttt aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac gccgctcttt
    10681 aataagccaa ttggaaagtc agaaaacaaa tattgagcca ttcttagata atgttgatcg
    10741 ttatatcagt ttatgggaaa cggcgatatc actggaagaa gatatatccg agaacggcat
    10801 tagactggag aatggtaaaa agaatgaatc agtagcgttg cttgtttctg tcaacaaaca
    10861 aatgggattg atgttggata aacttgccat tactcctgaa ttggtaggtg aagcaaatga
    10921 atcaattcct gagttataag catattgaaa attggttcaa agctatagaa gaaggcacta
    10981 tcaaggtatg caaagagcaa ttattgctaa aaaattatct agaagaaaga gtctttacta
    11041 gagaagatat ttacttcgat aagcagatgg tagaggattc aatcaatata ccagcacaat
    11101 actttccatt cgaattaatt ccgtgggaaa aatttctaca atgttttatt tatggtgttc
    11161 gatggaaaaa agataaaaca ctagtgttca atagatatct ttcattaatg ggacgtggta
    11221 atggtaaaac tggttttgct tcttggaaca acttctttct actaaccgct aaacacggta
    11281 ttaaaaatta tgatattgat atctatgcca ataatgaaag ccaagcaaag actagttttg
    11341 atgatgtatt taaagtaatt aaagatcatc ctgatttaga taaaaaagta tttaaagcta
    11401 cgaaggaagt tattcaaaat atcgctacaa acagcaaact tcgttataac acggcaaatg
    11461 ctagaacaaa agatgggaag cgaccaggtg caaaccgctt tgatgaaatt cacgaaaatg
    11521 aagattattc aatgataaat gtggctactt ctggtggtgg taaaattcga gattatagag
    11581 aattttatga tacaactaat ggtcatgttc gtggtggtcc gcttgatgac attatagaag
    11641 aatcaaaaat gattctttct ggagaacttg gaattgacaa ggatggagca gaattttcta
    11701 gtttgtttcc atttatttgt cgcttggata acgataatga agttgatgat cccgacatgt
    11761 gggaaaaagc ttgtccaact attaattaca atgcagattt aaaacggaaa atgtttcaag
    11821 aatactctca aatgcaacgt aatgctggtt taagacttac gttcatgacc aaacgaatga
    11881 acagacctat ggaagataca cgatttgctg ttgcttcata tgatgatgtt ctgcatacga
    11941 aagaaaaaga atttcctgaa aaaatggatg aagtgatagg aacagtcgat tttgctgata
    12001 gacgagattt tgccagcgtt gggttgctag gaaaatacga taaagatgtt tattttacac
    12061 aacatacttt tatccacgaa tcagcccttc gattacaaaa catcaaacga gaggttatag
    12121 atatttctat agatcaagga aaatcacaga tcgttcatgg aaaaaatata gaagctgatt
    12181 atattgtagg ttggtttctt gaaatgagta ataaatatta tattaaaaaa atcgctatgg
    12241 atatgtaccg tgcaaaaata ttgaagcccg ctttagaaga agcaggtttt actgtggaaa
    12301 ttgttcgaag cggatctgtt acacatggta tgttaaaaga tctggttgat gaccttttta
    12361 ttaatcaacg tttatttttt ggtgacgatg cgattatgcg ttggtattgc atgaatgtat
    12421 atgaagagca tatttctaat ggaaatatac gctatgaaaa aatagaacct gaaactagaa
    12481 aaacggatgg ctttttttca ttccttcatg gtttgaattt tttagatgat atttatgatt
    12541 ctgctcctgt aacagtcaca aatagctcag tagaaaatac aggaactgga tttactcctc
    12601 tagtattcta acttgaaagg aggtgagaaa gtggggattt ttcaaaaggc ggtaggatac
    12661 ttcacaaaaa aagcaacggt tcctttagaa gaatactttt gtaaattgca agttgatttt
    12721 gtgtatcgaa aatttgcgat tgaaacttgt attgatttga ttgcaaatgc gatgagtaaa
    12781 gcggaattca agtcatatga agatggaaaa aataaaaaga atgatcttta ctataggctg
    12841 aatgtagctc ctaataagaa aaataatgca acagaattta gaaaaaaact gatcaggaga
    12901 ttaatattct acaatgaagt attgatcgtt tctccgtcta ataattctag cgaaatattt
    12961 attgcggata gttgggatgt cacagaatat gcattgaaag atgatgtgtt ttctcaagtg
    13021 caaattaaca acatagtcct tgatagagaa tttctagaaa gtgatgttat ctatataaaa
    13081 tacgcagatc aacaaattag gcaactagtc gatgcgtatt atcaagcgta tgggaaactc
    13141 atttctagtg ccatgaatgt ttacaagcgc tctaacgctc gtagatacgt actgaaaggg
    13201 aatttattcc gaccgcaaga caatacaaca caagatcaaa tcaataaaat gatgacatca
    13261 caatttaagg cttttatgga agctgataat gcaggtgcgg tatttcaatt acaaaatgag
    13321 tacacattag aagatttcag cggaaacttc caaagcaatt caagagatat aaaaaactta
    13381 atagacgaca tctttgagat gacagcagca gcgtttcacg ttccgaaaaa cctactaaag
    13441 ggagacatga gtgggttatc ggatcaagtg gacgcttttt taatgttcga aatcataccg
    13501 attgctgaac ttattcagga tgcgtttaac gctagtctct atgaagtaga agaatacttg
    13561 tcagggaatt ttgtacgtgt ggatacaact atgatcaaga ttactagctt caaagatttg
    13621 gttgacgcta ttgatgtagg cattagaaat ggagtattta caatcaacga aggaagagag
    13681 cgcgttggaa atgatcgctc tgataaggcg atggcagatg aaatatttat aactaaaaac
    13741 aatcaacaag tatcgaaagg aggtgaggcg aatgacgaca atgaaaacat ttctagcagt
    13801 aaagaatgaa ggcgcagtac cgcaaatttt tattcaggga tttattggtt ctagttggtt
    13861 ctttgaaggg aatactgaca agggaatcaa aaatattttg gatagtctag gtgatcaaga
    13921 agaaattgaa gtagtaatta attcaaacgg tggagacgta tttcaaggga ttgctattgg
    13981 gaacttactt aagtcaaata aagcaaaagt taacgttgtg attaacggct tagccgctag
    14041 tgctgcttca attatcgcaa tggctggcga tactataaaa atctacaaca atgcacaatt
    14101 gatgattcac cgcgcttcca catacggaga aggaaatgtc gatgacttcc gtacgattgc
    14161 tgaccaactg gaatcaattg ataaatcggt aaaggcttca tataaaacac gattcaatgg
    14221 cacagatgaa gcattgcaag aacttcttga aaaagaatcg tttatggatg cagaaacagc
    14281 tttgagttat ggattggtcg atgaaattat cgatgcagaa aatagctcag gtactgaagc
    14341 taaaaaagaa caaagcgttg aagaaatttt gaatgacgtt aaagaaaaaa gagcagaaaa
    14401 aattgctgca tttacagcag cattaaataa aacatttgga caaggagatg taaaataatg
    14461 acagttaaaa atttaaaagg tgtaacagct gcaagcgacc aattgatgaa agcttttaaa
    14521 gatggtaacg aagaatcttt tagcgcagct atggtaagct tatctaagga aattcaggat
    14581 aaaattttag aagaagcaac agcaaaaaat caagatcaat tagtattaat gaaccgtggt
    14641 cagcgtgtat taactacaca agaaacaaaa ttctataacg aagtagtgaa aaacgaaggt
    14701 tttgcagggg tcgaagaatt agtgccagct actgtatttg aacgtgtatt tgaagattta
    14761 gaacaatctc atccactatt gcaaaaaatt acttttgtta acacaactgg tgtaacagaa
    14821 tggattgtgt cacgtggagt caatccagca tggtggggta aactttgcga agctgttaaa
    14881 aaagttttag ataatggctt tgatgtaatt aacatgaagc agttcaagct atcaggttat
    14941 attcctgtat gtaaggcaat gcttgattta ggtccagtat ggttagatcg ttatgtccgt
    15001 actgttttag tagaatcatt gagaattgca ttagaacaag caattgttga tggtactggt
    15061 aaagatatgc cagtcggaat gatgcgtgac atgagcaaac aaactagcgg agaatatgct
    15121 gaaaaagaag cagaacctat tacagcttta gatgctgtaa ctatgggcgg tttgatggcg
    15181 cgactatcaa aattcaatat cgaaggtgtg aatgatccga tttatcgtaa tgtgaatcct
    15241 tctgatgtgg tcctaattgt gaatccaact gattactggt ctaaagtatt cccagctaag
    15301 actgtactaa ctgctaatgg agaatacgta caagtattgc cagtaccagt ttcagatttg
    15361 cagtcaacgg ctgtgccaga aggaaaagca gttattgggg tagcctcaga ttacttcatg
    15421 ggtgtaggat ctacactaaa aattgaagct tcagatgaat accattttgt tgaagacgaa
    15481 cgcatttatc tagctaaaca atatgcaaac ggtcaaccta aacgtaacga tagtttcatt
    15541 gtattagata ttagcgcttt gggaactact actacaacta caaaaccaac aaccacaaca
    15601 actacaacac aagcgtaggt gatcagaatg aagtatattc tttgtcagcc ggcaatcaat
    15661 cggtttaaat gggagcttga agtttgttta actaatctga agaaactagg aatcaaagat
    15721 atcgtattgc ttttcagcag acacgatgat cagattccta ttttttttga gaaggaatat
    15781 ggtgttgaag ttcatgtgta cgatgatctg cgggacgaca aagagtatat tccttcgatt
    15841 aaaccatatt tatggtggaa atatttagaa gaagatcatt cgcgtgagga cgaccgatat
    15901 ttctatatcg attcggatgt cattttcaat aaaagaatta atttgcgcaa attgccttct
    15961 aaagatgatg tttggtattg tagcgactgc tgtagttatc taagtcttga ttatattaga
    16021 agctgtgaaa acggagaaaa tattctaaaa gatatggcaa acattgtaaa tgttacagta
    16081 gaatctttgg aaactataaa cactaattca ggaggcgcac agtgggttat taaccgccct
    16141 aaagcgaatt attggaaaaa ggtttatctg gattctaatc ggctatatcg ctaccttaga
    16201 gggcaaaaaa caaatataaa aatctggaca gccgagatgt gggcacagct ttggaacatg
    16261 atgtatttca atattggtcc taaagttcac gaggaattag acttttgttt tgctactgat
    16321 ccaatagaaa aagttaaaga agtaaaaatc ttacacaatg ctggagtaac aacaaacgat
    16381 gaagatttat ttttcaaagg gagatacgtg acttctacgc cttttgatga agatttatca
    16441 tttgtaaaca agaaaaaatg ctcttacgca tatgttaaag caattaaggc ggtggttaga
    16501 tgacgcctga acaagtgact gaagaattgc taatagctgt gaaggataat atttacgtta
    16561 cctggaacga agaagatgag tcaattaaaa agatgatagc taaaaatgct gtttatcttc
    16621 aaagtaaagt gagtacaaca ctttcttttt ctcctgaaag cttagaatac ggattgctaa
    16681 tcgaaagatg tagatacgac tggaatcgtg ctttagatga gtttgaacaa aatttcgcta
    16741 gtgagttatt aggtttcatt caacattatg cgctacaaga atatattgca ggtgatgtga
    16801 atggcgaata atcgtagact cgaagaaaca ttcaacgatg gttggttaaa gattttgacg
    16861 caaactacca aaagaaatga actaggaaaa aagattggtg tagaagatac agaaatcact
    16921 tctttaaaat ttagaaatct ttccatgaga gatagtgata taacagctat ggatgcgatg
    16981 ggatcgaaat taactaagaa agtaaagact ccatttcatc caatcgccaa gaaatttaat
    17041 aaagatcaat attttatcgt aatcgatagt atgcgttaca acgttatcta tgccgattac
    17101 gataattttt atatctattt ttatcttgaa agtgtgggtg aatatggtga ttgataattc
    17161 taaagaaaaa gaacgtttaa ataagcaaat ttctgctatc aaaacttcct tagaagagca
    17221 ttttggcctc aaactctttc aagactccgt aggcgaggat gagctacctg atgattttaa
    17281 ttacttcatt ctcgaaacag gagaaataga aatgatcact gagccaaaat atagcgtggg
    17341 tcaaaatcta tatctaactt tctattcaga aaatagagaa gatttaacag gagattcact
    17401 agatattatt tcattgattc aaaatcgttc gattcgtttt cagagaatgg atcccaatca
    17461 tttaaaacta gagaaccaag atcgctatat cgatcaattg gtatttacgt ttagacgatt
    17521 attgaagagt gattgtcatg gctaaaaata gttgggagct aaaaataaat ggacatgatg
    17581 aacttcttgt gcggatggaa cgctattcaa gcgagagcga acgactgatt aacgaagcat
    17641 tgaaatcaaa aggttcggct attgcagtgg ataggattac agaaaaaatt cctgtttctg
    17701 aagcagattt aagaagaggg caccaacacg caaaaaatag tcgtccactt aagactcaat
    17761 acattaattt gggtttcatc attagaccta caagaaaatt tgagtattta aaatatcctg
    17821 atttggggat aggtacttct aaaagaaatc agccggacga atttatgaga agaggattag
    17881 gtcttgcact tgatccaatt acagaacttc tgattcgtca attcgataaa ttaaataaat
    17941 aggaggaaca acaatggcta aaacaacaac agtaacaact tttgacaata taagtattaa
    18001 aagaatttct tttaatttta agaatgcaac aaatgcaatt tcaactgatt gtaatggaca
    18061 attagatggc gaaacagaaa tgcagagaat tgtaaaaaaa tgcggttcaa cagaagtaaa
    18121 atcgaaatct aaaccaatca atatgacggt aacaattact gcacatgtac cgatggaagt
    18181 ttatcgacgc ttcaatgggt tgaaacaaga tgaacgtatt aaatcgggaa tttactctta
    18241 tggtcctgat tccgtagggg aagatttctc tcttgctgca gagatcgtgg atgatttcga
    18301 agaaaaaaat aagctgattg gtatgttagc atgcacttcg aatacaggat taacattctc
    18361 tattgaaaat ggtgcggatg aagtagctgc gttagaacta gaaacaaaag ttatgcaaga
    18421 tgaatttggt aaattttatc atgaagcaat tgttgcagaa cttgaagaag acttaacaga
    18481 tcaatggatg acgaatctat ctgctgatgt gattaaaaaa acttcagttg tgacaactac
    18541 ggccactcaa tcacagtaaa aaaaacggag gtagcgaaat gaacgaagat tactcaaaaa
    18601 ttgaactaaa cgatggaaca attttgaatt tagaacctaa actgaatatc aagaaattat
    18661 tgatgatcaa tagagatttt aacacagacg agtttgcaaa aatgactgtg ggaaaaggat
    18721 ccatggatat ttctgttatt caaggtgcaa aggctgtgta tattgcttac cgccaagcga
    18781 acatgactga ttatatttca ttcgatgaat ttatcgataa atgggatttt gatatggcta
    18841 ctgccagcta tatttatcaa ttgatgatgt tcaaacaagc acgcgatgct tatcaaaaag
    18901 aatttgaaaa agcaaataag gaaaaaaagc ttcaaaagta aaaatgccaa agctcttagt
    18961 tgaaacgtgg gtcgatgtct attcgatgtt gaccgacgtt ttttctatgc cttcagattt
    19021 ggttttaagc gatatctgtt tagatgacat tttgcaaatg gcttacaaca agagtgctta
    19081 tgaaggatgg aaaaactatg caataaacca atcccagaaa aactaaagaa aggaggtaaa
    19141 aaatggctaa aaagagaaca gaagcagaag taactttcat agctaacgat gacggattga
    19201 aatctacgtt aaaagaaatc agtgctgaat taactaaaaa tagagcagaa ttaaaactag
    19261 aacaagctca attacaacag actggttctg aatcagacaa gttaggaagt aaattatctt
    19321 ctttagaaaa gcagtatgaa ttacaaagtc aaaaagttga agtaactagc caacgtttag
    19381 ccaatgcgaa aaaatattat ggagaaaatt ccaccgaagt tcagaaactt gaaagagaac
    19441 tgattaatca acaaacagcg caacaacgtt tgtcaaacga aattgataaa acgagtaatg
    19501 cactagctca agcaaaaggc gaaatacaga cgtacgagtc tacaatgcaa cagttggata
    19561 gcgaacaaaa aaatgttcaa gctagtgctt ctctgattga atccgaatac aaaaaatggc
    19621 aagcaactgc tggtcaatca gcttctgaag ccgagaaatt agcgaaagcc caagaatatg
    19681 tttctcaaca atctgaaaat gcggagaaaa cgatagatat cctaagacga cagttagaag
    19741 ctacacagtc tgaatttggc gctacatcca cagaagcaat gcagatggaa gcgaagctta
    19801 atgatgctga acgtgaattt gaagagttag gacaagctgc taaaaatgta gatacaacta
    19861 acttggacga tatcggaagc aaaatagata tgaataattt aatggaagct tctgacgttt
    19921 taagcgacat tggcgataag cttacagaat tagggaaaca agcagtggac tctgctaaca
    19981 gtgtaggtag ttcccagagt aagatacaag ctaattttgg tttgtctaaa caagaggctg
    20041 aagaattaac gaatgtagcc agagacattt attataaagg ttttggagaa tcgttagatc
    20101 agtccacaga tgcattgatt ttggtaaagc gtaatttagg agatttaaat aatcaagatt
    20161 tacaaaatat cacggaacaa gctatggtcc tagaaaacac catgggcgct gatatggatg
    20221 aaacgttacg tggtgtaaat ggcttaatgg tcaatttcgg cttgagtgct caagatgcaa
    20281 tggatttaat ggtttcgggt actcaaaacg gtttagataa aacgcacgaa ttaggcgaca
    20341 atatggcaga atatagccaa ttatggagtc aaatgggata ttcagctgat gaaacgttcg
    20401 gaatgcttca aaatggttta gatgcgggtg cttataacct tgataaagtc aatgacttag
    20461 ttaaggaaat gggaatatcg ttaacagatg gtcgatttga gcaaaacatg gatatgttta
    20521 gtgaaagtac tagaaaagct tttgaagagt ggaaaaatgg cggaggaaca caaaaagacg
    20581 ttattaattc catgattcaa gattttagca atatggatgg tcaatacgac caattaaata
    20641 aagcttctac aatttggtct gcacttggcg aagataacgc gatgaaagtt gtccaatctt
    20701 tgactgatgt taatcataca tttgatgatg ttagtggatc tgcacaaaaa atgaatgaag
    20761 attctactac cccgttgcaa gagttgaacg gtaaaatagc tgaattaaag gattcattag
    20821 ctcctatagg caacacaatc atagatgcac tcgaaccagt aattgatttt ctaggaaaga
    20881 tggctgatgc gtttaataat cttccacaac cagtacagga ttatgccgta gcgattggcg
    20941 gattgactgc tgcatttact ttattaatgc caataatagt tggcttcatg gctctaggtg
    21001 gtcctactac attaataata ggagcagtta ttactgctat tgctggagtt atagcaatta
    21061 taaaaaactg gggcgcaatt actgactggt ttaagggaat atggagtaaa ttcactgatt
    21121 ggttgggtgg tacttgggaa agtataaaag aaggtgcctc atcagtttgg gatggagtca
    21181 aagaaacctg gtctggattt gtagattgga ttcaagatat ttggcaaggc gtttctgatt
    21241 ggtttggaga gctgtggagc ggattagttg aaggagcttc caacatctgg caaggagtcc
    21301 aagagacttg gcaaacattc gttgattggg tttcaaatat ttggaacgga gtcaaagaag
    21361 tatggtcgat tatttgggca gatattgtag ggattgttca aataccatgg accttaataa
    21421 cgtcattgat tcaagctggt attaatatta tagtgggtat ttttgatgta gctggacagt
    21481 tattaggcgc agcttggcaa gctgtttgga cacctatttc tgatttcctt aaaaacactt
    21541 gggatactat gacacaatgg gtaagcatcg cttggaatgg aattgtaact acattccata
    21601 ctatatttga tccagttgtg gcatggtgga acggtatatg gacatccatt agtactacgg
    21661 cttcaaatat ttggaattca attagtgcaa cagcttctag tatttggaac agtatcaaga
    21721 atacaatcac tagtttggta caagcagctg ctacagtaat tcaaaatatt tggtcaactg
    21781 tatctagttg gttaggtgga atttggaatt caatcagctc tacagcatca aatatctgga
    21841 atagtgtgac tagtagtata agcaatgcta taaacgcagc taaaagtgcc attcaaagtg
    21901 tttggaatag tatatcttcg tggatcagcg gaatttggaa cggtatcaaa aacactgctt
    21961 tgaatctttg gaatggaatt acaagcacta ttagctctaa agtaaacgat ggaaaaaatg
    22021 caatttcaag cggttggtcc aatctaacag gtattgtttc cgacatattc aataatgtta
    22081 aaagtacaat tgctaacatt tgggaaggta tcaaaaagac tgttagcgct ccgattgatt
    22141 ggattagaga taaaatcagt ggtatttttg ataatttgaa tatttctata ccacatattc
    22201 cgttaccaca ttttaaattg agtggggaat tcaatccatt gaagggaaaa atcccaacgt
    22261 tgggtgttga ttggtatgcg aaaggtagtg tgtttaattc tccgaatatt atcggtgtag
    22321 gtgaagcagg acctgaagca gttttaccat tgaaaagatc tgtgctgcaa gaaattggtg
    22381 atcgtatctt gagtagcaca tcagtttcat ctagggcaca aacgattcaa cctgtgaaca
    22441 attacgaatt caatttcaca attgatggta acgcagatga ggttactatg aagcaaacaa
    22501 ctcaacaaat cattgacagc attacaaaag ttcaaaatga taatgcttcg gcatggcgtt
    22561 aaacaggaga gtatttctcc tgttttttag tattaaaaag gatgtgaaaa aatgactgat
    22621 tgtatacatt ctataatcga tggatttcct gattatttgc ataaattggc tttagctgaa
    22681 agaccaacca taccatctcc aaaaagacag agagttgaaa cttctgtttt aggacggtta
    22741 ggtggcttag tacaagatta ttcgtttgaa gatatgtcgt ttactttgca ctataactat
    22801 ttagaggatg cggaagacca tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg
    22861 ttaaactatg caaagaaatt agaattctct gatgatccca acgtctatta tgttatccag
    22921 actatcgata ttggggatgc agaaaacgat attgttgaat ggggagagtt cgatgtaaat
    22981 attactgcga aaccattcgc aagagttcaa gaagatgtgc ctataaccgt agataaacca
    23041 cagtcattta gtttgctgaa taatagttta gaagaaagtt ttccaaagat tatcatcact
    23101 ccttcagcta cttcatgcca gttcatctta aatgattatg tgtttagttt tgaaggctta
    23161 gtagtaggaa ctgatgtagt cattgatagt gatttaatgc tttgctacga agagcaatcg
    23221 gacggagata ttttagatcg atccaacaaa atgaagacca tgcaatatcc gacattgcaa
    23281 gtggatatta attattttaa ttgtactggt ttgagcaaaa tacaaattta tcgtaatggg
    23341 ttaaggtagg tgaaatagat gatcgataat ttaataacta tttacgataa aaatgacgcg
    23401 aataatttag ctgaacattt atatgataca caaggtttag gcgctttgtc agactggtta
    23461 acagccactg ttagcaataa attaaacgga gccgagatat ttcagggtac ttatccaata
    23521 agcggaacta atgcagactt gattgtagac ggacgtatta ttcagtgcta tgtggatgaa
    23581 aatcgagcaa agcaacgttt acggatttat tatgcaaaga cttctgtaat aggaaatatg
    23641 atagaagtaa aagctgaacc tattttcaat gatataagaa aatcggtgtt gaataaatat
    23701 gacagcggaa cagaaaagat cactgctact caggcatggc aaaacgcaaa agttttagca
    23761 aaaccagcta ttccttcgca gttttctttc tcatcgttag tagatacgct tgctaatgtg
    23821 aagatagaaa aggcgaattt tttagaattc tttggcggaa aggaaggatc tatcctagat
    23881 cgatttcatg gggaatttct taaagataat aacacattac gtcatgaaac aaggctaggt
    23941 acggatcata aaatcaaagc gatttatact aaaaacttaa ctggtctcga cttagagata
    24001 gatgctcaaa gtgttttagt tggagtttat ccattcatta gcagttcgtc agaaggagag
    24061 gatgagatca ctctaccaga agaagtcatt ttcacggatt acgtggaaga ttatcctgct
    24121 ggatatgttt cttttgttga ttttaaagac aaagcgactg atgtggccac attaagggaa
    24181 gctgctaaag actggttgaa aacaaacata gataaacaga aaccacaagt gagtggttcg
    24241 attgaattag taccattgag gcatcaaaga ggctatgaaa aatttgttga tctagaaaaa
    24301 gtttcgatgg gtgacggagt agatgtgtat catccacagt taaaagtgaa tatgtcagca
    24361 agaattgtgg aatatacgtt taatgtttta accaattcat acgataaatt agttgtagga
    24421 aacgtcaaaa caaacttctt agaaaataca gagaataatg tcaacaattt gattaatgat
    24481 gccattgatc aattgaaaaa tggtggcgaa atcagtgatt taatcaatga tattgtagat
    24541 catcaaactg acatgattac tggtcaagat ggtgggtatg ttttactaga tcctaaagaa
    24601 gcacctagtc gtattttgat tatggacacg ccagataaga ataccgcaag gaatgtttta
    24661 caaatcaaca atgctggtat tggtttctct aaaactggta ttaatggaac gtatgacacc
    24721 gcatggacgt tagatggcgg atttaatgcc tcgtttatta cagctggtga gatagtaggg
    24781 attactatta gaggtactac attaattagt gatggtactg attatagaac aagtattgcc
    24841 aatggcaaaa tgacttggta ctcaaaaaaa gttaacaaag atattatgga gctagaagca
    24901 cgtgattatg taagtgctga tgccggtatt gtatcataca ccatgaaaac tggtggtggt
    24961 ttcatgatta gaaatccaca ggggaatttg gtttttagta cgtgggataa tggcaataac
    25021 aaaccgtttc tatcttttgg tgcgcccaat ttcaggtata gcaatgctag ttatataact
    25081 gatggcgacg gtagttcttt aagcattaat ggtagtgcgg gtagttcatg ggagtttaaa
    25141 gtagctggca ggactatgaa atttactagt gatggtatgc taacgttgcc aggttgtttt
    25201 tttggttcat gggaagatgg gaaaattgca aggtttgaac aatcaacggt acaagtatat
    25261 aaaaatttta ctgttagagg tactaaaaac tcaaccgtac cgacagaaca ttatggacaa
    25321 cgactattga acgcttatga aactccagaa tattatttcg ctgattatgg agaagccgtt
    25381 acaggtgatg atggtaaagt tcgtgttgat attgatccca tgtttgctga gacagtaaat
    25441 ctaagtcggt atatgacaca tgtgacacct acagaactag ttttgtgtgc tgttactcat
    25501 gaagatgttg accatttcat cattgaaact agtaagccaa acgtattagt tagatggaat
    25561 ttagtggcac accgtctagg gtatgaagat attagattaa aagaggatac agcatatgat
    25621 agcacagtgc ttgaccaaaa acgtttttaa aacgaagaca aggaggtata taaatggcta
    25681 gcagtttata taatttggct ttagatttca gcaaagaatt aaactacacc aaagctatta
    25741 tggctcgtca aggtgataaa gggattacgg tgacggttaa accgtttcta aatggcttgc
    25801 agatggatac gagtggcgga acatttactt taaaaggaac aacaccatct aaccgttacg
    25861 tagataatgt tgcaactagt gtaactagtg aagaagtcac gttttctctt gatggcacat
    25921 ttatgagtga agcaggatat tataaacact gctacgtaga atatagaaaa gacaatcaaa
    25981 ttttaacaac gcaagatatc atttttttct cactaggagt gtctgacatt tcgcaaggcc
    26041 aagccgatga atatgtttcg caattagaag agttgattcg aaagtacaac gaaacttttg
    26101 atgcttttct ggctgaaatt aaaggtagaa tggatagctt aaataaacag attactgatt
    26161 taactggtca agctaaaaca ctacaagaca agttagatgc tctgaaagaa gaaatttcta
    26221 aattaggtaa cttacatgtg atgtactcca acagcatcga tttcggggaa tatgattatt
    26281 cggggaatcc gaaccttagc gctaaactta atgcttcaag tttttcttta ggtactggcg
    26341 caacagtcgc ggatgataat gatgagattg tgtttacctt agatggcaca aatcaactat
    26401 cgaagtacgc tagcaaagcg caagtgccgg tagcagaagg gaaacaatat acgattagtt
    26461 gtgaaattat gttagaagat gggttcactg gcgacccttc aggaatacgt ctgcaacacg
    26521 catatttgcc aggcggggtt gttgttttac agacagagac agtacctaaa aatgaattga
    26581 atacgtggca gacgcttatc ggaacgcgaa cggtaaaata cacctctgac gtaccaaatg
    26641 aatggtaccc tttgtttaga gacatccgaa cacttaaacc gaccggtaaa gttagactaa
    26701 gaaaaattaa gattgaagaa ggctcaacag ccaccccata tcagccaaac t
//
LOCUS       DACCXM010000028        25721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCXM010000028 DACCXM010000000
VERSION     DACCXM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.030"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            42..674
                     /locus_tag="IVY61_000999"
     CDS             42..674
                     /locus_tag="IVY61_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970128.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(808..933)
                     /locus_tag="IVY61_001000"
     CDS             complement(808..933)
                     /locus_tag="IVY61_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7970129.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1067..1960)
                     /locus_tag="IVY61_001001"
     CDS             complement(1067..1960)
                     /locus_tag="IVY61_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP7970130.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2142..3068)
                     /locus_tag="IVY61_001002"
     CDS             complement(2142..3068)
                     /locus_tag="IVY61_001002"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP7970131.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3153..3914)
                     /gene="pflA"
                     /locus_tag="IVY61_001003"
     CDS             complement(3153..3914)
                     /gene="pflA"
                     /locus_tag="IVY61_001003"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP7970132.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4158..6389)
                     /gene="pflB"
                     /locus_tag="IVY61_001004"
     CDS             complement(4158..6389)
                     /gene="pflB"
                     /locus_tag="IVY61_001004"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP7970133.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6666..9116)
                     /gene="parC"
                     /locus_tag="IVY61_001005"
     CDS             complement(6666..9116)
                     /gene="parC"
                     /locus_tag="IVY61_001005"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP7970134.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9128..11173)
                     /gene="parE"
                     /locus_tag="IVY61_001006"
     CDS             complement(9128..11173)
                     /gene="parE"
                     /locus_tag="IVY61_001006"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7970135.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11339..11773)
                     /locus_tag="IVY61_001007"
     CDS             complement(11339..11773)
                     /locus_tag="IVY61_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP7970136.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11900..12538
                     /gene="plsY"
                     /locus_tag="IVY61_001008"
     CDS             11900..12538
                     /gene="plsY"
                     /locus_tag="IVY61_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP7970137.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12670..13545)
                     /locus_tag="IVY61_001009"
     CDS             complement(12670..13545)
                     /locus_tag="IVY61_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7970138.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13627..14418)
                     /gene="codY"
                     /locus_tag="IVY61_001010"
     CDS             complement(13627..14418)
                     /gene="codY"
                     /locus_tag="IVY61_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP7970139.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14436..15836)
                     /gene="hslU"
                     /locus_tag="IVY61_001011"
     CDS             complement(14436..15836)
                     /gene="hslU"
                     /locus_tag="IVY61_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP7970140.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15850..16398)
                     /gene="hslV"
                     /locus_tag="IVY61_001012"
     CDS             complement(15850..16398)
                     /gene="hslV"
                     /locus_tag="IVY61_001012"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP7970141.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16435..17340)
                     /gene="xerC"
                     /locus_tag="IVY61_001013"
     CDS             complement(16435..17340)
                     /gene="xerC"
                     /locus_tag="IVY61_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP7970142.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17538..18914)
                     /gene="trmFO"
                     /locus_tag="IVY61_001014"
     CDS             complement(17538..18914)
                     /gene="trmFO"
                     /locus_tag="IVY61_001014"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP7970143.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18883..20961)
                     /gene="topA"
                     /locus_tag="IVY61_001015"
     CDS             complement(18883..20961)
                     /gene="topA"
                     /locus_tag="IVY61_001015"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP7970144.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21083..21940)
                     /gene="dprA"
                     /locus_tag="IVY61_001016"
     CDS             complement(21083..21940)
                     /gene="dprA"
                     /locus_tag="IVY61_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP7970145.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21996..22763)
                     /locus_tag="IVY61_001017"
     CDS             complement(21996..22763)
                     /locus_tag="IVY61_001017"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7970146.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22756..23616)
                     /gene="ylqF"
                     /locus_tag="IVY61_001018"
     CDS             complement(22756..23616)
                     /gene="ylqF"
                     /locus_tag="IVY61_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7970147.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23876..24274
                     /locus_tag="IVY61_001019"
     CDS             23876..24274
                     /locus_tag="IVY61_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7970148.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24511..25053
                     /locus_tag="IVY61_001020"
     CDS             24511..25053
                     /locus_tag="IVY61_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7970149.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25136..25687)
                     /gene="lepB"
                     /locus_tag="IVY61_001021"
     CDS             complement(25136..25687)
                     /gene="lepB"
                     /locus_tag="IVY61_001021"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7970150.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
BASE COUNT         6781 a         5451 c         4421 g         9068 t
ORIGIN      
        1 tattatactt tatatactta tcaatttaat ttggagatat tatggaaatt caacgtagaa
       61 ccactaattt tttacattta ggctcttttt ttgcaacttt gacgatctta ttttttctat
      121 gttattcata ccttaagacc tcacatgcgc cttctagtgc attaggctgg ttgcttttgg
      181 atcattctga tatcacagaa gccgaagaaa atccgttttt tttgattttg tcttctcttt
      241 gcggcttact gttttctgtt tatttcggtg cctatttttt tcatcgacat cctggtacgt
      301 taaacgaagc ttcaagcgta ttgattgcat ttaaaacgtt ttctttttta gcaattcttt
      361 tttattcgtt taatcacaat acgcttttct tctttatcat gaatggttta gtcgtgattt
      421 tatccatttt tacttatttt attttttcac tcatttttag ccaattgaaa tgtccgctct
      481 tctttcgatt agttcctgtc acctgtttta tttacagctt gttttccttt tttgtccttg
      541 ctttaatgcc actgaccaac ccttcgacga tccaagtcgc aggaaatctg ttggatatgc
      601 tcttcatcgt cagtctaggt gtatttatgt ggcaaagaaa aaacaaacaa aataaaaagc
      661 attttgaagc ctgactatta cgacttcata aatgcttttt tagttttaaa cacttcaatc
      721 taaaagaata acccaagatt ggataaatta ttaatgactc gccattcgtt tcttagtgat
      781 tctggattat caaatggact tcctaatcta atcagctagt tcttcccata aaatgccata
      841 cttgactgaa tcataatatt gattatttaa aaatctaact tttcttatca ctacttcctt
      901 tgtcattcca catttctcac catttttttg cattcctttg tttccagacc atgtagtgaa
      961 tctaataggt ggtagatagg caaaatactc aaataattct tttaaccata aaggaagaga
     1021 aaggctcctc ctgcaagagt cagtataatt tttgcttact gctctcttag gttttactca
     1081 ctaaaactgt tccatattct ttgatataag aaaatgcatg cggtataaat ttcttgctca
     1141 gctcagaaaa gatttgtcgt ttaagaaagg ccatttcctc cattgtcaat gaaggatcca
     1201 atgttggtaa ttcaatctca aaattgtttt tttcgacaat aggtaattct aatgtgaatg
     1261 attcttcttc ctttatagta ttcgtcacaa gtagagactg atggaaaata ctaggccggc
     1321 gatttttata tttattctta cgtatcctgt caacgataac ttctatttga tcaaaaaagt
     1381 acgattcatt tacgtctcca ataagttcgg ctaattcttt gaatgccttc gttacaggaa
     1441 gctgattctt ctgtacaaaa aagtaattag ccaaagtaag cgggaagtgt tctatagagg
     1501 aaagtgtgat aaaacggtac ttctcatttc ctgcagttgt cctcagcaat tctgtctctc
     1561 ggctgtttct tactccataa aaatacgctt ctttccgttc atcttcgaaa caatacgccc
     1621 aaataacttc tcccatcgct aatttttgtt catcaacaga gagtctcttc aattgttcag
     1681 ctatggtttc agcagccgaa gtggcttgtt gcaagtaatc tttaggatca aatattggaa
     1741 agttcaaatg atatcttcgt tcttccctct tgattaatcc tgcatcgatc aataaatcaa
     1801 gaaaatggga aaatttattt tgcgaaaatt cttttttcaa ttctcttaga atcgtttctt
     1861 gttctcgatg ttcatataga taatcggtca atgaagaaaa aagcggatca tcggtcagtt
     1921 tttgataaaa gttttcctta ttttttgaat aatagttcat atatgttctc ttttctatcc
     1981 gttttatttt acttaaaagg cttttacgta aaaaggagga agaacaagag tcataattga
     2041 ctgttattct cctccatttc ttcggccttt agtatgttct atgtttcttt ttatatcaat
     2101 cgcttatcca ttcattttga atgcttcata ctaccatttg attagctaaa tacagccgtc
     2161 aattgaggta cgacttgttt tttacgtgaa acgacacctt taagcaaagc acggttgttg
     2221 gtcaaagttg tttggaaagc ttcttctact ttttcttttc ctttaccagc aacaagtaat
     2281 tctgaatcac tgtctaagat gtttgtgaca actaatacga acagatcgta gccatttgct
     2341 ttcatttcat cttccattgc tgcttctagc tctgcttgtc gatccatcac ttcattaagg
     2401 tccactgtat tgacttgagc gatacgaacg ttggaactgc ccattgggaa actttttgca
     2461 tccaaatcaa gaagaaccgc agctgattta tcacttagat ttgttcctgc tttcaacatt
     2521 tcaagtccat aagtgtttag atcgacttca gcgattgctg caagttcttt tgcagctgct
     2581 tcatcttcag gcgtacatgt tggtgatttg aacaacaatg tatctgaaat gatagctgag
     2641 agcatgattc ctgcgatttc tttaggaatt gttacttggt tttctttata tagttttaaa
     2701 ataatcgtac ttgtacatcc aactggttct gcacgatagt acaatggatt agctgtttca
     2761 aagtttgcaa tacgatggtg gtctactaca gctaaaatat ttacttcgct gatatctgaa
     2821 atactttgtt ggaattcatt atgatccaca agcatgacat ctgcattttc tgctttagct
     2881 gactctacta ctcgtggtgc agatagctga aagtggtcta atgcgtattg tgtttcttca
     2941 ttaggtgtac ctagagcaac tgcttctgtt tctttaccta attcattttg caagtgtgaa
     3001 aacgcaatgg ctgccccgat tgcatctgta tctggatttt ggtgaccaaa aactaaaatt
     3061 tttgacatgt cattcgctcc tttgtgattt ctcctattta ataatagcaa aaaaacggaa
     3121 agaagcaata cttatcttct ttccgatgcg acctatcgcg ttaaatattt tgtatactct
     3181 tctgtatgaa gcaatcgttt agcattttcg acacgttctt ttgtaggtgg ttcgatccct
     3241 tctaatggat atttcaaacc aagtgtttcc cacttgtatt ttcccatcgt atgataagga
     3301 agaatctcta ctctgtctac atttgacaag gtttgaataa agttgtctag tcggatcaaa
     3361 tattcatcat aatcacttcg ctcaggaact agtacatggc gaatccaaac cggtttgttg
     3421 atttttgaca aatattgagc catttctaaa atattttcat tgtcccaatg cgttagtttt
     3481 ttatgctctt cattatcgat gtgtttgatg tcaaataaca gtaaatctgt atacttcatc
     3541 aattcttgaa aacgtgaaaa gaaaggttct tcatacgtaa aaggttttcc gcatgtatcc
     3601 aatgtagtat gcatcttccg ctcttttgct tttttaaata gttcgatcaa aaaatcgatc
     3661 tgcaatagag gttctccacc actaacagta ataccacctt tatctcccca gtaaggtctg
     3721 aagcgttctg ctttatctaa aagttcatct gctgtgtatt ctttccctcc gccaatattc
     3781 caagtatctg gattgtgaca aaattggcaa cgcatgcggc atccttgcat aaacacgaca
     3841 aagcgcagtc caggtccgtc tactgaaccg aaagtttcga tagaatgaac atagccgatt
     3901 gttttttctt ccatttaagt tcacatcttt tctttgattt cattttaagt acaagttagc
     3961 tgtcgaagcg aagctttctg aacaaaattt ttcgcaattg ccgcttcgtt atgtgtaaaa
     4021 tttacaggga cggaacatac aagtcctgcc cctgtaaatc gtttctttca aataagcata
     4081 ctgattattc gattttgatg cgatggctat tcaacgtcct ttcgacgcta agtcgaaaaa
     4141 cttttttcac gaacttttta cattctgtcg tgtgaagttc tcatcaagac atctaattgt
     4201 tgttctcttg tcaagtcacg gaatttaact gcataaccag atacccggat cgtcaagttc
     4261 ggatatttca ttggattttc cattgcatca cgcaataaat cattactgaa tacattgaca
     4321 ttcaaatgat aagctccttt tgagaagtat ccatctaaga cattacgcaa gttttcgatt
     4381 cttgtatctt gttcttttcc aagacctgaa ggattgattg tttgggtatt tgaaattcca
     4441 tccaatgcac agccgtaatt cattttagct gttgagttca ggctagctaa taacccactt
     4501 tgttccgcat tgtaacttgg atttgctcct ggcgcaagtg gttttccagc ttcacggcca
     4561 tcaggagtat ttcctgtatt tttaccgtaa acaacatttg aagtaatcgt caataatgaa
     4621 gttgttggtt ttgagttgcg gtaagtttta tgacgtttga tttgtcccat gaagtattcc
     4681 aagatccatg aaactaattc atctgcacgg tcatcatcat ttccgtattt agggaagtcg
     4741 ccatccactt caaaatcaac tgctattcct ttttcattac ggattgcttt tacttttgca
     4801 tatttgatcg ctgacaagct gtctgccgca tgagagatcc cagcaatacc tgtagcaaat
     4861 gtgcgttgca agtcagaatc catcaaagcc agctgagctg cttcataaga atatttatca
     4921 tgcatataat gaatgatgtt caacgtattg acatatagtt ctgctaacca atccatcata
     4981 ttcatgaagt tatgtttcac ttcttcaaag tctagtattt ctgatgtgat tggacgatat
     5041 tctggtccaa cttgggcgcc agtcacttca tcgactccgc cattgatggc atataatagt
     5101 gttttagcca agttagcacg agcgccaaag aattgcatgt cttttccgac aactgttcct
     5161 gatacacagc aagcaatcgc acaatcatca gaaccccatt gttcacgaag ctgattatca
     5221 ttttcgaatt gaatcgaaga actttcgata gcgattctgc tagcatatgt tctgaatcct
     5281 tctggaaggt gttcagaata caaaacagtc aagtttggtt ctggtgaagg tcccatattt
     5341 gttaatgtat gaagcagacg gaaatcattc tttgtaacta atgaacgacc atctaatccc
     5401 atacctgcaa tggataaagt agcccagata gggaatcctg agaataattg gttatattca
     5461 ggtgtacgcg cgaatttgac catacgtaat ttcattacta agtggtcgat caattcttgt
     5521 gcttcttttt ctgtgattac tccattatcc aaatcacgtt ggatatagat atcaaggaat
     5581 gctgagatac gtccgattga catcgcagca ccattttgtg atttgattgc tgctaaatat
     5641 ccgaagtata gccattggac agcttctttt gcatttgcag ctggtttaga aatatcatag
     5701 ccatatttcg cagccatttc tttcatttgc gctaatgcac gatattgttc agtgatttct
     5761 tcacgtaaac gaatcacttc ttcactcatt gtatgataac cagtcatatc atgatctttt
     5821 ttcttttgtt ccatcaaata gtcgattcca tacaatgcca cacgacgata gtcaccgatg
     5881 atacgtccgc gaccgtaagc gtctggaagc cctgtaatga ttttgttttt acgagcagca
     5941 cgcatttcag cagtataagc gtcaaataca ccttgattat gtgttttgcg ccagtcagtg
     6001 aaaatatgtg tcaattcatc gtttggtgta tagccattgc tttttagcgc attgttagcc
     6061 atattgattc cgccgaacgg catgaatgct tgtttcaaag gtttgtctgt ctgtagaccg
     6121 acgatttttt ccaaatcttt atccaaatag cctgcatcgt gtgaagtaat agttgatacg
     6181 atttctgtgt ccatatcata gacaccgtta cgctcgaatt ggatatcatt taacttttgc
     6241 aattcatccc ataatttttc tgttgcttct gttgggcctt ccaaaaagct gtcatcaccg
     6301 cgatattcag tgtagttatt ttgaataaag tctctagtgt ctacgccttc tttccatttg
     6361 gtacctttaa atccattcca ttgctccatt gtatggacct cctaaatttc tgttttatgt
     6421 aacagttttt acgtgattag tataacacag caccctatct ttgcaagggt tttcttgttt
     6481 tttatttaac aatttttatg ttttatttac atatatttaa atcaacgttg acataccaac
     6541 atttaagcat tgtgaacact ttcatctttt tttgcaaacg tataaggatt tattaaaaat
     6601 gaatgaaaaa gagacgccac ctgtattaat acagatggcg tctcttcatt aaaatactac
     6661 aacagttacg actcgatgaa aatctcagga gcatcgataa cttcgatgac ttctccacct
     6721 tttttctcat ccatcgcaaa tgatccattc gatgttcgat cacctatagg gaaatcagtt
     6781 gggtcaatcg ttaactgctg ccctttttga ttcatcaagg ttatttcttt tgatcttcct
     6841 tccgacatat agataatacg gtgcggattt tttttcagct cacgcatgac cgtcactccc
     6901 cgcttcgcac gtgaagtttg gctgatttct tgagcaagca tgcgtttaac agctcctcgc
     6961 tgagtaacga ttacgatcgg cgtatcacct tctgctaaca caagcagccc gttgataatc
     7021 ctatctccat ctttcaaatt catagacttc actccagctg cctttgctcc aacaacggga
     7081 acatctgtta aaggatagcg caaacccatt ccttgatgcg ttactaagaa aacatctaag
     7141 cctggctgat cttctttcag atacacagca atcagttcat cttcttccgc ctttagcttc
     7201 atacagctga gcggacggct cttataggtg cgccatggtt cgaactctgt cattcgtgtt
     7261 tgcttgatca atccattctt gctgatgaat acaaatgttt tctctgcttg aattttttga
     7321 tatgggaaaa cagctaaaat cgattcgtct acagacagat tggagattgt ttgagaaatg
     7381 tgctcaccag catctttcca tttcaaatcg ggtagttcat ggacagggcg gtagatcaca
     7441 tttgctttat ttgtgatcag aagaacatgg tccaatgtac tcaattcacc tgagtatagc
     7501 aagtagtcgc cttctcgcat cccgatttct tctggtttgg aagcactata agaacgaagg
     7561 cttgttcgct tcacatagcc ttctcgcgtc acactgacaa ttacgtcttc ttgtgccact
     7621 aagacttgcg tatcaatttt gatttcttcg atttcgtctt cgattgtcgt tagacgagcg
     7681 gaagaataat tttttttcac ttctcgcaat tcttttttca taacagagaa aagctctttg
     7741 tcatttgaaa ggattttatt caactctgtg atttgcgcaa tcaagctttc ggattccttt
     7801 tgaagctctg tgatatccgt gttcgtcaaa cggtataatt gcaatgtgac aattgcttct
     7861 gcttgttctt ctgtaaattg aaagacgtcg actaaattct ttttggcgtc ttttttgtct
     7921 ttactttcac gaatcgttgc gattacttca tccagaatcg ataaagcttt catcaatcct
     7981 tctacgatat gctgtctttt ttgagctttg gctaaatcga attgactacg ttttgttatg
     8041 acttgctttc gatgctcgat ataacttctt aaaatgtctt tcagtccaac ttgatgcgga
     8101 gtcatatggt cgatagcgac catattgaaa ttgtaattga tctgtagctc agtgtttttg
     8161 aacaaataat tcaaaattcc ttgagcattg gcgtcttttt tcaattcgac gacaatttgc
     8221 agacctgttc tatcactttc atctcgtact tcagctattc catcgatttt cttattcaaa
     8281 cggatctcat ccattttttt caccaaggtc gctttattta cttcatatgg gatttctgtg
     8341 atcacgattt gctgtttatt acccttcatt gcctctatct ttgttttcga acgaagaata
     8401 acttttcctc gtcctgtttc ataagctttt ttgatttcat cttttccttg caagatccct
     8461 cctgttggaa agtcaggtcc aggaatatat tccatcaatt tttcaaggct cgcttgtgga
     8521 tgatcgatca tatagattgt gccatcgatc acttcagcta agttatgagt cgggatttct
     8581 gttgcatatc cagcagatat acctgtcgat ccattgacga gaaggtttgg gtatcgagct
     8641 ggaaggactg ttggttcttt ttctgtatca tcaaaattcc acacgagatc gactgtttct
     8701 ttctcgatat ctgctagcat ctctccactt aacttggata atctggcttc agtataacgc
     8761 atcgcagcag gtggatcacc gtccatactc ccgttattcc cgtgcatttc gatcagtact
     8821 tcccgcaact tccagtcttg gctcatgcgg accatcgctt cataaatact gatgtcacca
     8881 tgaggatgat aattccccat gatatttccg acagattttg ctgatttacg gaatcctttg
     8941 tcatacgtat tgccatcttt gttcatggca aataatatcc ggcgttgcac tggcttcaat
     9001 ccatcacgga tatcaggcag tgcacgttct tggataatat atttggaata acgtccgaag
     9061 cggtcaccca tcacttcttc taaagttaat tcttgtactt cttgtcgatt ttccatcagg
     9121 tgacctccta ttctacttca aataactgat tgttttcatt tttatctgct cgttcttcat
     9181 ctaataaatc attagaaact gaaggagaga tttcagtatc ctctttttta tctaagatac
     9241 tgccatcttc ttcgagcgta aattgcacgt gattttcgat ccattttctt cgaggttcta
     9301 ctttgtcacc catcagtgtg gttactcgac gttcagcttg cgcagcatca tcgatacgga
     9361 cgcggatcaa cgtacgggaa gtcggatcca tcgttgtttc ccacaattgt tccgcattca
     9421 tctctccaag tcctttatat cgctgcagca tatatccttt gccgactttt ttaatcattg
     9481 ctgccaattc gtcatctgtc cacgcatatt caatgacttg cttctttcct tgtcctttgg
     9541 atactttata taaaggcggt aacgcgatat atactttccc agcttcaatc agcggtttca
     9601 tataacgata aaagaaagta agcagtaata cttgaatatg ggcgccgtct gtatccgcat
     9661 cggtcatgat gattactttg tcgtaattac agtcttcgat agaaaactca ggaccgactc
     9721 ctgcaccaat cgtatagatc atcgtattga tttcttcatt tttcaagata tcttgcattt
     9781 tagctttttc ggtattgatg acttttcctc gtaaaggtag aattgcttgg aactttctgt
     9841 ctcttccttg tttagcagat ccgccagcag aatccccttc gactaaatac aattcatttt
     9901 tcttagggtt tcgggattgt gcgggtgtca attttcctga aagaagagat tcgccttttt
     9961 tacgcttctt cccatttcgg ctttcttctc tggctttacg tgctgcttct ctcgcttctc
    10021 tggctttgat tgctttacgg accaacatct ggctcatttc gctattttcc tgcaaataaa
    10081 agcccatttg ttcactgatg acgttatcta ctacggtacg agcaactggc gtaccaagtt
    10141 tttcttttgt ttgcccttca aattgcagaa gattttcagg tacacggata gaaagtacag
    10201 cagctaaccc ttctctgaaa tcactacctt ccagattctt gtcccgttct ttcagcaaac
    10261 caacttttct cgcatattca ttataagatt tcgtcatcgc cgctttcata ccagcttcat
    10321 gggttccccc atcttttgtc cgaacgttat tgacaaaaga taaaacattt tctgaataac
    10381 cgtcattgta ttgataagcg acctctactt cgataccttc tttttctcct gaaaaataaa
    10441 caactggtgt caatgtatct ttttcttcat tcagataatc aacaaattct ttgattcctt
    10501 cttcgtaatg aaaaatttcc ttgactggtt cttctccacg aagatcagaa agagagattt
    10561 taacgccttt tagaagaaag gcagattctc ttaaccgttc ggctaaaatt tcatatgaaa
    10621 atttcgttgt actgaatatt gtatcatctg gtaaaaaatg gacagtcgtc ccgtttgctt
    10681 tttttgtttt tcctatcttt ttcaaagtac cgtcaggttt accgccgttt ttaaatttct
    10741 gctgatactc tacgccatct ctaacgatcg ttacaattag ccatttcgat aaggcattta
    10801 ccacactggc tcctacaccg tgcagtccac cagaagtttt gtaccctcct tgaccaaact
    10861 ttccgccagc atgcagcaca gtaaaaatca cttcaactgt tggtattccg gaagcatgca
    10921 ttcctacagg catcccacgt ccgctatctg caacagttat actattgtct tcatggatgg
    10981 tcacatcaat ctcactgcca taacctgata atgcttcgtc aactgcatta tcgacgattt
    11041 catagactaa atgatgcaag cctcggctgt cagtggatcc aatgtacatc cctggtcttt
    11101 ttctaactgc ctctaatcct tccaatactt ggatggacga gtcattgtat tcgttgttta
    11161 ctttttttgc caaggcaaac gctccttcaa taatactttg cggcaaaacc gcaccacgat
    11221 tatcatacta gaaaaggtcg tgaaaaaaaa gagggtagac gagaaatcaa ttttaattat
    11281 ctcctaaccg ctttttcgac attcccctct atcgatcatc tcttctttgt ggtcattttt
    11341 atttttggtt ttctttcaac ttaccaagtt caatcttaat acaacgatcc attacgatat
    11401 ctgtacgtcc tgcttttctt agcatctcag ctgcttcttt gttttcaatc cctaattgtg
    11461 cccagaacac tttagcatca gttttcaaaa aatcacctgc tatttcaggt aaaaattcac
    11521 tccgtctaaa tacatctacg atatcgattg gatcatcgat ttctgtcaaa ttcgcataga
    11581 ctttctcgcc tagaatttct tcaccagcca ataatggatt tactggtatg attttatatc
    11641 cattgtcttg caatacctgt gcaaccatgt aacttgttcg ttctggttta ttgctcaagc
    11701 ctacgacggc aatatttttt gcttcttcca aatagttgaa aatcgtttct tgtgttggat
    11761 tttttacaga catttctgcc accccttgct tttcttttta ttccatctat tatacttgta
    11821 tctttcattt tttcaattgt tcctgttacc tattcatgct aaaataaagg atagttttca
    11881 acttgaaaga gggtaaaaaa tgaaattgat cttattactt attttagcat atcttttagg
    11941 ttctattcct tccggtgtct ggattggcaa attgtttttc aaaaaagata tccgacaaca
    12001 cgggagcggt aatactggaa cgaccaatac atttcgtgta ttaggcaaaa cagccgggat
    12061 tgttgtcctg cttatggata ttttaaaagg tacattggct acttgtttgc cgatgatctt
    12121 ccacctttct gggattaacc ctctttggtt tggggtatgc gccattcttg gccatacatt
    12181 tccgattttt gctgggttca aaggaggaaa agctgttgcg acaagtgcag gaatgctttt
    12241 aggatttaat cctgtatttt ttgtttattc ttgtatcatc tttatcgtta gtttattttg
    12301 tacaagtatg gtgagtttga gcagtatgat cagtgcagta ttgatcactt tatctacgat
    12361 cgtccttcct tatgtagctc ctgtgatttt ggctaagccc aattggttac tgacgatcat
    12421 tgccttttta gtttcttcct ttatctttta tcggcataaa gacaatatca agcgtatcag
    12481 aaaccatact gaaagtcgaa tcccgtttgg tttgaatagc agcaagaaaa aatcctgata
    12541 ctttttcatt ccaaagagat aaaacgggag tgtgacaaat cgatgtcaca ctcccttatt
    12601 acttacagga tagctttttt acacaccttg gtaaacggtg ttccctgcta acttctcggt
    12661 cacaacctct tacttcactg tgatggaata ttttgtcttg aagatttcat gggcacctag
    12721 ttgattgatc cctacttttt ccatcaagtt tccactactg ttcaccgtgt cagcgattcc
    12781 gcaccacggt tcgatacaaa cgaaaggcgc ttcttttgga taaggagacc agatccctac
    12841 ataaggcata tctttatagc ttaaagttac gctatgggaa gatttttcac ttcgaatggc
    12901 gtacgcattt agaccccttg tttcaaaaac caaggcatct tgctcaaaaa gttcacgatt
    12961 caaagccaga ttcgtatttg tctgacctaa cgtcttttga tccatatcga tgaatgggcc
    13021 tttcaatggt aggcgtaatc ttgatttcaa tggagaaaat gccagataga aatcttcaaa
    13081 tgacaatcct tcttcaagag gtacattgaa agcaggatgt ccaccgatag aaaagaacat
    13141 ttctttttca ccggtatttg ccacttgata atgaaccgtg atgccgtctc ctcctaattc
    13201 atagctgatc actaattcaa aatcaaatgg atacttttct aatgtctccg gcgtactcct
    13261 taagatgaaa ctagccattt cttgtccatg ttcaatgact tcaaaatcca tatcccgagc
    13321 aaatccgtgt tgccccattt gatatgtttt cccttgataa acatactgat catcttttaa
    13381 acgtccaaca aaagggaata aaaccggtgc atgtctgccc caatacgctg gatcaccttg
    13441 ccagatatac tctaaaccat gttcttttga tttcagactg atcaattccg caccgtgttc
    13501 agcgatttcg gcgatcagct ggtcattttg aataataaca gtcattgcca ttcctccaac
    13561 ttcgacttac tttttaaaat gggaaggctg gaacaaacat ttctatctgc cccagtcccc
    13621 ttacttctaa taactgtgtt ttgctaactc gtctttaaaa cgggaattca atacttttaa
    13681 ataagttccc ttcatgccta acgaacgaga ttcaatgatt ccagctgatt ccaattttcg
    13741 taaagcattg acgatcacag aacgagtgat gccgatacta tcggcaatat tagaagcagt
    13801 cagacgacct tcatctccat ccaatgcttc aaagatagct tgtacagcct tcaattcgct
    13861 ataagataat gtgttgattg ccatttggac agccgttgca ctccgtacat ctgcttcgat
    13921 gcttcgagac ttctgataaa ggatctgcat accgacaacc gtagctccat attctgctaa
    13981 gactagatcg tcatctgcaa acgattgttc catacgagat aagataatcg tacccaaacg
    14041 ttcgccagct ccaaagatcg gaacgacagt cgtcagacca aatggatatt tctctcttga
    14101 ctctaccgga aatgcagtaa tatcactagt aattgggata ttagcttcag ttttcatcaa
    14161 ctgtgccact gtatcagtat aagattcagg aaattgtttc tcaacaaaca ttgttttgat
    14221 tcgttcattg ttcacatcgt gacgttcatt ataacctagt aatactccgt cacttcctat
    14281 gatataggtg ttgctgtcaa gaataccacc tagtgtgaca gccattttat cgtaaggcaa
    14341 atctgcttgc atgtcaaaag tattcttttg ctgcaaaagt gtgtttatct tgcgggtttt
    14401 cgttagcaag tctgtcattg tcgtcctccg attttttata aaatatagcg acttaaatct
    14461 tcatttgccg caattgagcc aagtttgtca tccacatatg cttctgtgat cgttatttct
    14521 cccatttgca tatctggtgc ttcaaataat agatcttcta gtagtttttc caaaatggta
    14581 tgcagtctgc gtgcgccgat attatctgtg tcacggttga catcgaaagc gatttgagcg
    14641 atccgttcaa ttgcttcttt tgtaaacgta actgacacat tttctgtacc gatcaacgcg
    14701 acgtattgtt ttactaaagc attatttggt tcggtcagaa tcttcacaaa atcttcagct
    14761 gtcaaatcat ctaattctac ccgtatcggg aagcgtcctt gaagttctgg aattagatca
    14821 cttggtttag acaaatggaa agctcctgaa gcaatgaata ggacgtgatc tgtttggatg
    14881 atcccgtatt ttgtgtttac ttgtgaacct tcgacaattg gcaatatgtc tctttggacg
    14941 ccttctctgg atacttctcc agattgctgt gatttagaag tgatcttatc gatttcatcg
    15001 ataaaaataa ttccactttc ttccgctaat ttgattgcct cactatggat atcagcatcc
    15061 ttgactaatt ttgctgattc ttccttgatc aataactcta aagcttcttt gactgttacc
    15121 gtccgttcca cttttttctt cggtgtcaaa gcacctagag tttcatttag gtcgatcccc
    15181 atttgctcca atccattgtt catcattggt gcaggtttct tttggtcttc aacttcaatg
    15241 gttacttcac gactatccag taaacccttt tgtagttgtt ccaagactgt acggcgattg
    15301 attcgaatat cttctgtcac ttcttcttgc ggttcagcgg tattttgctg actagcatta
    15361 aaaagattca tcatttgttc aaattgatta gtggattgtt tctgttcttt tttgatacca
    15421 ggtaccaaga tttttaccaa acgtttttct gcattttttt tagcacttgt gtatacacgg
    15481 ctgtactgct ctttttctac gatttggatc gcattttcta ctaaatcccg aaccattgat
    15541 tctacatctc ttccgacata accgacttca gtaaattttg ttgcttcgac tttgacaaat
    15601 ggtgcattga cgatttttgc caaacgtcgt gcgatttctg ttttaccgac ccctgtaggt
    15661 ccgatcatca gcatattttt tggtgtaata tcttgttgca ttttttcatc taactgcaaa
    15721 cggcgatagc ggttacgcaa agcaacggca acagattttt ttgctgcgtg ctgtcctacg
    15781 atatattcat ctaattcact tacgatttgt cttggtgttt gttttaattc atacatatct
    15841 actcacctct tacagttctt ctacgataat attatgatta gtaaagatac agatatctgc
    15901 ggcaatattt aacgcatttt ctgcaatttc tttcgcactc atgtcgtttt gagcatagtt
    15961 tttcattgct cgggctgcag ccaacgcaaa attccctcct gatccgatag ctaagatacc
    16021 atcatctggt gtgataactt ctcctgtacc tgaaacaaga agcatttctt tatcattcat
    16081 tacgatcagc atcgcttcaa gtttttgcat agattgctgt gtccgccatt cttgagctaa
    16141 ttcgacagct gcgcgttgta gattcccgtt atattcgttt agtttgcctt caaatttttc
    16201 ttctaaagta aacgcatctg caacacttcc ggcgaaacct acaacaactt ctccattgta
    16261 gatacgtcgt actttctttg cagtgccttt catgacaaca gattcaccca ttgttacttg
    16321 gccatctcca gccatagcaa atttcccgtc tttttcgacg gcacagatag tggttgaatg
    16381 aaatggtgat tcgaccatgt ttgtttcctc ctaaatttgt tcttttcctt atttttacgc
    16441 tcttggatgg aaagaacgat agtttttttg taaactttct tttgttacat gggcatagat
    16501 ctgggtagtc gataaattgg catgtcctag caattcttgg actgtccgca aatctgcccc
    16561 attatttaat aaatgagtag caaatgtatg gcgcagcatg tgaggatgga tatcactatc
    16621 caaactgctt tttttgatca tttgattcaa gacatattca attccagtag gcgtgattgg
    16681 ttttccatga tgattcacaa aaacgatatc atgctcttcg tgatattttg tcatcaatac
    16741 ctggcggcct tccctgaaat attcttgcag agcatcttga gcaaaagaac ctagaggagc
    16801 atatctctct ttgttccctt taccatggat caacatgacc tctgaatcaa aatcaatgtc
    16861 tgataccgac aaactgctac actcacttaa acggatgcct gacccgtaaa ggacttccag
    16921 taaagcgcgg ttcctcaaat ccaaaggact atctcctgct acgctgtcaa aaagtgcttg
    16981 catttcattt tcataaaaaa aacgtggcag tctcaaattt ttctttttga gatgaacgta
    17041 agaaaatgga ttttctttta tcacttcttc tttgagaaga tactgataaa acgaacgtag
    17101 gctggctatc ttgcgactga tagaatttct gctatagcta ggacgatgat cagctaatgc
    17161 actcaaatag acacgaaccg taaaatattc cgccttcagc agatcagcgt ctccagattc
    17221 ctccaaaaaa tgtttgaaat ggagaatgtc ttcttcatat gcttctttcg ttttttctga
    17281 atatcctctt tcgacaatca aataacgcaa aaatttttgt tcccacgtgg cttctgtcat
    17341 tttctcacct tccttttgtc ccagagaata atttagcaca gcgtttataa aaagacaaat
    17401 aaattgtcag aatttaacca atatttctaa gtttgttcac aattggcgca attcctcaca
    17461 ttttgtttga gaaattctta agaaaaacgc aagaaaaaga gcgagactat ccgtctcacc
    17521 ctcttcatgg agtttcctta ctcatttggg attgcagttt tttctaattc tgtttttatt
    17581 gcctcattag cttttaaagc tcgttgtgca attgcttcat atcttgcttt tttgtctcga
    17641 atgcgctctg gcagttcagg aaacagacca tagttggcat tcatcggttg gaaatgtttt
    17701 ccttccgcat gagtaatgta gtaagccata cttccaatag ctgtttcttg tggaaactcg
    17761 actggttcca tttcttttgc taagcgtgcg gcgttgattc cagcaagtag tccgctcgct
    17821 gcactttcaa catagccttc tacaccagtc atctgacctg caaagaacag atcttcgcgt
    17881 ttcgttgatt ggtaagtcgg tttcagcaat tctggcgaat tcataaagct gtttcggtgc
    17941 atcacaccat agcgaacgaa ttcagcattt tctaacccag gaatcatccg gaaaacacgt
    18001 ttttgctcgc cccatttcaa gtgagtctga aagcccacga tgttgtataa agaagctgca
    18061 gcattgtctt gacgcagctg gataacagca taaggacgtt tgccagtttt aggatcttcc
    18121 aatccaactg gtttcatagg tccgaaaagc atcgttttga atcctcgctg tgccattact
    18181 tcaattggca tacatccttc aaaatatttt tctttctcaa aagatttaag ttctgccact
    18241 tcggcattga ttagtgcctc atggaaagct ttgaattcct cttctgtcat cggacagttc
    18301 aagtaagctg cttctccttt gtcatagcgg gacttcaaat atactttatc catatcgatc
    18361 gtagctttgt ctacgattgg tgcagctgca tcatagaaat aaaaaccatc tgaaccattg
    18421 aatgctttga tgctttctgc taatggatga gaagtcaaag gtcctgttgc tactacggtg
    18481 attccttccg gtatttcagt aatttcttcg tttatcactg ttactaatgg atgctctttg
    18541 acttttcggg taattgtttc agaaaaagaa tcgcggtcaa cagctaaggc accaccagct
    18601 ggcactgctg tttcatctgc cgaagtgatt actaccgaat tcaatcggcg catttcttct
    18661 ttcaataacc ctactgcatt agccaaactg tttcctctta atgaattgga acatactagt
    18721 tcagcaaaat tacttgtgtg atgcgcctca gtcgatttga ctggacgcat ttcatataaa
    18781 cggacaggga ctcctgcttg tgcgatctgc caagcagctt cacttccggc aagccctgct
    18841 ccaacaacat ttaccatttt caatgaaaga acctcttttc tattattttt ggatgtcttc
    18901 ttcgtaatct ccatttacac agactacttg tttaccgcct ttgactttct tctcgaccag
    18961 atatccgtca cattttggac aatttctgcc cactggttta tcccaagaag tgaattcaca
    19021 ttctgggaaa cgggaacaac catagaatag tctgtttttc ttagattttc gttcaatgac
    19081 ttgtccttca tgacatactg ggcaagtcac accgatttct tttacgattg gtttcgtatt
    19141 ccgacaatca ggaaaattac tgcaagcata aaacttccca tatttcccta attttatcac
    19201 catcggatgt ccacagacat cacaatcgaa tcctgctggt tcatctttga tttgaatttt
    19261 ttcgattttt tcttccgcat tcgtcagttc tttttcaaac ggtttataga aacgatcaac
    19321 cactttgacc cacgcttccg tgccttcttc aattttatcc aaatctcctt ccatttctgc
    19381 tgtgaaatgg atatcgacga tttgagggaa gaattcacag attaatgaat tgacaatttc
    19441 tcctagttca gtcggctcaa atcttttttg tgtcaatttt acatagtagc gtctttggat
    19501 cgtatcaagt gttggtgcat aagtggaagg tcgccctacc ccgttttctt ctagtgcacg
    19561 aatcaacgta gcctcactaa aacgtgctgg tggttgagta aaatgttgtt ttggttcaat
    19621 atcaacagat tggactacat cgccttcttc aagttctgga agaatatttt ctttctcttc
    19681 tttgccgtca tctctacctt ccacatacac ttgcatgaat cctttgaatt tgatttttga
    19741 accgttcgca atgaagatga caccattttg ttctaatgtc actttcattg tatctaaaat
    19801 agctggtgtc atctgactag ccacaaaacg tgaccagatc aatgtataaa gtttcaattg
    19861 atctttatct aaatattgtt tgagttcatc tggtgtacga agcacactcg ttggacgcac
    19921 agcttcatgg gcatcttgtg cgccttgagc atttttgatc tttcggtgat catgtctaga
    19981 aaattctttt ccatacgtat cttcaataaa tgctgcggcc tctgcttttg ctgtatcggc
    20041 aatccgggta gaatcagtac gcatgtatgt aatcaagccg actgttcctt gtttacccaa
    20101 agtgattcct tcatataatt gctgtgctac catcatggtt tttcttgttc gaaagtttaa
    20161 cttacgggca gcttcttgtt gcaggctact tgtagtgaaa ggattcgcag ggtttctttt
    20221 gcgctctttc ttttctactt ttttaacttc gaagtctgtt ccatcaattc tagcagtgac
    20281 ttcttttaca ctttgtgcat ctggtaattt ctttttctta ccgtcgattc cccagaaatt
    20341 tgctttgaat ttcttgcgag cttttttaaa attaccgtcg atgctccaat attcttctgg
    20401 aacaaattct cggatctctt tttcacggtc aataataatt tttaaagcga cagattggac
    20461 acgtcctgca cttaaccctt tcttgacctt acgccagaga ataggactga tcgaataacc
    20521 aacgattctg tctaaggtac gacgagcttg ctgtgcatct actaaatcta catcgatcgt
    20581 tcttggttcc ttaaaagctg ctttgactgc gtcttttgtg atttcattga agaccacccg
    20641 atttttttct ttcagatcca acccaagtag gtacgctaaa tgccaagcaa tcgcttctcc
    20701 ttctctatcc gggtcacttg ccaagtaaac tttttgagct ttttttgctg cggctttcag
    20761 gcttttgatg acatcgcctt ttccgcgtat agaaatataa tggggttcat aattgttttc
    20821 aaaatcgata cccattttac ttttcggtaa atctcttata tggccaacac tggccattac
    20881 tttatagttt cgtccaagat atttctcaat tgtcttggct tttgcaggtg attctacaat
    20941 cactaaatat ttatacgcca ttactgaatg gactcctttc cattgtcgtg ttcctactat
    21001 taaaagagga ttcatacaaa tcgtagctaa tcatatccat tcagaaaacg tttgtcaaga
    21061 aaagcaaatg aaaagtatgg tgttattctt taaattctgc caagatatct tcacccgaaa
    21121 ccgtgcaaac tgcaccgtct tggatcaaat gatgacatcc ggtagaatgt tctgttaaaa
    21181 tatttcctgg aacagaaaaa actgttcttc caagatccaa tgcttgctgt gctgttatca
    21241 gagagccgct ttttttcttg gcttcaaaga cacatgttcc tttagacaaa gctgcgataa
    21301 tccgatttct cattggaaaa caatgctttt tcgggccgga ttgaggagga tattcactca
    21361 aaagaagatg atcatttgca atcacctctt gcaagtcacg attttcagca ggatagactt
    21421 gctctatccc tgttccaatg actgcgattg tccgtccacc atgagagatt gctgcttggt
    21481 gaccgtatgt atctatccct ttcgcgcagc cactgacgat cacatatcca gcttctatca
    21541 ctggttttag taaataataa actgtttttt tggctaaagc agaagtgtgt cttccaccaa
    21601 caacgcttaa catgttacaa gaaagtaaag atagatcacc attataaaac agtacaattg
    21661 gcggataggt caagtgaagc agctgagggg gatatttggg atcaaagcaa gtgataaacg
    21721 actgtttcga ttggattttg ccccagttag cgtgaatttc ctgccaactt ttgcgaaaca
    21781 gctcccggtg tttggttatt ttacctatct taatgatgtc ttcaaatccg atttctttat
    21841 aaggatactg catcatccag ttagcaagtg cccattttcc ttgactactg attccttttg
    21901 tataagcaag tttcaataaa ttttcttcta tttgatacat cataaaaccg cctttcgtct
    21961 ctctttctaa taagatacgt aaaagcggct gttttttatt gtaaatattt ttttacaggt
    22021 gcaaaacttt tacgatggat tggcgtaatt ccttgtcgtt caagtccttc taagtgttcc
    22081 tttgttccat aacctgcatt atgcgagaaa ccatatccag gataaagcac gtcatattct
    22141 ttcatcaatt ggtctcgata tactttagca atgatacttg cagcagcaat cgacacagat
    22201 cgtgcatccc ctttgattat tttttcctgc tgaataggca aatccaaggt catcgcatcg
    22261 atcaacaaat agcttggtac aatggacaat ttttctactg cttggatcat cgctaattta
    22321 cttgcttcat aaatattcac atcatcgatt gtctgctgat caacgatccc gatcccgatt
    22381 gccagggctt tttcttgaac ttgttgataa agaagttctc gctttttctc tgataattgt
    22441 ttggagtcat tcagccctaa tatgatctca tttttcggca agataactgc agctgccacg
    22501 acaggtccag ctaatggtcc tcttcctact tcatcaatgc cggcaatcca ttggtgacct
    22561 tgtgcgtacc cttgcttttc aaagcaaaac atttcttcat atttttcctt aattaatgct
    22621 tcctttttca atcgtcgttc aaaagaagcc agtgcttgct gcacgccttt gcgtggatca
    22681 tttcttaatg attctagtgt aggatcatct attctctgta tgcttttcaa tcgttctttg
    22741 atactttgga tggattcagt catcactttt ttgctccatt tcttcgcaac gatccagtgt
    22801 gtatcgtcca agttttcctt gacgaatatc atgcacgact aattcgctgc cacgatcata
    22861 gtcttcttta tacccacgtt ttgctgtaat atccatcagt aattccggtg ccaatttttc
    22921 ttcttgctcg attgataaac cataacgatc gctgattctt cctgaataat atcgggcaaa
    22981 aaagtctaat ccgtaaatcg ctaaatcatc taaatggaca agctggtctt tgatcgcgcc
    23041 tgtcagcgct agttttttgc cgatttcttc atcttcaaat ttgggccata aaatccctgg
    23101 tgtgtctaac aattccaact cattacctaa acgaagccac tgctgacctt ttgttacgcc
    23161 aggcttattt cctgtctgtg caattttctt gcctactagt cgattcatca acgtagattt
    23221 cccaacattt ggaataccta agaccattgc ccgaatagcg cgaggcttga tcccccgagc
    23281 agcatctctt gcccgttttt ctttcaatgc ttctttagcc gcaggtacta gacctttaat
    23341 tcctttactt tcttgtgccg tgatagctaa tgcaagatgg ccttgtttac gaaaatattc
    23401 ttgccacagc tttgtctgtt gtggatctgc cagatccgct ttattcaata aaacaaggcg
    23461 aggtttttgc tgaatgatct gatctagcat tggattacga gaagataaag gtaatcgtgc
    23521 gtctaccaat tcaaatacga tgtccacata tttcattttt tcagaaactt ctcgtcgggc
    23581 ttttgccata tgtcccggaa accattggat ggtcattgtt ctctccattc ctttcatacc
    23641 ggaatattcc ccggctttac ttgttttttt ctttttcaat ataaattcac ttttttatta
    23701 taaagaaatt ccatctatta gttaagtgaa agtgaaccag tcctcattct atcacatctt
    23761 cacttttttg atgatgcaaa tttttatttt ggtttctata tatgaatcta aatttttgac
    23821 agcgtttcct aaataagaga tgatagacga acaaaacaga aaggagacga aacaaatgaa
    23881 tagtatggtt tttgttaatt ttcctgttgc tgatgtcaat cgttccgttg ctttttatga
    23941 aaagctgggt tttaaaaaaa atgaagaatt ttctactgaa gaagctggtt caatggtatg
    24001 ggatgacact ttttggataa tgcttctctc tcatgatttt tataaaaaat ttataaaaga
    24061 taaagaaata gctgataata aaaaaacaag tggtgctttg atttcgttta gtgttgaaaa
    24121 tgcggatgaa gtaaaaaaat ttgctcaaat tgcaaaagaa aatggtggta ctttccacga
    24181 agtagatatg ggaatgcctg aagaagaaat gttttcatta gaagtccaag atctagacgg
    24241 aaatacatta gaacccgtat ggatgaagat gtaagcagat aactaatctg acgtttttta
    24301 taacaaaaaa acaaaggcgg atgatacgat aagtattctc cgcctctatt ttttttattc
    24361 gtacaatctc ttttttacag tttataaaag cagtcttctc accctttttt taattgttag
    24421 atgccagtgt atgtaaatca ccaatgtgtc atgacacatt ggtgatttta tggtatcctt
    24481 ttattattaa ctgacaaagg agagaatatt ttgaacactt tgattgcaaa agacgttttt
    24541 agattgcccg aaatgaatgc aagattgttg tcgacctctg ctcttttatt agcccttgat
    24601 gtcatcttat acaagatagc gattggtcct gcttattttc aaatcacatt cggattttta
    24661 agtatggcgc ttatgggtta tctatatgga ccgatctggg ctggtttgct tgaaatgttg
    24721 tcgaacactt taaatttcac gatatttggt tcaggaacgt ttcaagctgc ttttcttatc
    24781 acggctttcc ttggtggatt tattaatggc ttatttttat atcataaaaa gataaacctg
    24841 ccttccgtca tcgctgccca attattgatt atgatcccag tcagcctttt tatcaattct
    24901 tggctgatgt ctgttttgtt tggtgcagat ttcaaagtaa tttttctagg tagattcatc
    24961 aggaatatga tccaaatccc tattcaaata atggttgttt attcatttat caaaacttta
    25021 gaggctagaa aagtgtttaa acgagctgtt tgattttacg tactaaggta gtaaactgat
    25081 tttggattaa acaataaagc gtgcagtctc aacagactgc acgctttatt gtttctcact
    25141 ggatccattt tatttcatca agtggataat agacaaatct tgctttacct agaatatcag
    25201 ttttattgat cgtgccaaac gaacgactat cttttgaaac tcttcgatta tctccaagta
    25261 caaaataatt atcttccgga agcttggatt gtcccgttag ctcttgtagt gtaaagtccg
    25321 tcgtatagga tgcatttgca tgatctgatt tcaggttttt agttagataa ggttccttaa
    25381 ctactttccc attgatcttc aattgatcat tttggtagct gacattttct cctggaagac
    25441 cgattactcg tttgatataa atcgtgccat ctgctaattg aaaaacaaca atatcaaatc
    25501 gtctgatctc cgaaaatttt tccatcacaa ccatatcacc ttgcttcaac acgttttcca
    25561 ttgaattgcc ttccactggt accgggatga ataaaaatcc ccgcaacatg aacgcaagga
    25621 atacagaaac aagtaagtat ttgaatacaa gccagaagcg attaatatag cgttgttttt
    25681 ttgtcaatct ctatcaatcc atccctcttt ttttcattct t
//
LOCUS       DACCXM010000029        25612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCXM010000029 DACCXM010000000
VERSION     DACCXM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.031"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            163..2223
                     /locus_tag="IVY61_001022"
     CDS             163..2223
                     /locus_tag="IVY61_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970151.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2306..2875
                     /locus_tag="IVY61_001023"
     CDS             2306..2875
                     /locus_tag="IVY61_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7970152.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3050..3856)
                     /locus_tag="IVY61_001024"
     CDS             complement(3050..3856)
                     /locus_tag="IVY61_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970153.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3994..5508
                     /locus_tag="IVY61_001025"
     CDS             3994..5508
                     /locus_tag="IVY61_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP7970154.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5911..6894)
                     /locus_tag="IVY61_001026"
     CDS             complement(5911..6894)
                     /locus_tag="IVY61_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP7970155.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6899..7546)
                     /locus_tag="IVY61_001027"
     CDS             complement(6899..7546)
                     /locus_tag="IVY61_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7970156.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7631..8263)
                     /locus_tag="IVY61_001028"
     CDS             complement(7631..8263)
                     /locus_tag="IVY61_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7970157.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8279..8830)
                     /gene="coaC"
                     /locus_tag="IVY61_001029"
     CDS             complement(8279..8830)
                     /gene="coaC"
                     /locus_tag="IVY61_001029"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP7970158.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8827..9564)
                     /locus_tag="IVY61_001030"
     CDS             complement(8827..9564)
                     /locus_tag="IVY61_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP7970159.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9561..10550)
                     /locus_tag="IVY61_001031"
     CDS             complement(9561..10550)
                     /locus_tag="IVY61_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7970160.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10808..11350
                     /locus_tag="IVY61_001032"
     CDS             10808..11350
                     /locus_tag="IVY61_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7970161.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11367..12452
                     /locus_tag="IVY61_001033"
     CDS             11367..12452
                     /locus_tag="IVY61_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970162.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12464..13270
                     /locus_tag="IVY61_001034"
     CDS             12464..13270
                     /locus_tag="IVY61_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7970163.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13271..14098
                     /locus_tag="IVY61_001035"
     CDS             13271..14098
                     /locus_tag="IVY61_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7970164.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14095..15168
                     /locus_tag="IVY61_001036"
     CDS             14095..15168
                     /locus_tag="IVY61_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7970165.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15596..18292
                     /locus_tag="IVY61_001037"
     CDS             15596..18292
                     /locus_tag="IVY61_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7970166.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18369..18857
                     /locus_tag="IVY61_001038"
     CDS             18369..18857
                     /locus_tag="IVY61_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7970167.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18869..19363
                     /locus_tag="IVY61_001039"
     CDS             18869..19363
                     /locus_tag="IVY61_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7970168.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19384..20193
                     /locus_tag="IVY61_001040"
     CDS             19384..20193
                     /locus_tag="IVY61_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7970169.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20190..21011
                     /locus_tag="IVY61_001041"
     CDS             20190..21011
                     /locus_tag="IVY61_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7970170.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21062..23161
                     /locus_tag="IVY61_001042"
     CDS             21062..23161
                     /locus_tag="IVY61_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP7970171.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23158..24276
                     /locus_tag="IVY61_001043"
     CDS             23158..24276
                     /locus_tag="IVY61_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7970172.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24315..25193
                     /locus_tag="IVY61_001044"
     CDS             24315..25193
                     /locus_tag="IVY61_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7970173.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25384..25536
                     /locus_tag="IVY61_001045"
     CDS             25384..25536
                     /locus_tag="IVY61_001045"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970174.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8100 a         4455 c         5420 g         7637 t
ORIGIN      
        1 aaaagtacat aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact
       61 ctcctaatag tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt
      121 actacttcat gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt
      181 tcaaaacaac taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa
      241 cactacgtta agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg
      301 catgtacccg ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag
      361 accactgatt cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta
      421 cttcctgcat tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg
      481 ttaattccta acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc
      541 ttttccgata tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg
      601 aatcatgctt tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa
      661 aaagattatt cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg
      721 atatctgatc tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa
      781 gtaatttcaa acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc
      841 ttgtcctatt ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt
      901 cgtgctgatc aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac
      961 gcccctaaag atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa
     1021 gaaatcaaaa tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta
     1081 acttatatag actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa
     1141 gcatctgctg cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa
     1201 cttcaagcag aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct
     1261 gaattttctg atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc
     1321 acttctattt ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag
     1381 atccttgaag tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt
     1441 acctttactt atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt
     1501 catccaaacg aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt
     1561 aaacccgtac cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt
     1621 tatttgacca aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca
     1681 ctccaagaac tccatgctca gtcactatta catgttttac cattaaacaa acagatagat
     1741 caaaaagaac aaaaaagaca agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat
     1801 gcagtttctc caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct
     1861 agaaaaatct cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt
     1921 tctggattgg aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt
     1981 gttgctaaac agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa
     2041 cgcttgtcaa ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt
     2101 ccgggaacac ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga
     2161 ggtatggcag gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc
     2221 tgatttatat ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat
     2281 aatggagacg aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg
     2341 gatgacaaga ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt
     2401 tcgattttat caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga
     2461 atttcaaacg acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga
     2521 agaacttgaa agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg
     2581 taccgcttgg cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt
     2641 gaaatcagat taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga
     2701 atttcctacc ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca
     2761 ggcgattgct ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac
     2821 acctaatcca gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa
     2881 tggtgaaata agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac
     2941 ttttttgagt taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg
     3001 cagtgacaaa agtattgtca ctgccccttc tcttgttttt tagcatgttt tatcgtttga
     3061 tttttttagg aataaaattc ttgatgaacg taaatttttg gtaatcgttt cgactataga
     3121 ggtaaccaaa tacactgaaa acaactgctc caataaaatt aacaaataaa tccttcatag
     3181 tatccatgat tcccacgtct aagtaaccat tttggatgat tgttttatct gttttaccat
     3241 ctgcattttg actttcaatg attgtccgag tgatatctgg aacatgatag acagtgtttc
     3301 cgtctttact taactccaca ctattgattt gctggatgat cgtatctttt tgcatatcga
     3361 attgcagtaa tttatctgct gaaaattcca caaactccca cataacacca actgtcatcg
     3421 aaaaacagaa ggaaacaact gctagtaata aaggagtcaa gttcatggtt tttgtatttt
     3481 tattgagcaa ataaacaagg gaaaagccga cacccccagc taaaaatccg ctgaatgcat
     3541 gtagaaaagt atcccacatt gaaaaatagc cataaaaatc acttaactct cctaaaactg
     3601 ttgaactaaa aataaaagat aacaaaatga tttctagcat atttggaatc tgtatcttta
     3661 atatcttttc cagaaaataa gggatttcaa ataaaattaa tgtcagtaat aaaagcattg
     3721 tatttcccca gtgttggttc cagacctgat ccactagcat aataacgacg atgatacgta
     3781 ataaaatata gattccgttt gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg
     3841 ttttcaaatg attcataaaa taactccttc atgtatatcc ttaattatac taagaagcgt
     3901 gtgtttacca aagctatatg acttaaagaa aaacagagtt gatattgtga aaaaaatcac
     3961 ttaatatagt ataaggagaa aagggggata caaatgaaaa aattagttgc tggtttatta
     4021 acagccgttg cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag
     4081 acaagttcta gtacgaagga atctgccgaa gtatcatcag gcgcttctga acaagtatat
     4141 acagatccaa aagaaatgaa agaatcttat gatgtcgtga ttattggagc tggtggtgca
     4201 gggatggcag cagctatcga agcaaaagat gctgggttga atccagtaat tttggaaaag
     4261 atgcctgttg ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca
     4321 aaatttcaaa aagaagaagg aatcaaagac tcgaatgatg ccttctatga agaaacgcta
     4381 aaaggtggtg gaggaacgaa tgataaagaa atgcttcgat actttgtcga tcactctgct
     4441 gatgcggtcg attggctaga tcaaaacggt atcaaactgg acaacttaac gatcactgga
     4501 gggatgagtg aaaagaggac acatcgtccg tctgatggct cggctattgg tggatactta
     4561 gtggaggggt tattgaataa cgtccatgag cgggaaatcc ctatttttgt caacgcagat
     4621 gtgacagaca ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca tgttcaagga
     4681 gaagaaccaa aagaggtcac cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc
     4741 agcaaagaat ttattgaaaa atatcgtccc gatttagctg attatattac gacaaatcaa
     4801 gaaggatcaa ctggagatgg tatcaccatg attgaaaaat taggtggaca aaccgttgat
     4861 atggataaaa tacaaattca tccaactgtg cagcaggatg agggggtctt gattggtgag
     4921 gttgttcgcg gagaaggagc aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa
     4981 atggatacaa gggataaagt atctgcagca attacagcat tgcctgaaaa atcagcttat
     5041 ttgatttttg accaaggcgt gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa
     5101 ggatatgtca caactggaaa gacaatggaa gaattagcag aaaaaatcga tttaccaaaa
     5161 gaaacactag caaaaacagt tgatggatgg aatacggatg ttgcggagaa aaaagatgct
     5221 caattcaacc gtgccacagc catggaacat gacttatcgt ctccgaatta ttatgcgatc
     5281 aaaatcgcac caggaatcca ttacacgatg ggcggtgtga aaatcaatac gaacactgaa
     5341 gtattagata aagaaaacca gccaattagg gggttatatg ctgctggtga agtcacaggt
     5401 ggcttacatg gaaacaaccg gatcggtgga aattcagttg cggagatcat tatttttggg
     5461 cgacaagcgg gcatacaagc agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa
     5521 ataaaaagag gttgtgaaat cagcgttttg agctaatacc gagggtcagc tgattgaaca
     5581 ctgtttagtg aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt
     5641 aaaaacagct tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg
     5701 aacaccgttt attcggaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc
     5761 tctaaacacg tataaacggc ggaagcagaa gaaattcctt cggaaataag ctgaaattca
     5821 caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct
     5881 gtcccagcct cttttttgat cagcatgatt ttattcagtt atcttcaaaa tgatttttcc
     5941 gatgtttttg tttgcttcca tatattcatg cgcttgtttg actgcgctga attggaatgt
     6001 acggtcaatg attggccgaa tcttgccatt ttctagaaaa ggcccagctt tttgcataaa
     6061 agtttgtgcg agctctgctt tatactgatc acttctaggt gtcaataatg taccaagcag
     6121 ttggacacgt ttcccaatca atacaccaag gtctatctca ggcacgatgg tcccacctag
     6181 cataccaatc aagacccaac gtccatcaac ggctatagaa gctaaattct tttcccagta
     6241 agaagcgccg atgaaatcca aaataacatc gactccccga ccttcagtga tttttcgtac
     6301 ctcttcttga aagtcttgtt ttttatagtt gatcaattca tctgcaccaa gttctttaca
     6361 aaaggcgagt ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct
     6421 agctaattgg atcgctgctg ttcccacacc acttgctcct gcatggatca agacagtttc
     6481 gccttcttgt aattttccaa gccagtaaag tgtttgataa gcagtcaaaa aaacttctga
     6541 gattccagca gcagaaacat agtctagcgt atcagggaga aggattgcac gatcggcagg
     6601 catcacagca tattcggcat aaccacctag attgactaat ccgtatactt ttgtcccagg
     6661 agtaaaagtg gagatatcac ttttattctc aactacctct ccagctactt cgactcccaa
     6721 aatagggtag ggaggtttca aagagactga ttctcttttc ataatatctg ttcgattgac
     6781 agctgctgta tggactttga tcagtaattc gccttgtttt ggtttaggaa caggcacttc
     6841 aatttgttct aatacatcta cagaaccagg atgcttgatt gaaatggcac gcatggatct
     6901 attcctcctt tgcccattct tggaatgctg tattcaactc tttgctacct tcagaaagta
     6961 ctagagtcgt acgagaaata tccgtttctt ccactagagc ggcaagtaca gcagcaactg
     7021 tagagcgagt gatcgttttt gatgccttct cattaggctt agttaattga atggaagtaa
     7081 cttcttcatc gttagtcaat gtgactggtt gaagaatgac aaaatctaag ttagttcgtt
     7141 tcagccattc atctgcataa tgtttagtaa tataataatc tgtcatggac tcaggccatt
     7201 ttgctggttc atctgcataa acagcactga ccatcagata gcgagagaca tgcgcatttt
     7261 cagctgcgat cactgttttg attgcgccat ccaagtctac ttgcagaaga ttctttcctt
     7321 gagagccggc agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc attttttcca
     7381 cggaccaagt taaatcaaaa gagacatacg aaacattctc tttttttact aatgtttggg
     7441 aagcaacatc tctcacccct gcaaaaattt catgggaact tggagctaaa tcttttatca
     7501 ggtgtcggcc aatttgtccg tttgcaccta caacaaaaat tttcatttgg atcatccttt
     7561 cgacgtcaaa tctttccatt caatagtaac accaaaagag aaaaaaatca gagatcggct
     7621 acgatgacta tcagttagta agagaagggt gcttgaagta tcttaacaag acattgcata
     7681 ctgctgcagt cgcaagacct gcgacgattc cttcaggtat accacttgta aaaataaccg
     7741 ctagaatagc tgaataaaca gcatttaaat ttgttccgat gactttggca taatcttgct
     7801 ggaataaaaa gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc
     7861 cagctaagaa taaagaaacg gaatctgctt tttgtttcat tagtttgcgc agacctttat
     7921 aaacgaaata aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt
     7981 tccagcttcc atgttctgtt ccgatgactg ggataaacgg agaaaagaca aaagacaaag
     8041 gtgtttggat aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag
     8101 cacctagtct tggtcctaaa acgatcgaag caatgattac aggaatatgc atggttgttg
     8161 cattgattgg accgataggg ataaaaccta aaggcgtaac agctaataaa atcaaaatag
     8221 ctaaaaacat cgcagttaat gtaaagtttt ttgtattttt cataagtact ccttttttct
     8281 aagcatttct ttcattaaga gtcagcatga cctgttcgat gatcgtctga atatcagcta
     8341 aggcaccttt tccaaaatct ccacaagcta aaagagattc tcttggttca atttcctgat
     8401 agccgacaga tctcaatgtt tccagattac gctgagtgat cgggttttga tacatattcg
     8461 tgttcatggc aggggcaatc aattttggtg ttcccactgg aagcgccatt gcgactgttg
     8521 aaagaagatc gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg
     8581 cgatgagaaa caagtcagct tgttttgcta gttcgatatg attgattacc tgcggatttg
     8641 attcttgcat gacgtctgta tggactgcgc gcttcgacag agattgcaag gttaatggcg
     8701 tgataaaatg tgtgctgctt ttagacataa tcacatctac ctgggcacct aatttgacga
     8761 attgacttgt aagatcagcg cttttgtaag cagaaatact tccagtaacg cctagtaaga
     8821 tctttttcat tttgattcct ccagtctaat tttctcagta atcaatgcag caatctcaga
     8881 tttactttga gcttcttcca ctgttccatc tttagataag agatagccgt ggtgtgtctc
     8941 gccatggatt tccgtcaaat catttgcgaa aataaaatca gttttgttat ttatcagact
     9001 attttttgcg actgccagaa gagattcctt tggtacatct actaatagtt tgaaaccaat
     9061 gatttttgct tttggctgcc attcacgtag ataagaaatg atttttggtg ttttcttcaa
     9121 aacgagcaca agatgctcgg tgttagaaga gatctttgaa acagtggtat ctgtctgttc
     9181 attttccgca aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc
     9241 cagctgttct tctgaaaaac taaaagcagg agtaaaatca ctgactgcca tactgtggat
     9301 aatcgcatcg tagtgttctt ttttcgttag ttcttctaat tgtaaaaata agtccagtgc
     9361 agattcgatc gtataaatcg ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt
     9421 agtcacgtaa tcgattgtgg tatccggatt ggcagctaaa taatcagcta atgcttgccc
     9481 taatcgtcct gtcgaatgat ttgtaatagc acgtacttga tcgatttttt cagtcgttcc
     9541 tccagctgtg attagaatct tcatttatgg tcacctacat ttactagtgt tcttccttgg
     9601 tgtgttccag caagtagttt agatgctgtt tcaggcaatt gatccagagt aatctcttgg
     9661 acgaccagtt catctgcaat ctgcaaatcc gccatctgtt gccataaaga aagacgttga
     9721 tctattggta cattgacaga atcaattcca atcaactgga tgtttcgcag aataaaaggc
     9781 aagacagtcg cgtttagttt gattccccca gcattgccac ataaagctac tgcaccgtta
     9841 taagagatca atggaagaag actgcttagc tgctcgccac ctactgtgtc gatgacatag
     9901 tcgatttgct gttttcctaa agcttttgtt gtctctggca gaaattcatc aggtcgtacg
     9961 acttgcaaag cacctagact ttttagccat gtgacagact cttcttttct ggaaaaggca
    10021 atgattgact ggaatcctag tttatgtaac agggctatcg ctgtactgcc tacaccgcca
    10081 gaagcaccag taacgacaac ttttttatcg gaggtcatac cttgacggat taaagcgttc
    10141 acacacaaca tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg cagcgtcata
    10201 ttttttggta gaggaactaa ccagtctcct ggtacttttt gatattggct ataaccgcct
    10261 ggatgggtga cgccaagccc aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt
    10321 ggattgttac tttggacgac agttccggat aaatcaatcc ctggagtcat cggatatgtt
    10381 cgtatcacct gaccagactc gctcattgcc aatgcatctt tataattgac atcagaatag
    10441 gcaattttta ccaagacttc attttcagcc aactcagtaa atggcagatc actgattgct
    10501 gtatggaaaa caggttcctt tattacctga aaagatcgaa aagcgttcat tcgtgagaca
    10561 ctccttttaa aattttcttt tatcatacca taaagcctac tcttttattc ttgttcttcc
    10621 gtaaatttag acaaaaatta ggtgaaaaat aaagaatcaa acaactagaa tttattccaa
    10681 aagataaaga aatattcagc aaaaaaaatt ttacaaaggg ttgccttttt caataaattt
    10741 cgtatactaa tacgagtggt agtaaacagg aagtttacta ttagaaaact tcgattcgga
    10801 ggaaattatg gaaattggtg agaagttaag aaatttgcgc atccaaaaga atttgacaca
    10861 agaagaacta ggcgaacgca cagatttgtc aaaagggtat atttcccagt tggaacgaga
    10921 tttaagttca ccatcaatgg aaacattctt ttctattctt gaagtcttag gtgtgactcc
    10981 agaagaattt tttcatcaag aagcagcgaa tctgcaagtg gtttattcaa aagaagatca
    11041 tacggtttat ttcgatgaag aaaacgggta tgaattggaa tggctcgtgg ctgattcgaa
    11101 tgaaaaagaa atggagcctg tcttgctgac ttttgaaggt gcaggagaat ataagacgtt
    11161 tgaaccttca ttatctgaaa cgtttatttt tgtattagaa ggggaattag agttaagctt
    11221 aggagagacg gtctatgtgg caaaagcagg acagtcgatc tattatcaag cgacaaaaca
    11281 acatcaatta aggaatcatc tcaaaaaacg aacaaaagta ttgattgttg caacgcaatc
    11341 atatttgtaa tatagggggt aaaaacgtgg ggaaaccaat catttcattt gatcaagtag
    11401 tgaaacgcta tgatgatgaa acagtattaa aaaaagtaag ttttgagatc gaacaaggaa
    11461 aattttatac actgcttggt ccttccggct gtggtaaaac aacgatccta cgtattatag
    11521 ctggattcac cgaagctaca gaaggggaca tctattttga agggaaacgg ttgaatgatt
    11581 tgccagctaa taagcgacaa gtaaatacag tcttccaaga ttatgcactt tttcctcata
    11641 tgaatgtttt tgataacgtt gcttttggat tgaagatcaa gaaaatgtcc aaatcagata
    11701 tcgaaaagaa ggtaaaagat gctttgcgta tggtccagct tcctggatac gaaaaacggg
    11761 agattagtga aatgtctggt ggtcaaagac agcgtgtagc gattgcacga gcaattgtaa
    11821 atgaaccgaa agtattgcta ttagatgaac cgttatcggc gcttgatttg aaactacgta
    11881 cagatatgca gtatgaactg cgggatctgc aacaacgatt aggaatcacg tttatttttg
    11941 ttactcatga ccaagaagaa gcgttggcaa tgagtgatga gatttttgtc atgaataaag
    12001 ggaaaatcgt ccaaagtggt acacccgtag atatttatga tgagccaatc aatcatttcg
    12061 ttgctgattt tgttggggag agcaatatcg tggacggtgt aatgatcgaa gataatctag
    12121 ttgagtttgt tggtaagcaa tttgaatgtg tcgatggcgg gatgcgtcta aatgaaccag
    12181 ttgaagttgt tttacgccca gaggatttga cgatcacgac accagataaa ggaaaactag
    12241 tcgtaacggt cgatactcag ctgtttcgtg gtgtccatta tgaaatcatc tgttatgatg
    12301 aacagcaaaa tgaatggatg gtccattcaa cgaaaaaagc taaagaagga agcaaagtag
    12361 gattggcctt tgagccagaa gatatccatg tcatgcgttt caacgagtct gaagaagaat
    12421 tcgatgcacg tcttgacagc tacgaagaat aggggggaca agaatgaaaa caatgcgcag
    12481 gatctattcc gtcccttata ttttgtggct cggtttattc gtgatcgcgc ctgtattaat
    12541 gatcatttat caatcttttt ttgatatgaa tgggcaattc acgctgaata attatctatc
    12601 ttattttacg tcggggacat cgctttctat gacgatcaat tctgtatggt atgcattttt
    12661 gatcacgttt ttcacgctga tcatcagtta cccgactgct tattttttaa caaaactgaa
    12721 gcacaagcaa ttatggctga tgctgattat tttgcctaca tgggtgaatc tgctgcttaa
    12781 agcttacgca tttattggga ttttcagtgt gcacggcagc gtgaatcatt tctttgattt
    12841 tatcgggatc gggccgaaac agatattatt cacagacttc agttttctgt tcgtggcaac
    12901 gtatatcgag attccattta tgattatgcc aattttcaat gcattggaag agatgaatcc
    12961 ttctttgatc agtgctagta gagatttagg cgcgaatagc tttgagacct tccgacgagt
    13021 gatttttccg ttatcattaa atggtgtgaa aagtggtgtc caagcagtat ttattccttc
    13081 tctttcttta ttcatgctga ctcgtctgat tggcggaaat cgggtgatca ctttaggaac
    13141 ggctatcgaa cagcatttct tggttacaca aaactggggg atgggttcta caatcggtgt
    13201 gattttaatc attgctatgt tcatcgtgat gctcctgaca ggcgagaaga aaaaaggagg
    13261 tcgctcataa atgaaaaaat ttaaatggtc gtatctttat ttagggatcg tctttttatt
    13321 cctatatgca ccgatcttct atctgatttt ttattccttc aatgaaggag gaacgatgaa
    13381 cagctttaca ggatttactt gggaaaatta tgaagctgtt tttgaagata cacgtttgat
    13441 cattatcgta ttgaatacgt tcatgctggc tttcttatct gcattgatcg ccacgatcat
    13501 cgggacattt ggggctatgg gaatctatta cacgaaaaaa cgacgtgcac ggacagcgtt
    13561 gttgagcatg aataacatct tattagtctc tcctgatgtt attatcggtg ctagtttctt
    13621 gattttcttt acactactag gattcggatt aggttttcag agtgtattgc tttcccatat
    13681 tgctttcagc attccaatcg tcgtcttgat ggtcttgcca aaattgcagg aaatgaacga
    13741 ttcaatggtc gatgcggcta gagaccttgg tgctaataat ttccaagtag tcaaaaatat
    13801 cattttgcct tatctttctc caggaatcat agctggatat tttatggcat ttacctattc
    13861 tcttgatgat tttgcggtta catttttcgt cacaggaaat ggctttacga cgctctcagt
    13921 agagatctat tctcgtgcaa gacaaggaat cagtttagaa atcaatgcgc taagtgcgtt
    13981 agttttcttg ttctccatga tattggtcat tggatactac tttattagta aagaaaaccg
    14041 tccaaaacgg ctgaagaaaa acagaagagt acgcgagggg gtggcacggc tgcgatgaaa
    14101 aaattacaat ctctgttttt aggtattctc atcattattg cggtgctgct gcttggtgtt
    14161 cgtcaacttg aaaaaacaag tggcatgagt ggagcaaagg tacttaccat ctataactgg
    14221 ggagattata tcgatcctag tttgatcacg aaatttgaaa aagaatatgg atacaaagtg
    14281 aattatgaaa catttgattc taatgaagcg atgttcacaa aaatccagca aggtgggaca
    14341 aactacgaca tcgctatacc aagtgaatat atgatccaaa aaatgatcaa agaaaaacta
    14401 gtacttccat tagatcattc gaagatcaaa ggattaaata atattgatcc acgattttta
    14461 gacttggatt ttgatccaga taatcagtat tcgattccat atttttgggg aactttaggg
    14521 atcgtatata acgataaatt ctttgatgca aatcaaatta agcattggga tgatttgtgg
    14581 aagccggaat taaaagacag tttgatgctg attgacggtg cacgagaagt tatggggctt
    14641 tctttgaata gtttaggcta ttctttgaat agtaagaata tggcacagct gacagaagca
    14701 gcaaataaat tgaatcattt aacaccaaat gtcaaagcaa tcgtggcaga tgagatcaaa
    14761 atgtatatga tcaatgaaga agcctccgtg gcagtgacat tttctggcga agctgccgat
    14821 atgatgtggg aaaatgaaca tttacattat gtgatcccac cagaaggatc caatctttgg
    14881 tttgataata tcgttatgcc aaaaaccgtg aaaaataaag aaggtgccta tgatttcatc
    14941 aactttatgc tagaaccgga aaatgcggca caaaatgcag agtatattgg ttattctaca
    15001 ccaaatgaag aagcaaaagc gttattgcca aaagacattt cgtcggacga acaattctat
    15061 ccatctgatg acacgatctc ccatcttgaa gtctacaaag atctaggatc gaaataccta
    15121 ggcatctata atgacctatt cttagaattc aagatgtata ggagataatg gcaaaactca
    15181 gtaagaatgg ggacttatca acgttctaaa gagctaaaaa tcgtcctttt tctatctgag
    15241 ttttccaatc ttctaccaaa agcggatgaa acgccgcttt ttttgtgtta ttttattttt
    15301 tctaccacgt ttttctacca gagaaaagtg gacaaaacca ttgtaaaata gggcccgaga
    15361 cgggctagta aatctaagat tgaaaaagtt acagaaggat aaagtaattg gaaactatgc
    15421 aaatatactt aacccttctg taatctatta ataaaactaa tatatgaatc cagttcaata
    15481 tttgtagcta tgaaaaaaca taaaaatcat attagataaa aactaaattg gtagataaat
    15541 tattttgtca gatatctgag gcaaatattc gactatttgt gtttcaaagt aaaaattgga
    15601 atacaaatat agaatagaca tattgcagcc gatagtttat tatgctttta gacacattaa
    15661 actggaggaa aaaatgaaaa agagcgctaa agatcgagtt tttgatgagc tgatacgact
    15721 aacagggaat atgtcttctg tcactgccga gaaaatttcg aagaatctag gtatctctcg
    15781 tcagaatgcc agccattatt tgacgagatt agtggaagat aaaaaggtcg agaaattacc
    15841 gggaaagcct gttttgtgga agcctttaga tgaatatgcg gttgtagata atacggagca
    15901 aataaacgaa gcctttcatt ctgtagttgg tcatgacgga agtctgagag aggtcattca
    15961 aaaatgtata gccgctgtca aatatccgcc taacggactc agtgtattaa taaatggagc
    16021 cacgggtgtt ggaaaaagtt ttttagccac aaaaattttt gaatacgcta tccatgagca
    16081 aattattgaa aaagatgcac catttgctat cttaaactgt gccgattatg cggacaatcc
    16141 cgaactgctt tcggcaacac tgtttggata caagaagggg gcctttacgg gggctgaaaa
    16201 ggataccgat ggtttattgg caacggccaa caacggctat ctttttctcg atgaggtcca
    16261 tcgcctatcg aaagaaaatc aagaaaagct ctttctattt attgatactg gaaactatcg
    16321 tccagtcggt gaaaatgtaa attggcattc tgctaaggtt cgttttattt tcgccacgac
    16381 ggaaaaaggt gaaaattatt tattagacac ctttgatcgg cgtattcaaa ttagtgttat
    16441 gttgccaact tttgaagatc gccccattcg agaacgatta gagctgattc aattattttt
    16501 tcaaaatgaa gcggatgttt tacaaaaaga tattattgtt tcaagagaag ccttgtcttt
    16561 aatgttatct catcccttta gtgggaatat tggtaagctg aagaatatca ttaaaatcag
    16621 ttgtgcggat gtgtatagtc gaacacaaga agaaccatta acgattggga aaaatgagat
    16681 cttgattcag ttaaatatgc tggaatccga agtagaaagc ttgccgattg ctagtttatt
    16741 gataaagaaa aatcaaaaga taaaaacgga tcgaatcaaa ccgtttgatt atgattcgat
    16801 tttggaaaaa attactcagc tgataaatga aggtaatttc aatgaaaatt tttcttcgat
    16861 aaagaatgaa gtacagcatt tggtgcatca cattgataag gattcttttt tatcaatgag
    16921 tagaaaattg atgattcaga tgtttagaaa ggtctgggta tccgttttag gaaaaacgta
    16981 cggattgacg acgagtggac ctgttgcaga aattattagc agatcctatg tcatttcccc
    17041 aagctataag acagacttaa ccgggataaa tcgtacatta gctgcaaatc tacctcggac
    17101 gacttactta tgtaatcagt tcttggagca tttaccaccg ctctcagaaa aggacgagaa
    17161 ctttctaaga ctgcttattg tcgtgacttt atctgattat gtagatgaat ccatcgagct
    17221 gaagggcctg ttgcttgctc acggagatag tacggctagc agcattcaag cagtagtgaa
    17281 tcaattatgc ggcaattatg tttttgaagc gttggatatg ccagtagaga gtaatgtgtc
    17341 agagattgtc gaatcgacaa ggaatttcgt gaaacggcaa gtgaatacag atagtctagt
    17401 gctgatcgta gatatgggct ctttaaatca aatttacaca cagatcaaga acgatttaaa
    17461 aggggagtta cttcttctga ataatttgac gacttccatt gcacttgata ttggcttgaa
    17521 gatgacgatg aatgcgccat ttaaacagat tgcggaggat gcagcagaca agtattcaat
    17581 caatgttcaa tactttgaag gcttttctca aagtagcaac attatcattt cctgtatgtc
    17641 gggtttgggg atttcggaca aattgaaaga tgtttttcgt catcatttat cggatgaatc
    17701 catcgaagtt ttcacgaagg actacagaga actacgtgaa ttaatcgacc aaaatgatga
    17761 aacttatttt gacaagacaa aattggtcat cacgacttcg gatcttccac gttccttttc
    17821 aattcctaat attaatgttt atgacatctt agacggtgat ggagcagcca atttggagca
    17881 tattttgagt cctgaaatga agccggcaga atttaaccgc ttgattcagg agctagtaaa
    17941 attcttttcg ttggaaggaa ttgcggaccg tttgaacttt ctgaacccaa cgatcgtgat
    18001 gagtgaagtg gaaacagtca tttctaaata tgaaaattac tatcatttca ctttaaatgg
    18061 ccgtgtaaaa ctaaatctct atatgcatac ggcattgatg atggaacgtt tattcttggc
    18121 tcggacagaa aaatctgaag aaccgaatta tgatttgaaa ccagatgaag atgaatttta
    18181 ttatgtttct agaagtattt ttcaatcaat ggagatgaaa tataatttta aagtcaatgc
    18241 ttatgagctt tcgctgctct atgaattatt aggaccgtat attcaaaaat aaagtgtcta
    18301 aaaaagtgtc taatctgttt tttttaggtt agacactttt tttaatgtga attaacgtga
    18361 ttaaaccaat gtttttaaaa gttggcacag gaattgctta tataaaagtg aacagaaaga
    18421 aaggaattaa ccatatgaag aaaattctaa tagcgactca cggtcactta gcttcaggct
    18481 ttcttagcag tattcaatta ctgacgggga aggttcaaga gattacggtg attaatgctt
    18541 atgtggatga gtgtgatttt gaacaggagt tgaatgtatt tgtaaaccag gtgaacgagc
    18601 aggatcagat ttttgttttt accgatttat ttggcggaag tgtcaatcag aagattacca
    18661 aaaccttttt ggagaaagag attgctgtaa ctattttgtc tggctttaat ctgccaattc
    18721 tgctcgaaat agtattggca accaaggaat taacaaagga ggatattcag gaactggtaa
    18781 ataaatgcca acaggaatta aagatgacca ttcttgatga ggtacatgta gtaaatgaag
    18841 aggaagattt tttttaggag gaagaaaaat gattaaagca gtgcgaattg atgaacgatt
    18901 aattcatgga caagtagcga tgacttggac aaagagtcta aatttaaccg ggttagtagt
    18961 agcaagcgat gaggcggcta ccaacgatat tcaaaagatg acattgaaga tggctgcacc
    19021 atcgaatgtc aaagtcatta ttaaaacagt tgatgacgcg attcatttgt taaaagatcc
    19081 gcgggcgaag gacatgcgct tgatggtttt agtccctaat gtcaaagatg cggtaaaagt
    19141 tgcgaaagct ttcccaacgg aaatcgaatt aatgaacgtg gggaatgctg ggaaaatgac
    19201 accgccatcg gaagataaga aaaccttatc aaaggaagtg atgttaacga cgtcagaaat
    19261 tcaagactta aaagaattag ttgaacttta tccagatacc gtttttcagc ctacaccggc
    19321 gatggaaaaa cgttcagcta tctctatttt gagtgatttt tagaaaaagg aaggagaaaa
    19381 attatgtatg atgcatttgt agtcgctttg gccgttttta ttggagttgc tggacatgaa
    19441 atgttcggga tgtccatgct gagtcgtccg attgttgttg ctccgttagc aggagcgttg
    19501 atgggggatt tgcagacggg gttgattgtc ggggcgtcac tagaagccat ctttatgggt
    19561 gtcgtcaata tcggaatctc tagtacggcg gagccttctt tggctgctgg gctggctaca
    19621 gcgttcgcaa tcaaaatggg aggaaacgtg gatgcgatta ttcccattgt ctttccacta
    19681 gcagtattag gattacagtt aatgaatatg attttttctt ttgtatgtgg accgatggct
    19741 gctaaatttt tcaaatttgc aaaatctggt gatcaaaaag gaatcatcaa tctgcacttc
    19801 tttatgtggt ttgtccacta tgcgctttat gctcttattc cattctttgc cgttttattt
    19861 ggagcagatg ttgtcaaaag cgtcttggat aatattccag aggtcatcat gaacggatta
    19921 acagtcgctg gaaatctgct gccggcagtt gggatggcga tgctgttacg gatgctttgg
    19981 agtaaaaata ttgctgtgtg gtttttcttt ggggccgttg ttatggcgta tctcgagtta
    20041 ccattgattg caattgccgc agttggaacg attattgcaa ttgttatagc ccaaagagat
    20101 ctacaactaa aaaatatatc tgttcatgca gttcaaagcg atgctgctgt aaatgcaacg
    20161 cttgaccaag aagaggagga tttctttcaa tgaaattagc tgatggaatt tctaaagatg
    20221 agaaaaaatt ggttcggaag atgttttggc gctcgatgac tatgtatgct tcggtgaatc
    20281 cggtgacgat gggcggcggt ggtttcgcct attctatgat gcccttcatt aatcatttct
    20341 acaaaagtga agaagatcga gctgcggcgc tggaacgtca tacacagtat ttttcgacga
    20401 ccattccttg cgcgtccttt gtcatgggga ttgcggcatc gatggagaag gaaaattctg
    20461 aaaaagctgg ttttgacgtg gaaagtatca acgcgatcaa aacgagtttg atgggaccat
    20521 tggctggaat tggcgattca ttgttctggg gagtttggcg tgtcgttacc gcgggactag
    20581 cgatcaactt agccaatgcc ggaaatattt tggcaccaat tatcttttta gttatgttca
    20641 atatacctaa ttacttgatg cgatactatg gcggcgtttt aggctattca ttaggctcta
    20701 aatatattga aaagctgtat gcgaacgggt taattgaaat tctgacaaaa gctgctagta
    20761 tcttgggtct gatcatggta ggtgcaatga cgatcagtat ggtcaaattc aataccgttt
    20821 tgagcttttc aatgaagggc gaagaactaa tgaatgtcca agaaatcctc gatagtattt
    20881 ttaaaggaat tgtaccgtta ttggtaacat tgagctgttt caaattacta gataaaaaag
    20941 tcggcgtcat caaaattatt tttggaatgg tcgtcttggg aattttattg tcattacttg
    21001 gaatcgcata gtaggaagct gcagctacta ggctgcagtt tttttagcaa ggagggaaaa
    21061 gatgactatt aatgaagtaa tttattcacc caaacaccca ttttttaatg ctgagttaaa
    21121 cgacggaaat gtgactactt caaacgaata cagctactat cggaatattc ctgatgtaaa
    21181 gggattgacc ttcgatgtaa gtaatcaaaa gacattagca aaccttgatg tttgcgggac
    21241 gattcaaacg ttgacctttt atcgagaaaa tttactaact gaggaaaaac caggtgtttg
    21301 ggtcaataaa cagctctctc aaaccaattc gttatcctta aaggttgaag taaacgggat
    21361 tgtctaccat ttgtcagaat cggatcatcg tattgaagtc gatgtgattc aagactgttt
    21421 gcctagatat attcatcatt attcgacgtt taaggtcatt gtagtatcct ttgctcccat
    21481 actggctaat aagcgtttaa gtatgcttgt tcaacaggtt tacattgtga atgaaacaca
    21541 cgaaagcctt caagtttcag tgaaagatgt gccttcgtat caagagaagt attcggatca
    21601 gcaaaatgtg ttgatttctc aaaaaggaaa taaggacacg atcgcacaac aggaagtggc
    21661 aagcttcgcc attgcttttg tggacccaaa tgcttttgaa gaagtaatga cttttcagga
    21721 atctgatatc gaaaaatggc tgaaggaaac attgaactat tttgctcagc tatttgggca
    21781 gctgtcaatg ccaaacgata gagttgtcca tctctatcgt cgtgcacttt atcaaagctt
    21841 ttctagtttc ggtatgaatc gacagggaca ggtcgtcggc agcaattggg gcagctatcc
    21901 tgctactaat cgaatttgga ataaggatat gtattacgca tcattgccat tcaccatgtt
    21961 tgaaccggag ttatgtcaaa aaacggttct atggtttgat caatatggcg ttaagtttcc
    22021 tggttcaaaa tttacaggag gtatcaatca ttctctgagt aattcgttat ccagtgcgtt
    22081 actggctagt ctttattttg aacatactgg ggatacaaaa ttctttgagc ggtgtccgca
    22141 ggttctactg aatgcttcaa gaattattga ggacatatta gctcaacgtc ggtcagggga
    22201 accaatgctt tttccatctg tttggttatc agacgccttt gcacttggaa agtatcatac
    22261 cggcagcaat ctttgtcttt ggaaggcctg tattggactt gcagaaattt ttgaagccct
    22321 agaacaactg agtcttagca agcgatacaa aaacattgcc tgtaaactaa aggaatcaat
    22381 ttgtaaacaa atgactatcg atggtccttt tggtgagcaa tttttggagg gaatcggtga
    22441 tgaggagaaa acaacctatt cagtgaagca ctatcaaaaa ccgattcttg agcaagggtt
    22501 aatcttttta agtgatgtga ttgtggatgg aaaaattaat ttattgatgc atgatggaga
    22561 agaatcagac actacgttga tgccttttta tcaattttta gataataaag atcatttata
    22621 tcagaacacc atggcctttt ctgctagttc tttcaatcca acctatagcg aggaaatcaa
    22681 aggcatcact tggggattag aatccggcgc cacttttcct ggctttatca ctgttttaat
    22741 gagcgagctg cacaaccctc aagctttcgt aacccgatta gaagaactaa tttgtttggc
    22801 agatttagac ggttcgtggt ggtggtggcc gtatcgctta gaggctcaac aaggagaagt
    22861 cgtgcgagac tttggctgcg gcaaatgcgg ctgggcatcg ggtctttttg tcagcctaat
    22921 gatcagtcaa tatttcggat tgaaattcaa aaaaggagta ttgcaaattg atcctgttga
    22981 taatttaacg ttttcttgga agaatttgaa gttcggtcaa gccttcattt cgattgaatg
    23041 tacgcaatcc gagttgagta tttcaaattt gtcagaaaag ccattgaaaa taagtgatac
    23101 gaaaaaaatt cttcacgtag aaaagggaaa aacgattcac attcaaagga gaaaaagatg
    23161 agtatactaa aaaatattac ccaatttaaa aaaatccaga gtcgagcaat cactcccgaa
    23221 aatccaacag gcgagaaagg taaagctgcg atgttgccaa gcgagctagg accaagccgc
    23281 aaaggtcgtg gcgcaattcc gctgacttct ggtgaagaaa ctgtgattgc ggatattact
    23341 ggaacgggaa taattcgtca tatgtggatg acgattcgtg atcgaacaga atttggcagt
    23401 tttgttttac gtgatttaat cttaagaatt tattgggatg ggtcggatag ccctgctgtt
    23461 gaaagtcctt taggggattt cttttgtaat gggtttggag agcgttgcga ggtgaactcg
    23521 ttgccgatag tggttaatcc tacagggggc tttaattcct atttcgaaat gcctttccaa
    23581 acatccgcta aaattacgat cgagaattta catccgaagg atatgtcctc atttttttat
    23641 acgataaatt acgctttggt ggacgaattg gaagaaaata gcctatattt tcacgcaaat
    23701 tggcatcggg aacgcattac caaattacag gaggattata agttaattga tcgattaaag
    23761 ggtcaaggct attacgtggg tacttacctt gcgcttacct gcctagaacg ttattggtgg
    23821 ggcgaaggcg agtttaaatt ctacattgat ggggatagtg agtatccgac cgtaacctca
    23881 acaggttcag aagattattt tggcggggcg tgggcctttc atgagtacga tgagttcgga
    23941 cgtccacatg cgaaaaacta ttcaacagcc tttttagggt atccttacca atcaaataga
    24001 gatgccacaa gagatcgttt tgaaacggga aaattgaatg ccgttcatgc ctttggtgac
    24061 gacgcaatgc cacgacatgg tctttatcgt tggcatcttc aagaccccat ctgttttacc
    24121 gaagatttat cggtcacttt acaacaaatc ggcaacgatg atgttcgctt atttgagcgg
    24181 caagacgatg ttgctagtgt cgcttactgg tattcaacag aagcttcagg aaatactaaa
    24241 cgtcatttca ctgatcgaga acgcttgccg cgttaatgaa gtgtttttca catattcgaa
    24301 gggaggaacc tatgatgata tatggggcaa ttgaagctgg agggactaaa tttgtctgtg
    24361 cagtcggtac tgaggaatta acgattttgg aaagagtcag ttttcccaca acaactccag
    24421 ttgaaacaat gaagcaggtc attgattttt ttaaaccttt taaggaacaa ttggcaggca
    24481 tcggggtcgg ctgctttggt ccaattgata ttcaatgcaa atcaacgagt ttcggtcata
    24541 tcacatcaac accaaaactt gcttggcaaa atttcaattt tgtaggaacg ctaaagcaat
    24601 atttcaatat tccgattgaa tggacaacag atgtcaatgc ggcttgctat ggtgaatatg
    24661 ttgctggttc tggcaaaggg ttagcaggtg ttgcatactt cactgtggga acaggtatcg
    24721 gaggtggtgc cttgttcaat gggactttcg tggaagggtt cagtcatccg gaaatggggc
    24781 atatgttgat caagcgacat ccaaaggaca atttttcagg aaactgtccc ttccaccaag
    24841 attgtttaga ggggatggca gcaggaccag cgattgaaaa acggttgggt gtaaaaggcc
    24901 agaatttatt ggctgacgat tcgttttggc agattgaagc cttttacctt gcccaatgtg
    24961 catacaatac aacactgatg ttttcgccag atagaattat ttttggcggt ggtgtaatga
    25021 aacaagaaca catgaagaaa aaagttcagg ataaatttgt tgagcttata aatggatatg
    25081 ttgaaatacc accaattgat agctacatta ttacgcccga attaggtgat aatgccggaa
    25141 taatcggcgg attagcattg gccagaaaag cggtaagaaa taagcaacca taattaatga
    25201 atgtttcatt gatactcatg tcaaaagtat ttaaccgagt aatcaaatga tcaaatgaag
    25261 ttggataaag cgaaaatgaa ttcaaataaa aaggctctaa gtcaaatcgt gttgttttca
    25321 ttaaatcact gaaaggaaat gcttctgcgt ttcctttttt ctttttgttg tgcgctgtat
    25381 accttgagga tattcactgg tggcgtacac ttaaatagta atatattgtg ttactttatg
    25441 tctgtggtaa tagttatagt tgcttaccaa tgtatgttca tttttcgaaa tgttgacttc
    25501 ataatccttg gcttaatgtt tgaatttttt tggtgataga ttttttcagc cgtataaaat
    25561 aaatcgtaat tctattatag agagttgtga ttataaaaag atagcaagaa aa
//
LOCUS       DACCXM010000030        24426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCXM010000030 DACCXM010000000
VERSION     DACCXM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.032"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(5..1279)
                     /locus_tag="IVY61_001046"
     CDS             complement(5..1279)
                     /locus_tag="IVY61_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7970175.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            1552..4023
                     /locus_tag="IVY61_001047"
     CDS             1552..4023
                     /locus_tag="IVY61_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7970176.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4126..5862)
                     /locus_tag="IVY61_001048"
     CDS             complement(4126..5862)
                     /locus_tag="IVY61_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP7970177.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5859..6563)
                     /locus_tag="IVY61_001049"
     CDS             complement(5859..6563)
                     /locus_tag="IVY61_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7970178.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6694..7197
                     /locus_tag="IVY61_001050"
     CDS             6694..7197
                     /locus_tag="IVY61_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP7970179.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7157..7495
                     /locus_tag="IVY61_001051"
     CDS             7157..7495
                     /locus_tag="IVY61_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP7970180.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7516..8934
                     /gene="ffh"
                     /locus_tag="IVY61_001052"
     CDS             7516..8934
                     /gene="ffh"
                     /locus_tag="IVY61_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP7970181.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9046..9624
                     /locus_tag="IVY61_001053"
     CDS             9046..9624
                     /locus_tag="IVY61_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP7970182.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9628..10149
                     /locus_tag="IVY61_001054"
     CDS             9628..10149
                     /locus_tag="IVY61_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7970183.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10228..10782)
                     /locus_tag="IVY61_001055"
     CDS             complement(10228..10782)
                     /locus_tag="IVY61_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7970184.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11035..11310
                     /gene="rpsP"
                     /locus_tag="IVY61_001056"
     CDS             11035..11310
                     /gene="rpsP"
                     /locus_tag="IVY61_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP7970185.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11322..11573
                     /locus_tag="IVY61_001057"
     CDS             11322..11573
                     /locus_tag="IVY61_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7970186.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11698..12489
                     /locus_tag="IVY61_001058"
     CDS             11698..12489
                     /locus_tag="IVY61_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970187.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12659..13180
                     /gene="rimM"
                     /locus_tag="IVY61_001059"
     CDS             12659..13180
                     /gene="rimM"
                     /locus_tag="IVY61_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP7970188.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13170..13931
                     /gene="trmD"
                     /locus_tag="IVY61_001060"
     CDS             13170..13931
                     /gene="trmD"
                     /locus_tag="IVY61_001060"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP7970189.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14067..14414
                     /gene="rplS"
                     /locus_tag="IVY61_001061"
     CDS             14067..14414
                     /gene="rplS"
                     /locus_tag="IVY61_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP7970190.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14787..15449
                     /locus_tag="IVY61_001062"
     CDS             14787..15449
                     /locus_tag="IVY61_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970191.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            15528..18755
                     /gene="fss3"
                     /locus_tag="IVY61_001063"
     CDS             15528..18755
                     /gene="fss3"
                     /locus_tag="IVY61_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP7970192.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18869..19183
                     /locus_tag="IVY61_001064"
     CDS             18869..19183
                     /locus_tag="IVY61_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7970193.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            19196..19570
                     /locus_tag="IVY61_001065"
     CDS             19196..19570
                     /locus_tag="IVY61_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7970194.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            19580..19924
                     /locus_tag="IVY61_001066"
     CDS             19580..19924
                     /locus_tag="IVY61_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP7970195.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19995..21347
                     /locus_tag="IVY61_001067"
     CDS             19995..21347
                     /locus_tag="IVY61_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7970196.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            21407..22549
                     /locus_tag="IVY61_001068"
     CDS             21407..22549
                     /locus_tag="IVY61_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP7970197.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            22574..22828
                     /locus_tag="IVY61_001069"
     CDS             22574..22828
                     /locus_tag="IVY61_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970198.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            23084..24268
                     /locus_tag="IVY61_001070"
     CDS             23084..24268
                     /locus_tag="IVY61_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7970199.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            24283..24402
                     /locus_tag="IVY61_001071"
     CDS             24283..24402
                     /locus_tag="IVY61_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7970200.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
BASE COUNT         8056 a         4355 c         5243 g         6772 t
ORIGIN      
        1 ttttttagtt ttcaaagatc agaccttgtt gtaaatagat ccgtgcccga aagttataaa
       61 agttgcgata accaaacgca attcgtttaa tcagcttgat cttgttattc agcccctcag
      121 tgacgccgtt tgagtgggac accttaaagg catttgaaat acccgattgg tagcaatgga
      181 agatcgtgaa tttcttttta aacgactgag gtaacgtaga tgaaattgtt ttaagggttt
      241 cttgaaagcc ttccgtacct cgtttaacga aataatagcg taataattga caaaaatgat
      301 acgcagttcg caattcttcg ttgtagctta acagctcatc aacgatttct gtttgactaa
      361 ttggtcgttt aaacagtcga tgataatgcc gtttgagtgg ctcccatgtc gtggaatctt
      421 tgagtaagag tttccaatat cgcttgagcc gacggtactt cttctgattt tcagagtgtg
      481 aggttcgaaa acgattcatg atctggacac gtaattgatt gaagctgcga ttgatgtgtt
      541 ggacaatatg aaagcggtca gttacgatct ccgcatttgg aaagactgtt ttaagcaact
      601 gacaatagct ggcattcatg tccatcacta ggtacttgac ctttaaacgg gcttttctag
      661 gaaagtgcag gaagtattga gttagtttgt aaagtctgcg atcttcaagg atcgtaaata
      721 gctgatgggt tgtcccatca acacaaataa aactcatgga accagaaaca gacttcattg
      781 atttgaactc atccatacac aacaccgtcg gaaggaagtt catacgtgac tggtaatgct
      841 tcgttgcttc acgcaagatc cgtgtcaccg tcccatccga gacgaagtac ttcttagcga
      901 ttagtttacg tgattgattt tcgtacagtt ccatgagaat tgcctgcttc agttcctttg
      961 ttagttgatg atgttcatca actaagtccg ttttcgcaga aaaggtcctt ccacaatcat
     1021 ggcagagaaa acgggtacgt tttaattcta aaatagttaa cttttctttg actttcaata
     1081 tctgagtgcg tgtcgtatag gaaccgttgc ggataatacg aaccgatcga cagtgttcac
     1141 aataggaagg agagtcatcc aattttcctt taataaactg cgcttgaacg cctttatatt
     1201 ctttttcaat cagccattct tcatcaaaaa ataaatgttt atctgttaat ccgagtaatt
     1261 ttctagtatg attatccatt gagagactcc tttggtagga tgacgttttg gtcgactaaa
     1321 ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc
     1381 gccttcgatt ttccaccaac acaaaatatt atagaaccct ttttttttgc tatatgacag
     1441 gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt ccatatctgt tctatactgg
     1501 ataggagaag tttggaaata tccagaaaac gaaatcaatc gaggtagcca catgaagaat
     1561 aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc
     1621 cgtctttttc catcgtataa agaaggatta acggcagatc aagtcaagga acgaatagaa
     1681 aaaggtgcag caaataattc agtcgatccc acattcaaaa caaatcagca aatcgtattg
     1741 gaaaacatat ttacgtattt caatttgatc tttcttatct tagccatttt gctttgttta
     1801 gtagaatcct ataagaactt gactttttta ccagttatca tagccaatac aggaatcgcc
     1861 atttttcagg aaattcgatc caaaaagatc ttggacgaac taaatgtttt aaatacagca
     1921 aaagtcaatg tcatacgaga cgggatagaa caaacaatag atatagaaga ccttgtaata
     1981 gatgacattg ttattttaga gactggatat caaattccag cagatgcaga ggtcgtggaa
     2041 gggaaactgc aagttaatga ggctttattg acaggcgaag cagatgaaat aaccaaagaa
     2101 ataggtgatc cattaatgtc cggcagtttc attgtttccg gaaaagctta cgcccgatta
     2161 gacaaagttg gagcagattc gtatatttcc cagctgacag tcaaagccaa gacaatggga
     2221 gaaggagaac agtcggagat gattgcttct cttaatcaat tgatcaaatg gattgggatc
     2281 atcattattc ctatcggtat cctgcttttt tctcaaagtt acttttttaa tggaaacacg
     2341 atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg gtatgatacc tgaaggactg
     2401 tacttgctga cgacgattgc attagcatta agtgcgacta aactagcaaa gcaaagagtt
     2461 cttttacaca atatgaaaag cattgagaca ttggcaaggg taaatgtcct ttgtgtagat
     2521 aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa aaatgatcgt ctctaaaaaa
     2581 caagaaataa tcagtcagtc agatcaattg gaagcatgca ttgctgacta tgcaaaagcg
     2641 atggcaggag acaatgcaac aatggaagct gttaaagcat attttacaaa aaccactgat
     2701 caatcctatc accatgttat tcctttttct tctgtccaaa aattcagcag tgtagcgttg
     2761 gcagacaagg tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca attgccggaa
     2821 tatgcggaag agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg
     2881 tacgatggga ttcttgaaga accagtattg acagaagcag tcacaccatt aggctatatt
     2941 ctgattgcta atcctatcag aaaagaagca agagccacat ttgattactt caaaaaacag
     3001 caagtagcca tcaaagtgat ttctggagat aatccgctca ccgtatccaa tgtagctgtc
     3061 caagcaggga tttcacatgc tgatcagtac gttgatgcat ctaaattatc agaagaagaa
     3121 tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga acaaaagaaa
     3181 atcttcgttc aattgataaa gcgtaacaat acagtagcta tgacaggtga tggcgtgaat
     3241 gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg aaatgaagcc
     3301 gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat gccagaagtt
     3361 gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt gtttttagtg
     3421 aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct gacctatcct
     3481 ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc atctttcttg
     3541 cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa
     3601 aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt gatctgcggg
     3661 gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct tttgattgca
     3721 gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag aagtcagatc
     3781 attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa gctgttttca
     3841 atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt ctcggcagat
     3901 tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa
     3961 gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat cgatatttaa acgagaaaat
     4021 tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc
     4081 aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca ttttcggaat
     4141 ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg taccattaag
     4201 gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg aatgtcggct
     4261 tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct caatgtcgat
     4321 acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat agcggataat
     4381 aatctcgctg tcgctttttg aatattggac agcattttca attagatttt tgataattgg
     4441 ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa
     4501 ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat aaggttctgt
     4561 ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga tgatttcccg
     4621 aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt
     4681 ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca gtgaagtcac
     4741 aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct tttctaaacg
     4801 tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt ctgaattagt
     4861 aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga tctcctgatg
     4921 gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg gctcttgaat
     4981 gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta gctgcatctt
     5041 tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc caatcatcag
     5101 ttcgttcagt aacgtatata attgctcttc tgtggtagta cacgcccgat acatcttact
     5161 catttgttcg ctcaacgcat taatcgtctg atacaattct tcccattgat cgggtgtttg
     5221 catgattact tccgcctgat taggattttg caccattttt tttagtacgg gcagcacggt
     5281 tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa gaacaaggaa
     5341 gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg gtaaaaaacc
     5401 gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt tgactggtaa
     5461 tgcgacatat aaaagatcct cattcaaagt agtacttttg cgcaaagcgc tacctaggtt
     5521 cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat tcaatgtttc
     5581 attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc gttcgttcga
     5641 ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt tttcgacatc
     5701 taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct gttccacgat
     5761 ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac taactaaaag
     5821 aataagaaag aagagacgtt cataaatttt tttccatttc atcgtttggg ttcctgaaac
     5881 ttatagccaa atcctctaat agtcaccaag tactttgggt gttttggatc tcgctcgatt
     5941 ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc gaagtcaaaa
     6001 ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa
     6061 taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc tacggtcact
     6121 tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg ctcagcttca
     6181 tcttttgatt gcttacgagg ttcaattcgt ctgaatattg ctttcatacg ggctaatact
     6241 tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag gccaatgatc
     6301 cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt tttttcttga
     6361 cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc taaaatgatg
     6421 aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt ggcactggtt
     6481 accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga aggttcatca
     6541 tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt ctgtttatat
     6601 tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt ataataggaa
     6661 ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc atgtttaggc
     6721 ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat ggaactagtt
     6781 gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc aattcctaga
     6841 gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta
     6901 tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat
     6961 caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag cccttcatgc
     7021 ggaacccatt cgatttatga cggaagtttt tccggaataa aaaaaaatgg tgccggagtg
     7081 gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa
     7141 actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt
     7201 ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt atgcagatga
     7261 tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag tatatgacaa
     7321 tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaactccatc ttttttctaa
     7381 ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag ataagtaccc
     7441 ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag aataaataaa
     7501 aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca ggcgatggga
     7561 aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc
     7621 cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt tactaagcgc
     7681 gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc
     7741 gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc agagttaaat
     7801 aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc aggtaaaacg
     7861 acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg tccgctccta
     7921 atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt aggtcaacag
     7981 ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat cgtccgtcaa
     8041 gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac ggcaggacgt
     8101 ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca
     8161 aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa cgtagctgat
     8221 agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga tggggatact
     8281 cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa attcatcggt
     8341 tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc tagccgtatc
     8401 cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta cgacgagaaa
     8461 aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt
     8521 gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa aatgatccca
     8581 ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc
     8641 aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga tctgttgaac
     8701 ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga agtgaatcga
     8761 atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa aggggatatg
     8821 aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg
     8881 gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa
     8941 aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta tcagcaattt
     9001 ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag atgtccgtgg
     9061 agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt tcctgaatat
     9121 gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg attgagctgg
     9181 cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt cgatatcaaa
     9241 aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa aggaattatt
     9301 cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt gaaaattcaa
     9361 gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa aatcgttgat
     9421 tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga tgaattagca
     9481 aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta tgctttttta
     9541 gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga agaggtcgta
     9601 cgtatcggca gaaagggcga ataaagcatg cgaaaaatca tattttatgg tgctataagt
     9661 ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga cactccaaca
     9721 ggtaagcaaa ctacttatga agctttttat gaaacaatcg atacgaccat catgggcaga
     9781 aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc agagaaaaag
     9841 aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt cgtaaacgaa
     9901 gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat ctgggttgtg
     9961 ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga atggtatatc
    10021 cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg aacatatgaa
    10081 gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt gcattatgta
    10141 cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc ctgtacatag
    10201 ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa aatctagata
    10261 cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac cattttcatc
    10321 aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg gcacattcaa
    10381 aaagacaagt gattggcgta gatggtggtt tgcgccaaag ccgccgatcc cacctggtga
    10441 tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag gtcttgagcc
    10501 tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag gagtaacgaa
    10561 aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag caggagcctg
    10621 ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta caaatacact
    10681 ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt tgtaagaatc
    10741 ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt tcctccttca
    10801 aactatctat aataatctta caaaagataa gtaactggag caaataaaaa cacttactga
    10861 caaaagctac atagaagata gattacttat ttttaacaaa aaagaagtct aaaatcttct
    10921 attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa
    10981 aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata aataatggca
    11041 gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg tatcgtcgta
    11101 gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct
    11161 ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg gttatcaaaa
    11221 ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa
    11281 caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat ttaagcgact
    11341 tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag ttagacgtcg
    11401 tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat attggccgta
    11461 tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac ggtgttcgga
    11521 tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag taaataaacg
    11581 tgctgtactc gaattttcga gtgcagcttt tttgtttttt ttattaattg aagaaatgat
    11641 tatttggcaa aacaagcaag aaagcgtatc cttataggag gagggagtga acagccaatg
    11701 agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag ttcggttact
    11761 caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac cagtccagaa
    11821 aaaagtagta ccaaaacggc ttccaccact gaacaaacaa tggctacctc aaataatgag
    11881 aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat gcctctagat
    11941 gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca aggttattct
    12001 attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa
    12061 acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga aacaatccaa
    12121 gttttgcagc catttgagat cgacacaaat ggtcagcaag tggacttagg ttctcgaatc
    12181 acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga aggaaactgg
    12241 cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt agctaaagaa
    12301 atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg taaaattact
    12361 gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat
    12421 gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt ttctatgaag
    12481 aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc acaggaagta
    12541 atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa aagaaaacta
    12601 gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg gatataagtt
    12661 gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg
    12721 tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg tgctaacact
    12781 attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa
    12841 ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa aatatcggga
    12901 tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg aattctatta
    12961 ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta aagtaaaaga
    13021 aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat
    13081 attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg
    13141 tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact ttatttccaa
    13201 gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat aaaggattat
    13261 tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag acagtagatg
    13321 attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc tatgataaca
    13381 tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt cttgacccag
    13441 ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa cacttagtat
    13501 tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg acagatgaag
    13561 tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc atgatcgatg
    13621 caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa acggattctt
    13681 attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat aaagggatga
    13741 aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg caattaaaag
    13801 aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt gaatggacac
    13861 ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa acagcagatg
    13921 aagccaagtg actttttcta aaatcttctt tacagtaggt tgataatatg ttagtatttt
    13981 atggtgagtg caaaagcact taactattac gatattccgc tgtgagaaaa gctcataaga
    14041 atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta acaaaagaac
    14101 aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag
    14161 ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc aaacgccgcg
    14221 gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg ggcgttgaac
    14281 gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc tacggtaaag
    14341 tacgtcgtgc aaaattgtac tacctacgtg cattacacgg aaaagctgct cgtatcaaag
    14401 aaatcagaag ataaacacgg aaaacttcct tggaaatctg aaatttgtga aaagtccttg
    14461 tatatcaagg gctttttctt ttttctctat cacagtttta agccattttt ggctgttttg
    14521 gaccattttt caatcatatt cccttaaaaa ttgtcctaga ttgtccgaca ttgactatca
    14581 gtgtcgtcaa aatagggcaa aatttagtag tcagcttgac gacaaatata atacgaaaaa
    14641 taccaaaaca tttaccgaat ttagcaactt ttgaacccaa gtttctcaaa gggtcagcta
    14701 ttagcaaaaa agacaaaaca tgtacttttc tactaaaaag ttgatgtgat aggcttctat
    14761 ctaaataact attaggagtt tttatcgtga agcaaaatga tggacgtatc atttggaaaa
    14821 atcaaagtga gttaaaactg attttaacaa ttaatgaatt catcgaaaaa cacggaatta
    14881 catctagccg tcaataccag aaaaagctat cagaaaatcc aaattctgca ccaagtatgt
    14941 ggttcattaa caaaaaatat ggatcatggg agaatttatt gattagtata ggacgagaaa
    15001 atactgggta tggaaaatgg gctagaatgt cagaacaaga attattggaa atagtagaag
    15061 cctttattaa gtgcgagaag ataacctctc aacgaatgta cgaacaaaaa tcagtaggga
    15121 agaatattcc ttcacttagt accattaaaa agatgttagg agatatacgt ccacttttta
    15181 aagaaaaaaa tgatggatct cgatttactg atttcgaact gctgttagaa ctaaaaaatg
    15241 aaataatccg attaaaacta caggatgatt tatcaatgac aaagtttcga aaactagttc
    15301 aatccccaaa actaccatca gttgatacaa ttatgaaaag aacgaacaaa aattgggagg
    15361 aattgatggc agagattgga tttgattatc gaagaatcaa aatttataag cagagaaaca
    15421 acctatccaa gacaaagaaa actaaatagt atcactctaa atcagacgtt caaaattaaa
    15481 atttggacgt cttttttatt acccaaaaac gaaaggagat ttattttatg aaaaagccaa
    15541 gatttaagaa ttggcgtttg ctcgcaacac tgagtctatt atgccaaaca attggtggat
    15601 cgcttggccc aacgattgcc tttgctgatg aaattactca tccacaaacg gtaactgtgg
    15661 aattggattt ggctcaccag tatgctgtcg aaggtacatt tagcgatggt cgtcccatgt
    15721 cagaagttac cgttccgcac tatgcagttt acaatggtgt gaagcaagat attttctgta
    15781 ttgagccagg ggtgccgatt tacaatgagt tcactcctgg ttatgagaaa aatccattgc
    15841 ctgatatgtc ggagaaagcg aaattagttt ccgttttatg gaaggaagct ggtacggatg
    15901 tggatacaca tattgtggcc cagaagatga tttggcaaga agttaatggc tacacgcttc
    15961 actcaattaa acgttctgat ggtagtgcag taaatatcgc agccattgag tcaaaaatca
    16021 atcaagccat tgcggattat caaaagaaac caagcttcca taactccaca gtgaaaaccg
    16081 tgttaggtca atcgactact gtgacagata cgaacggctt gaatttatcg gagtttgatg
    16141 aagttgtcga gaatatcgca aatattgatt atcgtgtgaa cggcaatcaa ctagtgatta
    16201 ctccaaatgc caattcaaaa gagagtggtg aattaaccct aaagaagtca gctaatacag
    16261 gaacaccggt agcttataaa atggctggtc aacaaacttt gatggcgggg gcaattgata
    16321 agcccaatac ctacacaatc aaaattgatg tggaaactga gggttctttg aagattaaaa
    16381 aagtcgataa agaaacagga aatgttgtac cgggaaccgt tttccattta gactttggaa
    16441 aaacattacc tgcaaaagac gtgaccaccg ataaagaagg cattgcgaca ttggatgaga
    16501 ttcctcatgg cgcgaaagta accattactg aaaaatcagt gccagctcct tatacgattg
    16561 acactacacc aatcaccgca accattaaat ctggtgagac tatttctgtg acttcaaaaa
    16621 atactcgcga aaaaggtcaa atcattttga ataaaatggg ggtggaaaca ggaactgatc
    16681 tttggaacga caaatactca ttagccggaa atacgtttgc cattcgcaaa gacagtccaa
    16741 ccggtgaaat tgtccaagaa atgaccacgg atgaaaaagg tcatgcggaa acaccaaaag
    16801 agattgccaa tgcgttggaa ttgggaacct attatgtgac cgaaaccaaa gctagtaatg
    16861 gctttgtgaa tacctttaaa ccagtaaaag tcgaattgaa ctatgtcaat caaaccgtgg
    16921 ctcttgttac cagtaacgta aaagggcaaa accaagaaat cactggtgaa accactttga
    16981 caaaagaaga caaagatacc ggtaatgaaa ctcaaggtaa agctgagttt aaaggagctg
    17041 aatatactct ctttactgca aaagatggtc aagcggtcaa atggagtgag gcttttaaac
    17101 cagagttagt gaagggaacg aaagcttctg atgaaacggt gactttgact ttagatgaaa
    17161 agaatcaagt tgctgttaaa cacttagcga ttaatgagta ttactggcaa gaaaccaaag
    17221 cacctgaagg atataccttg gatgaaacaa agtaccctgt ttccatcaaa aaggtggatg
    17281 ataacgaaaa aaatgccgtg attacccgag atgttacggc aaaagaacaa gtcattcgct
    17341 ttggctttga tttctttaaa tttgctggat cagctgctgg gactgccgaa actggcttca
    17401 atgatctgac ctttaaagta tcgccattgg aaggaaccag tgagattaca ggtgccgaag
    17461 atgaagcaat tacagcttat aacgagcaac taggttttga tggctatggc aagtttgaaa
    17521 atcttcctta tggggattat ttacttgaag aagtagaggc tccagaagga tttcaaaaaa
    17581 ttacgccatt agaaatccgt tctactttta aagaaaataa agcggattac actaagagtg
    17641 aatacatttt tacgattacc gaaaaaggac aaaaacaacc aattaaaacg gtgaccgttc
    17701 cttacgagaa actgacgaat aaagcatttt ctgttagctt gaatcgtttg atgctctatg
    17761 atttacctga agaagaagat agcttaactt ctcttgggac ttggaaagac ggaggcaaag
    17821 aattgacaga ccttgattct accgagctag ttgataaatt gagctataac ttgcatgaaa
    17881 tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt ggaagcgaca aaagctgccc
    17941 aagaaaaaga tgaaaaagcc aaaccagtgg taattgccga aacaaccgca acgttggcga
    18001 ataaagagaa aacaggaact tggaaaattt tgcataaatt aaccgcagaa caagttttgg
    18061 ataaaaccat cgtcttattc aactatgtgt atgaaaacaa gaaaccctat gaagcaggtg
    18121 atgatccagt agcgaaagat gctagcttga acaatcaagc ccaaactgtc aattgtacgg
    18181 tggaacgcca tgtttccatc caaacaaaag cccacctaga agatggttcg caaaccttta
    18241 ctcatgggga tgtggtggat atgttcgatg atgtgtcgat tacccatgat gtactggatg
    18301 gctcaaaaga agcttttgaa acaattctgt atgcattact tccggataat acgaccaaag
    18361 aaatttggaa atctggcaaa attgattatg aagtgaatga caaagaattc actaaaaccg
    18421 tacttgcgga aaaagtggac acaagtaaat atccagaagg tacaagtttt accttcaaag
    18481 aaattaacta tggcaaagac ggaaacatca atgggaaaca caatgaggat ctaaaagaaa
    18541 aaactcaaac tttaacacct aaaaaagttc cgacaacacc aagtgcacca gaacaaccgg
    18601 aaataccaac tgttccaagt gactctcaag aatctagtcc tacagtaaag acattcccac
    18661 aaacagggga gaaaaattct aatgttctgc tgtttattgg gtttaccttg atctttgcga
    18721 ctgctgggta ttatttctgg aatcgccgaa actaaggtga tgtgatgaaa caactaaata
    18781 ggaaaaacca acgtgcgcga ccgcctccta aaaaaagtgg tcgcgctatt tttgtatcca
    18841 aaactaaaaa tgaaaagagg aaaagaagat ggaattgaaa tttgttgtgc cagatatggc
    18901 cgaaaccttt ggaaagataa ggtatgccgg cgaaggcgaa gttttaactg aaggatatgg
    18961 gcggaatact acagtaattg gtcgtagtta tcatttgtat tccagtaagc aacgagccga
    19021 tgatattgaa gtggtggtag ctgcggaagc cggcgaaaag gattttgatc aagatcaacc
    19081 actaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga atcgtgggtt
    19141 taccgactac gtgttgtatg tcgatgattt agtgaaagca taggaggaaa agaagatgag
    19201 attaacagaa ggtattgttg tagattcagg actaacgttt gggaaattac gattttccgc
    19261 attacgccga gaagtacgga agcaaaatga ggatggaacg gttagtaacg aggtaaaaga
    19321 acggacttat aatttgaaat cctctgcgca aggtcgaatg atccaagtta gtatcccagc
    19381 aaatgtaccc ttacgcgagt ttgcctatga cgcagaggta gagttggtca atccaatcat
    19441 ggatacggtc gccaattatg ttttccgaga aggaactact gtcaattggt tcattaaggc
    19501 cgatgattta gtcttgaaac gacaaccaga tcagggaaat tctacaaacc aaaatgaagg
    19561 aaagaaatag gtgaagcgta tggaaattaa tgttgaacat attttagatt gtctcgatca
    19621 atatggaaaa ggtgagctaa cggaggaaca actcactaaa gcattaacct atgatgaaaa
    19681 aatgtttctg attatgcatc aagggttact ggaagtaggt aacgatgtcg aagaagattt
    19741 tgatgtactg aattggttag gggaagaaga gtccttcttt atggttatcg aagtaaatga
    19801 aagtctatgt cgagaagccg aaaccgtatt ggaagaaatt ggtgtggaaa tgccggatgc
    19861 cattgaagtt tttttaaagc aactagtgga aacgaaacaa ctccctatag cagtaaatga
    19921 ctagcataac tgcttgaaat tggatcaatg aaatcctatg aagctttttt cgattggagg
    19981 tggaaaccat aaaaatgtac aaagggcatc gtatacgggc aggagatcag cacttggttt
    20041 atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc gtcttttatt
    20101 ttcaagagct tagacaattt gatatttcta agctatcact ttctactata gaaatcgttc
    20161 gttctataaa agatttaatc tatttgctag gaagtctcgt tctttcagga agcacgatgt
    20221 tgctttatat acactttttt caggatcatt ggcgcagttt gtggcatcga caaaagctag
    20281 cccggatgat tctagaaaat cattggtacg aagtaaaaca aactcagagt gaaggatttt
    20341 tcaaagacct caatagtagt cgaaccaaag agacaatcag ttacttcccc aaaatctatt
    20401 accgtatgaa ggatggttta ctttctattc gcgttcaaat ttcactggga aaatatcaag
    20461 atcagctttt aaagctggaa aagaagttag aaagtggtct atattgtgaa ctagtagaaa
    20521 aggaactcaa agactcctat gtggagtaca ctttactgta tgatataatt gccaatcgaa
    20581 ttgggataga cgaagtagtg gcagaaagtg gggctttgcg attgatgaaa aatcaagtat
    20641 gggcttatga ttctttacct catatgttaa tcgctggcgg cacaggtggt gggaagactt
    20701 actttttact cactatcatc gaggctttat taaaatctga tgccgagctt tttgttcttg
    20761 atcccaaaaa tgcggactta gccgatttag gtacggtaat gcctcatgtc tattcccaaa
    20821 aggaggaaat ttctgcttgt gtggaagatt tctatgagcg catgatgact cgtagcaagg
    20881 caatgaaaga aatgcccaac tacaaaacag gagagaatta tgcgtttctt ggacttccac
    20941 caaacttttt aatctttgat gaatacgtgg tttatatgga aatgttaacg acgaaggaaa
    21001 gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt aggtcgtcaa tctggtttct
    21061 ttctgattct agcttgtcaa agaccggatg cgaaatattt aggagacggg attcgcgatc
    21121 aatttaattt ccgggtggct ttgggtcgta tgagtgaact gggatattcc atgatgtttg
    21181 gcgaggttga taaaaaattc tttatgaagc gtatcaaagg tcgaggctat gtagatacgg
    21241 gagggagtgt gattagtgag ttttatacgc cacttgtacc aaaaggatat gatttcttaa
    21301 gggaaattag cgacattggt ggattaaacg ttaatacgga aggataaaat aatagtatgt
    21361 agcctaggag tgtcaagaat cctgggcttt ttttacgagg tgatttatga catttttaga
    21421 tttattcgct gggattggtg gcttttgctt aggtatggaa caagcgggtc accagtgtat
    21481 cggcttttgt gaaattgatg attttgctcg ccagagttat aaggcaatcc atgatacaag
    21541 taaggaggtg gaaatgcatg acatcacaag tgtatcagac gagtttattc aatctctcgg
    21601 accagtggat atcctttgcg gtggatttcc gtgccaagct ttttcaattg cgggaaagcg
    21661 gcaaggattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcgccgctat
    21721 tctccaacct aagtttttat tccttgagaa cgtccgggga ttactcaatc acgaaggagg
    21781 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgatg tggaatggca
    21841 agtgcttaac tcaaaggcct acgttcccca aaaccgtgag cggatcttcc ttatcggaca
    21901 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggatcgtctc caacatctga
    21961 tcaaaacatc agaaacttat taaatatcaa cccttccaat cgaggaatgg ggggacaagt
    22021 ctatgggtca gatgacgtag cgcctacctt gaccggtgat gaaggaatca aaatcgcatt
    22081 gccggtagat gatggaattt ctgtagcggg tatgttactg ggaaattttg aacaaggcaa
    22141 tcgggtttat gagataactg gaacagcgcc tactttgtca acgaaacaag gcggaacgaa
    22201 gatcatgctt cggaaaaacg aaacagaata tcaagaggtg aagccaggga acagtgtgaa
    22261 cctggctttt cctaattcca caagtcgtcg aggccgtctg ggaaaacaaa gtgtgcatac
    22321 cttattaaca ggagaccaac aagcagtcgt cacggatcag tatcaaattc gaaagttaac
    22381 acctagggag tgctggcggc ttcagggctt tccggactgg gcttttgatc gagcttctca
    22441 agtcaattca gatagtcagt tgtataagca ggctggaaat tctgtaacag ttccggtcat
    22501 ttttgatatt gctagaagat taaaggaggt aaaaacagat gatttatgat gagttgattg
    22561 gggaatctac tgggtgattg gcaagattca gtcagatccg gaattggaag aagaactgta
    22621 ccggctcaat tttgatattc gcaaaaacgg ggtgaaagtc cctgatgatc cgtatgtaat
    22681 tgatgaggaa acggatgctc gaatggagct gaatcaagtc atcagtgaat tggaacgaat
    22741 tgcggacttt gctaaagaac ctgatatccg ccaatacttg tttgagataa aagcagagtt
    22801 ggaaattgag ggtattaccg cggagtaacg gccaaataaa ccactgatag gcactgaccg
    22861 cgggggattt gcgaagcaga aatcctccgt ggtgctgtgc ctgtttatgg acggcgttag
    22921 ccggccatga tgtctcaccc cccgtatcta acaggggggt acaaaaacac aaaaaataag
    22981 caagcaacga taaggaatgg ctgtcctatc aaggataata agcaatttgt gtagatgtta
    23041 actgatttga aaaagttaac accttaaatt tggaggtgag aacttggcac aaaggaattt
    23101 agattatcgg ctattaaaag accggcgtaa tgaatatggc gtttcacaaa ataaattagc
    23161 tacgacttgt ggacttagtc gcccgtactt aaatcagata gaaaatggtg gcgtaaccgc
    23221 atcgacaaaa accatgagga aaatttttga ccaactggaa agtttcaatc ccgagttacc
    23281 tttaacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg cgaggaaaat
    23341 cattcaagag attctccatt tgaaatttga ttatatgctc cacgaagatt acgcctttta
    23401 ctcctatcaa gaacaatatg ttatgggaga tattgtggtg atgttgtcac atgaagaaga
    23461 taaaggcgtc cttttagaat taaagggtcg tggttgtcgg cagtttgaaa cttttttact
    23521 cgcccaaaag cgtagctggt atgacttttt cgaagattgt ctaaaagccg gtggcgtgat
    23581 gaaacgctta gacctggcaa tcaatgatcg agttggcctg ttagatattc ctgatttaac
    23641 gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt accttcaaaa gctatcgttc
    23701 cggggaactg ttgaaacctg atgataaaga tggtatgggc aatactttgt atattggcag
    23761 tcttaagagt gaggtctatt tctgtttgta cgaaaaagac tatgagcaat atatcaagtt
    23821 agggattcct ttagaccaga ccgaaacgaa aaatcgtttt gaaatccgac tgaaaaatga
    23881 tcgagcttat catgcaattc aagatttgtt aaaaggtcgc agtattgaaa gtaccacatt
    23941 ttccattatt aaccggtact tgcgatttgc ggataaggtg gaaggcaaaa ggcgaactaa
    24001 ctggcccttg aatgaacaat gggggcgctt cattggacgg aatcagaagg aaattcaatt
    24061 aacttctgaa ccgaagcctt ataccattga acgaacgttg aactggcttg gacgacaagt
    24121 cgcccctacg tggaagatgg ccaaggagtt ggatcggttg aatcagacaa cgtacattca
    24181 ggatatggta aataatactc gactatcaga ccggcacaaa aagattttgg agcaacaaag
    24241 tatggcaatt gaaaatttga ttatatgaaa agaggaatcg tgatggaaat tgtgaaagat
    24301 attttattgg tgtttggtgg cagtatgctt ggtgttactg tgatgtgctt gatgcatgca
    24361 agtgcagcgg cggaccgagc aatggaaaag aaaggaaagt gatgaaatga atttcggtca
    24421 aaattt
//
LOCUS       DACCXM010000031        24418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCXM010000031 DACCXM010000000
VERSION     DACCXM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.033"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            360..1343
                     /locus_tag="IVY61_001072"
     CDS             360..1343
                     /locus_tag="IVY61_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP7970201.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            complement(1711..3849)
                     /locus_tag="IVY61_001073"
     CDS             complement(1711..3849)
                     /locus_tag="IVY61_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7970202.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(3888..5474)
                     /locus_tag="IVY61_001074"
     CDS             complement(3888..5474)
                     /locus_tag="IVY61_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7970203.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(5464..6684)
                     /locus_tag="IVY61_001075"
     CDS             complement(5464..6684)
                     /locus_tag="IVY61_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970204.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(6696..7502)
                     /locus_tag="IVY61_001076"
     CDS             complement(6696..7502)
                     /locus_tag="IVY61_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7970205.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(7712..8992)
                     /locus_tag="IVY61_001077"
     CDS             complement(7712..8992)
                     /locus_tag="IVY61_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7970206.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(9036..11069)
                     /locus_tag="IVY61_001078"
     CDS             complement(9036..11069)
                     /locus_tag="IVY61_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970207.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(11103..11954)
                     /gene="rfbD"
                     /locus_tag="IVY61_001079"
     CDS             complement(11103..11954)
                     /gene="rfbD"
                     /locus_tag="IVY61_001079"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP7970208.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(12052..13080)
                     /gene="rfbB"
                     /locus_tag="IVY61_001080"
     CDS             complement(12052..13080)
                     /gene="rfbB"
                     /locus_tag="IVY61_001080"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP7970209.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(13102..13674)
                     /gene="rfbC"
                     /locus_tag="IVY61_001081"
     CDS             complement(13102..13674)
                     /gene="rfbC"
                     /locus_tag="IVY61_001081"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP7970210.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(13688..14554)
                     /gene="rfbA"
                     /locus_tag="IVY61_001082"
     CDS             complement(13688..14554)
                     /gene="rfbA"
                     /locus_tag="IVY61_001082"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP7970211.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(14654..15388)
                     /locus_tag="IVY61_001083"
     CDS             complement(14654..15388)
                     /locus_tag="IVY61_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7970212.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            complement(15366..16193)
                     /locus_tag="IVY61_001084"
     CDS             complement(15366..16193)
                     /locus_tag="IVY61_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7970213.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(16193..16993)
                     /locus_tag="IVY61_001085"
     CDS             complement(16193..16993)
                     /locus_tag="IVY61_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7970214.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(16986..18125)
                     /locus_tag="IVY61_001086"
     CDS             complement(16986..18125)
                     /locus_tag="IVY61_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP7970215.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            18165..18323
                     /locus_tag="IVY61_001087"
                     /pseudo
     CDS             18165..18323
                     /locus_tag="IVY61_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(18330..19523)
                     /locus_tag="IVY61_001088"
     CDS             complement(18330..19523)
                     /locus_tag="IVY61_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7970216.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(19602..19808)
                     /locus_tag="IVY61_001089"
     CDS             complement(19602..19808)
                     /locus_tag="IVY61_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7970217.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(19801..20943)
                     /locus_tag="IVY61_001090"
     CDS             complement(19801..20943)
                     /locus_tag="IVY61_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7970218.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            21455..22090
                     /locus_tag="IVY61_001091"
     CDS             21455..22090
                     /locus_tag="IVY61_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7970219.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            22226..22318
                     /locus_tag="IVY61_001092"
                     /pseudo
     CDS             22226..22318
                     /locus_tag="IVY61_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            22432..23067
                     /locus_tag="IVY61_001093"
     CDS             22432..23067
                     /locus_tag="IVY61_001093"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7970220.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            23174..24412
                     /locus_tag="IVY61_001094"
     CDS             23174..24412
                     /locus_tag="IVY61_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7970221.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
BASE COUNT         7407 a         4930 c         4204 g         7877 t
ORIGIN      
        1 aaaataatat ctctattatc ttaccatagt cacctttaaa aacctagttt ataagcaaat
       61 tgtaatggaa tttaaaaatg tctaataaaa agctttatac acctctaatc aagacaagga
      121 ctctaaatta caaatctcca gatgaatcga ttttcactaa tgttggtaaa ttaaagaagt
      181 cgaagtattt gctgcttttt ttatccactc ataaagtgaa cctttatatt tttcagagaa
      241 tccatcttcc caaagcgctg tctagtccat aagaatacct gtattacgtg aagaatcgaa
      301 aaaattgaaa tcgattttta atgacattat gcttatagaa gaaagtgagg taatatctta
      361 tgaataaaca agagttgcat gattttattg gttttcgaca accaaatata tgtcaaattg
      421 tcgcatataa ggatggggta gaagtttata cagatgaatg gaatggttat aaaaaagacg
      481 atacttgtca tatcatgtca gcaacgaaaa gtattgtatc tttgctgatt ggcattgctt
      541 tagagcaagg attaatcaaa agtatcgatg ataaagtatt agattatttt ccagagtata
      601 aagtgaaacg cggggagaaa actatttacg ctattacaat caagcacttg ctaacaatga
      661 aagcacccta caaatgtaaa ggcgatccat ggacaaaagt ctgttcaagt gaagattgga
      721 caaaaacatc actagattta ttgggaggaa gaaaaggtat aaccggcgaa ttcaactatc
      781 aaactgtctg tttacacata ttaactggct tgttgtatca gacaagtaag ctaacaactg
      841 ttgattttgc caataagtat ctatttgatc ctatagggat accaaaccat gtcaattatt
      901 atgctgaaac agctgaagaa cataagcagt ttacaatgga taagtctcct aaaaccaatg
      961 tctggttttg tgatccacta aatatcggga cggcaggata tggtttgtgt ttatcggcaa
     1021 gtgatctggc aaaaattggt gtattatgcc taaataaagg gatgtataaa ggaaaacaaa
     1081 tcgtaccacc catgtggata aacgagatgt tcaaacctag aaccagtgct ttagaagact
     1141 tacaaaatat gagatatggc tatttatggt gggtattaga ttctgagaac gaaatatatt
     1201 cagcaattgg taatagtgga aatgttcttt atattgacgc taagaacaat ctggttatct
     1261 ccatcacttc ttattttaaa cccatgataa ttgatagagt ggcttttgta gaggaatacc
     1321 ttaaaccatt tattttaaaa tagcctatat taaaataaaa tatcgattct ttgacagcta
     1381 gggttcgtga aactttggct atgattttgt atctattggc gctctgagca tgaataagag
     1441 cgtgggacaa aagtaagaaa cacttttgtc tcattcttta aaaacgaata aacggtggga
     1501 gcagaagcaa cttcttctta aataagccga aattcacaaa aatttgaaaa gcaattttcg
     1561 tgaatttctt cttatttctc ggagttaaac acttctgtcc caacctcgac ttattttttt
     1621 gagtcggcac cttctgcctt tttttgacaa aaaactataa aaagccgtta tgtgcgagcg
     1681 aatcacaaca taacagcttt ttattatagt ttaatctggt ctcaacgaaa agtcgccgat
     1741 tccttctttt gttaaattat cattgtaaaa tggatcatgc gcaatatatg ctggccattt
     1801 atcttgcatt tttttgattt caccagcaaa acgtctttgt ttttctggag tatcttcata
     1861 acctcttgat ttagattcga aatggtacaa ttctgcttga tgtgcataga cattgtaacg
     1921 acccagttca taaactttca agcacaagtc aacatcgttg aatgctactt ctaatgtttc
     1981 atcaaacccg ttaacagcat tgaagtcagc tgccttgacc atcatacaag ctgcagtgac
     2041 tgctaagtaa ttgacgtcaa tgaccaatcg gccgaaataa ccacaatgtg ttcgatcata
     2101 gttattcaat gcatgccctg caacaccacc gattcctaat aatactcctg catgctgcgt
     2161 tgtatcatct ggataaaaca gttttgctcc tacacagccg attcgatcaa actgtgcata
     2221 agaaaccatc accgtcatcc aatctggctc aatcacttct gtatcgttat tcaagaataa
     2281 aaagtatttg ccgtttgctt tttctgctgc taaattgtta attcgagaat aattgaatgg
     2341 aatgtcaatc aattctacga taaagcggtc tttcagctga tgcttgtatt ctgcaaataa
     2401 ttcttgcatt tttggatctg tacttccgtt atccgcaata atgatttcat aatttgaata
     2461 agacgttttt tcgatgatcg aatccacaca agtttttaga tcttcatagc cattcttcgt
     2521 tggaatgatc acactgacca aatcctcttg caagacgtca taagcaatct cataaaatcc
     2581 agaaattctg ccaggacgta cgcttccttt gatgtttctt ctagacaggg catctgtcaa
     2641 tgctttcact ccggaatcat agatataact tttggcttcg ccattacttg cagttgatcc
     2701 tgggatcgtc cgccaatgat acaagactcg gtcaatgtgg taaatatgat ctgcagggat
     2761 ttgttctgtc acacgaagga ccaaatcata atcttgagaa ccttcatagc ctttacggaa
     2821 accgccaagt tctttaacaa tgcttgttcg atatacgcct aagtgagaga tataattatt
     2881 tcccatcaat gtatcaggcg accagtctgc tttgaaatgc ggatcaaagc gattgccatc
     2941 tgcatcgatt ttatcttcgt cactgtagat cacatccagt tctggatgta cgtttagcac
     3001 ttttgccact tcatataatg caaatggcgg taattcatca tcattatcta agagtgctat
     3061 aaattctcct tctgctattt ccaatgcaga attagttgcc agactgatgt gtccattttc
     3121 ttttcggaaa acgactttga ttctatcatc ttttgcttga taggattcca gacactttcg
     3181 gatagctgga tcagtagaag catcatcact gatacatagt tcccaatgat catacgtttg
     3241 atccagtacc gaatcaatgc atttttcgag ccatttgatt tcaacgttat agacaggcat
     3301 aagtatggaa atcaatggac ggtacgcaaa tccttgtgct tctttccttt gctctttcaa
     3361 atctggtttc tcatgacgac tgatccactc tgcatagtcg atcgatcctg gcttcaattc
     3421 caatttgacc cgacggatcg ctttttttac gccatgagtc cttgcataat ttatcccttt
     3481 tttcagtaat cttttttttc tttcccatga agattcaatg ccgtcatcat aagggtattt
     3541 accgtttaat ttcacagcta tgccattttg atgtctcgct gtttggaagt ctaagatggc
     3601 ttttcctctc attttccctg ataaacgtac cttaaaaccg ctttgggtct taacatcaag
     3661 attatataaa tgattgatat ccaatcttac gacatgtgtt gcttcagcaa tcacattttc
     3721 tttttctacc tttatcgtag ggatttcttt ggtcacttca tcaattgccc agcccacaat
     3781 aagcaaatcg ccagtggccc gttcacgagt gatttgatcg ataaatacgg caatttcttt
     3841 tttttgcacg attcgtttac tccttatctt tataagttaa tcattattta catttcaatg
     3901 atccatgatt ggccattttg agctactgct tccactaaga atgaacgttc ttgctcgcaa
     3961 aggattcgta actcaaatcc cgcttctgtt ccttttgcga gctgttctgc ttgattgact
     4021 tcttctcgtg aaacaggaat tgcttcgaaa aatggcgatt ggtcagctga taacttatag
     4081 gacaagggct ggcgatccga attagcgatt ccccatccga tgatttttaa tatttttgtt
     4141 tctgcatccc agattttctc gtcaatacat atagaaagtt ctttttcttg gatttttttc
     4201 gaagtttctt tcttacgtcg tcctagtcta tgttttattg tccattcatc actttccaac
     4261 aatgtgcaat agcgatttgt ggttgttttt aacgtatggt ctagtcttgt attttcctca
     4321 agaagatgat gattttctct ttctaattcg tcatttttct cagaaagctg ctttgtgact
     4381 tcagccatgg gacgaatcgt ttccaacaat tctttcattg ttggtgtcaa ttccccgata
     4441 aagcgatagt gacaatggat agtcacttct ttcccagaaa tatccgttag aacgaactct
     4501 ggtaattctt tcccatcaaa caaataacaa tcatttactg ttttgactac agcattcgaa
     4561 tcgataaatg ttagtttttc tccggcagct tctgcgctga attcgataaa gctaggttgt
     4621 tgcgcgaagc gaaacaccat tctttctgtt gtttctgata cggggaaact tagtgtctca
     4681 ttttcccctg tgaaattatt gaatggtatt tgttggattg tttcatcttg tttcgtttgc
     4741 tgggttactt ctaacacttt tacataatta gaattttgtg gctctgcaat ggaactttgt
     4801 gctactggat gtttcatcaa tgagaaaaag tattggtaga cttcaccaaa tggtcgcatt
     4861 ttgaaatcat agcgtacagc ttccggcaat tcttcataag ttgattttaa ttctgtatca
     4921 cctacagcta agtacaaata atcttcaata ttaatgaata atcccgcttt ttcaaaaact
     4981 ttttggaagc catcatgagt ataaaaagaa ttatgggttt catctaggag accataagaa
     5041 gcccacggca attggttatt gaacaaatcg atcatgacag aattatgcgc caagttaggg
     5101 aatgtaatca ggatctctcc ttcttcattc aaaaattctc tgactttctt caatgttttt
     5161 ccaggatcta ccaaatgttc taaaacatcc gcaaacaaga cgtaatcaaa tgtctgtcca
     5221 gcataatagt ttgcccactc aaaactttcg atatcaccat aaaatccatc ttgagcaaat
     5281 tcgctgacaa aatcgaacaa ttccttatct aactcaacaa tgctcacttc acattgtttt
     5341 gctccgatta aatgtttcgt taatcggccg tttccgggac caaactctaa aactttactt
     5401 cctggtttga tctgtgcggc aatttttccc acactgcttt gttcatctag gtccatttca
     5461 aaatcatatt tcattgctat gagtcccttc ttcccattta tggtctaaga ccacgatgcc
     5521 ttctccgata taagaaccgc tgacaaacaa gttcatgtac tttgtcttat ataccaaagg
     5581 aaccgttgca ttttcttcaa agaccgcaac gtcaaaataa tattcaccag ctaacaaacc
     5641 taacttttca tactccagat aaaagatatt ctctcctttt ttccaaggaa tcttgatacc
     5701 atctagtaga gtattaagtc cgcatacata aaaattgtcg actgttcgta gcgcaattcc
     5761 taaaaccgga tttttgattg tatcatcttt tacttcataa gttactttaa cgtatagttt
     5821 ttctccttgt tccagcatat ccaccggatt tttgtcttta tccaacaatt ccgctttcgt
     5881 cacgtcgacg atatctggag cggcgaaccg ttctacttgg tttttattcc gatccactgt
     5941 tttaactgat tttttcttta aaaagttttc ataattcgtc gttacttcca tcgtatcgcc
     6001 atattcgacg acttctccgt ttttcaacca ataggaacga tcacaaaaac gacgaatcga
     6061 actgatatca tgagaaacga ataaaatcgt ctttcccgag ttcttgattt ctgtaaattt
     6121 ttccatacat tttagttgaa attcaagatc gcctactgcc aatgcttcat ccacaatcaa
     6181 aatatcggga ttcacattga ttgctacagc aaaagctaga cgaacgaaca tcccgcttga
     6241 ataagttttt acaggttgat aaatatggtc tccgatgtca gcaaattcaa tgatatcatc
     6301 gaccatttca tcgacttctt ctcttgaaaa tcctagaacc attccattta aatagatatt
     6361 ttcatagcca gaatactcag gattgaatcc tgatcctagt tctagcaaag ctgagatttt
     6421 tccttcgatt tccatcgtgc cggatgttgg tgtcagtact cccgtaatga ttttcaacat
     6481 tgtagatttt cccgatccgt tttctcccac gaaaccgatc atctcacctt tttttatttc
     6541 tacattcacg tttttcaacg cataaaatac atcatgatag gatttcttaa aaggattgag
     6601 ggcttcacga aatcgatccg aaggttttgc gtacatatta taagattttg tgatatcttt
     6661 tagtctaatt gcatattctg tcatagctat tctccttaca gcacatctga aaaatgtggt
     6721 ttcaatctac ggaacactgc agagccgatt acgaacatta ctaacgtgaa aacccagaaa
     6781 tacaaagtat atgttccatg ctcccagaac caagcttcac ttaaaaatga ctcacgataa
     6841 ccttgaacaa catagtacag cggatttaat ttcaatatgc ttacaagacc tgaatcaaac
     6901 tgattagggc tccataatat cggtaatgtc cacatgatca actgcagcac gatcgtaatt
     6961 aattgcatga tatcaggtaa aaatggttgg atcgatgccg tcagccaagt caatcctgtc
     7021 aagaaagcac acatgcaaaa taaataataa atcagctgta aagacattgc tgaaggatat
     7081 ataccattta taagcgcgac agctatgcca atcagaatga agaataagtg ggtgtacaga
     7141 ttggatagga ttttcgtcgt gggcaagata cgaatattga atacgacttt ctttactaaa
     7201 taagtatatt cccgaaaaac aaccgtagtt gatgcaaatg catctgagaa gaagaaccag
     7261 ggaatcatcc cggtaactaa atatacgata aatgggtatt ttgcatcgat catttgtgct
     7321 ttcagtccaa caccaaatac cagccaataa atcaatacag taaatagcgg attgataaac
     7381 gcccataaga ttcctaaaaa cgatcctgca tatcttgatt tgaagtcatt gatcgaaaac
     7441 tgtagcaata attttctgtt ctgataaatg tccttcataa ataaatacaa atctttaaac
     7501 atttgtgttc ctccaagcga ttttcagtga attacagtca agtgccattt taccatattt
     7561 ttggtaccga gctgcgttct tatttctttc ttaattttct atgatacaag attttctcta
     7621 acgtttctat tttacagaag atgctagaca attgcaataa accaaaaaag gctgcgacgg
     7681 ttgtcacagc ctctctcgta ttttgtgcct atcaatgata gcgttggatc ctggaagaat
     7741 gagcgctatc tggttgttcc tcataagatg tatcatcata agtcatatcg tcatatgcct
     7801 cttccataac atagaattcc tccgcaactt ctggttcatc atatcgatga acggccatag
     7861 ccaacaggat ataaataatc ggaacccatg ggtatgaagt caatgtatcc tccataaatg
     7921 actgcatcat caccgcaaaa aaggcccaga taaaagtcat ctgcccgttg gataaatctg
     7981 ctttcaacgt taagcgacca ataaaaagga taagcagcaa agcagcaatc ccaaatccta
     8041 tcaatccata gatattcaaa atagaattat aactattgtg gtttcctaaa aattggtttc
     8101 ctcgttggaa gataaatgtt ttcattccta gtaaaatttg ttcagatttt tctcctagcg
     8161 tctgatagaa tttatgccaa atatcttctc gaccggtaaa caaattgact ttttcagaga
     8221 cagccgccag ataagaaatc ggcaaagcta aaagaatcgt aaccgtgtaa gcaataaatg
     8281 gcgcacgaat gacacgcatc aacgatttag gtaaaagatc caatatcgca aaaacaatca
     8341 acgcgactaa tgcacctcta gattggcaga cccaaatccc gagaaaagct gcaacaactg
     8401 ctactgcacc gatgattcgt acataccatc gttcaaaaga actggcaaat ccactgatca
     8461 ataaagcaag agtcatcaaa gaacttccga ttatattcgt attgatccaa atatcagaaa
     8521 gcgaattgcc tggagcccag atgctttgtc cttctggtac ttggatctct gtaaatacac
     8581 gatataacgt gattccgagg aagaccgaaa agacaatcag ataaataccg cgatcccatt
     8641 ttgtccactc gatccattta attactaaac aaaataaaat aatcaaagga aacagtaagt
     8701 ctccaaataa agactcattt cttaattccc gaaagtaaga aaagaaaatg aaaagggcaa
     8761 gcgcaacacc tagataaaga aaatcgacgc cttttgcttt ataaacattt aaaatgagca
     8821 aaatagccac actaaaagcg ataagtaatg aactattgtt atatataaac aaagttgctg
     8881 gaaaacctaa ccctgctgtc caaacaaaaa agactagcgt cagtaacaaa agcaagaaac
     8941 cgacactcat tttgttgaca aatttttgta cgattgcttg aattatctcc actctccttt
     9001 ctactaacat ttcaggatta ttctcagcta gcctattact gtacgactag ctgattttcc
     9061 tgagtttgtg tttgtggtac atctggataa tccggatatt ctactttaaa aatcatataa
     9121 ccgtctttat ccggtccata tagctgcgtg aactgcgctt catataattg gttgtaatca
     9181 gttaatggtc gtttactgat cacatagtcg atccctaatt tttctgcgtc ttcaaaattc
     9241 aacgtgaccg aaaacatatc tggacgatct aaaatgaact ttgtatcttc cttagcgata
     9301 aaagctcttg tatgagcata ccgattataa tacttctcgt attcttcttt cggatcgatt
     9361 ttcttccatg cagctatatc tggataaaag cgtactgtat tgaaggattt cactcccaat
     9421 gttggggtga agttatataa ttcatcttca gacaaccaaa ctttaccagg atcttgttga
     9481 tcgatttttt ggatttcttt tgccagtgtt ttttcaaaaa tcgctcctgt accttctacg
     9541 actggattga cgaaaaagcc agatacgata actaaggcgg caaaagcgag gtaaaacaag
     9601 cgtctccagc caaataatgc aaaagctaaa atcactgttc ccaacacagc aactgcgatg
     9661 gtttcaaaat ccgaaaaata attatccatt ttcgaatgcg taatcgcata gaaataagac
     9721 catgcattta ctgccaacaa aatgaattta taccaaatag gaataacatc ttctctccaa
     9781 atataagcga taaaccagat cgataataaa caagccgcaa aactaaaagt aaggatggct
     9841 cttaatggag gaacataaga cagcagagtg atttctgcta gccattttgg caacccaacg
     9901 gtaatccaaa agatagaaaa cagacaaaaa atcatcaaaa tacgtcccag taacttttgt
     9961 tcgctatctt ttttgccaaa taacacaaat ggacttgcta ggaatacagt tggaaaaaag
    10021 tgatagaaca gggccacttc agagttattc gaatagttga tatcttcaaa aggaattttc
    10081 caatttgtaa ggaaagaaaa aaattcgcca atcgtccact ttcctccagt acttacccgt
    10141 tttcctggat ataaggtatt caaagaagct aacaatgcgt cttttgacgt tagccagaaa
    10201 tgaaccatga ctccgccaat aaataaaact gcccctccaa tcaacactac atccaacaga
    10261 tcccatgaaa ctttcttgct atagtaaata agcaggccaa caaaataaaa aatcagcaag
    10321 tacgccagca tcacttgatg ggctgggtaa atcactaaaa taaacccaat accgaaaata
    10381 accaccaaaa gcatcatcaa cgttcgtagc cattttttat catgattgta gaaataatga
    10441 taaaaagcag tcatcagtcc caaagtaaag aaaatcaaat cgcccacatg ttgcatgaac
    10501 caccactgga ctgctggaga aaatgtcagc gcaaatgcac cgattagcga tagagctttt
    10561 tttctcttcg ttaatatcat cagtacttcg aatccaagca agaccatgac gactaatttc
    10621 atcgcccaat aaaaggaaag tcctcgatct cttccgagga aaaagaatcc ccagttgaat
    10681 ggttttccga taaccgtaat atcctttaca ggagaattat acgcaatgat catgttttgt
    10741 ccgtttaatg aataatcctc gttcaccaac ggaaagtcct cttcagcttg tgagaggtaa
    10801 aaaggtgtct gcaccagcca ttcgtctgac cgcacttccc gattctgtcc aaaaatcaca
    10861 tcagtttttt tcccatcgtc tctttgcgac acatatttat cccaaacacc aagtgaactt
    10921 ccgtttaaat taaaagtaac gatgaccacg atgactaaaa cgccaataag atagcggagt
    10981 tttagaaaca ggtcaagcac caaattcgaa tctgcctggt tcgcttttgg cttttctttt
    11041 cgtcgtgcac ttcttgtttg tttttccata tcatgctcca aattctattt gataaaagga
    11101 aattacttat tcattttttt ggcagcttcc agcatttgcg caagtgcttc ttgccaagtt
    11161 ggtatgacaa atcctaactc ttctgtcttt ttcaggctca tgactgaata ctgcggacgt
    11221 gttgcttttt gcggaaattc ttctgacgta acaggtgcaa cttctacatc tgtatctttc
    11281 aatatttctt ttgcgaattc ataccaagaa cagctatttt cgttcgacaa atgataaata
    11341 ccaaatggtg ctttttcttc gatgacatat accataaatt ctgccaaagt acgtgtccaa
    11401 gttggtcgcc caaactggtc atttactaca gtcagtttgt cgcgggtttc cgctaggcgt
    11461 tgcatcgtga agacgaaatt gtgtccatag atcccgaaaa cccaagaagt tcgtatgata
    11521 taatagtctt ctaaaatctc ttctactgct ttttcgccta ataatttcgt acgaccatat
    11581 tcatttagtg gattcgtttg gtcgtctact tcgtattcta catttttgtt ttttccatcg
    11641 aaaacatagt ctgtactgat atagacgaat tgagcattca cagcttttgc tgcaagtgct
    11701 acatttcttg taccatcaac attgatcttc tcatctaatt cttttgcttc ttcttctgct
    11761 ttatctactg ctgtgtaagc tgcacaatgg aaaataacat caggttttaa ttctgtgatg
    11821 taagccattg ttttctcggc atctgtgata tccaagtctt ttgcatctgt tgaaacatat
    11881 tgcagatttt tttcatctaa taaatggcgc aattctgtac ctagttgccc atttcctcca
    11941 gttaataaaa acatatgatt atccgctcct tgatttttat tttaaaaaag agacaggcga
    12001 gcgcctgcct cacatgtgtt tttccattaa tacagtgaca ttttctctgt attattgtcc
    12061 atttttggca tatgctgctt caacggcctc tttttctttt ttccaccagt cttcatgttc
    12121 ttcgtaccat ttgatcgtgt cagccaaccc atcacggaag ttcgtaaact caggcttcca
    12181 tcctaactct tcccgtaatt ttgtcgaatc gattgcataa cgcaagtcgt gtcctgcacg
    12241 gtcgtttaca tggtcgtacg catcttttgg ttgacccatc atttctaaga tcaattccat
    12301 cacggtctta ttatcttctt caccgtctgc accgatcaga taggtttcgc caattctgcc
    12361 tttcgtcaaa atcagccaca ctgctgaaga atgatcattc gtatggatcc agtcacgaac
    12421 attctttcct tcgccatata attttggacg aataccgctt aagatattcg ttacttggcg
    12481 aggaataaat ttttcaatat gctgataagg accatagttg tttgaacagt ttgaaatggt
    12541 tgcacgtaag ccaaaagaac gcacccatgc ttttactaac agatctgaac cagcttttgt
    12601 agaagaatat ggacttgaag gattatatgg tgtttccgca gtgaattttt ctccttcgcc
    12661 ttccccatgt cctggcaggt cttcacgtaa tggaagatcg ccgtaaacct cgtcagtaga
    12721 tacgtgatga tagcgtacat catacttgcg gcaagcttct aaaagtgtat acgtaccaat
    12781 gatattcgtt tgtacaaatg ggaaaggatc ttttaatgaa ttatcgttat gggattctgc
    12841 cgcgtaatga acaactgcgt ccgcttcttt tactagacgg tccactaatt ctgcatctgc
    12901 aacatctcct acgaccaatt ctacgcgatc gctaggaagt ccagccaagt tctctttgtt
    12961 tcctgcataa gtcagtttat ccaaaacagt gacatgcact tctggatgat tgtttaccac
    13021 gtaatgaacg aaattagagc cgatgaatcc ggcacctccg gtaacaatga tgtttttcat
    13081 aagaaaattt ctccttaatt cttagatttc tccgtaaaca aatggatttt ctgcttcaaa
    13141 ttcttttaat gtcggatgac ggtgatcttt ttccgacata atcgctcttg atggatcaat
    13201 tggccaatca atcgctaaat ctggatcatc aaatgcgatg cccccatctg cttctgcatt
    13261 atagtaatta tcacacttat aaaggaagtt cacatttggt gtcaaagtaa cgaatccatg
    13321 ggcatatcct ttaggtacaa gcaactgtcg atgattatgt tcagataaga taaatccgtc
    13381 ccattgacca taagtcgggc tacctttgcg gatatccact actacatcta aaactgctcc
    13441 agtgactacc cgaatcaatt ttgtctgtgc agcttctcct ttttggaaat gaagcccccg
    13501 aaggacacca gcttcagctg atagtgaatg gttgtcttga acaaagtcat atttcaatcc
    13561 atgttctgca aattttgctt ttgaataact ttctgtgaag aaaccgcgat gatcgccaaa
    13621 gacgtccatt tctacgattt tgacatctgt taattttgta tctgttactt tcatctgtaa
    13681 ggactcctta ttcagcggcc aaattcaata aatattgacc gtattgattt tttttcaatg
    13741 gctgtgcaag ggccagcaat tgttccttat cgatgtagcc catacggtaa gcaatttctt
    13801 ccaaacaagc cacttttaag ttctgacgtt tttcaattgt ttcgataaag gtagatgctt
    13861 caagcaatga ttcatgtgtt cctgtatcaa gccaagcaaa tccgcgtccc ataagttcta
    13921 cggatagttt tcctttatcc aaatatgctt tattgacatc cgtaatctct agttctccac
    13981 gttctgaagg tttgatacct ttagcgatag aaattacctc attgtcataa aaataaaggc
    14041 cagttacagc gtaatttgat tttggctgag caggcttttc ttcaatcgac aaagcttgca
    14101 tattctcatc aaattctact acaccaaagc gttctggatc attgacatga tagccaaata
    14161 ctgtcgcgcc tgtatctttt gaagctgctc gttgcaacat tttagacaga ccgccaccat
    14221 agtaaatatt gtcccctaat accaagcaga cactatcatc tccaataaat tcttctccaa
    14281 tgataaacgc ttgtgccaat ccatctggac tttcttgtac tgcatattcg atctgcagtc
    14341 ctaaatcatt tccgtttcca aataattcct tgaaacgtgg tgtatcttgt ggcgttgaaa
    14401 taatcagaat ttctttgatt cccgccaaca tcagagtaga cattggataa taaatcattg
    14461 gtttgtcata aatcggcatc aattgtttcg atgttgcctt tgttaaaggg tacagacgtg
    14521 ttccgctgcc cccagcaaga atgattccct tcatggatat ctgctctcct ttttgatttc
    14581 aaatttcact ttgttattat ctcatcaatt gttatatttg tcatggaaat attaaggatt
    14641 taaacatctt ttatcaacgt ttaaaccatt ttcgtttgat caacgccgta atcagatgaa
    14701 cacgccaaga aattttttgc cgtaatttag aatgtgtttc aatctcactc gcgtttaaat
    14761 catgtcttcg gtacagagtt aacggttctc ttaggaaccc agtcttcttt cctcccagca
    14821 aaccaatcca catatcatgc atcggcactt ctgacgggat cggcaaaata tcgggtttta
    14881 aagaagaccg gaaagccata ccagcaccaa tatagtgact tcttatcaca ttattaagcc
    14941 atccttcttt ggaatttcga aacgaaaaat acgaatcatg gatctcttgc agctcgctgt
    15001 ccacaatgat taaatcagaa actactacta gctgatccgg attctgctca aaatagtcag
    15061 tgatcttttt cacttttgtc tccaaccaaa catcatcttg gtctgccaaa aagattagat
    15121 ctcctttcgt ctgctggatc gcataaccaa aattagcgat caatccttga cgaggccccg
    15181 aaaaaagctg gattcgctta tctttttctg cgtattcagt taatatatcc catgtatcat
    15241 ctactgaccc atcatcagaa atgactactt catcttctgc tgaaagctga gataaaatac
    15301 tatctaactg ctctcttaaa tatctgcctc cattaaaggt agctatacaa accgaacgca
    15361 cgaattcaca ccttccaatt cttaacatat gagcgcaaag tcatccgcca gatatgccgg
    15421 tttttgacgg tcaagaactg tttagctgtt cttaacaata attgtcgtcg gtgactggat
    15481 aaatgccttt gatcatattc atgataaaat cgattttctg cttgtaaaat agaacggtat
    15541 cgattacttg aaactttttc atagtctaaa accgaaagat catgttccat tcgtacagga
    15601 aggatctcca cagttttttc caactgatcc actttccaaa acagccaatg atccaaaaaa
    15661 tctaaagaaa aagcaggatt aaacccgcca atctttttta gaaatttgac agaccaggca
    15721 acacctgaat tgattgccat gattcgttca gctgttgggc cttcaacttc tactgcttga
    15781 gctgtacgat tgatataacg atctgaatat aggggagaaa cttgtcggct cccagaaaaa
    15841 atcatcggaa ctgcagcaac acattcatct gtaaaggtta ctttacgcaa tagttcaaaa
    15901 taatcgtcga ctagcctcgt atcctgatcc agagtgacga gacaacgtgt tccttcatct
    15961 gcttgagaca acgcaaaatt ataagccacc gctaaaccag gattttgcgg atcatgatga
    16021 taaacgatat tttctttttc aaacagctca ttttgctgcc tttgaggact attatcgtaa
    16081 ataataagct gaatttgctt ttcgctaagt gctttgctta atggtccata agctggagat
    16141 tgactgaata gttgctgata aagcacaacg actactaata tttctggttt catcagaaaa
    16201 aacgccaccc ccacttattg aaaaatttca tcatagaagt caaaaacacg cgaaacagtt
    16261 ttgcatttcg atgcgcaccc ttcccgtaaa aatggataac tgcataaaag ggtgtgtaca
    16321 ataacttgta acctgctttt tcaaaactca aacacaagtc gttgtcttca aagtacatga
    16381 agaaacgctc atcaaacccc tttatctctt taaatttatc aatatcagct accataaaac
    16441 tacctgagat catccgtaca taactattag catcagtagg caatcctcgg cattcaaaca
    16501 cagccagtcg tttgtcaaac ttttttttga cccatttgaa aggcacaaag cgcaaaacgt
    16561 aatcaaaaac agccagctgc tcacggatga ggtgctgagt tgttccatct tcattaagaa
    16621 tcttaggtgc taacatcgca cattccggat gttctttcaa taacaagacc aatgctgtaa
    16681 ctgtctcttc atccactaaa atatctgggt tacaaataat gccatagcga gaatcgctag
    16741 ctatcagatt gtgattatgt ccataaccaa atccggaatt ttcatcatga aaatagatat
    16801 cgatgtaggg atatttccta tactctgtta gtcttttttt gtactcggga agtgaattgt
    16861 tgtcaaaaat tcggattttt actgctgttt catcttttac agttgaaata aaattatcta
    16921 gtgtccgaaa aattttatca ctgtttgccg tcacaatact aataaggatc ggctggttta
    16981 atttgttatt catctatatg acggttccat ttcttcaaat atttctgttt tacttgcgta
    17041 cggtactctt tattccctaa aaatcgcaat gtttttaaaa taaactgatg tttctcattg
    17101 atcagaccaa tcaattcaaa taataaaatt gcagcaaaca acatggcaat aatcaatatg
    17161 atcgttccga ttcttccagt ataattcatg accatagctg tgaaagaaaa catcaaagcc
    17221 atcgcataaa tcgtcaggac agcaccttta tgagtaaagc ctaaactgat tagtcggtga
    17281 tgaagatgtt ttttatctgc tgtagaaaaa gaaacacggt ttgcttttct cctaatgata
    17341 gcaaaaaacg tgtctgttac cggcactccc atgacgatca gcaaagaaat cgatgaaaca
    17401 aaagtcacgt tcttcagccc ttgtagtgaa aggacggcca tcataaaccc taggaataga
    17461 gctcctgtat cgcctaaata aatctttgcc ggataaaaat tgtaaggaaa aaaaccgata
    17521 atacttgcta caagacagaa aataacaact ggaatataca ctgtagaagc atggaggaaa
    17581 aaatatccaa tgattcctat cgtaattaaa ccgatcaatg aaatccctgc cgccagccca
    17641 tccaaaccgt caatcagatt gaccgcattc gtgataccga agatccaaaa aatcgttacg
    17701 ggaaaactaa gccagcctag ttggatatat ccaataaaag gaagcgtgaa ggaatcaata
    17761 cgtatatctg ctacaaaata aataataaga gaagcaatca agatccctat acttttttgt
    17821 ttaggtgtca gctcatattt atcatcgagc actcctgtga ctatgattac taaagaagca
    17881 atcaagatcg gtacaatata cgattgaggg ataattttac ggaataagaa taaagtcgat
    17941 ataacaaatg aaataaagat aggcagtccg cccgctgaag gcattggaac tttatttatt
    18001 cttctttcat ttggtttgtc gaccatacct gtttgtactg aaatgatttt gaatacaggt
    18061 gttaatatca gtgataataa aattgtcaga aaaaatctta taaccatact aaaagaaaca
    18121 agcatatggg cacctacttt aattaagagt cataattatt ataaattata tatgtctttt
    18181 tgactatctt tttatatctt ctcaaatgtc cgcaaagcct tattttatcg gctctacggg
    18241 cattttgctt ttgtggtaaa cctcgcatat ctaggtctat cttcttatat tttccctgtc
    18301 aatcgtggtt aaaatcgtgg taaatacttc tatgcgatta gacgctgaac ctctgatttt
    18361 gcggtatcta tggacgcatg agcgtaccag ttcattgtga tactgatgtt tgaatgcccc
    18421 atgatatact gtaaatcttt cgggttcatg ttcttgcttg ccagccttgt gcagaatgta
    18481 tggcgtagcg tatgcggtgt aatatgtggc aagggattgt ccttgtggtg cttgttgtat
    18541 ttctttacca tacggataaa taaggcgtta taatcaatgg caacttttgg attacctttc
    18601 ggattgataa acacaaaacc gctgtgtccg tctacctcta tcgactttat ctttggtcgg
    18661 ctgttgataa cccgttgaaa tgcctgtatc gtttctttgc ttaatggcac ttgccttatc
    18721 ccgcttttcg tcttaggcgt ttcaatataa tagccctgtt ccttactctt tagtaactgg
    18781 tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag tccgcacagt
    18841 tccgagatac gaagtcctgt ctttaacagt atcagcacat catcataatg cttgtgatac
    18901 acgttgtccg tcttaataaa tgacaataag gcttgctcct gttcctctgt caatgcgact
    18961 ttctctttgg tatcattttc tatgacttca cttagcttga aatcaaaagg gtttttcctc
    19021 acataatcat cttgtatggc tatgtagaat gacgctttta acgagcgttt atggttgtta
    19081 atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcttt agcgtcagag
    19141 ggttttatcg tatcaatgct cctagcacct aacttgtcct cttttaataa ccgcatgagt
    19201 tgttcccgtt gtttctgtgt gcttttcttc acatttgtcc tctgtgcgtt ctgtttggca
    19261 tagagttggc aaagcgtcat tttcttgcct gtgctgtcga taccgtcctc aatatcccgt
    19321 cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt tggtgtcggg
    19381 tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttcccaatgc gtctacatat
    19441 ttatataagt attttccgtc tgttcgttgg ctctctccag tatgcaggat acgacctttg
    19501 ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa gagccttgat
    19561 atgacttgtg ttcatcataa cacatacaag gctcatttgc attagattgc gtccaattta
    19621 tcaataacct tttcaaactg tcggcgttta atctgtatgc ggttgccatt cataatgagc
    19681 cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc aatgcggaaa
    19741 tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat atcctgtgta
    19801 ttactcataa aacaccccct cactttcgat tacgtctgat atgtccgtac acatctgttc
    19861 cacaagcatt tcctgttttt cagaaaggtg ggtacattca tcaatcattt tcatgtagtt
    19921 tgaacctgtc agcctgtcga tttcctctat catcacgatt gacggcatga cctgtctttc
    19981 cagccattgc agagtgcgtt ccatattgac aggtacaggg ttcatttcaa aaggtaaact
    20041 gtcatgggaa atgaatatct cccacaatgg atagtcagga aattccacaa aatcggtaat
    20101 gagataaaac accagcccat gagggttata cgtcaataac agttcttcaa ctgcctgtac
    20161 cgcctttttg tcacgcagac gaatttcaaa acggttgata atatcggtat gtttcttttt
    20221 cgttgcctgt tccttttgtt tttcatacag gcagaaatat tttgtgcttg cctttgagcc
    20281 gatataaagg gtacttgcta aattacggtt ttttccgctt aattttccac cttgtacatt
    20341 ttcatagttt ttacagcgac attccgcacc accatttctg tatttttcag atagcacagg
    20401 aatatccagc agaccgcaca tatcattgat tgccaagtca aaacgtcgga taacaccgcc
    20461 acagtcaaga cagcggttta agaatgaata ccagtccctg ttctgtgcct gtaagacata
    20521 ttccatgttg cgtgagcctg ccccttttaa ttctaaaaat acgcccatgt gttcatcatc
    20581 agaagccatg accttgattt ccccgtaagc ataatgctcc ttatagccat aatacccata
    20641 gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa cttccaatgc
    20701 gtcagtcgtt ggaaaacgga cagacagata atcaacgagc agataaaaag gcttatcgca
    20761 gatatagtaa ttcagacaac gcaggataat gttctgtgtt tccttgctgg cgttagcttt
    20821 tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg caagttctcc
    20881 ttgtgaaaga ccgctggcaa gacgtttgtt cttcatttca agtgcaaatt ctaaattgtt
    20941 cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaaccacc tttcaaaaag
    21001 tgtgatatgt actatcttct taaaacctct cattactact cacaggtttg ggggtgtatg
    21061 gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg gcacagacaa
    21121 ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg tgtcatggcg
    21181 gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag ttctgttttg
    21241 ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa tcagataggt
    21301 atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta caagaacata
    21361 gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc atattaagca
    21421 tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa tgaaaataag
    21481 ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac aacaggaact
    21541 tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag ggttgaacta
    21601 tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc ccaatgaatt
    21661 gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc agtttttaag
    21721 agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact tctttgacag
    21781 cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta cagactatat
    21841 caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta tccagcattt
    21901 gaagtttcaa aaattattag accgctatga tgatttatac agtatggata tgtttgggga
    21961 aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga taaacccgaa
    22021 ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt ttgatgatga
    22081 ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc cgatcaagag
    22141 tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt ctgttgtggg
    22201 caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa aggcaacgca
    22261 ggtatcacag tatgaattga caccgcataa ggaaagcaac tggaagattg tagaataaat
    22321 gaaaaaggct tgcattctgc atttttataa aattgcaagt ctttatattg acaagcataa
    22381 aatactaaga tatactttaa tcagaccaac ggtttgtatt ggaggtaagt tatggcacga
    22441 aataaatatc cagaagaaac agtaaaactc attttagatg tttctacaaa aatgttttgt
    22501 gaaaaaggat atgataacac atcattgcaa gatattatca atgaaacaaa actttctaaa
    22561 ggtgctattt atcatcattt cgattcaaag gaagatattt taaaagcaat ttttcaaaga
    22621 attggaaatg aaaatgcagt aatatttgct aaaattagag ataacgatag tttaaacggt
    22681 ttagaaaagt taagagaaat atttaagaca gcggtattca attctaacca aagcgtttta
    22741 ttgacagttt ctcctcaact attaaacaat cctcgttttc ttgctatgca aatagaacaa
    22801 atgtatgaaa ttgtagtacc tgaatttgtt gaacccgttt tagaacaagg tatcaaggat
    22861 aagtctttgg agattgaaaa ttcacatgaa attgcagaag ttatcatggt attaagtaat
    22921 gtatggctca acccattagt tgaaatgacg gataaagagg gtatggaaaa acgttgcaat
    22981 acattcaatg cccttttaaa tggaataggc atagacacat tattagacga agaaacaatt
    23041 tctgcattta tacagttttg taaataactg tatttgttat ccctatacga tagactgctc
    23101 tataaaagag cagtttattt aaaccattat ataaaccgac ggtctgtttg attaaataaa
    23161 aaggagagat tttatgcaag cagaaaaaaa actttttaat cgtgatttta tacttgttgt
    23221 tataggacag ataatttctt tatttggaaa cgctatccta cgatttgcat taccgtacta
    23281 tttgttaaaa gaaactggtt catcagcttt gtttggtatt gctacagcct gctctttttt
    23341 acctatggtt attttgtcgc tggtaggtgg tattattgct gacaggacaa ataaaagaaa
    23401 cattatggtc atactagatt ttgtaacagc aatcataatt ttagtatttt atttgtcatt
    23461 gggaaaaatt cctatcatac ctttgtttat tgttgctttg atgttacttt atggtatttc
    23521 tggtgcatat caaccagcgg tacaagcaag tattcctcta ttggtatcat cagataaact
    23581 taccgtcggt aatgctataa taaatcaagt aaatacttta gcaaacttta tcggaccagc
    23641 tataagcgga gtagtttttc aattttatgg tatcaatccc attttaattg ttagtattat
    23701 atgttttact ttttcagcca ttatggaaat ttttatccat attccacacc aaaaaagacc
    23761 tcgtgaagta ggtgtaattg cagttgctaa aaacgattta aaagatagtt ataccttttt
    23821 gaaaaaggag aaacccattt ttatatccgt aatctttttg gtttgcattt tcaatttggt
    23881 tttaagtgct gttttaattg ttggtctgcc tatcttaatt tctgaagttt taaaaatgcc
    23941 agatacacta tatggttttt cacaatcagc attatcatta ggtggtttat gtggtggtat
    24001 tttaacagct cttgttgctt caaagttaaa actaaaaaac ctttatacct tgttacttat
    24061 ttgttctgtt tctgttttac ttatgggaat ttcgctattt ttagaattgc ctgcaatagt
    24121 ttcctactgg gtagttacag tgatgtgctt tgtggctatg ggtgtatcta ctatgtttgt
    24181 tgttcaaatt tacactcttg ttcagacaca aacaccaccg caattagtag gaaagataat
    24241 ggctacgctg gtagcagttg ctatgtgcgg tcaacctatt ggacaaatgg tttacggtat
    24301 tttattcgat gtatttatac aagaaacatg ggttgttatg tttgtagctt ctattgcttc
    24361 aatcatgatt tcaatatatt caaaaagaat ttttcaaaag ttagagatat aatacaag
//
LOCUS       DACCXM010000032        24174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCXM010000032 DACCXM010000000
VERSION     DACCXM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.034"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            133..3660
                     /locus_tag="IVY61_001095"
     CDS             133..3660
                     /locus_tag="IVY61_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7970222.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IVY61_001096"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IVY61_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP7970223.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IVY61_001097"
     CDS             7448..7789
                     /locus_tag="IVY61_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7970224.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IVY61_001098"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IVY61_001098"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP7970225.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IVY61_001099"
     CDS             9146..11566
                     /locus_tag="IVY61_001099"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP7970226.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVY61_001100"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVY61_001100"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP7970227.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IVY61_001101"
     CDS             13000..13737
                     /locus_tag="IVY61_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7970228.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IVY61_001102"
     CDS             13751..14566
                     /locus_tag="IVY61_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7970229.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IVY61_001103"
     CDS             14579..15226
                     /locus_tag="IVY61_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7970230.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IVY61_001104"
     CDS             15242..15898
                     /locus_tag="IVY61_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7970231.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IVY61_001105"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IVY61_001105"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP7970232.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IVY61_001106"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IVY61_001106"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP7970233.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IVY61_001107"
     CDS             18240..18758
                     /locus_tag="IVY61_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7970234.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IVY61_001108"
     CDS             18865..19365
                     /locus_tag="IVY61_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7970235.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IVY61_001109"
     CDS             complement(19645..20565)
                     /locus_tag="IVY61_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP7970236.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IVY61_001110"
     CDS             20730..21743
                     /locus_tag="IVY61_001110"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP7970237.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IVY61_001111"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IVY61_001111"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP7970238.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="IVY61_001112"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="IVY61_001112"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7970239.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="IVY61_001113"
     CDS             complement(23383..24144)
                     /locus_tag="IVY61_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7970240.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
BASE COUNT         7993 a         4222 c         5107 g         6852 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgtt
//
LOCUS       DACCXM010000033        24117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCXM010000033 DACCXM010000000
VERSION     DACCXM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.035"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="IVY61_001114"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="IVY61_001114"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP7970241.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2485)
                     /gene="purK"
                     /locus_tag="IVY61_001115"
     CDS             complement(1352..2485)
                     /gene="purK"
                     /locus_tag="IVY61_001115"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP7970242.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="IVY61_001116"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="IVY61_001116"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP7970243.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="IVY61_001117"
     CDS             complement(3373..4680)
                     /locus_tag="IVY61_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP7970244.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="IVY61_001118"
     CDS             complement(4683..5264)
                     /locus_tag="IVY61_001118"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP7970245.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="IVY61_001119"
     CDS             complement(5836..7128)
                     /locus_tag="IVY61_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7970246.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="IVY61_001120"
     CDS             complement(7125..7748)
                     /locus_tag="IVY61_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970247.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="IVY61_001121"
     CDS             complement(7751..8587)
                     /locus_tag="IVY61_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7970248.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="IVY61_001122"
     CDS             complement(8604..9491)
                     /locus_tag="IVY61_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7970249.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="IVY61_001123"
     CDS             complement(9507..10790)
                     /locus_tag="IVY61_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7970250.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="IVY61_001124"
     CDS             complement(11473..12156)
                     /locus_tag="IVY61_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7970251.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="IVY61_001125"
     CDS             complement(12174..12791)
                     /locus_tag="IVY61_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP7970252.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="IVY61_001126"
     CDS             complement(12801..13625)
                     /locus_tag="IVY61_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP7970253.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="IVY61_001127"
     CDS             complement(13618..14178)
                     /locus_tag="IVY61_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970254.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="IVY61_001128"
     CDS             14352..15308
                     /locus_tag="IVY61_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7970255.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="IVY61_001129"
     CDS             complement(15613..16569)
                     /locus_tag="IVY61_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP7970256.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="IVY61_001130"
     CDS             16673..17275
                     /locus_tag="IVY61_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7970257.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="IVY61_001131"
     CDS             complement(17363..18862)
                     /locus_tag="IVY61_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP7970258.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="IVY61_001132"
     CDS             complement(18878..19402)
                     /locus_tag="IVY61_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970259.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="IVY61_001133"
     CDS             19700..20449
                     /locus_tag="IVY61_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP7970260.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="IVY61_001134"
     CDS             20440..21225
                     /locus_tag="IVY61_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7970261.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="IVY61_001135"
     CDS             21194..21910
                     /locus_tag="IVY61_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7970262.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="IVY61_001136"
     CDS             complement(22012..23031)
                     /locus_tag="IVY61_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP7970263.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24108)
                     /locus_tag="IVY61_001137"
     CDS             complement(23050..24108)
                     /locus_tag="IVY61_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP7970264.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7136 a         5207 c         4212 g         7562 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcct
//
LOCUS       DACCXM010000034        23828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCXM010000034 DACCXM010000000
VERSION     DACCXM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.036"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(53..787)
                     /locus_tag="IVY61_001138"
     CDS             complement(53..787)
                     /locus_tag="IVY61_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7970265.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IVY61_001139"
     CDS             1114..1524
                     /locus_tag="IVY61_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP7970266.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IVY61_001140"
     CDS             1517..2218
                     /locus_tag="IVY61_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP7970267.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IVY61_001141"
     CDS             2218..3804
                     /locus_tag="IVY61_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7970268.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IVY61_001142"
     CDS             3823..4044
                     /locus_tag="IVY61_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP7970269.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IVY61_001143"
     CDS             4041..4802
                     /locus_tag="IVY61_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP7970270.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IVY61_001144"
     CDS             5182..5691
                     /locus_tag="IVY61_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7970271.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IVY61_001145"
     CDS             complement(5766..6305)
                     /locus_tag="IVY61_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP7970272.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVY61_001146"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVY61_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP7970273.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IVY61_001147"
     CDS             7372..8169
                     /locus_tag="IVY61_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP7970274.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IVY61_001148"
     CDS             8197..8832
                     /locus_tag="IVY61_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP7970275.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IVY61_001149"
     CDS             8852..9625
                     /locus_tag="IVY61_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7970276.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IVY61_001150"
     CDS             10033..10830
                     /locus_tag="IVY61_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP7970277.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IVY61_001151"
     CDS             10808..11221
                     /locus_tag="IVY61_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970278.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IVY61_001152"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IVY61_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7970279.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IVY61_001153"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IVY61_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP7970280.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IVY61_001154"
     CDS             15998..16558
                     /locus_tag="IVY61_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP7970281.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVY61_001155"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVY61_001155"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP7970282.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IVY61_001156"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IVY61_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP7970283.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IVY61_001157"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IVY61_001157"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP7970284.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IVY61_001158"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IVY61_001158"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP7970285.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IVY61_001159"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IVY61_001159"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP7970286.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IVY61_001160"
     CDS             21905..22483
                     /locus_tag="IVY61_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7970287.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IVY61_001161"
     CDS             22535..23017
                     /locus_tag="IVY61_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP7970288.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IVY61_001162"
     CDS             23134..23508
                     /locus_tag="IVY61_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970289.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DACCXM010000035        23654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCXM010000035 DACCXM010000000
VERSION     DACCXM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.037"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(27..602)
                     /locus_tag="IVY61_001163"
     CDS             complement(27..602)
                     /locus_tag="IVY61_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7970290.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(607..1581)
                     /locus_tag="IVY61_001164"
     CDS             complement(607..1581)
                     /locus_tag="IVY61_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HAP7970291.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1605..1649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1864..2832
                     /locus_tag="IVY61_001165"
     CDS             1864..2832
                     /locus_tag="IVY61_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7970292.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2929..3222
                     /locus_tag="IVY61_001166"
     CDS             2929..3222
                     /locus_tag="IVY61_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7970293.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3340..3576
                     /gene="secG"
                     /locus_tag="IVY61_001167"
     CDS             3340..3576
                     /gene="secG"
                     /locus_tag="IVY61_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP7970294.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3688..4434
                     /locus_tag="IVY61_001168"
     CDS             3688..4434
                     /locus_tag="IVY61_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7970295.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4437..6794
                     /gene="rnr"
                     /locus_tag="IVY61_001169"
     CDS             4437..6794
                     /gene="rnr"
                     /locus_tag="IVY61_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP7970296.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6816..7280
                     /gene="smpB"
                     /locus_tag="IVY61_001170"
     CDS             6816..7280
                     /gene="smpB"
                     /locus_tag="IVY61_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP7970297.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7582..8301
                     /gene="atpB"
                     /locus_tag="IVY61_001171"
     CDS             7582..8301
                     /gene="atpB"
                     /locus_tag="IVY61_001171"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP7970298.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8354..8569
                     /gene="atpE"
                     /locus_tag="IVY61_001172"
     CDS             8354..8569
                     /gene="atpE"
                     /locus_tag="IVY61_001172"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP7970299.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8660..9184
                     /gene="atpF"
                     /locus_tag="IVY61_001173"
     CDS             8660..9184
                     /gene="atpF"
                     /locus_tag="IVY61_001173"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP7970300.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9171..9713
                     /locus_tag="IVY61_001174"
     CDS             9171..9713
                     /locus_tag="IVY61_001174"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP7970301.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9736..11292
                     /locus_tag="IVY61_001175"
     CDS             9736..11292
                     /locus_tag="IVY61_001175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP7970302.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11306..12208
                     /locus_tag="IVY61_001176"
     CDS             11306..12208
                     /locus_tag="IVY61_001176"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP7970303.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12231..13637
                     /gene="atpD"
                     /locus_tag="IVY61_001177"
     CDS             12231..13637
                     /gene="atpD"
                     /locus_tag="IVY61_001177"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP7970304.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13651..14073
                     /locus_tag="IVY61_001178"
     CDS             13651..14073
                     /locus_tag="IVY61_001178"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP7970305.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14542..14772
                     /locus_tag="IVY61_001179"
     CDS             14542..14772
                     /locus_tag="IVY61_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP7970306.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14869..16170
                     /gene="murA"
                     /locus_tag="IVY61_001180"
     CDS             14869..16170
                     /gene="murA"
                     /locus_tag="IVY61_001180"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7970307.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16183..16359
                     /locus_tag="IVY61_001181"
     CDS             16183..16359
                     /locus_tag="IVY61_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7970308.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16494..16790
                     /gene="yidD"
                     /locus_tag="IVY61_001182"
     CDS             16494..16790
                     /gene="yidD"
                     /locus_tag="IVY61_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP7970309.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16854..17321
                     /locus_tag="IVY61_001183"
     CDS             16854..17321
                     /locus_tag="IVY61_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7970310.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17406..18290
                     /locus_tag="IVY61_001184"
     CDS             17406..18290
                     /locus_tag="IVY61_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7970311.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18407..18640
                     /locus_tag="IVY61_001185"
     CDS             18407..18640
                     /locus_tag="IVY61_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP7970312.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18728..18808
                     /locus_tag="IVY61_001186"
     tRNA            18728..18808
                     /locus_tag="IVY61_001186"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18762..18764,aa:Tyr,seq:gta)
     gene            18815..18886
                     /locus_tag="IVY61_001187"
     tRNA            18815..18886
                     /locus_tag="IVY61_001187"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18847..18849,aa:Gln,seq:ttg)
     gene            19402..20109
                     /locus_tag="IVY61_001188"
     CDS             19402..20109
                     /locus_tag="IVY61_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7970313.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20168..20812)
                     /locus_tag="IVY61_001189"
     CDS             complement(20168..20812)
                     /locus_tag="IVY61_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP7970314.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20939..21226
                     /locus_tag="IVY61_001190"
     CDS             20939..21226
                     /locus_tag="IVY61_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970315.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21334..21654
                     /locus_tag="IVY61_001191"
     CDS             21334..21654
                     /locus_tag="IVY61_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7970316.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21972..23048
                     /locus_tag="IVY61_001192"
     CDS             21972..23048
                     /locus_tag="IVY61_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7970317.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23045..23654
                     /locus_tag="IVY61_001193"
                     /pseudo
     CDS             23045..23654
                     /locus_tag="IVY61_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
BASE COUNT         7820 a         4125 c         5092 g         6617 t
ORIGIN      
        1 tattttgcta atccagctct gtttattcag cctatttttc tttttgaata tatcaagcgt
       61 tgtggcaaca tagtgatgac taatgaaaca acgacgatag acaacaaacg atcaaggtag
      121 tctgtacctg cctgtaccaa gatcacactt gttgtctgac ttaaacctaa cccatgtaat
      181 agctgaacga tcatactgga tcctgaggag gtgatcccgc caaataactg aacggtaatg
      241 atcgaagaaa cgatcgttcc aggtaccgac aaaatcaaag cgagtaataa cagtttcaag
      301 ctgtttttat ctttgccgag atatcttggc aaaatcaatc cagcaagcaa gcctgtgaga
      361 atttggattg gcgaataata cagagaaaaa atatctgtag tcactccact taaaataccg
      421 ctggacaatc ctgtcagcat tccaggtaca ggcccaagca atgcaccagc aaaaatcgtt
      481 ccgatagaat cgagatagat tggcaaacga agccataaag caatcgttcc tccaatataa
      541 ttgatcccaa tacacaaagc catcactgta agtacgcgag ttgccatttt gtttttattc
      601 attttgttag cccatcttta acctttccaa atctttcata atcgtttctt cttgtgcatt
      661 taaaagaacg gttaaaaact ttcggaaaaa aagacgagta tctacttttg tcataatttt
      721 actgttagca ggtttctgcc aaaattcata acgatcaacc aatgtttgtc cacgacttat
      781 tccttgagtt tccacagcag tgaagctttt gaaaccttca cagatggcct cctcgataaa
      841 ataagcaaca gctaacggat cattgatgac acatcctaaa attctttctt gtttccagtg
      901 gaagtcaaaa taaaaacgag tgatcgcttt tagatactct ccctcttcag gattcatttg
      961 acagcagtat tcgaggatag tcggtgtcaa gacgatttct cttgtcacat ctaacccaac
     1021 catttctatc gtttgtttga gattctcgaa tacataagaa gctgcttcag gatcacacca
     1081 gtaattgtat tcagcaactg gcgaacagtt accatggctt ttgtaagttc cacccatcga
     1141 gacaaaacgt tccatatgtt tcccaatatt tgggttgact ttcaaagcag aagcgatatt
     1201 ggtcaaagga cccaaagcaa tgactgatgt atttgtcttt tctttgaaat aatcagccaa
     1261 aaaatcaacg gcatggagag gttctgcctg ttttctcaag atcatcggga aatttgtttc
     1321 ccctaaacca tccataccat gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt
     1381 ttccattcct tgataaacag gaatgtctaa acgatcacag cgttccaggc atttcagcac
     1441 attctgcgta cctatatctg cagggacatt cccacaaact acggtaatag caactacttc
     1501 gatatcaggg gatttgacag ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca
     1561 atcaataatt acttttcgca ttatttccaa tctcctttct ttagagatac ttagtttttt
     1621 attctgggaa gtttccgaac cagtccaaga cgagcgtctt gattataatg cacgaaatta
     1681 gtatactggc ttttattgcg aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa
     1741 ctttaagtaa atgatacaat gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat
     1801 cctatggatc ggtacattgg ataatagata gattatagtt tggcagaaag cggcgggtgt
     1861 acaatggtta ttttttattt cattcttatt ctgataatta tagggatcag ttaccatttt
     1921 ctttcttatt tttatgcaaa gaaattagta agagcgggag tacagagagg atatgactgg
     1981 tatgaagaca ctggtcatcg tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa
     2041 cgacaaaaat tagaagaaga gtgtgaaagc ttttgggagc agggaacacc caaaacaatc
     2101 aaaagttatg acgggttgaa actagctggt cagatgttca tccatccaaa taagcagaat
     2161 aaatgggtca tttgcgtaca tgattaccga agcacaggga aaagagacat gtctcatata
     2221 ggtaaaagat atgcagagaa aggattcaac gttcttgtac ctgatttacg tgcgcacgga
     2281 gaaagtgaag gagaaatcat cggaatggga tggctagatc ggctagattt aattgcctgg
     2341 atccaactca tcttagatga acagccgaat gcgtccatta ttttacacgg cggatcaatg
     2401 ggagcttcta cgatcatgat ggcaagtggt gaaaaacttc catcagcggt caaaggattt
     2461 attttagaca gcggatatgt ttctgtttat gcagaattcc gatatatgtt aagtaaaatc
     2521 actgtctttc ctaaaaaaat ggtcatgaga tatgcgaatc attatgccca gaaatacgcc
     2581 ggttactcct taaagcaagc atcagctacg agacagcttg gcagcaatca tctgccttta
     2641 ttagtcatcc atggtgaaag agatcatttt gtccctacag aagcagcgta cacgattcaa
     2701 aatgctacgg caggggataa agcactgctg cttgttccgg aagcagaaca cttggaagca
     2761 tcgatgaaag atccgaaaac ctattggaca gtgatttttt catttattga acaaagaata
     2821 acgatgaatt aattttctct tcttttttgt aagtaattcg aggataaata attgtttata
     2881 gatcaggaga ctgctaaaat aaggtaggaa taaaaaaata tcagttattt gaggagtgcg
     2941 gcatatggaa atcaaagaag aaaaaatcgt cttgctttat tcaatgatga acaacaagag
     3001 atcggagaaa tgacatggtc agatgcgggc ccagacatca tgatcgttga ccatacattt
     3061 gtagatccag catatcgtgg tcaaaagtta gctgaaaagc tagtgtatac tggagtagaa
     3121 ttagcaagac gagacgggaa aaagatcatt cctttatgcc cttatgcaaa aaaagagttt
     3181 gaaaagaaac cagaatatca agacgtctta cgtcagtcat aagaataaca gctgaaagtt
     3241 ttgaactttc agctgttatt ccatttatat agagttgtca cggactttta tctatgataa
     3301 agttaaagag acgatatctt ttgggaagga ggctgcgata tgtataattt aattttggga
     3361 atcgtgatcg ttttatcgat tgttatggtc attgcgatta tgatgcagcc aagcaagcaa
     3421 aatagtgcag cgagtgcatt taccggcggt gcggatcaac tattcggcaa acaaaaagct
     3481 cgcggctttg aagctgtcat gcagcgatca actgcagtta tgggagctat ctggatgatc
     3541 ttattatttg tcctagcata tttatcttcg aagtagttga ataagcctct cgcaaaacgg
     3601 cgggcggctt tttttattgc aaagagaacg cttaagaaga agttatgaaa gaaactatgg
     3661 taaaatgagg cgaaagaggt gagacgagtg agacaattac caaaacccct ttatgcaaaa
     3721 catggaaagc gagcggtttt gctgcttcac gcttactcag gaagtccaaa cgatgttcga
     3781 atgctcgcac gatttttgga aaaagcagat tatacagttt atgcgccgct ttttaaagga
     3841 cacggaacaa tggagccata tgatatcttg caagaaaaag cagaaagctg gtgggcagac
     3901 actaaaaaag ccatccattt tttacagtct gagcagtttt ctgatattgc cgtattagga
     3961 ttatcaatgg gcggcatctt tgctgtacga gcactagaag aagaatcagt gattggcggc
     4021 ggttttttct gttcgccttt gtctcctgta aaaacaaacg tgcctgaaaa ctttgaaaaa
     4081 tacgtacgtc aagtgctgaa gacagcaggt aaatcagaga aagaaatcaa tgaaaaagca
     4141 gttgcttacc gctcgttagc tgaacaacaa ttaatggata tacaagatca ggcagcaatc
     4201 gtagaaagta ggttgtcaga tatccaacag ccgatatttt tagctcaagc tgggaaagat
     4261 gaaatgatcg accctaatgg cgtatttgaa actgcacgca agttgagccg tcagcgagtg
     4321 acacttcaat ggtatcctga aagtggtcat gtgattacag ttggtacagc aagacgcgaa
     4381 ttagaaaaag acgtcttgga atttttagaa aaattacctt ggaatgagga ataaatatga
     4441 caaaagaaac gataaaagag aaaattctct ttttcatgga aagcagtcgc aaacgcagtt
     4501 tttctatgga agaaatcgct gaaggtttag ggttccaaaa aagcgatgat tttaaattac
     4561 tagtacaaac gattgctcaa atggaaagag aacagagtat agttttcaat aaaaaaggaa
     4621 aagtgaaact tcctttaaaa ccaatcctga tcgaaggaac atttcgagca aatgaacgag
     4681 gatttggatt tgtaacgatt gatcccgaag aagatgacat ctatatccca aaagaagcga
     4741 ctggttttgc aatggatgga gacaccgtag caatcgatat catgaagcga gcaaatacag
     4801 atatggatcg cggagcagag gggaaagtag tcgagattcg agaccgggcg actactcagt
     4861 tagcagggga attcgttgct tattcagaag aagaaataca agaaacagat ctttacggcg
     4921 ttgttatccc gaaagataga aagctcaacc aatttaaagt atatgcagca gcagagggag
     4981 ttcgtccagt tgacggaagt attgtgctga tcgaactgac ccattaccct gaaaaagggt
     5041 atgcgactag tttagaaggg ataatcaagc aagtgattgg tcataagaat gaccctggca
     5101 tggatatcct ctcaattgtc gtggctaatg gtatccctac gaaatttcct gatgatgtat
     5161 tgtctgaagc agatgaagtt ccagatcaaa tcagcgaaaa ggatataact ggacgtagag
     5221 atttaagaaa tcagctcatt gttacgattg atggagaaga tgcaaaagat ctagatgatg
     5281 cagttacggt taaaaaatta gataacggta attattttct tggagtacac attgcagatg
     5341 tttcttacta tgtgacacaa ggcagtcaat tggacagaga agcatatgaa agagggacaa
     5401 gtgtatattt gactgatcga gtaattccga tgatcccgca acgtttatcg aatggtatct
     5461 gttcactgaa tccccatgtt ccaaggctga ccatgagttg cgaaatggag attgatccaa
     5521 atggacaagt agtctctcat gatattttcc caagtgtgat ccaaacaaca gaacggatga
     5581 cctacacagc tgtcaatcaa atcttagaag atcaagacga agaaacgatg caacgctatg
     5641 ctcagctcgt acctatgttc caagaaatgg gtgaattgca ccgaatcctt gaaacgatgc
     5701 gaatcaaacg tggcgcgatt tcttttgagg atcgcgaagc gaaaatcctt gttgatgctg
     5761 aaggacaccc aaatgacatc cagctaagaa gcagaggaat tggtgaacgc ttgatcgaat
     5821 ctttcatgct tgcggccaat gaaacggtag ctgaacatta taataaatta aatctgccgt
     5881 ttatttaccg aatccatgaa cagccaaaag aagaaaaaat gcagcgcttc tttgattttg
     5941 cttcagcttt gggcattctg gtccgaggaa cgaaaggaac gatcacacca aaagatttac
     6001 agcatgtgat cgaaggagta gaagacaaac cagaagctgc agttatcaat actatgttgc
     6061 taagaagcat gcaacaagca cgctattcag aagacaattt tgggcattat ggcttagcgg
     6121 cagattacta cacacacttt acatctccta ttcgacgcta tcccgatcta atcgtccatc
     6181 gtctgatccg ttcttacagt gaagataaaa gtgaaagcaa tcaaagttcg tgggctgcgg
     6241 aacttcctga aattgcagat catagttcta agatggaacg tcgtgcagta gaaacagaac
     6301 gtgaagtaga ttccatgaag aaagcagaat tcatgatgga caaagtggga gaagaattcg
     6361 atgggatcat cagctctgtt gtgaaattcg gcctgtttat cgaactgccg aatacagtag
     6421 agggattgat ccatatcaat gaattgaaac aggactactt ccattttgtt gaaaatcatt
     6481 tagctcttgt aggtgaaaga actggtctga cttttaaaat cggacagaaa gtgcgagtaa
     6541 aagtagtcaa agctgatcca gatgaacgag cagtagattt tgaacttgtt tccgcagaag
     6601 aggttacagc gttggaacgg ccaaaaacac gaaatggaaa atcaggaaaa cgcgaaggaa
     6661 aacaagcagg aaaacgccca gaaagaaatg gcaagaagaa caaacgatat agcgatagta
     6721 agcaaacgtt cggtaagaaa aaagggaaga agccatttta caaaggtgtt aaaaagaaga
     6781 aaaagaaaaa ataaaatgag ttggaggaaa cagctatgcc aaaaggcgag ggaaaattaa
     6841 ttgcacaaaa caagaaagct cgccatgatt attcgatcat cgacacgatg gaagcaggga
     6901 tggtcttgca aggaaccgag atcaagtcga tacgaaacag ccggatcaat ctaaaagatg
     6961 gatttattcg cgtccgcaac ggagaagctt tcttgcataa tgttcatatc agtccttatg
     7021 aacaaggaaa tatttttaat catgatccgt tgcgcacgag aaagttatta ttacacaaaa
     7081 aacaaatcat ccggcttgaa aatgaattga aaaatactgg aatcactgtt gttcctttaa
     7141 aagtctatat tcgtaacggc tatgccaagg tattgattgg tctggcgaaa gggaaaaaat
     7201 cttatgataa acgggaagat ttgaaacgaa aagatatcga tcgacaaatt gatcgaacat
     7261 taaaaaattt ctctagataa tttcattatc ctttttcgca tattcggtgc ttttgctttt
     7321 acttaatgat aaaatttgtt agcatttaat ctgataaaac atgattatca taggtttttt
     7381 cttagatcag gagcaccgtg tataaagaaa aattcgcttc gtttttgact gaaataaaaa
     7441 agtgtctaat tttcatgaaa atgttttgaa ttttctgttt catggtggta tgataccgtg
     7501 tggttagaaa ccgattggaa aaaaccagat acgcacatga attgatttta tgtgaatttc
     7561 ggggaaaaga ggtgaaatgg attggatgaa cgctcgctaa cgttccacat tggaccaatt
     7621 tggtttgatg gtacagtttg tataatggtg ttactgacct gtttgattgt tttcttctta
     7681 gcttactttt ttacaagaaa tcttaagttg aaaccaacag gcaaacaaaa tgcgatagaa
     7741 tgggtcattg attttagtcg aggaatcgta acagataatc tgcctagaaa agaattgagc
     7801 aatttccatt tgttggcatt tacattgttt ttatttgttt ttgttgcaaa caacatcggt
     7861 ttagtaacaa aaatcgtttt acctaacgaa acaacacttt ggaaaagccc gacagcagac
     7921 ccgtttgtta ctttaacttt ggcatttata atgatcttac ttactcactt attcggggtg
     7981 aaaaagttag gcttcaaagg atatttcgta aattcgttct tgaaacccta tagttttatg
     8041 tttccgatga aactgattga agaatttaca aacttattaa cacttgcttt acgtctttat
     8101 gggaatattt atgcggggga agttttgcta accttgattg caaaaatgat gacaagctta
     8161 ggctggttct cattaccatt agcaattcca ttagagatgg tctggatcgc gttctccttg
     8221 tttattggga gcatccaagc atttgtcttt gtcactttgt caatggttta tatgagccat
     8281 aagattgaag tagaagaata atactcggtt aaaaaacaaa ctactattta tctattttag
     8341 gaggaaaaca attatgaatt atatcgcagc agcaatcgca atcatgggag cagcaatcgg
     8401 agccggttat ggtaacggtc aagttatctc taaaacaatc gaatcaatgg ctcgccaacc
     8461 tgaaatgtct ggtcaattcc gtacaacaat gtttatcggg gtagctctag ttgaagctgt
     8521 tcctatctta ggggtagtta tcgccttgat tctagttttt gccgtttaat tgaatcagta
     8581 ttaaggcaga aacaacatcg tttgttactg cttttttaca tggaaactag aaatactcca
     8641 aagaaaggaa ggctcgccta tgctgaatca attggcaatt gcagaagttg gcaatgcgat
     8701 gttaggtaat atcatcgttg tcagcggctc atttttgata ctactggctc tcttgaagca
     8761 ctttgcttgg ggaccaatca gcgatatttt gaaaaaacgt gaagacaaga tcgccaatga
     8821 tttagattct gcagaacaat ctcgcatcaa ctcagcgaaa atggaacaag aacgcgaaca
     8881 acaattgtta gcctctcgtt ctgatgcagc tgatatcatc aaaaatgcga aagaaagtgg
     8941 agaattaagc cgccaaaata ttttgaagga tgctcaagaa gaagcagctc gtctaaaaag
     9001 caaagcccaa gctgatatca ctgtagaacg tgattcagcg ctgaactctg taaaagacga
     9061 cgttgcagaa ctctctcttc aaatcgcgga aaaaatcttg aacaaagaat tatctccaga
     9121 aatgcatgaa tcattaatca atcaatacat tgaaggtcta ggttcttcaa atgaaactag
     9181 ataaatatac agtaggaaga cgttacggga aagctttatt tgaactggcg atcgattcaa
     9241 atcaagcaga agaaatctat caagatctcg taagtttacg tcaaatctat gaacaagtac
     9301 ctggattagg gaatatgtta agtgatgttc gtctagaacc tcatgaaaaa cgagaaatca
     9361 tggacaagct tgtcagcggt tatgatggga tcgttaaaaa ctttttagaa gttgtttatg
     9421 agtataaccg aatggatgac cttttgttca tgatcgaaga atatgaacgt cgatatgatg
     9481 aacatcaagg gttactattg ggaagtgtca caacagcagt cccactttca gacgaacaac
     9541 gaatacaatt agaaaagaat gttgcaaaaa caatggacta tcaatcagtt gaattaaaac
     9601 aaatcgttga ttcttctatt attggtggcg cgattgttga agccaaccat cgagtaatcg
     9661 acggaagcat tcgcacgcaa ttagaaaaaa tgcgtaatca attaaacaga tagtacaaag
     9721 aggtaggtga atcgaatggc catcaaagca gaagaaatta gtgccttgat taaagaacaa
     9781 attgaaaatt atcaaaacgt gctttcagtc gaagaaatcg gtaccgtaac ttatgtaggt
     9841 gacgggatcg ctcgtgcaca tggtttagaa aacgcgatga gtggtgagtt gcttgaattt
     9901 tcaaacggct catacggaat ggcgcaaaac ttagagtcaa atgatgtagg tatcattatc
     9961 cttggcgatt tcgagtccat tcgtgaagga gataaagtaa aacgaacagg taagatcatg
    10021 gaagttccag ttggagaggc cttgatcggt cgggtagtca atccgctagg tcaaccaatc
    10081 gacggactag gtgaaatcgt tactgacaaa gctcgtccag tagaagcgat ggcaccaggt
    10141 gttatgcaac gtaaatctgt taacgaacca atgcaaacag ggctaaaagc gatcgacgcc
    10201 cttgtgccaa tcggacgcgg acaacgtgaa ttagtcatcg gtgaccgtaa aacagggaaa
    10261 acttctattg cgatcgatac gatcatcaac caaaaaggtc aagatatgat ctgtatctat
    10321 gtagcaatcg gacaaaaaga ttctacagtt cgtacacaag ttgaaacatt gaaaaaatat
    10381 ggcgcaatgg attacacaat cgttgtgaat gccggtgcgt ctcaaccagc accattgctt
    10441 tatatcgcac catatgctgg tactgcaatg ggtgaagaat tcatgtacaa cggtaaacat
    10501 gtattgatca tttttgatga tctttcaaaa caagctgttg cttatcgtga actttccttg
    10561 ctacttcgcc gtccaccagg tcgtgaagct tatcctgggg atgttttcta cttgcactca
    10621 cgtttattag aacgtgcagc aaaattaagt gatgatttag gtggcggttc aatgactgcc
    10681 ttaccatttg ttgaaacaca agctggagat atctctgctt atatcccaac aaacgttatt
    10741 tcgatcactg atggacaaat cttcttagaa agcgacttgt tctacgcagg tactcgtcca
    10801 gcggttgatg ccggactatc tgtatctcgt gtaggaggtt ctgcgcaaat caaagcaatg
    10861 aaaaaagttg ctgggacttt acgcctagac ttagcaagtt accgtgaatt agaagcattt
    10921 acacaattcg gttctgactt ggatgcagca acacaagcaa aactaaatcg tggacgccgt
    10981 acagtagaaa tcttgaaaca aaaattgcat gcaccgctag ctgttgaaaa acaagtagtg
    11041 attttgtatg ctttgacaca tggattctta gatagtatcc ctgtagacag tattttagac
    11101 ttcgaacatg aattattcga atatctggat accaatcatt cagatatctt tgaaacaatc
    11161 cgtacaacaa aagatcttcc agaagaagaa gcgttgaaca gtgcgattca ggaatataaa
    11221 gacatgttct tagctacaaa aggaagtaat tcctctacag aagataaatt gaaatccatt
    11281 caaaatgcat agtgaggtga gatgaatggg tgcttcatta aatgaaatca agacacgtat
    11341 cgcttcaacg aaaaaaacga gccaaatcac gcgggcaatg cagatggtgt ctgcttctaa
    11401 gttgacaaaa tctgaagctt cttcacagaa gtttcaggtt tatgcaacaa aagtaagaga
    11461 aatcgttacg catttgactg ctactcagtt aaatgatatt gcctcagaaa atcctagagg
    11521 ggatatcaat tacaacagca tgctgctcag ccgtcctgtg aagaaaactg gttatatcgt
    11581 catcactgca gatggcggtt tagtcggtgg atataacagc tcgatattga aacaaacgat
    11641 gtctatttta gaagaagacc accaatcacc cgatgattac gtgatgatcg caattggcgg
    11701 aactggtgca gacttcttta aagcacgcgg tatcaatcta gcatatgagc tgcgcaatct
    11761 atctgatcag ccaagctttg atgaagtccg caaaattgta aatatggcaa caactatgtt
    11821 ccaaaacgaa gtattcgatg aattatacgt atgttataac catcatatca attcgctaac
    11881 tagtcagttt cgtgtagaaa aaatgctgcc aatctcagac ttagatccag aagaagcaac
    11941 gactttcgaa caagaatata tttttgaacc ttcaaaagaa gaaatcttgt ctcagttatt
    12001 gcctcagtat gctgaaagtt tgatctacgg tgcgatcgtt gacgccaaaa cagcagaaca
    12061 tgctgctggt atgacagcaa tgaaaacagc aactgataat gctgctaata tcattgatga
    12121 gctgacggtt tcttataacc gtgcaagaca aggggctatt acccaagaaa ttaccgaaat
    12181 tgttgccgga gcttcggcac tagaatagta ttgagattgg aggaaaaaag atgagttcag
    12241 gcaagattgt tcaagtaatc ggtcccgttg tcgacgtgga attttcttta gaccaatcct
    12301 taccagatat caacaacgca ttagttgttt ataaaaatga cgaaaataaa tcaaaagttg
    12361 ttcttgaagc agcgttagaa ttaggggacg gagtgatccg gaccattgcg atggaatcaa
    12421 cagatggttt acaaagagga atggaagtca ttgatacagg caaagcaatc tctgttcctg
    12481 taggtaaaga aacattaggt cgcgtattca acgtactagg agatacgatc gatttagaaa
    12541 cacctttccc agaagatgcg gaaagaagcg aaattcataa aaaagcacca gcctttgacg
    12601 aattaagtac aagtacagaa attttggaaa cagggatcaa agttatcgat ttgcttgccc
    12661 catatttaaa aggtgggaaa gtcggactat tcggtggtgc cggtgttggt aaaaccgtac
    12721 tgatccaaga actgatccat aatatcgccc aagaacatgg tggtatttct gtatttaccg
    12781 gtgtaggtga acgtactcgt gaaggtaatg acttgtatta tgaaatgaaa gattcaggag
    12841 ttatcgaaaa aacagccatg gtgttcggac aaatgaacga accaccaggt gcacgtatgc
    12901 gtgttgcttt gactggtttg acgattgcgg aatatttccg tgatgtagaa ggtcaagatg
    12961 tactgttgtt tatcgacaac attttccgtt tcactcaagc tggatctgaa gtatcagcct
    13021 tgttaggacg gatgccttct gcggttggtt atcaaccaac attggcaaca gaaatgggtc
    13081 aattgcaaga acgtatcaca tctacgaaaa aaggttcgat cacatcgatc caagcaatct
    13141 atgtgccagc cgatgactac actgacccgg cgccggcaac agcattcgcc catttggatg
    13201 cgacaaccaa cttggaacgt aaattaacag aacaagggat ttatccagcg gtagatccat
    13261 tggcttcttc ttcaagtgct ttagcacctg aaatcgtggg agaagaacat tataaagtag
    13321 caacagaagt ccaacacgta ttgcaacgat atcgtgaatt acaagatatc attgctatct
    13381 taggtatgga tgaattatcc gatcaagaaa aagtgttagt ttctcgtgcc cgcagagtcc
    13441 aattcttctt gtcacaaaac tttaacgtgg ctgaacaatt tactggtctt ccaggttcat
    13501 atgtccctgt agaagaaaca gtgaaaggat tccgcgaaat cttggaaggc aaatacgatg
    13561 atcttcctga agaagctttc cgaagtgttg gccgaatcga agacgtcgta gaaaaagcga
    13621 aaacactagg ctactagaaa ggggtgcccc atggatcaat atttaacagt aaatgtggta
    13681 actcctgccg gtttggtcta tgatcaccat gcagctatcg tcgttgcacg tacgacagct
    13741 ggtgagcttg ggattttgcc taaacatgca ccaatcatcg ttccattgac gattgatgaa
    13801 gttcgtgtaa aacgaacaga ttcagatacc cacgtcgatt ggatcgctgt taatggcgga
    13861 atcatggaag ttcgtgataa tgttgtttca atcgttgctg atagtgcgga acgtgaacgt
    13921 gatatcgatg tatctcgtgc ggaacgtgca aaacaacgcg cagaacgcca aatcgaagaa
    13981 gcaaaagcaa aacaagatac gaacgaattg aaacgggcaa ctgttgcgtt gcaccgagca
    14041 atcaatcgta tcaaagtatc taagcactca taaaatgaga tgcggatgtt tatccgtgtt
    14101 taagaacagt aaaaagaggt tatgacagaa gtggtcagct tcaagacata agaaggaatt
    14161 tacgaaaatt gcttctcaaa tttttgtgaa atctcagctt atttttcgaa gaagctactt
    14221 ctggaacacc gtatatcaga ggttgtgaaa tcagcgtttt gtcctgagta ttagagaaac
    14281 aaatggaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagatga
    14341 ttgaacaccg tttatttgga ataagggact gcgacaagac ttttgtctca gtcccttttt
    14401 ttgcatattt ttagcattta tttcctaaaa aatgtaaggg gattcctata tttttaagta
    14461 tacttgtttt tccttttata cattttatgg tattatcatt ttattagtta ttataaaaca
    14521 atcgttagaa aggacgtcat tatgcaagta tacggaattg atgcggtgct tcgaatcgtg
    14581 agtcatttgg cgtttatcta cttagccttc tggtctttac gttcgttacg cattgaaact
    14641 tttttcagac caatgcataa tacgcaaatt agaatggcaa tcgtactttt ttcaattttt
    14701 ttagggtaca ctgcaagtaa ttttttctta gaggttatcg ctttatgccg aaatcttttc
    14761 gttagcctgt aaattttaaa aaaaggtact attttaaaag caaggatgtt ataatagaaa
    14821 agattttgag taagtgtatg ctcattggaa ttttttggag ggaacaacat ggaagagatc
    14881 atcgtaagag gtggcaatca attaaatgga acagtaagaa ttgaaggagc taaaaatgct
    14941 gttttgccta ttttagctgc tagtttgtta gcagaagaag gtattacgac attagacaac
    15001 gttccgattc tttctgatgt cttcactatg aatcaagtga ttcgtcactt aaacgtagat
    15061 gtagattttg atgaacaaaa gaatcaagta acaatcgacg cttcaagaca attagaaatc
    15121 gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta
    15181 cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat tggtaaacga
    15241 ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat tcaaaaaaac
    15301 ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt agatttccct
    15361 agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc
    15421 attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt gaataaaatg
    15481 ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt ggatcattta
    15541 catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt tatggttgct
    15601 gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca taaccgccca
    15661 ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg cggagtccgt
    15721 gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca tcctggtttc
    15781 cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg tacaagtgtg
    15841 gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg ccgtatgaac
    15901 gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga attgcaaggt
    15961 gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc tggcttgaaa
    16021 gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata ttataatttc
    16081 catatcaaac tacagcaatt aggtgcagac gtcgaacgtg tagacatgga tcaaacttct
    16141 gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa ccaacgatat
    16201 atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct tctttttgtg
    16261 gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa agtattccag
    16321 ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat cacagtggtt
    16381 ctagtcgata tgaagtgagc catgaagcag aacatgataa aagaaaacag aagggaacgt
    16441 tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa ttgatgaaga
    16501 agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca tttccaccta
    16561 gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa acccatggtg
    16621 cgctaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct tttgtcaaag
    16681 gcggtatcga ttatgtacca ttgacattcc gattaacgcg aaataagaaa gataaaaaag
    16741 aaccagatta taaaaaggtt gtgaaaaata cgggtagcac tgagcgataa agaaaaagag
    16801 gaaatttatt gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg
    16861 aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag
    16921 aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa
    16981 caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag
    17041 taaccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga
    17101 agctttcaca gctagcttca aaccaaactc ggatcattat acgtactggc actactagta
    17161 gcgctgcgtt acacctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact
    17221 attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag
    17281 tgattttagc aattcactta cctaaaaata gctttaacta ataaactttc aattcgagaa
    17341 aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata
    17401 tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg
    17461 atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga
    17521 aaacgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg
    17581 gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga
    17641 ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt caactcccag
    17701 taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc
    17761 gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca
    17821 cgaaaggaca aataaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct
    17881 tagatggcga taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt
    17941 gtgaacgcaa aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag
    18001 aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg
    18061 gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt
    18121 ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat
    18181 tcgatctgca tgatttcgaa ccgcagattt tattatgtga gtacagaaac gatgctaatc
    18241 ttataggtgc agcagcaaac tatctcgctg tacgtcaagg agagaattaa gagctttaga
    18301 gaaggtgcag cttgcataaa agaagagaat gaaatgaata ggagattttt acctaaggaa
    18361 tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa
    18421 taaagatatt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga
    18481 agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct atgggccaaa
    18541 agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc
    18601 gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt
    18661 caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa
    18721 acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg
    18781 ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa
    18841 gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt
    18901 aagctctcgt tagttcgaga gctctttttt tttgctttgc taccttgttc cttgaagatt
    18961 ctgctgaatt aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg
    19021 aaagaaaaag tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac
    19081 aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc
    19141 ccaacgaatc atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat
    19201 aagtcggcat tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa
    19261 aaaattgttt ctgacaatag gagtagggag aacgatgtgt gtctaaatcc tctgttgatg
    19321 gatagaaacc tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa
    19381 acgaaaggac gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac
    19441 caattaaaaa atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa
    19501 agagaattag cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac
    19561 ttggcagatg aaggaatctt agaaagaaag atcggttcgg gtacgtatgt tgcgcgagaa
    19621 aaagtacaag aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcacaaaat
    19681 cgagtaccgt ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg
    19741 gaaaaactaa acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca
    19801 gatgatgtcc ctatttgttt tgaagttgcc agtgttccac aaaagctgat cgatggctac
    19861 agcaaagcag agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag
    19921 atcggtccag cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat
    19981 ttagaggtaa aacgaagaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac
    20041 ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg
    20101 gaaaaataaa aaacaggtga tccctgattc cttattagaa ataaaagaga atcagaatca
    20161 cctgttttta tatatgatca gttatcagtt gctcgtgata agaaacagaa ccctcttcaa
    20221 attggacttg tccgttcagc agctcaatga tggattcttt aaagtggtta gcactttttt
    20281 catctacagc acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat
    20341 gttggagttc aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga
    20401 tctcctgttg caaggtacct tctacgattc caatgtcaga caatgcttga gagaccgcat
    20461 gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga
    20521 ctactgcagc tacattgatc agttgatttt ttttgaggac ttccaacatc ggtacgcccg
    20581 ccgttccact tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga
    20641 tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaat
    20701 tttttgcttg gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga
    20761 tctcaatctc actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac
    20821 ctcttttttt cattttcttt attcagtata aataaagttc taggttgttt caacactgta
    20881 acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg atctggggat
    20941 gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc
    21001 tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc
    21061 caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg
    21121 tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata
    21181 tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta
    21241 gaaaacgttg acaaatttct ttctttttat ataataaagt aacagacgcg aagaaaacgt
    21301 ttgcgtagtt tgttttaaaa aggagagaaa aatatggcta aaaaaacaat catgcttgta
    21361 tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca
    21421 aaaggaatcg actctgatat tttcgctgtg tccgcttctg acgcagataa caatcttgca
    21481 aataaagacg tggatgtgtt gttattaggc ccacaagttc gctttatgaa agctgatttt
    21541 gagaaacgtc tggaaccaaa aggcattcct ttagatgtca tcaatatggc tgattatggt
    21601 atgatgaatg gcgaaaaagt gttagatcaa gcaatcgcgc tcataggtga ataaatagaa
    21661 cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc gtatattttt
    21721 ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga gatattaaag
    21781 caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca gtggaacgtt
    21841 tgggcaactg gattctttgt catcgctgtg gaatgaggac gtccgtcatt gttggacagc
    21901 ttgcagatta cggttatttt tgtacacatt gtctatattt agggtgatgc gacactcaac
    21961 aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc tttttttgga
    22021 caggacagct tactgaaaaa caaacggaaa tcgcaaagag aatactttgc cattcagaaa
    22081 aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg ctttatccta
    22141 ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg
    22201 tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc
    22261 tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt acgattcatc
    22321 agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc gatgcgtttc
    22381 cttataacgg ggatgctggg ctagctcacg ctgtgcaaac agcaatcaga tcaaacggcc
    22441 gattcatcta cttaagtgcc acgcctgatg agaagttact aaaggaaatc aagcagacat
    22501 ttgagctcca caaacttgcc ttgcgctttc atcggcgctt gcttcctgaa cccgtgcttg
    22561 ttttcatgaa taattggcga caaaaatgtg gaaacaaaaa gaaaatcaac tctttggtgc
    22621 ggatcattca gcaactaatc aaagaaaacc gtatcctttt attttgtcca agtattacga
    22681 tgatgcatcg tttaaaaatc acactgcaag aagcttttcc agaatatgag ataacagatg
    22741 tttcttcaca agacgatcaa cgggcaaaga aagttcagaa aatgcgagaa gcaatatacg
    22801 agatactgtt aaccacaatg atcctagaaa gaggagtcac atttgaaaga atctctgtca
    22861 tcgttttagg agcagatcat ccagttttta ctaaatcctc tttggtgcaa atcgcaggaa
    22921 gagctgatcg taaaggtcct tatacgaaca gtcgagtcta ttttttctat gaagaaaaaa
    22981 cttcagctat tcgaaaagcc tgtcaggaaa tcaaagaaat gaacgaagaa gggaagcaga
    23041 tgctgtgaga tgtagttgct gcggtaaaga catcattcaa aatttaacat tcagcgagat
    23101 catcggagtg tcgaaatcta gacttcctca gacatgttca gtttgccgcg cacaattcca
    23161 tttattagaa gcaaaaggtt gtgcaggttg tcaaaaacca atccatttga aatacgagga
    23221 gacgaaaaag aaagcaaata ctagtttcgt ttactgcaac gactgtcaag aatggaagaa
    23281 gaaatatcct gcatatgatt ttcaacaccg cgcctttttt acgtacgatg aaaagatgca
    23341 ggcttggatc aatcggtaca agtttatggg agatatccga ttagcaggga cttttgcata
    23401 taaatggact cagataaaaa aaagattacc cggcatatat ttattgtcca atacctttat
    23461 ctgaaaaaag gtggctagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa
    23521 aaattccttt tcagatgctg ctcaaaagag agcaacatct gcttgctcaa gcaaaaaaca
    23581 cgcgaaagga aagattggag atgcctcagc cttttgagct tgccgtacct ctttctaaga
    23641 tacaacataa aaaa
//
LOCUS       DACCXM010000036        23613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCXM010000036 DACCXM010000000
VERSION     DACCXM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.038"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            57..1397
                     /locus_tag="IVY61_001194"
     CDS             57..1397
                     /locus_tag="IVY61_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7970318.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1615..2058
                     /locus_tag="IVY61_001195"
     CDS             1615..2058
                     /locus_tag="IVY61_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP7970319.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2562..3980)
                     /gene="nhaC"
                     /locus_tag="IVY61_001196"
     CDS             complement(2562..3980)
                     /gene="nhaC"
                     /locus_tag="IVY61_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7970320.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4383..5606)
                     /locus_tag="IVY61_001197"
     CDS             complement(4383..5606)
                     /locus_tag="IVY61_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP7970321.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5637..6476)
                     /locus_tag="IVY61_001198"
     CDS             complement(5637..6476)
                     /locus_tag="IVY61_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7970322.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6493..7173)
                     /locus_tag="IVY61_001199"
     CDS             complement(6493..7173)
                     /locus_tag="IVY61_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7970323.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7175..8248)
                     /locus_tag="IVY61_001200"
     CDS             complement(7175..8248)
                     /locus_tag="IVY61_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7970324.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(8895..9566)
                     /locus_tag="IVY61_001201"
     CDS             complement(8895..9566)
                     /locus_tag="IVY61_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7970325.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9653..12331)
                     /gene="mgtA"
                     /locus_tag="IVY61_001202"
     CDS             complement(9653..12331)
                     /gene="mgtA"
                     /locus_tag="IVY61_001202"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP7970326.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12990..13427
                     /gene="ndk"
                     /locus_tag="IVY61_001203"
     CDS             12990..13427
                     /gene="ndk"
                     /locus_tag="IVY61_001203"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP7970327.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13449..14315)
                     /locus_tag="IVY61_001204"
     CDS             complement(13449..14315)
                     /locus_tag="IVY61_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7970328.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14475..16568)
                     /locus_tag="IVY61_001205"
     CDS             complement(14475..16568)
                     /locus_tag="IVY61_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7970329.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17317..17940)
                     /locus_tag="IVY61_001206"
     CDS             complement(17317..17940)
                     /locus_tag="IVY61_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970330.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18099..19496)
                     /locus_tag="IVY61_001207"
     CDS             complement(18099..19496)
                     /locus_tag="IVY61_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7970331.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19528..20499)
                     /locus_tag="IVY61_001208"
     CDS             complement(19528..20499)
                     /locus_tag="IVY61_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7970332.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20873..21298
                     /locus_tag="IVY61_001209"
     CDS             20873..21298
                     /locus_tag="IVY61_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970333.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21786..22487)
                     /locus_tag="IVY61_001210"
     CDS             complement(21786..22487)
                     /locus_tag="IVY61_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7970334.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22503..22943)
                     /locus_tag="IVY61_001211"
     CDS             complement(22503..22943)
                     /locus_tag="IVY61_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7970335.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22943..23518)
                     /locus_tag="IVY61_001212"
     CDS             complement(22943..23518)
                     /locus_tag="IVY61_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP7970336.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         6791 a         4896 c         4063 g         7863 t
ORIGIN      
        1 tgctatttat tcttaatata aattttgatt gtgaatttaa aagaaagagg tcatttatgg
       61 aagataaaaa attgaaaaaa caacttagct ccagacatat caccatgtta gctttaggtg
      121 gagcaatcgg tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc gccggccctt
      181 ctgtattgat tgcttttttg attggtgggc tgattttata tatcgtgatg aaaggtctgg
      241 gaaaaatcgt tttaagcggt ggcgacacgc atcacggatt atctggtttg atacgccctt
      301 atctaggtgc tcattctgct gattttactg attgggtcta ctggtctatg tggattatca
      361 atatcatcgc tgaagctgtt gcagctgctt cctttttgca gttgtggttc ccgcacattc
      421 cagcctgggt atttgttttt atgttggctg ttttaactac gatcatcaat ctttattcgg
      481 tacggttatt cgcagaaaca gaatattggc tggcttttgc taaaatcagt gtcattatct
      541 tactgatcat ttttgctact tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc
      601 ctacccttca gcaattgact gaccatggtg ggtttactcc tcatggtatg aagggaatca
      661 tcagttcttt gcttgtagtt atctattctt acggcggttc tgaactgatt gctatcacgg
      721 taagcgaagc agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa
      781 gaatcatttc cttttacatc attcctttgt ttttgctttt gattcttttt ccttggaata
      841 ctttagcagg cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg aatatcccat
      901 ttgcagcgga tatcgtcaat tttgtgattg tccttgcttt gttctcatct atcaattcgg
      961 gtgtgtatgc atcttcaaga attttgttct tccgtttgaa ggatcgaaaa ggttcctcta
     1021 gaaaactagc tgttttaaac aagcatcaag taccgcaacg tgctgtttta ttttgtacca
     1081 gtgttcttta tcttggtgta gctctttctt attttgtcgg tgacaaatta tttggctatc
     1141 tggcaggttc cctttcgtat acagtattat tgatctggat cattatcagt gcggcatcct
     1201 ttgtactggc actaagaaaa ggaacattat gggataaagg aatcagcttt tttgcattag
     1261 ctgtcttagg attgattttc cttggtatcc tgttcacgaa ctccattggg gtaactgtat
     1321 tgactggcct gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgcgttcg
     1381 tattgacgaa tgaatgaaat cgatagaaaa ttttatttta tttatgtgga atagatggag
     1441 ctattgaaga aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc gaataaacag
     1501 tctcatgacc tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc
     1561 tccatgctca tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa
     1621 aagtattgga agtttttgtt gatgatcggt gtttcaacgg tagttatgtt tggtatgatg
     1681 tacttgaatg tctatgcgct cgatcatatc ttcttcagtc gtacacgagt attcatggcg
     1741 ttgatgatgg gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa
     1801 aacaaaaaga taaatgttat gatcttagcc gctagtcttc ttgtttttgc cggttccttg
     1861 tttatggtac gaagccaaac tactgtcaac gacacagctt ggatgaaggc catgattcct
     1921 catcattcta tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa
     1981 aaattagcgg aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg
     2041 atcaaagaaa tggaatgacc attatccgtc caaataaaaa aagaggatgt tccagaagtt
     2101 atcagcttca agaaataaga agaagctact tctggaacgc cgtgaataag gggttgtgaa
     2161 aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagcttct cctatttttc
     2221 gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt
     2281 aaaatcagcg ttctgacctg agtattagag agacaaatgg aaaaatagtt cctcatattt
     2341 tgccattttt tgagctaata ccgagggtca gctgattgaa caccgtttat cgagaggttg
     2401 tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct ttccacattt
     2461 ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta ttcgtaaaag
     2521 ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc taattgcgct
     2581 gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca acctgtgaag
     2641 gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg caagtaagtg
     2701 agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca aggcactaag
     2761 tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg atcaaatcct
     2821 gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag gaattgttca
     2881 ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa atttggtgtg
     2941 ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa ttcgatcaac
     3001 aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat actttcgatt
     3061 cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac aaaccctgat
     3121 tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa aatcattcct
     3181 actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt ccatgcacac
     3241 agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt tgcttctaga
     3301 acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc
     3361 atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat atgcttgatg
     3421 tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc ggataatgga
     3481 gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag cgctggattt
     3541 aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat aacagtaaag
     3601 gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg gacaaaccac
     3661 tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc tgcttgtatc
     3721 catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat tcctggtttg
     3781 atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat cactgcatac
     3841 aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt taatccgaca
     3901 acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc taaaaaactt
     3961 ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat gttctaaaaa
     4021 agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt ataaaaataa
     4081 aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat taaaaacaga
     4141 tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta attcgtcaat
     4201 taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta accgctactg
     4261 cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca atgaaaaaga
     4321 aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa gtttattttt
     4381 tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg ttcttgcttg
     4441 attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc gagtagttct
     4501 gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt gaattcttct
     4561 gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt tggtatgact
     4621 tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt ttctatttct
     4681 tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa agaaggagta
     4741 acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt gcccgtactt
     4801 atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa tgtttgccga
     4861 tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt ggcagctact
     4921 cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa gcgtagagcg
     4981 ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt ttcaggtgct
     5041 gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt cgctaaagat
     5101 tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc gacatcctca
     5161 aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc ttcttcccct
     5221 ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt cactatgctg
     5281 gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc aatacctggt
     5341 aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga aatataacgt
     5401 gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct ttctttttct
     5461 aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc aggatgttcg
     5521 tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag atccttcaat
     5581 tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca tttttcttat
     5641 tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt ctcgattact
     5701 ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt attgttttca
     5761 tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc cactggttct
     5821 aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc gctattgatc
     5881 actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa ttcagaaatg
     5941 tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta
     6001 tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc atttgtaaca
     6061 tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt caaagtgtta
     6121 gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc agttccgtgt
     6181 tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc aatcgatcca
     6241 tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc caaattaatg
     6301 ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc tccaataatc
     6361 cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg gctacaccct
     6421 gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt tttcattttt
     6481 tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa attgacttaa
     6541 aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt ggaatcgatt
     6601 ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc cagccatggt
     6661 agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac caggcaaacc
     6721 ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg cttctgccgc
     6781 ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag caaaaaacgg
     6841 aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa
     6901 gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg aacgaatgat
     6961 attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc cattagggcg
     7021 tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga atgctgtcca
     7081 aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac
     7141 attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg atcactttga
     7201 tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat ttgatcaaat
     7261 aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc aaactcccta
     7321 acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt acttgatatt
     7381 tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa cttaattcca
     7441 ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg
     7501 cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct tcaggattgc
     7561 tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata accgctacct
     7621 tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt gttaaaccta
     7681 actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg
     7741 cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct cgtgcgattg
     7801 ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct ttttcttcca
     7861 aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc gattttccag
     7921 aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt agattgaaat
     7981 gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga gagagctgac
     8041 tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc aaattcatca
     8101 cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa acatcgcctt
     8161 tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt tgtttgaaac
     8221 atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct
     8281 caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc acctcatgtt
     8341 caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca agatatcggt
     8401 cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc cattttccaa
     8461 tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc ttctatcgta
     8521 cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac ataaactatg
     8581 tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct tccctcaagc
     8641 ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt ccgaatttgc
     8701 ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt gactcttctc
     8761 accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac gaaatcgata
     8821 ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg aaattcaaat
     8881 tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac agttcgaaga
     8941 tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata atttcggaca
     9001 aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc tgttgctcct
     9061 actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg ttccaaagct
     9121 gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg
     9181 tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt cgtaacagtg
     9241 gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat ttcttttgca
     9301 tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc ttcacgcttc
     9361 gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat atcaaaactt
     9421 tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg gatatcaaat
     9481 tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac taacaaacca
     9541 tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac aatacacata
     9601 gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc gtttataaga
     9661 gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc agataagcga
     9721 tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag cctaattcag
     9781 caccaaaact tgtaaaagga aggacagaac cgacaataat tcctaatgta gtgataacaa
     9841 acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc agtgcatgga
     9901 gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat aatgcgatga
     9961 aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca gcaggacaaa
    10021 taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt
    10081 aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc aaatattctt
    10141 tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca
    10201 aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa
    10261 acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata ttcccaaaag
    10321 tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct aaaaggatca
    10381 cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg atatcagcca
    10441 ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta tgaccatttt
    10501 tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg aatacttcat
    10561 ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc tcaccggtaa
    10621 ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact aattcgttgt
    10681 ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac gcttgtttcg
    10741 ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg tctgactcat
    10801 ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca ccaatcactc
    10861 gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt cgttcttttg
    10921 ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc atttcttcaa
    10981 tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca acactcatac
    11041 gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc ttatctgtat
    11101 ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc agccctgttt
    11161 gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt ccttcacaat
    11221 ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta tccgtacata
    11281 acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact gttccttttt
    11341 tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga agcatttcag
    11401 gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa tccccttttg
    11461 taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg atcagtagca
    11521 tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca gatagcgttt
    11581 ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct tccgcacttc
    11641 cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg ctttgctgat
    11701 cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc aatgctgctt
    11761 gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc atatcccctg
    11821 ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct gtgtactttc
    11881 cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca gccgcttgat
    11941 tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata gtgaagataa
    12001 tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc gtgatgaatg
    12061 aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct ttcaatattt
    12121 ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg
    12181 aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt tcaaataatt
    12241 cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc tttttttcca
    12301 tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg tctttagcta
    12361 ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat aatagccctg
    12421 gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct catctccttt
    12481 actaagtaat tttaattcat gacttccaat catttttcga atagttattt ttccaattag
    12541 accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa cctctcgcca
    12601 aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg
    12661 agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct tatgaaaagc
    12721 cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg ggcagtagcc
    12781 tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg ttaatataac
    12841 aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt ttaaaataca
    12901 cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc ccgctataat
    12961 agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt atcaagccag
    13021 acggcgtgag acgccattta gtcggaagca tcatccaacg ttttgaagca aagggactgg
    13081 ccattgctga aatgaagttt gaaactatga ctcctgaatt agcaaaagaa cattatcagc
    13141 acttgactga gcgcagtttt tttgatgaac tgatcgatta catgacttcc ggtcctgtcg
    13201 tttatcttgt gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa
    13261 caaaagctgc cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg ccggggactg
    13321 aaaacatcat tcacgcttct gattcccgtg atgctgcggt aaaagagatc gcccgctttt
    13381 tcccgccatt cgatataaac aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa
    13441 ctgctggtct agttgctggg ttcagcaatg gccagcagtt ttttctctaa ggacctccgt
    13501 tgattctttc ctaacatata ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt
    13561 tcttccccta aagcagcaga aatcaatctt tgataataat ctgccatcaa tgtttcatag
    13621 atcactgtac ttctttcttt atgtgataag ctccgtttca tatcttcatt cacactacgt
    13681 gcctttttca accgaccatt ttctatataa aacgaagtca ggttcattaa aaaagtaaag
    13741 agatactgct catgattgtt caactgtgct aatcgtcgca ttcttttttt gattttcaaa
    13801 taattgttct caatgaaccg atcactgaat atccaaggaa aatcagctag aatagatagt
    13861 tcaaattgac catagtctgg tatctgcatc aagtattctt caatgattcg gcattcataa
    13921 cgtaaaaatt caaattctgt ctcagatact tctgtttttt cgaccatgtg aagattcaaa
    13981 accaaataat acagatcttc tgttttctta aaatgcctac ggttattgct gatctcttta
    14041 taaggatcaa agctgtcttc ttgtacccga tctagcattc gtcttttttt ctttaacttt
    14101 attggttcat ccagctggtt tcgcatccat tcatattctg caagtgtcat gttcattcgc
    14161 tctaaaaatg atctcaatgt tacaaatgac atttttttca ctgaatttgt ctcaatcccg
    14221 actagagaag tcttaaaaca gattccttcc gctaattggg actgtgtcaa cttcttgttg
    14281 actcgaagtt tcttgatcaa ttcaccatca tacataatag ttctcctttt taatttaaat
    14341 ttttccacat tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa aaataaaaaa
    14401 atgttttttt cataaaaaat accaaccttc aagcattaga atgttgtcta actactgaag
    14461 gttggtttga ttgtttattg attatttttt tgctgttcaa cgtctgttct tgttactgga
    14521 tataagttgg ttgttgtttt attaccattt tttgaaaaga cacttgtcga atgttcgcct
    14581 ttttgctttt cgattgcttc attacgttcc gcttcatttt tatagtcata ttttttcgga
    14641 tcaatcgtct caaatccgtt tggcacataa aaccgcaaga gattttcctg attcagctta
    14701 tctgaaagac ttaacgcagt cttgactttt tcctgatctt tggagatttc ctcatcgatt
    14761 cctgctgctt tcgtatcgat gatttctcct gtctgattat tgtaagcttt tcctcctagg
    14821 acggtgtatt tgggtgtcac aaagttccca tttcgaaaag caactacttg atcatgctga
    14881 gaagataata agtctgtacc aaaatgaaga taatctttat catcgattcc caatagatgt
    14941 agtaatgtcg gcaaaacatc gatttctcca ccgtattcat gattgactgt ccctttagta
    15001 taaccaggca tatggatcat caatggcact cgttgcatct gtgcgttgtc aaattcatcc
    15061 catgtatctg gatctttacc aagagcgctt gctaaatctt tattatctgt attcgagatc
    15121 ccaaaatgat ccccgtaaat cacaaagatt gaattttggt aaataccaga ttttttcaaa
    15181 tacgtaaaaa attgttccaa tgattgatcc agataatgtg ccgttcgaac ataattatct
    15241 actgtgctat tgccagtatt caaagatgga aagtcgaaat tcttgtcatc cgtataataa
    15301 ggcgtgtggt tggttacgga aagaaatttc gtgtaaaacg gctgttgtag atgctcaaga
    15361 tattgggcag attctctgaa taaatcttta tctaatatcc cataacctaa cgtttcgtcg
    15421 gattcatcaa aataagatcg atcaaagaaa ttgtcataac ctaagttttt atatacatga
    15481 tctctattcc aaaaactagc tacattccca tggaaaacag cgcttgtata gccttgcttt
    15541 tgtccaagga tcgctggtgc ggcttgaaag acattgtctg acccaagttc tgtgaataat
    15601 gagccttgag gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc acttgtcttt
    15661 ccctgtccta cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt ctggaaaata
    15721 ctatttaaaa acggtgtgac ttcttgtccg tctaccttca tattaatcaa aaactgctgg
    15781 aaactttcta aatggagaac gatcacattt ttccctttag cgatcccaaa tgtttcggga
    15841 tttggctctg cataatgttt ctttgtataa tctaaaactt catcaatccc attactgctt
    15901 gcatgcgcgc gaacactgct agtcattcct gttttgattc cgtcataaat agtatacgca
    15961 tcgattccta gatatttgac aatatatgaa cggtcaaatg ttcgctgcag caattgtgga
    16021 cggttggctt cgctcagcgc tagatttcct gaaaaaataa gccctgaaag acaggtaaca
    16081 gcgatagcca tcggtttgcc gacagggttg cttttgatag gtattttctt gaccttcaac
    16141 caaaccagta gcccaatgaa cacagcgata tccagccagt agatgacatc atggggcttc
    16201 atcaaagaaa aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc taatacagat
    16261 ttgactgtaa tgaaatctgt gaattcccga taaaaaataa tattcaagta caacaaaagc
    16321 gtatttaaaa tatcaatgat caaaagaata gctaacgctg gctttggctt tttaatatac
    16381 aaagaaaggc caaaaaacaa taaggtagtc gcaattgggt tgatccacaa gataaagtac
    16441 tggatcgtac cttccacacc taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc
    16501 cagaaaaaga aaacagctgt caaaacgatt gctaaacgat tttggcacaa ttttttaagt
    16561 tgtgtcacgt atatcacttc tctctattca cattttttct tattttgctc ctgaaaaatg
    16621 aaaatttcct aaaaaagttg ttatcttaag atttttttat tctttctaaa gattattccg
    16681 tccacttttc acagcataaa accgctgcga tgtaagcgat taaactaatg aagagataaa
    16741 agtcttgtgg tgaccgcttc actccacggt attcaaaagc cagtttctcg gtattcgtac
    16801 aaaaaaagag gctgtgacaa aattcttgtc gcagctcctt atttcttgat gcaggacagc
    16861 tttttcacaa cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc acaagtcaca
    16921 atatttataa aacatgagaa gctgtttttt gaaattgttc cttgtggctc agagttaaac
    16981 ggcaggctca agacctcttg atacacggtg ttcaatcagc tgaccctcgg tattagatta
    17041 tgtcaggacg ctaattttac aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg
    17101 taataagccc aaccaaacga aaaatataag gagctatttt ttgaaattgc tccttgtggc
    17161 tcagagttaa acggcaggct caagacctct tgatacacgg tgttcaatca gctgaccttc
    17221 ggtattagct caaaaaacgg caaaatatga ggaactattt tgccgtttgt ttctctaata
    17281 ctcaggtcaa aacgctgatt tcacaacctc tgtttttcat tgtccatagg attgatattg
    17341 tccttgtgat gactcattcg taataattga ggcttgattt tcttcacttc ccgttatcgt
    17401 ttgtccactg ccagaatctg aaggacttgt tcctgtcgac tcgttttttt gagaagagac
    17461 agttgttttc ttgacttgtt catttgtctt tttccattcc gctaaacgat cggcgaccac
    17521 ttttttgtct actttttcag aataagcggt gatcactttt ccatttttca aacgggctac
    17581 tttgtcgcca tcatgagtca aaagcaaaac ggtcaaatgc ttgtctttaa attgatattg
    17641 tctgagagaa atcgcactaa gtacgtcatc gatattaata aattgatttt cttcttttcc
    17701 ttttggcaaa taaatgtcca attcgccttt ttcgtaattg gcagttattt tatactccgc
    17761 ccaccaataa gttgagaatt cttttttgaa attatctact gattctttgc ttgctacgat
    17821 tttttctgtt tctttctgag caagatctgc tgtcattttt cctgaaaatg ctgcggataa
    17881 aataagcagt aagaaaaaac cgaataacca aaagagaaga aacggtcttt ttttcctcat
    17941 gctttcctcc actcctattt aattttgact attctattat atacaaaaaa tgaaatagaa
    18001 agacaaaaaa gttgaaaaag atcgtaaata attgaacttc tttttcacaa aatggtaaga
    18061 atggagaatt tcacgcattt gctctcaact attctttctt aagcctcaaa tccgttttta
    18121 tctgatacgt ctttgaacca atgacccgat tttttgattg ttctttttcc atcttgtgct
    18181 aagtctactg aaataaagcc atatcgattc ttatatgcat tggtccacga ccagttatcc
    18241 atacatgtcc acatatgata gcctaaacaa ttggcacctt cttgaatggc ttgatggaca
    18301 tatttcagat gagattggac aaattcgatc cgataatcat cgtggatcat gccatcttct
    18361 ttgataaatc gtgcttcgtt ttctacaccc ataccgtttt cagaaataaa gcagtcgata
    18421 tttccataat gatctcgtac attgatcaaa atatcataga ttccttgttc atagatctcc
    18481 catccgcgat aaggattcat tttacgacca ggcatttcat aattgtcgaa ataatcctca
    18541 ggcattggac cgtttttagt atcgattggt gtttcttttg cttttacccg gcgtggctgg
    18601 taataattga tacctaaaag atctactgta ttttctttga taaccgtcaa atcctctgca
    18661 tggatcacag gcatgagatc aagttctttt actatctcaa tcaattcact tggaaactcg
    18721 ccttttacag ctggatcaag gaatgaacga ttgaagaacg catctgctat ttgcgctgct
    18781 ttaacatctt cagggtttga ttcgtctctt ggatagcttg gtgtcagatt caagatgata
    18841 ccaataacac caccttgctc catttcatgg taagatttga tggctaacgc tgaagccaac
    18901 gcttcatgat agccaacttg gatcgctttt ttcatatctg actcttctgg ataatgccat
    18961 cgatataaat aacctgcttc gactggaacg attggttcat tatgagtaaa ccattttttt
    19021 actttcgaac caaacaattc aaaacaactt ttggcatatt tttcataggc ttcaaccgtt
    19081 tctcggttca accatccgcc tttttcttgc agatacatcg gcatatcgaa atgatacaga
    19141 ttcacaaagg gttcgatacc gttttcaatc aactcatcaa aataacgatg atagaaacga
    19201 acggcttctt cgttgattgt tccagattca tcaggtatca aacggctcca ttggatagat
    19261 gttcgaaatg aattatgccc agtctgtttc atcaactgta catcctcttg atacttcgta
    19321 taaacttgcg aggttttttc aggtcctact tgatcaaaga acttttgcgg ttcttttttg
    19381 taccaataat caaaaatgtt ctctcctttt ccatcgcctg aaatccttcc ttctgtttgt
    19441 gggccacttg ctgcagaacc ccaccaaaaa tttgctggaa attgatattt tttcatcaaa
    19501 gcacctcttt ttctttttta gttcgtttta gttgattcca tgtatgatat tttccatcac
    19561 aatcactttt tcattttctt cctcttcttt tttgattcgt tttaacagct gttctgctgc
    19621 tcgttccccg attttatctg ttggtggcgt gatcgtgatt ttcggaagga tactcagatc
    19681 gtttttatct aaataatcat agtgactgaa cgcaatatcc ttcccgattc tgacaccttc
    19741 ttgattgaaa cattcgatgg caccaatcga catattgtaa ttgctgacaa aaatagcagt
    19801 tgcccctttt tctaataatg cttttgcccc gtaatagccg ctttttctag aatagtctcc
    19861 tgtaaagacc tctaattgga tcgatggatc attttggacc gcttctttgg ctcctgctaa
    19921 acgttctctt gctacgtagt ctgtttgagt ggcagcaata aatccgattt ttttatgtcc
    19981 ttgttcacca tgatgagcga tcacttttgc tacactttct cgatcattca ctaatattgt
    20041 atcaatacct gctggtcctt ctggacaatt caatgcgata actggaatac tcatttttgt
    20101 gagcatttca aatccagacc actcttcttc ggataagaag acaatcagcc catcgatcac
    20161 atgcttcatc aggtattcaa ttttttgttc aattttttct ggctgatcgg caaacccgct
    20221 taataataag ctatacccat tttcttcagc tactctttcg atccctgaga caacttccga
    20281 cccaaaacgg ctggaaatcc cgtttatcaa aagtccaata gagaagcttc gattattctt
    20341 caacccttta gcaatgatgt tttctttata gtccaacgct gaaatcgctt ctgctatttt
    20401 tttcatgttc ttttccttca gttgctgacc attcaagtaa cgggaaactg ttcccactga
    20461 aacaccagcc atcttagcca catcacggat cgatgccata cattctcact cctgccagtt
    20521 acatttgatt tctctttctt gttgttcgat tatgatgacc gcattctttg catagtgttg
    20581 cttgtcatca atatccatat aactaaagtc ttcagtagag caacttcctc tcactattaa
    20641 ttatacttaa acaagtattt gaaccggttc atttttaata tagcattttt ttaaaagatt
    20701 gcaacacttt tttaaaacgc tttctgaaag tggcggattt tttgcctttt taaaatttta
    20761 ttctaaaatt tactctattt gtgggtgttt tgtgcgtttt tctcttccat ccgtttgaag
    20821 ttttacattc caatggtata atttctttat cctatcaaat gggggaagta ttttgaagaa
    20881 attaatcgta ctatttattt tgtctgcggg acttttgaca ttgactggct gtgatttgtt
    20941 tggcggctct gaccaatcct cggacgaaac aaccgattct tctgtcttaa aagcggaaaa
    21001 agcagatacc accaacagtg tatatacgct aaaagtacgt aaagatatgg atttatccgc
    21061 gggacagttg acatttacag ataaaaaaat ggaatggaca cgcacgtatc agacacaagg
    21121 aactgattct tctgaatcat ctgaagaaaa aacagtgctg acagatatca agatcactac
    21181 caaagatgac gaatatatta tttcaggaaa agaaaataaa aaagaaacaa cagtcatatt
    21241 tacaaaaatc ggtaattacc gtttgaagga tgaggatggc aacatctaca gtctatagtg
    21301 ccgtctgttg gatcttcttt tgtttctccc cttcttttct gctatagtaa agaagcaaga
    21361 tgattgtaaa ggagacgaaa tggatgatcg atagtcctga ttattgtaca tgtaataaca
    21421 acaaaaatat ccatacagaa tttaatgacc aaggaagctt gagcatatgc gacaactgtc
    21481 acaaactagt caaagattca tttaaaacat ttgaatctta tcctgttcaa aattatgtaa
    21541 aaagtagaag aagataaaca aatccaagca aaactcacta tggctgattt ttatgaactt
    21601 caaactcagc ttcttccttt gatcaagctt catcagtaaa acaaacagtg gatcatttca
    21661 gtttacaaat caaagagtaa agatttattt atttcaaata tttgaatata aaataaccaa
    21721 acgatagaaa ggcttattct acatcacgta gtaatttttc tttcccgtcg tttggttttt
    21781 tatttttagc ctaattcttg accttttgca tgctggttat ccgtaaaata ttttccagca
    21841 ggctttcctt ccaattcagc taatttttct tggatctgac ggatcacctt ttttgcatct
    21901 tctggatttt ctacaaaatc gaatttatcc ccatccactt gtattttagg acatacatca
    21961 aaatcttcat accattgatt atagcgggtg ttcaacgttt cataataggt atataacgtc
    22021 tcatcgcttt ctagctgttc atagtcacgc ccacgttttt tgatacgttc tagcatcgtg
    22081 tccaaagaaa ctttgatatg gaccatcaaa tcaggccgtt tttttggagc aacatcatct
    22141 aactcgttta gcatcgtatc taataagcta tcgtactgct gtacttcgat atccgttacc
    22201 cgacctaaat cagcgtttag atggaacaac aggctatctt catagatgga tcggtctagc
    22261 acattgtcat cgttcactaa agcatttttc attgctaaaa atcgcttgtt caaaaagaag
    22321 atttgcaata aaaaagcgta ctgctcagga tttgaataaa agagcggcaa tacttcgtta
    22381 tcttcgacat tttcataaaa tggtttagaa tcaatctctt ttgcaagcat atccgtcaaa
    22441 ctgctcttac ctgcaccaat cgttcctgaa atcaaaatca ctgacataca acccttcctc
    22501 ttctataagt actcgcctac gtaagtttca acaggcaatg tttcaaaatc atattcacgt
    22561 acagcttggt ctgattttaa atacgtatgg agactttgtg taatcatcaa gttcgtaggt
    22621 accgtctctt cttgtacaat gatcatcggt tttttcaaca tagttgccac acctaattcg
    22681 taagccgttc ctggatcgat tgtttgatgc tcaaagtcaa ggatagcaac aacaaattct
    22741 gcatttgtca gttcttccat atctttttcg tatacttcct gcgcccatcc agcgctgaat
    22801 agttcataat tgctttcttg gtgatgacgt ggagagtaga aacttgtgac tgttttgttt
    22861 tcctccaacg ctttttcgat acgacttact cgatcgattt gctcctctga aaaaaatggt
    22921 cctgctaaat atatttgact cattattttg cctccttcaa taataactga tttttaatag
    22981 atactgctcg taattcattg accagttcca atggattgtt ggaaaagatt acttgatcca
    23041 accatttttt atccataaaa tcctcttttg caaaagtttc taataattgt tgcaagccgc
    23101 tgtaataacc gccaatattc aatattccga ttggcttgtc gggcgtcaga ccgattgcta
    23161 tccaagaaag aagttgtgag aattcctcca atgtacctag acctccaggg aaaacgagga
    23221 cagcatctcc cttttcaaat aataatacct ttctttcatc gatcgtttct gtaggaataa
    23281 atgtagtaac atcttttcca ggcaattcag tttcaaaaag cccattcgga tagatacctg
    23341 tgacttttcc gttattttcc agtactgttt tactaatcac tcccataatc ccggagtatg
    23401 aaccaccgta aaccagttcg atttcttctt gtgccatgta ttttcctaac gtttgcgcaa
    23461 taaatttgta actttcttta ttaccaaaac gggaaccgca aaaaacagta agtttcataa
    23521 aaaaacactc cctaactcaa aagtctatct tcattctaca cgtgtttttt taaaataaaa
    23581 gactagattt tgtagttttt tagtggtata tag
//
LOCUS       DACCXM010000037        23452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCXM010000037 DACCXM010000000
VERSION     DACCXM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.039"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            26..1687
                     /locus_tag="IVY61_001213"
     CDS             26..1687
                     /locus_tag="IVY61_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7970337.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1757..2119)
                     /locus_tag="IVY61_001214"
     CDS             complement(1757..2119)
                     /locus_tag="IVY61_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP7970338.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2187..3128
                     /locus_tag="IVY61_001215"
     CDS             2187..3128
                     /locus_tag="IVY61_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7970339.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3129..4172
                     /locus_tag="IVY61_001216"
     CDS             3129..4172
                     /locus_tag="IVY61_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7970340.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4178..5248
                     /locus_tag="IVY61_001217"
     CDS             4178..5248
                     /locus_tag="IVY61_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970341.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5238..6191
                     /locus_tag="IVY61_001218"
     CDS             5238..6191
                     /locus_tag="IVY61_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7970342.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6203..7213
                     /locus_tag="IVY61_001219"
     CDS             6203..7213
                     /locus_tag="IVY61_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7970343.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7498..8319)
                     /locus_tag="IVY61_001220"
     CDS             complement(7498..8319)
                     /locus_tag="IVY61_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7970344.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8416..8694)
                     /locus_tag="IVY61_001221"
     CDS             complement(8416..8694)
                     /locus_tag="IVY61_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970345.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8962..10971
                     /gene="metG"
                     /locus_tag="IVY61_001222"
     CDS             8962..10971
                     /gene="metG"
                     /locus_tag="IVY61_001222"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP7970346.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11450..12037)
                     /locus_tag="IVY61_001223"
     CDS             complement(11450..12037)
                     /locus_tag="IVY61_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970347.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12212..13534
                     /locus_tag="IVY61_001224"
     CDS             12212..13534
                     /locus_tag="IVY61_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7970348.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13689..14465
                     /locus_tag="IVY61_001225"
     CDS             13689..14465
                     /locus_tag="IVY61_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7970349.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14458..15027
                     /gene="rnmV"
                     /locus_tag="IVY61_001226"
     CDS             14458..15027
                     /gene="rnmV"
                     /locus_tag="IVY61_001226"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP7970350.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15020..15904
                     /gene="rsmA"
                     /locus_tag="IVY61_001227"
     CDS             15020..15904
                     /gene="rsmA"
                     /locus_tag="IVY61_001227"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP7970351.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16355..16984
                     /locus_tag="IVY61_001228"
     CDS             16355..16984
                     /locus_tag="IVY61_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970352.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17108..18217)
                     /gene="ugpC"
                     /locus_tag="IVY61_001229"
     CDS             complement(17108..18217)
                     /gene="ugpC"
                     /locus_tag="IVY61_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP7970353.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18426..18848
                     /gene="mgsA"
                     /locus_tag="IVY61_001230"
     CDS             18426..18848
                     /gene="mgsA"
                     /locus_tag="IVY61_001230"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP7970354.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19171..19707
                     /locus_tag="IVY61_001231"
     CDS             19171..19707
                     /locus_tag="IVY61_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7970355.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19747..21510)
                     /locus_tag="IVY61_001232"
     CDS             complement(19747..21510)
                     /locus_tag="IVY61_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970356.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21513..23228)
                     /locus_tag="IVY61_001233"
     CDS             complement(21513..23228)
                     /locus_tag="IVY61_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970357.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7549 a         4298 c         4774 g         6831 t
ORIGIN      
        1 aaattccaat aaaggggtac gtaaaatgaa aaagaaattt acatttggtt tggttgcggt
       61 atgcggcctt gtacttgctg gatgttatgg cggcagttca gccacaagca gtagttcagc
      121 aaaaagcggt tcagcagatg gaggaggcgt atttaactta gtcgtgcctc aagaaatgcc
      181 tacagctgac ttatcagttg caacagatac aatcagtttt acagcattga ataacgttta
      241 tgaaggaatt taccgtcttg atgaagacag caaaccacag ccggcaggag ctagcgagtt
      301 ggcagaagtc agtgaagacg gattaactta caaaatcaaa ttacgagaag acgcaaaatg
      361 gtcaaatggt gatcctgtta cagcagcgga ttacgtttat ggctggcaac gaacagtaga
      421 cccagctaca gcttctgaat atgcttacct gtatgcgcca gttgaaaatg cagaagacat
      481 tacagctgga aagaaagata aatcagaact agggatcaaa gcagttggtg actatgaatt
      541 agaaatcaaa ttgacgaaac aaacaccata tttccaatat ctgcttgctt tcccatcatt
      601 tttcccacaa aaacaatccg tagtggaaga aaatggggat gcctatgcat ctgcaagtga
      661 taaagctgtt tataatggac cattcacatt agaagatttt gatggaccag gaacagatac
      721 agaatggact tataagaaaa atgatcatta ctgggacaaa gatacagtaa aactttctga
      781 aatcaaagtc agtgtagtaa aagaatcttc tactgcattg aacttattca aagatggcca
      841 agcagatgac gtgatcttgt ctggtgagtt agcacagcaa aacgcaaacg atccagctta
      901 tacttctgtt aaagaagcgc ggacaagcta tatcgaattc aatcaacgtg aaaagaattc
      961 accattcaac aatgtgaacc taagaaaagc tatttcttat tctatcgatc gtgaagcttt
     1021 agtaaaacaa gtactaggtg acggatcagt tgtctctaca ggattgatcc catcagatat
     1081 ggtaaaaagt ccaacaacga acgaagactt tgcaaaagaa gctggaaaat tagtttctta
     1141 tgacaaagat aaagcaaaag aatattggga aaaagcgaaa aaagaattgg gcgttgattc
     1201 tttagaattt gatttgatgg cttctgatga tgattcatcg aaaaaagtaa tcgaatatgt
     1261 tcaaaactca atccaagaaa acttggatgg cgtaacagtc aaaccaacac ctgttccatt
     1321 ctctgtacgt cttgaccgct caacttcggg tgattttgac gcagtactag gcggatgggc
     1381 tgctgactat gcagacccaa gcagctttac tgatttgttt gtcacaggta attcatacaa
     1441 ccgtggtcgc tggagcaatg cagaatatga cgaagcagtt aaagcagctt catcaacaga
     1501 cgctggcgat gaacaagccc gttgggatga tttccaaaaa gcaatcaaga tcatctctga
     1561 tgatatgggt gtcgttccgg tgtaccaaaa agcagaaggt cacctagtga atgaaaaaat
     1621 caaaggtatc gttcaccatg cggcaggcgc ttcatgggat tacaaatgga catacgttga
     1681 agaataatcg ttaataccgt gagcatgaga aaaaacgagg ctgcggcaaa tgtcgcaacc
     1741 tcgttctcat attcacttat cctaacaaag aaaaaagcat gaaaatagca gaaagagcaa
     1801 ttagtattcc ggcaatgatc agccaaaagc tgtacatacc gtagctggca gacgagagcg
     1861 tggaaaattc tggttttttc tttcggttcc ttgttcgtgc aagatggacc gatcgctgaa
     1921 aatgatcgaa gaaacccgaa gaaaagaccc ataaaaaacc gccgataatc agaaacggca
     1981 atgaccataa gaatagatca ttagataatt gaatgagtgt aaaggaatgg ttgatgagat
     2041 tttttattaa aataaccaga atcagtagaa aagctactgc gtaggggata gatttttttt
     2101 catttgattg ctcctttatt tttggttcat tatctaataa tgtagcgaaa catctgtcag
     2161 aagtcaaaag ctagggggaa acattagtga atagttatat caaatatgta ttaaaacgtg
     2221 tctttttcat gatcatcacg ctttggttaa ttgcaacaat cacctttttc ttgatgcaat
     2281 tgctgccggg cacgccatat acgaatcagg aacgtttgag tcctgaaaca atcgagatgc
     2341 taaataaaca agtcggtttg gataaaccag ttatcgtaca atatgggatt tatctttcta
     2401 atttgcttca aggagatttt ggaatttcct tccaatttaa aaaccagcca gtcgcacatt
     2461 tgttagctgg aagaatcgga ccatccttgc agttagggtt acaggcaatt atctttggaa
     2521 cagtcctagg gacgatttta ggaacgatct ctgccatgaa gcaaaataca tgggcagata
     2581 catcttctac actcgtagcg atattaggtc gttccattcc aaactttgta tttgctgttt
     2641 tattacaata tatctttgca atcaaattac aagttttgcc aatcgcaaaa tgggatggct
     2701 ttgtgtacac aatcttacca acaattgcgt tggccatgtc gccgcttgct gactctgctc
     2761 ggtttatccg tacggaaatg gtggaagtac tgcatagtga ttatgtggaa ttagcaaaag
     2821 caaaaggatt gagccgttgg gaaatcgcct ttaaacatgg tcttcgaaat agtttgattc
     2881 cattgatgac tttattagga ccgttagctg tagcattgat gaccggatca ctggtggtag
     2941 aaaatatctt tgctattcct ggtatcgggg aacaattcgt taagtcaatc acaacaaatg
     3001 attatcctac gatcatggct gtaacgatcc tttactcatt tatgctgatt tttgttattt
     3061 tgatcgtgga tctattgtac ggtctagttg atccaagaat tcgtgtttca gaagggagcc
     3121 gaggataaat ggaaatgata ccaaagcgtt atgaaacagt tgcagatatc cctgcggatg
     3181 aatttcttcc attacaaaaa aataccgaag aagaacgtga acagattact gcaccttctt
     3241 tgaattttat ccaagattca tggcgccgtt taaagaaaaa caaagctgcc gttatttcaa
     3301 tggttttact attggtcatc attttgatct ctgtgatcac aatctttgtt tctcctcatg
     3361 atccaactgc ccaaaacgtg gcttatatca atttgcctcc tcgtattccg ggaatcaata
     3421 tcgatggatt gaatggaaaa gcaatggttg gtggtgaact tgttgataaa tatgctcaag
     3481 caaacgtccc ttcaaacgta aatttctttt taggaacaga cggtttggga cgtgatgtat
     3541 taagccgctt atttatggga atgagaatct cgttgctgat cgcatttatt gctgcattac
     3601 tagatgtaac gattggtgta gcttacgggt tggtttctgg tttgttaggc ggacgtgtcg
     3661 ataacgtgat gcaacgtttt ttggaaatct tatcaggaat tccaaacctc gttgtcatga
     3721 ttttgatgtt ggtcgttttt gaaccaggaa ttttttcaat cgtggctgct atggccatca
     3781 caaactggat accaatggca cgtatcgttc gtgcacaaac aatgaaatta aaagatcaag
     3841 aatatgtttt agctggattg acattaggtg aatcaagaat gaaaattgca ttcaaacata
     3901 ttttgccgaa tatctctagt gtcattatta ttcaaatgat gtttagtatt ccatctgcta
     3961 ttttcttcga agccttttta agctttatcg gtttaggtct tacgcctccg tcagcttcat
     4021 taggaacgat gttgagcgaa gggtacaaga cattcctgta tctgccttat ctattatgga
     4081 ttccggcagt aactttatcg gttatcatga ttggctttaa cttacttgct gatggattgc
     4141 gtgatgcctt tgatcctaaa atgaaagagt gaagacgatg acgaaaattt tagaagtgaa
     4201 agacttagaa atctcttttg atacgtatgc tggaaaggta cgagcgattc gcggtgtcaa
     4261 ttttcatttg aataaaggag aaacattggc gatcgttggg gaatctggaa gtggaaaatc
     4321 agtgacgacc agaagcatca tgcgcttgct gtcaagcaac gccaatatag atgctggtca
     4381 aatcctcttt aaaggtaacg atatcgttgg aaaatcagaa agagagatgc aaaaaatccg
     4441 tggtaaagaa atagcgatga ttttccaaga tccaatgacc tctttagatc cgacgatgcc
     4501 cattgggaag caagtcgctg agtcccttag aaagcataat aaagtttcaa aaaaagaggg
     4561 acaaaaagct gcattagaat tgcttaaatt agttggaata cccgaagctg agaaacgtat
     4621 caacagttat ccgcatcagt tctcaggagg tcagcgtcaa cggatcgtga tcgcgattgc
     4681 actggtatgt tatccggaaa tcctgattgc tgatgaacct acaacagcgc ttgatgtgac
     4741 tattcaagca cagattttgg agctgctgaa agaaattcag acaaaaatcg atacatcaat
     4801 tatctttatc actcatgacc ttggtgtagt agcgaatgtc gctgatcgtg tcgctgtcat
     4861 gtatggagga agaatcgttg aagttggaac atcggaagaa attttctaca acccgcaaca
     4921 tccctatact tggggactgc ttggttcaat gccgacatta gatagtgcga acgatcgatt
     4981 gtatgctatt cctggttctc caccggattt attgaacccg ccaaaaggag acgcattcta
     5041 tccaagaaat gaattcgcga tgaaaatcga tgcagaacaa gaaccaccat tttttgaact
     5101 atcaaaaaca cacaaggcag caacttggct acttgctccg caggcaccca aagtgacacc
     5161 gccagaagaa atccaacgtc gctgggctat ctttaaagag aaacaaaaac atacttacgg
     5221 tcaaggagga attgctgatg ccaaataaac tacttgaagt taaaggattg aaacagtatt
     5281 tcaatgtagg aaaaaagaat gaagtacatg ctgtaaatga catcagcttt cacatttatg
     5341 aaggagaaac attcggttta gttggagaat ctggaagcgg aaaatccact actggccgta
     5401 cgatcattcg cttgaatgaa cctactgatg gtgagatatt attcgacggt caagatataa
     5461 cgaagatcaa agataaaaaa ggtttgacga aattccggca tgatgttcag atgatctttc
     5521 aagatccata cgcttcgttg aatcctcgta tgaaagtcag agatatcatt gccgaaggaa
     5581 ttgatgttaa tggactagct aaatcatctg aagagcgtgc aaaaaaagtc gatgatctat
     5641 tgagaactgt tggattgaat ccaagccacg gtactcgtta tcctcatgaa ttttcaggtg
     5701 ggcaacgcca gcgtattggt attgcacgag cattggcagt aaaaccgcgt tttatcattt
     5761 gtgatgaacc aatttctgca ctagatgtgt cgatccaagc ccaagtggtc aatttgttgc
     5821 aagatctcca aaaagaacat caattgacct atctatttat tgcacatgat ttgtcgatgg
     5881 ttaaacatat tagtgaccga atcggtgtaa tgcataacgg cttgttatta gagatgggaa
     5941 caagtgatga aatctataat cacggtgttc atccttatac agaaagtttg ttatctgcta
     6001 ttcctctacc agatccagac catgagagaa aacgcagaag aatcaaatat caacctgaac
     6061 cagatgacgg acaagtacgt caacttcgtg aaatcgcacc agaacatttt gtctatgcaa
     6121 ctgagcagga agtcccatat tacgcaaaaa aactaaaacg acaaaaggaa tctcttctgg
     6181 ttgctaacta ggagggacat agatgctaaa agcttttaaa ttccgtatgt atccgacaga
     6241 agaacaaaaa cagcaattga ttcgtacttt tggatgtgct cgatttacct ataaccattt
     6301 gctgaaaaag cggcagaaaa gttggcaaca aacaggagta gccaattttt cgctcacacc
     6361 cgctacatta aaaaaagaat acccattctt aaaagaagta gacagtcttg ctttagcaaa
     6421 tgcacagctt aatttggatc gggcttttcg caattatttc aagggacgtg ccagcttccc
     6481 aaagcttaaa acgaaaaaaa gtatgtggca atcttatacg accaacaacc aaacccgtac
     6541 gatctatctg gaaaatggct acttaaagtt accaaaacaa aaagaactaa tcaagataaa
     6601 tcagcatcgg ccagttgaag gatcgattcg ctccgctacg atttctgctc gatataatga
     6661 agaattttat gtcgcactac tttgtgatgt ttcgccagta aaaaaagaat ctttagctaa
     6721 atggatcggg atcgcctatc atccgaagac attgatccaa acatctcgac ctctagaagt
     6781 aactttacca aaatttcacc agacagaaga aaagctgcaa catgcacagc ggaaattgaa
     6841 tgtgaaagtc cgctccgctc accatagaaa gatcaggtta gatcaggcaa gcaattatca
     6901 gaaacaaaag agaaaagtaa tggatcttta tctgaaacaa aagaaccagc gagaagatta
     6961 tcttgaacaa ttatctggga aattggtcaa acaatatgac tacttatttg tagaatcttt
     7021 tccaaaagaa gaggcacatg ctgatttttc tattcacgac tggcataaat taataacaaa
     7081 attgcgttat aagtcccaat ggtataataa aaaatttctt ttgatcaata ccgacggagc
     7141 agaagaaagc aattccgtaa gaaagagcca agtagtagaa aaaatgggca gacattcggt
     7201 tatcaaggga taaaaaatga atgaaactga taggagcgtt agggttagcc ttgttaataa
     7261 aaagtcttaa tgacttttgt ttctaggaat ttttcttttg aagaaaagct cactaattat
     7321 gacaaaccaa taaaacaata gcagaaaaag ttcgtacgag ctttttctgc tattgttaat
     7381 tttttattca gaaagtttac gaatacatca aaattctgaa acaaaaaacg taactcacaa
     7441 agagttgttt ttgcgttttt tcttgaatga ttaggtctga atcttttgct cacaacctta
     7501 ttcatggtgc cccggcttga gctgatagat ttttgaagga cggccgattc ctacaggacg
     7561 ttcaccgatc tcatcgaaat attggagcat agctttctta aaattggaat gatcgatcgt
     7621 tttataatcg atccctaaga atttagcaaa tacttttcga gcttccgtaa tcgtgaaatc
     7681 tttacctaaa acttgtagaa cttgcggctc gtgttccatt ttattgacta cacggttgaa
     7741 tgctttcaag atgatttgac tatggtcaaa agccaaagta tctttgccag aagactctcc
     7801 agtgactaaa tcaagcgaga tagccacttc tccgctagat aaatggatct tgttactatg
     7861 acgttctagt gtgaaccagc gtacttcctt ggcatcatct ccggcgatta gcggttcttc
     7921 tccaataaag gctaagtagc tgactgtgac gacccagcct cgcggatctc gattaggcgt
     7981 actgaatgta tgcagttgct caatattttc ttttgaaata accacaccag tttcttcctt
     8041 tgtttctctt aagacgcttt ctccagttga ctcatccttt tggacaaagc caccaggcaa
     8101 tgcccatgaa tttcgataag gatgcccttt tctttggatc aacaaaactt tcagctgatc
     8161 ttcttctttg ttatagcaca tcagtacgat atcgacagtc aaagagggct tgtcatattc
     8221 tggacgttcc tgtttttgat accagttaag aaattcttct tcacttgctt tatgttcata
     8281 atataatttt tcttcttctt tagaagcaaa acggttcatc tgaccacctc cattgacagt
     8341 aaaagtactt tttaccttat tgtagcagac tctgaaaaaa aaccaaggat ttacttgaaa
     8401 aatctaggtt atcatttaaa tatgaaaaat aaatggaagt atgagattga ataaaaagaa
     8461 aagcagataa aacggcaata ctttataccg ctcttcacga atggcaaaga agccacaaat
     8521 aacgatatat acgtcaatga taaaacgcaa aatccaagct gctgtcataa aactgttcaa
     8581 atgcccatgg aggttaaaat aaccatacat acgaatataa aaatagctga atattaaatg
     8641 tgctaatgaa agaaaagcaa tactaagtgt aaaaaaaaga tgtttcttct gcatgattgt
     8701 tccagtccct tactaagata gatagtcttt attataccac gtgtgagggg agaaataaaa
     8761 agccgttttt tagtagatat gaaacaaaac aaaaacccgc atttattcaa cctattcatc
     8821 cttttaaagg atagattgaa cgtagataga agtcgtttat ccagtctttt tcattttgaa
     8881 aaaaagctaa cagcatgctt tttttatggt atactagcaa ggaaacgaat tcgcatcatg
     8941 cgataaagga ggaagagccc cgtggcagaa aaggaaactt tttatatcac tacgccaatc
     9001 tattatccaa gcggcaaact tcatatcggc aactcgtata ctacgattgc atgtgacgca
     9061 attgctcgtt ataaacggtt gatgggcttt gatgtgtttt atctcactgg agtggatgaa
     9121 catggtcaaa aaatcgaaac aaaagcagaa gaactaggtg taaaaccgca agaatatgta
     9181 gataaaatgg cggctgatgt caagaaatta tggaaaacgc ttgatatcag ttatgataaa
     9241 ttcattcgca cgacagatga ttaccataaa gcagctgttc aaaaaatatt tgatcgttta
     9301 ttagaacagg gagatattta cttaggtgaa tatgaaggct ggtactctgt ttctgacgaa
     9361 gaattcttta cagaaacaca acttgcagaa gtgtacaaag atgacgaagg taacgtgatc
     9421 ggtggtaaag cgccaagtgg acacgaagta gaattggtaa aagaagaatc atacttcttc
     9481 cgcatgagca aatacgcaga ccgtctattg gaatactatg aagaacatcc cgaattcatc
     9541 cagccagaat ctcgtaaaaa cgaaatgatc aacaacttta tcaaaccagg attagaggat
     9601 cttgcagttt cacgaacaac ttttacttgg ggagtaccag tcaagagtga tccgaaacat
     9661 gtggtgtatg tatggatcga tgcattatcc aattatatta ctgcactagg atatggctcc
     9721 gaagatgaaa gtctattcga aaaatactgg cctgcagatg ttcagatggt cggtaaagaa
     9781 atcgttcgct ttcatacgat ctattggcca atcatgctga tggcccttga tttgcctcta
     9841 cctaaaaaag tatttgggca tggctggcta ttgatgaaag acggaaaaat gtctaaatca
     9901 aaaggaaatg tcgtttatcc tgaaatgctt gtggaacgtt atggtttaga tgcactccgt
     9961 tattatttgc ttcgtgctgt tccgtttggg tcagatggtg tctttacgcc agaagatttt
    10021 gtttcccgtg tcaattatga tctggcaaat gatctaggga acttattgaa tcgaacgatt
    10081 gctatgatca acaaatattg tgatggactc gttcctgact atgcctcact tgtcactcca
    10141 tttgatagtg aactgtctac gacggctgct aacgttgtcg gacgttacca tgatgcaatg
    10201 gaaaaaatgg aattcaatac agcattggct gaaatctggg tgctgatttc tcgtgcaaac
    10261 aaatacattg atgaaacaga accatgggta ttagcaaaag acgaagaaaa gaaagctgaa
    10321 ttgaatagtg tcatgatcca tttagctgaa tctttgcgta tcgcagcgat attacttcag
    10381 ccaatcatga cagaaacgcc agagaagatc ttcaatcaac taggattgga ctctgaaaca
    10441 atgaatcttg aaggattaca ctttggcgaa tttccttcag gaacaaaagt agttgcaaaa
    10501 ggtacaccaa tcttcccacg tttagatatg gaagaagaag ttgcttttat tcaagaaaaa
    10561 atgtctgaag gtactcaaac aaacgaagat acagtcaaat gggatccaga agaaacagaa
    10621 cttgtttcaa cgaaagaaaa acaaatcaaa tttgatgtat ttgaaaaagt agaactaaaa
    10681 gtagcagaag tcatcaactg tcaaaaagta gaaggggcag ataagttgct gcaattccga
    10741 ctagatgctg gagacagcca agaccgtcaa atcttgtcag gaatcgctga attttatcct
    10801 gatccaagcg aattgatcgg gaaaaaagtc gtcattgtag caaatctgaa accacgaaag
    10861 atgcgtggac aaatcagtca aggaatgatc ctttctgcag aagcaccaga tggctcttta
    10921 caagtaatcg aagcaccaaa atccatgcca aatggctcag aaatcgcata gaaattcaaa
    10981 gggggtatga cactagttgt gtcatacccc ctttttccga aatttgagaa gcaattttcg
    11041 taaattcctt cttattactt gaagccaatc atataataca gaaacagaga actatccaaa
    11101 ataaatccta gaaaaagaag tacgagcttc gctgattttt gttaatttag aataatacta
    11161 ataggaatcg taaaaacaga aaaaaagcat aatgataaag ggtcgggcag attttaattg
    11221 attcatctgg tactcttttt tattgtcctt acattcataa agtttgaatg caatgacttt
    11281 attacatcta tatttatatc taacagtagc gttgatgtgt taattataag ttctatcact
    11341 ttatgttcaa ttagtttgtg ctcttatatg tgtgattgaa aatatagata aaaaaacaat
    11401 catcggcttt gacttcgatg attgttttct ttattttgta aaaatcctat cacttgattt
    11461 tatggtaacc ttttattttt gtcttcaaac ggcttggtaa gagagcagta agcagtgcaa
    11521 atgggatcga aaagacatac cagaaaatcg tacgtccgat accataatgt tgatagagct
    11581 ttgtcaacat cacgtgtaac aagatcaacc ctaaaagcaa ggagccagct ataaaccaat
    11641 gattatccca atgcatgact tcactaagtg ccatcactcc aaaaggatag aaataaaaat
    11701 agatcccgac taaggtcatc cgccagttct ttgtaaatgt tccgatattc aataaaaaga
    11761 aaatcgctga aagcaaaata ccgataggcg gaaaaagccc aaccaaaaag gaatacaaca
    11821 tcccccacaa gatcatcaca ggacctttga tcaatgcaac gattgcgatg aacaaagtca
    11881 gcaatattag tttttgtgat aacgttgcaa cagtcattaa tccaaataaa attaatgtga
    11941 accaatgcca atgggactgc tgttgttctt tacgggaaaa atcgacccgg ttggataaat
    12001 aatctgctaa tttcggataa gaacgcttaa aatccataaa ctgttcctcc ttcgtggaaa
    12061 aggtaggttt agtatacgaa aaataaaaaa gaaatacatc cgtcttaaga gggagttttt
    12121 cactgaaagt ggagaaaaaa gaatggtaaa ttaaaatatt ctcagttata ctgaaaatta
    12181 taccaaattt caaagaagaa agaggatggg aatgaaaggc aaactaaaac gagaaatcaa
    12241 tctgtttggc gcattagcta ccgttatggg aaccgtgatc ggtgcaggcg tcttcttcaa
    12301 aactgctgct gttactgcta gtactcagtc tacaagtttg acattgctag cttggctttt
    12361 gggtggtttc ttaacgatct gtgctggatt gacagtggct gaattagcta cagcaatccc
    12421 ggaaactggt ggagctgtaa aatatattga agctgcgtat ggaaaattgc ccagcttttt
    12481 attaggatgg gcgcaaagct tgatttactt tcctgctaat attgctgcat tatcgattat
    12541 ctttgcgaca caattgacga acttattaca attatctacc gattatcttt tgttgattgc
    12601 agtgatcacc gctgtttctg tgacagggtt gaatttgtta gggacaaaag tcggtgcttc
    12661 tgtacaatca gtgacgttga ttgttaaatt aataccaatt gctgtgattg tcatatgggg
    12721 attattgacc ccaggacaag gaactgtcca gttatttcca atcgaagctg gaaaagatgt
    12781 tacttttgtc gaaggattga gcagtgcgtt gttagcaacg ttgtttgctt atgacggctg
    12841 gttaggtgtc ggtgcaatgg caggggaaat gaaacggccg gaaaaagatt tgcctaaagc
    12901 aattattctt ggtttgagtt ttgtaacagt cgtttatcta ttgatcaatt ttgttttttt
    12961 gaaaactttg ccgattgatc atcttgccgg taatttgaat gcagcttctg aagcatcaga
    13021 cgtcattttt ggaggaatcg gtggaaagct ggtcactatt gggatcttga tctcggtata
    13081 cggtgcattg aacggttata cactaactgg gatccgcgtg ccttatgcga tggctttaga
    13141 agacgagcta cctttcagta aacagctgac aaagctttca aagaagttta cggttcctta
    13201 tgttcctgcg gttttccagt tagcagtagc ttgtattatg atgagccttg gttctttcga
    13261 ctttttaaca gacatgttga tttttgtgat gtggctattc agtttactga tttttatcgg
    13321 tgtctttatt ttaagaaaaa aagcgccgga attgccacgt ccatataaag taccacttta
    13381 tccaatcgtt ccgattattg cgattctcgg tgctattttc attttaggta tgacgatgat
    13441 gacgcaaacg aaattagcat tgatcggtat aggtgttacc ttgataggta tcccagtcta
    13501 ttatcaaaaa aagaaaaaac gctcagaaga gtaaaatatt tcaacaacag acagaaaaag
    13561 tacgaactag gtcctctaga tcttcgtgct ttttctattt caataaaaca agttcttcta
    13621 ttttgatttt tgctacttct gcaagtagtg cttatgtgct aaagtaagaa tgaaaaggag
    13681 agataaacat gatatttgat tctcataccc atttaaatgc tgaacagttc aatgaagata
    13741 ttccagaaac gattcagcga gcacaagaaa tgggggtaac gaaaatggcg gtcgtgggct
    13801 tcgatacgcc aacgattgaa aagtctttgc aattaagcca tgactatcca aatatataca
    13861 gtattatcgg ctggcatccg acagaggctg gcagttatac gaaagaaatc gaaaataaat
    13921 tacaagaaca attgacttta ccaaaggttg tagcattagg ggaaattggc ttggattatc
    13981 attggatgga agacccgaaa gaagtccaag ataaagtatt tcgcagacag attgctatcg
    14041 caaaagaaat gaacctacca atcagtattc atactagaga agcacttgaa gacacgtatc
    14101 aaatcctaaa agaagaagat gttcgagata tcggtggaat catgcatagt ttcagtggtg
    14161 attatgaatg ggcaaaacga tttcttgact tagggatgca tatttcattt agcggtgtcg
    14221 tcactttcaa aaaagcatta gacgtccaag aggctgcagc aaatgtacca atcgaacgat
    14281 tacttgtgga aacagatgcc ccttatctgg caccagttcc ttttagagga aagcgaaatg
    14341 aaccaggcta tactcgttat acagtagaaa agattgctga acttcggaat cttccgacag
    14401 aagaagtggc tgcccaaaca tggacaaatg cgcaccgatt atttggtcta agcgaacatg
    14461 gataagatga agatccaaga gattatagtc gttgaaggaa aagacgatac aagaaggctg
    14521 cgggaagtag ttgatgcaga tacgatagaa acaatagggt ctgcaatcaa tgaagagatc
    14581 ctgatgcaaa ttgaacatgc ccaagagact cgtggagtga tcgtttttac cgatcctgat
    14641 ttttcaggag aaaaaataag aaaaatcatt atggaagctg ttcctcaggc aaaacatgct
    14701 tttttgccgc gcacacaagc taaaccaaag aaaaagggaa gccttggtgt cgagcatgca
    14761 agtgatgctg ctattttaga agcattgaaa aaggtcgtga cgccagacat ttcccaaacg
    14821 gctgtttctg aaatcactag acaagacctt ttagactatg gtttaatagc tggggcacaa
    14881 gcaaaagaaa aacgagaaaa actaggtgac gagttgcgca tcggttacac gaacggcaaa
    14941 caattggaaa aacgtttgaa gatgtttcgt attacgccga aagaactcgc ccaagcaatg
    15001 aaaatagtgg aggaacaaca tgagtgaata tcgtgaaatt gcgacaccct ctagaacaaa
    15061 agagatttta ttgaaacatg gtttttcatt taaaaaaagt ttaggacaaa attttctgac
    15121 tgaaccaaat attttaagga aaatcgtaga aactgcgcaa atagatgaac atacgaacgt
    15181 gatcgaagtt ggaccaggaa tcggtgcatt gacggaacaa ttagccaagc atgcaaaaca
    15241 agtcgtagca tttgagatcg atgatcgttt gatccctgta ttggctgata cgatgcaacc
    15301 ctatgacaat gtgactattg tacatcaaga tattttaaag accgatttgt caacagctgt
    15361 tagagaaaca tttcatgagg agcttccgtt aaaagtcgta gcgaatttac cttactatat
    15421 tacgacaccg atcatgatgc attttcttga atcagatttg gttgtggatg aactagtcgt
    15481 catgatgcaa aaagaagtag ctgatcgtat ctctgctgaa ccaggaacga aagcttatgg
    15541 ttcattatcg attgctgttc aatattatat ggaagccagt ttagcattca tcgtaccaaa
    15601 aacagtattt gtccctcagc caaatgtaga ttcagcgatt ttgaaactga caagaagaga
    15661 tataccggca gtcgaagtga cagacgaaaa agcatttttc cgattgacaa aagcggcatt
    15721 tcagcaaaga agaaaaacgc tttggaataa cttgcagcat tcgtatggca aagatgacca
    15781 gacaaaagca tggttagcaa aaagcttgga aacagctgga atcgatccaa aacgtcgtgg
    15841 ggagactttg tcattacaag aattcgccgc attaagcaat gcgatgtcag aaaatatgca
    15901 gtaaaaaaag aggttgtgac agaagttgtg aaaaagctgt cctgcatcaa gtaataagaa
    15961 cagccaaaca aaaatagttc ctcatgtttt tcgtttgttt gggcttatta ccgcagatgc
    16021 aagcttttga acaccgttta agaaagaggt tgtgggcctg ccgtttaact ctaagtcaca
    16081 aggaacaatt tcaaaaaaca gcttctcatg tttttgagtt agttggtctt attacttgat
    16141 gcaagctttt gaccactatt atgccgaaca aggagatgtg acacaaattt tgtcacatct
    16201 cctatttttg tttcttgatc aaaaaattct tatacttgat actcacactc attttgtatg
    16261 gatttagaaa agaatatgtg tcttgacagt tattagtaag tgagttatac taagtcgaca
    16321 tttatgtgtc aagacatatt aggagagaga aaaaatgaga aaaaattcag ttcgaaaact
    16381 aacgatttct gctttattga ttgggatggg ggtcattatc ccaatggtca tgcctaaaat
    16441 catgattcca ccagcttcct ttactttggc gtctcatgtg ccattattta ttgcgatgtt
    16501 ttttacacca ggagttgcag tggctgttgc gctaggtacg acatttggtt tctttttaac
    16561 aacgccggtt atcatcgcat tgcgagcatt gtcacatctt gtgtttgcgt tgattggggc
    16621 ttggtacttg caaaagcatc cgagtatcgt tttaaaaaat ggacagttta cttttgctaa
    16681 ttggcgtttc caagggttca attttgtgat cggtgtgatt cattctttag cagaaatgct
    16741 agtagtcagc atcttttact ttattggtaa tatgcctgaa acatattatt ctcaaggata
    16801 tttttataca gtattcatct tgatggggat tggtgggctt atccacagtt tagttgatta
    16861 taatattgct tattttatag caggaacact cagcaaatat tttgatatcc ccgtatttac
    16921 ggcagcaaaa aaagaaaatc ggaaagagtc gattcaaggg tacgcgaaga aaacgacata
    16981 ttagacatga tctctcaaaa aaaaaactct catctagtaa tggataataa atcctctaag
    17041 atgagagttt tttgtcctaa aaagagctgc accaacatct tctggcacag ctctaacagg
    17101 gggcaattta tggaagtttg atgacttgtt ctgtttcttt atcaaagaag tggcctttgc
    17161 tgatgttgaa ggcaaggtcg actatttcgc ctggtttatg gaaatctctt gcatctactt
    17221 ttgagatgaa ctcagtagaa ccagtttttg tgtaaagcat cgtttcagct ccaagtaatt
    17281 cggaaacgac aacttctgat ttcactacgg cttcaggtga agcttcaagg gctacttgtt
    17341 cactgtggat gtcttctgga cgaataccga agataacttc ttttccttca taaccttttt
    17401 caactaatac tttgtttttt ccttcaggaa tacgaagctt aagcccatgt ccatctgtga
    17461 taacaccatt tttcaatgtc acattaaaga agttcatggc aggagatcca ataaagccgg
    17521 caacgaacat attgacgggt gtatcataaa cttctttcgg tgtaccgatt tgttggataa
    17581 atccgtcttt catgataacg attcggtcag ccatagtcat tgcttctgtc tgatcgtgag
    17641 taacatagat ggtcgttgtt tcaagacgct ggtgaagttt ggcaatctca gcacgcatgg
    17701 ctacacgtaa tttagcatct aaatttgata atggctcatc cattaagaag acttttgcat
    17761 cccgaacgat tgcccgtccc aatgccacac gttggcgttg tccaccagat aaagcagctg
    17821 gttttcgttt caagtattct gtcagaccta agatctctgc tgcattgtcc acacgttttt
    17881 tgatgtctgc tttatcatat ttacgtaatt tcaagccaaa agccatatta tcaaaaacag
    17941 tcatatgagg atacaatgcg tagttttgga aaaccatggc aatatcacgg tctttaggtg
    18001 ccacgtcatt catgactttt ccgccgatat ttagttctcc ttcagagata tcttccagtc
    18061 ccgcgatcat tcgcaaagta gttgactttc cacaaccgga aggaccaaca aaaacgataa
    18121 attcacggtc tttgattgat agattaaaat cagttacaga ataattttct gcattgtcat
    18181 attttttata tacgtttttc agagccattt ctaccataat acgttccaca ctttcttttt
    18241 tagttttatt aactatttaa agtataaatg aaagcgttaa ctaacttcaa tgtcatgttg
    18301 aacaagaatt ttttcttttt tttgtcaatg tgctagtttt aagctttaat aaatatccat
    18361 aagaaataaa aactgataca ctggagtaaa taagtatgtt aacaatatca aaaggagtgt
    18421 ctcttatgaa aatcgctttg atcgctcatg accgaaaaaa acctatgatg gtgaaattag
    18481 caacagcgta taaaacaata ttggaaaaac atgaactttt tgctaccggg acgactgggc
    18541 agaggatcac ggaagcaacc ggcttgccga tccatcggtt taaatccgga ccgttaggcg
    18601 gggatcagca aattggagcc atgatttcgg aggatcagtt agatttggtg atttttcttc
    18661 gagacccgct ggctgcacag ccccatgaac cagatgtaac agcacttatc cgtttatctg
    18721 atgtctatga gattccgtta gctacgaata tcggaacagc tgaggtgcta ctaagaggac
    18781 tggaagccgg cttcctagat tttcgaagaa tcgtccatga aatagacaag catccacttt
    18841 cattttgatg gataagcgta tggaaaaata acgtttaatg ttcgcctata tgtagctata
    18901 aaagctaaac aaactgataa tatgcgatat aatacgaacg tattggataa tataaacaat
    18961 aatattagtt tttcgtattt gcagtaaatg tgaaaatatg ctatagtttc tgtgggaaaa
    19021 gatcccaaaa ataatacaga gtagaaaata aagcgttaag tgctaagagg atgggatgtt
    19081 gcctcttgga cgaaggacgt aatgttcgcg gttttatttt cgcattcgct gcatgatacg
    19141 atgtagcagc tcttaaagga gatgctaaaa atgaagaaac accggttaga cgcacgtatg
    19201 attgctatta tgggcttatt gattgcattg atggtcactt tgtcccgact ggtagcaatc
    19261 gagaccccat ttattaaaat cagtgtcaca tttattccac aagtgatcat gggcattctt
    19321 tttggtcctt tttggtcagg gataggcgca gttttagctg atttagtagg aatggccctc
    19381 ttctcgaaat ctgcattctt tattggattt acattgaatg catttattga aggagcaatc
    19441 tacggattct ttttctatcg aaaagagatc acttggaaaa atgccattct ggctacgctt
    19501 agtgtcacgc tgatcatcaa cttatttttg acaccattat ggcttgcgtt gatgtaccat
    19561 gtgccattgt tcagctgggt cgtttgggcg ccccgcctgt taaaaacagt aatatggtta
    19621 ccgattcaat caattgccat ttattacgta ggacgtagta ttccttacaa aaaaatttta
    19681 agaagtttag cgatccacgc taaataaaaa acaagcagtc ttcccatccc agaagactgc
    19741 ttgtttttat tgtgctgtga attgtgcttg atagagccgt cggtaataac catctttttg
    19801 aagcagctca tcatgggtgc ccatttcgac aatagctccc tgatccatca ctaatatctg
    19861 gtccgcattt ttgattgtgg acaggcgatg ggcaatcaca aaactggttt ttccttccat
    19921 cattgctaag aaggcttgtt ggattttttt ctctgtcaat gtatcgactg agctggtagc
    19981 ttcatctaag atcaacatag ggggattact gatcatcgtt cgagcaatcg tcaacagttg
    20041 gcgttgtcca tctgatatct ttaacccttg accgccaatc ttagtatcta atttttccgg
    20101 caaacgttct acaaattcat acatataaga ggcttttagg gcatcattga tttctgcgtc
    20161 acttgcttcc ggattcccaa aacgaagatt ttctcgcaat gtactgtcaa atagccaagt
    20221 atcttgtaac accattccaa aacttttacg taatgtatca cgttggatag agcggatgtc
    20281 atggtcatcg actgtaattg ttccttttgt tgtgtcataa aaacgcatca gcaaattaac
    20341 aagtgtggat tttccggccc ctgtttttcc tactatagca atggtttgac ctggataagc
    20401 agtaaaattg aaatcttcga tcaaacgctg cttggggtca taagaaaaag aaacatgatc
    20461 aaaagagact tttcctttga tatttttggg ttccaaggca taggcatctg gcaaatcagg
    20521 cttttcttct ggctgatcga tcaaatcaaa tgctcgttct agaccagaaa aagcggtttg
    20581 cagttgtgtc gtgattccag aaagttcgat aaatggttta gaaaactgac ttgagtagat
    20641 cgtaaagctg gaaatcacac caacagtgat tgacgaacca ctcttcaatg ccaataggcc
    20701 gccaactaaa ccaatcgata aataagcaag gtgatctaca aaacgagaag acgggtttgt
    20761 caaggaagaa gaaaattgtg cccgttgtcc tttttcatat agatcagcat ttagcagttc
    20821 aaattgctgt tgactagctt cttcttgctg aaaagctttc acgattttct ggttaccgat
    20881 tcgttcggtc acaaagcccg agatatcccc aataatcctt tgctgtctat cgaaatttgt
    20941 ttgtgaagca cgagcaacga tccaactcac aaggaaaata attggtgtag aacaaagaac
    21001 gaccaatgtc agaagtggac ttagacgcat cataaaaata aacgaaagaa tgatgattgc
    21061 cactccagaa aataattgat tgaaggccgc agaaattgca atggaaatat tgtccatatc
    21121 atttgtaaat cgactgacga tatttccgtg ggagttttga tcataataac gaagtggcaa
    21181 tcgattcaga tgagcaaatg catcttttct taattttgca acggataagt aagctaaccg
    21241 attgcttaac acttggatga gccattgact gactacgttg attaaaacga taccaccgaa
    21301 tagaataagt aaatggaaca gtcgattgaa gcggacctgt ccttctccaa tcatttgatc
    21361 gatactttga ccaatttgta acgtcatcac aactgttgca atcccattga tgattcctag
    21421 tatcacagct agccaaatgt cttttgcata ggcagtaagg taaggaatga atcggcggag
    21481 cgtctgcaag gataattttt ttactttcat aactattctt cctcctgtga tgcgacgatt
    21541 tcctgatatt ctgctgattg agcaagtaat tcttcatggg taccctgagc agcaatctta
    21601 ccttgatcca tcactaagat atgatgtgct tcttgaatag agcggacacg ttgggagata
    21661 ataatcactg tactgccaag ataagtctgt agcgccttac gcaaatccaa gtctgtttga
    21721 taatccaacg cacttaaaga gtcatctaaa atcagtaatg ctggcttggc aatcaaagct
    21781 cttgcaatcg tcagtcgctg tttttgtccg ccagagaaat ttttcccatt ttccatgact
    21841 tttgtatcta aaccatttgg aagctgacgg acaaattctt ctgcctgagc gattttcaga
    21901 gcttcccaac attctttttc tgtggcgtct ttcttacccc attgaaggtt ttcacgaata
    21961 gtcccggtaa atagtacaga agtttgggga acaagagcaa tttgctgacg gagatgatcc
    22021 agctgccaag aacgaacatc cattccgtcc attataactg taccggaact gacatcatag
    22081 aaacgcggaa tcaattgtgt caaagtcgtt ttcccgcttc cggttggacc aatgattcca
    22141 aggactgtat ttcgcttcag ctgaaatgta atattttgga gagaaagacc agaatcagga
    22201 gtataacgga agtcaacatg gtcaaaagcg ataattgctt cagaagaaaa atcgggagta
    22261 gtactgacct cagcttcaag aatcgaattt tctgtgtcta gcacctcttt cacccgattt
    22321 cctgaagctt cggcacgtgt gaagatcacg acgaggtttg aaacaacgat cagtgccaaa
    22381 agcatttgat tcatataatt gatcaaagca agaacttgtc cttgttgcaa gctaccaata
    22441 tttactttga tcccgccaac tgtcagcaaa cagatgatcc cgacattcat gatcaaggtt
    22501 gtagcaggtg ataatagggc agagatattg gaaacacgta aataattgtt cgctagatca
    22561 tttgtgcttt tatcgaactt ctctgtttct ttttttgtct gcgcaaatgc gcggatgaca
    22621 cgaacaccgc taagattctg actaacgtgt tcattgagct gatctagttt ttcttgcact
    22681 ttttggtaga gaggaacggt ttttttgatg ataaaaaata aaatgattga gaaaataggc
    22741 aataatgcta agaaaaacag acctacttcc cagtcgatca caaaagccat gaccactgac
    22801 ccgatactta aaaacggtgc tctgatcaat aaacgaatga ccattgctaa agctagctga
    22861 agctgattga tgtcgtttgt cattcgggtg atcaaggtat ctgttccaaa atgattcaac
    22921 tcgttgtgag agaaagtatt gatttttttg attagctgat tacgtaactc tgtaccaaat
    22981 ccttgtgaag ctactgaagc atagtattgg caaatgatag cacaaatcag accaatcaca
    23041 gacatagcga ccatccataa gcccatttca atgatttttc ctcggtcatt catttttagt
    23101 ccattatcga tcaaggaagc catcattaaa ggcaggacaa gttcgaagac agcttcaaga
    23161 aatttgaaga acgggcccaa aatgatttgc ttacgatagt cttttgcata acgtaataac
    23221 tgaatcatat gattcctcct attatttatt tgtccacaat tgaaaaaaca agtactacca
    23281 cattgtagct taggaaattt agaatgaaaa gagaacaagc cttttcataa atgaaagttt
    23341 tattatgaag aagctttaga aattgaatag actatttttt ttaaaaaaaa gaaaatgaga
    23401 aaacatgcca gtctcttcat tcataggaac gtctatttta cttaattttt ct
//
LOCUS       DACCXM010000038        22951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCXM010000038 DACCXM010000000
VERSION     DACCXM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.040"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            104..1069
                     /locus_tag="IVY61_001234"
     CDS             104..1069
                     /locus_tag="IVY61_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7970358.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1126..1356)
                     /locus_tag="IVY61_001235"
     CDS             complement(1126..1356)
                     /locus_tag="IVY61_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP7970359.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1442..2329)
                     /locus_tag="IVY61_001236"
     CDS             complement(1442..2329)
                     /locus_tag="IVY61_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP7970360.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2418..3641)
                     /locus_tag="IVY61_001237"
     CDS             complement(2418..3641)
                     /locus_tag="IVY61_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7970361.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3658..4737)
                     /locus_tag="IVY61_001238"
     CDS             complement(3658..4737)
                     /locus_tag="IVY61_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7970362.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(4868..6595)
                     /gene="ptsP"
                     /locus_tag="IVY61_001239"
     CDS             complement(4868..6595)
                     /gene="ptsP"
                     /locus_tag="IVY61_001239"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP7970363.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6595..6861)
                     /locus_tag="IVY61_001240"
     CDS             complement(6595..6861)
                     /locus_tag="IVY61_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP7970364.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7340..9574
                     /locus_tag="IVY61_001241"
     CDS             7340..9574
                     /locus_tag="IVY61_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7970365.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9722..10042
                     /locus_tag="IVY61_001242"
     CDS             9722..10042
                     /locus_tag="IVY61_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7970366.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10247..10471)
                     /locus_tag="IVY61_001243"
     CDS             complement(10247..10471)
                     /locus_tag="IVY61_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP7970367.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10669..12249)
                     /locus_tag="IVY61_001244"
     CDS             complement(10669..12249)
                     /locus_tag="IVY61_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP7970368.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12650..14026)
                     /locus_tag="IVY61_001245"
     CDS             complement(12650..14026)
                     /locus_tag="IVY61_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7970369.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14154..15272)
                     /locus_tag="IVY61_001246"
     CDS             complement(14154..15272)
                     /locus_tag="IVY61_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7970370.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15414..15848
                     /locus_tag="IVY61_001247"
     CDS             15414..15848
                     /locus_tag="IVY61_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7970371.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15926..16459)
                     /locus_tag="IVY61_001248"
     CDS             complement(15926..16459)
                     /locus_tag="IVY61_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP7970372.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16553..17731)
                     /gene="mutY"
                     /locus_tag="IVY61_001249"
     CDS             complement(16553..17731)
                     /gene="mutY"
                     /locus_tag="IVY61_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP7970373.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17724..18521)
                     /gene="recX"
                     /locus_tag="IVY61_001250"
     CDS             complement(17724..18521)
                     /gene="recX"
                     /locus_tag="IVY61_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP7970374.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18628..20010
                     /gene="rlmD"
                     /locus_tag="IVY61_001251"
     CDS             18628..20010
                     /gene="rlmD"
                     /locus_tag="IVY61_001251"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7970375.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20184..20762)
                     /locus_tag="IVY61_001252"
     CDS             complement(20184..20762)
                     /locus_tag="IVY61_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP7970376.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21084..21554
                     /locus_tag="IVY61_001253"
     CDS             21084..21554
                     /locus_tag="IVY61_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP7970377.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21662..22888)
                     /locus_tag="IVY61_001254"
     CDS             complement(21662..22888)
                     /locus_tag="IVY61_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP7970378.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         6807 a         4642 c         3954 g         7548 t
ORIGIN      
        1 atcaaagtat tttctgtgaa cagaactata gaattcccta aaacttgtcc attatttcaa
       61 aatttgttat agtcttttta ttcaaagcgt aaaaggagat cctatgttaa aagaaaaaat
      121 gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa gaccgagatg gtaaggtcat
      181 ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc agctattttc atcagaataa
      241 acagcattca aaaaaagtat tacgtatgat tcatcatata acagattttg atttcgtcgt
      301 agtagacacg gaattagatg ctttattgct cgagtgtcaa ttgatccaac attaccgtcc
      361 tttttataac cgacaaatga attatttcag taattataat tatgtacata ttaccaataa
      421 gggatttgta ttaacagata ctcctacggc tcgaacatac gggccttttc gtctctataa
      481 aaaaatgccg tctattttgc gtataatgga agaaacttat caaatgcctt ggttgtctga
      541 aattagtttg ttagctttgc gagtccagct tcctgaccta caggaaatga cttttgaaca
      601 gaaaaaaaag gaactccaag ggctgtttca aggccgaaat aaaaagctac tgacttactt
      661 gaaaaaaaga caacaacatt tcatctatca gttgaatttc gaaaaagccg gtatgcttca
      721 aaaagatatc gaattagtta cttattttat tagacggatc caggaacaaa agcagttttt
      781 aagaacacca tcgctcacct tcagcatgcc attagcagca gatgagagtc agaaaaagca
      841 ctacctcgtt tgttatggtc aacttgcaga aacaatgatt acctctggtg acgtttctcc
      901 tgacttctat tatgaaaaga aagaagctca tttatctttg aaacgtcaat tgtcaaaaga
      961 agaaatcgat cctgtacaaa tcttgatcag ttaccgcaaa aaattagaaa aagaacagtt
     1021 tgaaattgaa ctgttaaaca aaaaagaagc tgaaaagcaa ctaaattagt tgcttctcgg
     1081 cttcttttag gcatcttcaa cttcaaatgt caattatcct aaaatttatt ggattaagtc
     1141 atagatttcc atcgctacga tatcgatatt atcaaattcg tattgctgat gagtatctgt
     1201 ttcagttaat tcaaatgtat cggtcgcttt atcataagtt actgtacact tctctacgcc
     1261 ttctttttca aaacggcgaa cttgcagttc attatctgct gactcgacca tagcctccag
     1321 tcgttttatg attgcgacta attgtgaatg tgtcatcgga ggtcctcctt ttcttatttc
     1381 tgttatattg tatcaaaagc atagcctact tgcattgatt ttctctaaaa aaactcgttt
     1441 tttatgattt tttttctggt atttttcctt caggccacca aagtttgatc aaagcttggg
     1501 ttccgtcatt ctcaaaacct ttatctgcca actcttcata tagtttcaat gcttgggtag
     1561 ttgctggcaa tacaagatcc attttttctg cttcttctaa tgcaatcttt aaatctttga
     1621 taaaatgttt gacaaaaaaa ccaggtgtgt aatcttcttt taaaatacga ggagaataat
     1681 tgcttaaaga ccagttagct gcacttccac ctgaaagcgt ctcaatcact ttctttaaat
     1741 caagtccgtt tttttgtgca tagacaagca tttccgtcat tcctgtcatc gtacctgcaa
     1801 tcatgatctg attagccatt tttgtatgct gacctttccc agcagaacca tgcaatgtaa
     1861 atgtcgtacc aaattctttg aataaaggca gtacttgatc ataaacggct tcttctccac
     1921 cgaccatgat cgtcagtgta ccattctttg ctcctaagtc accaccggat accggtgcat
     1981 ctaacgcatc tgcgcctttt tcttttgctg tttttgcaat tttttctgcc aggcttggtg
     2041 tacttgtagt caaatcaact aaaatatgtc ctgagatatc tgcagaaaaa atccccgact
     2101 gtccatagta gatttcttct acatctttgg ggtaacctac cattgtaaag agaatattac
     2161 tttgttctgc taccgctttt ggtgtatctg cccagatagc tccttctgtt actaaaggat
     2221 cagcttttgc ttttgtccga ttatagacag ctacttcgtg tcctgcttct agtagatggc
     2281 gtgcgacagc acttcccatg acgcctgttc cgataaaacc aagtttcatt ttttcgacct
     2341 cctaaaaatc aaaaatcctt gcttctatgc tagtatacgc tagaaacaag gattttttca
     2401 taaaaaatgt ttgaatttta tcttttaaat gaatttaatt ttattttgat tcgttcaatc
     2461 gagtcttctt cttcttttct tcgcatctct tcttcatgat aaacaaccat gtcttgataa
     2521 aattcgatca tagcttcgcc gaaatgtgta gatgaaatat cataaagttt agcttctaaa
     2581 acttttggat ctttaggaac ttcactggaa atataatcaa taagtgctgg tgcaaagtct
     2641 tcgtcggttt caaaagtttt ccctaatgaa ggatgatcga acaatcggtt caaataagca
     2701 tttccttcca cgacacaagg tgttccggct gccatcgctt ctgtataagt aagcccttgt
     2761 gtttctgaag tagaagcact gacaaaataa tctgccgctt tataataaag tgctacatca
     2821 ttgttgtcca cttctcctgt gaactgcaca aattcttcta actgcagttc tgagatcaat
     2881 tcctctaact cttctacata aggtcctttt cctacgataa ctaatctggc gttaggataa
     2941 gcggagatga cctgaggaaa cccattaatc aaggcttgga tatttttctc ataagaaatc
     3001 ctgcttaaag aaagaatcat caaatggttt tcctgaagac ctagtgactc ccttaatttg
     3061 ctgatatcct gctgagtgat atcagaacgt ttgaattttt caatgtcgat accagtagga
     3121 atcacgcgaa gcggtgctat tacaccatag ctgcgtaatg tatcaataac acgttcactt
     3181 ggacaaacta cacctgtcgt atgatttgca aataatctag acaagtattt tacatgggat
     3241 tgcctgatca cttttccttt accgatataa tgaaggtagt cttcatacat cgtatggtaa
     3301 gtatggatca gaggtatttt caacttctta gctaccatct tcccaagtat ccctgctccg
     3361 aattctgtat gggtatgaat aagatctagt tctagttctt tggcaaccaa ataagcatac
     3421 cacatgcctc taacaacaac tcttcgatcc ttgaaagaaa taaatggtac acttggcatt
     3481 cgaatcacat ctttttccaa cttatccgca ttaggatcgg tcgttgtaaa aatatagact
     3541 tcatgcccat gtctttctaa ttcttgtttt aatgttttga tcgatgtagc aactccgctg
     3601 acttgcggaa agtaggtatc ggtaaaaaag ccgattttca tgtttttcgc ctccaaacta
     3661 gtccttgacc tgatttactt tttttaattt gctagagact ttttcattca gtacactttc
     3721 gtatacattt ttaagttctt caccgatttt attgatactt cgctcttttg ctgtttgata
     3781 accggcttgt gtcgtactcg gtagttcttg attggtaatc tgttggatca gtcggataaa
     3841 ttcttcgttt gtcttcccca tatagcagtt cttgctatct tctaaccagc cattatacac
     3901 tggaatatct cgaacaagta cgttttgttt gcttgctaaa gcttctaaaa caacgatccc
     3961 ttctgtctct tcataagaag ggaaaaagaa tagatctgca cctgaatacg caccttctat
     4021 gatctctcca cgaatgtaac caggaaacag gacattttca ggatgatctt cttttacaat
     4081 tttccggatg ttccgaggaa tcgagtacat gggaacatga ccaaaccaaa taaatgtgta
     4141 ttcgggcatc ttcttagcaa tttctacgaa atcaatgatt cctttacgtt cgaaaaacaa
     4201 accgacacaa atgattactt tttgatccgg agacagtttg aaatattctc tgaacttttg
     4261 ttctttcatt ggatcagggt gatatttctc taagtcgatc ccattggaaa tagcttgaat
     4321 cggcacacgg ataccatagc tttccaacaa ctgttttgaa taaggtgttg gcgtgatcag
     4381 atagtccgct ttttggtaca atcctaccag atattttttt acgataggag aaagctggtt
     4441 cgatctgata aaggaatttc ggaaatcttc ctctgtagaa tgcgcatgat agacaacttt
     4501 ttttccttct cttctggctt tattgaccat attatgacta ttgattccgt atgtattgat
     4561 gtgcaggatg tcataatctt ctttttcatc tagtgtataa gagataccga cttccgtcag
     4621 agcacgtttt tgatgatcaa gcgcacgccc gattccagat tttgcaagca tcttttcact
     4681 ttcaaaatat aataatacct tcaccttacg tcccccttca cttatatagt ataacatagc
     4741 ctacaaactg tcactcaaac ttaagcccta tttttgtcta ttcttactga caaaaacgtt
     4801 ttcttaaaaa agaacaaaaa aactggcgta gctcttttac aagccacacc agcttttgat
     4861 acgttgttta ttttacatat tcatgaacaa gttcaacgac ttcttccatc atatcgcatt
     4921 ctttcaatgc acgatcagca agttcagcca ttttcgctgt gtccaagcgt ttcattaaac
     4981 tacgcgtttt aagaatagat gtcgcactca ttgagaactc atctagaccc atacctacta
     5041 gcaatggaac agctgtttga tctccagcca tttctccaca catacctgcc cattttcctt
     5101 cagcgtgtgc tgcatcaatt acatttttga tcaaacgtaa aattgatggg ttatatggtt
     5161 ggtacaagta agaaacgcgt tcgttcatac ggtctgctgc cattgtgtat tgaatcaaat
     5221 cgttcgtacc tacagagaag aaatctactt cttttgcaaa cttgtctgct aagactgctg
     5281 ctgctggaat ctcgatcatg atgccgactt ggatcgtatc agatacttcc ttgccttcac
     5341 ttatcaattt ttgtttttct tcttcaaaga tcgcttttgc tgcgcggaat tctttgagtg
     5401 tagctaccat agggaacatg atacgtaaat taccatgaac agatgcacga agcaatgcac
     5461 gcatttgtgt acggaacatt tcgtcgcctt gttcagataa actgatacgc aatgcacgat
     5521 atcctaagaa tggattcatt tcatgaggta attgcagata tggtagttct ttatctccac
     5581 cgatgtccat tgtacgaaca actacaggtt ttccttccat accttctaat acagctttgt
     5641 aagcttcata ttggtcatct tctgttggga agtctggaga atccatatac aggaattcag
     5701 tacggtacag accaactgct tctccaccat tattatggac accaactaga tcttttggtg
     5761 ttccgatgtt tgcagctaat tcaaaatgct tgccgtcagc tgtgacagtt tctgcatttt
     5821 ttaatttttc ccattctgct ttttgtgctg cgtaatctgc acctgctttt tcaaattcag
     5881 ctttttgttc atctgtagga tcgatgatta catcgccttc gatcccgttg acagccaaaa
     5941 tatcgccttc tttgacttta gcagtaattt cttttgtccc aacgattgct ggaatttcta
     6001 aagaacgtgc catgatagca gaatgtgaag tacgtccacc aatgtctgtt acaaacgctt
     6061 ttacaaaatt gcgatctaat tgtgctgtat cacttggagt caagtcatgt gcaacaacga
     6121 ccacttcttc gttgatcatt gatggatttg gtaaagtaac gcctaataag tgcgctaaaa
     6181 tacgtttagc aacgtcacga atatctgcag cacgctcttg catataagca ttatcttcca
     6241 ttgcttcgaa cataccgata tacatatccg ttacttcttt tagcgctgat tcagcattta
     6301 cgctgttgtc tttaatattt tgcttgattt gacctaccat ttctggatct gaaagaacca
     6361 tcaaatgggc atcaaatact tgcgcttctg cttcacctaa gctttgcgct gctttttcac
     6421 gaatttgctg caattcttca gtagatttag ctaatgcagc atccaaacga gcttcttcag
     6481 ccgcagtatc ttctactgtt gccttactga atgttaaatc cggttgaact agcaggtaag
     6541 cttttgcaac ggctacaccg tcactagcgg caatcccttt tagcatttca accattattc
     6601 agataagcct tctttcttca ttgtttcaac gattgctgcc attgcatcag cttcgtcagc
     6661 accttctgca gtaatagtaa catcagaacc ttgacctaca cctaaagaca taacgcccat
     6721 gattgatttc aagtttactg atttaccttt atactctaag tttacatcag agttaaattt
     6781 gcttgctgtt tgtactaata gtgtagctgg acgagcgtgg atcccagttt ctgctactac
     6841 gtgaaattct ttcttttcca tattaatcgg tctcctttaa gtgagtgttg tttttatgtg
     6901 agggttttct tttttagtaa ttcagagaat cctaacaaat gccacccttt catctgtaaa
     6961 gattaacatt ttttttcgtt agatacaaca gttttcattc aatttcagat aaaagtattc
     7021 aaattattcc ggattctttt tcatcctttt cttcacattt tataacaaac gtgtttattt
     7081 cctttttctt gtcgctttct cttctcctga cgtttatcat tctttccttt gaccctcttt
     7141 gttttcatat gaaacgaatt acttgcttcc ctttttattt ttagtataat aagagtcaaa
     7201 ggtcaggaat ggtcaataga gatttcacct tttctacctc ataaatctct taaaaaacta
     7261 ggggttattc ttttacctca tgaaaaacga gatgtaaaat atggatgtac ccatttttgt
     7321 gaaaggacgt gaatgattca tgctttgtca aaactgcggc aagaatgagg caacgattca
     7381 cttgtatgcc actgtcaatg gtcagcggac acaactcgat tactgtcaga gctgttatca
     7441 aaaaatcaaa aatcaacaaa atggaggtct gactaatatg gcccaaaatg atccatttgg
     7501 atttggcagt ttggacgatc ttttccgttc catgtctcgc caaatgcaac agcaaggaaa
     7561 ttatgagcaa acaccaccaa cacaatttgg cggaaacaac aacttcaatg gtggtcagcc
     7621 acctcaaggc aatacagaag gtttattggg agaatacgga atcaatatca cagaacaagc
     7681 acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac gaaatcaaac gagtcattga
     7741 aattttaaat cgccgaacca agaacaatcc tgtcttgatt ggagaaccag gtgttggtaa
     7801 aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat ggtgatgtac cgcaaaaatt
     7861 aatggacaaa gaagtcattc gtttagatgt cgtatcttta gtccaaggta ctggtatacg
     7921 aggacaattt gaagaacgta tgcaaaaatt gattgaagag atccgtcaag cagaaaatgt
     7981 gatcctattc atagatgaag ttcacgaaat cgtcggtgct ggttctgctg gtgatggaaa
     8041 tatggatgct ggtaatattt taaaaccagc tttagctcga ggagaattgc aaatggttgg
     8101 tgctactacc ttaaatgagt accgcatcat cgaaaaagac gctgctttag aacgtcgtat
     8161 gcaacctgta cgggtagatg aaccaactgt cgaagaaacg atcagtattt tgaaaggttt
     8221 gcaaaaacgt tatgaagact atcatcatgt aaaatatact gatgaggcca tcgaagcagc
     8281 cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg ccggataaag ccatcgattt
     8341 gctagatgaa acaggttcta agaaaaactt gacgatccaa atcgttgatc ctaaaacaat
     8401 cgagaaaaaa ctgcaagaag cagaagaaca aaaagtttta gcttctcgag aagaagattt
     8461 cgaaaaagca gcttattatc gcgatcagat caataaactt caaaaaatga aagaacgtca
     8521 gctcactgaa gaggaaacac ctgtgattac cgaaaaagat atggaaaaaa tcgttgaaca
     8581 acggacaggt attccagtgg gtgaattaaa agaaaaagaa cagacacaac tgaaaaactt
     8641 agcagatgac ttgaaagctc atgtcatcgg acaagataac gctgtagatc gagtagcgaa
     8701 agcgattcgc cgaaaccgtg ttggattaaa caaacagaat cgtccaatcg gctccttctt
     8761 atttgttgga ccaactggtg taggtaaaac agagcttgcg aaacaacttg cctacgaact
     8821 attcggttca caagattcaa tgattcgttt cgatatgtct gaatatatgg aaaaacatag
     8881 tgtctctaaa ttgattggtt ctcctccagg ttatgtcgga tacgaagaag ctggtcaatt
     8941 aacagaaaaa gtacggagaa atccttacag tcttgtcttg ttagatgaag tagaaaaagc
     9001 tcatccagat gttttgcata tgttcttgca aatcttagat gatggtcgat tgacagatgc
     9061 acaaggaaga acggtcagct ttaaagatac gatcatcatt atgacaagta atgccggaac
     9121 tggtaaagta gaagcaaacg ttggttttgg tgcggcacgc gaaggtgtta ctcgttctgt
     9181 attgaaccaa ttaaacaact actttacacc agaatttctg aaccgttttg acgggatcat
     9241 cgaattcagt gcattatcaa aagaaaactt gatgacaatc gtcacactaa tgctagacga
     9301 tgtcaaccaa atgctggcag cacagcaact tcacatcgaa gttcctacga atgtcaaaga
     9361 aaaattagtt gacttaggct atgatccatc tatgggtgct cgtcctcttc gacgcacgat
     9421 ccaagaacaa atcgaagatg gtatcgctga attttatctt gatcaccctt ctatccatga
     9481 gttgaaagca aaactggata aagatggaaa aatcatcgtt acttctaaac cagaacgttt
     9541 ggctaaagaa tcagccgaag aaacggctga ataaatccag ttttatagcg tgagacggta
     9601 tttctttgag aaatgccgtc tcattctttt ttcagaaaag tcaaaatact gttcatttta
     9661 taaaaaagaa attataataa gagaaacaag caattaaaaa tacataaaag aggtgagcgc
     9721 catgaaattg gtaaatgtaa caaacagcca ttcccgtctt gtattaaacc agcttgaaaa
     9781 cactgatgcc catctagtca aggtctacac agccggaaat accactattg tctacactga
     9841 agcccctgaa cataatgaaa ttctgctgat caatgacaaa cgtaaaatcc agccgaaaga
     9901 aatcgaagat gctaaaaatt atttcttaga taaaatcaaa gacgccgttt accacgagga
     9961 tgaaatcaaa gtaatcgaat tggcaggcct tgttgaaatc tcgattccaa aacatgaatt
    10021 aggcgctaca ttagccgtgt aaacaaattt tgaacataat aattaaagag cgtgggacaa
    10081 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcgaaa gcagaagcaa
    10141 ctccttcgga aataagctga aattcacgaa aattgctttt caaatttttg tgaattccag
    10201 cttatttctt ggagttaaac gcttctgtct cagcctcttt ttctttctag ctttttgatt
    10261 tttctctttt cttttgtact tttctctttt cattttcagt tctacgcgtg tctttatttt
    10321 taactttttc ctctttatgg atgattgatc gtactttttg tgcaatttgc tcgtttttgt
    10381 cccagtcttt tacatctaat aatcgacata atcgtaacat ctcttctttt ttctgttttt
    10441 ttctttcacg tctttgccat tctgtttcca tgtgtcacac tctcctttct ttaatcatat
    10501 gtacgcaaaa taatttttat atttttttga ttagacattt ctatgatttt aatatagtaa
    10561 aaagagccgt cacggaaaat ccgtcacggc tctaaacaaa atatgagagg ctgtttttgt
    10621 ttggctattc ttattacttg atgcagggca gcttgtgtca agacttcttt acatcaaact
    10681 ttttaatttt acattgggat atttatccgc aaaccagcgt tctgcaaatt ggttctcgaa
    10741 taagaacaac ggttggtcga aacggtcacg agccaagata ttgcgacttg aactcatctt
    10801 ctcatccaaa tcttctggat cgatccagcg agcgattttg tgtcccattg gagacatgat
    10861 tacttcggcg ttatactcat ttaacatacg atattggaat acttcgaatt gtagctgacc
    10921 aacagctcca atgatatatt cttccgttag atatgtttta taaagctgaa tcgctccttc
    10981 ttgaactaat tgatagattc ctttgtggaa ggatttttgt ttcatgacat tttttgcagt
    11041 cactttcata aacagctctg gagtaaatga agggagttct tcataagcaa cttttagttt
    11101 tccttcgaac aatgtatccc caatctgata attgcctgta tcatatacac cgatgatatc
    11161 gcctgctact gctttttcga cgttttctct cgaatctgcc ataaattgtg tgacattgct
    11221 taatttcagt tttttatttg tacgttccaa ccagacatcc attcctcgtt cgaatgttcc
    11281 agaacagatt cggacaaaag cgattcggtc acgatgggct ggattcatat ttgcttggat
    11341 cttgaatacg aaacctgaaa attcttcctc ataaggactt accatctgat catcttgtgt
    11401 tttgtgtcca taaggagctg gtgccaattc aacgaaagtc tctaaaaaag tctgtacacc
    11461 gaagttagtc aatgcagaac caaaaaatac tggtgtctga tcgccacgag caattttttc
    11521 ttgattaaat gcatctccag cctctttgat tagttctact tcttctaaga cttgttcata
    11581 taaggaattt tcagttagag gatgattttc gggaagcagt ccatcttcat ccaattctac
    11641 taatcgttca tcctgataat tttccggacg ataaaactct acacgattat ggtagatatc
    11701 ataaagacct tctagccctt tacccatacc gattggccaa ttcatcggat aagattcgat
    11761 atctaatagc tcttctaatt cttctaacaa ttccaatggt tcacgaccat cacgatctag
    11821 tttgttgata aaagtaaata tcgggatgcc acgttttttt acaacttgga ataatttctt
    11881 tgtctgtgct tcgatccctt ttgcactatc gattaccatg accgcactat ccacggccat
    11941 caaagtacga taagtatctt cagaaaaatc ttcatgtcct ggtgtatcca agatatttac
    12001 tcgtttccct tgataatcaa actgcataac agaactagta acagaaatcc cacgttgttt
    12061 ctcgatttcc atccagtctg atttggcaaa attacctgtt tttttgcctt ttacggtccc
    12121 ggcttgacgg atagccccac caaacaatag taattgttct gtaatcgttg ttttcccagc
    12181 atccggatgg gaaatgatcg caaaagttct gcgattgtcc acttgttgct ttaattttgg
    12241 attattcata acctaactcc atttcttcca ttcaattatt tatattttct ctaggcacag
    12301 attgtccgat ctcttaaaga aagacaattt catatagggc tgtgacaaaa gccgtgtagc
    12361 agtctctttt tccgagtaaa cggcgttcta gagacagcct atcagcaata agcccaccca
    12421 agccaaaaat acgaaaagct gtttttgctt ggctgctctt attacttaat gcaggatagc
    12481 tttttcacaa cctttgtcaa ctactacaac tgaatcagta tagcatggat tcagcgaatg
    12541 aaaaagccac caaaccataa gagtcaaata tttttttgtc agctgactaa aagaaaagac
    12601 gtgttgcttg tttattcaag caacacgtct ttcataacca agtttccaat tatcttcttg
    12661 gctcgtcatc ttcgaattct tttctgaagt atcttcgttc ttcttcaggt tccgcttctg
    12721 tttcttccgg atcatgaaaa atgacacgga ttttaaggat tcgagaacct tccatttctt
    12781 cagaaaccag cgtaatatta tcgacatcaa acgataactt ttcgccttca tccggaatca
    12841 tgcctaatgc tgtaatcaaa taaccagcca tcgtatctac atcactcata tgcagatcac
    12901 tgtcaaaagc ttcattgaac tcatcaatca gcatacgtcc ttggatgata tattcgtgtt
    12961 catcgatttt ttcataaaga ttctcaactt catctgattc atcatcgatt tctccgacga
    13021 tttcttctaa aaggtcttct agtgttgcta acccaacgac gccgccatat tcatctaata
    13081 aaatagccat ctgattctgc gtctttttca tttcatataa cagatcatca ataaagaccg
    13141 tttcaggaac gaataaaggc tcctgcatga ttttcttgat atctaaatta tcaaagccga
    13201 atttatgggc agcttttagt aaattctttg tatgaaggat tccgactact ttatctttgt
    13261 cttcgttata aacagggatt cttgaatagt tttcggataa aacttcattc acattttctt
    13321 ccacagtgtc gttgatatca atcataaatg catccgttcg tgggaccatg acttctcggg
    13381 caactttcgt atcaagagaa aaaacccctt ggagcatttc taattcttca ttattcaata
    13441 cgccttcgtt ttcaagcata taacgcattt catctctggt catttttgaa tcttcatcat
    13501 caaaagtcat cggcgtgata cgcgccaata aacttgtgga agcagaaagc agccagacga
    13561 agggcttagc tactacacca atcaagcgta tcagccctga agtgaacttt gcgacttctt
    13621 ctgatttatt taaagcaata cgtttagggt acaattctcc aaaaacaatc gatacatagg
    13681 tcaaaatagc cagcacaatg atatttgcaa tattttttgc cgctgtgccg ccacctaata
    13741 ccggagctaa ccgttccgat aaagtattag ctagtgaagc accagacaaa atgttgacca
    13801 aagtgattcc tacttggatt gtcgatagga aattatttgg attctgcaat atttttaaaa
    13861 gcttttgaga tttcacatct ccttcatcag ctttttgctc gattcgattc ttattgatcg
    13921 aaacaacagc aatttcggaa gctgctaaaa aagcattgat caatgtcaaa acgatcaata
    13981 ataaaatctg cgctaacagc gactgactct cagggtcagc attcataatc gcattctctc
    14041 cttataaaat taatttttag tttacgatag aaaaaaacgt ggcactatag ccacgttaaa
    14101 atggtatcac aaagcaattt tctttgcaat aaaaacaagt gtactaaaac tacctacgag
    14161 gtcgtagcaa ttttcttctt gttttcattt tgcttgtctt ctttgacgat tttgattata
    14221 taggaaatga tcgtcttgca gattgcgtaa aaaggaacac ctaagaagat accaagcaat
    14281 ccggcaatat ttccagctac tagaagaacg ataatgatcg ttaatggatg gattttcagt
    14341 gatttcccaa ttacattagg ataaatgata ttcccatcca actgttggac gattagcaca
    14401 accacacaac aaagtaatgc ttttaccgga tcattgaaga ctgttactag aaatgcaggt
    14461 gctagtccta gataaggtcc taaatatgga attaggtttg tcaatcccgc aatgacccca
    14521 aataagaaag cataatccac accaattacc atatatccta aaaatgtgaa ggtaccaacg
    14581 aataaacatt cgattgcttg tccgctaatg taattagcca atgttttatt cagttgcccc
    14641 aataactcca caatttggtt tcttcttttt tctgggaaaa actgttggat gtttggcact
    14701 agtttttctc cgtctttcaa catatagaac aagatgaatg gtgctgtaat gattactacg
    14761 gttgccgaag cgatcgttcc aacgatcgaa cctaaactat tggatactcc actaaggaat
    14821 tgctgaatga ttgtcccgta agaaatgtct aattgctcaa aatatttatt gatatctact
    14881 tccttgaaca aaggcaattc ggctacctct tttaaccagc tttctacctg tttgatgaaa
    14941 tcaggcatat tagatgccaa tgaggaaatt tgctgtacta gacttgggat gacactaagt
    15001 aaaatccaaa taattgctgc aactaaaagg agcagcacaa ttgcgacagc ccatattctt
    15061 ttgatttttg ctttcatcaa cagcttgaca ataggattca ataaatagta aagaaagcct
    15121 gcaatcagta caggtgcaaa taacgtggaa aaaaatgtcc cgatcggttc gaaaataaag
    15181 tttatttctg atgaaatcat gatcaatgca gctatgatca ataattcaag cgaccaaaac
    15241 attagtttag actgtctaag tttttcaaac aatagatttc ctcctttctt tcttactagt
    15301 atatattata ttcgttaaaa attcttttat tcttttttta tttttgaaaa cttgccatac
    15361 tttttatttg ttttattttc cagtatacta tacgaaagga aggtggctag accatgaaag
    15421 ttgtacaaac aaaagataca atgagcaata tttacttaga tgctgtacgg atcaggcatc
    15481 aagtttttgt agtcgaacag ggtgtgcctt tgtctcgaga aatagacaaa gatgaagccc
    15541 attgtatcca cttcgtcctg tactcagata aaaaagaacc gcaaggaact gtacgtcttt
    15601 tgcctttaga aaacggaaaa atgaaattac agcggatggc gattctttct gaatacagac
    15661 accaagggtt aggaaaaata ttgattgaag aagcagaaaa ctttgctaag aatcaaggct
    15721 ataatacgat tttattaggg gcacagtcca cagctgaaac tttttacgaa aaactaggat
    15781 atacagctta cggcgatcct tttgaagatg ctggcatgcc tcatatttat atgaaaaaga
    15841 ctttataaaa atttatttcg aaaaaagcaa aaaacagcaa gcttttgaga aaaatctctc
    15901 actggcttac tgttcttttt tgtgtttact tacgcgacaa ttgcttgtaa cggttatacc
    15961 aaatatcaat atagccttcc gagaacgggc catctccatt attgatccag tcaaccagaa
    16021 tcttcacatt ttcttttagg atgaagtcaa tatcgttcgg gtaattcatc attttgctgt
    16081 gcatctcata ttcatcgaca tccaatagtc gtttctcgcc gtcaggaaat accttgatat
    16141 ccaagtcata gtcgatatat ttcaaggcct catcgtccag caaaaaaggt gaagctagat
    16201 tacaataata ggaaaccccc ttttctctga tcattgctat tacattgaac cagtattttt
    16261 tatgaaaata aacaatcgct ggttcgcgcg ttacccatcg gcgtccatca gattcagtga
    16321 ctaatgtgtg atcattcacg ccgatcatag agtgttcgct tgtttttaat accatggtat
    16381 ctcgccacgt tcgatgcaat ttgccgtcgt gcttgtaact ttggatcgtc acaaactctc
    16441 cttcttttgg aactcccata gcaacccttc cttaagcagc attttgtgct ttgtgtttgg
    16501 gtgttcatta caccgacatc ataattatta tatcaataaa aatgagcttt gcctagaaag
    16561 gattcctatt gtttcgattt ttctctaact gatctaccag tttcatttgg accttaggaa
    16621 aaacgaccga atcaaatgca tcaggcttca accacttcga agttttgttc tcggtaaatt
    16681 cttgttctgc tccctcggtt gcccgtccgt aaaaaaggag aacatgccat ttcagatgac
    16741 taaaaatatg agtaacttct cctaaatgtc gtgtctgcca aacaaagagt tcctgctttt
    16801 caaatgggag attttgctct tcttctgcta ctaggtcatc aaacagatcg atttctggct
    16861 ttgtttccca ttcttttttc aatcgctcat actcctcttg tgtcacttcc atcattggaa
    16921 atgtccacat attcgccagc aatttttggc tatctctttc ttcaaaataa tacgctccag
    16981 aatgattttg aagagctgca ctgatataat aaacgtcttt tggctttgct ttttttgttt
    17041 ttacaggaaa actcgtttgg attcctcgtt tgttagctaa acaaaaggct tggatcggac
    17101 aagtctcaca ctttggagaa gttggtgtac agatagctga acctagatcc atcattgctt
    17161 ggttgaattc acctggatgc ttttcatcaa tgattttccg catcgcttca tcaaagattt
    17221 tcctgctaga agcttttgcg atgtctgctt caatgcaaaa caatctgctg actactcgca
    17281 tcacgttgcc gtcaactgca ggttctggaa gtccaaatgc gatacttgca atcgcgcctg
    17341 tcgtatatgg tcctattccc ttcaatgagc taatttcttc aggcgtttgc ggcatttccc
    17401 catcaaattc tgacatgatc tgtttagcag cagcttggat gttccgggct cttgagtaat
    17461 aaccaaggcc ctcccatgct tttaaaagct tttcttctgg agcatttgct agctcttcaa
    17521 ttgtaggaaa ccattccatg aaacgataaa aataatcgat cactgtgtct actctggttt
    17581 gctgaagcat gatctcagag atccagatac gataaggatc acgattatag cgccaaggca
    17641 ggtttctttt ttcttgttca taccattgaa tgaactgatc ttgaaattct tttgtttctt
    17701 tatctgtcca tttttcccat tctttattca ctttcctctt ccttttccgt aatgaatcgc
    17761 tggatcatat ccaaatcaaa tcctttttga tataagcttt gctttacttt tagatttctt
    17821 tttttaggtt caaaccttaa atttcttcgc catagtttat ctccttgttt gactaaataa
    17881 tcatattcta tttcttgatc tgcttcttgc ggaagatcag ctatcgcttg ttggatcact
    17941 tcttgagaaa agcctttcgt catcaatgtt tgttggattt ttctcaataa ttctttttgg
    18001 ctttttccgt gattttttcc aaacgctttt tcggctgtct gatgtgcatt ttgaacttgg
    18061 tcttcaaatg tatattggct gagcgcttgt tcaatgattt caggtttcaa acctttttgc
    18121 tgcatctgct gcgctaattt ctttggtcct ttgtcactta agcgcatatt cgtgcgcaca
    18181 taactttccc catagaccaa atcatcgaca aggtccaatt cttttaagcg ggctacgatt
    18241 ttatttcgat cttcttgaag gatttccttt tcttttaagt atgttcggat ttctttttcc
    18301 gatcgcaact gataacttaa gtaattcatt gctaggtgaa aaccgaaatc agctccgcta
    18361 ctcttcttga tctcttggat agtttcttcg tctaattccc gtccttttaa taatcgatag
    18421 cgtactaata catcttcaga tacttgtaaa gcttctcctt cagataaatc tacgcgatag
    18481 aaagggcctt tgcctttact gattctcgta acggttaaca taaacatcct cctcgaacat
    18541 atattctcta tcataccaca gaaaatcccc gtaatcaggt attatatgtt agaataaacc
    18601 acagaaaaaa gaaaaaggga gatacttatg gcagaaacaa ttgtcaaaac tggacaaacg
    18661 gttcctttga aaatcaagcg tttaggcata aatggtgaag gaatcggtta tttcaaacgg
    18721 ttgatcatat ttgttccata tgcactacca aaagaagaag tactagtgaa aatcacgaaa
    18781 gcaactcctc gctatgcaga aggagaatta ataaaagtca aaaaaacaag caaagatcgc
    18841 gtagtggcac catgtcctgt ttactatgaa tgcggtggct gtcaattaca acacttagct
    18901 tatcaagctc aattggattt taaaaaggat ctgcttttgc aagcattgga aaaattcaaa
    18961 ccagcaggct ttcgatctta tcaacttttg ccgacgatcg ggatggatga gccttggcat
    19021 tatagaaaca aagcacaatt tcagttacga aaaaataagc agactcaaaa aattgaagct
    19081 ggtttatacg aagcaaactc tcatcagtta gtccctataa cagactgctt ggttcaagaa
    19141 cctcgaacac aaaaagtaat gaatactgtt gttcaactat tgaataaatt tgatgcaccc
    19201 atctatgatg aacggacaaa cagtggaatc ttccgcacaa tcatggttag aatcggtgtt
    19261 aaaactgggg agttacaagt tgtttttatt actcgcagta aaaaattccc gcaaaaaaac
    19321 gcaatgatcc gtgagatcaa tgaacgactg cccgaagttg tttcaatcat gcaaaatgtc
    19381 caggcacaaa aaacgtctct cgtgatgggg gatgaaacga tccacttatg gggaaaagaa
    19441 gccattgaag aacaaatcaa tgaagtgact ttcgatcttt ctcctcgtgc cttctttcaa
    19501 ctcaatcctc agcagaccga attgctttat ggcgaaggaa tcaaagcatt agatgtccag
    19561 cctaatgaaa cgatcgtgga tgcttattgc ggagttggga cgatcggctt aagtgtagca
    19621 aaacaagcca aagaagtacg gggaatggat accgttcctc aagcaatcgc tgatgcaaaa
    19681 aaaaatgcgg aaagattagg atatcacaac actcgatatg aagtcgggac agcagagcaa
    19741 gttctgccta aatggttgaa tgaaggtttt cagcctgatg gtatcatcgt agatccacca
    19801 cgtacgggtt tggataacgc attgatccaa tcattattga aataccctcc aaaaaaactt
    19861 gtttatatct cctgtaatgt ttctacactt gccagagatc tggttaattt aagtaaaaag
    19921 ttcaagattg aatatttaca gtccgtcgac atgtttcctc agactgcacg ttgtgaagtt
    19981 gtcgtgaaga tgacgagact cgctgactaa atgaaagagg ttgtgaaaaa gtctgccctg
    20041 tatcaagtaa taagaacagc caaacaaaaa tagctcttca tgttttatga atactgtact
    20101 tgtgaccgaa gagttaacag gaggacacta tttatttaaa aaaagggttg tgacatagtt
    20161 tttgtcacag ccctttttga ttattattgg atattcacgt ctatcacttg ataaaatgca
    20221 tttgtcgtat cgctgatatt ccatactgct aaaatcacat tatatccttt tcgatcctgc
    20281 ggaagtgtga tcgtttggtt gactgttttt ggtggaacag ttgctttgtc gtctattttt
    20341 gtgatcaatt caaaatcact aaatttcaaa ggttgattag gattccaagt tggtttggta
    20401 atgtagtaat cccaagtact tgtccgatgt tgtgctgtta aattccaagt aatatttaat
    20461 gttcctggat taaccatgtt tttatgccat ctgttagatg tctgttcatc taaaggctca
    20521 aagccgctga ctcctgcact agcaattttt ccatcaatga atgtattttt ggtagcttcg
    20581 atactttgcg gttcccattg tgctcttccg acattttgat ttaagtttcc tgctgctgat
    20641 gttcctaggt atgcacggct tcctggagaa gtaatataac catgcgcaaa acctggaatg
    20701 ctaaaaccga acaaccctaa actaactaac ccaataaaca ataacgtact aatctttttc
    20761 atgtagaaac ctccttcatt tgataaccac attgtaaacg ctttattgta ttaacacaaa
    20821 cattatgctt ttataatttt atgttgaagt aaaaaaggtg aaaaaagtaa gatgcagatt
    20881 ataacagaag tagacgagaa aagtacaaat agaaaacatg agaatcaaat catatgatct
    20941 ttttcaaaaa catcattcat ataaacaaat gttcaacttt ttaataacaa caattatcaa
    21001 tttttttctt acttcacaaa ctaaatacag gtcttgctgt gagccttcat ggtaaactaa
    21061 tattatagaa aggagttagt aaaatgaaga tgataaaaga tattcgtttt tgggtttgtg
    21121 ttataggaat tgtcatatta ggctttttat cagggttgct tagcggaaat cccggaaaat
    21181 attactattc tttgcaactt ccaccatttg caccacctag ctggatattt gggccgatgt
    21241 ggaccttact gtatatattg atgggtattt ctctttattt gttacttaat cacaacaaca
    21301 aaaaacagcg aaacaatctt gtcggtcttt ttgtgattca gttcattttt aattttatat
    21361 ggtctgctct attttttaat ttaagaaata ttttcattgc agcaatcgat attacacttt
    21421 tagttatttt cttgtcagtg cttatgtatc agttgtggct gcatcatcgc ctggcaatgt
    21481 ggctcatgat tccttattat ttatgggtac tattcgcaac tctattgaat tattcaattt
    21541 attttttaaa ctaagcgcta aaaagagacg tcctgtaatt taacacatag aaaaagtgcc
    21601 acctaagtta gattttttgt ctaatttagg tggcactctt tattaaccaa actatttttt
    21661 tctagtaccc acttgttgtt ccatactcat tattctggtc ataaccataa ccattttgtt
    21721 cctgtccagc gtaaggatca taactttgag tatcttgact tggtgtatag tactgacttg
    21781 tatcttctgt ttgctgataa taggtattcg gatcttggta ctgatcatat gtgttttctg
    21841 aataggaaga ataatcggtg tacccttcac tattcgatcc atcaccgttt gtcgaatcgc
    21901 catcttccga atcatcaaag aacatcattg aagcattctc atttttcaaa ttaggtaatg
    21961 tatcgcttgt ttttatattt aattgtttct tcagttcatt ttggatcgaa agaagatcat
    22021 tttttcctaa aatttgatag tagatatcat tgatcatttg tccttcgcct tgcaactggt
    22081 cttgtttgat tgtttcaaac gccggggtgt agttcgtcgc tacatcaagc atgtcatccc
    22141 aatctaagtc cgtcgtcaca ttgttctcaa ctgcttttaa aatttttcta taattgctta
    22201 cgccatctaa acttaaaact ttattgacga ttttcgtcac tacttcccgc tgacgacgtt
    22261 gtcgaccgat atcgccttct ggatcttcgt gtcgcattcg agaataggct aaaccttttt
    22321 ctccattcaa tgtttgttca cctactggta catggacacc atccaaagta aagtcaattt
    22381 tattattcac tttgatgcca ccgaccgcat tgatcaaatc ttccatgcca tccaaattga
    22441 tcgttacata atgatcaata ggaatatcga gcagttgctc aattgaatcc atggccattt
    22501 cgacaccacc aaatgcgtag gcatgattta atttatctac cgttccataa cctacgatat
    22561 tcgtataaat atctcggtcc aaactgatga tcgttgattt tttttgtttc ggattaactg
    22621 ttactaccat catcgtatca gaacgtcctt gttccgttct tcctaatcca ccggtatcta
    22681 aacctaatag caaaaccgaa aaaggttctt tgtcatcaat gcttactctc tcagaacgtt
    22741 tggaggatat tcgattagag tttttagaaa ttttgtttac tgtttggctc gcttctccca
    22801 taaatctgat tccgtatgct gataatccgg caatcagcaa aacgaatacc cctaacaagg
    22861 ttaatagaac tttttgccat cgcttcataa tacacctctg tttcacattt tctaaaatat
    22921 tgtctcatat aactgttcaa tcagcaaatt c
//
LOCUS       DACCXM010000039        22422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCXM010000039 DACCXM010000000
VERSION     DACCXM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.041"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(139..591)
                     /locus_tag="IVY61_001255"
     CDS             complement(139..591)
                     /locus_tag="IVY61_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7970379.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(604..1014)
                     /locus_tag="IVY61_001256"
     CDS             complement(604..1014)
                     /locus_tag="IVY61_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7970380.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1289..1501
                     /locus_tag="IVY61_001257"
     CDS             1289..1501
                     /locus_tag="IVY61_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7970381.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1504..1716
                     /locus_tag="IVY61_001258"
     CDS             1504..1716
                     /locus_tag="IVY61_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970382.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(2008..2232)
                     /locus_tag="IVY61_001259"
     CDS             complement(2008..2232)
                     /locus_tag="IVY61_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7970383.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2310..2498
                     /locus_tag="IVY61_001260"
     CDS             2310..2498
                     /locus_tag="IVY61_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970384.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2824..3159
                     /locus_tag="IVY61_001261"
     CDS             2824..3159
                     /locus_tag="IVY61_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970385.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3392..4333
                     /locus_tag="IVY61_001262"
     CDS             3392..4333
                     /locus_tag="IVY61_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP7970386.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4335..5225
                     /locus_tag="IVY61_001263"
     CDS             4335..5225
                     /locus_tag="IVY61_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP7970387.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5268..6068
                     /locus_tag="IVY61_001264"
     CDS             5268..6068
                     /locus_tag="IVY61_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7970388.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6083..6934
                     /locus_tag="IVY61_001265"
     CDS             6083..6934
                     /locus_tag="IVY61_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7970389.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7135..7596
                     /locus_tag="IVY61_001266"
     CDS             7135..7596
                     /locus_tag="IVY61_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7970390.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7622..8074
                     /locus_tag="IVY61_001267"
     CDS             7622..8074
                     /locus_tag="IVY61_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970391.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8180..8374
                     /locus_tag="IVY61_001268"
     CDS             8180..8374
                     /locus_tag="IVY61_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970392.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8405..8791
                     /locus_tag="IVY61_001269"
     CDS             8405..8791
                     /locus_tag="IVY61_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970393.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8788..8991
                     /locus_tag="IVY61_001270"
     CDS             8788..8991
                     /locus_tag="IVY61_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP7970394.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            8988..9284
                     /locus_tag="IVY61_001271"
     CDS             8988..9284
                     /locus_tag="IVY61_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970395.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9360..9776
                     /locus_tag="IVY61_001272"
     CDS             9360..9776
                     /locus_tag="IVY61_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP7970396.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10584..10814
                     /locus_tag="IVY61_001273"
     CDS             10584..10814
                     /locus_tag="IVY61_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970397.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            10973..11806
                     /locus_tag="IVY61_001274"
     CDS             10973..11806
                     /locus_tag="IVY61_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP7970398.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11799..13214
                     /locus_tag="IVY61_001275"
     CDS             11799..13214
                     /locus_tag="IVY61_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HAP7970399.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13226..14755
                     /locus_tag="IVY61_001276"
     CDS             13226..14755
                     /locus_tag="IVY61_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7970400.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14847..15725
                     /locus_tag="IVY61_001277"
     CDS             14847..15725
                     /locus_tag="IVY61_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP7970401.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15727..16044
                     /locus_tag="IVY61_001278"
     CDS             15727..16044
                     /locus_tag="IVY61_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970402.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16093..16794
                     /locus_tag="IVY61_001279"
     CDS             16093..16794
                     /locus_tag="IVY61_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP7970403.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            16809..17699
                     /locus_tag="IVY61_001280"
     CDS             16809..17699
                     /locus_tag="IVY61_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP7970404.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17722..17937
                     /locus_tag="IVY61_001281"
     CDS             17722..17937
                     /locus_tag="IVY61_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970405.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17949..18290
                     /locus_tag="IVY61_001282"
     CDS             17949..18290
                     /locus_tag="IVY61_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970406.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18290..18661
                     /locus_tag="IVY61_001283"
     CDS             18290..18661
                     /locus_tag="IVY61_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970407.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18654..19049
                     /locus_tag="IVY61_001284"
     CDS             18654..19049
                     /locus_tag="IVY61_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP7970408.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19051..19428
                     /locus_tag="IVY61_001285"
     CDS             19051..19428
                     /locus_tag="IVY61_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970409.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19429..20037
                     /locus_tag="IVY61_001286"
     CDS             19429..20037
                     /locus_tag="IVY61_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7970410.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20037..20378
                     /locus_tag="IVY61_001287"
     CDS             20037..20378
                     /locus_tag="IVY61_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970411.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20620..>22422
                     /locus_tag="IVY61_001288"
     CDS             20620..>22422
                     /locus_tag="IVY61_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP7970412.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAIN"
BASE COUNT         8119 a         3274 c         4637 g         6392 t
ORIGIN      
        1 tttagtgatt cctataatta ataaaataag tccaatcaat aataacattc aaaactcctc
       61 atttctttga tataattatt gtagagttta ctcagaaatg aggaaagagt ccatgtgaaa
      121 gcatgggctt ttttattttt aatgttgtaa tactaaatgt tcaagattat gaggaagccc
      181 taaatattca ataatttgga actgactcat atcagataaa tcttgttcat ctattgttct
      241 agttaacatt tcaattgcaa acatattagc ttcagcttct ataccataaa tcatacaact
      301 aaggttgttt gctctaaaag ctgctgtgca aataccttta tggagtctaa cgtgggctat
      361 ttcatgccat aatacaaatc ttttcataga aggtgatagg ttttgattaa ttaatatcac
      421 ataacatctt ttattgtgta tcgtttgtcc taatgtttta ttccctaaat cagtttcaat
      481 taaatctata tcagaatatt tgattatttt atatggatta atcgcatcag atccaaactc
      541 tacaaataat ttttctattt cttctaatat ccagtatttg tttaaatgca taccaaagcc
      601 cacctattga cgatattttt taggagtgaa ttttttcttt gctttaactt tggttaaggc
      661 aagtgcaagt tgaatggcat cgtataattt ttccttatct tcatcgtcta aaggttcacc
      721 ataataattt gtttcagcat ctgaattcaa gccttcaagt aatttttcag cttgtaaagc
      781 aatatcttgt ttatcttttt ctgttaaatc ataataatgg cgtttatcag tacgtcctaa
      841 aagataatca gttgatacat taaagtaatc tgcaactttt tgtattttat ctccagatgg
      901 agtactttta ttccatctag acatgcttcc atttccgaaa tttaactttc tttcaagttc
      961 tgccaacgtc attgaacgtt cttttaataa gatttttata cggtctaata gcataggata
     1021 tgcattcctt tcaaaatacc gacaaaaaaa gtatttttct gcaaattatc tatttacaaa
     1081 tagagaattt gcagttatac tagttttgta agcaagatat ttacggcata caaagactaa
     1141 aagcaataaa ctaatagtct ccccagacat cagtaagctc gctttttgtg cgctgtattt
     1201 tgtatgctta cataatagca tatttgcagt ttttgtcaaa cttattagac aaaaatatta
     1261 gaaaatattc gataaggggg tatgaagcat gactttaaaa cagcaagtat taattgcttt
     1321 agtaaagaaa ggttggagtc aaagagagtt agctcgacga atgggtatat caattactta
     1381 tttacgagat atcttcatcg aaaaaagaaa acctaaggaa cgtttaaaac aaatcgaaga
     1441 acttttggat atcaagttag aagtagattc taatgatcag ccatcagaac aggaggcgta
     1501 aaaatggtac aacgtttgag caaagcggag tttaaagaga aatataagta ttctgaaagt
     1561 acttatcaaa gaagaatgaa ggaatttaaa aaatcacgtt ttagtgaggg gtataacgca
     1621 gttacttcca aagaaataac aattgatgtc gatttatatg aacagtttag aacatggcgt
     1681 tctaaaaatc gatttagagt tagagctgtt tgttaatagc taaaaaatct aaattcacca
     1741 cttctgaaaa gaggagttgc tgctctcctt tcaagtttgc caaagtccac gttttttcat
     1801 tttggcaact cctcttcttg gaggtggtga cggcgagaga cgaaggaggg atgacatgaa
     1861 ggaagaatca aaaataggga ttttgaatga gaacaattac acaagattaa ttattgaaac
     1921 agaagaaggg aaaaaagtag cagaagttac attgaccgat gcaactcctg ctgacggtta
     1981 tcgtattcga ttaactccta aatatgatta accttttggt gggtgtggat cgtttccgtg
     2041 actatcacgg ctttgaattt ttccatctct gccatgaata aatagctcag atccttgatt
     2101 tttagaaatc tctctagcga tttttactgc ttctgatttt gtattagtat gtgcagtcgc
     2161 tttagaattt ccagcacctt tgacattcca accaccattg gaattaggta caacatgttg
     2221 attttttccc atagttttca cctccatttc tatagaaata ttttatcaaa tagaaatgga
     2281 gaaattcaat aaacgaaagg agaagtgata tggcatatac cttgcaacaa gaacatcaaa
     2341 ttctcggttt gattaaacaa cgcaggaaac aattacaaga tgaccgtgca gcgcttagaa
     2401 aagccgatga gctatcagat aggcaagctg aactaattgc ttctgaactt gaggatttga
     2461 gaatgctaga aataaaaaat agggagatta gattatgaag aagacagaca cactttttat
     2521 aggattcatt ttggggttat tagtgattgt agcgcaccaa agtattatcg ggggaagctt
     2581 gttcgcagca ttgatggttt taatcaatct gcttgattca aaagaaagga gcaactatgg
     2641 cacgagaaga agcgctaaaa atcggtaaag tgattgctga taatcggtgg ggaaatagcc
     2701 gccctattat tttaagcaag caacatatcg aaaagcaaaa agcatggcaa caaataaaaa
     2761 agtgactctg ccagccagca aagagtcaca tacaaattta taaaactagg agaattatat
     2821 cacatgaata agaaaattga aaatcttatc gaagaattga aacgtgaatg ccaaaagcaa
     2881 ggtgtttcta ttatttgtac agcccaaaaa gaaggggaac ttaaaagtct cgtttatggt
     2941 gagacaacag aaattttact ttgtcttgct atgcaagaag aacatttaga tgaaaatctc
     3001 ccattgtcag cacatattat gcgtagaatt gctgtggatg cttacgaaca agcgaaaaat
     3061 gaagaagaaa atcaaccttc aaatcatact tttgttatta ataacaagga agatttagct
     3121 gatgtgatga ctcgtattct caagggggaa tttcaatgat gaatgaaaaa acaattgatc
     3181 tactcattat tttatttttc acgttagtga ttgtagcgaa aagttatgta atctttggct
     3241 tgttattcgc aattaccatg cttgtgcttt gcgtgttagc aagcagaaaa gaggttaaac
     3301 atgaacgagt tagcaaatct tgacaactat ttaactgatc ctgattatgc taaaccacca
     3361 tatgaagcac caattgatga ggaggatgaa gatgagtaaa tctaccttag aaatgagcca
     3421 tcaagaatgg cttgaagacc gtaagaaagg catcggaggt tcggatgttg gaacaatttt
     3481 aggattgaat aaatggaaat ctccttatca actatggcta gaaaaaacag gacaagtagt
     3541 acttgaagaa tcaggaagtg agcccgctta ttggggtaat gttttagaag aagtggttgc
     3601 taaagagttt caggaacgta caggcaaaaa agtacgcaga agaaaccaag tatttgaaca
     3661 tccattacat ccatttttaa gagcaaatat tgatcgggaa gtagtgggag aaaatgccat
     3721 tctggaatgc aaaacagcta atcaatttct cggtaaagaa tgggaaggcg aagaggtacc
     3781 gcttagctat ctctgtcaag ttcaacatta catgaacgtt ctaaacaaag actattgtta
     3841 catcgctgtc ttaatcggtg gtcaaaaatt tatctggaag cggattgaac gagatcaaga
     3901 gctgatcgat acaatcactg aacaattagt agagttttgg gaaacaaacg ttcttggagg
     3961 tatcgagcca attattgatg gtagtcaagc gacagctgat ttcttgaaag agaagtatgc
     4021 agatgttgaa gacgttcaaa cgacgttacc aattcatttc gatgaactag ttgaacagaa
     4081 aaacgaactc aaacggacta agaaagaaat tgaatcagct atccgtcaag tagacaacga
     4141 gattatcagc gagctaggta aacgtgaagc cagtatcggt atcactcaaa aaaacatcat
     4201 cagttggaaa tttgtccgta cgagacgtat gaactcgaag aaactagcag agaaatatcc
     4261 agatgtcgca aatgatgaag agatttataa cgttactgaa tcaagaaggc taaccgaaag
     4321 ggagatcaaa taatatggca acaaatgaat cgttaaaaaa tcaattggca gcaaagccac
     4381 agaaacaagt tgcaccaggt caattaggac ttaaagcttt gatgaataca ccaacaatga
     4441 gaaagaagtt cgaagaggtg cttcatgaca atgctaatgc ttttatgtcg aatgttatga
     4501 ctcttgtatc taatgacagt tatcttgcag atagtgaacc gatgtctatc atgagtggtg
     4561 cgttaactgc tgcaacatta aatcttgggc tagataagaa tttaggttat gcatatttag
     4621 ttccattcaa tagtaaaaac aagcaaacag gaaaatggga aaagaaagct caattcatgc
     4681 ttggctataa aggatatatc caattagccc aacgatcagg taaatacaaa gcattaaatg
     4741 tgatcgaagt ttacgaagga gaactaaaaa gctggaaccg actgacagaa gagtttgagt
     4801 ttgatccaaa tggtagaacg tctgatgaag tcattggata tgttggctat ttcgagttac
     4861 tgaatggatt caaaaaaact gtctattgga ccaaacaaga aatcgaagct catcggattg
     4921 ctaacaataa agatcgcgat aagacaaagt taagtggtgt gtgggcatct gattacaatg
     4981 caatggcacg aaaaactgtt ttgagaaatc ttctttctaa atggggaatc ttgtccatcg
     5041 aaatgcaaga agccaccaca tcggatgaga gagtccaaag ggttcaagaa gacggcagca
     5101 ttattgctga aacagaagtt gaagaagata ttcctgaaag aaaagaagca gaggttatat
     5161 ctgaagaaaa tgaagatgta caaactggat tatttgatgc atctaatccg ccgttaaaca
     5221 aataatgagg gagctttctc ccttgctttt ctagaggaga aatacgaatg aacacaggat
     5281 atgtcaaatt acatcgaaaa gtgatgaatt cattcgtttg gaccaatcct tatatgtata
     5341 aattatggaa cttgtgtttg atgaaagcga gtcatgagaa tcgcaagata ctttttaatg
     5401 gaaaagagat acaagtgaac agcggagaat tcgtcacagg gcgcgatgct atcacatctg
     5461 agatgaacga aggcgtgaaa cgtgaacatc aagtgaacag cggttctgta tggagatggc
     5521 taaaacaatt tgaaaaaaac ggaatgttga acatcaaatc aactacgaaa tacagcgtta
     5581 tatcaataaa aaactggtct ttgtaccaaa gcagtgaaca acaaatgaac atcaaacgca
     5641 caacaagtga acaacaagtg cacacaaaca agaatgaaaa gaattataag aatgaaaaga
     5701 ataataaaac atcatcgtcg caaccacgca aaaagcgtgt ttacgacacc gactcagttt
     5761 actacattct cgcggaggag ttattcaaac agatttgtca gaatcaggaa atcaaaaagc
     5821 cgaatctgca aagttgggca gataacattc ggaaaatgat cgagatcgac aagcggaccg
     5881 aaaaccaggt acggggaatg attgaatgga gtcagcacaa tgtgttttgg gcatcgaata
     5941 ttttatctgc taaaaaattg cgagaaaaat acgacacaat ggcagcgcaa gccaatcgtg
     6001 attataaaac aaaacaaact aaaacgcttg aatacgagaa atttagcaca gatgagttgc
     6061 ctatttgaga ggaggcgtag gcatggaaac tgttggggaa atcatggaaa agctgataca
     6121 gaaagtgctt gtccaacgtg gcgaatgtcc tgaatgcgga cagcctttgt atggatggcg
     6181 tacgaagaac cctgatggct cagaacgttg taagccaacc tgtatgcaat gtgggtataa
     6241 agcattgcga gttcaggaag acttacaaac agaacgaatt tacaacgaga gcctgaaagc
     6301 aagagcaatc aattttttca aaggtggttc tgttgtgcct aatcaagcgt tgtttgattg
     6361 cacattgcag aattatcaaa ttgtcgatca agaaacaaga caagcggttg aagtaaccaa
     6421 acgctttgtt aattcagtct tgttaggaaa tccaagtcac cttgttttaa ctggaaaaca
     6481 aggaacaggt aaaagtcacc tagcgatggc agcggcttgg gaagtcttga agcgatcaaa
     6541 ctacgataaa aaaatcttat ttatcgggtt acaggaaatg ctggatcaaa tcaaattttc
     6601 gtacaacaat cctgaactca gaaaaacgat tgagggatcg ttgattgcag atatcaaaac
     6661 agcggattta gtcatcattg atgatatcgg ttcagaacta ggaaaagatg catcagatag
     6721 tcgagcgttt ggcataaaca cgctaaattc gttcttggat gcacgacaga acctagcaac
     6781 gattatcaca acgaacttgc ttggtgaaga actgaaaaaa gcttacggta cgagaacaat
     6841 atcaagaatg tttgtcaact ctgatggatt tacgatggta ttttctcaaa cagcagacaa
     6901 gcgcataaaa ccagtcaaag gagcgatcgc atgaatttga ctaaacaaga aaaaagaatc
     6961 tgtcgtcaga taatgttgca aattataggt gatcgtgaca tggacgaatt atctcaacaa
     7021 gagagtcgaa tggttcggaa gataagtaag ctaattattt atcgggatac agaaagattt
     7081 ctcactgttt taggtaacgt ctacgagaat ccagagttat tggaggtaga gtaattgcca
     7141 aaaatattag atgcatgttg cggtagtaga ttgttctggt ttgataagaa caattctgac
     7201 gtaacattta tggattgtcg tcagcagtat gaagaattat caacaggtca tgtgatcaat
     7261 gttgatccag atgtggtcgc agactttcgc gatatgcctt ttgaagatga tgtgttcgat
     7321 atggttgtat ttgacccgcc acacttaaga catgtcggcg aaaactcatg gcttgccaaa
     7381 aaatatggaa aattagacga actttggcca gaagacatca ggcaaggttt tgcagagtgt
     7441 atgcgagttt tacgaccttc aggaacatta atttttaagt ggaacgagga acagatacca
     7501 ctttctgatg tgttagaggc tattggagaa cagccattgt ttggtaataa acgaagcaaa
     7561 acacattggc ttgtgtttat gaaaaaattc agctaacgga ggaaacaaag gaggaaaagt
     7621 aatgaagatt gtattaaata aatgttttgg tggctttggg ttgagccatg aagcaaaaat
     7681 ggaaattttt aaaagaaaaa atattgaagt attcccatac atcaataact ttagctatga
     7741 ctcagatgat gaatacacga gatacacagg ccaaaagcta ggttcgatgg attttatcta
     7801 ctatttcaaa aaagacccga aaatcgacaa agttacagga atttatagcg aaattgaccg
     7861 actgtacgga attgctgatg attccagttt ttcaagtgat tcaaacagag gtgataagga
     7921 cctcgttgct gttgtcgaaa aactaggaag tgaggccagt ggaccatatg caagtcttaa
     7981 agtcgttgat atacccgatg gggctgagtg ggaaataagt gattatgacg gcgttgaaac
     8041 tgcacattat ggttttcaaa caggtagtat ttagtccact aatgacaact aaagaggagg
     8101 cagaaggatg agtgaagatg aatactttga acagtagtct attaacgaca gagattttta
     8161 actggaaagg ggtgtagtca tggacgcatc ggaagtagta aataaaatta atagtctcaa
     8221 agaggttttc ggagacgtcg aagtagtgat aaacgcacaa ggctatgaca acggattttt
     8281 taagcagatt accgacatta gcattcagca aggaatggaa gacgaagatg gaaactttat
     8341 cgatgaagta gccattttag tgacttgcga gtaagtaatt gacagctaaa gaggaggcag
     8401 aaggatgaaa aaattgcacc aactcatatc tgagaaagaa agcgaactcc aaaatttaga
     8461 agatagtttg ggattggggt ttccaattgt tgagcaagct aaaatgactc aaatcagtca
     8521 tctacgactt gaattggaag atttaagaca aatagaaaaa tcaattcaac tcaacgacaa
     8581 ccagaaaatc gtgttggagt ggttgaaaag cgagacaatt ttaactagag aagcaccaat
     8641 attatctgtt aatgcttttt ctgataaaaa tttactagga aaattacctg ataaagtacg
     8701 caaagcttat aaactattgg cttgtaaaca agaatatgaa gtcctatcag cattcgctca
     8761 atggggatta gaacaggagg aagcggaatg aacaacagac atcgcagaat aacaaaacta
     8821 agaaaacagg aactgaatgt actaaagaca aagtttgaaa aagaatatgg aatttcagca
     8881 gaagaaacat ataaagtggc aagtcagttt gttgctgatg cgagtaatgc tattcgtaag
     8941 tttgggattt cgatattaaa tgatgatcgt aaatgggagg aaatgagatg aaaataaaag
     9001 acggatttta cgctagtagt catggtatcg gcggtttaat gctagacatg ccgataaaga
     9061 accctaaaac acgtcagaaa tcaaaattca aaatcggtga catggtccga tgtaaagcag
     9121 aagagttcat ctatccgttt cgtggatatg tagagcatgt ctataatcat tcagcaatca
     9181 ttcacattga aaacacgatg gaatgcgata agtggttagc gaaaagcaaa gataatttag
     9241 cagtggctcg tttggtggat atggaactaa tcaatgacaa ataaaaaagc cggatcgctc
     9301 cgactgattc aataaatcca acacatttat tatatcacat aaaggagcgg tttgacttga
     9361 tgcaattgtt acgagaggta gatttcaaac agacaagatg taatgcgaga gatgtgctga
     9421 agaactttcg gcgtttggag cggatggcag gtcgctcttt gatagatatt aagtcgccga
     9481 ttattacgga tatgccgaag gcaccgaagc acggcaataa ggcagaagac gcgatcattc
     9541 agatgatgga tatagaagcg gagagagacg caatactagc ggctttgatg gctcttagtc
     9601 tgattagccg tcagatactc tactacagct tctgtgacgt aaacaagcac tctaattatg
     9661 aaatagggca attgatacga ggatacggag agaagaatgt agagaagttg aaatccatcg
     9721 cattaatcga atttgcagaa gcatacaaaa aaggcgtgtt agttcagtat cgttgatttt
     9781 gtagggtttt tgtagggata gtgtagggtt tttgagcggt ttaacgtgat attatggtag
     9841 tgtcgaaaga ttagtgatag gtctgagaca aaataaaatg taagggagga aatctccctc
     9901 atcgttttaa cttaagcttc gatagacagc aacggaaata ttaagaataa ggatgtgaat
     9961 ttcaactcct tctaaattgt tcttattatc tatcatccgt tgttgtctat taatttatgc
    10021 atcggaggaa aaacgaatgg ataaagaaat caaagtaact gtcaaattag atttgactga
    10081 actaaaagaa ctgctcaaca aggctagtga ccaagtcgaa cagttacaag aaactttaga
    10141 tgaaattgct aattttaaaa tccaagtttc ttagcagtat attttttagc agctgcactc
    10201 ataaaatcat accaagtatc agaaaaatat aacttaacta aataattaaa aaaggtgggt
    10261 aaatagaaat gacggcgtta ataatttcaa tttttgcgct ctgtcttaat gtctatatga
    10321 ttggatttaa aaatggacaa aataaaaaat agtagcagcc gagaataatt ttatagtgtc
    10381 actgtggcgg aaatagaaga cgcaaaggtg gaggtaggaa gcgtatggcg cactatcggg
    10441 accttgtaca aaactagaaa ggactagtac gtgtgtggtg cgagccctag ggaggaacag
    10501 gataaccgcc tgtgtgtagg ttgctattac atattagatc actctttgag tggtcttttt
    10561 atttacataa aggaggctac ataatgagaa actactggta tatatcgcta actaatgaat
    10621 atcctcgaac cattgatgat tgttcagtgc gtgttgtgcg ttctgtccaa atcaaaaaga
    10681 agtactccat tgttgaaatg accagagaag ctacgccaaa agagattgat aaatgcaaac
    10741 ttctttattg cggtcatggt ttctatgatg aaccaaacat tcaaaataat attaacaaga
    10801 atttgaggga ttagaatgca agaaatggct taaaaatctc caatcaggac aaatcaagtt
    10861 agacaaagtt tccgatttaa agatattgat tgaagctgat ttaatgctaa aagatattga
    10921 aaattagaaa acaaaactca acctaagaag attgcgaggt ggtgtgtatt gaatggcaag
    10981 acaacgtgat ccaagacgtg atgaagccaa aagaatttgg ttagaatcca acggagaaaa
    11041 gcagttaaag gaaattgcat ctgaattaaa tgtttcagat tctcaggtta gaaaatggaa
    11101 atcgcaagac aaatggagcg ctgaattgaa aagtaacgtt accaatggca aaagtaacgt
    11161 tactaatcaa ggtggcgctc ctattggtaa tcaaaatgct aaaggtaaca aaggaaacag
    11221 cagagcatca ccgccaaaga gaaataagaa tgctcttaaa actggtgagt atgaaacaat
    11281 attttttgac acgttaaccg ataatgagaa ggacatctat tctagtttga atgatgatcc
    11341 ttcttttgct ttgtccgaag aaatacgact tcttaaaata agacaacttc gaatgatgaa
    11401 aagaatccaa caagctgaag ctggactaaa tgatgaagaa gtcgaacgat tgcagcagct
    11461 aagaaagatt aaaactccga ttgaaaaaaa tggtaaaaag ctagaaatca agcgtgaggt
    11521 tatgcaagat gtgcagatta gcagaaaaaa acatcgcaaa attgatgata ttctttcaat
    11581 tgaagattca ttgactcgga ttagcaacca gttagctaaa gccatcaagc aaatgaatga
    11641 actttatatg aatgaataca gaactgattt aattaaagct cagactgata agatccaagc
    11701 tgagacaaat gaaattggcg gaaataattc aggtgaagaa atagaagaat ggaaacaggc
    11761 agttttaaat gccgcaaaca aacgggcggt gaaagaaaat gaataatgaa tttattcctt
    11821 ttgctgatat tggttctgcc attgattatt actatgataa accagtagct ttttgccaag
    11881 atattttgca tttgaatcct gatgaatggc aagaaaatgt tttaaatgat ttagctgaat
    11941 tttcaaaggt ttctgttcgt tctggtcaag gagttggaaa aacagcatta gaagcaggag
    12001 caatactttg gttcttaacg tgtcgaccct acgctaaagt aatagcaaca gctccgacaa
    12061 tgaagcaact ttacgatgta ctttgggcag aggtagctaa atggttaaat gatagcttga
    12121 tcaaaaactt actgaagtgg acaaagacca aaatttatat ggttggtgat tcagagcgtt
    12181 ggtttgctac ggctagaaca gcgactaaac cagaaaatat gcaaggtttt cacgaggacc
    12241 atatgttgat tgtggtagat gaagcttctg gtgtgtctga tccaattatg gaagctattc
    12301 ttggtacgct atcaggtttt gataataagc tgttgatgtg tggaaaccct aataatattg
    12361 aaggtgtctt ttacgattcc cacaattcag accgtgataa atacagagtt cataaagtat
    12421 caagctatga tagtaaacgt acaaacaaag acaatataga aatgattctt aaaaaatatg
    12481 gaaaagaaag tgatgttgct cgtgtccgta tttttggaga atttcccaaa ggtgcgttgg
    12541 attcatttat cagtcttgaa acggttgaat tggctacaga aaaacaaatt agtgattctt
    12601 tagtcaataa aacaacggtt gctcatattg gtgttgacgt agctcgatat ggtgatgatt
    12661 ctacgattct ctttcctaga attgctacca gggcattgga gtatgagaag tattcaaaac
    12721 gtagcactat ggaaacaaca ggatatgtca tcaacatggc caagaatcta atgagtcaat
    12781 atccgagtat tgataaagtg atgattaaag tcgatgacac tggtgtcgga ggtggtgtaa
    12841 ccgaccgcct agaagaactt atagaagaca agcattatcc ttttgaggtg tttggagtga
    12901 ataacggttc aacatcagaa gacgattttt acgataattt aggtactcaa ctatggggaa
    12961 acatcaagga aatgttagaa gaaaatatga cagcaaatct taacggagaa cagcctgtta
    13021 ttgaattgcc ttctgatagt tcgttaatca aagaattaag tactcgcaaa ttcaaaatga
    13081 caagtagaag tcgtatacgt ttagaaagta aagatgatat gaaaaagcga aatattggta
    13141 gtcccgatat tgctgacgca ctggctttag cgttttatga gccaccaagt cactatcaat
    13201 ttattcaatt ttaggaggtg agtttttgat tagtacagta ttatctttag agagatataa
    13261 gaagctacgt gttaaatatg caacacaaat tgaagatgga atgtttgatc cgaatggttt
    13321 tatagaatat atgaaaccat tttttgctga tcgcgaaaga aaatatctag cttatactag
    13381 cgaaaagaat gaaatagaca atagaccaaa gcctaacact gatattgtaa aagttaataa
    13441 taaacttcat gctggtatgt attcaattgt cgtagaccaa gcagtcaatc attttactgg
    13501 tatacctatc aaatgggatt acgatgtatc ggaacaaaaa agaacactca ttcaaagatt
    13561 aaaagataaa ttcttaaaaa atgatataga acttccaaca gttccagaag cttttaacaa
    13621 attaacaagc aatcttgatt ccatgagatt cgcaatgctt gattctgaaa ctgcaacttt
    13681 tcaaggagct tgtggagtag ctttccgatt gttagagcct gtggaagaag atgatggttg
    13741 gaagttaaga gcaagtaata ttgaaccttg gagagcagaa agatatggaa atgccggaat
    13801 ctatatcaaa gaaaagtatg actcttacca gaaaaaattt tttcaagaaa tgaaagttat
    13861 gacaagaaat aaaattctga catatgcttg ctatggtgat ttgaattttg ttacaagcgg
    13921 aacgttcaaa aaaattgatg agaccgataa ccctttgggg acgattacct tggcagaatt
    13981 taaaaataat acgaatcgtt attgtgattt tgaagtagct gaggaaattg gagatgcaat
    14041 tgatcgtgct ttgtctgatc aacaaaacga aatcgaacaa tttaagcttg cgtacatgct
    14101 tgttactggc acaacaatga gcaaaggcac tgctaaagaa atgatgaatc agctagggat
    14161 tatcaactta aaagacccta ctgcaaaagc tgaatatgtc acaaaaaaat tagcaaaaga
    14221 tttcaatgaa tatcatatgg atctattgaa aaaacagttt tacaccattt gtaaagcaat
    14281 cgattttaac gatgaggtgt ttaaatcaaa cagttctgga gaagctcgca agtggcaaat
    14341 cattagccta gaagcgaaaa caaatactaa agagcaatat tttagagaag gattgaaaga
    14401 atgcgcagaa acaattgctg cttttcttaa gtttcatgac aaagtagaaa ttgagcctga
    14461 aaaaattatt tttactttct ctagatcttt gccaactgat ttaagttatt tagctgaagc
    14521 tttacctaaa cttgcaccat acgtatccaa acgaactatt cagagccaaa ttccatttgt
    14581 aacagatgtt gattatgaaa atgaaatgat ggaattggaa agtggaagtg cttatccaga
    14641 tagtgaatac aattttggcg gaggtggcaa tagtgacgat agaaacgaag tattggacca
    14701 aacgtcgaga actggaggat caagcaaggc tcaaacaaga aaatcagaca cttaaaaaat
    14761 taactagtgt atttcctgaa gcactgaaag agatacaagc aaaactatta tcacaagctg
    14821 acttacacaa tatcacttat ccagaaatga tggagtttta cagtacaagt aatcagaaaa
    14881 aatatcgtga atatgtggaa aaaaattata agtcattaaa aatgtatgat gcaaaataca
    14941 aagagtttat cgatgaattt tttccaccat ttgactatgc aaaagtcaat cgcttattac
    15001 aaatacgatc agatgtattt aaaattcttg cagaatatgc gatggatgca gatgtgaatc
    15061 aatatttttc tgatcgctta gaggaaattc ttcaaagaac atattcttct aatgctaatg
    15121 tttttgttca acttttaaac gttgatatac caaattattt accagaaaat gaaattaaac
    15181 attatttgaa ctatccatgg tcaggaaaga cattttcaag aagactttgg ggaaatattt
    15241 catcgcttga acagaaactc tctaatgcta ttgtaaaaag tgttgctagt ggagaaggcg
    15301 ttatacacgc attaaacacc atgagattag actcagaaat ttgtgacatg tttaagttag
    15361 aagaatcaaa gtataacaaa gcgatagaaa atctcgttcg aacggagtat gcaaaatttg
    15421 cacaagatgg tattgaaaaa tcatatttag aaacaggtat tgaggaatac aacgtattga
    15481 ctgcaaaaga tgagagagtt tgccggattt gtggaggaaa ggcaagtaag aatccctata
    15541 aactgaaaga tgctgtcata ggtgaaaatc gagcaccttt ccatagtcgt tgtagatgta
    15601 cggatgttcc taatttacca aaattaggaa aggatattga tgaagaatat gaccgtttat
    15661 ttggcgattt attagatgag tttgcacatg attcttttgg aattaatttg aaacggagga
    15721 agtagaatga aagatttttt tgaagcagta ctaacaatta atgtaaatgc tgatattgca
    15781 gaagcctaca aaacagctat tgagtctgag aaccatccta atggcttgag agaccattgg
    15841 aatggcaatt atgcctacgt ggttattggc gatcaaactg ttaattatca agataatact
    15901 ccagttgata agaataccgt taatttaacg attcaattat tatctcattc attaccaaat
    15961 ttaaaagaaa cagttgattg gtatgaaaag atgggatgta ttgttgttag aactgactac
    16021 aaagaaggaa agtctagtaa ttaggctttt ttattttgtc cgaaatgacg ttaaactagc
    16081 gcaatactgg gcttggttga atggtggggc gcaactatta aaactcaaag caatgcgggg
    16141 cgtgaaaacg aatcgtgggg cgaaaggaga atgagcatga aacacaaatc attaatgcca
    16201 atgaatttgc aatactttgc cgaaggtgac gatcaaaagt tttcttttga tgacttcaaa
    16261 tcttttgtgg aatccaatga agaagctcaa aaatttattc agtcacaatc tcaaagtgtt
    16321 gccgacaaac aattggaagc ttggaaacaa aataacttag ataaaattaa acaggatacc
    16381 atcaaggaat atgaggaatc taagaaaaac aagtcacctg agcaaattca actggaaaaa
    16441 ctacaagctg aatttgaagc agaaaaagcg ttgcgtgtga caagtgataa taaagcattt
    16501 gttgcagaac aaattgctgg attagaacta gatggagagt taaaagagtc tatttctcaa
    16561 tttatgctaa ataatcttgt tagttcggat acagatttca ctaagaatgc tgttgaaggt
    16621 tttacaagtg ttttgaatgc aattaatgag aaacatgcag atgcattaaa agaactacaa
    16681 atgaagtctg catttggtgg aactcaacaa tcgaataacc aggttcagca gaacaatgaa
    16741 acattaacaa atccagaaga acaattagga caaattcttc aacaatttaa ctaggagagt
    16801 gaaaaattat gaaaaaaaca tctttaaata atttagagta tttggatatt tcaccagcga
    16861 ttaatgctat gcaagtacca aatacacctt ttttaagcta tttacttggt gctggaaaaa
    16921 cagagccagc aaactcgaca gaaattaaat ggcgtgaata tgatatcaac aacgatgatt
    16981 cttctgaaaa acttgagggc ggagaatatc cagatgctga atcaggtcga acttggttta
    17041 acaactatac tgaaattttt agaaaatcaa cctctgtatc tggtacatta gatgctatta
    17101 atgtgaatgg agtcggaaat gaattaacta atcaagtagc actacgtggt atggaaatga
    17161 aaattgattt gaaccgaaaa ttgattactg gtgtaaaagc tgatgaaaat ggttctaaag
    17221 gtcgtcgaat gaatgggatt ttgaacttga tcaattcagc aaataaggca gaaacagcca
    17281 ctgcgggtgc agtaacaaga aaagatatcg atactttatt taaattgatg tatgaaaaag
    17341 gttatatggg agaaaaacta tgcttgattt ctccagatat gcaggagtta atgactgatg
    17401 agttagatgg aaaatcaaca aaaattgttc agttcgggga aagagtaact tttggattgc
    17461 aaattggaaa tatcgtgtct aattacggta caggtattgc tctactagaa ccatcattgc
    17521 caaaaggaac aattgccgca atcgatacta attatgtgaa actacgtcca ttacgtgaat
    17581 ggagagcaga agaacttgca aaaacaacgg attctagacg tatcggtctt gtaggtgaat
    17641 actctcttga atacaatgct tcaaactctg gggcaatttt aaatttaaag tctgaataaa
    17701 agggagttag tactcccttt tttgatagga ggaattacag tggtaaaaaa agatgaaact
    17761 aaaaaagatg aagtcgtgaa atatagagta ggtaaaacta aaaattttgt tgggtttgtt
    17821 catcctaaaa ctcgtagatt tatcacagca gattcaaata acgaatttat catttctata
    17881 gatgataaag aagcaattgc aattttggaa gatgcaattg atgttaatga aatttaggaa
    17941 gtgatctgat ggatgaatcg ctaaaaacgg aaatcattga gtctacaaaa gaagattttc
    18001 cagatttgag tgaagaacgc ataactaatt tattagaaat aattttgcta gaaattgaat
    18061 catacaacac ttgtaaaaat gatatttcat gggaaaagtt aaaaagcgtg attagtgaag
    18121 tgttgtataa aataataaaa aatgaatcag aaaaaacagt atcttcagtt agacgtggcg
    18181 atacaacgat tagttatgct tcaacaacaa atgatgttag tgagttactt ctaggctatg
    18241 gtgatttgat acgaagagtt attggttgtg gaggattgga gtttttttaa tgaatgaagc
    18301 agatatttta gaaactactt atgaagatag ttgtattatt gaaagactga cggacattga
    18361 agattctaat acaaatatta ctattcaaga ttacaaaaaa gtatatgata atcctatttc
    18421 ttgcgctctt tcacaaggtc aaatcgatgg actagcagtc atagaagatg gagagatggt
    18481 aaatgtttca actgacacat ataaattatt tgttcatcct aagattaaac taaaaaaagg
    18541 agatcgaata acaataactc aaaaggccag tggcttaatt ttttctctat ttgctactaa
    18601 gcctttttac tatcctagtc attgtgaagt gaatttgata ggaagtgaga aaaatgggta
    18661 atctcaaata tgaatcaaat gcagaaaaga tcattgagaa ttttaaaaat atgactgtaa
    18721 ttgctcaaaa agaaggaata tccttcgtta acgattcaat gaataaagtc gttagtttag
    18781 ctaaaccatt aactcctgta aaatcaggta atttaaggcg aggatatcgt gtagtaaaag
    18841 ctagaaaact atcaagcggt cgaattgttg gagcagttat aaacaatgaa cattatttta
    18901 aatatgtaga agaaggtcat aggactaaaa atggtggctt tgtcaaaggg aaattcatgt
    18961 taactcgtgc aacgaatctt gcaaatatgt cttatattcc tcgaagattt aaacaaatgg
    19021 caataaaaat cgttaagaaa ggaaagtaac atgaaagatg aaatcattgc tgcaatcagt
    19081 agcaaattaa aagaaatcta tcctgatggg acaatatatc ttgattcagt tatgcagtca
    19141 actaaagatt tttatttcgt tttatcagta atggaatctg gaactgaaaa tgtaggaatt
    19201 gatattcaaa atgtttcttt cttaattgat attgcattga ttgataataa acctaataga
    19261 aatttaatta atgaattagt ctcacgttgt gggacttttt ttaatacgat tacaatcgat
    19321 gagcaaatat tatttccaaa agcctattta cctgatgaag cagatggtgt tcaacatatt
    19381 cgttttacat tagaatttcc acaatatatt gaatggagtg aaagataaat gggagaaaaa
    19441 agaagtaaaa ccggaattat ttctgtcgaa aaacctactt ggtttccttt ggaagatgag
    19501 acagggaatt ttccggtata tggtactgcg ttgccaatgg gaacagcagt aagtatcaaa
    19561 ccaacagcta attatgaaac aacacaagat tatggggatt cagtggttca agatcaattt
    19621 acagcttttg gtggtgcaga agttgaactg gaagcaaatg gatatactca taaagtttta
    19681 tctacaatta ctggagggaa aattgttaaa ggtggtgcct tgcgttctgg agaagatatt
    19741 gcgcaagatg gtgcgtttgc atatagacgt aaaaaatcga atgggaaata ccgttatacg
    19801 gtcttttata aaggacagtt tgctctagat tcagatgaaa catcaacgat tgaaggaagt
    19861 aaagtaagtt ttactcatcc aacttggaag ggttcatttg tagatgtacc aggacttgga
    19921 tatatgtatt cagttgatga agatgatgaa ggcgtggata aagcaatgat cggaaattgg
    19981 tttacaaaag tagcaattcc aattgaagaa gcagaacttt caggaggtac agaataatgt
    20041 caaaatatca aacaacgatt aaattaacga aaaaagatga agaaggtaaa tatgagcaag
    20101 tacaatttaa atctgctgaa tttctaccag gcccagtcgt agaagaagca gcagaagtaa
    20161 tggagggaat gcaaaatgct gtagatggaa aatcagtatc agaagcatta agtcgagctt
    20221 attcatttat tgcagatatt ttgtttgaag gacagtttac tggtgaagat tatcgtcaag
    20281 ggatcgatgc tcgagaaatt gcttcgttga caggtaagtt attaaaatct gttactgcag
    20341 gctttgatga aacttataca gaaacgaaaa aaaagtaagc gaagctctca aatatccttt
    20401 ttttaaacat tcgattactt atcgagaatt agatttaaag acgcaattac tcgaagcagg
    20461 ttggacgtta ccagaaattg aacgtactga ctttgacgag ttaatgcgtc tttttgcttt
    20521 tcgagatgca gtaaaagagc atgaggatgt tgagtactac gataacttca cccaatttta
    20581 ggaggtgata ctttgaataa cgatgaccta attctgaaga tgatactaga tgaatctggt
    20641 tttactgccg gtatgaataa tgctgtcaaa aagttgaact cttttgatga aacaatcgaa
    20701 agaaaaagta gaaatagtgg cagttcatta ggtagtattt ggaaaatatt tgctggaagt
    20761 tttctagcta gcggagtaac tagaatcgta ggagctggct ttgatctaat caagggttcc
    20821 ataagtggag cggttgatcg agtagatacg atgaataacg ctctacgaaa tttccaaaat
    20881 atgggattta gtaattcaga aattatgaag aatatcggaa agaatgggct tttatctcaa
    20941 ggtattcaag gacttcctac tgctttgaat gatgcgataa gtcatgttca acttcttgct
    21001 tcttctacag gtgatttaac tcgttcaact caaatattta aagctttaaa tgatggaatt
    21061 cttggtttcg gtggttcaac tgatcaagtt aatgaagctg ttattcaatt atctcaaagt
    21121 ttctcaaacg gaaaagtaga cgcacaaact tggaactcaa tgatcaacgc tcaacttgga
    21181 cctactcttt ctgctattgc taaaaagatg ggaattacaa tgggagatct gaaagaaggt
    21241 ttatctcaag gtaagatttc tgttgaagaa ttccaaaatc aattaataga aatggatacc
    21301 aaaggtggcg gaggacttaa atcattaagt caaatcgcta aagattctac taagggaatt
    21361 aaaacctcta tacaaaatgc aaaaacagct gttacacgtg gtgttggtga ggtaatagaa
    21421 ggattaaata aagcattagt agattcagat ttaggtggat ttaagggaat tattgataaa
    21481 gtgggcagtt caatggaatc gttcttaaaa gtaattgctg caaatattcc tacagcagta
    21541 tcatttttaa gtaatttatt taacgaaatt caaaaatttg gttctgcttt gaaattcatg
    21601 atgccctttc ttgttcctgc agctaccgct tttggagcgt tcatgtttca acttaaaggt
    21661 ataccagcaa taataaaaag cttcaataat tttaagaatg ccataattgg cgttggaaat
    21721 tcattaaaga ttatgggggc aatagctgct gctaattcat ttgttttaat tattggtgca
    21781 gtcgttggtg caatagctgt tttcggttac tttatggcaa ccaatgaaga gcttagaaac
    21841 aaggttatat ctatttggaa tgatgtgaaa gattccgtaa ttggtgtatt aaaaaatata
    21901 aaggactggg gaattgatac ttggaattct gctaaagaaa tggcatcaaa tgcagttgag
    21961 ggtgtcaaag atgcttggtc aggaacaaag gaatggtttt ctaacacctg gaaaggtatt
    22021 aaggatgggg ccacaggttt atttgataaa acagtagaga cttcaagaaa tgcagtcgat
    22081 agtgtaaaaa atgcatggtc taatatgaag aaatggtttt ctgatacttg gcaaagtata
    22141 aaagattcag ccagagaaaa atggaatgga atcaaaggtt ctattatgtc agttgctggt
    22201 ccattgatta caggtataaa gaatgcattt ttacatgtta ctttttatct agagacctta
    22261 tggaataatt tagttgagat tggtaaaaac gtctttgaga ttttaaaaaa tgttatactt
    22321 gcgccggttt tgtttattac ctccatgatt tctggtggat gggaagaaac aaaaaataat
    22381 atgattgctg tttggaataa tattaaagaa agtgccataa at
//
LOCUS       DACCXM010000040        22176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCXM010000040 DACCXM010000000
VERSION     DACCXM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.042"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            242..2077
                     /gene="tdc"
                     /locus_tag="IVY61_001289"
     CDS             242..2077
                     /gene="tdc"
                     /locus_tag="IVY61_001289"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7970413.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2382..3860
                     /locus_tag="IVY61_001290"
     CDS             2382..3860
                     /locus_tag="IVY61_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7970414.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3826..5157
                     /locus_tag="IVY61_001291"
     CDS             3826..5157
                     /locus_tag="IVY61_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7970415.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5271..7823
                     /locus_tag="IVY61_001292"
     CDS             5271..7823
                     /locus_tag="IVY61_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP7970416.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7830..9269)
                     /locus_tag="IVY61_001293"
     CDS             complement(7830..9269)
                     /locus_tag="IVY61_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7970417.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9615..9977
                     /locus_tag="IVY61_001294"
     CDS             9615..9977
                     /locus_tag="IVY61_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970418.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10164..11021)
                     /locus_tag="IVY61_001295"
     CDS             complement(10164..11021)
                     /locus_tag="IVY61_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7970419.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11141..12052
                     /locus_tag="IVY61_001296"
     CDS             11141..12052
                     /locus_tag="IVY61_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7970420.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12059..13710
                     /gene="mdcA"
                     /locus_tag="IVY61_001297"
                     /pseudo
     CDS             12059..13710
                     /gene="mdcA"
                     /locus_tag="IVY61_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13812..14111
                     /locus_tag="IVY61_001298"
     CDS             13812..14111
                     /locus_tag="IVY61_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP7970421.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14101..15777
                     /gene="mdcD"
                     /locus_tag="IVY61_001299"
     CDS             14101..15777
                     /gene="mdcD"
                     /locus_tag="IVY61_001299"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP7970422.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15781..16374
                     /locus_tag="IVY61_001300"
     CDS             15781..16374
                     /locus_tag="IVY61_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP7970423.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16398..17474
                     /locus_tag="IVY61_001301"
     CDS             16398..17474
                     /locus_tag="IVY61_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP7970424.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17499..18368
                     /gene="mdcB"
                     /locus_tag="IVY61_001302"
     CDS             17499..18368
                     /gene="mdcB"
                     /locus_tag="IVY61_001302"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP7970425.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18479..19243
                     /locus_tag="IVY61_001303"
     CDS             18479..19243
                     /locus_tag="IVY61_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP7970426.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19732..21825
                     /locus_tag="IVY61_001304"
     CDS             19732..21825
                     /locus_tag="IVY61_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7970427.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21850..22152
                     /locus_tag="IVY61_001305"
     CDS             21850..22152
                     /locus_tag="IVY61_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7970428.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7178 a         3788 c         4725 g         6485 t
ORIGIN      
        1 tttgtttgtt cgcttttttt aggttatcta ttttagaaga ctgatttatt gaaaaattgt
       61 tcgctttttt gcgttgaatt atcagaaaaa tccgacttta tttatttttg tttgtaaaaa
      121 tctacgtgga tttgatgtta taaaaaaaaa attctcattt ttggcgctat agtgaagagg
      181 tgttacagat aaacacatgt atctgcaaga cattgaccaa caaaatatgg agggaagaaa
      241 aatgaaagat atggatatca aggccgtctt tataggcgac aaagctgaaa atggaccagt
      301 ttataaaatg ttgttgaaca agatggtaga tgaacatctt ggatggagag aaaattatat
      361 tccttcagat atgcctgcaa ttagcgaagg agataagctc actccagatt atctagccac
      421 ccgcgaccat atgatagagg tcttggatga agtcagtcag cgattacgcg caggatcgat
      481 cccatggcat tcagcaggcc gttactgggg acaaatgaat gctgaaacat tgatgcctgc
      541 tttattagct tacaactatg cgatgctgtg gaacccaaat aatgttgcat tggaatcttc
      601 tatggcaact tcgcaaatgg aagcagaagt aggacaggat tttgcttcat tattcaatat
      661 gacagacgga tggggacaca ttgcagcaga tggttctatt gctaatcttg aaggattatg
      721 gtacgcccgt tgcatcaaat cgattccttt agctgtcaag gaagtgctgc ctgaaaaagt
      781 gaaaaaaatg tctgagtggg aacttttgaa tttatctgtt gaagaaattt tggaaatgac
      841 cgaaagcttt acggacgaag aaatggatga ggtcaaagca gcttcttccc gtagcggaaa
      901 aaatatccaa agattaggga aatggctggt ccctcaaaca aaacactatt cttggatgaa
      961 ggcgttagat atttgcggag tcggcttaga tcagatggtt gctatcccag ttcaagagga
     1021 ttatcgaatg gatattaatg ctttggaaaa aacaattcgt gaattagctg gacaaaaaat
     1081 cccaattcta ggtgtggttg ctgtcgtagg aacgacagaa gaaggtcaag tagacagtgt
     1141 agataaaatc gtccaattaa gagaaaggtt aaaagatgaa gggatctatt tctatttaca
     1201 tgttgatgcc gcatatggcg gctatgcgcg ttcactgttt ctaaacgaag caggagaatt
     1261 tgtgccgtat gcttccttag ctgaattctt tgaagaacat catgttttcc accactgcgt
     1321 aacgattgac aaagaggtat atgaagggtt cagagctatt tcagaagcgg attctgtcac
     1381 gatagatcct cataagatgg gctatgtccc ttatgctgct ggaggaatcg taatcaaaca
     1441 taaaaatatg agaaatatca tttcttattt cgcgccatat gtgtttgaaa aatcagtaaa
     1501 ggctccagat atgttaggag cttatatttt ggaaggatca aaagctggag caaccgccgc
     1561 cgcagtctgg acagcacatc gtgtattgcc attgaatgtg accggatatg gtcaattgat
     1621 tggggcttca atcgaggcag ctcaaagatt cagagaattt ctcgatcatc tgacttttac
     1681 tgttaaagga aaaacaatcg aagtctatcc attgaatcat cctgatttta atatggtcaa
     1741 ttgggtattc aaagaacaag gctgtacaga tttgaacgct atcaatgaat taaatgaaaa
     1801 aatgttcgat cggtcttctt atatggatgg cgatgtttat ggtgaacggt ttattacttc
     1861 tcatactaca tttacacaag aagattacgg tgattctccg attcgttttg ttgaaagaat
     1921 gggtctaacg aaagaagaat ggaaaaaaga acagaaaatc actctgctac gtgcggctat
     1981 catgactcct tatttgaatg atgatcgaat tttcaatttt tatacaaaaa aaatcgccaa
     2041 agcaatggaa aagaaactaa acgaaatcat ccaatagaac tgatttttgt gtgaaagaat
     2101 aacaagagac atatggagaa cagacaatta tttgcttcat caaaacaatt gtatttcccc
     2161 ctgtttttca tatattcctc gccaagatat tcttttttat aaaacgttct ataagttgga
     2221 tcgttcgttt catgtatcaa acccagttca ctaagatcgt tcaatggatg tttcggtgat
     2281 actgggtttt gtactgaatc gatgaaacct tgtcatttct gttcttgaac aaaaataaaa
     2341 gaaaacaaaa acatatgtcg ctattgaaag aaggaataaa gatgaaagat actggttcat
     2401 caaagcaaat cacgaccatg acctttatcg gaatgacctg cgcccttgta gcaagtgtac
     2461 gaaatattcc agatgtagca gcaacgagct ggacgatgat tttttacatg ctggttgcag
     2521 tattattgta cgcttttcca atctcattga tctctggtga atttgctgga atgtttcccc
     2581 aaaaaggtgg tccagaactt tgggtatcga atgctctagg aaaaaaatgg gggttcgttg
     2641 tctcatggct gttatgggtc caaatgtttc ctgggatggt gatggtcgct tcagcactcg
     2701 ctccgttgtt tggtaatatt atcgataatg tgccgcttgg tttgaacagc aaatttacat
     2761 tagttgtcat tttagtcgtt tattggatca tcactttttt gaatttgaaa tttgatatgg
     2821 caaaaatcgg cggaaaagtc ggtgtttggt taggattgta cgtgcctttg acgatgatgc
     2881 tcttattagg gtttgctgcg tggatcaaag ttgggatcgt accaaccgga acattaggca
     2941 gtttttcatg ggacaaatta ataccagata ctacgactgc atcctcattt gtttattttg
     3001 cgccgatcat gtttattttt actggtatcg aaatgtcctc tgtgtatatc actcgattgg
     3061 aaaatccagt taaaacgtat attcgcggag tatttgccgc gttaattttt atatttttcg
     3121 tcaatatttt gaatgcttta gttgtagcaa atgtcgtacc aaaaggacaa atggaattga
     3181 ataatatcgc gcaatctatt tcgatttatt gccaaattct tggtttgcca catcttattg
     3241 tgaatctgtt tagtttactt gtcttcattg gtgtagcagt ccaattatct gcttgggcct
     3301 caggaccagc aaagacagtg acagaaagtg ctagaaaagg tgcgtatcca ccaaaattca
     3361 atttttggaa gacgaatcaa tttgacgtat ccaaatccgt tattttgact cagtctgtca
     3421 ttatctcaat ctttgcactt ttttatttgc tgattcctgg cgtcaatcaa gcatttttga
     3481 tgctcgtaaa ttcaactact gttatctact gtatcgtcta tgtcatcatg gggatcgcag
     3541 ttttgagatt gcgttataca catgcaaagt tgaatcgacc atttagaatt ggaaaaaaag
     3601 agaaaaagag caatcttgga gtttggactg tcgttattgt tttatttgcg gcaatcggat
     3661 tttctgtcgg actgacgatg aaagctggaa catggatcaa tctaattgca gtcacagcca
     3721 tcagtgtcat tttgtttgta gtaccactct gtattgaaaa aataaagaaa ccttcttggg
     3781 aacaggaagt cgccatgctg cttgaaaaag aaaaaggtga acttgatgga aaaaccggca
     3841 atgaaacgga ctctaactaa tcgaaatatc cagttaatcg cattaggtgg cgcaattgga
     3901 acaggattat ttttaggttc agcaaaggca attgcatcag cagggccagc tgttattctc
     3961 gtttatttga tatgcggttt ggctatttat ggtttgatgc gggccatggg tgatttattt
     4021 ttagcaaata gtacatttaa ctctatcagt gatttcgttg actattattt aggcaaaaag
     4081 tgggcatttt ttgtcaattg gacttattgg ctttgctggc ttggagctgg acttgcggaa
     4141 ttaacagcaa taggattgta tatccatttc tggtttgtac atgtaccagc tttgataagc
     4201 ggaggtagcg cactaatgat cttaatggca gtcaaccttg ttgcagtcaa gtggtttggt
     4261 gaattggaat cttttttctc aatcatcaag atcttgggga ttttcctttt tatcttattg
     4321 ggtataggat tgagtgtctc agattttcat acccagtcta tcggaatcaa agaaaatttt
     4381 ggacagatcg atgaattact accgtttggc tttgctggaa tggtagtctc atttcctatg
     4441 gttttattca gttttgctgg aattgaaatg atcggcttaa ccgttggcga gacaagtgat
     4501 ccgcaaaaga atttgaaaaa agcaatcaac tgtctgcctt ggcgtattct ctttttctat
     4561 ataggaacga ttttgtcgat tttatttgtt gttccatggc attgtttaga tttaaaagaa
     4621 agtcctttag tgactatgct gcaagtgatc cattgccaag gcggagcagc aattgtcaat
     4681 ctaatgattt tggttgcttc tttgtcctca gcaaatagtg cagtctacag tactagcagg
     4741 attttatatc aaacagcaaa agaaaaaagt ttgccccatt ggttttgtgc gctttctaag
     4801 aaaggcgtac ctttacacgg tattttaatt acaggaatgt tattttttgc cggtctttgt
     4861 ctacattttt ttgtagctga tagtcgattg ttttttcagc ttttggcagg catgacaacg
     4921 agttgttttc tatttgtgtg gtcttcgatc ttgtgtgcac atgttcaatt tattaaagaa
     4981 aagaaacgga acactacaca aagatataaa gatcttgctt tgttggtgtt ttttacttca
     5041 attgctttca gcttgttatt cgaaagcacg acacgcttta ttcctttttt attggttatc
     5101 tggtttgtat tcttaaattt tgtatataaa agaataagca atagaaatat cagttaggcc
     5161 cagatacatt ttgcaaaacg aattatttca gacccccctg aaataatcga tacgcctgaa
     5221 aaaacttccc tgcgtataat aacagtatca attaaacagg aggggatagg atgaactggt
     5281 acagattgac ggtagaagat gtattgaaaa actatcaaac atcaacagaa ggcttgtcgc
     5341 aagaagaacg acagaaacgc ctgactgatc aaggatataa tgaaataaaa gcaaggcagc
     5401 agacaaaacg atggaaaaaa atagcaaagc attttactga tctgctgatg atcgtgttga
     5461 tcattgcctc ttttttgaaa tttgcttcta gtgaatatat tgaaggaagt attattttat
     5521 tcgtagtgat cgtcaatgga ctcgttagtt actggcaaga aagaaaagcg gaagaatcat
     5581 tgaatggttt gaagcagctg atgggacaag aagcgatagt actatcaaac gggatgcaag
     5641 aaaaaatccc tgcgaaagaa ctggtaaaag gagatgtcat tgtcttacgt gccggagatg
     5701 tcgttccagc agatatccga ttaatcgaag cacatgattt attagtggaa gaatcgattt
     5761 tgacgggaga atcagaagca tctgaaaaaa gtcatgccgt tttgacagaa gaagaatcga
     5821 tcggcgacca gaaaaatatg ggtttttcag gaacgcttgt acaatctggt tctgcagtcg
     5881 gtgtcgttgt agaaacagga aacacgacag aaatcggtaa aatcaatcaa gcattgcaat
     5941 cggtcgaaca acaaacgacg cctctgatta aaaaaatcaa ccgattaaac aaacaaattt
     6001 ttcaaggcat tctttgtttg agcctattct tagttatttt tacgacattt cgatatggta
     6061 tggattggca tatccttctt tcggctacga ttgctttggt ggtgtcaatg gtacccgaag
     6121 gattgccagc tgttttgacc atgattctat ccgttggagt acatgaaatg gcaaaagaaa
     6181 aggcgatcat caaaggactt ccttcggtag aaacattagg ttctatgaca gtgatctgtt
     6241 cagataagac aggaacattg acaaaaaatg aaatgacagt tgtggatgta gctgcaaaag
     6301 aagaagcctg cgtgttgtcc attatgaaaa actgtcagga attaaaaaca aaagaagaac
     6361 agaaaacaga aaatttaagt ggcaatccga cagaagttgc gctgattcga cacacagaaa
     6421 cagccaatct acctttgaaa aaaatagaag caaagattcc gttcagttct gaatacaaat
     6481 atatggcaac gatgcatgcg gaggaaaaag gagcggtgat ttacgtcaaa ggtgcaccag
     6541 aagtattatt tgctaaatca actctttctt ctgcagaaca agaagcatgg agtcagacag
     6601 ctgcagaatt tgcgagaaaa ggtcagcgtg ttttaggatt tgcttataaa aaagtagatt
     6661 cgaaacagga actcacgcat gagactttaa cccaattaac ttttgcaggg atcgccggat
     6721 tgatcgatcc gccaaaagaa agtgcagtca aagctgtaaa ggaatgccaa caagcaggga
     6781 tctcagtgaa aatgattaca ggggatcaca aagacacagc aaaagcgatt gcggaacaaa
     6841 ttggcttaaa acatactgcc aaagttttag aagggatcga tttggatctt atgtctgatg
     6901 aagagttgat tcagcaagtg ccaatagtcg atgtctttgc tcgaacgaca ccagaacata
     6961 aactgcgaat tgttaaagct ttgcagaaaa atggagaaat cgtcggaatg accggagacg
     7021 gcgtaaatga tgcacctgct ttgaaaagat ccgatgtagg aattgctatg ggaatcaaag
     7081 gtagtgaagt aagcaaacag gcagctgata tggttttagg agatgataat ttccatacga
     7141 ttgcaaaagc cgtaaaagag ggccggcgga ttttggataa tttgcaaaaa accatcaatt
     7201 tcttcttgcc aactgcatta gctcaaggat tgatcttgat ttgggcactt atgctgaatc
     7261 gcccgctacc tttgtcccca gtacagattt tatgggtcaa tatggtaacg acaatcactt
     7321 tatcttatgc actagggttc gagtcgccag atccagaaat catgaagcgc ccaccaagag
     7381 atccaaagca aggaatccta tctggttatc atttgtttcg gatcatctat gtctccctac
     7441 tgatcatggt tcctgcctat ttattggcta tgcagtttga agggcaaagc ttacagcaaa
     7501 ccattcttct gcaaaatatc gtcttagctc aagctgttta catgatcaat tgcagagagt
     7561 tgtccaagcc gtcattgaac caaggtcttt tgcaaaacaa agcgcttttt ctttcattag
     7621 gtatccttgc tttattgcaa gggatggtta tttttctacc agtcggccag cagctgatcg
     7681 gtacagtaag tttaacgatg acacaacagt tattgattgg agcaaatgtg attcttctat
     7741 tcttagttgt agaaatagaa aaagggatca tgaacaaact gtttcgcaaa aaagaaaaaa
     7801 cagctagaga atacagtcgc tagccgttac tataaaaaag ttttgaaagt gagttcagtc
     7861 aaacgattga gctcattttt cgttggaata gaagaaatgt atacaatagg gatatctaga
     7921 gaatctagag ggaaattgga aatcaagata tctgtttttt caaaagaaat agtggaaaga
     7981 tcggttagtt cgataggtaa gaagcagaca cgttttccta agtaatcttt taattcctgc
     8041 tgcaagaacg ccttccatga aggttctcct tggaaaataa aatagacatt gaacaaattc
     8101 ggtttgatag aaaaaatcgt ttgttggatc agtagagaca agagttggag aaagtaattt
     8161 ggttcattga tttctatttc tttttttaga tcgttcagta aattctcttt ctgttgatac
     8221 aagtgtgcat attgctgatc atatgtttct aaaaaacgat ggtattgacg gtccaatgct
     8281 tcaatcaata tcggcgattc atgatatttt aatatgagga gagccaagtt atcagtcaga
     8341 gatgtttttt ttagttgatc aagagacagc tctgaagcta gttcctctac aaacgtttcg
     8401 aggacctcta aaaatggaga atgatgcagg cagcgagcgg tttcttgtcc atagttattg
     8461 ttgtaattcc aactttccag aaagcagaaa gaagcaaaag aaagctcagt gtctttaaga
     8521 taaatacctt ccgttaaata caattttttc aatgcagagt catacaggga ggagagtgtg
     8581 ctgttggtat ggatagaaga atctaatcgg caattcgctt ttatacggat cgtattgata
     8641 aagaaccaac atgttccgaa aatctcttct gtttcaaccg ctagttcagt ttgactgagg
     8701 tttcttaaaa attgaaaata atgggattca tggatcgaat aatcctcgaa aaaatagttg
     8761 ttatgagtga aaggaatcag caatcgatga taaaaagtac gaatcgcacg ctcttttcca
     8821 agaatacaag ctgtatattt cgtcaaatcg atctgcaagt cgtaacttgc taaacgctga
     8881 ttgattttag tcacatgtct tttcaatgtc ccataagaaa gcccatggtc ggctagaaaa
     8941 tcagggacag caactttttt tgtaaaaagc agatttttca gcagttcgac actgactgat
     9001 tgtttgaaaa aaatctccca aaggtcattt gttaataaat tttgctggtt ttgcaaactg
     9061 attcctgctt gatcgctgat gatgtcccag ccttcgggca gctgagagcg aatagattga
     9121 aggtcagaaa aaatcgttcg ttcggttgtc cctatgattt cagctaattc atttacggtt
     9181 atctttgtat gattcagcaa ttgttctact aaagaaacat gacgtcgaat atctttttcg
     9241 gtaatgattt ttttcattag tgaatacatc aatcagactc ccttctattc taagaatacg
     9301 gagaaaataa acggtggtca agtttcaact gatttttcaa tcattgttta tttttctgga
     9361 gcatttactg atgataagaa tttcatcatg aacttgaaaa atgataccac taaaatcaaa
     9421 aaataggtgg gcaaacaata ataaacattt taatagtaga tggtagcaag accaacacag
     9481 gcggattgaa catttaagag ttgataaaaa agaaaaatat cgaagtattt gattagcaac
     9541 tacttgtatt catttttcta ttcttacgtg gtatctttga ataaagcgaa tcaataagaa
     9601 agagatggaa gcagatgcta acgaaaaaag aactaatcga tcacatcaat gaaacataca
     9661 atttgtctcc agaatatatt tttaaaaaat ttcctgatta ttgcgtattc agacatcaaa
     9721 aaagcaaaaa atggtttggg gtcctgatgt ctgttccaga agaaaaaatc tatgatgatg
     9781 caagtgacca taggatcatc aatattcttg atttgaaggt cgatcctgaa cttggcggaa
     9841 ttttgcgagg aaaagagggg tattttccag cctatcacat gaataaagaa cattggatct
     9901 ctattgatct acgtaaaata gaaaagctag atgaactagc agatttgatt gatggaagtt
     9961 ttaaatcaac gcaatgaatg tctaggacaa tagagaagag gttgtgacag aaatgtttag
    10021 cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttggg aaatttcggc
    10081 ttattttcta agaaattact tctggaacac cgtttgttcg aaaaaagagc tgcgacaaga
    10141 attttgtccc agctcttttt tttttatttt cctaaataaa attcccgcat cgtttttgca
    10201 atcattttga tcaaaggatg atcttctgtg aagctagaag ataaataaat tgttcgtgtc
    10261 aagggcaaat ctttgatttc ccgcaagacg aaaggatcat ttttatcgta agaccaagaa
    10321 acttttggga aaaagctaac agctaatcct tgcttgacaa tagaacgtaa tgtcgcagga
    10381 tcatctgtcg tcgaactgat cgtcaattcg atacttttcg cttcaagtgc tgcatccagt
    10441 gtatgacgga aggcgttgct ctttgacagc atcaagatgg gggtgcgttg gatatcttct
    10501 aattgcagat gttctttttt agcaagagga tgtttcttag gaatggcgac ataaacaggt
    10561 tctttcatga taggaatatt ggtcatgcca ggcttcatct ccgaagtgat gtacagtaca
    10621 ggatcggtaa tatcatctgg taaatgatgt tgcatgatcg ttaattggat atcaggatgc
    10681 ttttccataa aaattcggac caagttcgga atagaaacag aggctacctc taaatacaat
    10741 atgacggaag taacgatctt ctcattgacc gaagacaata atttgattcc ttcttctaat
    10801 gtgtctaatg tcttgactgc atatggataa aattcttctg cataaacggt cgtctcaatg
    10861 tttctgccgc tatggtaaaa caaagggaca cctaattcgt tttccaaatg tttgatcgca
    10921 cggctcaagg caggttgaga aataaataat tctttggctg cttgggtaac attttttgtt
    10981 tcgaatactt ttttaaaata ctgcagatga agcaatttca taattatcca tcccatccat
    11041 acctttttgt tatggttttt tctttttttg gcatttttta ttatagcaga aattgactaa
    11101 gatagcagtg gaaaaagtaa gtgctgagga ggcgaggctc atgagtacag gtttcttatt
    11161 tcctggacaa ggcagtcaga agttagggat gttagcggat atccctgaaa tgtacatgga
    11221 acgtttagaa aatgtgacgg ggtatcggtt agttgataaa gaagaaaact atcaagatac
    11281 ggtttttatc cagctagcat tactgacaaa agcagtattt tatttagatg aaatggaaaa
    11341 aaacggtatt cagcctgata ttgcagcagg acattcgatt ggtgcttttt gtgcagctgt
    11401 tgcttgtcga tctttgagat ttgagaatgc agcggaacta gtctatcacc gcgcagtctt
    11461 aatgaaagaa gcttatccaa gcggttatgc aatgggcgta gtagtaggtg tgacgagaag
    11521 cgaagctgag gaaattgtag caaaaacatt tgatcctgac catccagttt atctgtcaaa
    11581 tgaaaattgt ccgatgcaac atacgttatc tgggagtatc gaaggtttat ccgttacgtt
    11641 ggcagaagcc aaacggcacc aagcgcaaac agcgaagctg ctgaaagtcc ctgtgccatc
    11701 tcactgtctc ttgatgaatg atacggtcaa gaaattcaag ccgtatatgc agtctgtcca
    11761 tctatctgat gctagatgtt tgtatataaa aaatacggat ggaagagcca ctcaggacgc
    11821 agaagagatt cgtaaagatt tgttggaaaa cctagctttc cctgtgcagt ggaaccaaat
    11881 gatggacttg gcaaaagaat taggaatgga tctgacgatt gaatttcccc ccggcaatac
    11941 tttgacaaag ctgattcata caaaatttgg tgtagataaa gggatccgta cgatcaatct
    12001 agaccaacat ggtattgatg acgccacgtt tctatatcaa aaatggagat gactaattat
    12061 ggaaaaacca aaccgcaact gggcttctaa acgcgaaaaa aaagcgcagc gattggaaaa
    12121 aatcagcaac ttgctcaatg gcaagtatgt agaaactgaa aaaatcgtag aagtaatgga
    12181 aaaactgatt gctcccggcg acaaagttgt attagaaggc gacaatcaaa aacaagcaag
    12241 ttttctttca gaaagtcttg aaaaagtaga tgcagaaaaa gtgaatgatc tgcatttgat
    12301 catgtctagt atctcacgac cagaacattt gaatattttt gaaaaaggaa tcgcaagcaa
    12361 gatcgatttc tcttatgctg gtgcacaaag cttgcgtgtc gcccaaatga ttgaagacgg
    12421 cacaatgaaa ttaggtgata tccatactta tttggaactt tatggacgct tattcatcga
    12481 tttgattcca aatgttgttt tagtagctgc agataaagca gataaagacg gcaacttgta
    12541 cacaggtttc aatacggaag aaacaccaac aatcatcgaa gctgcagctt tcaaagatgg
    12601 tatcgtgatc gttcaagtta acgaagttgt cgattcttta ccacgtgtag atatccctgg
    12661 tgactgggta gacgttgtcg tgaaagcaga taaaccttat caattagaag ctttattcac
    12721 acgagatcct caaaacatca cagaattgca aattctaatg gcaatgatgg caatcaaagg
    12781 aatctacgca gaacacggcg tacaatcttt gaatcacggt attggattca atacagcagc
    12841 tatcgaattg ttactgccaa cttatggtga atcactaggg ttaaaaggga aaatcgcgaa
    12901 gaactgggta ttgaacccac atccaacgat gattccagcc atcgaatcag gttgggttga
    12961 aagcatccat agttttggtg gcgaagttgg tatggaaaaa tatattgctg ctcgcccaga
    13021 tgtctttttt gtcggtaaag acggcacgat gcgcagtaac cgaacattat cacaagctgc
    13081 aggacaatat gccatcgata tgtttatcgg ttcaaccttg caattagatt acgaaggaaa
    13141 ctcttctacc gtaacaaaag gacgtttatc tggttttggc ggtgcgccta acatgggaca
    13201 taatcctggc ggtcgtcgtc attctacacc agcatggcaa tcacttcgcg acaatgatga
    13261 tccattaggg aaaggccaaa aattagttgt tcaaatggtt gaaacatatg gttcaaataa
    13321 aaaaccagtc tttgtcgaag aattagatgc actagctgtt caaaaacaag caggcttagc
    13381 caatgcaatg caccagtgat gatttatagc gaagatacta cacatatcgt gacagaagaa
    13441 ggaattgctt atttgtacaa agcaaaaaca aaagaagaac gacaagcagc aattgcagca
    13501 attggcggtg ttacacctct tggaatgact agcacgaaag aatcactaga tagcttgcgt
    13561 gcagccggca tcgtgaaatt accagaagat ctaggcatca aacgtagtga agcgaaacgt
    13621 tcattattag ctgcacaaac catcgatgat ttgatcgaat ggtcagatgg cctgtatgaa
    13681 ccaccaatga aattcagaac ttggtcataa aattggcgag aaaaaccttt tgtcgccatt
    13741 tacccagatg gaagaagaaa gacgaataag cagaacgtgt atgctctgtt ttacaaaaag
    13801 gggagagatc catggaacaa ttagcttata aatatccaac gaaacagccg atcaataaga
    13861 aaatccatgt aggagtggtc ggttcaggag atttggaaat cttgatgttt ccaacagaaa
    13921 aaacagaatc tactgtaaaa atttgtacag gaagcgatgg cttcggcgaa gtatggaaca
    13981 atgtattaac gagatttttt gatcgttatc caatttcagc agatatcgtg atcaatgact
    14041 tcggtgctac accaggagtt gtgtacctac gattgacgca agcaatggag gaactttcag
    14101 atgaaaaata gttttgtaga attaaacgcc agagaacgag tgaacgctct acttgacaat
    14161 ggacgtgaat tgattgatcc attcgatcaa atcatcgcgc caagtttacc agcacaagga
    14221 atcgtccctg aaagtgatga cgggatcgtt gtcagccgtg gcacgatcaa tggcaaacaa
    14281 gcagtagtaa tcgctatgga aggaaaattc caaggtggcg gtatcggtga agtttccggt
    14341 gcaaaaatcg ttgctgcact tgaacatgtg ttagaagaaa ataaaaaagg aaatgagatc
    14401 tttccagtca ttgtactaga tacaggtggt gttcgtttgc aagaagcaaa ctacggactg
    14461 ttatctattt cagaaatcgg aaatgtaatc attgctctaa aacaatatgt accagtcatc
    14521 ggtttgattc caggacgtgt aggatcattt ggcggtatgt cgattacttc agcattaatg
    14581 agttatttaa tcgcaacaaa aaaagcgaga gtcggtttga atggaccaga agttattgaa
    14641 caagaagcag gcgttcgaga atttgattct agtgacaaag acttgatttg gaatacatta
    14701 ggatctcgtc aacgggtaga atcaaaaatc attgatgaat tagtgactga tagtgtagaa
    14761 gcaatcaaag ctgctgtcat cgcagcaatg gacgaacgaa aagacacgca tcgcagtgaa
    14821 agctcagcat tttatttatc attacttgat tctttagatc tttcaaaacc aatggccatt
    14881 gacgattaca atgaagcaat tgctaaccat caagcaaaat caattgactt accagaagta
    14941 gcagaaggaa atgaaccagc tgcagcaagt gttggttacg attggtttgc ggcactaact
    15001 ggcatgaaaa atccaacttc ttctattagt actgtgtatt atggtaaatc aagtaaattc
    15061 atagaagaag ccatcgtgac atcaatcgta cctgatccta aaaacccgat gtaccgtgta
    15121 cgaaacggtg aagttggttt agttgaaggt ttccgcatgg gacaaatttt ggatcacgtt
    15181 tacgaagaag ataaagacaa aaaagtgaaa cggccaatca tcgttgtgat cgatgtacca
    15241 agccaagcat acggctataa cgaagaattg atcggtatcc atgtggcttt ggctaatagt
    15301 gctgctgctt atgcaaaact ccgtcaagct ggacatccag ttattggatt gattgcagga
    15361 aatgctattt caggtgcatt tttagcacat ggattacaat caagccgctt gattgcttta
    15421 aacaataaag aaattaccgt ccaagcgatg tctaaagaaa gtgctgcacg tgtgacaaaa
    15481 cgttcggttg ctgaaatcga agcagctgct gaaaaagttc cagctatcgc ttacgatatc
    15541 aataattata caaaactagg agcgatctat catttcctag aagatatcac cgaccaagct
    15601 gcttcagaac aaaatgtcag caaagtcatc gaagcaatca atgaagccat tcaagatgtc
    15661 cgcaatacaa atgacacgat gctgacaaat cgatacacaa acgaaacagc caaaacattc
    15721 ggacgtgtcg aaacaaataa agtccgtgcc aaaatggacg aagaatggga tgcataaaaa
    15781 atgattcaag cacatgatat tgtccttttt gataaacatg aaatagaagg gttcaccttg
    15841 cctgaatggg tcgttctaac tgaaaagatg ttcggaacag ttcgtcgtgc gtcgacggat
    15901 cacgcagaaa tactaccgat tggtttacgt ggacctgccc gcaatcagag atggtcaaca
    15961 gaaatctcaa aacatgccgt caaacaagtc attcatccgt gggacatcat cgaacaagaa
    16021 tcgtatagac aagagaaaat catggattat cctgtttatc agcaatttca tgctgctaga
    16081 aacatattat caggatgtaa atggggaatc ggcggcagct taggatttga attatcgact
    16141 gggattccgg cagtaaaaga aaccagtgat tttgatttgc tgctgtacgc aaattcaccg
    16201 attgaattgc caattcaggt aattcaatcg catcctgctt tttttgagca attcgatacc
    16261 caagtagtga ctcggcaagg cggtttttca ctaaaagagt atttgagaga gccagagaaa
    16321 aaaattctat tgaaaacagt cgaaggacct aaattaacca aagaactttg gtaataaaga
    16381 aaaagagaga gaaagagatg tttgaaaaca aaaaaatcaa aatcaacgta agtgacttcc
    16441 agaaagtatc agctgtaacg gctgttatga tgcaaacgat attgagcttt gctttggcaa
    16501 acagccacag cgcaacaaat acagcttttc ttggtacctt ttacgtcatc gttaaataca
    16561 cagcaccaat gtttattttt gcaattgttt ataatatggt aaaaacaagt cagcatttgt
    16621 cttattggga atttttgaaa gaaaagtttt ttgaactagt cgttccgtat cttctatgga
    16681 cgacggctta tctttggcta tttccagcag tccaacaaca aactccgtat acggatactg
    16741 caagcttttt actgaaatat gtcacaggtg atggagcgcc tcatttatgg tataccgtta
    16801 tgatgctgca gatccaattg ttcatgccgt tttttgtttg gctcggttac aaagtatttg
    16861 ctaataaaaa atatgtatgg ccagtattga ttggttcaac gattttatat gtcgcatggt
    16921 atctgttcta tcaaacacaa gtattgactg ggccttatca tgacagctgg tacttattag
    16981 atcggtttgt tgctagtttc atgatttatg gggtagccgc atttgtttat cacgaaaaag
    17041 tctatcaata tttggacaga gtacgttatc ttttcttacc tgttgcctta gttatcgcgt
    17101 tcttttctgt ccgttcattg ctcgcacatc caggagactt gagctttgcg aatgcacctt
    17161 acttgaatac gatccaaagt ttatacagtt tagtcatcat ctttgctgtc ttcataggcg
    17221 cttccaaaat gattgtaaac gattcgccaa aattgccatt gttcaaatgg ttgtctgttt
    17281 atgcttatcg tacgtattta gccaatgtat ttgttttcca agtactgtta ttattgttta
    17341 aagatagttg gctgcagtta ccttcaggag tcatgattct tgtagcttat ctaatgacgg
    17401 caagttgtgc atttgcttta agctggttac ttcatatcat ttgggtagct atcaaaaaag
    17461 gtttcagcaa gtaatagtag gaagaaggag tgagtgtcat gataaatgac gaacgaaaaa
    17521 aagcagtgac tcagcaactc gctgcgttgg tagaggaagc attgatcgca gaagtaacat
    17581 taagtccaaa acctggatta gtggatgcac tggatgcggg tgcacattcc gatatggact
    17641 atgctttatt tcttaagtct gctaagacgc taactccttt ttttgaagaa atggctcaaa
    17701 tagcatggca tcatgctatt gatcaggagt taagagaaag aattgctgag attgggcgaa
    17761 aagcggaaaa ggcaatgctg attgctacaa atggcatcaa tacccataaa ggagcgattt
    17821 gggcaatggg gctattgatc agtgtcattg cacaaaaaat cagtatggaa cagccttttt
    17881 ctttatcgaa aatacttgat gatgtcagca agcttgctgc ttttcctgat acaaaatatc
    17941 gtcataaaaa aaccactcat ggtcataggg tgaagcaaaa atatggcgta aatggtgcat
    18001 atgaagaagc gatattaggg tatccgcata tcaggcttgc aattcaagca gctaaggagc
    18061 tagataatga ttcatatgta cagaaacaaa tgcatatgtt gttagttttg atgagttcgc
    18121 tagatgacac atgcgtattg catcgcagtg atcagaaaac gttagtggaa atgcaaaaac
    18181 ttgctcatgc tgctagtcaa agtgctttac caaatcgtcc atttacagca ctgttgcact
    18241 tttgtcagga aaaccaaatc tctcctggag gaagcgcaga tctcttgtct gcaagcttgt
    18301 tcatgctgaa gatagaggaa ttatggactg ccgacacata catttgtccg atgttactca
    18361 atgactaaat aagacaaaat tgctaaaaaa acagtttatg catctggtta ttgactcttc
    18421 cttttagcaa ggctatcata aaaatagttt tttgtaataa aagataacga ggaagacaat
    18481 gaaaaaaatc aattatttag caatcatttt gttagtgggc ctatgtttgt ttgctctgcc
    18541 gacccaagga ttcgctgtcg cgggtggtca tggtggaggt acatctagcg gtcgaagttc
    18601 gggcagaggt tcatttggtg gtcgttcata tgggggcagt tattttggca gtcgttctta
    18661 cggcagagat tcttatgggt attatggtcc aggtacaggt ccttctagtt atgagcaaac
    18721 gggatttctt gcattagctg ttttgtcagt aggaggggtt tctgcttttt ctatcgtcaa
    18781 gaataaaaga caaaagaaaa ggctgaaagc ggagatattg tcccgcatgt caggaacggc
    18841 taaagaaaag aaagcacagt ttctggaaat ccaaaagata ttcatgaaga tccaatccgc
    18901 ttgggaagaa gaaactcctg aaaaagccag aagctgttat acagacaagc tgtttgccga
    18961 acatcagaaa gttctgcaaa aaaacaaagc agacggtgta agaaatcata ctaaaaaaat
    19021 aactattcag gaagtaggaa attatcgaca gatccacgaa aatagttttg ccatccgaat
    19081 tgatttcaga tgctgtgatt attcagtcga tcgaaaagat gggcgattga tctcaggaag
    19141 taagacacaa cgacagtatt tttctcagat atggtatttt gatttttctg agaaagaaaa
    19201 aagatggcaa gccgatttta ttcagccgat tatgctggat tgacaggaaa tatttatgaa
    19261 cggagttgtg acaaaagcct tgtcgcagct cctttttctg aataaacggt gtgccagaga
    19321 tagcctatcg gcaataaggc acgattttgt aaaatatggg aagcgatttt tcaaaatcgc
    19381 ttcttatcgc tcaaggctga acatctctgt cacaacctct ccactaaatg gtgtttaaaa
    19441 gcttgcatct gcgataataa gaacaaccaa acgaaaaata ggaagcacta ttttagttta
    19501 gttgttctta ttacttgatg caggacagct ttttcacaac ctcttttttg tatagatggg
    19561 caaaattttt gtaataatag gcgtatgtct ttttcgttga ggagatgaaa aagcatatgg
    19621 cttatttttt gtttgctttg cttactttct ttccacttca agtggcagaa atatctactt
    19681 tttattatgt aagcggaacc atatacttga aatcagaagg agatcatttc tatgaaaaca
    19741 agaacaattg aaatacttca cagaatcatg acagcccaat atccagtaaa agcaaaacat
    19801 ctagcaagtg agttcaatgt cagtcagcgg acgatccgtc aggaagttct tgaagcgaat
    19861 atctggttga gcagtaaaaa gcttccggag atccgtacaa ttcgcaacaa aggttttttg
    19921 ttgaagctgg aaaaggaaaa gcaagctcga ttagaaactg aaataaaaga tgtgaaagac
    19981 ggctttttga atcggcgcga acgtattttt gatctagttt tagccgttgc ttacagccga
    20041 atcccgatcc atctaaacaa aaaagaagaa gaatttcaaa tctctaaaag tacattagat
    20101 gaagatatga gacggttacg agcaacactg gtcagttatg ggatagagat cgtcagttat
    20161 ggaaagcaag gactggttta taaaggttca gaacgatcta tccgtacgat gatctatgat
    20221 tttatcaatc agaatctggg aatgaccgac ttctttatga atcaagatgc acaattaaca
    20281 ccggctcaaa aaatctttct ccattatttt cctttggtgg aaatcaaaaa aatacaggag
    20341 atctattcca ataagttgtt gaaacaagaa gatgatattt ataaaaaaca aatcctgctt
    20401 tttacattga tctggatcca gcgaatcagg aaacatgaat ttatctctgc cattaattgg
    20461 aaaaatacag aaataaaaga cagtagcttt tctgcattta ttgaccacat gatcgaggaa
    20521 tttcaattgg acaatgtccc gcaagtagaa agaaattatc ttcgtttcac tattgagacg
    20581 ttcaatgcta gagatatctc caattcaatc gagtgggtac aagctcaatt actaacgatc
    20641 caattgatcc aatttgtaga gaacgagaca cacgtacctt tccatctaaa ggaagaagct
    20701 ttattcgaaa atctctataa gcacatggct gcactgattg taagaatcaa gaatcatatc
    20761 caagtcatga atccattgaa agaaaacatc cgaacaaatt atttcccaat ctaccgcgca
    20821 gtcactcatt ttgttcctac gatcgaagaa gtaattggtg gcaagatact ggaggacgag
    20881 atagcttttc ttgtcatcca cttttcgacg atcgccagtg taatcaagcg agacttgaat
    20941 tatgtttata aatcagtcgt tgtctgtaac catgggatgg ctactggaaa tctgttagca
    21001 gagaatctga aagaaaagtt tcctcagatc gagattactg cagttttaag ttcaaaagaa
    21061 atcgaattag tagataaatt ggatgtcaat ctggttttca gcacctttca attgaattat
    21121 cgaaataagc cgcttttagt tgtcgatccg attttaactg agacgaatat cttggttata
    21181 gaagactttt tgaaaagaaa tgaagaacta cagcggttag agcctaacag tcatgaatcg
    21241 acggagttat tcactcaaat aatagatgtc atgaaagaaa gtggtggccg agtagatcga
    21301 atcatctatg gcaagttaga aaaattattc gaaacaaaca atttagaaat caacaaaagg
    21361 gagatccagc ctatgttaaa agatattttg acggacagca atatcatgat caaagaaaaa
    21421 gccactactt gggaggaatc gattgaacta gctgctagac cattggttaa agaaaacatt
    21481 gtcgaagagc gctatgtcga agcgatgatt cgtgcagtga atgattacgg accgtatatc
    21541 gtgattggca aacacatggc actagcccat gcccgaccag aagatggggt caataaatta
    21601 ggagtcagcg tagctacgat tgagcaacca atcgatttcg gtaatcctga aatggatcca
    21661 gtaaagatta ttttttgttt ggcagcagta gactcctact cgcatctgaa catcatgaaa
    21721 gaactaattg aattgatcaa caacgaagag aagttggatc gattgattga atgcacaaat
    21781 atcgaagatt tcaaacaact gcttttttca gtagttgaga aataataaat gatacggggg
    21841 agaacaaaga tgaacaaatt gaacatttta ttcgtatgcg gtgcaggttt aggaagtagt
    21901 tttgcagcac agatgtctgc tgaggatgtt ttgaacaaat taggagtaga agccaaactg
    21961 gaccattgtg atatttcttc cgcagtttcc atgaaaccag atgtgatcat cacagcagaa
    22021 aattttcgtt cgcagttcga aaagttcgca gttgatccta aaacggcgat gatctatttg
    22081 aaaaatattg tttcaaaaaa tgaaattgaa gaaaaaatca ccccagttct gcaagataag
    22141 ggagttatct aaatacaaaa agaaaagagg gtaaaa
//
LOCUS       DACCXM010000041        22160 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCXM010000041 DACCXM010000000
VERSION     DACCXM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.043"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..196)
                     /locus_tag="IVY61_001306"
     rRNA            complement(<1..196)
                     /locus_tag="IVY61_001306"
                     /product="16S ribosomal RNA"
     gene            116..259
                     /locus_tag="IVY61_001307"
     CDS             116..259
                     /locus_tag="IVY61_001307"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970429.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(877..2373)
                     /gene="lysS"
                     /locus_tag="IVY61_001308"
     CDS             complement(877..2373)
                     /gene="lysS"
                     /locus_tag="IVY61_001308"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP7970430.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2484..3488)
                     /gene="dusB"
                     /locus_tag="IVY61_001309"
     CDS             complement(2484..3488)
                     /gene="dusB"
                     /locus_tag="IVY61_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP7970431.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3489..4376)
                     /gene="hslO"
                     /locus_tag="IVY61_001310"
     CDS             complement(3489..4376)
                     /gene="hslO"
                     /locus_tag="IVY61_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP7970432.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4593..6704)
                     /gene="hflB"
                     /locus_tag="IVY61_001311"
     CDS             complement(4593..6704)
                     /gene="hflB"
                     /locus_tag="IVY61_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP7970433.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6794..7339)
                     /gene="hpt"
                     /locus_tag="IVY61_001312"
     CDS             complement(6794..7339)
                     /gene="hpt"
                     /locus_tag="IVY61_001312"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP7970434.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(7339..8784)
                     /gene="tilS"
                     /locus_tag="IVY61_001313"
     CDS             complement(7339..8784)
                     /gene="tilS"
                     /locus_tag="IVY61_001313"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP7970435.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8904..9374)
                     /locus_tag="IVY61_001314"
     CDS             complement(8904..9374)
                     /locus_tag="IVY61_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP7970436.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9420..9845)
                     /locus_tag="IVY61_001315"
     CDS             complement(9420..9845)
                     /locus_tag="IVY61_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP7970437.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9912..10181)
                     /locus_tag="IVY61_001316"
     CDS             complement(9912..10181)
                     /locus_tag="IVY61_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP7970438.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(10192..11787)
                     /locus_tag="IVY61_001317"
     CDS             complement(10192..11787)
                     /locus_tag="IVY61_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7970439.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11802..15323)
                     /gene="mfd"
                     /locus_tag="IVY61_001318"
     CDS             complement(11802..15323)
                     /gene="mfd"
                     /locus_tag="IVY61_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP7970440.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(15340..15900)
                     /locus_tag="IVY61_001319"
     CDS             complement(15340..15900)
                     /locus_tag="IVY61_001319"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP7970441.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            16253..17227
                     /locus_tag="IVY61_001320"
     CDS             16253..17227
                     /locus_tag="IVY61_001320"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7970442.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17397..18797
                     /locus_tag="IVY61_001321"
     CDS             17397..18797
                     /locus_tag="IVY61_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP7970443.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18875..19597)
                     /locus_tag="IVY61_001322"
     CDS             complement(18875..19597)
                     /locus_tag="IVY61_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7970444.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19676..20182)
                     /locus_tag="IVY61_001323"
     CDS             complement(19676..20182)
                     /locus_tag="IVY61_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7970445.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            20305..22104
                     /locus_tag="IVY61_001324"
     CDS             20305..22104
                     /locus_tag="IVY61_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP7970446.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6132 a         4832 c         3983 g         7213 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttgtt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctact tcatcgagag tgtttcccga
      361 ttcatttttg gctgccgtag caacttttat atcatatcag agagacaagc gattgtcaac
      421 tcttttttaa aagttttttt cgatttcgtt tttcgaaatc tttcggcaac tcatttatca
      481 taacttatcg tttgttattt gtcaagaact tttttgaagt ttttttcgat agaatcaagc
      541 ttcgcaagtg gttctttttt aacgactcag ttatcttatc atgtcgttag taacatgtca
      601 ataacttttt tgttatgttt ttgaataaat attttcttct gtcttttgga caagtaataa
      661 tataccaact aaatttagct tggtcaacga attttttgta ttttttcaag tttttttcaa
      721 taaacgatta ttcataactt ttctttcttt atcgttcgtt ttcgtcttct ttaatgtcta
      781 gaatagtcgg cattagcttt tgaactttcg ttattattta ttcctttcta cacagcaaaa
      841 gctgcctaag gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa
      901 aacatcccga attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc
      961 tagaccacca gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc
     1021 gtgagcttcg tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg
     1081 atcaattgga tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag
     1141 ttcgaatcga tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc
     1201 gactggatgt ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa
     1261 gaattcattg ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc
     1321 ggctaaagct aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc
     1381 tttgattgca tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc
     1441 atcatatgtg atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc
     1501 tgtcagattc attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa
     1561 ttctggatta tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac
     1621 tttttccatt ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata
     1681 taaatcaatg tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc
     1741 ctcattgtgc aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg
     1801 gatttcgctg ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat
     1861 taaatccaag tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc
     1921 tggcagtgga cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc
     1981 gcctgtgtct gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg
     2041 tttgaatact tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg
     2101 gccttcacga tcttgtagat gcgcaaatcc agctttccct tttcctcggc gagtcatcat
     2161 acgaccagct acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc
     2221 aaatagttca tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc
     2281 gattccatct tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa
     2341 atcttctact tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc
     2401 tctttcctat aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg
     2461 aaaattcacg aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt
     2521 tctacaaatt gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct
     2581 actttcactt tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat
     2641 tctctacaag ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct
     2701 gtagcaattt tttcggctgg tgttggttca ggaatcagtt cccctgtttc gagataatgc
     2761 tgtgtgcggt gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct
     2821 gcaccgacat agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc
     2881 ataaatggaa tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct
     2941 tcatacatct gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt
     3001 gctgctaatg cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg
     3061 attgttactg ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca
     3121 ggatctaaaa gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc
     3181 atattgatat ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact
     3241 agactatctt tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg
     3301 tggagcatct ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca
     3361 catacgacta agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt
     3421 ccagccatcg gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg
     3481 tgttccactt aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta
     3541 tacttgtatt tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc
     3601 attgcttgga tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa
     3661 cagtcacatt tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc
     3721 aatcctttca atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt
     3781 gtcgttttta attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt
     3841 tggatcatga atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt
     3901 ccaacagctg aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt
     3961 tctccagaaa caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg
     4021 acagtaaaca ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt
     4081 agaggaagac tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca
     4141 tctactacaa ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct
     4201 cctttgagcg tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag
     4261 gaggtgtgcc aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg
     4321 gcgtatgctc ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt
     4381 tatgtactcc tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt
     4441 ttcaactttg gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca
     4501 ccctatttac aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg
     4561 gagtcatatc agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta
     4621 tcttctgatt gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt
     4681 tttgcttctt caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa
     4741 gcacgttttg cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca
     4801 gtaccttcag gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt
     4861 tcgaattcca aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt
     4921 tctcgagctt tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa
     4981 gcgacttgtt ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg
     5041 tttccttcat attgaacagg acctaaacga tcactcattc catattctgt taccatgctt
     5101 cgtgctagag ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag
     5161 atgatttctt cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct
     5221 ttcgtcatca agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg
     5281 cgagggatga tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc
     5341 gtatgaccag cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct
     5401 tttttagctg gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt
     5461 ttctttttat ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct
     5521 gctccggcaa aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc
     5581 ggtttattcc gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca
     5641 actaaaatct ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa
     5701 cggttggttg cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca
     5761 agcaattggt tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt
     5821 tgacgaccta ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct
     5881 gtttcaaaca aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca
     5941 gaacccgaga tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag
     6001 gttttacctg ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat
     6061 tcaacaaatc ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct
     6121 tcttccgcac ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg
     6181 gcttttgatt taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt
     6241 cccatcatca tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg
     6301 gaaacccata taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt
     6361 gccagttctg ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg
     6421 gtagtagtcg tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc
     6481 tgttctttgt attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct
     6541 ttgatcttgc ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc
     6601 tggccgttat taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca
     6661 taatacaagg catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag
     6721 cgtcaattta atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa
     6781 ttgagataca gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa
     6841 ggttacgata tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt
     6901 tgaagccaac ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg
     6961 taacaatttt gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg
     7021 ttcttccgct gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat
     7081 ctaagtcttt gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa
     7141 catccatgaa atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg
     7201 gtaccgctcc ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta
     7261 actctttgcc tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct
     7321 caatatcctt ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag
     7381 aagtatgtag tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg
     7441 tatcaagcta tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga
     7501 attaggtact ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa
     7561 tttaatgcgg tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca
     7621 aatgtcatga gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg
     7681 tttgaccaga ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa
     7741 caagcgctgg tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa
     7801 atgatactct cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt
     7861 ctgttgccac tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt
     7921 tgagaatgct ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc
     7981 ttcaatcatt tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat
     8041 caactgtccc atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg
     8101 ctggaaatgt ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg
     8161 aagccgattt ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg
     8221 cgattccgct gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag
     8281 tctgcccgta gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc
     8341 acgaacgatt ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa
     8401 cgtgtcatat tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc
     8461 ttctgtttct atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt
     8521 cttctcttca caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg
     8581 gtgattgatg tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa
     8641 atggagcaaa accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg
     8701 ttgccagaat ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt
     8761 tttcccgcct ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc
     8821 gggaaaacgt ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc
     8881 gcactatcat tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct
     8941 tctgtgtttc gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc
     9001 aatgaatcga agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt
     9061 gctggacgat ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct
     9121 tctccagatt cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca
     9181 tcatttactg aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg
     9241 tcttttacat agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct
     9301 agatcaataa acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct
     9361 acctcgattg acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct
     9421 tattttgcag ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt
     9481 gattctaata taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca
     9541 agtttagcta catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg
     9601 tctgcgttga cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc
     9661 atcctttgca tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca
     9721 acaaaaattg cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat
     9781 tcagcatact ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc
     9841 gtcacttgga ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa
     9901 gcttgtctaa tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt
     9961 ttttgtgcat cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt
    10021 ttgttgccaa actggacttt caaaatatcg cctactttga catcactgga agatttcgcc
    10081 aatttaccgt tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt
    10141 ttgatgattc gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt
    10201 aattttttta tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac
    10261 tgtaaacaga cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc
    10321 tatcacgctg acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa
    10381 tacaccataa tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg
    10441 tccaaaatgc cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata
    10501 ccaaagcgtc acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa
    10561 accggtcaga aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa
    10621 agaagggcga aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt
    10681 gaaagcaatg gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa
    10741 cgcataattg atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc
    10801 ggtcgtcaat ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt
    10861 caaatgacga gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc
    10921 taattgtact aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc
    10981 tgacagtccg taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa
    11041 gataagcaat ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa
    11101 gggctttgac ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat
    11161 cttttgttca tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact
    11221 cattgctgcc gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact
    11281 acagacaatc acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc
    11341 catcaagaat cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa
    11401 ggtaaaggaa actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat
    11461 ccagctgcta aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg
    11521 ataaagattt tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt
    11581 tgatatgaac tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata
    11641 aacttgctga taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta
    11701 aagggcactt aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat
    11761 gacgcgccgc atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt
    11821 tgctcacgca acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt
    11881 tggttgggga tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt
    11941 agttttgtcg ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact
    12001 ttacttagag taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga
    12061 tcgccatcca ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct
    12121 ccaaaacggt cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca
    12181 cgaattcgtt tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga
    12241 tacgcatcga caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct
    12301 tgtttacgag aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca
    12361 ataaatccat gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc
    12421 atcgcaatct tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt
    12481 tcactgactt cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg
    12541 ctgcttcttc ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg
    12601 ttctcaacga acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta
    12661 gtgacaagaa catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc
    12721 tctgtcattt gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct
    12781 tctacttttt gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt
    12841 cccatttctc gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt
    12901 tggatcggat aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac
    12961 atcgacatat gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt
    13021 gcacgtaact gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg
    13081 atcagtagac ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca
    13141 acaatatcaa cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt
    13201 ctgaaacgac ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt
    13261 tcatagtgct gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc
    13321 tttatcgctt taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct
    13381 acgagtagtc gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt
    13441 ctcaactggt catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg
    13501 tcatcaggtc caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga
    13561 tcgtcagcga tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt
    13621 ccgcctagct tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga
    13681 ttcagttgag taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag
    13741 tcttggtgaa cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga
    13801 ttggcatgga caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg
    13861 tttgttactg tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct
    13921 gtgatagcta ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact
    13981 aattgtacct gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca
    14041 tctcgaagca tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca
    14101 gtctgttttt gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag
    14161 aactgttgca tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc
    14221 cctttttgga agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt
    14281 atatctgctg aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc
    14341 cattctgccg cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca
    14401 acgactagta aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga
    14461 agatctgagt agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc
    14521 tgaccgaagt aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt
    14581 ttttctagca ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact
    14641 tgttcactcg ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct
    14701 gcttcaaaat accgcataga atctatctct acatcaaata gttctacacg aacaggatag
    14761 gttgtcgtta acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt
    14821 gcgaccatct cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg
    14881 tctatctcat caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc
    14941 agtcgtttgt gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt
    15001 gcgttaagcg ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca
    15061 gaaagcactt catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga
    15121 tcttctgcta actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt
    15181 gttttcttaa acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt
    15241 agttgtctag gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt
    15301 ccgactaatt gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc
    15361 atccacgaac gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct
    15421 ttcttctatc aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt
    15481 ttttttccct tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa
    15541 atgggaaata atacttttga taccgttatg cccgccggca cttccctttt gacggagccg
    15601 gatttttccc actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat
    15661 cccaaaataa gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg
    15721 tttgactaaa aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg
    15781 attttttttg aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat
    15841 attgtgtttt gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat
    15901 ttttgttttc cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc
    15961 cagtccatcg agcttttttc ttttttacac ttgtcaatgc atagtataac acaaaacaag
    16021 gcgtttcggc taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag
    16081 tttaatgaaa taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata
    16141 taatacaacc ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga
    16201 aatgttagca taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc
    16261 agctgcagaa aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc
    16321 tagctatgcc tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat
    16381 caatactgct aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc
    16441 acctaaaaaa atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat
    16501 tacagctggc gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct
    16561 aaaaatcaac cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt
    16621 agttgccgct aacccagtag atattttgac ttactcaact tggaaattct caggtttccc
    16681 taaagaacgc gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact
    16741 tgcggaatta gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg
    16801 agattcagaa ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg
    16861 ggtaaaaaac aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg
    16921 tgacgctgct tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc
    16981 attagcacgt atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt
    17041 attcatgaat ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa
    17101 ccgtcaaggt atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat
    17161 ggctgcttct gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc
    17221 tgaataatct ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta
    17281 tcacccttat ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta
    17341 aattatgcta tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga
    17401 caagatcttc gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc
    17461 tcttagtgct gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc
    17521 cgaatacaga aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa
    17581 aattaaaaga agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc
    17641 aggaagtacc aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga
    17701 tcctaaatga acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac
    17761 aagaaataga tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga
    17821 caactaagct gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa
    17881 aagcaggtga ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg
    17941 ctgcggtcaa acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg
    18001 aattcaaaga aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat
    18061 ccatgaatgc tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga
    18121 tcccatcaag tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata
    18181 cagaacaagt acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg
    18241 acacaaaatt taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt
    18301 ggacgatcca aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag
    18361 attttactcg ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag
    18421 atacgtatat cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag
    18481 tggcccttga aacagatatc gtttccggaa agccaactac acctacacct gctggagtct
    18541 tctatgtttg gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac
    18601 cttacgagag tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg
    18661 attcagactg gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct
    18721 gcatcaacac cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc
    18781 ctgtattggt cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca
    18841 ggtgctttct ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt
    18901 ctgaaacatc atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt
    18961 cctcatttaa atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga
    19021 actctttttc caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga
    19081 atacttcagg agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg
    19141 tctcttcatc aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca
    19201 agtaactgac tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga
    19261 catttgtcgc tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac
    19321 ccggaccata tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag
    19381 cctttttaat cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca
    19441 caacaagccg caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat
    19501 aaatttcacg agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc
    19561 ttccttggat attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt
    19621 gagaatacac ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata
    19681 aacgcttctt gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg
    19741 aatccaacat gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt
    19801 cgtccaaacc tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa
    19861 tttttagctt ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga
    19921 aatgtgcgcc cttccttaaa aagaactcta aaaatttggc gtagcccaga tggttgatac
    19981 tcaccatact tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat
    20041 taggcagcca gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac
    20101 tgtaaaataa gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca
    20161 tttgtttttt tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag
    20221 aaacaacaat cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta
    20281 ttacaatatg aagggagttt tttcatgagt tattcattaa attggaatct agattctatt
    20341 ttttccggag gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa
    20401 atgaacgagt attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag
    20461 ttaaacgcta ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc
    20521 tatattactg cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc
    20581 aagctttacg caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt
    20641 gcagaaatat ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt
    20701 gcttttcgtt taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa
    20761 aacattataa atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg
    20821 attgtagcca gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct
    20881 ggacaagcct tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc
    20941 gcagcatggg aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat
    21001 ttagatggtt ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa
    21061 ccattagagt acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag
    21121 aaaaacaaac aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa
    21181 aaaatggaat ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc
    21241 tttagttttg acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa
    21301 atggctaaat ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct
    21361 gggaaacgtc ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt
    21421 atgacctaca gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct
    21481 ttccatagca gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc
    21541 gcagaaacag caagtacatt cgcagaattg atcgtagccg atgctacatt aaaagtcgca
    21601 aaaacaaaag aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg
    21661 ttcatgaata tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa
    21721 ggattagtct cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat
    21781 caaggagctt tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt
    21841 gatgacgtac cgttttataa cttcccatac acatttggct acctctttag tttgggcatc
    21901 tatgcttacg ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc
    21961 gatactgcct caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc
    22021 caaccagatt tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg
    22081 gaattgacag aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca
    22141 gaatgggcta actctttttg
//
LOCUS       DACCXM010000042        22000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCXM010000042 DACCXM010000000
VERSION     DACCXM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.044"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            39..3317
                     /locus_tag="IVY61_001325"
     CDS             39..3317
                     /locus_tag="IVY61_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP7970447.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3858..5765)
                     /locus_tag="IVY61_001326"
     CDS             complement(3858..5765)
                     /locus_tag="IVY61_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP7970448.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5895..6770
                     /locus_tag="IVY61_001327"
     CDS             5895..6770
                     /locus_tag="IVY61_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP7970449.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6850..7824
                     /locus_tag="IVY61_001328"
     CDS             6850..7824
                     /locus_tag="IVY61_001328"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7970450.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8013..9149
                     /locus_tag="IVY61_001329"
     CDS             8013..9149
                     /locus_tag="IVY61_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7970451.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9286..9630
                     /locus_tag="IVY61_001330"
     CDS             9286..9630
                     /locus_tag="IVY61_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP7970452.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9901..11187
                     /gene="efmA"
                     /locus_tag="IVY61_001331"
     CDS             9901..11187
                     /gene="efmA"
                     /locus_tag="IVY61_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP7970453.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11325..11924)
                     /locus_tag="IVY61_001332"
     CDS             complement(11325..11924)
                     /locus_tag="IVY61_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP7970454.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12328..13449
                     /gene="mnmA"
                     /locus_tag="IVY61_001333"
     CDS             12328..13449
                     /gene="mnmA"
                     /locus_tag="IVY61_001333"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP7970455.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13695..15116
                     /gene="arcD"
                     /locus_tag="IVY61_001334"
     CDS             13695..15116
                     /gene="arcD"
                     /locus_tag="IVY61_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP7970456.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15130..16248
                     /locus_tag="IVY61_001335"
     CDS             15130..16248
                     /locus_tag="IVY61_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP7970457.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16308..17600)
                     /locus_tag="IVY61_001336"
     CDS             complement(16308..17600)
                     /locus_tag="IVY61_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7970458.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17750..18310
                     /locus_tag="IVY61_001337"
     CDS             17750..18310
                     /locus_tag="IVY61_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7970459.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18439..18816
                     /locus_tag="IVY61_001338"
     CDS             18439..18816
                     /locus_tag="IVY61_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP7970460.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18749..20821
                     /locus_tag="IVY61_001339"
     CDS             18749..20821
                     /locus_tag="IVY61_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7970461.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21032..21979)
                     /locus_tag="IVY61_001340"
     CDS             complement(21032..21979)
                     /locus_tag="IVY61_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7970462.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT         7110 a         4035 c         4677 g         6178 t
ORIGIN      
        1 aagtaaacgc tttctaattt gttacgggag gaatacactt gttaaaaaaa cgtataaaat
       61 acagtgcgct aatgatgaat ctccttatgc tcagtacccc ggttttaatt tctgtcgttg
      121 aagttgctgc taacgaacaa cagtctgtga acgaagaaaa tgcacacgtg aacattgaag
      181 ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga tatggatacg gaagaaggaa
      241 atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa aaagcaatct gaagaagcaa
      301 atgaagagtg gaaaggggtt gttttcggag aaagcacaag tgctagtagg tatgccattg
      361 aaccattaga agacggcgtg cggctctctt ccacgaataa cggcggaaaa ttccagtcaa
      421 gcggatcaga tggaatgacc tattattatc aagcaatccc caaagacatc aactttagga
      481 tgcgggcgac gatcgaaata gaatcttgga cctatacgaa tgcccaagaa ggatttgctt
      541 taatggtaag agacgcagta ccaaaaaatc atttgtttgg gcaggcattt tattcaaatt
      601 catttgccat actaggcagt cggatcgaat atgtctggga ctcggataga caagaagtgg
      661 tcaacacgag cggcagcaaa tatacgatgc gacttggatt ggggacacgg gcaatcacag
      721 gaatatcatc tgaagatccg caagctgcgc cagctcctgg gagtgtctca gtggaaacaa
      781 cacctttaga gacttcagca gggttcttgg aaaaagaaac aggttcttat aatatattcg
      841 gaaacggtca tgggaatctg tttccggcca cgaactcgat tactaaactg gacgtagaga
      901 tgaaaaaaac gaatactggt ttagaggcat attattacga tcctgaaaca ggagaagaaa
      961 tggcttctga catcatgtat gactgggaaa aattatacgc ttcagatgaa tcggttattt
     1021 atgcaggatt tgctgcagca aggaatatga cgatcaaggt agaaggaatc gaagttgctt
     1081 actcagatcc tgcgacagat cctcctgggc aagaaagacc tactcgcttg attgatccga
     1141 tttacactgt gacctctagt aatcattcag gaaaccaaga tcatactatg tcctttagag
     1201 caaatgctga cggattattg acaattaaaa ataaagcaac aggagaggtg cttaagaacg
     1261 caaaaaactt ggctatcact gcgaatcggg aatttgatta tcagacaaaa ctgatggaag
     1321 gaacgaacga atttactttc agttttacgc cagataaaaa ctatccgcca gaagaatacg
     1381 tagagatgac tagttatgaa acaaaagaga ttcttcatac agtggactac cgtaaaccta
     1441 aatattcgac agtttatgta acaccagaag ggtcagatgc aggaaacgga agcagacaaa
     1501 atccattatc tctgacacaa gcattgagat acgcatatgc tgggcagaca atcgtcatgg
     1561 cgcctggaac ttattccttc gaaagaggat tgacgattcc aaaaggcgtt aacgggacaa
     1621 gagaaaaccc gattcagttg attgcagaga aaaatccaga aaacaagcgt cctgtacttg
     1681 attttaaagg aactggaaat gggatgacac tgttcagtca ctactggggt ttacgagggt
     1741 ttgacattac aaatagtgca gctatgcaaa aaggattaca gatttctgga aatcataatt
     1801 tgatcgaaga gattcatgct tatcggaatg gaaatacagg aatccaaatc agcggtagct
     1861 caaatgatcc tttcgaagca tggcccgcgc ataacttagt caagaactgt acatcttttg
     1921 aaaatgccga tccaggattt gaagatgccg atggatttgc ggccaaactg acaataggag
     1981 aaggaaatgt atttgacggt tgtatcgcgt atcataatgc agatgacggt tgggaccttt
     2041 ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat caaaaactca gttgcttatc
     2101 gaaacggctg ggtaccagga attgagggag aaggtaacgg taatggattc aaaatgggcg
     2161 gttcttcttt aacgggccct catgaattga tcaatagttt gtctttcgaa aatcttgcga
     2221 aaggaatcga ttccaatagc ggaacagata ttatcgtcaa tagcagtaca agttttaata
     2281 acggttcata taatgtggct ctttatacct cttcagcagg aaacacagac taccatgctt
     2341 ctggtatctt atctttccga acagagaatc ttgagatgag agagcagatc cgcccattga
     2401 atcaaaacga agatcaaatc taccactcgt tgaactacta ttggaatgaa cgagagaaac
     2461 aatcagaaaa tactcttgga cataccatat caaaagattg gatcgagtct ctaaatacat
     2521 gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa tggaagtatc catgttcatg
     2581 gactgatgca gattaaaccg gggaatcgtc aaacagaaaa ggaaagagtg gatggtcttc
     2641 cattatctgt aggtgctatt tttgacggag aaacctcacc gtcttcggaa tatccagagt
     2701 atgcaatcgt acaagaacca aatcagacaa aaccaaatga acaaaataat ccgattatta
     2761 tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa tatcattgaa ctgaagcctt
     2821 ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac gcctgtcttt gaagatatgc
     2881 gttatttccc agaaaagatc caaatcgttg aaacacagtc aagtgctcaa ttttctaatc
     2941 ataccgctat tttttcagca ccttcaaaag gagaatatac gctagaagtc acttatcgtt
     3001 cggaaatatt tgatggggaa aaatggtctg ctcatacaga atctgttacc cagcaaaaag
     3061 cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa tgacaaacca gaaagagaaa
     3121 atccaggaaa tgaatcacca aatggaaaac caaatggaaa accttctcat ccggatagga
     3181 cagcaaaaga aagtgaaaaa aatggcactc aaggactccc aaaaacggga gaatttaata
     3241 acccgttgct agcattagct ggaggaattt tgttaatcgg tgtagttgtt tatgtcatga
     3301 aacagcgaaa aaaataagtg gaatttctcc ttttatgatg taaaacaatt gactgaggaa
     3361 agtaaaaata gaaggaaatg agaccaatag atgtctcact tccttctatc tttttataga
     3421 tgaggttctc cttgctgctt ttgattggct gttcttatta cttgatgcag gacagctttt
     3481 tcacaatctc tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaacaaac
     3541 ggcaaaatat gaggaactat ttttccgttt ttttctctaa tactcaggtc aggacgctga
     3601 tttcacaacc tctcgataaa cggtgttcaa aagccagttt ctcggtagta agtcaaattt
     3661 atgcaaaaat gtggaaagcc attttcacaa aatttttctt actacttgaa actaagcagc
     3721 tttttcacaa cctctaatac atggtattcc agaagtaact tcttcgaaaa ataagccgaa
     3781 atgtcacaaa aatttgagaa gcgattttcc caaattcctt cttatttctt gaagctgacc
     3841 acttctggca caacctctta ccagattcgc actctttttt ctggcgctag atacatagcg
     3901 tcttgttccg tgataccaaa tgtttcatag aattcgtcaa ggttttttgg ttgcacgttt
     3961 gctcggagtt tagcaggggc atggacatcg atttgcagta aaagttgttg gtattctttt
     4021 tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga attcctcgat agaaagatct
     4081 gattccgttt ttgctgcttc caatgcacag cttagaccgc ctgcatcagc aatattttcc
     4141 gaaacggtta attttccgtt tacttttccg tcagcaaaag gcagaccatc gaattcttcg
     4201 atcattgctt gtgctttgtt ttgaaattgt gtaagatctt cttctgtcca ccagttgttc
     4261 aagttgccat attcgtcaaa taaagcgccg ttattatcaa atgcatggga aatctcatga
     4321 gcgataacag ctccgattcc accataatta gcagagcttg attgttctag actgtaaaat
     4381 ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc gaaatggatg ataataagca
     4441 ttgaccgtgt cagcactcat ttcccattct gaacgatcca ctggtttgtt ccatttgctg
     4501 aaacgatctt ttaatgtgag tttgctgaaa acaagagtat ttgaaagcaa agtaccaccc
     4561 tgatctgcag gtactgtttt gaactggtcg aataatgctg ggatcttatc ggggtagcct
     4621 acttggatac ctaagttatt taattttatg acggcttttt cttttgttgc atccccaagc
     4681 cagtcatttt cttctagacg ttttttataa acggcgatca tttttttcac catttgttct
     4741 acgtcttttt tagcagcttc gccaaaatat ttttttccgt agtaatcgcc aactacttga
     4801 tcgaattgtc cagaggcaag gtaatacgct gcttttttct gaggcattgc ttcgtctgtt
     4861 ccagacaata cgcgagaata gattccgctg acttggcgga attcttctga tagataacca
     4921 ctaagagaac gtactgtttt aatgatcatc cagcttttga ataacggaaa atgagcttct
     4981 gtcaggatgt cattcaaatg atcgaagtac actggatcag ttacgatcac tttatctgga
     5041 gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca gtttatctgt gtatttaata
     5101 aatgtatcaa aagattgcgg attatacatt ttgctgtaat ctgcgttttc ttctgcactt
     5161 tttacatgag gagcaatcaa tttatcaaat tgcactgctt gttccgtgat tgcttctgcc
     5221 tgactttttt ctttgccggc aagctctaat aactgaacag ccatatcaaa gaaaacgcct
     5281 attagttgtg caccgttggg atggtctgat gcataatacg tcttatctgg aaggaataaa
     5341 gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct tcatatctgc atctacatcc
     5401 agattaaatg gcaatggtaa tgtatccaac agccattctg gaaattgctt attcaattcg
     5461 tcgaaagact gcaatgattc aactttttcg attataggtt gaagcggttg agcatgcaac
     5521 tcatttcttc ggtcataatc gtttgccagt tgataatatg caaggaaatg tttcattttt
     5581 tctgaagaca ctttttctgg atgggcaagc agatcgtctg tttctcccat caaaagttgg
     5641 tcgatcccgt caactaaatc ttggaaaccg cctgtagctg gtttatctgc agggattttt
     5701 gctgtttttt gccattcttc attgactgct tcatagaaat cttgtttcaa tcgttctttg
     5761 ttcattcatc gatcgctcct tttttgtttc gtgtgcccat tatcttacaa aatgaaggaa
     5821 ttaacaaata gaacataaaa aattgaaaat tcgaacaatc aaactgattt gtttttttca
     5881 atccttcact tataatgaaa aaaaggaggc gatcccaact gaatcaagta gtggataaac
     5941 ctttatattt caaagtagat aaaaaaatta aaaaattagc cagcactcag cagctgcagt
     6001 caagaaaaag taaattgctt tttttagctc tggtttttga agatcaaagt tatgtgatta
     6061 tcgatcaatc aggacatccg gtagaatatt ctccagcaaa atatacttat caagagggtc
     6121 tttcttgtaa aaaatggaag ctgatcaatg aagagccaat cgagttatct agatggatca
     6181 acagaaaaga agatattccc gttttgatcg aagaaaaaag gtcaggaaag gaactcgcaa
     6241 attgctgggt gggattgcca gaagaacgtt ttttgaggta taagaaatgg gctaccccct
     6301 caggctatct ttgtggtact tacgcggcag cagttctcct tgcctattat caagactatc
     6361 gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa tacggctgat tcgttggttt
     6421 tgacgaaggc attgcgtagc cagatccaac cactaggact tcctacgatt ccttttcagg
     6481 ttagtacagg gatttctagt tttttaaaga agaatggcaa tcatgagcgt gcacgagcaa
     6541 ctcttctagg ttcatggcag cgagctacaa aacgtatacg tgaaggcaaa cctgtgatga
     6601 ttggtatttt gaaagtactt ggcagtacat atggcaatca ttgggtgaca gcgtacgctt
     6661 attttgaaac tgaaacagga gaacgttatt acaaagtaca cgacaactgg ggcgattatc
     6721 ataaagtcat tcctgccagt tggagtaacg gaacagtttc acttccttaa gaatttgctg
     6781 aaattaacag aaaatcttgg aaaaatatgt taaactatcg aagaatagag aagttttggg
     6841 gaggactaca tgagtaataa gtatcaggat gacacgttga agatttttag tttgaatggc
     6901 aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa cagaattagg taaaagcgtc
     6961 gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg aagaaagtat tcgaggggat
     7021 catgtgtata tcgttcaatc aacgaatgca cctgtcaatg attactatat ggaactactg
     7081 atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga tcaatgtcgt attgccttac
     7141 tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg aaccgatcac tgcgaaattg
     7201 atcgctaact tgatcgaaga agcaggggca actcgagtac tgactttaga tctgcacacg
     7261 gttcaagtac aaggattctt tgatattcct gtagataatt tatttacgat gccgttgttt
     7321 gctcattatt accgtcaatt aggtctgacg ggagatgaca tcgtagttgt ttcgccaaaa
     7381 aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt tgaacagcac attagccatc
     7441 gtagatcatg cagatgatgc ttcaactgag ggtggttatg tgatcggtga tgttcaagga
     7501 aagacatgta tcatggtaga tgatatcttg aacacaggag caacgcttgc acgagcagca
     7561 aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct gtgcgtcaca tggattattg
     7621 tccgatcatg cgaaaagtat tttagacaat gcaccaatca aagatatttg tatcactgat
     7681 tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa caatcgttac atgttcagaa
     7741 ttaatgggtg aagcggtcaa acgcatccat gaaaatacac cgatgagtcc gttattccgt
     7801 ctggaagaaa aagaattcga ataaacaaaa aaaagaggga aagaagttgt gacagaagtg
     7861 agcagcttca aggagctgcg acaagacttt tgtcacagct ccttttttct atgcccatga
     7921 aacgtcctct agacaaaaat gagctcttct ttggtttgtt cgctatttta tgctagaatg
     7981 tgcctagcaa taatttggaa gggatgaatg aagtgaaaac tatttatctg gatcatgcgg
     8041 caactacccc catgcatcca caagtaattg aagcgatgac ggcaatcatg caagacacgt
     8101 ttggaaatcc ttcaagcata catggatttg gtcgccacgc gcatgaaaaa ttggaaacag
     8161 caagacaaat cattgcagac agtctgcaag caaaacctca cgagatcatc tttaacagtg
     8221 ggggaacaga aggggataat acagctatct tagaaacagc gttctcgcgt caaaaagaag
     8281 gcagacatct aatcactaca gctatcgaac atcctgctgt tcttaatacc atgaaatatt
     8341 tagagacaaa ggggtttgac gttacatatc tgcctgtaga tgaaaaaggg aatctgtcga
     8401 tcgaagaggt caaaaaagcg ttacgagaag atacgatctt ggtctctgtg atgtacggga
     8461 acaatgaaat cggaaatttg atgccaatca aagaaatcgg tgaactttta gcggatcatc
     8521 cagcttattt ccatacagat gcggtacaag cgtacggaaa ccaaacgatc catccagaag
     8581 aattaggaat cgatttatta agtatctctg ctcataaaat caatgggcca aaaggcgtcg
     8641 gtttcttata taaacgagac ggagtaaata tccctgcatt gcttttaggc ggggagcagg
     8701 aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat ctggggtatg gcaaaagcag
     8761 ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa caaatataaa ggattccagc
     8821 aattgattct tgatacatta gaagcaaacg aaatccctta tcaagtaaat ggtgatttaa
     8881 cgaataaact ccctcatgtg ttgaatcttc gcttaattgg cgaagcaaat gatttgctac
     8941 tgatgaagct tgatcttaat ggggtcgcta tttctactgg ttcagcttgt acagcaggaa
     9001 atatcgagcc ttcacacgta ttagaagcaa tgtatggaga agatacaccg atactaaaag
     9061 aatccatccg tatcagcttt ggtttgggaa ataccgaaga cgacatccag tatttccttg
     9121 atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc attcttatat gaacagatgt
     9181 ctgagagaac gccttcattt tttagggaac ttaactgaaa aacctagtaa aaatcaggat
     9241 aaacttttat aataaagaga aactaatttt atcgaggtga aaatcatggc atttgaaaca
     9301 acagcaacgg tactgggcgc accaattagc tatcgtttgc atcctgatgc aaaaagatat
     9361 acattgcgcg ataatggatt tacagaaaca aacggaggca attatcaatt gatccgacca
     9421 ttagatgcca cgccgcaaag caaagaagga ttcaaattaa aaatcactgt atcaaaagat
     9481 attcaaacat tgaaaatgtc gatcactaca gcaaatggat tgaaagcagt caatattttc
     9541 aaagatccaa aacagaaaat gagccaagat aaattttatt ttctgatgga tgggatgatt
     9601 agccgtgggt tattcgaaaa agtcgaatga tctgtggata taaaaagaca actagttttg
     9661 aaacggccta ttgttagatg actaactttg taaggacgac gcaaattagt tgtctttttt
     9721 atttttaaat ttaacacatg ttgttatttt taatattttg ctgatttatt gtttctaagt
     9781 aaagaaattt aaattgaaac gattgttatt tagggaaaaa ggtatttttt gaaaaaagaa
     9841 tcccgtataa tgattgttgt gcaaacgcta tacaaaatga aaaagaaaag agggatgttt
     9901 atggaaaatg aacagtcggt cgttttaacg aattggaaga gaaattattt gttcttttta
     9961 tcgggacagt ttttgtcagg aatcactagt atggtcgtcc aatatgcgat catctggtat
    10021 ttgacaagag aaaccggttc ggcgacaatt ttgagttttg ctactttact aggaatgatt
    10081 cccatggttt tactaagccc atttgtagga ccgctagtgg atcgctggga taaaaaagct
    10141 cttttgattg tcacggatat tattgtagcg attttcgcgc ttattttagc agtagtcgga
    10201 acgatttcag aatccttccc gatttggcta gtttttgtgt ctttgtttat gcgttcggtc
    10261 gcacaaacat tccaaatgcc aacgatccag tcgattatgc cgacaatcgt tccatcttct
    10321 cacataacaa ggacgaatgg acagttagga atggtccaat cagcgaattt catcattgcg
    10381 ccagctctag gtgcggcttt gttttcagtc gttcctgtca attaccttat tttattagat
    10441 gtattggggg cagtttttgg tgtcggctta ttgatttttg tgaagatccc aaaagtttct
    10501 cctgaaatat tggaagttcc gcttactatt tttaaagatg caaaatttgg gctgcagcaa
    10561 ttgatggata ataaagggtt atggtacata acgatcaatg gtgcatttgt gatgctgttg
    10621 ttcatgccag caatcagtct gtatccattg atgacgctag attactttgg tggttccgtt
    10681 ggacaggcgg gagcagtgga agtagtctac gcagtcggaa tgctcttagg cggtgcactg
    10741 atcagtttta taggaacatg gaaagaccga atgaaaccta ttattatagc ctacatcatt
    10801 atgggcctga cgatcggtgc aagcggattg gttccaaacg atagtcaagg gttcttgtac
    10861 tttcttatct tgaatgcagg agcaggttgt gcaacgccat atttcaatac actgctgatg
    10921 gcaatgatcc agcagagtta tgaatcgaat gttttaggtc gtgtcttagg gaacttcaac
    10981 tctttgatga acctggcagg tccaattgga ttattatttg caggaccatt agctgatcgt
    11041 ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga ttttattatg cggaatcgtt
    11101 ttatttttaa cgtcagctgc ccgaaattat gataaagaat tacagaaaaa actggtaaaa
    11161 gaacaccatg agcaaaaaga cgaatagtat ggaatggagt tgtgacagaa gtcttgttac
    11221 aactccattt ctgatcctaa tgttcctgtg gcaataagcc tactcaaatg aaaaatatga
    11281 gtaattatct ttatttgttt aatacttgat atagggcagt tttttcacga cctatcgtac
    11341 ttcctctttg cttcatagat ttctttgatt tgttgctcag ataacggagt gccaaatggt
    11401 agtttttcct ttaagagttc tctttccggt gcgtctacgc ctacattgac atcgttctcg
    11461 ataggtacgc tgtaatgatg gatatgtcca tgaagattgc ggatagtgtc gttaggcccg
    11521 agcaacatcg gataatgagt caagtaatat tgttgatggt cgaatttaac gatggcacct
    11581 acatcataaa aataaaattt gggacttccg tcagtaagcc taggatcatt tttttctaaa
    11641 taacggaaaa aagcgcgact gtcatggttc cctttgatca agtggattgt tccttgcagt
    11701 tgagtgacca gttccaaaat ttctgcattt gctttttcat attgcggatg catagcaagg
    11761 tcgcctagat gataaacatg gtcggtttct tttattactg cattccagct agtgatcaac
    11821 tgttggttca tttcatccac atttgcaaag agccgcggag caaagtcgtt ttttcctaat
    11881 aagcgttcat ggaaaaagtg catatcactg ataaagtaat tcatgtatct tttgcctccc
    11941 ttttttctta gagtaaacaa aatcacttat ctgagcaact cataacatct aaaaagaaaa
    12001 ggttgtaaat gagacgtgca gctttaagcg ataagaagca attttgaaaa atagtttttc
    12061 acaatttata aaattattcc ttattgccga aaggctggcc ctttttatga gtatgccaat
    12121 tttttatatc gaatgctcaa aagatgagtg attttttggt tgactatcat gtttgtgtac
    12181 tgaataggtc cagtagaaca gtatttttac gacttgataa ttaagggatg aaattttcag
    12241 aaatatgacg tataatgtaa aatactgaaa agttgcgacc ttcaactcgt agatgtttca
    12301 gaatctaatt gaaatgatgg tgatatcatg acagacaaca gcaaaacacg cgttgtcgta
    12361 gggatgagcg gcggtgtcga ctcatctgta acagcacttt tgttaaagga acaaggatac
    12421 gacgtgatcg gaatattcat gaaaaattgg gatgacacgg acgaaaacgg tgtatgcacg
    12481 gccacggaag attataagga tgtagcgaaa gtagctgctc aaatcgggat tccttattac
    12541 tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg aatatttcct agccgaatac
    12601 cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca aagaaatcaa attcaaagca
    12661 ttcttggatt atgcaatgga tcttggcgcc gattatgtag ctactggaca ttatgcgcaa
    12721 gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg gagtagacaa taataaagat
    12781 caaacttact tccttagcca attatcacaa gaacagcttt caaaaacgat gttccctcta
    12841 ggcggcatgg aaaaatcaga agtacgggca attgctgaac gcgcaggcct tgcgactgct
    12901 aagaaaaaag attcaacggg aatctgcttt attggtgaaa agaacttcaa acaatttttg
    12961 agcaactatc tgccagcaaa aaaaggaaat atggtcactc ttgatggtga agtcaaagga
    13021 caacatgctg gcttgatgta ttatacgatt ggtcaacgtc aaggactagg gatcggtggc
    13081 ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag atctagcgac aaacacatta
    13141 tatgtggggc aagggttcca tcatcctgca ttgtatgcca caagcttgga tgcaagtgaa
    13201 atccacttta cgacgaatga accaatgcca aaagaattca agtgtacagc gaaattccgt
    13261 tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg atgataatcg tgcagaagtc
    13321 gtattcgacg aacctgtacg tgcaatcact cctggacaag cagttgtatt ttatgatgga
    13381 atggaatgtc tgggtggtgg tttgatcgac cacgcatatc aagaaacaaa agttttacaa
    13441 tacgtttaag cgatattaaa tgaatcaaaa cagctatggc taacaaaaga aacacctttt
    13501 cttttggata gcatagctgt ttttttgcaa taaaattaat ttttattcat tttatacttg
    13561 aaatacttgt gtaaaaagtt cataatatcc aataatatga ataaagttgc tttttgttaa
    13621 gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt gatttgttca attttataag
    13681 tgagggtgat ggaaatggaa aagaaatcga cgggtattag caaatgggga ctggttgctc
    13741 tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat cacaagtgat ctggctagtt
    13801 ctgcagctcc agggccagcg atcctttcat ggataatcgt tgggattggt attttagctt
    13861 tggtattatc gctgaatcat ttaggagaaa agcgtccaga tctagatggt ggtattttcg
    13921 gttacgcgga agcttcattt ggtaaattag gtggcttcat cagtggttgg ggttactggt
    13981 tgtctgcgtg gctgggaaat gttgcttttg caacgatgct gatgagcgcc atgggagaat
    14041 ttttcccgat attcaaaggt ggacaaaatg ttccttctat tttatttgca agtattttta
    14101 tttggggtct gactttatta gtaaacaatg gagttgaagg agcgagcgtc atcaatacaa
    14161 tcgtgacgat ctgtaaatta gtgccattat tcctattttt aatatttgta tttatcgcat
    14221 ttaaagtgaa tatattcact gctgattttt ggggtaacgt atctgataat ctagtgaatg
    14281 gaacagggca gcaaggaacg atctggttgc agattaaagg ttgtttgatg gttatgatgt
    14341 gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa ccgcgcgaag aaacgttcag
    14401 aagcccagca agcatcgatc attggtctgc tttgtttatt gtttatctat atccttgctt
    14461 cgatcttgcc atacggcgtc ttatctcaag aagagttagc aactatttca caacctgcta
    14521 tggcaaatat tttaaaagga atcgtgggaa attggggagc agcgctgatc aatatcggct
    14581 tgattatttc gatcattggt tcttggttgt cttggacgat gctcccagca gaaacaacga
    14641 tgttgatggc tcgagacggt ctgttgccga aaggttgggg aaaaacgaat aaaaaaaatg
    14701 ccccaactta ttcgttagtt gttactgcaa tcttgactaa tctattctta ttgactttct
    14761 tagttactga ttatgcctat caatttgcct attctttatg tacagcggca attttgattt
    14821 gttatctact tgtcgggatc tatcaagtcc agttttcttt aaaagaagga atcaaaaaac
    14881 aagtagtgat cggtgggatt gctgtagttt ttgaattgat gggcatcatt ttagcaggat
    14941 tttcttatgt ccttttatgt agtattgcct atcttcctgg tttttgcttc tactggatcg
    15001 cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa agtaatgatt gggctgattt
    15061 gtgtggccgc actgctttca atcgggttgc ttgctgtagg ctggatcaag atataagggg
    15121 gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat ggctaaataa atgggaaaag
    15181 aaagcggtat atgatattgc acaaagctcg atcgaagcat tgacattaga cgaactcgtt
    15241 ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac gcaaaaacga accattggac
    15301 tatggctgga tcgaaggatc agatgttttc aaacaagaag tcgcttctct ttatcaaacc
    15361 gtcgatccgg aaaacatctt acaaacgaat ggagcgactg gtgctaatct tttagctcta
    15421 tacgcgttag tggaagcagg agaccatgtt gtttcgatgc ttcctactta tcagcaatta
    15481 tatgacatcc ccaaatcttt aggtgccgct gttgactttg ttcatttgaa ggaagaagaa
    15541 gattggcagt ttgatttaga acagctagaa gccttagtca aacctgaaac aaaaatcatc
    15601 tgtttgaata gtgcaaacaa tcctacaggc acattgctag atcggaaaac tttagaaaaa
    15661 atcgcagaaa ttgctcgtac agtagatgca tatgtattga tagatgaagt atatgcgcct
    15721 ttaacggata aaggtgaatt cttgtcgatc gtagatgtct atgaaaaagg tatagcaacc
    15781 aattccctat ccaaaaccta ctccattcca ggtattcgta ttggctggac agctactgga
    15841 cctgagttgg cagaagtttt tcgaaagtat cgagactaca cgatgatttg cggcggagtc
    15901 ctgtcagatg atcttgctgt acatgcattg aagaataaag aaaaaatatt ggcgcgaaac
    15961 aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat gggtagccaa tgaaccgaaa
    16021 gttgaacttg tcgctcccaa ttacgtctct acttctttca ttaaattagc catcgaagaa
    16081 gatgaccaga cattttgtat caatttatta gaagaaacag gagtactatt agtaccagga
    16141 agtgcctttg atttaccgaa gcatgcccga ctaggttact gttgtaaaaa agaaaccttg
    16201 gaaaagggat tattcctctt atctgagttc ttgaaaaaac aggcctaaac aaaaacactg
    16261 caaggacagt ctgtgacaac tgttcaagca gtgtttttca tttacgctta ttgtctatga
    16321 gaaggagagt gccctgaagc tatggagact cctgaagctt tttcttctgc agtttcttgc
    16381 tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt tgaccaaaac aagaagagaa
    16441 gtcgaaagaa agacgccgcg ataaccaaat gcactggaaa cactggagcc gatcataggc
    16501 cctactacgt taccagttgc ttggaaagat tggttataac tgaagatccg tccagcagct
    16561 tgatgaggcg aatattttgt gatcaatgct tggacagcag ggagcagaca agcatcagag
    16621 ataccaatca aaaaacgtaa ggcagctaat tgccaaacat tcgtgacgaa tgccattggg
    16681 atataaacca gaatcgcaaa acccaaacca atcgcaagta ttctttcact cccaatcttg
    16741 tcacctaagc gaccaaatct tggagcggct atcaaagtgg caatcccagg tatcgaagca
    16801 atgattccgc tgatcaacgt aacattccca tgtccatgca gcaattggcg aatgtacaga
    16861 ctgatgattg gactaatgga attgttggat gcttggatga tcatcgttgt gacgaacata
    16921 ccgatgacga cgtgtggata tttcaagtct ttgaaaatct gtttggcaga aaccatgttt
    16981 tcttttttca ctgggacaaa tttttcatgg acgaaaaaca gactcaacaa gaagacgaaa
    17041 aataagatga tccctgtaat gaaaaaggta gggcgatagc caaacgctga agcagagatc
    17101 ccgccaagaa gcggtccaag taatgttcct gtaacagaac cagttgctaa ggttccaaga
    17161 acttgaccac ttttttcttt tggcgtccca gttgcgacaa gggcagtggc attactgata
    17221 taaccagaaa aaatcccttg aagcagacgc aatgcgacta attgatacac actagtcacg
    17281 caacccatca aagaaatgac gattgccatt cctaatgaag cacgcaacag catcaatttt
    17341 cttccttttt ggtcggctaa ttttccccac caaggagaga taagcgtagt caccaagaaa
    17401 gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg acgtactata attacctaac
    17461 gtatctatgt aaagtgacat aaaaggcatg actaaactaa atccgatacc agccataaat
    17521 gttccaaacc ataagacaaa caagttttgt tcccaagttt ctcttttcct aaataatttt
    17581 tcctttattc ttgcagccaa ttagactcct tctttctgaa aattttcatc tacagctctt
    17641 tattataaac tacaatatgt agtttatcaa tgaaagtctc ttttttctct ttctttttcg
    17701 tggtaaactg aaagagtgaa ggatttattt tatgagagga aacatggaaa tgaaaaaaag
    17761 aaatctgtct caagagaaaa tcatcgactg ctttcgagaa ctagcagaag agatggatgt
    17821 tcagcaaata acctttcagc atttagccaa acgcttggat atcaaggctc cctcgctgta
    17881 taaccatttc aaaaatattc gtgatgtcaa aacagcgcta acagcgaaac ttctgcagga
    17941 acttaatgac cagctgcgac gggctcttgt gggaaaaagt ggtcaggaag cactgcagat
    18001 ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa gcagtgtatg agttactgat
    18061 cagtgtaccg cataccaatg aagctgtttt attagatgga atccatgaaa cgaatcagat
    18121 cattttgcag atatttgagg cttttcaatt aacgaaagaa gaaaaagttc atcgaagcag
    18181 ggaacttcgt agtatgatcc acggttatct ttccttacgt tttttaggat actttacaaa
    18241 agaaccaaca atcgatccag aagacagcta tttttggatg atcaataact ttatcgctac
    18301 tttgccttaa acaatcagat tcatttgtaa caattttaag atgttcatta aatgaccctt
    18361 ttataagaga aaattcttgc aactttcttg aaaagtgact aagatgagtc tagaaaagct
    18421 gaaagaagga gcacgagtat gtatcaagta attaccatgt tcggggacaa tgagccttgg
    18481 tggttttttg aagactggga agaagatatt gaagaagaag aaacattcga gtctttcgaa
    18541 gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc atcaaaatta tgaatatatc
    18601 aatgcaaaaa gtaactttct cagcgccttt tggaatgacg gagatgagcg ctggtgtgaa
    18661 gagtgtgacg atgatttaca gcaatacaag ggacttgcgt tgttgaaaaa tcatcagccc
    18721 atcacagtag aaagcagaaa ggaattttat gaaacaacta attctagcgg aaaaaccaag
    18781 cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa aagcacaaaa attattatga
    18841 aggacccaag gtgatcgtta cttgggccct tggtcattta ttaggattga aaatgccgga
    18901 agatctgaat aaggaatggc agagctggca aatggacacg ttgccaatga tccctaaaaa
    18961 actaggaatc aaaccacttc ctaaaacagg acatcaatta aaagcaatca agcagctggc
    19021 tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat gccggaagag aaggggaact
    19081 tgttgctcgc tggatcttag agtatgtgca tttcaataaa ccagtgaagc ggttatggat
    19141 ctcttctcaa acagataaag caatcaaaac tggattcaag caactaagac ctgcgaaaga
    19201 atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa gcggactggc tggtaggatt
    19261 gaatgtgacc agagccttaa ctgtgaagta cgcggataat ttatcagcag gccgtgtgca
    19321 gacaccgaca ctagcattag tgagagacca agaaaagaaa atcgagcagt tccgtccaca
    19381 aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa gccaaaatgc ttcaaaaaaa
    19441 tcaatttgct ttaaaaacac aagaagaagc acaaaaactt gttcaattga tcagtacaag
    19501 caaaggaact gtcacagaag tccaagaaaa attaaaaacg gaatctgcgc cattgcctta
    19561 tgatttaaca gagatccaaa gagaagccaa tcaacgctat ggctattctg caaagaaaac
    19621 attaagtctt gtacaaagtt tgtatgagac ccataagatc gttacttatc cgcgtacaga
    19681 cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa gaacgattgc aagcagtcag
    19741 cgactttgct cctgaggtca agcagtattt gaaaaacgga gccatcgtcc aacagacaaa
    19801 agtattccaa gacagtaaag tgaccgatca ccatgcactt ttgccaactg aaaacagagc
    19861 acgttatgaa aaattaagta atgaagagcg aaaaatctat caaatgattg tctctcgttt
    19921 tttaggactg tttgctcaac cccataaagt cagccaaaca aaagttacgg ttgaatttgg
    19981 taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa gcaggatgga aaggcgaaac
    20041 tgaaactgag acggaaacag tcaaatggga aaaaggaatg agaatcaatc ccgatttcgc
    20101 catcaaaaaa gaactatctg caccgcctaa accattgtca gaagcaagct tgcttggtca
    20161 aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca gagattatcg agaaactaat
    20221 caaatctgaa ctgatggaac gtacccctca aggactgcaa gtatctccta aaggtaagca
    20281 attattagta ttggtaaatc cctctttagt aacaccagat ttgacagaaa gctgggaaaa
    20341 agaccttgaa gctattgctg caggaaagaa acaagcagga accttcttga atggaatcga
    20401 aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc aaacaagaat atcaagattt
    20461 ctcgattacc caaaagaaat gcccagaatg tggggccaat ctgcgcgaga aaaacacacg
    20521 tgacggcaaa atctatgtct gcaccaattc agattgcaac taccgcagaa gaaaagatcc
    20581 aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa atggaaatca tcgaaggtaa
    20641 aaacggtgct tatttccgtt gtaaatacga tggaacgaca gaaaaattgc ttgataaaaa
    20701 agagcagaag aagaaaatga ccaaacatga agaacgacgc ttgctgaaaa aatatgcaca
    20761 agcagaagaa ccagaagaaa gcccattagc agcagcacta aaagcagcaa tgaagaagta
    20821 ataagatatg aaagaggttg tgatagaagt ggtcagcttc aagaaataag aaggaattta
    20881 cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat ttccgaagaa gctacttctg
    20941 gaacaccatt tattcggaaa aaaggactgt gacaagactt ttgtcacagt cctttttcat
    21001 ttaaaaaaat gtttacttca tttgacgtat attattggtg aggcttcaat tcatttggct
    21061 tcactttttc tgtttctctt atgatataaa caggacgctg tttcgtttct aagaagattt
    21121 tcccaatata tttcccgatg attccaagac ataacaattg gattccacca atgaacagga
    21181 agatcgagac catggaaggc catcctgatg taggatcacc aaacaacaat gcccgcaaaa
    21241 taatgaacaa aattgcaata ccagaaccga cacaagacaa agcgccgata aatgaagcaa
    21301 tcgttaacgg tgcatccgag aagttcacga taccatcaat cgagtagttg aataatttcc
    21361 agaaggacca agatgtttcg ccagccacac gttcgcggtt ttcataagaa atatattctg
    21421 ttttaaatcc gacccagcta aataatcctt ttgaaaatcg gttgtattct gttaattcta
    21481 ggacggcatc caccatttga cgagtcatca agcggaaatc acgagcgcca tcgaccattt
    21541 ctgtatcgct gattttattg atcaactggt agaatttttt cgcaaagaaa ctgcgtatcg
    21601 caggctcgcc ttcacgagtc gaacgccggg tgccgacaca atcgatatca gaagtagtga
    21661 tccggttgat catctcagga agcagttcgg gcggatcttg cagatcaaca tccatcacgg
    21721 tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag agccgcttct ttcccaaaat
    21781 ttctggaaaa tgagacaaag cggacataag gataagctga ggctagcttc ttgattgctg
    21841 ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata ttcgaactca tgggcacctt
    21901 tgaatttttc aacctcatca aaaaaaagag gaacagtttt ttcttcatta aaacatggga
    21961 caacaattga tactttcatc gtttctatcc tttcaatcgt
//
LOCUS       DACCXM010000043        21874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCXM010000043 DACCXM010000000
VERSION     DACCXM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.045"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            23..172
                     /locus_tag="IVY61_001341"
     CDS             23..172
                     /locus_tag="IVY61_001341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970463.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            665..1993
                     /locus_tag="IVY61_001342"
     CDS             665..1993
                     /locus_tag="IVY61_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7970464.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2195..3028
                     /locus_tag="IVY61_001343"
     CDS             2195..3028
                     /locus_tag="IVY61_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP7970465.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3044..3781
                     /locus_tag="IVY61_001344"
     CDS             3044..3781
                     /locus_tag="IVY61_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP7970466.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3936..4904)
                     /locus_tag="IVY61_001345"
     CDS             complement(3936..4904)
                     /locus_tag="IVY61_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP7970467.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5136..5969
                     /locus_tag="IVY61_001346"
     CDS             5136..5969
                     /locus_tag="IVY61_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP7970468.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            6003..6842
                     /locus_tag="IVY61_001347"
     CDS             6003..6842
                     /locus_tag="IVY61_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7970469.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6868..7428
                     /locus_tag="IVY61_001348"
     CDS             6868..7428
                     /locus_tag="IVY61_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7970470.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7627..9348
                     /locus_tag="IVY61_001349"
     CDS             7627..9348
                     /locus_tag="IVY61_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP7970471.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9513..10655
                     /locus_tag="IVY61_001350"
     CDS             9513..10655
                     /locus_tag="IVY61_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7970472.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10671..11882
                     /gene="thiI"
                     /locus_tag="IVY61_001351"
     CDS             10671..11882
                     /gene="thiI"
                     /locus_tag="IVY61_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP7970473.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12455..13102
                     /locus_tag="IVY61_001352"
     CDS             12455..13102
                     /locus_tag="IVY61_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7970474.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13495..16140
                     /locus_tag="IVY61_001353"
     CDS             13495..16140
                     /locus_tag="IVY61_001353"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP7970475.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16450..17763
                     /locus_tag="IVY61_001354"
     CDS             16450..17763
                     /locus_tag="IVY61_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP7970476.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17753..18406
                     /locus_tag="IVY61_001355"
     CDS             17753..18406
                     /locus_tag="IVY61_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7970477.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18461..19153
                     /locus_tag="IVY61_001356"
     CDS             18461..19153
                     /locus_tag="IVY61_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7970478.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19303..19503
                     /locus_tag="IVY61_001357"
     CDS             19303..19503
                     /locus_tag="IVY61_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7970479.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19757..21436)
                     /locus_tag="IVY61_001358"
     CDS             complement(19757..21436)
                     /locus_tag="IVY61_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7970480.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21438..21650)
                     /locus_tag="IVY61_001359"
     CDS             complement(21438..21650)
                     /locus_tag="IVY61_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP7970481.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
BASE COUNT         7229 a         3777 c         4598 g         6270 t
ORIGIN      
        1 atagctcagt tggtagtagc gcatgactgt taatcatgat gtcgtaggtt cgagtcctac
       61 tgccggagta aataaagctt atgcaaagaa aaagaaatcc aatcacttgg gtttcttttt
      121 cttttattat tatgatgaat caagctttct gtttttgtca cgtatgttgt aatctttacg
      181 aaagagtaga gttggctact cataatcaac catacttccc atcatcgcta aatacgaaat
      241 aaatcagcat tcctacacca atcaaagcac aagctgcacc aagaaaaggg atgacattcc
      301 caaagttatc tgtattcata agtatgccac cacccacaaa caaaaagccg ccagcaatca
      361 ggtttttcat tttattcatc tcctttctta cgttatagta tttccttctt tcaatgagta
      421 ttttgaaagc tatcttattg tatgatgttc ttctaggcct cttctttatt atacaggcca
      481 ttttcgttat ctcatagaaa tattcctttt tatctgaaaa ctattgtata ccgtatgaca
      541 tttttcttca tgaagttgta agtatattct cattcaaatg aaaatttcat catagaagag
      601 attgtatttc taaattctta agagtaaaat acgaagtata acttggattt ggaaggatga
      661 ttggatgaaa gctattacgc ctgatatgac aacaaaattt aacaaacagt ttcaagcaaa
      721 tatcaaacag caggcattgc agcgggcagt tgtgaagaac ggaattactg cttctgctga
      781 aaatgtttca gcacatgtgg agaatacacc ggtattttct atagatttgg ctacggggaa
      841 agtggctaat caaatgcaat cggggcgttg ctggatgttt gctgcactga acacattccg
      901 tcataagatg ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa attacacttt
      961 tttctgggat aaatatgaaa aggcaaatta tttctatgaa aacatcattg caacaggaaa
     1021 agaggcaatc gatagccgga aagtagcttt cttactcgct actcctcaac aagatggcgg
     1081 ccaatgggat atgatcgttt cattattcca aaaatatggc gttgtgccta aatcggtgat
     1141 gccggaaagt gcgaatagtt caaattctcg agatttgaat aactacttga acaaattact
     1201 gcgtaaagat gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat tagacgctat
     1261 tgaagaaaag aaagaagaaa tgctagaaag catttataac ttcttagcta tctcattagg
     1321 aacacctcca aaagaattcg attttgagta ccgcgatgaa gaaaagaact atcatcttga
     1381 ccgaggactg acaccacaaa ttttttatga taaatacatc ggtgtaaaat tagatgatta
     1441 tgttagcgtg atcaatgcac caacaaaaga caaaccattc aatcgttctt atacagtaga
     1501 gatgctagga aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg atatggaaac
     1561 tttcaaaaaa ttagcgattg ctcagcttga agaaggagaa tctgtttggt ttggttgtga
     1621 tgtaggtcaa tcttctacta gaactagtgg catcatggcc ttagatgctt attcaatgga
     1681 tgatctattt gatacggatt ttacaatgac taaagctgaa cgtttagatt ttggagaaag
     1741 tctgatgacc catgcgatgg tattgaccgg tgttgactta gtagacgggc aatcaacaaa
     1801 atggaaagtc gaaaatagct ggggcgaaaa agttggaaag aatggcttct ttgttatgag
     1861 tgatgcctgg atggacgaat atacgtacca aatcgttgta agaaaagatt tattgacgaa
     1921 agagcaatta aatgcgttca atgaagaacc aattgttcta tctccgtggg atccaatggg
     1981 tgctttagca tagacgagta tttcaatgaa gagtatgtag taaagataaa aagagctggc
     2041 ttaagccagc tctttttatc tttaaagtcc attccctgtt ttttatggtc cctgtgctat
     2101 aattctaaat aaaggataag gattggaaat aatcctttta tcattaaaaa tatgatgacg
     2161 aagtagaata aaattgggag taggggggaa agtcatggat atccaaactg ttttatctga
     2221 tctgaaatta aatggtaaag tgattccagt tgttggtggc gatgttaatc agacctatcg
     2281 tataaaaacc gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa aaaaaggatt
     2341 ttttgaagca gaagtagacg gattaaaaga actgtcagca ttcgtacgtg taccggatac
     2401 gtatatgcta ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga tcgagccggg
     2461 aaaaggggat caacgagatc ttgctgcagc tttagctaat ctccatcagc aaacagcgcc
     2521 acaatttggt tttcgtaaag ataactattt gggtacactt gttcagaaaa attcttttga
     2581 agaagattgg tggacttttt tctttaaaga tcggcttgaa tcccagattt ctttggcaga
     2641 agaaacgaat cgatggaatg tacagcgtca agaaaagtat ctgcgattta aagaacgtgt
     2701 attgaaatcg gttgaaccaa aaaagatcac tccacgcctc ttgcacggag acctgtggag
     2761 tggtaatgtg ttctttgacc agcaagggca tccagttttt gtcgacccag ctgtttctta
     2821 tgggaatcgg gaacaagata ttgcaatgag ccagttattc ggtggtttcc gaccggaatt
     2881 tttagatgct tatcaaacta tatttccatt agaaaaggga tggaaagacc gactgccgat
     2941 ttaccagtta tattatcttt tggcacattt gaatatgttt ggagaaagtt acggttccca
     3001 agtagatcaa ttactagaaa atttttaaga ggagggcgtg tttttggcaa aaaaatatac
     3061 caaaaagcat gaagtttcat attatgagtg tgacatcaac caaacgatga catttccttc
     3121 gatgttagga gtagttatca aaacgtctga agatcaaagc gaccagttgg gaagaggcag
     3181 tgaattcgtc aatagctttg gacttacttg ggtcatcacg aattattcca tggagattaa
     3241 tcgtctccct agagtcggtg aagtaatctc agtaacgact caagccatgg agtacaataa
     3301 gtttttctgt tatcggaact tctggatcca tgatgaagat gggaaagaat tagtcaaaat
     3361 agaatctgtt ttcgtattga tggattttgt gaatcgaaaa atgagcagtg ttaatgaaga
     3421 gatcattgct ccttttgaga gtgagaagat caagaaaatc aagcgtcagg aaaaaattga
     3481 aaagattgat tcgggcttca tgttgccata tcgtgttcgt ttttatgata tcgatagtaa
     3541 tcagcatgtt aataatgcga tgtattttaa ttggatcatt gacgtattag gatatgactt
     3601 tttgactacg catgttccag aaaaagtaat tattcgattt gataaagaag tagagtacgg
     3661 gaatgagatc gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca aaacaaaaca
     3721 tgaaatccga atgcaagaac agctgtattg tgaagcaaat attatatgga aaaataaata
     3781 aaaaagaggt tgtgacagaa gggggcaact tcaagaaata agaagaaatt tacgaaaatt
     3841 gtttctcaaa tttttgtaaa ttttcgactt agttttcgaa gaagctattt ttgtcacagc
     3901 ctctcttttt aagtttagct attagataat gatgtttagt tttggataaa cgaaatcaat
     3961 tcttcacccg acaaaccata gtttacagca atcaatagtt ttaaacgtgc tttttgacta
     4021 ttaatactgt tacagaaaat aatccccatt ttttctagtt cttttccccc gccttcataa
     4081 tcataaacag gctctgcgat tccattgaaa caacgagaaa caagtactac agggattttt
     4141 tggacaagaa gcttttccaa agcagctaag gcttgtggag ggagattccc cgcacctaaa
     4201 gcttcgatta ccaaaccgtc tactttcgta tgggcgatgg cttccaataa gtccccttgc
     4261 atcccagcat atgctttaac gatcgggata gtcccatcta cagcggaaat gtccagtcgc
     4321 tttgtttcta aaagttcctg caagaataaa attcgattct tagttactaa gccgatggga
     4381 cccaatgtag gtgttcggaa agtagcaaca tttgtggtat gggtctttgt cacgtagcgt
     4441 gcagaatgga tctcatcgtt catgaccacc aaaaccccct tatctagcgc ttcttcacaa
     4501 cttgcgacac gaatcgcact ttcaaaatta taaaggccgt cacttccaag ttcattactt
     4561 gaacgcattg ccccagtcaa aacaattggt aatttgttgc caatcgttgt atcaagaaaa
     4621 aaagcagttt cttctaatgt atctgtaccg tgtgtgatca ccactccgga tatacctgat
     4681 gcataagcgg acaagatacg ttcttttaat accaacatgt gagaaggcat gatgtgtggt
     4741 gagggaagat tgaagatatc ttcaactaat aattctacat gcttggggat tgtgatttct
     4801 gcatctaata atgggtttgc cacatcagga ctaacaccgc ccgtgagcat gttttctttc
     4861 atggcgatcg ttccgccggt gtgtagtaca agtatttttt tcattttaca gacttcctta
     4921 cgaataattt tgcatagccg ttaaatctta ttgtatacta tataaaagag ttttgctatc
     4981 agtgaagaat aacgaatagc ctaacttatc ataaaaaagc tgaccttatt aaaatggaaa
     5041 agactcaacc ttgacctcct aatacaatgg agtatctggt tgagtttctt ttacaagaaa
     5101 gcagccaagg aaatcaatca cgaaggtggc cggaaatgat cagaatcgga ttaacatcgt
     5161 ttaaagaaca tgacaaatta actggtaaaa agaataacac tctttatgaa tatgcagggt
     5221 ttctaccctt agtggaatta gatactgcct attatggaat tccccgaaag tcctcagttg
     5281 agaattggat aagtgaagta cctgaatcat ttcgctttgt agtcaaagtc tatcaaggaa
     5341 tcagcggtca aggagactgg aagccttatt atcagaccga agaagagatg attgcgcatt
     5401 ttttagatgt tatgtcgcca atggtagatg ctggaaagat gttttgcttt ttagtccaat
     5461 tcccctcatc attcaaatgt acgaaagaaa atgtccagta cttgcgcaaa cttagaaagt
     5521 ggtttgaggg ttatcctgtt tctatagaat tgcgggatta ttcttggtac agcgatgaat
     5581 ttcttgaacg tacaagaaac tttatgtcta gagccgcttt ttctttagcc atcatcgatg
     5641 agccacagtt gctgaataca acgattcctt ttgatccatt tgtcaccaat ccgaactttg
     5701 ctctttttcg attccatggt aggaatgcgc aaggatggca agcaaatgat aaagagtggc
     5761 gtaaaaaaag aacgttatat cgttattcag aaaaagaact gcaagaacta gcacatgaag
     5821 tcaaagtagt tgattctaag acaaaagagg taggtgtgat ttttaataat aattctggtg
     5881 gtgatgcggc tgaaaatgca ttagcactgc aaaaaatatt aaatatcaag ccagatggac
     5941 tcaatccaac ccaaatggat ctgttttaag ggcttatttt attcaagaaa ggggcgaagt
     6001 ctatgatcaa agctattttt tttgatattg acggaacatt ggtcaccaat cgttcaaaag
     6061 cgttggaatc aacaaaacaa gcaattgaat atgctcggaa aaacggcatt ttatgcggtg
     6121 tagcaacggg gcgcagccca gtaaagatca aagaaatcat tgatgaattg gaattagata
     6181 tgtatgtcgt gtataatggt caactggttt ttactgcaga tcgcacgatt atcgaccacc
     6241 cttttgaaca aaaagtattg gaacatattg ttgaatttgc agatgagaac catcgacaaa
     6301 tcgtttttgg tgcgagaaat cggttagatg gatcaacgac gatgctatta ggacaatcga
     6361 tttttatcaa gcgactagtc agctttttac cgcgtaaatt cccggtacgt ctgatgaaaa
     6421 aaatcctcca ggtttttagc cctcaccgac aaaaggatcg ttatgaaacg ttagagatat
     6481 taaaagaacc aatttatcaa tgtatattgc tcagtccgga gtcagaacag aaaaagttag
     6541 aacatcgctt tcccgagtgt acgtttcagc gttcaaatac gtatactgta gatatcatcc
     6601 caaaaggtgg ttcaaaatta ctaggcatcc aagctttcgc aaatgctgtc gaaattgaaa
     6661 tggaagaaat tatggcattc ggcgatcatt acaatgatat cgagatgctg aaaggtgtag
     6721 gaattggagt ggccatgggg aatgctcaga tcgaagtaaa acatgctgca gactttgtga
     6781 ctcaatcaaa tgaagaagat gggatatacg aaggactgaa acacttcggt ctgatttatt
     6841 agtcaatgaa agaaggaaaa aatcagtatg gaagcagaga caccttttca aaaagtagaa
     6901 acattccaca aaacttttga tccaagaaga ccagagatac ccacggcttt tacagaaaat
     6961 gaagctcata cacgtgctgc gttcaaaata gaagaacttg ttgaatttgt tgcagctact
     7021 tcacctaata aagaacaact gcatgaagct gttatgctgc tgcacgaagc attagataaa
     7081 gcagaagaaa aaatacttgg taaaacacag tggggaagtg atccgctggt tgaacaagta
     7141 gatgcactga cagatatcct ttactttact tatggttctt tttcattact aggaatagtg
     7201 cctgatcgaa tattcgaaat cgtccatcag gcaaatatgg ggaaattatt tccagatgga
     7261 aaacctcatt atgaccctgt aacaaataaa gtgttgaaac ctgaaaactg gcagatggaa
     7321 tatgctccag aacctaaaat cgcggcagaa atccaacgtc agattcaaga agcgattgct
     7381 aagaagaaat ccgctgatat aggcatgtgc acaaatgaaa aagagtgatc agagtttcac
     7441 tatttttgtt atctttatac gatggaaaaa caaaagattt tcgtttaagt tagtgaatgc
     7501 atgaaaatag tatgctataa tctagaatag atgtcttttg cacgagagac gtattatttg
     7561 gatttatgcc aagcagaacg ccatctgttg cataaaagat gcatactgaa aacggaggaa
     7621 ccataaatgg gaaataatgt tatcatcgga atcgtcattg caatcattat agtcgccatt
     7681 gtcgtctttt tggttagtta cttgatgaag aaaaaaaacc aagatcgatt ggatgcctta
     7741 gaaaaacgca aggaagaatt atttgacttg ccagtgattg aagaagtcga tgatgttaaa
     7801 aaaatgcatc tggttgggca aagccaaaat acctttcgtg aatggagtca aaaatggaat
     7861 gacatatcta cttcctcgtt cgctgaacta gaaagtcaaa ttttcgaagt ggaaaacctc
     7921 aatgaaagtt ttcggttctt aaaaggaaaa aaagcagttg ttgaagcaga agctacaatg
     7981 gaccaaatgg agaaacaagt gacggaaatc cgtgaaggtc taaaggaatt gcgggaaacg
     8041 gaagaacgca actcactagc tgttcagcag gcacttgacg tctatgaaga actgaaagaa
     8101 aaagtcagag aagacgagca tgaatacgga ccagctcttt ttgaattgca aaaacagatc
     8161 aaagtgatcg aaaaagaatt tacgcagttt gtgactttaa acacttctgg agaccctgtt
     8221 gaagcccgcg aggttttgga taaagcagaa aaacatacgt atgaagtgga agctctgatg
     8281 aaaaaaattc caccgctgta tgaggagctc cataccactt tccctgaaca acttgaagaa
     8341 atcagtgata cttacgagaa attgatggaa gaacaatatg tattcccaga agaagatctt
     8401 gctgaagata tcgcaaaagt aagtcgaaga attgaaaatt cattagccaa tcttgaaaaa
     8461 acagaagttg aaacggtcga atttgaaaac cgtgaaacag ctgatttgat tgattcgtta
     8521 tatgatatct tagaacggga aatggaagct cagagatatg taaaaactaa ccaatcaacg
     8581 attgcagagt atatcaaaca tacaacaaag aataaccgtc aattattgat tgagctggat
     8641 cacacatctc aaagctatac attgaaccac aatgaaattg gccgtgtacg aggtttccaa
     8701 accgaagtgg aagaaatgga acgtcaaaat gaacagatga ttccacagat ccgtcaacac
     8761 gaaattcctt attcagaagt gcgcacgttc tataaaacag tcttcaaagt cttagaagat
     8821 atcgaaaagc aacaagtaga gatcgacgac tcattacatg aattgagaaa aggcgaaaaa
     8881 gaagcgcaag aaaagatcga tacattcgaa ttcaaattgc gcagtttgaa acgttttgta
     8941 gaaaaacaac gtttgcctgg cttgccgaat gattacctag aattcttttt tgttgctact
     9001 gaccgtattg aagaactaag tgttgtcttg aataaaattc gagtaaacat ggaagaggtc
     9061 aatcgtttag ttgctctatg tgaagaagat ctagaattat tagataagaa gacacatgat
     9121 ttagtagatg cagcagcatt aacggagcaa atgctccaat atgcaaatcg ttatcgtcat
     9181 acacacgaag aagtgagaga agcaattgat aaaagctact atttattcaa taaagaatat
     9241 cactatcaag aagcattaga cgaaatcggt acagcattag agcgagtaga acctggtgct
     9301 ttcaaacgca tcgaagattt ttattttaat catccagact tagtataaag caaaaaagtt
     9361 agtaaaccag caagagcaag acggattatt gtcttgttct tgtctttttt tgtatatttg
     9421 aaaattgaaa caagtgaaaa tttcacctgc tcttgaaagc accttggaca accgttataa
     9481 tagaaaagaa tttcaaaaaa gaggggtttc ttatgatata ttttgataac agtgcaacaa
     9541 ccccaatctt tccgcaaagc ttagatgcat atgtaaaaac cagtcagcgg attattggga
     9601 atccatcaag cttgcatgat ttaggcaatc aagctaaccg gttgttgcag caagcaagaa
     9661 agcagatcgc tgatttgatc catgtagaac cagaagaaat ctattttact tcaggcggaa
     9721 cagaaggaga taactgggta ttaaaaggaa cgatgatcga aaaaagagaa tatgggaatc
     9781 atatgatcat ttcaggagtc gaacatccgg ccgtcagcga gacagcagaa caattaaaag
     9841 aattaggaat cgaagtaagt atcgccccag tggataaacg cggatttgta cgtgtcgacg
     9901 aattgaaaga attaataaga aaagagacag tattagtctc tgttatggct gtgaataatg
     9961 aagtaggatc gattcagccc atccaggaaa tcagtgatct tttggatgat tttcctaaaa
    10021 ttcatttcca tgtagatgct gttcaggcga ttggaaaagt accattctcg caatggttga
    10081 cagaaagagt tgattttgca acattttcag ctcataaatt ccatggccca cggggaactg
    10141 gtttcattta ttggaaaaag ggtcgtcgtt tagcaccatt actgacaggt ggagggcaag
    10201 aaagaaatca gcgaagtggg acagaaaatg ttccaggtat tgtggctatg gttaaagctt
    10261 tacgtttatc attagagata aaggaacaaa aacctgagca gacacaacgc ttaaaagtag
    10321 ctctaatcaa tgcgttgaag acgtatgaga aagtgacgat tttttcagaa ggaactgact
    10381 atgcaccgca tattttatgt tttgctctaa aaggaattcg cggcgaagtt ctcgtccatg
    10441 ccttagaaga aaaacaaata tttgtttcta ctactagtgc ctgctcctca cgtaaacata
    10501 tggcaagcag tacattgcac gcaatgcatg taccaaatga tctggctaca tctgctgttc
    10561 gaatcagctt agatgaaaac aattcgcttg cagaagtcga gcaatttatg attattttca
    10621 agcagcttta taagaaattt tctgcaatca attaaaagag gagtgttctt gtgaaataca
    10681 gtgaaattat ggtccgttat ggggaacttt cgacaaaagg aaagaatcga aaaagcttta
    10741 tcatgcaact agcacaaaat gtcagacaag ctttgaaaga ctttccagaa atcaaaatcc
    10801 atgctgatcg ggaccggatg catcttttat tgaatggcgc agacgggcag ctagttattc
    10861 caaaactagc aaaaatattt gggatccaaa atttctctcc aagtattcgt gtagaaaaag
    10921 atgtcgaagt tttgaaacaa gctgttcaag acatcatgaa agaaatttat actgggaaag
    10981 aaacatttaa aattatcgct aaaagaagcg atcaccaatt tgaattgaca agtaatgaat
    11041 tgaatcaaac attaggtaat gctgtttttg atattttccc gcatatccaa gttcaaatgc
    11101 gtcaaccaga tattccatta cgtgtagaga ttcggcgtga tggcgcttat ctatcgtatg
    11161 aaacgatcaa gggagctgga ggattgcccg taggaacaag cggaaaagga atgcttatgc
    11221 tttctggggg gattgattct cctgtagcag ggtactttgc catgaagcgg ggagtggaaa
    11281 ttgaagctgt tcattttgct agtccgccgt atacaagtga acaagcactg caaaaagcaa
    11341 aagatctaac agccaaacta gcaccttatg tgggaacgat ccattttatc gaagtgccat
    11401 ttaccgagat tcaagaagaa atcaaaaagt cagtgccaca aggatattgg atgacaatta
    11461 ctcgtcgaat gatgttgcgt ttgaccgatg aaattcgccg tattcgtcat ggtttagtca
    11521 ttttgaatgg tgaatcatta ggtcaagtgg cttcacaaac tttgcaaagt atggtggcta
    11581 tcaatgaagt aacgaatacg ccaatcattc ggcctgtagc gacaatggat aaacttgaaa
    11641 tcattgaagt agcagaacag atcgatacat ttaatctttc tatccagcct tttgaagatt
    11701 gctgtacgat ttttgctcct ccacagccaa aaacacgccc tcaactggaa aaggtccatc
    11761 aatatgaaga gcgtttagca atagatgaaa tgatccaacg agcactagct ggcttgaaga
    11821 tggaagagat acaagcagaa tcgtctaatg aaaaaactga agaattttca gaattgctat
    11881 aagaggttat gaaaatagtg ttttgaccag agtattagag aaacaaaacg aaaaaatagc
    11941 tccatgtatt ttaccgtttt tgaactaatg tcgaagatta gctgattgaa taccatttat
    12001 caagaagtta tggaaaagcc ggttcttttt tactaactga aaaataaaat tttatttaga
    12061 gatcaagcta cagttgataa atgatataaa aaagagaatt tcccatgaaa tagcgaggtt
    12121 gtgacagaag tgggcagctt caagaaataa gaaggaattt acgaaaattg cttttcaaat
    12181 ttttgtgaaa tttcggctta tttttcgaag aagttacttc tggaacaccg tgtatcagag
    12241 gttgcgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
    12301 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgttttattt
    12361 gtctcacacc cttttccttc ctacttgtaa ccttgctccc gtgttagaaa catgttacac
    12421 taaatatgtg aaattattaa caaggagcgt cgttatgcga gaaaaaacag atacactgcc
    12481 aaaagcaact gcgaaaagat tacctttata tttacgctac ttgaagttgc tggatgactc
    12541 aggtataagt agaatcaaat caaatgagtt cagcgaaatc acccaaattc cttctgcaac
    12601 gatccgcaga gacttctcgc aattaggcga gctaggcaga agcggatatg gctatgatgt
    12661 cccttttctt atcgatgtat tcaataatat ccttaatact aaagaagaaa aacgaatcgc
    12721 attagtagga tatggaaatc ttggcaaagc attgaaacac aacaatttta gaagaaatga
    12781 aaatctaaat atcgtgtgcg tctttgataa tgatccagca ctgatcaatc gagtaattga
    12841 tggagaaatg atctatccga ttgatcgttt cgcggagata gccaaagcaa aaaatgtgac
    12901 agtagcaatc tctacggtac caagtaaata ttcacaatct gctattgatg aaattgtcaa
    12961 aggaaatgta accgctatac ttaattttgc gcctgacaga gtaactgtcc cagcttatgt
    13021 caatgttcaa tacattgatt tgacaaccga gttgcagacg cttatttact ttgatgagaa
    13081 ttactcagaa gttttttctt aggaaaattt gagtaggctt attgatcttg cgagaactcg
    13141 tcgtaattct ttcaaaaatt ttttagtact aaatgaagtg gttttttcac gaccacttgt
    13201 tgactaaaaa aaaaaaccat tgtagaatga agcataacgc tgaacaagag gagtaactgg
    13261 cacattcatt cttagagaga gaactatctg ctgaaaggtt ctcaatgaca accagtgaag
    13321 gtagcttggg agtttctgtc ttgaatggag tagagataga cgagtcgtga tcgttaactc
    13381 acttgagagg caataatgat tgattattgc aatttaggtg gtaccgcgta aatcatacgt
    13441 cctagaatac gaaagtattt taggacgttt tttagtttaa aggaggaaaa cagcatgacg
    13501 gaagaaaata acctatcaac aaaatataat ccgcaagaag ttgaagctgg acgttatcag
    13561 cattggttag aacaagattt gtttaaaccg agcggagata aaaaagcaaa accttattca
    13621 atcgtaatcc cgcccccaaa tgttactgga aaacttcatt tagggcatgc ttgggatacg
    13681 accttgcaag atatgctgat tcgtcaaaag agaatgcaag gatatgatac tttatggctg
    13741 cctggtatgg accatgcagg aatcgcgaca caagcaaaag tagaagcgaa gcttagagag
    13801 caagggattt ctcgttatga tctaggtcgt gaaaagttca tcgatcaagt gtgggaatgg
    13861 aaagaagagt atgcgagcca catccgcgaa cagtgggcta aaatgggctt atctttagat
    13921 tacagtaggg aacgttttac attagatgat ggattatctg aagcagtacg taaagttttt
    13981 gttaccttat acgaaaaagg tttgatttat cgtggtgaat atatcatcaa ctgggatcct
    14041 caagcacgaa cagctctatc tgatatcgaa gttattcata aagatatcga aggcgccttt
    14101 tatcatatga gctatgaact agcagatgga tcaggcgtag tagaaattgc gacaacccgt
    14161 ccagaaacga tgttgggaga tacggcgatt gctgttcatc cagaagacga gcgttatcaa
    14221 gcgctcatcg gcaaaaaagt gattctacct ttagtcaata aacaaatccc aatcattgca
    14281 gatgaatatg tagatatgga attcgggaca ggtgtagtaa aaatcactcc agcacatgat
    14341 ccaaacgact ttgaggtagg taatcgtcat gatcttccac gcgtaaatgt tatggatgat
    14401 gatggtacga tgaatgaact agcaggaaaa tatgctggaa tggaacgttt tgctgctcgt
    14461 aaagcaatcg tgcaagatct gaaagaaatc ggtcgattga ttaaaatcga tacgatgatc
    14521 cacagtgtgg gtcattccga gcggacaggt gtcgttgttg aacctcggct gtctactcaa
    14581 tggtttgtaa aaatggcgcc gttagcgaaa aaagccattg ataaccaaga aacagatcaa
    14641 gcagtggaat tcttccctcc aagattcaat caaacgtttt tacgttggat ggaaaatgtc
    14701 catgattggg taatctcacg acaattatgg tggggacatc aaattcctgc atggtatcac
    14761 aaagaaacag gtgaaatgta tgtagggatg gaagcaccag cggatagtga aaactgggta
    14821 caagatcctg atgttttaga cacttggttt agttcagctt tgtggccttt ctcaacaatg
    14881 ggctggccag atacggaaag cgaagattat aagcgatact tccctactag cactttagta
    14941 accggttatg acatcatttt cttctgggta agcagaatga ttttccaaag cttggaattt
    15001 actggcgaac gtccatttga gaatgtcttg attcatggat tgattcgtga cgaacaaggg
    15061 cggaagatga gtaaatcatt aggtaatgga atcgatccga tggaagtaat cgaaaagtat
    15121 ggagcagatg ctctccgctg gttcttatcg aatggttctg caccaggaca agatgtccgc
    15181 ttcagctatg aaaaaatgga tgcttcttgg aactttatca acaaaatctg gaatgcttca
    15241 cgtttcgtta tcatgaatat cgaaggaatg accgtagaag agatcgattt atctggagaa
    15301 aaatcagtgg ctgaccggtg gatccttact cgtttgaatg aaacgatcga acgagtaact
    15361 gagctatttg atcgttttga atttggtgaa gcaggtcgac aactgtataa ctttatctgg
    15421 gatgattttt gtgactggta catcgaaatg agtaaagaag tactttatgg agatgatgct
    15481 gtttcaaaac aaaccacccg tagcatctta gtccatacac ttgatcagat cctacgttta
    15541 ttgcatccaa tcatgccatt cgttacagaa gaaatatggg aacatattcc tcatcaggga
    15601 aattctctag tagtggcaga atatcctgtt gtacgtccag agtttgatga tgaaacagct
    15661 tctaaaggga tggaagtatt gaaagaattg attcgagccg ttcgaaatat tcgttcagaa
    15721 gtgaatacac cattgtctaa accaattacg ttgctaatta aaacaaacga tgctaaaatc
    15781 gatcaatttt tagttgaaaa tacaagctat atcgaacgct tctgtaatcc cgaagagttg
    15841 acgatatcaa gtgagatcac tgcgccagat ctagcgatgt cagctgttct gacaggtgca
    15901 gaaatcttct tgccgttagc aggattgatc aatattgaag aagaaatcaa acgattagaa
    15961 aaagaactgg aaaagtggac aagcgaagta aaacgtgtcc aaggaaaact ttctaatgaa
    16021 cgttttgttt ctaacgcgcc ggaagaagta gtagaggcag agcgtgcaaa agaaaaagat
    16081 tatttagaga agcaaacggc tgtcaaagaa cgaatcgaaa gtttgcgtac tattcaataa
    16141 gaatagaaaa agagagtgtg aaatcagcta gctattacta aaagctagct gattgaacat
    16201 cgttacagga aagagtttga gcctcactct tagcttcgat tcataaggag atgtgacata
    16261 aattttgtcg catctctttt ttagtctact cttattatgt aggagaagga gggaggtgga
    16321 tcatgtatca agagtcaata aagtagttga taaaatcgtt cctctctctg tttcttttct
    16381 ctttgtcata cttttatttt tcttatgtgt tctatttgtt ataataaaag agaaatgggg
    16441 gaattgttga tgacgattga agaagcaata gaatggatcc atagtcgatt accatttggt
    16501 tcacgtcctg gccttgaccg agtcgaagct ttattagaag tggtagggca tccagagaga
    16561 aaagtaaaaa cgatccatat cgctggaaca aacggaaaag gttctactgt agcttatttg
    16621 agaagcctat tagaagaggc tgggctgaca gtaggtacat ttacttcacc ttatatcgaa
    16681 acgttcaatg aacggatcgc aatcaatggc aaaagcattc cagacaatga tttgatccag
    16741 cttgtaaaaa aatatcagcc actgatcaaa caacttgatc aatttgaagc agtagctggt
    16801 attactgaat tcgagacatt aacagcaatg gcatttgatt attttttgat gaagaaagtg
    16861 gacgtagcga ttatcgaagt tggcttaggc ggcttattgg attctaccaa tgtagctgta
    16921 ccaatgctga cgggaataac aacgattgga cttgatcata ccgaaatact tggagataca
    16981 attgaagaaa tcgctgcaca aaaagctgga atcatcaaac aaggcgttcc agtagtaaca
    17041 ggaaatattg ccccagaagc ttttactgtc atccaacaaa cagctaaagc aaaaaatgca
    17101 ccgctttatg tatacgggga aaattataaa ataaactatc tgcatcctga tgagatgtgg
    17161 ggagaagtat ttgaattttt taatgaaata ggaaagatcc ctcaattaac cactccgctt
    17221 ttagggcaac atcaagctga aaatgcagga gtagccatcg aattgtattt tgtttactgt
    17281 caattggaaa agctgccttt ccgagaaaaa gatgttttga agggattgaa gaaatctcga
    17341 tggccaggaa gaatggagcg tttgagtaac gagccattag ttgttttaga tggtgcccat
    17401 aatacgcatg caatgaaccg gcttgttgag aatttgaaaa aagaattcaa aggatatcaa
    17461 atcaacattt tgttttccgc cttacgaact aaaaatgtta gagaaatgct agaacgattg
    17521 cagcaagtac caaatgtcca tatttatttg acgacctttg aacatccaaa agccatcgaa
    17581 ttaagtgaag tatcagattt agcaagtgag agagtatcaa tcgtttcatt gtggcaattc
    17641 ggattagcag atatattgga aaagatgaca aatgaagacg tattagttgt gaccggttca
    17701 ctttatttca tttcagaagt tcgccattta ttaatacaat taggaggaga tcatgaagta
    17761 taatgcagtg atttttgaca tggatggttt gctgttcgac acagagttag tgtattacga
    17821 agccacacaa gtagtagcag acaagatggg ttttccttat gataaagacc tgtatttaaa
    17881 attcttaggt gtttcagatg aagaagtatg ggcgaattat catatgattt tctcggaatt
    17941 tggcaaaaca agtgtacaaa gattcataga tgattcatac caggaaacgc ttgaacgttt
    18001 ttcgcaagga gcggtacaaa tgaagcccgg tgtgttggaa ctcctctctt ttttagaaga
    18061 acatcagatt cccaaagttg tcgcttccag taaccaacga gcagtcattg aacttttatt
    18121 gaagaagaac aatctgtttg aacgatttga gacgattgtt tctgcagaaa atgttcaacg
    18181 agcaaaacca gacccagaaa ttttcttgtt agctaatcag aagctaggta cagcgaagaa
    18241 agaaacattg attttagaag attcacaaaa tggtatttta gcagcttctg cggctgagat
    18301 cccggtagtc atggtaccag atctgcttcc accatcccaa gaattgatca aaaaaacagc
    18361 tggggttgtt tcttctttac atgaaatccc tgcattgtta aaataaaaat ccatctcttt
    18421 tcgtatctta ttttagataa gatagaaagg agatgttttt ttgggaaaac gattgatcaa
    18481 agaagtacct actagctctt taccaaggga acgtatggaa atttatggcg cggaagcttt
    18541 atcggatcag gagcttttgg caatcttact gcgtaccggt caacaccctt atagtgtgat
    18601 gtcgattgct ggaaatctgt taaaaacgtt tggaggactg gcctctttac gacaggctac
    18661 tttgcatgag ttagaagaga ttcgagggat aggacgcgtg aaagcaatcg aaatcaaagc
    18721 cttgatcgaa ttaggtcgac gaattcagac ggatcatgaa agagcggctc caccggttcg
    18781 taccagctac gaattggccc agcaattgat tctggaaatg aaagatcaca aacaagaaca
    18841 tttggtttgt atttatttgg acacgaagaa tcaagtgatc ttaaaaaaaa cagtgttcat
    18901 cggttcgctg aatcagtcag tcgctcatcc tagagaaatt ttccattatg ctgtacgtta
    18961 ttgtgcagca aggatcgttt tagcgcataa tcatccttct ggtaatgtta ttccatcgca
    19021 gcaggatatg aattttacca aaaggataca aaagtgtgga gaaatgatgg ggatcactgt
    19081 actggaccac ttgatcatcg gacgcaaacg atattttagt ttgagagaag aaggaatgat
    19141 ggaagagaag taagttgctt tcttgcaaaa aaaatgaagc aagtgtacaa ttatattgta
    19201 attttgtttg ggaatagaag ttgaaacatc tgttgcacct ctatcgtaca cgagcaatgt
    19261 tacaatttaa attagtgctt ttggcacgag gaggatatta ttatggaaaa cggaacagta
    19321 aaatggttca acgctgaaaa aggatttgga tttatctcac gtgaagatgg aagcgacgta
    19381 ttcgttcact tctcagctat ccaaggtgac ggattcaaaa cattagaaga aggtcaagca
    19441 gtgactttcg acgttgaaga atcagaccgc ggcccacaag ctgcaaacgt tgtaaaagct
    19501 taattttatc tgtaaatcaa attgatggta gacatcaatt tttacgagag aacaaagcga
    19561 gtaacaaaaa tacctgccac aagagacaat ttctcttgtg gcaggtattt ttttgtatga
    19621 aaaagtaaat aaatttaaga ggctatgaaa gagccacctt gtatcaagga ataagaacaa
    19681 acaaaaatgg cttctcatgt ttttcatttg ttttctctta tattcaggac aaggtgctga
    19741 tttcataacc tcttatttat tcttcatctg gtgtcattac cattggcaaa atcattggat
    19801 gtctttctgt acgttcgaat aagaaaggct gaagcgcgcc agtcatcgct tctttcaatt
    19861 tatattctga acagtcaggt ccattcaaac attctcttaa agcgtggaat aagattcgct
    19921 gagcttcatt gatcatttct cctgattctc gcatgtagac aaatccacga gagagaatat
    19981 ctggaccagc caagatttct tttttcttga tatccactgt cgcgacagct aatacaagac
    20041 cttcttctga aagcaggtga cggtcacgta aaacgacatt accaatatca ccgatcccgc
    20101 taccatcgat atacacatct ccagccacaa aatgcccagc aggacgcgca gaatcgctag
    20161 ttaacgctaa aacatcgcca ttttccatga tgaagcagtt ttctgcagga acacctacat
    20221 cttgtgctag agaagcatgt actttcaaca tgcggaattc tccgtggaca ggaataaaat
    20281 attttggctt catcaaacga agcattaatt tttgttcttc ttgtccaccg tgaccggaag
    20341 tatggatgtt attgattttt ccgtgaatca cttctgcacc agcttcagac agcaaattga
    20401 tcaaatgatt gacacttgtt gtattaccag gaataggtga acttgaaaag atcactgtat
    20461 cgtctggctg gatttggatt tgacggtgag taccattcgc gatgcggctc aacgcggcca
    20521 tcgcttctcc ttgagaacct gtacatagga tcatcgtttc attggcaggc aagcgattga
    20581 tttcgtgtgc atctacaaat gtgccgtctg gaactttgat atatcctaaa cgttgaccgt
    20641 tgacgatggc attttccatg cttcgtccga aaacagcgat tttacgacca gttgccacag
    20701 cggcatctgc tgcttgctgc aaacggaaga tgttagaagc aaagctggca aaaatgatac
    20761 gtccttcgat ttttgaaaag atcttcaaaa tagaatgccc aatcgttttt tctgatttag
    20821 taaatgttgg aatctcagca tttgtactat cagataaaag gcacaagact ccttcttccc
    20881 cgatacgagc cattttatgc aagtttgctg gttcgccaac aggagtgaaa tcgaatttga
    20941 agtctccagt cgctacgata tttccagaag gtgttttcac cacgatccct aatgcatctg
    21001 ggatactatg ggtcgttctg aaaaagctga cagaagtctt tctaaaacgg atgatcgtat
    21061 cttcattgat ctcatggagc tctgcatctc gaagcaagcc atgctcatct aatttgtttg
    21121 tgatcaacgc taatgcgaga ggaccagcat agataggaat atttgcttgt ttcagcaagt
    21181 agggtattcc gccgatgtgg tcttcgtgcc cgtgagtaat caccaaggct tttactttgt
    21241 gtaagttttg tatgatgtag ctgtagtcgg gaatgacata gtcaattcca agtaaatcgt
    21301 cttccgggaa tttgatcccg gcatcaatca aaatgatttc atcttggaat tgtaccccat
    21361 acgtattttt cccgatttca cctaatccac caatagcaaa gacgcctgtc tcgttatttt
    21421 tgatggaaag tttcatatta aaactccact aatgagaaat cagcattttc tttttcgtac
    21481 tctagatgac ttccttctag aagttgaacg aattcaatat tgtatggggt attttcttcc
    21541 acacgttggc gagcgtctac atcgcttttt gcctcaatgt aaagagattt tgtttcttcc
    21601 cgtttagggt tgcgtacttt tgtttcttga taataaactt tatagatcat tgttttgtct
    21661 cctttatctg cgtaaagcag tgatttagct agttgatttt tttagacaac acaaaacgag
    21721 gggacaagcc acctggtttt tcatatgctt tgactagaat actggattcg agtcaaagag
    21781 acaagcctcg tttttgctta tctgttcgga tattttttca accgtacctt ctgtataaaa
    21841 aatatacaac aattaattgt tattctagca taaa
//
LOCUS       DACCXM010000044        21854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCXM010000044 DACCXM010000000
VERSION     DACCXM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.046"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(541..1566)
                     /locus_tag="IVY61_001360"
     CDS             complement(541..1566)
                     /locus_tag="IVY61_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7970482.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1841..3526
                     /locus_tag="IVY61_001361"
     CDS             1841..3526
                     /locus_tag="IVY61_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP7970483.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3540..4823
                     /locus_tag="IVY61_001362"
     CDS             3540..4823
                     /locus_tag="IVY61_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7970484.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4893..5762
                     /locus_tag="IVY61_001363"
     CDS             4893..5762
                     /locus_tag="IVY61_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7970485.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5759..6595
                     /locus_tag="IVY61_001364"
     CDS             5759..6595
                     /locus_tag="IVY61_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7970486.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6621..7688
                     /locus_tag="IVY61_001365"
     CDS             6621..7688
                     /locus_tag="IVY61_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP7970487.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7685..8512
                     /locus_tag="IVY61_001366"
     CDS             7685..8512
                     /locus_tag="IVY61_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7970488.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8557..9582
                     /locus_tag="IVY61_001367"
     CDS             8557..9582
                     /locus_tag="IVY61_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7970489.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9579..11762
                     /locus_tag="IVY61_001368"
     CDS             9579..11762
                     /locus_tag="IVY61_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7970490.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11762..12892
                     /locus_tag="IVY61_001369"
     CDS             11762..12892
                     /locus_tag="IVY61_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7970491.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12889..13566
                     /gene="pgmB"
                     /locus_tag="IVY61_001370"
     CDS             12889..13566
                     /gene="pgmB"
                     /locus_tag="IVY61_001370"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7970492.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13650..14177
                     /gene="msrA"
                     /locus_tag="IVY61_001371"
     CDS             13650..14177
                     /gene="msrA"
                     /locus_tag="IVY61_001371"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7970493.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14378..15064)
                     /locus_tag="IVY61_001372"
     CDS             complement(14378..15064)
                     /locus_tag="IVY61_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP7970494.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15144..16682
                     /locus_tag="IVY61_001373"
     CDS             15144..16682
                     /locus_tag="IVY61_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP7970495.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16802..17269
                     /locus_tag="IVY61_001374"
     CDS             16802..17269
                     /locus_tag="IVY61_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP7970496.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17788..18231
                     /gene="rplM"
                     /locus_tag="IVY61_001375"
     CDS             17788..18231
                     /gene="rplM"
                     /locus_tag="IVY61_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP7970497.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18247..18639
                     /gene="rpsI"
                     /locus_tag="IVY61_001376"
     CDS             18247..18639
                     /gene="rpsI"
                     /locus_tag="IVY61_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7970498.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18727..19953)
                     /locus_tag="IVY61_001377"
     CDS             complement(18727..19953)
                     /locus_tag="IVY61_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7970499.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20025..20183)
                     /locus_tag="IVY61_001378"
     CDS             complement(20025..20183)
                     /locus_tag="IVY61_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7970500.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            20875..21594
                     /locus_tag="IVY61_001379"
     CDS             20875..21594
                     /locus_tag="IVY61_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7970501.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            21596..>21854
                     /locus_tag="IVY61_001380"
     CDS             21596..>21854
                     /locus_tag="IVY61_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7970502.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKS"
BASE COUNT         7090 a         3850 c         4523 g         6391 t
ORIGIN      
        1 gtctaaaata atttgtctaa ctagaagaaa agtgggaaag gatcttaaca acatcggttc
       61 gtatccaatt aaggtatcgg aacgatcatc ttccaagaga agtcaatcaa caaaagtaaa
      121 gatagatcat tttttgaatg caaaataaat tgatgggaag atagagcggt tatttttaat
      181 attttgctag ctattattga ataattcctc gaataaaaca aaaccagtag acatgtctgt
      241 tattctattt atgttttaga gataagtatc cccaaataac atggattgaa ataagggtaa
      301 atgtgtttaa aaaaacgaac tataaaaact tatacacaac gtggttaaaa atagtttttt
      361 cttccctgtg gaaaaaatag tttaacacaa acattatagt tatccacagc tatctaaaac
      421 agatggaaaa taagaaaaat tcgctaaaat accgggaggg ggtaacgagg gatgagtgat
      481 tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc tgttctttta taattctgtt
      541 ctacatttct tgttgttgga tcgaatctgt gagagtaatc tcggttgaaa gaatgattct
      601 tttttcttca gggtattggt ggtggattag ttggaataaa agttctgtgg ctttgattcc
      661 taactgattt gtcggaatgt ctatccgagt caactgtggc gaaaataggg aggacaataa
      721 gggatcactg aatcctgcga tcgaaagatc atcaggtatc tgctttccag attgttctgc
      781 cactttgtaa atactcaatg attttaatgc atcggtagaa aaaatcgcag ttggtctttc
      841 tcgtagcttc attaaatcat tcattgctgc catgctatct tctaagcgaa agccgccgtc
      901 tataatccat tcattacgga tatctaggtg gtgatcaaat aagcattgac ggtaaccatt
      961 gagacgatca ttggatacat aaacttctgt cggagcatgg agacagccga tatgacggtg
     1021 tccattcttg atcagtgttt ccaccaaatc gtaactatcc tggaaattat ccgtgtccac
     1081 tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt accacaatag gaatattgta
     1141 ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag cttaaaataa taattcctcg
     1201 gatcattttt tcttggatct ttttcgcgca tttttctagc tcttcttctg gtgtttttgc
     1261 cgtttgtaga atcaagtcga agttattttg ttcagaaacg gcagaaatgg attgaataac
     1321 atctgaaaaa aaaggattgc cataggtcgt atgagaagta cgatgagata atgctagaat
     1381 cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg ctcggttcat aattcaattc
     1441 tttgattgct tgaagtacac ttgcttttgc tttatcagag atatttggct ggttatttaa
     1501 tactctggag acagttgatt ttgagacatt agcttttttc gcaatgtcat aaatagtagg
     1561 tttcaatttt atatgcacct cctttttggg agcgttttag aataattcca tagtacaata
     1621 gacattcttt tttgtctatg attttgaaaa tttaggaata gcttgcatga tcatgagaga
     1681 ccatattttt ttaagaagct attaattatt gatacataag ctttcttatt tgtatttttg
     1741 tgtatatcaa aattatttaa aaaaatgcta ttgacagtaa gcgttttcca taatatgatc
     1801 aaatcgttgg gaacgctccc aaaaaagaaa ggggaatccg atgtatacaa aacttttaca
     1861 agcgatttat cgcgactcag atttgtctga gaaaaccgcc tctatgattg aacaaaaagt
     1921 cagtgtttat cagcagaaga aaggccaggc aggcaaactg cattgggacg aagaagatat
     1981 tttcttgatt acttatgggg atcaattttt tgaagagcag caaccaactt tgcgtacatt
     2041 taagaaattt tatcaaaagt atttggcaga tacatttaga gttgtacatt tcttgccatt
     2101 tttcccatat acatctgatg atggtttctc cgttgtaaat tatcaggcaa tcaacgaccg
     2161 tttgggcaca tgggaagata tagaagaaat gagaagcgaa acacggttaa tgttcgactt
     2221 tgtctgcaat catatgtctg ccgaaagtac ttggtttcag gaatatttaa acttgaatga
     2281 tacgtacaaa gattttttta ttgatttgcc actggataca gatacaagca gcgtgactag
     2341 accaaggacc agcccgttac ttacaacgtt cattgacaaa aacgggaaag aaaaacacgt
     2401 atggacgact tttagcgaag atcaagtaga cttgaatttt aaaaatccag aagttttatt
     2461 gaaaatgatc gatattcttt tgttttactt agaaaaaggc gcttcattta ttcgcttgga
     2521 cgctgtggga tttttatgga aagaactagg aacttcttgt atacacctgc cagaaacgca
     2581 taaaatcatc caattattcc gtgcaatcgc tgatgaagca gctccaggaa cggtattgat
     2641 cactgagaca aatgtgccac acaaagataa tatcagttat tttgggaatg gcgagaatga
     2701 agcacagatg gtctatcagt tttcgctacc acctttgacc cttcaagcga ttcgttcggg
     2761 taatacaagt aagttgaaag aatggttaaa aaccatcgat ttcgcatcga aaaaaacaac
     2821 tttttttaat tttttagcct cccatgacgg tatcggattg aatccggtac gaggtattct
     2881 gccggaaaaa acgatccttg aattagtgga acagctgaag aaagagggta cccgagtaaa
     2941 ctacaagaaa aacccagatg gaacgaccag tccatatgag ataaacgcta cgtatctaga
     3001 tgcgttaagt tctccaacag atacgacgga atggagaagc aaacgctttt tagctgctca
     3061 ttcatttttg gtaacgttac caggcgtgcc ggcaatttat attcaaagtg tgctgggaag
     3121 tcgaaatgat tacaaaggcg tagaaaaaac aggagagaat cgaaaaatca atcggcaaaa
     3181 atttttgtta gcagatattg agaaagagtt gcagacagat ccattaagaa cagcagtata
     3241 tggtgaattg acccagatgc tatctattcg aaaaaaagaa aaagccttcc atccgaatag
     3301 tttgatttac ttactggact cagcagattc ttttatcagt tttattcgcg aagcgaagga
     3361 agagaaagtc ttagttatcc ataatttttc caatcaatca gcaggttatc ttttaccaaa
     3421 aggagattac ctagacctgt tttctggaca aattatccat tgcagtcaag aacgaaagtt
     3481 gatattccag ccgtatgaat atatgtggct gaaaataata aaataagagg tggaaaaaaa
     3541 tgaaaaaaat tattgttact acctgtctgc tagctactgg atttcttctt actgcttgtg
     3601 gtgaaagtca aagctctaac gaatcacaac agacaacgat tcaattcatg cactcttctg
     3661 tcgaacagga acgattaagt gtgatcaatg aactggttgc tgattttgaa aaagaaaatc
     3721 cagacatcaa aatcaagcaa gtgccggtag aagaatcagc gttcaataca aaaatcgtta
     3781 cgttagctcg ttccggtaaa ttaccagaag taatggaagt cagtcaggat tttgctaaag
     3841 tgatggataa agatgaactg attgatcaga cggcagtcca aaacgttatc gaggaagctg
     3901 gagaagataa ttattatgac ggagccaaaa accttgttcg ttcagaagat ggaaaaagct
     3961 atattgctgc accaattagc ggctgggttc aaggtatttg gtatgacaaa gaaaaattat
     4021 cagaagctgg tttttcagaa ccagaaaatt gggatgatat tttaaaaatt gctcaacatt
     4081 tcacagataa agagaataaa caatatggaa ttgctatgcc tactgctgaa ggaacaatga
     4141 gtgagcaagc gttctctcaa tttgctttgt caaatggagc aaatgttttg gatgatgaag
     4201 gcgaggtaac gatcgatacg gaaaagatga gagaagcatt gtcgttttat caaaacttat
     4261 cccaatacac gatgccaggc tctaatgatg tgacagaaat caaagatgct ttcatgaatg
     4321 gaacagcacc aatggcaatc tattcaactt acattttgcc ttctgtttat gaagcgggga
     4381 actcagaaaa tatcggtttt gctatcccaa cacaaaaaga tcaagctgtc tatggcacag
     4441 tatctggatt gacgatttct tctggtctag acgagaaaca aaaagaggct gctgaagcat
     4501 tcacagcttt cttatctcaa ccgaaaaaca tggaaaagtg ggtcttgatg tctcctggtg
     4561 gtgctcaacc agtgaataaa caagtagtcg aactggatgg ttacaaagaa aatgaagtca
     4621 tcaagtcttt tggcgagctg ccatctgaga ttgctagtgc ttttgatgaa gtacaagtct
     4681 ttggactagt cggggaaaag aatttcacga aaatgggaga tatcacaagc tcaggtgtga
     4741 tcggaaaagc agtgaaccaa gtaactgtcg gaaacgaaga tgtcgatcaa gctttagctg
     4801 acgcacaaaa aaatacgaaa taacaagaag ttctgccaat ggtgttcgaa aaaagtagat
     4861 agctattggc agattatcag ggggaggttt caatgaatcg tccaaggaaa cattctgact
     4921 taaaattagc catgggactt ttagcaccaa gcatgctttt attagctgtt ttagtgctga
     4981 ttccaatgct ttccaatatt cagatcagtt ttttcgatat gccgctgaat cctaactcag
     5041 acgccgtttt tatcggatta agcaattatc ttgaaatttt aagtgatagt gaattttatc
     5101 gttctttagg tttaacagtt gcttacacat tcttagttgt agcaggcagt acagctttag
     5161 gattagccgt agctattttg cttaaccgca agttcagatt taggaaaacg gtgcgttcac
     5221 tgattttatt aagttatgtt acgccatcta tttctttgat ttttgcttgg aaatatatgt
     5281 tcaataatag ttatggaatc gtcaatgatc tgttagtaga taggttgcag cttttcaacg
     5341 aagcaccgct gtggttcgac cagccagttg ctagcttctt tatggtcgtt ttgtttgcta
     5401 tctggagata ctatccgtat gcgtttattt catttttggc tattttgcaa acagtcgaca
     5461 aaacgcttta tgaagcagct gaaatggacg gtgcaaatgc ttggcagaag ttcaagatcg
     5521 tgacactgcc ggcaatcaaa ccagtactag caactgtgat tgttttacga tctatctgga
     5581 tgttctatat gtttacagat gtctatctac tgacgaataa agtcaatatc ttaggcgttt
     5641 atttgtatca gacagccttt gcgttcaatg atcttggaaa agcagcagct atatcgattc
     5701 tattgttcat cattattgca gcggtcgtct tgctctcacg aaagaaggtg gacttgaaat
     5761 gaaaaataaa aagagcagga aaatcttatt ttacgcgacg attttgttgc tattagctgt
     5821 caatttgttt ccatttttga tcatgctgat gacatcattc aaggattcac aagaagcaat
     5881 tgcaacgaca caaactattt ttccgaaaga atggactttt gaacattatc aggatatttt
     5941 caatgctgag atttttccat tcatcatgta tttcaaaaat agtttagtga tctccttgat
     6001 agcctccgta actgcagtag tgattgggat cagcggggca tacgctttgt caaaactacg
     6061 cttcaaagct cgctcgaaaa tcaatgctag cttttacacc gtttatatgt tttccggtat
     6121 tttgatcgtt gttccattat ttaaaatcat ttccggtcta ggtttgtatg ataccaatac
     6181 agcactgatt atcacgatgg tcgttcaaac acttcctact gcgatattca tgctaaaaag
     6241 ttattttgac acgattccga ctgatattga agaagcagca atgatcgatg gtcttagccg
     6301 gatccagatc attattcgga tcattatacc attggctatt tctggaatca tttctgtctt
     6361 cgtttattgc tttatggttt cgtggaatga ttatttattt gcttcgattt tcctgtcgac
     6421 ctccgaaaaa tttactttac caatcggttt gaacacatta ttcagtacac cagactacat
     6481 ttggggacga atgatggccg cttcattggt gaccgcttta ccagtcatca ttatgtatgc
     6541 gttatctgaa cgatttatta aaggtgattt agtcgaaggc ggcgtaaaag gataaaaaga
     6601 aaagaaagag gaaagaaaag atgaaaaaat tagttgcatt agaaccaaga atagccggat
     6661 tcaaagatta cgaagaccgt gagatcgaaa agaacgaagt acgtatccga gtaattttcg
     6721 ctactccaaa accaggaaca gaaattgtcg actttagagg attaagtcca tttatcgatg
     6781 aagaattttc agaagaatgg cgcctattct tgccaagaga agaaggaagc caacgcggtg
     6841 tggtatttgg cgaatttcaa ttaggaaata tgattgtcgg tacgatcgaa gaaacgggca
     6901 gtttggtgac agagtatcaa aaaggagagt tagtctgcac ttatggaccc attcgtgaaa
     6961 cgctgattac aaacggtgtt gataattata aactacgtaa acttgatcgg ttggatcaat
     7021 ggaaaaatgc agtatgttac gatcctgctc aattcgcttt aggtggtatc cgggaagcac
     7081 atgttcgagc aggtgatgct gtagcaatct tcggtttagg cgctatcggg caaattgcga
     7141 ttcaattagc taaaaaagca ggtgctacta ccatcgttgg gatcgatccc atagcgcagc
     7201 gtagagaatt agcagaaaaa tacggtgccg attacacgct agatcctatg aaagcagatg
     7261 tcggttttcg tctaaaggaa ttgacagata aacgtggagt agattcagtt attgaaacga
     7321 gtggcagtgc tgccgcatta caagcttctt taagagggat cggctatggc gggatcattt
     7381 cttatgttgc ctttgctaaa ccttttccag ctgggtttaa tcttggtcgc gaagcacatt
     7441 tcaataatgc aaaaatcgtc tttgctcgag catctagtga accaaaccca gattacccaa
     7501 gatggaaccg taaacgaatc gaggacacat gttgggaatt attgatgaac ggttacttgg
     7561 attgtgaaga aatcattgat ccagttgtgc catttgcaga aagcgcagaa gcttacatgc
     7621 gttatgtaga ccagcaccca gaagaaagta tcaaaatggg cgttcaagtc cgaggaggag
     7681 aacaatgaaa ttcggtacgc aagatcaagc atttttccca agagacattt tagaaaaatt
     7741 tagatttatc aaagactgtg gatttgaaga atacgaaatc gacggtaagt tgttaataga
     7801 aaatatcgac acagtcaaac aggcaatcaa agaaacaggg atcccagtag tgacagcttg
     7861 taacggctac gatgggtgga tcggtgactt tatcgaagaa cggcgtctta atggtgtacg
     7921 tcaaattgct gagattttgc gtgcactaag cgaggttgga ggcaaaggaa tcgttatccc
     7981 agcagcttgg ggaatgttca cttatcgatt accgccgatg gtttcacccc gaagcaaaga
     8041 aggagactat aaagcagtca gcgaatcact tcgttattta gataaagtag ccgaagaaac
     8101 aggtacggtt atttatttgg agcctttgaa ccgataccaa gaccatatga tcaatttgtt
     8161 agcggatgcg cgtcattaca ttgactctaa tcaattgaaa catgtgcaga tcatcgctga
     8221 tttttatcat atgaatattg aagaagatga tatttcaaaa gcattgacta cgcaaaaaga
     8281 gtatgtgggt catatccatt tagcagataa tcatcgctac caaccaggat ctggcgcaat
     8341 tgattttact cgccattttc aaacgctact tcaaaatgag tatgaaggtt atatggtata
     8401 tgaatgtcgt ttgcgaggag aaaatgaagt gaaaatttat caggaatcat tgcaatactt
     8461 gaaaaactgt ctggaaacag cgcgtcattt aaatgattta gagaaagaat agagaaagaa
     8521 taacaataag taaataagca aaggagggag aaataaatga agaaagtagc aatcataggc
     8581 gctggacaag tagcagaaaa agtacatgct gcttattatc aaaaaagatc agatgtctta
     8641 gaattggccg ctgtggttga tccagtcagc cagcgagcag aagacttttg ccgacgtaca
     8701 ggattttctc atgcgtatac atcagttaca caaatgctga atgagatttc tcccgatatc
     8761 gtgagtgtct gtacacccaa tcgctttcac ttcgaacatg tattggctgc cctcgaagct
     8821 aatgcttcag tgatgtgcga gaaaccacca gctatgacag ctgatcaagc aagaaagatg
     8881 gcagagacag ccgataaaaa cgggcagatt ttggcttatg gattccagca tcgtttttct
     8941 gacgaagcaa caatgatcaa gaatcatctt gatgttctcg gggatattta ttacgtagaa
     9001 gcgaatgcgc ttcgtcggtc tggtgttcca ggctggggaa cgtttatcga caaagaactt
     9061 caaggaggcg ggcctttgat cgattatggc attcacatgc ttgatacagc gatgtatatt
     9121 actggatttc ctaaagtaaa aagcgttcgg gcgtacagct tccaaaaaat tggtacgaaa
     9181 aaaaattcag ggaaattcgg gaaatgggat ccaagcaaat tcacagtaga agatgcaatt
     9241 tttggcacac tggaattaga aaacggcaag atcatccaac tgaatacttc gtttgctttg
     9301 aatatcagag aagacaaact gaatgtggct ttttgtggcg atcaggcagg tgctaatctg
     9361 tatccaggcg aactattcga agatgtagat ggaaagctgg tttccttaca aaagaccacg
     9421 ccagttaatg acccacatga actaagtatg gctcattttg tgaatcgggc gctcggtgac
     9481 cagactcaga tgattgcaga tggctggcaa ggatatgtca tccagaatct ggtggaaaat
     9541 ttatatcgtt cggcagaaac aggagaagtt attaccttat gaaagtagaa gaaaaacagt
     9601 ttgatttaga gtatatcaat aagtatgcga gtcagatgac cattggaaat ggattttttg
     9661 gtatcagagg aagccaggag gaagactatc ggaatcaagt aaggggaatg tttgcagctg
     9721 gtgtatataa ccgaccttta ggagcagaat cagccgagtt agtgaatctc ccagatgtca
     9781 tcagatggga aataaaaatc gacggtgaac atttttctct tgagacgatg aaagttcttg
     9841 catacgagcg ggcactagat ttagaaatgg gagagcttaa acgctatgcg ctcattgaaa
     9901 ctgctcaagg aaaaaaagtg gaagtcatta cttgtcgtgt tgctgatcaa aataatctcc
     9961 atcaggcagc cctgaaagtg gtcatcaagc cgttgaccga agactgccaa gtagatgtga
    10021 aaacgggtat cgatggacag caaaccaatt ttggtacaca gcagttggtt gaaaaagaac
    10081 tgcgagtatt tggtgacgat ttgatcagtg catcctacca gacaacagaa agtaaaattg
    10141 atattgggat tgcagctaaa ttcaataaag caggtgtatt ccaagcaaaa aatcgccgga
    10201 tcaccgcttc cttttcagaa aagatcagaa aaaatacgtc ctttacgctg gagaagatca
    10261 tcgtgctctc tacctctctt cagcaaacag acccgtttaa gaaaagtatt gcccaagccc
    10321 agaaaagcgt tagttataca gaaattttag agcagtcgat gtcttattgg acagattttt
    10381 ggcatcagca tcggataacg atccaaggcg attcttcttt cgatcagtta gcgatggact
    10441 tcgctgctta ccatttacag attatgactc cccgcaatga tccaagtgtt agtgtagggg
    10501 cgaagggact aactggcgaa ggttataaag ggcatgtttt ctgggatacg gaaattttta
    10561 tcctgccttt ctttttatat cagtttcctg aagtagcaaa gaatttgctg ctgtaccgtt
    10621 atgatcgatt agaaggcgca cggcaaaaag caagagatta tcactatcaa ggtgcacttt
    10681 tcccatggga aagcgcacac actggtcagg aagagacacc caaatttgct gcattgaata
    10741 tcaaaaccgg aacaagacaa gaagttgctt cagctgccgc agaacatcat atcagtgcag
    10801 accttgctta tgccgttcaa gagtatgtcc aagcaacaga ggatcaatcg tttttagaaa
    10861 caaagggaaa aacgctcatt catgaaacag cagctttttg gctgagcaga gctgtaaaag
    10921 tcgatgatca tttagagata cacagagtca ttggtccaga tgaatacaca gaatatatcg
    10981 acaataatgc atatacgaac tatctggcgc attataatgt ggccttagct gttaaatatc
    11041 aagctggcga tccgcttttt ttgaaagaat gtcaaaattt tttagataag ttatatttgc
    11101 cagtgcctaa caaagaacag ctgattccgc aagatgacac gtatctcagt aagccagtta
    11161 ttgatttgac cacttacaaa gaacgtcctg gcagccagtc tattttactt gattatgccc
    11221 gtagcgaagt aaatgaactc caagtcagca agcaagcaga tcttgtcatg ctgttatact
    11281 tattacctga gtatttttct caaccaatcg tttctaagaa tttgcattac tatgaaaaca
    11341 aaacaatcca cgactcatcg ttgagtaaag cgattcacgc catagaggct tgccgtatcc
    11401 aagaaatgga atgggcgtat catttattcc aagaagcgtg cctgattgat ttaggaccag
    11461 atccgcattc atcagatgac ggaattcatg ctgcttcatt aggagcacta tggctagcag
    11521 ttatatttgg tttttcaggt attacagtaa cgaatggaac tctttctatt tctccaagac
    11581 ttccttcagc atgggaaaaa atttcttttc cttttatttg gaaaaatcac aggttgctat
    11641 ttgttttgac aaaaaaacaa atagtaataa aaaaagagac gaaagaaaca ttacctattt
    11701 ttgtcaatgg agaccaatac cagctggaaa aagaactgat cttggaacgg aggggaaaat
    11761 aatgcgtatt ctgattgcgc cagattcttt caaagaaagt ctgacagcct tagaggcagc
    11821 acaagccatt caatcaggtt ttcaagaagt ttttgttcag gctgaatatg atctgctacc
    11881 tataggcgat ggaggagaag gaacggtttc atcattagca gacagtttaa ggttgaagcg
    11941 gcaaacagca gtcgttacta atgcatttgg caaaaacaaa gaaatccagt acgcttcaga
    12001 cggtcaaact gcgatttttg aaatggcaga aatcgtcgga ttgaaagatg tcccactaaa
    12061 ccaacaacag ccacttaggt tatccacgcg aggagtagga gaactgatac gacaattgtt
    12121 gcaacaaggt gttcgacaca tcatcatcgg cgttggcgga agtagtacca acgatggcgg
    12181 catcggcatg gcagaaggtc taggttatgc tttttacgat caagataatc aaaaagtaga
    12241 agcactggga gaaaatctag gtaaaattgc tacagttgat aaaacgaacg tgcctattga
    12301 gctgattaaa gccaaaatca ctgtcattac tgatgttact aatcttttat gcggaaaaga
    12361 aggcgcttct tttgttttcg gaccacaaaa aggcttgaaa gaaaatgaat tagcagtggc
    12421 tgaccaagcc atgcatgatt tttatatgag atttgcccct caagtaatcg aggcatcggg
    12481 cagtagtgct ggtggtggaa tgggtgcagg actgttagca tttgcttcag ggaaaatagt
    12541 ctctggcatc gactacgtat tggatttact ggagttcgat aaacgggtac agcaagcaga
    12601 cctcgtaatc gtaggcgaag gacgtctaga tggacaaagt ctaaaaggaa aagcgccagt
    12661 tgggattgct agaagagtac cagagggcgt tcctgtgata gctatttgcg gaagtgttgg
    12721 taaaggaagc gaaaaagcag cagatgttgg catttcggcc gtttttccga tccttcctgc
    12781 agctgtgcct ttacaagaag ctttacagca agccactgat aatcttcatc gcacagctaa
    12841 aaacatcgca tctttacttc aagtcgttca ttttcaagga ggtaatcgat gaaaatcaaa
    12901 gcagcactat tcgatttaga tggcgttctt gtagacacag ctcgttatca ttacgaagcg
    12961 tggctggttt tagctaacca gctgtccatt ccattcactg agaaagaaaa cgagcaactg
    13021 aaaggaatat ccagaaccga gtcactagag cgattgctgt catttggaaa aatggaacag
    13081 aaattctcag aaaaagagaa atcagccttt gctgagcaaa aaaacaatct atatcttcaa
    13141 gcgattcaaa aaatggatga aacatccgtt ttgcctggtg caatagctgt tcttgagtac
    13201 ttgaaaaaaa caaacatcaa gatcggattg ggatcagcaa gcaaaaatgc acgcctcatt
    13261 ttagaaaaaa cgaatttgac aagttatttt gatgtcctta ttgacggcac gcaagtatca
    13321 aaagcaaaac ctgatccaga agtttttctc aaaggagcac agcaattgaa tgtaccgcca
    13381 aatgcatgct tggtcattga agattcagaa gctggttgtc aagcagcttt ggctggtaac
    13441 atgcatgtat tgggaatagg tgaaaacata aatcttcctt ctgctgaata tgtgattcct
    13501 gatttaaccg tttttgatca agtacgttcg ttttggcatt tatcagaagc cgtatcacgt
    13561 atataatagt ttccgttttt tctgtcacaa gcaaaacgtt atcctccttt tggaaatcaa
    13621 ggtatgatga gaaaaaaggg ggattttata tgacaaaaaa agcagttttc gcaggcggat
    13681 gcttttggtg tatggtcaaa ccatttgaag agcagccagg gatcatttct gtgacatccg
    13741 gttatactgg cgggcatgta ccaaatccaa cttatgaaca agtaacaaca ggcacaacag
    13801 ggcatacaga agcagtagag atcgaatatg atccagaagt tatttcttat gatcagttgg
    13861 tggaaatcta ttggcagcaa accgatccga ctgacgcatt aggacagttc gctgatcgcg
    13921 gtgattctta tcgtccagtg atctactata atgatgaaga agaacgaaat attgcagagc
    13981 gttcaaaaca agcattgcaa gacagcgggc gattcgataa gccaatcgtg acaaaaatcg
    14041 aaccaagaag cgaattttat cctgcagaag attaccatca ggattattac aagaaaaaca
    14101 agctccatta caatctttat cgagaaggtt ctggacgtgc aggtttcctt cgtcgacatt
    14161 ggggcgtcaa gaaataagaa gccgaaaaag ctacttatgg aacaccgtat atcagaggtt
    14221 gtaaaaccag cattttgagc taatactgag ggtcagctga ttgaacacca tttagtggag
    14281 aggttgtgaa cctgtcgcct agctccaagt cacaaggatt tggtaagaga acactatata
    14341 tcagaggttg tgacaagtcg tttgtcacaa ccttttttca atacctaaga tgaaggacaa
    14401 tcagcaacgc actgctcaaa aaaggaatga aaggcagctt ttccaacttt tttttatgac
    14461 agataaagta aacagaaaaa acggatattc caaacagact agcaagaaac aagatctgaa
    14521 gcaactcttc tagagacaag ccaccactcc aagaaagcat caacagagta tcgccagctc
    14581 cgagatagga ttgaccatag cgaaggcaca caccgcctac gataaacgtt aacagcacac
    14641 tttgccattg gaagacgccg gcctgcaacc aaaaaatcca taaaattgcc caagaacaga
    14701 atagcgtgtg agaatggacc atgaagtaaa ataaatccat caacgaaaat agcaaagccg
    14761 aaagcatcca tatgaacgtc gaaaaatttc ttgcggaaga aaaagtaaag aaccaggcaa
    14821 aaagaccacc acataaggct tctgccaaca aatagtagaa gggcaaccga cacctgcaat
    14881 agcggcagcg aaatcgttgg acgatgatcg agagaatcgg aatcaattca taccaagaaa
    14941 gcgaacgaca gcatttgaca caatgagatc taggataaat aatagagtga cctaacggaa
    15001 tccgctgggc ggttaaacat aaaaatgatc cgatacagca accaatgata aaataaagta
    15061 acacgccatc tccccctttt caaaaccaag atacgcaagc ttaaactaaa tttttgtaca
    15121 tacataaaat ttgaggtgaa aaaatgaacg tttcaatcgg attagatatc ggaaccacac
    15181 aaaccaaagc agttgctttc acagatgaag cccaagaagc agcatccgct tattttcgct
    15241 accctttgat ccaagaaaca caagggatgg cagaacaaga tcaggaattg atctttcaag
    15301 gtgtctgcac cgttataaaa gaagtaatta aacagttgcc ttcagacaca atcattcaat
    15361 ttgtctcctt ctcctctgcg atgcatggcc tgatattgtt ggatgaagag aaaacaccgt
    15421 tatctcgaat gatcacttgg gcagataacc gagcagcgga cgaagcagaa aaactgaaat
    15481 ataccgtgct tggcaaagaa ttttatcggc agactgggac gccgattcat ccaatgacac
    15541 cattgaacaa aatcaagtgg ctgcaaaaca gccaacctga attattcaaa aaaactgctt
    15601 attttgtcgg tatcaaagac tatatctttt atagattgtt tggcgaactg atcagcgact
    15661 acggaacagc ttcaggaact ggatattttg atatccatga attccaatgg gcagaagaag
    15721 tgcttgacta cttgaacatt cgaatagagc aattgccgca agtctttcct tccacctatc
    15781 aagtaacagg tctaccatca aaaacagcag cagaactagg tttgccaaat gatatatcat
    15841 ttgttttggg aggggcagac gggccgcttt ctaatctagg gttaggtgct tttggtgata
    15901 cgatcgccgc tttgaccgta ggaacgagcg gagcacttcg ttttattact actcagcctc
    15961 agttccatcc agaaatggag acgttttgtt atgttctgga tcagaaacat tgggtagtgg
    16021 gaggagcgac tagcaatggt gctggcatct ttgattgggc agggcataca tttttacagg
    16081 aagtcgttca atctgctaaa gaaaaaggag aaaatccata tgatgcactg ctttcggcta
    16141 tcgcttttgt gccagcgggt gccaatggtc tgatttttca tccctattta cttggtgagc
    16201 gagcaccgct ttgggacgca gatgcgtttg gcagttttat cggtttacag cggcaacata
    16261 cagaaaaaga aatgatgaag gcgattctag aagggatctg tctcaacctt tatcggattt
    16321 taaaaggagt ttgccaggaa aatcagctca cagagatccg tgctacaggc gggtttgctc
    16381 aatctccgat ttttcgtcag atgatggctg atgtactggg ttacccgctt gttttcccta
    16441 ctgtcacaga agcatccgca ttaggagctg tgattttagg ctggagaagc atcggaaaga
    16501 tccaaagtat cagtgaagca caggaattga tagaattgaa agagcaagtt ggagtacaag
    16561 aaactgatca taaaaaatat cagaaaatct atccattatt tgtttcgaca caaaagcatt
    16621 tagcacagac ttatcagcaa tttgctcaac tgcgagaaga attagctgat tctgaaaaat
    16681 gattggcttt acggataaaa aagcgaactg aaaatcgttc tcaggtaaat gattttgttg
    16741 tacttttcat tgaaagtcta ctattatttt aagtgtaaga aaatgttagg aggcaagaaa
    16801 gatgaaattc caacaaacca aagaagtatt aaatcaatta gttgcagatc taagccaaat
    16861 gtctgttgtt gttcaccaaa cacattggta catgagagga ccaggtttcc ttacattgca
    16921 cccaatgatg gatgagttca tggatgattt gaacgctcag ttagatgaag tatccgaacg
    16981 cttgatcaca ttagacggtt caccttattc tacattgcgt gaatttgcag ataacacaaa
    17041 aatccctgat gaaattggcc gctgggatcg tactattcca gaacgtttgg aaacacttgt
    17101 tgcaggctat cgttatctag cagatcttta ccaaaaagga atcgaagttt ctggagaaga
    17161 aggcgacgat ccaacacaag atatctttat cgaattcaaa acagccacag aaaaacgtat
    17221 ctggatgatc caagctcatt tgggcaaagc gccagagatc gacgcgtaaa aactgttgtt
    17281 gaacttgacc gaaaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    17341 acgtcaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    17401 tgaacaccgt ttaccaagag gttgtaagcc tgccgttcag ctccgagtca caagaaacaa
    17461 ttttaaaaaa cagcttctta tgttttatgg atattgtgac ttgtgaccga ggagttggca
    17521 ggagaacacc gtttattcaa aataaggggt tgtgacaaga cttttgtcac agcctctttt
    17581 tgtttttcat tttgacagga aacgaccctt ttatataaaa gaaagaaaag aagtaaagtt
    17641 tcatataaat ttttcaatca agcagaaaaa cgttgacatt gactagatta gcgggtaata
    17701 tgttaaatgg tattgtttgc cccacaaaga gccccggaaa ctcgaagtgt atgtcaaaca
    17761 tcagaactta aattggagga aaataacgtg cgtacaacat atatggccaa accaggcgaa
    17821 gtagaacgta aatggtatgt agtagacgca actgatgttc cattgggacg tctttcaaca
    17881 gttgttgcat ctgtactacg tggtaaaaat aaaccaactt tcacacctca tttggatact
    17941 ggcgatttcg tcatcgtcat caatgcagac aaagtgaaat taacaggtaa aaaagcgact
    18001 gacaaaattt attaccgtca cagcaactac ccaggaggat tgaaatcaat cactgctggc
    18061 gaattgcgtg ctaaaaattc tcgtcgtttg atcgaaactt ctgtaaaagg tatgcttcct
    18121 aaaaacactt taggccgcaa acaatttaca aaattgaacg tatacggtgg agcagaacat
    18181 ccacatgcag cacaacaacc agaagtttta gacatcacaa acttaatcta ataggaggga
    18241 atttcattgg cacaagttca atatatcggc acaggccgtc gtaaaaacgc agttgctcgc
    18301 gtacgtctag taccaggaac tggcaaaatt actgtaaata aaaaagatgt tgaagaatac
    18361 attccacatg ctgacttgcg tgaagtaatc aatcaacctt tcgctgttac tgaaacaaaa
    18421 ggcgcatatg acgtatttgt taacgtaaac ggcggaggat acgctggaca atcaggagct
    18481 atccgtcatg gtatcgctcg tgcattgcta gaagttgatc ctgacttccg tttagctctt
    18541 aaacgtgctg gcttacttac tcgtgacgca cgtatggtcg aacgtaaaaa accaggtctt
    18601 aagaaagcac gtaaagcttc acaattctca aaacgttaat atcctttata tatcaacgtt
    18661 tctggcactt ttcaattatt tggaaagtgc ttcttttgtt tttaagaaca catttaagaa
    18721 cacatatcaa tcacacttat tcaatcctct ctgaaaagct tcaaaaacag ccttatcaac
    18781 tatatctggt gtgttcacat atatctttgt tgtagaaata tctgagtgtg ttaaagcatt
    18841 tgaaatttga ttcatatctg ctccaccttg tctagctata gttgcataag tatgacggag
    18901 tttgtgtgga gataaatgaa ctaagtcagg atgccttctt ttaattgaat tgattcgata
    18961 gtttaggtaa tcggcatgaa caggacaatt tacttctcca gatggtttag tataagtaaa
    19021 gagatactga tctaacgttt gcttaatttt taatttcaac aattgatcag cttgtaccga
    19081 tttccattca cttagcaatg tcattacaac ttctggtact tgaatgacgg tatcttttcg
    19141 accttttgta aacttttttc tttgatattt atctaaagta tgtttaagac ggaccgtttg
    19201 tttttcaaaa tcaatatatt tccattgcaa agcgtaagtc tcactttttc tatcaccaag
    19261 atacagggta atcataaaaa gtgtgtaatc atgaaaagta agtaaatgat tattaaaatc
    19321 atcatttact gcttctatcc aattagtaag ttcttttgat gatagtgctt gcttaccttc
    19381 ttgtattctt ttttcttcta aagcattttt tttaggtgca gtgatacttt gaataatttt
    19441 agtagttcga ttaaattcga tataatttag tatttctgca atatcgaaca tggaacgaac
    19501 atatcctttt atgattttta tatttgcata ttctttcgat tttttggtta attcatctga
    19561 tattatctca gtattgatat ttagataatt cattgcgtat tcaccaaaca taggtaaaat
    19621 atgcaaccga aatatatctt ttgtattact aatggttata tcactaggag gcctgtttga
    19681 tctaattgtt tgacctaatt catatctagg aagccattta gattcgtaaa attttttaaa
    19741 cgttattttt ccttttaatt ctaatgagtt tgcattctcc tctctcaaaa ctttttctat
    19801 tttcttcttc atatcatttt cggctttaat cgccaattgc ctatttggaa atgttttttg
    19861 atacttttct gatgcaattc ctgttatttc tttaaacctt tttggatatc tcagattagt
    19921 tttatatttc ccatttgaaa gctttgtagg caaaatcctc actccttaaa aaaagtaagg
    19981 cagtatattt cactaatttt agtatatgcg gcgagaagaa aatttcaagt tattttaaat
    20041 gtattcataa agtctttaat atcttctacg tcaaagcgaa ctacaccacc aactcttatt
    20101 actgggaaat tatatatacg aatccactta tctagagtat tattatcgat tcctagaaat
    20161 gcacaggttt gttttttatt caggtagact ctggattcta taaaatcatc tcctaaaata
    20221 gtagttatga aatttttgaa ttgctttggg taagcacata ggaatttcac ctctccccag
    20281 tttacggcga gccttacgga agtatcatta tttgctgcta ttgtcatggc attttgtttt
    20341 cccagaacaa aaagaaatct ctaagtattt gtcgctaatc aggtcatggc gtacttgagt
    20401 ttctagctcg aatagcaggg atcgttattc aaaaattgta tttaattgtc aatgatcaaa
    20461 aatagttttt ttcctattca ctattaataa cgataaacat gtataaaaag acacactacg
    20521 aaaaaagtta aatggagttt actgattttc atatttgtat atggaacaga catagtcttg
    20581 aataattttt ataaatgttt tgatgcttat caacttattc aacccgctat ccacttcaag
    20641 tataattttt ccatacgcca ataaagtagt tttaggagca tgttctcatt tgagaacgct
    20701 ccttttttat atattttgaa agctacttac tatacgcgta gtttcaaata gttttgtcat
    20761 tctaatcttc agcaacagaa aaaaatctaa actataaaat ttagggttga ctaaaatata
    20821 atttttgtat accatatgat cgtgataatc attatcattt agaaggagaa agttatgtta
    20881 gaagtgaaaa agcttaatgt ttgttataac gattttttag cattgaacga gatttcttta
    20941 aaaatacaag aaggtgcttt aacaggtatt attggtccaa atggtgctgg aaaatccaca
    21001 ttgcttaaag caatgttaaa tattatccct catcagggag aggtctccat taataatgaa
    21061 gatatcaata aaaaactggt gaagattgca tatgtagagc aaaaagctga tattgatttt
    21121 acttttccaa taaaagtaaa agaatgtgta tctatgggaa catatgccgg aatgaaagtt
    21181 tttcaaagaa ttaaaaatgc agaatggcaa agagtttcaa aggctttgga aaaggtcgat
    21241 atgggaaaat attctaatca tcaaatagga gaattatctg gaggacaatt tcaacgtgta
    21301 cttttagcaa gatgtcttgc tcaaaatgcg gatttcatat ttttagatga accatttgta
    21361 gggatagact tagttagtga acgaataatt atggatacgt taaaaaaatt gaaacatcag
    21421 ggaaaaacag tgctaattgt gcatcatgat ttaagcaagg taaagaagta ttttgataat
    21481 attattcttt tgaatcgaaa tctaattgct cacggaagtg tagaatctgt atttaatgaa
    21541 gaaaatttaa aaaaagccta tggagatact atatttattg gagaggggga ataatatgct
    21601 tcaaaatttt attaatggtc tgtatgactt tcacttttta cagaatgctc taattacatc
    21661 tgtagttata gggattgttt ctggaacagt aggttgtttt attattttaa gaggaatgtc
    21721 tcttatgggt gatgctattt ctcatgctgt tttgccagga gtagcaattt cttatatttt
    21781 aggtattaat ttttttattg gtgcgattgt atttggtcta ttagcttcta tcattattac
    21841 ttttataaaa agca
//
LOCUS       DACCXM010000045        20325 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCXM010000045 DACCXM010000000
VERSION     DACCXM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.047"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(42..1181)
                     /locus_tag="IVY61_001381"
     CDS             complement(42..1181)
                     /locus_tag="IVY61_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP7970503.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1580..2878)
                     /gene="eno"
                     /locus_tag="IVY61_001382"
     CDS             complement(1580..2878)
                     /gene="eno"
                     /locus_tag="IVY61_001382"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP7970504.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2994..3749)
                     /locus_tag="IVY61_001383"
     CDS             complement(2994..3749)
                     /locus_tag="IVY61_001383"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP7970505.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3853..5043)
                     /locus_tag="IVY61_001384"
     CDS             complement(3853..5043)
                     /locus_tag="IVY61_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP7970506.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5153..6154)
                     /gene="gap"
                     /locus_tag="IVY61_001385"
     CDS             complement(5153..6154)
                     /gene="gap"
                     /locus_tag="IVY61_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7970507.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6195..7232)
                     /locus_tag="IVY61_001386"
     CDS             complement(6195..7232)
                     /locus_tag="IVY61_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP7970508.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            complement(7435..8769)
                     /gene="rpoN"
                     /locus_tag="IVY61_001387"
     CDS             complement(7435..8769)
                     /gene="rpoN"
                     /locus_tag="IVY61_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP7970509.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8829..8900
                     /locus_tag="IVY61_001388"
     tRNA            8829..8900
                     /locus_tag="IVY61_001388"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8862..8864,aa:Arg,seq:ccg)
     gene            complement(9092..9976)
                     /locus_tag="IVY61_001389"
     CDS             complement(9092..9976)
                     /locus_tag="IVY61_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7970510.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(10063..10962)
                     /locus_tag="IVY61_001390"
     CDS             complement(10063..10962)
                     /locus_tag="IVY61_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP7970511.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(10979..11485)
                     /locus_tag="IVY61_001391"
     CDS             complement(10979..11485)
                     /locus_tag="IVY61_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP7970512.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(11674..12294)
                     /locus_tag="IVY61_001392"
     CDS             complement(11674..12294)
                     /locus_tag="IVY61_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP7970513.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(12370..12807)
                     /locus_tag="IVY61_001393"
     CDS             complement(12370..12807)
                     /locus_tag="IVY61_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP7970514.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            12970..13800
                     /locus_tag="IVY61_001394"
     CDS             12970..13800
                     /locus_tag="IVY61_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP7970515.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            13784..15151
                     /locus_tag="IVY61_001395"
     CDS             13784..15151
                     /locus_tag="IVY61_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7970516.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            15170..15979
                     /gene="yidA"
                     /locus_tag="IVY61_001396"
     CDS             15170..15979
                     /gene="yidA"
                     /locus_tag="IVY61_001396"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP7970517.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16022..16465)
                     /locus_tag="IVY61_001397"
     CDS             complement(16022..16465)
                     /locus_tag="IVY61_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7970518.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            16599..16970
                     /locus_tag="IVY61_001398"
     CDS             16599..16970
                     /locus_tag="IVY61_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7970519.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(17139..17921)
                     /locus_tag="IVY61_001399"
     CDS             complement(17139..17921)
                     /locus_tag="IVY61_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP7970520.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(17918..18889)
                     /locus_tag="IVY61_001400"
     CDS             complement(17918..18889)
                     /locus_tag="IVY61_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7970521.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            18981..>20325
                     /locus_tag="IVY61_001401"
     CDS             18981..>20325
                     /locus_tag="IVY61_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7970522.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEIL"
BASE COUNT         5684 a         4202 c         3628 g         6811 t
ORIGIN      
        1 aatgctaatc tggctctgtt ttttcattta ttaaaaaaat cttacgctaa gtaggataaa
       61 ataattcctt gatatttttc gatcatatcc aaataattat cgatttcaac atactcatct
      121 acttgatgag gcaaagtaac gacaccaggc ccaaagacga caaagtcgaa ggaatggtta
      181 gctttcgtaa attcggctgc atccgttgtt gctgccgctc caactaaagg taatggctgg
      241 ctgaattgtt gctgaatgca atgaatcaaa ggagaatctg gatcagcttt gacaggtatt
      301 ttgttgtaat cgatcatcaa ttccaaatga tagtctgtct cttgattcaa ttcattgacg
      361 attgattgca gcaatgcaat gattttatca tttgggtatt ctggaatcga tcgaatgttc
      421 ccttggagct gcgcatggct tgggatgctg ttgacttgat tcccgccact gatcaatgta
      481 acattatgga ttgtttttcc taggacagga ttctcgattg tttcagcgag gtgattcatt
      541 tcagcatttg ctttcgtaat gaattcattt aggtgattga ttgcattata accttggtct
      601 ggcatagagc tatgcgcttc ttttccgtgc gaggtgactg tatagttgat tgatcccatg
      661 tgcgtataca tcaaactgta attggttggc tctccgatga tcaaggcatc taagtcatct
      721 acatagcctg ctttcgtgag ctgttcccca cctagttcac ccacttcttc tccgacagtg
      781 gctaataatt tgactgttcc gttaaatggt tttcctgact ctttcagttc gatcatggca
      841 atcaccattg ctgccaagcc actcttcata tctgtagcac cacgtccgta caaacggttc
      901 ccatggattt ctgctgcaaa gggtgcatag gtccaagacg attcatcacc agccgccacg
      961 acatccatat gcccagatag gcctaacact tttcctgatt ggcccttttg gtaacgagca
     1021 atcaaattat ctctaccatc tctgtaagaa acaatctctc ctgttatgtc atgctttgcc
     1081 aaaagcttat tcaaataggc tgctacttca ccttcgtttc cattcactga tttaattcgg
     1141 atgatctctt gtaaaatcgc gattttttct tcttttttca tctaatcctc tcccttgcca
     1201 tttcttttta ttgtatttga gtgtgctcga attttcaaat aggagcaacc aaagtaaaaa
     1261 tgaagaaaga gagccacttt atgaaaaata tgctgctact tccctcctat tttatttata
     1321 cgagaggact tgctcataga ttcctattct actgttctat ccttaaaagc tcttatatga
     1381 tgctagttat tgttagcttt cctttttcaa ggatgatctt ttcttgtacc attctttgta
     1441 aacatcatat acagaagcta gttgcaaaaa aactctctac ctgctgatat ttcagcaagt
     1501 agagagttcg attacttaac taagtgatca tactcgacta tctttcaata gtcgatcatg
     1561 tatcttcatt agaaaagaat tatttgtttt ttaagttgta gaaagatttc aaacctttgt
     1621 attctgcaac ttcaccaagt tggtcttcga tacgtagtaa ttggttgtat ttagcaatac
     1681 ggtcagtacg tgatagagaa ccagttttga tttggccagc gtttgttgca acagcgatat
     1741 cagagattgt tgaatcttct gtttcaccag aacggtgaga tacaactgct gtgtagccag
     1801 cttctttagc catttcgata gcttcgaaag tttctgttaa tgtaccgatt tggttcactt
     1861 tgataaggat tgagttagca attccttttt cgataccttc agataatttt tgagtgtttg
     1921 ttacgaacaa gtcgtcacca actaattgaa ctttgtcgcc taatacgtca gttaatttct
     1981 tgaatccgtc ccagtcgttt tcgtctaatc cgtcttcgat tgagatgatt ggatatttag
     2041 aaactaattc ttcgtagaat ttgatcattt cgtcagttgt tttttcgcct tcgcctgaat
     2101 cagctagtac gtaaacacct ttttctttgt cgtagaattc tgaagaagca gcatccatag
     2161 caagaacaac gtctttacca ggtacatagc cagctttttc gattgcttcg atgattactt
     2221 caaaaccttc ttcgtttgaa ccaaggtttg gagcaaatcc accttcgtca ccaacagaag
     2281 ttgctaatcc gcgagctttc aagattgaag ctaatgcgtg gaatacttca gcacccatac
     2341 gtagagcttc tttgaatgtt ggagcgccaa caggcatgat catgaattct tggaagtcga
     2401 ttgagttatc agcatgtgat ccgccgttga tgatgttcat cattggtgtt ggcaatactt
     2461 ttgtgttgaa tccgcctagg tagtggtata aaggtacttc taggtagtca gcagctgcac
     2521 gtgcaacagc gatagaaaca cctaaaatag cgttcgcacc taatttacct ttgttaggag
     2581 ttccgtctaa agcgatcata gctttgtcga tagccatttg gtcacgaaca tcgtagccaa
     2641 tgattgcttc agcaatgatg ttgtttacgt tgtcgacagc tttagttaca cctttaccgc
     2701 cataacgagc tttgtctccg tcacgtaatt caactgcttc gtattcacca gttgaagcac
     2761 ctgaaggaac cattccgcgg ccaaaagctc cgctttctgt gtatacttct acttcgattg
     2821 ttgggttacc acgtgagtct aagatctcgc gagcgtaaac atcagtaata attgacatgt
     2881 tttgtctctc ctttgagttt gttttttcct aagggatttc cctcatatac gattttagtt
     2941 aattttgctc attagtgcaa acatttcttg catttttcgc aaattttcaa caattatttt
     3001 acagcgtcta acaatgacaa gaatgaatct gcttgcaagc ttgcgccacc gactaaagca
     3061 ccgtcaacat tttcttttgc catgtattca gcaatatttt ctggttttac agaaccgccg
     3121 tattggatac gaactgcttc tgatacttct tttccataaa gtttttcaac tgtttgacgt
     3181 acaactccac aaatttcgtc agcgatttgt gcgtcagctg atttaccagt accgattgcc
     3241 cagattggtt cataagcaat aaccatgttt gacacctgtt cagctgaaag atcagctaaa
     3301 ccagctgtaa tttgtccttc gatccattca gcagttttgc ctgcttcata ggtttctaaa
     3361 gattcgccac agcataggat tggtgtcata ccatttgcaa agatagcttt cgctttttta
     3421 ttgatgtctt cgtctgtttc atggaaatat tcacgacgtt ctgaatgtcc gatgatcaca
     3481 taatgaacac ctagatcagc aagagctgct ggtgaagttt caccagtgaa cgcacctgag
     3541 ttttcccagt agcagttttg tgctgagatt tttagttctg taccttcagc tgatgtcatt
     3601 aattcttgta agaataatgc aggagatccg atgactgaat caactttgtc atttgaaggg
     3661 attttgtttt ttactgcttc agcaaattct tttgcttctg atgcagtttt gttcattttc
     3721 cagttacctg cgataatagg tttacgcatt gaggaacaca ctccttaatt tattgaaaaa
     3781 tggctaggga gtcatgaaaa ctcccttcac cttttgttcg attactcgtc agaattgagc
     3841 aaacttggtc gtttatttat cgttgattgc tgctagtcct ggtaattctt taccttcaag
     3901 caattccaag cttgcgccgc cacctgttga gatgtgtgtg aatttgtcag caaatcctaa
     3961 ttgttctgct gctgcggctg agtctccgcc accgatgatt gttgttgcgc cttctaagtt
     4021 agcgattgct tcacaaacac cgattgtacc tttagcaaag ttactcattt caaatacacc
     4081 cataggtccg ttccaaacaa ctgtttttgc accttgtaat gtatcagcaa ataattttac
     4141 tgatgctggg ccgatgtcta aagccatcaa accatcagga atgtctcctt cgattacttg
     4201 tgtttcaaca tcatttgaaa attctggtgc acagacattg tcgattggca agacaagttt
     4261 gtcgcctgct ttttcgatta attcttttgc tagttccact ttatcagctt ctactagaga
     4321 gttaccgatc ttgattcctt ttgcttcgta gaatgtgtaa gtcattccgc caccgatcaa
     4381 gattttatct gctttaggaa tcaagttttc gattacgccg attttatcag aaacttttgc
     4441 gccaccaagg attgcaatca ttggtcgttt tggttcttct actgcttcac cgatgaactt
     4501 gatttctttt tccatcaaga atccagcaac tgttgggata ccagttgaag cgatacctac
     4561 gttagaagca tgtgcacggt gagctgtacc gaaagcatcg ttaacaaata catcgcctaa
     4621 tgaagcccag tatttaccta gttctgtatc atttttgctt tcttttttac cgtcaatatc
     4681 ttcaaatcgt gtattttcga atacgacaac gtcgccgtca ttcatgttgc ggattgcttc
     4741 ttctaattgt tctccgcgag tttcaggtac gaatgttact tctttaccta ataattcgcc
     4801 tagacgtttt gcaactggag ctaaagattt tccttcttta tcttcttctg tttttacacg
     4861 tccaaggtga gagaaaagga ttgcttttcc gccttgttct agaacgtagt tgattgttgg
     4921 caatgctgct ttgatacgag tgtcgtcagt gatcacgccg tctttcaaag gaacgttgaa
     4981 gtcaacacgg acaagaactt ttttgtcttt taaatcgata tctttcactg ttttcttagc
     5041 cattttattt cctcctgaat aaacttatta tcaaaaaaag cggaagaagc gcgattgctt
     5101 ccccgctttt aaatgtacat gtctataatt tacacttgta cgtttgtcta tcttataagt
     5161 tagcgaagta ttctaaagta cgtactaatt gtgcagtgta tgacatttcg ttgtcgtacc
     5221 aagcaacagt tttaactaat tgtttgtcgc caactgtcat tactttagtt tgtgtagcat
     5281 caaataatga accgtaagtc ataccaacga tatctgaaga aacgatttcg tctgtgttgt
     5341 atccgtaaga ttcgtttgaa gcttctttca ttgctgcatt gatttcgtca acagttactt
     5401 ctttttcaag aactgttact aattctgtta atgaacctgt tggtacagga acacgttgag
     5461 cagctccgtc taatttaccg ttcaattcag gaattactaa accgatagct ttagcagcac
     5521 ctgttgagtt aggaacgatg tttgcagcag cagcgcgtgc acggcggaag tcacctttag
     5581 gatgaggtcc gtctagagtc atttggtcac ctgtgtaagc gtggatagtt gtcattaatc
     5641 cttcaacaac accaaatttg tcgttcaatg ttttagccat aggagctaaa cagttagtag
     5701 tacatgaagc acctgagata actgtttctt taccagttaa tgtttcgtgg tttgtgttat
     5761 aaacgattgt tggaacatcg tttccgccag gagcagagat aacaacgcgt ttagcaccag
     5821 ctgttaaatg tttttcagct gctgatttag aagtgaagaa accagtacat tctagaacga
     5881 tatctacgcc tagttcgccc catggtaatt cttctgggtt gcggttagct aatactttaa
     5941 cttcttttcc gtttacgttg aatgaacctt catgaacttc aactgttccg ttgaaacgtc
     6001 cttgagttgt gtcgtatttt aacaagtgtg ccaacatttt agcatctgtc aagtcgttga
     6061 ttgctactac ttcgattcca tctacatctt ggatacgacg gaatgctaga cgtccaatac
     6121 gtccaaaacc attaatacct actttaactg tcatttaaga tttcctcctt atgaaaatca
     6181 aaaaaatatt tattttaaag ggttacccct tttaaaatct catttgcagc agcttcatca
     6241 gtgatgagcc acgtttgttt tggtgcattt ttcatataag ctcgaattgc tttagctttt
     6301 gattttccgc ccgcgattgc catgatgatc gggacttttt ccaaatcttg caattctatg
     6361 ccgattctag ggaccttata aacgacttca cctttttcat tgaaaaagta gccaaaagat
     6421 tccgcaacag catttgcttg tttcagcatg actaattctt tttcagtcat cttacgacgt
     6481 gctgccatgt gtaatgcacg cccaatacta tgaataacac aattcgcttc actaatcaag
     6541 tttaagactt cttggataga tggctcattg agtaaagaat tgtaagttgc cagacttaat
     6601 tgttccggaa cataaagtgc acggtggtta ccgtttgctt tagcagccat tcttgcactg
     6661 acagaatttg cctgtacagt tatcgcttca ccaatcccgc ctcttgcagg gacaaacagg
     6721 ttatgccgtt tcttattttc cagattagac aattgttcgg ctaccacagc catagtagtt
     6781 cctcccatca cagcgatgat attctcgcct tctggaagta gtcggtctag ggcttcgctc
     6841 agcgcttcgc cgaatgcatc agccactttg gtctgctctt cgctgtttcc tgaaacaacg
     6901 atacagcgtt ggttaccaaa atattctgcc aactgttgtt cggtttgatg cgtacctaaa
     6961 agctgatcca tgaagttttc aaggctttga taaacttctt cgcccttagt tgtcagcgtc
     7021 ataccgcttt ttgaggtgtc gatcaaattc agatttttca aaagatctgt ttctgttcgc
     7081 aatactcgtt ctgtgatccc catactttcc gataaacttc gacgtccaat cggctgcatc
     7141 caatagatat ttcgtaaaat tttatagcgt tcttgcaaca cttcgataat atctggtgca
     7201 acagattcaa tcatttttat ttcatccagc atagcgatct ccttagttgg acactttacg
     7261 tccaatgtag acttaaaacg acccatatac gataaaaaaa gaaattaact attcgaattt
     7321 cacatcaata aagggaaatc tcaacctcac gactgtaagt ctaacagttt tgctctcgtt
     7381 tttcaactaa aaagactaga aaaagtgaaa tactttttct agtcttttga ctgattagtt
     7441 atcataacgt tttctttttg atgaactcgg gattcctaat aaatcacgat attttgctac
     7501 tgttcgtctt gagatcgcta tctcatcttt tttcagcaat tcaaccaatt tctggtcaga
     7561 caacggcttg tttttgtctt cttgatcgac taattcctgc agctttttct tcgctgtatc
     7621 tgctgaaagg tcttctgtct gttctgtact attcgtagga attcgtgtag tgaaaaattt
     7681 ttttaattca aatacaccga agtctgtttc caaatatttc ccgttgactg cacgactgac
     7741 cgtggattca tgcacatcta tctcagttgc aacttccttt aacgtaagag gcttgattgg
     7801 acgttccttg tcgataaaga aagctttttg ccgggaaaca atgacttgag caacccgcaa
     7861 aattgtatcc ccacgttgta ggatcgtttt cttcaaccat tcgaattcct gtttccgttc
     7921 ttttaaataa ttttcagttt ctttatctgc ttgttgtgac atttgtttga agtaactctc
     7981 ttgaaagcgt atatttggct gattttttcg gtttgagata acttgtacat ttttatcgtc
     8041 gtccactaat acccggacat caggaataat gtacaattcg ctgctacggt cgaaaatccg
     8101 gccagggctt ggactcaacg tttgtaaata gtcgaatatc tgctgaacag catgtagatc
     8161 aacagaaaaa cgttgtgcga tttctttcca ttttcgctcc accagttcct caaaacattc
     8221 ttccaacacg atataagcta attccggcgc ataatcatct cgttctgttt gaagcatgag
     8281 acattcttgc aaactacgtg cacctactcc agctggttct aattgctgaa tcagagtcaa
     8341 cgcatcgagc atttggatag gcgtagctcc aatctctttt ccggcttctt ctaaactaat
     8401 cgtcaaaaat ccgttcaaat cgatgtattc tacaagataa agaacgattt tcctcaaaaa
     8461 agtatctcgg taattcaaat ggacttgttc gatcaatgaa tcaaataaag acccttttgt
     8521 atcaggaatc tgatttaggt aatttgtctc ttctccttca taagaagatg aagaagcttt
     8581 tatgtaatct ggctgccatt ccggctcaac aacttccacc aatggatttt ccattgcttg
     8641 attctcaacg aatgccaaca gctcctcact gttatattgt aaaacttgga tggactgttg
     8701 caactgttgc gtcatggcta gtttctgtgt ctgctgctgt ttttgtgaaa aattttgctc
     8761 aaatttcatt tttttgtcca atccccttgt aattttttat ctgtttagtt acaattatcc
     8821 cagtgcttgc gtccttagtg tagtggatat cacgtaagat tccggttctt gagacggggg
     8881 ttcgattccc tcaggacgcg ttctactgct attttagcat aatttacgaa tagttgcttc
     8941 tttattttta agtgaaaact gtccgccaaa gaaaattggt ggacagtttt ttgttgtacc
     9001 tgtatagata ttgttaagcc tttgcaagta aggtagttat gtgttcaact acttattaaa
     9061 atgtgttatt tattaaagat acttgtctca cttactgaac tggccgatga tcgtttctgc
     9121 cataatagtt ctcgacagcc gttggatgcc ctaataaatc cccaactgtc gtgatgcgat
     9181 acccttgctc tttcagataa tcgatcactt gaggaacggc acgaatcgtt tcaggataga
     9241 tatcatggaa taaaataatt gccccatcgt atactgttgc ttggatcttc tgcaaaatca
     9301 aatcaggatt tcttgtcttc caatcttctg aatcaacaga ccattcgatg atcggtcgct
     9361 ggatgatcgt tgctcctaca ttcgtgacag aaccataggg cggacgaaca aaagtggggg
     9421 ttttgccagt gacttttttg atctcagtgt ctgctttcgt cacttcctcc gtcatcgact
     9481 gggcagaaag agtggccaga ttctgatgat cgtatgtgtg attgccaatc tcgtgccctg
     9541 cttcagctgt ctgcttcacg atttcaggat gctgcgccgt attttctcct aagacgaaaa
     9601 aagtggcatg cacgtttttt tcttttaaga ttcgcagaag ctgcggtgtt gtggtcgctc
     9661 ctggtccatc atcaaagctg attgcgacgc ttttgacagc cggatctcgg ctcgtttttg
     9721 tactgtcttg tgttgattgt gatgactgac tttgtacctt ttccgctgat tcttgaatgg
     9781 cttctttgac gttagctgtt tcagttgatt ggcttttaat ggttgattgt tctgattgtt
     9841 tgaaagattg tgacgaatgc tccgaagagt gtgtttgtcc ttttacttcg gtgatcaagg
     9901 aggccaccga agtaatttcg aaaaacaaaa gcacggataa agcaataata ctgataacta
     9961 aataatttct tttcactata tctcttcttt ctctttttct cttcttatta tttacttgta
    10021 cttacttgaa aattgaaact gctagtcgca actacttctc gtctaatgat ccaaataatc
    10081 ttccgatgcc aaaatcgttt ttgtcacgga cttaggaatg acaaattctg gtgtacccat
    10141 ataaccagga gcaatttcgt attgagaaaa agtcaaaact aattgatgct ctttgttcaa
    10201 ataaaaactt cgtgtaggcg taacaagtgt tggtttttct atcgtccctt cttgaatatc
    10261 ttgctcagtc caatagtact tagatggatc ttccttgact tgttcggcca tctgccgttt
    10321 gacttctttt gtcaaaacag atagataggt gtcatctttg aataaaagtg gaagtgagag
    10381 ctggacactg tttttcttgt cgattgtgtc gtatcttttt tcaacatggc tatctgctgc
    10441 tgtttgggtg atctgccttt caatcacgag aaaccggtta tcgtctacga ttttctgata
    10501 atctcccaaa acttggtaat gatccgtttc aaaatttcca tattcttttt cagctttttc
    10561 aaaatctgct tgtccttcgc gaaagtattt ttgatttaat ccatgaattg ttttattttc
    10621 tttctcattt tgtgtattga tcttcggtac ctgaatatcg attttttcag atcgatcagt
    10681 aaagcgttcg cctaagatca attcaatgac tgatcctaag atgggtaaat cttctgcaaa
    10741 gcttcgaact tgactattaa aaagaaggct acttgttgta atcattacga tagccaaaga
    10801 tactccgaac ccttggagac gtctctttct ccgctttgct tttataaatg attgctcttc
    10861 gataataaaa cgctggcgga tctcctcttt aagccctttg ggaattgctt gttctttata
    10921 agttttcttt agttttttta aattttcttt ttgggaatac atggcactct tctctccttc
    10981 attctaggat ttgacgtaaa gcattcaatg agcggtacat tttcgtttta actgtgtttt
    11041 cattcatatc caagatagtc gcaatttctt tcaaacgaaa tccttcaaaa aagtagagga
    11101 tcagaatctc tttttccgga ctgtctattt tatctaaaat catatggagc tctgcaacgg
    11161 aagtttctaa aggtaactgt tgggggatat cttgaatttc aaataagtcc acaggcgtcc
    11221 ttttttgtct gcgccaacta tctaatgccg tgcggatcaa tatccgataa aaccatgcac
    11281 taaaatgctc gatctctttt tctttttcaa aatagttcag ggctttatga aaactatctt
    11341 gcatcacatc taaggcctct tctttatcat gtacgtaatt catcgtgata cggaacattt
    11401 tttgataatc agtttcgatg agtccattca gcaggtgacg gtatttttcg atctgccgtt
    11461 ttttcttata tccagtaaaa atcattctcc actcctttct gcctcactta atatgacgta
    11521 caaggtgcgc taaaagtttc aattttttta ttttttatgt atttacttgg gatttttata
    11581 tacaaaaaag aaggtgtgac aaaaatcgtg tcacacctcc ttattctgat aggctgatcg
    11641 tctctgtcac aacctctcgt aagaaaataa tgtttattct ttttcagagt cagaaaattc
    11701 ttcctcttca taatcttcat catcatcgtc ttcgtctatg atacttaaat cttcttcgat
    11761 atttcctggc aagtcttctt catcatctgt cgggtcatct gctccgattt cttgcaagtc
    11821 agaattataa gcagcgattt cttcatcttc atcatcatcg tcatcaaaca gatcatcgtc
    11881 atcttcttct gtcagatctg tatcttctgg gtcatcgtca ttgtaatcga tcgcgtcttc
    11941 atcatcattg ataaaggcgt tgactttctt gcgtttacgg cgacgtggag tatcttcatc
    12001 ctcttcgtcc attccatggt ttacttcttc atcgatcgaa tcgatagcat accatgaacg
    12061 caagccccaa cgattatccc ctaatgagat aaagctgccg tcaatattca agtcagtata
    12121 aaattgtgcg agtgaatcac gaatttcact gtctgatttc ccaagatagt tttggatctg
    12181 gttagctaaa tcagaaaaat ccatgacatc gccacgttgt tctaagatcg cgtgtgctac
    12241 ttcgatcatt gataattcgc ttttgtttgc accttcaaat acactaattt ccaatcagga
    12301 cacgtccttt cacagtctac tccttatcat acaaaatcag acacaaaaaa tcaaactttt
    12361 cttttatatt tacgctgtaa aatccaaggt caaataatat tcgccgattt tttccggtcc
    12421 catgaacaga tcataatcga ttcgaacctt ccctgctgtg ggacggtctt tcaaactaac
    12481 atgcagttct ttcgtaaaag tagcaatctg gatcataccg tatggtgttc gataggatgt
    12541 ttctaaccgt tctttatagc caaatttcaa acgcatccgc atttctcctg cacggatcag
    12601 ctgtacatga ccatctggaa ctaatttgat cgttacagga atctcatcgc ccgtctcctc
    12661 ctgaatctct ttataacgta tgtacagcgt atcgccaatt gtcaccattt gccctttcaa
    12721 atcaaaataa aaatcttgca cttccccttg ctgttttact tttgtacgca atttgattga
    12781 aatcggtgtt ccttgtgata attccattat tcattctcct ttacttctca tagctacatt
    12841 gttcctagtt tcttttgttt tgtcaaccaa cttgtccatt tttacagaaa accttttttc
    12901 caaaacaact ctttgatttt agagtataat attgtagaaa atatgaattt atacaaagga
    12961 gccggttgaa tgaaccctgc gattcgacta gatcagcaca tttatcaaaa aaacgacttt
    13021 tccccttttg cattaacaga tatattaacg gaatattcta aagaacacca gcaaatcttt
    13081 ttacatttct ggcaatatga ctgcacagtc atcttaggta tgaaggatgt tcgaactcct
    13141 tttctaagtg acgggatcca aacactgaaa gatgcaggtt acgctccagt gatcagaaat
    13201 tcaggtggat tagggatcgt atcagatgaa ggaattttga atatttcttt gattttcccc
    13261 caaagtgaca caaaaaaaat aaccatagat gacgcttacg agcaaatgct cctattgact
    13321 aaatacgctt ttccaaaggc accgatacaa gcattcgaaa tcacccattc ttattgtcct
    13381 ggaacttatg atttaagcat ccatggcaaa aaatttgccg gtatcgctca gcgtcggatc
    13441 aaaaatggga tttctgttat gatgtactta agtgtcaacg gtaatcagga aaaacgcggg
    13501 caagtcatga agcgatttta tcaatctgct ttaaaagaag actttggtac aaatggctat
    13561 cctgcagtgg atcctgctac aatggcaaca cttgaagaac tgcttgacca accccttttt
    13621 gtaaaagaag tcaaagaagc atttgcaaaa gcttttgaaa agctgtattc tgcaagtgat
    13681 ttacaggatt ctaccgactg gctaaaaaaa gaaatccagc tggagcaatg gaacaaccaa
    13741 atccagagaa tggaacaacg taataacata aaggagctac attatgacaa ccccttataa
    13801 atatcaaatg ctgcctatgg aaaaagtttt tcgtgatcca gtccataact atatccatgt
    13861 ccagcatcaa gtaatcttag atttgatcaa ttcgaaagaa gtccaacgtc tacgacgcat
    13921 taaacaatta ggcacttctt ctttcacttt tcacggagca gaacatagcc gttttactca
    13981 ttcattaggc gtttatgaga tttcaagacg aatctgcgat atcttttctc gaaatttttc
    14041 aaaagaaaaa ataggcaacg gaggctggga tgatcaggaa cggctggtga ctttatgtgc
    14101 ggcactgttg catgatgtcg gtcatggtcc ttactctcat acatttgaac atatcttcca
    14161 tacgaatcat gaagccatca ctgtagagat catcacttct ccagaaacag aagtgtatca
    14221 gattcttaac cgagtagaag aaggttttcc tgaaaaggta gctagtgtga tccaaaaaac
    14281 ttaccccaac ccacaagttg tccaaatgat ttctagtcag atcgatgcag atcgaatgga
    14341 ctatttgctt cgggatgctt atttcactgg aacagaatat ggaacatttg atcttacccg
    14401 aatattacgt gtgattcgtc cttatgaagg cgggatcgct ttttccatga gtggcatgca
    14461 tgcagtggaa gattatatcg ttagccgata tcaaatgtat gtgcaagtgt acttccatcc
    14521 tgtttcccgt ggtatggaag taattttgga gcatctgctt catcgcgcac acgagttgta
    14581 tcaagaagat gccagcagtt ttgaactcca ttctcaatta cttctgccat ttcttgacgg
    14641 aagctttact ttagaagaat acctcaaact tgatgatggg gtcttgggca cttactttac
    14701 tcaatggtct gaatctgctg atcctatttt aaatgattta gctaaacgtt ttttgaatcg
    14761 taaaccactg aaatctgcta ctttttctgg caaccgggat tcaaaattag tccaagaact
    14821 cactttgtta gtagaaaagg tcggatataa tcctgtttat tacactgctg tgaattctag
    14881 ctacgatcta ccttatgatt tttatcgccc agaacaaggc agacatcgca cacaaatcga
    14941 gattttgcga aatgacggaa ctttgataga attatcacag gttagtcagc ttgttgctgc
    15001 attagcggga caagaacaag gagatgagcg atttttcttt ccaaaagaaa tggttgatcc
    15061 gtccttgcgt gatcattatg atttatttga cgaaacttat caagaatttg ccagccatat
    15121 ccgtaatggc gcattgattg aaataaatta aaatatattg gaggaaatta tgtctatcaa
    15181 actagtcgct atcgacatcg atggtacttt attaaatgag aaaagagaag taacaaaaga
    15241 agtaaaagaa gcgattgccg ctgctgttgc aaaaggtgtt tcgatcgttt tatgcactgg
    15301 ccgtccctta ccaggtgtcc aggaacagtt aaacgagttg aacctttttc aagataatga
    15361 ttacgttatt acttataacg gggcactcgt acaacaaacg aaatcaggta aaatcatcgc
    15421 ccgtcacgga ctgacacatg aagatttcct agagatcgaa gtgatggcac gccgtgtcgg
    15481 cagtcatctc cattcgatcg atgacaaagc gatctacacg cctaatcgag atatcagcgc
    15541 ttatacgatc cacgaagcaa gtttagtcaa gatgccgcta aaatatcgaa cacctgaaga
    15601 aatgacacca gacatgaaca tcgtcaaaat gatgatgatc gacgaaccag aaattttaga
    15661 tgcagcaatt gcgcgtttac cgcaaacttt ccgtgataaa tacacaacgg tcaaaagtgc
    15721 gccctattac ttcgaagtac taaataaaga agcaagtaaa ggtgcggcag ttgcgaatct
    15781 cgcacagcac cttggtattg atcaagatga aatcatggct atcggagata acgagaacga
    15841 cttatccatg attgaatatg ctggtctagg agtagcaatg ggaaatgccg tttctcttgt
    15901 caaagaagca gcaaatgtca tcacttcatc aaacgacgag cacggtgtag ctgaagcaat
    15961 caaaaaatat gtactgtaat aaataaaaaa actgagagaa agctgatcta ctcgctttct
    16021 ttcagttttt atttttttcg atccatctct cgactgccca tcggtggcta ccacgtttca
    16081 atttcacaag cgcttcttga gggctcaccc accaaatttt attggtcttt tcagttggtt
    16141 ctcctatacg cttccaagaa tcagcaacaa agaaatatcc tggattataa taataagttg
    16201 cacgataatt cgagtaaaaa tactcatcag cttctcccag ataagaagaa atttctgcca
    16261 caattcccat ttcttctatc agctcacgga caatcgcttc ttctttcgtc tcttctcctt
    16321 caatttcccc gccaggaaga aaaaaagcac catttggagc ctcgattact gcaatctgct
    16381 gatctctttc tatgacaata tatgctgcat aacgggtttg ataatcagca aattgctttt
    16441 tttcaccaaa aattggttta tccatgaata gtttccacct taacagtttt attttctatc
    16501 cattttacat gagaaatttc tcatgaacaa gcacaaatca actatttcct tacttctttt
    16561 tgttatgatt gagtaaaagg aaaaggagag atgaatctat gaaaatggct cacacatgtg
    16621 ttcgagtgaa agatcttgaa gcatcaatcg aattttatca aaaggcattt ggttttgaag
    16681 aaagccgccg ccgtgatttt ccagaaaaca aatttacact tgtttatttg acacttcctg
    16741 gggatgacta tgaattagag ttgacttaca actatgatca tggtgcgtat gatctaggag
    16801 acggctatgg tcatatcgct atagcagctg atgatctaga aaaattacac gagcaacata
    16861 aagctgctgg tcttgagatt actgacttaa aaggattacc tggcactgcg ccttcttact
    16921 actttgtcat tgaccctgat ggttataaaa tcgaagtcat ccgaggatag ttagtatggg
    16981 gcattttact cttgattgaa taaaaaacaa acaccaataa ttaaaatgag acgcaaactt
    17041 ttcattaaat tgaaaaagtt tgcgtctttt tttaacaacc attgtagcta taaggttgcc
    17101 actctccacc aatttatttt taattcattt gaaagaaatc aaataaacga ggtaccttta
    17161 gaaaacaaaa ctgcttttcc tcctattatc acacgatctt cttttaattc acattctaga
    17221 tccccgcccc ttgatgacat ttgataagct gacaattttg ttttattcat tctttctgcc
    17281 caataaggaa taagattaga atgtgctgat ccacatactg gatcttcatt gattgttaat
    17341 tttggaaaga aggttctaga aacaaagtct ttttgcacac tttctgctgt cacgatgact
    17401 cctattccta attcaaaatt tttgatagct gtaaaatctg gctgcaattt agccaccgtt
    17461 ttttcatctt ctaatacgaa aaataaatca cgtgctaagt aggcttcttt aatcttagcc
    17521 ccaatcgctt cttcatattc agacaatatc ggcacttttt tcggcataat actaggaaaa
    17581 tccatgtaat aataatcttt aattcttgtc acaaatagat taccgctttg agtagaaaat
    17641 ttaatcgttt catcaggtat tttgtaatag ttaaataaaa caaatgctgt tgctaatgtc
    17701 gcgtgaccac aaagatcgat ttctcgatct ggcgtaaacc atctaagctc aaattcttgg
    17761 tttttcttca ctgtaaatgc tgtttcagat aaattgtttt caatggcaat tttttgcatc
    17821 gtaccttctg gcagccattc ttctagcaca taaaccgctg ctggatttcc tttgaacacc
    17881 tcatctgcaa atgcatcaac tacgtaataa tctaagttca ttttaaagcc cctttctcta
    17941 atttgttata aatatattct gcacaacaaa gatcttcaat agctagacca actgcgtcga
    18001 aaatcgtcgg cctcgattct cctttataaa atgttttttt ctcagaaact aattcgccta
    18061 atgtaccaac aatacaagat tcctctaaat ctccttctgc catggctata agcaggtccc
    18121 cgctttctgt taaggcagct gagtaatcat ctacatagat ttgagaagtt tccatcaacg
    18181 atgtacttat ctctcttgta tcaggagtaa atgtgcccac agcattgata tgtgcgtctt
    18241 cccttaccca ctctttttga agaaatgcct ctttactaga ggtcaacgta caaatgattt
    18301 ccgctggacg tactgcatct tctactgata ttgcatttat aaacgttatt ttaggatact
    18361 gttttttagc tttagcgata aaagtatttc gtctttcttc taaaatatcg taaacaaaca
    18421 ctgtagtgat gggacgaatg gtagaaatcg cagtcaaatg cgacaatgct tgttgaccag
    18481 ctccaattaa agcaagtgtt tctgcatgcg gatttgctaa gtaatctgtt gccagcgcag
    18541 atacagcagc tgttcgaatc caggtgactg catttgcgtt tatcaatgct ttgggtagtc
    18601 cgttttgagc atcaaacaag aaaatctctc ctaaatgaga aggtaaatga tactgatgat
    18661 tatccggaaa agcggtaatg acttttgtgc caaaatatct gttctccccc aaataagcag
    18721 gcatcatcgc aaacatattt ttgccttttc cgtcaggtaa aatttccacc aatcgaggat
    18781 tttggctgat tttaccactt tcataatcaa aaaaacattt tttcattact tgaatcgctt
    18841 cttcaaaact aaaagaagct actacttcct caaaattaat aattttcata gacaattccc
    18901 tcctcacaaa agattaactt ctaaaatcaa tacaatcaat ggtaaaattg tatggtatac
    18961 aattttaagg agtggagaaa atgcctatta attcttatga caattacccg ttaacttggc
    19021 gaccagacaa acaaaaattg aagcgtcctt attatcgatc gttgattcaa caattagaac
    19081 aagatatttt aaagaataaa ttacagaaaa acactcagtt accctcccaa agagagctag
    19141 ctgattattt agatttaaat tttactaccg ttggcaaggc ttataaatat ggaattgaga
    19201 aaggtttgct gtatacgaat attggaagtg gcacatttat ttctcccaat gtctttgagt
    19261 ccatcaccat ctctaccaat gaagtaaccg aacatatcat tgattttggt ctggttagtt
    19321 cttttgaaga atgtaataaa ttcgttgtcc cgtttattca atcaactagt aataacccaa
    19381 atcttgttgg tttgcttaat taccaagatc ccaaaggcac aaattttcaa ttgactactg
    19441 cagcaaattg gttaaaaact caaggagtca ttccgtcctt agaatctcta tctattgttt
    19501 ccggtgttca aaatggattg gctatcacac tttctgcact tttctctcca ggtgatagaa
    19561 tagcaacaga ccgatataca tacgctaatt tcattgaatt ggctcagtta tatcatttag
    19621 agatcgttcc aattgatttt gacgagcaag gaatgcgacc tgatatattg attcaggaat
    19681 gtagaaaaaa gaagattcat ggtgtctttc ttatgccttc ttgtaataat cctatagggt
    19741 ttcaaatctc tcacaaaaga cgagagcaat tagccgaagt gattgaaaaa gaaggtctat
    19801 gggttattga agatgatatc cattcatttt taacaactta ctatcaacat acgatcattc
    19861 ctcctttcca aaatttatta cctaaccaaa caatctactt agctggaatg acaaaattta
    19921 tttgttctgg attgcgagtt gcttatcttg tagttccagt acatgtccaa agcaagatag
    19981 aacaagcaat ttttaatata aatgtgaaaa cttctggatt agatgtggaa gtcatcactc
    20041 aaatcctcca ttcaaatgaa gcaaatagga tcttagaaac taaattaagt cttacaaaaa
    20101 gagccaatca gctttttgat tctcttttca aagtagaaaa accaacgaat cgtgtccctt
    20161 actatcgaat tcttccagtt gatcctgaga ttgaccctaa gataattgag aatgactttc
    20221 ttgctaacgg tatccgactt tatcattcca gacgctttac tacacgtacg caaccagatg
    20281 cattcgttcg aatttcccta tcctctaata accttgaaat tttag
//
LOCUS       DACCXM010000046        19995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCXM010000046 DACCXM010000000
VERSION     DACCXM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.048"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            30..842
                     /locus_tag="IVY61_001402"
     CDS             30..842
                     /locus_tag="IVY61_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP7970523.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            839..1639
                     /locus_tag="IVY61_001403"
     CDS             839..1639
                     /locus_tag="IVY61_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP7970524.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1800..3068
                     /gene="rseP"
                     /locus_tag="IVY61_001404"
     CDS             1800..3068
                     /gene="rseP"
                     /locus_tag="IVY61_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP7970525.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3136..4845
                     /locus_tag="IVY61_001405"
     CDS             3136..4845
                     /locus_tag="IVY61_001405"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP7970526.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5057..9415
                     /locus_tag="IVY61_001406"
     CDS             5057..9415
                     /locus_tag="IVY61_001406"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP7970527.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9560..10033
                     /gene="rimP"
                     /locus_tag="IVY61_001407"
     CDS             9560..10033
                     /gene="rimP"
                     /locus_tag="IVY61_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP7970528.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10056..11231
                     /gene="nusA"
                     /locus_tag="IVY61_001408"
     CDS             10056..11231
                     /gene="nusA"
                     /locus_tag="IVY61_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP7970529.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11253..11546
                     /locus_tag="IVY61_001409"
     CDS             11253..11546
                     /locus_tag="IVY61_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP7970530.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11543..11854
                     /locus_tag="IVY61_001410"
     CDS             11543..11854
                     /locus_tag="IVY61_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP7970531.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11867..14173
                     /gene="infB"
                     /locus_tag="IVY61_001411"
     CDS             11867..14173
                     /gene="infB"
                     /locus_tag="IVY61_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP7970532.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14200..14547
                     /gene="rbfA"
                     /locus_tag="IVY61_001412"
     CDS             14200..14547
                     /gene="rbfA"
                     /locus_tag="IVY61_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP7970533.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14704..14877)
                     /locus_tag="IVY61_001413"
     CDS             complement(14704..14877)
                     /locus_tag="IVY61_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7970534.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15325..17687
                     /locus_tag="IVY61_001414"
                     /pseudo
     CDS             15325..17687
                     /locus_tag="IVY61_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18055..18978
                     /gene="truB"
                     /locus_tag="IVY61_001415"
     CDS             18055..18978
                     /gene="truB"
                     /locus_tag="IVY61_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP7970535.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18982..19923
                     /gene="ribF"
                     /locus_tag="IVY61_001416"
     CDS             18982..19923
                     /gene="ribF"
                     /locus_tag="IVY61_001416"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP7970536.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
BASE COUNT         6853 a         3519 c         4389 g         5234 t
ORIGIN      
        1 ggaatctttt aagtatagga ggaaaaagaa tgttacgttt ttttccgcaa aaaaacaaat
       61 atattcaaga aaaaagcgaa tttcaattta ataccgatgg accaattcct cagcacattg
      121 ccatcatcat ggacggcaat ggtcgttggg cgcaaaaccg tcgtttgcca cgagttgctg
      181 gccataaaga aggcatggaa acagtaaaga aagtcacaaa aaaagcatca aggcttggag
      241 tgaaagtgtt gacgctttat gccttttcga ctgaaaactg gaagcgacca aaagatgaag
      301 taagttttct catgcaacta cctgtcgact tctttgatac gttcgtacca gaattgatta
      361 aagaaaatgt caaagttcat gtcatgggtt atgaaaacgt attgccagaa catacgcaag
      421 atgctgtaag acgtgcaatt gagcaaacaa aaaacaatac tggaatggta ttgaattttg
      481 ctttgaatta tggaagcaga gcagagatcg tgactgctgt ccaagaaatt gccgaagaag
      541 tagcaaaagg agaaatacac gcagaagaaa tcgatgatga attgattgct aagcatttaa
      601 tgacaggttt cttgcctaaa gagttacaag atcctgagct gatgatccgt acaagtggag
      661 aagagcgaat tagtaatttt ctgttatggc agatcgcata tagtgagctg tattttacaa
      721 aagcgctttg gcctgatttt gatggtgcgc atcttgaaga agcaatcgcc tcttatcaaa
      781 atcgagaccg tcgattcggc ggtgtaaaaa aaacagaaga tgagaaaggg gatcagtcat
      841 gaaacaacgt gtggtcacag cagccgtggc gctagtcttg tttattccga ttatatggat
      901 cggaggcata gcggttgagc ttacagcagc aatcttagca gtaataggag tttacgagct
      961 gtttcggatg aaaggattga cactcttaag ttttgaagga gttttatccg ccgttggtgc
     1021 agtgttactt gttttgccaa aagaaagatg gtttttcttt cttccagaga atgtaagtac
     1081 atttatgtta ttttatctta tagttatgat tcttttagga acatcagtta tttctaaaaa
     1141 tacgtatacg attgatgaag caggttttcc tgtgcttgtc agcttgtatg tgggaatagg
     1201 tttccagaat tttgtcaatg cacgaacaga aagcttggct gttttgatat ttggtctatt
     1261 tattgtttgg gcaactgata tcggagcata tatggttggt cgacgttatg gtcagcataa
     1321 gttaagtcct gatatttcgc ccaacaaaac gattgaaggt gcgctcggtg gtattggtag
     1381 tgctgttgta gtcgcatttc tttatttatt gctataccca gcaaaagaac tatttaatca
     1441 tggtatgttt gtcatgcttc tgatgacgat tattttttca gcagtcggac aatttggtga
     1501 tttggtcgaa tctgcaatca aacgtcatta tgatgtgaaa gactcgggaa atatcttacc
     1561 aggccatggt ggaatcttag accgtttcga cagtttgctg tttgtatttc ctatcatgca
     1621 tttatttggc attatttagt aagtatacct gcgtttcttt gtgaatttca taaaagacgc
     1681 aggtttttat ttttcattat attgatacta ttggtgtctt tggtatacta tggtaaaatt
     1741 atagtgtttt atcgttgttt tataaaaaac acaaaaaaca agtaaaggag aaaaaagcta
     1801 tgaaaacgat tctgacattt atcatcgttt ttggtatatt agtgattgtt catgagtttg
     1861 gtcatttctt ctttgcgaaa cgatcaggaa tcctcgttcg cgaatttgct atcggtatgg
     1921 gtccgaaaat ttatgggcat caagcaaaag acgggacaac atatacgctt cgcctattac
     1981 ctattggcgg ttatgttcga atggcaggca atggggatga tgaaacagaa atggcaccag
     2041 gaatgcctct ttctctttta ctaaattctg acggcatcgt agagaagata aatttaagta
     2101 agaaaatcca attgacaaat gctatcccga tggaattgag ccgttatgat ttagaagatg
     2161 agctgactat cactggttat gtgaatggag acgaaacaga agtagtaacc taccctgttg
     2221 atcacgatgc gacgattatt gaaaacgacg gaacagaaat cagaattgcg ccaaaagacg
     2281 tacagttcca atcggctaaa ttgtggcagc gtatgctgac caactttgct ggacccatga
     2341 ataactttat tttggcaatc ctgttattca tcattctggc atttatgcaa ggtggtgttc
     2401 aagtgacgaa cactaatcgc gtaggagaga tcatgcctaa tggagctgcg gcagaagcag
     2461 gcttaaaaga aaatgatgag gtagtcagtg tggacggaaa agaaatccat tcatggaatg
     2521 atctgacaac agtcattacg aaaaatccgg ataaaacatt agactttaag atcgaacgag
     2581 aaggtcaggt gcaatctgtc gacgtgacac cgaaatctgt agagtcaaat ggcgaaaaag
     2641 ttggccaact tggtataaaa gccccaatga atacaggttt tatggataag atcattggag
     2701 ggacgcgtca agctttcagc ggttcattgg aaattttcaa agccttagga tcactattta
     2761 caggtttcag tttagataaa ttaggcggac ctgtcatgat gtaccaattg tcttcagaag
     2821 cagcaaatca aggtgtaaca actgttatta gtttgatggc gcttctttca atgaatctcg
     2881 gaatcgtcaa tctgcttccg attcctgcct tagatggcgg gaaattagtc ttgaatattt
     2941 ttgaagggat tcgcgggaaa ccattaagtc aagaaaaaga aggaatattg acattagctg
     3001 gctttggatt tttaatgttg ttgatggtgc tagttacatg gaacgatatt caacgattct
     3061 ttttctagaa aaagttgaca aatagggtat aataacaaac gataactatt ttgaggaaat
     3121 aaaggagtat atagaatgag acaatcaaaa atgttgattc caacattacg cgaagttcca
     3181 aatgatgcag aagtattaag ccatcaaatc ttgctaagag ctggatatat ccgccaagta
     3241 tcagcgggta tttattcata tctgccatta gcaaatcgtg tattggaaaa actgaaacgc
     3301 attatgcggg aagaatttga aaaaatcgga gcggtagaaa tgctgatgcc tgccatcttg
     3361 ccagcagaac tttgggaaga atccgggcgt tacgaaacat atggaccaaa cttgtatcgt
     3421 ttcaaagacc gcaatgatcg aaaaatgatc cttggaccaa ctcacgaaga aacttttact
     3481 gatttgatgc gtaatgaaat caattcatat aagaaattac ctttaaatct ttatcaaatc
     3541 caacctaaat atcgtgacga aaaacgacct cgcttcggtt tgcttcgcgg aagagagttt
     3601 atcatgaaag atgcgtattc tttccatgca acggaagaaa gtttggacga gacatataaa
     3661 gattacgaaa aagcctacac cgaagtattc aagcgttgtg gcttagaatt tcgctcgatt
     3721 atcggagacg gtggcgcaat gggcgggaaa gactcaaaag aattcatggc gatctcagaa
     3781 atcggggagg atacgatctg ttattcgact gaaagtgatt atgctgcaaa cttggagatg
     3841 gctacaagct actatgtatc aaaaaaatcg catgaaacac agcttgaatt ggagaaagta
     3901 gctacaccta acgtaaaatc aattgacgaa gtagcagatt tcttttctgt tgaacctcaa
     3961 aaaatcatca agtctgtatt gttcattgcg gatgaacaac ctgtccttgt tttggtacgc
     4021 ggagaccatg aagtcaatga cgtaaaactg aaaaactttt tatctgctga ctttttagaa
     4081 gaagcaacag aagaagaagc acaaaaatat ctaggtgctg agtttggttc tgtcggacca
     4141 gtcggtgttt cagatgacgt aaaagtatat gcagaccgtt atgtccaaga tttagcgaat
     4201 gccattactg gagcgaacga aacaggattc cactataaaa atgtgaatcc agaacgcgat
     4261 ttcactgtac tttcttatga agatcttcgt tttgtacaag aaggagatcc ttcaccagac
     4321 aacaatggtg ttcttgcctt tactcgagga atcgaaatcg gacatatctt caaaatcggc
     4381 actcgttaca gtgaatctat gggagcaact gtactagacg aaaatggacg agaaaaattt
     4441 gtgattatgg gctgttatgg tatcggtgta agtcgtttat tatcagcaat cgtagaacaa
     4501 cattcagatg aacaaggaat caactggcca aaaggaattg cgccatttga tttgcatatc
     4561 gtccaaatga atctaaaaga cgaataccaa acaaacctga cgaatgaact agaagaagcg
     4621 atgacaaaag ctggctatga agtactggtt gatgacagaa atgaacgcgc aggagtgaaa
     4681 ttcgcggatt ctgatttgat tggctgtcca atcagaatca ctgtcggtaa aaaagcagtg
     4741 gatggaatcg tagaagtgaa aatcaagaaa actggcgaaa tggttgaagt acgtaaagaa
     4801 gaattggtga atactttacc aatcctcttg aaccaaggcg aataataagc gttaaaacac
     4861 agaaaataaa aatagctgaa tcagctgttt ttgacaatct gatgattcgc ttttaaaatt
     4921 ggaactgcga tttgaatcgc agttccaatt ttttaatgta aaactcagaa aaaacgagta
     4981 tactaaaagg tactgaaaaa gaagaggata agatgcgatc aattgcattg tttctcatca
     5041 gaaagggcgt atatctttgt cagaaaaacg agatttattt gaaaaactgt tggaacaaat
     5101 ccaactagag gaagcagaaa aaaatcatcc actgcttact gctggtgaaa tggatagtgt
     5161 agtggtccat cgtaaatcgc gtttatggga atttacgctg catttttcca aaattttgcc
     5221 tattatgcta tatcggtcat tgactcagca tctgactatc gcttttaaag atattgcaca
     5281 agtcaaactg aatatcttag cagatgacca gacattcgat gaacagctct tgcaagatta
     5341 ctgggcccaa gctttggaaa atcagcaatg cgatacacca ctagcacagc aagtattgaa
     5401 aacgcaggta cctgtgataa aagatcgtaa agtcatcttg ccaatcgaca gtacaggagc
     5461 tatttcttat ttgaagcaac aatacttgcc attagtagaa gagttgttcg tcagttacgg
     5521 attcccaaaa tttcgtatcg aaccagaagt ggatgagcag caagcggaac gagtattgaa
     5581 actttttgaa gaacgcaaac aagaacaggc agaagccttt atgaagcaag cagctgaaag
     5641 tctagttgtc cacgaacaaa agaaaaagga aagaaaagag caatcgccag ctcttgaagg
     5701 acccatccac atccagctag gacgaaatat tccagccgat gaaccaacga caccaatgat
     5761 cagtatcgtt gaagaagaac gaagagtcac acttgagggg tacgtattcg acaaagaagt
     5821 cagagaacta cgttcaaaac gcaagatctt gactttgaaa atcacagatt atacttcatc
     5881 atttatcgtc aaaaaattct cgaacggcga aaaagatgaa caagtatttg atgcgatcag
     5941 tgtcggtagc tggttaaagg tacgaggaag tattcaagaa gatacgtttg tccgtgattt
     6001 ggtcatgaat gcgcaagata tcatagaagt caaacataca cctcgcaaag attatgctcc
     6061 tgaaggggaa aaacgagtag agcttcatgt tcatagcaat atgagtacga tggatgcaac
     6121 aaacagcatc tctgatcttg tggcacaagc agggaaatgg ggacaccgag ctatcgccat
     6181 tacagaccac ggtggtgcgc aagcctttcc ggaagcacat agtgctggaa aaaaagcagg
     6241 tgtgaaaatc ttatacggcg tggaagcgaa tgtcgtagat gatggtgtcc caattgctta
     6301 taatgatgca catgaagcat tgagtgaagc cacatatgtc gtatttgacg tggaaacgac
     6361 aggattgtct gcagtatatg acacgatcat cgagctagcc gcagtcaaaa tgtataaagg
     6421 aaatgtgatc gaaagctttg atgaatttat cgatccagga catcctttgt cacgaacaac
     6481 ggtagatctg actggtatca cggatggaat ggttcgagga tcaaagtcgg aagaagaagt
     6541 attgcggatg ttccttgaat tttcaaaaga tacaattttg gtagcccaca acgcggcttt
     6601 tgatatgggg ttcttgaata ctagctacgc aaggtatggt attcctgaag cggccaatcc
     6661 agtcattgat acgttggaat tagctagata tctgtatcct caattcaaac gtttcggatt
     6721 aggtgtgtta tctaaaaaat ttggtgtcag cttggaacag catcaccgag caatctatga
     6781 tgctgaagca actggtcact tagcatggat ctttgtgaaa gaggcaatgg acaatcataa
     6841 catgctttat catgatcagc taaatgagca tatcggagaa ggagattctt acaaaagggc
     6901 tcgacctttc catgtaacga ttttagcaaa aaatcaggca ggactgaaag atttgttcaa
     6961 actgatttca atgtcgaatg tcgaatactt cgaacgcgtc ccacgaatac cacgctccca
     7021 attgaaaaag atgcgtgaaa atctattgat cggctcagct tgtgataagg gagaaatatt
     7081 cgaagccatg atgcaaaaag gggtagaaga ggcaagaaac cgagcaaaat tctacgatta
     7141 tattgaagtt atgccaaaag cggtctatgc gcctttgatc gagcaagaat tggtaaaaaa
     7201 tgaacatgat ttggaagaaa ttatccaaaa tttggtagaa atcggaaaaa gtttggataa
     7261 aatcgttgtt gcaacaggta atgtacatta tctgaacgaa gaagatgcca tttaccgaaa
     7321 aattttgatc aattctatgg gcggagcgaa tccgttgaat cgtcatagtt tgccggatgt
     7381 ccatttccga acaaccgatg agatgctaac tgcatttcac tttttagggg aagaaacagc
     7441 gaaagagatc gtagtagaaa atacgaataa aatcgcagat atctgcgaag aagtgattcc
     7501 agtcaaagat gaactttata caccgaagat tccaggttca gaagatgaaa tatcagaatt
     7561 gagttacaca aaagcaaaac aaatgtacgg agatccgtta cctgagatca ttcaaaaacg
     7621 attaaaaaaa gagttgaatt ctattaatgg taatggcttc tcggttattt atctgatcgc
     7681 ccaaaaacta gtgcataaaa gtaatgaaga tggctatcta gtaggttcac gtggctctgt
     7741 tggctctagc tttgttgcga cgatgacagg aatcactgaa gttaatccgt tagcccctca
     7801 ttattattgt ccagagtgcc aatattccga gttttttgaa gatggtacgt atggttctgg
     7861 atttgacatg ccagaaaaac aatgtccaaa atgtggtgca cgcttaaaca aagacggtca
     7921 tgatattcca tttgagacat ttttaggatt ccatggagac aaagtgcccg atattgattt
     7981 gaacttctca ggtgattatc aagcagaagc ccataactat acgaaagtat tgtttggcga
     8041 agactatgtt taccgtgccg gaacgattgg tacagtcgcc gacaaaacag catatggcta
     8101 tgtaaaagga tacgaaaggg acaataatct gcagtttcga agtgcagaag tcgacagatt
     8161 agcaaaaggc gctactggag tcaaacggac aaccggccag catccagggg ggatcatcgt
     8221 tattcccgat tatatggatg tctatgattt tacgccgatc caatatccag cagacgatca
     8281 aaattcggaa tggaaaacga ctcattttga ttttcactcg attcacgata atgtattgaa
     8341 attagatatt ctagggcatg atgacccaac tgttattcgg atgcttcaag atttatcagg
     8401 gatcgatcca caaacgatcc ctacggatga cccggaagtg atgcggatat ttgccggtcc
     8461 tgaagtactt ggagtaagtc aggaacaaat ttattccaaa acagggacat taggtattcc
     8521 agaattcggc actcgttttg ttcgagggat gctagaagaa acgcatccta caacttttgc
     8581 agaactactg caaatttccg gactatctca tggtacagac gtatggttag gaaatgcgga
     8641 agaattgatt cgacgaggag atgcgacact ggcggaagta atcggctgtc gtgatgatat
     8701 tatggtttat ttgatccatg ctggattaga tagcgggatg gcatttaaaa tcatggaaac
     8761 tgttcgtaaa ggacaatgga acaagatccc tgatgaattg cgagaaacct atctatcggc
     8821 gatgaaagaa aataatgtgc ccgactggta tatcgattct tgttcaaaaa tcaaatatat
     8881 gtttccgaaa gcccatgctg cagcttacgt attgatggct cttcgtgtcg cttatttcaa
     8941 ggtttatttc cctattttgt attactgtgc atacttttct gtacgtgcag acgactttga
     9001 tctagtctcc atgtgcaaag gaaaagatgc tgtgaaacaa gcaatgaaag aaatcacgga
     9061 caaaggatta gatgcttccg taaaagaaaa gaatcaactg acagtgctgg agttagccaa
     9121 tgaaatgctg gaacgaggat tcaaatttgg aatgattgat ttgtataaat cggatgccgt
     9181 taactttgtt attgaaggtg atacgttgat cgcaccattt agagctgtcc caagtttggg
     9241 aaccaacgtg gcaaaacaaa tcgttgaagc aagaaaagac ggtccatttt tatccaaaga
     9301 agatcttgcc acccgcggta aagtttcaaa aacattgatc gaatatatga acgacaatgg
     9361 cgtattgaaa gatctaccag atgaaaatca attatcgtta ttcgatatgc tataagcatc
     9421 tgttttcctg tctgcttgta attttaagca gagtatggta tagttcttat aggtaatgat
     9481 actaactgtg agtgagcgga tttttcgctc actcttttta atggaattac gcacttaatt
     9541 ttttaaggag gcgaaacttt tgagtagcgt cgttgaaaca gtcacagaaa tggtgacacc
     9601 gattttagat gaacaaaatt ttgaacttgt cgaagtcgaa tttgtaaaag aaggaaaaag
     9661 ctggttctta cgagtattta ttgacaaaga aggcggtatc gatatcgaag aatgcgcctt
     9721 tgtcagtgag aaactgagtg aaaaacttga tgcagcagat ccagatccga ttcctcaagc
     9781 ttatttcctg gaggtatcct ccccgggagc agagcggcca ttgaaaaaag aaagcgacta
     9841 tgaaaaggca cttggtgatt atgtccatgt ctccttatac caaccagtag atggcgaaaa
     9901 acaatatgaa ggatttcttc aatcatttga tgctgaacag cttacattga aaatacgcat
     9961 aaaaacacga gaaaaagaat tgacttttga ccgaaaaaat attgcaaaag ctcgcttagc
    10021 tatccaattt taatcacgga gaggaatcag aaaaaatgag taaagaaatg ttaaatgcgt
    10081 tagacgcttt agaagctgaa aaagggatct caaaagaaat cgtgatcgat gctttagaag
    10141 cagcattggt ttctgcatac aaacgtcact atggccaagc acaaaacgtc gaagtggaat
    10201 ttgatcagaa aaaaggaaag atccatgtct atgcggtaaa agaagtaacg gaagaagtga
    10261 tggattcaca gttggaagtc tcattgaaag atgcactttt aatcaatcca gcatatgaga
    10321 tcggtgatac gatccgtttt gaagtaacac caaaagattt tgggcggatc gctgcgcaaa
    10381 cggctaaaca agttatttta cagcgtgtac gtgaagcaga aagaacgatt atttataacg
    10441 aattcagtgc ttatgaaaaa gacatcatgc aaggaatcgt cgaacgccag gataaacgtt
    10501 atatttatgt caatcttggt aaaattgagg cagttctttc taaacaagat caaatgccaa
    10561 atgaatttta tcaaccacat gatcgaatca aagtttatgt ttcccgagta gaaaatacgt
    10621 ctaaaggtcc gcaagtattt gttagccgca gccatccaga cctattgcgc cgcttatttg
    10681 agcaagaagt accagaagtc tatgatggaa tcgttgaaat tgtcagcatc gcccgtgaag
    10741 ctggcgatcg ttcaaaagtg gcagttcgct caaatgatcc aaatatcgat gcagtcggta
    10801 catgtgtcgg gccaaaggga caacgagttc aagcaatcgt caatgaatta aaaggcgaaa
    10861 acatggatat cgtcgaatgg aatgaagatc cagctgtatt cattgcaaat gcattgaatc
    10921 ctgcacaagt catggatgtg atcttcgatg agacaaatcc aaaggcctgc acagttgtcg
    10981 ttccagacta ccaattatcg ctagcaatcg gtaaacgcgg acaaaacgca cgtttagctg
    11041 ctaaattgac taaccataaa atcgatatca aatcagaatc tgacatgacg gaattttatg
    11101 aaaatcaaga acaacaaaaa gaaacagaag aactgcatga tgaagcaatc gttcagtcag
    11161 atttaacaga cgatgaatat gaaacaattg cattcaacaa tgaaacggtc gaagaaaaac
    11221 cagaagctta actttagtaa ggaggcaaag agatgaaaca aaggaaaatt cctttacgca
    11281 aatccgtggt ttcaggtgaa atgaaaccta aaaaagagat ggtccgaatc acccgttcaa
    11341 aagaagggga agtatcgatc gatccaactg gaaaaatgcc tggacgagga gcgtatgtct
    11401 cacttgagcc ggaagaagta caacaagctt gggataaaaa aattctcgat cgagtactcg
    11461 aaacaacatt aacagatgag ttttatcaag aacttcttga ctatgtcact caccaaaaag
    11521 cacgtaaaga gttatttgga aaatgaatac agttaatcgg caaaaagcca tgaatctcat
    11581 cggtctagcg atgcgcgcgg gaaaactaat cacgggtgaa gaactgacca ttgctgaaat
    11641 ccgcaaacaa aaagcaaaat tggtgtttgt ggcaaccgat gcaagcgaga atacaagaaa
    11701 aaagataaaa gataagagtt catattatga agttccttgc tttgagctat tttctgaagc
    11761 agaaatcacg cagatgatcg gaaaacctcg caaggtattc ggaataatgg atgccggatt
    11821 tgcgaagaag accaaggagc taattgaagg ttaggaaggt gattgcatgg gcaacaaaag
    11881 aatctatgaa ttagcaaaag aactcaaaaa atctagtaaa gacgtcgtgg aaaaagcaca
    11941 acaacttggc atcgatgtga aaaaccatat gggtgcgatt actagcaatg atgagaagaa
    12001 attgcaacaa gcgttcaaaa acccaagcac gaaacaacaa gctgcacaac aagcaagcag
    12061 aaaaccagcc aatcaacagc cgaacgatca acgtaaaaaa gaaacaacga acaatcaaca
    12121 aaaaaataaa aataaccgca gttatcaaga tcgcggtcaa gggagcggtc aagtgaatca
    12181 aggaaaaaaa ccaacaacaa accaaaataa tacgggaaat accagcagcc aaaatcgtca
    12241 aggaacgagc caaggcggca atcaaaaccg tactaacaat aacaacaaac caaacaataa
    12301 caatcgcggc aaatttaata ataataaccg taatcgttat aataaaaaag gtaaaaaagg
    12361 caaacagcaa caatcaacta aaccagctgt accaccacgc aaattccgtg aattgccaga
    12421 tgtattagag tatactgaag gtatgaacgt agcagatatt gcgaaaaaaa ttcatcgtga
    12481 gccagcagaa atcattaaaa aattatttat gcttggtgtc atggtcaatc aaaaccaagc
    12541 actggataaa gatacgattg aactgttagc gacggactat ggcatggaac cacaagaaaa
    12601 agtccaagtt gacattgctg atatcgataa attcttcgag gcagaagaag tcgatccaga
    12661 taaattagtc tcacgtccgc cagtcgtaac gattatggga cacgtagacc atgggaaaac
    12721 aactttattg gatacattac gccattcacg tgtaacaagc ggcgaagccg gcggaatcac
    12781 acagcatatc ggtgcctacc agatcgatat tgatggtaaa ccaatcactt tcttggatac
    12841 accaggacat gcggccttta caagtatgcg tgcacgtgga gcaagtatca cagatatcac
    12901 gatcttagtt gttgctgcag atgatggcgt catgccacaa acagttgaag caatcaacca
    12961 tgccaaagct gcaggcgttc caatcatcgt cgctgtaaat aaaattgata aaccaggtgc
    13021 aaaccctcaa catgttatgc aagaattaag tgagtatgag ttaattcctg aagcatgggg
    13081 tggagaaacg atctttgttg agatctcagc gaaattcggc caaaacatcg aagaattgct
    13141 ggaaatgatc ctgttagtcg cagaagtaga agatttaaaa gcagatccaa cacaacgcgc
    13201 aatcggaaca gtgatcgaag cacgtcttga taaaggtaaa ggtcctgtgt caaccttact
    13261 tgtccaagaa ggaacacttc atgttggtga cccaatcgtt gtcggtaata cttacggacg
    13321 tgtccgtgta atgacaaacg atcttggccg tcgcgataaa gaagcaggac ctgcaacgcc
    13381 agtagaaatc actggcttga atgatgtacc tcaagcaggt gaccgtttcg tagtcttcga
    13441 agacgaaaag acagcacgtg cagcgggtga agaacgcgcg aaacgtgcat tattagaaaa
    13501 ccgtgctgcc acaagccgtg tgactttgga taacttattt gaaagcctga aagaaggcga
    13561 attgaaagaa gttaacgtaa tcatcaaagc agatgtacaa ggttcagcag aagcactggc
    13621 tgcatcattg aagaagattg atgtggaagg tgtccgtgta aaaatcgtcc attcagctgt
    13681 cggagcaatc aatgaaagtg atgtaacctt agcagcagcc agcaacgcta ttatcatcgg
    13741 tttcaacgtt cgtcctacac cgcaggcaaa agtgcaagct gatactgaag aagtcgatat
    13801 tcgtcttcac cgtatcatct ataaagccat cgaagaaatc gaaactgcga tgaaagggat
    13861 gctagaccct gaatttgaag aaaaaatcac cggtcaaatg atcgttcgtg aaaccttcaa
    13921 agtttctaaa gttggtacga ttgcaggtgc ctatgtaaca gaaggatata ttcgtagaga
    13981 tagcggtgtc cgtgtaatcc gtgacggaat cgtaatctat gaaggccaac ttgcaagtct
    14041 gaaacgattc aaagatgacg tgaaagaagt caaaatgggt tatgaatgtg gtgcaatgat
    14101 tgaaaaattc aatgacatca aagtagatga cgtaattgaa ggatttatca tggaagaagt
    14161 caaaaatgac taaaaactga taaaaggagg ccaatcatta tggctaacta ccgtgaccgc
    14221 cgagtcggtc aagaaatctt aaaagaagtc aatgatattt tgcgcaaaaa ggtccgtgac
    14281 cctcgtgtgg agaacgtaac gatcacagat gtgaaagtta cgggggactt acagcaagca
    14341 acgatctatt acagcatttt atctgactta gcatcagata agaaaacagc acaagatgga
    14401 ttgaataaag catcaggatt gattcgtcga gagttagggc acaatctaag tatttataag
    14461 acacctgaat tgttctttga acttgatgaa tcagttgtct atggcaacca tatcgatgaa
    14521 ttgcttcgcg acttgaacaa agactaatca aaaactcccg caaatcctgt gaaatcaggg
    14581 gttgcgggat ttttttgttt tcattaagac gtattaccta agagttccat gactttagtc
    14641 ttgcggcgtg tcagctaacc aaaaaagaga aaaagaaaag taaaaaattc gactttcttt
    14701 tttctagtag tatataaatt tttgcatata gataaagatc aagataccga taacggaagc
    14761 tagattgtaa tctattttag tgacagtcag gtatagccaa gtaactagaa gatcagtgga
    14821 acctgcacca taaaagtttg agtttgtcgg tccgttgaca gttaaaagaa aaatcacgtt
    14881 gaagttattg atatttccga ttacgccgct tcgatttggt cacgatccaa attttggata
    14941 atccctgtag aaattagcat agtagctggg ataccaattc acatattgac aaagataaca
    15001 gagactttta cataaaacgg atcaatcagg aaaggaattg ctcgatccag ccccagtttt
    15061 ccagcaatgt gttaactggc ccagcgtcat tcagcaaatt atgcatgatg aggagcgaaa
    15121 tgaactgcgg aacagccatt gtgataacga caagtttttt ggatcaatgt aaaaaacgtc
    15181 agcgaaagga aaaatttcgt ggttttttat acctgttcat ttggctagat ataaaatcca
    15241 aacaaatgaa cagaagtgct tattaggttt caattgggag tatctgcgct agaataagag
    15301 taataatgaa aaggagtgtt atgtatggat tattctagta aagaatactt tgataaaatg
    15361 acggcttggt ggcgtgctgc taattatctt tctgttgggc aactttatct taaagataac
    15421 cccttactga gacgtacact gaaacccgaa gatgtcaaaa aacatccaat cggtcattgg
    15481 ggaacgattc ctggccaaaa ttttatttat gtccatttga accgagtcat caataaatat
    15541 gatttgaaca tgttttatat tgaagggcca ggacacggcg gacaagtcat ggtatcgaat
    15601 gcttatttag atggaagtta cacagagatc tatccagaag taacggaaga tgagacagga
    15661 atgcaaaaat tattcaaacg attttcattt cctggaggca ttgcttctca tgcagcacca
    15721 gaaacaccag gttctatcca tgaaggcgga gagcttggat actctctttc acatgcagtc
    15781 ggagctgtat tagacaatcc tgaagtaatc tctgctgtcg tgattggtga tggtgaagca
    15841 gaaaccggtc ctttagctgg aagctggttc tcaaatgtct ttatcaatcc agtgatagat
    15901 ggagctgttt taccgatcct tcatttaaat ggtgcaaaaa tagccaaccc aacgatcttg
    15961 gcacgtaaat cagatggaga attagcaaat tactttaacg gacttggctg ggaacctttc
    16021 tttattgaag gaaatgattc agaaaaatta aatcctgtta tggctgaaaa aatggatcaa
    16081 gcaatcgaga aaattaaatc catccaaaaa gaagctcgcc tgaagacagc aacagatgtc
    16141 gtgatgccaa aatggccagt attgatcgta cgcacaccaa aaggatggac cggtccaaaa
    16201 gaatgggacg gggaaccaat tgaaggaacc ttccgagcac accaagtacc gattcctgta
    16261 gatcaagaac atatggatca tgcagatgct ttactccgtt ggctgaaatc ctatgaacca
    16321 gaaaaattat ttgatgcaca aggacgtatt ttagaagaaa tcagagaaat cgcgcctact
    16381 ggtgaccaaa gaatggcaaa aaatcctatc accaatggtg gaatcgatcc aagaccgctt
    16441 attatgccag attggaaaaa atatactttg caatttgaaa aaccaggctc cgttaaagca
    16501 gaagatatga ccgaacttgg taaattcgtt cgcgagatca ttgagaaaaa tccagaaaat
    16561 ttccgtatat ttggaccaga tgaaacaaaa tcaaaccgat tgaatcaagt tttcaaaacg
    16621 acaaatcgcc aatggatgga aaaaatcgaa ccagaaaatg atgaatggct ttcgccatct
    16681 ggtcgcgtca tcgacagtca attatcggaa caccaagatg aagggttctt agaagggtac
    16741 gttctgactg gacggcatgg ttttttgcaa gttacgaatc attcttgcga gtggttgact
    16801 ctatgttgac tcagcacttc aaatggatgc ggaaatcaca tgacctttct tggcgaaatg
    16861 attatccttc gctaaatctc attgcaagct caacagtctt ccagcaagat cacaatggat
    16921 atagccacca agatccagga atattgacac atttggcaga aaagaaggcg gagttcatcc
    16981 gtgaatatct gcctgcggat gcaaatacat tgctcgcagt catggataaa gcattccgct
    17041 cgtcagaaaa aatcaatttg atcatctctt ccaaacatcc gcgtgctcaa ttttactcag
    17101 cagaagaagc agccgtctta gtgaatgaag gcttgaaaat cattgattgg gcttcgacag
    17161 caaaagagga ggaacctgaa ttggtcattg ctgctgcagg aacggaatct aacttagaag
    17221 cattagcagc tgtaactttg ctgctggaag aattcccaaa actaaaaatt cggttcatca
    17281 atgtcgtaga tttattgaaa ttacgtcatc caagtcaaga tccgcgtggg ttgagcgatg
    17341 aagaatttga tcaatatttt acaaaagaca aaccgatctt atttgctttc cacggttatg
    17401 aaacattggt ccgcacgatt ttctttgatc gtcataacca tcatttaatg attcatggct
    17461 acaaagaaaa tggagatatc acgacaccat ttgacatgcg tgtagtaaat gaacttgatc
    17521 gttatcattt ggcaaaagac gcagctttaa aaatcaaagg aagtcaagca gaagattttg
    17581 cgaaaaaaat ggatcagaaa ttacaagaac atcaaaacta tattcgtgaa aatggtattg
    17641 atctgccaga agtattggat tggaaatgga aaaatcttga ccaataaaat gataaaatcg
    17701 atcccgcaaa atagaatttt ttacattgat taagaaactg aaaaaataaa taaaaaaaac
    17761 ggaaaaaaga ctggaagaat ggccagtctt ttttttcgtt tttgggaagt gaaaaccaaa
    17821 ctatttacct gtaagccacc cataaatcca atcaaatggt gcaaagaaaa aatcttccat
    17881 tctattccct cctttaaaaa agcataacat gatttctatt tacgtgagca tgaattttgt
    17941 tacgttttta ttgccgaaaa ataaaaaccg ttcattatga tgtcaaattc gtcagtaatt
    18001 gatgcaatgt gttataataa ttcagttaaa tcaaaaatga aatggagaaa agacatggac
    18061 gggttattac ctttatggaa agaacgcgga atgacaagtc atgattgcgt attcaaatta
    18121 agaaaaatct tacatacgaa aaaaatcgga catgggggta cgcttgaccc agacgtagac
    18181 ggtgtgttgc ccatttgtat tggaaaagcc acaaaagtta tcgaatattt gacagattct
    18241 gggaaaatct atgtaggcga gataactcta ggtttcagta caacgacgga agatcgatcc
    18301 ggagaacggg tagaaacaaa accagtagat atgtctttat ctgaaaaaaa aatagatgaa
    18361 gtgatggctt catttattgg tgagatcacc cagataccac caatgtactc agcggtaaaa
    18421 gtcaacggta agcggttata tgaatatgcc cgcaataatg aaacagtgga gcgtcctgtt
    18481 agaaaggctc agatttaccg ttttatccgg acaagtgaat tgacaagaga cgaagtgaca
    18541 ggaaccattt cttggcgctt cgaagtagaa tgcgggaaag gaacctatgt ccgcacatta
    18601 gctgtagata caggcaagaa acttggttac cctgcgcaca tgtctgatct gacccgaaca
    18661 gcaagtgccg gtatgtccca gaaggatgct ctgacattag aagaagtagc tcaagctgtg
    18721 aaaaatggga caatcgatga ttgtttgctg ccaatagaaa aaggagtgga acggttcaaa
    18781 cgagtagata ttactgatga agtatggcag aaagtaaaaa acggtatgag acttgattat
    18841 actgtatttg cgctcccaga aatgccgtca gaagaaattg ctttgtttta taaaggaaaa
    18901 gttgtcagta tttatcagcc aaacccgaaa gaaaaaaaca aattaaaacc tagtaaagta
    18961 ttgagaaatg aggaataacg aatgaatatc atcaagatca gacatcctta ccaatcagaa
    19021 caaattccag aagacgatgt cgttttagtg ctgggatttt tcgacggcgt gcatatcgga
    19081 caccagaaag tcataaagac aggtatggaa attgcacaaa aagaaggatt gaaattagca
    19141 ttgatgactt tcaatcagca tccatctatt gtctttaaaa agatcgatcc tagcagcgtg
    19201 aaatatttga ctagccttaa gcaaaaagaa gaaaaaatgg ccgctttggg tatagattat
    19261 ttatatgaag ttgattttac ttcttctttt gctcatttag caccgcagga atttgttgat
    19321 caatacatcg tagggttaca tgcaaaatat gctgtttccg gttttgatta tacgtatgga
    19381 ccaaaagaca ttgcagatgt cgctcatttt cctgaatatg ccaaaggaag attcaaaatc
    19441 gtcacagttt ctaaagaaga agaacaaggt gaaaaaatca gctctacccg tattcgtgga
    19501 ttactagaca gcggacaagt aaaagaagca gcaaaactat taggttgcac atatgaggta
    19561 gaaggggtag ttgtccatgg cgatgcacga ggcagactac taggattccc tacagcaaat
    19621 gtcaaagtaa agagcacggt tcatttgcct aaagaaggtg tttatgtgtc ggaaataaaa
    19681 attggggaaa catggtatcc agccatgggt tcaattggac ataatgatac attcggtgaa
    19741 ggacgggagc tgactgtaga gttgtatatt ttggacttta atcaagatat ctatggtgaa
    19801 catgtcgcgg tacgctggaa tcaattttta agagatcaag tgaaattttc tagtgtagag
    19861 gaattgattg aacaactcaa ggaggatgaa caagcaacag ctgaatattt ttctcagcaa
    19921 taaaacatta tgcataaaat ctcttgttta tgtataacta gcaattacaa taaaatgggt
    19981 gataaataag cccag
//
LOCUS       DACCXM010000047        19941 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCXM010000047 DACCXM010000000
VERSION     DACCXM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.049"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            300..608
                     /gene="rpsJ"
                     /locus_tag="IVY61_001417"
     CDS             300..608
                     /gene="rpsJ"
                     /locus_tag="IVY61_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP7970537.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            639..1268
                     /gene="rplC"
                     /locus_tag="IVY61_001418"
     CDS             639..1268
                     /gene="rplC"
                     /locus_tag="IVY61_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP7970538.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1296..1919
                     /gene="rplD"
                     /locus_tag="IVY61_001419"
     CDS             1296..1919
                     /gene="rplD"
                     /locus_tag="IVY61_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP7970539.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1919..2209
                     /gene="rplW"
                     /locus_tag="IVY61_001420"
     CDS             1919..2209
                     /gene="rplW"
                     /locus_tag="IVY61_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP7970540.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2248..3081
                     /gene="rplB"
                     /locus_tag="IVY61_001421"
     CDS             2248..3081
                     /gene="rplB"
                     /locus_tag="IVY61_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP7970541.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3124..3402
                     /gene="rpsS"
                     /locus_tag="IVY61_001422"
     CDS             3124..3402
                     /gene="rpsS"
                     /locus_tag="IVY61_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP7970542.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3425..3772
                     /gene="rplV"
                     /locus_tag="IVY61_001423"
     CDS             3425..3772
                     /gene="rplV"
                     /locus_tag="IVY61_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP7970543.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3786..4442
                     /gene="rpsC"
                     /locus_tag="IVY61_001424"
     CDS             3786..4442
                     /gene="rpsC"
                     /locus_tag="IVY61_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP7970544.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4445..4879
                     /gene="rplP"
                     /locus_tag="IVY61_001425"
     CDS             4445..4879
                     /gene="rplP"
                     /locus_tag="IVY61_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP7970545.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4869..5057
                     /gene="rpmC"
                     /locus_tag="IVY61_001426"
     CDS             4869..5057
                     /gene="rpmC"
                     /locus_tag="IVY61_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP7970546.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5082..5348
                     /gene="rpsQ"
                     /locus_tag="IVY61_001427"
     CDS             5082..5348
                     /gene="rpsQ"
                     /locus_tag="IVY61_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP7970547.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5406..5774
                     /gene="rplN"
                     /locus_tag="IVY61_001428"
     CDS             5406..5774
                     /gene="rplN"
                     /locus_tag="IVY61_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP7970548.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5811..6119
                     /gene="rplX"
                     /locus_tag="IVY61_001429"
     CDS             5811..6119
                     /gene="rplX"
                     /locus_tag="IVY61_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP7970549.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6147..6686
                     /gene="rplE"
                     /locus_tag="IVY61_001430"
     CDS             6147..6686
                     /gene="rplE"
                     /locus_tag="IVY61_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP7970550.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6705..6890
                     /locus_tag="IVY61_001431"
     CDS             6705..6890
                     /locus_tag="IVY61_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP7970551.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6926..7324
                     /gene="rpsH"
                     /locus_tag="IVY61_001432"
     CDS             6926..7324
                     /gene="rpsH"
                     /locus_tag="IVY61_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP7970552.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7356..7892
                     /gene="rplF"
                     /locus_tag="IVY61_001433"
     CDS             7356..7892
                     /gene="rplF"
                     /locus_tag="IVY61_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP7970553.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8053..8409
                     /gene="rplR"
                     /locus_tag="IVY61_001434"
     CDS             8053..8409
                     /gene="rplR"
                     /locus_tag="IVY61_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP7970554.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8430..8930
                     /gene="rpsE"
                     /locus_tag="IVY61_001435"
     CDS             8430..8930
                     /gene="rpsE"
                     /locus_tag="IVY61_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP7970555.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8945..9124
                     /gene="rpmD"
                     /locus_tag="IVY61_001436"
     CDS             8945..9124
                     /gene="rpmD"
                     /locus_tag="IVY61_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP7970556.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9168..9608
                     /gene="rplO"
                     /locus_tag="IVY61_001437"
     CDS             9168..9608
                     /gene="rplO"
                     /locus_tag="IVY61_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP7970557.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9608..10903
                     /gene="secY"
                     /locus_tag="IVY61_001438"
     CDS             9608..10903
                     /gene="secY"
                     /locus_tag="IVY61_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP7970558.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10965..11612
                     /locus_tag="IVY61_001439"
     CDS             10965..11612
                     /locus_tag="IVY61_001439"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP7970559.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11803..12021
                     /gene="infA"
                     /locus_tag="IVY61_001440"
     CDS             11803..12021
                     /gene="infA"
                     /locus_tag="IVY61_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP7970560.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12054..12170
                     /gene="rpmJ"
                     /locus_tag="IVY61_001441"
     CDS             12054..12170
                     /gene="rpmJ"
                     /locus_tag="IVY61_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP7970561.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12188..12553
                     /gene="rpsM"
                     /locus_tag="IVY61_001442"
     CDS             12188..12553
                     /gene="rpsM"
                     /locus_tag="IVY61_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP7970562.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12581..12970
                     /gene="rpsK"
                     /locus_tag="IVY61_001443"
     CDS             12581..12970
                     /gene="rpsK"
                     /locus_tag="IVY61_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP7970563.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13050..13988
                     /locus_tag="IVY61_001444"
     CDS             13050..13988
                     /locus_tag="IVY61_001444"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP7970564.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14016..14396
                     /gene="rplQ"
                     /locus_tag="IVY61_001445"
     CDS             14016..14396
                     /gene="rplQ"
                     /locus_tag="IVY61_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP7970565.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14668..15507
                     /locus_tag="IVY61_001446"
     CDS             14668..15507
                     /locus_tag="IVY61_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7970566.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15483..16352
                     /locus_tag="IVY61_001447"
     CDS             15483..16352
                     /locus_tag="IVY61_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7970567.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16342..17139
                     /locus_tag="IVY61_001448"
     CDS             16342..17139
                     /locus_tag="IVY61_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7970568.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17342..18088
                     /gene="truA"
                     /locus_tag="IVY61_001449"
     CDS             17342..18088
                     /gene="truA"
                     /locus_tag="IVY61_001449"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7970569.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            18753..18990
                     /locus_tag="IVY61_001450"
                     /pseudo
     CDS             18753..18990
                     /locus_tag="IVY61_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19134..19910)
                     /locus_tag="IVY61_001451"
     CDS             complement(19134..19910)
                     /locus_tag="IVY61_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7970570.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT         6627 a         3581 c         4290 g         5443 t
ORIGIN      
        1 aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt attttctaaa
       61 tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg tgagtttata
      121 tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta atcagaatga
      181 tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca cgaacgagcg
      241 tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga gggaacaaga
      301 tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt ttagatcaat
      361 cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct ggtccaattc
      421 cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa tacaaagatt
      481 cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg aacccaacac
      541 caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac atcgaaatca
      601 aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga atcttaggga
      661 aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca gtaactgttg
      721 tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat ggctacgaag
      781 ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc
      841 atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa aatgttgagc
      901 taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca ggagacatta
      961 ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa cgccacggac
     1021 aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg tcaatgggtc
     1081 cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc
     1141 gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat gttatcttga
     1201 tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca gctgtgaaag
     1261 ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca ttattcaaac
     1321 aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga attgaaccta
     1381 atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac
     1441 acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag
     1501 gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga ggtggcgtag
     1561 tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt cgtcgtttag
     1621 caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct
     1681 tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac ttatctatcg
     1741 actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta tcagcacgta
     1801 acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac gttgtatcaa
     1861 atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg cttgcataat
     1921 gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc ttgccatgga
     1981 tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag taaaacaagc
     2041 ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa
     2101 attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac
     2161 attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag cactaataga
     2221 taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat
     2281 ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac acctgaaaaa
     2341 tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg catcactgta
     2401 cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa
     2461 gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc agctaacatc
     2521 gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa aggactagaa
     2581 gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa tgctcttcca
     2641 ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc tggtaaaggt
     2701 ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga aggcaaatac
     2761 gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca
     2821 atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc tggacgctct
     2881 cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca
     2941 cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc tccttggggt
     3001 caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa acttattgtt
     3061 cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga gaggaggttc
     3121 accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt
     3181 gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca
     3241 gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa acatgtacca
     3301 gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc tacaagaact
     3361 tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg gaggataaat
     3421 ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc gcacttcacc
     3481 tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc
     3541 aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc
     3601 agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag taagcgaagc
     3661 ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc
     3721 tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat aaggagggat
     3781 aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc cgcgactggg
     3841 atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat ttaagaatcc
     3901 gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg
     3961 caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt attggtaaag
     4021 gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt aaaagaatcc
     4081 atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta ggtgaaggaa
     4141 tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac
     4201 gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt ctaaacggag
     4261 cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat actttacgtg
     4321 cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta ggagttaaag
     4381 tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat
     4441 aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg
     4501 ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag ctgttgattc
     4561 acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa
     4621 acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat
     4681 tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac cagtaaaacg
     4741 tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg
     4801 tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg aaatgggtgg
     4861 tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg cttgatcaag
     4921 aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca ggtcaattag
     4981 aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa actgtgttgc
     5041 gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa ggaagaaatc
     5101 aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca attaccgttg
     5161 ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat tctaagaaat
     5221 acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga attatggaaa
     5281 ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta
     5341 ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa aggaggatac
     5401 acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa
     5461 ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga
     5521 tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta
     5581 aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca tatattaaat
     5641 ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga acacgtatct
     5701 ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt tcactagcac
     5761 cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt atgtttgtta
     5821 aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac
     5881 tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac
     5941 accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa gcgccaattc
     6001 atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt ggctacaaag
     6061 aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa
     6121 agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat cttaaagaag
     6181 taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca cctaaagttg
     6241 aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa aacttagata
     6301 aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa
     6361 aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc
     6421 gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta ccacgtgtac
     6481 gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat actttaggta
     6541 ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa gtacgtggta
     6601 tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag ttgttgacac
     6661 aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa
     6721 tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact cgttgcgaac
     6781 gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt tgcttccgcg
     6841 aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa aaagtaaatt
     6901 cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag attttctaac
     6961 tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg catcaaaaat
     7021 taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat
     7081 cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg
     7141 tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg tcaaagctga
     7201 cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt ctgaaggtgt
     7261 tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg
     7321 gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg
     7381 ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca gttaaaggac
     7441 caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc gaaggaaacg
     7501 aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat ggaacaactc
     7561 gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac
     7621 taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg aacgttggtt
     7681 actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta ccagctaaca
     7741 cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct gcaaacattc
     7801 gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg
     7861 tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca tagaagccag
     7921 cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt ttacttgtgc
     7981 cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa taaaaataaa
     8041 gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt
     8101 cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt ttttcgttct
     8161 aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct agcaagtgcc
     8221 tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca agctgtcggt
     8281 aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt
     8341 ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga aaatggacta
     8401 gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa catttggaat
     8461 tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc
     8521 tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtaggg tttggtacag
     8581 gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact
     8641 tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt gcttttagtg
     8701 gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct ggtggaccag
     8761 ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct ttaggctcaa
     8821 atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag
     8881 aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa ggaggacaac
     8941 aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc ctcaaaacca
     9001 acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg ttaagccttc
     9061 taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg ttgaagaaat
     9121 ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg aaacttcatg
     9181 aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat
     9241 ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg
     9301 gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta ccaaaacgag
     9361 gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta ttgaaccgct
     9421 ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc gtgaaaaacg
     9481 aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta actgtgaaag
     9541 cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga tcaatcgagg
     9601 tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta gatcgaaaat
     9661 cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg
     9721 cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca tgttgaactt
     9781 ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt caccatacat
     9841 tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat ttgttgaatg
     9901 gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt
     9961 agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat acgctcaatt
    10021 aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga ttctaacagc
    10081 cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta tcggaaatgg
    10141 cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta tcagagaaat
    10201 ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg ctatttttgt
    10261 ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt tccaacaagc
    10321 tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta caagcagcta
    10381 tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta gttcgtttat
    10441 tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg aaaactggta
    10501 caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga tttatacgat
    10561 tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg agaaattagc
    10621 ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta aggggactga
    10681 agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct tccttggttt
    10741 agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat caatcggatt
    10801 aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga aacagttaga
    10861 aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg tgttgaggtt
    10921 tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac ctcattttaa
    10981 tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat gcttatggta
    11041 ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg
    11101 gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa gtaacaaatg
    11161 gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta ttagatggct
    11221 ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa
    11281 aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa cgtctagccg
    11341 gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac cctacaaaag
    11401 ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa gatgataaac
    11461 ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca atcttggctt
    11521 attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat
    11581 ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg acttgcatta
    11641 tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc gacttctaga
    11701 gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt tccgccattt
    11761 tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa agaagatatg
    11821 attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa agttgaacta
    11881 gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt
    11941 atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa ccgtggccgt
    12001 attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt atcatgaaag
    12061 taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg
    12121 ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa ttggaggtgc
    12181 tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg tagtagtttc
    12241 acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag ctgatgctgg
    12301 tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc
    12361 agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa
    12421 acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg
    12481 tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg
    12541 caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa
    12601 tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc attctacatt
    12661 caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt ggtcatcagc
    12721 tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc aaatggcagc
    12781 agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag taacagttaa
    12841 aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag gtttagaagt
    12901 gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc caaaacgccg
    12961 tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg aacaagacac
    13021 ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca agaatcgcaa
    13081 aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa agagggtatg
    13141 ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca ggtgctgcca
    13201 tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa ggtgttagag
    13261 aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg tatggccaag
    13321 aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct ggcgatatta
    13381 tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt gtctctgaag
    13441 gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt caagcagatg
    13501 aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc tacacacctg
    13561 ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa gattttgata
    13621 aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca atgagtttgt
    13681 ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat gaagcaaaaa
    13741 atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga
    13801 tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca ggaatcaata
    13861 ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt aacttaggac
    13921 gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag
    13981 aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca
    14041 catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta atcaacgaac
    14101 gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca
    14161 ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg
    14221 aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac
    14281 tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca cgcattttga
    14341 agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt gtctaaaatc
    14401 acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt ggaagatatt
    14461 tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag taagcactca
    14521 tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt tgaaggcata
    14581 aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata gtgttagtag
    14641 gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga aaagataaat
    14701 tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt tacaattgat
    14761 aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac acttgccaaa
    14821 acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa tcaaatattg
    14881 gatgaagaaa atatttggac gatccgacag atggttggaa tggtttttca aaatccagac
    14941 aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga aaatcaaggg
    15001 attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct
    15061 gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg tgttgccatt
    15121 gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac cagtatgcta
    15181 gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga agaaagtcaa
    15241 ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa tcggatacta
    15301 gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt
    15361 cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa gagcgcactg
    15421 aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat ggtggattgg
    15481 ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca ccatttgaac
    15541 aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact gcattagtag
    15601 ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt gttaagccaa
    15661 caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat aataagaatt
    15721 taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca cagctattcg
    15781 aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag
    15841 aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa agctatttgg
    15901 aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc
    15961 ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt gatccccagg
    16021 gtagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag atcactattg
    16081 tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc tatgtgttgg
    16141 aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac attgaatggc
    16201 taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa ttaatggcta
    16261 aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac gctatcgtcc
    16321 attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta tatgcctgga
    16381 gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt ttattttatc
    16441 ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt tttcacatta
    16501 ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat tcgcccattg
    16561 atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg cggagaaact
    16621 tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg cctctttatt
    16681 ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac tacaccgcct
    16741 ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt acggttccca
    16801 gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac attgatggac
    16861 gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac
    16921 ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt cagcagcttc
    16981 aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg tggggaagga
    17041 agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat cattgctttt
    17101 gtattattaa ctgtgctact gatctttttg agaggataaa cgttcagtgg gccgctatct
    17161 attcttttct tgctgatctg tttgcaagaa aggatgcgag cagtgtatga caagaaatat
    17221 tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga
    17281 aaaaatttta taaaaaaatc attattccag ataacctttt aggaacaaag gagagaaaaa
    17341 catggtacgt tacaaagcaa tcattgcata cgacggaaca aattttaatg gattccaaaa
    17401 acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa
    17461 tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac atgcgattgg
    17521 gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc gtttcgcgct
    17581 agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt cagaagactt
    17641 ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata tagggaaacc
    17701 acgcagtcct tttcgacgcc attatgcaag ttattttcct taccctcttg atttggaaaa
    17761 aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt tttgtgcatc
    17821 aggaagctcg atagaggata aagtacgtac gatctacgag gctaagatgg aggtaaatga
    17881 aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata agatgatacg
    17941 gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag atagtatccc
    18001 agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacatc ctgaaggact
    18061 ttatttgtat gaagtgaaat atgaataaga ggttgtgaca gaagtggtca gcttcaagaa
    18121 ataagaagga atttacgaaa attacttctc aaaattttgc aaaatttcgg cttattttcg
    18181 aaggagctac ttctggaaca ccgtgaatca gaggttgtga aaaagctgcc ctgcatcaag
    18241 taataagaac aaccaaacaa aaatagctcg tcatattttt cgtttggttg ggcttattac
    18301 cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cgttttgacc
    18361 tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa
    18421 taccgaaggt cagctgattg aacaccgttt aagaaagagg tcgtgagcct gtcatttaac
    18481 tctgagtcac aagtaacaat ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact
    18541 tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc tgtaaccaaa
    18601 aaacgatgga acgttgtttt gattgccctt aatgaggaca gtcggaacag gtttgtcatt
    18661 tttatattta aatggcacaa atttttctaa ttatttgtca tttagcgctg aaaatttttg
    18721 ctatttatct aaaaaagtga taactactta agatgaagca gaatatttac gccatacggc
    18781 atacaaccgc gaaaaatata aaaaaaagaa aacgattgaa cgcgtttttg cagtcgcaaa
    18841 ggaaaagcat ggaatgcgct acatcaagta tcgagacttg gaaaacaatc aggtgtatac
    18901 gctgattgtt tttgctgcaa tgaatctgaa aaagttgtcc acttggcagt tggaacatat
    18961 gtcttcatat cttttattat acaaaaatag caaaagcacc agtctacact cttagtaaag
    19021 aggaaactga tccttttgtc tacattctta aagtacttaa actaagtact ttattttact
    19081 ctttaggatt gatacagtag catctatccc tttattaata atcttttatt tttttaaata
    19141 aagatgttgt ctccatcttc aggcactaaa actttttcgg aaattccttt ttctttattg
    19201 aaagacttta attcttctct tgacagtccc caatggttta cagcttccat atgaacggca
    19261 ataattgtgg aatctggtat tgctttatgc aattcatata aatcgtcttt ccccataata
    19321 agagattctc caactaaaaa atgattatct ccagcatttg ctactatgat ttctggatta
    19381 tactgatcaa tagtttgttt aacagcttca taccaaactg tatctcctgt aaagtatagt
    19441 gtcttctcag tcgaatgttt gaaaacaata ccgcaaacat tacctacata attcaagatt
    19501 tctcctctgc catgtttgcc ttttgtttta accaattgta tagattcaaa aactgtgtct
    19561 tctcgtagta cctccacatt tttaaaacca tatagtcgaa tttcattcgc atcattttca
    19621 tcttgcacaa ataattttat ttctttaggt aggacctctt tcgctatatc gtcccaatga
    19681 tctagatgta aatgtgttac aataacagca tcaataccgt tgataatatc ttcgactggc
    19741 attggcaaat caactagagg attcatttga tcttttcttg gtgatggaaa aggacctttc
    19801 gaaataaatg gtggaagtgt tcctttttcc gctaacattg gatcaattaa aaatttttta
    19861 cctccatagt ctaagatagc tgttgcatta cgaatgtgat gtatattcat aaacgttgtc
    19921 tcctttttgt tgatatgaaa t
//
LOCUS       DACCXM010000048        19463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCXM010000048 DACCXM010000000
VERSION     DACCXM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.050"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(32..310)
                     /locus_tag="IVY61_001452"
     CDS             complement(32..310)
                     /locus_tag="IVY61_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970571.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            503..1159
                     /locus_tag="IVY61_001453"
     CDS             503..1159
                     /locus_tag="IVY61_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7970572.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(1601..2398)
                     /locus_tag="IVY61_001454"
     CDS             complement(1601..2398)
                     /locus_tag="IVY61_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP7970573.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(2395..3579)
                     /locus_tag="IVY61_001455"
     CDS             complement(2395..3579)
                     /locus_tag="IVY61_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7970574.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3604..4851)
                     /locus_tag="IVY61_001456"
     CDS             complement(3604..4851)
                     /locus_tag="IVY61_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP7970575.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            5066..6811
                     /gene="dnaX"
                     /locus_tag="IVY61_001457"
     CDS             5066..6811
                     /gene="dnaX"
                     /locus_tag="IVY61_001457"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP7970576.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6831..7145
                     /locus_tag="IVY61_001458"
     CDS             6831..7145
                     /locus_tag="IVY61_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP7970577.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            7170..7766
                     /gene="recR"
                     /locus_tag="IVY61_001459"
     CDS             7170..7766
                     /gene="recR"
                     /locus_tag="IVY61_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP7970578.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7830..8474
                     /locus_tag="IVY61_001460"
     CDS             7830..8474
                     /locus_tag="IVY61_001460"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP7970579.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8489..8818
                     /locus_tag="IVY61_001461"
     CDS             8489..8818
                     /locus_tag="IVY61_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970580.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8832..9770
                     /gene="holB"
                     /locus_tag="IVY61_001462"
     CDS             8832..9770
                     /gene="holB"
                     /locus_tag="IVY61_001462"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP7970581.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9806..10630
                     /locus_tag="IVY61_001463"
     CDS             9806..10630
                     /locus_tag="IVY61_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP7970582.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10623..10970
                     /locus_tag="IVY61_001464"
     CDS             10623..10970
                     /locus_tag="IVY61_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP7970583.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            11039..11911
                     /gene="rsmI"
                     /locus_tag="IVY61_001465"
     CDS             11039..11911
                     /gene="rsmI"
                     /locus_tag="IVY61_001465"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP7970584.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            12020..13141
                     /gene="dinB"
                     /locus_tag="IVY61_001466"
     CDS             12020..13141
                     /gene="dinB"
                     /locus_tag="IVY61_001466"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP7970585.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(13195..13797)
                     /gene="nrdG"
                     /locus_tag="IVY61_001467"
     CDS             complement(13195..13797)
                     /gene="nrdG"
                     /locus_tag="IVY61_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP7970586.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(14112..16301)
                     /gene="nrdD"
                     /locus_tag="IVY61_001468"
     CDS             complement(14112..16301)
                     /gene="nrdD"
                     /locus_tag="IVY61_001468"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP7970587.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16627..17394
                     /locus_tag="IVY61_001469"
     CDS             16627..17394
                     /locus_tag="IVY61_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970588.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            17384..19426
                     /locus_tag="IVY61_001470"
     CDS             17384..19426
                     /locus_tag="IVY61_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7970589.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         6103 a         3533 c         4020 g         5807 t
ORIGIN      
        1 atttcttttc catgtatgtt atacattccc cttaagctga atatgctttt aatttttgta
       61 tttgaacttc tacatcgtga taattggatg gatagcgatc aacaagttgg cgaataaata
      121 aatctcttgt agacataaac agatttaaaa attcttcgca ttcgtttgtt ggattctcat
      181 ccactgcttt gatcaattcg atcgagtttt gatacgcatc atttaaaaag actaaagaag
      241 ctttcatatc aaatcttgtt tgatatgctg tttctaatcg attaatgatt gtgttcatcc
      301 agtaattcat gttttttccc tctattctta aaaaatttta cttctgcagt ataacgaata
      361 aaaaaaagga aaaaaatcgc ataagtgatg gaaaaaggta cttaacaaat gttaatatca
      421 ttagtaaaat acctttggac tttaaaaagt tttaaagtac tgtatgatct ttttagatga
      481 aagtttggat gggggagcat cattggatag tagagtctta aaggaatatt tggataaaaa
      541 tgattttccg attatacgca aaggatatca taaatatttg acattcgaag ggatggaaga
      601 ttcccatacg tatatcttga aagagggaac ggtaaaagca agtgttattt caaatgatgg
      661 gagagaattt aaccttcgct atatcaaaga tcttgaaatc gtctctcttt taaaagacga
      721 atattcacaa ttcatcgatt cgccgtataa tattcgagtt gaatcagaat atgcagagtt
      781 ttatcgaatc aatcgtgtgc gcttttggca aaatatcaac caagacccaa agctacaaca
      841 ctatgtaaaa gagtattacc gctatcgtct gctttattcg atgaaaaaaa tgcagcaaat
      901 gctattaaac ggaaagctgg gagctgtttg tacgcaactt tacgaattat atgatttatt
      961 cggtgtgcgg acagaagaag ggatgctgat cgatttttcg atcacgaatg aagaaatcgc
     1021 caaattttgt ggcatctcaa ctgctagcag tgtaaacaga attttaaaac aactgaaaaa
     1081 agatggtatt atcaccgtta aagaccatca tatcgttatt ttgaatatgg acttgttaga
     1141 agacaatatt atcttttgat gaacaaagag gttgtgaaat cagcgttttg acctgagtat
     1201 tagagaaaca aacggaaaaa tagttcctca tattttgccg ttttttgagc taataccgag
     1261 ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta actctgagtc
     1321 acaaggaaca atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg
     1381 aggagttggt aggagaacac cgtttaccaa gaggttgtga aaaagctgtt ctgcatcaag
     1441 taataagaag agccaagtaa aaacagtctc ttatgttttt aacttggctg gacttatcac
     1501 cgtagatgta agcttttgaa cgccgtttat ttgcaacaaa aggggctgtg acacgatttt
     1561 tgtcacagcc ctttttttgc gaacattcat ttagcaattt ttagaaactt ctgccgccac
     1621 cgccaaaagt cccgccaccg ctagaatggg tagtacttcc gccaccgccg cttccaggag
     1681 gtgggctttt ggggattctt cttgtcgtga caaaagaatt tgtcagacga tcggtttttt
     1741 cagttaattt gatactggat ttttctcgga atggatattt gtagatgcct gatttcaatt
     1801 gataacgcga aacagtcaca aaataaaaga caagactaag gataagagcc aaaacaacag
     1861 caatgatgat ttctactgtc gtcaaaacct tatagcgtgt aattttgcca gtttcttcgt
     1921 ctacacgata atgtcctcct ggaactccgt cttccacata tgttgctgat ttagacaggt
     1981 aagcttttgc agcattgtaa taggcagcat ttgtcatctg atcataaaca tcatctaaga
     2041 tcgaatcgat tctgctatca gtcaaataat cgatcatatt accagaagtc gaaatgtaga
     2101 tttcacgctg tcccatatct aatagcaata ctgagccatt ttgatcattt ccgactgcgt
     2161 ctcttaagta attatcagca aagtctcttg gttcttctga attagatgta gtcgttacga
     2221 tcaagactcg tcctttgatt ttttcattca gcttttctgc ctgttgttcc aaagattgga
     2281 tttgatctgt tgtgaataac ccagcctcat catttaccgt agctgtatct gcatgagtag
     2341 ccaccggcca gccaacactt actagcaaaa gaaagaataa aaaaaatttt ttcatcatat
     2401 caagtaacct ccgatcagaa agaggatcgc tagcccagcg aaaatcccaa gagcggtcag
     2461 tcccaatttt ttctggctga tcggcaggac accgctgacc tttcctgttt gtccattcat
     2521 tgcgtagtag tagacttttt tacttgagtc attgcttcga taagtaacaa gccataccgg
     2581 cagtaatacg taattattta cttcgtttgt gattgaagca gaagttcgca caccggtcaa
     2641 tgttgtatat ccatttgcgg tatcttttaa cagcttctct gaatagtctt ttaattcaga
     2701 ttgaatctgt gatttgattg cctcatattc gatatctctt ttttctgctt ggaacccggc
     2761 taaatactgg cttttaaaat caattgcttt atctaacggg aaaggttgca cgccttcgac
     2821 catcttttgt tgcgtatttt ttgataaggc attttttact agttctttaa aagaaagctt
     2881 gcctgctctt gaaacagcaa attgcttcgt ttctgtgtat tcgatgtcac ctatccgcca
     2941 tatgcggata actgtaccat tcgcctgcat ggcaccatct acttcagcat ctgtagccca
     3001 ataagggaaa tacacacctg tcaacttatc gatttgatcc ttattgaaaa aagctttggg
     3061 aacaaaccat tttttcttgg tccaagcgag aaatctgttg attgcttctt ctttctcgac
     3121 agcaaaagga agtactttat ttggcaagaa ttctccgctt aaacgtcccg atagtacgac
     3181 gggattatgg cagtagtaac aataagtcgc agcagttgtc gcatcagtca cgatctctgc
     3241 accacagttg ggacaaagaa aaagatctat cgctgcggta ttatctgcag attcgtccgc
     3301 tgtatctgaa aaaccgcctg cctcgtcaac cgctttgcgc tcggcttcat tcatatcggc
     3361 aagctgttct tgggctgtaa aagtaaaatc ttcggtaggt gaaggttctt ctttgctttc
     3421 ttcgccagcc atacgcgctt ctttttgctt ttgctcatat tggctgactt cttcttctgt
     3481 atagatattc aggcagtatt cgcaatgaaa tttttggtct ttgggatcaa aagtcaacgg
     3541 accgccgcaa ttaggacatt tatgtgtaag aacatccatc agctgcacac ctttctaaat
     3601 aaattaatta acggggattc ctttaaacgg tttaccacag tttggacaga atttagggat
     3661 gccgttggaa agatccacga tctcattgca ttcactgcat cgcatttgta gcttaggctg
     3721 aacgttttct acaggtttgg cagctccgca gttggaacag aattttccgg aattttctgt
     3781 cccgcaaact ggacatgtcc aagtatccgc atttccttgt gcagtttgtt gattttgttt
     3841 ttctgcttgt tgtttgattt gttcttggtt attttgagct gcttgagaaa gataagaacc
     3901 atttgcattc attcccatac ctacacccat aaagccggtc attgctcctg cttcattttt
     3961 tccggctgct tccataccac gtgcaatgga accttgcacg tagccttcac gtacgctagg
     4021 atcacctagc atcgcccctt cgttacgcat attgatcagt ttcacagaat catcggtgta
     4081 agaaatgctg gctacagcca cagaaacaat ctccatccca cgtaattcac gccaagaatc
     4141 atccaatgca gtatccatgt atttgcttaa ttccaaactc ttagatggta cataagaaat
     4201 ccgttgacca tctgcagaca tctgattgat tgctgattgt agcgctgtta ggaattcagc
     4261 taaatactgc tcattgatat cattgatttc cacttgtgtt ttgtttttag ggattgcgtt
     4321 tgtataaaat aaaatcgggt caacgacatg aatagaataa gtgccatgtg cccgtaagaa
     4381 taattctgcg ttatagaaat tgtcaaagta gttcaacggc gcgcttgtac cgaatttgat
     4441 tccctttatt tcttggagat tgatataaaa aacttgttgt ttctgcggtg tgacgccgcc
     4501 aaatttgaat cggtcaaaag tttcagcaat tgcttctttc agtgttccat tgaacatgga
     4561 aggagctgcg tcatttttaa tggtgtaata accttcctca gcggtataat cgatgatttt
     4621 tccaccatcg accaaaagca tcatcatgtt tggatagaca tggacgactg ttccgtctgt
     4681 taaaacatct tctgttcctt tacgattgga gccacgtttg tcatctttgc ggactttgac
     4741 accttttgtc atgaccgttg tatcactcat attgtctggt tcgatgactt ctagccattg
     4801 atctgccaga ccgccgccaa ccatacttgt tgctgctttg ataagaccca taaaaaattc
     4861 ctcctctgat tcagctagtg ccaaacaatt attgtttatt tattatacca taggaagatg
     4921 tgaaacttct cggactagag acggaattga aatggaaaat tgcgtattgt ttaaatacct
     4981 gaaaaagccg agaaggatag tagttttttt acctaatatc acgtataata agagatagtg
     5041 aattttttaa aaaaggggga tccttatggc atatcaagca ctttatcgag tctggcgttc
     5101 gcagcgtttc gaagacatcg ttggacaaaa agctgtgaca cagacactaa aaaatgctat
     5161 tgtttcccac aaaacctcac atgcctactt gtttacagga cccagaggaa ccggaaagac
     5221 gagtgcagca aagattttcg caaaagcaat caactgtccg aacagtaaag acggtgagcc
     5281 atgcaacgaa tgcgaaatgt gtcgatcaat tactgctggt acgcaagaag atgtgatcga
     5341 aatcgatgct gccagcaata acggagtcga agaaatccga tttatccgtg atcgggcaaa
     5401 ttatgcgcct acaaaggcag cgtacaaagt gtacattatt gatgaggtac atatgctttc
     5461 aacaggagct ttcaatgcct tgttgaaaac attggaagag ccaaaagaaa gtgtcatatt
     5521 catcttagca acgaccgaac ctcacaaaat accagctacg atcatttcac ggactcagcg
     5581 atttgatttt aaacggatca atacggcaga tattgtcgaa catcttgaat ttatcttgaa
     5641 agaatcgact atcagctatg aaccagctgc attgaatgtg attgcccgtt ctgcagaagg
     5701 cgggatgcgg gatgcattga gtattcttga tcaggcaatc tcgtttagtg aaggaacaat
     5761 tactttagat gatgccatgc aagtgacagg tagcttaact gacgaaatga tggacagtta
     5821 cttgagtgct tgcgtgaaaa aagaagtacc agaagcattg gaaacattga accaaattct
     5881 cgcagccggc aaagaatcac gtcgctttct agaggatatg ttacaatact gccgtgactt
     5941 gctgatgtat cagcaagcac ctaacctagt tgaggaacaa tcgagtacga tcagaagtac
     6001 atttacagaa ttggcacaaa ctgtttcagc ggatcagctg ttttcaatga taaagatttt
     6061 gagcgatact caaaatgaaa ttcgttttac caatagtgcc gctatttatt tggaagtcgc
     6121 tacagtcaaa cttgcacagc taccgtctgc ttctgttcct tcagcagatg tagcatttgc
     6181 agatgatcag atagtaaaac agctacaaca agagattaag gaactaagaa acgaaatcaa
     6241 ggaactacgc agtaatacag gaattgagaa tacaaaagta cagccggtac agcaaacaaa
     6301 tcataaaaaa cagacaagta cctatcgtgt accgacggaa cgagtatata aagttctgcg
     6361 ggaagcgaca agacagcatc tgaaccaagt aaaagaagta tgggaagact tgctgatgag
     6421 tctatctgtc actcagcgag caatgcttaa agcaagcgaa ccagttgcag ctggacctac
     6481 aggactagtg attgcgtttg attatgagat tgtttgtcaa agagcagcaa atgacgaaga
     6541 attgcagctt tcggtacaca acaacttaag ccgaatgatc aaagattatg cgcctgatcc
     6601 tgtttatatt acaagagaaa gctggccaga acttcgaaga aattatttga cgcaggcaca
     6661 agaagaggga acagaaaact cttttgcttc tgaagactca gaaggagaaa tcgagttgct
     6721 tcctcaagaa caagaagtac cagaagaaac acttgtagta aaagcagagg aactgtttgg
     6781 ctccgaagct gttacagtgg tagatgatta ataaaaagga gtagataatt atgatgcgtg
     6841 gaatgggcaa tatgcaaggc atgatgaaac aagtacaaaa aatgcaaaaa gaaatggcag
     6901 aagcacaaga gctgctaaat aaaacagagt ttacaggtaa agccaccaat gaattggtca
     6961 aagcggtttt tacaggcgat cgtcgcatga aagatatcca aatcaatgaa gaagtcgttg
     7021 atcctgatga tatcgagatg cttcaggatc ttgtcgtaat ggcagtcaat gatgcgatcg
     7081 cacaaattga tgtggaaact gagaaaaaat tagggaaata cgcaaaaggt ttgccaggat
     7141 tttagtttta aactgaagga gtaatgaata tgcaatatcc agaaccaatt gcgaaactga
     7201 tcgacagcta catgaaactt ccaggaatcg gtcagaaaac agcgacaaga ctcgcctttt
     7261 atacgatcga tatgaaagat gaagtagtaa atgaatttgc taaatctttg ttaagtgtaa
     7321 aacgcgattt gcatttttgt agtatctgcg gaaacatcac agaagaagat ccctgcgaaa
     7381 tatgtagaaa tccttcacgt gatcgaagcg ttatcctagt ggtcgaagag ccgaaagatg
     7441 taatggcaat ggaaaaaatg cgagaatatc acggacttta tcatgttctc catggtgtgt
     7501 tatcaccgat ggaagggaca ggtccagaag acatcaatat ttcttctctg ttgcagcggc
     7561 tgcatgatgc agatgtaaaa gaagtgatta tcgcaacaaa tgcaacaaca gagggagaag
     7621 caacagctat gtatctctca cgtctgatca aacccgcagg tatcaaagtg actcgtctag
     7681 cacatggtct gtctgtaggc tctgatatcg agtatgcaga tgaagttact ttattgaaag
     7741 ctgtcgaagg aagacgtgag ttataaattt ctaattaagt gttttttcag atacaatata
     7801 aaagcagcac aagagatggg ggcaacaatg tgaacggact atttattaca atcgaggggc
     7861 ctgatggtgc aggaaaaacc agcatcttga atgaacttta ccctcttttg aaagaagtag
     7921 caaaaacaga aatcattcaa acaagagaac caggaggcat tccgattgct gaagagatcc
     7981 gagcagtgat ccttgatcca aaaaatgatc ggatggatga acgtacggaa gcactgcttt
     8041 atgcagcagc aagaagacaa catctagtcg aaaaggttct gcctgctctg gcaaaaggga
     8101 aaatcgtttt atgcgataga tttgtcgaca gttctcttgc ttatcaaggt gcaggccgac
     8161 gcatcggcgt taccgagatt gctcggttga atgagtttgc tacagaaggt actacgccgg
     8221 attttaccct ttatcttgac gtagattcag acaccggatt aagaagaatc aagaaaaaca
     8281 gacaaaatca aatcgaccgt ttagattcgg aaggtttgga attccaccag cgtgtacgtc
     8341 atgcttactt aaagctggca gaagagaatc cggaaagaat ccacaaagtt gatgcaagaa
     8401 aaagctttga agaagtgctg caaacaagtt atcatacaat tattgaacag tatccgcaat
     8461 tttttgaaaa ctaggaaggt gaacgtttat gaaaattatt ttagcgatta tccaagacaa
     8521 agacagcaac cgtttatcaa atgaattgat tgatgcaaat atccgtgcca caaaactatc
     8581 atcgacaggc ggcttcctta aggctggaaa cagtacgttt atcgtgggta ttgaagatga
     8641 ccgtgtagaa gaagcattgg aaatcatcaa aaaaacgtgt gaatcaagaa agcaatttgt
     8701 atctacacct gttacactgg atatttcgat ggatgggggc gtcccttatc cagtagaagt
     8761 agaagtaggc ggtgccacag tcttcgtctt acctgttgaa ggattccatc aattttagga
     8821 ggagctgagc tatgaatcaa gaaataaact tggctcaaat gcaaccgatc gtgtatcagc
     8881 agcttcaacg gagttttgag catgggcgta tcgctcatgc ttatcttttt gaaggggaga
     8941 aaggaacagg aaaacatgaa gtaggtatat ggcttgcaca gcacctgttc tgtacccaaa
     9001 tgaaagacaa tctgccttgt ggaaaatgca acaattgtca gcggatcgaa aagaaagaac
     9061 atccagatgt tttggttatt gaaccggaag gacaaaccat caaagtcgat cagatcagac
     9121 gtttgcagac ggaattcagt cgcagcggtt acgaatcaag aaaaaaagtt tttttgatca
     9181 aagaagctga aaaaatgaat agcagtgcgg caaacagttt gttgaaattc ttggaagagc
     9241 cccctggaga ttttttagcg gtgttagaaa cagatgcagt aggaagaatt ctccctacta
     9301 ttcaatctcg ttgtcaagtt ctgcattttc aagaactttc gaaagaagca ctgatccatc
     9361 gattgcaaga agagcagatt ccattggaaa aagcaaaact tttggctttc ttaacaaaca
     9421 gtttaggaaa agcagttgaa atctctcaag atgaatggtt taatgatgct aaggacttga
     9481 tttatcaatg gttcgtttac ttgcaaaagc aagatactca agcctttatc tatgtccaaa
     9541 aaaaattggt aaaatcattt aaagaaaaaa cacaacagtt tacaggatta tctgtcttga
     9601 tgttttatta tcaggaagca cagcgtcaag cagtagcaaa cggcgaatat ggcaaagtga
     9661 aacagataaa ccagataatt gaacggatat tgatcgcaga gcagaaacta cgcagcaatg
     9721 tcagttttca ggctgttgcg gaacagttcg ttttgcatac gattttttag cttgccgcaa
     9781 aggatgatga tcaaggagga tggaaatggt agaagtagta ggtgtccgat atagagatgc
     9841 aggtcatatc tattattttg caccaggaga ctctgaatat gtttatagtg acaaggtatt
     9901 agtcgaatct cagcaatcct tacatattgc tactgttgtc cttcctaaaa aagagattga
     9961 tcccaatgac cttcctgaag ctatccgtcc gatcttgcat aaagcgtcac aagctgaatt
    10021 agaaaaagac aaaaaaaaca aaaaagatgc ttatgatgca ttgaaaatcg caaaagaaaa
    10081 agtgcagttg cgtgagttgg aaatgaaaat ggtcaaagcc gaatacacat ttgatcgaaa
    10141 taaattgatt ttttctttca cagcggacgg gcgaattgac tttagagaat tggtaaaaga
    10201 tctggccgct gtatttcgta ctcgtattga gcttcgacaa attggtgtcc gcgatgaagc
    10261 aaagataata ggcggaattg gtccatgtgg gcgtccatta tgctgttctt cttttttagg
    10321 cgatttcgtg cctgtatcaa tcaaaatggc aaaagatcaa agtttatctt tgaatccaac
    10381 aaaaatttcc ggtttatgcg gacgcttgat gtgctgtctg caatatgaga gcgaagaata
    10441 cgagacagcg aaaaaagaac tccctgactt tggaaaagta attgctacac ctgatggtaa
    10501 agggaaagtc gtagggttga atctgcttag ccgtatcgtt agtattcggc tgactggacg
    10561 ggaaaatcct gtcgaatatg aatgggatga attgaaagaa tttatccaag taggtgagat
    10621 gaatggataa gagatcatta tacgacggat tgaatcaatt agaatcagag ttgagaaata
    10681 cattaacaga acttgttgaa atgaagcatg ctttgcatga gatcgtagaa aaaaatacaa
    10741 ctttggagat ggaaaatcaa cgtctgcggg aacacctgcg ggaattgaac caagttgcac
    10801 aagctccagc tgaaaatgtg aagcaggaac tttctaagtc tcgtatgaat cttgaaaaaa
    10861 tttatgaaga aggattccat gtctgctacg atttatatgg atcaagaagg gacaatgacg
    10921 agccttgtgc gttttgtcta gaagtgatct atcgcgaaag cggtcgatga ccaataacaa
    10981 gaggagactg gagcggagaa atactgctcc agtcttttta ttaaggagat tgtgacagat
    11041 gtataatcaa aaaagtttca agggaaatcc ggtatatggg gatttatatc ttgtaccgac
    11101 gccaattgga aatttagaag atatgagcta cagaagtgtt cgtatgctgc aagaatccga
    11161 tcttatcgca agtgaggata cgcgtaatac acaaaagtta ttgaatcatt ttgaaatcca
    11221 tacaccccaa aaaagtttac atgaacataa ttataaagaa cgtgtccctc aattgattgc
    11281 tttgttaaaa gaaggaaaaa cgattgctca agtcagtgac gcaggaatgc cttccatcag
    11341 cgatcctgga catgagctag ttttagcctg cataaaagaa gggatttctg tgatctctat
    11401 cccaggacct acagctggga tgacggcttt gattgcttca gggctagtgc ctcaaccatt
    11461 tttattttat ggcttccttc cacgtaagaa aaaagaacag aaagaaacat tgaaaaagct
    11521 aaaagaacag acagttacgc tgatttttta tgagtcacct tatcggatca ctgctacatt
    11581 gacgaatttc ttagaaattt ttggaaatcg ccaagtcgtt ttgtgtcggg aattgacaaa
    11641 ggtccatgaa gaatacctta gaggaagtac ggaagagcta ctcgcttata tcgaagaaca
    11701 tccagtaaag ggtgaatgtt gtctgcttgt agaagggaat ttgttcgaaa tgccagtgac
    11761 ggaagttgat gaagagttgg gaacgttaaa ggaacaagta gaagcaaaga tcgcttcagg
    11821 cgaaaaacca aatgctgcta tcaaagcagt agcgctgaaa aatgggctga aaaaacaaga
    11881 agtctaccgt cagtaccatg aattagactg agatagtata agttgtctat aatgaaagac
    11941 taatgaggaa atcagctact aacttttcaa atctcttgaa cgcgaacgaa tgttcttgta
    12001 taatgaataa gaggtgagga tgatggagct gcaatttcct ttacataatg atacatcaag
    12061 aaaaatcatt catattgata tggatgcttt tttcgcttcg gttgaagaac gagatcatcc
    12121 tgagtttcgc ggacatccat tagtaattgc cagacacccc agtgacactg gtgggcgtgg
    12181 agtagtgact acagcaaatt atgaagcaag aaaattcgga attcattcag caatgagtgc
    12241 acaaaaagcg tatgagcttt gtccgcaagc catctttaag cctggcgacc atcataaata
    12301 cagcgatatt tccaaacaag tacgggaagt attctatcgt tatacagata ttgtggaacc
    12361 agttagtatc gatgaagcgt atcttgatgt gaccgaaaat aaaatcaatt gcaagtcagc
    12421 catcaaaatc gcccgaatga tccaagcaga tatttgggag agtgtccagc taacatgttc
    12481 tgcgggagta agttacaata agtttttggc caaattagca tcagactatc aaaaacctag
    12541 aggaatcacg gtagttgccc ctcaagacgc tgtgccattt ttaaaagctt tgccaattga
    12601 aaaatttcat ggggtaggga agaaaactgt acctagaatg catgagctag ggatttatac
    12661 aggaaaagac ctatatgaat gcacagaaat gatgttgatt cgaaattttg gaaaaatggg
    12721 ctattctctt tatcgtaaag tcagaggaat ccatgattct ccagtaaacg ttacaagaga
    12781 gcgaaaatct gtgggaaaag agcatactta tgggcagccg ttgcaaacgg aagaagcagt
    12841 gctgactcag ttgcgccaac tagctgaaaa agtagaagaa gcactgagaa gagtccaaaa
    12901 acatgggaaa acagtagttt tgaaggtcag gtacacggac tattctactg tgaccaaaag
    12961 agtgacattg ccagaatata tctataaaaa agaagcactc ttttatcaag caagccttat
    13021 atgggaggaa atcttgggag tagaaaaagg aatccgattg ttagggatta ctttgacgaa
    13081 tctggacccg atgacttatg aaaacatcgt ccttccatta tgggaaaatc aagaaatata
    13141 aaaacagctt tgagagttga aaccggaaat ccgttttctc tcccaaagct gtttttatat
    13201 caagtcttgc tttttgattt gttcataggt ttctgttgca tccgtacatt tttcccaaat
    13261 cacaacttga ttggattcta aagatttttg gacgtcaatg atccgctggt tgctacttcc
    13321 gcgaaattgt agttttaggt cacgtttgct taattcaaag cgtccgtcaa caaggatatc
    13381 gatttgcgaa aggagttcta atttatcttc tgaatcttgt agcaattcat caaaggtata
    13441 gcctgtccat gaccagatat cttttgtcgt accgaactct tttcggatac gtcgtacgac
    13501 ttgcagacaa acatctgtat tcaaaaaagg ttcgccacca agtaaagtta atccttgcac
    13561 gtaatcgttg cgcaagtcag cgatgatttg ttcttctaat tcaggtgtga atggtttgcc
    13621 atatcgaaaa ttttgtgcag ctttgttgaa gcagccttcg caggcaaata agcagccact
    13681 aacgtaaaga ctgttgcgga caccttcacc atcgacaaaa ttaaacggtt tgtagtcagc
    13741 aatatagttt tggctgtagt cttttgctaa ccattctttt ggttttggat ttctcataaa
    13801 aaaacttctt tcatttaagg ttcaaagata tagctattga agataaaatc tactcgacca
    13861 cataaataag aacaaactga cctaaatcca ttgttcaaga aagcttgttc ttattttgat
    13921 agagggcgtt caaagataca aagccatcga aagagggtgg tgaaaaaaga cttgtcatag
    13981 cctcttattc tgaataaacg gtgttcaaaa gcttgcatct gcagtaataa gcccaactaa
    14041 acgaaaaata tgaggaacta tttttgttta gctattctta ttacttgatg caggacagct
    14101 ttttcacaac ctcatttcat gtgtttcaca cgtgaggaaa tttctttatg gcggccatgt
    14161 accatcggac gagcttgcgg atttcctaaa tagccacaag tacgtttgac tacatcacat
    14221 gtttttggat catgatttcc acattctgga cattcgaacc ctcgttctgt cggtttgaaa
    14281 tcaccttcga aaccacattg atagcaatga tcgattggcg tattcgtacc aagatacccg
    14341 attttgtcat atgcaaaatc ccatacagct tctaaagctt tcggattttg ttggagcata
    14401 gggtattcac agtagtggat gaatccacct gaacaatatt ttggataatc cttctcaaat
    14461 tccaattttt caaatggcgt tgggttttta cgcacatcat agtggaaact gtttgtataa
    14521 tattctttat ctgtgatatt ttcgatgatg ccgaattttt ctgtatccaa acggcagaaa
    14581 cggtcagtta gactttcact tggagttgaa tagacactga aatgataacc atattcatcc
    14641 ccccatgcat ctgcattagc ttttaattct ttgacgatat caagtgtgaa agcttttgct
    14701 tctgggtttg tttcccattc gccaccgaag aaagctgaag caacttcata caatccaatg
    14761 tagcctaacg atacagtagc tcgacggttt ttgaataatt cattgacaga atcggtacgt
    14821 gaaagacgtt tcccgaaagc accatacata tataaaattg gcgcatttgc tggaatggct
    14881 tctttacaac gttctacacg atagagaagc gcatctttca tgatttctaa gcggtcagac
    14941 agtaactgcc agaatttatt ctgatctcct tttgattcga tcgcgattct tggcaaattc
    15001 aatgtaacaa cacctaaatt cattcgtccg acatttactt cttgaccatt ttcgtctttc
    15061 cacccttgca agaatgaacg acagcccata ggtactttga aacttccagt taattcaacg
    15121 attttatcgt aattcagaat atcaggatac attcttttcg ttgcacattc caatgctagt
    15181 tgtttgatgt cgtaattagg atcttcaggg tttaagttca cacctttttt caaagtgaag
    15241 atcagtttag ggaagatagc tgtccgcttt tcactcccta atccattgat tcgaatctgt
    15301 aaaattgctt tttgaatttc tctttcaaac cagttttctc ctagtccaaa tcctaatgaa
    15361 gtgaaaggag tttgtccatt tgaagtaaat aaggtattga tttcatattc caaactttgc
    15421 atagcatcga agatatcttt tttggttttt gcttttgcat attctttttg gcgttctggc
    15481 gaatcgatcc attcttccgc gtcttttaga tgttttttat agttcaattc tgcaaaagga
    15541 gcaagcaatt catctgttcg gtcggcagaa cagccaccat attgacttga cgctacattg
    15601 gcaataatct gtgagatttg tgctgtggcg gtctggatag attttggcga ttcgacttcg
    15661 gcattgccga ttttgaaccc attgttcagc atgcctttga aatcaatcaa gcagcaattc
    15721 gtcatcggtg tataaggatg ataatccaaa tcatggtaat ggatatcgcc tttttgatgc
    15781 gcatttgcta tatgtggtgg caacattttc agtccgattg attttccaac gatcccggct
    15841 gtcagatctc gctgtgtatt gaaaacatta ctgtctttat ttgcgttttc gttcacgaca
    15901 gtttggtcct tgttgattag cttttcaatc gaaaagttga tatctgttgc tttactgcga
    15961 gcaaaatctc gtttggtacg ataattgatg tactcttctg ctaattcata ttcgtttttt
    16021 tctagtaata tatgttccac gatgttttga atctcataga ttttgacatt ttcagcaaac
    16081 cggcttttga tttcttggac aaccaagtct gcaatcattt gaattttttc ataagtaagt
    16141 ggtgataatg agccgtgaat tttcttttct gctttgatca gtgcttcatt gatcttttgt
    16201 tcatcgaaat caacatggcg gccatcgcgt ttgatgactt tcatctggga tgtctctggc
    16261 tgctgagtaa cgacagtttt ttcttcataa tttactttca ttatcttcaa ctcctgctaa
    16321 attgttcttg tctatcataa aacaaaatcg gggaaggagc aactaaatat cggctatata
    16381 tagtattcaa tgtgctgtat aattggattt ctatactata tgtagtgtat attttattgt
    16441 gaaaaaaggt gaaaacttgt agctggtgga tttgtttttt gcatatttaa ttttgctgaa
    16501 taaaataatt gaataaaatt ttttattcag tccaagtaaa agtgacaaaa acataagagc
    16561 aaattattcg tagatatgct actatgcaaa taaatagaaa tgaaaaatga aagaggagga
    16621 gaagaattgg aaaagatgat cgaagtgaaa catctaagta aaagctacgg cagcaaactt
    16681 tctcccgtca aagccttaga taatatctca ttcacagtag aagaaggcga gtttgttggg
    16741 attatgggcc caagtggtgc aggaaagaca acgctgatga atatcttgtc cacgatcaat
    16801 ttgcctacga tgggcagtgt ctcgatccaa ggaaaagaaa tcacaaaaat gaaaaacagt
    16861 gagctcagtg actttcgtcg aaaaaaacta ggattcattt ttcaagagtt caatttgatc
    16921 gataccctaa atgcaaaaga caatatccta ttgcccctag cagttgaacg gatgactaaa
    16981 gaggaaatgg aaaagagagt aagacatgta gctcaattgt tgaatataga agaactgctc
    17041 aaacggtatc ccgatgaact ttctgtcgga cagagacaac gaattgcagc tgctagagca
    17101 cttgttgttc agccacaagt gatttttgca gatgaaccaa ctggctcttt ggactcgaag
    17161 tcggctacag aattgttgaa ctacttgaag accatgaatc aaaaagaaaa agcaacgata
    17221 ctgttagtca cccatgatcc atatacagca agctactgcg atagaatttt atttattaaa
    17281 gatggtgtga ttttttctga agttgtcaga agagggacaa gaagagagtt ctttgagaaa
    17341 gtaatagaca tgcaggcgac gattggcgga ggaggaaaaa tgaatgctgt ataaactagc
    17401 cgttaaaagt ttttttcggc agtcacgagg ctatcttgtt tattttttca gtttgacttt
    17461 atccgtaatg atctattatt ccttcagcgc aatgacctac gatcaatctt tagtcagaag
    17521 agcaagtcaa gatacttcga tagatggtgg attgagtttt ggtggattta tcattactgt
    17581 tgttttgctt tttttcgtat tcagtgccaa ccgatttttc ttgaatagaa ggcaaaagga
    17641 aataggcatc tatcagcttt ttggtatgaa taaactgcag atatcaggta tatatgtgtt
    17701 agaaatcatg ataattggat tgttcgcctg tatttctggg atattactcg gtattatttt
    17761 ttccaaactc ttttcaatga ttttggttcg aatgatggat atggatctta ctagtccatt
    17821 ttttatctcc attccatcag tagtagatac tttatttgct tttttcatca tccttttagc
    17881 agtatctgta tattcaatat ggaaaatatg gcgctatcca atgattcgaa gttttggaga
    17941 aaaagaacaa gcagatagtt caacaatgcg cttcaggaca aggcaccgat tactcgcagt
    18001 catcgggtta ctacttttaa cgagcggata ttttggagct agtcatttta gggaaatcac
    18061 ttcatggatg atcagttatc attatcttga tacattagtt ttcttttttc cttttttaat
    18121 ttttttcgtt tgtgtggtcg gtacttattt gtttttctgt tatacatttc ggttattttt
    18181 gatcatattg agtaaatgga agatttatta ctatggtatc aattttttga taatgggtaa
    18241 cacgcaagtt catttgctca aaagttggcg tacaaattct ctgattacag tcattctcgg
    18301 aatttcttta atgacaattg gcggcacggc tagtatctct actattttat cctaccagga
    18361 gaaagtttcc tccccgatgg actatcagtt tgatgttgag acagagaaaa aaatcaagcc
    18421 gattctagca gaggaagggc agaagataac taaagaaatc accttgcatt ataaagtcat
    18481 tgggagtatc tatagtcaaa atctcgaaga acctatgttt caaactgcta atctgatcac
    18541 ggaaacacaa tatcaagcct tcagaaaagt taatccagat ttgccgaaca tttctttgaa
    18601 atcacctggt catactgttt tgcttgatca aatgcaaact ctttttcgga atatttcatt
    18661 atatggatct agtattggac tgccagataa tcaagaattg aagattcaac aaatgcttcc
    18721 aagtgtatta ggtgacgaaa caatggtata tctaagtcct gtactgattg tagatgatac
    18781 cgtttttgaa aaagcaaaag gtgtggatta tcaagtagtc aatgtagatg tatcaggtgg
    18841 aaataatgaa aaaatagatg aacgactgag caatgacctg tcgataaagt ggctcaatgc
    18901 tgtttattat tcttatgata tcgtaaatgg aagtctacaa ggagaaatta gcacaaagaa
    18961 acttccggat gaccaaatga aggaagcaca atttagccaa gaaagctctc gtttgaattt
    19021 ctcttccagc tattcgaaat tgcgtgcagt taatcggcga atcggtatca atatttttgt
    19081 cacactattt gtcgggatga tcgtgattat tgctactggt agtattttga cagtccggca
    19141 attagcagaa gcagaggaag aaaaagaaaa ttatcaccta ttgactaagc tggggattcc
    19201 acaaagaagg atcagacgga tgatcttcga gcaaaacgca cttaccttct ttccacccat
    19261 gatattaggg atcactcacg cagtatttgc aatcaatgtc ttcactcagt atataaaaac
    19321 tgccgattac tggttggctt atcttgtgag tggcttattg atccttatct atcttttgct
    19381 ttatgtcatt acgagtcagt tatattgtcg gattatcgag gaatgatata cggaataaga
    19441 caagataata aatagaacta aat
//
LOCUS       DACCXM010000049        19042 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCXM010000049 DACCXM010000000
VERSION     DACCXM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.051"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            30..584
                     /locus_tag="IVY61_001471"
     CDS             30..584
                     /locus_tag="IVY61_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7970590.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(757..1230)
                     /locus_tag="IVY61_001472"
     CDS             complement(757..1230)
                     /locus_tag="IVY61_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7970591.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1242..1778
                     /locus_tag="IVY61_001473"
     CDS             1242..1778
                     /locus_tag="IVY61_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7970592.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1818..2189
                     /locus_tag="IVY61_001474"
     CDS             1818..2189
                     /locus_tag="IVY61_001474"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7970593.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2473..3744
                     /locus_tag="IVY61_001475"
     CDS             2473..3744
                     /locus_tag="IVY61_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP7970594.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3989..5014
                     /locus_tag="IVY61_001476"
     CDS             3989..5014
                     /locus_tag="IVY61_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7970595.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5308..6417
                     /locus_tag="IVY61_001477"
     CDS             5308..6417
                     /locus_tag="IVY61_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7970596.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6500..7396
                     /locus_tag="IVY61_001478"
                     /pseudo
     CDS             6500..7396
                     /locus_tag="IVY61_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7412..8236
                     /locus_tag="IVY61_001479"
     CDS             7412..8236
                     /locus_tag="IVY61_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7970597.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8268..9578
                     /locus_tag="IVY61_001480"
     CDS             8268..9578
                     /locus_tag="IVY61_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP7970598.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9726..10985
                     /locus_tag="IVY61_001481"
     CDS             9726..10985
                     /locus_tag="IVY61_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7970599.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10995..11867
                     /locus_tag="IVY61_001482"
     CDS             10995..11867
                     /locus_tag="IVY61_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7970600.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11879..12709
                     /locus_tag="IVY61_001483"
     CDS             11879..12709
                     /locus_tag="IVY61_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7970601.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12749..14932
                     /locus_tag="IVY61_001484"
     CDS             12749..14932
                     /locus_tag="IVY61_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7970602.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14993..16213
                     /locus_tag="IVY61_001485"
     CDS             14993..16213
                     /locus_tag="IVY61_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP7970603.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16210..17670
                     /gene="gtfA"
                     /locus_tag="IVY61_001486"
     CDS             16210..17670
                     /gene="gtfA"
                     /locus_tag="IVY61_001486"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP7970604.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17747..18853
                     /locus_tag="IVY61_001487"
                     /pseudo
     CDS             17747..18853
                     /locus_tag="IVY61_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         6462 a         2906 c         3715 g         5959 t
ORIGIN      
        1 tcagaatatt tttaagaagg gaaacaatag tgagctataa tgtacaaccg ttaaggacaa
       61 atgaagagat acaagatttt cagttttggc taagaagaac aagtaatccg gaaagagata
      121 gttttttatt tttgtttgga atcaataatg ggttacgtat gtcagatatt ataggattaa
      181 aagttggaga tatacgaggc aaaagtaagc ctattattgt tgaacataaa acaggtaagc
      241 gaaaaccgat ttttatagat aatttgagag aagaaatttt attatatact gaaggtaaag
      301 aagaaaatga ttggttattt ccgagtcggc aacaaggtcg acatataaca agggatcgtg
      361 tctatcagat atatgcagat atagcagaaa aattaggacg agatgatata ggaacacata
      421 cattaagaaa aacttttggg tatcactact acaagaaaac tagagacatt gctaccttaa
      481 tgtttatttt taatcatagc tcgcaagcaa ttacaaaaag gtacattggt ataactgaag
      541 atgaaatagg agcatcactt cgtggattta aactaggtgt ataatttaaa aatttttata
      601 aacagtaaag acaaagatta aaagctattt ttatctagaa cgattttatt tcataaagtt
      661 cgcggaataa aagctggggt tactggtact tggtataggt tcgtgttata taaactaagt
      721 aattaaataa catttttagg ttggaaaagg atgaaattaa aaaaagagaa tcaatacggt
      781 ctcaaaaacg caaacgctgc ggaataagaa aaatactgat aactcaacgt ttagcagact
      841 atctaatagg gatagtctgc ttttttcgtt tttgaccata cttttgacct tctgcaaact
      901 tccttgcttt tcctctgtag aactcatgaa atctgcaaaa cgaattgccg tttcctctgt
      961 ctgctttttc gcgatatggg tgtatatatc atccatagtg gttttaatat ctgtatgtcc
     1021 aagtcgaaca tacacttctt tgacagacgc accagattca aagagcagac tacaatgggt
     1081 atgacgaaaa ccatgaagtg tgatatgttt taagttattc tctttaatga tgattttcaa
     1141 attgtgattt acatagtcta aataaagggg cttattttct tctgatgaaa acaaaagttg
     1201 ttcaccatct tttataggtt tgattctcat ttttagatat tatgcccgca cgtattcatc
     1261 acatttctat tgtgaatcgc tttattcgac cgacatttga cttttatcat aatatactcg
     1321 gtttaaaatt actaatgaaa acaattaatc aagacgatca taccatgtat cacctgtttt
     1381 tttcagataa tcaccaccgt gtgggaactg aactgacttt ttttgaagta caagaaggga
     1441 acaaccagtt ttttggaacc aatacgattg aacgaacgat actaaaagtt ccttccgaag
     1501 catcattgca tttttgggaa atatattttg aaacacaagg cgtttgccat tatggaatcg
     1561 aatcattttc tggacgacct atcttacgtt ttgaagggcc agatgcaaca cgattagctt
     1621 tagttccctt acgcgaattt gaagatattg atgattattt cccgtatgtc acagaccaga
     1681 tccctacaga gcatgccatt ttaggaattg atgccattca actacgcgta caatattccg
     1741 atgcgactga acgagagtta ttgtcggtag gctggtagca caaagaatac gtcagtttct
     1801 tttcgggtca tcaacgagtg acaatcttag aaaatcaaga cactcagttc tatcaagaag
     1861 ttcagattat tcaagatcgt gagaatccag ttgccattga agggattggt agcgtccatc
     1921 atgttgcgtt tggcgtagaa aataaatctg atttacaacg catcgacaaa caattacaag
     1981 agcgcaattt tataaattca ggaataaaag atcgtgaatt ttttgtatca ctttattatc
     2041 gtgacccaaa tcaattattg attgaaattg ctacagggga aggaaaccta gatgcaaaag
     2101 cgtatgaaaa tcaaagtcca cgctttgaag aaattccttt atttttaccg cagtacttaa
     2161 atttcattcg tgaacaagtg gagcaataat tagctaatca aatccatgac gaagagtaag
     2221 aggttgactc ataagtcaaa aaataaagaa aaacgcatca aatccattct aaagaatgtt
     2281 gatttgatgc ggttcctatt tttataaact atcattttcg actaataaaa ctacttatga
     2341 gtcagcctct ctttttttct tgatttttat acctctttct aggtaaacta aacatctgcc
     2401 aaaaataccg tgtctcattg aaacattctc aactatttca aaataaaggg gagtaccaaa
     2461 tgaacgagta tattgaaatt atccacgcaa gagaaacaac ttaaaaatgt cagtctcaaa
     2521 attcccaaaa ataaaattag tatttttacc agcgtatctg gttcaggaaa atcatcgatt
     2581 gtttttgata ctattaccca agaagctggt cgtcaactga atgacaccta tgatagtttt
     2641 acccgtttat ttttacctaa atacagtcgt ccagaggttg atgagattga taatctttct
     2701 acggcgattg tggtcaatca aaagaagatt ggtggcagtg cgcgctcaac gttagggacg
     2761 attactaaca tcaatgcgtt atttcgtgtg ttgttttcat gttttgcaac acctagttta
     2821 ggatatgcaa atgcttattc atttaatgat cctagtggaa tgtgttctgt ctgtcaggga
     2881 atcggtaaga cgattgcatt tttaatagga ttgctttatt tttcggctat tgataatgct
     2941 gattttgtat tttctgatcc atttaccgca gaagttttgt ctggaattga ggaacaggca
     3001 aagaatcatg gttattttgt attggtgcat aatgttaatt cagcagaaga tgttaaaact
     3061 attcagagaa attggcgttt tgaggggttc attgttgttg gtgtacgtgt aaaagaattt
     3121 ttggaattaa ataacgtttt tgatggtcct gttgtttata ttgatacgca tctttcaaaa
     3181 gagacattaa gagaacttga acaacaaatg aatcgtttat ttgtaaatac aaatgattac
     3241 caagctagta cattggcagt ggaacatttg attcattatg gtcatgagaa aatagcgttt
     3301 ctttcttttg actacattgt aggtgaacca agtgttattc aagaacgaca tcaagcttat
     3361 atcgatacat taaagaaata tcatttaacc tttaaatcgc attggatata taacaactct
     3421 gaatttgagc gaatttatca agaattagct gaatttactg caattgttgt tactggagat
     3481 tatttagcgg ctaaattagt aaaattttta aaaaataaga ataaacgagt gatggaacaa
     3541 ttatcgatta ttggatttga tgatattact tttgcagaat taatggatcc tgcgttgaca
     3601 acaatacgac taaatccagg taacaagggg gcgattgctt ttcaccaact gattggactg
     3661 gccaaacaag aagaaataaa aaatcaagta ctactattag agggagaatt gatttctcga
     3721 gactctgttc acaaaatcaa ataaaaaagc tctgtgaaat aagtatagtt aacaataaaa
     3781 tatccgaacg atagagagaa tgaactgtga tctagcagtt cctcgtcgca aattcactag
     3841 aattagcatt atttactcta aattgttcgg atattttttc ttgtttttaa ttatgattac
     3901 agtgcctatt tttaacgaaa tttgcttatt ttcaaaatgt atgattatat gttagtaaac
     3961 actttcaaat atgaaaagag gagctattat ggtaggaatt agggatgttg caaaacgggc
     4021 gggtgtgtcc attgcaacag tatcgcgcgt gctaaatgag gatccgacac tttcagtaac
     4081 cgatgaaacc aaacgaaaga ttgcagaatc ggttaatttt tatcattatg tcaaaaaaag
     4141 tcccgttgca aaaaatttaa caaatattgc attaataacg acagtttctg aagtagatga
     4201 gttggaagat ccctattttc gaacgattcg cagagggatt caagtcgaag cagaacaaag
     4261 caaagtcaat ttgaagaaaa ttattcgttt gtcagaaaca ccgttggagc aagacgattt
     4321 gctaaaatgt caaggtgtgt tgattattgg gcaggtgcta tcttctgtga ttgaagaagt
     4381 atcagcaatt aattcgaatg tcgtagtgat tgatgaccct agtattgaag ctgaagtaga
     4441 tgcagtttat actgatttaa caaaagcaac gaatcagcat ttagatcgat tgtataaaaa
     4501 aggacaccga aacattgcgt ttatcggagg aggacgtgtt cgtttagata gccaaggaaa
     4561 taaacacgtg tcagatgatg attatcgaaa aatagcctat gagacgtgga tgaaacaaaa
     4621 aggattagaa aaatatagtc aaatttattt agaaggctgg accacacttg acggaatgca
     4681 ggcagcagat ttacttttga aaaaacatgc taaagataag ttgccgactg ctatcatggt
     4741 aggaaatgat cctattgctg tgggggttta tcgttcactt caaaaaaatg gtctacatat
     4801 tccagaagat atttcgattg tgagttttga taatattgaa gttgctgaat ttttaacacc
     4861 tgcattaaat acggtgaatg tgaatacaga agaaattgga aaactagcag ttcgcatggt
     4921 taatgaacga ataaaagaag ttagaaatgt ttcgatcaaa gtgattgttt ctaataatat
     4981 tattgttaga gaaagtgaaa aaaatatcga ttaaaaaagt aaacatccaa aaaagggtgt
     5041 ttactttttt gtttactaat atttttagta aaaaatctcg acaaaacatt aagtaaacag
     5101 ttatggtgtt gttaacaaaa agaaaacgca ttcaaaacag gaggaattaa gatgaaacat
     5161 ttcaaaaaaa tagctttaac agggatagct gttttaactg ggacattact cgcagcatgt
     5221 ggtgggggag atagtaattc taaagacggg caagtaactt taagctatgc gagttgagat
     5281 actgaccaag ctccaggact aagaaaaatg ttagatgaat ttgaaaaaca aaatcctgat
     5341 atcaaagtag aaatggagac aacgccttgg gatcaatatt gggttaaatt agaagctgca
     5401 acaacgggag gtaatatgcc agatgtagta accatgcact ctagcgaatc ctataaatat
     5461 atgtcacaga atgctttgat gggacttgat gatgttatca aagaaaatga tattgacatg
     5521 ggaaacttta ctgaaggaat tgctgatttc tatacgcttg aagacgaact atacgcaatc
     5581 cctaaagatg cttctgtagt ggggctatgg tacaacaaag aattatttga taatgcaggt
     5641 gtcgattatc cagatgaaac atggacatgg gaaacactca gacaagctgc aattgattta
     5701 acagatgaat caaaaggcat ctatggtttt gcatcacata ttggaacaga gactggctac
     5761 tggccattta tttatcaaaa tgggggagaa gtatttgccg atgacaatac aaaatcaggt
     5821 ttgaataccc cagaagcaca agaagcttta gctttttatc gagatattgc acttgttgat
     5881 catgcttcac caacaattac agaattacaa gatcctgata agttaagtcg ttttcaatca
     5941 ggtagattag caatgatgat tgaaggaaac tggtttgcac cgggattttt gaaaaatgac
     6001 tatctagttg aaaatgttgc ttctgccgtg ttacctaagg gaaaagttaa tgcaacaatt
     6061 acaaatggac ttggttgggc tgccagtgcg agtacaaaat acccagaaga agtgaaaaaa
     6121 ctaatggcat ttttagctag tgaggacgcc aataaaatcc aagcggaaac gggggcatca
     6181 atcccagcac taaaaggctt tgaagacgag tgggctgctt cttcagatac aattgatttg
     6241 agtgcttttg gaaaaatgtt ggagtatgga gtccctcgtc catacaataa agatggatta
     6301 cgagctgaac aagcagaagt tcaaattatt aaccgtattc tgtctggtga agtaactgtt
     6361 gaagatggaa ccgctcaaat tgaaaaagaa gtcaatgcga tattgagcgg aaattaaatt
     6421 agttaattat acaatataca ggcgaaagga ttctatttcg tctgtatatt aatggatgaa
     6481 taccaaaaag ggggatatta tgaaaaatcg aatttttgga aaatatcgta aagatcaagt
     6541 gatatggggc tatattttta tttttccaac ggcattagga ttaattttat taaatatatg
     6601 gccagcaatt caaacagcaa ttttaagttt tcaaaaaacg gtagggttcg gtgatactga
     6661 atgggtcgga ttaagaaatt accaaaaaat gtttgcagac ggtgaagttt ttcagtcttt
     6721 attaaacaca ttaatttatg cagtggtttc gatacctagt attgttgtat tatccttgtt
     6781 ggtggcagtt ttaatgaata gaaaagttaa aggattgtcg atttatcgga caatttattt
     6841 tctaccagtc gttgctgcac catccgcagt cgcaatgatt tggcgctgga tgttcaataa
     6901 tgattatgga ctgattaata acatgttggc taaaataggt ctcgatggac cagcatggtt
     6961 aacggatcca tcgattgcga tttattctat tatcattgta ggagtttgga gtgctattgg
     7021 ttataacatg gtgttattac tagccggctt ataagaaatt cctaaagatt attatgaagc
     7081 agcggaaatt gatggtgcta gtccaatcca gcagtttttc aacattacgt taccattagt
     7141 tacaccaaca ttattttttg tagttgtaac aacagttatc aatgctttcc aagtatttga
     7201 tgttattttt atgatgatta ctcctaatag taccgccatg tttaaatcac aatcattagc
     7261 ctatttattc tataaacatt catttatttt aaatgacaag ggatatggtt cggcaatcgt
     7321 tatgttcctt ttagtgatta tattaattat tactgcaatt caaatgaagt tgcagaaaaa
     7381 gtgggtaaac tattaaggag gctatcaaat tatgaaaaat aaattttttt caaatgcgcc
     7441 aatccatatc atgcttattt taggtggtat tgcaatggca ttacctttta tttggatgat
     7501 tttaacttca gggaaaacgt tggttgaatc tacccaaatt ccacctacaa ttttccctga
     7561 gaaatttcag ctaagtaact atcaacaagt acttgattta ttaccattca tggcttttta
     7621 tatcaataca tttttaatgg ttgttggacg agtgattggc tcggttttat tttcagcaat
     7681 ggctgcatat gcttgtgcgc gtctaagttt tccagggaaa aatatccttt tcagcttagt
     7741 acttattcaa atgatgattc ccggttccat ttttattact ccacagtatt tattagcaca
     7801 aaaattgggt attttaaata caattccagc attaatatta ccaggggtag ttagtgcgtt
     7861 tggtacgttt ttattaagac aatttttctt aggtttacct gatgaattgg aggaagcggc
     7921 ctatttagaa ggtgccaata tttggacgat ctttttcaaa attatgttgc cgcttgctaa
     7981 atcaggattg gttgcacttg gaatattcac agcgttgttt gcatttaaag atttaatgtg
     8041 gccattgatt gtcaatactt caattgataa gatgccttta gctccgggat tagcaaactt
     8101 acaaggacaa tatgccacga acttcccaca attaatggca ggttcgttat tagcaatttg
     8161 gccaatagtt gtgatattca tcgttttcca aagaaaattc attgaaggaa ttgctacaac
     8221 aggtagtaaa ttataaaact attaattagt gattggagag taagtacatg acaaaaatta
     8281 catttattgg tgcaggaagt actatttttg caaaaaacgt attaggcgat gcgatgttaa
     8341 ctccaagtct acaagatgca catattgcat tgtacgatat tgatgaaaaa cgactaaaag
     8401 aatcaaattt aatgcttaaa aatattaata aaaattcgaa tgaaaatcgt gctgatattg
     8461 tttcttatta tgatcgcaaa gaagcgttaa gagaggctga ttttgttatc aatgctattc
     8521 aagtgggtgg ttataaacct tcaacagtga ttgattttga tattcctaaa aaatatgggt
     8581 tgcgacaaac tattggtgac accatgggga ttggtgggat ttttagaacc ctacggacac
     8641 taccagtaat gttagacttt gctaaagaaa tggaagaagt ttgtccaaat gcttggttat
     8701 tgaactatac aaatccgatg gcgatgttaa ccatgggtgt tttaaaagcg acgagtatta
     8761 agactgttgg tttatgtcat agcgttcaag tatgcgtacc agaattgttt gagcatttag
     8821 gaatagccga tcaatatgac ttaaatgact ttcaatggaa aattgcaggc attaatcata
     8881 tggcattctt gttggaaatt acaaaaaatg gggaagattt ctatccaaca attcgtcagt
     8941 tagctgcaga aaaaccaaat ccacatccag attctgtacg ttttgaattg ttgaaacgtt
     9001 ttggttacta tattacagaa tcaagtgaac acaatgcaga ataccatcca tactttatta
     9061 aatcaaaata cccagaattg attgacgaat tagggattcc gttggatgaa tacttacgtc
     9121 gttgtgaaca acaaattaat gattgggaaa gcatgcgaga ggacattgta aataatgggg
     9181 agttaacaca tacgagaagc cgtgaatatg cttcatatat catggattct attacgacag
     9241 gaactccgtc agtgattgca ggaaatgtct taaataaagg cttaatcaca aacttaccga
     9301 cagatgtttg tgtggaagta ccttgtttag tcgataaaaa tggcgtgcaa ccaacctatg
     9361 tcggtgaatt gccaacacaa ttagctgctt taaatcggac gaatatcaat gttcaagaac
     9421 tgacagtaga agccgcttta acactagata aatcaaaaat ttatcaagca gcgtatttag
     9481 atccacatct atcatctgaa ttgtctttaa cagatatcaa aaatttagtg gatgatttaa
     9541 tcgaagcaca tggggactat ttacctgaat ataattaaaa agaagctgaa agagtaagga
     9601 attttcaatt ccttactctt ttcttagtta aatttagtaa aaatattgac aaaacactta
     9661 gtaaacagtt acattagtgt taacaaaaag aaaacgcatt caaattgtat aaggaggagc
     9721 ataaaatgaa gtggtctaaa aaaattattt tagcttgtat agcactagga gcaacgatct
     9781 ctttgactgc ttgtggaaaa aatcaggaag atgcaagcgg taaggtagaa atagagtttt
     9841 ttaatcaaaa aaaagaaatg actcaaacga ttcaggagat tgctaaagat tttgaagcaa
     9901 aaaaccctga tattcatgta aaagttgtgg atgtaccgaa tgctggtgaa gtaattaaaa
     9961 cacgtatgct tgcaggcgat gtaccagatg tcattaatct ttatcctcaa agtatagaat
    10021 tgaaagaatg ggcgaaagct ggttatttag aggacttaac cgaagaaccg tatttagaga
    10081 atatcaaaaa tggatatgca caacgttttg ctattgagga tcgtgtatat agcataccat
    10141 taaccgctaa tgtctatggt ttttactata acaaaactgc ttttgaggaa atggggattc
    10201 aagcaccaga aacttgggca gaatttgaaa aaatagtagc tgagattaaa gatcaaaaca
    10261 aagtaccgtt tgcgattgca ggagcagaag gctggacttt aaatggttat caccaactag
    10321 ctatagcgac tgctgctggt ggtgaagagg aagcgaacaa tgtttggcgt ttctcagaag
    10381 ttaatggaat taatgctgaa agcaaagaaa tgcaaaaaga ttttaatcga ttagatttgt
    10441 taagagaacc aaaagctttg caaaacaact ggcaaggtgc cggttataac gatacagtag
    10501 ttacttttac aaaaggtgat gcgttgatta tgccgaatgg ttcatgggca atgccgatga
    10561 ttcattcaca aaatccagat tttgaagtag gaacttttcc ttttccagca gatgaagcag
    10621 gtcaaagtct aacgattggc gcaggagacc tagccttatc agtttctgca acttctcaac
    10681 ataaagaaga agccaaacgt tttgtagaat atatgacaac acctgaagcg atgcagaaat
    10741 attatgatgt agatggtgca ccgtgtgctg ttgagggcgt tatcgagaat actcaggatt
    10801 caccattaag tggtttaact gaacttgctt ttactgatcg tcatttagtt tggttagcca
    10861 aagattggaa tagtgaaaat gatttttata ccttaacaac aaattattta cacaatggca
    10921 atcaaaaagc catgattaac gcattaaatg ctttctttaa tccaatgaaa gctgacggaa
    10981 ggtaggaaaa taaaatgaaa ataaaagaaa ttttaaatcg ttactgggca atcatttttg
    11041 tagcaattcc aattattttg cagatgattt ttttctactt tccaatggtt caaggtgctt
    11101 tttacagttt aactaattgg actggtctga cttataattt tgattttgtg ggtttgaaca
    11161 attataagat tcttttttct gatccgaagt ttatcaaatc gattggcttc actttaattg
    11221 tgacgctttg catgattgct ggacaaattg gttttggtat tttgattgcg cgggcgttga
    11281 acgcaaatat taaagggaaa acattcttta gagcatggtt cttttttcca gcggtcttat
    11341 cgggcgtggt tgtttcttta gtatttaaac aaatttttaa ctatggttta ccaaaaattg
    11401 gtagtatgtt aggcattgaa tttttacaaa ccagtctttt aggaacaaca tggggagcga
    11461 tttttgcagt aatttttgtt ttactttggc aaggaattgc catgccagtc atcattttct
    11521 tagcgggttt tcaaagtatt ccacaagaaa ttattgaagc tgctagaatg gatggggcga
    11581 atgataagca aattttttgg aatattgaaa tcacctactt attaccaagt atttcaatgg
    11641 tatttattat ggcattaaaa ggtggcttaa cagcatttga tcaaattttt gctttaactg
    11701 gtggtggacc aagcgatgcc acaacatctt tggggttact tgtttataat tatgctttta
    11761 aaaacaatca gtttggttat gcgaatgcta ttgctttagt cttatttatc ttaattggga
    11821 ttgtttcact ggcacaatta aaattatcga aaaaatttga aatctaggag gtttcccaat
    11881 gaaaacatca aaagaaaaaa atatttctaa atacttgctt ttattattag ggggcttcat
    11941 gattttagtt cccttagttt caacgctatt cggttcattt aaaacaacac ccgatattat
    12001 gaataatttc tttcgttttc ctaatccagc gactttggaa aatttccgaa gattgttagc
    12061 ggatggaatt ggtcattact tttggaattc ggcaatcatc acaatcattg cagtagttgc
    12121 tgtgacagca attattccta tggctgctta ttcactagca agaaaaatga ctcgtgggaa
    12181 agcatacgga ttgatgtatt tatttttaat tttaggaatt tttgttcctt tccaagtaat
    12241 tatgattccg attaccgtta tgatgagtaa aattggttta gcaaatatgt ggggattaat
    12301 cttactctat ctcacttatg ccgtaccaca gacactattt ttatatgttg gttttattaa
    12361 actaaatgtg cctatcagtc tggatgaggc tgctgaaatt gatggggcta gtcactttac
    12421 aacttatttc aaaattgttt tcccaatgtt aaaaccaatg catgcgacga ctattattct
    12481 taatgctcta tggttttgga atgatttcat gctaccatta ttaattttaa atcgtgattc
    12541 taatatgtgg acactaccat tgtttcaata taattatacg ggacaatatt ttaacgacta
    12601 tggtccaagt tttgcttcct atacagtagg aattgtcacg attacactag tttatcttgc
    12661 tttccaaaga agtattatag ctggaatgag caatggagca gtgaaataac caataaatta
    12721 ttctcattat aaaggagttc ataaacttat gattatattt gacaatcaca cacaaacatt
    12781 tcatttacaa aataaaaaaa ttagctatct attaggaatc gaagaaaata attatttaac
    12841 gcatctttat tttggaaaaa agattacgca ctattctggt gggtatcgct acccccgaac
    12901 agatcgttcc ttctcaccca acaatgcaaa tgcgcaagat cgtttattat ctttagacac
    12961 attaccatta gaatttccag gatatggaca tggtgatttt agagaaccgg cgcataatat
    13021 taaattagga aacggtagtc gtgtaaatga ttttcgttat gatagctacg aaattcaaaa
    13081 aggaaagccc agcttagcgg gattgccagc cttatatacg gaagatgatg atgaagctga
    13141 aacgctgatt attcaattga aagacgcgat tagtggattg actttacagt tgttctacac
    13201 aatttatcaa aatcacagtg taattattcg ttctacgcaa ttagtaaata atagtgatga
    13261 aacagtggag attaaccgct tagctagtca atcaattgat tttccaaatc gtccactcga
    13321 gttaattcat ttaaatggta cctgggcaag agaacgtcaa atgactagag aaaaaattca
    13381 tacagggata aaagtattag atagtaaacg cggatcaagc agccatcaac aaaatccgtt
    13441 tgttgcttta gtagatgcca aaacagatga atttcaagga gaggcatatg gatttaattt
    13501 agtttatagc ggtaatcacg aaacggtgat tcaaaaagat ccatttgacc aaacacgggt
    13561 cattatggga attaattcat ttaattttgg ttggcaacta aaaactggcg actcatttca
    13621 aacgccagaa gtcgtgatgg tttatagtaa tgaaggtctg aatacgttat cacagacgta
    13681 tcatgaattg tataatcacc gtttaattcg tggaaactat cgtttaaaag aacgtcccac
    13741 gttaatcaac aactgggaag caacgtattt tgattttaac gaagaaaaaa tcatgaatat
    13801 tgtcgatgat gcgaagaact tgggcattga aatgtttgtt ttagatgatg gttggtttgg
    13861 cggtcgtgaa aatgacttca cttcattagg tgattgggtt gaaacagaag gcaaacttgc
    13921 ttgtggtttg gaaaatttag ccaagcaagt gcatgataac gggatgaaat ttgggatttg
    13981 gtttgaaccg gaaatgattt ctgaagatag tgaactattt cgagcacatc cagattgggc
    14041 attgggtatt ccaggtcgtg accgctcaat tagtcgaaat caatatgttc ttgatttttc
    14101 aagacaagat gttcgtgata atatctatca acaaatgacg gcgatttttg atcgtgtacc
    14161 gattgattat gtgaagtggg atatgaatcg aaatatgaca gaagtctatt ctgtcttgtt
    14221 ggactcaacg atgcaaggtg aagtggctca tcgttatatg ttagggttat atgaattttt
    14281 agaaaagtta acaacgaatt atccaactat tttatttgaa agttgttcgg gtggaggcgg
    14341 tcgttttgat gcaggtttct tgtattacat gccacaaact tggacgagcg acaataccga
    14401 tgccgtggct cgtctgaaaa ttcaatatgg aaccagctta gtgtatccaa tttcaagtat
    14461 gggtgcgcat gtttcagcag tcccaaatca tcaaacagga agagaaacga gtttaactat
    14521 tcgtggcaat gctgcgatgg ctggtgtttt tggttatgag ctagatctaa cgcaattaac
    14581 ttctgaagaa aaagcagaaa ttaaagaaca agttgccttt tataaaaagc atcgccaact
    14641 tttgcaatat ggaacttttt atcgcttgga aagtccgttt gaaggcaatg atactgcatg
    14701 gatgtttgtc tcaaaagacc aaacagaagc catggtcttt tatttccgag tattagcaga
    14761 agctgcgcat ccattagtca cattgaaatt agcaggatta gcagaagaag caacttatca
    14821 aatggaagat gtaacatttg ctggcgatga attgatgaat ctcggttttt atattgactc
    14881 agaactacat ggtgattatg cgacacaacg cttttatttg aaacagatat agtagcttga
    14941 atttacaata taagtgcaac accaaaagag actcattcca aacgaagaaa atatgtttta
    15001 tttacacgca tttactaaaa agcagcctga tttgaattgg gaaaatgaag aagtacgtga
    15061 agaaatctat gcaatgatta actattggtt agataaagga ctaggcggat ttagaatcga
    15121 tgcgatatta aacatcaaaa aaaaactaga gtatggcatc tttgaatctg atggagaaga
    15181 tgggttagcc ttcattgggc cctggattct taatcagtcg ggcattgaag tgtggttaaa
    15241 agaaatgaga gagcggacat ttaaaccaca taatagtatg acggtagcag aagccgatgt
    15301 tcctaatgaa cgtttagatg aatatattgg tgaagatggc tattattcga tggtttttga
    15361 ttttagttat acggatattg atgtacctga aactggtgaa tggtttaaag atagccaatg
    15421 gacatggacg gatatgcgta ccaatatttt tacgaatcaa ttagttacgc aagataaagg
    15481 ttggggcgcc ttatatttag aaaaccacga tcaaccacgt tcaattaata aatatattcc
    15541 agaagaatgg atcaatgatt atagtaaaaa aatgttggcg acgcttttta tgttactaag
    15601 aggtacccct tttatttatc aaggacaaga actaggaatg accaacatag aaatggcatc
    15661 attagaagat tttgatgatg tagctacgca ttctcagtat aaacgggcat tagaatatgg
    15721 attggcacca gagcaagcat tgaaagctgt caataaacga agtcgggata attctcgcac
    15781 acctatgcaa tggaatacgc aaaaaaatgc cagattctca acttctgata atgtatggtt
    15841 aaaagtaaat cctaattatc cagaactcaa tgcagaagct cagttaacca atcaacagtc
    15901 ggtattcaat tattatcatc aattaattaa attaagaaaa aatagtgtat acaaagacgt
    15961 gctaatctac ggacaatttc taccagtgaa agataaaaat gaacatattt tgctttatga
    16021 acgccaatta gaagataaaa tcgtattagt tatcttaaac gttacagcaa cagaacaaga
    16081 atattatgta gatgaacagt accaacaagt attaatcaat aattatgaag atgttttatt
    16141 agatcaaagt cgaaaattgc gtttacgacc gtttgaaagt attgtattag ctaattatgg
    16201 aggacaatta tgaaaattaa aaacgaagca atgctcatca cctatgcaga tagtttagga
    16261 aaaaacatgg gcgaattaaa acaagtctta gacaaacatt tacaaggtgt tattggtggt
    16321 gtacatctgt tgcctttttt tccatcgaca ggtgatcgag gctttgcgcc gagtgattat
    16381 acgacagttg atccagctct tggaacatgg gaagatgtgg aagcattagg tgaagattat
    16441 tatttgatgt ttgattttat gattaatcac atttctcgtg aatcaaaatt tttccaagat
    16501 ttcaaaaaaa accatgagca gtcaccatac aaagaaatgt ttatccgtat tcatgaattt
    16561 ttcccagaaa atcgaccaac acaagaagat attgatttaa tctacaagcg taaagacaaa
    16621 gcgccgtttc aaacggtaga atttgcggat ggtacgacag aagaggtttg gaataccttt
    16681 ggggaagaac aaattgattt agatgtgacc aaagaagttg tgaaagaatt tattcgagaa
    16741 accatcaaag atatggcgaa ccatggctgt tctttaattc gattagatgc gtttgcttat
    16801 gcgattaaaa aattagatac caatgatttc ttcgtggaac cagatatttg ggaactttta
    16861 gacgaagtgc gtcaagaagc agcgaaatat gacgtcgaat tattaccaga aatccatgag
    16921 cattattcca ttcaaatgaa aattgccaat catgattatt atatctatga cttcgccttg
    16981 ccaatggtca ctctgtattc actttatagt ggaaaatcag aacgtttagc caattggttg
    17041 aaaatgagtc cgatgaagca gtttacaaca ttggatacgc atgatggaat tggtgttgtg
    17101 gacgctcgtg atttattaac agatgaagaa ttggattaca cctctgagga attgtataaa
    17161 gtcggggcga atgtgaagaa ggtctattca agtgccaatt acaataattt agatatttat
    17221 caaattaaca gcacctatta tagtgcatta ggcgataatg accgtagcta tctgttagca
    17281 cgtgcgattc aagtatttgc gccagggatt ccacaaattt attacgttgg gttattagca
    17341 ggaaaaaatg atattgaact gttggaagca acgaaagaag ggcgtaatat taatcgtcat
    17401 tactatgatt tagccgaaat tgaagaagaa gtgcaacggc cagtgattca acaattattt
    17461 gcacttttaa aatttagaaa tgaatctgcg gcgtttgatt tggatggaac gattgaagta
    17521 gcaacgccat cagaatcaga aatagtgatt acacgtaaaa ataaaaacgg tagtgatagt
    17581 gcaacattaa cggctaactt ggatacgaaa gaatttatca ttgagtctaa agatataaaa
    17641 attttcacta gtgttgcatt aaaattttaa cctattccat agaacaaaaa atcctttata
    17701 ctagcacttg gatgacttat ccaagaccaa taaaaaggac tcatacatgg ataagtataa
    17761 aacaaataca gcatttaata aatggttttc tgccataaaa ttaggaaaac tttctgtcaa
    17821 ctcccaaaaa tgtatttttg attacaattc gtatcgtaaa aaactgtctt ttgaagctgt
    17881 gctcaagctc tttctctacg cagttaatga agaaaaagaa agtttgcgtg atttgagtac
    17941 agctcttatc gaccgatcgc tccaactaga aaccgatgtg tcatcgatta gtcataccca
    18001 attgtctcga gcatttaatg cacttgaacc taaagtgtta gaagaaattt ttcaacaact
    18061 gttagaaaaa gttcaacatc aaatcaaacc aaccaagcga aatcatctgt atctgattga
    18121 ttccagtacg ttttcgctta gtttaaaacg tcataagtgg gcgactttcc gtcaaacaaa
    18181 atcaggtgtc aagttacact taaactattg ttatatggac gatactacga tgtatccaac
    18241 tgattttacc ttaacaaatg cccatgaaca tgatctcaat caactaccag tattggtgaa
    18301 tcaaccagag gcaacttacg tgtttgatcg tgggtattag gactttgaaa agatggatca
    18361 gatgcattgg gatggctatt tcttcgtcac tcggattaaa aaaaacacca aagttcacgt
    18421 gatagatact ctggaaacat cgccagaaac agaaattcta cgagacgagc tggttcgttt
    18481 aggatctaaa acctatctaa ccgccaattt ccgactagtc actgttcaag ataagaacgg
    18541 acgtgtgttt caatttatca ccaatcgaat ggacgtctct tcaaaagaaa tctctgatat
    18601 gtatcacgct cgttggcaaa tcgaattgtt tttcaagcac atcaaacaac acatgaccat
    18661 taaaaccttc ttttctcaaa gtgaaaaagg cgtgcagaat caattgattt taacgatgat
    18721 ttccgctctc ctcacgtttc tcatcaaatt ggagacgaaa acggaaaaat cagtgttcca
    18781 aatcaaacga ttctttcgtt atttactgtt tcagcccttt gaatgttgtc tagaaaaatt
    18841 aattcccaca tgagaaaaaa gaaatttggc tcgttacttt tggttttcag ttgaataaca
    18901 gataaaaaat tttctggaaa aagtcatcct ctgcgtcagc acagtttttt ttgacttatt
    18961 ttcagaggaa atagttttca gaatattcta aaatgtttta tgcaacacta gtgatatttt
    19021 ttagaattag atttctaatg tg
//
LOCUS       DACCXM010000050        18424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCXM010000050 DACCXM010000000
VERSION     DACCXM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.052"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(168..2252)
                     /gene="cadA"
                     /locus_tag="IVY61_001488"
     CDS             complement(168..2252)
                     /gene="cadA"
                     /locus_tag="IVY61_001488"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7970605.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(2777..3136)
                     /locus_tag="IVY61_001489"
     CDS             complement(2777..3136)
                     /locus_tag="IVY61_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP7970606.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3166..3507)
                     /locus_tag="IVY61_001490"
     CDS             complement(3166..3507)
                     /locus_tag="IVY61_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970607.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3504..4178)
                     /locus_tag="IVY61_001491"
     CDS             complement(3504..4178)
                     /locus_tag="IVY61_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7970608.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5354..6652)
                     /gene="asnS"
                     /locus_tag="IVY61_001492"
     CDS             complement(5354..6652)
                     /gene="asnS"
                     /locus_tag="IVY61_001492"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP7970609.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6692..7882)
                     /locus_tag="IVY61_001493"
     CDS             complement(6692..7882)
                     /locus_tag="IVY61_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7970610.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7903..8430)
                     /locus_tag="IVY61_001494"
     CDS             complement(7903..8430)
                     /locus_tag="IVY61_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7970611.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8477..11266)
                     /locus_tag="IVY61_001495"
     CDS             complement(8477..11266)
                     /locus_tag="IVY61_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7970612.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11413..11613)
                     /locus_tag="IVY61_001496"
     CDS             complement(11413..11613)
                     /locus_tag="IVY61_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7970613.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(12066..12386)
                     /locus_tag="IVY61_001497"
     CDS             complement(12066..12386)
                     /locus_tag="IVY61_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970614.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(12889..14007)
                     /locus_tag="IVY61_001498"
     CDS             complement(12889..14007)
                     /locus_tag="IVY61_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970615.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            14478..15785
                     /locus_tag="IVY61_001499"
     CDS             14478..15785
                     /locus_tag="IVY61_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7970616.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            15906..17105
                     /locus_tag="IVY61_001500"
     CDS             15906..17105
                     /locus_tag="IVY61_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7970617.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(17132..18400)
                     /locus_tag="IVY61_001501"
     CDS             complement(17132..18400)
                     /locus_tag="IVY61_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP7970618.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         5285 a         3942 c         3224 g         5973 t
ORIGIN      
        1 tattttttcc atcaaaaaaa taagttattt tcttttattt cattgacctt attgaatttc
       61 aagctatttt gaattttctg caaaattaaa aacattagtt attttataat cctatctatt
      121 ttctttacat ttttcattaa gaaaaggata taaaaaaaag aggacgttca ttcgtcctct
      181 ttttttacat gcaagcttcg catggcattc aaaacagcta atactgtcac cccaacatct
      241 gcaaatactg catctcccat tgatgcaaaa ccaaaagcac ctaaggcaag taccaaaatt
      301 tttatcccaa tagcaaagac aatgttttgt ttgacgattt tcaatgtttt ctttgctaaa
      361 tggattgctt ctgcaatctt cgatggttga tcattcatga tgactacatc tgctgcttca
      421 attgcagcat cgcttcccaa tccacccatt gcgattccta catctgctct tgctagcacc
      481 ggtgcatcgt tgattccatc tccgacaaat gcggtctttt tattgtttct ttgtaagatt
      541 tcctctaacc gctgcacttt atcttcagga agcaattcgc tatatatctt gtccacgcct
      601 acttgcttcc cgatatgatc agcaattttt ttcgagtcgc ccgtcaacat gactgtgttc
      661 ttcacaccag cctgttttaa ttgaaccaat gcgtctacgg catcttcttt caaggtatct
      721 gcaataacaa gataaccact gtaactttga tcgatcgcta gataaagcaa tgtaccgatt
      781 tcttgactta aaggaaaaga gatttcaaat ctttccatca atttgtgatt acctgctaat
      841 acttgtttgc catcaacttc agctaagatc ccttcacctg caaattcctg gatattagtt
      901 gttggtaaaa gcggacctgc gtagccttct aaaacagatt gagcaatcgg atgtgtagaa
      961 aactgttctg cactcgcaat atattgcatg aatttctttg gatcgatttt tgtatcgatc
     1021 gtctggatcg aaaagtcacc tttcgttaat gtgccggttt tatcgaatac cactgtctcg
     1081 gtttgcgcaa gcaactccaa gtaatttgaa ccttttacta atacaccgat tttacttgcg
     1141 cctccgatac cgccaaagaa actaagtggc acggaaatca ctaatgcaca tggacatgag
     1201 atcactaaaa atgtcaatgc tcgatagatc cattcagaaa acgccgctcc agaaacaaca
     1261 agtggaggta aaacagctaa taaaacggcc aatcctacaa cgactggtgt gtaatagcgc
     1321 gcgaatttag taatgaaatt ttcagctggt gcttttttac tgctggcatt ttctactaat
     1381 tctaataatt tgctgatagt agaattttca taagattttt ctacttgaac taaaaatggt
     1441 ttatttttat tgataaatcc gcttaatact gtttcaccaa catatacaga tttagggact
     1501 gattctcctg taagtgctga cgtatcgact aatgattctc cttcgatgat cgttccatct
     1561 aacggtacac gttcaccagg cttcacaagt acaaactgac cgatctgcac gtcttcaggg
     1621 ttcacggtca tcaatccttc cgtcgtctgc acgtgagctg tttctggacg gatcgccatc
     1681 aattcgcgaa tcgattttct tgaatgactg actgccaatc cttggaaata ctcgccgatt
     1741 tgataaaata gcatcactgc tacggcttct ggatattcac cgatcaacat agcgccaatc
     1801 gtagcaacag acataagaaa attctcatca aagatttttc ctttgacgat attcatcact
     1861 gccttcttca ctacatcata tcctatcagc agataaatca ataaataccc tatcaagcga
     1921 attggttcat taaaatcaaa taatagcaat aaagcgatac ctgcaaggga aaataaaatc
     1981 cggaacagcg tatttctctc tttttcattt ccatgttcag atactttttc cttatctatt
     2041 tctgttactt tgacatctgg ttctaattcc ttcactactt tttttgtttc ttgttcaatc
     2101 gtagccaaat gatcttcctc tgcgtcaatc gttagttttc cagaagtgaa gtttaccgtt
     2161 gcttccttga ctccattgat cttctgaact cctttttcga ttttcattgc acagttcgca
     2221 caatctaaac cttctaatct gtaacttttc attttaatga cctcctcgcc tcaacatatg
     2281 aacatctctt cataactaat tataaatgaa tggttattca tatgtcaagg tatccgaaag
     2341 aaaaaatgaa aaacatctgc atcggaaaat ttcttccata cagatgtttt ggcttattct
     2401 ttctttttct cttcgatcat tgcccctgct cttatccaac ccgcacttgc cttcttgatc
     2461 ttgacaggat agcaactaag tttgaagcct gttgctggca atttgtctaa attcattaat
     2521 ttttcgatag gacaatatct tttttcaatc cctgcaaaat gtccttccca gatattcgaa
     2581 gctccactac gctttcaaaa atatagaaag agttctttaa tgaaaattat ttcaactttc
     2641 cataaacagt aatcccatat aaataaaaga cgtaaaagtc acatgggaga acttatacag
     2701 gaaaacacat agagtcaggt gaaatacatg tatcccacct gactctatgc gttttctaac
     2761 ctttattatt ctctttttat acaatttctg caccgaatgg catcaacgcc aatcgagcaa
     2821 gtttgaaaga ttgcttggca aaaggtatcc ctatgatcgt gatacataga aggatgccgc
     2881 tgacgacatg tgcgagagca attggtaggc ctgaaacgat cagccagatg atattaacga
     2941 tcaatgacat gccagactgg tgataaatta cttctttgcc aaaaggcgca aaagaaaggg
     3001 aggcgatctt aaaacattgc ataccgattg ggatacctat gatcgtgata caccaaagta
     3061 tccccgctgc taaccagcta agaccaccga ttagtccgcc gaatataaac cagatgacat
     3121 ttcctaaaca actcattgta tttctccttc tattactgtt tttgattaaa actgggcaaa
     3181 aattgatttt attcttcttt ttttagcttc cagcatcaag tattctgcaa attttttttg
     3241 gatttcttcc gtattttctt ttgttactaa tgtgtgttgg attagttttc ctgatggtcg
     3301 ctgatccacc cgttcttctg actgttgcac gtccgcttgc gcaaatccga taatccgttt
     3361 ttcttttgtt acagcaacga gcaatagttt attcttatca tttaggtcag ctgccgtttt
     3421 tgcttttacg aatgattctg gatgatcagt aatcagctga ttgatttgag gtaaatcttc
     3481 ttttactgcc caccgtattt ccatcatttc tcacttttct ttcgctcatt tttatctcca
     3541 ctaaaaaaat cgactgcggc aattgcatct atccagcttt gtgcaatgat ctcatgtccg
     3601 ccaatcgttg ggtggacccc atcttctctt gtatatttcg aaaaaccata agcaatccct
     3661 aaagcattga atatgccatc taaaggaacc agcaatgcgc catattcctt agcaagttta
     3721 cgaacaattt ggattttagg gtctaagtct ttacgccagc catgacggtc gtcggttata
     3781 ggaagtacaa acggctccat caagatgatg ttgcagattt ccgctgtttg aagttcattt
     3841 agtaattttc gataaacgga ttcaaattgc tgcgcagatt tctcagttcc gaatatagcc
     3901 tgatcgctag tattatgcca ggtatcattg attccgatca aaatcgatac atagtcaggt
     3961 tgtaaagcta aacaatcttc ttgccagcgt acttgcagat ctgcaatttt gtctcctcct
     4021 atcccacggt tcatgcattc aaatttcatc gttggatatt gggagattag ttttccggca
     4081 atcaaaagtg gataaccttt acctagatca gttgcttctg ctcggtctct tcctgcgtct
     4141 gtaatgctgt caccgataaa taatatacgt tttttcattt gtcaaaaact cctttttgaa
     4201 taaaacgatg attttcttcc ttatattatc ccataagccc tatcaagacg aaagagaaaa
     4261 tatatgtata aaaaatgaca ccagcctagt cccttcaatc aattgaatga acgctcatca
     4321 agtgtctagt tttttatact ttttttctta acgtgccgga ttccccttga ttccttcgtt
     4381 gtttggtcca gagaaatcga tcacacctaa gcttccgtca atcagaccac tgatgacgcc
     4441 atcagaacat atatttatag gctcatctaa gacttccagt aaatgttctt ttactaatat
     4501 ctcttctaga ttgcttggga tagattggtc ataactttat ctggtccgat gatttcaagg
     4561 atgaattaaa cttacatatg gatcattgca tgctgaaaat acggacctac tccatcttcg
     4621 aatgttacta ttagtttgtt ttcaggtact agatgctgct ttatttcaac agcagaagca
     4681 tcatcaaatc agatttccat ttgaataacc ttctttgtat atgtttgatt gattgcgatt
     4741 tcgttataga accttgtgaa tcaaatagca atttcttttg ctttcttctc ttgtctgctg
     4801 gaaacttcct ttatcccgta ttttaaaaaa agaactgcga gagttcgcag ttcttttttt
     4861 taaataaacg ttgctctccg gccaactcct cggtcacaag tcacaatagt catcaaacat
     4921 gaaaaactgt tttttgaaat tactccttgt gactcggagc tgaacggcag gcgcacaacc
     4981 tcttgatata aacggtgttc aatcagctga ccctcgatat tagctaaaaa aacggtaaaa
     5041 tatgagaagc tatttttccg ttttgtttct ctaatactca ggtcaaggca ctgatttcac
     5101 aacctcttcc ttaaacggtg ttcaaaagcc agtttctcgg tattaagcca aatttatgca
     5161 aaaatgtgga aagccatttc cacaaaattt ttcttaatac tcgaaactaa acagcttttt
     5221 cacaacctct ccactaaacg gtattcaaaa gcttacatct gcggtaataa gtcccgccaa
     5281 ttcaaaaaca tgaacagctg tttttgcttg gctgttctaa ttacttgatg cagaacagct
     5341 ttttcatcac ctcttatgga taaattcggt tcaacaaacg tgggaatgga atagcttcac
     5401 gtacatggtc gataccagca atccaagtga ctgttcgttc aaggcctaga ccaaaaccag
     5461 agtgcggaac agaaccgtat ttacgtagat caagatacca tgaatattct ttttcatcca
     5521 aaccgtgatt acggatttgt tctagcaaga aatcatagtc tgttgcccgt tcgcttccgc
     5581 caataatttc accgtaacct tctggtgcga tcatatctgc acaaattacc acgtcttctc
     5641 ttgttggatg tggtttcatg tagaatggct tgatggcttt tggatagttt aggataaaga
     5701 caggctgttc gaatgaattc gcaatgaacg tttcatgagg tgaaccaaag tcttcgcccc
     5761 attcgatgtc atcaaatcca tttttcttca gtaattcgat tgcatcatcg taagtaatac
     5821 gagggaatgg caattttgta taagttttta agacttcttt gtctcgtcct agtacatcta
     5881 atgcatattc gcaatgatct aaaacatttt gtactaagaa tgctacatat tgttcctgta
     5941 cttctaaact ttcttcttga tgagtgaaag ccatttctgg ttcgatcatc cagaattcga
     6001 tcaaatgacg tcgtgttttt gatttttcag cacggaaagt cggaccgaat gaaaagactt
     6061 ttccgaaagc catagcagca gcttccatgt ataattggcc actttgagat aaataagctt
     6121 tttgatcaaa gtaatttgtt tcgaatagat ccgtagtgcc ttctggtgca gaaccagtca
     6181 agatcggcgg atcgattttg acaaaatgat gttgattaaa gaattcatac gttgcacgaa
     6241 tgatttcatt tctgatctgc ataatcgcat gttgtcttga agaacgcagc cataaatgac
     6301 gatgatccat caagaaatct gttccgtgtt cttttggtgt gatcggataa tcgtggctct
     6361 cgcttaccac ttccacattt gtaattccga tttcataacc aaattttgaa cgactgtctt
     6421 cacggatttc tccagttact aacacggatg tttcttggtg tagattttta gctgtttgga
     6481 aaacttcttc cgacacttcg ctttttacaa ctacaccttg gaaaaaggct gttccgtctc
     6541 ttaattgtaa aaatgcgatt tttccgcttg atcgcttatt tgcgacccaa gcgccgattg
     6601 ttactgtttc tcccacatgg tttttcgaat cgatgatttg aatttgttcc acgtatgact
     6661 ctccttttat attccttgaa tttcttcagg attaattttg acttttactc tcaataaatt
     6721 gcttgattct gcgtaccgct tcttctaacg tagcgcgatc agtggcataa ctcatgcgga
     6781 cattttctgg tgccccaaag ccttcgccag tcacaagcgc tacccctgtt tcttctaaaa
     6841 gatcttctac ccattgtgtg acattttcat acttacacat gcgtagcgtt tcttttacat
     6901 taggaaaaag atagaaagct ccttgcggct tttcaagaga aacacccggc agttctgcca
     6961 caagcggata taacgtattc aggcgttctt caaaggcgtt ccgcatttct tctactgtat
     7021 cttgctcacc tttcaatgct tcaacagctg catattgact gactgctgtc ggattgctag
     7081 ttgattggga agcaacagcg atcattccat tgatgatcac aggatttccc accgcataac
     7141 caatgcgcca gcctgtcatc gcatatgttt tagacacgcc gttgatgatg atggtctgat
     7201 ttttgattgc ttctgaaatc gttgcaattg gagtaaactc attgccatta taaactaaac
     7261 ggccataaat atcatcagca actatcaaaa tatcattttt tactgcccat tcaccgattg
     7321 cctgcagttc attcttgcta taaatcattc cagtcggatt cgatggagaa ttgatgatta
     7381 cggcttttgt tttttctgaa cgtgcttgtt ctagctgttc aacagtaact ttccagctat
     7441 tggattcttc ccctttcaca aagacaggaa gtccttctgc cagttttact tgctcaccgt
     7501 aactgaccca gtaaggcaca ggaatgatga cttcatcttg cgggtcaagg atcgtttgga
     7561 aaagggtata caaagcaaat ttcgctccgt ctgttacgac tgtttgtgaa ggttcataga
     7621 caagtccata gtttttcttc agataatcaa cgattgcctg tcgaagctct gggattcctg
     7681 ctgttggtgt ataatagcta gctttcccat tgcggattgc ttcgattgct gcttcttgga
     7741 tgttttctgg tgtcgcaaaa tccggttctc ccacagtcaa actcagcaca tctctgcctt
     7801 ttgcttttaa tgccttggcc tttgccgcag ctgccaaagt gacagacggt tctaatcgtt
     7861 ttgttctttt tgataactcc atcccattca ttcctttacc ttttaaattc ccttgattgt
     7921 cttgacttca tctccagatt caaagccaat caggtaataa ttcagtttcc catttgtttc
     7981 ttttcctact acttcccaca caggctgatc atcatacatc cctaaagcag ctttttcgat
     8041 ttggatttca ggatgggccg cagcaattgt ctgttttgct tcgttttcag tcagtccctt
     8101 tgtttgatcc aatactttta ctttttcacc agatttagga atgataacgg cgatttcttt
     8161 tcctttatca tttgtacctg tcagtgaaaa gtatgtcttc tttcgagtaa accaataaaa
     8221 atgatcggtt tcctcaagat ttgcatattt ctttgcgatt tctactgctt ctttcttcgc
     8281 ctgcaccgcc ggacgactgg aacgcaagta aaaaatcgag ctgatcacga tgaccgctaa
     8341 taaaaatacg atcaagccaa gtaacacgcg attaatcgtt ttttcctttg tctgtttttt
     8401 cttcaagggt cgtcgctcct ttatcttcat tccttttcat tatagcagat tttaattatg
     8461 aaaaaagaca gctcctctaa cttccttctt catttttctt taagaattca tggatatcct
     8521 gatagatttc ctccatagct agctctttga tcggtacttt tttcggtaat gcatggatga
     8581 tccgtgcgcc atattttgca gtgactagtc ttcgatccaa aataagcaat acgccacgat
     8641 ctgtctccgt ccttatcaag cgacctaatg cttggcgtaa cttcaaagct gctttaggta
     8701 cggattcctg gtaaaaagga ttgatcccct ctgattctaa ataagcgttc ctagctttga
     8761 ccatcgggcg aagcggatta tcgaagggta aacgtgtgac tacaacgatc tgcaaagcat
     8821 ctccaggcaa atctacacct tcccaaaagc tgtcagcgcc aaacaatacg cttttttctg
     8881 acaaaatgaa gcgtttcaaa agcttctccc ggcttccacc aaacccttga gctagtattt
     8941 ctcgtccttt ttctagcata gacagatgca ttttctgata gaccttctgc aaaacatcat
     9001 gagaagtgaa taacacaagt attggccgat cttcttgttc gatcagacct gtaagtgctg
     9061 tctctaaata atcgatatat gcttgattgc tgacttgatg gacggcaggt gcctcttttg
     9121 gcacatagat ccttgcctgt ttctcataat cataaggaga ggcaatcact ttaagaggtg
     9181 ctgcttcaat gccaagcctc ttagagagat agtgtcgatt agggccgatc ttcagcgttc
     9241 ctccaatata aagaatacga tcatatcgag gataccaaac agtcgacggc aacatagccg
     9301 cttgaaaatc atggagatga aaaatcatct gctgcgctgt gttcccagga acgaaccaat
     9361 ggatcaaatg atctcgccag tcttccagcc attgatccat cagcaagtat tgcttgtcta
     9421 attgatcaaa taggctatat aaacttgcta aatcgatccg catctggttt gtccagcttt
     9481 cttggataat ctcaaaagtc agacgcattt gtgactgtag ctgctggatc tcctgataaa
     9541 aaagctgcag gtgatcaatg gctgtcccag attccagtgg cagctgatcc aacatctctt
     9601 ttgtcatcat gacttcttct ggatactgtc cgttaggttg cttgccgctc actaaactca
     9661 ttaatccttc tgccatacaa atctgtgctt cgcatagcgc agaaagttct tctcgataaa
     9721 tcgataacca gcgtaaggct tcatgttctt tcggtaaata gctttgtatt cggtcaaaca
     9781 gctgatttgg ttcttctaat tgctggattt gtttatggaa atagttaata cttaatcgtt
     9841 tgtcatttac tttttcagcg atttccggca aatgatgtac ttcatcgatc aacaaaaatg
     9901 gactagctgg aagcaatggt tcttttcgct gtgtttcttg tactaaaaac gcatgattga
     9961 caatcaaaaa attactttgc ctgattttct tttgcaagaa acgaacaaag tcttgttcgt
    10021 aaaatgactg atttcttgct aaaaatccaa tacctctgtg cgcgatatct gcaaagaatg
    10081 aatggttcaa atttgttaaa tgtaattcat ccaaatcacc agtcttcgtt tgcgtcagcc
    10141 aaactaagac cgccatttga tacaaagcat attgtttttg ttctggcggt tccacaagtg
    10201 tgcctttaaa acgctgcaaa tcaatatagt ggcgatagct tttgataatt acaggttgta
    10261 tgggctgatc tagcaattga ttcagcaatg gaatgtcttt ttctatgatc tgctgctgca
    10321 aaactagcga aacggtactg atgacagcgg gtttttcagg tgtagccaaa taactcaatg
    10381 gcagcagata accgattgtt ttacccattc ctgtagctgc ttcgatcatc aaatcttttg
    10441 attcatcttt tgtgaaatgg tcgtagaccg cattcatcag tctgttctgt tcttttcgat
    10501 acatcagtgt tttaccaaac attttttctt ttgcttgttt ttttcttgga tatgtccgct
    10561 cgccataatg gacggatgtg aatagttcca cttctttttt ctgcaaagct agcccatcga
    10621 cgatttctaa tgagggatca agtggctctg gtttttcttt catttcttca atcacatgat
    10681 aaatgaaacg actcgtatcc atacctgtgt gtctacttaa tcgcgcaatc tgctctaacg
    10741 tgatgattgg caattttctc atccgttctt cgatcagcaa aagaagttga cctgtcactt
    10801 cggcatcact atctgcctga tgaggattct catgaataaa tccaaggctt tctgacaaat
    10861 cagctaagcg aaagcttggt tccgtcggca agaaaatttg tgcaagttct actgtatcga
    10921 tgcctggtat cttcaaacta ggaagtccgc atcgcatgag ttcatggttc aaaaaattat
    10981 aatcaaaata aatattatgc gcgacaaata tggtgtctgc caaaagattg taaatcgttt
    11041 gggcgacatc ttcgaaataa ggcgcttttt ggacgcgttg attcgtgatc cctgttaaat
    11101 gctggatttg ttttgaaatc ttccgccctg gatgaatatc gatcgaaaaa cgtgaagtga
    11161 tccgacctga ttcaatcatg acgcagccaa attggatgat ccgatcttct tttggattcg
    11221 tcccagtcgt ttcgatatca acgactgcat aagttcttgg tttcaaaata tcttcctctt
    11281 ctcctgcctc taatgttctt ttgaaattat actacaaagt gcagaaaaat actctcatcg
    11341 ctttttaaag aaaatagaca aaaacgccca gacacgcaaa tgtctgggcg tttatttttt
    11401 agtataggtt aattaagctt tagtaacgtt tgttgcttga ggtccgcgtt ggccttcttc
    11461 aacatcgaaa gttactgctt ggccttcttc taaagttttg aatccgtcgc cttggatagc
    11521 tgagaaatga acgaatacat cgtttccgtt ttctgcagtg atgaatccaa atcctttttc
    11581 tgagttaaac cattttactg tacctgtttc cataataaaa atcctccttg tgtatcaaca
    11641 catattttgc aattacttga atggtgtata tctggaagat gtttatttga aacaagctac
    11701 cacagattac cgaacaaaaa ctactcctat agtatatcat accgtttaca aaaaagctag
    11761 agtttttcga ctttttttat taaaaaaagt tttctcagcg attacataaa gtgaaaaacc
    11821 gaactttttt ttaaaataaa cgacatattg ttcagttggt gtttttttgc tctgatgttc
    11881 ttttttaaac taaaaaaatt attctttctg actacttatc ggttctgttt caacggtcga
    11941 ggctggggaa gattctttag taggtctgat gttctcatgg tatttctgat agcctatgac
    12001 agttaccaga agaacaaggc aaaccaatac gaccaacgag attagttttt cttttttgct
    12061 catatctata cctcataaat aacaggatga tgtccatcat atttctgagt atgagtaaaa
    12121 gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac tttcataatc acaggtcgga
    12181 cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt cttctgtttc aggaatcgca
    12241 gaatcaagat ctggtgtctg tacataaaca tttttgtaca gacgtgtgcc tgtaatagga
    12301 aaccgctgtg tttcttcagc aaaaactggc tgacttgtta aacttaggga tagagacagc
    12361 aaacaccata acagtctttt tttcataaag acctccttat cgataagttt ttcccaatta
    12421 aaatcatatc tcaagaacgc ccgttattca cattttgttc ataaatgatg ttttttgtat
    12481 cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc ttttttaaac ggtgttccag
    12541 aagtagcttc ttcggaaata agccgaaatt tcacaaaaat ttgagaaata atttttgtca
    12601 atctcttctt atttcttgaa gctgaccact tctgtcacaa cctcttcctt aaacggtgtt
    12661 caaaagcttg catctgcggt aataagccca ccaaacaaaa aatatgaaga gctatttttg
    12721 cttgctgttc ttattacttg atgcagaaca gctttttcac aacctctcat acacggtgtt
    12781 taatcagctg accttcggta ttagctcaaa aaacggcaaa atatgtgaaa ctatttttcc
    12841 gtttgtttct ctaatactca ggtcaaaaca ctgatttcac aacctctttt aataacgaag
    12901 aatcgctgtc agactctttt catctggcgc atctttttga tccaaaataa acacttttcc
    12961 gccattaatg atcacatcat ctgctaatgc attcaacact tgacgccgat catattctgt
    13021 ttcaggatct tcaccgaaac catcagctaa gtttgacgta gcgataaata aatgtgacac
    13081 ttttccttct ttcgcagctg gaacgatatc aactaactga tcgatgaatt tcttatctaa
    13141 gagtttattg tattcttttg attcttgagc tgcgagttca cttgacagct tcttcgcccc
    13201 ttcttcgata tctctaaagg atagctgcgc aggagaagca gaaatcgcaa cgtctgcaca
    13261 ataataggga tttttagcaa tttttttgaa catcgtctga ttttctggta gagcaaataa
    13321 ataaatcggc cattgttctg gattttcgaa agaatccttc aagtacgtat caactgcttg
    13381 gtaatagttg acccaatcga tctctacttc ttcatctttt ggatttacgc catggaaagt
    13441 tactccatct cgggctccgc ctcctccatt agaagagaag ttcaaaccgc cacctgtcag
    13501 ttcagatccc aatgcagtaa cgacatcaac cggtgcccct tctggtagag atacttcttt
    13561 tacttgtttg ttgctgactt catacaattt catggagtcg cggttcaatc ccaataaata
    13621 ataagaatag ttgaactgcc catttttgat aatagccagt aaataaggaa gatcacttac
    13681 atagtattga tcgtctaccg ggatactcaa acgatggata taggtttggt cagcagttat
    13741 aatgaccgct acactcgtag ttgcactacg ccaaaacgaa ggatctccta acaaattttc
    13801 aattttttct tggaacgggg accagtcatg ttcggaatat tttttctcaa aacgtttctt
    13861 tgcttctttc gcaaaatttt tgaatttgat ctgatctttt tccacatctt gatgggcgac
    13921 atgcgtattc agcatgatcg tgacgaatgg tcctttagcg ccttctgaag tcaattgtga
    13981 caataattct ttatcctttt ttgccataaa cgtacccctc ctaaaaaaat cattcctttg
    14041 tttctatcta aagtctacca caacactatt tttccataca ataaaacgta tctaaaaaaa
    14101 taaaacggtt tctacggttt taaatttagc tattttttta agttatcttc ataatttcct
    14161 caattattta gccccagtct tcaactattc ttcatgaaat gctgataaac taagaaaaag
    14221 aataaaagag gtggatctta tgcatttgat tatgattttc agtttattaa gtattatcag
    14281 tttatttgct ttcagcgctt cccgcttatt agatgcggaa acacagccta ttcctgtaaa
    14341 agtacgtaaa aacgaccgct aaccttccat aacgtgcgtt cttatcctaa agttttttca
    14401 gaaacattga aactctctcc cctctctgat aaaattttaa tttgtagcga aacaaattaa
    14461 ttacaaggag atcggatatg tccataaaaa aaacacgtct agtcctatta ttggctagtc
    14521 tcattctttg ccttgcttct tttctaccag ctgttgctgt gcaagctgaa gatactttca
    14581 aagtaaatgc aaaagcagcc tttgctgtag atgcgcaaag cggtaagatt ctttatgatc
    14641 aagatggaga aaaaaccatg gggatcgctt caatcacaaa aatcatcggt ctttacatcg
    14701 tccttgatca agtaaaagaa gggaaactat catgggatga taaagtttca atttcagatt
    14761 atgcagaaaa tttgagtgtc accccagatt tatcaaatgt tcctttgcat aaagaaaaca
    14821 cctacaccgt taaagaatta ttcgactctg caatcatcca gtcagccaac gcttcaatgg
    14881 ttgccttggc agaaaaaatc tctggcagcg aagcaaaatt cactgaacga atgaaagaac
    14941 aattgaaaga ttggggtatc aaagatgcaa cgatcgttaa tgcatcagga ctgaataact
    15001 cttatcttgg cgaaaaccga ccagaaggta ctggagaaaa cgacgaaaat caaatgtctg
    15061 ctcaagatgt ggctatcgtt gcccgtcatt tgatcttgga ttttccagaa atactggatg
    15121 tatctagcac tacgacacaa atgtttggcg aaaacacgca atcaccagta gaaatggtca
    15181 actggaactg gatgctccca ggattcgtga attataaaga aggcgtggac ggattaaaaa
    15241 ctggtacgac tgaacttgca ggagcctgtt tcgtcggtac gatcactaaa aacgatcaac
    15301 gaatcatcac tgtcgtattg aatgcagacg gccatgcgga aaacccaagt gcccgtttca
    15361 atgaaacagc tcgcttaatg gattattgct acgacaattg gtcagaaaaa gagcttggaa
    15421 aagccaatgc aagtatccct agtctaaaaa ccattgatgt aaaagacgga aaagaatcaa
    15481 cagtgcccgt tgtgcttaaa agtcctgtaa aagtatgggt acggaacgat atggatactg
    15541 gaaaattaac aatcacacca actcttgaca aaaaacaagt cactgacaat gagatccaag
    15601 cacctatgga taaaggaaca aaaatcggta ccgcaacgat caccttggca gaagataaat
    15661 taggttatct tgaagacaac aacgcgccat ccacagaaat catcacggat aaaaccgtag
    15721 aaaaagcaaa tattttcgtt attggctggc gacgcgtcgc agaatttttc ggtaacttgt
    15781 tttaattgaa caaaaagaaa aaagcatctt tccatgtgga agatgttttt ttgctttttc
    15841 tagtttaaat aacagctttt ttcatctatt ttcgttatac tgtataagaa aaaggagttg
    15901 aatttatgaa tctaaccaga gatctgattt ttttggttct ctatcttatt ggggtattct
    15961 ccatcgttta ttggcgaaaa cagaaaaagg atccttcctt gttcttttac agcagtacat
    16021 ggacgtttgc ggtaatcttg acttattttg tcgtgttgga aattttttat tcgacctttt
    16081 attttttgat tcccttagtg tttttcagta tctttgcctt ttcctatttt tcagaaaagc
    16141 gaaggctgtt gaatggtctt ttattcaata ttttcctcat tagttttggt atctacttgt
    16201 ttgtcttgct atacgaaaca caaaatattt ttcttggcgg actgattgcc ttgatcacga
    16261 tcccgttact gcttgtcttt ctattcggca tttatggatt gatcgttttt cttttttgga
    16321 acggtgtcac agttttacgt agagagtcgc attcattagc taatctttta acgttgatac
    16381 tagccatttt tcttacattg tttcttgttt ttgacttttt cttgttaaaa tatttaccac
    16441 aatggataaa tgctttgttt ttctgcatcc cattgatctt gatctatcta tttatcgtat
    16501 tctacaactt tttgactgtc tcattcttgt atcagttcaa ccgccctcga tacaaccaag
    16561 acttcatcgt tgtgttaggc gccgggttga tcaatggaga aacagtttct cctcttcttg
    16621 ccaagcgaat caataaggcg attgcttttt attgggcaca aagccgtgcg acattaaatc
    16681 cgcctatcct attaatgtcg ggtggacagg gagcagacga aaaagtacca gaagcaatgg
    16741 cgatgaaaca atatgccatg gaacaaggaa ttcctgaaag agatattctt gtcgaaacca
    16801 attcgacaac cactttggag aatatgcgct attcaaagga aatcatggat cagcaaatga
    16861 aaggtcccta tcgcgcgatc ttcagttcca acaattacca cattttcaga gctggtctct
    16921 atgcacgaca agcgaaactg aaggcaaatg gcattggggc aaaaacagct ttctattact
    16981 tgcctaacgc gtttttaaga gaatacatcg ccatcttgat gatgaacaaa aaaagacata
    17041 tgatcatttg cggattgctt ttcgcaggtt ccgcattctt atcactgatc actttacttt
    17101 tctaaaaaaa caaggtgtga caaaacatat gtcacacctt gtttttttct ttcattctct
    17161 taactattgg caaaaagagt atcccttttc tgaccgctac ttgctgagca gaataaatac
    17221 cgctgttgcc tgaacacata aagctgatca agcagatcat aaagaagtat actgccgctt
    17281 gtgaaccgaa caattcgatc cccatcacaa agcaggcaat cggtgtattt gtcgcaccag
    17341 aaaatactcc gataaagccc aatcctgcaa gaaaaggaac agaaacatgg agcaaaggcg
    17401 ctagtgctgc accaagcgtt gcaccaattt caaacaatgg tgtcacttct cccccttgat
    17461 atcccgcgcc taaagacaaa actgtgaaga ataacttccc aataaaatca tagacatgtg
    17521 cctgtccatt aaatgattcc tgcagtaatg gaagactcaa tccgagataa cgttgtgttt
    17581 gcaaaatcaa tgctgcggct acaactacag ccccacctat cgtatttcgc caaacaggat
    17641 gaggaatcca ttgtgcatat cgcgctttta aaaagacgat cgaacggctg aatacccaac
    17701 caatcaaacc aaaagcaatc ccagcaacaa aaactttgaa gaacagcaac atactccatt
    17761 caggaatcac acccatttgg taatgtagat gagaaacacc atagctttct gtcataaaat
    17821 ttgcaaacag tgcagcaaag aagctaggga agatcgcttc tgttcggact ttcccgattg
    17881 ccaacacttc taatccaaat aatgttccag ctaaaggtgt gccaaataca gaactaaaac
    17941 ccgcactgat tccactgata atcaagattt cccgttctga tttatccaat cggaacaaac
    18001 gtgctgtatt atctgctaac gctcccccca tttggactgc tgttccttca cggccaacag
    18061 atcctccaaa taaatgggta gttatggttc caaacagtgt aagtggaata agtcgcaacg
    18121 ggattttttc ttctccacca tttccttgat cgatcactaa gttgtttcca cgactagaaa
    18181 gaccgccgtg atacgcatag agataagcaa aaagcgcacc actaactggt agtagataaa
    18241 ggagccaagg atgtgatagt cggatatctg tcacaactgt caggctattt aagaaaaaag
    18301 cagataagct ccccatgaat ataccgagaa tcgctgtgat caccagccac ttcagcatgt
    18361 acactaaaat gcgtactgga ctcggtaatt gtttttccat aaaaaattcc tcctcaataa
    18421 aaaa
//
LOCUS       DACCXM010000051        17987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCXM010000051 DACCXM010000000
VERSION     DACCXM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.053"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(68..1546)
                     /locus_tag="IVY61_001502"
     CDS             complement(68..1546)
                     /locus_tag="IVY61_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7970619.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1844..2779)
                     /gene="murB"
                     /locus_tag="IVY61_001503"
     CDS             complement(1844..2779)
                     /gene="murB"
                     /locus_tag="IVY61_001503"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP7970620.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2852..3772)
                     /locus_tag="IVY61_001504"
     CDS             complement(2852..3772)
                     /locus_tag="IVY61_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7970621.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3885..4637
                     /gene="xth"
                     /locus_tag="IVY61_001505"
     CDS             3885..4637
                     /gene="xth"
                     /locus_tag="IVY61_001505"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP7970622.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5501..6034
                     /locus_tag="IVY61_001506"
     CDS             5501..6034
                     /locus_tag="IVY61_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP7970623.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6524..7048)
                     /locus_tag="IVY61_001507"
     CDS             complement(6524..7048)
                     /locus_tag="IVY61_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7970624.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7041..7514)
                     /gene="tsaE"
                     /locus_tag="IVY61_001508"
     CDS             complement(7041..7514)
                     /gene="tsaE"
                     /locus_tag="IVY61_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP7970625.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7637..8617)
                     /gene="pta"
                     /locus_tag="IVY61_001509"
     CDS             complement(7637..8617)
                     /gene="pta"
                     /locus_tag="IVY61_001509"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP7970626.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8770..9450)
                     /locus_tag="IVY61_001510"
     CDS             complement(8770..9450)
                     /locus_tag="IVY61_001510"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7970627.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9576..10445
                     /locus_tag="IVY61_001511"
     CDS             9576..10445
                     /locus_tag="IVY61_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7970628.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10471..10986
                     /locus_tag="IVY61_001512"
     CDS             10471..10986
                     /locus_tag="IVY61_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970629.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11033..11596)
                     /locus_tag="IVY61_001513"
     CDS             complement(11033..11596)
                     /locus_tag="IVY61_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7970630.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11651..11830)
                     /locus_tag="IVY61_001514"
     CDS             complement(11651..11830)
                     /locus_tag="IVY61_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970631.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11907..12416)
                     /locus_tag="IVY61_001515"
     CDS             complement(11907..12416)
                     /locus_tag="IVY61_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970632.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12512..12892)
                     /locus_tag="IVY61_001516"
     CDS             complement(12512..12892)
                     /locus_tag="IVY61_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7970633.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13222..13971
                     /locus_tag="IVY61_001517"
     CDS             13222..13971
                     /locus_tag="IVY61_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP7970634.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14155..15432)
                     /locus_tag="IVY61_001518"
     CDS             complement(14155..15432)
                     /locus_tag="IVY61_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970635.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15511..16155)
                     /gene="pcp"
                     /locus_tag="IVY61_001519"
     CDS             complement(15511..16155)
                     /gene="pcp"
                     /locus_tag="IVY61_001519"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP7970636.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16354..17298)
                     /locus_tag="IVY61_001520"
     CDS             complement(16354..17298)
                     /locus_tag="IVY61_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP7970637.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17300..17986)
                     /locus_tag="IVY61_001521"
     CDS             complement(17300..17986)
                     /locus_tag="IVY61_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP7970638.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         5229 a         3679 c         3117 g         5962 t
ORIGIN      
        1 accttttctt tgaaaaaaac ataaatcagc taataaatat gtgagacaaa gctgccatcc
       61 ataaatacta gctgattttt tcagttaaat gtctattttt ttctctgatg atggtatttc
      121 caaaatattc ccagtcatct ggagtcggtg gatttttcca aattatctgt tctttattaa
      181 atttttcaag taaacaatca gataaataaa tattcgtacg agaagtgatt cgactctcaa
      241 tcacaatatt gtaattttta aatcctttca cttgagaaat aatgtaatcg gtataagcag
      301 atccttgcga aaaatcaata cagacatgaa tcggtgcttc aaaatatgat accggacaaa
      361 taccgaccaa taaaaagaga taatcataaa ataagcgaat catgaaacgt tcatcttttc
      421 ccaagaaata gcgtttattc tcttgtatga ccttttggat tgctgtactg aatattggat
      481 acgtctcgaa gaaaaactgt agctgctttt tagacgtaaa gctagtcatc tcaaagtcga
      541 atatccaatt ttttcgattg acatgcatca tacccatacg aagttctttt cgtatatata
      601 gtctttcttc ttcagagatt tcaatcgaaa atggtaattg atcaatcaat tccactgaca
      661 ttttttgact aatcatgtgg atatcatgaa aatgagcttc cgcaagtcca aatgcgccat
      721 ctaattcaaa ttcttcttcc gtgatcaaaa acaacatgat ataagaaatc tcatcaatga
      781 tctgctcttt agggatatga agaaaagaaa tatgtgataa atccttgatg atcttcattc
      841 cttctgagct cttaaagtat tcatctgaaa agaaacgttt tccgattggt tgattcccat
      901 aggccctcaa caaactaata gtggttagca aattgaattt gattcgtttt gttgcagaca
      961 gttttaaatt gaacagatat tcaaaatatt gcacgatact ctttgcttca ttcatgacgc
     1021 tttctctaaa tggcatagaa aaaccattaa agaccccgaa atatacccca aataatacgt
     1081 tccgtatgtt tatttcttcc ccaactaata tgtttttctt taactttatt tgatgctgtt
     1141 caagaaaaga gactaattct cttcttttta cataggctct tgatatgctt aaaaaatgat
     1201 tctctgcaaa tttttcgatc gttacttttg aatccacgat ctccgttaac agtcgagcaa
     1261 tttttgatcg ttgaaagtag attcttctta aatttttgat atccgtcaaa ttcagacgtt
     1321 cgacatggat ctctgcttct tctgaaattt ggatctctgg ctttgttttg aattcagtaa
     1381 attctttatt taattctatg atataatttc ttgttttaaa tttagacaaa cctaatgttt
     1441 catataactt aggcagcact acataattct ttttctcaaa ttctaaatat ctagctagca
     1501 gcagtttttc tctatcttcc tcttctataa ggtaatccaa cttcatatcc tacacctcaa
     1561 aaaatgcctc ataccaatta cttgatatgc agatgaaagt tcatcttttc acctatactg
     1621 attttatgat ggattatgct tgttttttca tcagttattc tcatttttta tctttttttt
     1681 gagaatattg caaaaaatgg aaaaatttat gaggaaatat tctatatttt ttgttttatt
     1741 gtgttaaaat gtacaagatt tgcgccattt gcgtaaacaa ccacaaaaaa gagcaggacg
     1801 gccaccgtcc tgctctttta cttattttaa gttccattac ttattaattg gctaaacttc
     1861 tgcttgtttc tgatattgct tgtttttctc caatgatccg cacttctgtt tctaaagaca
     1921 catcaaattt ttctttgatt acttcttgga tgtgcgcaat caattcaata taatctgtcg
     1981 ctgtcgcatg atcgatattg acgatgaatc cagcatgttt ttcagagact tgtgcaccgc
     2041 cccatttcag gccttgcaat cctgcttgtt ggatcagctg acctgtatag tgtcctactg
     2101 ggcgcttaaa gacacttccg catgaagggt attctaatgg ctgttttgat tgacgcaatt
     2161 cggtcaactc atccattctt gctttgattg cttcataatc ccctgtttgc aaactgaatc
     2221 gagcttccaa tacgatagca cgtaattctt ggactttact gtgacgataa gaaaatacca
     2281 tttcgtcttt tgtcaaagtt ttcaatgttc catcttcaag caaaagatcc acttcagcaa
     2341 aaacatcttt gatctctccg ccatacgcac ctgcattcat ataaaccgct ccaccgacac
     2401 ttcccgggat gccgcaagca aattcaaatc cagtcaatga ttctgccaaa gctgcataag
     2461 tcgtatcaat cagtgaggca cctgcttccg caatgaccat cgttcctttg acatgaatct
     2521 gtttcatttc agtcagcatg atgactacac cgcgaatccc gccatcttga acgatcagat
     2581 tgcttgcatt tcctaatacc atccaaggta tatcatggat tcggcaataa tcgatgattt
     2641 gctttacttc ttcttgtttt ttcgggaatg caaggacatc agctggtcca cctgttttcg
     2701 taaacgtata gttcatcaat ggttcatcaa acaatatagt aatcgtggct aagctttcaa
     2761 ctatttcttc tttattcact gaggattttc ctttcttttc tcaaacgtcc tcattattct
     2821 atcacgctta atacaatctt gccagtcttg ttcaaagttg atgacggtag tgatctttaa
     2881 gcacttcctc acataattca tgacttctta caacttcttt ttgtttaagc aattctgttt
     2941 ccgatttacc taaacggatc tgagaaaaga aggcctctac cattggctca aacccgcgtt
     3001 tgactagcgt gtttgtccag tcgccaaatt ctttcacttt ttttccatca gcatcttgaa
     3061 tgacaagctg ggtcagatcg tttacttcat aagtaccatt tttacttgtt acttgatatt
     3121 tttctatatt agcgccactt gctaaatcca tcgttaaaat agccgtttga ttttgtgttt
     3181 ctaattgtaa aatggcatat tccaaaaatt cctttgtttc tcttatctgg tattttgttc
     3241 tcaaaatggg ttcatctaat aaatacacag ctgtatcaat aagatgtaaa aacaaatcat
     3301 aaaggacgaa ctctgtcgtt tcttttgccg ctattcgatt tttttgcagg tggatcaacc
     3361 gcttatctgg gatacgtttc aattcttcta ccataggagc aaagcgccgg ttgaaaccga
     3421 tcatcaaaat tatttggttc ttatcagcta attcttgcaa ttcacgaact tcatttagat
     3481 ggacactcag tggtttgtca atataaacat ggataccatt ttctagacat tttttggcta
     3541 attcataatg tacttttgtt gctgcgtgga tcatacatgc ttcgattttt tcttctaaaa
     3601 gctcatcgat cgtttcatat agatgttgga acccgtactc tttttgaatt ctttcttttg
     3661 tttctttatt tcttgttgca aaataaaaat caagtgttcc ttgttttttc gcataagttg
     3721 gtaaatatgc tttttctgcg atattcccta caccgattac accaatcttc atctactttc
     3781 ctcctggatc atttagttac tttcatctta caccattttt ttctaaaatg aggtacaata
     3841 gtatcgcaaa ggtaaggaaa ttaattcgaa ggagtgtgct tttattgaaa tttatctctt
     3901 ggaacgtaaa cgggcttcgt gccatcgtaa ataaaaattt tttagaagtt tttcatgagt
     3961 tagacgccga ttttttctgt ctgcaggaaa cgaaactcca agcaggacaa attgatttag
     4021 atcttccagg ttattatcaa tattggaatt acgcggaacg aaaaggctat tccggcacag
     4081 cgatttttgc taaaaaacca gcattgaatg ccacttacgg aatgggtatc gatatccatg
     4141 atacagaagg ccgtctgatt actttggaat attccgattt ctttcttgta acctgttaca
     4201 cgccaaattc tcaaagcgaa ttaaaacgtt tagattaccg attagaatgg gaagaagcct
     4261 tttataatta tttggaaaat ctaaaaaaac aaaaaccagt catcgtgtgt ggggatctaa
     4321 atgtcgctca tcaaaagatt gatttgaaaa actggaaaac gaatcaaaaa aatgctggat
     4381 tcactccaga agaaagagcg gctctttctc gactgttgga taacgggttt atcgatacat
     4441 tccgttattt ttatccaacc caggaaggtg tctactcttg gtggaactac cgattcaatt
     4501 caaggaaaaa caatgctggt tggcggatcg attacttttt gacgagcaaa gatttagagc
     4561 caggactagc tgatgcaaag atccatacgg acattatggg cagtgatcat tgtccagtag
     4621 aattagattt gaaataaaga ggttgtgaaa aagtcgttta gtttcgagta ttaagaaaaa
     4681 ttttgtgaaa atggctttcc acattttgca taaatttggc ataataccga gaaactggct
     4741 tttgaacact gtttatcgag agattgtgaa aaagctgtcc tgcatcaagt aataagaaca
     4801 gccaaacaaa aatagttcct catatttttc gtttggttgg gcttattacc gcagatacaa
     4861 gcttttgaac accgtttatt aagaggttgt gaaaaagctg cactgcatca agtaataaga
     4921 acagccaaac aaaaatagtt cctcatattt ttcgtttggt tgggcttatt accgcagatg
     4981 caagcttttg aacaccgttt attaagaggt tgtgaaaaag ctgcactgca tcaagtaata
     5041 agaacagcca aacaaaaata gttcctcata tttttcgttt ggttggactt attaccgcag
     5101 atgcaagctt ttgaacaccg tttattgaga agttgtgaaa tcagcgtcct gacctgagta
     5161 ttagagaaac aaatggcaaa atagttcctc atattttgct attttttgag ctaataccga
     5221 gggtcagctg attgaacacc gtttagtgga gaagttgtga aaaagtcgcc tactttcgag
     5281 tcgtaagaaa tttttttgaa aatggttcct cacaactttg cataaatttg acttaatacc
     5341 gagaagtcat gatccccatc taactccaaa tcacaaggaa tgagttcaaa aactagcttt
     5401 tctcaacaaa tattacataa aaaattctat gttttttgtt aaatttattt gcttttttca
     5461 aaagtatgtt acagtaattt cccaaggagg ttttacagaa atgaattttg ttgccatgga
     5521 ttttgaaact gccaatcatc agccttatag tgcttgttct cttgcgctag tcatggtaaa
     5581 aaatagccag atcgttgatg aattctatac attgatacag cctgagactc cttttttctg
     5641 gcgaaatgtc caaattcacg gaatccatca agaagacgta cgcaatgcgc cgaaattccc
     5701 agaagtctgg caaactatcc aaccatattt tcaaaaaaat cgtttagtcg ttgcacataa
     5761 tgctgctttt gatacaaagg tcttagcggg atgcttagat tattatcatc ttgaacagcc
     5821 taattatctt tctttatgta cagtgaaaag tagccgtcgc ctgtttcctg aatttccaaa
     5881 tcatcgattg aacactgtgt gcgaaaattt gaacatccaa ttagatcatc accatgatgc
     5941 attggaagat agtcgtgcat gtgcggaaat cctgctttat caagaaaaaa aattcgggac
     6001 agatccattg aaaaaacttg tgactataaa gtaaaagaat acttatgaaa cacacaaaga
     6061 ggtcgtgagc ctgccgtttc actccgagtc acaaggagca atttcaaaaa atagcttctc
     6121 atgtttatta aatattgtga ctggtgaccg aggagctggc aggagaacac cgtttattgg
     6181 agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagccaaaca aaaatagctt
     6241 ctcatatttt tcatttggtt ggacttatta ccgcagatgc aagcttttgg acaccgttta
     6301 ccaagaggtt gtgaaatcag cgttttgacc tgagtattag agaaacaaac ggcaaaatag
     6361 ttcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
     6421 attcaaaaaa aatggactgc gacacgactt ttgtcacagt cccttcgtgt gtttttctgc
     6481 cttctcaaaa ggatgacagt ttctcactct tttcctattt cttctattca atcaaataac
     6541 tcattttata tacatctaaa aattcaccta gatctgttct tgcccctcta ggcatgaccg
     6601 cttcgatttg gaagcccatt tttcggtaaa ggtgtacggc tctttcatta cgaacttgaa
     6661 cgtcaagctc caatcgaaaa agtacaccca tttccttcgc ccaactgatg atttcttcta
     6721 gcataagagt gcctagtccc attccccaat attcctgcag aatactgata ccgacttctc
     6781 ccacgtgaga caaacgaaat tgactgtctg ccttgacaga tgctgttcca ataatcgtcc
     6841 cttcatagat tgcaagcagc aataaattat tattgctttc gtatatataa tccaactctt
     6901 cttctaaggt ttctggtggt aacaggagtt ctgcttcatc caaaactaaa aattctgtct
     6961 ccttattaac ttgtcccatc atcaaaagaa tgtcttttgc atctcctggc actgcttccc
     7021 gcaagatgat cccaacttct tcactcatga ttttcagccc atttttctag aattcgctgt
     7081 ttgaaggttt cactttgctc cccgtatgag attagtttca cataccgctt ttctaaatct
     7141 gtatcacttt tttctaaaat cagttccaaa taatcctccg gcagtgcttc ttctaataga
     7201 tttccccatt caacaacgga aagcccatca ccttcaaaat attcatctaa ccctaaatct
     7261 gctccgctag ctgctacccg ataaatatcc atatgataca acggaatccg accttgacta
     7321 tattctctaa taatcgtgta tgtcggactt ttgatcattt gcgttatccc taatccttgg
     7381 gcaattcctt tcgtcaacgt tgttttaccc gcacctagat caccagtcaa tatcagattg
     7441 tcacctggca ttgcggaatg accaattgcc tctgcgaatt gttctgtcat tgttaatcct
     7501 gatagttcga tcatttttca tcttctcccg ctcttctttc tttttgccag tatatcgcgt
     7561 aatgaagttt ttttaaagaa aaaatgaact agtcaagaac agcagctgct gtttttggct
     7621 agttcgcgtg tatttgttaa tcaatcaatg tttgtgctgc tgtgataatc gataatttat
     7681 atacatcttc ttcatttgct ccacgagaca aatcagaaac cggtttgttc aagccttgta
     7741 gaattggtcc gattgcttca aagttaccga aacgctgagc aattttatag ccgatatttc
     7801 ctgactgcaa ttctggaaat acaaagactg ttgcttgacc agctacttct gaatctggcg
     7861 ctttcagttg tgcaactgaa ggaacataag aagcatcaaa ttgcaattcg ccgtcgatca
     7921 tgtattgtgg cgctagtttt ttagcgattt tagttgcttc aactactttg tctacttctg
     7981 gcgatttcgc tgagcctttt gtcgagaagc tcatcattgc tacttttggt tcgatatcga
     8041 acaattctgc tgtttttgca gaatctacgg cgatttcagc caattcttgc gcagttggat
     8101 tcacgttgat cgcacaatcc gaaaagacat atttttcttg atctcttcca cgtaccatga
     8161 tcatcgctcc gcttgtccga ctgatgccag gctttgtttt gatgatctga agtgctgggc
     8221 gaacagtgtc gccagtcgaa tggatcgctc cagagaccat tccgtctgca atccccatat
     8281 acgtaagcat cgtaccaaag taattcacat cttttaaaat cttttctgct tgttctttgg
     8341 atgcctttcc atttctacgt tcaacaaaag tatccaccat ttcatcccac ttatcgtaat
     8401 tatcagggct gatgatcgta aagctagatg ttttgattcc tctggcatgc gctgcatttc
     8461 ttatttcttc ttcgttccct actaaaatgg gttccacaag ttcttctgct ttcaaccggg
     8521 cagctgcgcc taaaatacga gcatcagtag cttccgggaa aacgattttg atcccacggc
     8581 gaacaatttt aaatcttaaa ctatcaaaca attccacaag atgacctcca gacgttttta
     8641 tatgtattat catacactat tccagaaaaa aagaacagtt tattttatct gtatcagtca
     8701 aaaatgctgg aaaaattaag tttttcacat acaaagcgaa ctgcactata acaggaaagc
     8761 gctgttttat tacactgttt caggcagttg ccaatcaatc ggttcttctc ctaacgcaag
     8821 gagcgcatca ttcgttttcg agaagggttt ggaaccaaaa aagccacgac ttgcagataa
     8881 cggactggga tgtggagatg ttatgatgat atggcgactc ttatcgatca ttttgatttt
     8941 ttcttgtgcc ggttttcccc acaagataaa cacgattggt ttttcccgtt catttaattt
     9001 ttcaatgatc gtgtctgtta atctttccca acctttcccg cgatgagaat acgcttgccc
     9061 ttcccgaacg gtaagaacgg tgttgagtaa aaggactccc tgtttagccc atgacactag
     9121 atttccatgt tgaacaggtg cgattccaag atcatcataa agctctttat agatatttct
     9181 taacgatggc gggatcttta cgccaggttg cacagaaaaa gataagccat gagcttgatt
     9241 ttctccatga taaggatctt gtcctaaaat caccacttta acttcttcat aaggagtcaa
     9301 ttccaatgct tcatagatat gatacatatc tggatggatc ttttgggacg catattcttt
     9361 ttttaaaaat tctcttagat gcagataata atcttttgaa aattctgagg acaaaacttc
     9421 ttgccaactg ttatgaatga tttctttcaa aaactccacc tcctaataaa aagaataact
     9481 cacattttct tcaatcacaa gggacaatct tcaccctcag cacttgatta atatggtaaa
     9541 ctaggaacaa gaagcagaaa ggaacgataa tcattatgat caaattaatt gcatcagata
     9601 tggacggcac attattagat gcccatatga gtgtttctcc tgaaaatgcc gaggcgatac
     9661 gttttgcaaa tgatgccggt gttgagttca tggtcgctac tggtcgaaac tatcaagaag
     9721 ctcgcgcagc tttagatgag gtaggaatcg attgtgcgat gatcactttg aacggggcac
     9781 aggtatttga taaagaagga aattcattgt ttaccgtgtc gattccaaat aatcaagcaa
     9841 tctctgtttt agatattcta gatgctaacg gtatttacta tgaagtagca accaacgatg
     9901 gtctttattc tgaaaatcaa gcgaaacgaa ttgaaagttt tgcttccatg gtggctaccc
     9961 atttacctca ccttacgtat aaaatggcga ttgccatggc ctcagctaag ctggagttat
    10021 tgcacatcac atacgtggac agtattcgtg ctattttgga agaaaagaat ctggaagttt
    10081 taaaaattat ttgttttcat acagaagggc cagctgtatt aggtccagtt ggaaagcaga
    10141 tttctctttt aggcgatcta gccgttactt cctctggtca aaacaatatc gaagtgaacc
    10201 ataaaaacgc tcaaaaagga attgctgttg cccatgtcgc tcacgagcgt gggatttctt
    10261 tagaagaagt catgacgatt ggcgataact tcaatgatgt cagcatgctg caagtagcag
    10321 gagtcagttt cgcaatggga aatgcagaaa tcgaagtaaa agattatgcc aaatatacaa
    10381 cggacacaaa ccttgaatct ggcgtaggaa aagccatatt gcgcgcgatt gcagaagact
    10441 tgtagtttta tctagaaaag agggatatag atgtcagaat ttttcattca ggaaaagcag
    10501 cttggacgtg caactcggac aattgtcaaa aacgaaaaag gacattccct gtttttgatg
    10561 gttggtcgct ggggaacaaa gggcgatgca ttgtctcttt atgccatgaa tggcgatctc
    10621 gttgctcaca tcaagcagat aagcctgaca ttcggtacgc gttttgagct gtataaagag
    10681 ttcgaaaagg tgggaaccat gcagaaaata ttcaattggc ccggtgactt ttattatatt
    10741 cgtagacttc actggacagt ccaaggtgat atctataatc atcaatatca aatccatcat
    10801 ttcaatcagc aaatcatgaa aatggataaa gcaacacttt tttcaggaga ttactatgta
    10861 ttggatgtct caaatgatga agatgctcca gtttgtattt gtattgctgc ggttttagat
    10921 tactggctct accgtaaaga caaaagtcgc gataagcctg tttcttaccg acttaactta
    10981 agttaaaaag atgtacctga aatgcggaaa tacgcatttc aggtacatct ttttagatat
    11041 tcttttgcat atgatcataa agatgaccac tgatcatccg ctgaccgaca actttgaagt
    11101 catggcgttc gtataatttt ttagcctttg gatttttttc atccacactt agaccgatga
    11161 cggagcgatt agctttcttc gccaatttag gtaatgcttc taataatcgc gaaccgatgc
    11221 cttgtcctct aaaatcttct gatacactga tagagtccaa ataccattcg ttgggaaacg
    11281 tttctttatc aatgaacaaa cgtgtagacg catctagctg atatttctct aagataggta
    11341 ccagtggctg atcgatgatt gattcttctt catctgtata accgtaagca actcctgcaa
    11401 ctttaccgtc tatttcttct acgatacctc gtgaataact gtaacgataa tttggatcag
    11461 tgattgcttc ttctaaaatc tctaaagtct tttgttcccc tgtctgcttc aaaaatggca
    11521 gttccatatc ttttagaatc actaagatca atggggcgat ttctggagca tcttcttttc
    11581 tcgcaaaacg aatcatagtt tcctctcctt ttctccattc taacatgcag aaaaagaaat
    11641 cgcaaaatcg ttattttaaa aaatcttcaa tcttttcagt aatgaaataa ccatgagtaa
    11701 cgattcttgc cgcttcagtt cggcaataag ggcagacgac tggcatttct ttatcattat
    11761 gttcgactac attattgact tcttcgtatg caaatttttt tccgcaatta gggcatttca
    11821 ttttttccat gttatctcaa cttcctttca tctttactct cttagcttac tcttatattg
    11881 taaaaaagag aagcaatatc cttcttctat gcagatatcc atacctgacg gtcctgttct
    11941 ccatctatta atcggacttt ataataagga cgttcatctt tgactagaag tgtccactct
    12001 ttcacttttg cttttttccc gtagtagctg actgcttcct tcatcgctgc ttgcggttgt
    12061 ttgaactctt ccgttttttc tatatcaaaa agatgttctg cggccttttc cgtgtcattt
    12121 cgtacggttg ttttcccagt ggatggattg actgctatga cttgtccttc acaaaaaaac
    12181 tggtattcat atgttgcatt cgtaggattc tccaaatctt tttcaactgc aaaatgaata
    12241 gcagatatgt tatgttcttt tgtttcgttt tttacgattg ctgctgcttt tttcaatgag
    12301 atccgatatt cctggtcact aatatcatcc atgtgaaaag cggcattgag cagtcccgtc
    12361 ataccggtaa tccctattac gatgagggca gcaacaccaa acatcatttt tttcattctt
    12421 cattctcctg tttctttttt ttaaagaata tcaagaaatg aacgaatgaa aaatcgaatg
    12481 aaacgaaaaa aaagtgacaa ttttgttatc tttattcgtg cagttctaat ggcaatccgt
    12541 ctggatcaaa gaaaaacgtc attttttttc ctgtaaaggt atcttctcta atcggttctg
    12601 attcgatccc taaggactgc aattctgcaa ttgtttcttc aatatcgtct actttgaaag
    12661 caagatgacg caagccacaa gcttctggat agtttggtcg ttgtggcgca ttagcaattc
    12721 caaaaatctc caattcagca cttcctagtt tcagatctaa tttatagtca tctcgttctt
    12781 tgcggtaatt ttctctgatt acttcaaatc ctaatttatt aacataaaaa tcacgtgatt
    12841 tctgataatc tgaaacaatg atggctatat gatggatcgt tgtaaggttc atatgagcac
    12901 tcctattctt cgtttaagct cggatctata ttaatctcca gttccattgt aaatgtgtca
    12961 gattgaaaaa aatagctgtt tttccttttt ataagaaaaa aggcaaaaaa aacagaaagt
    13021 aggtcctcca agacactctt cctgttccca atataataaa tgaaagtaag aaattatatt
    13081 ttcaagaaaa caaacagaaa attatcataa cacaaattat ttataattga aagactagtt
    13141 ttttttcaag aaaagcatac aatagattgt gttagtaaat acagaacaca ctacatttag
    13201 tgtcttaagg agtcctcata catgaagtca tattttcagt ttttagctca tcagctaaaa
    13261 ggctggccgc aacaaaatta ttatcttttc tttttcagtc ttggctgcca ggtcatgact
    13321 cttgtcaata gtcccatcac ttggctgtcc gttattacat ttatcggtac cacgcttggt
    13381 gtattgtgta ttttatccat caatgcggca aaatcagtca acggtatttt aggtattttg
    13441 tctgcgattt gttttattat cgttgggttt tcagcaaaga actatttaag tatcggtgaa
    13501 cagattgctt atatgatcac attggatatc cccgtattgt taagtaaaga atggaatcat
    13561 aatatggctt caaaaatccg aaaattcact ggaaaaagct ggtttatcgc aatcatcagt
    13621 actttccttg tctatcttgt ttcaggattc ctgattggtc gttatacaga tgatcctcgg
    13681 ccgtgggtag atgccattgc tttttctatt agcttgactg ctggtatcat cagtttcttg
    13741 cgttacaaca accaatatta ctggtggtta gcttcagggc ttgctcaaat ggtcctttgg
    13801 tatatttcat ttacgcatgg atctgcaagt ttggcgatgt tcgtgaacag ctctgtttac
    13861 ttgctgaatg atgtccttgc atttaccgtt tcaccttggt ataaccaaaa agaacgtgaa
    13921 cgtatgcaaa aaatggaaga agaatactac aacgcacaac aacacgcata aaaaaactga
    13981 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
    14041 atcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc
    14101 gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctcta ttttttatag
    14161 taattccttc agatactttc ttgcctcatc attgaaacgc agtgattctt ctaacttctg
    14221 ataatattcc aacgcttgtt tctgagcttc agtaaaacga taatgttttc ctttccattt
    14281 gtcgattgtc tcttgcacat ttccagattg gatatctttc actactagtt gccatttttc
    14341 ttttgagact gtacggtctt tcttatcagc agattgtata gcttctcgtt ctttacgcaa
    14401 tacttttaaa aaaccatttc tgactatccg aggaatgctg tcgaagaacc cttctgtaaa
    14461 accatagcga caatattgct tccaaataaa tagatagatt tttggttgga tgaatcgttg
    14521 gtttttcgca aaatattcca tctgagcagt gacttctggt gttcgttgac gttttgtctt
    14581 tctcaacacg gaaataaatg tttcaaaata agcattagga tgtactggca cttcttcata
    14641 tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga attcttctac
    14701 atcctttact aattcttctg caaagtcgtc aaatagttct ttttttatct gtcgcttttt
    14761 tgactgccca ttgatgtgtt cacttatttt cttgatttca ttttccaatg cttgataatc
    14821 cgctatgtaa atcggcattt ctacttggcg aaattctgcg agccgctgag ctaattttat
    14881 atccggtaaa tatggctgtt tcatccgacg attttggtac aagacaaaac agtatttttg
    14941 ccatagtcct tcaggaaaat cgatattctt ctgcttaagc cataataatt cgtctaaggc
    15001 cagatcaact tttgtcattc cctgatggat cgaagctgcc actgtcggag agacatgcag
    15061 gtgatcggca aagtggttga tgcctagttt taggatcttt ccggtttttg gcgatttaac
    15121 gatgtattgc acttttaatt ctctaccaca ctcacaatac aatttttgct ggcttcgttt
    15181 tgttcgaaaa aacggattga tcttgaagtc agaaaacacc caatctgttg cagctaaata
    15241 atgttcttcc ataaaacgag agctgattag atatcggcgg tgcttatcga ctagttcccg
    15301 ctgttttttc gttaattttg catagatctc ccaacgttga ctgcttgtgt attgtttaga
    15361 aattttgatg ggatagcttt tgctgatttc ttttggaaac cattcttcaa agacggattg
    15421 ccattcttcc acggctgttt ctttcctttc taattacaaa aagctggcta ctaacataac
    15481 gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt gttccggcat ttccggaaat
    15541 atcttcttta ttttctactg ctgcttcaac tgcataagtc aaagaagaag caatgtcttc
    15601 taaagacata gaaggcgtac ccactggacg atcgatcact tgatcgggtg agaatggcac
    15661 gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa gcaaacgata
    15721 catgatatca ttgcagacaa atgttcctgc tgtataagag atgtacgctg gtagaccatg
    15781 atcttgtaca ttttttgtca ttgctttgat tggcaatgag gtaaagtaag cggctggacc
    15841 gttttcttct aaagccgtat cgaatggctg atttccttca ttatcaggga ttcttgcttc
    15901 tgctagattg attgcgacac gttcgaacga gactgctgac cgcccacctg cttgtccgac
    15961 acagatcacg acatctggtt ggtgctgttg gatggcttct ttgactactt gactgctttt
    16021 ttcgaatacg gtcggtactt ccactttgat gatttcagca ccgctgattt catctggcat
    16081 tttttttact gcttcatagg ctggattcac tttgtcccca ccaaatggat caaaacctgt
    16141 tactagtact ttcatctgtt gttcctccgt ccgattgatt taacatatgg ttatctttta
    16201 tcataccatt ttccttgaac agcgtaaata aaggaaacaa tagtttaaat aacataacag
    16261 atcaaactta atatattctt ataaaaaaat agtgttccac tacaatgtta gaagaggttg
    16321 cgacaattat tctgtcacaa cccctctctt ctattattta aatagaatca tataagcaat
    16381 ttggaaaact agcataaata acgcaataaa caactggttt ttgatcactc cgtatttgtc
    16441 tttcatctcc agcatcgcta caggcacgat attgaagtta gcagccatcg gtgtcagcag
    16501 ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt aacgctggat tcgctccatg
    16561 actaaatacg aatggtgcac cgatcccaac cgtcatcacg gtgatggccg caaatgcatt
    16621 ccccatgatt accgtaaata tcaccatgcc aagagcgtaa atgatgatgc cgatgttcac
    16681 atttccttct ggaacgatcg tacctactgc attggaaatc actgtcccaa ctccagcgct
    16741 tgtaaatacc gcacctagag aagctaaaag cattggcaac atgcttaacg gccctactgt
    16801 gcccagcata tctgctgcat catctaaaaa tgtacttggt cgattatctc gagaaaagaa
    16861 catcaagatt agcatggctg caaaaacacc gacgcccacg ccgactaaag cacctaaatt
    16921 ggtaaataga gcaaacaaga tcgcaaagac accgatactc aaagctggaa taaaaatttt
    16981 cattcctagt ttgtctgaca ttttttccat atattctttt gatggaagcc ggctttctcc
    17041 tttgctgact ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc caccgttcac
    17101 gattgccgga agccatcttc ctcctgcaat aactacccct aagaccgtcc agaaaaaggc
    17161 tgacccataa cgatgaggat tggttttatc tgttaaattt cttactccag tgtaaatcaa
    17221 tacgagtccc atcacgatat aaaggatctc cagtaattta tcgcctaata aaatatcagg
    17281 atttgtaaaa aagtccatct tattgcccct ttcctggata atatttcttc gctaatttct
    17341 tttctttaag atagaagtag acacttgcca caactaaagc cgtgatagcc actggaactt
    17401 ccactaaagc aagctgtccc aatgttacgt catatcctag atctttcagt gttgattgga
    17461 ctagcaacgc ccctgctcct ccgatgaata gtacttggcc aaagaaccag cagacatttt
    17521 ccatagctga agccatacct tttagttctt cttcatgctt aggatggact tctaaccctt
    17581 ttgattcgat ggtccccaat gccatcggta aaatgattgg acgaacaaat ccagcaactc
    17641 caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa atatctacaa
    17701 tcataccagc agatactttc ttgaatcgct tgatcaagga agccgctgat tctttcagac
    17761 catttctttc aagcgttcct gttgccaaca taatgatgat aaaaatcgcc atcccgcgat
    17821 tgtttacaaa acttgtacct aaaagttcta acattccttg aatgccaaga ccgccagtca
    17881 aagctgtaac aactaaagca accattacga tcaatatcga gtctaattta aatgcaaacc
    17941 cgagaataac aatcaagacg cctaaaaggc taagataatt ttccata
//
LOCUS       DACCXM010000052        17340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCXM010000052 DACCXM010000000
VERSION     DACCXM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.054"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(53..598)
                     /locus_tag="IVY61_001522"
     CDS             complement(53..598)
                     /locus_tag="IVY61_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7970639.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(610..1302)
                     /locus_tag="IVY61_001523"
     CDS             complement(610..1302)
                     /locus_tag="IVY61_001523"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP7970640.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1321..1605)
                     /locus_tag="IVY61_001524"
     CDS             complement(1321..1605)
                     /locus_tag="IVY61_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970641.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1618..2181)
                     /locus_tag="IVY61_001525"
     CDS             complement(1618..2181)
                     /locus_tag="IVY61_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7970642.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2409..3071
                     /locus_tag="IVY61_001526"
     CDS             2409..3071
                     /locus_tag="IVY61_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP7970643.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3195..4391
                     /locus_tag="IVY61_001527"
     CDS             3195..4391
                     /locus_tag="IVY61_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP7970644.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4492..5868)
                     /locus_tag="IVY61_001528"
     CDS             complement(4492..5868)
                     /locus_tag="IVY61_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7970645.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5905..6771)
                     /locus_tag="IVY61_001529"
     CDS             complement(5905..6771)
                     /locus_tag="IVY61_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP7970646.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6860..7885)
                     /locus_tag="IVY61_001530"
     CDS             complement(6860..7885)
                     /locus_tag="IVY61_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7970647.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7903..8577)
                     /locus_tag="IVY61_001531"
     CDS             complement(7903..8577)
                     /locus_tag="IVY61_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7970648.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8761..9654)
                     /locus_tag="IVY61_001532"
     CDS             complement(8761..9654)
                     /locus_tag="IVY61_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7970649.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9728..11425)
                     /locus_tag="IVY61_001533"
     CDS             complement(9728..11425)
                     /locus_tag="IVY61_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7970650.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11418..12731)
                     /locus_tag="IVY61_001534"
     CDS             complement(11418..12731)
                     /locus_tag="IVY61_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7970651.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12731..16768)
                     /locus_tag="IVY61_001535"
     CDS             complement(12731..16768)
                     /locus_tag="IVY61_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7970652.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16781..17239)
                     /locus_tag="IVY61_001536"
     CDS             complement(16781..17239)
                     /locus_tag="IVY61_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7970653.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4858 a         3897 c         3012 g         5573 t
ORIGIN      
        1 gctcttcttt tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa
       61 tttctgcgcc tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga
      121 aacttgccac ggaatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg
      181 ctgtatgcaa cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc
      241 gttctcgcaa acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta
      301 tccaataaac attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca
      361 attcccgtcc acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa
      421 aggaatcttt tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa
      481 acttctttgt atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca
      541 gctttccatc ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct
      601 tagtcctcct tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc
      661 tgcgtcttcg ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat
      721 tgcacgtacg atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc
      781 ttccatttcg caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg
      841 cgagtcgaca aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc
      901 ttttgctgct ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat
      961 tcctggtaat tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac
     1021 tttgtctgat ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc
     1081 tgtattgatc accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc
     1141 taagactttt cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga
     1201 aataaataaa gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat
     1261 ttttacttct tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt
     1321 ctatataaat gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga
     1381 tttcatcaaa aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt
     1441 gattggttta ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg
     1501 ctgataagct gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg
     1561 tcgcttgcga agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta
     1621 ctttcctttg gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat
     1681 caactgatct ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac
     1741 ttcgtcttca tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat
     1801 atgaaggact tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga
     1861 cttcgcacga tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg
     1921 tccttctcct ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt
     1981 tcgaaattgt ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc
     2041 gccaggatga aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc
     2101 tacagcaaca tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc
     2161 aaattgctca ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt
     2221 ttcatttttc aatttttctc aaaaactcct tattagttta ccatagataa accttagatt
     2281 ttctctaaat atagaaaatc cttctttagt ccgatactct ttctagtagt ccgtatgcta
     2341 aaataagtat gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag
     2401 ggtttattat gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct
     2461 ttgtccgaaa caatagtttt tcttttgttc taggactttt attgataggt gtagttttag
     2521 ttttgtgggg actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac
     2581 tgactgacga acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat
     2641 tttttcgaca aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc
     2701 agcagacagc caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag
     2761 ctttattcaa agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt
     2821 atacgcatct gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg
     2881 agatcaaaaa caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc
     2941 tgtcagcttt aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg
     3001 acgtagagat atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac
     3061 aaaaggatta actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct
     3121 gtttcgagta ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca
     3181 aggaggaatt ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg
     3241 atacacttga agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt
     3301 cacaacaagc ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca
     3361 aactaccggc ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg
     3421 atgcacagtc tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac
     3481 aatcgatgtt gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg
     3541 aactgatgta tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca
     3601 tcttccagcg tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc
     3661 aaaagattgg tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc
     3721 tgctaaaaga taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg
     3781 cactgaatat ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca
     3841 ttccagaagc gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca
     3901 atgattacac gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta
     3961 gacagatcac gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct
     4021 tggctgaaaa gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg
     4081 tgatcgcatt gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg
     4141 aaacaacaaa cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa
     4201 cagcgaaaga aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg
     4261 atttgatcga aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc
     4321 gtcatgcaga agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt
     4381 taacccaata gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac
     4441 aactgttgat gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc
     4501 attcatcttg ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac
     4561 gataccgaac acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa
     4621 aatggagacg attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt
     4681 gccaaacatt tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc
     4741 catgatattc gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc
     4801 tgccacacca gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt
     4861 cataaactta ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata
     4921 catcatcgag atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc
     4981 cactgttcca tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc
     5041 agtatacgga acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat
     5101 cagtatcgaa ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc
     5161 tgccattgca acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag
     5221 cacaccggaa aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat
     5281 tcctacaatg ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa
     5341 acgtaatgct ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac
     5401 cgcaatcaag tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg
     5461 aagttcggcc cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac
     5521 agaggaaacc gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa
     5581 ttcaccaaat acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc
     5641 aaattctgcg aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag
     5701 tgacagtgct ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt
     5761 accgatgatc gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat
     5821 ctcactcgta tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc
     5881 ccattttagg ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc
     5941 taaaatgact aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc
     6001 gccaaggatg aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa
     6061 gccaacagct aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca
     6121 taataatcca cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc
     6181 aaagaagctt gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc
     6241 caaattcgcc cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa
     6301 tgctctaaaa cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc
     6361 atcttgtcgg gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta
     6421 tacataatct ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa
     6481 aattaattga aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc
     6541 atggaactgt ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa
     6601 aatccaaggg gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc
     6661 accgatcgtc attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa
     6721 accaatacta ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct
     6781 ccttttattt cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt
     6841 tttcgtttct aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata
     6901 aagtcattgt tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt
     6961 cggccagatt ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac
     7021 catttctccc agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga
     7081 tccctgaatc tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga
     7141 tgtttccgtg atccggcgta tatttgatgg cattactaag caattggttg aagataaaag
     7201 cgatccattt agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat
     7261 ttttttggat aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc
     7321 catattcttg gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt
     7381 gttctacgta ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt
     7441 caatatcgaa ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt
     7501 catggaccca tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca
     7561 ttacttcttg gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt
     7621 ctgtcctccc accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt
     7681 ttccccacca tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc
     7741 cctcaatcaa agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc
     7801 acatcacaaa gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat
     7861 ctttcaaata tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc
     7921 ctgtcctact tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa
     7981 ccgattgatg ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc
     8041 tcgcagcaat ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag
     8101 acgatattca tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc
     8161 aacattcaac gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta
     8221 agaacggcga agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt
     8281 cacaaaatca tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct
     8341 ggagataaaa atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc
     8401 atccaaaacg ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc
     8461 tgagaacact tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc
     8521 ctccatgatc aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa
     8581 aaatccctcc atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt
     8641 ggcttacctg gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta
     8701 atcgcgtttg tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta
     8761 ttatctgtta tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc
     8821 gaaaagcgtt agcactagca atatacctag aaggagtata agtaatcgat aaagatggta
     8881 tttttttgtc tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt
     8941 cactagtaat cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc
     9001 ctgcttttct ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac
     9061 gacttcataa gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat
     9121 ttcttcaaga tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga
     9181 atgtgtactc tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc
     9241 taacaaaaga tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata
     9301 gatggctccg atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac
     9361 cgcttgctca aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag
     9421 cgtctgattt ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg
     9481 ttcaagctgc ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc
     9541 tgctaaaaat ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa
     9601 ttgtaataaa agactgattt gggtttgagg tctattacct attttctttt tcattttctt
     9661 caccttctta tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt
     9721 ttgttgctta tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca
     9781 tcaaacttat tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg
     9841 acccgcgttt catattcggt actctttctg gccctggatt ttctactcct acgatctgaa
     9901 tttcttcatt atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg
     9961 gaagtacata accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc
    10021 cttgaacctc aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg
    10081 cccactcaat tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa
    10141 atattgcgta ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt
    10201 cactgcttga tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg
    10261 gctctactcc ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt
    10321 tgttatggaa atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta
    10381 aatctcctcc acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt
    10441 cttctgtcaa aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc
    10501 cttttgttaa aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac
    10561 caactgtagg cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga
    10621 cagaatcgat gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt
    10681 ctagatcaat ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc
    10741 taaggtcgct ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat
    10801 acaaatgcac atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca
    10861 ccatatcagg catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat
    10921 gagctggcga ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt
    10981 ccgtcacttg gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa
    11041 tacttgtcag tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa
    11101 tcgcttcggc agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca
    11161 aatcgtaata tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt
    11221 caccaaaatc cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac
    11281 cttttttgtg taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga
    11341 ctagttttcc gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc
    11401 ccgtgacaat cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca
    11461 gcacctaaca ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc
    11521 gggaatacgg accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt
    11581 ttgtccgttg tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca
    11641 gagactggaa ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat
    11701 tgtggttggc cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt
    11761 gcttcatcca tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa
    11821 ggcaagaaac gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca
    11881 tttttgtcat tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta
    11941 gcaggagcca tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca
    12001 gcaccttcta atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag
    12061 tatggatctc ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg
    12121 gttgattctg tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg
    12181 atatcaacat ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca
    12241 tcccgatctt ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca
    12301 accgtatctt tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt
    12361 agctgacgga tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc
    12421 atgttttcag cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc
    12481 gctccattta gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag
    12541 atagccaagc catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc
    12601 atccgtttat gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca
    12661 tgtggaagaa ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac
    12721 agctttttca ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg
    12781 ctcctgcaag cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc
    12841 ctgtagcagg caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat
    12901 ttgtctccaa aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt
    12961 ttggataata cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat
    13021 cattttctat gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt
    13081 ttctatagct gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg
    13141 aagcattgaa tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat
    13201 actctcctgc actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat
    13261 tcatactgtc aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta
    13321 cttgtagtac accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga
    13381 tgaccgtatc ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat
    13441 cagatagccg atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca
    13501 gatgcagagg ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat
    13561 agaagtatcc gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt
    13621 atgctcgata cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat
    13681 ctaatgtatg gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat
    13741 tgtaagcagt gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt
    13801 gccaattttc ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc
    13861 gcaagatcca tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa
    13921 tgattttgta ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg
    13981 gctctccgat tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat
    14041 ctactgaata gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc
    14101 ctttcgctac tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact
    14161 ctgcgccatt gattggcata ttactgcctt caattagttt attgatattc atgtggattg
    14221 gcttgaattc atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta
    14281 tgttaggctc ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat
    14341 ctttaccttc cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt
    14401 gacctaacat ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat
    14461 ctactaactg ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg
    14521 ctccagtttc atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg
    14581 tagttttact tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg
    14641 caaattcgac tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac
    14701 ggcctttatg ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat
    14761 cattctcata atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc
    14821 cgtcttgagc tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac
    14881 ctaaagaaat cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag
    14941 tcgccactac ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt
    15001 ggtacttcat gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc
    15061 cagctccaaa tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg
    15121 ttctcttgaa ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg
    15181 cattgtaata tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat
    15241 ttcgtccaat agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa
    15301 tggagaagat acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa
    15361 ctgtgttgat cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag
    15421 aagaaccatc accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac
    15481 gatagtgtac atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat
    15541 gagtaaaccc tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt
    15601 cattatcttc agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc
    15661 tcgtacgata acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa
    15721 cgattaggat tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat
    15781 agccattttt aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg
    15841 tattctcact tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga
    15901 tttgctcatg gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa
    15961 tatccagact cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg
    16021 cagcattttt agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat
    16081 ctagaacaaa aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta
    16141 gatagacatt gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg
    16201 gcagaacagg ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt
    16261 caaaattccg ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt
    16321 cgcctgcagt tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt
    16381 ttgtataatc actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt
    16441 gatagcgttc attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa
    16501 acttcgggta gcgatgctgt tcgttttcaa cttctccgct aggatagata tattcatagg
    16561 ggtcttgcgt agctgttggc ccaagacgct ccaaaatacc gttgaataac tggcgttgaa
    16621 tgcgctggag tgcttgaccg ctgggtacga ttggttcgtc cagcttttcc atctcatttt
    16681 cttcgctacg ttgaatttca gcggaatctt ctgtatcagt ttcattttct tgtaattctt
    16741 ggttatctat tctttcatca accaccactt gaagaggtag ttacccttcg ttcacatcgg
    16801 ggatcttttc tgtttcaaaa atcaatgtgc cggtttctgc ttcttttgaa taaacttttc
    16861 cgacatacca ttcttgacca tcgatcgtct cgacttttag gtcttctcca tcttcatcat
    16921 aggaggatag acctgttcca cgaacgtttt ttacggtacc aatcccggcg gctgccgcat
    16981 cgattgctaa acgtattcta ccttcattgg cactttcctt tttttcaaaa gccagatgcc
    17041 attcaagggt ttcttctttt tcaatatact tcccttcaat ttttgcatag tcctcatcta
    17101 agaatacctc atcctccttt gtttctgttg actcagcagc tacatccagg gtcgtcatat
    17161 gatagactga ctggaaatat ggtccaaata gcaaactgac agttactaac tgcaaaagct
    17221 tcaattttaa ccctttcaca tccttatact cctttcaaaa atatttgtct tctttcacaa
    17281 caatcaacaa aacaatgccc atctaattat aatttaattt aattttaatt ttaaatcaaa
//
LOCUS       DACCXM010000053        17241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCXM010000053 DACCXM010000000
VERSION     DACCXM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.055"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            34..471
                     /locus_tag="IVY61_001537"
     CDS             34..471
                     /locus_tag="IVY61_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970654.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            566..1450
                     /locus_tag="IVY61_001538"
     CDS             566..1450
                     /locus_tag="IVY61_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7970655.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1674..4841
                     /locus_tag="IVY61_001539"
     CDS             1674..4841
                     /locus_tag="IVY61_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970656.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4861..5406
                     /locus_tag="IVY61_001540"
     CDS             4861..5406
                     /locus_tag="IVY61_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP7970657.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5434..5889
                     /locus_tag="IVY61_001541"
     CDS             5434..5889
                     /locus_tag="IVY61_001541"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP7970658.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5886..6644
                     /locus_tag="IVY61_001542"
     CDS             5886..6644
                     /locus_tag="IVY61_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7970659.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6730..7470)
                     /locus_tag="IVY61_001543"
     CDS             complement(6730..7470)
                     /locus_tag="IVY61_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7970660.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7531..8784
                     /locus_tag="IVY61_001544"
     CDS             7531..8784
                     /locus_tag="IVY61_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7970661.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9106..9333
                     /locus_tag="IVY61_001545"
     CDS             9106..9333
                     /locus_tag="IVY61_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7970662.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9456..9782
                     /locus_tag="IVY61_001546"
     CDS             9456..9782
                     /locus_tag="IVY61_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7970663.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9850..10563
                     /locus_tag="IVY61_001547"
     CDS             9850..10563
                     /locus_tag="IVY61_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7970664.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10873..11723
                     /locus_tag="IVY61_001548"
                     /pseudo
     CDS             10873..11723
                     /locus_tag="IVY61_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            11844..13709
                     /locus_tag="IVY61_001549"
     CDS             11844..13709
                     /locus_tag="IVY61_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7970665.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13757..15166
                     /locus_tag="IVY61_001550"
     CDS             13757..15166
                     /locus_tag="IVY61_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP7970666.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15274..15975
                     /locus_tag="IVY61_001551"
     CDS             15274..15975
                     /locus_tag="IVY61_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7970667.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16134..16613
                     /locus_tag="IVY61_001552"
                     /pseudo
     CDS             16134..16613
                     /locus_tag="IVY61_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5756 a         2897 c         3714 g         4874 t
ORIGIN      
        1 acaatatcaa aaaatgcacg tagaagtaga taaatggagt ggttttgcaa atttctttta
       61 tttctagaag ttattcacgt atacggattt gtttttctca caaataaaaa ggcaggaagt
      121 acaatggatc atttgtatat aaaatatatt catcaatttt ccgttttggt gttaggcaca
      181 ctaattatag cggggattgt cgcatttatt attcaaggcc gtgacatatt aacagtacat
      241 caagagtttt tcagttatgc atttggtatt cctctgatcg ctataaggtc tgataacaaa
      301 aagtggaaga aacgctatta cattctcttg ctatttttgt gtttttatct gccacctaag
      361 atttttactt tattaggtat acaatgccta tcattttctt ttatttccta tatcattgtt
      421 ttaattttcc tcagtttcat agtatggatg ttttatcttg tcaaaaaata aatgaatgtt
      481 tcatgaacag tacttcatca cagcctaatt tttttcttta tgctatcgtt atattaaaga
      541 aatcaatcag aagggaaaga aagtgatgaa aaaagtttta ttagtggtaa accccagttc
      601 aggcggtgaa caagcaaagg aatttgagca attagcgata gcaaaattgg aatcggtgtt
      661 tgatgaagtg gtcgtcttgc atacaaaaaa agcaggggat gcaaaaaact ttactcgcga
      721 agcggctacg gaagggtatc atagtgtgtt tgtcatgggc ggagatggaa cagtcaacga
      781 aggaatcagt ggaatcgctg aacaagaaca tcggccaaat ttcggatttt tcccattggg
      841 tacggtgaat gacttggctc gagcattagg gataccgcta gaacctgagg aagcgatcaa
      901 tcatttttcg attgaatcag tcaagccatt ggatattgga aaaataaatg acgactattt
      961 tatgaatgtc gtcgcaattg gctctatccc tgaatcaatc aatgatgtag atgcggagaa
     1021 gaagacgaaa tttggaaaac tagcttattt catgtcagga atcaaacagt tagcttccac
     1081 acaatcctac tcgtttcacg tagaagttga tggaaagaag gaagaaattg agagcagtac
     1141 cttgctgata ggactaacta actcagtcgg cggttttgaa acattacttc caaatgcaaa
     1201 agtggatgat ggtaagctcc atttggttta tttaaaagat tcttctttga tcgatacgct
     1261 taaaacattg ccagacctat taaaaggagt agatgaatct acgaaaaatc tagcctacct
     1321 tacttgtgag caattagttg tgtcattagc tgatgaggaa gaattgacga cgaatgttga
     1381 tggagatgaa ggggataaat tacctgtgac aatcaaagta ttgccctcac atttacaggt
     1441 ttattgttaa aatggaggtt gtgaccgagg agttggcggg agaacaccgc ttgttcggga
     1501 taagggactg tgacaagact tttgtcacag cctttttttt gtacaaaaaa gaaatttaca
     1561 cctaatagaa aaatttttcc ataaaaatac atattttaaa tatgaaagta caattggaaa
     1621 tagattaaaa cgataaaata aaattgtcaa aacgtcagag aggatggtgg agaatgaatc
     1681 aatgtgtaaa aaagctgatt gcgtcgattt tgtttgtctt ccctctcttg atggtgacat
     1741 ccgtttttgc cgatgtagag aaaacaaacg aatcaacgca atcagagaat caaagttttg
     1801 acaagactac tttttcagaa gaatcgatta cttctgaaac tacagaatct caaagtgagg
     1861 cttctactaa taaaaggcaa gaagaaaata agacaagtga gacctcagaa gtagctgaac
     1921 aaacgtctga tagcagtgaa gcggcactgg caagagctgt ttcagcggat gggtacagcg
     1981 aagctgccac aacagaagaa ctggcgcagc ttctcgcaga cgaatcagta actaagatcc
     2041 gactgattca gcctttgact ttagacaggg aattagagat caaacgagat atcgttattg
     2101 attttggtgg attcgctcac aatttcggca cacaccatat ttatatcaat gagatacctc
     2161 agtcgatcga gttccagaat tttaaaggaa cagctgcaca tccaggtgga ttgacaccaa
     2221 atatcgatgg aaatgccatt atcattgctt atatgaccga tttttgggga agcagatatc
     2281 attttacggg agaaataaaa tttaccggga cacttgattt gtatgatggg tccaaattag
     2341 gactgattta cgctcctcgt gcaactgtca cattagatgg tgtttccggt gtgttggatg
     2401 tccaaccagt gaaggaagga ctgaatgccg ctccgggtaa agcctatttt tgtagaagct
     2461 atcaattgaa tgtgatcaat ggtagtcaac tttatggacc gtatctaggg aaattctacg
     2521 gttttctaga ttcaggagat gagactggta gtggaaaaac agcgccagga atccatatct
     2581 taagcggttc aaaggtctct cttgattatg atcgggcaga tggcgttagc gatgaaggag
     2641 aggcagtcga tacgctacct ggtaatgtca tgtttgaagt cagtggttcg ggaagcgaat
     2701 tttcggtaaa tacaaagatc aatattacca atgacaacaa tcgcggaatc atccaaatga
     2761 gaggttcagg atcgaaagtc actgtatcga atggtgggaa aatcaccatc aatacagcaa
     2821 ctacgggcgg atttcgcttg caaggagatt ctagtcaaat ccatgtatca agtggtggac
     2881 aaatctcggt caaaatggct ggagatgacg atcaacccgg gaacaatggg atgcgttttg
     2941 taggaaaagg cctttctttg atcgtagaag gagccggatc agttatcgac atcgataagc
     3001 agagtgggcg ttccagcgca gttcgttttg agaatggtac gcaaaagctg acagtaacca
     3061 atggaggctc attgagggta agaaatacag gtgatggcaa atcttacgtg aacagactca
     3121 atcaaggaaa tcaagcgatc caattttatg acgcttctgg ttttctagaa agtccaggtt
     3181 cagctgattt tacagtcact ggagaaaaat caaatatcga tatacgcgca gattccggag
     3241 caactgtaga tacaactgga gatattgatt taaattttac ggcaggtgaa aaaacttatt
     3301 tagtcatggt cgggaaaaca ggggaagata agaccggcat catcagtggg gatgtgttga
     3361 atgtggactt agaatctcct acctattttg atttccaaaa caaacggatt ggaagcgaga
     3421 ccaatggcat aggcggctgg gtattccaag gaaatggaga ttcttcttta aagatcgccc
     3481 attcagaaat cgctttatgg cgaaaaacag gcgcaacatt cgataacttt gaaaaagacc
     3541 cggattattt ttctgatatg acagacttat cgatcaatgg gaccgactta ggcaatttcg
     3601 tatcaagccc tagtgcaggt ttaacaaatg aatttgctca gacaggtgcc ggaatgaaaa
     3661 attataatcg aatcagtgcc aataatcagc tcccgatgat tgaatcctta agagtgccaa
     3721 cgaatgcaga taaaaaaatc tatgggcatg tcgttgttcc cgaaggtgtc gaggcaaagc
     3781 caagagatgc ttggacaggc gaagtagaag tgactttacg tattacttac gcagatacga
     3841 cgaaatcatc tgtagaaatt actgctctca cgcaaggaca aacagatgaa aatgaagggg
     3901 caggatataa cccatgggga gaaggcgaac aaggcggctt gttcgaagtt caagtaccca
     3961 atgatgagag attaaaaacg gaggatcaag tgcaagttgt tgctgctaaa aaagtaagag
     4021 gtggccaaga agtgacagaa ctggactctg ccaaaacgac agtcgatgtg attcctcctg
     4081 aacctgctaa tttaagtacc acattgatca atgcagcttc tcgcacgatc acaggtacag
     4141 gtgaagcagg agccttggtt tcattgaaaa aaaatcaagc tgttttgtca gaaacaacta
     4201 ttgatgatac aggggaattt gttctttcta tcccagaaaa tcaacttttt gcaggagatg
     4261 tattagaaat tgtattaaac gaccaagcag gagaagctac tgctaagggt gtgcagaaca
     4321 agccaagtac caacgatgaa attgggaatc acaatccaag tgatacgcca gttgtttatc
     4381 atgatgcccc gccttttgca cctgctacga agatcgtagt agaaggtggt ctctcatttc
     4441 ttgccccttc caaactcgat tttggtcaga tcaaagcaac tggtttgaca caagaagcat
     4501 ttggccaaat ggatatacag gaaaaattat ggatttatga tcagcgagaa acgaaaaatc
     4561 cgtggatctt aacgataaaa ttatctcagc ctttcacaaa tgatgcagga ttttctatgc
     4621 aggatgtcct atatttcaag aaaggagagg agtatcaatt agtgaatgaa gaagaactgg
     4681 aaattgtcag aggacaaaat tcaacgaatg tgcaagcaga ttattcagaa tatttaaata
     4741 acggtcgggc gtttaaactt gtcttaacca aagaaaatca gatagctggt aattactcag
     4801 cagagataac atggacatta gcagatgcgc cgacagaata aagatcaagg aggatgattg
     4861 atgaatcgaa tatggaataa aagtcatcga aacaactgct ctttacttat gatccttctc
     4921 cttggattca tcctttggca gacacctgtc catgcctccg aattcaattt tgcggtagat
     4981 tttgaattac cggctaatca gattgataag aacaaaacat atataaatgt aaggatggag
     5041 cctgggaaaa cacaggaaat cccctacacg gtctttaacc gaacagataa atcagtaaca
     5101 gttgagacat tgatccactc cgctacgacg aattcaaacg gtgtgatcga atatggagaa
     5161 tcaaaaagta aatttgatga aacattaagt tatgatatga aaaacttagc aaaaacagtg
     5221 ggagaaataa ctgttcctcc tcaaagtgcg gtcactgaga cactttcact gaaagcgcca
     5281 gaaaaagagc tggctggtgt ggtcgcagga gggatcacgt ttcaagaaaa aaaagagaac
     5341 gtttctaaca aaagtacagt tggtgtcgtc aatcagtttt ctcaagtgaa agcaatcttg
     5401 atttgaaata gcgaaaaaga gtcttcccca gatttgaaat taaaaaaagc ttatgcggat
     5461 caaagcgatg gaagaaatgt gattaaggcg aatcttcaaa atccaaatgc ttcttatatg
     5521 ttcggcttga ctacatcggt tgaaatctac aaaaaagaaa caaaagagcc ttacctttca
     5581 ataaaaaaag aaaaaataga tgtagcaccc aatacaaatt ttgatttact tgtcccatta
     5641 gaaggaaaaa aattagctcc gggacattat caagcggttg tgaagggtgc ttggaaagaa
     5701 aaacgctgga atctccatac cgattttgaa ataactgatg atgcagcgga ccaattgaat
     5761 gaaaaggata ttgatttaaa aaatcagcca gaaacatttg actggcgatg gctgatactt
     5821 gttggagtca ttcttattgg cttatttgct ggattattta ttttaaaaga aagaaggaaa
     5881 aagaaatgaa aaaaacaatt gcattaagta ccgttctagc tggaagtcta ttattatgta
     5941 cacaaagtgt ttttgctgag aataagagct cagccactac agaaggaacg atttctgtga
     6001 ccaaaccaga tgctgcaaat cctgaagacg ttgttccagt tcctcctgtt gatccaaatg
     6061 atcctgatac tccttttaca cctgacgaac caaatcaggg aacatccgga ttactgacga
     6121 ttgaccaagc tccagatctt gattttggaa caatcaagtt aggtacagca aaaacagtat
     6181 atgctgcatt acaaagcgga acggacagta ctggagcaca aacacaagta cagaatttcg
     6241 tacaagtaac ggataaaagc ggaaattatg ccggttggaa attatcagtc aaacgaacag
     6301 aatttattgg tacaacatct gatacgaata aactaactgg ttcaaaggtg aaattcaaaa
     6361 acgccgtgat tcgaaatagc gcaactaata gcagtggaat gccatctacc gtttctggaa
     6421 ctggcgctga aggaatcgaa atcccagcag atcaggcagt agatctagta actgcaggag
     6481 caaatgaagg gattggcaca tggatttatt cattaggaga gaatgtggaa caagggagac
     6541 aatcagtgga attatttgtt cctgtatcca attatgctgt agacaactac gtttcaacat
     6601 tcacatggtc gttaacagat gctcctacag aagaggaagc gtaaaatcat atcaacgatt
     6661 tcagggagtg gagccgattc aaggcccact ccctgaaatc tgctatgctt acgtctcagc
     6721 tcttattcat caactcgaag cagttttttg gaagtcatgg aaacgttcct ttgtcttttc
     6781 aaagatcttt cgaccaacga gtaaggagtc accgttgaaa aattctaaca cataatgagc
     6841 agaatcaatt ttctgcaatt gcgataagtt gatacagtaa gattttaaaa gtaaaaagta
     6901 agcatacttt tctaactctg tcttcaattg ttttaaagaa gtattcacta atttatcttc
     6961 tgtagttgtt ttgaccataa cttggttttt caaacctttg atactttcaa tatataaaat
     7021 ttcccgagga tacaggacga gacttccacc tttttgatca ataaagattt tatcgacatt
     7081 tacgatctgc tgttctttcc aattgatctg ttctagcact tgacgaattt gttcagataa
     7141 attcctgtct tttctgatat atccaatcgg aaggattttc cgattgatga tctctactgc
     7201 aaaattcgta taagcggtga aaaaaacaat attactatgt gcattttttt tacgtatttg
     7261 ttcggcgaca tcaatacctg tgagtgattg cttcaagtcg atatcgataa agaaaacatc
     7321 gtgattatca atagtgccca actggatcaa ctgattgatt tgagagatag aagtgagctg
     7381 cacgatattc tcgatatgat gattgaccaa ttctctgatg atccattcgc tgtgaaactg
     7441 gttatcttct atgataaaat actttttcat atgcaggctc ctattaaaaa aatatgtgta
     7501 cattttgaaa caaaacaatg gaggaataag atggtcgcaa cagtgacact ttatttgacc
     7561 tttactttga ttttagattt aggtatctat ttattctttt cgaaacaact gctagtaaaa
     7621 aacagctttg gttgtatagc tttgatggtg gtgacacttt tacttcctat ccctaaagtg
     7681 agctttcctt acctagttgg cttattattg atgtttcttt tttattttac tacgaaaaac
     7741 ctgtttatca gctgtttgat cagtaatctt ttttttgcta tcactggact cagctttttt
     7801 ctggtttttg atctgatgga ttatttagga aagagacatc ttggaacagc aagttttttt
     7861 gcactactta tcgcatttat tttctatcta ttagtaaaaa gtatagacaa acagacgaga
     7921 tttctcaact tgctttttac ttatccaact gaacactatt ttggaggtat ttgtatttta
     7981 ttaagctggg gcgtgattta cttggtattg ggaagtgttc acttccgctc atctaattat
     8041 ctgcttctat ctcttttgtc gatagtcatc agtttttttt ctttgttttt ttccattatg
     8101 ttgatcagtt tccaagtaaa agaaagacaa catcaggaat cgcttcaact gtatcaaata
     8161 aatgaagaat attaccatca tctagaggaa ttcaaacatg attataaaag tctactgttt
     8221 tcattaaaaa ttactcttca ggaaaataaa aaagaaacgt tggaattttt agaaatatta
     8281 gaagatcatt ctgcagctat ttttgaaaaa ccgcaaatac aacagctaac aaatattgta
     8341 tccccagctg tcaggggtgt ctttttaaaa tttctagaaa aagcagaatc ggagaaaatc
     8401 ccgatccaag tagttattcc tgtcgaagtg aaaacaatcc cgattgatct ggttgttttt
     8461 attcgctgtt tatctatttt tatttctaac gcattcgatc atcgtgtagc cgatcagagt
     8521 ccgattacga tcaatctgct ggaagaaaaa gagggatcac gttttagtat agcgaacaag
     8581 gcaaatccaa cgaatttgac gctatcggag atggtcaaaa gaggaaaaac ctctaaaaaa
     8641 aatggcgggt taggtctata cagtgcgaaa aaaatgttgg atgtttacga aaatgcagaa
     8701 ctaaaaatcg aattttcaaa acagaatcac tatttttttg tagaaatgta tctggcatct
     8761 agaaagagca aatcagccta ctgacttact gtcaaaggat aagtttgagg aaagtctaca
     8821 cggagataga gacaacaaca cagcatattc aaaagaaaca gaagcgtgat gaccgttgtc
     8881 acccttctgt ttcttttgct atgtagttaa acaaagtagt tgacacttcc gctacttagt
     8941 attagtacga aagtatactt catgatacta agtagataag aagtgaaaag cggaacccac
     9001 gatcgtcaaa atgctaactg ctttgcttac accagataaa gggaagagca atatcgctta
     9061 ttttaggcgt ggagaagcaa gcaaatccct acgatcacaa aaaaaatgct gacccagcag
     9121 ttaagagagt tagaacaaga tgaaagtatc catcgaaaag cctataacca agtcccgcct
     9181 aaagtagagt actcgctcac cgaagaagga aaatcattaa gagagattct agtagcgttg
     9241 tctgtttggg aagaagaacg tatcgaaaaa caaaaagcct caagggaaga agacacgcta
     9301 ctgcatacac atggtggcta cctaaattat tgattttcta tgacatttat tttaaataaa
     9361 agaaaacagg agttttttta aaagcatcat ctatttttta tgtaaaaaaa tggtatgatt
     9421 aatcaagtaa gaagtgattg gaagaaggca agcaaatgaa tagtatggaa aaagtcaacc
     9481 agtttagaga tgaacgtaat tggcgacaat ttcataacga aaaagatttg gctatctcta
     9541 tttctttgga agcatctgaa ttgttagaac tttttcaatg gaaaacgccg gaagaagtaa
     9601 aagaaacaca attagaacga atcaaagaag aactggcaga tgtattgatc tattcataca
     9661 tgatggcaga caaccttcac ctggatctag atgagatcat tgaagaaaaa ttgatcaaaa
     9721 ataatttgaa atatcctgta gaaaaaagcg caggtgtacg aaaaaaatac acagagttgt
     9781 agaaatgtgt cattcggctg ttggaaagag caaaagaaaa acagtaaaat gagtctggag
     9841 ggagagaaaa tgaaaaaaaa aggaaaagca aaaagatggc tagtcaatat cctcttgttc
     9901 ctcttgttat tagtaggact agctttgatt ttcaatgaac agatcaagga ttattttgtc
     9961 agagagacag gagataaata tgctatagcg aatgtaacaa aagaagatct gaaaaagaat
    10021 aacgataaag atgtcagttt tgactttgat gcggtagaac caatgacgac agaaggggtc
    10081 atgaggtcgc aaatgagagg aacagatcta ccagtgattg ctagtatcgc agtcccttca
    10141 gtttcaatca atttaccggt tttcaaaggc ttggataata cgtcactact ttacggagca
    10201 ggaacattat ctccagatca agaaatggga aaaggaaatt atgccttagc aagccatcgt
    10261 gcaacgaacc ctgaattgct gtttacaccg ctagaaaatt tagagatggg tgccaagatc
    10321 tatttgacag atttagaaaa tgtgtataca tacaaaacat tttttaaaga aaaagttgct
    10381 cctacagata cccaattact gaatgaagtc gagggaaaag aaattgtcac actaattact
    10441 tgtggcgaca tggacgccgt cacgcgacta gttgtacaag gagaactgga aagtgttaca
    10501 tcgataaaag atgcgacaga tgatatgaga tctgctttca atctagagac aaaaacattt
    10561 taggatactg ggacataagt cgttacagat taaaaacagc caaaggatat gaatcgataa
    10621 ggaaaaacca atttgctgaa aaaaagagaa acgcaaagag gtgcatctct gatttggttg
    10681 ttttctagat agacttgtgt tccagttttt ttatttttta tatgaaaagg cttgcataaa
    10741 taaaaattcg atggtatgat gattttgaat aagaaatgga ttgttactgt acggcaggca
    10801 aaaccagaat tcagctaaga aacagtaggc gaattctagg tttttttatt tttcttgaga
    10861 gggcgaagca aaatgaaaat tgataaaatt ttgaataaca atgtcgtcat ttcgaaaaat
    10921 ggattcgggg aagaagtcgt ttgtatggga aaaggactag cttttcaaaa aaagattggg
    10981 gacgagattt cgccagaagc ggtccaaaaa gaatttgttt aagagattct tttgctaaaa
    11041 accaattcca gcagctgatg gctgatattc cggcagagga gatcgaatgg gtcaaacaag
    11101 tagtcgaact ggctgaagat aaactaaaag ttgaattttc gcctaatatt tatctgacat
    11161 tgaccgatca tcttcattat gcgatcacac gagcaaaaga aaatatccaa ctgcctaatc
    11221 cattattatt tgaaacaaaa aaattttatc cgaaagaata tcaagcagcg aaagaggctc
    11281 ttttgttcgt gaaacaaaag acgggtgtgg aactttctga ggatgaagcc ggttttattg
    11341 cctttcattt tgtgaatagc caacaaggaa acgaagacat gcaagtgacg atgaccgcta
    11401 ccacaatggt aagagatata ctgagtatca tcagtaaatt cttcggcatc gtgttcgatg
    11461 aagaatcttt gaattatcag cgtatcatta ctcatctgca gttttttgcc cagcgttatc
    11521 tcaaagggga aggatctgac gagcaagatg aatttctcta cgcattagtt caaggaaagt
    11581 atccaaaagc gtttcattgt gtcgaacgga tcaatgagta tcttcgacaa acgcaaaaac
    11641 aggaaatggg gatcgcagag cagatttatt taacgattca tattcaaaga gtagtaagtg
    11701 agaaaaaaag catccaatca taaggattgt tactgtatgg caggcaaaac ctggattgaa
    11761 aaaataggtt cacgaatgtt tgtgactgtt tttttcaatc tgggttttta ttttttaaat
    11821 aaaaaagaaa gaaggaaaaa acaatggata atcaagcagt tggtcgtcgt gtatgggaag
    11881 cggtaggagg acaaaagaat gtcaaaagtt tagtacattg tgctacgcgg ttgcgattca
    11941 gattaaaaga tgaatcttta gcagatacgc aaaagttaaa agaagacccg gacgtgatcc
    12001 aagtcgtaca aagtggcggt cagtatcaag tagtgattgg cagtaatgta gcagatgttt
    12061 atcaatcgat cgtggatgag gaaggtcttg cagtttcgga ggatggaaaa gaagagtcca
    12121 aaaatatttt gaatcgtctg atcgatatta tctctagtat atttacacct tttctagggg
    12181 cgatggcagc cgccggggta ttgaaaggat tcttatcatt ggcaacggtg atgggctggc
    12241 tgacagctga tagcggtact tatcaaatat tgtttgctgc agcagatggt gtctttacct
    12301 tcttacctgt catgcttgct tttacagcag ctaaaaagtt caaagccaac cagtttttag
    12361 cagtagcaat tgctatggca cttgtgtatc cagctatcac agcagttgct ggtgcgggag
    12421 aagctatcag tttctttggg atcccagtga ttctttcacc atctggttat acttcttctg
    12481 tcataccaat cattctagcc gtatgggttc aaagtaaatt agagccattc gtaaaaaaag
    12541 tgattccgca atttctgcaa atgattttag ttccgcttgt cgttttagta gtgatggttc
    12601 cattaacatt tcttgcttta ggaccaatcg ggacggttgc cggtaatgct ttaggcggtc
    12661 tcttcaactc gatttatgga tttagtccaa tcgtagcagg tctaatcatg ggaagtttat
    12721 ggcaagtatt cgtgatgttc ggcatgcatt ggggattcgt accgatcatg tttttgaata
    12781 tcgaacaata tgggtttgat gtattgatgc caatgcttct tccagccatc ttggcacaag
    12841 gtggggctgc tttagctgta gcattgcgaa caaaagatac gaaacttcga gcattaggga
    12901 tttcttctac agtgacttcg ttatttggaa tcactgaacc aactgtgtat ggtgtgacat
    12961 tacctttgaa gaaaccattc attgccgctt gtatttctgg aggtattggt ggagctatta
    13021 tcggattttc cggagtaaaa gctttctcca gcagtttggt cagcttattg acgattccga
    13081 ctttcattaa tacggtggat ggtgtagagt caaatgtgac agtagcagta atagctacag
    13141 gaattgcttt tgttcttgca ttcgtaggta cattgatttt aggatttgac gaacaaacac
    13201 aagacaatca attagaaaac aaacatgcga atgcaggaga accgattact tcagcacggc
    13261 ataccttaaa aagtccttta acgggtaaag tactgccatt gagtgaagta ccagaccaag
    13321 ttttttcttc tggcgtgatg ggaaaaggta tcgctattga tccagaagta ggagaactag
    13381 ttgcacctgc agacggagag atcacgacta tttttccaac tggacatgct gtagggatca
    13441 caacgactga cggagcggag attttgattc atatcgggat ggatacagta gaattaaatg
    13501 ggaatggctt tgagatctta gtcaaacaag gggatctcgt caaagcagga gatttattga
    13561 ttcgttttga catcgaagca attagagcag ctggctatag cgtaatcact ccagttgtca
    13621 ttacgaatac tgatgcattt gccgatatac ttgaattaga ccaaaaagag attattgcaa
    13681 acgaagatgt tctggcaatt gtaaaataag gaggaaatca ggatgcaatc acgtttttca
    13741 gaaaaatttt tatggggtgg agcaactgct gccaaatcaa ttagaagggg cttatgatcg
    13801 agacggaaaa ggactatccg ttgcagatgc tatgcctgga ggtaagcagc gctttgcgat
    13861 tatcggcagt gaagaatttg actggacgat tgatcaagaa aaatatattt acccgaatca
    13921 tcgaggaatc gatcattacg atcgtttcaa agaagatctt gccttgtttg cggaaatggg
    13981 attcaaatgt tatcgttttt cgattgcctg gacacgaatt tttccaaatg gagacgaagg
    14041 tactcctaac gaagcgggat tagaatttta tgatcagctg attgatgagt gcttaaaata
    14101 tgatattgaa cctgtgatca cgatttctca ttacgaaatg cctcttcatt tggcaaaaga
    14161 atacggtggc tggaaaaatc gaaaactgat cgacttctat gaacgatttg ctcagacagt
    14221 attagagaga tacagcagta aagtcaaata ttggatgact ttcaatgaaa tcaattcggc
    14281 ctttcacttt ccagctttaa gtcaaggatt agtaaaaagc aatggagcag gagagtacca
    14341 aaatattttc caagcatggc ataatcaatt cgttgcaagt agcaaagccg taaaaatcgg
    14401 ccacgaattg cgttcggata tccaaatcgg ctgcatgatt atctatgcaa caacttacag
    14461 catcgatgcc aatccagtca atcaggcagc aacaatgatc caaaaccaag aattcaattt
    14521 tttctgcacg gatgtccaag tacgtggaga ataccctgca tacacagcgc gtacccataa
    14581 aaaatatgga gtcgatccgg aaaaattaga gcaaacggaa aaagacttcc agttgctaaa
    14641 agaatatcca gtggactaca tcggatttag ttattatatg tctacggcaa tcaatgagac
    14701 tgatccacaa gcagcaactt cagaagggaa cttgctggga ggagtaaaaa atccgtttct
    14761 tgaggcaagt gaatggggct ggcagatcga tccagaaggg ctgagaatcg cattgaatga
    14821 attatacaat cgttaccaga aaccattatt cattgttgaa aacggtcttg gagccattga
    14881 tcatatggaa gaggatggca cgattattga tgattatcgg atcgattatt tacgtcgtca
    14941 tatcgaagcg atggctaatg cagtagcaga cggtgtagat ttgatgggct atacgccttg
    15001 gggatgtatc gatcttgtca gtgcatcaac aggagaaatg agtaaacgtt acggctttat
    15061 ctatgtggat ctagacgatg aaggaaatgg gacactgaat cgatcaaaga aaaaatcttt
    15121 tgattggtat aaagaagtaa ttgcctcaaa tggcgtaaac ttgtaattaa agcagctgaa
    15181 tgaaaaagaa cacacttctc acatgtgatt tgtgttcttt ttttattttt tatgtgctat
    15241 tcttttagta atagtaaagc aaagggtgac gttatgaaaa ggacacgtgt actatacttg
    15301 gaagtcgcag ataaaattaa agaagatatt ttctcaggaa aatatccagt cggttcgatg
    15361 ttgccgacag aaagtgaatt agaagaactg tttcaagtca gcaaaatcac tgtccgaaag
    15421 gcaatcgaac tgctggcatc agatgaatat gtcgcaaaga aaagtggtcg tgggacgacg
    15481 gttctaagca atcgtcctta caatcgatta tccaaagcgg caagctttac gcaaatcttg
    15541 gagaactccc gatatgaagt ccggaaagaa acgctggatt tgcaattggt ccatataaag
    15601 aaggaagatc ctttgttttc ctttttcggt gagcaagctg tttgttttaa gcgcttatat
    15661 tatttgaaca atcagcctta tatttatttt gttcattatc ttccacaaga aatgatgcaa
    15721 ttcagcagag aagaatttgg caaagaatct ttgtatcgtt tgctgacgaa aaaaggatat
    15781 actattgact cattttctga tgatttctcg gcagtatttt taacagaaga agaacaagga
    15841 attctgaaaa cagacgcaca gatcggcatc aaacggatac ggaaatcgtc tacgcaaatt
    15901 ggggaaatga ttgaatattc ggaggcggtc taccatacgg agctgcatcc ttaccatatt
    15961 gattatgaaa cataaataaa aaaagaggtt gtgaaaaagc tgccctgcat caagtaataa
    16021 gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttaggctta ttaccgcaga
    16081 tacacggctg tttctggatt accatttatt ctggataagg gggtgtggca cgattttcag
    16141 aaggaatcta tgaaccaatc actgtattga ttttagcttc gatggtaacc ccattcggct
    16201 tgacgatcgc ttatttctta gggaaaatca ttcgtaaaaa tatactcaat cgtcaagaaa
    16261 tcgatacatt gaagaccgct tttcctatgg ggatctgtca gatcacagag ggatgtttcc
    16321 ctatcgtatt gaacgacttg gcacgcaatg tcatagcaac gggagtcggt gggacagtag
    16381 gtggcggatt aagcatgttt tggggcgcag atagccgtat tccggcatcg ggaatgtttg
    16441 cagtcgctac gatgaccaga ccatgggcat tcattggtgc attgttagct ggttcttttg
    16501 tcacaggaat aaccatttta ttattgaaaa aaccagtaga tccgaatgca gaaatcatcc
    16561 aagaagaaaa agaggaagaa gacatatctt gggatgattt gacaattagt taggagaggc
    16621 agaatggatt acaagaaaaa agtacaatag atgtaccttg agacaggaat ggtatgaaag
    16681 aatcagagtt agaaagaatc gaatatacag attttggttt aggagcgatc gaaactgtcc
    16741 agagcatcgg caccctcctt gaaatgacga tgaaggaaaa atgagaacct ttcgttgtcg
    16801 aaagggtctg gtatacctta tgtcgaagga aaggaaacaa aaaaactttc ctgtactatt
    16861 ccaaaaagga aagaaagtta ttatacagta aaaaaagaaa ttattcttca tgctagagat
    16921 cagtacacgt tcgagcccaa tatcaaacac tagttccagg caggcgcaac aggtgcggtt
    16981 atcactgaat tttcttccag cagtgacgat tcaagtgata tttttacaaa tccagctatt
    17041 tatcgttaac aaaaagattt ttaggagaag ctgatgaaca aaaatgatca aattttgatc
    17101 gtttagttcg tcggcttttt tatttagaga aacatcattt atcagtgaaa cataacacga
    17161 gaacaaaatg attttttcgt taataaaaga aacaagtatt tgttgtttaa aataatacac
    17221 gttattggca tataggacga a
//
LOCUS       DACCXM010000054        16798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCXM010000054 DACCXM010000000
VERSION     DACCXM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.056"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(36..749)
                     /locus_tag="IVY61_001553"
     CDS             complement(36..749)
                     /locus_tag="IVY61_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7970668.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            924..2348
                     /locus_tag="IVY61_001554"
     CDS             924..2348
                     /locus_tag="IVY61_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7970669.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2647..3354
                     /locus_tag="IVY61_001555"
     CDS             2647..3354
                     /locus_tag="IVY61_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP7970670.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3341..3664
                     /locus_tag="IVY61_001556"
     CDS             3341..3664
                     /locus_tag="IVY61_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7970671.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3753..4790)
                     /locus_tag="IVY61_001557"
     CDS             complement(3753..4790)
                     /locus_tag="IVY61_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7970672.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5111..6094)
                     /locus_tag="IVY61_001558"
     CDS             complement(5111..6094)
                     /locus_tag="IVY61_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP7970673.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6509..7279)
                     /locus_tag="IVY61_001559"
     CDS             complement(6509..7279)
                     /locus_tag="IVY61_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7970674.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7272..8207)
                     /locus_tag="IVY61_001560"
     CDS             complement(7272..8207)
                     /locus_tag="IVY61_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970675.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8523..10172)
                     /locus_tag="IVY61_001561"
     CDS             complement(8523..10172)
                     /locus_tag="IVY61_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7970676.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10289..12892)
                     /locus_tag="IVY61_001562"
     CDS             complement(10289..12892)
                     /locus_tag="IVY61_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7970677.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12904..13953)
                     /locus_tag="IVY61_001563"
     CDS             complement(12904..13953)
                     /locus_tag="IVY61_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP7970678.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13979..14293)
                     /locus_tag="IVY61_001564"
     CDS             complement(13979..14293)
                     /locus_tag="IVY61_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP7970679.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14309..14755)
                     /locus_tag="IVY61_001565"
     CDS             complement(14309..14755)
                     /locus_tag="IVY61_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7970680.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14756..16732)
                     /locus_tag="IVY61_001566"
     CDS             complement(14756..16732)
                     /locus_tag="IVY61_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP7970681.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT         4990 a         3412 c         2762 g         5634 t
ORIGIN      
        1 aaaaaataag ctctgaaaaa ataattttcc cgagcttatt tttcaacaaa aacagcttca
       61 aaaacaaagt cttgatataa atgtctcgat accgaatact cgaaaggttt accagacgat
      121 agagcaacaa ctttgtctat ctcaatcata aattcacctg gatctatttt taatattttt
      181 gcgtcaaatt cattcgcagt atctcctttg atacgtatgt gcgaagtctt tatttccaga
      241 cccagttcat ttgtgatata agcatataca gatttcttta aatgttttgg ttccaagcct
      301 ggtatgactg ctaatggcat gaatgtttgc tcaatcgagt atggttgaca atcaataaaa
      361 cggcttcgta caatattata tacatagtct tttacactga tacttaattt atcagctact
      421 tttttcgatg gaagttcaat tgagaaagta tggatttctg aatctacgtg ttcttcacca
      481 accacgctaa acaaaccttc gtttggacca agaactcgtg agttatcaat tggtgttctg
      541 acgacttcag tcccataccc acttctactt cgcaaaactc cttcggaaac aagtaagtct
      601 acgcctttct ttactgttaa cttgcttgta tgataaataa ctgccaattc atctcctgta
      661 ggaagtttct caccaatttt atactctcca tttacaattt ttttctttat atctaagtaa
      721 atttccatat acttcgcaat ttttttcaat ggtgaccctc caatcaaaaa catatcttca
      781 tctatacttt aacataaata aaccgataaa atccacatca aaattataca cacaataaaa
      841 aagatatata tatttaaaca aaactattta ctttaagttg taagcggtgt tatcattaag
      901 tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa gctaactttt ccagatgatt
      961 tttggtgggg ttccgcttgg tcagctgagc aagcagaagg aaaaggagaa acaggaaaag
     1021 cagagactat ctgggatcgc tggtttaaag aacaacctta ccggttttat caacgaatcg
     1081 attcctctat tacaaccgat cacatccatc aatataaaga agatatagaa ctaatgaaaa
     1141 aaactgggca taactccttt cgcgtttcta tttcttgggc acgaatgttt ccaaatggcg
     1201 gtaccggaaa agtaaatccc aaagccattt ctttctatcg aaaattattt tccgaaatga
     1261 ataataacgg aatcaaagta tttgccaact tatatcattt tgacatgcct gctactcttc
     1321 aagataaagg aggatgggaa tcacgtgaag tagttgatgc ttatgttcat tacgctgaaa
     1381 cttgttttaa agaatttggt gacttagttt gtcattggtt taccttcaat gaaccacttg
     1441 ggcctatttt aggtacttat cttgaagatt ttcattatcc aaacctcatt gactttaaac
     1501 gcggagcgca agcagcattc ttcactatac ttgcacacgc aaaagcaata caagtattta
     1561 aagaactagc tcttcctagt aaaataggcg ttattctaaa tttgagtcct acttttcctc
     1621 gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc tgatttattt tatacgcgga
     1681 gctttttaga tcctatggtt aaagggaatt tttctaaaaa gcttatcctg ttattacaag
     1741 aatataatca gcttcctgaa gattatacga aagaagatct tgtatgtatc tacgaaaata
     1801 ccgctcaaat tcttggacta aactattacg agccacgtcg cgtcaaatct cgttcttcta
     1861 tggtggatac atctagtcca tttttaccag agtggttttt cgaaccacat atcatgcctg
     1921 gacgacgaat gaacgaatac cggggatggg aaatttacga aaaaggaatt tatgatttgt
     1981 gcatggatat caagaaccat tatggaaaca tcgaagcatt tatctcggag aacggaatgg
     2041 gcgttgcgga tgaagaacgt ttcatggatg atacagggca aataattgat gattatcgga
     2101 ttgagtatat caaagatcat cttgcttact tatggaaggc aataaatgaa ggatgtaaca
     2161 ttaaggggta tcatctttgg acatttattg attgctggtc atggatcaac gcctataaaa
     2221 accgatatgg gctggtttca ctcgacctta ctactcaaaa aagaacaatt aaaaaaagtg
     2281 gcgaatttta caagcaatta agtgatacaa atggatttag atacgatgta gacttgttag
     2341 ttgaataata ttctaaaatt ccacttgaag tgaacacatt agaaaacaag aataatgaga
     2401 aataccaata acaaatactt tttcttcata cttcatttat acttgtcagt ataagagact
     2461 gtgacaaatg tagtgatcct cagaagttag gtctggaaat ctaacttgag aagatttttt
     2521 taaactacgt ccagtctttt ttatttgttt aaaattgaag ttgacaagtc ttcattaaaa
     2581 atttagaata ttcacaagtt tgacgacaat tatgaaaatt tatgtcgtgc taaaggagtt
     2641 ttgttattgg atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt
     2701 tttggttata tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cgggtttcat
     2761 ccgctggtcg tcacgttgat gtccctgctg atctatgctg gttctgccca atttatcacg
     2821 gtcagcatgc ttgctagcca tagtccactg ctctccatcg ttctctcgac ctttctagtc
     2881 aattcccgaa tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg
     2941 cttcaaaatc tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat
     3001 aaattgaact atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt
     3061 tcttatttga cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact
     3121 gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat
     3181 cttcaagtca tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc
     3241 actttgattc tggtatatgt cggactgatt tttatcccaa gtaacttagt gattttagtt
     3301 gtaacactga ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac
     3361 attgctgact attttaggtt gtactttagc aacttggctt tctaggatcc taccatttgt
     3421 cttactgaaa aaattcgatt tgccgcaacc gttgcttgag tatttaagtt ttgtcccgat
     3481 cgtcattatg tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc
     3541 gcagatcaat ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc
     3601 aaaaagctta ttggtgatcg tcttggtagg aattctttcc ctttcgttga ttcgcttatt
     3661 ataaataaaa aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa
     3721 gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg
     3781 acttttccta atatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca
     3841 ttcatcccat ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa
     3901 gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta
     3961 gcgaacatat actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac
     4021 ggctctttga tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgtttgatt
     4081 tgatcaaagt agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct
     4141 aattcacgaa aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt
     4201 tttttcagcc attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa
     4261 tgcgctagtt gattcaaaat cgaaccgacg cccccagctc cgttgatcac taagattttt
     4321 ttcccgcggt tggcattttc ttctggagtc agaccaaatt tttcaaataa caattcataa
     4381 gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat ctgaaagatt ttttggtgca
     4441 agagcgacaa tacgttcgtc aaccagttgg tattcttgat tgcttcctgc tcttgtcgtt
     4501 gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt gcacttcatc acctacagca
     4561 acaactttcc cgactccatc aaaccctaag atacgtaaag catttcttat cccgttatct
     4621 tggcgaagtt tcgtatcgac aggattaaca gatactgcgt tgacttttac cagaagatcc
     4681 cgtgaactgg gtacaggtat ctgttctttt tcatcaataa agctttttgg atcatcgatc
     4741 gacaaccctt cataaaaacc gactaccttc attaattcct tttctgacat acgaattcct
     4801 ccttagcttc atcaagttgg agcatccata cagttttcat ttcaatatat atagtatgct
     4861 atttgttcca cttttattca tcataattat tctcttactt aaagtaaact atttgcactt
     4921 ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca acctctttta aacggtgttc
     4981 aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa tatgaggagc tatttttgtt
     5041 tggctgttct tattacttga tgcaggacag ctttttcacg acctctaatt cacggtgttc
     5101 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtgtctaaga
     5161 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
     5221 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
     5281 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
     5341 tttctgtatg ttccctcggt tctttttcaa atgcataagg attcttgaaa atcccccggc
     5401 caatcatgat gccatctacg ccatattttt cggccaattc ttctcctgtc tggcgatctg
     5461 ggatatctcc gttgatcgtg atcagtgttt gcggagcgat ttggtcacgt aaagcaatga
     5521 ttttagggat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
     5581 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
     5641 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagccg
     5701 cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa gccactgcct tttccacgtt
     5761 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
     5821 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
     5881 tcggctgttc atcttctgta aaggtcaatc ttcctcgtac actgtctttt ccatctggat
     5941 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
     6001 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
     6061 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
     6121 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
     6181 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaagactgt gacaaaaatc
     6241 ttatcacaat tccttttccg gaataaatgg tgttcaaaag cttgcatctg cggtaataag
     6301 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
     6361 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
     6421 agctcaacaa acgacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
     6481 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
     6541 aataccaagt aacggcgtac aaaacaacga tgaatagcac aagtacgatc atcgatgttg
     6601 ctactggcac atcgattgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
     6661 tcggattgac tttcgaaaca gcttgccaaa atggcggcaa catagagatg gcatagaaaa
     6721 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcgtcat
     6781 aagactgagc aaaaatcccg ttgagcagtc ctgctaacga aaacaagatt gccgtcatca
     6841 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
     6901 tgatcgttac gagagagccg acgagaatac tgcgacccac tccgccaatc acaaagcccc
     6961 aaatgatcac atgcgtagga acaggcgcta ccaagatttc ttcgatgttt ttctggaatt
     7021 tttgagagaa gaaggacgaa gaaacgttgg catacgaact agtgatagca gacatcataa
     7081 tcaaaccagg cacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgaccac
     7141 cgatcatttt tccgaaaata acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg
     7201 tttggaccca gatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
     7261 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaaa
     7321 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
     7381 gtcagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
     7441 tgttcatctt caaatgtaca agcgtatcct tcaatcacag gtttggtctc atatggcgct
     7501 aaatcaaaga taaatgtttc aaattgcagt ttggatagaa gctctttcat actagtattc
     7561 tcgatcagtt cacctgattg gatgatcccg atgttgcgac aaagcatttc agcttcttct
     7621 aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg gagaaactcc
     7681 cacatctcgc gtctcaattc aatatcgact cctgctgttg gttcatccaa gatcagcaaa
     7741 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
     7801 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
     7861 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
     7921 tggaccgttt caaagggatt gaaattgaac tcttgcggga ctaatccaat ctgttgcttt
     7981 gctcggacta gatccgtatc cagatcataa ccgaagacat tcacttttcc agatgtttta
     8041 ttgactaaag aagtaatgat tccgatcgtt gtagacttcc cagctccatt tggtcctaat
     8101 agcgcataga aatctccttc tttcacggaa agatcgattc ctcgcaatgc ttcgacccca
     8161 cttccataaa cctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
     8221 ccttatagac atgattcttt attgtatcat ggaatacagc agatgaaaaa acaacttggt
     8281 tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc taatgaaaga catcaatcgg
     8341 acgaagtcag acaacctttt cagaaaaaac aaatcgaaga taaaggatta ctattcatat
     8401 gtgcaacaaa aggagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
     8461 gtataaacgg cggaagcaaa agccaagatt tttgatccaa taaatacttt cgatcacttc
     8521 gcctatccca atacatgcca ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg
     8581 gtttctacta aagtctctgg gtaatccaaa actttcagca aggcaatcgc atttcgggtc
     8641 cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt
     8701 tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt
     8761 gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa
     8821 gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat ctacgaagca gtagcagcgt
     8881 tctttggaag ccacttggtc caacaaacgt ttgatcgatt tgatttctga aacaaaataa
     8941 ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc
     9001 agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct
     9061 atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc
     9121 tgccagttga ccggattcac taccgcttct tttaacaatg gatgatagat atctttcgct
     9181 gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg gcatataagt acggtaattc
     9241 aatattgcag cggctacttc taattctccc agcaattccc acagcttgat ggcttcagtt
     9301 gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg
     9361 atgttgacat attcaaaaat caactctgct tcactcccac tcttcatacg aaatgaaaaa
     9421 cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttc ttgaagtggt
     9481 gtcggcagtt ttgctagctg attggcggct gaaatcgttg aaaccaaata actcatgcta
     9541 ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaac
     9601 ccaccgattg ccaaatagat tcctagtact tgtcctaaaa gaagcaaacc caaacccacc
     9661 agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca
     9721 tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat
     9781 tggatttttt ctctcagttg aggattttcc tgataaaaag caatcagttt ttctaactgc
     9841 tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca acgcttctga acctacacta
     9901 gaataggtac tgttgattcc ttcgaatact tcgaacatat ccaggtcata ccaagtaaga
     9961 tcatcgattt cgctatccca tgaacggaac ttcttctccg tgagccatgc ttccttcaag
    10021 ctttcttctt tatcaaatct cggttgataa ggaatttttt cccatttgct acgaactgtt
    10081 tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta ataaaataac agctgcaata
    10141 ataattaaaa cgataaattc tctttgaccc atatccctct ccgttccttt atttgttagc
    10201 tttagtatag cactgttgcg ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa
    10261 aggtgttttt ttcttgtata ttacatattt atctctcatt tatcaaaatt gacaaataat
    10321 catagggagg aattgttgtt aattcttgtt tattctccag tgcattgaca actgtcccaa
    10381 gggaaaggat agcctcaggt actatttttt cttgttctgt tggattagct aagcgaagaa
    10441 caaatccttg ttcatgaaaa taagaaggat aaatcgatga tacaattaag tcttcaggta
    10501 attcaatcaa tgaaaattta cgaggaacat caagttcatt ttgtaatggc caaatcttgt
    10561 tatctaacct gtgaataaat acattcagtt tctgcttttg ataagaaata gattgttcta
    10621 accgtttttc tgcctgtttt gccactttga actcattaaa tcttccttct tctacattaa
    10681 atccaaaagt gactttatac tcacctatct cttgagctag cggtgtttcc atcatgacat
    10741 gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc
    10801 caagttcccc tgttgttgaa aataaggtta tgattaattg attttctata cgttcatact
    10861 cttttaaacc atctgcataa aacgtaaaat aatgatcatt ttctgccaca gagacactct
    10921 tatcgaatat ttctaaatta acaggtttct ctaccatagt ttctgaccaa ttcggaggaa
    10981 tgacatcagg ggtattttca ataaatccgt tttgaatttg tgcaattgat ttttcagatt
    11041 tatctaaaac attaattgct aagcgcatac gatgcgagta aacagtatta tgaattttga
    11101 gtgtgccttc aacgatttga ctttctccca gactgaacga tatttcataa gatatttctt
    11161 ccatttcggg tttttcagat aaacgatctt ttaaatcttt aggaagttga gcttttccgt
    11221 aaacaactaa tgtttcttgt aaagaatctt tatatacttt agctgtttct aattttagag
    11281 atagttctgt atctccttct aatggtgaat aatcatacgt atcgccatca tttgctgaat
    11341 cgatcaaatg aacgaaatca agatattgtc tatctccaac tatcaaattc aattgcccat
    11401 ctttaaaaga aagtttatat ttattgttac ttatctcagt tttgtctatc tcgattgctg
    11461 tttctaattt ttcattattt ttctcaaatt caacaacagt gtatcctagt gctggcacat
    11521 ttacatttat ttttatgtca agttcataat aagctggttc atcaaaatat tcgaaacctg
    11581 tagcaatcat ccgataagca tttttccgtt ctggaaaata aacttctttt tcaatgatat
    11641 tatagggact acctttgaag tgtattttct ttgaatcagc tactacatgc acgattttcg
    11701 atccactata attctcagtc gttgtgttga aaacaaggac ttcattcgat tttagcttta
    11761 atgcatcagc catccgtttt acaataaggt tttcgattcc gtcagctatc tcattagctt
    11821 ctttgattcg atggaaaata tcttccgcaa cattatctga aacacagcca ccaatactat
    11881 catgagcctg gttttgcaaa atctttttcc ataaccgacg aagtaggcct ttactgatat
    11941 ggataccatt tctttctgca atcagcatga gcggctcaac tcgttttaga attttttgtt
    12001 ccaatttaaa attttctatt ttcatttttg ttcttactga gccaatagaa cgatgaacac
    12061 gagagtaaac cggctgacga agttcacctt tatattcagg taattctttt ttgtttctga
    12121 taacttctac ataagaagga taatctgaaa taatattgtg gaatttactg tatttattca
    12181 acttctctac gacttgatca aaatttaaaa tcattgactt ttgatctaca cccgaaggta
    12241 ctaaaatatc tttgtttttt ccttgcatat gaagcatttc aactgcttca tccaactttt
    12301 cttttgcaaa ttcaggaata tctttaattg cgtctaaggt catcattcca gtcatataac
    12361 caaatggaaa attcatggca taaacaaatt tatctcccaa tcctttccag ataaaataag
    12421 gagatggaac catcgtttca aagtttaatc ctcgccaaca catgaaatta tcgatttcta
    12481 cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa cgtgtcaggc aaatacccta
    12541 ccatcattgg atctccatat ttaaggatcg tatcactgat tccaatcatt aagtttctta
    12601 aaattgattc agcatccact aataaggcat ctgtttgtgc ataccacggt cccacgaata
    12661 gttgtcctgt tttcatcagt cgttggataa ccttaagctt ttgcggattg atctccaaat
    12721 actcatcgac gattgaagtt tgtccatcta aacaaaagtt tactttaggg ttcctttcca
    12781 attcatctag aacttcagta aagacctgat cagacaatac gatggcatct tcatttgtaa
    12841 aataccattc tctgtcccag tgcgtgtgtt gaattatatg tgcatttacc atatcagatt
    12901 ctcctacttt ccttcttcta ctgaatttac ttgttgttct tttttatact tgatggattt
    12961 tagtgcattg accattaacg ctgtaaccac tcccccaata acaattgata ctaaatataa
    13021 aggtatattc gtaacaaaag gaattgccat aaaggttgaa agcattgttt cttgagtaac
    13081 accaaaaaat ccatttagac cacatgctac tgcagtgccc accacgatgc ttggtattac
    13141 tcgtaaagga tctttcacgg caaacgggat cgtcccttca gtaatcccca caaggcccat
    13201 aatcatagct gtctttgcat tatctcgttc cgattcatca taaagttttt tgtttaaaat
    13261 tgttgctaaa aacattccaa atggcggaat agcaattcct aataacatgg ctgtactagg
    13321 tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca tatgcgattt tattgatagg
    13381 tcctcccata tcaaacgcca tcattcctcc taaaatagcc gacatcaaag cagcatttgc
    13441 tcctgacatg ccttctagcc aattagtcag tcctgttgtc attgcttgaa taggcgtagc
    13501 tagtacatac cataatatga ttccaacaac taatgtagta acaaaaggaa tcagcataat
    13561 tgattttaga ctaataactt gcatcggtac ttttactttt ttcaatgcat tagctaaata
    13621 gccacaagct aatccagcaa ccattcctcc aataaatccc ataccaagat tttgacacat
    13681 catcccagtg ataaaagctg gtgctagacc gggacggtca gcaattgaat atgcgatata
    13741 ggctgaaatc atcggtacga taaactgtaa tcccgtctga ccaatcattc gcaggactcc
    13801 ccagaatgta tccgtctggt cccaaacacc tgtgccacca aaaatgactg caatagaagt
    13861 tagtaaccct gctgctacga caagcgggat catataggaa accccacttt ggaaatgatt
    13921 tagaatttct cttccttgtt tcttgaattc cattagtaac tcccccacga tatatttttt
    13981 atttgttaat taatgcaatt gcttcatcaa tcgtctcttc accatctttt actacctctg
    14041 aggtccctac tgttaaaatc ggtttaccgt caaagcgacc tcgttctgaa acatttgtat
    14101 ctactgcaaa tatgacaaca tctgcctcgg aaacatccga aattgaaagt ttgttttcta
    14161 tccccatagc cccctgtgtt tctactttaa tatctaaccc tttcttcttc gcaaactttt
    14221 ctaaattagc ttttgccata taagtatggg caatccctgc gatacatgct gttactgcta
    14281 ctatttttgt catatgaaat tcctcctttt aaaatgatag agcttttacc aattcttttt
    14341 tatttctcga atcttttatt cttttaacaa actcgtcatc catcaatctt ccagataatt
    14401 ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc ttttattgct aacaaaaaga
    14461 cgagatcaac tggttgatca tctaatgcgc cccattcaac agaccgcttt aacttggcta
    14521 cagccactgt tgattcaatt actgctaggc ttttgccatg aggaatggcg atatttttcc
    14581 caattcctgt ggtcatatgg ttttctctgt caaatacgtc tttttgaaat tgtttgtggt
    14641 cagacacaaa cccattagtc tctaacaatt tcgataattt ttcgactaca ctttttttat
    14701 cagatgcatt taaatctatc actatattat ttatattgat tttttcactg atcatttata
    14761 agccttcttt caatatataa actaattttt caaacgacga actcgttttt aaatttgcta
    14821 ataactcatg attcgaagcg atttcattga atgtttcata aattttattc aattcaggtt
    14881 ttactttttc attaagtgca aaaaagaaaa cagaatgaac aagtgtccca tcccaatcaa
    14941 ttccttctgg attaacgtag acacagatta ctggcttttt tataaaatct acttcgatat
    15001 gcggcatagc aaaatcattc atcgctgttg aagatagttt ttctcttttg attgctccct
    15061 ctagtatccc ttctttcgcg taattttctt ttaccaattt tttggtaatt atatttaaaa
    15121 cgttcacttg attatccttt atttggtggg taaaaaagca aaatcgttta tctaataaat
    15181 ttagaaaagc atcttctgtt tgttcattta actgtagata ctgttttact tttctgacat
    15241 ctgcccctaa catcaaagga ctaacaacaa taacaggaaa atttgtattt tcaatcggaa
    15301 tcgtgcttat aacaagttca tcagtgactt ttctatcttg tagttcattg atgcttagta
    15361 catcttctat ctcaaccata tctgcaaaac cgttatttat tctttgctct aataattttg
    15421 atgttccata tccgctacta caaactaata tcactttcgt tttgtcaggt ttgtaacgat
    15481 ccaaaagtga ttgaatatgg agtgttacgt aagcgatttc atcttcatta aaatgtatac
    15541 agaatctctc ttcgatttcc tctaataaat ctacacttat cataaatgac cttttaaaag
    15601 aatatttgat tttgtccgta taagggttat agatatttac tcccaatttg agtcttttaa
    15661 ctgctgcatt gagatgtaat actagattat tgatcaattc ttcatcagga taaaattcat
    15721 ttaatttaat tcttactaac tcttgtagct caacacccat atctaaatag gaaagcttcg
    15781 tttgatctaa gtcgtgattc atagcatttt tttcaatcga agtaatgtga atgtcaacat
    15841 aagcttgttc atctattggc acaataattg aaaatgcttt ttcaatcatt ttgattaaaa
    15901 taagtgtatt ctttgagatt tcttcttcag aattattact acgtgataag aaattgcttg
    15961 tttcaatcct ttctatcgca atagcaaggt ggatagctaa agtttgaaat tcataatctg
    16021 taaatttcaa ttgtgtctta ttcatgtatc gattaagcaa ttcggctact tttttcaata
    16081 tgtccttatt taagaaatta ttcgcttcac caatgaaatt gatattcaag tctaagagat
    16141 ctgttttact gttagatgct gtaaagccac cccaataata gttcacaact tctgacatca
    16201 accttctttt acttttttca tcagctttga tgcgcacgcc atttcgatct gaaactaagg
    16261 acatcccatg cttgtttagt ctttttctaa catctttcat tgtgctttct accgtcgacc
    16321 tgctcacgta taattcgtca gcaagatttt gaatctttag agaatcatta gtattcaata
    16381 atttagcaaa cacataagtt agacggtcct ccggagagtc tgcgatattg attttatgac
    16441 tggataataa atttcgaagt gtttcttttt ttccaataag cgaaacgcct tttttaggtt
    16501 gaaccaacat ttgtacatct ggcatttttt ggattatttc tttcaaaatt aaaatatctt
    16561 tgctaattgt tttcgtagat acatataatt gttctgataa ttctttgtaa gttgtgtgct
    16621 cattttcaat caaaaagtat agtattttca tttgccgttc gctaaattcg atgcccatta
    16681 tcttcacctc ctgaatacag cttaagtgta caattattat ctctattttc atacagaaga
    16741 aagttcttga ttaatgtaga agtttctaat attcttgatt atttcaaatc aataaaaa
//
LOCUS       DACCXM010000055        16682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCXM010000055 DACCXM010000000
VERSION     DACCXM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.057"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..546
                     /locus_tag="IVY61_001567"
     CDS             <1..546
                     /locus_tag="IVY61_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP7970682.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="IVY61_001568"
     CDS             735..1403
                     /locus_tag="IVY61_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP7970683.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1833)
                     /locus_tag="IVY61_001569"
     CDS             complement(1603..1833)
                     /locus_tag="IVY61_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970684.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1960..3342)
                     /locus_tag="IVY61_001570"
     CDS             complement(1960..3342)
                     /locus_tag="IVY61_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7970685.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3498..4685)
                     /locus_tag="IVY61_001571"
     CDS             complement(3498..4685)
                     /locus_tag="IVY61_001571"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP7970686.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4692..5723)
                     /locus_tag="IVY61_001572"
     CDS             complement(4692..5723)
                     /locus_tag="IVY61_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7970687.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5769..7193)
                     /locus_tag="IVY61_001573"
     CDS             complement(5769..7193)
                     /locus_tag="IVY61_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7970688.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            7368..8273
                     /locus_tag="IVY61_001574"
     CDS             7368..8273
                     /locus_tag="IVY61_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7970689.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8364..9041
                     /locus_tag="IVY61_001575"
     CDS             8364..9041
                     /locus_tag="IVY61_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7970690.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9322..10218
                     /locus_tag="IVY61_001576"
     CDS             9322..10218
                     /locus_tag="IVY61_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7970691.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10273..11643)
                     /locus_tag="IVY61_001577"
     CDS             complement(10273..11643)
                     /locus_tag="IVY61_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7970692.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11881..12627
                     /locus_tag="IVY61_001578"
     CDS             11881..12627
                     /locus_tag="IVY61_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7970693.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12624..13463
                     /locus_tag="IVY61_001579"
     CDS             12624..13463
                     /locus_tag="IVY61_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7970694.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13464..14414
                     /locus_tag="IVY61_001580"
     CDS             13464..14414
                     /locus_tag="IVY61_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7970695.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14555..15307
                     /locus_tag="IVY61_001581"
     CDS             14555..15307
                     /locus_tag="IVY61_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970696.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15326..16477
                     /gene="queG"
                     /locus_tag="IVY61_001582"
     CDS             15326..16477
                     /gene="queG"
                     /locus_tag="IVY61_001582"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP7970697.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5429 a         3102 c         3341 g         4810 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt gataccgttg actagttctt ccatcgcaaa agcagtcccc
     1921 cattttaatc caaatggccg atctcgatac tcgttatagt caatgaattt ttttgctgtc
     1981 cgtctcatct tttccacctt ctaatccact cataccacct gcatgcccgc caacgagaga
     2041 actacgtgcg atggcgcgtc ctccttgcaa gagactatgc gcatggatga ttgattgaaa
     2101 gccgtatttt tttctaatcg tatcgacaac aaaatcaact ttcgcttcat ttatctgctt
     2161 ttcaggatct tcaaaaaggt taagctgtaa acctgtcgcg tatacgaggt cggaacagtt
     2221 cacgcctaca cgccgaatat cctggtagtg gtaatgctgg tggaaaatgg tcaataaagc
     2281 atctgcaaga tctctgctag catttgtcgc ttcgattttc atttgctggt gaaacccggt
     2341 cttgccattt ggatcaagat agcctaaaga gtatcctacc cataagctga ctacttgtgt
     2401 ttttgctgca gatcgtctca agcgagtgcc gacttggtcg gccatttctt tgataacgat
     2461 ctcgatttct tctcgtcttg tataatctct atctaggatt tgactatttc cgatactttt
     2521 tgattttggt gtatattctt gtcccaaaaa acttcgatcg atgccccaag catgggcata
     2581 aagctgagta ccgatgaccc ctagcttgct tttcaagtga taataatccg cgtgagctaa
     2641 atcataaaca gaatgtatac ccatttgttt cagacgtttt gctgttcgtt tgccgattcc
     2701 ccaaaagtca gtgatatctg agattttcca taacttttct ggtacatctt cataacgcca
     2761 ttcagcaaac atttgctttc cgtcttttga gccattgtct aaggctagtt tagcaagtag
     2821 cgggttatcg ccaattccta tagtcgtgta gattccggtc tgctcaaaca catctttatg
     2881 gatcagttta gccatgtcgt acgcatttgt tttaccaaag agtgccatgc tatttgttac
     2941 atcaaggaag ctttcatcga cggaatagac atgatggttc gcttcgtctg catatttttt
     3001 atagatagaa ttgatttctc tgttcttttc catataatag gccatccttg gggcaacaat
     3061 atataaatca cttggatagg ggaagggaag atctctggca cggctgatat tggtgatgcc
     3121 gtaagctttt tttgctgcag gactggaagc caagattaat ccgctacccc gtgtttctgt
     3181 cgaatcggac ggataggaca tgactactaa tttagttgta aggggatcaa gatccttttc
     3241 aacgcactct acggaggcgt aaaaagattt gcagtcaata cataaaatat ctcttgaagg
     3301 ttctttttca taaacaaata ccggattttg attcgcccac ataacaatca cctcttcaac
     3361 acaattagaa cacacgttct ctttccgtgt caataaaaaa cagcaatttt caaatacgag
     3421 caaaaggctc acatttggaa attgctgaga ggttattttt atctatttgt ttttagctga
     3481 gatcgttgat ttttttatca aacatgaaca gcttctggat cgatctttgt tgtcccattt
     3541 ccaatgtgtg caacataaaa ttccggagca tagccgacaa ctttcatgta ttcatcatag
     3601 acattgtttt tgaaggcagg tatattttct tctttgacaa gagcaatcgc acagccacca
     3661 aatccagctc ctgtcattcg tgcacctaag acaccttctt gtttttgagc agcatctact
     3721 agtgtatcta attcgatccc agttacttca taatcatagc gaagagagtg gtgagaatca
     3781 tttaacagtt gaccaaatgc ttctaagttc ccagcttcta attcagcttt tgccttcaaa
     3841 gtccgctgat tttcagaaac ggcatggcga gctctgcgta tcaatgtatc atcgccgatt
     3901 tggtcaatag ttgattcaaa ttcttcttca tctaagtctc ctaaagaaga gataggcaaa
     3961 gttttttgca aacgtgccaa ggcttcttcg cattcgctac ggcgttcatt gtattttgaa
     4021 tctgccaatt cccgccgctt attcgtattc atgatgacga ttgcataacc atctagtttc
     4081 actggaacca tttcgtattt cagcgtattt gtatctaata agattgcatg atctagctca
     4141 ccgaagccga tggcaaactg atccatgata cctgaattga caccgatgaa ttcattttct
     4201 acttttttac ctgtttggac tagttccaag cgatctgttt ttaaatcaaa cagatcttct
     4261 aatacaacac ctactaataa ttctaatgaa gcgctactgg aaagtcctgc accattaggg
     4321 attgttcctt ccactaataa ctcaaaccca tgatcgattg agtgtccagc ttcttttaat
     4381 ttaaggacca ttccttttac gtaatttgcc cagtttgctt ttttatcata ttctagctca
     4441 tctaaagtaa aactaatgac accgatatcg tcaaagtttg tggaataaac taatatttta
     4501 ttatcgtctc gaggtgctgc tacaccttgc gttccataag tgatggaagc ggggaagaca
     4561 tggcctccat tgtaatctgt gtgttcacca atcaaattga tgcgtcctgg tgagaagtat
     4621 tgtgtagttt ttttaggtcc gaatgtttcg gcaaattttg tgtttaagct tcttttgatt
     4681 tccatgatga gttattcctt tcttgttttg attttgaatt ctgttactgt ttcaaatact
     4741 gatccggctt ttaaatgaat cgaaccaaat gctgagaagc gctctgcacc tggagctgtt
     4801 tgagtctcaa atgtgatgcc accgtgatgt gccagttttc ggttgcgcat ttctggtgtt
     4861 tctgtgccga aatttgcagt gaaaataacc acactagaag catctgtagc aatatctact
     4921 tggattcttt tatcggggct agtcaagcga gcggcttctt tacccaagcc ggtttctttt
     4981 aagaaaaatg gatgatcgat tccgtcaaca agattttttt gatccaaatc actagcaaaa
     5041 acggacgcta attttttagg tatttgaaaa tcgaaagcac ttcctgcagc attttctttt
     5101 acccctatag gaatactatc tgttcgcaaa ggagcataag cttcgctatt gagccaaagc
     5161 tcatgttgat cgattgactg agaagcatct cccgtcaagt tgaagtaaac atgatttgtc
     5221 ggattgaata aagtatcttg gtcactggtg ccgcgagagg tcactcgcca gatattatct
     5281 tttgtcaaag tatagcggat ttccacatcc attgtacctg gaaaaccatg ttctccgtcg
     5341 ggacttgtag tagtaaaaat gacagaagct tcattttcgc cattcaaaat gacataagac
     5401 cattttttta attcaaatcc aggagcacca ccatgcagat tatgtccagt ttttggatct
     5461 gtggctaatt ggtaagtttt tccattgaga gaaaagcggc cattttcgat tctccctgcg
     5521 gtgcgaccga tagaagcacc gatatacgga tctttttcga tatattcttc agcggtgtca
     5581 aatcccaaaa ctaattctcg atcatttgat tttaaactga cgattctagc acctaaatca
     5641 gagatagaaa gtgctagctt gtttttgttc tccaaggtta tcaagtgata accttggcca
     5701 aaatcttttt ctgtaatctt cattttcaag atcctttctt aatgggaagg aactactgca
     5761 tcgtgaagtt aatcttccat tgtatcagct ggagccaatt tgttttccaa tttctcaacg
     5821 atttgtgcat gttttttctc tgtcaatgta atcttccaag caaaaataat agcggagaaa
     5881 atcatcaaag cagcaggagc atagaatgca taagctttga aagtcgttac gccatgagca
     5941 gtgatatcag cagcagtagc attaccagtc atcccagaag cgatagcgac aaaccctacg
     6001 atcccgttag acaaagcacc agcgatttta tctagtaaag ggcgaattga tagagtaaca
     6061 gcttcgttac ggataccatt tttccattga ccatattcaa cagagtctgt gatcgtcatc
     6121 aaagcggata agaaaatcaa ttgttgaggg aagtagaaca atactagccc gatgataacg
     6181 attggcaaac ttgaacctgc gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc
     6241 cctccaaggt aaacgtagcg acgagagatg acgcgtgtaa gcattgggaa tagtggaaca
     6301 gctactaaac caagtaaggc agcaactaga ccaacgatcc aaaattcagt tggttggcca
     6361 agtacatatt tgaagaagta gaataatacg gcagttgttg ctgcattggc aatcgcataa
     6421 gcgatataag caagggcaag ccacatcaac tgatcatttt gagcaactgc tttgaacaca
     6481 tctttgatcc cagctttttc attttgctga cggatcgcag tatcgacttc ttttgtgccc
     6541 aaggcagtga taatcgcagt accgcctgaa ataacagcag caatcacacc gaaccagaac
     6601 cagcctgaag cgccttcaat atgtttatga gtaacaagat aggtaaagaa agtaacgata
     6661 gggacaacga ctagagtagt accatttgca cctagagaag agccgaaacg agcaaacgta
     6721 gcaaattttt cacgttcctt tgtttccgta ctcaaagctg gaatcattga ccaaaaagcg
     6781 atatccttaa atgaataaaa actatccaat aagatgaaaa cgattgcaaa taatatgata
     6841 aataaagtcg tattatgagt agctaagcca aagaaattgg taaagattgc taccaagagt
     6901 acggaactaa caacgccgcc gataactaac catggtttga atttaccgta ttttgtacga
     6961 gtattatcga tgatgccacc aatcaaagga tcaaaaataa tttcaactaa gcggatacct
     7021 acaactaagc ttgtaactgt agcaatcatt tttgcattcg ttgccttatc tgttcctgcg
     7081 aacatctggc ttgtcacgaa taccataaaa taagtgctta atgtaacata gaatgtgtcg
     7141 tgtccaaaag cgcctaaagc gtaggacaca cgttggatca ttttgctttt cataatctgt
     7201 attcctctcc tgttcaatat ataatactaa gtaaaccggt tacaacgaat aacaaagatc
     7261 ttttaatgac ttaaacttaa caagaaatcg cttccttttt aatatatttc tgtgactttt
     7321 atttaacttt ttgttgcatt atactaaagg agtaaggagg gatagacgtg gagcaatcga
     7381 tttttttaaa aaaggaacat caacaagtag aaaaagaatt gtattttgat ttttgtggtt
     7441 tctctaaaac gatgccgttt cattcttttg gtcctgctat tcgcaatgat tacatcctgc
     7501 atgtcgtgat ggaaggcaaa ggatcttatc atgtgaaaaa tcaacaattc caattgaaaa
     7561 aaggtgatct gttcttgatt cgtccaggag actcgacgtt ttacttatca gatggagaag
     7621 atccgtggat gtattgctgg ctttcatttg gtgggagtgc tgcagaagaa atcattcggc
     7681 agtcgctttt cagagaagac caatacacga tggtctcttc tgacatacaa aaatatatcg
     7741 acattatttt agaatgcatg aaagatacgg aagaaacctt ggaaaatgaa ttacagttaa
     7801 cagcattaac ctaccgtttt ttatcagtcc ttttaaaaga tggcggaaag gtcagcggtg
     7861 gtgtacaaaa ggttcattcc tctttagctg ttgaaacagt caaatacatc gaggcacatt
     7921 atgctgaaaa attgtctgtt gaggaaatcg cccagcattt agcggtcaat cggagccatt
     7981 tgtcgcgagt attcaaaaat catatgggta ccagtatcaa ggaatatttg atcggggtac
     8041 ggatcaatcg aggggcgttt cttctttctc tgacggatga atccgtggaa agtattgcct
     8101 atcaagtagg gttcaatagt ctagtcgtat ttagccgaat gttcaaaaag agtaccggag
     8161 aaacggctac gaactatcgt aaacgtatga aaaacgaaaa ttttaagaat attgctttgg
     8221 ataccttgaa aaaacagttg gaaaaacaaa ccgcagtttc ttggacaacc tgattagtct
     8281 ggttaaaatg acaagcatcg gcaaaagaaa cttgtggcgg ttatgctaca ctgtaaagcg
     8341 aagactaaaa aggaggaaaa gggatgcgta cacttatcat cattagccat ccaacgatcg
     8401 aagaatcttt aaatcagcaa tttttcaaag aagcttctgc tggtttacca gctacttggc
     8461 atcatttgga atcaagttat ccagacggaa aaatagatat agagactgaa ctacagctct
     8521 tggcccagca tgatcggatc attttccagt ttccgtttta ctggtatagt tctcctgctc
     8581 atttaaaaat ttggcaagat aatgtcttgg aacgagcagc acatgtatta aaagggaaag
     8641 aactaggaat cgtattgaca acaggtgtgg cggaaaaaga ataccaagct ggaggaaaag
     8701 aagaatatac gatttccgaa tttttgaggc cgtatcagcg aatcgcaaat aaattccata
     8761 tgacctatct tccgccattc gttttagcac aatttatgta tttatcccag gaaaaacgtt
     8821 gggagaagct aattgcttat cagcagtatc tctcattgga aggcaaacca tcgctgactc
     8881 agcggattga ctggtttatt caaagagtac aggaaaatca gaaaatgcag gaagaagaat
     8941 ctgaaaaaca agcatatatc gtagaagcac tgactgaggc aaaagagcag atcgaagact
     9001 tgacctttac actgcaagaa atgaaaggaa cttcattatg acaaatgatt ggctgatcca
     9061 acaattagat gaattaagcg aaaaatcaga atatcaagac cgtgcgctct tatatgaaat
     9121 aaagaaactg atggctgaga tagacgaacg aacaatgcag ctgcaaggag aaatcgacgg
     9181 acaatcttgg aatcatctca actggtgaaa ttttcttata aataacaaag ataaaaatgt
     9241 gcacagcgac aattatttgc tacaataaga atatgaagcg tttgataacg ctattataca
     9301 taaaaattgg tggtgaaaaa aatgtatgat gaaaatgagt ggggagaaaa tgttcggatg
     9361 acccgaagaa gacagcaaaa acaggcagct cgtaagcgga tgatcatgat tggggcagca
     9421 gttgccgtgt ttttgatcat agcaggagga gttgcgggtc tatattttca tggaaaaaat
     9481 tcgtcagaag cgaaaagtac gaccataacg gaaacaacga caaagaaaac agagacgaaa
     9541 caaacaagtg agtcatcaac taaaacaaaa gagacacaaa cttctgaaga aaaaacagca
     9601 aatggtttgc cagagcaact tcaagcaatc gtaaatgatt attcgaaaaa gatcgaagaa
     9661 aagacgcctg cattgattga agaataccag acagaaattc agggaaatca agagggaata
     9721 gctggtttat cagcagttgc taaccaaaaa gcacgtgagc ttcaagcaat ctccgacgaa
     9781 gggatcagaa aattacgtgc agcgtatcag gcagcagaaa ataaagacgg tgtagactta
     9841 gatactttga tcaatcaatt atcggctaat tatacgaatc atgtcgctaa gatttccgat
     9901 atttatttgc agactagcgc ctctcttcaa gcagagtcaa caagcacaca agacacaaca
     9961 tcttctgatt ctgagacagc agagtcggta gaagcttctg aaatgcaagc tcgaacaaca
    10021 caagacacaa cagattcctc tgaaactaat accaacagcg cgacttcaca ggctgcgaca
    10081 accgtagttt tggaaggaga aggtccaaat caaatagccg aacgaactgg cgtaccagta
    10141 gaaacgatct tgtcgctgaa tgggatgagc atggagaatt atttcctgac tcccggtgag
    10201 acgctaaaat tgaattgata aaagcaataa aaaagaacga agcttccact aaggtgaagc
    10261 ttcgttcttt ttttattttc cggttctttt ttcgcgtaca aagtaatgga tcgtcatgta
    10321 aagcaagaaa acgagtccaa taatcaatga gattctcgtt tcaggattga taaacatgaa
    10381 aatcaatacg atagctaaaa aagcaagtgt cacataattc gtataaggag atagtggcat
    10441 tttaaaagga tgatcatgca ttttatctgc gtgcttcttt ttgaagcgaa tctcgctgat
    10501 caaaatcact acccaaggaa ccataccagg caaaatactt gagctgtata cgaaaacgaa
    10561 aatattttct gactggttga tgaacattgg tagaagataa tttaagatca gaccaattaa
    10621 gattccacct gagattgcta cgacaggata aaaaggcgca ccgtgacgag atagtttcag
    10681 aaatttttta gacatctttc catttatggc aagtgtatag accatacgac ttgcgctgaa
    10741 aatccctgaa ttgcaacctg ataatgccgc tgtaatcacg acaaaattaa tcagtcctgc
    10801 agcaaatgta atgccgactt tagaaaaagt ttgtacaaaa ggtgaaccaa tctcactcaa
    10861 ttgattccac ggatagatac tcacaatgat aaaaatagca ccaatataaa aaatcaagat
    10921 tcgaccgata gtggactgga cagcttcaat gatcgtcttt ttaggattct ctgcttcacc
    10981 agcagtcatt ccgatcagtt cgattccttg ataggaagca acaacgatcg ataatgcaaa
    11041 gaaaaatcct ttgacgcctc cagtgaagaa tccaccattt ttccaaagat ttgaaatccc
    11101 tactgcctga ccatgattac caaatccaaa taagattacc ccaagacctg caatgatcat
    11161 caaaatgatc gttatgactt tgatgatcga aaaccaaaat tctaattcgc caaacgcctt
    11221 gacagaaata agatttgcca gcataagaaa caagacgaca atgattccgg gaataattgc
    11281 cggcaaatca ggccaccaat aattcatata agatcctaca gcaatcactt cacttattcc
    11341 tacgacaata tattgaaaaa tattactcca agcggtcaag tagccagcca caggatggat
    11401 gtactcgctc gcgtaatttg caaaagaacc agtcgaagga tcaacgtaaa gcatttcgcc
    11461 taaagcacgc ataatcaaat acaaaaagaa tcctgcaatc gcataggctc ccatcactga
    11521 gggccctgtc caacgtatgg ttgaggaggc acccataaac agaccgacac cgatcgttcc
    11581 acctagtgcg atcatttgga tatgacgggc actaagactt cgagttaact tttcttcttc
    11641 catacaaaaa ctcctttcgt tgttgagtca acaagaaaaa ataatcaaac gtttcaacac
    11701 atgtttttca ttattaggtt agccttattg aactcttgat gtgattctac agaaaatttt
    11761 cataaaatgc gaggaaaatt cgaaaaaaac tttttcatca ttctacttga aaatgctttc
    11821 cattttagtt aaactatatt taaataaaaa gttaggtaaa cctaaaaagg agtgtaagat
    11881 ttggaacgta cggccattgt aattcaagac ctttcagccg cttatcaagg gaaaactgtt
    11941 ttgaaaacta tcaacttgac gataaatcca caaaaaatta ccggtattat cggaccaaac
    12001 ggtgcaggaa aatcaacatt tatgaaatct ttgctagagt tgataccagc tactactggt
    12061 aaagtcacct tttctggaca gtcggtaaac tcagtccgta aaaaaatagc ctacgtggaa
    12121 cagcgaagtg aattggatct ttcctttcca attgatgtat taggcgttgt acttttagga
    12181 acatatccat ctttacgaat tggacaaaga cctgggaaaa aagaaaaaga acgtgcaaga
    12241 caagctttga aaaaagtagg gatggaagaa tatgccaaaa gacagatcag cgaactatcg
    12301 ggtggacagc ttcagagagt ttttattgca agagctctag cccaaggagc agaatggatc
    12361 tttttagatg aaccattcgt agggattgat gcgttaagtg aacgaaagat ctttgacatc
    12421 ttgcaggaat tgaagaattc aggaaaaacg attttgatcg tccatcatga tcttcataaa
    12481 gtagaagaat atttcgatga ggttattctt ttaaataaac agctgatcgc ttccggtcca
    12541 gtacaagaag tctttacatc gaagaacctt caattggcct atggtgagat catcagacat
    12601 ttagtaaaag gaggagagaa aaaatgatcc aatcctttat tgatggatta atgaattatc
    12661 aattcctgca atacgctttg gtcacttcca tgcttgtagg acttgcttct ggagtcatcg
    12721 gttcttttat tattttacga gggatgtctt tgatgggaga cgctatctct cacgcagttc
    12781 ttccaggtgt agccatttct tatatgtttg gttttagtta catctttgga gccactgctt
    12841 ttgggatgct aacagcagca gtgattggtt tcgtgactca gcggagccga ttgaaaaatg
    12901 atacagcgat tggaattgtc tttagctcct tttttgcatt agggattatc ttgatttcat
    12961 atgcccagag tgcgacagat ttgtatcata ttttatttgg gaatgtctta gctgttaggg
    13021 aaagcgattt gttgctgaca gcactcgtta gtggaatcgt cttgatattt gtctttttct
    13081 tttataaaga attgaagatc acatcattcg atccgacaat ggcaaaagct tatgggctga
    13141 acacatcgct gatccattat cttttgatgt tctttttgac attagtggct gtagtcagtt
    13201 tacagacagt aggaacaatc ttggtgattg ccatgttgat cacaccagcc gccacggctt
    13261 acttgctaac gaaccattta ctgaaaatga tcattacagc tgcaggaatc ggtatgctaa
    13321 gtgcagttgt cggtgtgttt ttcagttata gttacaattg gccatcagga gctacgatcg
    13381 tgttagcatg taccgcattt tttatccttg cttttttatt ttctccaaca aaaggaattt
    13441 tatttcagaa aggaagcaga tagatgaaaa caaaaaaatc actcttttta atcttggcag
    13501 ttagtttctt ggtattagcc ggttgcggaa agcaggcttc tgaccaagcg gatgaaggaa
    13561 gtaaagaaaa gttgtcagtc gtggctacca attcgatctt ggcggacatg gcaaaagagg
    13621 tcggaacaga tcaaatagat atccacagta tcgtaccagt cggaacagat ccgcatgaat
    13681 atgaagtact accagaagac atcaaaaagg caagcgatgc agatgttatt ttatacaacg
    13741 gtttgaatct tgaaacaggt aacagctggt tcgataactt gatggaaacg gctaaaaaag
    13801 aagaaggaaa agattatttt gcagttagca aaaatgtaga acctctttat ttaactagcg
    13861 gtgaagaaca tacaaaagca gatccccatg cttggctaga cctatctaac ggaataaaat
    13921 atgtggagga aatcgcacgt atattctctg aaaaagatgc agaaaatgcg acactctata
    13981 aaaaaaatgc agaagcatat gtggaaaaac taaaagaatt agatacacaa gcgaaggaaa
    14041 gttttgcttc tatcgaagag aacaaaaaat tattagtaac aagtgaaggg gcattcaagt
    14101 atttttcacg agcatatgat ttgccagcag cttatatatg ggagatcaat acagaaagtc
    14161 aaggtacgcc tgatcaaatg aaagcgatca ttgatcagat aagagcaaaa gaagtaccgg
    14221 ttttattcgt ggaaaccagt gtagactcaa gaagcatgga acgggtagca aaagaaacgg
    14281 gtttgaaaat ctatgataaa ctgttcactg attccatagc aaaagaagga gaacaaggag
    14341 attcttacta tcagatgatg aaatggaata ttgaaacgat ccatgaaggt ttgtcacaaa
    14401 caaaagaaag ctgaaaagtg caaggtctag cttgaggagc agaccttgca ttttttataa
    14461 gagatatgaa aaaaaattat gctgtttttc gtcgtacaga tacctttttt gaagaaattc
    14521 cgttatagtt agagtaagga aggaaggtgt ggctatgaat tttacacgta aagataaata
    14581 tacgattaaa gaagaagtct ttcgattgat cagccagcag gaaaataccg acaaggatca
    14641 agaccttcaa tttcttgaag cagaattagc taaattattc atagcaaatc aaaatgagac
    14701 aacagaacca gatgaagagc tgtacatagg caaatggttg tttgatgaat ttcagccttt
    14761 agatgaaaat accggtatcg gttattttgt gttcctttct ccagtcggct tcacacctgg
    14821 cgtattagtt aaacaaaacg acgaaataac cgtcaaagca tggcctgcat tattttcagt
    14881 catcaaagca gaaggagtaa tcaaaaagat ccagtcggcc tattataaaa gtcaatttat
    14941 tgccaacagc aaattaatga atatgtttta taatcgtcta gtagaaattg ctaggcctgg
    15001 gaaaatcccg caaacattca cgtacacaca gcaaaaaaat ctctatgaag aagtattgta
    15061 ttcccgcgat ctgtctgagt tttcgccatg ggaagaggta aggctccatg aactgttaag
    15121 tacaaaacca gtttcattga atttttttaa tgcattagat aacaaaagaa aattaatttc
    15181 aacaaattat tgggaagtat ttgctgcaag aaaaaaatgg atggaagaaa agaagattgc
    15241 cgatcagcag atccagtttg tccagctgaa aaaagaaaaa ctatattatg ggttatacag
    15301 ccgttaacag caagcgagga agaccatgac attaaaagaa gaaatcatac aagaaagcaa
    15361 acgattagga attgacaaaa tcgggttcac cacggcggaa ccttttgatt cattaaaaga
    15421 atcgttggaa gaacaaaaag cagctggcca tacttctggc tttgaacatc caaatatcga
    15481 tgagcgtctt tatccagaaa tgacatttga tcagccgaaa tcgatcattt ccattgcact
    15541 tgcgtatcct actaggatcc atgaagaagt gcctagggat gaaaaacgcg ggcagtttgc
    15601 tcgtgcttct tgggggatcg attaccatga tatactcaga gaccgtttga accgattgat
    15661 cgaattcatc caaacaagag cagaaaaatg gcaaaaagaa gaggaatggc gtctagctcc
    15721 gcaagtagat actggagaac tgatcgatgt cgctgtagca cagcgagctg gtttaggatt
    15781 tattggacga aacggattgt tgatcactga agagttcggt tcatttgtct atcttggtga
    15841 gatcgttact aatattgtat ttgagcctga taaaccagga gaatttggtt gcggtgactg
    15901 tatgcgctgc gtgactgctt gtccgacaaa agcattgctt ggtgacggaa gaatgaatgc
    15961 ccagcgatgt ttgtcttatc aaactcagac aaaagggatg atgccggaag aatatcgtaa
    16021 aaaaatgcga aatgtcattt atggatgtga tatctgccag ttagtctgcc cttacaatca
    16081 aggaaaagat tttcattttc atgaagagat ggaaccaaaa gtagacgaaa tatatccaaa
    16141 attaaagcca atgctttcga tgtctaataa agacttcaaa cagcaatttg ggcatttagc
    16201 tggttcatgg cgtgggaaga aacctttgca gagaaacgca ttgattgctt tagctaatct
    16261 aggtgatcga acggcattgc ctgagatttc agaatgttta acagatgtcc gccctgtcat
    16321 cagaggaaca gcagcttggg ctattggacg tctaggaaca aaagaaccgg aaaaatggct
    16381 tgatgtatta gatgaaatat tagctaaaga aacagaagaa gtcgttataa acgaattgga
    16441 acatgcccag aaattattgc ggagaaagtt aaaataaaaa aatagttaac attattcaaa
    16501 gtttttgtcc agtaattgtt gtaacaatta ctggtttttt tgtatttttt acattttttt
    16561 aagagaattt gataaaaatc aaaaattttt ttgctttttt ttatggatta ttgcagtgaa
    16621 tgttacagaa tctgcgtttt tcattcaaaa ataaaaaaac atagaaaaac gattactttg
    16681 ct
//
LOCUS       DACCXM010000056        16635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCXM010000056 DACCXM010000000
VERSION     DACCXM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.058"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..2131)
                     /locus_tag="IVY61_001583"
     CDS             complement(<1..2131)
                     /locus_tag="IVY61_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970698.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWM"
     gene            complement(2128..2832)
                     /locus_tag="IVY61_001584"
     CDS             complement(2128..2832)
                     /locus_tag="IVY61_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7970699.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2844..5153)
                     /locus_tag="IVY61_001585"
     CDS             complement(2844..5153)
                     /locus_tag="IVY61_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7970700.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5348..5812)
                     /locus_tag="IVY61_001586"
     CDS             complement(5348..5812)
                     /locus_tag="IVY61_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970701.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5812..6438)
                     /locus_tag="IVY61_001587"
     CDS             complement(5812..6438)
                     /locus_tag="IVY61_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7970702.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6445..6810)
                     /locus_tag="IVY61_001588"
     CDS             complement(6445..6810)
                     /locus_tag="IVY61_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP7970703.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6800..7138)
                     /locus_tag="IVY61_001589"
     CDS             complement(6800..7138)
                     /locus_tag="IVY61_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970704.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(7128..7454)
                     /locus_tag="IVY61_001590"
     CDS             complement(7128..7454)
                     /locus_tag="IVY61_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7970705.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7435..7713)
                     /locus_tag="IVY61_001591"
     CDS             complement(7435..7713)
                     /locus_tag="IVY61_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP7970706.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7715..9079)
                     /locus_tag="IVY61_001592"
     CDS             complement(7715..9079)
                     /locus_tag="IVY61_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7970707.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(9092..9667)
                     /locus_tag="IVY61_001593"
     CDS             complement(9092..9667)
                     /locus_tag="IVY61_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP7970708.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9633..10820)
                     /locus_tag="IVY61_001594"
     CDS             complement(9633..10820)
                     /locus_tag="IVY61_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7970709.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10884..12611)
                     /locus_tag="IVY61_001595"
     CDS             complement(10884..12611)
                     /locus_tag="IVY61_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7970710.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12608..13060)
                     /locus_tag="IVY61_001596"
     CDS             complement(12608..13060)
                     /locus_tag="IVY61_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP7970711.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(13172..13552)
                     /locus_tag="IVY61_001597"
     CDS             complement(13172..13552)
                     /locus_tag="IVY61_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7970712.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13549..13935)
                     /locus_tag="IVY61_001598"
     CDS             complement(13549..13935)
                     /locus_tag="IVY61_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970713.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13937..14215)
                     /locus_tag="IVY61_001599"
     CDS             complement(13937..14215)
                     /locus_tag="IVY61_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970714.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            14263..14991
                     /locus_tag="IVY61_001600"
     CDS             14263..14991
                     /locus_tag="IVY61_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAP7970715.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(15137..15613)
                     /locus_tag="IVY61_001601"
     CDS             complement(15137..15613)
                     /locus_tag="IVY61_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAP7970716.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15573..15920)
                     /locus_tag="IVY61_001602"
     CDS             complement(15573..15920)
                     /locus_tag="IVY61_001602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970717.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15907..16092)
                     /locus_tag="IVY61_001603"
     CDS             complement(15907..16092)
                     /locus_tag="IVY61_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970718.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(16093..16365)
                     /locus_tag="IVY61_001604"
     CDS             complement(16093..16365)
                     /locus_tag="IVY61_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAP7970719.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16362..16565)
                     /locus_tag="IVY61_001605"
     CDS             complement(16362..16565)
                     /locus_tag="IVY61_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970720.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
BASE COUNT         4664 a         3523 c         2583 g         5865 t
ORIGIN      
        1 ccatccattg tccatttgta aatgtccaaa taagcaatcg aacttgtgca tctaagctcg
       61 ttattggttc tttaatacct gcagcattaa ataagttttg aacataaact tttccatccc
      121 atgttgctgg accaacaaga ggatatgaag aaccatccca ctgaactaat ccgtatgctg
      181 gtccacctat ttgaacagta tcaggatcaa aagttcctcc tgtttcttgt tgaatgttcc
      241 ccaatattcc acatgcagat tgtttcgtaa atccgttatt acacaaaata tcgtatattc
      301 tccaagctct cttttctgca tctgttttta attcatcagg atacccacca ggtgtttctc
      361 ctccgccaga tggaccgcca ttttgtcctg gtatcacttc tttacctcta acagtaagtt
      421 ttccctgaat atctaaatca ccgaaataat atgcttttcc gtttcctaat aacacaaatc
      481 ctttttctgc agttggagaa attaaaatgt attttccatc gccatttgta cgtattacca
      541 aagaattttc ctcaagaggt gtaggtgtta cagcatttgg aaatggattt cctgctgaat
      601 ctgttgttcc gatcgttcca atggattctt ttctattcca gaattccata ccttttttgg
      661 ttagctccat gatttttttg ttttcattcc aaatttgaag cgtgcctttt acaagtttta
      721 acacatcacc ataagcatta aaggaagttt cgaatacttc tgcatttatt gttcctactt
      781 tgatgaagtc agcaacaatt tctccttttg atgtgatagc aataccgaac gggccattta
      841 ctccgttgtc tgaataacct aagccattta aattccaacg ccacactcgc tttgcattcg
      901 ccacatttgg agtatccatg ataagaatct ctgatggagc tttttctgga cgaaaaacga
      961 catgcccacc agaattacca gtgatccatg ccgtcgcatt caacacattt tgaactaacg
     1021 tttctgttcg attgtcgatt ttctttttca attcttgtgc ttgagtattt actgccgaag
     1081 tgtacagtga taaatcatta cctaaaacaa tatccttata tttcccaagc gttgggaaat
     1141 aagtgtattc gaccatacgc tcttttatct cgatatctag ctctttcgcc ctaacatgag
     1201 cgacatcacc aaagtgtaaa gacgctagct cttgatacat gtcaccgtat tcgagtgtgt
     1261 gttccaacgc caccatactg actgtatgag tagcttttgg ctcatggata cgatcattgt
     1321 caaataaggt tttgccccat ttgatcaatt catcaactgt tttacaatct ccgttttcac
     1381 gtttggcaat acgtcgattc tcgttggtta ctccatcaat ttctaaataa ccatattcga
     1441 taggttcttt gtcttcatca taatcgttat caggaacacc gccaaccaaa tacaaactat
     1501 tgacaattga ttcttcatca atttcttctt cgattgcttc taaattgatg ccaaaatcaa
     1561 tccgaaaacc attatctgat ccaatctgtt ttaccagttt caaatcatag ttatccatat
     1621 caagttccgc gctagtcacg ccagtcaaat tttgattacc gttatttgaa ccaatgattg
     1681 cttcaactgg cgcgacttgt cttgctgtaa attggtgcgt tgtccctaca ttcgaaagat
     1741 agttaaattt ttgatcaaat gccaaactgt ttttaagatt ggtcatgatc tgagacccat
     1801 tgccgttatc cgtaaacgac ttgacaataa agttcttatt ggccataaaa ccaatatgcc
     1861 gagctgttac agaaacggag gtcaaattct ttttgacatt ataaatttca aaatattgcc
     1921 atgatccatc tggtaccttc gcttttataa aatttccttt ttttaaataa gagcgatact
     1981 gtccgcttct tgaatagtta ccataaaaac gatattggcc attcagcaca cgattgattt
     2041 ctggcaaatc ttcccaatca actaacgacg ctccgttcac gctcaaatca tctggcattt
     2101 ttttataagc ataaataaat tcttgcgtca cagatacaca ctcctattcc aaaaccgcac
     2161 ttctttaaat tttccggata tttttacctg attccattca ggctgcaata ctggccaatc
     2221 accacgcgta aataaattca aaccttcttg tattgctttt ccaagctgag tatcaaccac
     2281 gattgttgca gctaacgtat taagtattgt taggctttta tcaccgacag cgatcgttat
     2341 atctcctccg ttcgattcta tttccagata gggatgtgca atttcgtcac catgatcaaa
     2401 aatatccata acactagaat gaaaaatttt aggtgcttca ccgatttttc ttttgagtgg
     2461 ctgacaacga aaagtaacat cgaatgtata aaagaagccc cactcgtttt cgaaagggac
     2521 ttctttatcc atgctgcaga tagcctctag atacttgtct ggatcattat gtgtgattaa
     2581 ttggctttta ccagtgagcc atcgtttgac ttctctcagt ttcgaatgtg gaatagtgat
     2641 cccttctatt tccaaatcaa aaggttcata gtcatcaaac gtctccgtca attctccgct
     2701 tcggccctga atcgtgtaag tttcgtatcg cttgttcggc ataatatctg gaagctctgc
     2761 ctcaatgatg caattcatat caattactgc attccgattt ttccaaataa aatttggttc
     2821 atctggattc ataaattgtc gactcaaata ggaacacctc ccaaatcacg aatagcctgt
     2881 ttgtttgctt tagcaaattt acgattcata cgatctaact cagatggatt gtttgcatcg
     2941 actttgccaa tgtgtatatg ctgttcaatg ctacctccag aaactcttcc actgatccct
     3001 ttgcgttttt cttcgtctga taatggagta actgtggttt taccattctt ggcagtcagt
     3061 aactcaggtc cagcttcacc aacaatcgct tgtccattga ttagatgacc gccttgtgct
     3121 aaataaggca gttttgaaat agaaaatgat tttccaccta caccaggaac ccattttggt
     3181 atctttatat tattcaatcc accaataaat ccattgatca atccaatcat ggcattaata
     3241 ggagctttcc ctacagcaac aataccatcg aaaatacctc caaaaatatc aactagacct
     3301 tgccatgctt ttgaccaatt ccctgtaaat actccagtga taaaatctag aaaaccactg
     3361 aaaattcgtt ttccagcatt ataaaaatta ttgaaatttg ctataacacc atcgaaaaat
     3421 ccaccaaaat aaccacttaa aaaattaaat atttcaacag ctacatctga aacacctttg
     3481 aagaaagaat tcactccatc atgaaaccat ttaacgttat tatatgccca aattagtcca
     3541 gtaactaatg cgccaatagc tataactgct aatgcaaacc agcctcccga cattccaaat
     3601 aaaccagcaa gtccttgcca aactcctata aaacttttaa cttccccaac gatttttgta
     3661 acagatccca taagtgaacc aagaacgatt agtactggtc caactgctgc cgctatacca
     3721 gcaattgtta tgatccaatt ctttgtatct tgatctagag acccccacca tttagagaat
     3781 tcctgtaata aggaagttgc tttttcaaaa aaaggcaaaa ggcttacttg gactgcttca
     3841 cctacgtcag ccattgctaa ttttgcattg ttcatagctt gatcggcttg atcaattggg
     3901 tcgagagtag catcgaatgt gtctcctaca gctcccccac tttctccagc agtttttgct
     3961 aaatcttcca ggttcaaagt acctctacga attgcatcgg ccattctagg accgccttta
     4021 gtcccgaata cttctgctgc tgcattgatg gcttctgttt cagaacctgc attttttacc
     4081 ttgtcttgta gttcttctag tccttgactt aaagattttc cgtctttcgc ataagccact
     4141 gttgctttag ataaactact taaagctgcg cttgaatcaa caccagattg ctcaaattgt
     4201 ccaagtaaag tgaccccttc accaaagctc aaccctaatt gtttaatttg tggtgcgcca
     4261 tcaattgctt tttgcatcaa gtcgtctaca gattgaccag tattctgcga tgttttcgtt
     4321 gttacatcaa ggacagaatt caaatcatca tattctagtc cataagcatc aatagcttgt
     4381 cgagcagata ttgctgattg cgaaacatct gtatcattga tttcagcgta ctgtaggaga
     4441 taattggttg aatcttccag ttttttatcc atgaatccaa attgtgtatt tacctcacca
     4501 atagcttccc caaccgtttg taaaggtaaa tgagtatttg aaccaacgtt tttgaaagac
     4561 tgtgaaagtc tatcagcttg atcacctgtt gctccggttt ttgtaatgat ggtatcaagt
     4621 gcctcgtcaa cttcaccaaa tgctgcaagt cctgctgctc ctgccgctac aataggcgcg
     4681 gttactccaa cagacatttt ttcaccaacg ccttttactt tctcacctgt ttcttcgatt
     4741 ttctggagtt tttttgctgt atcaacagaa acgtcacctt gttctttaag tgcatcgttc
     4801 gtctgttcta atgctgtacg aagtttattt tctccggttt cagaattgag taattgttta
     4861 taaagttttt ctgattcagc agaatattca ccggtttctt tgacagattt ttcatattcc
     4921 tctctcagca tttgagatcg ttgttcagcc aatcctaatt gtttttctaa tttttgttta
     4981 gccgctcgca atttttctgt ttgagttgca tccttgtcca tcgcagacac ttggtttttg
     5041 tactcggcag cggctaagtt catttctttg ttgatttctt tgattgtttt cgaataactg
     5101 acttcgccat tcgttttaaa attaagaacg acatcagatt ctttaccagc catttatctt
     5161 agcgctcctt tctaccacca aggcgattta tccatagtta cagattgagg tggttcaaaa
     5221 tcggtgtttt gttgtaacca ctgtaaataa gatttgagcc acaagttagg tgtagacttt
     5281 aaaaagaaac tctcactcca attcaataga gtgagagcaa cgtaaatata aaaactccaa
     5341 ggagttccta tctcttccga ttctttttgt ttttctttct ttttgcttgc gctttttgaa
     5401 agtcttgtgg cttcttggat ttttttaggt cttccacctg aaatgtctgg ctagcaaaaa
     5461 tttccataca ggtactataa gcagacaaca cctctccact cattcccaaa aatttgaaaa
     5521 tagtttctgg atcttcctct aaaccaccag tacgcagcat ggcataaatc aaggcacgca
     5581 tgatttttag atcactaggc gataagttag cagaagaaat ttgtccttct tttttagata
     5641 gcatggcgtt catatcagat tcaaattgcg aataatctcc accatacaca tctgctataa
     5701 attccatcgt ttgcatagta aatgaaatag ggaattctgc gccttgaata attacaacag
     5761 cagaattttt taagtcttca acgtgaattc cataatcaga taaccgagcc attaacccgc
     5821 acctccaact ttagccaatg ttttccactg ttcttcatcg tatacaggtt gagcaatgaa
     5881 tttattaaat aattctagag aagctccgtc tctatttgaa tcaaaacttg catacatcac
     5941 attattgtat ttcagtccga tggaaaccag atttgcagtt acatcgtcaa tttttgtttc
     6001 ttcctctgca gtggcatact cttcgtcaat cacattggat agttgtgtat taggatacca
     6061 aactgctttt ttaccgccat tttcaatatt tccaataaat ccaaaggcga agtacggaaa
     6121 ttctcgtgcg gtatttttgc taaacgttac tccgctttta gcaactaatc ctttgatttc
     6181 atccatcact tctatcggaa tacccacgtg atccaacgca agttcatgtt ttgtttctcg
     6241 cgacacacga cgaaacattt tgctggatgc ccatttttct aatgctgttc cattcccttt
     6301 gacaccgatt tttgtggcaa ttggcaatcg taaaacttca ctataagtcg gtgcagttcc
     6361 aacagaatct ggcgttgcca tcatcgcaat taaaatgtcg tccaatcctt caaaataaaa
     6421 agtatcttgt tttcccaatt aaaatcaccc ttcccataaa tcaagtattt ttctagtcat
     6481 aatttcttca attttttctt tgttttgttc atacgtacca cttgcaaaat gttgggcacg
     6541 ttgatttgtt gtgccatttt cggcaaagcg ccagtaaaag gccgtctctt caaattgaac
     6601 tttcacttga tcgttttcaa ctactacttt tatttgatcg cgcatatgtt tcttttttaa
     6661 gagcgactta ggaatattag gaagcaactg ctttagataa aagttagctg cttcttctaa
     6721 agattccaac gataattttt tcggatcaac ttttgaaagg tttcctaaat aatccgatat
     6781 ttcagaaaaa ccattcttat tacttggcat tttccacaca cctcacatat gtgtaatagt
     6841 tggttacggt atcgtcgttc tcatcacctt gtatacctgt aaaatctgaa tatggaatgc
     6901 cagcattttg cagcgcttgt tcaataacaa ctaaatcctg ttctgttcca agtgtaaaaa
     6961 aagagacttg gtaataaggt aattttttat agactttacc ggatgccatt tttttgctgt
     7021 tactcacatt tgagtacaca atgtatggat acatcgtccc taatctggct ttgtctctga
     7081 acactgctaa ctttgttgat ttcagcgctg ttttcaattc atcaaagcta atcgacataa
     7141 gctaaactca actccatttc tcttgcatcg ggattcgtat aaatgcgagt aatgttatac
     7201 gttacagaat caattttgag cgcacttaat ttctctgtga tggatttatc ccatctgact
     7261 ttaattcgtc tgacaacgtc tgtcttggct tgctgtgata aatatttttc ttgagaagtc
     7321 acaccgagtt cttcatagaa tattagacgc ttaaattcgt aaattgtagt tggacgatcg
     7381 tttccgtctg ttcctgtttt gatgtctagc aattcggctt tccatctgag attattagtc
     7441 tgtctcttcg gcattttgaa tcgctccttg cacgataaat ggcgtcatgg cattcatagc
     7501 tttgtcgagt tcatcctctg aaactctata ttcataggca atgccggcaa ccatcaaaat
     7561 aagatattct tgttggccac cagttgctgt tttgacataa tcttttgcca tatttaaata
     7621 aaaagagagc aaagaatcat ccatgccctc ttcaaaatga atatgtgatt tgaatttttc
     7681 ctctaaagac aattctttag tttgctcttc catcttaacc accaactggt tttgtaattt
     7741 cgtagcgata tactgccggt tcaaatggag aataaaccaa ttgaccatct agcaagttgt
     7801 aaatttggaa tccaatttga tttttaccag agaatttttc aacaagtttt tgaatttcca
     7861 aggcaccaat aacttcttga attttaaatg cagaaaaatc gccaaaatat aaaactggcg
     7921 tgtctggttc acccttttta tctgctgcat ctgtccaatc cacaggatag ccaactaatt
     7981 ggtaaccaat tccaccttct gcttgtgtga atggccgcaa caaaggaaat ccatcatctg
     8041 ttttcatttt ttcaatggca gtcaaagcag ctcgattaat aataaagcgt ccctttttca
     8101 tcacttctgt cactggtgta tttttaaatt cgattaatgc atcatataat ttttgcccag
     8161 cacctgaagc agttagatct aaaggttttt taaatgctac agcctttttg gctaatgcac
     8221 caggattttc atttccagcg tcatcaccat tgaacatata attgatttct ttacgcacat
     8281 aagctttttt caattcttcc acaacaatat cttcaactgg aacaccagac atttttaata
     8341 attttttagt tactgttgcc aaagcatcga attcggcagg attaagcaaa atttcatcaa
     8401 actgaatagc tgtttcagca atatcagttg aacgctcttt cttgtttaca ttcgcatctg
     8461 ctttcttcac aagaattgga tatttgacat ctcctgatgt tcgcaccact gttccgtatt
     8521 tacgaagtaa attttcttct tgagcataag taataacttc agatgcaatt acttctggga
     8581 cagtaactga accgttgcca gcttcaatcc ctaaagctcg agcttctgct tcagaaatat
     8641 ttccagctac aaaattagca aatgcttttc gtagttgttg atctttttgt ttcttggtca
     8701 tttttgcacg tgcctccaat ccgttcttaa ttgatccaag taatccatct cgttgctgtt
     8761 gagtaatcat tccagaacga ttttctgaac tatcttcact gtctgaatct tcttcttgat
     8821 cgttgtcttc accagaacgg ctttcgtctg aatccgtact attcgattga tcatctgtat
     8881 tgtcgtctgt ttcatcagtt ccagaatctg cgcctaattc gtctttaatt ccgttcaatt
     8941 catcaataac actgtcaatt tcttctttca cggcttctaa atctgcttcg cgtaattctc
     9001 ctgattcaac tttttcacgt aattcagtca atcgttgctc acgacgtgcc ttcatttttt
     9061 tcaataattc tttatccatg tatttttcct cctacgcttc tagcgcttga ttaatttttt
     9121 tgattaattt ttttctttct tcgacgtttt tttctaattc atcacgactt cttaatgcag
     9181 cttctgtatc ttcgtatgct ggcaaaggca caatagaaac ctcgtataat tccacttcat
     9241 ggattgttcg aagcattggt tcagaattgt agtcccacgt ttcttcagtc ggcacaaaac
     9301 caaaactaca ttggttgatg tctcctcgtt ccatagattt aactaagtcc cttgctaccg
     9361 ttgtgtccgg taaatcaact tcgaatttca aaccacgttc atcttcttca aggcgaaggg
     9421 ttccgctttt agtacgtcct agcacattgg accaatcgtg attgaacaaa caacgaacat
     9481 ccgaattact aattgccttt gagaatgcac caggaacaat cacttcgctt aagtcatccc
     9541 ataaaagtgt ttgactgttg aatacagcag cgtagccgct aatggtcctc gtatgagttt
     9601 cttcatcgga acgtgttgaa agattggtga tgtcaatcgt gcgaatttcc ttcttcttca
     9661 tttccatcac ctcctttcaa gtcttgatca ttcgttggta aggaatcatc tgttgcattt
     9721 ttctggccaa tcctagataa gtcatttgaa atatagatag cttgtgtttc tggagtattc
     9781 tgtttaggaa aaccaagcat ttctgccacg ttatctggac ttgtaatccc ggtacgaacg
     9841 atgttgtagc caatatttgt ttttgttgag taaggaacaa aatccaaaat attaattttc
     9901 cattccactc tatagccaga attaggcata aaaaaaagag cggtgtaatg ttcgctcttg
     9961 tttttcaata ttggtttgat tgctttgttg tgcagataca tcatcgcttt ttcaatatct
    10021 gtcttcatca gcgattgata cgtatcaaca ttgattccta gaaattttcc taagtctttt
    10081 ttgtaaacac ctaaataatt caaaattgcc gcgtcatcaa caggactttt taatgtctcg
    10141 atggaatatc cttttcccaa aggaatcatc ttaacagaat gatcactctc attttgcgtt
    10201 ccttccagtt gatccaatat agctttgaca atttttgttt gggcgctatt atttggattg
    10261 atgtgggcgt ctagtttaag catgaacgca agtaaaccgc ctttagtata tttgtccgtc
    10321 aaaacttttt cagcgctcag aacgccttct agagtgtttc ttgcaagatc aattattcca
    10381 gcacctttta atgaatcagt tccgatgttc ttaatgtgtc gaatcatttg accaggtatt
    10441 ttttgaccat tcatttcaaa ttcttcttga agtcgttcat tgattttagt tgttacaccg
    10501 tacgccaaat gaagctgatc cctatccgtt aatgggaatg tctcaccatt gatcaataaa
    10561 gtatttgttt ccaatttggt aaattcgaat cctgtcaaat aatcattggg actcttcaaa
    10621 atatttagta agtgatgatc tttgacttcg ttgccgtcgg gaccaataac aattggtgtg
    10681 gccaatgcca cctgattaga tatgtcttga actaattcat aaacatcaga agattccatg
    10741 atagatgaat ccgtcacata tctttgacca taacgtgtgt agtgtccaaa aatatcctcg
    10801 atatatccac gtttttccat aaaagaatag actgcattcg ataaccgatc acgtaatttc
    10861 aatttttttc accgcctttc tatttatcta tagatggaac ttaggtaatc atctaactca
    10921 tctgaatcaa tatctgtcat ttgattcatc gtttccttat gaccacacaa aaacgccacg
    10981 aacccatcaa tctttttctt tgattgacgt ttacttggcg ctttttgtcc attgatgtta
    11041 gtaattgcta caacgttcaa agtgcaataa aggaataatg gattatcaaa ttgaattcgt
    11101 ttctcataaa acaaccgttc gacatcgtca aaaggagcat tcaatacttt aggatattga
    11161 gcaacttcaa cgcattccaa tcctaagttt tctaatttct caacaagttt gtcactcatc
    11221 gctggatcat aattcacttg ctgaatatca tataaattca tgcagtcctc tatgtactgc
    11281 aaaatctgat cttgatcaat catttttccg tcgcaaaatt caacgaaacc ttgttcagcc
    11341 aaatcactgt aaggaacatt atcttctttt tctcgaaact ctaaatcttc attcggaata
    11401 aaataaagtt gctttacttt aagaaccgct ttaccatctt catcccatgt tgggaagttt
    11461 aaagatacac aggtcaaatc tcgtgtacgt gataaatcta aaccgatata acatggttcg
    11521 ccacttaaat caccaagttc ttgtgtggta actaaacatg gctctacttg atcctgttca
    11581 aagaaattat ccgcgccgtt cacaaataca tccaagtgtt tcgttagaaa ttcagctttg
    11641 gaatgagctg accgttgcgc tgttttaaat gccgattcta aagcagataa atcgactgat
    11701 attccccagt taggattgca catttcccag acttttctat ccgtccaatc ataattttta
    11761 tttggctcat agattagaac aaagtttgaa tcattatcat cacgtttcaa cacttctttt
    11821 gcttcgcgat atacacgcat tccaacagac gacgaacctt tcccagcagt tgagatatta
    11881 aacatcaatg gctgtggtaa agatatctga gcagacttaa aattgtcata ctgttccatt
    11941 ttctcctgtt tatgcaattc atcgttcaat acaaaatatg gattggatcc ctcgatatta
    12001 tcaatattct tggtttgaac aataaattta tttgagtaag ccatatcttc atgaatatag
    12061 tcataagtaa tactagaaac agttcccttt ggccccctaa atattttagt tccttcaagt
    12121 aggattggat tgtttaagat tgtaactgca aaaggtttgg cagcatattg cgcttgagca
    12181 aaatcagatg cgcaagcata gcaatcaact gataacgccc cttctccgta cattgcatat
    12241 cctaatgaac cgacagcaat caatgtttta ccattctttt ttggaatctg gacataagcc
    12301 tcacgagtaa cgcgtacaat ttggcctttt tcattttctt taacccaacc ataaatccaa
    12361 gaataaataa atttttccca gggttctaaa agaaacggtt ttcccaccat ttctcctttt
    12421 gtgtgaacaa taaatgattc tacccaatcc atcatttcat tcgcgcgatc tacatcaaac
    12481 caaatatctt ttcttttttt ccactgatac cagcgatcta ttgctaaacg aacggtctta
    12541 ggatacttct taggtttctt tcttacttct tttgcaaata aatcagcata atttacacca
    12601 ggttcaatca tttttcagca cctgccttct tacgccattt atttctgtgc gctgctagtt
    12661 catctactgg ctttacttct gggcgtgtaa tttcttcatc ttttcttgcg gtcgatcctc
    12721 cagcaattag tctaccagtt tttgctttgt ttgttaatcc taataaatct agagctttca
    12781 tcttcttatc tgcccaagtt tctacttgtt gtgccaatgg atgttttgat tgattagtag
    12841 ctcctgattt attcgtgaat ttttgcgtct ccggaaatcc tttttccttc cacaataaat
    12901 atttgtgttg gtaaatttca aaaatatcca agtatgattc gattaatgga tcaagagtga
    12961 tggtgtacaa atcagacaaa ttcattattt ctaagatacg agctttttca gcacttactt
    13021 tttcatcaat aatcgcttta cgttgcgctt tagtcgtcat ttttgtatac accccccttt
    13081 gttttttgaa aaatttgacc taacgatgcg cgtgactccc cgctacccta tctccccacg
    13141 cgaaaaaatt ttgaaaatga ataggggggc ttcaattgaa atacgaagga aaaacttttt
    13201 tgtcttccgt ttcgttttca acaattggat gacattttga acataatagc atgagattgt
    13261 ttggatcaag cttaagcagt tcgttgtctt tgattggtac aatgtgatgg acgtgtgctc
    13321 tctttccaaa tatgaactga ccacaccgct gacaatgacc accttctctt tcataaataa
    13381 attgacgcat atctttccat gcttgcgttc gatagaatgg tttgttctca tgatgataaa
    13441 cagactttgc ttgctgcttc ttcttgcgtg atctgcttga tctcttgtgt tcagtacagt
    13501 agataccctt tgctattttg ttcgtgcatc cgtcaaactg acagtatttc attctacttc
    13561 acgaataaga tttatgatat cgccttttgc acggatagca ccaggaatat caataccatg
    13621 tttcttagca tatgcacgca attcttttgc agtcatattg tccagttcat ccgtatcttc
    13681 agtggactga tcattagtag cttcgtctcc atcaaaatta gcagcagtgt ttccatcgct
    13741 atcaagaatt tcactgctat gagctatgag ttcgccattg acagcaacaa atgatttgcc
    13801 atcactcaaa attccttttt cttcttttac ttcaaaattt ggttcttgat ctttcggaac
    13861 aactactgtc cttttctttt ctgaatccca atactctgtt ccactaatgg atgttctaat
    13921 tttgatcatt gccattttaa ttctctcctt tgtaatttgt acggattact tttgcgccca
    13981 ttcgctcata ccattcaatc gtttcctgca gattgggtag agtgtgtgac aataaagaaa
    14041 taactaacgt attcattcca gttctgtaat catcattctc cacttgcaca tgtgcatgat
    14101 taccattcca attttctctg tacggactat tctctgcttc aattgctttc ttataaactt
    14161 tagccaaact tgattctact tcaatactaa gtaccgcttc atgaaaatca ttcatttgtt
    14221 tactctccta tcagatattt tgtggtatga tttataaaaa agatgcgagg tgttttaatg
    14281 cttcaagaag ttaatattaa gtatcgtgat cgtcaaaata cagagacaat ttatttgcca
    14341 aatagttgtc cgcattgcgg aaatataatg actccgtatg tgtattttgg ggtttcatca
    14401 tatagtgcgt tcgataatga tagaatcttt ggcgtattag tacagtgtat aaatgatgag
    14461 tgcaacaaat tttatagttt acaatttata aatacgttta atgaagatga aggatattct
    14521 gaaaatattt taattaaaga ttattaccgt ccgccgatta aaatagattt accagaaaat
    14581 attgaaaaag tttctcctac atttgttgat atttatactc aagccacaca agctaaatct
    14641 gagcaattgg aacaaattgc tggagttggt tatagaaaag ctctagaatt tctaattaaa
    14701 gattatgcaa tatctaaaaa ttcagaggat gaagataaaa taaaaaaaat gttattaggt
    14761 aaaacaattg aaaattattt gaatgatttt cctaaactcc aaagattagc caaagctgct
    14821 acttggattg gtaatgatga aacgcactat gttcgtagac atactaataa agatgttcat
    14881 gatatggatg catttattaa atcagcagcg caatttatcg cggctgatta tgatgctgat
    14941 ttagctgatc aatttataaa tggaaataac tttatgccaa ctactaatta aaagaataaa
    15001 agagccacag ataccaatta ataaaacggt tgggatatct tggctctttc atattttttt
    15061 gacactaata gaatatcatg ctgctaacaa tgtttacata gctacaatga ccgaacattt
    15121 agtgaacagt gtaattttag aataaagaga gttgttcttt gtacttctcg ctattgtaaa
    15181 aatcaatttc gttttctttt ttcaatcggt cagcttgtct gtgttcatac tcatctaaaa
    15241 agttcaatgt ctttctaatt tctgcatgtc gctgtctgac atacgatgag ctatacccaa
    15301 cagattcagc aatatcttct aagctcattt gatcaacata tttcatctta acaatttgat
    15361 tatcgatacc agaaaagcta tctatcagca ataacatttc ttctttttgc tcgatcaaca
    15421 attccaattc attttctatc ttttggatat tctcttctaa agaagaagtt ctcgaattct
    15481 tttcgatgcg gacgtttgct aaatcaccac agacccatcg attcaattca agcttacttt
    15541 tattaagatt ccactttaag taaagaatct gctcatctag ttcttggtaa tcttttaacc
    15601 aatgaaatct cacaaacgcc accccttatg gtagaatagt attgcgctgc tatctcgaaa
    15661 ggggtagctt ttttattttt tcttagccgt tccattctgg attgcctttt gttccaaacg
    15721 tcgttttttc ttcttaatct ttgattttgt ttttcccata caattctcct ttccgaaaac
    15781 acattttgtt cgatttttaa tactaaataa aagttggcca gaatttttcg ttcgtcttta
    15841 ctcatctttt tttgaataag attactagca taaaaatagt tagtaccacg aaaatgaaca
    15901 ggtaaatcaa ttacgttcac cttcttcatg ccagttatca gccactagaa tctttgcgtt
    15961 agtgccgcca ccgttttctt ttttgtattt ttcaaatgct ttataagcag ctgtttttga
    16021 tttatagatt tttccggaac cgactgggaa catccatgtg ccctctgtga tagctaagat
    16081 gtatactttc atttaattca cccctttaat ccccaacact acatatccgg acttctgcat
    16141 gtaatcagta atataagtga tctctgcttt gtatgagtca ccagtgtaac taaatgttgg
    16201 tctcgttagt ccaggtaatt catgtttaat gtatttttta tattcacgta gtattaactg
    16261 atcaccaact ttgaagtcac gatcattctt gcggatctcg aattgtttgc gtccgctcgt
    16321 cactgcttca aaatattctg gtagtatttt tagttcgtgg atcattctgt ttcctccttc
    16381 aaaattcgct catcatcaaa aagtgaatct ccacaaaatc cttctcccaa atatttatcg
    16441 ttcatgtata agtcaaccaa atatttgtga tctgcttctc gatattcatt aatattaacg
    16501 atctttattt tgtaaacatt accttcgtcg ctcactatct tgaattgttc tctgactttc
    16561 accaccgttc ctcctctttt tgaaaaaatt ttagtgtttt tgtaagaccc cctaaatcag
    16621 cttttttatt tgata
//
LOCUS       DACCXM010000057        16384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCXM010000057 DACCXM010000000
VERSION     DACCXM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.059"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..640)
                     /locus_tag="IVY61_001606"
     CDS             complement(<1..640)
                     /locus_tag="IVY61_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970721.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLY"
     gene            complement(819..1538)
                     /locus_tag="IVY61_001607"
     CDS             complement(819..1538)
                     /locus_tag="IVY61_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7970722.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1669..2820)
                     /locus_tag="IVY61_001608"
     CDS             complement(1669..2820)
                     /locus_tag="IVY61_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7970723.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3037..3777)
                     /locus_tag="IVY61_001609"
     CDS             complement(3037..3777)
                     /locus_tag="IVY61_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7970724.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3781..4104)
                     /gene="rsfS"
                     /locus_tag="IVY61_001610"
     CDS             complement(3781..4104)
                     /gene="rsfS"
                     /locus_tag="IVY61_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP7970725.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4161..4754)
                     /locus_tag="IVY61_001611"
     CDS             complement(4161..4754)
                     /locus_tag="IVY61_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7970726.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4738..5385)
                     /locus_tag="IVY61_001612"
     CDS             complement(4738..5385)
                     /locus_tag="IVY61_001612"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP7970727.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5398..5709)
                     /gene="yhbY"
                     /locus_tag="IVY61_001613"
     CDS             complement(5398..5709)
                     /gene="yhbY"
                     /locus_tag="IVY61_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP7970728.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5725..6834)
                     /gene="yqeH"
                     /locus_tag="IVY61_001614"
     CDS             complement(5725..6834)
                     /gene="yqeH"
                     /locus_tag="IVY61_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP7970729.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6831..7358)
                     /locus_tag="IVY61_001615"
     CDS             complement(6831..7358)
                     /locus_tag="IVY61_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP7970730.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7538..8323)
                     /locus_tag="IVY61_001616"
     CDS             complement(7538..8323)
                     /locus_tag="IVY61_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP7970731.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8316..9185)
                     /locus_tag="IVY61_001617"
     CDS             complement(8316..9185)
                     /locus_tag="IVY61_001617"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP7970732.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9186..10565)
                     /locus_tag="IVY61_001618"
     CDS             complement(9186..10565)
                     /locus_tag="IVY61_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP7970733.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10573..10992)
                     /gene="fabZ"
                     /locus_tag="IVY61_001619"
     CDS             complement(10573..10992)
                     /gene="fabZ"
                     /locus_tag="IVY61_001619"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7970734.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11017..11493)
                     /gene="accB"
                     /locus_tag="IVY61_001620"
     CDS             complement(11017..11493)
                     /gene="accB"
                     /locus_tag="IVY61_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP7970735.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11496..12731)
                     /gene="fabF"
                     /locus_tag="IVY61_001621"
     CDS             complement(11496..12731)
                     /gene="fabF"
                     /locus_tag="IVY61_001621"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP7970736.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12750..13487)
                     /gene="fabG"
                     /locus_tag="IVY61_001622"
     CDS             complement(12750..13487)
                     /gene="fabG"
                     /locus_tag="IVY61_001622"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP7970737.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13499..14416)
                     /gene="fabD"
                     /locus_tag="IVY61_001623"
     CDS             complement(13499..14416)
                     /gene="fabD"
                     /locus_tag="IVY61_001623"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7970738.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14523..14747)
                     /locus_tag="IVY61_001624"
     CDS             complement(14523..14747)
                     /locus_tag="IVY61_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7970739.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14800..15762)
                     /locus_tag="IVY61_001625"
     CDS             complement(14800..15762)
                     /locus_tag="IVY61_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP7970740.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15772..16212)
                     /locus_tag="IVY61_001626"
     CDS             complement(15772..16212)
                     /locus_tag="IVY61_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7970741.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4326 a         3727 c         2794 g         5537 t
ORIGIN      
        1 tatataaatc caaatctgtt tcaggatatt ttaatttgac aagcgaaata atcattttca
       61 gttcttccat gttttctgga tattctgggt caaatacctt gctatgacgg ccaatcccgc
      121 cattgtcgat caatcggtaa attcgattcc attcaatgaa ttggtcaatt gattggaaat
      181 tgctgattgg atagccgcca caatccaaaa tctctgatac acgaaatgct aggtatgcac
      241 tatcgatcac tttatttttt ttcgtgtcta tcttctcatt tctttcgata agaatttttt
      301 caggacgatg tttcaagctc cactctaata gtagaaagaa agcctccatc cgatactctt
      361 cttcaaataa ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc agataatttt
      421 gtctataaat cagcattggt gcgcggtcca ccatacactc taaataacgg ttcagatacc
      481 ctgtaccgac atagtttcct aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg
      541 ctaattttga acaatcagtt acgttcacag ctaactcttc tactttgcgg taaccgctta
      601 agatacgttc aaaaggttct aaagtgattt ttgcttccat attcttcccc gtccatcttc
      661 cacatacttc atatcatttc acatgatact tatgctctca tcataccaca ggttgaatta
      721 gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt
      781 tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa tgccacatta
      841 tggaacacat gctgtacatc ttctagatct tccagtgcat caatcatttt ttcaaatgtt
      901 tcttgatctt ctggagatag tgttacctcg ttttgaggga tcatttggat ctcagcgatt
      961 gaaaaatcag tgattccttt ttcttttaat gcttcttgta tactatggaa atccgctact
     1021 tcaccgtaaa cgatgatttg tccttcttct tccattacat cgcgaacatc gatgtctttt
     1081 tccattaggt attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta
     1141 ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt tcccgccatt tttcccaaat
     1201 gctgcccgta cgtctgctgc tgttcgattc acattgttcg tcaatgtatc aacaatcacc
     1261 attgagccgc ttggtccaaa tccttcatag cgcaattcag agtatgtttc atctcctgca
     1321 ccttttgctt tttcgattgc tcggtcaata atatgttttg gtacattata agttttagcg
     1381 cgatcaatga cgaaacgtag tttttggttt gcatgcggat caggatcacc tgattttgct
     1441 gccgcgtaga tttcaatccc aaattttgca taaatccggc tattattagc atctttagca
     1501 gttttctttg ctacgatatt tgcccattta cgtcccatat tttcacttcg ctttctgatt
     1561 tgatagtttc ataacgcaaa aacgtcctgt tccattatac ttgtgaatca cgattctgtg
     1621 aagctttttt tctttcttcc ttcattcttc tcatttttag tcaattatct atactcggat
     1681 tgggatttgc ccgaaattct gttccggaat attgcctccc aattgataga tttgatcact
     1741 tctaactaac agttccgctt cttgtgcctc tttttttccg attcgggaat aaatagaaat
     1801 ctcttttttt tcttttaaga atcttcttcc agcatcggta aatccaagta gtcgaagata
     1861 atttttattt tgttggacag gaatctcttg ctttttgata ttcagcaaga cataacaaga
     1921 taatcgttgg atccgtgtcc atgtatatct ttttgttttt acctttgaca caaaatcctc
     1981 aaaactatca gcagttgcag ctgctgtttt catgcgcgat tctattcctt cggtcatctg
     2041 atagatatct gtcagttcca gcaaagtaga agataacaac cgataacgca aatacggcca
     2101 ataagattcc caagtctgca gcgaacaagt tgaaagatct tcttctgtct gaggcggcac
     2161 aagtccagct attctttttc cttcttctaa cgcatggcga atggctgttg cactggctat
     2221 cctctgatct gtgatgattt tttcatgata gcctgcactt ttacgtcgga tcggatgtaa
     2281 ttccataggg tggacatact tggcattttc ctttgcataa cttaatccca agatatgatt
     2341 tggcgaagaa aaatcaagcg taatatcggg atacagactg cgaaaaacct cagtcatttt
     2401 ttgcggataa ctatacgttt gatcagacag ttcttgaaat gcctcttcta tcctatcttg
     2461 attctcttgg acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt cttctgttcc
     2521 aaaacacaaa ctgtcacagt ccagtgtttg caagattttt actgcacctt tagcaaaata
     2581 gtcagctgac tgaaccgccc attcgacggg aagctccact actaaatcca caccattttg
     2641 taaagcttca ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa
     2701 attcccgctc attacagcaa tgacgacctc tgctcctgtt atttttcgtg ccatttccac
     2761 atgatagcga tgtccattgt gaaaaggatt gtactctacg ataatcccgc agcttttcat
     2821 gcgcatcctc cttcatagtt atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa
     2881 aatcggaaaa aatacccgaa ctatataaac tattctcgca aacacaatac ttctgcgacg
     2941 aaaagctgca ctgcaagagc aatgcctata tcgttcggat atataattta tgttgaagat
     3001 ttattctttt cttgttaact cgagcaggca aaaccgctag tctttgtatg ctacaaagaa
     3061 ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt
     3121 cgaaaagccg ctgttttcca gcattcttaa ataactttcc aatggatagg tacgctcttg
     3181 atgtagttca tcttcacgaa taaatcgacc agacccttct tgttcttcaa cgaaaaaggt
     3241 aagaaaatgt tcgatgctgt gtttctcgct tcctggataa ctatcccaca aaaaagcaaa
     3301 attatcccct tgataatgat agctatattc tggaaaaacc tcatctactt gataggtgga
     3361 gtgtacgtca aataaaaatc ggccattttc ttctaacgct tgatagactt catcaaacac
     3421 ttgctgtacc tcttgtctat ttttcatata gcacagcgaa tcagaaaaac aggtaattgc
     3481 ctgatattgt ccgatttctg ataaatcgag catattgcct tccacaaatt ggatcgatgc
     3541 gtcttcttca tatgctcgct ggctggcaat catcaacatc tcttctgaaa ggtctaaccc
     3601 tgtcacttta tatccatctt ttgcaaaggc aaccgctaat gctccagtac cgcatgccag
     3661 ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta accattgctg
     3721 ataaagtgtt tcgtccatca cttcatcgta aatgaaagca aatgtttcat aagccatatg
     3781 ttaatccaac cattcactaa gatcgactag cggagcatct gaccatagtt tttccagatt
     3841 atagaaattt cgttctggcg catggaaaac atgtacgatc acatctccaa gatcgatcaa
     3901 tacccatttt ccgccttctt ttccttcaat acgttttact tcatatttgt tttcttcttc
     3961 tttatcaatg atttcttctg taattgcgtt gatttgacgt tcgctatttg ctgaacagat
     4021 catgaaataa tctgccaaca aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc
     4081 ccgttttgaa tctgctgctt caatcgcaat ttttaattgt tctttactat ctatgatgat
     4141 ttcctcctaa tatgagcagc ttattttttt gctacccagt aattatatgt ttcgattgtt
     4201 tttggataaa tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat
     4261 gctacagctt cgtctagatc agcccatgca atggctcgtg catcttgtac cccaggaaaa
     4321 tctcgacctg gttcaatata gtctgctaca taaatgattt tgtcaaggag actcatctta
     4381 gcagccccag tcgtatgcac ccggattgca tgaagaatct ctgcatcggt gattcctaat
     4441 tcccgctcta caaacgaagc gccaacaaga ccatgccaga ttgcattatt ataattcaat
     4501 aactccggat caaaaccgtc tcttacgatt accattttga actcctcatc tggtcgttct
     4561 tttgcataat catgtgtcaa tgcagcaata ctagcttttt caggagaagc accgtatttt
     4621 ttggcaagag ccacggctgt ttcttctact ccaagcacat gcttgaagcg ttgttcgctc
     4681 attctcatct gtattttttg catcaggttt tctctagaat aagcagtata ttcattacta
     4741 tagtccatat tcatacaacc cttcattttg aatataatca attactttat ctggaacaag
     4801 gtagcggata gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat
     4861 ctcaggtaca tctacccaaa ttacaggata tggagtatcc gttgtgtatc ctggtcgacg
     4921 tatacccaca aagttgacca tagaagtcag ctcatcgatt ttataccatt ttggcaaata
     4981 ttctaccata tccccaccga taataaagta ataatctgta tccggattgt tttgcgtcaa
     5041 ttccttcata gtatcatagg tgtaactttt cccaccacga gctaattcaa tcgtttcaat
     5101 ctgcaaaaaa ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc
     5161 aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc
     5221 aagacctaaa ttccggccag cttgttcagc catcactaaa tgtgcaaggt gaacgggatt
     5281 gaatgttccg cccaagatcc ctacttgctt tttcttctct aagaattgag gcatttcttc
     5341 aggaaataat tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg tcactcctta
     5401 tattgctttt acttcttttg atagcctttg atacttttct tttgtggaag gtttgtataa
     5461 tacaataaca cgtccaatga tttggacgat ttcgcaatct atttcttttt ctaatacttg
     5521 agctgcttct tctgcaatct catctgtatt ttgaagtaac gatactttga tcagttctct
     5581 tttttctaaa gcttcttcaa tttgaacgat cattgctgaa ttgatcccac cttttccgat
     5641 ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt gttttcctct
     5701 taaactcata attttccttc ttccttaaat caatgctttg cgtttcaata catctacacc
     5761 ctttggcgcc cagcccgcaa ttactactgg cgctgttaca gtgatccagc ctagaccggc
     5821 aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc
     5881 ctcgacttca tcaggacgag gaggctgtaa taatccacct acatgttttt gataaaattc
     5941 gtctgctttt tccagtttcg ttcgatgaat atttacatca tttgacacat aagcaataaa
     6001 tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg
     6061 cccttcattc agctgataaa ctttaggttt gatttctttt aacggtgcaa tgatccgcaa
     6121 atctttttta cctaagtagt gcgccatttg atggcgatgg ataattcccg gtgtatcaat
     6181 caaaaaatgc ccgtcatcta aaggaatctc gattttatcc aaggtcgttc ctggaaactg
     6241 cgaagttgtg attacatctt gtactcccgc tgtatttttg atgatttgat tgataagcgt
     6301 cgattttccg acatttgtta ctccgacgat atagacatcc cgtccatcgc ggtattcttc
     6361 gattttttct aatagttccg tcatctcatg agatttctta gcactagtta ataatacatc
     6421 tactggacga attcctgctt cgtaagcacg ttccttcatc cattgtgtta atttagtttt
     6481 tttcaatgat ttaggtaata tatccacttt gttcccaact aaaagtactg gattgtcacc
     6541 gacaaaacgg tgtagtcctg ggattagtga accattgaag tcaaaaatat ccaccacatt
     6601 gacgatcaat gcatcttttt gccctaattc attcagtaag cgcaaaaagt cgtcatccgt
     6661 caatgagaca tcttgggtct cattataatg gcgtaatctg aaacaacgct gacaatagac
     6721 ttccccggaa gctaaacctt tttccaaagc cgtttgcggc gtgtatccta attcaccggg
     6781 tttgtttgtt tgtataacgg caccacagcc gatacattgt aattcttctg tcattcgatt
     6841 ccacctcgcc agcccatatc agggtgtttt gctaatagat aggccataat tcttttttcc
     6901 caaaaacgat taaattgtgt cttccaacta tctgttgcga taatgggttt tacaaggatg
     6961 ctacgtatcc ctgcacgatt ggctccacgt atatcagtca taatctgatc gcctaccatt
     7021 actatctcat catctgctaa atccaattgt ttctttgcta tgttgatccc tcttgctaaa
     7081 ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg cgcgacacgt
     7141 tttgaattat tgtttgaaac aacaacaaca gggattcctg catttttcat ttctaaaatc
     7201 caagtaagca actcttctgt tccatctgga ttattccatg caatcaacgt attgtctaaa
     7261 tcggtcaata ctgctttgat gcctagattc tttaattggg caggtgtaat ctgatagatg
     7321 gcttctacca tccatgttgg tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa
     7381 atgaaggagc acataaagta ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa
     7441 ctacctctat actcgttcgt ctttcagaaa aagcatttct ttacaaaatg accaaaaata
     7501 aagattgatc acagaaaaat ttagaaacaa aaaacaacta atattggcgg aacctcttgt
     7561 atcgtttttc cagtaattta tgctgatcta atcgaacaag ttccgtcaat ctttcaatca
     7621 acgctttttt cataatgtgt gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt
     7681 caggaataac tcgatcaacg attcctaact cgcgtagttc tggcgcagta attttcatca
     7741 actcagctgc ttctgcggcg cgactaccgt ctttccacaa aatcgacgca aatccttctg
     7801 gagaaagcac agcataaatc gtttcttcca gcatccagac ttcatccgct aaacctagag
     7861 caagggcacc gccacttcca ccttcaccaa tgatgatggc gatgatcggt acctttaaat
     7921 cagacatttc gagcagattt ctggcaatag cctctccttc gccgcgctct tctgctccta
     7981 caccgcaata tgcacctgct gtattgatca aggtaatcac cggtctattg aacttttccg
     8041 cttgtttcat cagtcgtaaa gcttttcgat agccttcagg atggggagaa ccaaaatttc
     8101 tgcggatatt ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt accggtttgc
     8161 catctaatcg agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat
     8221 ggcattcctc gaaagaatcg aagatctctt caaaaaaatc caagcttgtc catcgttctt
     8281 tagctcttgc taactgaacg acatcatgtg ctgtcttatc cacgatctat ccatcctttc
     8341 tgtgtatgca attcaagaag cgtatggatt ttctgtttca attccatgcg gggaacaatc
     8401 tgatcgacaa aaccatgaga taataagaat tcagcctttt ggaaatcttc tggaagttcc
     8461 tgcttgatcg tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct ttggggttct
     8521 gctagaataa tatcgccttc cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc
     8581 gttaaaacag ttagataaaa aagaccttca ttgctatgcc gtttgacagc agcagagatt
     8641 tttgccatct gcattagaga aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg
     8701 aacaagacga ctggaagact ttcctttgtt gctcgttcga ataatctggt gatcttttct
     8761 ccgacaatcg ttcccatact tcccatgata aaattaggat ccatcactcc aataatgaag
     8821 ggaatcccct gaatggttgc tttaccagtg aggactgctt catgaagtcc tgttttatct
     8881 tgcatttttt taattttttc cgtatattcg ggaaatgcta gcggatcttt ggttacaaga
     8941 tccgtatccc attcttcaaa acttttttca tcaataatca aagctagacg ctgccatgca
     9001 ccgattcgaa aagcgtatcc gcaatgaggg catacttttt cttcaccgat gtctttcgta
     9061 taaaggatat gtttgcaatt tggacacttt gcccacatgt tatcaggaac agatggcgag
     9121 ttagcaggtt tgctctctct attaggattg atccgaatat atttcttttt cttaaacaga
     9181 gccatctatg cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt
     9241 aaaaatgctg tgctgtaatc acctgctatg actgcaggat gactaatcaa atccatctga
     9301 aattctgcgt tcgtgatgat tccttctgtg ataaattcac ttaaggctcg ctgcatcttc
     9361 attaatgctt caaaacgcgt atttccatgg acgattattt ttgcaatcat tgaatcataa
     9421 tatggcggga tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc catcccacct
     9481 gctggaagca gtaagttttg gatcttccct ggagaaggag caaaatggaa agcaggattt
     9541 tctgcattaa tccggcattc gattgcgtgt ccttggaata ctacttcttc ttgacacaca
     9601 ttcaaaggct caccggctgc tatctccacc tgttttttta ctaaatcgat tcctgtgacc
     9661 atttctgtta ctggatgttc cacttggatt cgagtattca tttccatgaa ataaaaatcg
     9721 ccttcttcat ccatcagaaa ctcgatcgtt ccggcatttt cataattgac tgctttagct
     9781 gctcgaacag cagcgtctcc tagtgcttct ctttttgttt gtgagatcac tacagaagga
     9841 gattcttcca gtactttttg attgttgcgt tgtaaagaac aatctcgctc gcctaaatga
     9901 atgacatgac caaatcgatc acctaagatc tgaacttcta tatggcgagc aggataaatg
     9961 attttttcca aatacatatc atcatttcca aatgccgctg ctgcttcttg ctgagctgaa
    10021 gaaaaatgct gtgggagctc ttcttttgat tgaactttcc gaatgccttt cccgccacca
    10081 cctgcagctg ctttcagcat cactggataa ccaattctgt ctgctatttc caatgcttct
    10141 tctactgtag ataatacacc agtacttcct ggaatgactg ggacatttgc ttcttgcatc
    10201 aattgacgcg cattgatctt atttcccatt gcatcgatcg ttgcactttt aggaccaata
    10261 aatgtgatat tgcactcttc acacatggag gcaaatcggc tattttcaga taaaaaacca
    10321 aatcctggat gaattgcttc ggcttttgtc acgattgcag cgctcagtac ttcctgaaca
    10381 ttcagatatg aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt
    10441 gtatgaagtg cttcggcatc tgcttctgaa tatatcgcta cggtttggat ccctagttcg
    10501 cggcatgcac gaatgatacg tacagcgatt tcaccgcggt tagctatcaa tactttagaa
    10561 aacatatttc acctatccaa tcataaaggt taattccgct tcggctactt ttttaccgtc
    10621 tactttagcg attcctttgc ctattccggc tgaagctttc actttcaata tctcaacttc
    10681 aaggtataag gtatctcccg gtgtaacttt cttacggaat tttgctttgt ccaatccgcc
    10741 gaaataagcc gtctttccct tgaattgttc aagagaaagt aatgcaactg ctcctgcttg
    10801 agccattgct tcaacaatca atacccctgg cataacaggt tcttctggaa aatgtccttg
    10861 aaaaaagggc tcattgatcg ttacattttt cttagcaacg atccgttctc cttcaaccat
    10921 ttcttccact cgatcaatca aaagcattgg ataacgatgt ggaatgattt cttttatttc
    10981 ttgtatgttc attgttttcc ctctttcctg ctttgcttat ttttgctgaa tcctaaagag
    11041 tggctgtcca tattcgacaa ctgactcgtt ttctacaagg atttctgtga tcactccgtc
    11101 ggcctcacta gttatctcat tcatcagttt catcgcttca ataatgcaga taacttctcc
    11161 tttggcaact tgatcaccta cttttttgaa agctggtttg tcgggcgctg gttgcagata
    11221 aacgattccc acgattggcg aaacaacctc gattccatca aatactgccg attctttttc
    11281 tttcattatt tctggagcag ttgttgcttg ttcaggtatg acattgactg gctccgaaac
    11341 tggcaacgtt tcgtttgttt gaaccatatt catgcctcga cttgttttat ttttattcat
    11401 atacaattca aattgacctt cacgcaaatc gaattccgtt agcgaggatt gatcaaattg
    11461 actgactaat tctctgattt cattgatatc caaaattatt cctcccagcg tttcatgcac
    11521 agaaccgcat tatgtccgcc aaaccctaat gaattactca ttgcataagt aaaacttgca
    11581 ggtttgcttt cgtttttgac aactgtcaag tcaatctcag gatcttgttt ttctacattg
    11641 atcgtaggtg ggataaattg atgttctaat gccagcaaag ttgccaaggc ttcgatcccg
    11701 ccggctgctc ccaataaatg tccagtcatc gatttcgtgc tgctgacgta agtatctctg
    11761 aaattttcac ctaatgcgat ttggattgct tttgcttctg cctggtcatt ggctggtgta
    11821 cttgttccgt gagcattgat atatccgact tgttccggct gtacaccagc ttcttccatc
    11881 gcaagcttca ttgctttaga tgcaccagaa ccatctggag taggagctgt catatggtaa
    11941 gcgtcacagt ttgcaccgta tccaacgatt tctccataga ttttggcacc gcgagcttgt
    12001 gcatgttcta aagattcaag aactagaacg cctgctcctt cacccatcac aaaaccattc
    12061 cgattttcgt caaacggaat cgaaccacga ttaggatcag ttgattctga gagtgctgtt
    12121 aaagctgcaa atccagcaat ccccatctca caaatcgttg cttctgttcc tccagcaaga
    12181 atgacatcag aatagccatg tttgatgtta cggaatgctt ccccaattga attcgttccg
    12241 ctagcacaag ctgttacgat agctgtacaa ataccttttg cacctacacg caaagcaata
    12301 tttccagctg ccatgttggc aatcgccatt ggtacgaaca ttggagaaac tcgttttgca
    12361 cctttttcat gtagacgaat aacttggttt tcgattgttg gaagaccacc gatcccagat
    12421 ccgatgatga cgccaaaccg gtcttgattc attttttctt tatctaaacc gctcatctcc
    12481 attgcttcta aagcagcgta gattccatag atcgaaaaca tatccattcg tttgctatct
    12541 tttttaataa aatatttatc gtaagggaaa tctttgactt cacccgctaa ggtaatccct
    12601 gtttctgaag catcaaattt agtgatcggt gcgatgccat tcgtcccctc ttttaagcta
    12661 ttcaaaaagc tgtctgcgtc atttccaata ggtgaagtga ctccatagcc agtaattacc
    12721 actcgattca tttgtttgct ctccttttct tatccgtgca tgaccaatcc gccatcgaca
    12781 ttgatgactt gtccggtgat atatggattt tgtgctaaga aaactgctgt ttgtgcaata
    12841 tcttctactt gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc cttgactttg
    12901 tctgctaaaa cttccgtcat atcagttgtg atgaatcctg gcgcaatggc attacatgta
    12961 atgcctctag ttgcagcttc tcgtgccaca gattttgtta atcctactac gccagcttta
    13021 ctagctgcat aatttgcttg accaatattt ccaatcaacc cagatacact agagagatta
    13081 atgatggccc cttctcgctg cttcatcatt tttttcaata cgtgctgcgt catattgaag
    13141 gttccaatca aattgatatc cagacatttt ttgaaatctt ctgcatccat tcgcatcaca
    13201 agtttgtcat ttgtgattcc ggcattgttt actaacacat ggatcgatcc aagcttttct
    13261 tctgcttctt tgatcatttg tcccgctttc tcataatcag agatgtcacc ggaaactcct
    13321 acgcatttta caccgaatgc ttcgatttct tctatctttt cttttggaat ctctccgcga
    13381 ccattcaaaa tgatatttgc tccagctttt gcaaaagcta aggccatcgc tttgccgatt
    13441 cctcttgtac tacctgtgat aaatacattt tttcctttta catccataac gttcctcctt
    13501 atagtattgc tattgtttca tcaaatgtct gcttattctc aacacgcaga actggtattg
    13561 ttttatcgat ttttttcata aatccgctta atacttttcc ggggccgact tcgatgactt
    13621 gttcgatgcc gattgttttt aacttatgga tactctcata gaaacgaaca ggttgcatga
    13681 cttgctgttc aagtagtccc gcgattgttt ctttcttcat tatttccgtt gttgtgttgc
    13741 ttatgatagg aaaagaaggt tcagagaatt gaatctgact taggtccttc gccagttttt
    13801 ttgctgccgg ttctaaaata gctgtatgaa aaggcccgct gacatttaga gggatcattc
    13861 ttttgaaccc tttttctttt aatagtaaaa cagcttcgtc tactgctttt tcttcgccac
    13921 caatcacgat ctgctgaggt gtattataat ttgcaggaga tacgatgccg tacttgctag
    13981 cttcatgaca gctttcctcg atcgtttcaa tcggcgcatt catcactgca accattttcc
    14041 cgcttccagc tggggcagct tctgtcatat aagcgcctct ttttgctact aacgcaactg
    14101 cttcagtgaa ggatagcgca ttactagcta ctaatgccga atattctcct aaactcagtc
    14161 ctaaagctgc atctgggatg attccatgct ccttcaataa gcgataatac gcgatgctga
    14221 ctgttagaat agctggctgt gtatattgcg tctgatccaa acgttcattt tctgtaaagc
    14281 aaagttcagc catatcgtag ccgagaatct cacttgcttc atcaaatgtc tgctttacaa
    14341 cggcttcttc atataaatct tttcccatac cttgatattg tgcaccttgg ccactaaata
    14401 agaatgctgt tttcatgttt ttctccaata tatccagaat atatgtttga ttcttcctat
    14461 tcaaaaaggc cgagacattc ctatttcagg aaatgtccca gcccccaacc taattttaat
    14521 gattatgcag tttgtttttc tacgtaagtc actaaatctt gaaccgtttt gattccgtct
    14581 tctgtttcga ttttcacgtc aaattcatct tcaatttcat tgatgatttg gaataaatcc
    14641 aagctgtcag cttctaagtc ttcttggatg tcagtagtca atttgatttc ttcagtttct
    14701 tttcctaatt cttctgcgat gatttcttga attttttcga ataccataat aatatattcc
    14761 tccgatattg tttgcttttt atttttataa tttaacagtc tacaactcca tgagaatact
    14821 tccccatgtt aaacctccgc catagcctgt aaatattact ttctgctgtt ttcctaaggt
    14881 gatctttcca ctagaaactg cttcatccaa taagataggg atgcttgcag cagatgtatt
    14941 cccatattta tccatattgg tcaggaactt ttcttgcggc atctttactt ttcgggcaat
    15001 tttctcaata attctgaggt ttgcctgatg caaaagcaga taatcaactg gtttgtcttt
    15061 catgacttct tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc
    15121 agtcatttgc aaataatacg aaccttctga atcttctgaa tagaacggat ttttattttg
    15181 ctggtagcca gcggtcaatg agctacttct ggtaccgtca gctgctaatt tttctttcag
    15241 caattttttc tgtggattcg cttctaagag aatcccacct gctccatcgc caaacaacac
    15301 agcagtcgta cgatcagacc aatcgattag cttagatagt gtttcaccac caatcaccag
    15361 tcccttagtt gagccgcagc ggatcatttt ttcagccatg cttaacgcat aaacaaatcc
    15421 tgagcacgca gcactgatat caaaagcgat agcatgagtt gctcccaatt tcccttgtac
    15481 ttgtgctgct acagatggtg tcgtgtaatc gggcgacatt gttgctacta aaataaaatc
    15541 caaatcttta gggtttagat ttttctgttt tatcaatttt tccgccacta aaatgcataa
    15601 atcagaagtc tcctgatttg ttacacatct tcgttcacga atacccgtcc ttgacgaaat
    15661 ccattcatca gaggtatcca tcatcttgga aagatcatta ttagtaataa tcttttcagg
    15721 aaggacgctg gctgttgcag taatcattcc aaattgttcc attaaatatc ctcacttata
    15781 gtcttgcaag aagtcgtgca agtttttcag tgcatccagc aaggcatgct gttcctccgt
    15841 agccattccg tctaagatgt tcttgaccat ttcacgatga aaatgctgat ggacccgaaa
    15901 tagtaacttt ccttttttcg ttagtcccaa ctttacaacg cggcggtcat cttcactgcg
    15961 gatacgttcg acatagcctt tttttactaa cttgttaata gcagtggtca aggtcccaac
    16021 agtaattgat agttctttgg ctacttctga agttgttttt ttcttgtaca tcccgattgc
    16081 ctcgatcgtg tgcatctctg tgattgacag atctttgaat tgagattttt tcagctcaga
    16141 ctcttcgatt gtcaaaatat cattgaagac actaacaaga taatcattta ctgtttttaa
    16201 atctggttcc atgttccacc gtccactaat tagtttgatt atcaaatctt ttgatgttca
    16261 aactatatct taactttttt caaaatgcaa ccttaaaaga tcattttttt actatatgag
    16321 ctttttttaa gattttactc atttaaaaca actgataaat cagcatttat aatttctttt
    16381 atta
//
LOCUS       DACCXM010000058        16266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCXM010000058 DACCXM010000000
VERSION     DACCXM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.060"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            102..1529
                     /locus_tag="IVY61_001627"
     CDS             102..1529
                     /locus_tag="IVY61_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7970742.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1621..1986)
                     /locus_tag="IVY61_001628"
     CDS             complement(1621..1986)
                     /locus_tag="IVY61_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7970743.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1983..2348)
                     /locus_tag="IVY61_001629"
     CDS             complement(1983..2348)
                     /locus_tag="IVY61_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7970744.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2540..2788)
                     /locus_tag="IVY61_001630"
     CDS             complement(2540..2788)
                     /locus_tag="IVY61_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970745.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2787..2915
                     /locus_tag="IVY61_001631"
     CDS             2787..2915
                     /locus_tag="IVY61_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP7970746.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2972..4666
                     /locus_tag="IVY61_001632"
     CDS             2972..4666
                     /locus_tag="IVY61_001632"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP7970747.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4766..4939
                     /locus_tag="IVY61_001633"
     CDS             4766..4939
                     /locus_tag="IVY61_001633"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970748.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5317..6450
                     /locus_tag="IVY61_001634"
     CDS             5317..6450
                     /locus_tag="IVY61_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP7970749.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6481..6801
                     /locus_tag="IVY61_001635"
     CDS             6481..6801
                     /locus_tag="IVY61_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970750.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6830..7294)
                     /locus_tag="IVY61_001636"
     CDS             complement(6830..7294)
                     /locus_tag="IVY61_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7970751.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7426..8679)
                     /locus_tag="IVY61_001637"
     CDS             complement(7426..8679)
                     /locus_tag="IVY61_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7970752.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8708..9025)
                     /locus_tag="IVY61_001638"
     CDS             complement(8708..9025)
                     /locus_tag="IVY61_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7970753.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9051..9371)
                     /locus_tag="IVY61_001639"
     CDS             complement(9051..9371)
                     /locus_tag="IVY61_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7970754.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9835..11250)
                     /locus_tag="IVY61_001640"
     CDS             complement(9835..11250)
                     /locus_tag="IVY61_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7970755.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11267..12583)
                     /locus_tag="IVY61_001641"
     CDS             complement(11267..12583)
                     /locus_tag="IVY61_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7970756.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12733..13431)
                     /locus_tag="IVY61_001642"
     CDS             complement(12733..13431)
                     /locus_tag="IVY61_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP7970757.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13720..14709)
                     /locus_tag="IVY61_001643"
     CDS             complement(13720..14709)
                     /locus_tag="IVY61_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP7970758.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14789..15622)
                     /gene="msrA"
                     /locus_tag="IVY61_001644"
     CDS             complement(14789..15622)
                     /gene="msrA"
                     /locus_tag="IVY61_001644"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7970759.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15797..>16266)
                     /locus_tag="IVY61_001645"
     CDS             complement(15797..>16266)
                     /locus_tag="IVY61_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970760.1"
                     /translation="NFNQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENK
                     EHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVK
                     EQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         5062 a         3278 c         2763 g         5163 t
ORIGIN      
        1 catttatatg ctttttcaga aattaactga tgatttcaaa aagaaaacat gtcataatat
       61 ttcccatatt gtgcaaaaaa atacaaggcg gaggctctac tatgattgaa gaatatattg
      121 agaaaaatat tttaagacag ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca
      181 atctagaaaa gctctccaat ctcctgcatg tttgcaaaac cactttactc aatgatatca
      241 ataacataaa aaaagaattt gaagaacaga tcgcttatac acatcgggaa aaggaccgtt
      301 acactttata tttttcagaa catatacctc gatgcaagat catgcagcaa ttgtctcaaa
      361 attcgttgtt tttaaaaact tgtctgcttt atttggaaga agatgaacct gattatcttc
      421 aattaactga atgtgaattc atctctgtca gcaaagctta ttcattaaaa aaacaagtat
      481 tagcttattt taatgactgc gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa
      541 tggaaagacg tctgctgctt cttaatgtgt cgtatcgttt aggcggtttt aattcttggg
      601 agctacctga atcgtttttt gaacgtgccg atcgtttcat tgaatccgta acggaaaata
      661 gtggcagatt ttatgataaa gaaaataaag aaatcttatc gattggtttt gctatcagtt
      721 tcctacgcca gcaagtgtgt gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa
      781 aacgtcctat ttacaattat gtcgaatcag catgggaaaa cactgatttc caaacctatt
      841 ataaaaaaga agaattcgcc tttattttga cactattcaa tctttgtaat tatggtttcc
      901 attcttatca gttgattgcg gaagactttc aacagctaca ccaagtgttt attgacaata
      961 ctcccgaaat aaaagaacta gttgctacct ttgaatctca cttcaatcaa gaattattcg
     1021 gaaaccaacc gttcgaacgt gcgctgattc atctgatgcg ttcggcttgg gataactatc
     1081 aattattcat gccagaaaaa ttttatcttt taaatgagga acagacaaat ttatataaag
     1141 aagtccagac aattttttcc agctggtcca gccagttacc ttatgatcta cggttgaatc
     1201 ctaattgcat gcgggctttt gtgatcgaat tgagcggaat tctccgtctg acaaaagaac
     1261 atctgactat ctatatcgtc acaaattcgg atgttcatta tctgatttat cgggaagcct
     1321 tggaagctgt aactactttc gattttcaag ttgcaccaac aatttattca agtatatctg
     1381 atataaagaa gtatgcgcag cagtcctcaa accgggtatt atgtgaacgt acattatata
     1441 caccagatgc ggtccaatat gaaaatatta ttcctatttc gatcaatacg atcgatcgag
     1501 ctatcatttc agctgtgcaa aacaaataag gggctgtgac aaaaatcatg tcacagcctc
     1561 ttatttgttg aagatgccca tttctgtaac aatctcttga tacacgtcag gctcacaacc
     1621 tcacttattt tggacatcta cgggtccgat ctctttattt aaaaaataag aaatgatggt
     1681 tcggataact accaacgccg ctagttggaa aatatcctgc aaggtaggtt tgataatcga
     1741 ttcgatgatc ccagctgaaa caagaatttc caaacttaga agtatatagc ctcccaacat
     1801 ctttttaata gcattgttct cgctgactcg gtcgctttgc acttttgtat ggacaaataa
     1861 atatttcaaa aaatccttga ttgatagaca aacaccccaa accaataaac agatagcaaa
     1921 gatatcaagt cccagtacaa gtttttcgaa atatggaaga agaaactgca tcaactctgt
     1981 cgtcatccta cctctcctct tctttgtttt tctttgtctt ctatttcctg attcaagaaa
     2041 taagaaatga ctgttcgaat gataataata agtgcaagct tcaaaatatc tgtccaagtc
     2101 ggcttgataa ctgactcgat gatatcagca acgactagta cttccaagct taacaaaata
     2161 taacttgcta gcagttttct tgtatctgca ttacgagtat tacgttctgc atagtttttc
     2221 ttgacatgag accaatcaaa aagtttgaat agaacaagaa cgatcccgac aattaagata
     2281 ccgatagaac aaatatttaa aatagcaatg atccattcta gaaattcaat taaatttgag
     2341 cgaaacatat acgctaccta ctttctttta atttgttcct attcttttat tgtaaaagaa
     2401 agttaccttg ttcgtctatt taaaagttgt tctaatgata acggttcgct taaatttttc
     2461 tttttcactc tcccattttt ctccctccat ccataaatcg tggaagtatc caaaacaacg
     2521 tagcaagtta aaataaggct tagtccgtca tcagcttttc aaagaagcac tgtctcggtg
     2581 ctttttttgt acccaaattt ctaaaaatag aaccatacac ctcctcaaat cttttcttct
     2641 tacctaccct atttttattt acaggaaaat cagacacttc ttcttttttg tcaaaatttc
     2701 ctttttcttc tcgtcctatc atttggcatt ctaataaaaa cgaactatcc ttggatatag
     2761 aaagagaaaa caaggaggcg tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt
     2821 taggtttgct tgcccttggc gctgcatatc taggaaaaaa gacaggacta ttcgaagatg
     2881 acagtcattt atatgatgaa ttcgaatcaa tctagcatcc tatttagttg aatcactacg
     2941 gacatacttg gaagacattg gaggaatcac aatgagaaaa agtaaagcaa taatgattgg
     3001 tgccggtatt gccaatatgg cagcggctgt ttatttgatt caagaaggca aatggaaagg
     3061 ggatcagatc accttttatt cattggatga tcacggttca aatgatggtg caccgaccga
     3121 cactgtagtg gatgaatatt ggaataaaaa ccatccatta gaaaaccaaa aaggctatat
     3181 tgctcgaggc ggtcgcatgc ttaattaccg tacctatgta gatttgatgg atctgttaag
     3241 tcgaatacct tctgttactg agccagggat gactgctgaa gaagatacga gagattttga
     3301 tgcaaaacac cgaacctttg acaaagcacg tttgatggaa ggtggcaaag gaatcattga
     3361 tggcggaaaa ctgggattca ataataaaga ccgtattctt ctgactaaac tgattgctat
     3421 gcctgattca gaagaagaaa aactagacaa tatcactatc gaagaatatt tcaaagatga
     3481 tccacacttt tttgaaacaa acttctggtt tatgtgggaa acaacatttg ctttcagaac
     3541 acgtagttct gctcaagaat tgcgtcgcta tatgcatcaa atgatttatg aattcactca
     3601 aatcgaacat ctagtgggcg tcaatcgaac acgttacaat caatacgaaa gtattatgct
     3661 tcctttgatc gagtacttga aagaacaagg atgcaaaatc attttgaacc gccgtgtcac
     3721 tgactgggaa ttcaaagaaa caccgatgca agatgaaatc actgtgactg gtttgaaaat
     3781 taccaataca ctgactcaag aagaagaata cgttgtggtc gatgaagata cagcagttat
     3841 tttcactaat gggtccatta cagattcagc tactttagga gattacgaaa caccagctgt
     3901 agaaaatatg gattatggtg cagcttctag tctctggaaa aaagccagtg aacgtttcta
     3961 caacttggga aatcctgaca aattctttgc tgatcgagat gcaagcgaat gggtcagctt
     4021 taccctgacg acgaaaaacc atttgttgtt gaatgaaatc acccgtatca cgactcaaga
     4081 accaggaaat gccttgaatt catttatttc aaccacaccg atcacgccgt taggccaaaa
     4141 agatgtcaat atgtctattg tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa
     4201 tgaaacagtt atttggggtt atttccttta tccaagacga cgtggcgaat ttgtggataa
     4261 acagtatatt aaaatgaatg gaaaagaaat gctagaagaa ctgattggac aactttcaaa
     4321 agtagatcct ggaccagtga atattcgtga aaaagaaaca gaaattttcg acagcgtaat
     4381 aaataatatc cctgtctata tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag
     4441 cgatcgacca aaagtcattc ctaaacattc gaccaatctg gcctttacag gagaatttgt
     4501 cgagcagcct tatcagatga tctttactga acaaagtgct gtacgttctg gagaaatcgc
     4561 tgctttccat tttgcgggta ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa
     4621 agatattcct actctagctc gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga
     4681 aaaagctgcc ctgcatcaag taataagaac aaccaggcta aaacagcttc ttatgttttt
     4741 gatttggttg gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta
     4801 tgaaaaagct gccctgcatc aagtaataag agcagccaag ctaaaacagc ttcttatgtt
     4861 tttgacttgg ttagacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg
     4921 ttatgaaaaa ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac
     4981 gtttttgcat aaatttgact taataccgag aaactgatcc cttctgtcat aacctctttt
     5041 tatttatgtc tgtttctttt tgaaaacaac tattttttca acgtttactc tcttgccaat
     5101 ccaacatcat ctaacggata caactatttc aatgattacc attacactaa aaaatcggaa
     5161 acacacctct ccgaacacaa atacataaga ctatagaatg tttcatttaa gaaaaaaagc
     5221 gtttcattat caaaaaggta agatctcctt taaaatgaaa ttatctgtag tactaataat
     5281 gcagacaatt ttatgaatct gaaaggagca tctccaatga aagcagtcac ttggcaagga
     5341 aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa tattagaacc aacagatgca
     5401 atcatccgta tcacagcaac agccatttgc ggctccgatc tgcatcttta ccatcatggt
     5461 gaagcagttt tggaaaaagg gtatgttgta ggacacgaac caatgggaat cgttgaagaa
     5521 gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg tggttatccc ttttaatatt
     5581 agttgtggtc attgtcattt ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat
     5641 ccagatcagt tatatggcgg gcttttcgga tttggcaagt tgaacggtaa tcattggggt
     5701 ggtcaagcgg aatatctaag agttccttat gcagactcta cttcatttgt tgtaccagat
     5761 aatgatcttc ctgatgaaaa agttttattt ctatcagata ttttgcccac agcttattgg
     5821 agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg tgattctagg ttctggtcca
     5881 gtcggtctat tcgctcaaaa attcgctgtt atggctggag cagagcgcgt aatcgcagta
     5941 gatccagtac agcaccgtct agataaagcg gctaattaca atggggttga aacttatttc
     6001 ttagaagaca cggcacaagc tggaacagaa ttgtatgaac tggctaaagg tggcgcagat
     6061 gtcattatcg attgtgtagg aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat
     6121 ttagtaagct tacaaagcgg cacaatctct ccattacaga tggcatcgca agctgtcaaa
     6181 aaatttggaa cagtccaagt aactggtgtt tatatgactc cagcttcttc ttacccgctg
     6241 caagaatttt ttatgcggga tatcgctgtc aagcatggac aggcaccaat tatccatcta
     6301 atgccaaaaa tttacgcaat gattgcagaa ggactattcg atcctacaca aatcattact
     6361 cattcgctgc cactatcaga agcagcacaa gcatatgaga tctttgacaa aaaagaagat
     6421 aaaaatataa aagttgtttt gaaaccttga attcagtaaa aaatacaggt gattttcttt
     6481 atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga agatatcgaa
     6541 atacgaggac gtcgcttccg cttctatttc caaaatcgtt acacattgat ctcactggct
     6601 gtcgatttat taaccgggat tttctatatt tttggaagcc ttgcttcctt aactaaaatc
     6661 cccgatcgat atggcaccta tttttatttg gctggtgcta ttttcttgac gattcgtcct
     6721 attctcagaa tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat gaagagaaca
     6781 agcaaaaaaa aagaggcatg agaatttttc aaatctcatg cctctttttt caccctatcg
     6841 tatcactttg atacagacga gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg
     6901 tccactcgta tcgttcataa aacgtggtat gatctgtaat taagtaaagt ttttcgtacc
     6961 caagttgatg catttctttt ctgatttccg ctaatatccg cttggctatc ccctgcttac
     7021 gcttttccgg ttcaacaaac agggcacaaa gatttggtgt cagatcaggg cggttatgga
     7081 agtcgttttc aatgacgccc gctcctccga cgatggtttc tttttcatct aagaccacat
     7141 accattgagg aatactttcc ttattagcga ttccagtacg aatactttcc tcatacacaa
     7201 cagcaggaac tccccatttt gatgaaaacc aattcgcgct ttgttttact aattctgggt
     7261 gttctcttaa tttgatccag ttatatgtaa ccaatgttta aacctccgac taaaataaaa
     7321 cgtttacaaa aattatacaa gtctttatga aaattagcaa tgacaacaat agtgatgtaa
     7381 aaaaaaaaac ggaaaacaat cacttgttct ccgttttttg attaatcaag catctaaagc
     7441 ttcttttctt ctttcttgga tcgtagctac cttgacgaac ggtagataga tcaaaataga
     7501 aagaaccaaa ttgacagcgg ctagtacacc acctgcaata ctttttgttg ccaatacacc
     7561 gccaataatt ggtggagtta cccatggagg cataacggta gctgctggca ctaaacctgt
     7621 agaagtcgca aagtaagcaa cactaactaa gaccattggt gtcaaaataa atggaatgaa
     7681 catgatcggg ttcaacacga taggtaaccc aaacatcgtt ggttcattaa tattgaagac
     7741 acctggcgca atcgacaagt ttgttacaac catatatggt ttgtttttac gtcctactaa
     7801 ataaatagct aacagtaaac ctaaagttgc acctgttcct cctaagttga caaagctgtc
     7861 aaagaaaggt ttgttcacga taaacggtgc gctatgtcca gctgcgattg cattaacgtt
     7921 tgcttcaatg gctggcgcat tgattgtttg catcaaagga tcgaccatat tcgctccgtg
     7981 taaaccaaag aaccataaga atggtgtgat aaatgcaagc agtaaggcag atggataaga
     8041 gtttgcaagt cccataaatg gttcttggac cactgtatag aaagaaccta cgatatcatc
     8101 aacaccgaat gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag tgatcattgc
     8161 tggtagtaat gaagcaaatg cttttgcaac tgccggtggc acaccatcag gcattttgat
     8221 gatcaatttt tcatttccgc ttaaacgtac aaagatttct gttgaaataa tcccaacaat
     8281 aatagcaata aacaaaccgt ttgagctcat acctaatgct ccgcctaaag cgaagaaaga
     8341 gcctaaagaa atcacacctg cggaaacacc gtcttttcgg tagccgttag ctaaatgata
     8401 agcaagcaag aaagcaatca agacagaaag aatggcaaat gtaccgttcc aagcagcagc
     8461 cccgaatcct ttccatgatt caccaccaaa aattgagttc atcaattctt ggaacccggg
     8521 aatcggtaag ttattgatca ttactgctaa tgctcctaag atcattaacg gcatactcac
     8581 cataaatgag tcccgaatcg caactaaatg ccgttgtgca ccaatttttg atgctacagg
     8641 tataaaatgt ttctccataa atgcaacaaa tttatccata aatcttccct cttttcttta
     8701 ctgtagatta cgcgttcaat cgacgataca gatcaatgat ctcttttgct aaatctgtaa
     8761 aagctataga tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt
     8821 gatttccttg tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct tggactaaag
     8881 attcctctgc atctttgatt ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag
     8941 caatcgcttc cattgcatcg ctttttgcat ttccgccaaa catgatcaga cccatgatgg
     9001 cttctagatt tttttgatct tccatttatt gtatcctccg ataattttta ttatttgttt
     9061 attagttttt ctgcttggtc gagtactttt tcaccattca tcatgccata gtcagccata
     9121 ttgattacat ctaaaggaat gcctttaggt gctagctttt cagcaaattg attttgcata
     9181 aagcgtactt gcggtcctaa aagtaaaacg tctacatttt tagattctag ttgattatcc
     9241 gcatctgaag cagatacagc aaagatgtct gcttccattc cttttgcttc tgctgccttt
     9301 tgcatttttg tgactaggag acttgtactc attccagctg aacatacaag catgattgtt
     9361 ttcttttcca ttttaaacac gttcctttct atacttacac ttgtatataa gcaagtagcg
     9421 tgccaacttt aaaagcgctt taaaaagttg atatcatcac atttaaggtc tataccaagt
     9481 tgcatacaca ctaaaaaaga aagcgcacac taaacacaca aattaatttt tattaccttt
     9541 tttcttttaa cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact tttttcaaat
     9601 aaacggtatt caaaagcttg catctgcggt aataagtcca accaagttaa aaacataaga
     9661 tgttgttttt actttgctat tcttattact tgatgcagga cagctttttc acaacctctc
     9721 aataaacggt gttcaatcag ctgaccctgg gtattagctc aaaacgctgg attcacaacc
     9781 tcttttattt caaataacca gtaaatacaa tgaacatcta caaaaaactt ctatttattt
     9841 atagatttcg tcaggcaaat ccaattctct cttatcaatc aaagtctgat accacgaagc
     9901 agattttttc aatgttctgc ttcgttcatc gttcaattcg attcgaacta atccatatcg
     9961 attcttaaat gcattcattg gtgacacaca gtcagtgaat gcccataaca tatagccttc
    10021 gcaatttgcg ccttcttctg ttgctttgat caagtaatgc aaatgctgtc cgataaattc
    10081 gatacggtaa tcatcttgca cgattccttt ctcatccata tactgttctt catattctct
    10141 tcccataccg ttttctgtta tcagccaagg gatattatga taattttctt ttagatacat
    10201 ggctatatca tacatgattt tcggatagat ttcccatccc ctggaattat tcattttctt
    10261 cccaggtaaa tcaaagactt cataatatcg ttcaggatgg aaaggtgtct cttcattcca
    10321 ttgataacga ggggcgcgaa cgcgtttagg ataatattga ttgattccaa ggaaatctac
    10381 tgtatgatgc tgaatcgttt ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt
    10441 ttgacacaac tccaggaact cttctgtgta gtatcctttt accatcggat caaaaaatac
    10501 tcgattataa aacaaatcat acatataagc agctttttgg tcttctggag aattggatcg
    10561 agcatagacc atttcaggat ttaaaataca gccaatcctt ccaccataat ttccgctgcg
    10621 gtaaatctct actgctttag ccgttgctaa aactttatga taattccaca tcatccactt
    10681 ttttgtgttt tgttcatgcg gccagcgaac agcatccaaa tagcaacgcg tttggggaac
    10741 gattggttcg ttgaatgtaa accagttttt taccctatca ccgtaacgat caaaggcaat
    10801 ttgagcatac ttagcataaa aatcaactac ttttcttgaa gaccagccat catatttttc
    10861 caaaagaaaa gctgggactt cataatgttc caaacaaatc atcggttcta cgtctgcttc
    10921 gatcaaagag tcgatcattt cacttatatg ctgagcatat tcttcatcta ctatacagtt
    10981 ctcataatca gtaaaaaaac gagcccaatt gatagaagtc cgataatgtg tcagatggat
    11041 ggatttcatc agatccacat cctgctggta ttgttccata aaattagttg ctacggctgg
    11101 gccataccca ttatgccaaa caaaatgttc atttttatac cagctatcta taaacgaatc
    11161 ctgtccttct ttttttcctt tccaaccttc cgtttgccaa gcagaagatg cagcaccaag
    11221 aataaaattt tttggaatag taattttcat gatttcctcc tgttttttag atcccatcat
    11281 tttccacacg atttgctact tttacaaatg gcagatagat caaaatagaa acaataatac
    11341 aaacaagttg cgtaataact gcaccgatat ttcctgctgt tgccagataa gcattgacga
    11401 tcggcggcat tggccaaggg accattacaa ctgctttcgc cgcaaatcct aaagcggttg
    11461 caaaataacc aatcgttcca gtgataagag gcgttaagat aaacggaata gctaaaattg
    11521 gattcagcat gatcggcata ccaaaaataa ctggttcatt gatgttgaag atcccaggag
    11581 caagggataa cttggtgatt tgtttcatat cttcccgttt tccaactaac aatactgcaa
    11641 tcagtaaacc gatcgtgact ccagacccgc ccatactcat atacatatcc caaaatggca
    11701 tagtaataat gttcggtact tcttttccag cttcaaaagc ttccgtattt acagcaattg
    11761 ccgctaaaag caacggttcc cgtatcggct tcaccatttg atttccatgg ataccaatca
    11821 cccaaaagac ttgcgcaata aacatcaata gcaaaatacc cggtaaccct tgtacgacac
    11881 cttctaatgg gcgttgtacg acattgtaaa tgatatcata aaggtacatt cctgtaagtt
    11941 ctttgatgat aaatcccgca gccgcgatac ccgatacagt cagaatcgtc gggaataaag
    12001 cagaaaaact agcagaaaca ttcgttggca cggtatcagg cattctcagc tgtagtttct
    12061 tctgtttcct caaccagcaa taaagttcaa tagaaaggat ggagaccaac attcccaaaa
    12121 ataacccttt cgtatctgta tattgtttag ccaatacgtt ttctactata acagtctgat
    12181 tttcattcac taattcataa acagttggat taacagataa ataagacatc actgcgatga
    12241 taccagggaa tacaccatcc actttattca gcttggctaa ttcgatccct atcagaaaaa
    12301 cagcatagat ggtcaagaaa cttagtgtga catagctgat ggcagatgcg ataggcttta
    12361 attcttctaa aaaacgcaag aaacttatct gtgctaagcc attttccgag tcaaagacca
    12421 ttgctgaaaa caatgtgcca aaagcacccg taataattac cggaatcaat gcagcaaatg
    12481 cgttttttat caccatgata taccttaaat tattgattct cgttgccacg ttccctaaaa
    12541 cattggctaa tttatccata aaactcattc cagatcccct cattcctgct tcttctcctt
    12601 gacgtcatct ctttttgtct attagtcagc gcttacattt cgatgaacag aaactattta
    12661 ctcctctttt ttaccaaata taaaacaaaa attcatctga tgtatttatc ataaaataca
    12721 tcagatgaac tgtcaattag tttctttttc tcctattatt ttctgtaatt ctctccgatt
    12781 tattgccata tggacagtca ttgcatcaaa cgcagcaagt gcattccgcc tttcgatggc
    12841 ctgaacgatc tctgtatgaa tatcgatcgt ctcgattttc agatgctgga tatcataatt
    12901 tttattaaac aaagcaattg attcattaat gattgggata atatgcagca aggctacgtt
    12961 tccgctagct tctgcaatca tcgtatgaaa atcgatgtct aattgaacat gttcttgtgt
    13021 gccttctcga aaagaagttt caatcttttg cgttaatttt ttcatttgga cgatttgatt
    13081 cttcgttgca tattccgctg ccaaacctgc catcttaggc tccaataaat atctcagttc
    13141 aaataagtct cgtatcatcc gatcctgatc ttcaatcaaa gaaaaaccga gtgggtcatt
    13201 ggcaatccct cgcttatcac tgacaaatgt ccccgctcct tgccgaactt caaggatatt
    13261 cctggaagct agagcccgca cagcttctcg caccgtgctt cgccccacct ctaaataatc
    13321 ggacaattca tattcattcg gcagtttatc tcctacttgc aactgcattt cctttaaata
    13381 tctaaggatc ttgtcagtag ttacttctac tagggtttta ttttcttcca aaccacatac
    13441 ctccttttca tctgatgtat tttttcgtaa ttattataac acgtttgaat catctgatga
    13501 ttttttgtat acaaaattga ttaatccgtt catagacata caaacgaaga agatatttac
    13561 cataaaaaag actaggataa aattcactcc tagccttcga tcatttttat tttgaacgac
    13621 aaaaaatatg atgttcaata acagacgatc ttcattcgat tcttgatgtc atcttttgtt
    13681 ttttttaata tatggataaa aagtttgatt acatcgtttc tattctagaa tttggtcgat
    13741 tctcaattct tcttctggtg agaaatctaa attatccatc attcgaacac tttcttgtaa
    13801 ctgacttaac cgacttgctc cgaccagtac acttgtaaca gatttttgtc gcaaattcca
    13861 tgcaagggcc atttgcgcta atgattgtcc tcgtgatacg gcgattgttt gcagtgcttt
    13921 gattttttcc agagttgatc caacttgttc ttctgaaagg aatggtattt ccttacgatg
    13981 tgctctagaa tcttcaggaa taccatgtag ataacgattt gtcagcaacc cttgagcaag
    14041 cggactgaac gtgattgccc ctagcccttc ttcttctaat actggagata gatcgtcttc
    14101 tagtagtgca cgagaaaaca tattgtagcg catctgatga ataatgaacg gtgcttcctt
    14161 tctttttaaa atttcagtca ttttttttgt atcttctcca ttatagttgg atataccaat
    14221 atataatgct tttccttgtc ttacaagttg catcaaagct tctgctgttt cttccaaagg
    14281 tgtatcaggg tctgggcgat gatgatagaa gatgtcaaca taatctagcc ccattctttg
    14341 gagactttgg tcacaacttg aaatgatgga tttacgagag ccccattccc catatggccc
    14401 ttcccacata tgataacctg ctttgctaga aataatcagt tcatcacggt aggcttgaaa
    14461 atcttctcta aaaatacgcc caaagttttt ctctgcagct ccagcagggc ctccataatt
    14521 attcgcaagg tcgaaatggg taattcccat gtcgaatgcg cctcttaaaa tctctctttg
    14581 attatggata ggatcataat caccaaaatt ttgccataat ccgagagata aaatcggtaa
    14641 tttcaaacct gaatgaccgg atttacgata ttccattttt tcataacgtg cactatctgc
    14701 aatatacaat ttttctcctc ctcatcaaat ctttttaaaa cgctttcttt tttctatttt
    14761 tagtttacta cattttaaag aaaaggagtt aatcaaaatg attttttcgt atattttttt
    14821 gccatttgtt ttttactcgt tgatagatca gaaattgatt acgtgcttgc tggatttttt
    14881 tataacgttt cggttgtttt ttgtaaaatt gctgatgata gttttctgct ggccaaaacg
    14941 taccggctgg ttcgattttt gtgacgattg gttggtggaa agttcctgaa ctgtctagtt
    15001 tctgttttga ttgttccgct aattcttttt gtcgttcgac ttgataaaaa atcactggac
    15061 gatattgctt ccctctatct tgaaattgtc caaatgtatc tgtgggatca gtaatttgcc
    15121 aatagattgt cagcaactcg ctataactta tttctcttgt gtcataaatg atctccacag
    15181 cttccacatg acctgtgtaa ccaccactta cttgatcata atttggtttt tcaacatgtc
    15241 cgcccgtgta tcctgacaaa acagatagga tccctttttt cgattcaaac ggctcgacca
    15301 tacaccagaa acatccccct gcaaatactg cacgttcttg atagtcagca tcttgcatgg
    15361 catgcttgga aaagtcatat ttttgctcct tatcaaatcc ttccgtaatc aataaataaa
    15421 agtccattac atcaggcgtc agattcattc ggatagctaa tggacgtagt gttgcttcta
    15481 gttttgataa ctgttcttta acggatatat tttctcttat gtcattcttt gtctgaatca
    15541 atacttttcg ttcccaatct cgtgtattcg gattcaaaat cagattataa agtgtttgaa
    15601 tgaatatttg ttcctcatcc atagattctc ctcctttccg aagaagacaa gctagtgact
    15661 ttagctgtaa tttatcataa agtgaggaaa taagcgatta cttcatcccc ctacagattt
    15721 ttcgtataaa aaagagagaa tccttatcga cagattctcc tttttaatca atttatttta
    15781 taaatgaaaa gttaatttac cttttatgga aaactatttt aaaaaataaa cataaatatg
    15841 cagttaatgt aacaagataa gaaataatag tcgttactgt ataacttgtg cttactttaa
    15901 tatcagcaac tcctttgact ccgcttattt gtaccaatcc attagcattc acctgctcct
    15961 ttacaggttg atgatttatt tctacattcg ttcctaaata agcatataca ggaagtgtga
    16021 ttacttggct cttctcatta gttactctat atatgataga actaccagtt gttttataag
    16081 gaaggtttac ccattgactg ccaatctttg cttcgtgttt tattatatgc tccttattct
    16141 ccaatgaagc gataggagaa taatcatata aaaaataatc cattccttta gtggtatacg
    16201 cctctactgt ttctttgttt acaacaatat gtcccggctg agaaattatt ttttgattga
    16261 aatttt
//
LOCUS       DACCXM010000059        16249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCXM010000059 DACCXM010000000
VERSION     DACCXM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.061"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..173)
                     /locus_tag="IVY61_001646"
     CDS             complement(<1..173)
                     /locus_tag="IVY61_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7970761.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IVY61_001647"
     CDS             complement(593..1714)
                     /locus_tag="IVY61_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP7970762.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IVY61_001648"
     CDS             2042..3451
                     /locus_tag="IVY61_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP7970763.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IVY61_001649"
     CDS             3448..4176
                     /locus_tag="IVY61_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7970764.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IVY61_001650"
     CDS             complement(4304..5215)
                     /locus_tag="IVY61_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7970765.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IVY61_001651"
     CDS             complement(5232..6239)
                     /locus_tag="IVY61_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7970766.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IVY61_001652"
     CDS             complement(6236..8365)
                     /locus_tag="IVY61_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7970767.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8495)
                     /locus_tag="IVY61_001653"
                     /pseudo
     CDS             complement(8370..8495)
                     /locus_tag="IVY61_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8666..9853)
                     /locus_tag="IVY61_001654"
     CDS             complement(8666..9853)
                     /locus_tag="IVY61_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP7970768.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(9946..12291)
                     /locus_tag="IVY61_001655"
     CDS             complement(9946..12291)
                     /locus_tag="IVY61_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7970769.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
     gene            complement(12278..12667)
                     /locus_tag="IVY61_001656"
     CDS             complement(12278..12667)
                     /locus_tag="IVY61_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7970770.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            complement(12717..13613)
                     /locus_tag="IVY61_001657"
     CDS             complement(12717..13613)
                     /locus_tag="IVY61_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7970771.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            complement(13616..15463)
                     /locus_tag="IVY61_001658"
     CDS             complement(13616..15463)
                     /locus_tag="IVY61_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP7970772.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            complement(15560..16060)
                     /locus_tag="IVY61_001659"
     CDS             complement(15560..16060)
                     /locus_tag="IVY61_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7970773.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            complement(16073..>16249)
                     /locus_tag="IVY61_001660"
     CDS             complement(16073..>16249)
                     /locus_tag="IVY61_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970774.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
BASE COUNT         4915 a         3396 c         2675 g         5263 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttttcagtca agtccacaat cttgtgtaaa
     8521 agttcaacct catcaaaagc acaatgtttt gcgttgtgaa cctaactgcc atgcctttct
     8581 ttctgaggag agaggtcagg agcagtagcg cagcgactct tgatgtctct cctcagaaag
     8641 aagagactcg cacaggttca tggtgctaaa tgttattcga attaaattct aaatattttc
     8701 gtggagaagc cagccactca gtgtcttttt ccatcaaaag tgttccaatt atccgagtga
     8761 tggaattgat atttggaaaa atccgaatca ctcgttctct ccggcgaatc tcttcgttca
     8821 accgttccaa cacattggtt gtacgaatcc ttcggcggat attttcagga aaagacaaaa
     8881 tttgaatagc atcttcaaag ccgttttcta aaatgtcgca agcagcactt aattttgaat
     8941 cacaaccata tttttccaag aagtgctctt ttctttcacg tgtcaattca agttcagagg
     9001 ctttaaagat actcttcaac tcgtctttta catcagaaga aactttcttt ggcagcttat
     9061 caaaaatatt tctcaaaaag tgggcttggc atctttgcca gctcgcacct aaaaagttct
     9121 tgcgaacagc cttaactaac cctttgtgtg catcagaaat gaccagtttt acaccgaaca
     9181 aacctcgttg aacgaacgat tgaaaaacgg tatcccaatt atcttccgat tcgccgtctt
     9241 gaacatcaaa accaagaatc gtacgtcgtc catcagaatc aatgccgata ccaatatata
     9301 caccttttga gacaatacga tgattttccc ttactttcat atacagggca tcacacatga
     9361 aaaatggata ttccgagcct tctaggctcc ggtttctcca ctcaaaaaca aatggatcca
     9421 actgtttcat cagatttgaa acgaatgatt tagaaacagt cgcaccatcc gttagcagtt
     9481 caactgtttt agttactttg cgagtggaga caccagaaat gaccatttca atcatggcag
     9541 cgatcaaagc tttttcgctt cgctgatatc tttcaaagat agtaggacta aacttaccgt
     9601 ctctggttcg tggaacatga agttctaacg taccaattcg agttgtaaac gaacgatcgt
     9661 agtaaccatt tctttgtgat tgccgttctt ctgaacgttc ataggcattt gcctgaatat
     9721 attgatttcg ttgttcttcc atcagctgat taaacatgat agtcataagt gtttttgcgg
     9781 tatcattcgc accgctttcg tttataattg cttgaatttc ttcgctttca aaagtaaaat
     9841 gtacttgagt cattgatatc agtcctattc gagtatgttt tctgcaaaaa acattgtact
     9901 cctaactgag atcaatggct tttttgatgc ttcactttta cacaattata tggacgtaat
     9961 cccgttttcc aaatctcgca ggcgtttttg attgctttga tcctcctgat ccaaaccaaa
    10021 gaattccaag gttttcttga tttcatgaat atcggtactg cgaaaagcaa acttcaaccc
    10081 gatattattt tttaaacgtt catctaataa atcgtcgcta ttttgagtga cgaaatagac
    10141 cccagcattc atggcccgac cagcacgaac aagcttattc gataacgttt ttccttgagc
    10201 cacttgaaga aaactccaag cctcatccaa atcgacaatt ttgaagattt ctcggttact
    10261 atggataaag tccaaggcaa aagtggaaat gacgattagc atcgaaacac taagcaattc
    10321 catggtggta tattcttcaa aagtcgtttc tgcatccggt aaaaccaaat ccgccacttg
    10381 aatgatattt agctgtttat ctaaactaat cgattgggta atcgagccgt ctgagaaaag
    10441 caaatgagca aaatcataat cggtaaagga ctcgatatga tcggcgatat tttccgcgat
    10501 tggtgacccg tcttttcgca attcgttaat aacttgtaat aacccccgtt gatcactttg
    10561 agtcaccgtt cgaatggctt tacgtaacac aggaaacttc tccccgtctc gacttgaaat
    10621 tcctgttaaa aaagtcagaa tatcaattgc taaactttcc gagtctttct ttttcttcat
    10681 aatcacataa ggatcaagca gaccttgatt ttctgactta ctcgtcaagt tgaggatact
    10741 gatttcatcc gcaatttctg gcaaggtttc tttccaccct cctcgctccc cttttggatc
    10801 gacaatcaca gcttgaccac cgaacaaaac agcatagtag actaagagat tgttactaaa
    10861 ggatttacct ccaccaagag aacccaggaa agcggcggcc agcgcattgg ttactgaacc
    10921 cttgactcct tgtgaagcca atgccggttt caggtaaaca ttacgacctg tatccacatt
    10981 gtagccaaag taaatccctt ctaattctcc tagcatctga gtagccccaa aaccaagccc
    11041 cgccaaaaaa tcagacgtaa catactgaat atagtcgttc atatatcgtt tacttgaggg
    11101 taagaattct tcatgtagcc ccatcatatc gccaaagggc cggactagtt ttacattggt
    11161 atcatcataa aaatcgagca cttcatcaca gcgtcgcttt agttcatcca ctgattcggc
    11221 actcactcga accacataac tcagtttgta catagattct tttgactgat ccaaggtggt
    11281 ttccaattca tccacggaat ccaaagcatc taagacattg gaattcgttt cattgtcaga
    11341 ttggtaagca tgattgtcta aatctttcag ttcttttttc ttattacgaa cggtagctaa
    11401 agccttttta ttcgtcacaa tctctacatt catacttgtg tcgattggga atgtaaattg
    11461 ttgttgctgg taatagaaaa gttctgagga aggaaattcc atctccccca caattgtatt
    11521 aattgttaaa tacgctacgt acgactcatg attctcatgt tccatccgta agcaccttgg
    11581 tttttcttca attaaacaac gatttggacg taataagtca taacgtttca ccagtgtttc
    11641 tgattttagc tttttctttg gtagctgaaa ttcgtattct tcaaacggta tccctttttc
    11701 gccataaata tgttcaatca aatacgtcag gtcactcggt gtgactcgcc tgactttaaa
    11761 tcttcgagcc aatttgcttt ccattaattt ttcaagctta gaatagcagc gaatttcttc
    11821 attggataag gaaacaaaat cgcccatcaa atgatgattc actccataga caaattcttg
    11881 aagcccagaa aagaaagatt tcttgagact ctttagattg acttcttcat ctgtcgcaat
    11941 tagtttaaag ccaataaaaa agcgataatc aatctgacta tcgccaatca tggatactaa
    12001 tgcatctgtc tgtatatcaa ttcgttgttt cgctacttct ttgagtctac cggtaatttc
    12061 tttttttgat cgttcttgtg tggcgcgaat actactttct gtggcaattt gtaacgcatg
    12121 gatcttgcct tcacgatttt gcgcaattag ttggcgaaag ttatcatgca cttgaaattt
    12181 ctgttcgggt gacaaaaagg agtaattgta aggcactaat tcatagtagg caaaacactc
    12241 gccttcttgg ttaaagacga gattattttc aatatactta atcggataca tagttcacac
    12301 tccttacaag agtactgggt tcatcccatc gtttcttctg ttctttgact ttctttcctg
    12361 caaaagtcac cttaaatcgc aagtgatagc tgataaagga tcgtaaaaat cccagtggtt
    12421 tcttgccatc aaacgtcttt tgcgacataa accaagtgac agccaccggt atgcctaagt
    12481 attttaaaaa gacctcctct atcatagata gtggaggcac atgggcaaat acgatgacaa
    12541 acagtaaaga taaaacgaac caagccattt ggttaaaagt cacaggaaaa ggcaactgaa
    12601 aatcattgat tgcgtaaatg actttttcaa ctgcccagat actggtatag ctttttattt
    12661 ttttcatttt tcctcctaaa aagggcggac ataataatat gtctcaccct ctagtgttat
    12721 tctacaattt gtttaaaacc atagcgtttc gctctatttg taaaagcatc ctcgccacct
    12781 tccatcacag tacatattct tggcttgata ttgggttcat ctaccgaccc gcactgatca
    12841 gccgatattt cttttgaatc tcccatgcta atcaaccatt ctgaatcacc attaactggg
    12901 atatttgcat tatgagttac aattaaaatt tgtctgtttt ccttttttgt aatgatactt
    12961 cttacgatta agttattaat taattgacta tccaaatcat cttcaggctg atccaatatg
    13021 agtggtgaat ttccatacgc caaaatcata gaaagaattg cagatgtctt ttgaccagca
    13081 ctagcattcg ataaactaac tttttttcca tttagttcaa gtcggataac tactaaatca
    13141 tttggcttaa gggtttgtaa ttcaagaact tgtgcatcat tcaattttct tatagaattg
    13201 attaattttt tatctgaaaa aatactactt tccccactaa ctcttatatc aagtatgtca
    13261 ttatatatat cttgattagt aatggaattt tttgtcagct tatcaataag agatccaaac
    13321 tcactgtcaa aagtgtcctc cttattaata caagctctga gctctttaac ataattattg
    13381 aaatccctat ttggttgaat agtagcctct attcctgtaa tatcagaaaa aatttcgcgc
    13441 gaggaactac ttctctcaga atagagattt tgatatgatt tattcattct tgacattgct
    13501 gagtcaacct caacttggta atctttaagc tttatttctt gttgaataat ttcttccaat
    13561 cgctcttcag tttttgataa agattgatat aaagaatcaa ggttttgtaa catttttaat
    13621 tcatcttgag ataactcttc actaagtttt tgatacctct ttgatacctc tttgaaacct
    13681 cattatagtt aactttccaa tctgatttat tcagctctgc tctataacta tcaatttcat
    13741 ctgacatttc attaattatt gtttgaatct tagcccgatt ggtcactaaa ttcttaatca
    13801 attctccgcg gtgttcatca atttcgtctg taacttttat accattgcca aagtcaattt
    13861 tgtcaacact atcagttaga tttttgggaa tttccttaag tgcctccaca tcacgtttca
    13921 gttccctata aatatctgtc tcttttgaat actcatcata aacatcttta taactcttat
    13981 gagatagctt ttctacttgt ttttttaaat cttcaatttc aagctctacc ttcgccttat
    14041 cttgtatttc ttttttcaca gaaacaatat cccaatttaa ttttcttatt tcgtttacac
    14101 atgtttcaat ctcattttta tactctgaaa tcaaatcaga gttatatcca tctagtaatt
    14161 ctagtatact agtttgattt tttgaaattt cataaatttg tttttgagag tatatactca
    14221 ccttatcgga aatcattctc aaccgttctg tttctttttc aaccagacca ttctcttgtg
    14281 taaattcata aatagaatgt ctatcctgtg ttcttattat ttgatatttt gtccccttat
    14341 agaaaatatt cagtttcaca attgtatcct tcaaaaatat tccagagcca ttatgttcaa
    14401 tttgtgcaaa gttctgataa tcactttgta tttcagaaaa ttgatcaagt tctgcttcct
    14461 ttccaaggac atagaccaag aatcttgtaa tacaagattt gcctgttcct cttcctccaa
    14521 taattgaagt taaattatcg ctgaattcga ctacaacttc ttttgtgcta agagtggtcc
    14581 cagaaaagct taatttttcc aataaaactt cattttcatt aatggagaaa ttagaaaaat
    14641 cagaatgaac tcgagtttcg cctagcatta aagcatcgcg aagtgatccc aagtcaggtt
    14701 cgtccttcat ttttatatag gaatatcttg tgcctattcc ccaaagtcca tgttttgaat
    14761 ttcctttcga gtgtgggtta tcagaaaagc taagaaatgt catattattt ttttctgcct
    14821 caacaactga tttataccaa gattccaaag tatcttttcc aactctacca tcatatctgg
    14881 cattcacact atcatagata gtgtcacgct tagatgaggg aatcatttgt gagcgatttt
    14941 caaaaaattc tttttgaacc gtctgtactg catatatatt ttctgattca aatatctttc
    15001 ctagatttcc aaattttgct gaagaaattc cattaaactc atctatatgt gcaggaataa
    15061 cgagcaagcc ctcgtgctca gcaaaatcaa taacttctaa aactgatttc attgagcaag
    15121 ctgcagcgct ggcccaatcc tctcttttta tacccatctt ggttaagaaa tcagagatat
    15181 catttgtaca tgcactttta tcaaatagga ctagtaaatg agttccattt tctccacacg
    15241 ttaattccac tccaggaaaa aaatctaagc cattttcgat agccactttc ttatattcat
    15301 caatattttc ccctgtattg tgatcagtta atgcaacaca atcgagtcct ttgtttaaac
    15361 attcttctac ccattcctta gcagtaacct ttttatcctg aaagcactga ctcgcagttg
    15421 aatgaatatg aagatcacat ttgtaccact tattgccaac cattagtaac acccctatca
    15481 acaaatattt tctatttcag tatatcaaat agaaaagtaa aagtacgata gaaatttctg
    15541 gtcaattcta acaaatgatt tacattagtt caaaaacccc tcgactcgta accaaataat
    15601 tcccgccaat ctccaagtct cgaccacacg catcaaaatc aatatacgtc ttcaagtgtt
    15661 ctggtacttc gcctaaatca ccagcttcta caatcaagta atacgctaca tcactcatgt
    15721 cattacaatc gggataatag atgatatcat ccacatgctc taccatttcc tcgaaagaat
    15781 tgaagaaggc atgttgaatc tctgaagcga cctcaccaat tggcgttcct ttaagctctt
    15841 ccgctaaacc acacaaccga ttaatttcct caattggtgt atactcatca atctcaaaag
    15901 gcagttcata atcatgaatt gcgtactctt catattcgtc atttaatcca atccgttctt
    15961 tcacttcatc aaaatcgacg ggtggggtaa accatgcccc caccaactca ccttcattgt
    16021 attttccaag gttcgcaata taaacacgta tctgttccat tgtgattccc cctcaggcac
    16081 cgacaattcg gttaaacaaa ttgagtaaga catccttcac accagaagca ttaaagacca
    16141 gacctaccgc aatcaaagca atcaccaaga agccaatcag tttggaaaac tcgcgtttaa
    16201 atcctaaaaa caaaccaatg accacaatcg ctagtaagac taaagattg
//
LOCUS       DACCXM010000060        15441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCXM010000060 DACCXM010000000
VERSION     DACCXM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.062"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..213
                     /locus_tag="IVY61_001661"
     CDS             <1..213
                     /locus_tag="IVY61_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970775.1"
                     /translation="GEVAASLARKDEHGLRDGIGDVVVTLIILAMQNDMDLYECLNQA
                     YNEIKGRTGKMVDGVFVKSSDLEDSK"
     gene            210..428
                     /locus_tag="IVY61_001662"
     CDS             210..428
                     /locus_tag="IVY61_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HAP7970776.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            441..722
                     /locus_tag="IVY61_001663"
     CDS             441..722
                     /locus_tag="IVY61_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970777.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            719..1045
                     /locus_tag="IVY61_001664"
     CDS             719..1045
                     /locus_tag="IVY61_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970778.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            1042..1239
                     /locus_tag="IVY61_001665"
     CDS             1042..1239
                     /locus_tag="IVY61_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970779.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            1236..1445
                     /locus_tag="IVY61_001666"
     CDS             1236..1445
                     /locus_tag="IVY61_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970780.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            1442..1729
                     /locus_tag="IVY61_001667"
     CDS             1442..1729
                     /locus_tag="IVY61_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970781.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            2321..2788
                     /locus_tag="IVY61_001668"
     CDS             2321..2788
                     /locus_tag="IVY61_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP7970782.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            3087..3428
                     /locus_tag="IVY61_001669"
     CDS             3087..3428
                     /locus_tag="IVY61_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970783.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(3421..3600)
                     /locus_tag="IVY61_001670"
     CDS             complement(3421..3600)
                     /locus_tag="IVY61_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP7970784.1"
                     /translation="MYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDVTK"
     gene            3962..4174
                     /locus_tag="IVY61_001671"
     CDS             3962..4174
                     /locus_tag="IVY61_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970785.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            4181..4405
                     /locus_tag="IVY61_001672"
     CDS             4181..4405
                     /locus_tag="IVY61_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970786.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            4432..4965
                     /locus_tag="IVY61_001673"
     CDS             4432..4965
                     /locus_tag="IVY61_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAP7970787.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            4952..6196
                     /locus_tag="IVY61_001674"
     CDS             4952..6196
                     /locus_tag="IVY61_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAP7970788.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            6209..7708
                     /locus_tag="IVY61_001675"
     CDS             6209..7708
                     /locus_tag="IVY61_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7970789.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            7713..8639
                     /locus_tag="IVY61_001676"
     CDS             7713..8639
                     /locus_tag="IVY61_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP7970790.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            8766..9368
                     /locus_tag="IVY61_001677"
     CDS             8766..9368
                     /locus_tag="IVY61_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP7970791.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            9381..10304
                     /locus_tag="IVY61_001678"
     CDS             9381..10304
                     /locus_tag="IVY61_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7970792.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            10322..10585
                     /locus_tag="IVY61_001679"
     CDS             10322..10585
                     /locus_tag="IVY61_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970793.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            10597..10926
                     /locus_tag="IVY61_001680"
     CDS             10597..10926
                     /locus_tag="IVY61_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAP7970794.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            10923..11249
                     /locus_tag="IVY61_001681"
     CDS             10923..11249
                     /locus_tag="IVY61_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970795.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            11233..11571
                     /locus_tag="IVY61_001682"
     CDS             11233..11571
                     /locus_tag="IVY61_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP7970796.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            11571..11963
                     /locus_tag="IVY61_001683"
     CDS             11571..11963
                     /locus_tag="IVY61_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAP7970797.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            11974..12486
                     /locus_tag="IVY61_001684"
     CDS             11974..12486
                     /locus_tag="IVY61_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAP7970798.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            12532..12900
                     /locus_tag="IVY61_001685"
     CDS             12532..12900
                     /locus_tag="IVY61_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970799.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            12954..13241
                     /locus_tag="IVY61_001686"
     CDS             12954..13241
                     /locus_tag="IVY61_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970800.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            13258..>15441
                     /locus_tag="IVY61_001687"
     CDS             13258..>15441
                     /locus_tag="IVY61_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7970801.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMI"
BASE COUNT         5528 a         2365 c         3288 g         4260 t
ORIGIN      
        1 ggggaagttg ccgcttctct agctagaaaa gatgaacatg gtttaagaga cggaattgga
       61 gatgtagtag taaccttgat tattttagct atgcaaaatg atatggattt gtacgagtgt
      121 ctgaaccaag catacaacga aatcaaagga cgcacaggaa aaatggtaga tggtgtattc
      181 gtgaagtcga gtgatttgga ggacagcaaa tgatcagcct aacaatattt tggtgtgtaa
      241 ttggcggctt actagcgatg ttaggagcat acctcattta cgagagtgca aaaggcaaaa
      301 aatatacagc agtagggatt attttgtttg ttgtcggaat tttagttgcg attggtgcag
      361 aaataaatct ttatttaaac ggtacgacag aagagttgat gtggttttta ttgtggacaa
      421 ggagatagga ggacagcgaa atgggcgata tgggcgaata ctggaaagat attaaaccgc
      481 atctaaaaga acgtagaaaa caacatgtca gaaagatggg caattcagca actaaaaata
      541 ttgaaacctt gggatttgaa tttactcatt atccaaacaa tcatcaattt gcaattaata
      601 cccataatgg aattattgac tactggggaa ctacaggaac gtggattgag cgcaaaacaa
      661 aaaaacgtgg taaagggata cgtagtttga ggaaatatat taatcaggag gacagcaaat
      721 gaactataca caacaagaac tgactgattt atgtccaaaa catgtagctg agttcatcaa
      781 taatgaagtc ctgcctaaat atgcagatgg tttaaataca gcagaaaacg ttacagattt
      841 tatgattaat gatgctattg atcgtttgat atttttagaa atagattgta ttgcttatta
      901 tcgtctacac gcagaagttg ctttgattga tccgtatatt gctttaagcc aaaatcgaaa
      961 aatactcgta gcctatattg ataccaaaca gattttagaa ggcagagatg tcgagataga
     1021 gatcgatgag gaggaagtgg gatgaaagca tacacaatca agttttatgg aaataaaacc
     1081 tatgtataca tcctaagcgg tctcagactt gattttagag gctattcaaa agaagacctt
     1141 ttagaatcct tatatggtta cggatattta aatgaagatg aactaataag cttagagaaa
     1201 tttacaaatg cttggacgat tgaggaggaa gcagaatgaa gatttatgta gtaaagttcg
     1261 ggaataaatt ttatagaagt gatgaacgct ctataggggc taacacatta ttcattgtag
     1321 acatactcca aagtgcgaga tggtttgata gcctcgaaga agctaaccaa gtttcacgac
     1381 gacttggtgg attaacacaa gtatatgaac tggtcactgt cgatcatgag gaggtagaaa
     1441 gatgaaatta aaagacggat tttacgctag tagtcatggt atcggcggtt taatgctaga
     1501 tatgccgaca aagaatccta aaaaacgtaa gaaaccaaaa ttcaaagtcg gtgacatggt
     1561 tcgctgcgaa gcagaagggt tcatctatcc atttcgtgga tatgtagaac acgtctataa
     1621 tcactcagcg atcattcgta ttgaaaacac gatggaatgt gataagtggt tagcgaaaag
     1681 caaagagaat ttagctgtag cgagattggt ggatatgaag gctatataaa cagcgaaaaa
     1741 atgtacttct aagagaaaaa tttatttttt ataaaaatag gaaagtatca ttatgaaaaa
     1801 gaccacagag ataaataaac ctaccacata aaaaatgaat atttcttgcg attcaatata
     1861 ggtatattta acaatttgta caataaaaaa tactataggt aaaaaagata acatgaggag
     1921 cttatttgtt ctagagtgtt tgacaaagta acaaaatgcg acgataccaa tacaaacggg
     1981 aatccaaaat aacagcatgc tccacatagt cactcaacca tctcctatac gctttattat
     2041 caatagataa tatcattttt cgtaataaag ataaaaaaat aagtaaggct tctcattaaa
     2101 atataaaaaa gacagccgac cactggctgt ccttagcaga atattgaaat agtgagccat
     2161 ctgttttccg ccagatagct caacatgtat atgggtaacg agtgcaacct cgctacttga
     2221 aaagcttagc ataagcatgg gtataaaaaa agccggattc ctccgacctt aattaataat
     2281 tctgacacaa ttattatatc ataaatggag gaatcaatgg atggtacttt tcgatgtaaa
     2341 gaagtatgag acaccagagg caaaagatgt ggatatggat cgcacaaaac ataatgttgg
     2401 tgtttttctt tcagcatatc tgtcagctag atgtaggata ggtcaacctc gggagcctaa
     2461 agtaacggca tcttactcct tggttccacc ttctacagca aataatacat ttgaagcaga
     2521 acgaatgatg attgagagag aggaagcgca agaagaattt gagtacttgc acaaattatt
     2581 tgttcgtggc tattcagcaa ttcagcatcc gcataaacct gatgtaacag aaaggcgcaa
     2641 gaagatattc tatgatcgtt atatcaatgg tctgcccatt tatgtaactg ctcaaaggaa
     2701 taatactagc gaagaatcgg ttaaagtaga atcaaacaga attatcatcc aatttgcttc
     2761 atcgttagaa ctggttgctt ttaagtagcc agtttttaca ctttttatac cctttgatta
     2821 cccatttggt ttccatttta tacctttttt ataccaatca cttacctggt aaatgattta
     2881 ttatgatagt gtcgaaagat tagtgatagg tctgagacaa aataataata aaaggaacat
     2941 cgttttatta ttgtttcaca attaagcttc gatagacagc aacggaaata ttaagaataa
     3001 ggatgtgaat tccaactcct tctaaattgt tcttattatc tatcatccgt tgctgtttat
     3061 taatttatgt attggaggga aaacgaatgg aagtcgagca aataaagtat caaggtatgg
     3121 atcgggaacg gttgattgat attattgagc aacaacgttt agaattgatc caaaaagaag
     3181 ctgtatgtaa agagtacaaa aaacatttag aacaagtaat tgagtatcat tcagtagaaa
     3241 agtacagatc agtggtgcaa aaaaatagag caacgagtcc tgatcctcgt caagatgtat
     3301 cgattagtat caagacgcca gtatttaatt ctgttaaatc agaaaagcta gaaagtatct
     3361 caactgcttt ggtagaaaaa gcagctgaaa tgaactccct agcaaatgaa gttagtcgtc
     3421 ttatttagtc acatcgataa cttgagagtc tttggtgatt ccgtttttta tagaattaat
     3481 ggtttgttca gcggaatatt tagtcaagta tgtttcgcta gttgctacta cttcattgtt
     3541 atcggactta ataacgaagt aatattggcc attagttgct tctcttataa caaagtacaa
     3601 atttttcacc accttttaat ttatttcagc ggaccactcg ctgataaata aaattataca
     3661 cttagtattt attttcacaa tattaatttg tcactgtggc ggaaagggta gacgctaagc
     3721 atgtgtgtta ggtcaatgct tcggcaacca tgcaaggttc gattccttgc cagtgacttt
     3781 ggcaactgag ggttggaaat gggcgctcaa agtacacgag caaggcgagg tcgatagtaa
     3841 tcgatggaat cggtgtaggt tgcttgatag aactataact gcatctcgtt tatgaggtgt
     3901 agtttttaca tattagatca ctcatcgagt ggtcttttta ttttggaagg ggagtaaaaa
     3961 aatgaacgaa aaccaattaa gagagttgtt taaaacgaat gaagcaaaca aaacaatgga
     4021 ggcgacattc tacgaaactc aaaaaagctt agcgttaatc gcaaaacaag ctaagtattt
     4081 ctacgatcag cttattctgc aaggatttaa tgacgcacag gctatggaat ttatgatgcg
     4141 aaccttttct gccagtaacc aacagaaaga gtgatacgta atgaggaact actggtatgt
     4201 atcattatca aataaatatc cgcaaccaaa cgcagatgat ccaattagag ttgtccagtc
     4261 agtccagatt aaaaagaagt actccatcat tgaaatgacc agagaagcta cgccaaaaga
     4321 gattgataag tacaatcttc gttactgcgg ccatggatat tttagtgagc agaacataca
     4381 gacaaatatt gagagatatt gttaataaaa ttgaaaggtg gtggcttgaa tgtggtaaat
     4441 ttgacaccaa aacaaaaagc ttttgcggat gaatatataa aaaatggtgg taatgctact
     4501 caagccgcca ttaaagcagg ttatagcaaa cgatcagcaa gagtaatagg taaggaaaac
     4561 ctaactaaac ctaacataat acagtatatt aatgaacggc taaatcctat cgaaaagaag
     4621 cgcaaattaa gtgccgagga cgctttaaat gaattgatag atatttggca aggagaagta
     4681 caaataagcg tgagcaagca aatagaccgc ttggagaaaa acaaggttat taaacatatg
     4741 caatatgaat atacaccaga tttagaaagc aaattgaaag ccttggattt gtatttgaag
     4801 tataaatcgc tgttatcaca aacgcaatta gaaaaagctc aaacagaaat aaaattaatg
     4861 caagcaaaat tagaacaatt acagataaac tcagagcgtt ctaccgaaga aaaacttgat
     4921 gagttgttag aaaagattag tggtgaatta gatggcacta gttgatattt ataacccaaa
     4981 gcaaatcgac gtgttaaata aaaccattaa gaatgattgg tttattactt tattacatgg
     5041 agcaaaacgt tctggaaaaa caaaaataaa caatgatttg ttcttgtttg aattgcgacg
     5101 tgtgcgaaag attgccgatg aagaagacat taaggaacca atgtatatcc tagcaggagt
     5161 ttcaagtgca acaatccaaa agaacatctt acaggaacta tacaacatgt acagcataga
     5221 acctaaattc gataaacatg gaaactttaa gctatttggc gttaaggtcg tacaagctta
     5281 tacaggaaac attggcggtg ttggcgcaat tcgtggtatg acagcatatg gcgcttatat
     5341 caatgaggca tcgctagcta gacaagaagt atttgctgaa atcgtttcac gttgttcagg
     5401 aacaggagcg agaatactag ctgatacaaa ccctgacaac ccggaacact ggctaaagaa
     5461 agagtatatc gacaattcaa gcaaaaatat tcaatcgttc cactttgaat tagatgataa
     5521 tacattttta tctgaacgat atcgtaacaa tattaaagaa tcaacaccaa gcggcatgtt
     5581 ttatgatcgt gatatcaaag gtttatgggt ttctgcagaa ggtgtcgttt atcgtgattt
     5641 tgatgctagc aagcactaca tccagtcaaa agacttacca cctttgagca acttttattg
     5701 tggtgttgac tggggatatg aacactgggg ttcaatcgta gttataggtg aaacggatga
     5761 cggaacagct tatttaatcg aagaacatgc tactcaattt gaagagattg attattgggt
     5821 agatgtggca aaaggcattc aagagcgtta cggcttacga gtaccttttt attgtgactc
     5881 tgcgagaccg gagcatgtgg ctaggtttgt aagagaaaag attaatgcta aaaatgctca
     5941 taaagcacgg ttatctggag ttgaggaagt cgccaagaga tttaaacaag ataaattgtt
     6001 tatctgtcaa gatagagtga tgaaatttcg ggatgaaatt tatcaataca tttgggacaa
     6061 gaaaaaaggc gagccaataa aagaatatga tgatgtgcta gactccgttc gatatgcgat
     6121 atatactcat gagcttctta agaaaccaaa agttaatgtc aacgaaaaga ttaaacgtat
     6181 taaacgcatg ttttaaggag tgtgaaaaat ggataaggta aacgagtttg aatacggagc
     6241 tgatatacat tattctaatg acgtgaacac aaattatgta aagtttagcg tagactcaaa
     6301 tcttcactat agatttagct cagcagaaga tttacttaac gatttagata ctttagcagc
     6361 aatgataaaa catcatcatg aatatcaggt aaaaaggcta agtgtattag atgattatta
     6421 taaagctaga aatacaaata tcatggataa ccgtaggcgc agagaaaaag aaaaagcgga
     6481 tcaccgatca gcacataact ttggaaaagt tctttgtacg tttgatgttg ggtacaacac
     6541 aggcaatcct ataaaagtgc aaatcgagga cacaaatcaa caaaaagaaa tcgaagagtt
     6601 taatactaat aatgacatag atgggttaaa tgctgaactc tggcttgata tggataagta
     6661 tgggagagcc tatgagatta tctatcgaga ttcagatgat acagattatg ttgatttggc
     6721 taatgtattt gaaacgtttg ttgtatatga tactacagta aaacgagagc ctattttggc
     6781 tgtacggtat cctaagacaa gattcaacaa ggatgctgat aaacagtaca ttcaaccaat
     6841 cgtatacaca aaagaaaaaa gtatcactta tgatgagacg acactaacag caattgagtt
     6901 aaagaatccc caggatgaac cgcatgaata taaagaggta cctattacag agtattctcc
     6961 taatcgtttt cggatgggct tgtatgaaga tgtactatct ttgattgatc tatacgatgc
     7021 agggcagtct gataccgcca attatatgac tgatctaaac gatgctcttc tagttattag
     7081 tggtgatatt gaagcagcag gactatccac agaggacgcc atcaagcaga aagaagcgaa
     7141 tatgcttttg cttgagtctg gaactgatgt gaacggtaat aaaacaagtg tgactgcagg
     7201 atatatttac aaacaatatg atgtgaacgg tgtagaagca tacaaataca gagtacgcaa
     7261 ggatattcac gaaatatcca tggttcctga tcttactgat gacaattttt ccggagtgca
     7321 atcgggagaa gcaatgaaat ataaattatt tggatttgaa caaatgacgg caacaaagca
     7381 aaggctattc aaaaaaggcc ttatgcggcg ttatcgtctt ttatttagcc taaaatcaag
     7441 tatttctgaa atggataact ccgatttgaa aggcttacgt gtaatattta cgcctaatct
     7501 acctaaagcc attctggaag agttgaaatc tttggttgat gctggagctg aactcagtca
     7561 agagacgatc ttaggactcg cttcttttgt tccagatgta caggcagagt tgaaacgagt
     7621 aaataaagaa acgcaaaagc agattggcat ttttgattca gatggtgaag aagtaattaa
     7681 caacaaaaaa gatgaaacag gggggtgatt aaatgaactc ccaagaatat tggatcaaac
     7741 gggaaaagga atggcaaaag caacaaatta aagatgataa aaagcgcatg gcagaaatta
     7801 aaagtcgcat gcaatacgca caagatgcga tacaaaaaga aatagacgcg cagtgggaca
     7861 gtttctccaa tggccagaaa atcactcgta gcgaagcgat gaagcgtgct agtgaaatgg
     7921 atgtcaaagc attcgctcgc aaagcaaaga agtatgtcaa agagaaagat ttttctccta
     7981 cagcaaacca agaattaaag ctatacaatc ttacgatgcg tgtaaataga ttagagctct
     8041 taaaagctaa tatcgggctt gaattgattt cactgtttaa tgaattggat aagtactttt
     8101 cgaatgaatt aacaaaagct ggtttagctg aattgaagag acaagccggt attttagaaa
     8161 tgactattgc ttcaagtgga tatgcaaagc tgatagaact agtaataaac agctcctttt
     8221 tgagtgatga cgtgtctttt agtgatcgct tatggatgta tcaatctgaa ttgaaatcag
     8281 aattagatag gttgttagtc agaagtataa cgatggggaa aaatcccaag caacttgcat
     8341 ctaaattggc agaatattta acagctgaag gacgagaaaa cactaagttc aacactcaac
     8401 gtttgatggt gactgaaacg actagagttc aggtagggat tcaagaacga agttatagag
     8461 atgcaggcat tacccagtac atctatatag cagaaccaac ggcgtgcaaa ctatgtatac
     8521 cgttaaataa tcaagttttt gatgttgccg atatgcagcc aggaagtaac gctcctaata
     8581 tgcatccatt ttgtcgatgc agtacagcac cttatataga acgaatatca agccgttaac
     8641 acaaattaac ggctttttat tgtgccttct tacagcttac aggcgttaaa gagaaagcta
     8701 ttttcggctg accggcgtaa ctggtcaaat ttatcgggta gcggcgtaac cgtggaggat
     8761 taatcatgaa aaaacgttta tttatgccaa tgaacttaca atttttttct gaaccaggag
     8821 atggtggatc tggtgatgag ggacaacaag gaaacctacc agctggctca caagagacac
     8881 cgcccgaagc aaaagaagaa aacaatactg gcaaaacatt ttctcgtgat gaagtagcga
     8941 aaatgatcgc tgctgagacg aataaagcaa aagcagcgtg ggaaaaagaa ctagcagcaa
     9001 aaaaagaaga agctaaaaag ctggcaaaaa tgaatgcgga agaaaaacta cagcatgagt
     9061 tggagcaaaa agaagctgaa atcgctgaat taaagcgtgg acaggcacta tctgaaatga
     9121 cgaaagaagc ttctaaaatg ctgacagatg caaatttacc acacgatgat gatttacttg
     9181 gtctgattgt ttctgatgat gcagatgcca caaaacaagc tgtagcagtc atcactaact
     9241 ttgcttcttt gattaagaga gaaaacgcaa gacaaacacc accaaatgaa ggtggacaat
     9301 ttacagcatc gaaaaatact aaagaaacag tggctaaact agctgctaaa aatcgaatta
     9361 tcaaatagga ggaaaactta atgaaaaaga aacaactttt accaatgaac ttgcaaatgt
     9421 ttgctcaaac atgggatcca gataatgtct tggtatatga aacgaaagag ggaaaaattc
     9481 ctgataaata taatacgctc attttgagtg aagttatgga aaattctaag atcatgcagt
     9541 tagcaaaata cgaagaaatg actgacaaag aaaagaaatt tgaatacttt gcagaaggac
     9601 caggcgcata ctgggtgggt gaaggtgaaa aaattaaaac gtctaaacct aaatggatgc
     9661 aagctacgat gactgcaaaa aaactcggtg tcattcttcc ggtttctcgt gaatatttaa
     9721 attataaatt atcagatttc tttgaggaga tgcagccaaa aattgctgaa gctttctata
     9781 aaaaatttga tgcagctgcc ttattaaata aagaaaaccc atttcctcag tcactagacg
     9841 gatcagttat tagtgcgggg aatgtggttg aaggcggatt gacttatgat aatatcctag
     9901 ccttagaaga caagttagca gaaaatgaat tcgaacctaa tgcgtttatt tcaaaccgaa
     9961 aaaatcgtac agaattacgt tctgcagctc aaacagtcgg gtcaaatgtt gagtttattt
    10021 atgatcgctc tactaataca attgacggat taccagtagt agaccttaag tctttagata
    10081 aaggaacact ttacgctgga gactttaatt acatgtttta tgggatccca tataatattt
    10141 catttaagat ttctgaagaa gcccaattgt ctactttaac taatgaagat ggaaccccag
    10201 ttaacttgtt tgagcaagaa ctgattgctt tgcgtgcaac aatggatgtt ggatttatga
    10261 ttgtaaaaga tgaagcattt gggaagattt ccccaaaagc gtaacgcctg ctaccggtat
    10321 tgtgccaaat caaaagacat ggaccggtaa agtaggcgat actaaaacat ttactatttc
    10381 agctgtgcct gcagatgcta gcgatgcagc tgctgttgtt gcagctacta cagcaacttc
    10441 aagtgatgga gctatcgcaa cagtgaccaa aaatgaaaat ggtggttttg atggaacgat
    10501 tgcagcagaa gggtcagcaa cattcacatt tacttctgga gaattcacta cttcaatcaa
    10561 tgtgacaggt caacctgcta gttaggaagt aaaaatatga tgattgcaga tgatattaaa
    10621 aaacttctta aaggaacaat agatgaaaag cttgaagtta ttgagcgaag aacgaatgag
    10681 cgtatgaaaa ccttgttaaa tacgcaagaa gttcctaaag aatttgaaac agttgtatat
    10741 gaagtatcgt tgaaaagatt caatagaatt ggtcaagaag gtatgcagtc atattctcaa
    10801 gaaggtttat ctatggcttt tcctgactcg gatttttcag agtatcaaaa tgagattgat
    10861 gaatttaagc gtaaagatca ggaagagttg tacaagccaa agcgagggag gtttaaattt
    10921 gtatgagatt tacagatgaa attatatttg ttaaacgttc atctgactct aaatatgatc
    10981 cagatctcgg tgagtgggtt gaaggcaaac cagaaagaac aagaacagag gcaaacgtga
    11041 cagatattgg cactgataga agtgtgacta tttttggtag tgtggaagaa ggggcgaagg
    11101 tcattaggac gcagcctctt ttttctatcc ctacatttga ctatatcgag attgaaggaa
    11161 agacttggca acaaaaaaca gctagaaatc cagcatatag aaatagttta attgtgcaag
    11221 aggtggttct tgatgaaggc acaacttgaa tataaaggaa tcgatcagct gatgcgacat
    11281 ctgaaaaaag cagcaacgct taatgacgtt caaaaagtcg tgaaaagtaa tactgctgaa
    11341 atgactgaac gaatgcaaaa aggtgcgcca gtggatacag gttacttacg aagatcaata
    11401 aacatgaatc ttttagaagc tggtttaact ggtattgtag gaccgacagc agattatgca
    11461 ccttatgtag aatatggcac tcgatttatg tcggcacagc cttatgttag accagcgttt
    11521 aattaccaaa aagtcaaatt tatggctgaa atgaaagcct tggtgaaatg atgattaaga
    11581 caagagatca atcaattttt gatgaacttt ttaaaatatc ccaaaacaaa cttggatata
    11641 aaacatacga ttacaaaact ttagaggatg ttggttatcc ttttgtggaa tttgagaaca
    11701 ctcagacaat ccatgaagta aataaaactg acattaaagg gtctgtgatt gtggttttat
    11761 ccgtttgggg attacagaag aaacgaaagc agttgtcaga tatggcatct gctcttttta
    11821 atgaagctag attgatagaa gccacagaag gctattattg ggctttaaat tatcaagcaa
    11881 gtggaattca agtgatggac gacacaacaa cgaatacacc gctaaaacgg gcggttgtca
    11941 cacttgaatt tagaattaga taggaggaag aacatggaag cattaaaagg tattgatgtc
    12001 attttgcttt atcgcttatt gaaaaaagaa actcaggaag ccgcttggaa aatggcattt
    12061 caaacagaac acgaaaatgg tttatcaaga gattcagact ctacagtgac aaaagatgga
    12121 aacgttcaaa gtttaagccc ggttgaatat gatttttcgg ctacttcaat agttgctaaa
    12181 ggagattctc atgtagatga aatgaaacaa gctttattaa atggggatat cattgaaatt
    12241 tgggaaatta ataaagcaga acagggaaca gatgataatg caaataagta caaagctact
    12301 tattaccaag catatgtgtc tgaatttact ccatcggctg ctgcagagga taacgttgaa
    12361 ttaagtttat catttgcagt aaatggtgtt ggtcaagatg gttatgcaac cttgacagaa
    12421 gatcaagccg atgttgttca atatgcattc aaagataccg tgaaagcaac ttcgacagga
    12481 gcataagagg gcttagatgc tctctttttt attttaggag gatgaaaaac attgaaatta
    12541 aaaattaaag gtaaagaata ttcgtttaaa tttggcacta aatttgtacg tgaattagat
    12601 aaagtgatgc ctttcgtcga tggaaatatg gaatttggaa tgggactctc cgcaaaagtc
    12661 ttaccagagc tacgttctta taacgttaac acgttatcac gagttctgga aatagcaaat
    12721 agaactgaag atgaatctat tacattagat gaattggatg attacatcga tgaagttaaa
    12781 gacattgaaa aattgtttga tgaagtccta aaagaattgg cggagtcgaa cgcgggaaag
    12841 ttagcggtcc gaaacctgaa tcagaaattg aaagaagcgg aaaaacaaca agtggaatag
    12901 attctgcact ggcatacgaa caaattctta tcaattcttt tcgatatttg ggaatgacca
    12961 atatctcaga tatcgaaaga atgacgttat atgaatacaa cattcgtatg actgcagccc
    13021 agttatcttg gcttgacaaa gaaaagttga ttcacgaatt agcgtgggca aatcagcaag
    13081 tccaagcgga gaaaaaagta ggcaaaaaga cagttcctgt atatcgatcc tttgaagaat
    13141 tcttcaatta tcaaaaaatc gaagattcaa tcatgggagt ttccgaactt tcaaaacaag
    13201 ataaaaaatt ccaaagctta ctaactaaag ctaactcttg aggaaaggag gagaatcatg
    13261 gaacaatttt ctgttgaagc cttattaaaa gccacagata gtggatttgt aaagactttt
    13321 aaagatgcac aagatgctgt taaaactttt gaaaagaatt caaatagtat gacaaccgct
    13381 gttggtaaag tgatgcaagg tactggtgcc gcaatgacaa agtatattac cactcctctt
    13441 ataggagtag gcgtagcagc tgctaaagtt ggtggcgact tcgaagcaca aatgagtcgt
    13501 gtaaaagcta tatcaggagc aaccggcgac acattcgaac agatgaaaca gcaagcgatt
    13561 gatctaggag caaaaactgc ttttagcgca aaagaatcag ctgctggaat ggaaaactta
    13621 gcttctgctg gatttagcgc acaagaaatc atgaaagcaa tgccgggtct tttagactta
    13681 gcagctgtat ctggagggga tgtggctcta gcttctgaaa atactgctac tgctttgaga
    13741 ggatttggtt tagaagcaag tgaagcagga catgtcgctg atgtatttgc tcgtgctgct
    13801 gcggatacca atgctgaagt tggagacatg ggagaagcat tgaagtatgt tgctcctgta
    13861 gctaattcaa tggggatttc tttggaagaa actgcagcag ctattggtat tatgagtgac
    13921 gcaggcatta agggttctca agcaggtaca acgttgcgag gagcattgtc taggttagca
    13981 aggccaacaa aggctatgca agatacaatg gataatttag gtgtttcgtt ttatgatgct
    14041 gacggtaaaa tgaaaccttt aaaaactcaa gtagaattac ttaaaaaagc ttttgaaggc
    14101 ctgacgcctg aacaacaaca aaatgcttta gtaacactat atgggcaaga atcattatca
    14161 gggatgatgg ctttgattga taaaggacct gattcattgg gcaaattaac aaaatctctg
    14221 aaagattctg atggtgcagc tgacgatatg gctcggacca tgcaagataa tatgaattct
    14281 tccatcgagc aaatgtttgg agcttttgag tcagcagcta ttgtaattca aaagattcta
    14341 gcaccatcca tcaaaaaagt agcagatgcc atatccggct tagtagaaaa atttgtaagt
    14401 gctccagaat caactcaaaa attagtagtg gccataggag caattgtcgc tgctatagga
    14461 ccgttaattt ttatgattgg ttcagtaatt atatggatca atagggtgaa agtagctttt
    14521 aaagctttaa gtgaaagttc aaaattgttt agtggattaa gtaaagcaat gggtcttctt
    14581 acaaatccgg tttttctggt tatagctgcg gtagcactac tcgttgtagg tttcatctat
    14641 ctttggaata cgagtgaaga ttttagaaac ttttggattg gcttatggga gggaatcaag
    14701 tctgctgtaa gctcggcagt agaatggatt cagaatgcat ggaaatctac aggagaatgg
    14761 tttaacaatt tatggaagtc cattaaagaa ggcgcagaca atgtttggac tacagttcaa
    14821 gaagcccctg ggaaagcggc agattggatt aaaaacaaat ggactggaac aaaagagttc
    14881 ttttcaaatt tatggtcaag tattgcaaac tctgcttcag agatttggaa tagtttaaaa
    14941 gaaggtgtca tatcagttat tgatgattta gtttcaagtg ctggtgaaaa atgggaaggg
    15001 tttaaaaata ctatatctac tgcatggaaa acaattacaa gtaaaatcaa atctggtttt
    15061 gattttatac taaaatatat tggtccattt gtaagtagct tttcagatgt gttctctaat
    15121 atagtgaaag caataacaag tatatttgct gaggttaaaa acataatagt aaatgcttgg
    15181 gaaatcatta agtctttaat agctgcgcca ctactgttta ttatagattt aattactggt
    15241 gacttcgaac aaatgaaaga ggatttagat ctaatctgga acacacttgt ccaatcagtg
    15301 gtaaatattt ggacatctgt aaaaaatata tttacggaat atatcggtgc aatagtaaat
    15361 agtgccgtta gtttatggac tggattcata caaagtattt ctaatatttg gaatgaagta
    15421 gtttatcaag cgactatgat t
//
LOCUS       DACCXM010000061        15085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCXM010000061 DACCXM010000000
VERSION     DACCXM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.063"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            61..2658
                     /gene="adhE"
                     /locus_tag="IVY61_001688"
                     /gene_synonym="adhC"
     CDS             61..2658
                     /gene="adhE"
                     /locus_tag="IVY61_001688"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP7970802.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2887..3993
                     /locus_tag="IVY61_001689"
     CDS             2887..3993
                     /locus_tag="IVY61_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7970803.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4010..5038
                     /locus_tag="IVY61_001690"
     CDS             4010..5038
                     /locus_tag="IVY61_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP7970804.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5044..5523
                     /locus_tag="IVY61_001691"
     CDS             5044..5523
                     /locus_tag="IVY61_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP7970805.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5539..6834
                     /locus_tag="IVY61_001692"
     CDS             5539..6834
                     /locus_tag="IVY61_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP7970806.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6845..7669
                     /locus_tag="IVY61_001693"
     CDS             6845..7669
                     /locus_tag="IVY61_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7970807.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7834..8733
                     /locus_tag="IVY61_001694"
     CDS             7834..8733
                     /locus_tag="IVY61_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7970808.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8859..9728)
                     /locus_tag="IVY61_001695"
     CDS             complement(8859..9728)
                     /locus_tag="IVY61_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7970809.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9881..11341
                     /gene="rhaB"
                     /locus_tag="IVY61_001696"
     CDS             9881..11341
                     /gene="rhaB"
                     /locus_tag="IVY61_001696"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP7970810.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11338..12594
                     /gene="rhaA"
                     /locus_tag="IVY61_001697"
     CDS             11338..12594
                     /gene="rhaA"
                     /locus_tag="IVY61_001697"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP7970811.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12608..13453
                     /gene="rhaD"
                     /locus_tag="IVY61_001698"
     CDS             12608..13453
                     /gene="rhaD"
                     /locus_tag="IVY61_001698"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP7970812.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13455..13766
                     /gene="rhaM"
                     /locus_tag="IVY61_001699"
     CDS             13455..13766
                     /gene="rhaM"
                     /locus_tag="IVY61_001699"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP7970813.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13848..14999
                     /gene="fucO"
                     /locus_tag="IVY61_001700"
     CDS             13848..14999
                     /gene="fucO"
                     /locus_tag="IVY61_001700"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP7970814.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
BASE COUNT         4839 a         2591 c         3419 g         4236 t
ORIGIN      
        1 attgtatgat atacatgtga attatttaac aaataaccat gacccattag gaggataatg
       61 atggctaaag taatagaaaa agaaaaaacg aagacaattg acgtacaagc aatgattgat
      121 gatttggcag aaaaagcaaa tgcagcattg aaagaaatgg aaagctttga tcaagaaaaa
      181 gtagaccata tcgtgcatga aatggcaatg gcagctttgg atcaacatat gccattagcg
      241 aagatggctg tagaggaaac aggccgcggt gtttacgaag ataaagcaat caaaaatatg
      301 tatgcgtccg aatatatttg gcataatatc aaacatgata aaacagttgg tgtgatcaat
      361 gaagatgtcc aaaaagggtt gattgaaatt gctgaaccag tcggtgtggt ttgcggagta
      421 actccaacaa cgaatccgac ttccacaacg attttcaaat caatgatcgc gttgaaaaca
      481 agaaatccaa tcgtttttgc tttccatcca agtgcccaaa aatcttctgc agaagcagca
      541 cgagtggtac gtgatgctgc tatcgctgcc ggagctcctg aaaattgtat ccaatggatc
      601 gaacatcctt cgatcgaagc gacttctatg ctaatgaacc atccaggtat cgcgatcgtc
      661 ttagcgacag gtggagctgg aatggtcaag tctgcttact ctacaggaaa accagcttta
      721 ggtgtaggtc caggtaacgt accagcttat attgaaaaaa cagcaaaaat taaacgtgca
      781 gtgaacgatt tgatcgtttc gaaaacattt gataatggaa tgatttgtgc ttctgaacaa
      841 gctgtgatcg tggataaaga aatctatgca gcagtaaaag cagaattcca agcacaccaa
      901 gtttatatcg taaaaccaga tgaattgcaa aaattagaag atgcagtgat gaacgaagga
      961 aaatatgcgg tcaatccatc aatcgttgga cattctgcga tggaaatcgc aaaattggca
     1021 ggaatcagtg taccaaaagg aacgaaaatg ctgattgctg aattggaagg cgtaggccca
     1081 gattatccgc tatcaagaga aaaactttct ccagttcttg ccatgatcaa agctaacaac
     1141 acggaccatg cgtttgattt gtgtgaaggt atgttgaatc ttggaggatt aggacataca
     1201 gcagttatcc attcagaaaa cgaagagttg catgtcaaat tcggtcttcg aatgaaagct
     1261 tgtcgtattt tagtgaatac accatctgca gaaggcggca tcggtgatat ctataacgaa
     1321 atgattccgt cattgacttt gggatgcggt tcttatggta agaactctgt ttctcgtaac
     1381 gtctctgctg taaacttaat caatgtcaaa actgtagcga aacggagaaa taacatgcaa
     1441 tggttcaaat taccgccaaa aatcttcttt gaaaagaact ctctattgta cttagagaaa
     1501 atggaaaacg ttgaacgtgt catgatcgtt tgtgaccctg gtatggtcca attcggttac
     1561 tgcgatacag ttcgtgaagt attggcacga cgtaaaaatg atgtgaagat cgaagtcttc
     1621 tcagatgttg aaccgaaccc atcgacaaat accgtatacg caggaacgaa actgatggca
     1681 gacttcaagc cagatacagt aatcgctctt ggaggcggtt ctgcaatgga cgcagctaag
     1741 ggaatgtgga tgttctatga acacccagac acatctttct ttggtgcaaa acaaaaattc
     1801 ttggatatcc gtaaacggac atataagatc gacaaaccag aaaaaacaca attcgtatgt
     1861 atcccgacaa catcagggac tggttctgaa gtaacgccat ttgcggtcat cactgacagt
     1921 gaaacacatg tgaaatatcc gttagcagat tatgcattga cgccagatgt cgcaatcgtc
     1981 gatcctcaat tcgtcatgtc agttccagca tctgtcacag cagataccgg gatggatgtg
     2041 ttaacacatg caatcgaatc ctatgtttca gtcatggctt cagattatac acgtggattg
     2101 agcctacaag caatcaagtt agtctttgac tatttagaaa aatcagtaaa aacacctgat
     2161 atggaatcac gtgaaaaaat gcacaacgct tctacaatgg ctggtatggc gtttgctaac
     2221 gcattcctag gtatttgtca ctcagtcgca cataaaatcg gcggggaata cgggatccct
     2281 catggacgga caaatgcgat tcttttaccg catattatcc gctataatgc aaaagatcca
     2341 caaaaacatg cgatgttccc taaatatgac tatttccgtg cagatacaga ttacgcagat
     2401 atcgcaaaat tcttaggtct gaaaggcgaa acaacagagg aattagtcga agctttagca
     2461 acggctgttt atgaattagg aaaatctgtc ggcatcaaca tgagtctaaa agctcaagga
     2521 gtcacacaag aaacactaga tacaaccgta gaccgtatgg ctgaattagc ttacgaagac
     2581 caatgtacga cagctaatcc gaaagaacca ttgatctctg aattgaaaca gatcatcatt
     2641 gatgcttaca acggataagc aaaagaaagc gtagaaaaaa gataagaaaa tagatgaaaa
     2701 agaacttgga aaacgttgtt tctccaagtt ctttttttaa tttccaagta actgaaagtt
     2761 acttttgtta tttaacattt aggtcaattt tagacagttt ttgttcaaaa agcgatattt
     2821 atagtggacg gtattactgc tatattaagg atataaaatg aaagcgttta aaaaggtggc
     2881 gtaaagatga ttattaaaga agagattttt gtacctaaag aattagtgaa agaaaaaatt
     2941 gacctgctcg ttgaaaacct gacaacgatc aaagacgata atggtgaatt tttgttggac
     3001 tttgatggac tgaaagtaga tgacaaaagc tggacagtgt ggaattggcc acaaggtgtc
     3061 ggtctatatg gtatttataa aaattaccag atgacaaaaa atcctcgcgc ttatcaagtg
     3121 gtcaatgaat ggtttgagga tcgtatggaa gaaggcgctc cgcccaaaaa cgtcaataca
     3181 atggcaccgc tattgactat ggcttacttg tatgaagaca cgaaagattc acgctatttg
     3241 ccttatttag aacaatgggc agaatgggtc atgaatgaca tgcctcggac aaatgaagac
     3301 gggttacagc atgccacata tggaccggaa aataagaatc agttgtggga cgatacattg
     3361 atgatgacag tgcttccttt ggcaaaaatc gggaaactgt tgaatcgccc agaatattta
     3421 gaagaagctc gttaccagtt tatgatccat atcaaatact tgatggataa acgaacagga
     3481 ttgtggtatc acggctggac gtttgaagga aatcataact atgccgaagc attttgggct
     3541 cgtggaaact gctggttgac gattgctatt ccagagatca ttgaaatctt agaattatcg
     3601 aaagaagatg cattgcgcaa attattgatc gaaacacttg aagcacaagt gcgtgcatta
     3661 aaaacttatc agtctgaaag cggtttatgg catacgttgt tggatgatcc ttcttcttac
     3721 gtagaatctt ctgcaactgc aggatttgct tacggtattt tgaaagcggt ccataaacgt
     3781 tatttaccac aagaatataa agaagtcgct tatcgtgcta tccaaggatt gcttgaacaa
     3841 atcgatgaaa aaggagaagt tcaaaacgtt tcaatcggga caggaatggg ggacagttta
     3901 gatttttatc ggaatatcgg aatcacagca atgccttatg gacagtcgtt aacggtgctt
     3961 tgtttgacag aattgctcgt ctcttattgt taaaagaaag gaaacccata tgaaaaaaac
     4021 atcactgctg tttgcagcaa gttcgcttag cctaggtctg ctaggtctaa gcggctgcgg
     4081 aaataattct agtgtggcaa ctgtcgcaga tgcagatgta aatgagatag ttaccttgcg
     4141 ctttgcttat gcaagcaaca gccagccagt aattgatgca atgaatgaat ttgggcgttt
     4201 agtaaaagaa aaaacagatg gttccgtaca gatccagtat ttcccagatg gacaactggg
     4261 tggagaacgt gaactgatcg aattgacaca gtcaggagca gtagatttca caaaagtaag
     4321 tgcctcagcg ttggaaagtt tctctaaaga ttattcgatt ttttcagttc cttatctatt
     4381 caatgatgaa gaacatttct ttaaggtgat ggataatgaa gaaataatgg agccaatcta
     4441 ccaatcgacc aaaaatttag gatttaccgg attgacctat tatgattctg gtcagcgcag
     4501 cttttatatg gtagacggtc cgatccatac gccggaagat ttgaagggaa agaagatccg
     4561 tgtgatgcaa agcgaaaccg ctatcaaaat ggtagagctt ttaggcggtt cgcccgtacc
     4621 tatgggaagt gatgaagtgt acacgtctct tcaatccaat ttgatcaacg gatcagaaaa
     4681 caatgaattt gtcttgtata cagctggaca tggtggtgtg gctaaatact attcctatga
     4741 tgagcataca cgtgtgccag atatcatcat catgaatgat gccatcaaag aacagctaac
     4801 tgatgagcag gaaaaagcca ttgaagaagc agcgaaagaa tcaacggttt ttgaaattga
     4861 agcttttgca aaagcaattg aagaagaaaa agctttagca caagaagaac atggagtcca
     4921 attcaatgat gtagataata caccgtttcg agaggcagtt gccccacttc atgatatatt
     4981 caaaaacgat cctacctaca aagaccttta tcaaaagatc caagagcaag gagcgtagct
     5041 gtgatgaaaa tgattttaaa caaggtatta gagatcctgg gagctgtttt gcttgttggg
     5101 atggtcgctg ttgttttatg gcaagtattt tccagaacgg ttttaggaaa tccaaatacg
     5161 gtcacggaag agcttgttcg attcggtctt gtctggtttg ccatgttgtc ttccgcgtat
     5221 gttgtgggac aaaaagcaca tttagccgta acggttttaa gtgaaaaatt aactggcaaa
     5281 aaaagtaaaa ttttagaagt tgttgttcaa agtttatttc tggtatttgc tgcgatgatc
     5341 atggtgtatg gcggatggaa tgctgtcaca ttgacaatgg ggcagatctc gccttcttta
     5401 agtattccta tgggatatgt ttatctgtca gtacctgtat cgggggtact catcattatc
     5461 tatagcctga tcaatctaat cgatacatta agcaacaagc cagcaattca agaaaaaata
     5521 taaaaatgga ggaataaaat ggcaatagaa gccggattag tgttattttt tgtatttgtt
     5581 gtgttgcttg tattagggat gccgattgct ataagtattg cattctcatc gatgatgact
     5641 ttattgttag ttataccgtt tgatgtttct gcttttagtt cagctcaaaa aatggtggga
     5701 agtatcaata gtttttcgct gatcgctatt ccgtttttcg ttctctcggg cattatcatg
     5761 aataatggag ggatagctaa gaaactagtg gattttgcga tgttgttcgt cgggcgcgtg
     5821 ccgggggctt tggctcatac gaatgtgtta gggaatgctt tatttggttc catctctagc
     5881 tcggccattg ccgcttctac cgcgattggc ggagtgttga tccctcagca agtggaagaa
     5941 ggatatgacc ggaagtttgc gacagcggtc aatatcgcgt cagctccgac aggtatggtc
     6001 attcctccaa gtactgcttt tatcatgtat tcacttgttg ctggtggtgc ttcgatctct
     6061 gctttgttta tgggaggtta tctcgtaggc gctttgtggg cgctaggaat catgctagta
     6121 gcattcatcg ttgctaaaaa gaataaatac cctgttgtcg aaaaaggaca gatggctcat
     6181 gctggaaaaa taataaaaga ggcgattcct agccttctat tgattgtcat cattatcggt
     6241 ggtatcttga cggggatatt tacagcagta gaagcttcag cgatagctgt catttactcc
     6301 ctgttgattg ctatgtttta ctataaaacg gtaaaactaa aagatatacc taatatgttg
     6361 tttcaatcag tagcaatgtc agggacgatc atgttcttat tggctacttc ttccatgatg
     6421 tctttcgcca tggcgtttac cggtattcct caggcaatta gttcgttgat tatgggcgta
     6481 acggataata aatttattat tctgttatta gtcaatcttg tgttattact ggtgggtatg
     6541 tttatggatg tggcgcctgc tatcttgatt tttacaccaa tatttttacc aatcgtcaca
     6601 agtgtagggg ttgatcctgt acattacggg ttgttttcaa tcatgaatct gtgtgtaggc
     6661 tccatcacgc cgccagtagg tacaggacta tatgtcggtg caagtgtagg aggcgtaaaa
     6721 gcagaggcaa tgctgaaacc gcttcttcct ttttatgggg ttatcttagt cgtcttgttt
     6781 ttgattactt acttcccgca aatcgtcatg tggctaccca atatgctgag ttaggagaat
     6841 agttatgttg aacattacaa ttagaggaca tgatctttcc gaagtccaaa cgatagaaga
     6901 tcttgcagaa aaaacaaaag aacaagggat tcatacactg cagctcgcct taggtctttc
     6961 gtttcctgaa ataccttctg gcgcaaatga gatcaattct gggatgggaa attatgtcaa
     7021 acgagtttta gaaaagcaag aagtgaccgt aggaatttta agttgctata tcaatatgat
     7081 tcatccagat ttaacgatac gacaagagtt gctgacaaag ttcgagcagt atcttcgcta
     7141 tgcttcttcc tttggtgcgt cgatggttgc ttcggaaact ggatgtgttt tgccggaaat
     7201 ccaatatacg gaagaaaatt tcacagatga agcatttgct gaagctgtgt ccgttatccg
     7261 tcgcttggta aaagctggag aaaaatacca aatgatggtg gggatagaac caggtttgaa
     7321 tcatccggtg tattctttag caagggtaga gcagctgatc caagcagtag attcggacta
     7381 tctaggaatc attttagatc cgaccaattt gatcacaagt agtgattatc aagaacaagt
     7441 ccaattggta gaagaagctt ttgaacgttt tggtgaaaag atatgtgcgg ttcatttgaa
     7501 agattttcgt gtcgaacagg aaaaaatcgt gccggtaaat ttgggagacg gtgtgatcga
     7561 gtacacaaaa atcaaagaaa tcatcatgaa gaatcgtccg tacctgtatg tggttcttga
     7621 agaaacaaaa gatgatggga ttcgttatgg aagaagtctt cttgaataat gtaaagaaga
     7681 cgttggttaa ctaatattta gaatggaggg ggcggggcta gaagatgcca cgtctcttct
     7741 tttttataag agtatataaa agcagctctt tttgattaca aaaaacgtgg tataataaaa
     7801 ttgtgcataa gtgaacaaaa ggaggtgcgg cacatgctcc aaaagaaaat cagcggtgaa
     7861 caagtcaata cgatggtttc tgatcagtat gaaatctatc atgtcaaaga tgtcgtcagc
     7921 caaaataaaa cgatctatca tcatcatgat ttctatgaga tccatgctac attaaaggga
     7981 gaagcgactt tttatttgaa cggacgacaa tttacgatca aagcaggaag tgtgctgctc
     8041 atccattcca atgatttgca tcgtatcgtc cgccaatctt cagatgtatt cgagcgtgtc
     8101 tatatctttg tcaccccttc ttttctagaa agtcgttcca cgccatggtc taatctatcg
     8161 gcttgttttc aaccaatggg agagaagagg agtcgtgtat tgcaaataga acaggaagta
     8221 ttgaagcaaa aattatcatt tatcgaccat cctcctgata aaaaaattta cggtgaagac
     8281 ataaaatatg aacaagctct ggtagaatac ttgatttttc tcaatcaagt tgtacaaaaa
     8341 gaagagagtg cgcaagaaca gaatgctgta gttcctaatg agcgtgtgga acaaatgtta
     8401 caatatatct caaaaaattt atctcatccg ctttctttag aggaaatgga aaagcagttt
     8461 tttgtcagca aataccatat cacacgagaa tttaaaaagt acactggatt tacgtttcat
     8521 cagtatgtaa tgaagaaaaa gctgatctat gccaagcact tgttgcgaga ataccgcagc
     8581 tctagtgctg tatatagtaa aagtggtttt tcttcttatc cgcattttct gaaagcattc
     8641 aagagagagt tcggcataac gcctaaagaa tttttgaaac gtgatacaca gaaacagttg
     8701 gttcattatg aacattttga agatgaacaa tgagaatgat cactaaaaaa taaccgcagt
     8761 agctgctgtt gtttacatag aagcagaaac tgcggttatt ttatttttat ctatatattt
     8821 caaaatattt tgaaaaaatg acgtacatat tatatgaatc agctgaattt acgaaagtct
     8881 ctcggcgata gtcctgtttt tgctttgaac aggcggctaa agtaagcaaa attctcaatc
     8941 cctatttctt cggaaatcat tcgaaccgta aattctgtat accgaagaag gtctttagcg
     9001 taagaaagtt ttttgctgat caaataatcg attggtggag aaccgataaa tttggaaaaa
     9061 tctttgctca gctgatattt attcaataag aattgttgtt ctaattgatc aagtgtaatc
     9121 gtttctttga aatgttcgtc taagtattgc ttcagtttgt aaacgtaggc gggaatatcc
     9181 tcttcttcga aatctagctg atacttttgc aaaatcagtt cattcagcaa ctcgtggata
     9241 gctacagagg actggaaatc ggtacgggca ttcgtgcctt gttgcatgtg gatcaattct
     9301 gtgatcaaat ggtggatctt gttttctgat ggatctcctg ttgaatggaa ggtgttatgg
     9361 ccgtcttttg tgaactcctg ataaaatgat tggacgttct gaccattcaa atggatccaa
     9421 tccatttccc atggttcttc gctgatcgtt tgataacgct gatattcttg gcagtcaata
     9481 aaaaataagt ctccagcttt tagctgttgc tgattatcat tatatgtaag cgaacctttg
     9541 ccagacaaag taaactttat caagtaagaa ggtaaatgtg ctcgttcagt atagtaaggg
     9601 attggtgctt tgaagtaacc gatttcttgg atataaaaaa ataaattcct gcttctttgt
     9661 gtaggcgtca agatcactct ttttgagtct tccgaccatg agtggttggc tttttcccag
     9721 taatccatgt aaacactccc aacttgtcaa gataatgcaa ttatatacca aaatagtgtg
     9781 aagcaaaata taagattctg tattatgata gcattgtcaa taaatgataa cgctttatta
     9841 ataaagtgct aatcaacaat aaagtaaaag aggtggagaa atgaatccaa catttgttgc
     9901 catcgatatc ggtgcatcaa gcggacgact aatgaaaagt acgagggatg caaaaggttc
     9961 tttgatcttg gaagaagtac accggttcaa aaacggcttt cggaacaaag acggtcacga
    10021 tcgctggaag atcgattatc ttatcaaaga gattttgatt ggatttgaaa aattaaagca
    10081 gacagggatt gatcgccttt ttgcggggat cgatacatgg gcagtcgatt actgtctgat
    10141 tgatgaacaa ggggatcgga taaatgatcc gattgcttat cgggacaagc gtaccaatgg
    10201 agcagtagaa cagttttctg aaatctattc tctagataag ttgtatgagc agacagggat
    10261 tcagatccag cctttcaata ccttgttcca attgtttgct gaagaaaaag aaaatttggc
    10321 aaaagcacat aaattgttac tgatacctga ttatttaggg tatgtcttta ctggtaacat
    10381 ggtgacggag aaaactaatg catctactat gcagctgctg aatgcaggga caaaagaatg
    10441 ggaaatagaa cttcttgaaa aaatcggtgt ctcgaaggaa ttatttccac cattgacaga
    10501 gccgggaacg atactaggag atctgcaaaa agaacaattt tcaaaatatg atttgccaga
    10561 tgtgaccttt atcacagtag caagtcatga tacggcgtca gctgtattag gaacgccagg
    10621 tcaaggagaa cgttggggtt atatcagcag tgggacatgg tcgcttttag ggattgaaac
    10681 aaagatcgcc aatgtttctc gcaatgcttt ttctgaaaat tatacgaatg aatggggtgc
    10741 ccataacacg gtgcgttttt tgaaaaacat catggggatg tggttgatcc aggaagttgc
    10801 ccgaatgcaa gagtacgagt acagttatgc ggaattggca gagcttgctg caaaagaaaa
    10861 accgctgcag caagtgatgg aaatcaatga ccctcgattt ttgaatcccc caaatatgat
    10921 agaagagatt cagcaatact gttttgaaac agagcagcca gtccctgtct catcgggagc
    10981 tatcgctcgt tgtgtgtatg ataatcttgc tctttgttac gcacaagagc tgatcaaagt
    11041 agaacactta actggaacga aattggaaaa gctgcacatc gtagggggcg gatcgaataa
    11101 tcgtctactg aatcaattga cagcagatta tgccggaatc attgtagaag caggacctag
    11161 tgaagccaca gcaatcggga atgttgccat gcaaatgctc agtacaggtc agttttcgaa
    11221 tatagaacaa gcaagaaatg taatcaaaca gtcgtttcca tgtgaaactt tttacccaga
    11281 tgaaaataag caaaacacga tggaatggta cgtgaataaa atcaaaggag taatcgtatg
    11341 aacacgattg aaagaaatta cgaacaagca aaagaaaaat atgcaactat cggtgtagat
    11401 acagatgctg tactagaaaa gatacaggac atcaaaattt ctatgcattg ctggcaagga
    11461 gatgatgtca aaggtttctt gacaccggat ggcgaattga ccggtggaat catggcgaca
    11521 gggaattttc caggagccgc acgaacacct gaagagctta gacaagatct ggagaaggct
    11581 tattcgttga taccgggcaa acacaaattg aaccttcatg ctatctattt ggatacagaa
    11641 gaatctgtcg atctaaatga aattgagccg aaacattttg aaaaatgggt agagtgggca
    11701 aaaaaagaag aaatcggtct tgacttcaat ccgactttct tttcgcatcc aatgatgaaa
    11761 gacggcttta cattggctca tccagataaa gaagttcgtg aatactggat cgaacacggg
    11821 aaaagaagcc gcaaaatcgc ggagtacttt gggaaagaaa ctgggcaaac aagtatcaat
    11881 aacttttggg tacctgatgg gatgaaagat aatccgattg atcgttttac cccacgggaa
    11941 cgtttaatgg aatcactcga tgaaattttt acagaagagt tagatgaacg gtatgtacaa
    12001 gatgcagtag aaagcaaact atttggttta ggcgctgaag cttatacagt cggttcccat
    12061 gaattttata tgggttacgg gttgactcgt gacaaacttg tttgtctaga tgctggacat
    12121 tttcatccaa cagaagtgat ttccaataaa ttatcttcat tagctttatt cagcaaagga
    12181 atcatgttgc atgtttcccg tccagtgcgt tgggattcag accatgtggt tttgatggat
    12241 gatgaattgc aggaaatcgc taaagaatta gtccgtaacg aattgttgga aaaaacaaat
    12301 atcggtctgg atttctttga tgcaacgatc aaccgtatcg ctgcttgggt catcggtacg
    12361 agaaatacac aaaaagcact gctgaaagct atgctggaac cagttgagcg attgaaagaa
    12421 atggagctgg cttttgactt tacttcaaga atggcttata cggaagaatt gaaagatttc
    12481 ccgtatgccg atgtatggaa ttatttctgt tacaaaaatc aagttccagt tggtttggat
    12541 tggctggaag aagtccagga atatcaaaaa gacgtgttgg aattaagaaa ataggagtgt
    12601 ggctatattg aaaaaaatag atattttaca agcgccttat gtgaaagaaa tgatcgaaac
    12661 gacgtcgaat ctttatcgct tgggatggga tgaaagaaat ggcgggaata tttcttattt
    12721 actaaaagaa gaagagatca caccgttctt ggatcctgaa aaagtgatcc gccgcatctc
    12781 gatgattttt gatgccagcc gtttagcagg atgctatttt attgtaactg gttcaggaag
    12841 atattttaaa aatgtagcga gcgaacctgc cgagaatctt ggaatcgttc gagttgctga
    12901 agatggcaaa acactggaac ttttgtgggg gctggaaaac gaatgccttc ctacaagcga
    12961 gttgccaagt catttcatga gtcatatcgc ccgtttagaa gtagacccag ataatcggat
    13021 cgttatgcat tgccatgcta gtcatctatt agcgatgagt tttacgcatg aattagacga
    13081 acgttccttt agtcgtacgc tatggcaaat gtgtacggaa tgcttagttg tttttccaga
    13141 aggaatttcc atcattccgt ggatggttcc gggaacaaat gagattgggg aagcaacagc
    13201 tgaaaaaatg aaagaaactc gattggtttt atggccacaa catggaatct acgggtcagg
    13261 aaaagacatg gatgaagtct ttggtttgat cgagacagca gaaaaggcag cagaagtcta
    13321 tacgtacgtc aaagcgcaag gaccaatctt gcaaacaatc acggacgaaa acctgtggca
    13381 tttagcagat gcgtttggtg tcactccaaa agcaggttat ttggaggaag ttcatacgaa
    13441 agcaggtgtc tgacatggtg aaaaaagctt tttgcatgaa agtttaccca gaaaaacaag
    13501 aagaatacaa aaaaagacat gatgaacttt ggccagaaat gcgtacagca cttcgtgaac
    13561 acggggtcat cgactattcg atctatcttg atccgagtac aagtttgtta tttggttact
    13621 tagtgatcga agatgaaaag ttatgggaaa aaatggcaaa tacatctatc aatcaaaaat
    13681 ggtggaagta catggaagat atcatggaga caaatccaga tgcctctcca gtcagttggg
    13741 aattgatgcc agtctttgaa ctgtagcaac aagcaactgg gcaaccggtt gcttggggtt
    13801 ctttcgtttt tattgtgaaa aataatacaa attaggagga atagaaaatg agtaatcgaa
    13861 tgattttaaa tgaaacatcg tattttggaa aaggagcgat tgagtcgatc gtccctgaat
    13921 ttcagcagcg aggattcggc aaagcagctg tcattactga caaaggattg atcgagcacg
    13981 gtatagcaac aaaagtaaca gaacttctag ataaagcagc aatcgcttat gctttatacg
    14041 acgggatcgt acctaatcca acgatccaaa atgtaaaaga tggagtggct tttgttaaag
    14101 aggcagaggc agactgtttg atcgcaatcg gtggaggttc gccaatcgat acggcaaaag
    14161 ccattgggat tattttgacg aatccagagt tttcagacgt cattagttta gaaggtgttg
    14221 ctgatacgaa aaatccatgt ttacctatat tagctatacc aacgacttct ggaacagcag
    14281 cagaagtaac gatcaactac gtcatcacag atgaagtaaa tcaccgaaaa tttgtttgtg
    14341 ttgatcctca tgatatccca atcgtcgcgt tcatcgacag cgatatgatg atgggaatgc
    14401 cgaaaaaatt ggctgcatca acaggtatgg atgctatgac gcatgctatt gaagggttta
    14461 tcacaaaagg cgcttgggaa atgactgata tgctgcattt aaaagcaatc gaaatcatcg
    14521 gccattcttt ggaagcttct gtagatggcg atcaaaatgg gcgagaaaaa atggcattag
    14581 gacagtatat tgcgggaatg ggcttctcaa atgtcggttt aggactggta cacgggatgg
    14641 ctcacccgtt aagtgcatgg tacaacattc ctcatggcgt agcgtgtgca gcacttttgc
    14701 caactgtcat gaaatataac aaagaatata ctggagaaaa gtaccgtgag atcgctcttg
    14761 ttctagggat taaaggagca gctgaaatgt ctttagaaga tgtaagagaa gcggcttgtg
    14821 gtgagatcga tcggttgagc aaagcggttg gaatcccaga aaccatttct gaattaggtg
    14881 taaaagaagc agatattcca gcaattgcag aagatgcttt gcgagatgta tgcacaccag
    14941 gaaatccaag agaaacgaca gtagaagaaa tcattgcttt atatcaaagt ttgatgtaaa
    15001 aacgaagcgg atcttcaaca ttaaattgcc tgtaaagaga aatggtagtt tatatctgga
    15061 tatctctttt aaattgatgg tattt
//
LOCUS       DACCXM010000062        14890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCXM010000062 DACCXM010000000
VERSION     DACCXM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.064"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(60..1613)
                     /locus_tag="IVY61_001701"
     CDS             complement(60..1613)
                     /locus_tag="IVY61_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7970815.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            1761..3050
                     /locus_tag="IVY61_001702"
     CDS             1761..3050
                     /locus_tag="IVY61_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7970816.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(3176..4255)
                     /locus_tag="IVY61_001703"
     CDS             complement(3176..4255)
                     /locus_tag="IVY61_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7970817.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(4364..4804)
                     /locus_tag="IVY61_001704"
     CDS             complement(4364..4804)
                     /locus_tag="IVY61_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7970818.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(4861..5271)
                     /locus_tag="IVY61_001705"
     CDS             complement(4861..5271)
                     /locus_tag="IVY61_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7970819.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5452..5886)
                     /locus_tag="IVY61_001706"
     CDS             complement(5452..5886)
                     /locus_tag="IVY61_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7970820.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(5986..6834)
                     /locus_tag="IVY61_001707"
     CDS             complement(5986..6834)
                     /locus_tag="IVY61_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7970821.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            7223..8989
                     /locus_tag="IVY61_001708"
     CDS             7223..8989
                     /locus_tag="IVY61_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP7970822.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            9091..10350
                     /locus_tag="IVY61_001709"
     CDS             9091..10350
                     /locus_tag="IVY61_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7970823.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            10497..11798
                     /locus_tag="IVY61_001710"
     CDS             10497..11798
                     /locus_tag="IVY61_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7970824.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            11801..12652
                     /locus_tag="IVY61_001711"
     CDS             11801..12652
                     /locus_tag="IVY61_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7970825.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            12665..13480
                     /locus_tag="IVY61_001712"
     CDS             12665..13480
                     /locus_tag="IVY61_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP7970826.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            13631..14797
                     /locus_tag="IVY61_001713"
     CDS             13631..14797
                     /locus_tag="IVY61_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7970827.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         5072 a         2640 c         2741 g         4437 t
ORIGIN      
        1 cttgatacat aaatccggag gttgttaata agcattcgga tttatgtatc ttatttggtc
       61 taggaagtaa cttgattttc ttttttcgat tgatccatga gtgcttcttc ttttaattca
      121 aagatatatt ctgagatcct cataatcact tttttccagt cttctggtgt gatgattcct
      181 tttaaaatat agattttttt gatatgagaa ggcaattgtt tcaaagggca gtcgctgata
      241 acgatgttgc agttctccat aaaatcattt tgtattttga tcgattcatt tgaaaaaatt
      301 tgttctatct taacgatcag atctttactg atataaaaat cacgcgtata gttcacacca
      361 atcacaattc gcggctgttt tttgtagcga tcatacaaat gtactaggtc ttcaaccatc
      421 cattgcacat tttccttttc taaaataact ggtaacaagt gttgattttg ttctctgaaa
      481 tccatataaa attgttcaat ggcggtttgg acttctttca acacgctatg ttcttgataa
      541 agcatgaaag taggaagggg cgtatttggt tgattgaagt taagaaatcc attataaagt
      601 ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt tttcaggtat aaacaaatca
      661 aatacagtac aaaaggactc caccatttct cttgaataaa gaacatgagg aacgtttgct
      721 ttttttaaaa gtttgtattg ctccaagctt gtttcttccg attcgatttt cgtgatagaa
      781 agtcgagcta aaatattcac taagatgcgt tgttcgcgac aaattggcag attaggtcgc
      841 aaaatatcta cagagggatc aataaaaaag tcaatatatt catactctgt gaattctttt
      901 tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac atatttttaa tagaaaatat
      961 aatttatcta tcataccgct atttagtcgg tcaagtactt cttttttaaa aaaagtattc
     1021 atcataaaag ccactacttg cggatcttca tcataaaatt ctgtagcagt acttgaaaag
     1081 atgttccaat aaatatctaa tgctgcatat tgaatttgta cctcttttcc agaaaaagac
     1141 tttctgccat tattagaaaa ggagatagac aaaccgtaca atgctaaaaa tgcattaatc
     1201 tttttcattt tcgtatacaa ataagaggca ctatagtttg tttgctgact taaactgata
     1261 atcgaatttg tctgacagct gaacaaagaa gtcaagatct gataactgac tgattcgttg
     1321 cagtatcttc caaatagacg ataatagcag taagccggat caattccttc aaaattcaac
     1381 tttaaaaaag gtttttgctt atcaatatgc agttctgtta gattgagacg ctcaaagtct
     1441 tgttctaatt ctttgatgta tagtaaaaaa tttgattcag acatatggaa atgatctgct
     1501 aaatattgtg tagtaataaa gaaacgatgt tcagatagga aaagaaaaat atctaatttt
     1561 gtattgcttt ttttcttcaa gagaaacgtt ttaatcgctt cttgagtttg catgaaagag
     1621 gctccttttt acttaaattg ttaaaacttg aaaatattac tgtttttttc ataaatttag
     1681 acaaaaaaac agtaaacata taggtagaat ctctactatc ttatttcttg catacaaaaa
     1741 aatcaaggag tgagagagtt atgagatatt caaccatctt aatatcaaca tttgcagcct
     1801 taagcttatc gcttactata aatcagacag tccacacaga ggagatagaa acagccaaat
     1861 ggacagaaac caagtgggcc aacgtcaatc gaattacctt tcaggatgaa ttttcatcct
     1921 tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc gaccatcaaa gcaaaaattg
     1981 actattcagg agacttagca cctatactac aatcagctgt tacatttgag aatgttgatc
     2041 ccgctttgtc gattggctat gacgagaaca ctttgactat ccaacacaag caagcgatat
     2101 ttaccttcac cgttacatta aatcaggact tgttaaaacc agcgcttttt actgtcaagg
     2161 tatcagatgg actaccgaac gatcatcatc atttaacgcc gtatagtcag cggcagacaa
     2221 ttgagcaaga ttcagctatc gactcaggag gagatcttgt tgaagaacca acagataaac
     2281 cagaaaatga aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac
     2341 cggaaataga acctggagaa gaacccgata cagaaactca accagagcca gacaatgaat
     2401 caaaacctga aataacccct ggagaaaaac cagatgtaga cccagaagaa aaaccggatg
     2461 tgactccaga accagatacg gattctggaa accaaacagt accggaaaca aatccagaca
     2521 cagataatga aactgaaaat ccagaaaaac cagaggtagc cccagaagaa aaaccagatg
     2581 taactccaga accagatacg gattctggaa accaaacagt accggaaaca aatccagaca
     2641 cagataatga aactgaaaat ccagaaaaac tagaggtaga tccagaagaa aaaccagatg
     2701 tgactccaga accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa
     2761 aaacaattca agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa
     2821 taaatgaaga gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa
     2881 ccttaaaaag tagcaaggat acaacctttg ctagtcctga aacgaaaaat aaacagctac
     2941 caaaatccgg agagtcacaa aacaaagtta tactgtggag cggaatcatt ctattaagta
     3001 tagcaacaat gctttctgca aaaagattta aacaaaacag atctctctaa catttgaata
     3061 atgtctgtga acgcattttt caaataaaaa gaaatatcca gaaaacgacc ccaaaatacc
     3121 agattgaaat ctgatatttg gggtcgttca tactttatct ttcttcatat ttattttaag
     3181 ggatatctgt ttcttttgga agatggttcg ggatcttttt cactttcaat acatctaaaa
     3241 agaaggtaaa gatcggaatg ccgacaatca agccccaaac gccaaagaac cgttcgctaa
     3301 caagtaagac cacaaatgta taaaaaatcg gcagttctgt tttgctggac atcaacttcg
     3361 gattcagcac ataagactca attagatgga caatcaaaat cgtaatcagg atataaacaa
     3421 catctcggat gcctccttgg gaataagcta tgaaagatag aggaatacaa gaaatgataa
     3481 cacctgctac tgggatcaga cttaaaatga aaatcatgat cgctaaactc agcagctgat
     3541 gaaagccaaa tacagccaag gcgattgtag tgagcaacgt gtttaaaaga gcgataacaa
     3601 attgagcttc taataccaaa ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat
     3661 cttgaaagaa ccaagaaaat tcacttttta aaaacaatct tgagaataca attgtttttt
     3721 tcttttcaat cataaaaaag aaacttaaga tgaatgacat agcaaacgcc actgccattt
     3781 ttcctatatc ctgtaaatag ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa
     3841 aatcgtttcg ctcaatataa gtatggatat attgtaaaac aggatctgca tcttttggct
     3901 gatgctcgta aaaatcaata acggactgga tcatatcaaa tgtttgatga ataagcatcg
     3961 ggatatactt tgtgaatgcc agatagataa ggtaaacgat cagtgtataa atcatgatag
     4021 ctaaaacacc tgtagggatt cggacaaatc gctgaatgat ttgaacaaga tgaatcatca
     4081 aataagtaaa gataaatgtg agcaaaatgg ttgtaatcat acttctaact aagaaaagaa
     4141 caaaaacgac aaataataaa acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct
     4201 gataaacggt caaatcgaca acctccaaat aacaaaaaat aaacacgtta ttcattatat
     4261 gatggaatat gaaaataaga aagagaaaga gcaaaaagaa aaaccgctaa atgacgttat
     4321 cttatgagtt ggttttcctc ataaaatcgt tcatctagca gtttcagccc tttttctggt
     4381 cttgatgaaa attatctagc atttttcggg tcagatgttg cagtgtatcc aattcttcct
     4441 gttcaagtgg aagataagac ttgatctttt gtggaatacc ttctgcttgt ttttgcaacg
     4501 cagcaccttg aggagtcagc gtaacattta cgattctttc atcacccgtt tcacgctgac
     4561 gattaatata atttttttct gctaactttt ttagtaaggg agtcagcgta ccagaatcca
     4621 gacacaaatc atttcctagc tctttcactg aagtcttttt cttttcccat aaaactaaca
     4681 tcacaagata ctgggtatag gtaagattca gcggcttgag gagggaagtg tagtgtctaa
     4741 tgatttcctt agaggcggca tataaaggaa aacatacttg attaactaaa tatagatctt
     4801 ccatttttcc acctccgctg ttttactata gtgtatcata gcggatttac acaaaaaagt
     4861 ctattcacta actgctttca gagtaacttc tatatttcca gctgtagctt ttgaatacgg
     4921 acacacttta tgcgctgcct ctaataattc ttgcgtttct tccagagaaa gaccttcgat
     4981 agatccttca atctctacac ctaagacaaa gctcgtttca ctttctgaat aaagggaaac
     5041 ttgtgcactg acagtagaag ccccagagat atttttttgc tgtttgatca attctaatgc
     5101 actgttaaaa catgcactga aaccagctgc aaacaattgt tcagggttgg ttgcattttc
     5161 gacgcgttta ccaggagctg caatattcaa ttcgaatgta cgatcgggat tatgaacttc
     5221 tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac atttttttca taaaattacc
     5281 acctttaatt atattttata caattgaatt ttacacaata taattgtgga aagcaaagaa
     5341 atggattggc ataagataaa taagagaaag cacatgaaac agacgcgctt tctcgaatag
     5401 caaacatata caaaaaaata tcagagacaa aaactaatct catcataaac attacatata
     5461 tttctcaaaa agttgttttc tttcttcaaa acttacttca cgatggacct gatgaagcat
     5521 ccataaataa ccaaaaggat ctctgaacat tgagttagtc acaccaaagt ccatcatttc
     5581 tgtcatttct tgaatctcta cacagccatt tcgtaatgct ttattatgtg tgtcctcgat
     5641 atcagagacg atgatattga accagatcgt atttggatgt tcagcagtag gagcagtcaa
     5701 accaagagac gtgttttcat ccagcatatg gaatctcgtg ccatacaagg taaaaatgac
     5761 ttcattattg cctctagggt aatctgttgc ctcaatcagt tgtatttcat caaagattgt
     5821 ttgatacagt tctagtgctt tcaaactatc atttaataca aaatctattt ccgttccttt
     5881 atgcataaag atctcctctc cttttcagtc gaacagttac tagattcttt tattatacca
     5941 gttaaagaaa acgcattcaa aaaaatagcg gatttactta ccaactcatt cgattggatg
     6001 ataagtaaat ccgcgtccca ttacttctgc tacgctgctg acaatgacaa aggcacttgg
     6061 atcgatttct tctatcaatg gtttcattcg ggaaaattca tgatcagtca caacgatcaa
     6121 taaaacctct tttgactttc gattataccc gccttttgcc tctagcaggg taaggccacg
     6181 tccaatctca gcacttaagc gaaggcggat gtcttccaaa taatgttcac tcatgaccat
     6241 aacagatttc tttcgattga tacctgtttc gatataagtc atgacaattg aagtgattgc
     6301 aatagataaa atagcataaa gaaactcttc tattccaaaa acaaacagat taagagaaac
     6361 aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc aagtggaaat attttttaaa
     6421 aatgagggga gggatcgtcg taccgccgct agaagagttg tttttatata aaatcgcaac
     6481 tcctaaagca aaaatcgcac ttccaaaaat gacagacaac aaccgatctg aagtaatcat
     6541 tgtttctgga actattgcaa tgattaaagg aaaaaacaag cttccgaaaa gtactttaaa
     6601 aaagggtttt ttacctaaaa atagaagagc tagtaccaac ataacgatat tcaacactaa
     6661 aataactata gctcggtcaa atcctaaaac agcttccagt aatattccta gaccgctgac
     6721 acctccagca gcaatatgat gaggccctaa aaacatattg atagcgactc ctaatatgaa
     6781 gagggcaaaa ataatcttaa tgtatgtcca tataaattgt ctcgtttctt tcatttatcc
     6841 acttccttaa ttcattaata atactcttat cttattataa cttacttttc accttaagtt
     6901 aactttaatt aataaaaaaa ttaggctagt ctaaattatt atgtttcatt cttctagaat
     6961 atacttctac gttaaaaact gttaaaaaac ttttacttta gaaaattgga gaaattgatc
     7021 gcttaagata aattcaggac actaaatagt acaggaagta gattaaaact tttaaaagtt
     7081 ttaagtctat ctatttggca agtttaaaaa ataaaaattc ggtatcaaaa aactgttacc
     7141 gataacattt ttgaaagcgt tgtcttttgt ttctctatca cttatagtga tgacgtcata
     7201 ttaaaacaca aggaggaaat caatggatac agctgcaatt tatcatcgac cggaaagtga
     7261 atatgcattt ctatatacga atacaagatt tcgtattcgg cttcgtacta ggaaaaatga
     7321 tatcaaaaaa gtatatgtgc tttgtggaga tccatatacc ataacaactg aaaaatggta
     7381 tcaaaaacaa cgaccaatga aaaaatgtct aagtaccaat gttcatgatt attgggaaat
     7441 tgaagtgacg agcgaaacaa ggcgcttaca gtatgcgttc catgttgttg gggaagataa
     7501 cacggactgt ttttacggtg atcaaggaat ttttccttat cgagaaaatg tgattactga
     7561 accaaatttt tattttcgaa taccttattt tcatcaaatc gatcgattca ctattccaga
     7621 atgggtaaaa gaaacagtat ggtatcaaat ttttcccgaa agatttgcaa acggggacaa
     7681 agcaaatgac cccgaacaca ctttaccttg gggaagtaaa gatccagacc gagaagactt
     7741 cttcggggga gatttgcaag gtgtgaggga tcatttggat tacttgacgg accttggcgt
     7801 caatggcatc tatttttgtc ccatatttaa agccacatcc aatcataaat acgatacgat
     7861 tgattattat gaggtggatc ccgcttttgg cgataaaaaa cttttaaaaa acttgattga
     7921 tgaagcacat aaaagaggga tacgtatcat gttcgatgct gtattcaatc atatgggtgt
     7981 ccattcacca cagtggcaag acgttctcaa aaacggagaa aaatcgatct ataaagactg
     8041 gttccacatc cgttttttcc cagtagattc ttatcagatg actgacttac cagaaactgc
     8101 tgaaaatatc ccatatgata cttttgcttt tactccattc atgccgaaac taaatacagc
     8161 aaatccagaa gtgcaaaaat atttgctgga tatcgcaact tattggataa aagaattcga
     8221 tattgatgga tggcgtcttg atgttgctaa tgaagtcgat catcattttt ggaaaaaatt
     8281 cagagaagca gttacagaaa tcaaaccaga catctatatc cttggagaga tttggcattc
     8341 ctctcaagct tggctgcaag gggatgaatt ccatgcagtg atgaattatg cattcacaga
     8401 ttcgattaaa gattattttg caaaaaagaa aatcacagct tcacaaatgg tcagtggaat
     8461 gaatcatcag caaatgcttt acagagatca ggtgaacgaa ggaacattca acttattaga
     8521 ttctcatgat actgccagga tcttgaccct ttgccaaggt aacaaagaat tgatgaagtc
     8581 tgtgatggcc tttatgtttc ttcaaaaagg gtctccttgt atctattacg ggacagaaat
     8641 agggatgact ggtgaagatg acccggattg ccgcaaatgt atgatatggg agaaggagga
     8701 acaagatcta gagctattcg gttttataaa agaacttgtt tctctaagga aacagttgtc
     8761 taagattatt tctgaaggtt ctactcagtg gctaatagta tatgatcgtg aagataagct
     8821 ctatttcaca cgggaactag agggacagat catctatgtt tactttaatc agtcaaaaga
     8881 accttgggta gtagaacagg aaaatgaggt gattcttagt caaaattgtc agttgttaga
     8941 agacgggaaa gcagagatac gacaatacgg atttttgatc atgtgttgaa aactgttacc
     9001 ggtaacaaca agaaagcgtt tgcaaatgaa gatttgtgcc cattataata agggtgtaac
     9061 agcacgttga atacacagga ggaaatgaag atgaaaaaaa gaagtgtgaa acagttgggt
     9121 ttagggttaa tctctttagg aatcttaggc ggattagcgg cttgtggcaa tggtaactct
     9181 tcagcagaag gggaagccga tgaaggaaaa acgttaacag tttccgttga ttctggatat
     9241 aaagattata taaacgaaat aaaaaacact ttcgaaaaag aaaacgatgt gaaaatcaag
     9301 ctagtagaaa aggacatgtt cgatcagttg gaatcattag cgttagatgg gcctgctgga
     9361 aagggacctg acgttatgat ggcagcatat gaccggatcg gtgcgttagg acagcaagga
     9421 catctggcgg aagtaaaact gggaaatgaa gcagcttacg atgaaacaga caaagcccaa
     9481 gtgacttatg atggaaaaat ctacggagaa cctgcagtaa tcgagaccct tgttttgtac
     9541 tataacaaag atttagtcga tacagcacca tcaacgttta aagatttgga gaatctatcc
     9601 aaagacagtc gttttgcttt tgagtcagaa gcaggaaaaa atacaggatt tttagctaaa
     9661 tggactgact tttattattc ctatggttta attgcaggtt acggtggcta tgtatttggt
     9721 gatgatggca caaatccttc agatatcggt ttgaataatg caggtgctgt agaaggaatc
     9781 tcttatgcaa cagattggtt caaaaatgtt tggccaaagg gaatgcaaga cataaaaagt
     9841 gcaggagact tcgccagcca gcaattcatg tccaataaaa ctgcagcaat cattgacggt
     9901 ccatggcaag cgcaaacata caaagaaaac aatatcaatt atggtgtagc caaaattcct
     9961 actctaaata atggacagcc ttatcaaccg tttggcggag gaaaaggatg ggtagtcagc
    10021 aattatgcta aaaacaaaga cctctctcaa aaatggcttg attatgtaac gaatcaggaa
    10081 aatcaagaaa aattctttga aatggtaaat gaaatccctg caaaccaaca agctagggaa
    10141 acagcaaagg ctaaaaacga tgaattaaca acagctgtga ttgaacaata tgaaacagct
    10201 caagcgatgc caaacatccc agaaatgggc gaagtttgga caggtgcaga aaacttgatg
    10261 ttcgatgcag catcaggttc aaaaacacca aaagaatccg cagatgaagc agtaaaaaca
    10321 atcagtgaag ccatagaaca aaaatactga ttaaaatgat aaccgtccat ttttataaaa
    10381 aatggaacag gtgcttcaga taaaagtggc aagagcgaga tggaaaatta tcttgctctt
    10441 gttgccatta tcttaaaagc agtttgcggt ttagcaagtt tagagggggc attcagatgc
    10501 agcaagataa aacaagcaat atcaacgaaa aaaaaatcac gctttgttca cttattccag
    10561 ggatggggca gtttgtaaat ggccaagctt ttaaaggtat cgtttttttc gccatattcg
    10621 tcctattcat tggtcagatg cttctgggcg gatttcatgc attggtagga ttggttactt
    10681 tgggaagtat accaatggaa gatcattcat tatttatttt gattgaaggc actttgcaat
    10741 tcatcattac aatcattttc ttgatttttt acggtttgaa tcttttagac gcttatcgag
    10801 tagcgaagat gcgtcaagag ggcaaaaaaa tcaatcggac gatgaaggaa gtcatacata
    10861 atataggtga tgaaggtttt ccttatctat tgacacttcc cgcttatctt ctcatgattt
    10921 ttacgatcat tttcccagtc ctagttacat tgttcactgc atttactaat tatgacttca
    10981 atcatattcc accagccagt ttaattgatt gggtaggggc taaaaacttt tttagtatct
    11041 tctttttaag ctcatatcgt aatacttttt ttgcagtctt ttcgtggacc gtgatttgga
    11101 ctttatgtgc aacaactttg caaatcaccc ttggtattct cacagcggtt gttgctcacc
    11161 agaaatttat taaattcaaa cgtattttcg gtatcatttt cttattacca tgggcggtcc
    11221 cagcttttat tactatcatg agtttttcga atatattcaa cgatagcgta ggtgccatca
    11281 atactcaagt gattcctttc ataaaccagc ttccttttgt agatatcagt gcaatctctt
    11341 ggaaaacgga cccgaactgg acgaaagtag caattatctt agtgcagggc tggttaggat
    11401 ttccctatat ctatgtgatg gttacaggaa tcttacaatc gatttcagaa gacttatatg
    11461 aagcagcaaa aatcgacgga gcaaatgcat ggcagcgctt ttcaaacatc acgcttcctg
    11521 ctgtcttcct agtggcagcg ccaacattta ttacacagta tacggggaat ttcaataatt
    11581 tctccatgat ctatctgttt aacaatggtg gtcccggaag cgtaggcgga ggagctggtt
    11641 ctactgatat tcttatttcc tggatctata aacttacgac tggaacttct ccgcagtatt
    11701 ctatggcttc agcaatcacg ttgattatct ccgtcatcgt tatctgtgtg tcattgctgg
    11761 tatttaaaaa gacaaatgcg ttcaatatgg gggattaaca atgaaaaaat ttcattcgag
    11821 tgctcgattc caaaaacgac tttctcactt ttttacttac ttttttcttt taatactgtc
    11881 agtcattatc atctatccgc ttttgatcac agcaagtact gccttcaagt caggaaatat
    11941 tgcagctttt agtttagatc tttcaagtga ctggacttta ggaaacttta ctcggctatt
    12001 cagtgaaaca ctttacggga cttggtataa aaatacattg atcatttctg tcctaacaat
    12061 gatcgtacaa gtgactgtag taactttggc gggctatact tatagtcgct atcgttttat
    12121 cggccgcaaa tcaagtttga tgttcttttt agtgattcag atggtaccga ctatggcagc
    12181 attgactgcc ttttatgtca tggctttctt attagaagcg ctcgataagt actggttctt
    12241 gacaatgatc tatataggcg gtggaattcc aatgaatact tggctgatga aaggatactt
    12301 tgacactgta ccaatagatt tagatgaatc tgctaaactt gatggggcag ggcatttcag
    12361 gatttttttc cagattgtat taccgcttgt caaaccgatg ctcgccgttc aagcattatg
    12421 ggcttttatg ggaccttttg gggatttcat gttagctaag ttcctgcttc gaacacctga
    12481 aaatcagact gttgctgtcg gcttacaaac atttatttct gatgtgacaa accaaagagt
    12541 ctctcttttt gctgctgggg caattttgat tgcactaccg atttgtctat tattcttctt
    12601 tttacaaaag aactttgtta ccggcctgac atctggcgga acaaaaggat aggagcagag
    12661 tatcatgcaa acatttccgt taaactattt ttccgcatta cgtacgccaa ctcaagtgtt
    12721 tgcgggacgc aaactattat catggcctaa atttttcttg atatttgtat ttttagtttc
    12781 cttgatggtc atgcctgtga ctctttttta tgccaatcag atccaagcga ttccattaga
    12841 acaatttcta tccgttcatt cgctgatcga tgaacaagga acccaaaaat tttctgaact
    12901 tgaattatct gaaactgggt tacaagcatc gcaacaaacg atagctgtga cccctgaaat
    12961 tttggtaggt gtgtctttat ctgagaaaca acaatccgat catggaactt ttattgattt
    13021 tgagaaggaa caatgggtga tccaacaaaa ggataaatct ggtatacgcc gctatacgat
    13081 gaattatagc ccatctttcc aaccagattc tgtacgtacg cccgaagatt tccaacgatt
    13141 tttagaaaga gaattttatg ccagtaatcg tccaacaatc attttatctt atagtttaag
    13201 tcttggtctt gtcctatttg tcatgacgag tctcatttta ttcggggctt cctttttctt
    13261 gtggatgaca agaaaaagcc aactttcatc cattcataca ttcaaagaga gtgcaaattt
    13321 gatgctgaat gttatgggaa taggttccat ggcagcagca gttgtagggt ttatccactt
    13381 tgactttatc ttgatgctaa gtgtccaaac aacgataact gtcttgctgc ttttatgggt
    13441 ttttgtcaaa acaagattca aagataagcg tgtcgaatga caagtttaag cgatttatgc
    13501 gacaatgaat gtgaaaaaaa tgaaaaataa agtagttttt gaaaaatttc tgaaaaaatt
    13561 aaacttctta gttgattctt ttctttttta gtattaacgt aatagggtag tttttttaag
    13621 gaggaaaatc atgttagaga aaactttata caatcaatta ttttcacgtt ctttctcgct
    13681 ccctgtcgaa gtaaattatt gggacggtac aacgaaacaa tacggagata cggacactcc
    13741 tccccaaatc aaaatcaata tccatgaaga aatcccaatg aaagagatta caagtaacgc
    13801 ttctttggca ctaggagaag cctatatgga tcatcgaatc gagatcgagg gaagtatcca
    13861 agcattgatc aacgatgctt atcagcaagc aaacagcttt ttacgaggaa aagattatct
    13921 aaaatggctg ccaaagaaag aaaaacatac gaaacaaaaa aacaaagaag atatccatgc
    13981 gcattatgac ttaggtaacg acttttataa actttggcta gatccaactt tgacttattc
    14041 ttgtgcttat ttcaatacac ctgaagatac gttggaacaa gcacaaatca ataaagtcca
    14101 tcatatctta gacaaactct ttatcaaaga aggcgaaacg ttgcttgata tcggttgtgg
    14161 ctggggaaca ttgatgttca ctgctaccaa agagtataat gtcaaggcaa ctgggatcac
    14221 actaagtgaa gaacaatacg atttgattac caaaagaata aaagaagaac atttagaaga
    14281 taaatgccgt gtattattaa tggattaccg cgaactaaaa ggcgaaacgt ttgatcatat
    14341 cacaagtgta ggtatgttcg aacatgtagg ttctgaaaat cttgaaggtt atttcaaagt
    14401 ggtaaaagat ttattgaaac caaaaggaac agctttgatc catggaatca gccgacaaca
    14461 aggcggtgcg acaaatgctt ggatcaataa atatattttc cctggcggtt atgtccctgg
    14521 tgttactgaa ttagttgata atattacaaa aaacgatttg caactgattg atttggaaag
    14581 cttacgtcgt gactaccaat tgacattaga acattggaca aagaattttc atcaagtcca
    14641 agatcaagtc acgaaagaaa tgggtgattc cttctatcga atgtgggatc tatatttaca
    14701 agcttgtgca gcttcatttg aatcaagtaa tatcgatgtg attcaatatt tgcttgttca
    14761 acctggaaac aatgacatcc caatgcatcg tgcataattg aggcaaaagt actgtttaaa
    14821 gactatttaa aaagaagcta agttttatga tgctccagaa gtgaactttt ttgtctaact
    14881 tctaggattg
//
LOCUS       DACCXM010000063        14820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCXM010000063 DACCXM010000000
VERSION     DACCXM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.065"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(296..1024)
                     /locus_tag="IVY61_001714"
     CDS             complement(296..1024)
                     /locus_tag="IVY61_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7970828.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IVY61_001715"
     CDS             complement(1002..2576)
                     /locus_tag="IVY61_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7970829.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IVY61_001716"
     CDS             2805..3743
                     /locus_tag="IVY61_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7970830.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IVY61_001717"
     CDS             3755..4666
                     /locus_tag="IVY61_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7970831.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IVY61_001718"
     CDS             4690..6162
                     /locus_tag="IVY61_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7970832.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="IVY61_001719"
     CDS             6213..6824
                     /locus_tag="IVY61_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7970833.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="IVY61_001720"
     CDS             6854..8767
                     /locus_tag="IVY61_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP7970834.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="IVY61_001721"
     CDS             8895..11876
                     /locus_tag="IVY61_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP7970835.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="IVY61_001722"
     CDS             11873..13534
                     /locus_tag="IVY61_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP7970836.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="IVY61_001723"
     CDS             13574..14506
                     /locus_tag="IVY61_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP7970837.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4818 a         2333 c         2979 g         4690 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
//
LOCUS       DACCXM010000064        14797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCXM010000064 DACCXM010000000
VERSION     DACCXM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.066"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            50..1789
                     /gene="yfcC"
                     /locus_tag="IVY61_001724"
     CDS             50..1789
                     /gene="yfcC"
                     /locus_tag="IVY61_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7970838.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1946..3130
                     /gene="iadA"
                     /locus_tag="IVY61_001725"
     CDS             1946..3130
                     /gene="iadA"
                     /locus_tag="IVY61_001725"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP7970839.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3241..3444
                     /locus_tag="IVY61_001726"
     CDS             3241..3444
                     /locus_tag="IVY61_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7970840.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3545..4207)
                     /locus_tag="IVY61_001727"
     CDS             complement(3545..4207)
                     /locus_tag="IVY61_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970841.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4220..5722)
                     /locus_tag="IVY61_001728"
     CDS             complement(4220..5722)
                     /locus_tag="IVY61_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7970842.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6270..7949
                     /locus_tag="IVY61_001729"
     CDS             6270..7949
                     /locus_tag="IVY61_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7970843.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8076..8555
                     /locus_tag="IVY61_001730"
     CDS             8076..8555
                     /locus_tag="IVY61_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP7970844.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8774..9454
                     /locus_tag="IVY61_001731"
     CDS             8774..9454
                     /locus_tag="IVY61_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP7970845.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9462..10793
                     /locus_tag="IVY61_001732"
     CDS             9462..10793
                     /locus_tag="IVY61_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7970846.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10808..11770
                     /locus_tag="IVY61_001733"
     CDS             10808..11770
                     /locus_tag="IVY61_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP7970847.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11881..12219
                     /locus_tag="IVY61_001734"
     CDS             11881..12219
                     /locus_tag="IVY61_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP7970848.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12298..13005
                     /locus_tag="IVY61_001735"
     CDS             12298..13005
                     /locus_tag="IVY61_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP7970849.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13108..14589)
                     /locus_tag="IVY61_001736"
     CDS             complement(13108..14589)
                     /locus_tag="IVY61_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7970850.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4684 a         2548 c         3174 g         4391 t
ORIGIN      
        1 ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt tgaagaaaac
       61 aaattcttca ggaaaaccga aacgcgactt cactaaatta tcgacacccc atacgtatgt
      121 cattattttt ggtgtagtca tctttgcatg gatactgact tttgtcgttc cagcaggaaa
      181 gttcagcacg caagatattg aatacaaaga tgcaaatggc gaaacaagta ctcgtactgt
      241 tttacgtcaa gattccttcc gttatgctta cgagctggat aagtcgtatg tgtttgatca
      301 attagaagaa ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc cggaaaaagg
      361 attggaaaaa gtcattgctg acggagaaaa aaatcttacg caggaaaaac tagatgaaat
      421 ctccttgaca gatgatgtgc tttacgatca gtacggcgaa aatatctatg atacttctaa
      481 aaaattgcat aaaacagcca aaatatgggg aacagatgat tttggcggct ttggtttctt
      541 aaattttgtt tttgaaggac tagtatctgg agataagtat ggatctgctg tagggatcgc
      601 tgcattgatc cttgttgttg gaggtgcttt tggtattatt atgcgtaccg gagcgatcga
      661 cgcaggtatc tatgcgttta tcagtaaaac taagggactg gagcgtttag ctttaccgct
      721 attattcttt gctttttcat tcggtggcgc tacattcggg atggcagaag aggtaattcc
      781 attctcaatg gtgatggttc catttgtcat tgcgttagga tatgactcga tcgtagcagt
      841 aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga gcccgtttag
      901 cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg tctggtgcga cattccgatt
      961 gattatgtgg gtagttgtta ctgcattagc cgctggttat atgatgatat atgcagaaaa
     1021 aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca gatgattatt tccgttctca
     1081 tatcaaaaag acatctgatg aaaataaaaa attcatgctt gggcataaat tgatcttatt
     1141 agagatgtta gttgtccttg tctggatcgt ttggggcgtg actcaaaaag gctattacat
     1201 tccagaaatc gcttctcaat tctttgtgat gggattagtt gctggaatca ttgctgttct
     1261 tttcaaatta gatggaatga aagtcaatga catcgcttct tctttccaat ctggtgcagc
     1321 tgatttagct ggtacagcaa tcgtagtagg tatggccaaa gggattcttc ttgtcttggg
     1381 aggatctgat gcatctgttc cttcagcttt gaatacgatt ttgcatggga tcgggacggc
     1441 gttgaccggt gttccagctg tcattggtgc ttgggcgatg tacatcttcc aaagtttgtt
     1501 caacttggtt gttacatcaa attcaggaca agcagctttg actatgccaa tcatggctcc
     1561 actggcagat ttagtaggtg tatccagaca agtagcagtt cttgcttatc agcttggttc
     1621 aggttttatg gacgctttta cacccgtatc agccagcttg attggtgtgc taggtgttgc
     1681 acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat tcctgtttgt
     1741 tttaggaact attttcatca tgattgccat tgcaataggt ctgcagtagt actggagacc
     1801 tgtaaataaa aaagtaatcc aaacagtaga taaggactcc aagaaccgtc attttacggt
     1861 aaaatcaaag gagtaaagat cctatcgttt ggattatttt ttaagatcct atcatcagtt
     1921 atacttaaag aaagaggaaa aatgtatgaa aatcattcga caaattgaag tgtttgcacc
     1981 agattactta ggaaaaatgg atgtactgat tgccggtggg aagattttgg ctgtagaaga
     2041 acagctagaa ggcggctacg aaggagtgga agtagaagaa ctttcaggtg agggaaaagt
     2101 actaaccccg ggtttcattg actgtcattt ccatatttta ggtggaggag gagaaggcgg
     2161 ttatcaaaat cgaacgccag aagtcacatt gagccaattg actacagctg gtgtaacgac
     2221 agttgtcggt tgtttgggaa cagacggaga aggtagagat atgactgcgt tgatcagcaa
     2281 agcgaaaggt ctggaagcag aaggaatctc cacttatgta tacgaaggtt cgtaccgatt
     2341 gccggtaaaa acagtaacag attctatcat caaagacttt ttgacaattg ataaaattat
     2401 cggaatcgga gaaatcgcag tatccgatca ccgttcttct cagccaagtt ttgaagagtt
     2461 cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag ctgggatcat
     2521 caatgtccac ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa tcgtagaaga
     2581 aacagaaatt ccgatcacac aatttttgcc tactcatgca aatcgcacac cagaattatt
     2641 tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt gacttcactg ctagcgaaga
     2701 tcctgatttt tgggagaaga cagatggtga agttcgcttc agcaaagcac tgaaacgatt
     2761 gatcgaggaa gatgtttctt tagataattt cacgatgacc tcagatggac aaggaagctt
     2821 gccttatttt gatgaaaaca atcatttcct agggttagga gtaggaagtg ccaaggcatt
     2881 gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa tcgccttacg
     2941 agctataacc agtaatccag cacgtatatt gaaattggat aaaaaaggaa aaatcgaaat
     3001 tggtgctgat gcagatctgt gtatcttgga taaagaaacg cttgatattg atacagttat
     3061 cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa gtatggggga ctttcgaaaa
     3121 aagtttttaa tcaacagctc taactatctt ttttagaaaa cgagcgttct gtttgtctgt
     3181 atctgttgtt gatgttaccc tgaaggtaga tcaataaaca aactaagagg aggaagcaca
     3241 atggcagatt taaaaggacg ttctaaagat atgaaagata aagtagtcgg tgaaggaaaa
     3301 gaagcatacg gcaaagcaac agacgacaag agcaaagaaa cagaagggaa agcacaatca
     3361 ctaggtgctg acatcaaaga aaaagctcga aatttaggcg acgaagtcaa agacaagttt
     3421 tctcgaaaag acgaaaaaaa ataaaaacca cttattacta attgaatcaa gcaaataaaa
     3481 aatccgaacc atatctgcga gacagatatg gttcggattt tttatttgat cattatcctg
     3541 ttttttaaac ttcgattttt tgaaattttt tattttttag ttcaaagact tcatcacagc
     3601 tttttgctac ttcaatctca tgagtcacga tgataataca gcgattttgt tcatgggcga
     3661 tttcttggaa aagtttgacg atatcttgtg aagttgtttc atctagattt ccagtaggct
     3721 catccgctac gattagttga tgttcacagc agatagcacg aacgatagcg actcgctgct
     3781 gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa tatttcatca attcctacac
     3841 gagccagtcg atctaaagca acttgtcgat aattctcttg cttggtctta gtgatttcca
     3901 ttgctgctat gacgttgtct agagccgata aataaggaag caagttgtaa gcttggaaaa
     3961 taatcgagac atcattttta cgataacttc ttaacccgat tttgttcagt gaacgatctt
     4021 gatagtatac ctttccttct tttggtgcat ctagtccaga aataagcgaa agaaaagtcg
     4081 tttttccaga accacttgct cctaaaatag cgtacatttt tccagcttcg aaattcagtt
     4141 ctacatcttt ataaagcgca tcttgcggat cttgatacca aaatcccaat tgttctgtcc
     4201 gtaacatcaa attcctcctt tagtttaata ggatttttct tgggtttaat cttaatatcc
     4261 ctatagaagc taaaaagaca gaaaacagac tgattccaaa tgctactccg gctaatagtc
     4321 ccaattgtgc aggtttgaca ttgatttcca attcgctgac ttgatcagaa acagcaaatg
     4381 gattattcgt ggttccttgt tgaccatcgc gttgaccgcc ttgttcttgt ctgttacctg
     4441 gcatttgtcc accgccagga gcttgctggt tttgtgtcgt ggtttctgtc tgttggtcaa
     4501 gtagctgctg tcccacggca ttcgcgacga cgtttccgct aagcgaagca actcctaatg
     4561 caagaatcat acaaatagcc acttcggtaa acaattggag aatgatttta cttctggatt
     4621 ctcctaaaga tagcaagacg cctaactcat gccgacgctc ccgaatggta atcataatga
     4681 tcagagtaag tatgataata ccggctacag ccactagtaa aacaacattt ttagaaaaac
     4741 ttgccacgtt gtttaaaggt tccaacattg actgatacat ggaatcattt gattgtaagc
     4801 taaatgtatc tgtgtcgatc agcttctcag cttctttgag aaactcatcc attttatctg
     4861 gatcagatag agtgtaaata gcagaatcta tcgtgttatc gtcagatgtc ccatttagtt
     4921 catttgcaaa agtgtaagaa gtgtaaagtg tattcgacgg attcatgaag ttgaatttca
     4981 tacccatact gccgcttgtt tcgctggatt cataaattcc tttgatcgtc atttcataag
     5041 tggtatcttc atcttcagta ctcgtgattg tgaaagtatc tccgactgcc aggtcatttg
     5101 tttctgctag agtagaatca atgactacat tgtttgtgcc tttgtcgctg tcatctattc
     5161 cttcaccatc cgtgattttt gctgttccat cagaaaaatt actattttgt gcagttgctg
     5221 acacaccact gatttgaaag tctgcttgag tcatttgcgg attcccgccc attcctccag
     5281 caggttcacc gcctgcatca gtagtttctg aactgtcttc acttgaatca gaactagtga
     5341 tggcagttat tccatctttt gccatagctg aggcggaact ttcaaaggaa taactcttta
     5401 cattatctaa ttcagctatt ttttccgcgt ccgttaagga aactggtgta agagaaaaac
     5461 tgcctggatc tggacgttct tcagtggtag aaccgcttga agaagtttgt tcctgttttt
     5521 taaatgaagc ttctcgattg gcacttaaag tcactgttgc tccaacttct ttttgtgctt
     5581 gattcgctgc ttcttctgca gcgcttcgaa tcgtgaggcc agctagcaca aaaataagaa
     5641 tggctgtgaa cacggcaaac aataacaatg aacgtcccca ttttactttt gtacttttcc
     5701 atgcacgttt cataaaattc atattttgca tccctcctag tagatgcatt tgataataga
     5761 caacctatct taaagctagc ttaaatctta tgctgtttgc acaccatcaa catgaggttc
     5821 tgtatatata aaaagtcact atagttattg caaaaaatgt ggtaaaatga aaaaagtatg
     5881 tttcaaaccg acgaagaaga aatgaaagag gaatagcaaa agaaatccta gatgctacag
     5941 agggattttt acgtgcatag ttgttctaac aattgctgat actagcgatt tagtttattt
     6001 gttgtataat aatggggaac agctgaatac ttatataagt attctggcta tagcgtttcg
     6061 caaaagatcc attttgttaa tgaagtatat ccaaaataaa atgcccatcc aactgtcaga
     6121 acgcggatgt gctggtaatt ttcctgctga ttctccataa gttgagaaat agtgattttg
     6181 catatgcttc attggaatgg ctcatatatt tagataaaga agaaaatcga catctttaaa
     6241 cttggatgtc ataagaagag gtgaagtttg tgagtaccat taaaattatc ccattaggcg
     6301 gcgttcgtga aaatggaaaa aacatgtatg tcgcagaagt gggcgaggat atttttattt
     6361 tagattgtgg gctaaaatat cctgaaaatg agttgttggg gatcgacgtc gtaatccctg
     6421 attttacgta tttagaagaa aacattgatc gtgtagctgg tgtcttttta acacatggac
     6481 atgcagatgc gatcggggca cttccttact tgttgtcaaa aatttctgta ccagtattcg
     6541 ggacagagtt gacgatcgag ttagcaaaat tgaatgtcag tcgtaatgaa gcagccaaag
     6601 gatttaagga tttccatgtg gtagatgagc atacagaaat agactttggt gaaacagtag
     6661 tgagcttttt ccgtaccact catacgatac cagattcagt ggggatcaat ctgaaaacaa
     6721 aagaaggtag tatcgtttat acgggagatt ttaaatttga ccaaacggct atcccaatgt
     6781 atcaaacaga ttatgcacga ttggcggaaa tcggcaaaga aggagtctta gccttactaa
     6841 gtgaatcggc aaatgcagaa aatcctcagc ctgtagcttc tgaagcccaa atagcagatg
     6901 aagtatttga tacgatcaaa tattgggaag ggcgtatcat cgtcgcttgt gtagcaagta
     6961 atttgcaacg agtgcaacaa gtattggatg cagcagaaaa agcacaacga aaagttgtct
     7021 tgactggtca agatttcgaa cgaattattc gcacagcgat gcgtttagaa aaacttcaat
     7081 taccaagtga agatctgctt gttacgttga aagacatgaa aaactatgat ccagaagaac
     7141 tgatcatttt agaaactggc cgaatgggtg aacccatcaa gtctctgcaa aaaatggcaa
     7201 acggtacaca ccgtaatgtc aagattgaag aaggagattt ggtttatatc actactacgc
     7261 caagcatcgc gatggaaacc atcgtggcga aaacggaaga tatcatttat cgagctggtg
     7321 gaacagtgaa gttgatctct gagaatatgc gcgtttctgg ccatgccaat cctaatgact
     7381 tgcaattaat gatcaatttg atgaaaccaa catattttgt gccagttcaa ggagagtatc
     7441 gtgaattagc tgctcatgca gatttggctc atgcagtagg gatgccatac aaaaatatct
     7501 atattactgg acgcggagac gtgctggaat ataagaacaa gcgtatgagt gttgcaggaa
     7561 ctacaagtgc agaaaatgta atgattgatg gattaggcat aggtgatatc ggaaacattg
     7621 ttttacgtga tcgaaaaatc ttgtcagaag acgggatttt cgtagcggtt gtcactatca
     7681 gtcggcgaga aaagaaaatc atttcaaaac ctcaaattac ttcacgggga ttcgtttacg
     7741 taaaagctag ccgtgatctg atgaaagaaa gcagcaatat gattgaaaca atcgttgaga
     7801 aacatttaga aagcaatgat tttgagtgga gcaaattaaa gcaagacatc cgagatcaat
     7861 taggccgtta cttatttgaa caaacaaaac gccgcccagt gatcttgcca gtcattatgg
     7921 aagctactca acgtcgaaat aaaaaataaa caaaaagagt aagatgaacc tctctataga
     7981 gtgctcgtct tgctcttttt ttgcgaaaaa caaataattt ttgaatacag catccctcgc
     8041 tttataataa acgatagaat aaagggagtg aatcaatgat gagtcaaaca aaagtcgaac
     8101 gttatttaga agaacaaaag atcagctatc aaccacttga ttttagtcat gtggtagaag
     8161 aatctttcac agaagaacta caaaaaagag ggattgatcc agctttgata tgtaaaactc
     8221 tcgtagtgaa aggaaacaaa actggggtga tcatcgcatt gattcctttg aacgatcact
     8281 tagattacaa aaaaacgaga caagtgacga aagatcgtaa agttggtttt cctggaatgg
     8341 attttgtatt aatgcataca ggatatcccc acggagcaaa cacccctatt ggaataaaac
     8401 tcgcacatcc tgattattgt tttttagcag acagttcttt aaaagaaaaa gaagaagtaa
     8461 ttgtgtcaag cggtgaaatc gggagaagcc ttcggataaa agtagccgat ttagaaaaga
     8521 tcgtccatcc tatttacgct gatttaatta gttaagggac gagaagagaa gctaagcaga
     8581 gcagagacct caaacttgag attatatctg cttggtactt ggtgcatcct gtattatctg
     8641 ttacaattgc tttgtacata ctgtactatc aatccaatga aaatatattt agcagctata
     8701 ttttttcatt gatgtgcaag tgatgaaatc gtttttaata aaaaagatac agagaaaacg
     8761 cgaggtgtaa aatatgaaaa ttacgtatca tggacattcc tgtttaacaa ttgaaatgga
     8821 aaatggccaa cgcgtgatga ttgacccgtt tattacagga aatccattga cagacttaac
     8881 tgcagaagaa gtaaagacag attggttgct gattactcat ggtcatagtg accacatagg
     8941 agatatgatt cctattgcaa aagaaaatca agccacaatt atcagtattg ttgagattgc
     9001 tgattatgcg aacagcagag gagtagattc attcggtatg aatatcggtg gaaaacatgc
     9061 gtttccattc ggcgtggtaa aattcgtaca tgcacagcat agttcaagct atgaagtaga
     9121 cggtatcgtt cagtatatgg gcgagcctag cggaattatt attcaagcag aaggaaaaac
     9181 gatttatcat gcaggggata ctgcttattt cagtgatcta ggattgcttg cagaagaatt
     9241 cgatattgat gtcgctttct tgccgattgg cgataactat acgatggggc cagaagatgc
     9301 agcaaaggct gcgcaaacaa ttcgtgccaa gaaagtagtt ccaatccatt acaatacttt
     9361 ccctgtcatt gaacaagatc ccgaatcttt tgtagagtta ctggaagata gtgaaggaaa
     9421 aataatgagc gtctcggaaa cgctggaagt gtaggcagaa aatggcgaca aaacatgatt
     9481 tgattttaga atatattgaa agtttacctg ttggcaatcg aatttctgta agaagcatcg
     9541 caaaagaatt gaatgtcagt gaaggaacag cgtaccgtgc aatcaaagat gcagaaaatg
     9601 tcggactagt ttcaacaatt caacgagtag ggaccatccg tatcgaacgc aaattaaaaa
     9661 aacatattga acgtttgaca tttggcgagg tcgtcaggat cattgaaggg gacgttttag
     9721 gcggggcggc aggattagac aaggtcttga ataaattcgt catcggcgcg atgactaaac
     9781 atgccatgga acgctatatt acgccaggat cactaatgat cgtcggcaac cgggcggaag
     9841 tccaaaaatt agcattggaa gatggcgccg cagtattgat cacaggagga ttcaatacga
     9901 ctgctgagat tgcaaagcta gctgatgatc ttgaaatgcc gatcttacgc acctcttatg
     9961 atacatttac agtcgcaacc atgatcaacc gagcgttaag cgaccaattg atcaaaaaag
    10021 atatcatgct tgtcagtgat atctatatgc cagctgaaaa gactcattat ttgcagcaga
    10081 cagatacgat ttctgattat cagcgtttgt cagaagaaac gcagcattcg cgttttccag
    10141 tcgtcaaccg ccatcatcgt ttgatgggga ttgttacggc aaaagatgtt ttagggaaat
    10201 cgccaaacca gatcatcgat cgggtgatga cgaaagaacc aatcagtgta aagaaaacaa
    10261 tgagtattgc ctctgttagc catcaaatga tctgggatgg gctagaagtc atgccggtcg
    10321 tttccgatga cttgacattg gaaggcttga ttacccggca agatgtaatg aaagcgatgc
    10381 agcttgtcca gcgacaaccg cagattgcag atacgatttc ggatcagata tctggaaata
    10441 tccatacgat tgatactgac cgtgaaggaa atcgtttgga aaaccctaag ttcaaattcg
    10501 tagtagcacc acaaatggtc aatggcgttg gaaccatttc ttttggcgta cttagcgaaa
    10561 tcatttctga tgtcgcacaa aaaacaatgg tgatgaacca aaaacgaaat atcttgattg
    10621 aacaagtgaa cctacattat ttgcggctga tccaattgga aagcgaatta gacattcgtc
    10681 cacgtgtatt ggaaatcggc agacgttccg ctaaactgga tatcgaagtg tttattgaaa
    10741 acaccatcgt ttccaaagca atcgttgttt gtcaagtaat ggaacgttct tagaaagcag
    10801 gagtgaaatg acagtacagc aagaaatagt agatacaata aaaaaatacg ataaaatcat
    10861 tatccacagg catttgcgcc ctgatcctga tgcattagga tcacaagtag gattagccga
    10921 actattacgg gaaagtttcc cagaaaaaga aatacgtcaa gtaggtgaaa cgattgaagg
    10981 gcttcgcttt ttagcagaaa tgcaagaagt tgaagatgtc ttctaccaag gagcgctagt
    11041 catcgtgact gatacagcta attcacccag aatcagtgat caaagatatc agttggcaga
    11101 aaaactgatc aaaatcgacc atcatcccaa tgatgaccct tatggagatc ttgtgtgggt
    11161 aaatacaaaa gcaagtagct gtagtgagat gattgcagaa tttgctttga tgtttcctga
    11221 atctttaaag atgaacgtta aggctgcacg gttactttat gcaggaatcg tcggagatac
    11281 agggcgcttt ttgtatccct ccacgacgtc ccgtacatta gagattgctt cgatattgag
    11341 aaattatcca tttgatgcaa gtgctttgaa cagagagatt gaacaaatgc cgatgaaagt
    11401 cgctaaactt tccggttatc tttatcaaaa tatccaggtg gatgaaaatg gtgcaggaag
    11461 aataatcctt ccgcaagaaa ttcttgataa atatggaatc gatgattcag agacagcttc
    11521 gatcgtttca cttcctggag tgattgacga agtgctggct tgggggattt ttgttcagca
    11581 gccttcaggg tattaccgcg tacgtttacg ttctaagggg ccgatcatca atgaattggc
    11641 taaacgccat catggaggag gccatccatt agcaagtggt gcgaatgcaa aagatttagc
    11701 agaagtagag gaaatctata aagagatcca agatatatgc caatcgtata agagcagaaa
    11761 aacagaatga tacttcgttc gaaaacgtgc cttttgtagt aaaaatcaca aatgtatctt
    11821 ttcaagatct tctttttccg ttacaatagt gctcactaaa gattttaaag gagaaagaag
    11881 atgacattac caaattgccc agaatgtggc tcagagtaca catatgaaga tcgaggcttg
    11941 ttgatttgcc cagaatgcgg gaacgaatgg aacaaagaag aacaagaaac tgtcatagaa
    12001 gacggattag tggtattaga tgcaaatgga aaccgtctgt tagagggcga tagcgtgaca
    12061 gtgattaaag acttgaaagt aaaaggtgcg tcaggtgcca ttaaacaagg aacaaaagtc
    12121 aagaacatcc gtttagtaga aggcgatcat aatatcgatt gcaaggtaga aggatttgga
    12181 ccgatgaaac tgaaatcgga atttgtgaaa aagaattaaa gaatattcaa ggtcttttca
    12241 cattcttttc ctactactaa tgataaaata aagtaaacga aaaggagtga aacgaaaatg
    12301 tatggttatg gaatgtatgg ttttttagat ccgacatttc ttttggtcat tcttggatta
    12361 gttatttccg gaattgcgtc agcgtacgtc aatagtactt tccgaaaata cgatcaagtg
    12421 agaagcaaaa acaatgtgac tggtacacaa gcagcacaat atatattaca aagtcaacag
    12481 ataaataatg tcggtgttca acaaattgcc ggagatctca cagataacta caattcaggt
    12541 aataagatgt tgagtttgtc tcaagcaact gcacagtcta cttcagttgc tgcgataggt
    12601 gtagcagctc atgaatgcgg acatgcggta caagatgctt cgggttatgt cccattgaag
    12661 attcgtgcag cacttgttcc agcagctaac tttggttcga tgatttcgtt ccccttgatt
    12721 atggtaggtg tattgcttag ctggaactct acgttaatca atatcgggat tttcgctttt
    12781 tctttagcat tacttttcca attggtgaca ttaccagtcg aatttaacgc gtctcggcga
    12841 gcaatccaaa ttttgagtga aggcggacta ctgacggaag aagaagtacc gatggcaaga
    12901 cacgtattat ttgcagcagc actgacttat gttgcagcag cgctttcgac attcctgcag
    12961 ctgttgcgct tgatcttatt gttcggcggc aaccgaagag attaaaataa agaagttgtg
    13021 aaatcagcgc tttgatctga gctaataccg agagttagct gattgaatac catttatttg
    13081 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
    13141 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
    13201 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
    13261 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
    13321 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
    13381 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
    13441 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
    13501 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
    13561 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
    13621 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
    13681 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
    13741 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
    13801 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
    13861 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
    13921 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
    13981 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
    14041 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
    14101 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
    14161 gatccgtaaa tggaatttta ttaaggaatg attgagctgt tttgtcagtt tttcgagata
    14221 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
    14281 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
    14341 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
    14401 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
    14461 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
    14521 agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt ttcttgcttc
    14581 aattagcata aatacctcct ttttattctg tttttgtata tagttagaca aaaactgtga
    14641 gtggaaagtc atttcagtct gtgttattct cacagagtgt cgccttcttt tttatataac
    14701 gttatttaat ttttttgtta ccttgtttat tctattgttt atttcctagg gtattttgtc
    14761 aagatattct tgtttttaag gcggaaaaat tgatttt
//
LOCUS       DACCXM010000065        14603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCXM010000065 DACCXM010000000
VERSION     DACCXM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.067"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(192..758)
                     /locus_tag="IVY61_001737"
     CDS             complement(192..758)
                     /locus_tag="IVY61_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7970851.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(758..1714)
                     /locus_tag="IVY61_001738"
     CDS             complement(758..1714)
                     /locus_tag="IVY61_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP7970852.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1714..2370)
                     /locus_tag="IVY61_001739"
     CDS             complement(1714..2370)
                     /locus_tag="IVY61_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7970853.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2740..4758
                     /locus_tag="IVY61_001740"
     CDS             2740..4758
                     /locus_tag="IVY61_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7970854.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5185..7599
                     /locus_tag="IVY61_001741"
     CDS             5185..7599
                     /locus_tag="IVY61_001741"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP7970855.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7947..9500
                     /locus_tag="IVY61_001742"
     CDS             7947..9500
                     /locus_tag="IVY61_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7970856.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9881..11665)
                     /locus_tag="IVY61_001743"
     CDS             complement(9881..11665)
                     /locus_tag="IVY61_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970857.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11680..13401)
                     /locus_tag="IVY61_001744"
     CDS             complement(11680..13401)
                     /locus_tag="IVY61_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970858.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13501..14262
                     /locus_tag="IVY61_001745"
     CDS             13501..14262
                     /locus_tag="IVY61_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7970859.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4740 a         2702 c         2912 g         4249 t
ORIGIN      
        1 aagctgtcct gcatcaagta ataagaacag ccaatcaaaa acagcttctc atgtttttga
       61 tttggttgga cttattaccg cagatataag cttttgagca ccgtgaataa agaggttatg
      121 aaatcaacgc tctaacctga gtattagaga aaaaaacgca aaacatatca tttatgtttt
      181 gcgtttttta tttatctttt tttctcaata caatataaaa taggcggctg gttcttttgg
      241 ttgataaacc gataattcag cacattatat gtttcctgtg gcagtttgga acaaaaagaa
      301 tcaaccatat ccagttcttt ttctccacct tcatgaccat agtagacgac aaggatcaat
      361 cgtccgcgag gcacaagtcg tttcaatatt tcttccatag ctgtgcgtgt agtctctggc
      421 atagtgatga ttgctttatc actttttggg agatacccta gattgaaaat ccctgctttg
      481 agattttgtt gttcagctat cacttctcct aacgtttcgt ggccttgtag aaacaatgag
      541 acacgttctt ctaactgacg ctgttctagc ctttcacgag tagcatcgat tgcttgttgc
      601 tggatatcaa aactatagac atgaccagtt tttccgatgt gttctgctag aaaaaccgta
      661 tcgtggccat tgcccatcgt tgcatctacg acgtgatccc ctggttgcaa gatttcttgc
      721 agtaatgtat ggctgaaatg caaagcagtt tgcaacatca gacgctcacc tttttccgta
      781 catcatattt tccttggaag ctatctctgc gaagaagttc ttcatcaatt gcattcaaga
      841 cttcccattt tttcaagctc cacattggtc caatcaagga atcccacggc gcatcccctg
      901 tcaaacgatg gatgatgatc tcttttggta tcatttccag ctggtcgcaa atgacattga
      961 catagtcagg acggctcatt aattgaaggc gaccttcatg atagtcccgc aacattctcg
     1021 ttttgcgcat cagatgcatc aaatggagct tgattccttg gatatctgaa tctaaaatgg
     1081 tgcgtctgac attttctttc atcatttcta aactttcgcc tggtaatccg ttgatcaaat
     1141 gcgtacaaac acggatatta tgtttgcgta atttagccac gccatctaaa taagtttgat
     1201 aatcatgtgc tcggttgatc agttttgaag tttcttcaaa agttgtttgc aatccaagtt
     1261 ctacccataa atacatccgc tcgttcagtt cagcaaggta atcaactact tcatctggca
     1321 gacaatccgg tcgtgtaccg actgataatc ctacgacacc tggtaaattg actacttgct
     1381 caaatcgttc tttgattatt tcaactggcg catgggtgtt tgtaaaattt tggaaataaa
     1441 cgatgtattg ttggacatga ggccattttt gatgcatcat gtcgatttct ttctggaatt
     1501 ggattggaag agggtcttca ggtgcaacga tcatgtctcc cgaaccagat acactgcaaa
     1561 aagtacaacc gccatgggca acagtcccat ctcgattcgg acagtcaaat cctccatcga
     1621 tcggcacctt gaaaattttc ccgccaaact catttcgtaa agcgtaattc caagaatgat
     1681 atcttttatt tccttctgaa taaggaaaag tcattaaaag tccttctttc tgtaagagtt
     1741 atctatttgt tttctttttt ctctatacac tggataagtc aggcacagcc aagaaagact
     1801 gagtaagtaa ccgccgatga tatcagtagg aaaatgaacg cccagataaa tgcggcttat
     1861 cccgacactt actataataa acatcatcag caattgaagc aatgtgcgta aggttctttt
     1921 ttgaacaaaa gctggaagca gaaatagtaa agaaccgtaa agaatcatac tgcctgctgc
     1981 atgtccacta gggaaactgt aacttgtttc aactactaag tgcgccaatg aaggacgctc
     2041 tcgcatgaaa aatagtttga tcaccgggtt gatgattcca gcaattacaa taaatccaga
     2101 aacgaaccaa aatgtttctg cccttttttt cttataccac atccataaag ccgcacaagc
     2161 aaataaaatg atgacggtaa gaggattacc gaatttagtc ataaagagtt ggtagctgtt
     2221 cagatcagga taagaagctc tgacaatgcc tgtaaaccac tgatcgaaac cagaaagcca
     2281 cgacagatgg aactttacga tccagcccaa aatgaaaaac aggacaagaa agaagctacc
     2341 agccacctga aatgatgttt tatatttcat acaattcctc ctttgaagca atgacttatt
     2401 atagcatttt tggtccagcg aaaaaaacgg taaagagata aaaccaatgt atgattcctc
     2461 tttgaataac caatattctt ataaaaaaaa taactgtaat cggaaagaca ttttttggta
     2521 atgcaaatga aatgttaggg aaataaaatc agtgtttttc gaaatccttt taaacaagaa
     2581 cgtagacgta ggaagaactt gaaaatgaac agaagttgtt cattacggta aattacaata
     2641 tgattacaaa aaacaacaaa gctcttaaat aacttttaat aaatattaag agaagttata
     2701 attattaata atagataaat tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa
     2761 gaacggagac gtttagaaca gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc
     2821 gtgggaactg cgatggtagg ctgttctgtc gctgccccat tagtacagcc ggttcctgtt
     2881 caagcggatg aaacacctgt tcagttcggc gcaagaatca atacagcggc atttatcaca
     2941 gaaattgcta cttatgctca accaatcgct caagccaacg atctatatgc atcagtgatg
     3001 atcgcacaag cagttgtaga aagcggttgg ggcagtagtg cgttatctca agcaccttat
     3061 tataatcttt ttgggataaa aggcagctat caaggacaaa ccgtctacat ggatacgctt
     3121 gaatttttaa ataatcaatg ggtaaataaa aaggagcctt tccgacaata tccttctttt
     3181 gctgaatctt tcaatgacaa tgcctatgta cttagaaata cttcatttgg caacggttat
     3241 tactatgccg gtacttggaa gagcaacacg aaatcctata cagatgcgac agcttgtttg
     3301 acagggcgtt atgcgactga tcctggttac gcaggcaaat taaataatat catcgcaact
     3361 tacggattga cccaatatga tacaccagct agcggaaacg caggcggtgg agcgactaca
     3421 ggtggcaata atactggaag tgcaggtagt actggcggtt caagcaattc tggtaataca
     3481 aactcaggtt cagcaacttc gaccacttat actgtccaat ctggtgattc tgtttgggga
     3541 atctcaaata aattcggtat cacaatggat caattgatcc aatggaacaa catccaaaac
     3601 aatttcattt atccaggaca aaaactgaca atcaaaggta gccaggaaaa tggctcttcg
     3661 acgaataatt ccggtaacaa taccaattct tcaggaaatg ctggtacatc aaacggagga
     3721 caaacaactg gtgcgaaata cactgttcaa tctggtgatt ctgtttggaa gatctcaaat
     3781 gatcacggaa ttacgatggc tcaattgatt gaatggaaca atatcaagaa caatttcgtt
     3841 tatccaggac aacagctaat cgtatcaaat ggcggttctt ctaatgctgg agcaaatact
     3901 ggctcaaata ctgggaatac aagtggctct agttcgtcca gctcaagttc ttctaataca
     3961 gcttcaggaa cgaaatatac agttaaagcc ggagactctg tttggagcgt ctcaaataaa
     4021 tacggaatta cgatggctca gctaatccaa tggaacaata tccaaaataa ctttatctac
     4081 ccaggacaac aactgactgt ttcaaacagt ggatcaacaa acagcacgtc aggtaataca
     4141 ggttcgtcaa ccaatacaaa taacagcagt tcagttaata ctggcagcag ttcgtctcaa
     4201 ggcacttata ccgtgaaagc aggtgaatct gtttggagcg tggcaaataa aaacggcatc
     4261 acgatggatc agttaatcga atggaataat atcaaaaaca acttcattta tccaggacag
     4321 caattgatcg ttaaaggtgg aacttcggca aatacaaata caggttcaac tacttctgca
     4381 gcgaaaccaa atacaccgaa tacgtctgct acgacaagta catcatccac tggtaatacg
     4441 atgtataccg tgaaagcggg cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg
     4501 atggatcagt taatcgaatg gaataatatc aaaaataatt tcatctatcc aggacagcaa
     4561 gtgattgtga agaaaggttc atctcaatca acaaaccaac aatcagttac tggttcgaaa
     4621 acctatactg tgaaatctgg agaatctgtt tggagtgttg ctgacagtca tggaattaca
     4681 atggctcaac taatcgaatg gaacaatatt aagaacaact ttatttatcc aggtcaaacg
     4741 ttgattgtag ctaaataaaa tagctgttcg aagaaataac gattgaattc cacaaaaata
     4801 tttgttacta taaggctact taaatatgac aacacttgaa aaggaagtag taaaaagact
     4861 gaagtttcag agagcgcatg gctggtgaga atgtgcacgg atgcttttga actcgccttt
     4921 gagttgccct tgctgaatac ccgagtaagc aagcggcggt gacggccgtt acaacgttcg
     4981 aggccttttc actaaggtaa aattaggtgg taccgcgtag attacgtcct ataaaaagag
     5041 caatcttttt ataggatttt tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg
     5101 aaataaaaat aaacagattc tgccggtttt ttcgttatat ttataataga aacaagtgtt
     5161 ttcaatagaa caggaggaca gcaaatgagt tacaatcata aagaaattga gaaaaaatgg
     5221 caaaaatact gggcaaaaac gaatactttc aacacccatg atgatcctga aaagccaaaa
     5281 ttttacgcac tggatatgtt tccctatcca tccggtcaag ggctacatgt agggcatcct
     5341 gaaggttata cggcaacgga tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg
     5401 ctgcatccaa tggggtggga tgcattcggt ctgcctgctg aacaatatgc gctagataca
     5461 ggaaacgatc cagctgagtt tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat
     5521 tctttaggat ttagttatga ctggaatcgc gaagtgaata caactgatcc ggagtattat
     5581 aaatggacac aatggatctt tacgaagctt tatgaaaaag gtctagctta tgaagcagaa
     5641 gtagcggtaa actgggtacc tgaattagga acagttattt caaatgaaga agtgatcgat
     5701 gggaaaagtg aacgtggtgg ctatgatgta gtcagaaaac caatgcgcca atggatgctg
     5761 aaaatcactg cttatgctga tcgcttattg gatgatctag aattagttga ttggccagaa
     5821 agcattaaag aaatgcaaag aaattggatc ggtcgttcag taggagcaaa tattgaattt
     5881 aaagtggctg gtacggataa atcctatacc gtatttacga cacgaccaga tactctattc
     5941 ggtgcaactt actcggttct tgcacctgaa ctagatcttg ttagagaaat tacgacccct
     6001 gaacaaaaag ctgctgtaga agcttatatc gaagagacag ctaaaaaatc agatctaaag
     6061 agaacggatc ttgcaaaaga aaaaacagga gtatttactg gtgcttatgc aatcaatcca
     6121 gttaatggca aggaaatccc aatctggatc gccgattatg tattatcttc atacggcaca
     6181 ggagccatca tggctgttcc agcacatgat gaacgtgact ttgaattcgc tcaaactttc
     6241 ggactagaaa ttcttccagt tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa
     6301 gatggtacgc acatcaactc tgaatttcta gacggcatga acaaacaaga agcaatcgat
     6361 aaaatgaatg aatggctaga agaaaatggc gtagggaaaa aagaagtcag ctatcgctta
     6421 cgtgactggc tattctcccg tcaacgttac tggggtgaac caatcccaat catccattgg
     6481 gaggacggaa cagtcacagc tgtcccagaa gaagagctac cattacggtt gcctaagaca
     6541 aaaaacatca agccaagcgg tactggagaa tctccattag caaatatcga ggagtgggtc
     6601 aatgtcgttg atcctgtaac aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa
     6661 tgggcaggaa gctcatggta ttacctgcgt tatatcgacc ctcataacaa aaaagaacta
     6721 gcagattatg aaaagttgaa gcgttggcta ccagttgaca tctacattgg tggagcagaa
     6781 catgcggtac ttcatttact atatgcacga ttctggcata aattcttata cgatatcggt
     6841 gttgttccta caaaagaacc attccaaaaa ctatacaatc aaggaatgat cttaggcgaa
     6901 aacaacgaaa aaatgtcaaa atctcgtggt aatgtagtca atcctgatga tgtggtcgaa
     6961 acttacggtg cagatacact gcgcatgtac gaaatgttca tggggccatt agatgcttcg
     7021 attgcttgga gcgaaaatgg cttagaagga agccgtaaat tcttggatcg tgtatggcgt
     7081 ttgatcgttg atgagaacaa taaaatgcgt gaccgtatca ctacattgaa tgatggcaag
     7141 cttgataaag tgtatcatca aacagtgaaa aaagtgacag aagactatga aaatcttcat
     7201 ttcaatacag caatctccca attgatggtc tttatcaatg aagcctataa agtggacgct
     7261 ctgccttatg aatatatcga aggatttgtt caactactcg caccgattgc tcctcacatg
     7321 ggtgaagaac tatgggcaat ccttggaaat gatggtggta tttcttacgc accatggcca
     7381 acatacgatg aagcagcact tgttgaagac gaagtagaag ttgttttcca agtaaacggt
     7441 aaagttcgtg cgaaaagcaa tgttccccga gacttaggaa aagatgagct agaaaaagca
     7501 gcattagcca atgaaatagt gcaagaatat attgaaggca aaacagtcag aaaagttatt
     7561 gcggtaccaa acaagttagt caatatcgtt gctaattaat tggctctttg tcaataagga
     7621 ctgatgagtt gtgcaaaatc aaatctgggc cagaatgaac ttcattctgt ctcagatttt
     7681 ttgttatttc aaattgatta attgattgat caaaaaggtt ttgattttca tgttctcaaa
     7741 cacgttcaat gtctttttct tctgaatggt cttgtaagag atctctgtaa tcatcaaatt
     7801 tttgtctatt aatcttagta ttttttgatg tataaactac ctgaaattga gagtacatac
     7861 caaattcata aattttttat tagtgggact agtgattgta tggattttga cgcataatga
     7921 atgaagtatg agaaaaggag agacaaatgg atcaagaact atttattatc gaagtattat
     7981 cagctaaaca aaaaattatc gagaaagcga aaaaaagagc gaaaagaata atgttcaagc
     8041 ttttaagctt agtatttttc tctggtttgc tttttttcgg agctgtatcg ttcctatatc
     8101 gccatatgag tgcagtagtc cgttcagctg aagggatgca aaaattaaat aatttgtatc
     8161 acttttcgtg gggagttggc ggaataacaa ttgttgtgct tattgttttt cttctactta
     8221 tttggctccg ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt caaagagcac
     8281 aacagttttt acataataaa ccaacttctc aagatgatga ctatctttat tttgagaaga
     8341 cttcgacgag tcaagctttt cggatacaaa aaagtgattg ccagttattg ctgagatcac
     8401 ttgatgattt acctatctac ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc
     8461 atattttttt actagaaaac gaacccagat gggaaaaaag ccagtcttcg cctggtggat
     8521 acctgacaac taagatgagt acattagtca ctggtttttt cctcattctt ttagctggaa
     8581 caatctatta tgggagatac actcagccaa aaatgtcttc tcatataaat gctgcttctc
     8641 acatgacaat gaactcaact aacgatacgc atatacaaaa gacaaatgaa gggttgttag
     8701 ctgatcctaa gcaaaaggta aatttgacaa cacaaaagat aaatgatctc aaacttgata
     8761 cagagacgca ctctctctat atgacaacag atagtggtgc taattgggta tttgtgccga
     8821 ttgattcgca atggttgcgt tttggcgaat acaccctgac aacggggctg attcctgaag
     8881 gttattggat ggacaacaca tatgatattt ccgccgattt ctcatggttt atctattctt
     8941 ctggggatga tgtacgtctg ctgacttcaa gagataatgg taaaacatgg caaacgtcta
     9001 ttatcaccca atcagttcag ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg
     9061 gtctatttat ctgtacatca gccagtggga tgtctgcaga aactttgtac atttacacta
     9121 ctatggatgg cggaatcact tggataaaaa gtggaagtac aatgatcaat cagccagtac
     9181 aaaatgtcag cttcgttaca acttctttag gttttgtttc gacacgtgaa agtttgtttt
     9241 atacaaacaa cggaggacgc tcattcaaag aatcagttct gtcgattccg gaaaactatc
     9301 agacaggggg gatagatctt tttcaatcac caaatgaagt tactcaaata agcgcgaata
     9361 cgttagagac aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg
     9421 cctgcctctt ccgttcaact gataacgggg aaacatggca attcgttcag caactatcgc
     9481 aagtagaaca aggagattaa agaagagaga ttgtgagcct gacgttcagc accgagtcac
     9541 aagaaacaaa ttcaaaaaac agcttctcat atttgttgaa tagtgtaact cgtgattcgg
     9601 agttgacaag agcaacaaga agcgattttg aagtatagct tctcgtattt tacaagatcg
     9661 tgtcttattg ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac
     9721 aagtcttttg tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag
     9781 tattattctt tgtgtttcta gtggcaccac tagtacattt aaaaaagcta tcgatcatgg
     9841 actaatcata catgatcgat agctttttag ataatgtttg ttacgctatc tgaaattgac
     9901 tattataaag actggcataa agcgttggtg attggagtag ttcagaatgt gttccttttt
     9961 cgacgatatc gccattcttc attacaagga tcatgtctgc attttcaatc gttgacaaac
    10021 ggtgtgcaat cacaaaactt gtcctatctt ttgtcacagc ttccattgct tgctgaatca
    10081 atgcttctgt tcgtgtatct acgctagaag ttgcttcatc taaaataacg acaggaggat
    10141 tcgctagaat gactcgagca attgtcaata attgttgctg tccttgagat aacgtgccgt
    10201 tttcgctaga aatgatcgta tcatatccgt ctggcagtgt tcgaataaag tgatcacatt
    10261 gtgcgatttc agccgcttcg ataatttgtt ctcttgtggc atccattttc ccgtaggcga
    10321 tattatcagc taccgttcct tcaaacagcc aggtattttg taaaaccatc ccaaaagaac
    10381 gacgtagttc tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg atctttcctt
    10441 tattcagttc ataaaaacgc atcagcagat tgactaaagt cgtttttcct gcaccagttg
    10501 gaccaacgat cgctaccgtt tgttttggct ttactgaaaa atcgacttga ttcatcaata
    10561 ttttatctga tgtgtaacca aactgtacat ttttgaaagc tatttttcct ttcggttgtt
    10621 ctaaaagtct tgcatctgga aggtctggaa tttcatctgt ttcatctaat atctcaaata
    10681 tcctatcgat tgctgccatt gcggattgga tcgagttgat cacataagaa gcggtagaaa
    10741 ttggttcaga aatctgattg atgtactgaa gataagcttg taagaagccg atcgtcacac
    10801 cacctgatag aacaagcata gcaccgaata ccgcacttat gatgaaggcg agttgattaa
    10861 tgaaacgaat ggccggatag atagcacagt tcataaattg agcacgttta aaagcgttgt
    10921 attgcttttt atttacttcg tgtatagcag cttctgcatg agcttgctga ttgaatgttt
    10981 tgaccactag attaccagct aagatttctt ccattcgatt atttaaaaca cccagttcat
    11041 actgattctg ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta acaattgaac
    11101 tgccaacgat cagcaataag acgagcaaag taagcttgct ttcgatataa aacagcatca
    11161 gaccagcagc agctattgtt acgacagaag taaaaaattg attgatacct gtcaataaca
    11221 cttgagaaag ttgattgaga ccggtagttg tccgactaat gatgtcacct acttgatgat
    11281 tgtcaaaaaa cgacatcggt aatgttttga atttttttgt gatttctttt cgcaccctca
    11341 atgtgatctt ttcgcttaac gctgccattg tccgttcctg aataaatgaa gtgatgctgc
    11401 ttaagactga acagccgatc agcaataaga cagggaacaa caatgcttgt ttgatatcac
    11461 tgaaagaaag ctgtccaata cctgtttttc ttatttctga taagagatca tcgatcccga
    11521 ttcccatgat caagggcatg gctacgacta ataggttccc gaataaactg catagaaaca
    11581 atccgtaaaa atatttatgt tcaggtttga tcattttcca aaaacgtatc aaagcacgcg
    11641 tgttcaacgt ttttttcttt tccatcgtat cccttctttc tatattcctt gtgattttgc
    11701 aaactcctga taagtttggc aattagacag tagttcttca tgagtacctt gtccaacgat
    11761 ttttccttca tctaaaacaa tgatgttatc tgcttgttgg attgtactga ggcgttgagc
    11821 aacaatcacg agagttttct ccgacatgtg tttatgaagg gctgctctta atgctgcatc
    11881 tgttttatag tctaaagcgg agaaactatc atcgaataca tagatctctg ctggtttgat
    11941 caacgctcga gcaatacata tccgctgttt ctggccccct gaataattag aaccgccttg
    12001 agcaacaaag ctgtccaatc cgtcaggaag ggtgtccact agttcttcta attgagcgat
    12061 cttgatcgct ttagacaact cttctgttga ggcatattca tttcccatct gcaagttgct
    12121 taaaattgtc ccgctgaata agaaagcctt ctgagggaca taactgatgt gggagcggat
    12181 ctgttcttga gtcatttgag taagaggaat accaccaagg gttacttttc ctatcgtagg
    12241 gtcactaagt ctcaagagaa gtttcgccaa actacttttt ccagatccgg tcccgccaac
    12301 gatcgctgtt gtttttcctt taggtattgt gaaatcaata tcttctagaa ctggatcaga
    12361 gatttcagta tatttaaatg tgacatgttc aaaagcagct gcatttgtct gatcaaaggt
    12421 tggttcagct actgttttga tcgtctcttc ctcaatttcg atctctgctt gcagtatttc
    12481 ttctatccgt cgtaatgagg caaacgattt agggataatg accaatacca tagctgcgat
    12541 catcaagtaa gcaagtgtca gcatagagta ttcgactaca gcagtcacga caccgatttg
    12601 taatgctcct tctgctacaa gatttccgcc aaaccacaaa atagaagcat aaacgattcc
    12661 catgagcaaa aaagcaattg gagtgaccca agcaaatatt cggttcactt tgatcatttg
    12721 attagcatag tcagcaaagc tcttattcgt tcgcttttct tcatatgatt gattatcaaa
    12781 ggcacggatc atattaatcc cagtaaagaa ttctctcaat gtgatggtga tccgatccat
    12841 ttttggctgg atactcaaag ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca
    12901 tacataaaaa acaacagaga tcaatgtaac ccatgttagt aatagagaat aacgaaacat
    12961 catgagaatg gtgaaaacgc aaataaccgg tgctggtaag atcatttggc aaaataaaac
    13021 aatcatttgc tggacattat cgacatcatt cgtcattctt gtcaaaagag atccagaact
    13081 aaaacgactg atatccttca ttgacagctg ttggaattta cggaaatatt tctcccgcgt
    13141 ttggaatcca aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat
    13201 tgttcctaga aacgctacaa gcagcatttc aactccaatt ttttttataa ctgaggcttc
    13261 gccacttgct atacctgtgt cgatcagctt tgctactaat tttggtacac ctaatgtacc
    13321 ggcgatttct aaacagatca tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa
    13381 gaatgttttt aagatagaca tattattcct ccttttttcc tgatactttt cgattctata
    13441 ccttcaagta acttgaaggt caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa
    13501 atgaaaaaaa tgtatacgat tggccaagta tcggaactat ttgatttgcc aaaatcaacg
    13561 atccgctatt gggatgaaca aggattaatc cgttcaagta gacaagaaga gaatgattat
    13621 cgattgtttg atatcgatga tatttttatg ctttatgata tcgtttttta tagaaaatta
    13681 gatataccga tcaaacagat gaagaattta tatggaaaaa cattgacgga gttatatgag
    13741 acacttgatg aaacagaaag aagaatccat aaagaactgg tggtgatgaa acaaaaacaa
    13801 aaagagataa gggagcaaaa aaaacagttg aaattgatga tcgatacaaa cgaggaagaa
    13861 tttcctgtag aagagatccc ttttgactgt atgatctcaa cggagtttga agatattgtg
    13921 gaaatcaaaa aatttctacc taactattca agctttggga tgatgagtat gccagcttcc
    13981 agttcacaga cgatgtatgg attttttatc gaccctagtg aagtacattt atttactcaa
    14041 gacgtgattt gggagaaaaa agatacggct gtttatcgtc gattcttatt gaaatccgag
    14101 atgaaccatg cggaacgtaa taatatttta gaaatcagag aaagaatgta tgaaaaagga
    14161 tggaaaacag gagaagtgat cggccagtat ctattgacga atacggatga aaacaatatc
    14221 agaactgaat attatcatgc atggatagaa atgaaaaaat aggaaatatg tgtttaagat
    14281 aactcgaaaa tgatggttgt tttagttata aagaaaaatt ttctggttcg attacgggtt
    14341 taatgtgggg gattattagt cgtggataga tagaatttta gttttttaga ggaaatatgt
    14401 ctttaaaaat ggaatggttg caattcttag cgatatatac gatgtaattg tcctcttaaa
    14461 aaagtatgga aaaataattg tattgggtgt tatgtataat tcatggtgaa ctgagactaa
    14521 tgagaagaaa aattcatatt gttatgagtg agattgtatc agatgagaat gataaattaa
    14581 ataggtgcat aaaagcatta cac
//
LOCUS       DACCXM010000066        14194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCXM010000066 DACCXM010000000
VERSION     DACCXM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.068"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(51..1598)
                     /locus_tag="IVY61_001746"
     CDS             complement(51..1598)
                     /locus_tag="IVY61_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7970860.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IVY61_001747"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IVY61_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP7970861.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IVY61_001748"
     CDS             complement(2043..2237)
                     /locus_tag="IVY61_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970862.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(2463..3665)
                     /locus_tag="IVY61_001749"
     CDS             complement(2463..3665)
                     /locus_tag="IVY61_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7970863.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(3668..3862)
                     /locus_tag="IVY61_001750"
     CDS             complement(3668..3862)
                     /locus_tag="IVY61_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7970864.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(3909..4757)
                     /locus_tag="IVY61_001751"
     CDS             complement(3909..4757)
                     /locus_tag="IVY61_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP7970865.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(4962..5312)
                     /locus_tag="IVY61_001752"
     CDS             complement(4962..5312)
                     /locus_tag="IVY61_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970866.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            5907..6353
                     /locus_tag="IVY61_001753"
     CDS             5907..6353
                     /locus_tag="IVY61_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7970867.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            6977..7969
                     /locus_tag="IVY61_001754"
     CDS             6977..7969
                     /locus_tag="IVY61_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7970868.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            7985..8869
                     /locus_tag="IVY61_001755"
     CDS             7985..8869
                     /locus_tag="IVY61_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7970869.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            8918..10525
                     /locus_tag="IVY61_001756"
     CDS             8918..10525
                     /locus_tag="IVY61_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7970870.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            10526..12010
                     /locus_tag="IVY61_001757"
     CDS             10526..12010
                     /locus_tag="IVY61_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7970871.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            12187..13179
                     /locus_tag="IVY61_001758"
     CDS             12187..13179
                     /locus_tag="IVY61_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7970872.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            13231..14100
                     /locus_tag="IVY61_001759"
     CDS             13231..14100
                     /locus_tag="IVY61_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7970873.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         4335 a         2423 c         2877 g         4559 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttacg ttgaaaaacg ttgatttatc
     2341 ggcattcccc atccatatta tatttacgtc ttattcccac tcaatatgag tagcctcgta
     2401 aagaagctat tatatcagtg ctttttcagc ctattcctca ttttggtagg gaaatggtag
     2461 ggttagattt ttactgcttg atttccatta aagtttgcaa ctttagattt tgttgcacag
     2521 gtaaatttaa tgtttcctgt catttgttct gcaattcctt catcattacg gctccataga
     2581 tgagcgtagt gctttaaagt gatttcaggg ctagaatggc ctaaccgttg cgacacgacc
     2641 agaatgtctg cgttaaactc attgattagg tagctaacat gagaatgacg taatcctttt
     2701 ccttgtatta ggggtacctt agctagtttg gcataacgct gaataattcg ttgaacagtt
     2761 gaacgataga gtgggaggtc agtgtagcta agaataaatt gttctacacc atgatttttt
     2821 tgaataactt tccacttttt tagcacttca attgtatcat cgtccagaga aatgatccgc
     2881 aagccgcttt cagtttttgt gtaaggtttc cgaataaaat ctttttgatt tgtcatttcc
     2941 aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca cgtcattcca attaagtgct
     3001 aatccttcgc ttacacgaac accagtcatg taatatagcc agatcattaa aaaacacata
     3061 tgttcgtaga aatcatcttt ataaattttt gccaaaactt cttcaaattc ttcttttgtc
     3121 cagtaaggaa cgattgattt tccttttgga attgcttttg ttcttttgga aatattctca
     3181 ttcagaaatt gcagggtgac cgcatagtct aatgatttac gaaacatacc gtacataagt
     3241 gaactgtatg cttgtgagta tccgctatcg ttcaataacc aaattcgata attttcacaa
     3301 tctcgcacat tgatttcacg aagcttctta tcaccgaaaa attcagtaat ctgaatcaat
     3361 ccacactcac gagaactcca agtacttttt tgtacgctag ctttgtagtg tggaacgtaa
     3421 gactgctcca taaattcttt gtaagttaaa tcataattcg caaatccatt tacaaccatg
     3481 tattcatttt ttatacgcat gacttctttg tgtgcttcac gagctgatga gaatcgtttt
     3541 cctagagaat cttttgttcc tttcttctgg atcctctttc cagtcacttg atctgttcct
     3601 aactcaacta gataagaaaa gcgaccattt ttatcttgat acacacaagg gtattttgtt
     3661 ttcatagtta ttcctcctgt gaaagaggaa cacctataat ttcttcaatt acttgtcggg
     3721 gtactacccc aactctttta ttattataat atggatatcc ctgatttacc attcttgttt
     3781 ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa tcctagagcc tctaaatctt
     3841 ttcgacaaat tgtatttttc attgtataaa ctcactttca aagaaaacac tatcattggg
     3901 taatagtgtt atttctttat ttttgtataa tgaacggtac tttcttgaaa atccatcact
     3961 tgttcaggac ttcccaactc atttaaagct aaagcattgt aaaaaaatgg acgtctaact
     4021 atatattcgc ttgtatatag tatatcttga ttcgtggaat catagggttt ctttaccatc
     4081 tttttggtga cagttggtag tttgagattc cgggacttga aaaaagcttt tttcttatgc
     4141 tctttcaatt ccagctcttt atcgaaatat ttgctgatat agcgcccgcg attttcgata
     4201 ctgtctacat cgatcttgtt gatcttaacg aaaccctgac cccaaatcgt agttaaccgg
     4261 ttaaatgaga taaaggggaa atcaaacaag ataatatggt agtggattgc accgcgttgc
     4321 tgcttttccc acgtggcgag atactttaaa tttgcttttt tagttttata gaggtcataa
     4381 ttcagtcgtt tgataaattt tttaaattca ttatttgcat actcaatgtc ttgaatgttt
     4441 tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa aattcgtgtc tacaaggcgg
     4501 gcaatctcaa atcgcatact ttgataatgt ttctgtttgc gttttaaact gtcatattgt
     4561 ccttgagcgg ataattcatc atagtttttt cttttttcaa gagtaatatc attttcataa
     4621 atccaagcta atttttgttt tcgttccaga tttacttcgt tttcgatcgt tttacgtttg
     4681 ggagtaatga ttgcttcata atcataaatt tcaatatacg tgggagtttc aatgattttc
     4741 tgattataag cttgcaaaaa tcatcactcc ttgttgcgta ttcgattgtc aaagaaccag
     4801 tcattttctc ctgatgttga tctataaatc aagttaaggg ggaaggcctg cgtagcagcc
     4861 cttctagcct atggggtgtt gggcggtctt aacggacaga aacggctctg tgggcgctgc
     4921 cccgccgttt cgtcctaacc acccaacact ccatagtata gttagttttg tgtagtggta
     4981 ccttgttttc tgggaatcaa agttgccgta tcagcggttg ccgataaccc ataactgaca
     5041 aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat tggtaaatgt aattatggct
     5101 aaaccttgtt gggtaagttc ttccggcgtg atcacaggat gttcagattt gactttgaca
     5161 ctgatttttt cgtagcgaag ttttggtagg atacattcgt agctccaacc tagaatttcg
     5221 ccctcctttt gccagcgagt gacattgacg acttggtatt ctttttcgag tgtttctttt
     5281 ggtaaactga gatcggtaat ttttattggc atcacaattc tcctttttta tacgaaagtt
     5341 tatacgattt gattaaaaaa tatagatggc catctacgac ttgtattata tacgagaact
     5401 tatacgtagt caagagattt tctaaaaaaa tatatacgat tttttatacg agtgttataa
     5461 tgaaaaaaat gataaggagg aatttgaata tgtttggttt agtacaacct aataaagtga
     5521 aggtgggcca acgaatcaaa gaaataaaag acggaatgtc tctctctttt acagaattag
     5581 ggaatcgatt aggtttaaaa aagccgacga tcagttccta cgttcaaggc tatgcgttgg
     5641 ctcctaagag tgtgatcaac caattatcta gtatcagcgg aaaacctgtc ggctggtttt
     5701 attttgggca tgtggaagaa tatatccgag agtatttgtt tctcattggt cagaaaaaaa
     5761 tagtggacga ttatcctgaa atcattgaga aaataaagca agaattttat acgggcgagt
     5821 tcaaaaatcc tgcttgggag aatgagctag gctatccttt ggaggaattt atccaagaat
     5881 gttttgtaga atattcgagt gaaatcatga aggactatgt cgtacagatt acgaaagagg
     5941 tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa agcaaaagaa gaagctgtca
     6001 cgattctttc atctgggatc attgactata tcgagatgag tggtgagatc aagtatggag
     6061 agaaagatcg cattgcgaaa atggtgaaag aatcaataaa aacctttgat gtgaaacaag
     6121 acctgacatt taatgattcc tatttgattg gaaagttgat caatattctt ggtacagatg
     6181 aagatacgga gctgtttctt tcttctttat caatgatatt tactgataag catttttcca
     6241 ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc gatgcgacca gcgttaatca
     6301 aactctatcg ggagaaaaca ccagatgaat tttatgattg gtttgagaag taaaataaga
     6361 aaagtggctt agattacaag ttggtgaaaa aatttttttc agcataacat cagtaatcta
     6421 taaaggggtt gttgattata ctgtagaagt taagtactca agagattttt tgtacgtaaa
     6481 caaagcaaaa aagggtgcag tttcactaga aaatgtactc ttttttgtga tttttcttag
     6541 ttatagagtt tttttgaaac tttaaaaatg agaaaacata ttgattttaa agagtgtaga
     6601 catagcttct tttgctaaat agattaaact tactggtgag tggtcttgaa catactttta
     6661 aatcacattt tactgagttt tattggattt tataattcat tggaaacctc gtacttaatc
     6721 tgtttttatc tatcgactag aatctgtata tattccagct atcaatgagg ccgcagaaaa
     6781 gggtgcttaa aaataatctg ttgtttaaaa tattattatc agaatactta gatacaaaat
     6841 tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg atatggtaat ctataaacga
     6901 aaatagtggt aacgtttcca aaatgcatct tcgttaaatt tttatttttt taaacgaaaa
     6961 tggaaacgtt tctaaaatgt attgggagga gattgaattg gaaaagacat tgggaccagg
     7021 tggtgagata agggcggtac aacgggataa aaaaaatatt tcgaagtggg aaaaattaaa
     7081 gaaacagttg cggcgtgata agtggatgta cttgttgctc ttaccaggta tcatcatgac
     7141 gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt gcatttaaag actataatcc
     7201 tatgctagga attttggaaa gtccttgggt aggactagat cactttaggg aatttatcag
     7261 ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa attagtattt atggtttatt
     7321 gttaggtttt tttccaccaa ttattttagc actgagcttg aatcaaatta tcaataaaaa
     7381 aataaaacag aaaatccagt tgattctata tgcgccgaat tttatctcag tagttattat
     7441 tgtggggatg ctgtttttgt tcctatccgc aaatggacct attaatgggt tagtagaaaa
     7501 ggttacgggg caaccaatta tgttcatgtc taatccagct tatttcagaa cgatttatat
     7561 tttatcggga atttggcaag gaatcggctg gtcatccatt ctttacaccg caacgctggc
     7621 gggagtcagt accgatttat atgaggcagc aaatatcgat ggggcaaata ttttacaaaa
     7681 gatttggcat atcgatttgc ctgcattgag gcctgtgatg gtgatcagct tcatcttggc
     7741 cgccggcggt attatgaatg taggatatga aaaagcctac ttgatgcaaa cttcaatgaa
     7801 catccctgcc tcagaaatca tttccactta tgtatacaaa gtcggcttgc aatcaggtga
     7861 ttatgcctac tctgctgctg ttggtctgtt caatatgatt attaatgtcg ttttattact
     7921 gattgtcaac aatataacca agcggctaaa cgatggtcaa ggcttgtaat gaaaggagaa
     7981 aattatgaat agtgctttat caaaaatcga ccgtcaaatc ttatggttga atcgagtgat
     8041 tgtaactttt ttagtcgtca tcaccattgt tccgctgatt tatgtactgg tagcttcttt
     8101 tatggaccca gctactttga tcaataaagg tataagcttc aatccgaaag attggacaat
     8161 acagggatat caacgggtat ttgcagatga ttccatttta cgcggttttg taaattcgtt
     8221 gttttactct tttagcttca gcttattgac tgtatttatt acgatgatca ctgcgtatcc
     8281 tttgtctcga cctaacttgg cggggaaaaa gattattatg actttttttg tcattacaat
     8341 gttcgtgggt ggtggcttga tacctaccta tttattggta aagaacttgg gcatgttaga
     8401 tagtgtatgg gcgattatcg taccaggtgc tataaatgtg tggaatatta tcttagcccg
     8461 tacctatttt agtgctttgc caaaagaact ttctgaagct gctactatcg atggcgccaa
     8521 tgacttgcaa atactgttca aagtcatgct tccattagcg aaaccaatta tgttcgtatt
     8581 gtttttatat gcctttgttg ggcaatggaa ctcatacttt gatgccatga tttacatcaa
     8641 agatcccgac ttacagccgt tgcaactcgt attaagaaat attttgatcc aaaaccaacc
     8701 ttcacaagac atggtgggag ccaatactgc catggcagaa atgaaacaaa ttgctgagtt
     8761 gatcaaatac gcaacgatcg tcatttctag tctgccatta atcataatgt atcctttctt
     8821 ccaaaaatat tttgataaag gtgttttagt tggttctgtt aaagggtaag tattgttcac
     8881 tatttgtatg aataaaaatt ataaaggaga ttttaagatg aaagcaaaat ggatgctgcc
     8941 aatcgttttt acttccgtac taattttggg ggcttgtgga ggctcaaaag aaaagagtaa
     9001 atcaagccct gattatgaac tagaaaatgt ctcttttcca ttgaaagaaa aagtatcttt
     9061 aaaaatgatg tctcaaagtg cccctttggc tcccaatgat cctaatgaca aattgatttt
     9121 ccaacgatta gaagaagaga ccaatctcca tattgattgg acaaactaca gtagtgattt
     9181 tgccgaaaaa agaaatttgg atatcgctag tggtgattta ccagatgcca tcttcaacgc
     9241 cgctgcaggt gactatgatt tattaaactg ggcggaaagt ggcgttattg taccgattga
     9301 agatttgatc gaagattata tgccaaatct gaagaaaatt tttgatgaaa accccgaata
     9361 ccgacaaatg tctactgcac ctgatggtca catttactct atgccttgga ttgaagaatt
     9421 aggcgaagga aaagagtcga ttcatacagt aaatgggatg gcttggatca acaaggagtg
     9481 gttggataat ttgggtcttg aaatgccgca aaccactgat cagttgatga ctgtgttgga
     9541 ggctttcaag acgcaagatc caaatggtaa tggtgaagca gatgaaattc cttttagctt
     9601 tattaacaat ggaggaaacg aagacttaaa atttttaatg ggggcattcg gtctaggtga
     9661 taatgatgac cacttagtcg ttgataacga tggtcaggta caatttactg ctaacaaaga
     9721 agggtataaa gaagccatca aatatttcaa tgaaatgaat aataaaggtc ttattgataa
     9781 agaggctttc gaacaagatt ggaatacgta tgttgccaaa gggaaagaac ataagtatgg
     9841 cgtgtacttt acttgggata aagcaaatat cacgggtgca gatgatagtt atgacgtctt
     9901 gccagtctta gcgggaccag atggaacgaa acatgttact cgtacgaatg gcatgggttt
     9961 ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac ttggaactta cagctaggtg
    10021 gatagataaa atgtatgaac cgttacagtc tgtccaaaat aactggggaa cctacggtga
    10081 tgaagcacag gcgaatattt ttgaattaaa ggatggtatg ttaaaacatt tgcctttaga
    10141 ggggacagca ccgggggaac taagacaaaa aaccgaagct ggaggaccat tagcagttct
    10201 tgattcttat tacggcacag tcactactat gccagatgat gcgaagtggc gtttagattt
    10261 gatgcatgac acgtatttgc catatattac agaagacaat atctatccac gtgtcttttt
    10321 agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg gatatgtggg attacatcta
    10381 tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat gaagaatggg aagaatacct
    10441 tgctgaattg gaacgtgtag gtttggaaga atggattcaa ataaaacaaa ctggctatga
    10501 taattttttg aagggagcac aataagtgtt agtagaagaa acggcgattt tggataaagc
    10561 aaatcgattt attgaaatga tgcgaggaaa agttaaccct cagtatcgac aaaactatca
    10621 tttttccgca cccatcgggt ggataaatga ccccaatggg tttatatatt acaaagggca
    10681 ttaccatctc ttctatcaat actatcctta cgatagtcga tgggggccca tgcattgggg
    10741 gcatgcgcgc agtagtgatt tggtccattg ggaagatcta ccagtagcgt tagccccaga
    10801 tcaaccttat gacagcggcg ggtgtttctc aggaagtgca attgaaagag acggaaaact
    10861 atatttattt tatacaggct gtcaagaaat tgatggtatt agccatcagc atcaatgttt
    10921 ggcggtctct gaagatggta ttcattttag caaatacagt ggtaatcctg tggtagcaga
    10981 aaaacagcta ggtgaaaatg ggctaataca agaatttcgt gatcctaaag taattgttca
    11041 caacgatggt tactatatgg tagtagctac taaatctaca cagtctactg gtagaatagt
    11101 gatgtttcaa tcgtcagatt tgtatacttg gcacttttac tctgtgctgc tagaagggac
    11161 ttcagagcaa ggaataatgt gggaatgtcc tgatttattc catttagacg gacgagatgt
    11221 cttaatcatg tctccgatac aaatcaagaa gaacggactg gaatatcata atatcagttc
    11281 taccatggct tgtattggtg aggtaaactg ggaaacaggt cagatgatag ttgataattt
    11341 ccatgaaatg gattttggaa tggattttta tgcaccacaa acaacaagag atcctaatgg
    11401 tggacgggtg atgattgctt ggatgcagat gtgggatcgt acattgccta cacatgagtt
    11461 aggccatctt tgggcaggct ctatgagttt gccccgagag ttaagagtga aaaaaaatcg
    11521 attggtacaa aaacccatct caactgtata tagtcattta gtttatcaac aaggcagtga
    11581 aaatattgca gtgactgaca catcagtcgt atttcgaaac gcgctgtctg attcttcata
    11641 tttacatcta gtcgcagatt tggagaaagc tgaagttttt acaattgaat tactgcggtc
    11701 taagacggat tgtctcaaac tagcctatga cactgctaca caggtttttg aattggatcg
    11761 tcagaaacta ggaagagagc ttacaggggc ggaagaagac cctctgaatg tacgaaaagt
    11821 tgaagtacct ttgattgata atcagctcgt tcttgagatt tttagagata cctcatcaat
    11881 tgaagtcttt attaatgggt tagaagttat gacggctact ttctatgagc tggaaaaagc
    11941 tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt caagcttttg aaacagcgag
    12001 tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt atttaatagg ttttgaagga
    12061 taatgttttt agaaaagaac agatagctag atttcccatg gatttgctat tagtattttg
    12121 aaaaaattag taatttatga caaaattcat tatactaaac ttgtgcagaa atgtgaggga
    12181 tcaactatga aagcaacaat gaaagatgtc gcaaaattag caggtgttgg agtaggaact
    12241 gtttcccggg tattgaataa cggttctgtt aaagcttcta ctagagaaaa agttgaagca
    12301 gcaatgacag agttaaacta tcaaccagat ttttatgccc gggggttaaa gatgaagcat
    12361 acgaacacgg ttgctttgat tctacccacg gtatggcatc cattttttgg cgaatttgcc
    12421 taccatgtag aaacagcatt agaaagacaa aactacaagc tttatttgtg taactccagt
    12481 ggtaatagtg aaaaagaaca agaatatatc cagatggtca gccaaaataa agtagacggc
    12541 atcattggaa ttacttattc agatatcgat gaatacattt cgagtaatct gccttttgtt
    12601 agtattgacc gttatttcac tgaagaggtg atttatgtga ccgctgacaa tgcggccggt
    12661 ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc agaatgtagc atatatcggt
    12721 gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa aatattttga aatcgagtgc
    12781 cgtcaactgg ggatttccta taaagcgtta gatatgttag aacctttaga ccatctctca
    12841 gaacgtgtaa caaatttttt gttagagaat cccgatatcg atgggatttt tacgatcaac
    12901 gatccaatgg cattagctgt cattgatatg cttgaaaaaa tagggaaaaa cgtaccagag
    12961 gatgtccaag ttatcggatt tgatggtcaa aagatgacag aaggagatcc ttatctagtc
    13021 tcaacgattg cccaacctac aaagcaaatg gcggaagtgg cagtggcaaa attaattgct
    13081 gtcattaatg gagagacagt agaaaaacgt accgtattgc cgattgaatt tggcgaaggt
    13141 gacacaacga aacctttagt gagaaacgat tttgcttagt gcaaatacgt ttcttttttt
    13201 gaaaatgagt gaatgaaagg tgtagataaa atgttatatg gtggaataga agccggtgga
    13261 acaaagtttg tctgtgggat tggtaatgaa caattacaag tgatagaacg gaaaagtttt
    13321 cctaccacaa caccagaaga aacgatggag caagtgtttg atttctttga taagtacaaa
    13381 ggaaatatcg catcaattgg tgtaggagca tttgggccta ttgatattca aatcgaatca
    13441 agaacttacg gatatatcac caacacaccg aagttagctt ggcaaaatta tcattttgta
    13501 ggagctttgg aaaacgcctt gtcggttccc atctattgga ctaccgatgt aaatgctgct
    13561 tgttatggag aatatatggc agggtatgga aaaggtaaaa atagtgttct gtattacacc
    13621 gttggtacag gtgttggtgg tggtgctgta gtggatggga aaatagtcgc aggttttagt
    13681 catcctgaaa tgggacatat gctcttacaa cgtcataatg aagatcaatt tgaaggaatc
    13741 tgtccttttc atcatgattg tttggaaggg ttagcatcag gacctgcgat tgaaaaacgc
    13801 tttggaagaa aagcccagac tatctcattt gatcatcctt attggacgat tgaggcagat
    13861 tatcttgctc agtgtgtgta taacacaacg ttactatatg caccggaagt aataatttta
    13921 ggcgggggtg taatgaaaca gccgcaacta ctcgagttga ttcgccaatc gtttaaagaa
    13981 aaaatgggtt cctacgtgga tacgcctact ctcgaagaat atctactaac tccaaggctt
    14041 gctgacaatg cgggcttgat tggctgccta gctttagcaa taaagaaact caatcattag
    14101 aatagtattt attagaacta ttcataacat accggaagtt taaaagggta ttctgaaact
    14161 taaatagtct caattcattc ttctttcttg agaa
//
LOCUS       DACCXM010000067        14180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCXM010000067 DACCXM010000000
VERSION     DACCXM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.069"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="IVY61_001760"
     CDS             complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="IVY61_001760"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP7970874.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="IVY61_001761"
     CDS             complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="IVY61_001761"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7970875.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8128..9096
                     /locus_tag="IVY61_001762"
     CDS             8128..9096
                     /locus_tag="IVY61_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7970876.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9114..10019
                     /locus_tag="IVY61_001763"
     CDS             9114..10019
                     /locus_tag="IVY61_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7970877.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10016..10822
                     /locus_tag="IVY61_001764"
     CDS             10016..10822
                     /locus_tag="IVY61_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7970878.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10979..11947
                     /locus_tag="IVY61_001765"
     CDS             10979..11947
                     /locus_tag="IVY61_001765"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP7970879.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12264..12788)
                     /locus_tag="IVY61_001766"
     CDS             complement(12264..12788)
                     /locus_tag="IVY61_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP7970880.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13075..>14180
                     /gene="scm"
                     /locus_tag="IVY61_001767"
     CDS             13075..>14180
                     /gene="scm"
                     /locus_tag="IVY61_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7970881.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTS"
BASE COUNT         4018 a         3059 c         2799 g         4304 t
ORIGIN      
        1 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
       61 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
      121 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
      181 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
      241 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
      301 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
      361 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
      421 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
      481 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
      541 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
      601 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
      661 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
      721 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
      781 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
      841 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
      901 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
      961 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
     1021 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
     1081 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
     1141 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
     1201 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
     1261 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
     1321 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
     1381 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
     1441 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
     1501 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
     1561 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
     1621 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
     1681 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
     1741 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
     1801 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
     1861 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
     1921 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
     1981 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
     2041 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
     2101 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
     2161 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
     2221 atatcttggg aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
     2281 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
     2341 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
     2401 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
     2461 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
     2521 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
     2581 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
     2641 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
     2701 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
     2761 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
     2821 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
     2881 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
     2941 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
     3001 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
     3061 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
     3121 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
     3181 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
     3241 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
     3301 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
     3361 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
     3421 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
     3481 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
     3541 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
     3601 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
     3661 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
     3721 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
     3781 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
     3841 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
     3901 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
     3961 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
     4021 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
     4081 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
     4141 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
     4201 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
     4261 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
     4321 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
     4381 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
     4441 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
     4501 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
     4561 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
     4621 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
     4681 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
     4741 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
     4801 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
     4861 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
     4921 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
     4981 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
     5041 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
     5101 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
     5161 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
     5221 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
     5281 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
     5341 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
     5401 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
     5461 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
     5581 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
     5641 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
     5701 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
     5761 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
     5821 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
     5881 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
     5941 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
     6001 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
     6061 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
     6121 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
     6181 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
     6241 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
     6301 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
     6361 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
     6421 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
     6481 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
     6541 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
     6601 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
     6661 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
     6721 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
     6781 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
     6841 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
     6901 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
     6961 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
     7021 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
     7081 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
     7141 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
     7201 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
     7261 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
     7321 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
     7381 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
     7441 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
     7501 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
     7561 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
     7621 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
     7681 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
     7741 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
     7801 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
     7861 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
     7921 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
     7981 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
     8041 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
     8101 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
     8161 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
     8221 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
     8281 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
     8341 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
     8401 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
     8461 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
     8521 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
     8581 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
     8641 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
     8701 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
     8761 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
     8821 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
     8881 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
     8941 ggtaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
     9001 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
     9061 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
     9121 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
     9181 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
     9241 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
     9301 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
     9361 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
     9421 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     9481 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     9541 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     9601 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     9661 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     9721 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     9781 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     9841 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     9901 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     9961 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    10021 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    10081 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    10141 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    10201 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    10261 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    10321 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    10381 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    10441 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    10501 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    10561 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    10621 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    10681 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    10741 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    10801 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    10861 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    10921 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    10981 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    11041 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    11101 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    11161 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    11221 acaaacgatg ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg
    11281 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    11341 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    11401 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    11461 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    11521 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    11581 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    11641 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    11701 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    11761 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    11821 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    11881 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    11941 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    12001 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    12061 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    12121 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    12181 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    12241 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    12301 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    12361 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    12421 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    12481 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    12541 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    12601 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    12661 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    12721 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    12781 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    12841 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    12901 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    12961 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    13021 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    13081 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    13141 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    13201 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    13261 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    13321 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    13381 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    13441 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    13501 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    13561 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    13621 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    13681 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    13741 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    13801 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    13861 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    13921 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    13981 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    14041 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    14101 acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat
    14161 actacctcta ccacaagtga
//
LOCUS       DACCXM010000068        13896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCXM010000068 DACCXM010000000
VERSION     DACCXM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.070"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IVY61_001768"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IVY61_001768"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP7970882.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IVY61_001769"
     CDS             complement(1174..1869)
                     /locus_tag="IVY61_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP7970883.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IVY61_001770"
     CDS             complement(1882..2598)
                     /locus_tag="IVY61_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP7970884.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="IVY61_001771"
     CDS             2928..3536
                     /locus_tag="IVY61_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP7970885.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="IVY61_001772"
     CDS             3861..4184
                     /locus_tag="IVY61_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7970886.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="IVY61_001773"
     CDS             4366..5736
                     /locus_tag="IVY61_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP7970887.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="IVY61_001774"
     CDS             complement(5866..7167)
                     /locus_tag="IVY61_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7970888.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="IVY61_001775"
     CDS             complement(7521..9512)
                     /locus_tag="IVY61_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP7970889.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="IVY61_001776"
     CDS             complement(9656..9922)
                     /locus_tag="IVY61_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7970890.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="IVY61_001777"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="IVY61_001777"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP7970891.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="IVY61_001778"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="IVY61_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP7970892.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /locus_tag="IVY61_001779"
     CDS             13266..13829
                     /locus_tag="IVY61_001779"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7970893.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
BASE COUNT         3987 a         2901 c         2565 g         4443 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaag
//
LOCUS       DACCXM010000069        13712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCXM010000069 DACCXM010000000
VERSION     DACCXM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.071"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            174..512
                     /locus_tag="IVY61_001780"
     CDS             174..512
                     /locus_tag="IVY61_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970894.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(717..956)
                     /locus_tag="IVY61_001781"
     CDS             complement(717..956)
                     /locus_tag="IVY61_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP7970895.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(932..1279)
                     /locus_tag="IVY61_001782"
     CDS             complement(932..1279)
                     /locus_tag="IVY61_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP7970896.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2011..2592)
                     /locus_tag="IVY61_001783"
     CDS             complement(2011..2592)
                     /locus_tag="IVY61_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7970897.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2775..3401)
                     /locus_tag="IVY61_001784"
     CDS             complement(2775..3401)
                     /locus_tag="IVY61_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7970898.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3423..4754)
                     /locus_tag="IVY61_001785"
     CDS             complement(3423..4754)
                     /locus_tag="IVY61_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP7970899.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4909..5310)
                     /locus_tag="IVY61_001786"
     CDS             complement(4909..5310)
                     /locus_tag="IVY61_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7970900.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5396..5602)
                     /locus_tag="IVY61_001787"
     CDS             complement(5396..5602)
                     /locus_tag="IVY61_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970901.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5697..7199)
                     /locus_tag="IVY61_001788"
     CDS             complement(5697..7199)
                     /locus_tag="IVY61_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP7970902.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7217..7672)
                     /gene="argR"
                     /locus_tag="IVY61_001789"
     CDS             complement(7217..7672)
                     /gene="argR"
                     /locus_tag="IVY61_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7970903.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7770..8387)
                     /locus_tag="IVY61_001790"
     CDS             complement(7770..8387)
                     /locus_tag="IVY61_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970904.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8608..9981
                     /locus_tag="IVY61_001791"
     CDS             8608..9981
                     /locus_tag="IVY61_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7970905.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10056..11168
                     /locus_tag="IVY61_001792"
     CDS             10056..11168
                     /locus_tag="IVY61_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP7970906.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11240..12877)
                     /locus_tag="IVY61_001793"
     CDS             complement(11240..12877)
                     /locus_tag="IVY61_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP7970907.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13045..13653)
                     /locus_tag="IVY61_001794"
     CDS             complement(13045..13653)
                     /locus_tag="IVY61_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7970908.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3696 a         2887 c         2452 g         4677 t
ORIGIN      
        1 tagaaataaa ttgtagccag ttcatttttt ctttaactaa aaaaataatc caagaaacat
       61 tgaacctatg gatccaaaag ctacccaaat actatagcta accccccgct accttcaatt
      121 ttgcagataa aacgctttgg gagaaaattt caaagacaac aggccatcct atcatgcccg
      181 attataaaca aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca
      241 cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg cttcaaatag
      301 aaacattggt tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg
      361 tagctatttt tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat
      421 ggcaacctct tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga
      481 attttagatt ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa
      541 aagactcgca gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga
      601 ccctatagat gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga
      661 tctgtgctga agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat
      721 accttctcca ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata
      781 tctgttaccc aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt
      841 ggtgcgactg tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc
      901 tgatggctac gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat
      961 ttgtcttttt cttcattcca ctcattggga aaacacaacg attttgcaaa aatggttgat
     1021 gaaaggtctt tttttcttct actgtctctg cacgggcatt aatcatcaat tgtccttttt
     1081 taaatccggt aaatccccac ttcatgattt ccggagtgag gatgttttct tcctcttttc
     1141 ttaacgtcac aacaagattt gacggaaaaa cttctccagc tgctaattct gctgcctgct
     1201 tcggttgttt cgtctgggct tctcgataat aattctgaag gagctctgac tctagatcaa
     1261 aaaaataacg accacacatc ctaatccctc ccaatctttt tttctatcat acactgcaac
     1321 aagaagttcg caccaacaaa acgctttcaa tttttgtaga actaaaaaag ttgtgacaag
     1381 tgacaaactc tccagtgaat ggtattctcc tgccaactcc tcggtcacaa gtcacaatat
     1441 tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg tgactcggag tgaaacggca
     1501 ggcttacgac ctctgggtaa acggtgttca aaagccagtt tctcggtatt aagccaaatt
     1561 tatgcaaaaa tgtagaaagc tattttcaca aaatttttct taatactcga aactaagcga
     1621 ctttttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc
     1681 aaccaaacga aaaatatgag cagctatttt tatttggctg ttcttattac ttgatgcagg
     1741 gcagcttttt cacaacctct ccactaaacg gtgttcaatc agctaacctt cggtattagc
     1801 tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg tttctctaat actcaggtca
     1861 aagcgctgat ttcacaacct ctaatacacg gtgttccaga agtagcttct tcgaaaaata
     1921 agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
     1981 gctgaccact tctgtcacaa cctctaatta ttaaagaacc ttttgcgaag ttaacaatct
     2041 ttctttgatc aattgaaaat tctgcctaag tagtgataga tcctgtgtga ctttcaacaa
     2101 taagatactt ccctggattt cagcaatcaa cgctttggct tgacggtctg cttcttcatc
     2161 ggacagtttt tcttcagaaa gtgccaggat attttgtttc gtatacttga cccattgatt
     2221 gactaattct agtagcaaca gacggtattc ttcatccact gtactcaact caataatcaa
     2281 attacctaca ggacagccat gataagtgat ctgtgtctca tgatcagcga aaatccagtc
     2341 caacatttct tctaatgctt cttctggctg ttttccagca tttaagatgt cttcgaacaa
     2401 ttctgtctgc catacgtcca tatgcgcatg gatcaccgct agacaaactt ctttttttga
     2461 agaaaagtaa taataaaatt gaccttttcc tgtatcagaa gctgctagaa tatctcggat
     2521 tgacgtttca tgatagcctt ttttataaat aagatctttg gtcgcttcca agatcttttc
     2581 ctttgttttc aatgatattc tcctttttca tttctcatag tcctatatta tacagtaaat
     2641 tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc caaattatct gacacagccc
     2701 tcactataaa actgttctca tatagctaga tgttcattga ttcaagaact gcgaggacaa
     2761 aagcaattcc tatcttatga tagttgctct aatacttcct ctgttgtcat cacttgatct
     2821 gcaataaagc caaagttcgt caacgctgca tccattgctt cttggccagg agcaccaatt
     2881 gcatctgtcg ctaccacaac attgaatcca ctctctacca attcacgcat atgggaatcg
     2941 ctacacaagt ttccgttcat tccaccaaga attactgtgt cgattccatt ttttctcaat
     3001 tggagcgcca aatcattgct ttctggtcca aagattttat gaggagagct gatgatcgta
     3061 ttttcatcca aataaggttt caattcttcg ataaaatcgg ctcctgaacc ttcttccact
     3121 gattcatatt gactttttct agcaaacatc tgctgctcaa gcatcatatg ttctcctgca
     3181 ccgccaaatt gccatttatg gtcatgtggg taaaaataat gaggagaaat aaagagtttg
     3241 tatccttttt cttttgcttg ttttaccatt tcaatcatat tttcgatcgt attgtatttt
     3301 tctaagatat cttttgtcaa accaaagcca ccgcctgctg gtttcaagaa ctcgacttgc
     3361 gggtcagtga gaactactgc tgtattttta ttattgaaca tggtggattc tccattcgtt
     3421 atttatttta atacatcatt tagagcttca ctcattgtcg gatgagaata gatttgatct
     3481 ctcaataacg tataaggaag ttttccattc atagctagcg aaatcaagtt gatgacttca
     3541 tgagaatctt ccgcatagat acttgcccct agaatcaagt cagtttcagg gtcgactaaa
     3601 actttgaata cacctttagg atcttctaat acttgtgctt tagggacacc gcttgtcata
     3661 tacttgaata atttgtaatt gacgccttgt gctttggctt gtttttctgt taaacctaca
     3721 ttggataaag tcggagtgat aaatacactg taaggcacag ctttccggtt attcgttgtt
     3781 cgtttattct cgcccttcaa ctgatccata atgattcgat aatcatctag agagatataa
     3841 gtgaactgca atccgccttt gacgtctcca atcgcccaaa tgttgtctgc atctgtacga
     3901 aggtattcat ccacagcgat tgctccgcga tctgttagtt taacatctgt attttctaat
     3961 ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca aaacttttga cgcttggatt
     4021 gttacttctt ggccatcttc tgtaaaggtg acggcagctg ttgattcttg gtctgctact
     4081 tgatctacag atacacctag atggaattcg attcccgcat ttgtcatgtc ttctaaaatc
     4141 atttgcgcga tatcttcatc ttcacgtgga agaaattcag aatgggcatc aagaacgaca
     4201 actttagatc cgtattcatt gaacattgat gcaaattcca agccaatata tccagccccg
     4261 atgatcacta atttttccgg taattgttcc tgatccatcg cttgcgtaga atcaatcaag
     4321 tagcgacttg tattcaatcc tttgattggt aaaatgattg gttcagaacc agtattgatg
     4381 aaaatccgtt cgccttcaac aatcactttt tcaccattat tcgtcacttc gatttgatgg
     4441 ttagaaagaa aacgtgctgt accgtccatc actgtcccag ttgcctcatc gctgatcata
     4501 tgataatttt tggcgcgaag catgcccgtc aatttttctt tccgagcaac agcctctgta
     4561 aagtcgattc ctctttctcc attgaaaatc aatgatttag aaggaataca gccgatgttg
     4621 atacatgttc cgccatacat acgtgttgac ttttcaacga ctaaaaccga ttcacctttt
     4681 gaagctaacg ccttcgctaa agtcttaccg ccctttccaa aaccgatcac aatattttga
     4741 tatttttcca tcatttttcc ctcgcttctt ttttacctac cgattggtac aaaaatatga
     4801 taaccgataa ttgtctattc tccaaatcat ctgctttatt tgtcaaaaaa aaccagacaa
     4861 aaagcggtcc tttcacgttt ttagtctggt tcgatttcct ttattcttct aaacaatcgt
     4921 tacttctaca tcgatatttc ctctagttgc tttggagtaa gggcagaatt catgcgcatc
     4981 ttctgcaatt ttttgggctt cttcttcgct gatgccatcg atatgcactt ctaatactac
     5041 accgatctga tagccgccat catctaaatt ataaagagag acacgtgctt ttactgtgga
     5101 ctctgctgtc attcctgctt ctttgatcat atgttgtacc gccccattga aacaagaagc
     5161 ataacctgct gcaaataatt gttctggatt tgttccttct gcatggattc ctggaggagt
     5221 gattttcata tgcatcgagc catttggtgc ctcgacttct ccattgcgcc cgccatggtt
     5281 gatcattgct gtttcgtatg cttttttcat tgtactgctc ctttcgattg accatttgct
     5341 acttggctat aattatagtg tatgacaagt aaggcgcttt ctacaaaaat tctgcttatt
     5401 cgaataccag aatgccgtct tctactattt ttccaaaaaa caaaaaaata ctactcgtga
     5461 acaacagcgt catatttgtt ccaaaataaa tagcaatctt ttctacataa tcttttatat
     5521 gattgatttt aagatacata tataccagaa agttgacccc ataaaccaat actgttaaaa
     5581 aaataaccat attcgttacc attttttcct ccttttgctg gtctccctac cagcactcga
     5641 tttaaccata ccactttttt tgccgtccaa gcaactagcc tttcaaaaag actggtttat
     5701 tctgctgtta atgattcaag atcagaaaaa tgtccgaata attttgctaa ttttactaac
     5761 gccaacttgt tctttccgaa ttcatcattt tctatctgta atactagtaa cggctcatgt
     5821 aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct cttctagatc atatcgaata
     5881 ccttcttcat tttccgggtc gaagttcatc cctttttctt cttccaacag tcgcttcatt
     5941 tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc tgaacaattt taatcggatt
     6001 tcttgtactt ctacgggctg tttcaactct ttgatttgtt catctaatgt gatacctttt
     6061 ctttttaatt ctggcaaaag catcaggatc tttgcgatga catatgcacc atcatccaga
     6121 aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga tggctagctg tgtatctttc
     6181 ccaagacggt taagttctaa tgctttattg attacatttc gatagccgga aatataacga
     6241 acttggatgc ctcctttttc ttcaatgaat ctttgtagat gagaagaagt aggggaattc
     6301 gtcacgattc ttgttcctgg atgctctttt aatacgattg tacttaatac tgcgatcaaa
     6361 ttatttcgat tgatcactgt tccagaagca ctgacaactg cagaacgatc cacatctgta
     6421 tcgaaaatga tcccaagatc tgcttcattt gctaaaacag cctgcttgat actttccatc
     6481 gcttctttat tatcaggatt tgggatatga ttaggaaatc ggccatctgg ttccaaaaat
     6541 tgggaacctg ttgaatccgc acctaagcta ttcaatactt tttcagcaaa gaatccacca
     6601 gcaccatttc cggcatcaac aatgattttc caaccattta gtggtttgct ttctagtgtt
     6661 ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat aaggtgtcaa aaggtctgct
     6721 ttttctacgc ttccagtttg ttcgcttttt attttttgct gctgtgatag gataaaagca
     6781 atatcttctt tttctgctcc tccttctgag gtaaagaatt ttattccatt ataataatag
     6841 ggaagatggc tagcagtcag cataaccgca gcatcgcacg agaactgttc aaattgagtg
     6901 gacataaaca tagcaggcgt agtagctaat tcgaaatcga tgacatgtat cccttgttct
     6961 ttcagacttt ggatcaacgc ctctttcagt gctggtccgc ttatcctact gtcatgacca
     7021 atcccaatcg tccatttttt atttggatcg gttgatttct tctttttctt tatccagttt
     7081 gctagtcccc agccgatctt ttttaccatg tctgtcgtca agtttacttc atatccttca
     7141 actggtaatg cgattcctcg aatatccgat ccattttgca gcgctttcat atctttcata
     7201 taaaagtcct cctcatttat tccctcattt tttccagctt ttctgcaaat cgctgtgctt
     7261 cttcttctga gcggcaaata ataatgaatg tatctacacc ggccatcgtt ccgacaactt
     7321 cagggtaagc cacttcatct agccaattcg ttaccacatc ggcaccattt cgttcagtca
     7381 atacgataac catgaactga acagactgga tacgcaaaac ctcgcgtcta attgctgtac
     7441 gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa taatgtatag cgtactattt
     7501 tgttttcatt ttgacttttt actaacttca tttctcgaat gtctcgagaa atcgttgctt
     7561 gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag ctcttcctgt gtcccaatag
     7621 gttgttctgt aatgatctgg cggatcagtg cttgtcgctg tttctttttc atattagaca
     7681 cctctcaatg ttgattatag caaaagataa gtaatatttc ttcatatttt ggtatttttt
     7741 acttttattt tttgttttca ttcaggttgc taaccttttt ctattttaac acgcaaaatc
     7801 gtcttcattt tttcaggtgc tgattttctt ggatcgctga tgtaaatctc tttgtgttct
     7861 ttagaagctc tttcatagcc ttcttttgcg atgaatgctt ctaatttctc aaacgttgct
     7921 ggttcttcgg catagggccc gacgtgtaag atttgggcac aaagaccttc ttccatcttt
     7981 ttgaatacta cttgttcttt caagtcttcc ggaagtttgc tagaacgctg aatggcttct
     8041 tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca tcaattgata agaaaaatgt
     8101 tcttttttca tcactggttc atgcaaatac ttttcttgca gtccccattg tccttccagt
     8161 ggatagacag tatacatctg ataatcagga atcagccaat tttttttagg cgacatgcgt
     8221 attgcatagc tgactgcata tagacaggct actcttcgtt taaagtcttc ctgattcggg
     8281 tcgccgatcc ctctgatgct aaagaatgat tttttaggaa cctctagtat ttgcgggctt
     8341 tttttcatac tatagctctt gtctttacgc cattcaattt tttccatttt tcttcctccg
     8401 cgaaattcca tttagttatt tattatcata cctctttttg agtgacttgg ctaatcgtgt
     8461 tgacactatt tttcagtttg tgctagaata agtgaagtaa ataaatcgta cagataacac
     8521 gtttcggcgt gtcgagagag aggcggtttc tatttttata atagaaatca ctctcttttt
     8581 ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa aacagctgct tttagtacct
     8641 gcagtaattg caacagggat gatgtctttt gcgggcgtat tgatcgagac ggccatgaat
     8701 gtaactttcc caactcttat cagacagttt gatttaacga ctgcacaagt ccaatgggta
     8761 acaacgattt atcttttgat gatttcgatc attgttccgc tgtctactta tttgaatcgc
     8821 aattttagtc tcaaaaaact ctttttagct tcaaatctgc ttttcttggc aggtataatg
     8881 atcgatttct tctctcctac cttcatggtc ctattgtttg gtcgattgct ccaaggtgca
     8941 gcgacaggta tcgctcttcc gttgatgttc catattatct tgacatttgc ccccctcaat
     9001 cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg ccattgctcc agcaattggc
     9061 cccacttatg gaggcattat gacttcacat tttacatgga atcatatctt cctgtttctt
     9121 gtgcccgttt tgtttctttc tttgattatc ggctggtttg ctattcccga gatggcagta
     9181 caaaaaagtg gttatttgga ttggataagc gttttaggaa tttgtctttt gtttagtgga
     9241 agtctaacat ttttgagtac gttgggaaca gtttccagct ggattgcttt aatcatcggc
     9301 ggtattggct ggttcctttt ttactatcga agtaaaaaag tggagcatcc actgatccga
     9361 ctgtctattc tttcaaataa aacattccgc ttgtttttga ttggcttctt agtttatcag
     9421 tttttattac ttggtgtatc ttttgtcttg ccgaatttcc ttcagatcgt tcaaggaagt
     9481 agtccttttg tcgctggatt agcgatgctt cccggcgcag cggttggtgc tttgctttct
     9541 cctttttccg ggaaaatgct ggatcaatat ggtccgaaga agccaatttt tgctgggctg
     9601 attctttctt tgattggttg gctagcatta ttcctactca taggacacgc ttcagtgggt
     9661 gtattgattg cttgtcatgt gttttatatg atcggtatcg gattatctta cagcaatatg
     9721 atgaccaccg gaatgaatgc actgaatgaa gaactacaag gagacgggaa cgcaatgttc
     9781 aataccttgc agcagttttc tggcgctgtg gctacttcat tagtagcggt cattatcaat
     9841 tacgtccagc atcatacttc ccataactat gaagtgtcta ctacattagg atcaaaagca
     9901 gcattaggcg tcttgttgct gttgcttctc gtaagctttg ctcgattcgc ctattatctg
     9961 ttctttgcta aaaaagcgta gattccatat acggcaggtg ttgcatctgc cgtttttttg
    10021 tgtgaaaatg gttgtttgta aaaggaggct tttttatgga aaaaattgcc attatcggtg
    10081 gcgggatcat tggtatgacc ctagccagtt acttagatac atcaaaattc aatgttgctt
    10141 tatttgatga agggacagga caggcaacta aagctagcgc tgggattatc tccccttggt
    10201 tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa agacggcgct gcttttttcc
    10261 ctaaacttat aaaagatttc caacttgatc aaacgattta cgcacaatca gggacacttt
    10321 tgcttcgacc tgaaaaagat ttagctgaat tggctgttct tgccgagaaa cgaaaagaag
    10381 aagcgccaga aattggaaac atcgaatggc tttcacctga acagacatct caagcacttc
    10441 cgttattgaa accagcagtt tcactgcgta tcagtggtgg tggtcgtctg gatggaaaag
    10501 catatttaga acatcttggt cagcgtttag cacaaaaaca aatggagcag atccaggcaa
    10561 aagtcacttt gcataagaag agcgattctt ggctcatcaa gggaccgttt ggagaataca
    10621 cagctgatcg tgtaattttg acacctggcc cggcacttaa gcctttatta caagaattag
    10681 gctatgctgc agatatacgt ccgcaaaaag ggcagctctt agcatttcag acatcttata
    10741 agatgagtca agactggcct gttgctatgc ttgacggaga agctgatttc attcctttca
    10801 tggatggaaa gatcctttta ggtgctaccc atgaaaacga tcaaggctgg gatttggctc
    10861 ctactagcga agcttataag ggattgacta agagtacaga acagttcttg actgatccag
    10921 atcagataaa aaactggcca tacgagttcc gtgtaggtac ccgtgcttat acgtcagatt
    10981 tttccccatt tttcggcgta ctgccagatg atccaagctt actggctgct agcggtctag
    11041 gctcttctgg cttgaccaca ggtccttata tcggctatct attagccaac ttcttaaata
    11101 ctggtcattg gcctacggaa gattatcaaa aggacatctc cacctatatt acgaaaaatc
    11161 catcatagaa aaacagcttt ttcaagtaag gatcccgagc gatattgcgc atggattact
    11221 tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa attctccaaa ctttcttttg
    11281 cttcatctgg caagagatct tttcctatct gagcttcaat gaatcgtaaa cgaatatcaa
    11341 gtcgttgttt caccagattt ttataggacg tatcctcaaa agaaacagga aaatctggta
    11401 taggtaaata gtggatgtct tcaaatggac caaacgactt gaattctgcc gtttgttgca
    11461 cgattctttc aacgattgac aatgtcgttt caggtttgat ttttttatca tcgaaatatc
    11521 cagtattcag gacatagcag gctgtttgct gtttcaaaaa caactctcga aaagcttgat
    11581 agtcttccct aagaggatag acacggaaag gattagcaaa tggttcaatg actagctcat
    11641 ctagctgtct ttttgtagtg atattttccg cgtctgatcg ttttgttgct aatgttgcac
    11701 caaaagacgc tgctaaaact gggtcattga tcttgataat tggcggaagc gaatcatctt
    11761 tcatgatcca aaaaacagca tcgatctttt cgctaagatg atcgactcgg tcgggtgtcg
    11821 cataacgtga tttgactgtc cgcccatttc cgtttcgtat atctcctgtt actaggactt
    11881 tttctccttt acttgtacat gtcacgccca cattctgaca agtaaggaaa gaagaaatgg
    11941 ccggatcatc agacggatag tcttgtgttt tatcaaagta agaaggttcc aaggcagtag
    12001 caaaaccatt ttttcgagaa ataacgaaag catcgtcatg caagatcgaa acagagtgac
    12061 cttccgtatg ttttgctaag gtaatcgttg attttccaga accggacaac ccaaatacag
    12121 ccatcgtata ggtctggtca ttactagcat attgtttcat cccaccgtgg caggcaataa
    12181 agccattgcg atttcccaat gcccaagcaa gcgtcaaagt cgcttttttt aattctccaa
    12241 agtaacgtaa tcctagcaca gccgctgtat tatggagagc atcaaacaaa atgagtccat
    12301 ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat ataaagatct ccctcatcaa
    12361 aggaaacaga cttttgataa gtctctaaga tcttcgaatt cgctggctga aaattcagca
    12421 aataagaata caaattgttc tcaaatcctt ctggaatcag caaatgactt tgcacagaaa
    12481 attcttcaga taggccaacg attacttttg tatggtaaaa ctggcgcttg cgactttgat
    12541 acacagcttc tcttaaaatt ttctcgtatt tgctttgttc cttgtctaag tcctcaaaga
    12601 tccgccgagc acttgctgtt cttcccacaa tgcttccatc attgaatact agtgtcctaa
    12661 catcagctgg taaatctagt tcttcagcat gtgagattgt taaatctgtg atgatcgttc
    12721 ctggactccc ttttgctagt tgataagctg tcttcctatc aaaaacttca tgaacattat
    12781 taccgtaaaa tgtcgtttct acaatcgatc gaatactgga taacagtgga tttccacttg
    12841 tgatttcgtt taattcgaga ctctcttttg tactcatggc tcctcctcat tccctaaaca
    12901 aaaaaacgtt ttctttatta tacattattc gagtttttat ttgtactgct gcgctgtggg
    12961 aagcaaacga gttatttttc acacaaaaaa accgagaact tgaataaaca agtttctcgg
    13021 tttcatcatt ttccttttat cggcttagta ccagccgttt gctaaccaga atgttttagc
    13081 agcatcccat gaaccataac gagatgttac atattgttct gctacacgtt cttgattggc
    13141 agctgaatag tcgccgttta agtatgaagc atctaattga taacggccga tgtaacgtcc
    13201 gttagtagct gtgtatgaac cgcctgattc tttttgtgcg atccattctt ttgcagagct
    13261 tgaagttggc gctgttgcag cagctggggc agcaggagct acttcaactg tttcaacagg
    13321 tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta gatgctgtat tttctgttgt
    13381 tttgttttct gttgttgctg aagaatcatt tgaagttggg attgttaatt gttcaccaac
    13441 ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca attgcatcga tcattgagtt
    13501 gtctcctgca aatttttgtg agatcttaga taatgaatca cctgatttta ctgtataaac
    13561 ttcatcagcg tgagcatttg tagttcctac aaacattgca gtagcaccag cagcaaaagt
    13621 tgtacctaaa acaattttct taaatgaatt catattaaac tccttttttt ctccgttttt
    13681 tattctttcg acgctattaa ctttatcatt ta
//
LOCUS       DACCXM010000070        13696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCXM010000070 DACCXM010000000
VERSION     DACCXM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.072"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            496..1098
                     /locus_tag="IVY61_001795"
     CDS             496..1098
                     /locus_tag="IVY61_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7970909.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1114..1791
                     /locus_tag="IVY61_001796"
                     /pseudo
     CDS             1114..1791
                     /locus_tag="IVY61_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2303..2599
                     /locus_tag="IVY61_001797"
     CDS             2303..2599
                     /locus_tag="IVY61_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP7970910.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            2600..3016
                     /locus_tag="IVY61_001798"
     CDS             2600..3016
                     /locus_tag="IVY61_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP7970911.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3036..4580
                     /locus_tag="IVY61_001799"
     CDS             3036..4580
                     /locus_tag="IVY61_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7970912.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4723..4947
                     /locus_tag="IVY61_001800"
     CDS             4723..4947
                     /locus_tag="IVY61_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7970913.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            4962..5831
                     /locus_tag="IVY61_001801"
     CDS             4962..5831
                     /locus_tag="IVY61_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7970914.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5812..6546
                     /locus_tag="IVY61_001802"
     CDS             5812..6546
                     /locus_tag="IVY61_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7970915.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6579..7487
                     /locus_tag="IVY61_001803"
     CDS             6579..7487
                     /locus_tag="IVY61_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7970916.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7484..8026
                     /gene="sat4"
                     /locus_tag="IVY61_001804"
     CDS             7484..8026
                     /gene="sat4"
                     /locus_tag="IVY61_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP7970917.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8119..8913
                     /locus_tag="IVY61_001805"
                     /note="aph(3')-IIIa"
     CDS             8119..8913
                     /locus_tag="IVY61_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7970918.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(9021..9209)
                     /locus_tag="IVY61_001806"
                     /pseudo
     CDS             complement(9021..9209)
                     /locus_tag="IVY61_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9189..9761
                     /locus_tag="IVY61_001807"
     CDS             9189..9761
                     /locus_tag="IVY61_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7970919.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            10101..10955
                     /locus_tag="IVY61_001808"
     CDS             10101..10955
                     /locus_tag="IVY61_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7970920.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERI
                     IQLENEKE"
     gene            11054..11263
                     /locus_tag="IVY61_001809"
     CDS             11054..11263
                     /locus_tag="IVY61_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7970921.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11280..11552
                     /locus_tag="IVY61_001810"
     CDS             11280..11552
                     /locus_tag="IVY61_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7970922.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            11554..12417
                     /locus_tag="IVY61_001811"
     CDS             11554..12417
                     /locus_tag="IVY61_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7970923.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(12680..13417)
                     /gene="erm(B)"
                     /locus_tag="IVY61_001812"
     CDS             complement(12680..13417)
                     /gene="erm(B)"
                     /locus_tag="IVY61_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7970924.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(13542..13625)
                     /locus_tag="IVY61_001813"
     CDS             complement(13542..13625)
                     /locus_tag="IVY61_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP7970925.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         4853 a         2128 c         2848 g         3867 t
ORIGIN      
        1 tatgatacgt gtttgttttt ctgtgctgtt tagtgaatga ttagcagaaa tatagagagt
       61 aagattttaa ttaattatta gggggagaag gagagagtag cccgaaaact tttagttggc
      121 ttggactgaa cgaagtgagg gaaaggctac taaaacgtcg aggggcagtg agagcgaagc
      181 gaacacttga tcttttaagt tgctatcttt ttaaggtcaa tagagtatac ttatttgtcc
      241 taatatggtt ttagcagtat aattgacttg gtgaataggt catttaagtt gggcataata
      301 ggaggagtaa aatgaaaaaa tttatttatc gagttttaga aaatgacgaa gtggtggcta
      361 tttttaatga gcaacaatat gcgcaagatt ttatcgctta cgaaaagaca atttctgata
      421 agcaatttga aattgaaaaa gtagatatag ctgattggtt attgcaaccg agagaatttt
      481 agaggttggt tgaaaatggc taaaattggt tatgcacgtg tcagtagcaa agaacagaac
      541 ttagatagac aactgaaagc gttagagggc gtttctaagg tcttttcaga caaattaagc
      601 ggtcaatcgg tcgaacgccc acaattacaa gctatgctta actatattcg tgaaggggat
      661 atagttgttg ttactgaatt agatcgatta ggacgaaata ataaagaatt aacagaattg
      721 atgaatcaaa ttcaaattaa gggggcaacc ctggaagtct taaatttacc ctcaatgaat
      781 ggtattgaag atgaaaattt aagacggctg attaataatt tagtgattga attgtataag
      841 taccaagcgg aatctgaacg caaacgaatt aaagaacgcc aagcccaagg aattgaaatt
      901 gctaagaaaa aaggaaaatt caaagggcga caacctaaat tcaaagaaaa tgatccacgt
      961 ttacaacacg ctttcgattt atttttgaat ggttgttccg ataaagaagt tgaagaacaa
     1021 actggaatta atcgccgaac gtttagaagg tatcgatcaa gatacaacgt gacagtagat
     1081 caaagaaaaa aacaatgaaa agagggatag ttaatgagta cggttatttt agctgaaaaa
     1141 ccaagccagg cattagccta cgcaagtgct ttaaaacaaa gcaccaaaaa agacggttat
     1201 tttgagatca aagacccact atttacagat gaaacgttta tcacctttgg ttttgggcat
     1261 ttagtcgaat tagcagaacc aggtcattat aacgaaaagt ggcagaactg gaaacttgaa
     1321 tctttgccga tttttcctga tcgatacgat tttgaagttg caaaagataa gggaaagcag
     1381 tttaaaattg ttgctgaact tctcaaaaag gcaaatacaa ttattgtcgc aacagatagc
     1441 gacagagaag gtgaaaatat cgcctggtcg attatccata aagcaaatgc cttttctaaa
     1501 gataaaacgt ataaaagatt atggatcaat agcttagaaa aagatgtgat ccgaagcggt
     1561 tttcaaaatt tgcaaccagg aatgaattac tatccctttt atcaagaagc gcaaacacgc
     1621 caaattgccg attggttgat cggcatgaac gcaagccctt tatatacctt gaatttacaa
     1681 caaaaaggcg tacaaggtac attttcatta ggacgtgtac aaacaccgac tttatatctc
     1741 atttatcaac gccaggaagc aatagaaaac tttaaaaaag aacctttttt cctgaataat
     1801 tcatagtttt actaatagtc tttcctaaca ttgatttaat agtctttaaa gtattctgct
     1861 tcatcaccca ttaggtgatg aaaaaagaac ccctttctga tttagcggac aaatactttg
     1921 acctaatctc agaagatata aaagaaaaaa taataaaata tccgatttat actttgtata
     1981 aagaactaaa gtagttagca aatcatttac agcaaatcac tatgttccac ataccatttt
     2041 acatacttac cttatccacg acacatgttg agatagtatc gaagataaag aaagttgctc
     2101 catgttgttt ttgcgagatg gggaaatcaa agccacattg acaaccatga tgaataagct
     2161 tgctttcagt cataagctaa tcctagaacc acttttcaag tcagttagcc aaatcgatga
     2221 agaaagcgac cgtgaaagaa tggacgctat tgataagcta atggagcaac tattggaaga
     2281 acgcaatacc cttattgcac tgatgtccaa aggtttccta gaacctgctc tttttaatca
     2341 ggaacgaaat gttttagata gtgagattaa aaatcttact accgaaaaaa caaacctggt
     2401 aacgaactct gcgagtgggg ttttgcgagc aaacgagata aaggacctca ttaattatgt
     2461 gtcagcagat aattttaatg gtgactacac ggaagaacta tttgaagaat ttgtagtgaa
     2521 catcattgta aattccaggg atgagctgac attcaatttg aaatgcggtc tttccctgaa
     2581 agaaaaggtg gtgagataaa tggcatatat tccatatgga tacaaaattc aagatggagt
     2641 ggttactgtc gatgaaaagg cagcaggtca agtaaaggta ttctttgaga aatacatatc
     2701 aggactatcc cttacagtgg ctggcgaaca ggcaggtatt gaaaagacac attctgtgat
     2761 ggggcgcatt ttgaaaaacg tcaactacct tggaaatgat acgtatccag caattattga
     2821 taaagagata tttgataaag ctgaagaagt tagagataaa cgtgcaaagg atttaggacg
     2881 agtggtagag cttgccgctt tcacctctcc ccctcccaaa gaacgattca aaatgaaaaa
     2941 ggcagataat aagatgccag tggatccttt tgaacgagca gaatacttat acagtctgat
     3001 agaaagcgag gaataaagtg acagagaaaa atataatggt tattcctgct cgtaaaagag
     3061 taggaagtac agccgcaaaa gaaaagataa agaaacttcg tgttgctgcc tattgccgtg
     3121 tttctacaga aacagaagaa caaaattcta gctatgaggt tcaggtctca cactatacag
     3181 agtttataaa gaaaaataat gaatgggagt ttgctggcat ctttgcagat gatggtatct
     3241 ctggtacgaa cactaaaaag cgtgacgaat ttaatcgcat gattgcagag tgtatggacg
     3301 gtaacatcga catggttatt acaaaatcca tcagccgatt tgcgcgtaac accctagact
     3361 gccttcaata cattagacag ctcaaggata agaacatatc agtttatttt gagaaagaga
     3421 acatcaacac cacggatgcc aaaggtgagg ttttgctgac tattatggca tctttggcac
     3481 agcaggaaag ccagagcctt tcacaaaacg ttaagcttgg gctacagtac cgataccaac
     3541 aaggaaaggt gcaggtcaac cataagcgat ttatgggcta cacgaaagat gaagatggaa
     3601 atttaactat tgtccccgaa gaagctgaga ttatcaaacg catctaccga gaataccttg
     3661 agggtcagag tctagtgggc attggtcgag cccttgaaaa ggatggtatt ttaacagcag
     3721 cgggaaaacc aagatggcga ccagaatcag ttaagaagat acttcaaaac gaaaaataca
     3781 tcggagatgc ccttctgcaa aagactgtca cagtagattt tctgaccaag aaacgagtga
     3841 aaaatgaagg gcatgttccc caatattatg ttgaaaatag ccatgaagcg attattccta
     3901 aagagttatt tttgcaggcg caagaagaaa ttcatcggag aagcaatatc tacacaggag
     3961 aaggcaagaa caaaagaatt tacagtagca agtacgcttt aagtgccatc accttctgtg
     4021 gagattgtgg cgatatttat agacgggtct attggaatat ccacggaaga aaagaatttg
     4081 tctggcgatg cgtgactaga atcgagcaag gtcctgaagt ttgtaagaac cgaaccgtaa
     4141 aagaagatga actttatggt gcagtaatga ctgcgattaa taagctactt gcaggtggga
     4201 acaatatgat aaagacactg gaagagaata ttcatgctgt gattggcgaa accacagaat
     4261 accaaatttc agagattaac aactcactag aggaaaaaca aaaagaacta atcaagctgg
     4321 cgaacaaggg tcaagactat gaccacttag cagatgagat tgatgagctg agagacaagc
     4381 gacagatcct tttagtagaa gatgcctccc tcagtggcga gaacgagcga atcaatgagc
     4441 tgattgaatt tatccgcaag aacaaattcc gcaccttaga atatgatgat aagcttgtaa
     4501 ggaagataat ccagagcgtt acagtctatg aaggccactt cgtcatagcc ttcaaatctg
     4561 gcatcgaaat ggaaatatga aaaccagaag taaatagccc atgactctac agtagagttg
     4621 tgggttttct tttgcttgct gattataata atattttaat aactattgtg tttatcaacc
     4681 aaatggttta taatattctt agcgtaaact tggaggtgct gtatgactac ggcagaaatg
     4741 attaaagaac tgtgtgagca aatgaatata agtgtttccg aacttgctag acgtattggc
     4801 cagactccac agaatttcaa taaaaaatta caacgagaaa cagtaacctt ggatgagttg
     4861 aaggccattg ctgatgtgct aggtgtcaag tttgtgcagg catttatttt accagatgga
     4921 gaagaaataa aaatatctaa cgaataaagg aggatgttta tatggtaggt aatgttattc
     4981 aaatagtaac agagaaatta tcatctttgc cttttataga aggcatcgta ttagggggct
     5041 cacgtgcaag aggcacccat acagaaaatt ctgatataga tatcggaatc tattacaatt
     5101 cagactcatt tgacctgaca gcgattaatc aaattgctac agagttggat gatgagaata
     5161 gaaacaacct tgttgtacct cccggagcgt ggggtgattg ggttaatggc ggtggatggt
     5221 tagttataaa cgggtgtcat gttgacttga tattacgtga tataaaacgg gtggaacaaa
     5281 taatcaaaga tacggagcaa ggaattgtta ctgccaatta tcagaccggg catccccatg
     5341 gttatataag tgctatgtat cgtggagaat tagcgattag taaaatacta tatgctaaga
     5401 acgaaagctt atgcgaatta aaaaagcagg cagaaattta ccctactgct ctaaagaaaa
     5461 gcttgatgaa cttttttata tttgaagctg aattctcttt aatgtttgta aaagcaaatg
     5521 cgggggcaga ggataaatat tatattgcag gccatgtttt tcgtattatt tcatgcttaa
     5581 atcaagtact atttgcatgt aataatgctt attgcattaa cgaaaagaaa gctataaaac
     5641 tgcttgaaac ttttgaatat aaacctgaaa aatatgccga aagagttaat catatttttg
     5701 aagtactcgg tctttctctt tttgaatgct acgacatgac cgagaaactt tataaagaag
     5761 tgaatgaaat tgtatcggag ataaataact ttttaaacga ggagagttca gatgaaagaa
     5821 aacaaatatg atgataatat attttttcaa aaatacagtc aaatgagtcg ctcgcagaaa
     5881 ggactggctg gtgcgggaga atgggagact ttgaaaaaga tgctacctga ttttaagggt
     5941 aagcgtgtgc ttgatttagg atgcggctat ggatggcact gtatatatgc gatggaaaac
     6001 ggtgcttcct ctgtagtagg tgttgatatt tctcataaaa tgctcgaagt agcaaaagga
     6061 aaaacccatt ttccacagat tgaatatgaa tgctgtgcca tagaagatgt ggatttccca
     6121 gaggagagct ttgatgtaat actaagttcg cttgcgtttc attatgtagc agactatgag
     6181 aatttaataa aaaagatata taggatgctg aaggctggtg gcaatttagt ttttacagtt
     6241 gaacatcctg tttttactgc tcatggaaca caagactggt attataacga aaaaggagaa
     6301 atactgcatt tcccggtgga caattattat tatgagggca aacggacagc tatgtttttg
     6361 gaagaaaagg ttacaaaata tcatagaaca ctgaccacat atctaaatac actgctttca
     6421 aatagtttta taataaatca gattgtggag ccacagccgc cagagaacat gatggatatt
     6481 ccggggatgg cggatgaaat gcgacgccca atgatgctga ttgtatcggc aaaaaagaag
     6541 atgtaataat atagaaaaaa taaacgagga gtatgtaaat gagatcagaa aaagaaatga
     6601 tggatttagt actttcttta gcagaacagg atgaacgtat tcgaattgtg acccttgagg
     6661 ggtcacgcgc aaatattaat atacctaaag atgaatttca ggattatgat attacatatt
     6721 ttgtaagtga tatagaaccg tttatatcta atgatgactg gcttaatcaa tttgggaata
     6781 taataatgat gcaaaagccg gaggatatgg aattattccc acctgaagaa aagggatttt
     6841 cctatcttat gctatttgat gattacaata aaattgatct taccttattg cccttggaag
     6901 agttagataa ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta
     6961 gaattaaaag ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa
     7021 gggagtatga tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat
     7081 tgtgccgtaa ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc
     7141 tgctgagaat gatatcatgg aaggtcggca tcgaaacagg ctttaaatta agtgtaggca
     7201 agaactataa gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca
     7261 cctaccggat ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt
     7321 tcagggcggt atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata
     7381 ggaatataac aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc
     7441 tggatagcac atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa
     7501 agcagggcac ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat
     7561 tataccgagg tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata
     7621 tttaaaaagc taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata
     7681 tattgacaat actgataaga taatatatct ttactaccaa gacgataaat gcgtcggaaa
     7741 agttaaactg cgaaaaaatt ggaaccggta cgcttatata gaagatatcg ccgtatgtaa
     7801 ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa
     7861 gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa
     7921 attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
     7981 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
     8041 tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa
     8101 agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
     8161 gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
     8221 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
     8281 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
     8341 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
     8401 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
     8461 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
     8521 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
     8581 gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt
     8641 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
     8701 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
     8761 gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa
     8821 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
     8881 aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt ctaaaaagct
     8941 ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa ctgtccatac
     9001 tctgatgttt tatatctttt ctaaaagttc gctagataga gttctatatt agagatgtaa
     9061 agagttttgg tgaagaggtt tgtgagtatg gcttttataa ccagggtgca atgagaagca
     9121 ctaacagcag ctagcttaag aacgcatagg atatactttt tatggaagtc acagaggagg
     9181 aataaaattt gaacaggatt aacagagtaa cctctattct tattcagctg caatcaaaaa
     9241 aaataattcc tgccaaagaa attgcacagc gatttaatat aagcttaagg acagtttaca
     9301 gagatatccg gacacttgaa gaagccggaa taccaattgg atctgaggcc ggaaaaggat
     9361 attttcttgt agagggcttc ctacttccgc cggtaatgtt tacagcggcg gaagtgggtg
     9421 cattgattac agcagggaaa tttttaaatt gccatggaga tgaatcattt ataaaggatt
     9481 ttgattcagc tatgtataaa atcaaatcta ttttgaagca tggtgaaaaa aactatgcac
     9541 aggagttgga gaacagtatt aacgtgtaca gcacatctgg acagaaaaat acattggcgg
     9601 ataacgttat tgcagcaatt cagactgcaa tctgcaataa aagggtaata tcaattcaat
     9661 atcctgcatc aggagggcaa gaaccggaaa gcagaatgat agagcctata tcactggggt
     9721 tttatgaaca gaactggtac ttaatcggtt ttgcaggcta agaaatgaat ataggaattt
     9781 tcgtgtggat cggattaaaa gcatttgcat agaagaagag ctctgtcaaa gccatgatgg
     9841 ctctttggaa caaatattaa aacaaatgct ttcatataaa aaactatatg ccctacaatg
     9901 ccgttgtaaa aattggggaa aagggatata ttgattttat atctttctca aaataaatat
     9961 aaaagccctt taaagagggc ttttatatat taatcacaaa tcacttatca caaatcacaa
    10021 gtgattaatc acaaatcact tgtgatttgt gattcttaat gatacaatat tactatacaa
    10081 aaaaagaatg gggcgtagtt atggagaagg aaaaactaaa aatacttgaa gaattaagac
    10141 gtattttaaa caataaaaat gaagcaatta ttatcttgaa taattacttt aaaggtggtg
    10201 ttggaaagtc caaattatcg actatgtttg cttacttgac agacaaattg aatttaaaag
    10261 ttttaatgat cgataaggac ttacaggcaa cattgacaaa agacttagca aaaacttttg
    10321 aggtagaatt gccacgtgtc aatttttatg aaggcttgaa aaatggaaac ttggcttctt
    10381 ctattattca tttgactgat aatttagact tgatccctgg cacgtttgat ttgatgttac
    10441 tgccaaaatt aactcgctca tggacgtttg aaaatgaaag tagattgctt gctactcttt
    10501 tagcaccttt aaaaagtgac tatgatctta ttattattga tactgtacca acgccaagcg
    10561 tttatacaaa taatgcaatc gtggcgagtg attacgttat gatcccttta caagcagaag
    10621 aagaaagtac aaacaacatt caaaactata tttcctattt gattgatttg caagaacagt
    10681 ttaaccctgg actagatatg attggttttg ttccttattt agttgatacg gacagcgcaa
    10741 cgataaaatc aaacctggaa gaactgtaca aagaacataa agaggataat ttggttttcc
    10801 gaaatattat caagcgaagt aataaagtaa gtacttggtc taaaaatggc attacagaac
    10861 acaaaggcta tgacaaaaaa gttttgtcta tgtatgagaa cgtatttttt gaaatgattg
    10921 agcgaatcat tcaattagaa aatgaaaaag aatagaatca caaatcacaa gtgattaatc
    10981 acaaatcact tgtgatttgt gataggtgat gataaaataa atagtaagaa gaaatagaaa
    11041 gaagtgagcg atcgtgggaa atttaggcgc acagaaagca aaacgaaatg atacgccaat
    11101 cagcgcaaaa aaagatataa tgggggataa gacggttcgt gttcgtgccg acttgcacca
    11161 tatcataaaa atcgaaacag caaagaatgg cggaaacgta aaagaagtta tggatcaagc
    11221 cttagaagaa tatatacgga aatatttacc tgacaaactt taaaaggagt gaaaatgaaa
    11281 tggcagttac gtatgaaaaa acatttgaaa ttgaaatcat taatgagtta tcggcaagtg
    11341 tttataatcg ggtattaaat tatgttttga atcatgaact agatactaaa aatactcgtt
    11401 tactagaagt gaatctttta aatcaattag aagtggcaca agaagttgat ttatttcaac
    11461 aaccatttga agaattacaa gctattcatg agtattggcg gtcaatgaat caatattcaa
    11521 aacaaatttt gaataaagag aaagtggctt aacatggcga atattactga cttcaccgaa
    11581 aagcaatttg aagatcgttt agaaaagaat gttgaacgac taactaaaaa tagactagcg
    11641 gttgaatcgc caaccgcttt tttacttggt gggcaaccag ggtcagggaa aaccagtttg
    11701 cgatcagcaa tttttgaaga aacacaaggg aatgttattg tcattgataa tgataccttt
    11761 aaacaacagc acccaaattt tgacgaatta gtgaaactct atgaaaaaga cgtagtaaaa
    11821 cacgttaccc cttattcgaa tcgcatgaca gaagcactta ttagccgttt aagcgatcaa
    11881 ggctataatt tagtgattga aggaacagga cgaacaacag acgttcccat tcaaacagcc
    11941 acaatgcttc aatctaaagg ttatgaaaca aaaatatacg tcatggcagt accgaaaatt
    12001 gaatcatact taggaacgat tgaacgatat gaaaccatgt atgcagatga tccaatgaca
    12061 gctagggcaa caccaaaaca agcgcatgat attgttgtca aaaacttacc gaccaattta
    12121 gaaacccttc ataaaacggg cttatttagc gatataaggc tttacaatag agaaggagtg
    12181 aaactctatt caagcttaga aacgccttcc attagtccga aagagacctt agaaagagaa
    12241 ttaaatcgta aagtatcagg gaaagaaatt caaccgactt tggagagaat agagcaaaaa
    12301 atggttcaaa atcaacacca agaaacccct gaatttaaag caattcaaca aaaaatggaa
    12361 agcttgcagc tacctacacc accaataccc aaaacaccta aacttccagg tatttaaacc
    12421 ttaaaaaagg aaaagagtag ttaccaaaaa acggtaacta ctcttttttt ataaaaacat
    12481 ttcctcatgt tggggtcccg agcgcctacg aggaatttgt atcgataaga aatagattta
    12541 aaaatttcgc tgtttttttc taatttcatt ttgaggtgaa ttaattggta gttattagag
    12601 gtgccctata cctaataatt tatctacatt ccctttagta acgtgtaact ttccaaattt
    12661 acaaaagcga ctcatagaat tatttcctcc cgttaaataa tagataacta ttaaaaatag
    12721 acaatacttg ctcataagta acggtactta aattgtttac tttggcgtgt ttcattgctt
    12781 gatgaaactg atttttagta aacagttgac gatattctcg attgacccat tttgaaacaa
    12841 agtacgtata tagcttccaa tatttatctg gaacatctgt ggtatggcgg gtaagtttta
    12901 ttaagacact gtttactttt ggtttaggat gaaagcattc cgctggcagc ttaagcaatt
    12961 gctgaatcga gacttgagtg tgcaagagca accctagtgt tcggtgaata tccaaggtac
    13021 gcttgtagaa tccttcttca acaatcagat agatgtcaga cgcatggctt tcaaaaacca
    13081 cttttttaat aatttgtgtg cttaaatggt aaggaatatt cccaacaatt ttatacctct
    13141 gtttgttagg gaattgaaac tgtagaatat cttggtgaat taaagtgaca cgagtgttca
    13201 gttttaattt ttctgacgat aagttgaata gatgactgtc taattcaata gacgttacct
    13261 gtttacttat tttagccagt ttcgtcgtta aatgcccttt acctgttcca atttcgtaaa
    13321 cggtatcggt ttcttttaaa ttcaattgtt ttattatttg gttgagtact ttttcactcg
    13381 ttaaaaagtt ttgagaatat tttatatttt tgttcatgta atcactcctg aagtgattac
    13441 atctataaat aaatacagaa gttaaacgat ttgtttgtaa ttttagttat ctgtttaaaa
    13501 agtcataaga ttagtcactg gtaggaatta atctaacgta tttatttatc tgcgtaatca
    13561 ctgtttttag tctgtttcaa aacggtagat gttttatcta cattacgcat ttggaatacc
    13621 aacatgacga atccctcctt cttaattaca aatttttagc atctaattta acttcaattc
    13681 ctattataca aaattt
//
LOCUS       DACCXM010000071        13344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCXM010000071 DACCXM010000000
VERSION     DACCXM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.073"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            15..161
                     /locus_tag="IVY61_001814"
     CDS             15..161
                     /locus_tag="IVY61_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP7970926.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(223..2034)
                     /locus_tag="IVY61_001815"
     CDS             complement(223..2034)
                     /locus_tag="IVY61_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP7970927.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2031..2396)
                     /locus_tag="IVY61_001816"
     CDS             complement(2031..2396)
                     /locus_tag="IVY61_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP7970928.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2559..2954)
                     /locus_tag="IVY61_001817"
     CDS             complement(2559..2954)
                     /locus_tag="IVY61_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7970929.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2972..3934)
                     /locus_tag="IVY61_001818"
     CDS             complement(2972..3934)
                     /locus_tag="IVY61_001818"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP7970930.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3934..4146)
                     /locus_tag="IVY61_001819"
     CDS             complement(3934..4146)
                     /locus_tag="IVY61_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP7970931.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4167..4865)
                     /locus_tag="IVY61_001820"
     CDS             complement(4167..4865)
                     /locus_tag="IVY61_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7970932.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4885..5427)
                     /locus_tag="IVY61_001821"
     CDS             complement(4885..5427)
                     /locus_tag="IVY61_001821"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP7970933.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5559..6563)
                     /locus_tag="IVY61_001822"
     CDS             complement(5559..6563)
                     /locus_tag="IVY61_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7970934.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6560..7549)
                     /locus_tag="IVY61_001823"
     CDS             complement(6560..7549)
                     /locus_tag="IVY61_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7970935.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7546..8352)
                     /locus_tag="IVY61_001824"
     CDS             complement(7546..8352)
                     /locus_tag="IVY61_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7970936.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8518..9474
                     /locus_tag="IVY61_001825"
     CDS             8518..9474
                     /locus_tag="IVY61_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7970937.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9551..10069)
                     /locus_tag="IVY61_001826"
     CDS             complement(9551..10069)
                     /locus_tag="IVY61_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7970938.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10157..10306)
                     /gene="rpmG"
                     /locus_tag="IVY61_001827"
     CDS             complement(10157..10306)
                     /gene="rpmG"
                     /locus_tag="IVY61_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7970939.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10534..10980
                     /locus_tag="IVY61_001828"
     CDS             10534..10980
                     /locus_tag="IVY61_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7970940.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11175..13070)
                     /gene="asnB"
                     /locus_tag="IVY61_001829"
     CDS             complement(11175..13070)
                     /gene="asnB"
                     /locus_tag="IVY61_001829"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP7970941.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4152 a         2831 c         2276 g         4085 t
ORIGIN      
        1 aatttgtatc tggaatatta gtaggaagtc tggcaacagc cgctgccgtt gctggtttgg
       61 ttgcttctgt gaaaaaaaca gtgatcgatc caattgacga aaaagaagca atgatcgaag
      121 aaaaccgtaa aaaagcaatg cgtaaacgcg tttctcgtta ataattattt aaaaatacgc
      181 atgaagactt tctaaaaaac agataagtca tcatgcgtat ttttatttgt cagtcaatat
      241 aagttgtgct attttatctg cctgtctcag attccatgga tctgtataag gtctagctac
      301 tgctgcagtt agccaatcca caccttcaac aggtattcca tagcaataaa agtcagactg
      361 gatttcccct gaagcatcaa tcacgcgatt cgtacctcgc tcaacatcta tagcttctga
      421 atgataactt cctgtattat ctgaaaatga aaaagggcga acaagtttgt tttccttcaa
      481 attttttatg acaggattac ttgaacgaga aaagcttgtt tgtggcaatc gagcttcaat
      541 caaatagcga ctgctgattt ctttatcttg tagagaaata acatagcggt tgtgcttttg
      601 ctgaatatca aatggcggtt cgatcaaatg aaaaataccc gcatctatca gtgctgctaa
      661 ttctttcgtc cgaatgattg gcggaccgat cgttaaaaag ctatttagat gagtaaacca
      721 tccccatagt atttctttgt attccttagc agaaaaaacg tgccattcaa tagcctgccg
      781 gattggatca cgccagtctt ttaatgcatc aaatgttgaa gcaaatgctc ctgtgcaatt
      841 gcctttttcc gcttctttta tctgcccttc aataaatatt cggctagctt ctgaaaaggg
      901 ctcattctga taggcaagag gagtttctaa atacgaccaa tcccatctat aagggattag
      961 tgctggatgc tttccgagac actcctctga agagagggag agaaaatcat gctcaaaatt
     1021 tttctttgga atcggcaagt gatattcttc gatgattttc ttgtaataaa acaactcaac
     1081 gtcttttttc agatagtcaa aaaatgtttc gccagtcaaa aatcccgact gatgccattg
     1141 ctccaggttt tcttttgtta gtaaccgtgg ccatgccatt gctccgcctt gcttttgatt
     1201 tcttcctcga gggtaataag ggagccccct tcttgatcca ctgtatacaa tcggttcctt
     1261 gcctgatgga tgatagacca gttttccctt ttcgttctcg aagcgccctc ctcttgatac
     1321 tgtaaacaat ccgacataat caaaaaaact taagcctaga cctcttaaaa agacattttc
     1381 tttaggcggt atcttcgaaa gatctacatc tgcaggatta gaaggaagct gatacagtaa
     1441 aacatttttc tttgcgtagt ctacccacac tctttcttct tctgtctcat cattttccca
     1501 gtgaccgctt gctaaaacaa gctgatcgat agaatagctt tgctgatctg tctgtaaaag
     1561 ataaccatct tttcttttgc ttactttttt tactaaagaa taaacaaact gaatagaagc
     1621 tggaaattct tcttgcaact ttgagaaaaa ccagcgttga tagagtccgt ataaacatcg
     1681 agtagattgc tgatcttttt ccagacgatt gatttcttct ataattagtt gcttattttt
     1741 ggttaccgtc tgttcgatat aatctgtagc aggtccttta ctccattgaa acaagttggg
     1801 tccctgtgtt accaatcctc cactggacaa tgtttcatct gtaaaaagcg tcacttgttg
     1861 agaaacagaa ttcattaata actccggtgg ctgattcaac cgccaaactc gacctccagg
     1921 accattggga tcgaaaagaa aaagttcaag agaatgtctg tcctgattat tatggagaat
     1981 cctttctgct acacttaatc ctctaggtcc ggcaccaatg atccctattt tcatttgcat
     2041 gcctccattt cgataaattt tttcaataaa acagcatggt tctgatgttc attttttgca
     2101 ccataaacaa aagtaatgac tggatgttct ttcataagtt gactgagttt aaaaatagct
     2161 tgcttctttt cttcttcgaa aatcaattct tgctggtatt tctttgcaaa tgaagcaaat
     2221 cgttctggta catggtcaaa agttttgcgt gcttcattac taggtgcaat ttctttcaac
     2281 cacagatcta actgcgcttt ttcttttgaa attcctcgtg gccagaatct atccacgagt
     2341 attctatagc catcctcttt ttcagcatca aggtaaacac gttttaattt gatcatagtt
     2401 gtttccttct ttctaccttt tcgcttagct tatatatgta gataatagca caaaataaaa
     2461 aaaatgaacg gcacaactat ccattgcgcc gttcatgtga tcttggtcaa aatctaatga
     2521 ttgattcggt ttttaaagct gtcatacaat catctgtttt atactttttt acttttgatt
     2581 tttccagacc atccgtcata tccaccctta agtaaataaa tctcttgata acctgcttta
     2641 cgtaatttat ttgctgtacg gatgctcaaa cttttctttt gatcgtaaat atagacaggc
     2701 tggtcttttc gaatagatcc taatgaatct ttcaaaacag tgtaagggat gtttcttgca
     2761 cctaaaatat gcccagcatc aaacacatct ttttcgcgaa catcgattac ttgtgctttt
     2821 ctcattcctt ctcgaaattc ttcttctgtc aaagtcgttg ctgaacgttt ggccattact
     2881 cgtagataga cttcccaaag gatgattgca agcacaatca atagcaaaat gatattaatt
     2941 acccataaaa ccatgtaatg cctccgtttt tttaagcaaa tttcaaggct aatgaggcag
     3001 caccaatgac accagcttca ttaccaagct ctgctaattt gatttttgtt gattctctta
     3061 cttgcgggaa agtaaattct ttaaaatatt tctctacacg gctacgtaaa aattctcctg
     3121 ctgccgatac tcctccacct aacacgatac ttgacggatt taatgtattc cccaagtttc
     3181 cgcaagctaa acctaagtag aagcatacgc gatcaacaac catcaatgca aacggatcgt
     3241 cttcttgagc catttcaaag atatctttac ttgttacttc ttctccgttg tctagcattg
     3301 ctttcagttt tgaatcccct gcgtattctt ctgccaagaa acgacctagg cgtaccacac
     3361 ctgtagcgct agatacagtt tctaaacatc cgcgtttacc gcaagtacat tcgaaccctt
     3421 caggatccac agtgatatgt ccgatttctc ctccagctcc gctcacgccg tgaatcaaat
     3481 ttccgccagc aacgatacct ccgccaacac ctgttcctag agtaacgaat acaacgtctg
     3541 gatcgttttc tccagcacct ttccatcgtt cacctaaagc agcaacattc gcgtcattat
     3601 cgatagcgaa tttgattcct gttcctgcct cgatcagttt tttcacaaat tgaacttctg
     3661 tccagtttaa gttatacgca ccaataaccg ttccagcttc gctgtccacg cttcctgggg
     3721 tacccatgcc gattccgata aagtcttcgg ctttcatatt atataaattc aaacgatgat
     3781 tgattgattc aatgatttca ggaacgatat gcttaccatc atccaaaata ttggtatcga
     3841 tgctccattt ttgctgaatc tctccttctg gtgttaaaat tgcaaatttt gctgttgttc
     3901 cacctaaatc aatcccaata atcttcttgt ccattacaaa tttcctttct ttcggctttc
     3961 ttcaattcgg tgctctcttt ttaatatgcg ccacgctgtc atgtatgtat catgatcaat
     4021 caaacggcta tcatacagat tacgcaattc gatagtcatt aattcaatgt catagatgcg
     4081 tgcacccaca tagataaaga tgccaaattt tttgaacagc tgctgtacat catataagct
     4141 ctccatcttt aagcacttcc ttcatattat acaagtaaac catatttttt taaacccagt
     4201 aaaatacata ttacaaaaag aaatacaaaa actagtgtag aaagaatcct ttggtgtatc
     4261 gaatagcttg tctgttgttt cggtagaccc aaaacattcc ctaacactaa tccgccaatt
     4321 agcccaccga catgtcccca aacatctaca gaggtatcca gaagaccaaa aacaaatgtc
     4381 aacacaataa ataaaagaaa atgtctgact agctgctgga tcgctgggtt atgtttaaaa
     4441 tgaaacccta agataaacaa cgcaccgaac aaaccgaata tcgaagtact tgcaccacca
     4501 gacagaacat tcgcttcgtt aaaagcaaag cttgctgtat ttcccattat cccgctgaac
     4561 atataaatca agaaaaaacg ccaatggcca tagattgcct cgatttgctg ccccataaaa
     4621 tacagtatca gtgagttcat tacgaaatgc atcagtccaa aatggatgaa gattggtgtc
     4681 actaaacgcc accattcatt gaagtgaaca agataaggac catacattcc ggcataataa
     4741 gggatctcaa gtcgcggaaa taattccatc atgaggaaga ccgctgtctg tatccctaaa
     4801 aaagcatagg tccataacgg tcgtcttaac catgcacgca attttatttg ctgttgataa
     4861 ttcatgaaaa cctcccattt cattttagtc aataaacaat tgttggatct tctgatcaaa
     4921 gctctccggt tgccattgtt cattcaattg ttcacgaaaa acaaggctac atgttttttt
     4981 atgaaaatta tttaaatacc ggtcgtaata accaccgcca aaaccgattc gataaccggc
     5041 tctattgtag ataatacctg gtacaatgag gagatcgatc atctccggca aatagagctt
     5101 ctctgattgt ggctcaagaa caccaaaact cgaacgtaca agaacggact gttcatccat
     5161 ttcataaaac gccatctgac gtttcggcaa agttttaggt acaactattt tcttttgttc
     5221 tttttctgct cgttggataa ccggctgtgt atccagttca aattcattcg ataaagtcgt
     5281 ccctatcact tttgctgact tccagtactc gctggcgaat agctgtttat agattcgctg
     5341 ttctttttcc tttttcttgc cacttttggc cagatttttc agcttttcga ttcctttccg
     5401 gcgtaatctt actttcaatt ggtccaaaaa ctcacccttc cttctctcat aaaatggtat
     5461 aggaaaaaaa gaaaaatagc aatcgaactt tcaaataaat gcataaatag aataaaaaga
     5521 agacctagaa atgattggcc ttcctttttt aatgaatttc aactgttcag cgcgtgtttt
     5581 tgtctgataa aaagtgcata cagaaaatat ggcgcaccaa aaagggcaat caaaattcct
     5641 gtaggtactt ccccacttgg tagaatcact ctagcaacca tatctgcact aagcagaaaa
     5701 atggaaccga acaaaccgct cagaacaaag gtccagttat tttctttacg tacgactagc
     5761 ttcgcagcat gaggagctag tagtcctaca aaagataaac tacctgtcac cgctacacta
     5821 ccgcttgcca aagcaacagc taacaacaaa aggatttttt gacttttagc cacgttaagg
     5881 cctaatcctt gcgcgcgttc ttgaccaaat gatagcaata caatcctctg tgcataaagt
     5941 cctcctagcg gaattaagat aaccgcccat ggaagcaatg cccaaacata aggccagctc
     6001 gcaccccaga ttgatcccgc taaccatgca ttcacaaatc cataattttc cttagataat
     6061 tggatcgtgc atagcaatgt taatgcagaa aggccggcat taacggcaat accggacaat
     6121 aagattcgat tcatacccaa aaagccattt cgatcatagg ccgcaagata gacacagcaa
     6181 gcagctaaaa caccacctag aaaggctaca actagcaata gaaacggcag cgaatagtgc
     6241 gaaaagaaac ctaaataaag catcactaaa aatcctgctc cggcattaat gcccaatata
     6301 ctagaatctg ccaattcatt tcttgttact ccttgcaaga tataaccaga aacgccaaga
     6361 caagctcctg ctaataaact gatcagtaat cgaggaactc gaaattctct aacaatcagc
     6421 gccaaagaag atgattcttt ccctatgaac gtggcaaaaa gctgatccag gtaaataaaa
     6481 ggcgtaccaa tcattaaact acctatagac aggatgacaa gtacaaaaag caagaaatag
     6541 aacgtccaat atattttttt caccgttgtt ccctccttac ttgtatcagt aaaaacggaa
     6601 cgccaatcaa tgcaatgact aatccaaaag gtgtttcaaa agggggattg atcatcctcg
     6661 ctgtaaaatc cgccaacata accaatccac cgccaactaa aaaagagaca ggtagaacat
     6721 aacgatagtc cgttccaacc attccgcgaa caactggcgg aaccaacagc ccaataaatg
     6781 aaacaggtcc gactaacgaa acggcaatgg ctgctaacac tgcaactaga cagaaaacaa
     6841 tcaaccgcgt tcgtttagga tgacttccaa gagctaatgc atgtgtttca ctcaaatgaa
     6901 tcaaagatat tgcttttcct aatgaaaaag aaaccaaaac agcaagtata tataaaggaa
     6961 acaatactgc taattgtttc catgtgatat tggcagctcc gcctacaaac caaaaagcaa
     7021 gatcctgctg cagattaaag aattgggtaa gtgcttggct gatagcagta aaaaaagaac
     7081 taatagctac gccagctaaa atcagctgta aaggattcat gcccattttc gaaaactgca
     7141 aaaaaccaaa tacgagcaaa gttacagcaa aagaacctgc caatgacatt agaaacacag
     7201 aaaaatcaga agctttaggc caaaaagcaa aactaagggc cattgccaac gaagcacccg
     7261 cgttgattcc tagtaagcct gaatctgcta aaggatttct tgtcactcct tgcatcaatg
     7321 caccgctcaa tgcaaaacaa ctccctacaa gaaatgctcc cgccacacga ggcaaacgaa
     7381 tatttcgtat gatttggtga ctttgttttg atctatcaaa atcaaacaac gcctgccaaa
     7441 aatctgcttt ttccacagtc gctgcgccat ttgaaagagc aacaaaaaaa ataatcaaaa
     7501 gaacaaaaca gaccaaaagc aggagtctat ttttcttcat agtcctcata attgatcctc
     7561 ctgctctttt ggcaacaaat cataggttag aaaaactggt ttatttgttc ttggaagcgt
     7621 gacaatcgtt gcatcaatct caaatatttt tttcatacac tcttttgtga tcacttcttc
     7681 aggtgtacct tctgtgatca acttaccgtt tttcattgct aataaataat ctgaaaaacg
     7741 tgaagcatga ttaatgtcat gaatcgacat caagatcgtt gtttgtttca tttgattgat
     7801 ttcagtcaat aactgtagga tctctaattg gtgtgccgga tctaaaaatg ttgtcggttc
     7861 atccaagatc aagatatctg tttcctgagc aagcgccata gcgatccaga cacgctgggt
     7921 ttgtccacca gacagcgttg cgatatcccg catacgaaat ccagctagac ctgtagcatc
     7981 caacgcccac tcaattgctt catgatcttg cgtgtccatg ccactgaaca gtttttgata
     8041 agggtatcgt ccgtaagata ctacttcttc tactgtcata cctgtagggt gttcactagt
     8101 ttgtgctaat aatgcaattt tttttgcaag ttcttttgaa ctgtatgtag agatatcaga
     8161 gttattgaca atgatctttc ctgatttcac aggtagtata cgggtaaaac ttttcaataa
     8221 agtagatttt ccacttccat ttggcccaat caaactagtg attttccctt caggaatcgt
     8281 aacagacatg ttttcaatga tttttttgtt ttcgtagccg gctgcaatgt cagctgctct
     8341 taatctcgtc atttcaccca tcctttcgct gaaaatcatt ctcaattatg acgctgtttc
     8401 aacatttcgt caagcgctac tttcatatct atagccaaat gaagcggttg acagcgttcg
     8461 aaatatcagg aataataacc aatgagaaag attctcaatt atgttaggag gaaagaaatg
     8521 aaattaaaaa caaaagtatt cacattatct agcatattgc tgctggctgg agtactgagc
     8581 gcttgctccc cctcttcaga taaagaaaca gctacaactg ccaattcgac gacttcatcc
     8641 aatagtactc atacagtcac agatacactt ggacataaag tagagattcc aaacgcgccc
     8701 aaacggatca tcggcagtta cttagaagac tatctgattg cactagggga aaaacctatc
     8761 gcccagtgga ctgttggcag tggttccatt caagattatc tgcaagatga tttaaaggat
     8821 gtcccaacga tttcttatga ccttccttat gagaaagtgt taagctttga gcctgatttg
     8881 ctgttgattt cttcttccgc tactgtcgaa ggcgggaaat acgaacaata tagcaaaatc
     8941 gcgccaactt atgttgtaaa aaacggaaca gatgtcacat gggaagaaca actaaaagat
     9001 gtcggcaaag ctttggcaaa agaaaaagag gcagaaaaaa tcatttccga ttatcaaaaa
     9061 tccatcaaga aaactcgaga agaactagca gataaaatcg agaacaagac agctgcagtg
     9121 ttgtgggtga ccaacaactc agcctttatg gtcagcaaaa atcggtcaag tggaagaatc
     9181 gtatacgacg atctggaatt tggtgttcct gatctagtgg aagaggtctc caaaaaagcc
     9241 acttctgact ggtctgctgt ttcctctgaa aagctagctg aattagatgc tgactacatc
     9301 atccttgtga acagtgacaa agatgctgcc atgttcaatg aaccaagctg gaagaatcta
     9361 aaagcagtga cagacaatca tgtactgcaa ttcggacctg aatctagttg gctatataac
     9421 ggacctattg ccagtcagga aatggtcaat gatatcaaag aaaaactaaa ttgattttaa
     9481 ctaaaaaaag catcttcacg attagtcgtg agatgctttt tttcttatta acaaactaga
     9541 cgcatagatt ttattcttct tttgaagcaa ccctattttt tactctttcc catttttgaa
     9601 aacgatgggc atatagattt tcttgatccg ttatcccctc atactcctct ataagggcga
     9661 agtctgaaaa atcaatattc ccaatatacg tatcgccttc aaacgtgcta tcgatcaatg
     9721 ttcgccagat ccgaccagtg taaggaagaa gttcttgaaa aatccgacta ccacccgaaa
     9781 catagatatc ttctgagatc gtttgactat acgacaaaag ttcatcgatc gaatgcatta
     9841 cttccgcatt ttctttcgtg cgaaaatttg gttgcgttgt caacacgatg atatggcgat
     9901 aaggaagtga aagatccccc attccttcat aggtctttct tcccatcact aaaattcgat
     9961 ccattgtatg ttcgcgaaag aaacgcatat catttggcaa tcgccatggt aacaaaccat
    10021 ttttgccgat cagaccattt ttatcttgtg cccacatgga aataaacata ttatccgctc
    10081 cttatgattt tagatgtttt gagataaaaa aagtctcaat tctttgacca gcaaggaatt
    10141 gagaacttta aaaacattat tttgtttcac ggtgcaaagt aactttgcgt tcgcgtgggc
    10201 aatatttttg tttttccaaa cgatcaggat tgttacgttt gtttttgctt gttagatagt
    10261 tacgttcttt acaagaagta cactctaaag tgatgtttac gcgcatgttt ttccctccca
    10321 tttttacaag aatcctctaa gatgactctc agacttgatt atcttaccat gattttttct
    10381 agattgctag tgtttttcac gattttgtta agtaaaacta cttgacgata aatacaacgg
    10441 ttttacttct caaacaaaat ctttttcttt ttgtataaat acccttacac ttgtttatat
    10501 agattatgtt ttttaataaa gggggatact aatatgccat ggaacatgaa ggactttcct
    10561 gcatcaatga aaaacttaga taaattgaca agaaaaaaag caatcgacat cgcaaatgca
    10621 ttattagatg aagggtaccc tgataaccgt gcgatcccaa ttgccattga tcaagcaaaa
    10681 aaatggtatg aagatgctag tgaatcagaa aaacgaactt tcgaaaaaga aaaaaatcca
    10741 agcaagacgg atgaacacga tacgaatcct cgtgctggaa aactgcttga ttcagatgtg
    10801 atcgtagaat atgaggaaga acaatggatc gtgaaatcta aaggggcaaa aaaggcaagc
    10861 aaccattttg atacaaaaaa agaagcaatc gaaaaaggaa agcaagtagc acaaaacaaa
    10921 gaatcaactt tgttcattta taaaaaagat gggacaaaag aaaaagaaat cgattattga
    10981 acatatgtaa aggggttgtg acgaaaatcg tgtcacaacc cccttatgtt cgaaacaatg
    11041 gtgttcctct gccaactccg aaccataagt cataatatct ataaaacggg aagagctgtt
    11101 ttttgaaatt gtttcttatc actcagtgtt aaacggtagg agaaaacctc ttttttattg
    11161 atatttgatt ttcatcaaac agtcacatag tcgattgctt ctgcttgcgg aatcgtttca
    11221 tctacaaagt agactttata ccatgtcaag aaactgaaga ttgtccagat ttttctttgc
    11281 tcttctttac gtccttcgtg atggtcatct agcaacttca tgattttttc ttgatcaaaa
    11341 aattcttttg caaaatctgc ttcgaataat gctttgactt gttcgtagcc gtgttcttct
    11401 ttcaaccaag ctttgattgg cacagggaat cctaatttca cacgattcga ccattcttcc
    11461 ggaagatgtt tgttcgcagc ttgtctgaaa atatcttttg tgttgttttg attcaataaa
    11521 aacttacttg gtatttgttg agccaatttc atgacttcga tatccaataa aggtactcgg
    11581 acttccaaag aacttgccat agaaagttta tctgccttta acaagatatc ttttggcatc
    11641 cattgatgaa gatcgacata ttgcatctta ttgacttcat ctttgattcc ttctgttttc
    11701 tcgtaatgaa cagtcatgat ttcatccaca gatggtgctg tttgatattt tggtgttaac
    11761 acttctgatg cttctgattc ttcgaacact cgagcatgac cgataaaata ttcttttgca
    11821 ggagcaagtg attcatataa atggatccgt ccatggaagt ttttcatttt tccgatttta
    11881 cgtcctaaat tatagcgtgt tccttttggt aattttttca atccttgagc aatcacacgg
    11941 atgaattttg aattcgtatg gaatccatac gtttggtatc ccgcaaacag ttcatccgcc
    12001 ccttctccag attggactac ccgcacactt tgtgaagcaa gatttgctaa aaagtacaaa
    12061 ggtacgcaag aaggatttga atcaggttca tctaagtgat actggatcaa agggaatgct
    12121 ttgaacgaca tgtctccgtc gatcactgct gctgtattat ctaagtccaa tagttcggtt
    12181 agttttctgg cttcaatcgc ttcattatag gttttatcat caaacccgat tgagaaagaa
    12241 tgatctggtt tcaatacaga ggttacataa ctggaatcaa ccccgctgga taagaaagaa
    12301 ccaacttcta catcactgac cgtatgtgct tcaatagaag cttgaacggt ttcgtcgatt
    12361 ttttcgatcc attcttgtcg ggaatgcgtt tcttttgttt caaaatctgc atcccaatat
    12421 tgctgaatgt ctatttttcc atctttatac gtataataat ggccttcagg caaacgatag
    12481 acatctttga agaatgtttc gccgtttaat ggagaatatt ggaaagtcat ataaggtttc
    12541 aacgcttctt tgttcaattc tttgttaaaa tcaggatgag gcagaaaact tttgatttct
    12601 gaaccaaaca taaatgttcc gttcatttct gcataataat atggtttgat cccaaagtgg
    12661 tctcttgcac cgaataactc attcttttca ttatcccaaa tagcaaaagc aaacattccg
    12721 cggatctttt gcaataattc tgttccccat tcttcgtaac catgcaaaag gacttctgta
    12781 tctgcatgtg tttgaaaaac gtggccagct gcaatcaatt cttctctcaa aggctggtaa
    12841 ttatagattt cgccattaaa aataatgatc tttgtccgat cttcattgta aataggctga
    12901 gtaccgccct ctaagtcaat aatacttaat cttctgaatc ctaaagcaac atgtttatcg
    12961 atatattctc ctgaactatt aggtccacgg tgaacgattc gatccatcat tgtattaata
    13021 attgtttttt tgttgtcctt gtcgttaaca aaacctacaa taccgcacat ttaactttca
    13081 catcctaata taaaattact gtttatttca ttcatatgca tcttatcata gaataaatag
    13141 cggacgaata ttttctctct tacaatttcg tcacgtgaaa acaaaagatg aaagtttctg
    13201 tttttacaga aaataaagtc ccaatatcta atttaccatg ttttcttttt tgaggctacc
    13261 ttattctctt cgtgttaaaa aaatattagt ggatgctatt tccaaaaaaa ccagcaaaaa
    13321 aagacacgac ctaaagatcg tgtc
//
LOCUS       DACCXM010000072        13293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCXM010000072 DACCXM010000000
VERSION     DACCXM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.074"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            31..1929
                     /locus_tag="IVY61_001830"
     CDS             31..1929
                     /locus_tag="IVY61_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP7970942.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(2259..2978)
                     /locus_tag="IVY61_001831"
     CDS             complement(2259..2978)
                     /locus_tag="IVY61_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP7970943.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            3315..3647
                     /locus_tag="IVY61_001832"
     CDS             3315..3647
                     /locus_tag="IVY61_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970944.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3634..5637
                     /locus_tag="IVY61_001833"
     CDS             3634..5637
                     /locus_tag="IVY61_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP7970945.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5656..6126
                     /locus_tag="IVY61_001834"
     CDS             5656..6126
                     /locus_tag="IVY61_001834"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP7970946.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6147..6731
                     /locus_tag="IVY61_001835"
     CDS             6147..6731
                     /locus_tag="IVY61_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP7970947.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6746..7732
                     /locus_tag="IVY61_001836"
     CDS             6746..7732
                     /locus_tag="IVY61_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP7970948.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            7722..8033
                     /locus_tag="IVY61_001837"
     CDS             7722..8033
                     /locus_tag="IVY61_001837"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP7970949.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8055..9836
                     /locus_tag="IVY61_001838"
     CDS             8055..9836
                     /locus_tag="IVY61_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP7970950.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9829..11205
                     /locus_tag="IVY61_001839"
     CDS             9829..11205
                     /locus_tag="IVY61_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP7970951.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11212..11847
                     /locus_tag="IVY61_001840"
     CDS             11212..11847
                     /locus_tag="IVY61_001840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP7970952.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11877..13232
                     /locus_tag="IVY61_001841"
     CDS             11877..13232
                     /locus_tag="IVY61_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP7970953.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT         4311 a         2317 c         2974 g         3691 t
ORIGIN      
        1 ttgagtatac caatttttgg aggcgtttta atggaccgtt atttagattt actttcagaa
       61 aaattcccct caacggagga tgtcatcacg gaaatcatca atctcgaagc aattttgcag
      121 cttccaaaag gaacagagtt atttttgagt gatatacatg gcgaatttcc tgcttttgat
      181 catattctgc gtatcggttc aggaaatctt aaagaaaaga tcaaagacct tttttctgaa
      241 caaatgacgg aaacggatat cagccaattc actattttta cggcttatcc agaatatgca
      301 ttaacgactg attggtacaa agaacaagat aaatcgcagc tgatccatca tttgatcgat
      361 cttttacggt ttacgacagt caaatatacc cggtcaaaag ttcgaaaagg attgccgaaa
      421 gaatatagct atatcattga ggaactcttg tatatcgatg accgtatcaa tggaaaaaaa
      481 gactatgtaa aaaaaatcat tgaacaactt gttttgatga aggaagaaga aaaattttta
      541 acgaaacttg cacagacgat ccaaaaatct gtcatcgatc atttgcatat cgttggagat
      601 atttttgacc gaggaactca agcagccaaa gtgatggatc gaatcatgga tttttcttct
      661 gtagatatcc aatggggaaa tcatgatatt ttgtggattg gtgcgtattg cggctctgag
      721 gcatgtttac tgacactttt acggattgca gcacgctata attatttatt tgaattagag
      781 aaagaatatg ggttgaattt acgtccattg ttttcttttg ctcatcaaac ttatcagaag
      841 aatccagtat ttgcgcctaa aaacagtaaa aatctatccg aaagagagca agaagaatta
      901 gaaaaaatcc atcaggcact ttctatcctt caattcaaat cagaacatca gttattggat
      961 cgtcgacctg aatttaaaat ggacgatcgt aaattgctgg aaaaaatcga ctatgaagca
     1021 tcaacgattg atttagaagg tcagctctat gagttgaaag gaacttgttt tcaaacgatc
     1081 cagccagatg atccaaaaaa attcttggaa gaggaacaaa aagtgattga tagtttgatg
     1141 tattcattcc agcactcttt gagaatgcaa aaacatatga cgtttttgat cgaaaaaggg
     1201 ggaatgtatc tcacgaacaa tcagcatgtg ctgtttcatg gctgtatacc tgtagacgag
     1261 aagggacagc tcttagcatt tgaattagac caatcttatc gcggaaaaga actgctgggc
     1321 ttttttgaaa aacagataac tgaaagcgca aaagatctga ctgcaaaaga agatttagca
     1381 accgatttga tttggtatgc gtggagtggg ccattttcgc ctttattcgg caagagtaaa
     1441 atggcgactt ttgaacgtta ttttctcaca gagcctcata cgcacgtcga gaaaagcaat
     1501 ccttattatc acttgcgaga tgaagagtgg ttcagtgaaa aaattttagc agaattcgac
     1561 acaaaagaag aagggtcagc aatcattaat gggcacacgc cagtaaaagt aaaaaaaggg
     1621 gagtcaccga tcaaagcaaa tggaaaagta tttgtcattg acggaggttt atccaaagct
     1681 tatcaaaaaa cgacaggaat tgccggttat tcactattat gcaattctta tggtttccaa
     1741 atcgtgacac attatccgtt tttgcctatt gaacaacaat ttgcaaaaaa atccgaccaa
     1801 acaaatgtga aaaaagtaat cgaacagcaa ctaccacgca aactcaatcg tgatacaaca
     1861 aaaggtatgg aactgcagca gcagattacc cagctcaaaa ggctgttgga ctatcgaaag
     1921 gacgattgag aagaattcca gtgaacattg ttccatagac tggagactga gacaaaagtc
     1981 gtgtcgcagt ctctttttcc ggataaacgg tgttcttctg ccaactcctc ggtcacaagt
     2041 cacactattc aacaaacatg aaaagctgtt ttttgaaatt gctccttgtg actcagagtt
     2101 gaacggcagg ctcacgacct cttggtataa acggtgttca aaagccagtt tctcggtatt
     2161 aagccaaatt tatgcaaaaa tgtgaaaagc cattttcaca aaatttttct taatactcga
     2221 aactaaacag ctttttcaca accccctcag aaagtctatt aaaattcatt cacaaagaaa
     2281 tcgaataagc gctgttcaga tggatgtaca cggtgggtaa gttccggatg ccattgaaca
     2341 ccgaggattt tatgcatatc atctttgctt tgaaccgctt caatgagtcc atcgttagaa
     2401 aaagcgatgg cttttaataa aggtgaaaga tctttgactg cctgatgatg gtaagaattc
     2461 acaaaatatg gatcggggag aaggttgctt aacaaactgt ctgaaacaat cttgacttcg
     2521 tgagtcgtaa attgcggggc agtgggctgt tgttcatgtt ttactgacca ctttggatat
     2581 aatgaaagat cttgataaag tgtaccttcc aaaacgacat tcaatagttg gagtccccgg
     2641 cagactgcaa aaattggttt tctttgtttc aatgcttcca agatcagtgc ctgttcaaat
     2701 tggtctcgtt gaatatttgt ttccgttagc tttggatgag gatcttccat gtaaagttgt
     2761 ggactgacat cttgaccacc tgcaagaata agtttgtcga tttgtccaat cagttgagga
     2821 gctgaatctg gagacatgac tggcagaata agaggcaatc cttgagcatc taaaactgca
     2881 tccacgaatc cttgcggtgt atagctcact tgatttcctt ggaaagtatc tgttgctcga
     2941 atcagttgat ttccggcaat gccgatgatt ggtttcattt gtattccccc taaagtagac
     3001 ttattgtaac aaggatttaa aaaaaattaa actatgtaag acttgcccct atacttatct
     3061 gttcttgctg tgttactatc tgtaaagaga aatttcgtgt gaaattcatg acgatagttg
     3121 aaaatattgc ttaatttgtt ataataaaaa gtaagcagaa ataaaaaggt tgggcgtaag
     3181 cttcaaggat ctagttcttc aaggggtgag aaggacattt tattgaaggg gcctgcctat
     3241 actaagttat ctttagaggt acatactttc aatgaaaaaa gaaagcatgt acctcttttt
     3301 taaatcgaaa ggaagtgaag caagtgttag aagctttgga acaaatccga aaagcagaag
     3361 aagaaaatga aaagcgtgct caaacactgc ggcagcagct gaagatctat gcaacagaaa
     3421 aagagcagga attggaaacg atccaaacaa actatcgaca acaactaaaa cagttggtgg
     3481 aagaaaaaga acaatttgaa atacaagaat tagaaaaaga aaaaatgaaa ttgaaagcag
     3541 aagcagacaa ggtgcaagca actttgaaag atcaatatca aaagcatcag agtcaagcga
     3601 tcggtgcgat cattgaaagg gtgaaagaaa cttatggccg tcactaagat ggaaaaagtg
     3661 accctcattt cagataaaag aaatcaggag aggattttac aagcagtaca aggaatccaa
     3721 aacgttgaaa tccgggatct gtatcaaaat agaacaaaca accagtgggt ggagaagtat
     3781 tttccagata ccacgattat tgataaagaa gcaaaattaa gcgctttagg taatcggttg
     3841 aaagacatcc aagaagctat ccaattcata caacaccacg gcgatagccg tcaaaagaaa
     3901 ctccatttga aacgtcaaga actttcctta caagatttgg aagccaatta ttccgaagaa
     3961 aatttcataa ataaattgaa agaagtcctt gatttaaaag aaaagtggga acaactaact
     4021 gaagaacgtg atcgtttaga agaagaggaa gaatggcttc ttcattggca acagctggat
     4081 attgctccaa gtatgtatgc aagtaagacg accatttttg agatggggac tgtgagtgca
     4141 gcaaattttg aaagtttcaa ggaagaactg agtaagcttg atgacgttta tatggaagaa
     4201 atagattata cgccaaaaca agtttatctt tcgtatgttg ctttaaaaaa atcagttgct
     4261 gttatagaag aaattgctgg gaaatacggg ttcaataaag aaacgtatcc ttatgaacaa
     4321 aatcctaaag aaaaactgca agagaccaag cagcaattat ccattgttca cgagcagcaa
     4381 aagaagataa cgacagcgtt aggtcagtgc agtggctata tccaagaatt tgaatgggta
     4441 gaagaagtca cacttgccgt tgcagaacga gaaaaaataa aagaacggtt tatccatgcg
     4501 acttatctga tcgttttgca aggttgggta gatgcagaag aaaaacagaa tttgctgcat
     4561 gtgttaaacg aaaccgtatc agaagaagat atatacgtca gtttcgaaac accaaactca
     4621 gaagagattc agcaggaagt ccctactaaa ttgaaaaacc atccgcttgt cgaacctttt
     4681 gaattgctga ctgaaatgta cagtctgcca aaatatgaag aagtagatcc aacaccttgg
     4741 atgacaccat tttatcttgt cttcttcggg atgatggtcg cagatgttgg ttatggtttg
     4801 ctgatgctca ttgggacaat actagctcaa aaactacttg ttctaccacg cgggatgaaa
     4861 cgtttcgtaa aattctttga gattctctcg ataccatcga tcatctgggg attgatctac
     4921 agttcatttt tcggacagtc attaccaaaa gaactgtttg gcatccgctt gcctttccca
     4981 atcctatcta cgacggatga cgtcaatacg attttgattt tatccgtgat tttcggtctg
     5041 atacagattt tggttggatt atttgtggca gcaaaggaaa atatcaggag aaaagattac
     5101 ttgagtgctg tcagtgatgg atttgcctgg caagggatcc taattgggat cgttattgcg
     5161 ttggttggtg ccatgttgat aaaaaataca gcatttgttt accttggcgc tgggctatcg
     5221 atcattgctg ccttatgtat cttgatcgtt ccgattatcc aaacgccgtc taaagtcaaa
     5281 ggtgctgcca aaggtgcgta taatctttac ggactgaccg gctatatcgg tgatttagtc
     5341 agttatacac gtttgatggc attgggtatt tcaggcggaa gtattggagc ggcattcaat
     5401 atgctagttg ctttcatgcc gccagctgct cgattcagtg tagggatcct tttgatcatc
     5461 gctttacacg cactgaatat gtttttgaca ctgctaagtg cctatgtcca tggcgcacga
     5521 ttgcagtatg tcgaattttt tgggaagttt tatacaggag gcggacgtgc ctttgatccg
     5581 ctgaaaacgg cggaaaaata cgtcaacatc aatcacaaga aaaaaaacaa taaataattg
     5641 gaggattttt atataatgat ggattactta attagtcaaa atggtggaat ggtatttgcg
     5701 gtattagcga tggcaacagc gacgattttt tcagggatcg gttcagctaa aggtgttgga
     5761 atgacaggtg aggcagcagc agcattgaca acaagtcaac ccgaaaaatt cgggcaggct
     5821 ctgattctgc aattgcttcc aggtacgcaa ggtttgtatg gattcgttat tgcctttttg
     5881 atttttatca acttaggaaa tgacatgtca gttgtacaag gactgaactt tctaggtgct
     5941 tctctgccga ttgcttttac aggcttgttt tcaggtattg cccaaggaaa agtcgcagcg
     6001 gcaggtattc aaattttagc gaaaaaacct gaacatgcta caaaaggaat catttttgct
     6061 gcgatggttg aaacatacgc gattttaggt ttcgttattt ccttcctatt agtgttgaat
     6121 gcctaagcat aaaggaggaa aagcagatga acgccattga aaaaatcatc tctcagatga
     6181 acgaagcagc agagcaggaa agagcggcgt tagaacaaga agagcgaatg aagatcgatc
     6241 aaaactttga acaaaaacgt acgcaagttg aaacagaaca tcaaaaacaa aaagaaaaac
     6301 aaatcgaatt actcgaaaaa aaataccgtc agctaaggaa tcgccagcaa gtagaagtac
     6361 gacaagaaaa tttgaatgcg aagcaggaat ttttaagacg attgtttgca gatgcggtaa
     6421 ctgaaatgga aaattgggat gaatctgaac aaatccagtt tatcaaaaat gcattgtatt
     6481 cattaccttt gacaggaaaa gtagcattca ttgctggaga aaaatcagca gcctatcttt
     6541 ctcagacgtt gttggatgaa tggaacaatg aacttccctt tatgatggtt ttaagtgatg
     6601 aaactgttgc tgatcaagcg ggttttctga taaacgatca aggagttcag tataattttc
     6661 ttttcagcag ccttgtacaa gatatccaag gaacgatgag ttttgagatt gccaatcaat
     6721 tattcgaata aggagggatc agtgtatgga ataccacgaa ctaaatccct tgattcgcgg
     6781 aagagagctt gaacttttgt ccaatgagac atttgaacgg atgattcaaa caaattctgt
     6841 cgaagcactt ggtgaaatat tgaaatccac gatttacagt ccatatattt atgacggata
     6901 tgaatatgac ttcgaacaga atcttttaaa ggagcagagc cgtctatttc gctggttaaa
     6961 ggaatcagca ccggagcctg aaatcgtttg gatctatacg atgcgttata ctttccataa
     7021 tctgaaggtt ttgacaaaag ctgagatgac aaatcaaaat ctggaccatt tgtatatcga
     7081 cgatggattt tattcattag aaaccttgaa ggatgccatt catacgcaag tttcaaatga
     7141 acttcctgaa agcattatgg acagtatacg agaagtacgt gattattttg aagagtctac
     7201 cattcttcag ggaattgacg tgatttatga tcgacagttt ttgacagagc agcgacggct
     7261 tgccgataag ctaggatatc aagaattgct gacagaagtc attgctttta ttgacttgac
     7321 caatatcaca acgatggctc gaggaatatt gcagaaaaga acatatgggt tcatgacgac
     7381 ggtactttcc agctcgggaa gtatcgaaaa agaagtattt ttgaactttg tagataaaga
     7441 tatggaagat tacacgaatt ttctattaga gacagattat gctgattttc tgcgccctgt
     7501 catccgtgag ggaaatattg atctcgtaaa gcttgaacag ttgaaagatg attatctaag
     7561 ttcattgtat caagaagcac aaacccaggc tttcggacca ctgccactac tagccttttt
     7621 gaacgcaaaa gaggtagaaa gcaaaaacct tcgtttactg gttatcggca aaagaagcca
     7681 tttcgaatca gaaacaatca gaaagcgggt gagacaagtc tatgacgcat aagatcggtg
     7741 tagttggcga caaagattct gttttgccat tcaaattgtt tggctttgat gttcgttatg
     7801 gtacaaccaa gcaaaccgtt cgtcaggcaa ttgaagaaat ggcaaagaat gagtatggcg
     7861 tgatttatgt cacagagcaa tgtgcagcat tgagcccaga aacgatcgaa cgttacaagg
     7921 aacaaatgat acctgctatc gtattgattc ccaatcatca aggatcatta gggatagggc
     7981 tggaagaaat tcaaaaaaac gtagaaaaag cagtaggtca aaatatctta tagtatgaaa
     8041 aggagataga aattttgcaa attggaaaaa tcataaaagt ctccggacct cttgtaatgg
     8101 ctgagaacat gtctgatgcc agtatccaag acatgtgtct agtgggagat ctaggagtta
     8161 tcggagaaat tatcgagatg cgtggcgatg tggcgtctat tcaagtatat gaagagactt
     8221 ctggaattgg tccaggcgaa ccggttcgct ctactggtga agctttatct gttgaattag
     8281 gaccagggat catttcacaa atgtttgacg gaatccaacg gccattagat acctttatgg
     8341 aaatcacaca aagtaatttc ttagggcgcg gagttcaatt gcccgcattg gaccatgaga
     8401 aaaaatggtg gttcgaacca acagtagaag ccggagaaac ggtgagtgct ggagatgtta
     8461 tcgggatcgt agaagaaaca aaagttataa aacataaaat catggtgcca aatgggataa
     8521 aaggaacgat caaaacaatc gaaaaaggtt cattcactat tgatgaaaca gtatgtgtca
     8581 ttgaaaccga aaatggtgac aaaaatttga cgatgcttca aaaatggcca gtaagacgta
     8641 gtcggccaat caaagaaaaa ttaaacccgg atgcacccat gatcactgga cagcgagtga
     8701 ttgatacatt cttcccagtg acaaaaggag gagcagcagc tgtacctggt ccttttggtg
     8761 cagggaaaac agtcgttcag catcaaattg caaaatgggc cgatgtggac atggttgtct
     8821 atgtgggatg tggcgagcgt ggaaatgaga tgacggatgt tttaaatgaa ttccctgaat
     8881 tgattgaccc taatacagga gaatcgttga tggaacgtac ggtattgatt gctaatacat
     8941 caaacatgcc agtagcagct cgagaagctt ctatctatac gggaatcaca attgcggaat
     9001 atttccgtga catgggttat gacgttgcta tcatggcaga ctccacttca cgatgggcag
     9061 aagcacttcg tgagatgagc ggacgtttgg aagaaatgcc tggtgatgaa ggttatccag
     9121 cttatcttgg ttcacggctt gcggaatact atgaacgatc tggaagagtc attgcattag
     9181 gatcagaagg tcgcgaagga agtataaccg caatcagtgc tgtatccccg tctggagggg
     9241 atatttcgga accagtcaca caaaatacat tgcgggtcgt aaaagtattc tggggtcttg
     9301 attccagttt agcccaaaaa agacattttc cttcgatcga ttggatacaa agctattctc
     9361 tttattcaac agaagtaggg aagtatatgg atcatatcct tcaagctgac tgggcatcca
     9421 tggtcaccga gggcatgcgt attttgcagg aagaacagca gctgaatgaa atcgtgcgtt
     9481 tggtagggat tgattccctt tcagataatg accgtctgac attagaagtg gcaaaatcca
     9541 ttcgtgaaga ttatttgcag caaaatgctt ttgatgatgt cgatacattc acttcaagag
     9601 agaaacaatt caaaatgttg aagatgatct tgacctttgg tgaagaagga cgccgtgcgt
     9661 tgtcgttagg tgcttattta agtgagatca tggacggtac cgtatcgatt cgtgaaagga
     9721 tcggacgaag caaatatatc ccggaaacag aattagaaaa actagatcaa attattggtg
     9781 aaatcaagca aacgatgcaa acgatcgttt cagaaggagg catgagcaat gattaaagaa
     9841 tatcgtacga tcggtgaagt cgtcggtcca ttgatggcgg ttgaaaaagt tgctggtgtc
     9901 aaatatgaag aattaatcga agtccgtatg caaaatggcg agattcgtcg tggacaagta
     9961 ttggaagtcc aagaagataa agcaatggtc caaatattcg aaggaacaag tggtatcaat
    10021 ttacgtgact cttctgtacg atttttaggc catcctttag aactaggtgt atcggaagac
    10081 atgatcggtc gagtattcga tggtttggga cgaccgaaag ataacggacc agagatcctg
    10141 cctgaaaagc atttggatat taacggagaa gtaatcaatc cagtagctcg ggattatcca
    10201 gatgagttta tccaaacagg tatctctgca atcgatcatt tgaatacatt agttcgtggg
    10261 caaaaattac cagtattttc tggctctggt cttcctcata aagaattagc tgctcagatc
    10321 gcgcgacaag caacggtact cgattcatca gatgatttcg ccgtagtctt tgctgctatt
    10381 ggaatcactt ttgaagaagc tgaatttttc atggaagatt ttcgtcaaac gggcgcaatc
    10441 gaccgttctg tcatgtttat gaatttagca aacgaccctg caatcgaacg tatcgctact
    10501 cctcgtatgg ctttgactgc tgcagaatat cttgcttatg aaaagggaat gcatgttttg
    10561 gttatcatga cagacatgac caactatgca gaggcattgc gagagatttc tgcagcacgg
    10621 cgagaagttc caggaagaag aggatatcct gggtatcttt acacgaactt agctacttta
    10681 ttcgaacgag caggaagaat ccgcggacta aaaggatcgg ttactcaaat tccgattcta
    10741 acaatgccag aagacgataa aactcatcca atccctgact tgactggtta tattacagaa
    10801 ggacagatca tcttgactag agagctttac aagagtggga ttcagccacc aattgatgta
    10861 ctcccgtctc tttcgcggtt aaaagataag ggaactggac ccggaaagac aagagaagac
    10921 catgcagcta cgatgaatca gctttttgca gcctatgcac aaggaaaaca ggcaaaagaa
    10981 ttagccgtag ttttaggtga atctgcttta tctgatgtag acaagatcta tgcgaagttt
    11041 gctgagcggt ttgaaaaaga atatgtcaat caaggattct acacgaaccg ttcgattact
    11101 gaaacacttg atctagggtg ggaactgcta tcgatgttgc caagaaccga attaaaacga
    11161 atcaaagacg acatgctgga caaatatctg ccagaaggga agtgagttct gatggcacga
    11221 ttgaatgtga atccgacccg catggaattg actcgattga aaaaacagtt gacaactgcc
    11281 acaagagggc ataaattatt aaaagataag caagatgaac tgatgcgtca attcatcctg
    11341 ttgattcgaa agaacaacca gctgcgtcaa gaaatggaag agtctatgac agaagcaatg
    11401 tctgattttg tattggcaaa tgcttcgatc acggaacctt tcatcgaaga attatttgtt
    11461 ttgccggcgg aaaacgttga tttgtctatc gtagacaaaa atatcatgag tgtaaaagta
    11521 cctgtgatga attttgaata tgatgaacag ctgacagaca cacctttgga atacgggtat
    11581 ttgaattcca gtgccgaact ggatcggtca atcgatcggt ttactaccct gcttccaaaa
    11641 cttttggaac tgacagaaat cgaaaaaact tgtcaactga tggcgagtga aatcgaaaag
    11701 acacgtcggc gagtcaatgc attagaatat atgacgatcc ctcaattaga agaaacgatc
    11761 tattatattc gtatgaagct ggaagaaaac gagcgcgcag aagtaacaag gttgatcaaa
    11821 gtaaaaaata tgggtaccac tgactaagtg gtatcttttt ttaaaagagg aaaacgatga
    11881 ttaaacaaag caaacaaata aagaaacgtt tgccggctgt tcagcttctc gcattaggat
    11941 tttttctcct gattttagtt ggcggcggcc tacttaccct accatttttt agtaatagtg
    12001 ggcatgggac aaattttatc gatgcgcttt tcactgcaac ttctgctgtc tgtgtaacgg
    12061 gattgacaac attgaataca gcagagcatt ggaacagtgc tggtcaattt ttgatcatgc
    12121 ttttgattga gatcggcggt cttggtttta tgatggttcc gattctatgc tttgcattga
    12181 taaagaagaa agtcagtctc agcacacgta ttatattaaa agaagcgctg aatctagaag
    12241 aagtatcagg tgtgatgaag ctcatgcttt acatcctaaa attggccttt gtgatccaat
    12301 tgattggtac ggttgcttta tcttttgtat ttgtgcctga atttggctgg ttgaaaggta
    12361 tctggtacag tttgttccat gcaatctcta gtttttgtaa tgcagggttt gatttgttag
    12421 gagacagttt agccaatcac cagcaaaatg tttatttgat catggtggta tctgctttga
    12481 tcatttctgg tgggttaggc ttcatcgtat ggcgtgattt actggaatat catcagaaaa
    12541 agaagatgac gattcattca aaaatcgcat taaccgttac aggtaccctt ttattaggcg
    12601 gattcgttgt gttctttttc acagagcgca atggggcaac gctagtggaa gggaatatgt
    12661 ttcaacgatt ggcaaacacc ttttttatga gtgtgacacc tagaacggct ggatattatt
    12721 cagttgatta cctccatatg agccatgcag gtctgatttt aacgatgttt ttgatgtaca
    12781 tcggcggaac atctgggtcg acggccggcg gattgaaaac aactacgtta ggtgtgttgc
    12841 tgatccagat gcgttcgatg ttgaaaggac gtacgagagc ggaagcattt ggccgtacca
    12901 tccgacaagg cgcagtgttg cgggcactta ctttattttt tgttacgttg acgttgtgtg
    12961 tagtcgccat catgatttta tcggtgactg aaacgatacc taaaacttct ggcatcgaat
    13021 atattgcttt tgaggtcttc tctgcgtttg gtacagtagg tcttacgatg ggattgacgc
    13081 cagacttgac tgcaataggg aaattgatca ttatagcgct tatgtacatt ggacgagttg
    13141 gtgtattgac tgtggtcttc tctctgatga caaaagcaca aagaacacaa tcaaaataca
    13201 aatatccaga agaaagtgtc atgattggat aatttcatct tctgacagaa acctagtgat
    13261 ttagtaaaat aagaaaataa aataattttt ctt
//
LOCUS       DACCXM010000073        13210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCXM010000073 DACCXM010000000
VERSION     DACCXM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.075"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(334..894)
                     /locus_tag="IVY61_001842"
     CDS             complement(334..894)
                     /locus_tag="IVY61_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7970954.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            1036..1650
                     /locus_tag="IVY61_001843"
     CDS             1036..1650
                     /locus_tag="IVY61_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7970955.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1839..3719
                     /locus_tag="IVY61_001844"
     CDS             1839..3719
                     /locus_tag="IVY61_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7970956.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3798..4025
                     /locus_tag="IVY61_001845"
     CDS             3798..4025
                     /locus_tag="IVY61_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP7970957.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            4030..4572
                     /locus_tag="IVY61_001846"
     CDS             4030..4572
                     /locus_tag="IVY61_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7970958.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4799..5515
                     /locus_tag="IVY61_001847"
     CDS             4799..5515
                     /locus_tag="IVY61_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7970959.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(5575..7593)
                     /locus_tag="IVY61_001848"
     CDS             complement(5575..7593)
                     /locus_tag="IVY61_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP7970960.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7606..8604)
                     /locus_tag="IVY61_001849"
     CDS             complement(7606..8604)
                     /locus_tag="IVY61_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970961.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8802..10043
                     /gene="sstT"
                     /locus_tag="IVY61_001850"
     CDS             8802..10043
                     /gene="sstT"
                     /locus_tag="IVY61_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP7970962.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            10190..10825
                     /locus_tag="IVY61_001851"
     CDS             10190..10825
                     /locus_tag="IVY61_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7970963.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            11016..11756
                     /gene="truA"
                     /locus_tag="IVY61_001852"
     CDS             11016..11756
                     /gene="truA"
                     /locus_tag="IVY61_001852"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7970964.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11901..>13210)
                     /locus_tag="IVY61_001853"
     CDS             complement(11901..>13210)
                     /locus_tag="IVY61_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970965.1"
                     /translation="YFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALT
                     FLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQ
                     KIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNN
                     NYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVK
                     SLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHA
                     NKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSN
                     KLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVI
                     TYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4398 a         2096 c         2584 g         4132 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgatc
      721 atatagatca tagatatcct tataatgtgt ataaaaagtc gaacgatgta catctgccgt
      781 ttctgttagt tcgcgtatag tgattgcttg aatcttttta tttactaata atgtggatag
      841 tgtttcagcc aaagcttttt ctgttttttt gacgcgtcta tctgtctttt tcatttacct
      901 atctccttat ccgacatatc aaagcaaagc tgtcgcttaa tcgacaaact tatagatgat
      961 tgattataga tagtagacac tgtgtctatt ataatgtaag tataacacaa tcattgaagt
     1021 tgaggaggcg aaatgatgaa actaactttt aaacccttag aaaagaaaga ttacaaaaaa
     1081 gctatctatt ttgctgttga aggaatgaac tttgagagat atatggaaaa tgaacttctg
     1141 ctgaatcttt acggaagata ttttttgtat atggagttga atcgagcaac tcaaattctt
     1201 gctttgtatg cagaggacga gttggcaggt gttttgctgg cggagataaa aggagaaaaa
     1261 aagaaacatc gttcttttgg acagcaaata tatgttaaga tattcgattt tttacaaaat
     1321 atatttgcta aagaaagtaa aggagcttac gagacagcaa atgaacaaat gcttgcagca
     1381 tatttaaaga agcattcgcc agatggagag atcatttttt tagctgcaaa tcctgatttg
     1441 aaaacaaaag gcatcggtac caagttgcta acagaatttg aaaaaagaga gaaaggaaaa
     1501 gaaatattcc tttttacaga tagtggctgt acgtatcaat tctatgaacg acgtggattt
     1561 gaaagactaa aggaaaaaat tagcaagata aaaatcttag ataaagaaat agatctccga
     1621 tgtatgctgt acaggaaaat cattcaatga aaatttcgat agaaacgaca atagttggca
     1681 agatcacttt tagtgagtct tcttgctaac tattttttgt agtgtattct accaaaagaa
     1741 aaccgccttt ttgactaaac ttgatagtgg tcaatttttt atgatggata agtatctata
     1801 cttagtctcg taagaatgaa aggaaaagag ggagaaagat gaaattacaa aagtggatcc
     1861 aagagcatcg aaatcatatc accgccttat cagctgtgtt gattattttt ggttttatcg
     1921 gaaaatttac tggttttgtc ttgttctata acattgctct agccatagct tctattattg
     1981 cggtcatacc tattatgatc catgcgtatc aggcagtgaa agtcaaagtt atctcaattg
     2041 atttactcgt tactattgct gtgcttggtg cctttatgat cggagaatac aaggagtcag
     2101 ctatcgttac attcttgttt ttgtttggta gctatctgga acaaaaaaca ttgaccaaaa
     2161 cgcgggagtc aatcaaggct ttgaccgaga tggccccatc tactgctagt gttgtccaag
     2221 aagatggaac gactgaagaa gtggaaatcg atgacgtgga agaaggcgat atcgtcttag
     2281 taaaaactgg tggagctatt cctgtggacg gacgagtcat tgacgggaaa gggtatgcgg
     2341 atgaagccgc agtgactggt gaatcacgac aagtcgtcaa aatcagtgga gatgaagtct
     2401 tctctggaac gattttagca gatggctacg tgacgattga agcaactaag gtcggagatg
     2461 atacgacctt tgccaaaatt atcgaattag tagaagaagc acaagatacc aaatcccatg
     2521 cggaaaaatt cattgatcga ttttctcagt attatactcc aaccgtttta cttattgcgt
     2581 tggttgttgg tctgattagt cgtgattttc gtttagcaat cactgttttg gttcttggtt
     2641 gtcctggcgc tttagtcatt ggtgcgcctg tttcgaatgt agcaggaatc ggtaatggag
     2701 cgaaacgggg catcttagtc aaaggcggtg aagcaatgga tactttttcc aaaatcgata
     2761 cttttgtctt tgataaaact ggtacattga ccaaaggaac tactgctgta tccactatca
     2821 agaactatac agaggatgaa ggggacagct tagctttagt ggctcgatta gaaacgttat
     2881 ccgatcatcc tctggggcgc gccgtgatcc attatgctca ggagaaagac attgattttc
     2941 aacaattagc agtcaaagat aatcagacaa tcaagggaca aggaatcaca gctgagattg
     3001 atggtcatac agtttgtgcg ggcaacacca aattgattgc cgctcacaaa ctttctctca
     3061 caccaaagca agtacaagat ttacatcaat tacaacaaac aggcagctcg gtcattattg
     3121 tagcaatcga caaaaaaatc actcaaatca taggtgtgtc agatgtgatt cgaccagaag
     3181 tcgctgaaca attagctaag ttgaaacaag caggagcaaa acagttagtg atgctgacag
     3241 gtgataatca gatgactgct gattatgtag ctgatatgtt aggcattgat gaagtccatg
     3301 ctgaattatt accagacgaa aaggttcaat ttgtgaaaaa ataccaagaa caaggattgc
     3361 gcgttgcttt tattggagat ggaatcaatg attctccctc tttggctgcg gctgatatcg
     3421 ggattgctat gggatcagga acggatgttg ctatcgaaac gagtgacgtt gtcttgatgc
     3481 aatctaattt tgaatcgttg gttcatgcct accgcttggc taagaaaact gttttaaata
     3541 cccgtgaaaa tgtagtgatt gccattggtg tcgtcttatt tttactcaac ggcttatttg
     3601 ccgggtttat ttacatggct agtgggatgt tcgttcacga agctagtatc ttgatcgtta
     3661 tctttaacgc gatgcgttta ttgtattttg gacacgaaag caaagaagaa aaatcgtaaa
     3721 acttgataag aatcaatttt tttatggaag ctgtaatcta gaataagact atcaagaaaa
     3781 aggaaggaag aactacgatg aaaaaaatta ttatgcaatt agatacacta gcttgtccaa
     3841 gctgtatgca aaaaatcgaa catgcggttt ctcaacagcc tggggtaagc aatacgaaag
     3901 ttttgtttaa tgcgagcaaa atcaaagcaa actttgatcc agatgaagtc caccctgaac
     3961 aattagctgc agttgtggaa aaattaggtt atgaagtcca atcagcaaaa gtaaaagagg
     4021 cgtaaatcaa tgaatatagt ggaagaaaaa tttgttcaag aaacaaaaca agcagatatc
     4081 gaccatcata aaccaactgc tggcgccatg gtggggcatg tattagcaaa ttttaaatta
     4141 gaagaaatga aattgatcca agcacaattg tatgttttgg gtacatcttc gcaagaatta
     4201 agtacccttt tttcagaata tgctgatcaa gaagcgagat ggttcaataa aatctctcat
     4261 gagttagtga ttgaaaatga aatcgttccg tctactttag aagaagtcat gcgttatgga
     4321 aaaatttacg aacatggtca aaacaaatat ttaccagcct caacaatgtt ggaaaacttt
     4381 gcaaaagatt ttgatttcca aaatgtcttt atcacacggg caatcaaatt agctgaaaaa
     4441 gaagaaaaat atggcttgca acaattgctt gttgaacttt taagtttcaa taaagaaatc
     4501 atctaccaag tacaaagact ccaaggaaaa actgtccgac aagatttgga tgaggaagac
     4561 gatgatgact aaaagtctat gtttcttcaa atacaaatat aaagcgtgag gtaaattgga
     4621 aaatcacgaa gattaaaata aaagaagcac ccgctgatgt attacagaca acatcagcgg
     4681 gtgcttctca gtttttagaa gatttgaaag attgatgaga aaatacgaaa aatcaataaa
     4741 atcacattgc ttatttgata gaataaagaa gtgggagtaa tcgaaaggag ttttttaatt
     4801 gaaaaaaagg attttagtaa caattggtct agcttttttg ttagctggtt gtaccaacgg
     4861 gaatgaatcg aaaaaagaat caacaaaaga aacagcgaaa gcaacagttg caactactac
     4921 aacaactagc acagagagca agtcaactgc agcaagtaca gaaacagcag atacctctac
     4981 ttcgtcagag tcttccaaaa cgactgaaac aactcaaaca tctacttcaa gttcttctgt
     5041 agaggcttca tcttcctctt cagaaagcag cgaacaagtt gaacaaacac tgtggaatgc
     5101 agacaaatcc gcacaattag aggcatttat gaccaaatgg ggccaaacaa tgggacaaca
     5161 atacaaaagt tatacgaatc aaatgagtgt ggacttatat ggtcttaaag tccctcaagc
     5221 tattttgaac ggggagtgga agatggctat tggtggagtg cctgtttctg cagaatggtc
     5281 agaaagtggg acaggtcaag cagactatca aatcacagcc gtctattccg atgcagagac
     5341 agaaccatat ctgaaaaagc acgtctatct attcgggttt cagcaaaatc agccaaaagt
     5401 attggtgaca caacaaaatc aggggaatcc cgataattat ctttacttca acgagacagc
     5461 gaataatgaa ttgaaaaatg gctttaatca aatcgtttat ggacaagcaa attaatgaga
     5521 taaacaaggg gttgtgacat gtcttttatc acaacccctt gctcagttta tttgttagtc
     5581 ttttattttt tgtgtcgccc atgttgcact gtactggtta cctgcaacaa gatcatattg
     5641 tatcagacga taatcttcta gaatttcttt ttgtttctca gttaaatttt tttcttggat
     5701 aatttgacta tcctctgtaa caaagacctg ttttccatta aaacgattag acaagctttc
     5761 tgtaggatca attgttaatg caggtagatg attagatact tctgtcaaca acgcatagta
     5821 aggagttact ttgatattcg catgctcaag agccatagca ccaaagttat taggagcaac
     5881 aacttcttta tttaacgtag ctgcttgttc acgactatac ttgttgttat aaatgaaata
     5941 atctgtctgg tgctgttcta atccatattt tttcgaatca ttcccactat agattgcggg
     6001 taaatggtca ccataaaaaa caaaggtaat cggtttatcg attttgtcga tttcttggat
     6061 gaattttttt actgctgtat cggtatagtg aaggccttgc atataagttt gtaattcttt
     6121 tcttctatta ggaatgacag cagttccttc agcagtgtag ttcaattcac tatagtaatc
     6181 atcataaggc atatggtttt gcatagtgga taattggatg aattgtgacg tttccgtatt
     6241 gctttgtaaa gctttcaagg tttctttgta tgctgaatca tccgatatgc gtgggctttg
     6301 ttctatttta tccgtataag ttaatttatc cggactgtct aaataataga acctgtcaaa
     6361 accaaattta tcaaacactt gttttctgcg ataaagagca gctgtaaaag gatggatggc
     6421 tattttttca tcgaatagat cggtaatatt tggacttatt ttctgacgct ccactagctg
     6481 ggtataaggt gtaactaacg aagcagacag gttactgata ctcaagcctg tcagtccttc
     6541 ccattccatg ttagctgttc cgccaccata accgacggat aacatcaatc ctcctgttcc
     6601 ttctgttata atgcttttga tgtaaggaat agggtctgta ggtactgtca gattagggac
     6661 acgtgcagga tcactgaagc tttcacttaa attgagaacg atcgtctgat tgtttaacca
     6721 gtcacttctt gtttggttga tttcatctgc ctttgtgttg tactttttca ttattccatt
     6781 aattttttct ttcgaatagc cagcaggttc ttccatgatt ttaatgtcga tattgtttag
     6841 gaattgaatg actggtccgt tatcttttac ggcgccttgc tggttaaaga aagtcttgtt
     6901 tactttaaat aaattaaaca gcaagaaaga aggcgaattt ttgtgattaa tgaaaaagac
     6961 acccgaaaaa catcctaata atacagcgat actgataaat cgacgtttcc aatgaatctt
     7021 caaggaatac tgtttttgta gtctacgttg tacaataata ctagtaatca ataataggag
     7081 aaggatcaat ccgcccacga ttaataagac tgggctaacc atcgataata gttctgacaa
     7141 tccggtcagc atttttaaat ctgaaggaaa gacaggctct tcccgcattt ccattttgat
     7201 tactgtcgaa atagtgagca gtaagttaat gatcaaggta gcagtcaagg cgtaccaaaa
     7261 acgattggaa atcaacaata gtaagaagaa aaatcccata ataatgatga ttgtcaggat
     7321 catgggtgct tgtcgctgag taaatatttt taaggcaacc tggatagttt ctttccaacc
     7381 ttcttttggt tctaaaacga aaatctgcac gaaggtaaag cagtaaaaaa atgctgcaac
     7441 taaaaaataa cttttatgaa caaaaagcca atgaattctt tttttccaac gctgagttaa
     7501 ttgttcaatc gaatcaaaag cgaagtattc gatgaaacga tgaatgaaac ggattttttg
     7561 tatataaaat aaaaagataa ctaggaggga cactgctaat aaatatcaaa taaaaaactg
     7621 cccgatattt atcagccatg ccttccctga gtttggaaat tcacgtttca taaatgtaga
     7681 tacgcgataa gaaactaaag ggaaattaca tataacttga gtgacaatca aaaatacagc
     7741 aagaaaattt cctcttcctt ttatacctaa tttatcagat agcatttcta aaaataaaaa
     7801 cgaagagaaa gtataataca ttgcgaagag agaatatggt ttagaaaacc tcagcgctgt
     7861 atctgtattt cctctgatga tgagtgaaat ttgtgtcatc aaaataatca aaccgaataa
     7921 aacaatcaag ctgataaata cttgaggaag cttatagcgg accttttctt tccagttcca
     7981 ttctttcaaa ccataaccca ttaaaaataa ataaaaaatc cataaaatac tctgtccatt
     8041 ctgtaatgca aacgggtctt tattaaatag agtagagcag ccaactaatc caattgttaa
     8101 aaataatagt agatatttaa ttgttgtttt cgataattta tttaaaaatc caactgtata
     8161 aggaatacta caaaaaagca aagtacaaga aacagcataa gtaaagtaat tctgactgat
     8221 aggtgttagg ataagccaat aatcacgaat attcaagctg ccagcttgta cgtaaaaaaa
     8281 gatattgcaa actagtgtgg ctattaggaa tataaaggca aaacgactta aataatgtaa
     8341 aacttttaaa cgggcgattt tttgatattg gtaacccatg taaagtggga tcaaatttgc
     8401 accaataaca acggaagttt gtaaaaatgt atagatgtag aatcgaccag ggtttgttgc
     8461 atatgtagga atagtgactg ctgaaatcaa tatacgctgt aaaaccatca ttatacaagc
     8521 gacaaagatt gtcaggaagc cataactctc cttctttatt tttttcaaga ataaacacct
     8581 cacaaaaaaa ttggacaaaa acataagaaa acgtttcctg ataagttgcc cattaataac
     8641 atcctaactt agaatatctc aaaaaacaac gtgtagacag aaaaagtaca aaattagcaa
     8701 gaaatggttg tttgtattgt tagaattttt agaaaattat acctttagtt tctttgcata
     8761 aaatgataca atgattgtta aaattgtgaa agggtggtca tttgttagag aagtttttgg
     8821 atatgtcgtt agttcaacgt attgttgttg gtattttagt cggaatattg tttggtatct
     8881 ttttccccac atggacattt atttcaatat tgggaacgtt gtttgtcagt tgcttgaagg
     8941 caatcgcacc tattctggtt tttttcttga cgatggcgtc aattgccaag cataaaattg
     9001 ggaataagac attcgtgaag cctattttga tcttatattt agtgggaaca ttgctttctg
     9061 cacttgtagc agttattgta agttcaattt ttactgttcc tattacttta caagaaacag
     9121 tatctgaaaa agcaccgcaa agtttggaaa gtgttttatc tactatgttg acgaatgtca
     9181 cacaaaatcc tgttcaatct cttattgatg cgaattacct tggcgtctta ttctgggctg
     9241 tattgttggg actagctttt cgagcacgtt cagaatcgac aaaagagttg gtggatcaga
     9301 tatctatagc gttgtcacaa gtcgtacaag tgattattgc atttgctccg atagggattt
     9361 taggattggt ttatcaatca attgctacga ctggaatcgc tggattggca gagtatctgc
     9421 agcttttact tgttttgatc ggtacgatgc tgtttgtcgc attagttgtg tacccattcc
     9481 tcacgttttt gtttattaaa gaaaatccct atccattgat tttcttttgt ttgaaagaaa
     9541 gcgcacttcc cgctttcttt acccgaagtt ctgccgcaaa tatcccgata aacatgatgt
     9601 tggctgaaag attgaaattg acaaaagagt cttattccat atcgatcccg ttaggtgcta
     9661 cgatcaatat gggcggtgcc gcgattacca tttccttgat gacgttgact gctgtacatt
     9721 ctttagggat tgaagcacct tttgttttga aagtcctttt atgtattttg tctgctttag
     9781 ctgcttgtgg agcttcaggt gttgcgggcg gttcactgtt attgattcca ttagcatgta
     9841 gtttgtttgg tatctctaat gatgtcgcga tgcaaatcgt cggtattgga tttattatcg
     9901 gtgttatcca ggattcaatg gaaactgcct taaattcgtc aagtgatctg ttgtttactg
     9961 caataggaga gctaggcgcg cgtaaaagaa gtggggaaaa aatcaaattg aaagattgtt
    10021 tagatggatt aagtgaacgt tgaacgttag atatttagta tttttaaaat gattcgagtt
    10081 tttaatgagt tatatagatg ttcgtgtaaa tagaggtttc tgttaaatca agataaagtc
    10141 gtttataata acaattaaaa gcataggtaa ttgttattgg aggcgtatca tggatttttt
    10201 acgtaaattt ggttttttct ttttattatt aggcgtgctg agtgattttt tgactccgta
    10261 cgtattagga ttgtattatc ctgaattaga tcaaatgaag agcgtgatca gtgtatttgg
    10321 agaggtttcc agtcctgtaa gaaaagcgtt tcttatttgg tctgtggttt cagggatctt
    10381 tttcatcctg tcattgcctg cattgtatgc tgcatttgtt agtacatctc gtgtgttagc
    10441 taccctagtt acagcagcca tcgcgttcta tggtataggt gattgtatct ttacaggttt
    10501 gttcagtatc aatacccatg aatcttcgtg gaatgtctcg acatgggtcc ataatatcgg
    10561 atcaggttta ggttacagtg gttttttatt gtttccagtt cttcttttct tgatctatcg
    10621 aaaaaatggg caaatggacg taagccactt ataccttgga ctgtctgtga tcagtttgct
    10681 ttttgcggcg atttatggac tggcaagaat tccttctata aattcttggc cattactgaa
    10741 taaaataggt ttttgccaaa gaatcagttt tttctttaat tatgtgccca ttgcctggtt
    10801 gagtatcttg catctcaaac agtaaaactt gagccgtcaa attgaacagt cagcggatac
    10861 ttcatccggt ggctgttttt tgtttttctg aaatatatat ttccacagtt ttcttgtata
    10921 gcttctgtct tagagaagca aggagctata ttttgtgcta caatcttaaa aaggtagaga
    10981 gaagaccaaa tggaagacag aggagccact aaaatatgag aaacatcaag ttgacaatcg
    11041 aatatgacgg aaaaagatat tcaggatggc aaagacttgg agatgacgat aaaaccattc
    11101 aaggaaaaat cgaaaaagta ttgcatcaga tgacaaatga agagatagaa attattggat
    11161 caggaagaac agatgctggc acccatgcgc gaggacaagt agcaaatttt aaaacaaaca
    11221 cagaaattgg actggcagaa atgatcgact ttatgaatcg ttaccttccg cgggatattg
    11281 tcatcaaaag aatcgaagaa atgcctgagc gcttccattc aagatataat gcggtgggca
    11341 aaaaatacag ctattatgtt tggaacaacg tgattccttc agcatttgag cgaaaccaca
    11401 gcttttattt ccctcaagag ttggatatgg acaagctgaa tgcagcttgc gaaaaattag
    11461 tgggtacaca tgatttcatt ggtttttctg cattgaaaaa atcaaaaaaa tccacagtgc
    11521 gtacgattga tgaattatct atcgaacgaa agggagaaat gcttcatttt acttttgtcg
    11581 gagacggatt cttatataaa atggtgcgga ttatcatggg gacattacta gaaatcggca
    11641 caggtgccat gccgatagaa acaatcgatg aaatatttga aagcaaagtg cggcaacatg
    11701 caggagaaac cgtccctgca caaggtctgt ttttggatga agtttattat cgataaaaat
    11761 actttgataa tggatgaaac gcatgtttca cagtataaat atcgatgtta aataatgtat
    11821 gaagcaaatt aaagaaatat aaaaaaagac tttttagcct gtttatgtgt acagctaaaa
    11881 agtctttctt aaataaaaat ctagtgagaa tgttgtactt ctcttctcgg ttgtttttgt
    11941 ttagaaaaag taaatcgata aagtaaaaat aaaatcaaag taacgataga taaatatctt
    12001 gaagcaattg ctagttttgt gtactgataa gtaataacga ctttatttat acctggagga
    12061 atagttaatt cagttgtgcc aaacttagat aattttgttt gtactagttt gccattaata
    12121 gaagccactt gccctttata acgataaata ggtgtcgtca agacagtatt tttattattc
    12181 gaattatcta attcaattgt aagtttgtta ctcataaaat gagttgttgg atttatttcc
    12241 tggttaccta gtaaatattg atcattatta atcatatcag gatgatagat tgactctttg
    12301 tttgcataat cagtatgatt gtaattttca gctatagcaa cagcgtcttc aggtgctata
    12361 agtgttaatg aatctttgtt agcatggata gaattgctta ctcctgagag atgcaatatt
    12421 agtgcaagaa acgtaaagac cattattttc catgaattat ttttagtaga aaaaatataa
    12481 tagatcacta tactcatcgt ataggctata aataaagaag aaaaaccatt taaccgccag
    12541 acaaattgta gactcttaac tggtgtatgg ttaaaaagtt gccaaggaaa taaattggtt
    12601 gaacaaaaaa gaacaaatac accaagaaag aaaataaata aatcatcagc agttaatttt
    12661 tttataaaaa taaaagcacc aatcaatccg agaaataaga ataatccaat agtatagttg
    12721 ttatagttat tatttaaaat atgtgtcagc atatcactag gagccatacc acttaattct
    12781 tttcccagtg ggactttcaa ttcttgtgct ctagattgtt gaataaaagg aattaagaat
    12841 cccgcactta aaaaaaaggt gaggacggta gcttttaaaa gacttaagag tctagcgatt
    12901 ttttggtccc aaaaataaaa agaaagaaac aacgttcctc caataaatac agaaaccata
    12961 gcgacagaaa gtaaatgagt atacactact agcgtcatgc caatagacaa ccagtaccat
    13021 ttttggtaat ctctaatata aatttcgtaa caccccatta gtatcaaagg caaaaatgtt
    13081 aaagcgacag cttctcccaa tgaagcacga tggaatatgt caatggcacg ataggcggaa
    13141 aatgtataaa taatcgaaaa taacagcgaa atataacgat tgttttttat ttgtttcata
    13201 gaaaaataag
//
LOCUS       DACCXM010000074        13038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCXM010000074 DACCXM010000000
VERSION     DACCXM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.076"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(79..151)
                     /locus_tag="IVY61_001854"
     tRNA            complement(79..151)
                     /locus_tag="IVY61_001854"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Lys,seq:ctt)
     gene            532..1101
                     /locus_tag="IVY61_001855"
     CDS             532..1101
                     /locus_tag="IVY61_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP7970966.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1088..1948
                     /locus_tag="IVY61_001856"
     CDS             1088..1948
                     /locus_tag="IVY61_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7970967.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2138..2842
                     /locus_tag="IVY61_001857"
     CDS             2138..2842
                     /locus_tag="IVY61_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7970968.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2847..4682
                     /gene="walK"
                     /locus_tag="IVY61_001858"
     CDS             2847..4682
                     /gene="walK"
                     /locus_tag="IVY61_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP7970969.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4679..5995
                     /locus_tag="IVY61_001859"
     CDS             4679..5995
                     /locus_tag="IVY61_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970970.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5996..6865
                     /locus_tag="IVY61_001860"
     CDS             5996..6865
                     /locus_tag="IVY61_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970971.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6920..7729
                     /locus_tag="IVY61_001861"
     CDS             6920..7729
                     /locus_tag="IVY61_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7970972.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7832..8461)
                     /locus_tag="IVY61_001862"
     CDS             complement(7832..8461)
                     /locus_tag="IVY61_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP7970973.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8631..9923
                     /locus_tag="IVY61_001863"
     CDS             8631..9923
                     /locus_tag="IVY61_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7970974.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9999..10661)
                     /locus_tag="IVY61_001864"
     CDS             complement(9999..10661)
                     /locus_tag="IVY61_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7970975.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10886..11170
                     /locus_tag="IVY61_001865"
     CDS             10886..11170
                     /locus_tag="IVY61_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP7970976.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11221..12846
                     /gene="groL"
                     /locus_tag="IVY61_001866"
     CDS             11221..12846
                     /gene="groL"
                     /locus_tag="IVY61_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP7970977.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4336 a         2252 c         2822 g         3628 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
      181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
      241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
      301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
      361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
      421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
      481 ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa
      541 atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg
      601 gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat
      661 ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat
      721 tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta
      781 gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa
      841 gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat
      901 gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa
      961 aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt
     1021 gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag
     1081 aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgtgccaga
     1141 agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca
     1201 ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt tgccagagga
     1261 aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa
     1321 aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata
     1381 taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac
     1441 gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga
     1501 aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt
     1561 tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc
     1621 ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat
     1681 ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta
     1741 cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc
     1801 gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca
     1861 aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg
     1921 tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca
     1981 gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg cactttcatt
     2041 ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg
     2101 agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga
     2161 tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgatgt
     2221 ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt
     2281 gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg
     2341 caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt
     2401 cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt
     2461 agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga
     2521 ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt
     2581 gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt
     2641 ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta
     2701 tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat
     2761 cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt
     2821 gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca
     2881 attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg
     2941 cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc
     3001 aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag
     3061 atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata
     3121 tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg
     3181 aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac
     3241 gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt
     3301 ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat
     3361 attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta
     3421 tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga
     3481 gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac
     3541 aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag
     3601 ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag
     3661 acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt
     3721 ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata
     3781 tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc
     3841 gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc
     3901 gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag
     3961 aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac
     4021 cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg
     4081 aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga
     4141 tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat
     4201 atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa
     4261 atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag
     4321 tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt
     4381 caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac gtggttttaa
     4441 gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt
     4501 tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta
     4561 tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa
     4621 aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat
     4681 gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata
     4741 cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac
     4801 aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct
     4861 tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta
     4921 ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg
     4981 agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa
     5041 tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa
     5101 cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa
     5161 aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa
     5221 ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac
     5281 agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaaaaagta
     5341 tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc
     5401 agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac
     5461 aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa
     5521 agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt
     5581 agggaacctc agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga
     5641 aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga
     5701 aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat
     5761 accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc
     5821 taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt
     5881 caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa
     5941 ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga
     6001 tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt
     6061 cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga
     6121 atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga
     6181 gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa
     6241 ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt
     6301 tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat
     6361 tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta
     6421 tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca
     6481 agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa
     6541 aaacgataat gattatagca tcacgaaata catgcagacg catttatctg atatcgagca
     6601 gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa
     6661 taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct
     6721 gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga
     6781 taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga caaacaatac
     6841 tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa
     6901 gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc
     6961 aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga
     7021 tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga
     7081 ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt
     7141 cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga
     7201 accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag
     7261 aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca
     7321 attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg
     7381 cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca
     7441 tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg
     7501 agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa
     7561 tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc
     7621 tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga
     7681 aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc
     7741 aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt
     7801 atttttttag catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct
     7861 tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga
     7921 tcgtactgtc gccagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg
     7981 tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt
     8041 tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg
     8101 catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag
     8161 aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg
     8221 acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa
     8281 catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa
     8341 catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca
     8401 atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca
     8461 taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga
     8521 atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag
     8581 ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt
     8641 ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg
     8701 ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct
     8761 tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggattttcta
     8821 tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg
     8881 ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag
     8941 ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga
     9001 caggtgctga agggatacta ggagcagtta caactgggct catcatcgga tggggctatg
     9061 cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt
     9121 ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt
     9181 cagcggtttt tcaacagttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc
     9241 agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg
     9301 ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc
     9361 ccgtcttgca agcgaattca ttagcgaatg cagaaatctt agcctcagga caagagttga
     9421 cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta
     9481 cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga
     9541 aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa
     9601 ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac
     9661 cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt cctttattta
     9721 cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag
     9781 tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat
     9841 ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag
     9901 cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa
     9961 tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca
    10021 gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag
    10081 atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc
    10141 agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa
    10201 aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg
    10261 atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg
    10321 atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca
    10381 gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt
    10441 ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa
    10501 aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca
    10561 gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca
    10621 tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg
    10681 gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat
    10741 aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag
    10801 aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa
    10861 aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga
    10921 agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga
    10981 gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga
    11041 aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac
    11101 agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat
    11161 tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc
    11221 atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat
    11281 aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa
    11341 aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa
    11401 ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca
    11461 aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc
    11521 gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa
    11581 ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa
    11641 gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt
    11701 gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg
    11761 atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa
    11821 tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc
    11881 acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa
    11941 caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt
    12001 gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc
    12061 gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact
    12121 gacgatctag gtttagaatt gaaagatgta acaatcgaaa acttaggaaa tgcttctaaa
    12181 gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc
    12241 gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc
    12301 gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt
    12361 gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct
    12421 acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt
    12481 atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt
    12541 gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca
    12601 gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga
    12661 gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc
    12721 ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat
    12781 aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggtatg
    12841 atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac agataaaaag
    12901 aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga aattgctttt
    12961 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gctgtttata
    13021 cgtgtttaga gcgtgaga
//
LOCUS       DACCXM010000075        12836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCXM010000075 DACCXM010000000
VERSION     DACCXM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.077"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            350..1480
                     /locus_tag="IVY61_001867"
     CDS             350..1480
                     /locus_tag="IVY61_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7970978.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1572..2327
                     /locus_tag="IVY61_001868"
     CDS             1572..2327
                     /locus_tag="IVY61_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7970979.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2385..4424
                     /locus_tag="IVY61_001869"
     CDS             2385..4424
                     /locus_tag="IVY61_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP7970980.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4533..5684)
                     /locus_tag="IVY61_001870"
     CDS             complement(4533..5684)
                     /locus_tag="IVY61_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7970981.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5838..6368)
                     /locus_tag="IVY61_001871"
     CDS             complement(5838..6368)
                     /locus_tag="IVY61_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970982.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6467..6817)
                     /locus_tag="IVY61_001872"
     CDS             complement(6467..6817)
                     /locus_tag="IVY61_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP7970983.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(6974..8503)
                     /gene="ahpF"
                     /locus_tag="IVY61_001873"
     CDS             complement(6974..8503)
                     /gene="ahpF"
                     /locus_tag="IVY61_001873"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP7970984.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8513..9076)
                     /gene="ahpC"
                     /locus_tag="IVY61_001874"
     CDS             complement(8513..9076)
                     /gene="ahpC"
                     /locus_tag="IVY61_001874"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP7970985.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9220..9882)
                     /locus_tag="IVY61_001875"
     CDS             complement(9220..9882)
                     /locus_tag="IVY61_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7970986.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            10066..11712
                     /locus_tag="IVY61_001876"
     CDS             10066..11712
                     /locus_tag="IVY61_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7970987.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11703..12785
                     /gene="uxuA"
                     /locus_tag="IVY61_001877"
     CDS             11703..12785
                     /gene="uxuA"
                     /locus_tag="IVY61_001877"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7970988.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         4235 a         2373 c         2463 g         3765 t
ORIGIN      
        1 aaagtctatc atgaactcgt aaatagaata tagtaataac cttgttaggt gaggctccta
       61 tacaaacata ggctgttgcc cagaaacgtc gaaagtcgcc aatgggtaaa acagaaactg
      121 ttggattaag gctttttcca atagctaaga atgttttctt tacgttgtat agtgctaaaa
      181 ctcggacgat gagatgatgc taggtcagaa ttcttgttcg aaaaggtttc tgaccttttc
      241 tttatatttt tgtgactgac ttatttgatg agacaaactc gctcctcacc tgacaagtgg
      301 attgctgtgt gaggaggaga tgcggtgaaa aaagatcgat tggtggattt tgggtcctta
      361 tttgactttg tccatgatcg gtcttttaga agtatacagt gccagttctt atcgtttatt
      421 gcaagccgat gaaaatacaa aaagtctatt attgcgtcaa ctaattttca tatttttgag
      481 ttggagcgtg atcttcttag ctcgttcagt caaactacac tatttacttc accctaaaat
      541 agcaggatac ggtttagcct tatcgatttt ctttttagta ttagtaagaa tagggatatt
      601 cggtgtcact gtcaacggcg cacaacgttg gatctctctg tttggcattc aattccagcc
      661 ttctgaactg gcaaatcttt ttttgatttt ttatttaagc tggttttttc gtgacggaaa
      721 tagtagccca aaagatctaa aaaaaccatt cctgattaca gtaggtataa cttttctgat
      781 tttatttcag ccaaagattg ctggagcatt gatgatcctt tcgattgcgt gggtcatatt
      841 ttgggcagcg gcggttccat ttaaaaaagg gatctatcta atcgttactt tttctgcatt
      901 gctgattgga gcagcaggcg gggtattata tttaggaaat aaaggttggc ttccacagat
      961 gtttaatcat gcttatgaaa ggatcgcgac tttaagagat tcttttatcg atagtcatgg
     1021 tgcgggctat caaatgaccc attcgtttta tgcactatat aatggtggca tatttgggcg
     1081 gggattagga aatagtatca caaaaaaagg gtatcttcca gagacagaaa cagattttat
     1141 tttttcgatt attacagaag agcttgggct aatcggtgca ttgtgtgtgc ttttcttatt
     1201 gttctcatta tgcatgagaa tcttttgttt aagcagtcgc tgtaaaaatc agcaagctgg
     1261 attgtttctt ttaggattcg gaactttgct attcgttcag acgataatga acgtgggaag
     1321 tatcgcgggg ctgatgccaa tgactggtgt gccattgcca tttgttagct acggaggaac
     1381 aagttatctg atcctttctt taggaatcgg aatcacgttg aatatttctt caaagataca
     1441 ggccgaagag ttggcgctct atcgacctga aaaacaataa agagaaactg agaaactctc
     1501 agtttgaatt atcataaagt acaatctttt atttttcaag cgagaggttc ttttagaaag
     1561 aggaattcgc aatgaaagta tctcaaaaaa tcatattatc tattttgtta ctgcttacat
     1621 tagtagttgg ctatttttcg attgagttta tccatggatt ttcgtctgca aaacagagtt
     1681 caactgtgaa aaaggtagag ccgaaaagta tccctaccac actaaatgtg gctttgattg
     1741 gttcagatgc tcgttcgaaa gacgaaaacg gtcgctcgga ttcacttctg gttgcacaat
     1801 acaaccagaa aacacaacaa gcaaaactga tctctatcat gagagattca tatgtcgata
     1861 ttccaggtta cggaatgaat aagataaatg cagcttactc ttatggggga gttgatttat
     1921 taaatcaaac attaaaggaa aatttcaaat ttgaagctcc ctattatgca agtatcacat
     1981 ttcaagattt tatcgattgc gttaatgaac tgtttcctgg tggagtcaag atcgatgcag
     2041 aaaaatctct agatttagat ggcgtatata taaacaaagg agagcaagtg atggatggta
     2101 atactttact ccagtatgct cgatttcggg aagacaaaga aggagacttc gggaggatca
     2161 gaaggcagca gcaagtgatc aaagctatct ctcagcagct gaaagacgta acttcaatat
     2221 tcaagctacc caaagcggtt gggaaattac ttggtagtat ccaaacgaac cttccagaaa
     2281 gtgtgcttct ggattgtggt atggatttcc tcaaagacat atgttagaat aaacaggtat
     2341 aaatagtgaa ataaaggaat gacaagcaag agaaggagga aaaaatgaaa agaagtgaca
     2401 agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc agtgatctta atagcagctg
     2461 cgggtggcgg ttatttttac taccagcact atcaagaaac ccaagcagta gaagctggag
     2521 aaaagacagt tgagcaattt gtccaagctt tgaacaaggg agattataac aaagctgcag
     2581 aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc tgaaaaagag atcttagata
     2641 aataccagaa tatatacggt gctgctgatg tcaaaggact tcagatatcg aatctaaaag
     2701 tagataaaaa agacgattct acttacagtt tttcatataa agcaaagatg aatacctcat
     2761 taggtgaatt gaaagatctt tcttataaag gaacattaga cagaaatgat ggtcaaacca
     2821 cgatcaactg gcaacccaac ttggtttttc cagaaatgga aggaaatgac aaagtaagcc
     2881 tgaccacaca agaagcagca agagggaata ttatagatcg aaatggtgaa ccattagcaa
     2941 caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa acttggagat ggaggcgaaa
     3001 aaacagccaa tatcaaagcg attgcttcct ccttcgactt aacagaagat gctatcaatc
     3061 aggcgatttc acaaagctgg gtacaacccg attactttgt cccattgaaa atcattgatg
     3121 gagcaacgcc agaacttcca gctggagcta ccattcaaga agtagacggc agatattatc
     3181 ctttgggtga agcagctgct cagctgattg gttacgtggg agatatcaca gcagaagata
     3241 ttgataagaa tccagaatta agcagtaatg gaaaaatcgg tcgctcaggt ttggagatgg
     3301 cttttgataa ggatcttcgt gggacaacag gtgggaaatt aagcattaca gatgcagacg
     3361 gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa tggcaaagat atcaaattga
     3421 caatcgatgc gaaggcacaa aaaacagctt ttgacagtct aggaggaaaa gctggatcaa
     3481 ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct tgctagctct ccaagctatg
     3541 atccaaacaa aatgacaaac gggatctcac aagaagatta caaagcttat gaagaaaatc
     3601 ctgaacatcc attcatcagc cgatttgcga caggttatgc tcctggatct acgtttaaaa
     3661 tgattacagc agcaatcggt ctcgacaacg gcactatcga tccaaatgaa gtgttgacga
     3721 tcaacgggct taaatggcaa aaagacagtt cttggggatc ttatcaagca acgcgtgtca
     3781 gtagtgatgt atcacaagta gacttaaaaa ctgctttgat ctattccgat aatatatatg
     3841 cggcacaaga aacgttgaaa atgggtgaga aaaaatttcg tacaggctta gataaattca
     3901 tttttggtga agaccttgat ttgccaatta gtatgaatcc agcacaaatt tctaatgaag
     3961 atagctttaa ctcagatatc ttgctagctg atactggata tggacagggc gaacttctaa
     4021 ttactcctat ccagcaagca gcaatgtatt ctgtttttac caacaatggc acacttgtct
     4081 atcctaaatt gattgcagat aaagagacaa aagataagaa gaatgtcatc agcgaaacag
     4141 cagtacaaac gatcgtgcca gatctgagag aagttgtgca agatgtaaat ggtacagcac
     4201 attctctttc tgctttaggg attccgttgg cagcgaaaac tggtacagcg gaaatcaaag
     4261 aaaaacagga tgtaaagggg caagagaaca gtttcttgtt tgctttcaat cttgataacc
     4321 aaggttatat gatggtcagc atgttggaaa ataaagaaga tgatgattca gcaaccaaac
     4381 gagcatcgga actattgcaa tacctcaacc aaaattatca ataaaaaatt gctttttaag
     4441 tacagtaaaa ggactgtggc gttatcgtca cagtcctttt ttcaaataaa cggttttcac
     4501 gatgtctctt tcacaacttc tttttgattt actcacactt cataaacttt ttttgtaaaa
     4561 tatttgagaa caagtggaga aataagtgta cttaataaaa tcacgataac taaaggtgaa
     4621 tagtactgtg gttcgatcaa ttgactttgc tgtccaattt gtaaaatgat caaagccatt
     4681 tctccacgcg aaatcatacc agcaccgacc atcaatgaac tatgttgaga aaaaccggca
     4741 atctgagcac caaaataacc tccaagcaat ttggtcaaaa tcgctacaag cgtcaatata
     4801 gaaataaaga gtagttgttc actaaagtca gaaaaatcaa cttctaatcc tacactgaca
     4861 aaaaatactg gtataaatac tgcatatcct aaagcctcta cattgtggta tacttcttct
     4921 ttaacctttg tctgactgac agctatccca gcaaaaaaag caccaatgac agagctaagc
     4981 ccaacaagat ccgctacgta agacattcct aaacaaataa ctagcgatac gatgataata
     5041 gaagaatttg cgtacacttt ttctgcaaat gaaagtagga aaggcgcaat ccatttgact
     5101 aataagaaaa taaaaacaaa ataaattagc tgttcaatca aaacaagcgg taaagaagca
     5161 ttgctagaag cttcccccgt tagaaaagat aggctgaaac ttagaactaa aacgactaaa
     5221 atgtcatcta ccacagaagc acccaaaatt gttgatcctt cttttgtatt cactacgttt
     5281 agttctttta atacttctac tgaaatactg acagaagttg ccgcaagaat aatcccaaag
     5341 aaaatcgctt catttcccga aacttggaat gctttgcctg tcaaccagcc aaaaacaacc
     5401 gggaagatga tcccaagcaa agcaacgaac ataccaggtc ggaaatattt tttcaaaaga
     5461 gataaatcac tttccagacc agctaaaaac attaataaga tcacaccaat ctccgaaaag
     5521 tcatggacta gaatatcagg tttgacccaa ttcaatccag cttggcctag caaaacacca
     5581 acaagtaatt gtccaatcac tgctgggata cctaatctcc gcgagatatg tgagccaatc
     5641 gttgttgaaa tcaatatcag gcataaaata ccaataaatt ccattctcat cattcctttg
     5701 tataaaatat agcccgaact tagcataaca tacattattg aaaaaatagg aaaatatact
     5761 cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt tggtttttat acggtctatt
     5821 ttaataacta aaaatgatta acgtatagtc atttttctta ttctgatgaa attgataatc
     5881 tcctggatgc ccatgaaaat caaaataaag ccgaatattt ggaatagaag catcaaactt
     5941 ttgaatggat tgaacaataa gacgaatccg ccaatcaaca acagaatact actataaaca
     6001 ctccaacttt tagcttttgt attcatgctg aagaacaact ggaacaagct gttgatgata
     6061 acagctaatc caagaagaat cggcaggata gaaacgattg caggtgcaaa agcatagacg
     6121 atcagagcta agatcaggaa aaaaagacca ctgaacagac cgatactcca agaacctgtt
     6181 ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc caagcaaaac aagataggcg
     6241 gtgatcagat aaccgataaa atgaaaaaca gcagaagggt ttagcacaat cgcaatccca
     6301 gaaataatat aaacagcagc tcttaataag gcgtattttt gaatattttg tttaatcgtc
     6361 tcaaacatat aagaaaacct cctttgactt attttaacgt gaactagtca ttttaccaaa
     6421 ttaaatgtta caaaatgagc gaaggagaag aatatctctg gaaaaatcaa cttctgagga
     6481 tcttttccat ttttttgcct tttgctagct catcgatcat tttatctaaa taacgaattt
     6541 ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc acaaacaaca cctgtaatca
     6601 aggaacgtga aggattgggg acaggggctt ccgcaaaaaa ttgttcgaaa gtcacttgtt
     6661 tttgtagttg atattctagt tctgtttggg aataacccgt cagccaacaa atgatttcat
     6721 ctacttcctc ttttatacga tcttttctct ccgcttttgc tacatacata ggataaacgc
     6781 ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat cagtcacctt tctttttttc
     6841 aaattattta ctacttatag tatataagaa aaagagaaga aaaagcgatt attcaaaaaa
     6901 acaaaaagga ttgtgacaaa attcttgtca ccatcctttt ttccgctctc tgacttttta
     6961 caatcttcat agctcaagtg cgcatcagat aatcgaatgc tcctaaagct gcagtagctc
     7021 ctgaacccat tgaaacaatg atttgtttgt atggactatc tgtacaatca ccagctgcga
     7081 aaacgccggg gatattcgtt gcgccatttt tctcggtcac tatttcattt cttggattca
     7141 tttcgatcgt tccatctagc caatccgtat tagggagtaa gccgactaga atgaatactc
     7201 ctgaaacagc tagtgtatgc atctccatcg tacggcgatc gatataggag agagattcta
     7261 cctgattcgt accgctgatt tctttcgtcg ctgcatgttt gataacagtg acattcgcaa
     7321 gagagactag tttatcctgt aagatttgat ctgcttttaa ttctggtaag aattctagaa
     7381 catatacatg ttttgctaac ccagccaaat cgattgctgc ttcaacacca gaattaccac
     7441 caccgataac agcaatctct tttcctttaa ataaagggcc gtcacagtgt gggcaataag
     7501 caattccttt atttttaaac tctttttcgc ctggtacatt gatagaacgc cagtgcgcac
     7561 ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc gttagctaag ccaatattaa
     7621 taaaatgttg ctctttattt aaggaaacgg cctgttgatt catcataatg tcgatagggt
     7681 aactacgcac atgactttct acttgctgca tcaattttgg accttctgtg taagaggtac
     7741 caatcatatt ttcaatccct agtgtttcaa cgacttgtcc gccgaaagaa tcagcgacaa
     7801 gtcctgtacg gattccttta cgtgctgcat agatagctgc actactagct gctggtccac
     7861 cgccaatcac taatacatca taaggttctt tttcaaacaa ctcttcttca atcaaagggc
     7921 cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc gctactaaat tcttctccat
     7981 ttaaaaacac agttggcact gccatgattt ttttatcatc gatttctttt tggaacatcc
     8041 caccttcaat catcgtatga gtaatatttg gatttaatac agctaatata ttcaatgcct
     8101 gtacaacgtc ggggcaatta tgacaagtca agctaacata actgtcaaaa tgcaatgttt
     8161 gtgttatttt tttgatttta agactcaaag attcactgat tttcggtgcc cgtccactaa
     8221 tttgtaacat agccagtaaa aaagaggaaa attcatgacc gagggggata ccagcaaaaa
     8281 cgattccact ttctttgcca actggattga tagaaaaact aggtgtgcgc atcgaatctc
     8341 ttttttccca agagatttta ggagacattt ctgctatttc cttcgtaaat tcagctagtt
     8401 tttgactatt ttcactatta tccaaactaa cagaaaaaac aataggagat tcaatcagtg
     8461 ctaaatattg ttcgagattt tgtttagttt ctttgtctaa catatccacc acctaaatct
     8521 ttccaacaag atcaaaactt ggtttcaatg tttcgccact ttctttccat tttgccggac
     8581 aaacttcacc tgggtgggta cgtacatatt gagcagcgcg aatcttatca atcagtgcac
     8641 tagcgtcacg accgattcca tctgcattga tttccatagc ttgaacgatt ccgtctggat
     8701 caataataaa tgttcctcgc tgagctaatc cttgctcttc atccaaaaca tcaaaaatac
     8761 gagaaatctg atgagacgga tcaccgatca tcacgtattc gattttaccg atagcatctg
     8821 atgtatcatg ccatgctttg tgagtaaagt gggtatcggt agagcaagaa aagacttcaa
     8881 cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc ttctaattca gtaggacaaa
     8941 caaaagtaaa atcagctgga tagaagcaaa gaatactcca gttccccata agatcttttt
     9001 cactgacttc taaaaattct ccttgatgat aagcatgtgc attaaattct tcgatttttt
     9061 tcccaatcaa tgacatagta aaacctccaa taataattat ttatttataa ttattgtaat
     9121 ttagttggag ttgcaaagtc aatggatatt ttgttattta ataaagatta ctcaaaaacg
     9181 aaggttaatt ataaaaatag aagaggtcgc aagaaaatgc tactgctgga aatactcggg
     9241 ataacgttga gtaacagttt cctgttcttt cagcatcatg tgtaagtgca atcccgtttg
     9301 aaatcgagct tcttctggtt ttttatcaac taaagccttg agtaaatcat aatgttgttc
     9361 catgatcgtt tcccatggaa gcccctgaat ggtcactcgt aaccagcgga aacgttctaa
     9421 atgggtatta tggtcatcta accagttcca aacttctttt cttcctgcaa tttcgaaaca
     9481 agcttcatga aataaattat ctgcacggaa aaaagcgcgt tcgtcattta ttttgactgc
     9541 tttttcttgt tcttctaaaa tcgtttctag cactattcgt gtttgtgtgt ccaatttgga
     9601 acaacattca atcatgattt gctcttccaa ctgttctcgg acaaagcgag cattttctgc
     9661 agaaacaaga ttaatttttg aaacatacgt accactttga ggtatcgtat acacgagttc
     9721 ttgctgacgt agttgaatca atgcttcgcg aatgggagtc cggccgatag caagatgttc
     9781 ctccagtgcc ttttcagata ttttccttcc aggttctaag tcagcataga taatttgttt
     9841 acgaatcgct tgataagctt gagattgcaa gctgcttttc atctttattc ctccaaaatc
     9901 atttctatga acgtagtata acagaagagg tctgaaaaac aatctggtat gtcacattaa
     9961 ctagtaaatt tgtataaata gtaaaatttt ggttgacata gcgattttta ttgcataaaa
    10021 ttcacttgaa tacaaaaact aatatgttag tttaggaggg gaaaaatgat tgatttaaac
    10081 aactggagga actttatccc tgaattatct caaaaagaag tagaaatacc tcattttgac
    10141 cgtgcatcat taagagaaaa tggacaaaaa aatcctgtct ggatccattt cggtggcgga
    10201 aatctttatc gaggatttca tgcagaaatc gctcaacgtt tagcggatct aggcgaattg
    10261 tccactggtg tcattgtttg tgaaacattc gatgaagaaa taattgaaag ggcttaccaa
    10321 gattttgatg atttgattct tgaagtggtc atgccaagca gtggagagtt cgtcaaacgt
    10381 atattagctg cgacagctga cagtctgtat tgtcatccag attgttctga acactttgat
    10441 aagatgattc gacactttga aaatcctagt ttacaattag ttacttttgc tattactgaa
    10501 aaaggttatc gattgaaaga tacaaataat aaatatatag gaccgattgc agtcgatata
    10561 aaaaatggtc caaagaagcc atcacataca atgagtattc ttgctgcgct actttataaa
    10621 cgttttttat ctggagcttg gccgattgca cttgtctcta cagataattt ttcacaaaac
    10681 ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag aatggcaaaa ggaaggttat
    10741 gtacaaaata catttatcca atacttatcg aatgaagaaa ttgttgcgtt tccatggacg
    10801 atgattgatc gtatcacccc gaatccttcc ttgtcagtta gcaaaatgtt ggcaaaagag
    10861 ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa ataatattgc tccatttgtc
    10921 aatacagagg acatccatta tttaatcatg gaagatcatt ttcccaatgg acgtccgaaa
    10981 ttcgaaaaag ctggagtatt cttgactgat cgtgcaacgg tagataaagc agatacgatg
    11041 aaggtgacga cttgccttaa tcctttgcat acgtctttag ctattatggg ttgtttactg
    11101 gggtatagga gcatagctga ggagatgaaa gatccagatt tatcgctgct agtttctgaa
    11161 atcgggtaca atgaaggcct cccagttgtg gagtctccag gtatcattga tccaaagatc
    11221 tttctggaag aagtaattac tgtgcgcttt tctaatccaa atattcctga tatgcctcag
    11281 cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt atggtgagac gattaaaaaa
    11341 tatatggaaa tagaagttaa agacccgcag acacttgtat tcataccatt gactctcgct
    11401 gcttggcttc gataccttat tggcattgat gatcaaggaa aaccattcat cccgagtcca
    11461 gacccactat tgacagactt tcagcataag ctttccgata ttcaactggg tacgcaagat
    11521 acatcacata ttcatgagaa aatggctcct attttgtcaa ataaagaaat ttttggtgta
    11581 gatctttacc aagcagagct tggcagaaaa atagaagaaa tgttcatcaa gatggtagca
    11641 ggaccgaatg ccgtacgaaa aacattacag gattacttac aagaacatgg aggaaattta
    11701 tcatggaaat gacttttaga tggtacggat cacaggaaga aaagattacc ttagatgaaa
    11761 tcagacagat tcctgcggtc aaagggattg taggcacatt gatggatata ccagtaggag
    11821 aggtatggcc tagggagagg atcaaagaac taaaagaaga aattgagtct cgtgggttaa
    11881 cactaaaagt aattgaaagc gtcaatgtgc atgatgccat taaaattgga ttaccttccc
    11941 gtgagcagta tatcgaaaac tataagcaga caatccgaaa cttggcagaa tatggaatag
    12001 aagtcatctg ctataatttt atgcctgtat ttgactgggt gaaatctgac cttgactacc
    12061 gcttagaaga cggctcatcc acgttagctt ttatatctga tgatattcca gatgatccaa
    12121 gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt tgaattgccg ggatgggaac
    12181 ctgagcgctt agctcatatc aaatccttgc ttgaagccta tgcatcagta gacgaagaaa
    12241 aacttcgaga aaattttgcc tattttctcc aatcaatcat cccaacatgt gaagaggtag
    12301 gggtcaaaat ggctgtacat ccagatgatc cgccatactc gatttttgga ttacctcgaa
    12361 tcgtaaagaa tcgagaagat ttagattggt tgtgccgcat agtagacagc ccttataacg
    12421 gaatcactct ttgtacaggg agtattgcag aagatccaga aaatgatgta tatgggattt
    12481 tagcagaatt ttgtcaaaga gatcgaatcc catttgcaca tgttcgaaat attaaatttt
    12541 tagctaatcg agatttctat gaggcaccgc ataaatccga atacggttca ttagacatgt
    12601 accgcatact aaaaacaatg tatgacaacg gatttaacgg atacatccgt cctgatcatg
    12661 gaagaatgat ctggaatgag acaggaagac caggatacgg cttatatgat cgagcattag
    12721 gtgctgttta tctaaatgga ttatgggaag caatcgaaaa aggaaacaaa agtaaagata
    12781 attagtgaaa aaaactgtaa ttttattatg taaataagaa ggatgttcaa attctt
//
LOCUS       DACCXM010000076        12732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCXM010000076 DACCXM010000000
VERSION     DACCXM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.078"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVY61_001878"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVY61_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7970989.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IVY61_001879"
     CDS             complement(322..891)
                     /locus_tag="IVY61_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP7970990.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IVY61_001880"
     CDS             1077..1979
                     /locus_tag="IVY61_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7970991.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVY61_001881"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVY61_001881"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP7970992.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVY61_001882"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVY61_001882"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP7970993.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVY61_001883"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVY61_001883"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP7970994.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IVY61_001884"
     CDS             8547..8732
                     /locus_tag="IVY61_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP7970995.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IVY61_001885"
     CDS             complement(8794..9990)
                     /locus_tag="IVY61_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7970996.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IVY61_001886"
     CDS             complement(10160..11596)
                     /locus_tag="IVY61_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7970997.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IVY61_001887"
     CDS             11889..12698
                     /locus_tag="IVY61_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7970998.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2250 g         4009 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt ga
//
LOCUS       DACCXM010000077        12684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCXM010000077 DACCXM010000000
VERSION     DACCXM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.079"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(287..649)
                     /locus_tag="IVY61_001888"
     CDS             complement(287..649)
                     /locus_tag="IVY61_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7970999.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IVY61_001889"
     CDS             complement(723..1103)
                     /locus_tag="IVY61_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP7971000.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IVY61_001890"
     CDS             1185..2360
                     /locus_tag="IVY61_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7971001.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IVY61_001891"
     CDS             complement(2437..3021)
                     /locus_tag="IVY61_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7971002.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IVY61_001892"
     CDS             3222..4220
                     /locus_tag="IVY61_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7971003.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IVY61_001893"
     CDS             4332..4841
                     /locus_tag="IVY61_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP7971004.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IVY61_001894"
     CDS             complement(4871..5530)
                     /locus_tag="IVY61_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP7971005.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IVY61_001895"
     CDS             complement(5532..6296)
                     /locus_tag="IVY61_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP7971006.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IVY61_001896"
     CDS             complement(6304..6981)
                     /locus_tag="IVY61_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP7971007.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IVY61_001897"
     CDS             complement(7018..8403)
                     /locus_tag="IVY61_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP7971008.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IVY61_001898"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVY61_001898"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IVY61_001899"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IVY61_001899"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP7971009.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9341..10198
                     /locus_tag="IVY61_001900"
     CDS             9341..10198
                     /locus_tag="IVY61_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP7971010.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10279..10614)
                     /locus_tag="IVY61_001901"
     CDS             complement(10279..10614)
                     /locus_tag="IVY61_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971011.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10681..12051)
                     /locus_tag="IVY61_001902"
     CDS             complement(10681..12051)
                     /locus_tag="IVY61_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP7971012.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12129..12665)
                     /locus_tag="IVY61_001903"
     CDS             complement(12129..12665)
                     /locus_tag="IVY61_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7971013.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3768 a         2574 c         2118 g         4224 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga caactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 ctttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact
     9361 tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc
     9421 ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac
     9481 cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatca
     9541 ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca
     9601 cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt
     9661 tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat
     9721 aaaccgtgat tatcttgatg ctactgaaat caacggtatt tttctatctt tagatgctga
     9781 ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt
     9841 ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca
     9901 cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa
     9961 agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat
    10021 cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct
    10081 tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct
    10141 aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag
    10201 atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg
    10261 ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac
    10321 atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt
    10381 gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat
    10441 ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg
    10501 agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac
    10561 actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat
    10621 cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac
    10681 tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc
    10741 aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg
    10801 aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat
    10861 attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt
    10921 gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa
    10981 ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg
    11041 aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact
    11101 tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc
    11161 aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc
    11221 agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc
    11281 aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc
    11341 aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga
    11401 caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat
    11461 tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg
    11521 atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc
    11581 gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat
    11641 ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc
    11701 accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc
    11761 aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt
    11821 tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct
    11881 atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt
    11941 tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag
    12001 acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt
    12061 tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat
    12121 gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg
    12181 tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc
    12241 aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg
    12301 tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat
    12361 tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca
    12421 atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt
    12481 tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat
    12541 gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct
    12601 tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc
    12661 atcattctta cctccgtctc tttt
//
LOCUS       DACCXM010000078        12583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCXM010000078 DACCXM010000000
VERSION     DACCXM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.080"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            47..892
                     /locus_tag="IVY61_001904"
     CDS             47..892
                     /locus_tag="IVY61_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7971014.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1083..2270
                     /locus_tag="IVY61_001905"
     CDS             1083..2270
                     /locus_tag="IVY61_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7971015.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2276..2632
                     /locus_tag="IVY61_001906"
     CDS             2276..2632
                     /locus_tag="IVY61_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP7971016.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2634..2972
                     /locus_tag="IVY61_001907"
     CDS             2634..2972
                     /locus_tag="IVY61_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP7971017.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            3367..4401
                     /locus_tag="IVY61_001908"
     CDS             3367..4401
                     /locus_tag="IVY61_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7971018.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4394..5080
                     /locus_tag="IVY61_001909"
     CDS             4394..5080
                     /locus_tag="IVY61_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7971019.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5104..5952
                     /locus_tag="IVY61_001910"
     CDS             5104..5952
                     /locus_tag="IVY61_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7971020.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            6149..6922
                     /gene="sufC"
                     /locus_tag="IVY61_001911"
     CDS             6149..6922
                     /gene="sufC"
                     /locus_tag="IVY61_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP7971021.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            6935..8221
                     /gene="sufD"
                     /locus_tag="IVY61_001912"
     CDS             6935..8221
                     /gene="sufD"
                     /locus_tag="IVY61_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP7971022.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            8218..9453
                     /locus_tag="IVY61_001913"
     CDS             8218..9453
                     /locus_tag="IVY61_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7971023.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            9440..9910
                     /locus_tag="IVY61_001914"
     CDS             9440..9910
                     /locus_tag="IVY61_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP7971024.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            9915..11306
                     /gene="sufB"
                     /locus_tag="IVY61_001915"
     CDS             9915..11306
                     /gene="sufB"
                     /locus_tag="IVY61_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP7971025.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(11404..12546)
                     /locus_tag="IVY61_001916"
     CDS             complement(11404..12546)
                     /locus_tag="IVY61_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7971026.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4036 a         2361 c         2752 g         3434 t
ORIGIN      
        1 aatagttttt atagtaatta tcataaaaag ttatggaagt ggtgccatgt ttaagttatt
       61 aacgactttt cgagcagtat atgaaacaag aaatttttcc agagcagcag aagtactgtt
      121 tttatctcaa ccagcagtct cgaatcaaat caaacagttg gagcgggaat tgaatatcga
      181 tctttttcaa cgtaacggaa gaaaagagat gcttccaact aagcaagccg acttattata
      241 tgaacggaca ctggttttgt tagaagaatg ggaagaaact cagcaaaaat tgctcaatga
      301 acaaaatgaa gaagaagtat gtcggatcgt tgcttcccac acctttgctg tctatctttt
      361 gcctcagttg atgaaacatc tgatcaaaag gtttcctaaa gtgcattttg agatcgaaat
      421 cacgaattct catgaatcat tagaacaagt agcgaaacat gaatccgatt ttggttttat
      481 cgaaaagcca ttagagactt ccggcgtaca aagatactct ttgatggaag accagctagt
      541 catcgctggg gacccaaaaa gcaaattgtg gcttgtaaga gaagcaaact ctggtgtttt
      601 tcattacacg cagcgttatt ttgaagagat gaatatccaa gataagaaga tgatcgtgaa
      661 aagtaacgat gtaattgttg cgttgctgaa ggaaggaatc ggtcgctcga ttttgtctaa
      721 gcgctctgtt ccagaaaatg tgccaatgac tccgttgaat ttccatcgat atttttattt
      781 catccaacga accaatctgg tttctgatca gctgctagag attgccgaag agatccttcg
      841 atacaatgaa agcacagaaa cttctcacag cgaggagaat ctcagctggt agataattgc
      901 atgtatcaaa aaaagttcta tgatacacta aagataagca gtaggctttt gtactgcatt
      961 tgctcaagca actgcacaaa cggaaagtta atttttccca aatgtctatg gaaagacttt
     1021 ccattatcta actggaccgc tataataatc agcagtatgg tttttaagaa aagggatgaa
     1081 acatggaaaa aaaattaaaa gcttcgaacg ataatcgtat tgattatggc gtgattttac
     1141 cagtctttct gttatgtttg attggtctgc tctcattata tgtagcactt agccatgatc
     1201 cgaacaatcc agcagtcttg aaaggcgtag ggatgcaagt tgtctggtat ttagttggag
     1261 cggcagcaat cgttgtcatc atgcatttga attccaaatg gatctggaaa ctgactcctt
     1321 acctttatgg tttggggtta gtggtaatgg gactcttgct caaattctat gatcagaatt
     1381 tagctgcttc aacagggtct aaaaactggt ttcgattcgg tgcgtttact ttccagccag
     1441 cagagttgat gaagattgcg tacatcttga tgatggcact gatcgtaact aaacataata
     1501 cgcaagtaaa agaacggacg atgaaatcgg acttttggct gattgggaag ctcttgatcg
     1561 tgacaatacc ggttttagca ttgatcatgg cccaagatga ctttggaacg atgcttgtct
     1621 ttcttgcgat ctttggcggg attttcctga tgtctggaat ctcttggcgc atcatcgcac
     1681 ctgtagtaat cttggcagta gtcgttggag caggaacgat cttcttggtt acgactgaag
     1741 gcggaagaga cttgctttat aaagttggat tcaaatctta tcagtttgca cgtattgatt
     1801 catggctgga ccctttccat gatacgtcag gaatgagtta tcagccggcc caaggacttt
     1861 tggcaattgg taccggtggc ttgtttggta aaggattcaa cgtaagcaat atctatgtgc
     1921 ctgttcgtga gtccgatatg atttttaccg ttatcggtga aaacttcggc tttatcggtg
     1981 gtgcattcgt cattttcttg tattttattt taatctatcg tatgattcgg gtgtgtttcg
     2041 atacgaataa tgaattttac gcctatatcg caagcggttt gatcatgatg ctgctgttcc
     2101 atgtctttga gaatattgga gcaaatatcg gattacttcc tttaacaggg atcccattgc
     2161 ctttcattag tcagggagga tcctcgattt tagggaacat gatcggtatc gggttgatat
     2221 tatcgatgag ataccaaaat gaagcacctg caagatcttc aaggaggtaa gaaaattgta
     2281 caccttttac tggtacccga aatgttcaac atgcaaaaaa gccaaagcgt ggttagacca
     2341 gcatggcgtt agttatcaaa ccgttgatat gatcaaagaa gtaccgaagg cctcaacgat
     2401 tgagacttgg ctagaaacga gtcaagtacc aatccgtcgc ctgttcaaca caagcgggat
     2461 gaaatatcgg gaacttggac tgaaagatca agtaggcgag tttacggctt caaaagcgag
     2521 ccaagtctta tcgacagatg gtatgctgat caagcgacca ctgatcttac gagaggacca
     2581 gcttgttgca attggattca atgaaaaaat ttatgaagga gaactgatat aaaatggcag
     2641 gcaagcattt gaagaaaaag gacaaccttt ggatcttatt taatggaaaa gaatactgtg
     2701 ttggtttgac aaatgaagcg caagaagaac ttggagacat cacgtttgcc aatctgccaa
     2761 aagcaggaca atcgctaaaa aaagatgaac ctctgatcga agttgaagcg gaaaaagcgg
     2821 tcaatgaatt tgccagcccg ctaacgggta cagtttcttc tgttaatgat aaaatcggtg
     2881 aagatatcaa tatcttgaat gattcagatg aaatgaatgc gtggatctta agtttcaaag
     2941 atgttgatcc aaaagaattt gacgaattat aaaatcagaa aatcacttga aaaaagttga
     3001 cactgattct tccagttgct ataatcataa gcaatcctat atgaaacgag aaagctttga
     3061 aaagaagagt acatgtcacg gacgtttgaa gagagccctg tttgctgaaa atgggtaacg
     3121 aaaatgagat ggaagatggt cttagagcag agtaagcgag cgtaagcaaa cttactacgg
     3181 gagtgcccgt tacagcgcga cagtatacgg aaagaagcta agtccaagta ctggaagagg
     3241 ctgttattgc gaagtaacgg taaatcaagg tggtaacacg gaagttcaag ctttcgtcct
     3301 tttgcttaag gcaagagggc gaaggctttt ttgtttgaaa aattataaga gaaagatgtg
     3361 aatggcatgt cattaatcca actaacgaat gtcaaaaaga cattcaatgg gaaaaacgga
     3421 aacatccaag ccgtcaatga cgtttcacta aacgttgaaa aaggggatat ttatggaatc
     3481 gtcggctatt ctggcgcagg aaaaagtaca ttagtccgtc tcctgaatgg acttgagctt
     3541 cccacgagcg gcgaagtgat cgtaaacgat caagatatta caaaattaaa aaacaaagaa
     3601 ttacgtacat tccgaaagaa aatcggtatg atcttccagc atttcaacct gttatggtca
     3661 agaactgtac ttgaaaatat ccagctgccg ctggaattag ctggcgtacc aaagagcaaa
     3721 cgtaaagaac gggcagaaga actgttgcgt ttagtcgggt tagaaggacg cggacaagca
     3781 tatccttcac aactttccgg cggacagaaa caacgcgtcg gtatcgcacg agcattggca
     3841 aatgatcctg agatcctgct ttgtgatgag gctactagcg cattggaccc gcaaacaaca
     3901 gaagaagtat tagacttatt gttagcgatc aataagaaat tgaatttgac catcgtcttg
     3961 atcacacatg aaatgaatgt gatccgcaaa atctgcaaca aagtcgctgt gatggagttg
     4021 gggaaagtcg tagaagaagg agacgtattg actgtcttca gacaaccgaa aaaagaagta
     4081 accaaacgct ttgtacaaca agacatcgaa ccagaagctg attcgacaga actattagct
     4141 gaactcatca aagaaaatcc aagcggccgt ttggcaactt tgacctttag cgaaagaaat
     4201 gccaacgagc cagtcatttc ccaagcgatt cgacgctata acatcgatgt caacgttgtg
     4261 tatggaaaga tcaaacaagt gaaagaaggt tcctttggtt cattgactgt cttgatgact
     4321 ggtgaaacca aagaattaga tcaagcagaa gtgttcatca aagaacaagg agtaggaatt
     4381 gaggtgatcc atcgtggata aaagcttttc agaaacatat ctagatttta aacaaatcaa
     4441 cccggaaacg atgaatcagg ctatccttga tacactatat atgacattaa tctcaatggt
     4501 cttagtgacg atcatcggat tagttttggg attgatcctt tactctattg gacgcaaaaa
     4561 caaaccgtat gctcgaatca tttatggaat cgtatcgatc atcagtaata ttttccggtc
     4621 aacgccattt atgatcctga tggttttgat cattccattt actaaagcaa tcgtaggcac
     4681 tatgctaggt gcgcaagcag ccattccagc tcttgtttta tccgcagcac cattttatgc
     4741 ccgtctagta gagattgctt ttcgtgaagt cagccctggt gtactggaag cagcagatgc
     4801 aatgggagca agttactggg aaatcatctg gaaggtcttg atacctgaaa gtattcccgc
     4861 attgatttct ggattaacag ttacaacgat ctcgatgatc ggatttactg cgatggccgg
     4921 cgcgatcggc gcaggtggac ttggaggtct tgcttggcag gaaggctacc agcgtggtaa
     4981 tctaacagtg acattcgttg cgactttaat tattttacta atcgtattta tcgttcaagg
     5041 aatcggtgat ttcttaacta agaaaacaga caaaagataa taggatatag ggggatgtag
     5101 gatatgaaaa agaaaatttt tggatttgca gcagcgcttt tgctgactgt aggattagca
     5161 gcatgcggta acaacagcga cagcaaagat tcagcaaata aagcagacga cacgacattg
     5221 aaagtcggcg cttcaccgac accgcacgca gagattttgg aacatgtgaa accattattg
     5281 aaagatgaag gaatcgatct tgaaatcgtc aaattcgatg actatgtatt accgaaccaa
     5341 tctttggaag aaggcgacat tgatgccaat tacttccagc atattcctta tttgaacaaa
     5401 gaaatcaaag aaaaaggata tgactttgtc aatgccggtg ctgtccacat cgaaccaatg
     5461 ggcttgtact ctaaaaaagt caaagatgtt tccgaattaa aagatggtgc aacaatcatt
     5521 acttcaaatt ctgaatcaga ctggggacgc atcatcacaa ttctacaaga tgctggctta
     5581 gtcaaagtca aagatggagt agatctggaa acagcaacat tcgatgatat tgaagaaaac
     5641 cctaagaact tgaagttcaa ccatacgatc gacccatcac ttttagcttc tacgtaccaa
     5701 aatgatgaag gtgacctagt tgcaatcaac gcaaacttcg cttacggcgc agggttgaat
     5761 ccattagaag atgccgtatt gcttgaaaag gacaactctc cttacgtgaa tattattgcg
     5821 actcgtaaag gtgatgaaga cagtgacaaa atcaaaaaac tgattgaagt gcttcaccgc
     5881 aaagaagtcc aagaatggat cacagacaaa tggggcggct ctgttaaacc agtaaaggct
     5941 gatgcaaaat aaggtatgga acaaaaaaga tgagaaataa gtctgtaatg gcttatttct
     6001 catctttttt atacctattc taatttagaa atcaaaattg tttaataata aataaaagat
     6061 atttgatttt agtaaggaag gtcattgcgc ttcattctca attagtctaa aatgagtgag
     6121 aacgaaagag aatttggggg tataatgcat gtctgtctta gaaataaaaa atctacatgt
     6181 gtcgatcgag gaaaaagaaa tccttaaagg cgtaaaccta acaatgaaaa ccggagaaat
     6241 ccatgccatc atgggaccta acggcacagg aaaatctacc ttatctgccg ctatcatggg
     6301 caatccaaac tatgaagtaa cagaaggcga aattttgttt gacgggaaaa atgttttaga
     6361 cttggaagta gatgaacgtg cgcgacttgg cttattttta gctatgcaat atccaagtga
     6421 gataccaggt atcacgaatg cggaattcat gcgtgcagcg atcaatgcga aacgtgatga
     6481 aaacgacaaa atttctgtta tggcattttt gaaaaagtta gatgaaaaaa tggctttact
     6541 aaatatgcca gaagaaatgg ctgaacgtta tctgaacgaa ggtttttctg gcggagaaaa
     6601 gaaacggaat gagatcctgc aattgttgat gttagaacct acatttgcaa ttttagacga
     6661 aatcgattca ggtctggata tcgatgcttt gaaagtcgtt gctaaaggag taaatgaaat
     6721 gcgcggagac aatttcggtg cattgatcat cacccactat caacgattat tgaactatat
     6781 cacacctgat gtcgttcaca tcatgatgga aggccgagta gtcatgacag ggaatgctga
     6841 cctagctaaa cgtctggaag cagaaggcta cgctggtatc agtaaagaat taggaatcga
     6901 ctataaagaa gaagaagcgt aagggggagc aagaatgaaa gaaaaaaact ggctcgatta
     6961 tcttgacgcg gtaaatgatt tttctttaag taaaggcgaa cctgattgga tgagaacctt
     7021 ccgtcaagac gcactagcga aagcagatga actgccattg ccgcatatcg atcgggtgaa
     7081 attccatcgc tggtcgttat ttgatgtgaa agaaacacaa accatcagtg aaacaggcac
     7141 gattccggca tttgatgcaa tgaaagacaa tcctgtgctt gtccaacaag gttcatggac
     7201 gatttttgaa caactgcctg tggaattagc agaaaaagga gtcattttta ccgacttgtt
     7261 cactgcaatg atcgaatatc cagaactagt ccaagaatat tatatgaaaa aagcagtaaa
     7321 catgaacgaa gaccaattga ctgcactcca tgtagctttt atgaacagcg gtattttcct
     7381 gtatgtgcct aaaaatgtcg tgatcgatga accactcgag tccttattca ttcaagatgg
     7441 cgcatccgat gagcacttct tcaagcatgt gttgatcgtg gcagatgaac atagtgaatt
     7501 ttcttatctg gaacgtttcc agacaaccaa agaacaagta gcaaaatcat caggtaatat
     7561 catcgtggaa gtcatcgcta aagctggttc aaaaatcaaa tattcagccg tagatcaatt
     7621 aggagaaaat attacttctt atatgaatcg ccgtgggcac attttacgag atgcttctgt
     7681 cgactgggca attggtgtca tgaatgacgg tcatgtgatc gctgactttg actcagattt
     7741 agcaggagaa ggtgcacacg cagaagtcaa aatcgtggca atcagctcgg gcagacaaat
     7801 ccaaggaatc gatacacgag taaccaataa agcccctcat acgatcgggc atattttgca
     7861 acacggagtg atccgagaaa aaggaacatt gacctttaat ggtatcggcc acatcttgaa
     7921 aggggcaaaa ggtgcagatg cccaacaaga aagtcgtgtc ttgatgcttt ctgataaagc
     7981 acgtggtgat gcgaatccta ttcttttgat cgatgaaaac gaagtaactg ctggacatgc
     8041 tgccagtgta ggacgagttg atccagaaga aatgtactat ctgatgagta gaggattgca
     8101 taaagaagaa gcagagcgct tagtgattcg aggattcctc ggttctgtct taacagcaat
     8161 ccctgtagaa caagttcgga aagaattagt cgaagtaatc gaagggaagt tgaatggatg
     8221 atcgatgcta aaaagctacg caatgatttt cctatcttat ttcaagacgt caatgatgaa
     8281 cctttagttt atctggataa tgcagcgacg acccaaaagc caaaacaagt actccaggca
     8341 ttagcgtact attacgaaca cgataacgcc aatgttcatc gcggtgtcca tacgttagca
     8401 gaacgcgcga cccatgatta tgaacaagca agagaaaaag tacgggcgtt catccatgca
     8461 aaagaaacag cagaagtgct gtttactcgt ggtacgacga caagcttgaa ttgggtagcc
     8521 agaaccttcg gcgaccgcta tatcaaagca ggagacgaga tagtgctctc ttacatggaa
     8581 catcattcta atgttatccc gtggcagcag ttggcaaaac gaacaggtgc tgtcttgaaa
     8641 tatatcgaac taacagaaga aggcttcctg gatatggagg atgcagcgaa aaagattacg
     8701 gacaaaacca aaatcgtctc cattgctcat gtatccaatg tcttaggtgt cttcaatcct
     8761 gttcaagaat tagctgaact tgtccatgaa aacggcgggg tcatcgtagt agatggtgct
     8821 caatctgccc cgcacatggc cattgatgtc caagcactag actgtgattt ctttgctttc
     8881 agcggacaca aaatgtgcgg accaacaggt attggtgttt tatatggtaa acgaaaatgg
     8941 ttagaagaaa tggaacctgt tgaatttggt ggcgaaatga tcgattttgt cgaattatac
     9001 gacagtacat ggaaagaact accttggaaa ttcgaagcag ggacaccaaa tatcgccggc
     9061 gctatcgcat taggccatgc aatcgattat ttgcaaaaac tcggtatgga caatatccat
     9121 cgatatgaag aagaactagc cgcttatgtg ttgccaaaat tacaagcaat tgatggattg
     9181 actgtttatg gtccacaaga cccgaatttt cggacaggag tcgttgcatt caatttggat
     9241 gggttacatc cccatgatgt cgcgacagct ttagacatgg aaggggtagc cgtcagagca
     9301 ggtcatcatt gtgcgcaacc gctattgaag taccttgaag tatcagcgac tgctcgcgcg
     9361 agcttttatt tctacaacac aacgcaagac gccgaccgtt tagttgaagc aatcatggca
     9421 acaaaggagt ttttccaaca tgggactatc taaattagac aatttatacc gtcaagtgat
     9481 cttggatcat tcttcccatc cgcatcatcg tggaagcttg actgactcga caagcaagat
     9541 cgaattgaac aaccccacat gtggagatgt gatcgaattg caagtcgatg taaaagacgg
     9601 aatcatcaaa aatatcgcct ttgacggcag tggctgctcc atcagtacgg caagtgcctc
     9661 gatgatgaca gacgccgtca tcggaaaatc aatagaagaa gcagaacatt taactgtaga
     9721 ctttctacag cttgtccaag gaaaagaagt caaagatccc gaccaattag gcgatgctgc
     9781 tatgcttggt ggtgtagcta aatttcccgc tcggatcaaa tgtgcgacac ttgcctggaa
     9841 agcactagga aaagcaatgg aaaacgatgg taccgcagct ttccatcatt accacaagga
     9901 agaggagtga gaacatgggc gtaccagaat tagaagaata taaattcggg tttcacgatg
     9961 atgtcgaacc ggttttcagt acaggagaag gcttgacaga agaagtgatc cgtgagatgt
    10021 caagaatcaa aggcgaacca gaatggatgc ttgaattccg tctgaaatct ctagaaacat
    10081 ttaacaaaat gccaatgcaa acatggggcc ccgacctttc agacatcgat ttcgatgcca
    10141 tcaaatatta ccaaaaacca agtgacaaac ccgctcgtga ctgggaagac gtaccagaaa
    10201 aaatcaaaga tacatttgaa aagattggta taccagaagc tgaacgagct tatctagccg
    10261 gtgcttccgc acaatatgaa tcagaagtcg tgtaccataa tatgaaagat gaattcgaaa
    10321 agttaggaat cgtctttact gacactgact ctgctttgaa agaataccct gaattattca
    10381 aagagtactt ttcaaaactt gtaccgccaa ccgacaacaa attagcagcg ctgaattcag
    10441 ccgtatggtc aggtgggacc tttatttacg tgccaaaagg cgtcaaagtc gatgttccat
    10501 tacaaacgta cttccggatc aatgcggaaa acacggggca attcgaacgt acgctgatca
    10561 ttgttgacga aggagcaagc atccactatg tcgaaggttg tacggcacct acttattcaa
    10621 gtaacagcct gcatgccgcc atcgtagaaa tctttacaag aaaagatgcg tattgccgtt
    10681 acacaacgat ccaaaactgg tcagacaatg tgtacaactt agtaaccaaa cgagcaaaag
    10741 catacgaagg cgcaaccgtt gaatggatcg acggaaacct aggtgcaaaa acaacgatga
    10801 aatacccaag tgtttactta gatggaaaag gtgcacgagg caccatgctc tccatcgcct
    10861 ttgcaggagc aaaccaaatc caagatacgg gtgcaaaaat gatccacaat gcaccaaata
    10921 catctagctc tatcgtctct aaatcaattt caaaagacgg cggagaagta aactatcgag
    10981 gacaagtaac ctttgccaaa aacagtgcgg gttcgatctc tcatatcgaa tgtgacacga
    11041 tcatcatgga cgaatggtcg aaatccgata cgatcccatt caatgagatc cacaacagcc
    11101 aagtctcttt agaacacgaa gccaaagtct caaaaatctc agaagaacaa ctctactatc
    11161 tgatgagccg aggcctgacc gaatctgaag caactgaaat gatcgtcatg ggctttgttg
    11221 aaccattcac caaagaactt ccaatggaat atgcagtcga actcaatcga ttgatcagtt
    11281 atgagatgga agggtctgta ggttaataac tatttcattg ctcgaaacgt tgatattaaa
    11341 gcgtttcgag cgttttttta tttgtatttt aatggttggc tacgtttttg gctacgtttg
    11401 gaattattcg cccataaatt ttgcaaatcg gtcaccagtt ttcttaacag attgtggtgt
    11461 gacatgagca tagatattca tggtagtttt aatatcttta tgacctaatc gttcctgtac
    11521 ttcttttatc gacgcaccac tttcaaacaa taagctacag tgggtatgcc taaatccatg
    11581 tggagtaatt tgaggctcca tcggtgtctt tttgtaaatc cagtctaacc aatcgtttac
    11641 tacttggggg tagtacaact tgtttgtgtt tgtagtgaaa agaaactgtt ttggtgaatt
    11701 cgtgttatag ccatataaga tcattatttt catttgttct tgttgccaaa tacgcagttg
    11761 tttaagtgta aaatcatcaa gctgaatcgt tcttgatgaa tttttagttt taggtacttg
    11821 tagaactact tgattaaact cgtcttgagc gatagtttta ccaattgtta ttgttttatt
    11881 gaaatagtct atatcttccc attgaagcgc caatgcttcg cttttacgca ttcctgtata
    11941 agctaataaa cggaaaaaag tgtacagttt aactgaatta ttttctttag tattctgtaa
    12001 aaacagttgt aattgatctt tcgtgtaaaa cttcaagggt tgttcttctt caatttttct
    12061 tggaagcaat gttttagaca taggattatc cttacagact tccattgcta ctccaaattt
    12121 aaatatttga gcagttgttt ttctcaagaa accatattgt tttaatgttt tgtgccattc
    12181 attcacctgt ttttggcaat agctaacact tattttatcc aatcgtaatt ccccaaaagc
    12241 aggtaatata tggtttttgc aaaaacgttg agcaacggca acagaagacg gttttactct
    12301 tgttctgtag ttatcaatcc ataattcata caactcttga aaagttgtaa cattgatttt
    12361 aaactcttgt ttttctttaa agtctacaag tagtctatta agtgctgttt gcgcctcagc
    12421 ttttgatttg aatcttctac gctttgtacg tatatctttt ccagtcttgg gatctttccc
    12481 taaataagtt tcaaatcccc aatatttttc gccattttgt aatttataac taaaaaatgt
    12541 tgccatttga ttctctcctt acttcattat tgcaggtaga gaa
//
LOCUS       DACCXM010000079        12517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCXM010000079 DACCXM010000000
VERSION     DACCXM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.081"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..66
                     /locus_tag="IVY61_001917"
     CDS             <1..66
                     /locus_tag="IVY61_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7971027.1"
                     /translation="DYRFHWNSDNSVIEIWIPIQD"
     gene            194..1408
                     /locus_tag="IVY61_001918"
     CDS             194..1408
                     /locus_tag="IVY61_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7971028.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2118..2306)
                     /locus_tag="IVY61_001919"
     CDS             complement(2118..2306)
                     /locus_tag="IVY61_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971029.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            2397..2606
                     /locus_tag="IVY61_001920"
     CDS             2397..2606
                     /locus_tag="IVY61_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP7971030.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2718..4331
                     /locus_tag="IVY61_001921"
     CDS             2718..4331
                     /locus_tag="IVY61_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP7971031.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            4366..5577
                     /locus_tag="IVY61_001922"
                     /pseudo
     CDS             4366..5577
                     /locus_tag="IVY61_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5600..6526
                     /gene="glsA"
                     /locus_tag="IVY61_001923"
     CDS             5600..6526
                     /gene="glsA"
                     /locus_tag="IVY61_001923"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP7971032.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6734..8194
                     /locus_tag="IVY61_001924"
     CDS             6734..8194
                     /locus_tag="IVY61_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971033.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            8415..10178
                     /locus_tag="IVY61_001925"
     CDS             8415..10178
                     /locus_tag="IVY61_001925"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP7971034.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            10262..10720
                     /locus_tag="IVY61_001926"
     CDS             10262..10720
                     /locus_tag="IVY61_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP7971035.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10829..11512
                     /locus_tag="IVY61_001927"
     CDS             10829..11512
                     /locus_tag="IVY61_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7971036.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            11659..12273
                     /locus_tag="IVY61_001928"
     CDS             11659..12273
                     /locus_tag="IVY61_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP7971037.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            12289..12495
                     /locus_tag="IVY61_001929"
     CDS             12289..12495
                     /locus_tag="IVY61_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971038.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         4328 a         2013 c         2637 g         3539 t
ORIGIN      
        1 gattatcgtt ttcattggaa tagtgataat tcagttattg aaatttggat acctattcaa
       61 gattagaagc aattttatat ccatacacac aaagcagtta gtcttaggat tgactgcttt
      121 ttctatacag atttttaatg acaggcaatt atcaattatg gtagttgcct tttttgattg
      181 gaggaattga agaatgaatg ataaaaactt tattgaagaa ctaagacaaa agcgtgagga
      241 atacggagta acacaaacaa ggcttgctgt tgcctgtggt atcagccgtg aatattacaa
      301 ccgcattgaa aaaggaaaac agccattgaa tgacgaatta agagaaatca tagaaaaaca
      361 gattgagcgt ttcaatccgc aagaaccact attcttattg attgattact ttcgtgtccg
      421 ctttcctact acggacgcat tggcgattat ccgtgatgta ttacaactga aatctaatta
      481 catgctttat gaagattatg gaaaatacgg atatgaaagc aaatatgtac ttggcgacat
      541 caatatcatg tgttccatgc aggagcattt aggtgtttta ttggaactga aaggcaaagg
      601 ctgtcggcaa atggaatgtt atctgctggc acaggaacgc tcatggtatg acttcatgct
      661 ggattgtatg acggctggtg gtgtgatgaa acggcttgat ttggctatca atgataaagc
      721 aggaatattg gatattccaa agttaaagga aaaatacaag gctggcgaat gtatgtccta
      781 cttccgtatg cagaaagatt acagcggtac agaaaaatgc ggtagcgatt taccaaagaa
      841 tacaggagaa accttatatc tcggttcaac aagtagcgaa ttatatatgt gtgcttatca
      901 gaaaaattat gagcagtatg tcaagaatgg cacagaaatt gaagatacgg agattaagaa
      961 ccgttttgaa atacgcatga agaacgaacg agcctattat gcggttgtag atttactgac
     1021 ctatcgggac gctgaacgca ccgctttttc tatcatcaat cattatgtcc gctttgttga
     1081 cagagaggac gacaagccaa aaagtcaatg gataacaaat gatgattggg catggtttgt
     1141 aggggaaaac agagagccga tacgcttaac cacaaagcca gagccttaca ctttacaaaa
     1201 agcgttgcat tggctacaaa gacaagtcgc accaaccata aaaatggtac aggcattaga
     1261 cagagaaaat cgtacaacga tactgaaaga tatgattgag caggcagaac taaaagacaa
     1321 gcataaacat ttattacaat tagagaaatc aaccatagaa gaacgtatag ataccgctgt
     1381 tccacaagag aatgacggta ttttttaatg taattgaaaa atttcagatt tagtcagcaa
     1441 agagctgttt tcattcccta tgtgaagtga aagaagaaaa ataaattttt tattaaatct
     1501 gcaacaaaat ggctacttgc gtacagatat ggtgcgagaa aaggaaatct aaactttttt
     1561 tgaaaatagg tcattaaatc ggggcttgct gtccctatat gaagtgaaag aagaaaaaag
     1621 ttttgaaaaa atgacggaaa ttcgtgatta gtggacagtc ttctgtgaaa agaggaaaaa
     1681 catcatcatt ttgtattcaa acaggcatta gcagttgagt gttgatgtga aaagatgaaa
     1741 atactttgtt ctcaacttaa caatttaagg tttccgttcc ctatatggtg caaagaaaat
     1801 atttcattct attgttggca gtaacggtat ggtttgggta gttagggtgt gaaaaggaaa
     1861 atattctttt ttcatcatca acttagcaaa atgacatttt ctatccctat atggtgcatg
     1921 aaaagaagtt aaaatttttc catttaaact tagcaaatgg acgtttgctg tacagatagt
     1981 agtgaaaaag gagaaaatca accgttgatt gccaaaatga accgttccat ttgtactaat
     2041 aatggaaaaa gttttggatt ttggttacgt ttctcctctt ttccaacgcg atatatagga
     2101 aagacgattt ttttctttca atcgttcttt gacaactgaa taaagctatt caatacgttt
     2161 gacagacagc tagccgttga aacaaatacg ccacgacctt tcccctgcaa gagcgagcga
     2221 aaacctattt gtgttcttgt aaaagatttg tcctctttta tcgccatgac ctgtactgtc
     2281 agataatgat actcccgtac agccacagct tgagcattaa gagcgtcgca cgcaatgggt
     2341 acggctacat aagaactatg cagaggtgga actcctgtgg gctatgacaa aagccgttga
     2401 attgcttaga aagattttac agaaaggggg tatgtgttat tgataatgct gtaaaaacaa
     2461 ttcaaaagaa aaatggcaag tgtggcattg gcaacagagg aaagacaaag attgtagtaa
     2521 aagagcattt ttccgaaaag ggcaaaacaa tggaagaact tctaactgat gtcatgctgg
     2581 aaaaagcaaa gcagacaatc gcataaaatc ggtgcagttg taagaaatag attgaatgac
     2641 aaaaagtacc cgtgttatac tttacttgca agcaggtatt gtaaacacgg gttagttata
     2701 aaggaggata actgacaatg aaacaacaga tttacaatac tgcactttat cttaggttaa
     2761 gccgagatga tgaattacag ggcgaaagtt ccagcattac cacacagaga agtatgttgc
     2821 gtttatatgc aaaagaacat catttgaatg tcattgatga atatattgat gacggctggt
     2881 cgggaacaaa ttttgacaga ccgagttttc aaagaatgat tgaggatata gaggcaggaa
     2941 aaatcaactg tgttgtaacg aaagaccttt cccgtcttgg cagaaattat attatgacag
     3001 gacaatatac agaattgtat ttccctagtc ataatgtccg ctacatagcg attgatgacg
     3061 gtgtagacag cgaaaaaggc gaaagtgaga ttgcaccatt taagaacatc atcaatgaat
     3121 gggtggcaag agatacaagc cgtaaagtca aatcagcatt taagacaaag tttgcggagg
     3181 gggctcatta cggtgcttat gctccgttag gatataagaa acaccctgac atcaaaggaa
     3241 aactgttggt tgatgatgaa acaaaatgga ttattgaaaa aatcttttcc ctagcctatc
     3301 aaggttacgg aagtgcaaaa atcacaaagc agttaagagc agaaaaagtt ccgacagcgt
     3361 catggctaaa ttttacaaga tacggtactt ttgcacatat ctttgaggga aaacccgaaa
     3421 gtaagcgtta tgagtggacg attgctcatg tcaaggcgat attgaaaagt gaggtttata
     3481 tcggaaacag tgttcacaat atgcagtcta cggtatcgtt caagagcaaa aagaaagtgc
     3541 gtaagcccga aagtgaatgg tttcgagtag aaaacactca cgagccgatt attgacaagg
     3601 aagtgttcta tcgtgtgcag gagcagataa aatcaagacg cagacagaca aaggaaaagg
     3661 caacgccgat ttttgcaggg cttgtcaagt gtgcggattg tggttggtct atgagatttg
     3721 gaacgaataa gacaaataaa acgccttaca gttattatgc ttgcagttac tacgggcagt
     3781 ttggcaaagg taattgttct atgcactaca ttcgttatga tgtgctgtat caagccgtat
     3841 tggaacgctt gcagtattgg gctaaggcag tacagcagga cgaagaaaag gtattgaaca
     3901 agatacagaa agctggcaat gcagagagaa tacgggaaaa gaagaaaaag gcaagtacat
     3961 tgaagaaagc cgagaacaga caaaatgaga ttgaccgttt atttgcgaaa atgtatgaag
     4021 atagagcctg tgagaagata acagagcgaa attttgtgat gttgtccagc aagtaccaaa
     4081 aagaacagat agaactggaa cagcagataa caagcctaag agaagaacta agtaaaatgg
     4141 aacaggatat gataggtgct gaaaagtgga ttgagttaat caaggaatat tccgtaccaa
     4201 aggaactgac agcaccatta ttaaatgcca tgatagaaaa aatcctcata catgaagcaa
     4261 caacgaatga ggataacgaa agaatacagg aaattgagat atattaccga tttattggaa
     4321 gagttgagta atcaacaaga gtaatatttt taactaaggg aaaccggcgg tgtctatact
     4381 tgggtcagtc gcacattggg aaaacgattc ggttttgccg caatcttttt ccaatggttt
     4441 gagataacag ttggctactt gaccatgctg tatttcttaa ctggtgcatt atcctatgca
     4501 acgggtattg gtgctataca aaacaataaa tatctgaaat tggcaatcct attgattatt
     4561 ttctgggcta ttttactctc acagttaaaa ggaaccaaat acacttcatt gattgctcgg
     4621 atcggttttg tagctggtat cttgttgcca gcattagtct tgtttggctt agggatccat
     4681 tatgtcgctt caggcgctcc actacaaacg atactgtcta tgaaaacatt gattcctgat
     4741 ttttcgaaat tgcctacgct ggttgttttt gtttccttta ttttagcata tatgggcgta
     4801 gaaacatctg ccagtcatgc aaatgaaatg gaaaacccta aaaaggatta tccattagca
     4861 atgtttgtat tggttattct ggcaatcatt ttagatactt ttggcggatt gaccgtcgct
     4921 actacagtac ctcaacatgg tttgtctctt aatacagggg tgattcaatc gttagaattc
     4981 ctattaagac acattgatcc aaatcttgca tggattgcga aaattcttgc gattcttgtt
     5041 tgtttaggtg tcatcggaga aattgcttct tgggtaacct ctccttcaaa agcacttcac
     5101 gtagcagcag aagaaggatt acttcctgaa tattttgcaa aagaaaacac acacggtgtt
     5161 cctgctcatt tgatgatcgc aaatgggatc gttgcaacaa tctgggcagc cgtctttaca
     5221 ttaagcggcg gggggaataa tctttcattt cttgcagcga tgtcattgac tgttgtaatc
     5281 tacctgatgt tgtactttat gtttttcgcg ggctatttga agctggtttt taaagatgac
     5341 agtcttgaaa gagcctatca ggttccaggc ggaaaagttg taaaagtaat cgttgcttgt
     5401 ttaggtcttt tgacttcact ttttgctttt tgtatctcat ttgttccacc tgctagtatc
     5461 gcagctggac agcatgggat ttatgaaacg attctgattg tttcttatct tatcgtctta
     5521 gttattccat ttacgatcta cgcatgccgt cataaattcc agcatgcaga aaaataaaaa
     5581 atgaaagctg gttattcaaa tggaagtaaa agaattagaa caattgattg gaaaaaattt
     5641 ccactattat caaacaggac aagtggcgga ttacattcca gcattagctg aagtggatcc
     5701 caaacaatta ggtatggcga tttatgattt ggatacaaaa caactgattg agggaggaga
     5761 cgccgatgtg cgctttgcca tcgaaagcat gtcaaaagtt cccgtattgt tattagcaat
     5821 caaagacaac ggaatagaaa aagtctttca aacaatcaac acggaaccaa ccggttttgc
     5881 cttcaattct atcatgaata tggaaatcaa tcatcgcaaa catccaatga atccttttgt
     5941 taatgcagga gcgattgcaa caacctcgtt gattgccggt aaagatgcag atgaaaaatt
     6001 tgagcgaatc ttgtcattta tgaaagagat ctgtgatgat cctaatatca cattgaatga
     6061 agaaatctat cattctgaat caagaacagg tgatatcaat cgctctcttg cttactatat
     6121 gaaaggtaat caaatgatcg aaggagacgt accggagata ctcgatgctt atttcagaca
     6181 atgctccctc aatgtcaccg ctacaggcat agcaaaatta gctgctgtgc tggcaaataa
     6241 aggaattgct ccttggaatg gcaaacaaat cattactgaa gaaagtgcta caatcgtcaa
     6301 atcaattatg acaactgctg gtttgtatga tgaatcagga gagttttccg tccatgttgg
     6361 tgtacctgca aaaagcggtg taggcggcgg gttgatggca gctgtgccaa atcgttacgg
     6421 cataggtgtc ttcagcccgg cactagatcc atttggtaac agcgcagctg gtatccaatt
     6481 gttgaaagat gtagtcaaag aactagacgc tgacatcttt gaataaccat tcttgaaaga
     6541 ggctggggta aaagtccaaa cggctcaaag ctacctacaa tgaggctgaa attgtgagct
     6601 tgtttgactt ttgtcccagt ttcttttttt attggttttt gaaagggctt ttctaaataa
     6661 aaaggaaaca aaaatacgat ttacagatat gattaaataa aatcataaga aaaactggag
     6721 gcgtgtatca ggaatgctgg aaaattacat cgagcgaaat atatttagaa aagtgtatct
     6781 gtgtgaacaa ttatttgaat tccaagaaat agacatcgaa caaacagcta tttctctaag
     6841 ggtgactaca ccaacgatcc ttcacgatct agaaagtcta gccgagtgtc tagaatattg
     6901 tataaaagaa caagtaagag agaagcataa gtataaactc gtattcaaac atggtattgc
     6961 tctttctgag ctcacacagt ttctgtacgg tcaatcttat tttttaaagt ttttaagtta
     7021 ttattttaat ggtatattca cgagtacaga actggctgat cttgaattta tttctttgag
     7081 taaagtatat acaatcaaaa aaatcgtatt ggattttttt aaagaaaatg attatttgaa
     7141 aaataaagaa atcattatcc cagaatttga ctcgcggaac atcttgctag ctttagtacg
     7201 ttatgtcaat tgggaaggct atgaaaataa aaatccgtat gtagaagaag cttgtaacga
     7261 gttgattgat tatgtagaga ctcatttttt taaaagaaga tattcggaag atgaaaagtc
     7321 ctttatcata cgagaaatag agatcgcaat cggaagaaag gcatttcccc ttggactgaa
     7381 agaagaagat aagaaaagcg cagaaacgaa gccgttattt caattagtct gtcaaggatt
     7441 ggaagagcaa aaaggaattc tagacttaca agaagaagat gtctattata ttttttcttt
     7501 gttcaacagt agaaattata ccaatgagaa catggagctg ttaagaaaag atttcgaagt
     7561 tgtttataaa aattttgttc tcaatgacct atcactgaat gaattgattt tacagattat
     7621 tagtatcgct gaaatcgacg atgcagaaaa ttttattttc aagcaatcct ttttacaatt
     7681 tgtccgtacg ttgtgggcag atggacaagt atttttaccg gaaaggatct atcttctatc
     7741 agaaaaagaa caacttctgt acggaaaaat tgtagaaata ttggaagagt ggaaaaagaa
     7801 aaatcaaatc caactaagat ggaatgaaaa tttcattcga aaattcgtca aaagtctttc
     7861 tttgatcaac agtgaacaag cggggagaca ggaaatagaa atttttgttg tttcagaaag
     7921 ttcagtcaaa caattttttt atcgaaccca atttcatttg tatgcggaag aaggcatcgc
     7981 tgaaataaat cctattttat ttcgttcatt agaagaattg cctgatgaat gtctatgtgc
     8041 gaaaaaaaga gtggttttgt gcgatgctgc tagctatcaa aatggattag aaactgaaaa
     8101 gacaaagatc tatccaatct cattaaaagg tgcaaacatc catttgtatc atattgctca
     8161 ggaacttcac ctattaaaaa aacatatttc ttgatgagac cttaattagg agaccttcta
     8221 caaagctttt taattgtctg tagaaggttt ttatttgagt cctgattcgt gcgaagatct
     8281 acttatataa caagctttct ctgaaatttt tttaaaatgc tattgactta gagtcaactc
     8341 taagtagtag aatagataaa gtgtaacaaa tctattttca ttaaaagaag atagtacgga
     8401 ggagagaatg tattatgagt acagtattac atgtattgga tgcaatcaat agccccgcag
     8461 atgtcaaaaa attatctgtt gaggaaatga aacagttggc agaagagatg cgccaaaaaa
     8521 tcttgcaaaa ggacagcgat gtaggtgggc acgttggtcc gaatctaggt gtagtagaat
     8581 taacgattgc gtttcattat gtatttgatt caccaaagga taaaatcgtt tgggatgttt
     8641 cccatcagtc ttatggtcat aagcttttaa ctggtcgaaa aaatggtttt gaaaaaggac
     8701 attaccgtga agtgagcgga tattccaacc aacatgaaag taaacatgac tattttacag
     8761 tggggcacac atctacatct atcagcttag caacaggttt agctaaagcg agagatctga
     8821 agaaagaatc aggaaatgtg gtagcattta tcggtgacgg ctccctaagt ggtggcttag
     8881 cttttgaagg attgaacaat ggtggtgttt tacaatccaa tctaatcgta ttagtaaacg
     8941 ataatgagat gtctattgat gagaatcatg gcagtttata taaaaactta gctgagcttc
     9001 gtcaaaccaa cggtaaaagc agcaataacc tatttaaagc aatgggattt gattatcgtt
     9061 acgtggataa tggaaacgat ctggaaacgg tgatccgttt atttgaagaa gtaaaagata
     9121 ttgatcatcc gattgtactt cacgtccata cggaaaaagg tcgcggttat cagccggcaa
     9181 tcgataataa aatgaaatat cattggcatg taccatttga tctgtccacc ggggaggcaa
     9241 agcaagtaac ttctggagaa acatttaata tgatcatcat ggattatttg gataagaaaa
     9301 tgcaaacaag tacagctcct attgccgcag tcaatgcagc gataccaggc gcctttggac
     9361 tgaaaacatt ccaagaaaaa catccagatc attattttga cgtggggatc gctgaacagc
     9421 actctatcac tttcgcttct gctttggctt ctcagggagt acgtccagtc gtcttccaat
     9481 caagtacttt cttgcaacgg gcatatgatc aattatcaca tgatttagcg atcaacgaga
     9541 atccagtgat tattttagtc ggtggcggaa cgatctcagc tagtgaccga acacaccaag
     9601 gaacatttga cattccttac ttatcaagta tcccgaacat caaatatctt gctccagcaa
     9661 caaaagagga tatgatctct atgctagatt gggcgttcga acaaacagcg cagcctgtag
     9721 caatccgaat tcctacacat ggtgtagaac atggtacagc tatcgcaaat gattatagta
     9781 ttccagccta tcaaacagta aataatggag gaaaagtagc aattttagct ttaggcggtt
     9841 ttcttgaatt aggaaaagaa acagtaaaag cttttagaaa aaaaggaatc gaagcaacgt
     9901 tgatcaatcc aggaacgatc acagaaaatg acgtagaaac attagataaa ttaaaagaaa
     9961 cacacgaatt agtcatcaca ttagaagatg gtagtttgtc aggaggattt ggcgaaaata
    10021 tcagtcgtta ttatggtgat tcttcaatga acgtattaaa ttttggtgca gagaaagaat
    10081 tcacggatag cgttcctgta gggcagcttt atgaacgtta tcatttaaca ccaaaacaaa
    10141 tcgttgaaga tagtctgcgt gtcttgaata ttcaataatt cattatgctc accatccaga
    10201 taattgttta tactagtatc tggatggttt tttcattata aaaagcaaag gggtggatgt
    10261 tatgtatcag caagtagaag aatatgtcaa aaagttccaa gaaaaatatc ctagttatca
    10321 atcagaaaaa ttaggtttct tttcatttgg ggatacagaa aaaatggcag acgagttgag
    10381 cgaactagtt gtaaaaaaga taaagactgg tacatcaagt ggttacgatc tgtataaaca
    10441 agagaatgag cctttaccaa aagttggtca attagacgtg gtcctgaatg gaaaaaatga
    10501 accagtttgt atcatcaaaa atataaatgt gaccatcttg ccttttagtg aagtcacgaa
    10561 agagcatgct tggaaagaag gagaagggga tcggacatta gcttattggc ggaaagtcca
    10621 tgctgagttt tttaaacatg cttataaaga agcgttgaac atagaattta cagaagagaa
    10681 gctggtcgta tacgaagaat ttgaagtaat tttcttttag taagaaaggt ctgctgaaag
    10741 aaatagctca ttattgtctc ctgaatagaa aagggttaca tttttatatg aagctgtatt
    10801 tttatataac attactggag gagatattgt ggatacactg gaacaaatca aaggcggagt
    10861 cgtcgtttct tgtcaagctt tggaaaacga gccgctacac agttctttta ttatggggag
    10921 aatggctttg gctgcaaaag aaggcggagc agttggtatt cgagcaaata cgacagaaga
    10981 tatcaatgaa atcaagcgaa tcgttgattt accggtaata gggattatca aaagagatta
    11041 cgatgggtca gaagtgttta ttactgcgac agaaaaagaa gtggaagaac ttttggagac
    11101 gcaagcagaa atcattgcac tggatgcaac ttctcgtaaa cgtccgacag aaaaaacaac
    11161 tgctgaactt gtccaaatga ttcacgaaca cggtcggtta gctatggcag atatttcgac
    11221 ttttgaggaa gcgcttcaag cgcaagaaga tggttttgat cttttgtcta cgactttagc
    11281 aggctataca gaatattcac gaaagacgca gacacctgat tttgatttat tgaatcaaat
    11341 catagaacaa gtcgatatcc cagtaattat ggaaggacat actgactctc ctgaacaagt
    11401 agagaaagca tatgaattag gtgctttttc agtcgtagtt ggatctatta tcacccgtcc
    11461 gcaattgatc actaaaagat atgtcgaagc cgcaagtaaa gcaaataact aaaaaacacg
    11521 cacggcacag attcttgaca aaaaagaaaa attgatagat agtttaaagc agattctaag
    11581 aagatgaaaa aagcattact tttcattttg tcttcaagat ctgcttttta acgaatttta
    11641 tagaaagaag gcgaaaccat gtttgaacgg agtccttcag aattgacaga acgtgaaaat
    11701 tacaagttgc tgattggttc agtcatccct agaccagtag cagtagtaac gactaaatca
    11761 gcaaaagata tagtgaatat cgcacccttc agttacttca atatcgtcag ttcgaatcga
    11821 cccatcttat ccttggcgat ccagagaaaa caagatgcag taaaagacac agcaaagaat
    11881 attcttgaga caagagaagc tgttatccat atccttgatg aggacaacgt agaagaagta
    11941 aataaaacag cagccaatct gcctaatgag gaaagcgaac tgtacctgac tgacttgact
    12001 ttaactgatt cagtaatcat ttcagtacca gcattgacag aagcaaaagt tcgttttgaa
    12061 acatccttat atgaacatgt cgagatacgg aataaaaata aagtaacagc tgatttattt
    12121 ttgctagaag tacagaaata tcatttaaat gaaacagtct atgatgataa gacaggaagg
    12181 atcgatccaa gaaaattgaa tgctgtaagc cgtttggcag gaaatgatta tgcagcaata
    12241 ggagaaattt tcacgattgc acgtcccgaa taaaaaggag cactagttat gttaaaaata
    12301 agctataaac catcgacaga ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat
    12361 tttctgcaag ggcagtactt agaagttccg ccattgcaag accattttgt cgttactact
    12421 gttactttag atggtaagga aatagaaatg ccagatcaaa cgattagcgg attgtttaat
    12481 tactttaaca aataaatatt tattccgaaa agtgaaa
//
LOCUS       DACCXM010000080        12288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCXM010000080 DACCXM010000000
VERSION     DACCXM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.082"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(74..631)
                     /gene="frr"
                     /locus_tag="IVY61_001930"
     CDS             complement(74..631)
                     /gene="frr"
                     /locus_tag="IVY61_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP7971039.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(634..1356)
                     /locus_tag="IVY61_001931"
     CDS             complement(634..1356)
                     /locus_tag="IVY61_001931"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP7971040.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1492..2373)
                     /locus_tag="IVY61_001932"
     CDS             complement(1492..2373)
                     /locus_tag="IVY61_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP7971041.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2472..3254)
                     /gene="rpsB"
                     /locus_tag="IVY61_001933"
     CDS             complement(2472..3254)
                     /gene="rpsB"
                     /locus_tag="IVY61_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP7971042.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3613..4092
                     /locus_tag="IVY61_001934"
     CDS             3613..4092
                     /locus_tag="IVY61_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP7971043.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4309..5400
                     /gene="phnW"
                     /locus_tag="IVY61_001935"
     CDS             4309..5400
                     /gene="phnW"
                     /locus_tag="IVY61_001935"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP7971044.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5393..5521
                     /locus_tag="IVY61_001936"
     CDS             5393..5521
                     /locus_tag="IVY61_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7971045.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5525..6070
                     /locus_tag="IVY61_001937"
     CDS             5525..6070
                     /locus_tag="IVY61_001937"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7971046.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6524..8215)
                     /locus_tag="IVY61_001938"
     CDS             complement(6524..8215)
                     /locus_tag="IVY61_001938"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP7971047.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8635..9582)
                     /gene="arcC"
                     /locus_tag="IVY61_001939"
     CDS             complement(8635..9582)
                     /gene="arcC"
                     /locus_tag="IVY61_001939"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP7971048.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9697..10716)
                     /gene="argF"
                     /locus_tag="IVY61_001940"
     CDS             complement(9697..10716)
                     /gene="argF"
                     /locus_tag="IVY61_001940"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP7971049.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10807..12036)
                     /gene="arcA"
                     /locus_tag="IVY61_001941"
     CDS             complement(10807..12036)
                     /gene="arcA"
                     /locus_tag="IVY61_001941"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP7971050.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3380 a         2535 c         2209 g         4164 t
ORIGIN      
        1 atttttaaat aaaagaaata taaataaaaa cagaggaagc ggaatgatcc gcccctctgt
       61 ttttatgatt ttattaaact tccaataatt ctttttcttt atcagcagcg atcttatcta
      121 cttctttgat gctgttatct gtcaatgttt gtacgtcttt ttctaatccg cgcaaatcat
      181 cttcggtaat atcaccattt ttttgttgtt ttttatattc atcgatcgca tcacgacgga
      241 tgttacgaat cgcgattttt gcattttctg cttctttttt aacatcttta gctaattctt
      301 ttctgcgttc ttctgtcagt tgcgggataa ctaagcgaat gacatttcca tcattggttg
      361 gactgattcc gatatcactt gccataatag ctttttcaac atcttgaaga atgctcttgt
      421 caaaaggtgt gatcattaaa acacgtgctt ctggaatttg gattgatgcc atttggttca
      481 atggtgttgg cgcaccgtag tagttcacag taatacggtc caaaaggcta gcgttcgcac
      541 ggcctgcgcg gatttgccca agttcacgtt gaagattttg tgctgctttt ttcatttttt
      601 cttttgcttc tgtcataatt gcatctgcca tttttatttc ccccttacag ttgttccgat
      661 attttcgcct aagattgcac ggcgaatatt tccgtgttca tttaaattga agacaagcag
      721 tggaatatca ttatccatgc ttagtgaact tgcagttgaa tccatgactt gtaatccttt
      781 ggcaataact tccagatgag tcagttcttc aaattttact gcatttgcat ctactttcgg
      841 atcagcagaa taaacaccgt ctacattgtt tttagccatc aggataacat ctgcgccgat
      901 ttctgccgcg cgtaatgctg ctgttgtgtc tgtcgagaag tacgggttgc cggtaccgcc
      961 tgcaaagata acgatccgtc ctttttctaa atgacgttct gctttacggc gaatatatgg
     1021 ctctgcgatt tgacgcattt caatcgaagt ttgcacgcgt gtcggtacac ccacgttttc
     1081 caacgtatcc tgtaaagcta aggcattcat aactgtagct aacataccca tgtaatcagc
     1141 ttgagcacgt tccatgccca tttgtgctcc gatttgtccg cgccagatat ttccgccgcc
     1201 cacaacgatt gccatttcaa cgcccagctc gtgaacttct ttgatttctt ggatgatttc
     1261 tttgattact ggtggtttga ttccaaatcc tgcgtcccca gctaaagctt ctccactcaa
     1321 ttttaagact acacgctgat atttcggtgt gaccatgttc attcctccaa tattttctat
     1381 tgccatttta ccatacttag atgatttcga cactctaaga ctggtttttc ttgttttgct
     1441 taaaaaaagg gagcgcacaa accgtgcgtt cccgtctgtt attcaacaga attatttttt
     1501 cacttggctc ataacttctt ctacaaagtt atcttcgcgt ttttcgatac cttcgccaac
     1561 ttcgaaacgt acgaatgttt taacagttgc gccttttgat gcaacgtatt tttcaacagt
     1621 catatcagga tctttaacga atggttggtc caccaaagcg atttctgctt tgaatttctt
     1681 caagcgtcct tctaccattt tttcaacgat attcgctggt ttgccttcgt tcaatgcttg
     1741 ttctgtaaga acagtttttt catgttctaa ttcagcttct ggaatttgtg attcgtttac
     1801 ataacgagga ttgatagctg ctacgtgcat agctacgtct cttgcaactg tttcgtcagt
     1861 tgttccatct agaacagtca atacggcaat acgtccgccc atatgcaagt atccaccaaa
     1921 tgcagcattg tcgtcttttt ctacaacttc aaagcgacgg aagctgattt tttctccgat
     1981 aacttgagtt gcttcgatca agtcagattc aattgtacct ttatctgttt tgattttcat
     2041 tgcagcttcc atgtctgctg gtttgttttc agcaacaagt tctgcaattt cttttactaa
     2101 atcttggaac atttcgtttt tagaaacaaa gtctgtttct gagtttactt caacgattgc
     2161 tgcagtgtta cctttcacag caactgaagc caatccttca gctgcaatgc ggtcattttt
     2221 cttcgcagcc tttgccattc ctttttcacg taaaagatca actgcttttt ccatgtcgcc
     2281 ttctacttca actaatgctt ttttcgcatc catcattcct acgccagtca tttcgcgtag
     2341 ttcttttacc attttagctg taacgtctgc catgtttaac tcctcctgtg tgtttttctt
     2401 tttaaaaaaa gctgcttcaa aggagagtga ggcttgtcgc ccagcctttg aaacagctcc
     2461 atcattcaaa attattctgc tgaagaattg tcgccttcaa caacatctac gatttcttcg
     2521 attgatgttg ctgtgttttc ttctgtgaaa tcttcttcaa ctacttgatc ttcaccttgg
     2581 tttccttcga tgaaagcatc agccattttt gaagtaatca atttaacggc acgaattgca
     2641 tcgtcgtttg aagggattac tacgtcgatt tcatctggat cgcagtttgt atcaaccata
     2701 gctacgattg ggatatttaa tttgtgtgct tcttgaacag caatacgttc tttacgtggg
     2761 tcaacgatgt acattacatc tgggattctt ggcatatcag cgataccgcc taagaatttt
     2821 tcaagacgtt cacgttcttt gttcaaacca gcaacttctt ttttaggtag aacttcaaaa
     2881 gttccatctt cttccattgc attgatttgt ttcaaacggc tgatacgttt ttggatagta
     2941 tcccagtttg tcaatgttcc gcctaaccaa cgatggttaa cgaagtattg tcctgcacga
     3001 gttgcttctt ctttgattgc ttcttgtgct tgtttttttg tacctacgaa taatgctacg
     3061 ccgccttctt cagcaacgct cttcatgtaa tcataagctg catctactaa tttaactgtt
     3121 ttttgcaagt caatgatgta gattccgttt ctttctgtga agatatattt cttcattttt
     3181 ggattccagc gacgagtttg gtgaccaaag tgtacgccgg cttctagtaa ttgtttcata
     3241 gaaattactg ccatgtttgt ttcctccgat ttggttttat ttttcctctt cttatcttct
     3301 accttgccgg caaacttttt caagcaccta ccgacaatcc aataagaatg tggatttgct
     3361 gatgcataaa gcaccctcag ctattataca gacaaaagat ccgtatcgca agagcaaata
     3421 tacttttctg ctcaacattc aatagtttac ctgaaagata aaaagaattc aagaaaaaca
     3481 gtagatttct ctttgtttct ttattatcct ttttccataa aataatttta ggtataaaag
     3541 acgaatatta ttgggcaatc cttctttttt ttgttatgat acatgtgaaa tctttcgtaa
     3601 aggagaaaga aaatgcgcaa agctcaaaga catttgctga ttaaacaagt cattgaagaa
     3661 ttcccgatcc gtacgcaaga agaactatta aacaaacttg ggcattatgg cgttaccgcc
     3721 acgcaggcaa cgatttctcg agatatccgt gatctaaaaa tcgtgaaggc acctgatgaa
     3781 aatggcgttt cccgcttcgt attgtttcgc gaaaaaggaa ctcctgagac taggactgag
     3841 gaagaggatc ggctggttca aatgattgaa gacattgtat tgaaagtcga acgagtccac
     3901 tttttgacga tcgtcaatac cttgccagac aatgctcatt tatttgcatc tgttttagat
     3961 gagatcaaac ctccctctat tgtcagtaca attgctggat tcgatacgat catggtcatc
     4021 tcggaaaatg aggaggacgc aaaacgagta gaagaatttc ttcaccatcc aactgaagta
     4081 tcactttttt aatcagagca cgtattccta atatggaatg cgtgtttttt tattttcaat
     4141 attttcctta ttgaataaac ctttacactc tttacaaaaa tacatcattt tttattcaaa
     4201 tttttcaaca ccttgattga atcacaataa tcatcattta tattggatgt taacaagagc
     4261 agcttcttgc tctttgtgta tttcaaaaaa gaaatgaaag gaaatcatat gtataaatta
     4321 ttaacttctg gaccattgac gacttctgaa acagtgaaaa atgctatgct gatcgatcat
     4381 tgtacttggg atcaagaata caaagaaatt acacaatgga taagaaaaaa attattggat
     4441 cttgctcaag tatcggaagg aatatacaca acggtcttaa tgcaaggtag cagctccttc
     4501 ggggtagaat cggtattaag ttctgtgatc aggccagaag atactgtact gatttgttca
     4561 aacggcgcat atagacaacg aatgatcaac atgtgcgaat gtctgaaact gaactatacc
     4621 gtttatgagg tatcggaaca tcaaatcctc caagctcatg tgattacaca gctgattgaa
     4681 gagaatccgt ctatctccgc actagcaatg gtccatagcg agactacttc cggtattttg
     4741 aataatttgg aagagattgc tgctgtcaca caagaaagag atcttttgtt tatagtggac
     4801 gcgatgtcaa gctttggtgg gataccgatt tgtgtagccg aactagggat tgattttttg
     4861 atttccagct caaacaaatg tattcaaggt gttccaggct tttcttttgt acttgctaat
     4921 aaagcggaac tggaaaaacg aaaaaatgtt agccgaagtt tgtcacttga tctatatgac
     4981 cagtaccgcg tgatggaaaa cgagaatggc aaatggcgtt tcacttcccc tacccatacg
     5041 gttttggctt ttcaacaagc gctgaaggaa ctagaggcag aaggaggaat cgaaactcga
     5101 tattcacgtt atctcgccaa taatcatgtg ctacgacaaa aaatgacaga gatggggttc
     5161 cagcctttca ttcaagaata tcaggggcca ttcatcacct cttttcttta ccctaaggac
     5221 tggcgatttg attttaatca tttttatcag tatttaaagc aaaatggatt tgtgatctat
     5281 cccgggaaaa tcagcgatca tgaatgtttt cgtatcggaa atattggcga gatctatccg
     5341 gaagatattt tggaactgac agcaattatc aaagactata aggagaacgt taatgattaa
     5401 aactgtaatt tttgattggg caggaacaac tgtggacttt ggctgtatgg ctcctgtcca
     5461 cgcttttaga aatgcttttc tagaaaaagg aatccaactg actgacaaag aaatccgcta
     5521 accgatggga aaattaaaat gggatcatat ccaacagctt ctttccctcc cttctgtcaa
     5581 aaaacaatgg atagctattt atggacaact acctcaggaa aaagaggtgg aggaattgta
     5641 tcaacaattt gaacattatc tgtttaaaga tttggtcaag cattctacct tgaaacccga
     5701 cacattggaa acgatcgaac ggttgagaaa cgctgggatc aatattggtt ccacgaatgg
     5761 atttaatgct cctatgatga caatcgtagc agaaaccgca aaaaaagaaa ggtattcacc
     5821 agacttcatg gcaacttttg aagacgtcga agggtatggt cgaccgtttc cttatatgat
     5881 ccataaaaac atgcagtact tcaaaaacaa gtcggttgat gaagtaatca aagtcggaga
     5941 tacggtctca gatatccagg aagggaaaaa tgccggagtg ttgacagtag gtgtaattga
     6001 aggtagttcg ctaatgggga tttcttatga tgagtaccaa aaattagaga tggggaaaaa
     6061 gatcgtttaa aacagtctgt tgctcttaga ttccaacaag ccggcgctga tatttgtgta
     6121 aacaatttaa aagagttaac agaatacatt ttgtacgctg acatgaatcc agaatgactt
     6181 ttatacaaaa taagaggttg tgaaaaagct gtcctgtatc aagtaataaa aacagccaag
     6241 caaaaatagc tcctcatatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
     6301 gaacaccgtt tattcgaaaa aaaaaaggga gctgtgacac gacttttgtc acagtcccct
     6361 tatttgcaaa caaacagtgt ttaagagaca acctctccac taaacggtgt tcattcagct
     6421 gactctcggt atttgctcaa aaaacgataa aatttgaaaa gctattttct cttttgtttc
     6481 tctaatactc ggatcagaac gctgatttca cattctcttt ttcttacatc ttatctggtg
     6541 catgaagtcc tagcaaacga aggtcttctt tcaataatac agtcactgca taaactagag
     6601 ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt cgtatgagca tagtatttgt
     6661 tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac tgacggttcg tatttttccc
     6721 cagcagacaa gaccgtttca gggtattttt gaactaattt aaccacttcc cagctggtat
     6781 cgtcattcaa tgcatagtct gctgtttctg aagggacaaa tcctgctttt tccaacaggc
     6841 tgactgcacg agcatgtgta tattgcacat aaggtccagt ttctccttca aatcgaacaa
     6901 cttcttccag gttaaagtca aatgtattca gtcgatcgtt tttcaaatcg tggaaaatga
     6961 ctgccccaac cccaacttgt ttagctacag catctttgtt ttccaaatca gggtttttct
     7021 cgctgatttg ttctttagca gattggattg cttcatttaa tacttcttct aataagacga
     7081 tctttccttt acgagttgat aattttttac caccttgcgt gatcaaacca aatggaatat
     7141 ggtgcatgtc gtcagaccag tcaaagccca tttccttcaa gacagctttc aactgtttaa
     7201 aatgatagct ttgttcattc cctactacat atagagattg tttgaagtcg taagtacgtt
     7261 tacggtacaa tgcagcagct aagtcacgtg taatgtatag tgtcgcaccg tctgacttct
     7321 taatcaacgc tggattcagg tcataagcag ataggtcaac gatctctgca cctttatcct
     7381 cgttcagcaa atgtttttct tctaaaagtt tgacgatttc atccatcttg tcattgtaga
     7441 aagcttcacc gttcaacgaa tcgaaacgta cttccagcag atcatagatt ttattgaatt
     7501 ctttcatcga ttcatcacgg aaccattgcc ataattgaat agcctcttta tcgccttctt
     7561 ccagacgttt gaaccaagca cgagcttctt catttaattc tggttctgtt tcagctactt
     7621 catggaattg gacgtataaa cgaagcaatt cattgattgg ttctgcttta acagcttctt
     7681 ctgtacccca ttttttgtaa gcgacgatca gttttccaaa ctgtgttccc cagtcaccta
     7741 aatggttgat gcggattggc tgataaccga ttttttccat aatgaaaccg atcgaattac
     7801 cgataactgt cgaacgcaaa tgtcccattg agattggttt ggcaatatta ggagaagaca
     7861 tgtcgattgg tactgttcct tgattcccga tgttgctatc cccataatgt tctttttctt
     7921 tcactactgt ttgcaaaact tttttgctga taagttcttt attcatgaaa aagttcaagt
     7981 aaggtccgac aacttcgatt ttttcaaagt tggcagaatc gattttttct gctaaatctg
     8041 cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa tgaaaaggct ggaaatgcaa
     8101 catctccgtg ttcagctgat tttggatttt ctagtaattg ctcgacttgt tccagtgtta
     8161 aatcatcttt gacagcgtca tagacagctt ttgcgacaag atctttatta ttcatcttta
     8221 ttcctcctaa agtgttccct tgattgttaa acaaaaaaaa tctctagcga cggcatactt
     8281 agtatgccat cactagagac gagatttccc gcggtaccac tccaattgtc ctaaaaaagg
     8341 accagctcca tctgatatcg gtagacgacc gtctgtttaa aacagaattc tccaagtgcg
     8401 cttcgttgta tcttcttcat tcggcttcca ccacttccga attcgctagc caagaatgat
     8461 acaagtactc tcttgttcaa caaatcttag tcttattaaa ctttcttgaa ttatatcaga
     8521 aaaaaaaacg aaaggctagt cgtttcataa aaaaacaatc cagatggcct aaaggagaag
     8581 aaataccacc tggatagcta taaatcattt cgtaaatacg aaagacttaa caaattaatc
     8641 agctacgata atcgttcctt cttcagaagc taaaagattc tcgatatttt ctaaagaagt
     8701 gatgattgcc ttagcgtttg gttttgcttc aacaaatgca atagctgctt ctactttcgg
     8761 aagcatgctt cctggagcga attgcttttc atcaatgtat tgtttcattt ctgaaacagt
     8821 aacagtttct aatttctttt gatctggttt ttggtaattg acatacacat tgtctactcc
     8881 tgtcaatacg atcagcaaat ccgcatcgat gatttcggct aatttttctg aagcaaaatc
     8941 tttatcgatt acggcttctc gtccttctaa tccagtagct gtttctacaa caggaatccc
     9001 accgccacca acagagactg tgataactcc ttgatcgacc agcgtttcga tgacacgtgc
     9061 ttctttaatc gagatgggtt taggggaagc gactactttt ctccatccac gtccagcatc
     9121 ttctttgaat gtcacagtgg agtcagcatt catttgttct ttggcttctt cttccgtgta
     9181 gaatggaccg actggtttac ttggattttt gaatgaagga tcattttcat ccacaataac
     9241 ttgagtgact aaagaaacca catctttatc gatccctttt tccttcagta cttcgcccat
     9301 ggcgttttgt aaccagtaac cgatagatcc ttctgtcatt gcaacacatg tatctaaagg
     9361 cattgccggt gttttctcag aatcagcagc ttgctgctga atcaataggt taccgacttg
     9421 cggaccattt ccgtgggaga tgatcaattc atctccttgt tcgataaatt tcactaaata
     9481 ctttgctgtt tccattaacg cttcttgttg ggcttttgca cttgcatccg tagacaagat
     9541 ggcattgccc cctaaagcga caactacttt tcgtttggcc atttcattcg tcctttcttc
     9601 agttctaccg aattttctag ataacaaaac gagccagcgt tttgtgattt tccctcaatg
     9661 ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa acgcgaggga taaacaagtt
     9721 acctaatgtt gctgccatga tcgctttgat tgagtgcata cgattttctg cttggtcaaa
     9781 ttgacgagca tatttgctgc ggaagacttc atctgttact tccatttcag tgatgccaaa
     9841 gcgttctttc atttgttcac cgtaaacagt gtttgtatca tggaaagctg gcaagcagtg
     9901 caagaagatc aaacgatctg tgttgtgtgt tttttctacc atttccatat tgatttggta
     9961 aggtttcaac aatttgatac gttcttcgaa tttatcttct tcacccattg atacccatac
    10021 atcagaataa aggacgtctg ctccgtctac acctttatca acatcgtcgg taatcatcaa
    10081 ttgtgcaccg gatttttcag caaagccttc tgccattttg acgatttctt cttcaggttg
    10141 taattctttt ggtgcaacga tacgcatatt cgctcctaaa attgcacctg tgactaataa
    10201 agagttagcc atattgtttc gaccatctcc gcagtaagca acagtgatac cttctactgt
    10261 tccaaagttt tcttggatag ttaaaaagtc agcgatcatt tgtgttggat gccattcatc
    10321 agtcaagcca ttccaaactg gaacaccaga gaattctgct agttcctcta ccattttttg
    10381 actgaatcca cggaattcga ttccgtcaaa catacgtcct agtactttag ctgtatcttc
    10441 tgtggattct tttttaccta attggatatc atttgcacct agatattcag gatgtgcacc
    10501 tagatcgatc gctgcagtag taaatgcaga acgagtacgt gtagatgttt tttcaaataa
    10561 aagcgcaata ttttttcctt ctagatagtg atgtgggata ccacgttttt tcaagtcttt
    10621 caagtgttct gaaaaatcga tcaaatattg taattcttct tttgtaaagt ctttttctgc
    10681 taataagctt cttccttgga atactgattc tttcatatta aacattctcc tttagattgt
    10741 ttttatttaa gattaaagag gaaatcccga cggcagctga cagtaagact gccgtcagga
    10801 aacaaattac ttcaagtctt cacggacaag tggttggctc atgcagcgtg gaccaccacg
    10861 acctcgtgac aattcacttg aattgatttc taacactttg atgccatagc tacgaagcaa
    10921 ttcgttcgat acataatttc ggttataagt gactacaaca cctggagcga ttgccaatgt
    10981 gtttgatcca tcattccatt gttctcgagg tgcaacgatc tcatctccat taccagttgg
    11041 gatcagtaca agatcatcta atccaagagc agctttcatc gtttcgtgta agttatctga
    11101 atgagtaatc ttgatatcat caccatctgg ttcgattgta aagacatcca tcttaccgtc
    11161 tttactttga attgctggat ggatagtgaa tttatcgtga tctaccattg taaatacggt
    11221 atccaaatgc atcattgcac ggttgttcgg aattttgatt gccaacactt tttcaaatcc
    11281 tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa gctttcgcat tagttcgttg
    11341 agaaatccca acagctacta ctttatcgct taagatcaat tcgtctccgc cttcgatatg
    11401 atctggattt tcacgatcca accatacttc gattccttta tttgcaaatc ttggatgatg
    11461 tttcaaaata tattctgtaa acatcgattc acgacgacgt gcttcaaaag tcatcttatt
    11521 gactgtcatc ccatttccca ttgatgcgga agggtcgcgt gtaaagtaca agtttggcat
    11581 tgggtccatg taaaatggat agtcatcgtc tgctgacaga tcatatagac tgctagaacg
    11641 tacttcgata tcttttgtac gaaccccagc catgatttta tctaccattt cttgcgtttc
    11701 cattgataga aggaactcat gaagaccctc tcttacagca ttcgatgcaa tatgagattc
    11761 atccaacatt ttattcaaga attgttcttt gacattgcct gcgtcaatcg cttctgccgc
    11821 taatttttcc aagtaaagag tctcgacgcc ttcattttgc aatgtcttcg caaagttgtc
    11881 atgttcttct tgagcaatcg gaagatatgg aatatcatcg aacaacagac gatccataat
    11941 atctggtgtc aaattttcca cttcttgtcc aggccgtttc agtaaaactg tctttaattt
    12001 ccctatttca gagaaaacgt gaataggttt atccatgttg tgttgcctcc ttctttttga
    12061 ttacatgtat aggatatcct ccttttgtaa tcactttcat aattttgtct cattcaaaat
    12121 gagacattcc tcacattaat attcgataat taaataatta aacgtttttc tatttttaag
    12181 cgtaatttat tcatttttta ttctgtatac actttcaaaa aaatatttat tcattgaaaa
    12241 aatttttttg aataaattaa aaaaataaat tatattatcg ttgttagc
//
LOCUS       DACCXM010000081        12256 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCXM010000081 DACCXM010000000
VERSION     DACCXM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.083"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1210)
                     /locus_tag="IVY61_001942"
     CDS             complement(<1..1210)
                     /locus_tag="IVY61_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7971051.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVL"
     gene            1379..2338
                     /locus_tag="IVY61_001943"
     CDS             1379..2338
                     /locus_tag="IVY61_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7971052.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            2603..3082
                     /locus_tag="IVY61_001944"
     CDS             2603..3082
                     /locus_tag="IVY61_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971053.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            3115..5343
                     /locus_tag="IVY61_001945"
     CDS             3115..5343
                     /locus_tag="IVY61_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7971054.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            5472..7115
                     /locus_tag="IVY61_001946"
     CDS             5472..7115
                     /locus_tag="IVY61_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7971055.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(7188..7733)
                     /locus_tag="IVY61_001947"
     CDS             complement(7188..7733)
                     /locus_tag="IVY61_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP7971056.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(7756..8241)
                     /locus_tag="IVY61_001948"
     CDS             complement(7756..8241)
                     /locus_tag="IVY61_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7971057.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(8231..9043)
                     /locus_tag="IVY61_001949"
     CDS             complement(8231..9043)
                     /locus_tag="IVY61_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP7971058.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(9182..10714)
                     /locus_tag="IVY61_001950"
     CDS             complement(9182..10714)
                     /locus_tag="IVY61_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7971059.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(10823..11854)
                     /locus_tag="IVY61_001951"
     CDS             complement(10823..11854)
                     /locus_tag="IVY61_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7971060.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         4052 a         2269 c         2353 g         3582 t
ORIGIN      
        1 ttaatacttt gatgtggttg tttaagcatt ccaatggtcc gtttgaatag ctgtagtgaa
       61 gcgcattttg gatctgtggc agaaactttt taaacgttcg taaagtggtt gtataggttt
      121 ctggcagacc gctaatgtct ctagttaaac aatcctcaaa tagtgactga tctcttttct
      181 ttaccgcata aagaatgtct tggtataact gatagccttg gcgtaagttg ggacaagcag
      241 ctaacagtcg atcggcaatc tcactttcag tcaaataatc tttaaaactg gcacgccaaa
      301 cccgtttttc atagtttaac tttgcttctt ctttttgtag gagtttccaa tagcgcttca
      361 attttttccc taaccctcgt tgagagtggg aatttccttt aagtcgtacc gtggtttctt
      421 tcactcgatg attcaagaaa gttctgccaa tatgttggac gatgtggaaa cgatcgatga
      481 tcaactgagc attcgggaaa agctttttca ccaacgaaac ataaggcgca tacatatcaa
      541 taacaatcaa ttgaacggct tcacgagtcg ctaatgaaaa ccgagaaaaa tagcgttcta
      601 aataaggcaa gcgtcgattt tcgacaatat ccaataatgc atggctttgt ccgtccatca
      661 tgataaaact cattgcactg gcaacacttc gaaccgattt gaattcatca aagcataaga
      721 cctttggcaa agtctgtttg aaaggattca atgggcggta gaatcgcttc atgacacgat
      781 agaccgaaga agtggaaata tttttctgac gagcgatttc cgtcatggaa gtattctctt
      841 ttaagcgagc aatgacagac caacgaactt tttgactaat gaaacagtgt ttttcagcaa
      901 cagaggtatc cgctacgaat gttctttggc aagacttaca tttaaatcgt tgtttcttca
      961 gctccagata agtgcgatac tcagacacat cattcagtaa caaccgaacg gttgtcgtcc
     1021 cccaacgaat gatcctagag gggacgacac aaagacaatg ggaacactct tctggcttgt
     1081 acagaagagt tcctttgtaa atcaagcaaa tttgcccttt gtatttttct ttagttaaac
     1141 aattttcctc gaaatagata tttaggtcta gaatatttag ggtatctttg ataagctgag
     1201 tatgagacat cttgaatcct cctatgaatt ggtagccaaa ttaattctaa aggatttcag
     1261 ggtgtctttt ctatattttt ttatacgccg attgtaaaat taagctagac aactaaaaaa
     1321 gtcatttgtg ctacactcaa aatagttccg accaaagaat tataaggagt gagtacaaat
     1381 gacctatacc catcttacaa cggatgaact tgtaataata gagtcttatt tcaaaatgaa
     1441 tcaatctgtt gctaaaactg cccattgctt gaatcgttca agacaaacga tccataaagt
     1501 atatctgttc ttcaagcaag gaaaatcagc cttagaatat tatcaacagt ataagaaaaa
     1561 caaatcaaac tgtggtagac gtccgcttgt tttacccgag gaacaatcag aatatattca
     1621 aagaaaggtt gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag cgtttcctat
     1681 tagttgttct gctcgtacca tttatcgtat gttcaaaaag gggctatttg attcttctga
     1741 cttaccgatg aaaggcaagc gtaaaccgaa tggacatcaa gaaagacgtg gaaaacaaac
     1801 tttccgccgc tctattcatg aacgtgaaaa ggattatagc caattctcaa atgagtttgg
     1861 tcaccttgaa ggtgacacta tcgtaggtct gaaacataaa agtgctgtaa ttaccttagt
     1921 tgaacgatta tcaaaagtta tcatcacatt gaaaccgtgt ggtagacaag cgattgatat
     1981 tgaaaaaaaa ttaaatcaat ggtttgaatc tgtaccgaaa aacctattca aatccatcac
     2041 ttttgattgt ggaaaggaat tttcaaattg gaaacagatc agtaatgtca atgatattgc
     2101 catttatttc gctgatccag gaacgccgtc tcaaagaggc ctaaacgaga attctaacgg
     2161 attgttacgt agagatggtt tattgaaatc tatggatttc aattcagtag atgaattttt
     2221 tattcaatct gtcgcatcta aacgaaataa tattcctaga aaatcactgg attatcgaac
     2281 acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaact taatttgaca
     2341 attaagaata caaaaaagat gttggtgaag ttcaccaaca tcaaaaagta tagagccaaa
     2401 atattccaac atcatcttgg actttctaca tgttactaat gagcgtgtaa aagaacgaga
     2461 tggtgttttt atttctatca atgaagctct ttttctttgc aatcctcact tcaatcctct
     2521 ataatgtcgc atatagttta gtaaatgagt aaactactaa tttacttatt tactaaaaat
     2581 aaaaggatga ggagttaaaa taatggaaat accaacaaat ttaaaacata aaccagtaat
     2641 cgttgtagaa gactatgata aagttgatgg aagaaatgca ttaaatatgg atgcaaaagg
     2701 gctttcactg ggccttgctc agtggaatga tcgtggaaaa gtcgatatct caggcaaggt
     2761 atggcgacat actggagaaa aatggtcacg acagtcggaa gaattaccaa tcacacgtat
     2821 tttagattta gcgatcttag ctgcgcaagg aagtttatac ttccaagatg cttatcgtta
     2881 tgaaaaattt tatgatccag aaaatccagt cgtagacatc attggattac aagggaaccg
     2941 aatgacggta gaagtagata ccaaaaatcc aaagatcgat gaagacatca ttttgtatta
     3001 cgatacacta caaaaagacg gagaactgat tggggaacga ttccgtattt taaaacgtct
     3061 tttagatgag ctaggatatt aatagacaga gatgatcaaa aggggaagag aagcatggag
     3121 aatttctcac taacagaaaa ccgaaccttt tcacggctag aaaagcaaat gatctggaag
     3181 aaaccgaaaa gccatcggac aagtgcagaa gaatggcgga tcgcctccga aatcaaaggg
     3241 aattggcaag atcctgaaat gaaaatcagt aacgtacttt tggaaggaga tgccgggtcg
     3301 gggaagacac aattagccaa agcactatct gctgatctgc aacttcccta tacaaaagtg
     3361 acctgttttg cagatatgga caaatcagat gttttcggag ccttattacc tgtagtagat
     3421 tctaataagg aacaagacca agaattacta gaagctattt accaaacaga tacacttgaa
     3481 gctgtactga cactgatcca gaaccattac ggcattgatc ctttttctgc taaagaaaaa
     3541 ttagcagagc tagttcaaag gatcgaagct gattatccag atgcaatcca ttacagctat
     3601 tacccttctg aaatcgttcg agcgattgaa aaaggctatt tattagagat tcaagagccg
     3661 actgtcatcc gagatgcctc tgttcttgtg gcacttaact cagcgctcga accaaatggg
     3721 atgttgaatt taccgacagg aatcattcgt cgccatccag attgcgtcat tgtgatcact
     3781 acaaatcgaa attaccaagg aaatcgccca ttaaatgaat ctttaagaga tcggatgcaa
     3841 catgcggaaa aaatggatct tcctgcctta gaagtcatgg cagaacgtgc gatggcgaaa
     3901 actatgatcc aaaaacaaga acttctctta aagatggcag agattatccg tttgttggat
     3961 gaaactgcaa aagctaacgc catcaaaggt gtagcaggaa tgcgttctta tttttattgg
     4021 gcaaatacct ataagcaagg acaagattta ctccagtcga tttatccaaa agttttgtat
     4081 aaattatcca ctgattcaga tgaattggca attcttgaac aagctttgtc tgacagtgga
     4141 ttgctactgg aacttcagga catattgaga aaagaaaaat ggggtcatcc tcaatctgag
     4201 catacaaaag aaagaaccat tagcgccgaa gaagcaaatg aacgaaacat cactgaaaat
     4261 gctgaagaaa ttctgcgctt agatgaaaag aaatcgttgt ctgctgaaga accaaatgag
     4321 ggagaagaga aacaagaaga aacaatagaa aaaaacgaga ttcctcctca aatgcaaaaa
     4381 gaacgcagta gcgatatcga aggaaaaaat caaaaagaac aacaaacagg agatgattct
     4441 caagaaatgt ctgcttctga catggaagca catgataagg aaatcaagaa gcaactgaat
     4501 aaagaagcgc gacaaatcat gaaggaaacg attcatgaaa aagaaggtct catcgttcac
     4561 cgaccagctt tccaaggaaa aacgccggaa gcacgtgagc tacacatgca agtaatgcca
     4621 atagtagaat cacttagccg gcaaatttta gagttactgg ataatgaaca aacagagacg
     4681 taccaaaaag gaaaatacga aggtcagcgg ttcaatgcaa gcaaagtagc atatggtgat
     4741 ttgagaaatt ttgacaaaaa gaacccgcca catgagcaac cttctttagc ggtagctgta
     4801 cggatcgatg aatctgggtc aatgatacgg gatgatcgga tcgaagcggc aaaaaaagca
     4861 gcgattgcca ttgccgaatt ttctaagaaa gtcgatatcc cattgttgat ttacggagat
     4921 acagcagacc gttcgtcaag agaaaagact tctttgtttt cttataaaga atttgaagac
     4981 ggatttcaat ggttaaatga aaaattagtc actatgaaac caaggcaaaa caatcgtgat
     5041 ggtgctgcac ttcgtttagc agcagaaaaa ttaaacagac aatccgcaac aaccaaactg
     5101 ttgcttaata ttagtgacgg tcagccaaaa gcgttgccgg attatacagg caaaaaagca
     5161 aaagaagata tccagtctgt tttgaaagaa tatgaacgtc aaggaatcct atttgtctcc
     5221 gcagctattg gccaagacaa agaagaaatc aagtcgatct acggtgaaag tcgttttgta
     5281 gatattactg acttgaatga atttcccaag caaatgattc aactgattgc ccgctatcta
     5341 tgataaaata aagctcaacg aacgcactta gcagattcgt tgagcttttc gtttctagtt
     5401 accggtaaca aggatacttc taaaatagaa tcggttataa tcaagaggga ccgttaaagg
     5461 agtgaaacga tatgatacaa gaacaaaaat ggtggaaaga agcagtaggc tatcaaatct
     5521 atcctgctag tttcaaagat agcaatggcg atggtcaagg agacatcaat gggatccgtg
     5581 aaaaacttaa ttatttgaaa gatcttggta tcggctttat ctggatcaat ccgatctatc
     5641 aatctccatt tgtcgataat ggttatgacg taagtgacta tcaagatata gaagagagat
     5701 tcggaacgat ggaagatttt gatttattgc taaaagaagc acatgaatta gggatcaaag
     5761 tgattatgga tcttgtcatc aaccatagtt cggatcagca tcaatggttt gaagaatcga
     5821 aaaaatctaa agaaagcccg tatcgcgatt attacatttg ggtcgacggg gtagatggca
     5881 aagaaccgaa taattggaca tcgattttcg gcggttctgc ttgggagtat tctcatgaaa
     5941 ctggacagta ttatctgcat gtttttgcaa aagaacaacc agacttaaat tgggaaagcg
     6001 agaaattgaa agaagaacta ttcaatatga tccgatggtg gttggataaa gggatagatg
     6061 gatttcgttt agatgcgatt tctcatgtaa aaaaagatga atattcagta aaagccacag
     6121 agaacccttt ctcacccttt caaaatgtct caggaatcga agaacatttg accgatttga
     6181 aacatgtttt tgaagagtac gatatcatga ctgtcggaga agcaagtggt gtcactgcag
     6241 aagaaggacc acaatgggta gggaaagacg gttatttcga tatgatattc gaatttgatc
     6301 atatccatat ctggcagcaa gaaaaagaag ggcaattaga tgtccttaaa ttgaaacatg
     6361 cattaagcgc ttggcaaact tcattggatg gtatcggatg gaacgcttta tatatggaaa
     6421 accatgatgt tcctcgtgcc gtttcagtgt ttggagatac tcgtcccgat ttttgggcta
     6481 tgtctgcaaa agcaatcgct atgatgtatt tctttttgca aggtacgcca tttatttatc
     6541 aaggacagga aatcggaatg acgaatatgc cctttgaatc gatcgatcaa gttgatgcag
     6601 tagattcgaa aagactttat aaaaggctgc ttgcggaagg taagacaaga gaagaagcat
     6661 tggatatcat tcgtgaaaca acgagagaca acagcaggac acccatgcaa tggacttcgg
     6721 aacaatatgc tggtttttct acacatgaac cttggctgat cactaatcca aataccaaaa
     6781 cgatcaatgt agaacaacaa gaatatgaac cagagtcggt cctgcaattc tataagaaca
     6841 tgatccgcat tagacaaacc aataaaggtc tgatatacgg ttcttataaa gaatatcttc
     6901 atgaacatcc acaactttat gtctatgaaa gatatttaga agatgaacaa tatcttatta
     6961 tggtaaactt aacagaatca cttgctgact atgaacttcc aaaagaagca gatcagtcct
     7021 ggacattact cttgtcaaat tcctctagcg gagaatttga agctaaaggc atactagcac
     7081 cttatgaagc tcggttatat aaaacaaaca agtaaaactt cctcgttaaa gttcattagt
     7141 tggactttgc gaggaagttt ttcagattgc tctgtcaatt actcatttca ataattcgct
     7201 ctaggaccac cgatatattt aggacgagag cgaagggctg tcacgtgagc ttgtccaagg
     7261 gttttcaaac gaggtctgac aggtacttgg acatgcttga tatgcatacc gattgatgtg
     7321 tcaccgatat cgataccggc ttgtccaact acgtgttcca cttcgacagg atcattgaac
     7381 tggtcaaaag cagcaacaga acaagcacct ccagcatgta gtgcaggaac aacagagaca
     7441 atttcgaaat tttttgcttc tgccactgtt cgttctacta ctaatgctcg gttcaaatgt
     7501 tcacaacctt ggacagctag cgcaatttct ttaggatcca acaactcttt taacgtacgg
     7561 atgatccatt gaccaacttc cgcactggaa ttttttccaa tatgtcctcc aacgacttcg
     7621 cttgtgctgc atcctaaaac aaaaagatca ccttttttca agtgtgcctc agccaatatg
     7681 tcattaacta tttcagtcaa ttggtctttt aattcttttt ctgacaactt cattttctaa
     7741 aaccttcttt ttcaattaat attgcttccg tgcttgaata cgttgaagac ttgcagttat
     7801 aggaaaggca agtcccattc caatcagatt ttggaagatg tttcctggta tagaagccac
     7861 tccagccggc caatcatata aaatccaacc ggcgatacca tagccgatga tcataactaa
     7921 gcttcctaat aaaagcccta atccccacat acgagtagaa gatttgctga aagaaccagc
     7981 aacatatcct tgtaacccgt ggatcactaa tgaaaaaact gcccactgcg gatatcctga
     8041 aatcaaatcg atcaatagac cactggaagc accaacaact aatccacccg ttggaccata
     8101 aagaaaagca gcggtataga tcccggcctc gcataatgtc acaaatccat tcgtagccgg
     8161 tacagggatc aaaattgtta atgaaagggc aactgtcaac gcaatcagca tagcatagat
     8221 cgttaattgc ttagttttca tttctcattc ctcctttttg aataccgcca gaccagacac
     8281 ttccggtatg atcgttcatc agcacgccat tagaaataca atgggcaaca aattttttac
     8341 tttctgctac tgctttggta aaatctgccc ctaacacaag attcgctgtt attgcagaag
     8401 aaaaggcaca accggccccg tttactgttg tagtgtcgat ttttttatga gaaaatacct
     8461 cataatgatc aattgaatag agaagatcca cagcttcatc tccagccatc cttgtgccgc
     8521 ctttgatcac aatgtttttt gttcctgaag cagtgagttc tttagcgaga tggatcattt
     8581 ctgttaacga agagggaaga gcttgaccag aaagtaaagc agcttctgcc aaattaggcg
     8641 taatcatcgt aacaagtgga aaaagttctt cgatgagctg cgaaatatac aattgattga
     8701 tcacagcttc agtttctttg aatgccaaaa cagggtctaa aatgattggg atattttgtt
     8761 ttgtttttat gaagtctcga acaaccatga ttgcttcact tgaattcaat aacccaattt
     8821 tgataccatc taatgaagtc attttatcaa tcgaaactaa ctgtcgttta agcaattcca
     8881 aagatataga ttccagcata aatcgctcat taatatctag tgcccctata gaagtcaagg
     8941 cgcttagtcc gaatagacca aaattctcaa aagtttttaa atctgtttgg attcctccac
     9001 cagcaaatgg atcagaaccg ccaattgtca agattctttt cataacattt tcctccatga
     9061 ataagagtat aacgaaattt tcaaaaaaga aaaacagcca attggcatat ttttatctca
     9121 tccaatttaa aaaaacaaaa agaaaagaaa gaccaaaatg gatctttctt tccttaaata
     9181 gttaggactt ttgtgatttt aattctttct cgatttgaag ccgttgcact ttcattgtag
     9241 ctgttcgcgg gattgcatca aaatcacgga tgatagggac gttcaagcga ggaagttcat
     9301 ggacttgctc ccaccatcga ttccaatcca tttctttatc aggagctaaa gcaataatcg
     9361 gttgtggaga atgattttta tcccttacaa tcactacttc tgccaaaaaa tctaacgaat
     9421 ctaataaaaa gtcttcaata gctaatgtac tattgatcgt ttcgatcaaa tccacttgcc
     9481 gatctttcaa aaataggtgg ccatgcttat ccataaaacc gtaatctccg ctatcccacc
     9541 aatcgccata aacattttcc tggaaccgtg catcttcctt gtaataagtc aaagcccgtc
     9601 ctttagacag taattgtata tgaccatctg tgttttcagg taggacattc ccaactgaat
     9661 ctgtaatccg agcgcttgtc atgtcttcca gtccaacgcc catatctctt gcatcacttg
     9721 ttttcaaact ctctagtgta tgagccttaa gaatcattgg tccgcattca ctttggccat
     9781 atacttgaag aaataccgca tcctgtgcca tcgacgttcg caaaaatgtc ttcattgtct
     9841 ggttgtttat agcatcaaaa gtggagtgat agtatcggat acccgaaaaa gcttctggat
     9901 gctccttagc agtaaaacgc cactgaacaa agttattcgg atgggtctct agtgcaattg
     9961 gccggtgagc ttccaacata tgaacaactt ttgaactttg tgcgtttgct aaaggcatca
    10021 taggaaatcc catggacatc aaagaagaga taccaatatt gaatctggaa tgcactggag
    10081 agatgtgaaa agcgattaat ttcttttccg aaatttttgt gaaaactgtt ttttgccatt
    10141 tcgtccgcca tcccatagaa tgagcagaat gacaaataag tttagggata ccagttgtac
    10201 cagaagtatg ggtcatataa gaaatttcat ttttgtctaa aaatgattga gaaacagaca
    10261 aagcaggttg ttccattaaa taaagaatag gaatctgttt tgattttgtt ccatttgaaa
    10321 tttctctcac tcgctttttt gtctcatcat caaataaaat gaaagggtct tctaaacgat
    10381 cgataaatac ttccattgtt gttgttggta gatgataaga agtcatgaca ggcactgcac
    10441 caaggtaaga cacagaaaca gctagaaggt atgtgtcaaa agcggaactc ttataaataa
    10501 taactttgtc tccggatttc actccaagat gagccaattg ataagcccgt ttcaacacac
    10561 tttcgcagca ttttttgtaa gttgtttcaa gtcctaattc tgggaaagct gaaagtggct
    10621 catcaaagat aatcggggtc tcggaaaagt tagctgcagc ttcttgaaaa ttcgtaaata
    10681 agttcaaagg ggaatacgtt atggtctcca tcattcacac tccattatct tttgttaagt
    10741 aatatacaaa ttcaagataa tcgtaacata atcctttata taataacata gtttattgtg
    10801 aaaaaaagat catacatttg ttttatatgg aagaaacccg aacgaatgcc gctaattcgt
    10861 gataagaaaa atcaaccatc ttttcccaga tatcttgaaa agaatgggat gagaggtatc
    10921 gattaccaca taaataattt accgcgtcag catcatctaa atatagttct gcataataac
    10981 gatatgtttt tctgcatagt tctggcgata cgtttttata tactgtaatt ttgctcaaaa
    11041 tttggcgaat agtccgagga gaaatttgct gatcatgtga ggaacgaaaa acaaaagaat
    11101 tttcgtccgt attttgtgct gttaacttaa aatagttctc cagaaatggc atcgcttctt
    11161 ttggtattgg tactttattc agtctgtttt cttttctatt cagattaaga atctgcttgg
    11221 tcaaagatac atctttacgt ttaagttcta ctacttcatg gatctttaat ccgcaataca
    11281 gaagaatggc agtaattgca aggttcctcg gtctattctt ttcttcaaag ctagcttctc
    11341 tgcttgtttg aaagccttca atcgggataa acaaatgctc aaaatctttg aaaagttctg
    11401 catcgatata tcgcggagct gctttggttg tttccggtaa acttgtaccc aaaagagaaa
    11461 tctcttcttc aatcagtcct ttttgataca aaaatcgtaa aaaatgattt aatgaatatt
    11521 gcttccttga aatcccgtaa tagctatttt caaacaattg ctccgtttct tggttcgctg
    11581 tagtttggaa cgaacgagta tgtcgataga ttttagctaa gaaattctga taatcttgtt
    11641 tttctaaatg gataatcagc tgatcatcca cttcttcaga aaatactttt ctcaaataat
    11701 cagtaaacag atgaaaatct tttgcgtatt catagcgact gcctaatgaa agggaagaac
    11761 gattttcttc taaaaactgt tgaaagacgg aagggagcaa gtgtgtacgt tgctttaact
    11821 tttcttgcag ttgttttcta gttttgctgt ccattacaaa ttccctccaa tagtgttcat
    11881 tttctatatt ttaccgcatt tcatcaaatg aagatagaca gtttgtcttg atcttaggaa
    11941 gcaaattttt aactaagtac tagctaaaag atatgaagta agaagataca tttttagtta
    12001 ttttattagt gcagtcattc ttttgatctt ttaattgatt tatcctcttt ttagctatgt
    12061 ggttacttca tgagcaatga aatggatttg gttcgttcta atggcaggag gctctttctt
    12121 ttatttccga aaaattagaa aacatttttt cagaatcgat tggctctcca tttattcaat
    12181 aatggtttgt ctaacgatat tgttgggata attcttaaag aagtattaga aatagttggt
    12241 acttcattac tttaca
//
LOCUS       DACCXM010000082        12191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCXM010000082 DACCXM010000000
VERSION     DACCXM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.084"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1163
                     /locus_tag="IVY61_001952"
     CDS             <1..1163
                     /locus_tag="IVY61_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7971061.1"
                     /translation="NFIVYLIYYLLMVIMAVIAQDTLHASLSQAGFASGIYIIGTLLA
                     RLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYLVRFLNGFGYGTVSTAT
                     NAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMTDFRFIIWFSIVLVFFV
                     TIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIAFLMGLSYSSVLSFLSS
                     YTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVMYPSYLFLTAGLFLLSV
                     TTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIALSTYFIGLDLGLGVGPY
                     LMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEGELIESLKNEI"
     gene            complement(1311..1676)
                     /locus_tag="IVY61_001953"
     CDS             complement(1311..1676)
                     /locus_tag="IVY61_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP7971062.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(1823..2596)
                     /locus_tag="IVY61_001954"
     CDS             complement(1823..2596)
                     /locus_tag="IVY61_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7971063.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(2770..3630)
                     /locus_tag="IVY61_001955"
     CDS             complement(2770..3630)
                     /locus_tag="IVY61_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP7971064.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(3630..5048)
                     /locus_tag="IVY61_001956"
     CDS             complement(3630..5048)
                     /locus_tag="IVY61_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7971065.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5105..5554)
                     /locus_tag="IVY61_001957"
     CDS             complement(5105..5554)
                     /locus_tag="IVY61_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7971066.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(5999..6262)
                     /locus_tag="IVY61_001958"
     CDS             complement(5999..6262)
                     /locus_tag="IVY61_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP7971067.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6361..7656)
                     /locus_tag="IVY61_001959"
     CDS             complement(6361..7656)
                     /locus_tag="IVY61_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP7971068.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(7653..8924)
                     /locus_tag="IVY61_001960"
     CDS             complement(7653..8924)
                     /locus_tag="IVY61_001960"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7971069.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9089..9958)
                     /locus_tag="IVY61_001961"
     CDS             complement(9089..9958)
                     /locus_tag="IVY61_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP7971070.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(10250..11860)
                     /locus_tag="IVY61_001962"
     CDS             complement(10250..11860)
                     /locus_tag="IVY61_001962"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP7971071.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3377 a         2620 c         2128 g         4066 t
ORIGIN      
        1 tcaattttat tgtctacctg atttattatc tgttgatggt catcatggca gtcattgcgc
       61 aagacacctt acacgccagc ttgagccaag caggttttgc ttctgggatc tatatcatcg
      121 gaacattgct ggcacgctta ttcatgggga aacagttaga actatttggt cggaagttta
      181 ccttaagagg cggcgcgatc ttctatctcc tttcaacatt agcatatctt tatacgccaa
      241 ctatcggggc attgtattta gttcgattct tgaatggatt cggctacggt acagtttcta
      301 cagctacaaa tgctatcgtt actgcatata tccctgcatc taaaaaaggg gaaggcatca
      361 attattatgg attaagtact agtttagctg ccgctgttgg gccattttta ggaatgattt
      421 tgctgaatat gactgatttt cgtttcatta tctggttttc aattgtactg gtcttctttg
      481 ttacgattgc ctgccttgtc tttcctgtaa aaaatattcc attatctgaa gaacaaaaaa
      541 tatctcttcg ctcatggaat atcaatagtt ttattgaaaa gaaagcactt ttcattacag
      601 gaattgcctt tttgatgggt ctttcttatt caagtgtttt atcattttta tcttcttata
      661 caaaagtaat ccacttagtt gctgctgggt catttttctt cgtcgtatat gctttggtca
      721 tcacatttac acgtccattg actggaagga ttttcgatgt caaaggagaa caatacgtga
      781 tgtatcctag ctatttattc ttaacagcag gattattttt actgagtgta acgacgaata
      841 gtgtgacatt gcttatctct ggagcattag tcggcctagg ttatggtacg tttatgtcaa
      901 atggacaggc tgtttgttta aaaatcgtcc gcgaacatcg aatcagtatt gctttatcta
      961 cctactttat tggtttagac ctaggcttag gcgttggtcc ttatttgatg gggatgctaa
     1021 aatctcttac ttcatttaga ggattgtatg tgattgccgg agtcattcca ttgatatgta
     1081 cagcactcta tcttgttaat ggccgaaaac atagtcatga agcagagggc gaattgattg
     1141 aatcattgaa aaatgaaatt tgatcttgat gaatagataa aaaaaaccgt tcagatctga
     1201 aaataatctg agcggttttt tgagtaaaaa tagtttatga ttattcttaa ttaaacatct
     1261 atttattttt aaagctttca tttatcgata agaatagttt aactgttgat ttattccatt
     1321 ttccgttcat ctggtacgat gccttgactt agcaaccact tgtggaagaa ccattctgcc
     1381 acaccgatca atagagcagg aatcaatact ttcatccaaa tatcgactgt atcattcata
     1441 aaatatagat agagccacaa gataactgca gctgaaccag catctgcgat cgtcgctgcg
     1501 atgttcccta ctttacgtaa gatcaacaga tcccctagca catagatcat gactacaata
     1561 attaccccaa caatcgttga atccataaag ctaacaccgt ataaacctgt cagcacgacc
     1621 cacagaatcg cccagatcat gattgccttc actaccaacg ccttcacatg tttcattttc
     1681 tgcactcctt tctcctggat aaaaaagtaa gattatctaa caagataacc ttttcattat
     1741 tataccatcg gctgagagaa gcgagcaaag ataaggtcct atctaaaaat gaggaagcca
     1801 cttagaacat tcacgtaata atttatccgt acagacattc gatatggtgt tcctttactt
     1861 tcgcatactg ttcttttgtc agttctttac ttgtcaccag ataatcgatt tcatgaaaag
     1921 caatggcttg atgcatgaat ttatgtgcaa ttttttgctg tgtaactaaa acggcaagcc
     1981 ggtctgattg ttttctaaaa gcttttttga ccattgcctc tccttcatga gtttctgtta
     2041 ctccttggct gaaatctagc ccgcgacaac tcattatcgc taaatccgca taatatgaca
     2101 atgcatcgtt ataggctttt gaaccgttga ttgttgctcg tttcggaaca attgcccctc
     2161 ccaatacaga tacttttgcc gttgtatatt cactcaacat agcagctgtg acgatgccat
     2221 tcgtaatgat ctgcaaacca tcaaatcttg ctaggtttct gactaagtgt aaagaggttg
     2281 tactggaatc caagaataag accatatcat ctcgaatcaa agaagaagct gtatctgcaa
     2341 tcttacgttt ttctgtttct tcttctaatt tggtgatctt catcaggtaa ggcacgtcaa
     2401 tttttcgatt gtcaataagc atggcgccac cgcgaactct acggatcaat ccactttttt
     2461 ccagaaaaac aaggtcacgt ttaatcgtgg cttcgctata atttactaac tcgatcaaat
     2521 cggtgactct tgcaaattgg ttgtttttta gataattcac aatctcctgc tgtctattct
     2581 ccacttcatt caacataaaa tcaccccatt tactttatat agtaacagga aaaaagaggt
     2641 tgtgacagaa gtgattggct tcgagaaata agaagaaatt tttcgaaaaa actacttctg
     2701 aacatcgtgt gtggttaaga gggtatgaca agactattgt cataccctct tgaccacaca
     2761 tttttatttt tagtataaac tagccttgcc tactgtgttc aaaatttcca ttttatgttt
     2821 gaccacttct tgtacctttt tattagctgc tggataaatt gcattcggct cgtacatcga
     2881 tggattttca ctcaatattt ctctcaaagc attgaaaaag acgcttttaa gatctgagct
     2941 caaattcact ttggcaactc cgtattttac tgcttcgctg acttctgcgt ccggattacc
     3001 tgaaccgcca tgtaaaacaa atggaagatc gatttgttgg ttcaattctt tcagtaagtc
     3061 gatatttaat tttggagttc tgtttttcgg atacaagcca tgcgctgttc caattgctac
     3121 agcaagtgta tcgattcctg tttctttcac aaaaatcttc gcttgatcag gatctgtata
     3181 aatgattgtt tcagcacctc cttcagcaga accattattc ccaatcgttc caagctcagc
     3241 ctccactgag acatttaccg catgagctaa cgcaaccact tttttcgtaa tctgtacatt
     3301 gtcgtcgaat gatttcaaag acgcatcgat catcacggaa gaatagccgt ttcgaatcgc
     3361 ccgtaagaca tcttctatcg taccgccatg atccaagtgg ataacgatcg gcagtttact
     3421 tgtataagca taggctctta ctgccgcaac aaactcatca cttaaatagg cgatttcatc
     3481 aggatgaatt tctaagatta ctggtgcttc taatctctct gcttcttcga tgattgcttt
     3541 catcatatca tagctgcaaa tattgaaggc tggtactgca aattgattct ctttagccac
     3601 tttcaataac tctttcattg tatataacat tacgcttcca cctcatttgt tagattttca
     3661 tttttcgtcc acttgtcttg ttttttctct ttcactgttt ttttcaatag tccaagcata
     3721 aaggctgtag tgactgtacc tgatgcaatt gccaagacat acataggtaa attgttcata
     3781 acatttggaa ttagcatcac ccaaatccct ccatgaggtg ccattgaagt aactcctact
     3841 gccatggaaa tccctgcagc gactgctgaa cctgtcatca aactaggtat cacgcggatc
     3901 gggtcagata ccgcaaacgg tatagcacct tctgtaatat acgaagcgcc taaaatccag
     3961 catgattgac ctgaagtccg ttcttctttg gtaaatttat gtttgaataa taaagtggct
     4021 aaagcaatac ctaggggagg tgtcattcct gcagccatac aagcagcaat cggtgcattt
     4081 actccagctg acatcaaacc gatcgagaaa gtcgaagtcg atttattgat cggcccaccc
     4141 atatcagctg ccatcatgat cccgataagt aatccgaaca atagcccgga tgtatttccc
     4201 aagctattca gccaatttgt caaagcatcc aacacccatg tgatcggttg ttcaatcaca
     4261 aataccatca aaagcccaat aattaagatc gataaaaagg ggacgacaat cagctgataa
     4321 atagcagaaa attctttttt tacgctaatt ttatttgcca gaaaatcaca gatataccct
     4381 gctagcacgc caccaatcat tccgccaaga aaacctgaac cacctgtaga ggccagtaat
     4441 ccggcaacaa ttcctggggc aaaacctgct tttccagcaa tcgatgtagc aatccccgcg
     4501 cctaacgcag gaaccatcat ggcaaacgct gtatctcctc cgatccttga aaatgcagct
     4561 gctaacacat tatagtctgc tgagttggga tcagccgctg taatcccaaa ggcaaaactc
     4621 agtgcgatga tgattccgcc tgcgatcaca aatggaagca tgtaattcac accattcatg
     4681 aaatgattat agattgtcgg ctttttctca tttgcagctg gttctttttc attagacaca
     4741 acgaatacgc caatatctct caatgcttcg tctattacta cttgcggttc tttgattgct
     4801 cggcttgtag agacacgctt tacttttttc ccggcaaatc ggtccaattc gatttcttta
     4861 tctactgcaa gaatgattgc atctgcctct ttgatttctt gttcagataa ctgattttct
     4921 gtttttacac cttgtgtttc gaattttgct tgatggttta gatttcttgc tgttacttct
     4981 aatttttctg ccgacatata agtatgagca attccagtcg ggcaagcagt tacaccaacg
     5041 attttcatga ttattttcct cctttttctt cactatcgat actcggacaa agactctact
     5101 attttcattc ttgactaatc tgtctgatga tatctgctgg cgtagtggct tcttttattc
     5161 cctttctaaa atctgcattc atcaatcgtc tggagagtgt gctgagaatc tctagatgca
     5221 tatctgctga tttttcagga actacgatgg caaaaatata ttcaattggc atttcatcta
     5281 tcccgctata gtccgttaat ttggctgtat gaaaaaaaca cagtgctggt tttgtcacat
     5341 atttgctttt tgcatgggga atcccaaatc catcttctat tcctgtggag atctcctcct
     5401 ctcgtttgaa tagtgcctta acgacttttt gaacatttct taccttattt gtttttttca
     5461 acacatcccc tattcgctca aataatgatc cttttgaatc tatcgatgga tcgaaaacaa
     5521 tcagttcttc tgcgatcaat tgatcaaacc tcataaaaaa cctctccccc acaaaaaatg
     5581 ttaacgtttt cttggaactg aggtcagtat agcatcgcta gctgtaccgt tcaatattat
     5641 gaatgaacag aggaaaaaga cccatcatcc gcgtgtataa gtcattaaaa tgaacgatct
     5701 agctcacaaa caatcgcaag tctataacaa aacaaaaagc cttccttgaa aggaaagctt
     5761 agcttctaat tttaggtaaa tggtttttta cttcgaaata acccctcttc atttcgttaa
     5821 tataaggtgt ttttatcaag tggataatta aaactatata ctagtttttc attgttttgt
     5881 gcattatgat cctacgactt tttcagcatc aaacgaagtt ttaaattggc aaaaaacata
     5941 aaaaaacaga ctatccctat cagatagtct gctaaacgtt gagttatcag cgtttttatt
     6001 attccgcagc gtttgcgtct ttgagaccgt attttttgtt gaaacggtcc acacgtccgt
     6061 ctgcttgtgt gaatttttga cgtccagtgt agaatggatg tgagtcagaa gttacttcga
     6121 cacggatgac tgggtatgta ttaccgtctt cccattcaac tgtttcttgt gaagtttttg
     6181 ttgaacctga caagaatttg aaaccagttg ttgaatccat gaatactact gggtggtaat
     6241 ctggatggat gttttctttc atgatttttt cgctcctttg ccctgctcca tttgctgaac
     6301 cagagttgat ttgtcgattt acaacattaa catcttaaca tgtttcagtc aaggttgcaa
     6361 ttatcttttc agatttctgg tcggattttt tttaccaaag gagacatctt gaaattcttt
     6421 gaaaaattgc tgattgtttt ttgttttacg caaaaagcgg atcagttgtt ccgtatattc
     6481 taatgaatct ccagtcatat tttttcgaag tttccaaatt tcttctaatt cttcacttgg
     6541 catcaataat tcttcttttc gggtacctga ttttttgata tcgatggctg ggaagatccg
     6601 acgttcagat aattcacgtg aaagttgtaa ttctagattt ccagttcctt tgaattcttc
     6661 atagatcacg tcatccatac ggcttcctgt atcgactaaa gcggtcgcta aaatcgtcaa
     6721 actgccacct tcttcgatgt ttcttgctgc cccgaaaaat ttcttaggtt tatacaatgc
     6781 ggctgggtca atcccgccac tcaatgtacg tccgcttggt ggaacgacta agttataggc
     6841 ccgagcaagt ctagtaatac tatccatcaa gataacgaca tctcgtttat cttcgactaa
     6901 acgcattgcg cgttccaata ctaattcaga aactcgagta tggttttgcg gctgcagatc
     6961 aaatgtagaa gataccacgt cgcctttcac gcttcgttct agatccgtta cttcttccgg
     7021 tcgttcatca atcagtagaa cgattaattc tacttccgga tagttttcgg tgataccatt
     7081 tgcgatttct tttaatacag aagttttccc tgcttttggc ggtgcgacga tcaatccacg
     7141 ctgaccaaaa ccgattggag aaaagatatc gatcattcgg gtagatagtc tttgcggtgt
     7201 tgtttccagt tgaagctgtc tttgcggata aagaggtgtt aaggctggaa aatgcggacg
     7261 ttcttttgct tcttccggat cttttccatt aacgctttcc acgtgcatca aaccataata
     7321 acgttccgat tctttaggtg gacgggcttt tcctgccact ttgtccccat ttctcaaact
     7381 gaatcgtctg atttgtgagg cagaaatata gatatcttct gcacttggtc cataattgat
     7441 tggccgtaaa aaaccgtaac catcttgacc aacgatatcc aatattcctt ccatataata
     7501 aaatccttgt ttctctgctt gtgcacgaat cacagctaga gataattcct ttttattcat
     7561 cttactgtaa taaggaattt taaattcttt ggcataacta taaacttctt ttaacgtttt
     7621 gttctctagt tctgccattg tcaaataatc gctcattcag ctcccacttc ctctatcatt
     7681 tcaattgatg cccctaaggc tgttagtttc tcgataatat gatcataacc gcgtaagata
     7741 taatctacat tgtaaatcgt cgttgtccct tctgccaata gtccagcaat cactaaacaa
     7801 gcacctgccc gaagatcaga agccacgact tctgtgccgt gaagtttatt tggaccattc
     7861 acgatgatca tatttccttc cacggtaata tcagcgccca ttcgtgctag ttccggcaca
     7921 tggttcacac gtttcgcata gatcgtatcg atgatttctg aagtgcctgt agtcatcaat
     7981 agtaacgcag taagtggctg ctgcagatct gtggcaaatc ctggataagg ataggtcatg
     8041 atgtttgcgg gttttaattc tttcgagggg taaacttcga tttcatcttc gctgattttc
     8101 atatggacac caatttcctg caattttgct ataaagcttt ccaaatgttc aaagatcaca
     8161 tttttcactt tgataccatt tcctactgca gctgctaatg ctagataggt tcctgcttcg
     8221 atgcggtcag gaatcatgaa atgccgacag ccatgtaatg tttcgactcc ttcgatacgg
     8281 atcacatctg tacccgctcc tcgaactttt gcccccatat tattcagtaa agttgccaca
     8341 tctatgattt ccggttctcg tgcagcgttt tcaattactg tctgtccttt tgcttttaca
     8401 gcagctaaca tcacattgat cgttgctccg actgaaacca cgtccatgaa aatccgattg
     8461 ccacgcaatc ctttattttc tgtccgtaaa tacatggcac catgttcatt tgtcacttca
     8521 gcacctagag cttcaaatcc tttgacgtgt aggtcaatcg gtctagggcc taaataacag
     8581 ccacctggca ggcctactac cgcttcgcca aatttaccaa gcagtgttcc cataaaataa
     8641 taagaagcac gcaaactatt gatttttccg tgcggcatcg gaatcgatac gacttctcga
     8701 ggatcgattt ctagtatgtt gttggaaaaa tggacagttg cgcccatgat ctctaaaatt
     8761 tcaattaatg aatgaacatc ttgtatgtcc ggcacacctt ccaagatcac tggggaatca
     8821 gctaagattg cagctggaat caatgcgact gcactatttt ttgcaccgct gatcgttact
     8881 tcaccagcta aaggtcggtt tcctttgatt acgatttttt tcatctgttt ccaccttgtt
     8941 tattaatctt ttttcatgga tatgcttgta ttttatcata gataaaaaaa agaaacgagc
     9001 atggtctgcc gttttaagta aaaaagaatg gagagcgact tcaaaagtcg ttctccattc
     9061 ctatatatcg tatctgttat gtggatgctt acgctttgtt agctgaaccg aaccagtcga
     9121 tatgagattt agcatcttca acgattgctg ctttacctgg agctaataat ttacgagggt
     9181 cgaatccttt accttcttta tctttaccag cttcgatata tttacgagta gctgcagcaa
     9241 atgataattg gaattcagtg ttaacattta cttttgagat acccattgag atagcttttt
     9301 cgatttgatc ttgaggaata cctgatccgc catgtaatac taaaggcatg tctggaccta
     9361 cagcatcagc aattgcttgt aagtggtcaa aagctaatcc tttccagttt tctggatatt
     9421 gaccatggat gttaccgata ccgcaagcta agtagtcgat acctgtagca accatttgtt
     9481 tgcattcttc aatatcagct aattcgccgt taccgatgat tccgtcttct tctccgccga
     9541 ttgaaccaac ttcacattca actgagatac cttttgcatg tgctttttca acaacgtctt
     9601 tagctaattt caagttttct tcaaatggta gatgtgaacc atcgaacatt actgaagtat
     9661 agcctaattc gatacattcc aaagcagctt cgtaatcacc atggtctaag tggatagcaa
     9721 ctggaacagt gatgttcatt gcttcaacta aatcagtgat taaatcttta cagattttgt
     9781 aaccacccat gtatttagct gcacccattg atgtttggat aagtactggt gcttgttttg
     9841 cttcagccgc ttcaaggatt gcttgagtcc attccaagtt gtttgtgttg aatccgccga
     9901 cagcgtaacc gcctttacga gcagctttta aaaattcagc tcctgatact actggcataa
     9961 taaaattcct cctaagtttt gtaaaactaa atttgttaag acaattctta accaacgacc
    10021 ctattttacc aaacatttcg gtagttttct actaaaatca atgaaaatga aaattttcaa
    10081 gaagtttttt gcaaaaaata aaatatccaa tagcgcttta atcgaaaaaa gtcttttgct
    10141 ttctatcttt tttcctacaa ataataacaa atcagaaaaa gagaaaagtg acaagaaaat
    10201 tgcttatccc cttctcttgc tacttttctc tttctatcaa gttcaaaaat tattttgttt
    10261 cgtgatctgc taatgatgct ccgacaaatg ctttgattaa tttttgcggg cgatttggac
    10321 gagaaatcaa ttctgggtgg aattggcatc ccacaaagaa ttttttctct ggcagctcta
    10381 cgatttctac taaacggtta tctggtgaga cacctgaaaa gactagccct tgttcttcaa
    10441 acaattgacg atatttgtta ttgaactcat agcggtgacg gtgacgttct tgcacaacat
    10501 cggctccatc atatgcttca gccgtcttcg ttcctttttt gattttacat gggtataatc
    10561 caaggcgtaa agttccaccc aaattctcga tgttttcctg atcagccatt aaatcaataa
    10621 tattgtttgc tgtatctgga ttcgtttcag ctgatgacgc atcttctaat ccaacgacgt
    10681 tacgtgcaaa ttcgacacaa gccatctgca taccaagaca aatacctaaa aacggaacgt
    10741 cattttcgcg agcataacga atcgcttcga ttttcccttc tactccgcga tcaccaaatc
    10801 caccaggaac caagattcca tctgcgtctt tcaaacgttc ttctacattt tctctcgtta
    10861 attcttgtgc tttgatccaa tcgatctcga tgtctgaatt ataggcgaaa ccagaatgtt
    10921 tcaatgcttc aactactgag atatatgcat ctggtaactc gacatattta ccgacaagcg
    10981 caatccgtac ttttttcttc aagttcaaga cacgttcttc taattctttc cattctgtca
    11041 tgtctgcttc tggtgtatct agttttaaat ggtcgcaaac aatttgatcc attccttgtg
    11101 cttgtaaaac taatgggatg gagtaaagtg tatctacatc tggcgactcg ataactgctt
    11161 ctggttcgac atcacaaaat tgtgccaatt tgtttttcac gctttgagaa acaggttgct
    11221 ctgtacgtac caccaaaata ttcggttgga tacctaaacc acgtagctct tttacactgt
    11281 gctgcgttgg tttagttttc atttctccag cagctttcaa gtaaggaatc aatgttgtat
    11341 ggatatacat aacattatct gtaccgacat ctgctttcat ttgacgaagt gcttcaagga
    11401 atggcaaaga ttctatatcc cctactgttc cccctacttc tgtaatgatc acatctgaat
    11461 cagtcatttt agctgctcgc atgatttttt ctttgatctc atttgtaata tgaggaataa
    11521 cttgaacagt tgctcccaag tattcgccct tacgttcttt acgcaaaact tcagaataga
    11581 tttttcctgt cgtcacgttg gagtatttgt tcaaattgat gtcaataaaa cgttcgtagt
    11641 ggcccagatc caaatctgtt tcagcaccat catctgttac gaatacttct ccgtgctgat
    11701 atgggctcat cgtccccgga tccacgttga tgtatggatc aaatttttgg atagttactt
    11761 ttaagccgcg gtttttcaat aagcgtccta aagaagctgc aacaatccct tttccgattg
    11821 atgaaacgac accgccggtc acaaaaatat attttgtcat aagttcaaaa actccttttt
    11881 aatttatgtg caggaaaagt ctcagacaat aaacaaagct ccctattctt caataagaat
    11941 agggagccga aatttctgta aacttctgac cctctaaaag ggtgcccaag gtgtataata
    12001 caagctgact aagggaaagt caagtactca aatgaatact atccgcttac aattttttct
    12061 ttttcgatat cctttgagaa attatttctt aagtccttta tgagtgcaaa aaaaatttaa
    12121 cccacataat aagcttgata taaacatagc taacgttaaa cctaagcaaa tcagaaaatc
    12181 gttactgaaa a
//
LOCUS       DACCXM010000083        11873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCXM010000083 DACCXM010000000
VERSION     DACCXM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.085"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            5..2377
                     /locus_tag="IVY61_001963"
     CDS             5..2377
                     /locus_tag="IVY61_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971072.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="IVY61_001964"
     CDS             2438..3898
                     /locus_tag="IVY61_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP7971073.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="IVY61_001965"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="IVY61_001965"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7971074.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6353..6946
                     /locus_tag="IVY61_001966"
     CDS             6353..6946
                     /locus_tag="IVY61_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP7971075.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="IVY61_001967"
     CDS             6959..7858
                     /locus_tag="IVY61_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7971076.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="IVY61_001968"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="IVY61_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7971077.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="IVY61_001969"
     CDS             8724..8984
                     /locus_tag="IVY61_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971078.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="IVY61_001970"
     CDS             9429..9635
                     /locus_tag="IVY61_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP7971079.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9683..9886
                     /locus_tag="IVY61_001971"
     CDS             9683..9886
                     /locus_tag="IVY61_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971080.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="IVY61_001972"
     CDS             9902..10339
                     /locus_tag="IVY61_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971081.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="IVY61_001973"
     CDS             10332..11039
                     /locus_tag="IVY61_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971082.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11419..11598
                     /locus_tag="IVY61_001974"
     CDS             11419..11598
                     /locus_tag="IVY61_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971083.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT         4445 a         1968 c         2468 g         2992 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgt
//
LOCUS       DACCXM010000084        11771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCXM010000084 DACCXM010000000
VERSION     DACCXM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.086"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(159..536)
                     /locus_tag="IVY61_001975"
     CDS             complement(159..536)
                     /locus_tag="IVY61_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971084.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(836..1717)
                     /gene="galU"
                     /locus_tag="IVY61_001976"
     CDS             complement(836..1717)
                     /gene="galU"
                     /locus_tag="IVY61_001976"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7971085.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1760..2779)
                     /locus_tag="IVY61_001977"
     CDS             complement(1760..2779)
                     /locus_tag="IVY61_001977"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7971086.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3412..4416)
                     /locus_tag="IVY61_001978"
     CDS             complement(3412..4416)
                     /locus_tag="IVY61_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7971087.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4429..5478)
                     /locus_tag="IVY61_001979"
     CDS             complement(4429..5478)
                     /locus_tag="IVY61_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7971088.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5495..6316)
                     /locus_tag="IVY61_001980"
     CDS             complement(5495..6316)
                     /locus_tag="IVY61_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7971089.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6303..7082)
                     /locus_tag="IVY61_001981"
     CDS             complement(6303..7082)
                     /locus_tag="IVY61_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7971090.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7099..7569)
                     /locus_tag="IVY61_001982"
     CDS             complement(7099..7569)
                     /locus_tag="IVY61_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7971091.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7581..8000)
                     /locus_tag="IVY61_001983"
     CDS             complement(7581..8000)
                     /locus_tag="IVY61_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP7971092.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8081..10849)
                     /locus_tag="IVY61_001984"
     CDS             complement(8081..10849)
                     /locus_tag="IVY61_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7971093.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            11049..11273
                     /locus_tag="IVY61_001985"
     CDS             11049..11273
                     /locus_tag="IVY61_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971094.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3414 a         2480 c         1908 g         3969 t
ORIGIN      
        1 cataaaaaaa ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt
       61 atataagtga aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat
      121 ccattatttt atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt
      181 ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct
      241 atccgagact ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat
      301 aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc
      361 ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga
      421 aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc
      481 atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg
      541 aacacacacc tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg
      601 gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa
      661 accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc
      721 cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa
      781 gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac
      841 tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt
      901 ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt
      961 cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa
     1021 gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa
     1081 atattttagg agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca
     1141 tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag
     1201 gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg
     1261 aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca
     1321 tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa
     1381 gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag
     1441 tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca
     1501 attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca
     1561 agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat
     1621 ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta
     1681 gcccagcggc aggaatgact gcttttttga ccttcacttg ctgttcctcc taaacgacga
     1741 ttaactattg atcatctatt taacgaagag aaaattcatt ctccattttt ccgtcacgta
     1801 acatgatttc ttttgctgct tttttgatat cttctccatt atacaaaaca ttatagattg
     1861 tttgagtaat tggcatctca acacctagct gttgagctag ctctactgct gcttttgtcg
     1921 ttgccacacc ctcaacgacc attcccatgt tttctagaac ttcttccagc ttatgtccct
     1981 gtcctaacaa gttaccggca cgccaattgc gagaatgaac acttgtacaa gtaacgatca
     2041 agtcccccac accactcaaa ccgataaatg tcagaggatt ggcacccatc gcgacaccaa
     2101 gacggctgat ttctgccaat cctcgagtca tgattgctgc ttttgcatca tctccgaatc
     2161 ctaatccatg gatagcgccg gcaccaattg caatgatatt tttcagtgct gcaccagttt
     2221 ctacgccaat cacatcatta tttgtataaa tcctgaaata atcattcatg aacagttttt
     2281 gtacataggc tgctgctttt tgatcaatac ttgcagctgt aattgttgtg atatcatgta
     2341 ctgccacttc ctcagcatgg ctaggaccag ataatacaac aattgcctgt cttttttctt
     2401 ctggaatttc ttcagccaaa acttctgaga ttcgtttatg cgtatcttgt tctagtcctt
     2461 tacttgcatg tataatgatt ggcttagtat tcattttacc aataagttcc tgtgcgactg
     2521 aacgaatcgc ttttgttgga acaacaaata aaatagcatc tgcgttatcc actgcttctg
     2581 ccaattcttt atatccgata atcgattctg ggatcgtcaa atcaggtaaa aaatgctgat
     2641 ttgtatgata cttattgatt tcatcaatct gttcaggtac atttccccaa atacggacat
     2701 catgcccgtt ttctgccaat gtttgtgcta aagcagtgcc ccaagatcca ggaccaagaa
     2761 ctgcgatttt ttgtttcatg gatattcctc caaataaata attgataatt tccattatag
     2821 tatacatttc ttaaagttgg gactaatttc cattgttttt aagtgtttgt ttacttcttt
     2881 tatcgttttt acttgaaata atcaatgata tgttacaata gtctacattt tatttttaca
     2941 ctcgttatgc tttgcttgtt cctaagagga agcagttttg acaatgttac taccagcatt
     3001 actattagga ggttaggaaa cttctcctag tgagtattga tttaaaatac ataagagaca
     3061 gattgaacat ttctaaatct gtcaggtcct gcaacggaca gattgtctct gttgacggtt
     3121 gacataggaa cttttgtttt agtcaacatt ctggatacaa aacgctatgt aaactggcgc
     3181 aactcgttgt acttggactt ttactacgga tccatgcaac aactaaatag tactattgca
     3241 taacaaaaaa tggttggcac ccacatgtcc cattaaacag attataagtt gaactgtaga
     3301 taatgcaaaa aatgtgtttt ataacgtaat tgatgccaat attttaaaaa aataaaggga
     3361 gcagttatcg ctgctctctt tattttttat attcttttta tccagtctat cctaataatc
     3421 aaactgacga tagtagcggc gtatctctaa tggatggcag ttcaactgct ccacatgagc
     3481 atcgatgcgt tgtgtcacta catgcatcaa taagaaagac aaaacacgtc cgcgatattt
     3541 ttcactgata ccttctactg gatagtcttt tgtgtctatc aatgtataat tgctgcaaat
     3601 ttttggcaaa agtttagcca cacgttcaga taacactctc tgttcatctt cgcctaaaaa
     3661 taatgtgact ggggttgttt cttctacgat ctctaacgtc ccgtgaagaa actcggcagc
     3721 atggatagat ttcgatggaa gccagctttg ttcttcccag taacacatgg catatgaata
     3781 aacagctccc cattgattcc ctgctccaat aaaataatgc atactatcac gtcgatgttt
     3841 ttctgcaaat gataagccaa atgcatctgc ttttttctct gcttccacta accctgttgg
     3901 taaataacgt tcgagctgtt catagtattc tgaataagct tcgaattctc cattcaaata
     3961 catcaatcga tctgctgcca taaaaaattt gatttgttct gttccaggag ctggataagt
     4021 gaccacaaaa tcgcatagct gagacaactt actgttcgct ttatcgataa atccaatcag
     4081 tgtcgctcct actttcttga tttgttctac tgctttcact acttcttgag ttgtaccggt
     4141 aacagaggac aaaataacga tagaatcttt tgttaatctt ttgtttccag tagtgtaata
     4201 ttctgcagca tgctgtacaa agacaggcaa gttgcttttc ccattcatat aagtgacagc
     4261 ttgcatcgag gaagcatacg ttccaccgat tcctagataa tagacagcgt caaatttttc
     4321 cgcataaagt ttatcgatga tttcttctac ttgcggacgt aaagctagtg cgccttttat
     4381 tgctttgatt tgttcttctt cattgaattt cagcatgatt tccacctctt ataatttact
     4441 tttcaagact gtatcaaagt cttcaaaaat ctttttgttc aagtcaatac ctttagctac
     4501 tgcggtttta taagcgaata gttgaaacgg aacgacaagc gctaatacag acagatattc
     4561 atcacattct attgctaatc caagtgtttt ctcgtttctt gctttttttg tggtaatggt
     4621 gagagtctct ccaacgtacc gactcatata tctttgcaac ttttgcgaac gttccttatt
     4681 tacactttcc ccttcaataa agaatagcaa atgcgaagca tctgcctcca ggtatgggcc
     4741 gtgcatataa gcttctaact caaatccttg tgacggaacg cggattgttt cagtcaattt
     4801 cgtctctgct tctttagctg ttccccagtt tggaccatat ccaatggtga cgaaacgctg
     4861 tgctaaacga aataaacttt gatatccatc aaaaaattct tccgtttttt gaataatcgc
     4921 tggtaaatgg ttgattattt tgcgcaactg ttgcatgtag tcttgttcta tttttttgct
     4981 gattttattt ttacttattc caattcctat ccctaagagt agtaactgta aaacagtcgc
     5041 agaatagcct tttgtaacga agcctactga ttctaccccg gcacctaaat caataatttg
     5101 tgcagcttct aaagcaagtg gactttgcac gtcatttgtg atagcaacaa caggtaaccc
     5161 ttgtttttgt atcattttta ctgcttcaac agtagaagca cttttcccgc tttgtgacac
     5221 agcaatcaca gtatcgattg aaggagaaag ttttccataa ttgaaaaaat aaaaaggttc
     5281 ttgaatatcg acagtaagat caccataaga ttccaatgct ggcttcgctg ctaaacaagc
     5341 gttataagat gaacccgttg ctaagatcag taaattcact gtttttttca tatgttctct
     5401 tgtcgaaaaa tcattctttt tcaagatcgt acttagtact tccttttctt catagatata
     5461 atcttgcatc gtaaacattt tctctgctcc ttttttaaaa aatccctatc cacgaaccaa
     5521 aaattccaaa gaaagctagt ccgatcaaga tcgttaatgg tttcacttct ttcttcaata
     5581 gataaaacac tatgccaaat gctccaagac ataagatatt tggaacgata tcatcaaaaa
     5641 tactttgaac agtcacagca ttttctcctg aaccgatttt taaaggaata ttgatggtga
     5701 tcatcgtggc agtcattgct ccaacgacca tcagtccaat aatagacgcg cctaatgtca
     5761 aactttccat cataccgttt gcctggatct ttttcaaaaa acccgttcct aagcgatatc
     5821 cccaacctgt tgctagatag cgaaacagaa tgtgcggaat attgaaaatc aaaatgaaca
     5881 agatagggcc taaaatattt ccttgcatgg ctaaagaggt acccacacct gtggcaatca
     5941 atcgcaaagt tccccaaaag aaagaatctc ctaaccctgc aagaggtccc atcaaagatg
     6001 ttttgatact atcgatcgca gtcacatcaa attcaggatc atccttattt tcttcctcca
     6061 ttgcagccgt gattcctaaa atcagtgtaa cgatatgtgg ggtcgtgtta aaaaactcta
     6121 gatgccgttt caaagctgct gccatttctt ctttcgactg atataacttt ttcaaaatcg
     6181 gaatcattgc ataaacataa gccatattca tctgacgttc ataattccaa gaaaactcca
     6241 tttgaaaaga acgccaaaat acgtgattta gctctttttt cgttattttg ctgtttttga
     6301 tctcagaatt cttcatcttc ttctacctcc cctgcagttg tttgaagtgg tagttgacgg
     6361 acattcataa tattgactac cacaactgct aaaatcgcac caaaaatagc tactccggtc
     6421 acaggaatct ctaagtaaga agctatagca aaacctaaga aaaaatatgg tgcaacttgt
     6481 ttgttgatca gcaaacgagc aagcatcgca aaaccaagag ctggaatcaa ccctgtagct
     6541 actgacaacc ccttttgaac gaagtcagga atcatattta atagatttcc gatcgtattg
     6601 cttcccacca tataagaaac cattaccaca acaccaagca tcaaggaaag accgataccg
     6661 gcaagcaagt gcatatgctc gactccttta taatttcctt cttctgcata attgtctgct
     6721 ttatggttca taattggaat caaaagccct aagtaaatat ttttcaatat caatgtcaac
     6781 gtagcaatcg gcaaacctag taaaagcgcg gtttctgtac cggctccagc tgtaatagca
     6841 aaagccgtcc ctaaaatccc accggtcaca acatcaggtg gaatcgaagc acctactgag
     6901 aacgatccga taaatgctaa ctctaaagtt gcgcccataa tgatcccagt ttttacatca
     6961 cctaacacga ttcctgtaaa cagccccgtc acaatcggac gagaaagcaa cgatgtccct
     7021 agtgcatatt ctgattgtgc gacaaatgca acaattccta ataagactgc ttgtacaatc
     7081 attgaggttc ttcctttctt acaaaacttc tcttacatcc acttttttat ctgctggtac
     7141 ttgacggatt tctacttcta tttgtttgtc aattaatcgt ttcaagcgtt cctcctcttc
     7201 tggtaaaata tttaccgctt ttccgatgct gcgagtacca ggttttgcct tgatccctcc
     7261 taagttgatg gttttgattt ctggatacgc ttctgccagc ttatatgcat cctcgatcga
     7321 ttccactacg atgaataact tatatttatc tgtcaccccg cttttcaacg cggcaatcga
     7381 gtcatcaatt gatttgatca ctagtttgac tccttgtggc ttggctaatt tgatcgttgt
     7441 ctttcgtatc tcattcgtag gtacatcatc attagcaatc aaaatacagt ctgcgcctat
     7501 tgtttgtgtc catgaaaaag ctacttgtcc gtgtaataac cgatggtcga ctcttgtcaa
     7561 tacaatcatt tttcttcctc ctaaaattcg tcttcttcta ttttctgatt gagccgctcg
     7621 ttacaaaact ggatcatttt ttttgaatca gttagcacct gctggatcaa aacagttgtc
     7681 tctacttcag cgtccaaccg agatactagc tcaatcaata gtggcaaatt caatccggca
     7741 acgagataga catgatcccg attgatatat tgtaagaatt cgttgttgac acttccgcca
     7801 aaaagatccg tcagaacaat gatttcttta tctttgtacc tcataaaaag ctgccttact
     7861 gtttccgcta aatcaaaatt ctcatcagta tagcaatcga atacagtaat agaatgattt
     7921 ttcccataga tcagctctaa tgaatctaat atccctgaag caaattttcc atgggaagct
     7981 aagataattt ctctatccac tttttcatct cctcttttca cttacacata ctaaaaaagc
     8041 aagttgcatg ccaaaattag tggcaacaac ttgcttatgt tcaaaattct tcttctatcg
     8101 agagaaactc tgtatcttca ctcaaaatat catagatata ggcaatctca gaattgggga
     8161 tcatgacact ataatgctct tcaatgacac taaacgcaga tttaatagca gataaccctt
     8221 ctttttgaca ctgcttccat ttctctaatc cttgataggt ttcgattggc atttgtcgga
     8281 tcaatcgttc aatgagacag ctcacatgga catacaacgc taattttttg ggattgctga
     8341 tgcttcgatt caactgcacc tctaattctc tcataaaatg atcgatttct cccatcacct
     8401 tctctgtatc tagaatagtc acggaatcca gtactttttc caatgaaaaa ttacgaatga
     8461 tgcgctgatt gaatatttcc ttctctgaag cattcatagt agtgtccaac cattcagcta
     8521 gtacagacat cttttccccc gagatgaggt cttctaatga aagatagtcg atattggtga
     8581 tatcaggatc tgtggtgcca atcattccta cgacttcgta tatggaaaat aatgggtcac
     8641 tgtttttctt atcttttaat tgctgatatt catatgggat gattttcaac tgacaagccg
     8701 taggtaagct tttttctagc agttcactta agtggaccgc cgtaccgatt ccagtgaagc
     8761 aagtagtaat gagtgctttg ggtttgtttt cctctggata gagcagtcgc cattctggct
     8821 ggatctgtcg aacagtctct tctgctatct cttctaatga tacatttttt tgcaggcatt
     8881 cccctactgt gattgctaaa ggtgtcgtca cattattcaa aatcaaaagc ggtacggata
     8941 actgtttttt aaaaaactga tggatttctt tcagagatcc catgtctact aatatgatca
     9001 atccatttga aacatcgctg ttttcgcaat aatcaaggat ttctgaagca atctgctgtg
     9061 gtgtgacatc tagtggcata tcaaatgatt caaatagatc ctcttgtaat aaacgattta
     9121 ctacatttgc catactgcta gcagttgcat aaccgtgagc agcaatcatc gcttttggca
     9181 gtcgtttttc ttttgaccat ttcgcttttt ttagatagat cgtcagtaaa atccgatcca
     9241 tacttgtgtc ttcaatatct aatttgatct tacttaattc cagaattcgt tggacatagt
     9301 gataactaga tggataagac tgagaaactt gttgttctaa ttctttgatt agttggataa
     9361 tatcatgttc ttccggtaac catttgatag tgcttcgctg atagagatag taactcaagg
     9421 catagacact gtttccatcg aatttgattt gataggcagt ttccatttgt cgaaaggtct
     9481 ctcggataga ttgtgtccaa taaaccagca gctcgtgttt ttcttgtcta ctggtctcaa
     9541 acagcaaaaa atcaaataat tgaatcactt cttgctttaa ttttccttcg caagaatata
     9601 gcagacagtt atttttcttg aaatcaatta ggatttgctc aaaactttta acgattcgct
     9661 gctgttcggg ctgttgcttc tccattaatt gctctaaggt ctggtcttgc gtaatataga
     9721 ttccttgctg tgctgtattt ttagtaatag gctgcgcaag tagttctttt tgcaaatgat
     9781 ggatcgtgac atggatttca ggctgttcac gctgttcagc atatgctttt gctgctgaaa
     9841 ctttgacgat atttttcagc tctcctatgt ttccggataa tttctgactt gttaaaagtg
     9901 acaaagctgg tccactgact gacaaagggc tccccatttt tctttgttct tcgagaaaaa
     9961 aagagtaaat cagttctagc cgttcattac gactacgctg attaagtgca ggtagttcga
    10021 ttcggatagg tattcttcga atgaaagttg ttaagaaatg actagtaatc tcttcagtcg
    10081 tagcaaaaaa gagacgtgtt ttgatccggc gtgacaaatt aggttctccc atacgataaa
    10141 tcacaccttg atcaagatag gtgaacagtt tttcctgacc ttctggtgtt aagcgatgta
    10201 cctcgtctaa aaacaagatc ccaccatctg ctgcttcaaa agcccctttt ttattttctt
    10261 ctgcaccagt gtacgctccc ttaacatgtc caaacaaatt gctagtcaaa agttcaggat
    10321 tatctgcata ttgtgcacaa ttaaaagtga tgaatggtgc agaatcttct agcagatcat
    10381 gaagcagaca atactgatag accaaattaa ctaaataact tttacctgtg ccactttcgc
    10441 ctgtaatcaa aagtggtaat ccgccatctg gatagtaaag agctgttttg atttgttcga
    10501 tactttcttt caaactggcg tcgtatccga tcataaaaga gaataagtct tttggttgcg
    10561 cgaaaaaagg ctgttcctct ttgagatcat cgatagaaaa ataaaaattc gtgcgtaact
    10621 taaaaaattg tttttcaaaa gcttctttgt ggaaataaat cactggtcga gaattgattt
    10681 tgactagtag ttgctcatca tttaactggt tcaaataatg actgactgtg tttcttttta
    10741 ccccaaaatg atcagctaac ctttgtgctg taaagacctc gttcatatgt tctaaatcga
    10801 aaaaggctgt ctggttctct aagtaacata aaatttcctt cttcaacaac ttattcaccc
    10861 ctcctccatc atagaaaacg gattcatttt gtcaataaaa tctttaataa agaattgaaa
    10921 aatatatctt attatagaca gcagcctact tatcccttat aatttttatt ctaaaaaacg
    10981 tacagttcct tccagttttt atactggaat ttcaacatat gaaagaataa ggaggctatg
    11041 actattcaat gaaatcggat aaatataaaa acaggaaaca tacttcctgg aaaacttttc
    11101 gagaaatcat cggatcttat ctcgctgtag ttgtattttt gcctttgctt tatatggcta
    11161 tgacagaaca aactcgtttt attggcgtag atacgcaata tatagccgaa acaggatttt
    11221 tattcatctt tatcttgatt tttgcgttct ataaatcctt ccatacaaaa taagattcac
    11281 agatttcaac tttcctgtct gtttttagct attacaaatc cttatatgtg gtataacaac
    11341 aatttattgg cataatgagg aaaaacgagt tatgatgctt tcagagagta aagaggtact
    11401 gttgtcacca tcctttttat tgattacgta caccactctt agttttttcc cattgattac
    11461 gaaaacaagg tagaaacatt gatgttccta ccttgttttc tttttttatt ttcagatcaa
    11521 aatctcaagt ggcttttgac ttgatgtcgg gaggttgtct tcgttccatc aaacctgata
    11581 gaatctgcgc caaaccatag ttttctccac tattatttat tcctctaaga tagtacccac
    11641 aataaacgaa taaatatttt tctcattttc atactccttc tttgaaaatc aattagattt
    11701 ctctcaaccc acttttttac aaaaattacg tagcaaaaaa gattgcaaga acaaattaaa
    11761 aaagaagtaa a
//
LOCUS       DACCXM010000085        11745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCXM010000085 DACCXM010000000
VERSION     DACCXM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.087"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..132)
                     /locus_tag="IVY61_001986"
     CDS             complement(<1..132)
                     /locus_tag="IVY61_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7971095.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..841
                     /locus_tag="IVY61_001987"
     CDS             542..841
                     /locus_tag="IVY61_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7971096.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            914..2275
                     /locus_tag="IVY61_001988"
     CDS             914..2275
                     /locus_tag="IVY61_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7971097.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            2297..2557
                     /locus_tag="IVY61_001989"
     CDS             2297..2557
                     /locus_tag="IVY61_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971098.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2571..3323
                     /locus_tag="IVY61_001990"
     CDS             2571..3323
                     /locus_tag="IVY61_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP7971099.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3417..4022)
                     /locus_tag="IVY61_001991"
     CDS             complement(3417..4022)
                     /locus_tag="IVY61_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7971100.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(4075..5049)
                     /locus_tag="IVY61_001992"
     CDS             complement(4075..5049)
                     /locus_tag="IVY61_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP7971101.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5427..6206)
                     /locus_tag="IVY61_001993"
     CDS             complement(5427..6206)
                     /locus_tag="IVY61_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7971102.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(6273..7544)
                     /gene="celB"
                     /locus_tag="IVY61_001994"
     CDS             complement(6273..7544)
                     /gene="celB"
                     /locus_tag="IVY61_001994"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7971103.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7697..9094
                     /locus_tag="IVY61_001995"
     CDS             7697..9094
                     /locus_tag="IVY61_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7971104.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            9175..9615
                     /locus_tag="IVY61_001996"
     CDS             9175..9615
                     /locus_tag="IVY61_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7971105.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9763..10089
                     /locus_tag="IVY61_001997"
     CDS             9763..10089
                     /locus_tag="IVY61_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7971106.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10090..10839
                     /locus_tag="IVY61_001998"
     CDS             10090..10839
                     /locus_tag="IVY61_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971107.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10919..11374)
                     /locus_tag="IVY61_001999"
     CDS             complement(10919..11374)
                     /locus_tag="IVY61_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7971108.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3747 a         2055 c         2401 g         3542 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaagaaaa
      541 aatgaaaaat cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc
      601 tagtaagatc caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga
      661 ttacaaatcg attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc
      721 tgatgaggaa gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc
      781 atttttaact ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta
      841 aaggaggatt gttggataaa aaagttggtt catagtttta tttactatat ttagaaagaa
      901 agaaggttta tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc
      961 agccattttt gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa
     1021 aaaagcattc atgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
     1081 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
     1141 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
     1201 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
     1261 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
     1321 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
     1381 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
     1441 tccattagta acagtgactc attttatgac atctgccgct gtcttgttgc tgcctttcaa
     1501 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
     1561 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
     1621 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
     1681 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
     1741 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
     1801 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
     1861 gtttattggt tatgccatta ttttgccagg aaaccaagta ttgccattag cagggattat
     1921 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
     1981 gatcttggga atcatcacta tgcctattta tttgtacggg gccacttacc ttgcgccaat
     2041 actcagtgat ttagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     2101 cacatggtca tccatagaag ggccggagtt cagggtttta tttgcggagg catttaatag
     2161 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     2221 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     2281 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     2341 ttgtggtaca cttctgctat ttctatatgg actctttact cagaatatca tcatcaatct
     2401 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     2461 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     2521 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     2581 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     2641 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     2701 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     2761 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     2821 atttaactcg tgaaatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     2881 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     2941 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     3001 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     3061 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     3121 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     3181 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     3241 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     3301 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     3361 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     3421 ttagttaaga ggtggtctgt ccattccctc gcttgcttga tttcgtccat cgtcaactga
     3481 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     3541 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     3601 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     3661 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     3721 tttgaatagc cgacaagcag ccagtcactt aacggtatca gttctttttc gctgatttct
     3781 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     3841 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     3901 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     3961 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     4021 attttgttcc ctcttttctg tttctataga tgcaaatgaa gtagcatgaa gagattattc
     4081 acgtttttta tttgcatcac ttgtatcgaa aggacggaca taagcttcaa tggcttcacg
     4141 acgttcttct aagaatggag gaagagatag tttttctcct gctgattctg ctggttcatc
     4201 ttcaaggaat ccaggaccat cagttgctaa ttcaaacagt acgtgatgag cggctaggaa
     4261 gtactcagat tggaaataga aacgatcgac gaaaccagaa tttggcaatt caatcttatt
     4321 gattacttga atccagtatt ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg
     4381 gatatttcca aatccttcga ttgcttttgg caagtctgtc cgatgttcaa taatgatgct
     4441 tgcaccattt cccccttcac cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc
     4501 tttgaatcct aagatttttt caagaatcaa gcggatatgc tctaattgac tgacacggac
     4561 aaagacaggt ccaagtcctg tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg
     4621 aatgccagct gctacacctt gattgttttc gtcagagatc aattgataat actgattatc
     4681 gaaatcttca aattcgatat attttttgcc gaaacgttca ttgattgctg aatgagtaac
     4741 gtcatatttt tcaaaacgtt ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa
     4801 agaagcacga gagatcgtat ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa
     4861 gaaagtcata tctgttcctg gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc
     4921 gatgtcatct tggttaactg tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa
     4981 gcggtagatt tttgtagcgc tagatgtcat tgctgtgacg tggtgaattc ctctgatttg
     5041 tgtatccatg ataaatttcc tcctgttaac ttacaaaaag taagtagttg atttagacat
     5101 agtatagagg gtgcatctct attttgcaat taatttgtct tctaaagtat caggaaggaa
     5161 tcaagaatgg aaaagaggag ggacggatca ataaaataaa aagacttacg actagctgta
     5221 tcgatttcaa aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa
     5281 gtctttttat gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg
     5341 ttgatttcac gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt
     5401 aaacggcttt ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc
     5461 cgaacatcaa atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag
     5521 acgacttgct gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca
     5581 tattcatcac ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat
     5641 tcttgatgaa gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca
     5701 agatggactg gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac
     5761 cggtgaatat tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga
     5821 ttagggaatt caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc
     5881 atttggagac tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga
     5941 tgattcaaga ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag
     6001 atattgatgg cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact
     6061 tgattaggca aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata
     6121 tttgcgattt cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt
     6181 atgcttccga caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg
     6241 acaagttttt gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta
     6301 tctagtactt tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc
     6361 agcataccac caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc
     6421 catggaacag agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg
     6481 aacgtcgtta cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg
     6541 atcggtagac cgaagatcgc tggttcattt acattgaaaa taccaggtgc taaagctaat
     6601 cgtccaacat cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct
     6661 aaagtcattc cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg
     6721 atgtgtggta attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc
     6781 caaatcgggt ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag
     6841 aattggacaa agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat
     6901 ggtttttgga tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc
     6961 acagcattga ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat
     7021 gattttgtta ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg
     7081 attctgcagt agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt
     7141 gcgccaagac gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt
     7201 gcaaaaggcg taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca
     7261 acgtcataag attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg
     7321 ctcattgttg cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca
     7381 ctgaaaaaag gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag
     7441 gaaagcatga aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct
     7501 aagggcatga ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa
     7561 tttatttgca atcgattaca tacaaagtat aacgccaaaa attttaaatg tctagtcatt
     7621 ttcataaaaa gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa
     7681 gagaggaagt aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc
     7741 tgctagcgga ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg
     7801 ggatcattgg tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac
     7861 ttcgcaagtt tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa
     7921 cacatttcgt acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa
     7981 tcaaaaagct gtttcttttt atcgagatta ttttactgaa ttgctggata atggtattga
     8041 accttttgtg aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg
     8101 gttatcaaga gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt
     8161 tggagatctg gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg
     8221 ctacttgtat caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg
     8281 ctatcatgag aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga
     8341 tggtgagatc ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc
     8401 agaagatgta aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc
     8461 tgcagtgaaa ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat
     8521 gccagagatc catgaaggag acctagaact catccgtgaa aacacgattg acctgctagg
     8581 aatcaattac tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca
     8641 tagccccatg ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc
     8701 atatcgtgga tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga
     8761 aaactatggt aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga
     8821 acgatttgtt aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga
     8881 tcatttgaaa tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat
     8941 gtggacatgt atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat
     9001 cgctgttgat ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa
     9061 aacggtctct gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa
     9121 tgtttgttct aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta
     9181 gatacttatc gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt
     9241 cttgaggcaa tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat
     9301 gaagtaacag gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa
     9361 ttggatgagg ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc
     9421 ctgacagata tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc
     9481 catgaatcga aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta
     9541 gaacgtgcac tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc
     9601 atggttgtaa aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg
     9661 ctttctgttc cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta
     9721 ttctaattac aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga
     9781 atacgcggat acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga
     9841 tatgaaattt ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca
     9901 acactttact ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga
     9961 gaagtgactc atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt
    10021 ttagaagaaa aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag
    10081 ggaaattaaa tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa
    10141 agtaccgaga tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat
    10201 gaattgatgg gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt
    10261 ggttcaatcg atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag
    10321 acggaaacaa acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac
    10381 agagcggcaa gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt
    10441 tttcttttct tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt
    10501 atagcattag ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac
    10561 tttttgcacc atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa
    10621 gagtatcagc gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca
    10681 cttttcccac ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata
    10741 ttttttgtta cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat
    10801 tcttatcgtc aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa
    10861 tattctattt atccggcgta gaaaaaaaga ctgtgacacg cttttgtcac agtctttttt
    10921 atatataata atttaaaaat tcatcatcta aatccgtaga attttctaga ttgcttaccc
    10981 atgtaaaatc gttatagaca gatttcagtt gacttctgcg ccattgtaca gggataaaaa
    11041 gacgcatgct atcttcattg aatccgacta ataatttctt tccgtcagta atgatgggac
    11101 gttgtaacaa agtaaaatgc gagacgatca gatcgacaag ctctgaggtc gtacattggt
    11161 cgatttggtc tttcaaattt ttataatcaa tcgagcgttt agctaataga tcgtcaaatc
    11221 cgttttctgt taatgacaat aaataaaata tctctttcct tgttaaaggc tgtttaggtg
    11281 tgaaaattcg ttctctgtaa ggcatttgat ggtctttcag ccagtttgcc atcagatggt
    11341 aactatgtgt gcgagaagaa tagatcgtaa gcataaaaaa actccttaaa tagtccatta
    11401 atttgttaga tctgttgaga taaataatag gtaaggagct gtgcataagc attgatcaaa
    11461 acaacccaaa catcattaga aataactttc taaagtctta aacttgcgac gagaagcaac
    11521 tagactaata gaaaaaatgt cttagccatt tggcttcctg tttgtttttt tgcttatgct
    11581 gcagctcctc gggaaagggt tctaacaatt tgaagaaggt tgatgattga tctagtttaa
    11641 attgtgtgaa atttgaaatc tggtgttttt actagatgaa tcacctaaca actatgatta
    11701 taacttatag aacttctgtt ttgcattcgg ttttgttata gttgt
//
LOCUS       DACCXM010000086        11472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCXM010000086 DACCXM010000000
VERSION     DACCXM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.088"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            276..482
                     /locus_tag="IVY61_002000"
     CDS             276..482
                     /locus_tag="IVY61_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP7971109.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(684..1685)
                     /locus_tag="IVY61_002001"
     CDS             complement(684..1685)
                     /locus_tag="IVY61_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7971110.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1690..3603)
                     /locus_tag="IVY61_002002"
     CDS             complement(1690..3603)
                     /locus_tag="IVY61_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7971111.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3771..4277)
                     /locus_tag="IVY61_002003"
     CDS             complement(3771..4277)
                     /locus_tag="IVY61_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7971112.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4437..4877
                     /locus_tag="IVY61_002004"
     CDS             4437..4877
                     /locus_tag="IVY61_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7971113.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4903..6060)
                     /locus_tag="IVY61_002005"
     CDS             complement(4903..6060)
                     /locus_tag="IVY61_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971114.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6063..6419)
                     /locus_tag="IVY61_002006"
     CDS             complement(6063..6419)
                     /locus_tag="IVY61_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971115.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6717..7691)
                     /gene="sgrA"
                     /locus_tag="IVY61_002007"
     CDS             complement(6717..7691)
                     /gene="sgrA"
                     /locus_tag="IVY61_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP7971116.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7887..8726)
                     /locus_tag="IVY61_002008"
     CDS             complement(7887..8726)
                     /locus_tag="IVY61_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7971117.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8911..9798
                     /locus_tag="IVY61_002009"
     CDS             8911..9798
                     /locus_tag="IVY61_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP7971118.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10392..11086)
                     /locus_tag="IVY61_002010"
                     /pseudo
     CDS             complement(10392..11086)
                     /locus_tag="IVY61_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(11070..11468)
                     /locus_tag="IVY61_002011"
     CDS             complement(11070..11468)
                     /locus_tag="IVY61_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7971119.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3422 a         2292 c         1718 g         4040 t
ORIGIN      
        1 aataaaaaag tttaggatac atcgaatttt tggggaaatt cttttggggg tatcctaaat
       61 cttttctatt atattcccat attttctatt attggtctag acgtatgatg gtaaactttg
      121 tacctatctg attgctgaat cattaattgc ttatcattag ccatctttgt ttcaagttcg
      181 ctagatgatc attttattca catcctaaca aacttgtcac atttatagat tcttaatcta
      241 tttatcatac aattataaaa aaggaggtgc tgcttatgaa agctatccat atttctttgc
      301 tttttttcgc tagtgcattg attttatttg ctattcatct ttgttcccag ccttatatca
      361 ataacaacgg aatattagtg gaaccagcat tcttttgctt accactcggc tttttgtccc
      421 ttcttgcctc cgcaggcttc ggatttgttg ctttttttaa acgatcaaaa caacaaatct
      481 gacttataaa atttacgtaa aagagatcgt gacaataatc gtgtgatatt cccaaatttc
      541 gaataaaagg tgccccttgc catctctaac tcctcagtca caagttacaa tattcataaa
      601 ccatgaaaag cttgtttatt gaaattgctc cttgtgactt ggagctaagt gacagattac
      661 aacctctttt gcatcataag actttactcc ttgaaaaaca ttactaactt ttgatatgtt
      721 tcttgcgctt gtttattgct gaaatcaaac tgatattcat ggttcaaaac agcatttttc
      781 tccgcataat ctacttttgt gactttcacc cccgctttct caaatacaga agttacttct
      841 tccatttgtt tcgtaaacgt atttgtattt ccatccgtta agaatgtgtt ttgcgtccaa
      901 cgatccgcat gtctgaccaa ggctaaactt ttgaccttct ctgattgttc aaagtcctta
      961 ttatcaaagt aagcccatcc acatttttca taaagaaagc tagagatttt atcacttact
     1021 tgagtaagtg cagaaggatc caacagacca gacaaagaaa taaacttcgt aatctgcttg
     1081 ttctccaaag tcggatcaag atgatcagct tttttaattt cttcattttg aagtgctaac
     1141 aaatattctc cactaatttg tgcacctgcc gaatttccac caatcgaaat attcgaccaa
     1201 tctgcttgat cttttatctc ttttttagct gcttgaatgg cttcgttcaa ttggataact
     1261 ggtgtaggat gttttgcttc cggtgatcgt tcataatcta tatttacgat gatttgctgc
     1321 gtgtctgaac atagatattc caaataatca cgacaatcat ttttatcccc tcctacatat
     1381 gcgcctccat gcatccagaa aagaatcggt aattttttct tttccacttt cggtgtgtag
     1441 atatccatat tattattttt gtactttgaa ggataggata tttctttttt tatatcgatt
     1501 tgttcgattt tttgctggaa atcatctggt cgggcatata cttgttcttt ttcacttgtg
     1561 tcaaattgtt ttctaacaag caagctaatc ggctttggtg tgatttgatt aatcaaaaat
     1621 acagtgacta aaataactac gatgataact ggtactgcaa tcactattcg cttgaaccat
     1681 ttcattcatt taccctctct ttcttcgcca aatccatgtg ataatcgtgc taacagataa
     1741 aaatccacct gaataaatga gaagcgaatg tttcttttct ccagttttcg gcaacattct
     1801 tttttttgca tctgtctttt tttgtacttt cactgtcatc tgaatcggtt gcgtatcatc
     1861 acttgattga tcctctcgag gttcattctt tcccttattg tctgcattct ctgtcggatc
     1921 ttgctttttt ggtggagcta ctgggatttt tttgtaatag aaaataatag ttggtatcgt
     1981 ctcattaaaa atcccttcag tcggtccttc aacatttgct aattcgtatc cttcgaatgt
     2041 cttttgctct gtcatataat tttctccata agctccgtaa agctggattt cgtcctggtc
     2101 tttgagtaga cttttattgt tttcatccac atactttacg tttatctgat catggaacac
     2161 tactttcaaa aagtccgtta catgtttcgt gtaggcagca tggtcaactt cctgtgcctt
     2221 tccatgccct gctccttcta ctaataaaag atctttatac cctcctgata actcatataa
     2281 tttttgcgcg ttagacacag gtactacatc atctgctgtt ccatggataa tcaatttagg
     2341 caatgaatct gacattccaa tcgtaggtag agcagctttt gtatccaaat gcatctcctg
     2401 atcaaaatag tattgtttca aaaggcccat aaataaatct tcatgctctt gatccccaat
     2461 gatgtctaac tgatttttta tggcatccgg tagtgcgggg gctactgtct ggctgtacgt
     2521 aaaacgtagt tgttcatcaa ttgatgcaaa tccgcaattt tcaataattc ctttgacatt
     2581 gacaggaata tctttcgcta atacagacat catcgtagct gctcccatcg aaccaccata
     2641 aagtaaaatc ttttgagatg gccgctctcg aacttcttga tttatccagt aaaggacgtc
     2701 atctgactca tattgtccat aggtcacata atcgcctccg ctattaccag tcgctctgga
     2761 atcaacaaat aataaattat acccggcctt ataaaaatca ttgtattctt ctgtaacgat
     2821 cccgttattc caattccctc gaaatccgcc atggatcatg actgttttat ctgaaccttg
     2881 atcgacataa tatgcatata catccatcat tttgccatct tgtttgttta gtacagtttg
     2941 atgaagttgt tttgtatttg gatagaagcc tgtgttttct gtaggcgttg tcccatcatc
     3001 tgtacctcca agcatcgaca tcaacacact gttttttgca aaagcgatat cgtacaatag
     3061 ataagtagca atcagatgtt tgataatctc tttatctaca ccataatcat taacagcaag
     3121 ctcagtgact aataactcta ttaattttga cttgatgtct tctgttggtc cattaagtat
     3181 ttctgtcatc tgtttaccga agtaattcaa atattcttta ccattgaaat tttcaccttt
     3241 accgatatca tcattttctg tcccattctc tttgtcactt actttagcag aagaggattc
     3301 gtcgtttgac atttttgtag ctgttgcttc ttctgtttgt tggttatctg tggtagcggt
     3361 cgtttcttga tctgtgctag taacagatgt ctcaaccgtt gtttcagtat ttgtttctgt
     3421 ttgtgaagta ctcgatttcg tttcatttcc agcatcagta gtttctgtgg tttctacctc
     3481 ccgagattcc gattcggaag aaatgctctc tccatatact gtccattgcg ggacgcttgt
     3541 acttacagca aataagatga ccaacgctcc ctttaatact tgttttgttc tcaagctatt
     3601 cactcctttt tatgtttcta ggagaattat atgtaaacgc aaacattttt tatacaaaaa
     3661 ataaatggtt tatcccataa aaatagaaca ggattatttt ctcattttac gtaataaagc
     3721 agaatggata aaccagtatc cattctgctt tttttaaacg aagaaacaac ttattgctcc
     3781 aaaaatgtca gaaatcgttt atcccaaatt ttctcgtaaa aagcgattgt ttcctttgct
     3841 ttcataaaag gattatcata agttgtcgtt gctcctctac acatcataag ctggtatcct
     3901 aatccatgag caaacttcac agtcgtatct atacaatact ctgcttgtgc cccacataat
     3961 tcgatcgaat gacagtccaa ttcagttaat aaaacactca aattagtctt ataaaatgaa
     4021 ttcgcatggg tttttctcac aaaataactg tgctcaggaa tagataattc cgacaatagc
     4081 tcccatggtt ttgtttgtgg cactaattga gcatcttcgt gttgaacgaa aataactggt
     4141 ttcccaatcg tttcgtatgt tttgattctt ttattcacta atgctgtcac tgtttctaat
     4201 tcgtgtatcg gcgaagaatc aaaacatacc cctttttgta aatcgatcac tactattgcg
     4261 tcggctgtgt ttgacatact tgtgccttct ttcttttttc ttgatttaat agtatttctt
     4321 atctagctag ttccattata cgtgacaaca ttttcttatt ttacacctct aaatggcact
     4381 tttcgactat ttcttcatgt tattatttca taaatactta gctattggag gaatgtatga
     4441 tgattaaatt gtatacaggt tctggctgct cgtcttcaag acgcgcaaaa cgttggctta
     4501 tcgtccatga tataccgttt atcgaacaga acttaattcg tcaaccacta accaaaaatg
     4561 aatttatcca cttactaaaa ttgacaaaca acggcgtgac cgaaatgctc tctactcatt
     4621 ctattagtta taaaaatctt gttcacagaa tagatgaact cagtttgagt gaactatata
     4681 ttgagattcg tacatcccct tctttattga aaaaaccgat tatctacgac caatctgcaa
     4741 aacttttgac tggatttagc gaagataaaa tcaggatgtt cctttctaaa actaaaagaa
     4801 agcaagagct caacgaactc ttgctttctg gtcatcttgt cgattttaaa gaaaataatc
     4861 tttttgcaat gtcttaattt tggcataaac aatctgcttt tcttaattaa cgaaatccaa
     4921 tttttccaat atggatctat ttttctcatt cctgattttt atgatttcag ctttcagacg
     4981 cgcccaatca ttttttgtag gttgtccatt ccagtaaaat atctgtgtat tcgtttgatc
     5041 atatgccttt ttgtcgatag gataatcgct aatgacaata tcaggtaact gatcagcgga
     5101 tgcactatat tttattgggc gttcagtcat agataatatc cttttctttg cccataattc
     5161 ctgtgtttta ccatactttg attgaatata gaatatgaca ggttgttcgt gttttataaa
     5221 aatatccctg actaacatac agtattgaaa aatcaaccgt ctgttctttt cataataagt
     5281 attaagctct ggaattctgc gagctacccc actaaaaaac tgttgcatga ttggatacat
     5341 cactggaaat atatgctgaa aatctctgtc ttctgctgat tttccaaaag catccacctt
     5401 cttttttgtc ccaaaaaccc ttgaatacaa atggatcatt gccaaattga catatagata
     5461 atttttttct gaagttgtaa aacaaatctt gaagtttttt tcaaaatttt ctacaataga
     5521 cgattcaatc cagccccaac tttcttgtac tttatctgga gtatccaaca aatcttcttc
     5581 caaataggtg ttcattacac caaacataaa ataaaggtaa gcttgctctg ttggagtgat
     5641 gtgattcatc gaccagaagt ttgcttcaaa aaaaggctct agtatgtaat gcgtaaactc
     5701 ttcttttgtt atcaatggat gcacaaattt gttcatctct tctgggacat acgtaatcga
     5761 gtggccttgt ttcattcgtt gcaacgaaat tcttagtagg ctgatccatt tctttctatc
     5821 tatactacgc gtatatggcg catgtttcgc aaatttttct aatagttgac gtttattttt
     5881 tatacttgat aatatatcag aatgattttc gtcgaatgac tgccaataca tcaagtgaaa
     5941 aaagtatcgg atacggtatt cttctccttc tatcggatta ccttttttca agctgcaaga
     6001 taaatgatag tttttcaaaa tagtattaag ttctcttaat tttctttgaa tcgtacgcaa
     6061 acttatataa ttttttgact aagattttct aaggattgat aagtaccttt gtaaagtgaa
     6121 tccagtaaaa tataaccaat cgttccctct aaaagtatag aataaacttt actcagtaaa
     6181 aactgagaag aaaactcgac ttttatcatc ttgttttctg aaagaaaaga taatttatat
     6241 tctggatacc tttcgcaaaa cgattctgct tgttgaatca ccgaattcat cttagccagg
     6301 gtgtagttta gatctaaagc gatatccttc accacatata atttctcgtc tctttcataa
     6361 caatagttca aaaattgata tacttgcctt tcattgatat caataaattc ttcaaacaaa
     6421 gtacatactc ctttttatct attatttaga cttattccga cagctacatg aataacgtac
     6481 aaatagattc gttactgatt tttcagagat actaccatgc gcagtgtgca ttgtcaatta
     6541 ttcttgctct tattttccat ttgtgttcct gttatttcct gtttctcaaa tatgttagta
     6601 aacaaaaata gatctggcaa agctacaact tcgccaggca atacagcact ctcgaatgaa
     6661 atattcactc aaaaatctat cgttttattc aattttagat ctgttgcttt tattctttat
     6721 ttcactcgat ggaagagaga acgaaaatag cttgctccta caatcaaaat gagtaacaca
     6781 cccatccatg taattagctt gttcaaggtt ctactaccag tttctggtaa atcgcttttt
     6841 ctattatctg tttctttcgc tttttcttga tgatcaattc cacgagaaac aaaatcaggc
     6901 tgttttagca cactttttag gttgctccct gcatttctct tttgttcctt agttggtatg
     6961 ataggtacca aatcaagttc attatttact tttcctgtgc tgctttcgct gctcgtgctt
     7021 cctgtgctac tctcactact catgcttcct gtgctacttt cactactcgt actctccgta
     7081 ctgctttcgc tgctcgtgct tcctgtgcta ctttcactac tcgtactctc cgtactgctt
     7141 tcgctgctcg tgttttctgt gctaatttca ctgctcgtac tctctgtact gctttcaccg
     7201 ctcgtgtttt ctgtactgct ttcactactt gtgctatccg tactgcttgg cggtccaaca
     7261 actgtccaac tgcttggttt caaattctcc gtgatcgtca tctgtccatc ataaaaaaca
     7321 atcaaatcaa ctttatcttt actcttgatc tcatcaatcg atagttctgc agaaatcatt
     7381 attgtatact caataccagc ggtccattgt acgtctttta aataaacttc cccaataact
     7441 ttttctgaac ttccattacc atcccaacca tatgttagaa cagcatgtac atcactagca
     7501 ttcacgcttt tgtttggttt gactggaata gtaatgactt gttgtgtcat gtcataaaca
     7561 atccgatcag ttttcacagt aaatgaaaga ttctcatttg cccgttcatt tgcagaagta
     7621 ctgattccaa tcattgctgc aattatactc agcccaagaa taacaaataa tacggtcgct
     7681 gttttcttca tttctatcac tctccatata attaccttct atttgataaa ggaattatat
     7741 aaaatgaaaa tgatgagaac acgacagtca gttgtgaaaa tctaacaaaa gatgtcgtgt
     7801 attcgcttga atttgtttac tttttcgttt aatcaatgac agacttaaaa aagcaagacc
     7861 gatatcagcc ttgctttctt catcttttat tgataaacag cagtgtattt cttcatccgc
     7921 tttgttttat atgcatagag taacaaacca acaatcaaga ctaacaagaa taatgtagtt
     7981 gatatagatg cttcagcccc aaagtttccc ccagtaatta ttgtattttg cgattggatc
     8041 agagtggaga aaaaaccttg ttcttgcgtt ccgctgactg gtatacccaa aatagctcct
     8101 tgtgtccaat tccaagtaat atgagcacca atagccgtaa ataaacttcc tgtactgtag
     8161 ataagtgcgc tgaaaaagca acctgctaaa aagatattga aaactgccag aaatgtcatg
     8221 ttaggattca atccgtgcaa aagagcaaat aatgcagcag atccaatcat tgctattaac
     8281 ggaccaaatc taagcatctg atgctgaaaa aagccacgga ataaaagctc ttctgatgcc
     8341 cccactgcaa taaataaaat aaaactcgtt atcagcggca caatgtttgc gtttcctgaa
     8401 tctaaccgat acgcccccgt caaatataat ggactagtaa taagcagaaa cactccaatt
     8461 ccaacaacga gacctcccgc taaatacccc cagtcttttc ctcgaacaga aacaccataa
     8521 tcttctattc tttttccttc gaccttacta gcaagaaata aagctagtaa aaaaacagct
     8581 aaatgtccga ccgtaccggc aattgctcca atccattcac tatttccaat aaacatatcc
     8641 gcaagtgcat tattgacaag aattcctaaa acaaaaccaa caaagactat taaaacagat
     8701 ccaatcaacc gaataatcct gatcatcttt catccctcct ttatattaag tttaacaacc
     8761 cttttctttc ttgtctcgtg aaaactatgt aaatgttctc catcatagag agaatagatt
     8821 tttacgaact aagaatatct acagcaacct tacgaaaatg aaagactgtc tccctaattc
     8881 ttctgttaga ctgtttttaa taaggaaagg atgggaaatt ttttgaaaaa acaactacta
     8941 attatgtgcc tttcagcatg tgcaatttta ctattctcag gttgtagcaa gtccaatgaa
     9001 acgattgcaa cgtttaaagg tggaacactt acagttcagg atttttatga taacccaaaa
     9061 gttaaatcaa ataatcaaac actattaaaa aaaatgattg tcttcaaagc atttgatgat
     9121 gtatacggaa aagagatttc ttcaaaggaa gtaacaaaag aatataatga acaaatcaaa
     9181 aaactcggtc caaactacaa ggaacaatta aaagcagttg gccaaacaga agagacatat
     9241 aagaaactct ttaaacagat gctggctttc caatacggtt tgaaagccaa tgtaaaatta
     9301 acagataaag atctagacac tgcttggaaa gaattctatc cagaagttag tacacagatc
     9361 attctatttt cgactaaaga agaagcagat aaagcaaaaa aagaagcaaa tgaaggagaa
     9421 aatttctcca agcttgttca agcttatgga aaaaataaaa cattaaagga aactgatgga
     9481 aaaatgaatt ttgattcaac caatccagag attcctactg aagtaaaaaa agcagcattt
     9541 aaactaaaaa acggggaagt ttctgatatc attcctgtaa cagatccaac gacttatcag
     9601 caaagctatt atctagtaaa aatggtaaaa aaacaggata aaggttctaa taaagacaaa
     9661 tacaaatctg agctagaaaa aattgctacc gaagcaaaaa ctcttgatac ggaattcatg
     9721 gataaaacaa tccgtaaagt gatgaaaaaa gacaatgtaa ctattaaaga tccttatgta
     9781 aaaaatatat tcaaataaac aaatttgacc caattgggga tgtggcaaaa tttgagaagt
     9841 gatttttgga aattccttct tatttttgag gcaaaccact tttgccacaa cttcatcggg
     9901 gccttttttt atttgtacca aaacaaatca ctggatttgt tacaatcatg aggatataat
     9961 ttaaaaaagt tttttatttc tatcacatga atcaatgcgc cattaagtag attctttcat
    10021 gcttccctat tttttcactt gccgtatcgc ttcaaaaaca actatcataa actcaaactc
    10081 gactgaaaag ttattttaaa aaagactaaa aatatccctt ctggacttgt tcttcttatt
    10141 ctgttatact agagaaacac gaaagatatt tttatgcgac acttttcatt tgtttttatg
    10201 aaatttattc aaaactggcg taacttcaga gttacaagga tgtattagag gttagggaat
    10261 acatcgttta aatgagaaat aagtaatagt tagtaatagt cacccaaact ttaaattaat
    10321 gaatattgaa tcgtcagata gcctcctcga gttttcgaga aggctatcgt ttattttatg
    10381 gagaaattga ttcagaagta atcagcgacc ttttttagtt caccaatcgt ttctgcttgg
    10441 aaagtttgca cccaatcttc ttggaagccc ctcactgctt tatcaatcga gggcatctct
    10501 aatacttgat ctagtagctc tttacctaac gttatcgctg agctccagat cgaatggctg
    10561 tagtgacttg ttcacaattt ttgaaactag ctgctaatat ttttgttggt ctttgcgtac
    10621 gttcgatctc agtccgcaac gaatcaatca ctttcatagc atcaatgcct tgattttgca
    10681 tcctgttata ataaggtgca atataatctg ctccattatt aatagctaaa tacccttgta
    10741 attctgtgta aatagctgta ccggtaatca aataaccttc ttttttcaac tttttgatcg
    10801 cttgaaggcc tgcttgattc accggcacct tgataaacgt atcttttccc agttcttcta
    10861 tgataactcg agcatcctct atcatttctt cttccgttct tcctattgtc tgaatatgga
    10921 taggaatctg tccgattatt tttttgattt ctttcatatg cgagaagaag tttgacgctc
    10981 cttctttttt cagaattgta ggatttgttg ttacgcctgt taaattcaaa aactgttgac
    11041 aagaataaat actttttgta tttgctgtat caagcatgat ttccatatag ctttccccca
    11101 ttctgatcct cttgatcgat tttaatgatt gtcccctttc ttaaatgcgg catttcctta
    11161 tcttctacat acattgttcc tggaagttcc ggctcatgag atccatcaaa ttttatcgtg
    11221 atatgtccta attcattcaa attagtttgg acaacatctc ctactgctgt gattacatac
    11281 atttgtttat caaaacagag cttcatatca caagttattt cttttcttac ctgattcaat
    11341 tcaatcttat aacaataatc tgttaatgtt tccggtgctt catctccaaa taaaatcatt
    11401 attttttctt tcctgaatac ttctgcttct ataccaacat ctgttacaac cgtttcaaag
    11461 atcgtcatct ct
//
LOCUS       DACCXM010000087        11233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCXM010000087 DACCXM010000000
VERSION     DACCXM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.089"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            84..707
                     /gene="lexA"
                     /locus_tag="IVY61_002012"
     CDS             84..707
                     /gene="lexA"
                     /locus_tag="IVY61_002012"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP7971120.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            887..1105
                     /locus_tag="IVY61_002013"
     CDS             887..1105
                     /locus_tag="IVY61_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971121.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(1394..1906)
                     /locus_tag="IVY61_002014"
     CDS             complement(1394..1906)
                     /locus_tag="IVY61_002014"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7971122.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1920..4217)
                     /gene="recJ"
                     /locus_tag="IVY61_002015"
     CDS             complement(1920..4217)
                     /gene="recJ"
                     /locus_tag="IVY61_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP7971123.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(4359..4793)
                     /locus_tag="IVY61_002016"
     CDS             complement(4359..4793)
                     /locus_tag="IVY61_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP7971124.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4803..5591)
                     /locus_tag="IVY61_002017"
     CDS             complement(4803..5591)
                     /locus_tag="IVY61_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7971125.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(5588..6529)
                     /locus_tag="IVY61_002018"
     CDS             complement(5588..6529)
                     /locus_tag="IVY61_002018"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP7971126.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(6706..7293)
                     /locus_tag="IVY61_002019"
     CDS             complement(6706..7293)
                     /locus_tag="IVY61_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP7971127.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(7514..8827)
                     /gene="obgE"
                     /locus_tag="IVY61_002020"
     CDS             complement(7514..8827)
                     /gene="obgE"
                     /locus_tag="IVY61_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP7971128.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            9115..10125
                     /gene="gap"
                     /locus_tag="IVY61_002021"
     CDS             9115..10125
                     /gene="gap"
                     /locus_tag="IVY61_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7971129.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(10210..11145)
                     /locus_tag="IVY61_002022"
     CDS             complement(10210..11145)
                     /locus_tag="IVY61_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7971130.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3136 a         2515 c         1967 g         3615 t
ORIGIN      
        1 actaaataaa atacgaacat tcgtttgatt tagcgctttt tatatggtac tatttattta
       61 tcaagtgaaa gaaggtgtac cttgtggccc gacaaactga atcaagacaa atcgaagtct
      121 tacgatatat ccatgaacaa gtaacccaaa aaggataccc gccgactgtc cgtgaaatcg
      181 gggaagctgt tcagctttct tctacttcta ctgttcacgg tcatcttgca cggcttgaaa
      241 aaaaagggtt tatccaaaga gatccgacga aacctcgtgc aatcgagttg acaaaacaag
      301 gtttagaaaa gattgggatt cgtccaacaa ctattccagt acttggcgtc gtaacagctg
      361 gagaacccat tttagcagta gaggatgctt cagacttctt tccaatccct cctaatcttt
      421 cttccgaaga aggcagtctc tttatgctga caattcgtgg agatagtatg atcaatgctg
      481 ggatattgga tggtgacaac gtgatcgttc ggaagcaaga aactgctcaa aatggcgata
      541 ttgtcattgc aatgactgcc gaggacgaag cgacttgcaa acgattctat aaagaaaaaa
      601 actttttccg tctgcagcct gaaaacgact tgctcgaacc aatcatctta gatcaagtat
      661 ctatattagg acgagtagta ggactttatc gtagtcatat ctattaattg ctactaatta
      721 aaagaaaact ttttaaaaag agtttgagac gttggtctca aactcttttt tcgcccgggt
      781 tcttcttctt tcaaattttt atcacttccc tctttacaaa ccgaacaaat attcgtatac
      841 taaatatacg aacaattgtt cgcataaatt aaagaggtga gaattcatga aatcaatgag
      901 acgtcttggc ttagtattaa ctgttttgtt tttaggtatt attattggta atttcggcat
      961 acgtccagct ttacttgtgt ttgttccgtt atttactttg tggttcatgt tgtgggatga
     1021 aaagaagtac cgtcaatctg agcggcgcag aattcatgaa gatcatttct atcatgagcc
     1081 ctactacaga tccaggcaat cataataagg attgactgct aattattcaa aagacaagaa
     1141 gttaaacttt tacgggggtg tgacaaaaat cggtcacatc ctttttttcg aataaacggt
     1201 gttctaaagc cagtttttcg gtattaagtc aaattgcgga aaaagtgatt ttttaaactt
     1261 ctactggtct ttgcttgttt ccatttataa aaaaatagcg accctcaaaa gtcagataga
     1321 gaatctgact tttgagggtc gctttaaaat cgcttcttta agaataacat caagaatagc
     1381 ttcttttttc tgcttaatat tccattaatg taatgatgtc atatcctttg attttgtcgc
     1441 gtccatggag atccatcaat tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt
     1501 gttcgatcag ttcgatcgtc gctttgattg ttcctccagt agctagcaaa tcgtcacaaa
     1561 tcagcacacg ttgacctggt ttaatggcat ccttatgcaa tgtcaatgta tctgttccat
     1621 attctaatcc ataagtcact tcgatggttt ctcgaggtaa cttccctttt ttgcggactg
     1681 gggcaaaacc gactcctaac tcataagcaa caggacatcc tacgatgaat ccacgggctt
     1741 ctggcccaac taccatatca attcgttttt cttttgcata atttacgatt tgtttagtag
     1801 cttcccgata agcgtcacca tctgccatca gtggggaaat atctcgaaac acaattcctt
     1861 ttgacggata gtcgggaata ctggcaatat aatcttttaa atccactctt tgtttcctcc
     1921 taggttgtta accatttctt tatggtagga atatcgctca aaagcaaaaa ttcctcaact
     1981 ttgattttat tgagccgtcg ctggtataat ctgctctcag tcaaaggatg actttctggg
     2041 ctagcgactt tttgtaaaac accgttctct attgtaacaa atttcaattc aaaaaacacc
     2101 tgtatcataa aaattaataa tttttcaggg atacgcaaat attgagctat tgcttttatt
     2161 ttatgacgta tatcaattcg ttcttgagaa tggatcaatt taaacaattt tgcgtactgt
     2221 tctcttgtgc ctaccccatt caaataggcc tcatcaggtg aaatccccat cagataaaca
     2281 cgattgaatg aataagaccg aaaaacttct ttcagatccg ctaaatcatc tggacaatcc
     2341 ataacaacta gagagtttgg ttttcttttt tcaattattg cctttacaga atctagtcct
     2401 tcgaaaacaa tcgcagcttc ctggataatc tcaggcacga atttcaacgt cgaagaatca
     2461 aatgctaaat aaaggctacc ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc
     2521 caatcaaata cttgcagacc acccacagaa aaatcagata ccatcagctg cggtttctta
     2581 cgcccgttcc attcattgat cgaaagcttt cctaccacat ctacctgatt attcagcagt
     2641 tcttcttctt gagtaccaaa accaaaagcg accgcatcaa gctgactcga tgcatcggaa
     2701 aggacaaatt tcagatgctg ttggtttgct ccgattcttt tgatattttc tgcttggact
     2761 tgtcgaaata aaaactgagg caaaggatta tccgtaccaa atggcgccaa tagtttcagc
     2821 tcatcaattc gctctactgt tacttcattt ggcagcaata cttcatcaat tctcaactca
     2881 ggccctctca tcaagtcaat ttgatgatcg actatatatt ggttcatctt ctcctgtagt
     2941 atagtgacat tttcacttgg catcgttaaa ccgactgctg catgatgtcc tccgaagtaa
     3001 gtaaataaat ctcgcatctg gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt
     3061 ccagatcctt tagctgatcc atcttctttt aaagtaagga caagcgttgg tttacccgtt
     3121 tcattcatta ttttcccagc aacaatgccc aaaacgcctt catgccatcc tcggtttacc
     3181 aatagatgga tatgattctc cttattgacc atcgctaatg cttcttgtgt tatctgctct
     3241 acgattgctt ttctttcttc attgatctca ttcaattttt ttgcttgaac ggatgcttcc
     3301 tcttcatcaa acgtcgcaag taacgagacg gcaggacgtg gatcgcccat ccgaccgatt
     3361 gcatttagtc tgggagcaat cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt
     3421 acgccgcttt cttcaaatag cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg
     3481 attcctaatg aaacaaggat tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt
     3541 cctatcgcta ctaaatccaa aaattctgca ggcggttctt ccaataatgc agaagctact
     3601 ttgaatgcta ctccgactcc tgccaaatct ggaaatggat agtgccccgc tggatgacga
     3661 gggtgaacaa tcgcataagc gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg
     3721 atcacgtcta caccagcttg ctgagcgtat gctaccgcat catttcctgc taccccgtta
     3781 tcaacagtga tgatcaactg gattccttct tcgatctttt cttggtatac cgattggttt
     3841 ggtccatacc catgctcaaa tcgatttggc aggaatactt ctacttctgc accaagtaat
     3901 tccagtgttt ctttcattac cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa
     3961 acgaggattt tttctccact gattacagct tcttggattc ggctgattgc tttatccata
     4021 tcgtgtatca gatacggatc atgtaattct tgttcctttg gatgtaaaaa tcggttgata
     4081 tccttttcct catggtaacc tctttgccac agtaacttac cgacaaatgg agataatttc
     4141 agttcacgga tcatttggag aaaggattca gaaggctctg tctcgcttaa cttccaatca
     4201 aaatttgctt gtctcacttt ctcactccta acttagttat tatagcaaag gaaatcagca
     4261 aaaaaaagca gaaagaaaga ataaaaacag atcagatccg ctagacagat gttcagcagg
     4321 acctgatctt ttgtacgacc atttatgaaa tatccctatt agtcaaaata attgaagctt
     4381 tgtttgggcg attgagtttt ttgttcttcc ggcgctttca attgactgat ttgtgcggtt
     4441 ttcgcttgta actccgcctg ataagcagct tccaactccg cacgttcgtt tctaaactcg
     4501 cgttgctgtt cttcttttgc ttcttcaatt ttcttttgaa gttctgtttg atattctgac
     4561 atttcctgag tcagctgttt gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc
     4621 aaaaatacaa tcagtgcacc aatcagcgca gaaccgatga tcactaagat caatggcgca
     4681 gaaaaggaag tgaagccaaa acttacagga acatttttat tgttcaatac agcaaaaaga
     4741 acaataatca aaactaaaac aaatccaaga atcacattgc tttgcttttt caattccttc
     4801 acctactttt tgttaaaaat tccgctggct aaataatcac caatcgttgg aaacagcgta
     4861 taaaattttg atgcaacttc taaaataaac ggttgattga tttcccgttt tggatgtctc
     4921 atattgcgga cgatcatttt tgccaacttg gcaggttcta aaacgatttg tccgactttt
     4981 tccaaatagc ttccgcttgg atcagcctta tcgaagaaat ttgtctcgat tggacctgga
     5041 ttcactgtcg taacagctac gccaagtggt ttcaattcta atcgcaaagc gttggagaag
     5101 ccaaggactg caaatttcgt ggcagaataa accgttgatt ttgcagttgc catttttcca
     5161 gccatagagg cgatattaat gatatgacct tgtcttcttt ctgccatatc gatcgcaaat
     5221 ttttgtgtca aaaccatcat tcctaagatg ttgacttcaa acatatcgta tgcttttcca
     5281 aggtcgaatg taaggaagtc ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg
     5341 acgcgcccga cttcttccat caccttttca tataattcgt ccacgctttc aggattgctg
     5401 acgtctacct tgaaagcata agcctctttg ccgctcaagc gtctgcactc gtctctgaca
     5461 ccttctacaa atgccattct tcgtgcgcaa cttataacga ttgctccttg ctttgctgct
     5521 tcataacaga tttgtgcgcc cagaccgcct gaactacctg ttactaaaac aactttatct
     5581 tctaaattca tgcctcttcc tcctcgcgaa atggaatctc aatgctatca aaatctttca
     5641 taatcgtcgt gttttcaaac acttcttgtg cttctttttc aagctctaaa gctgcttttc
     5701 ctaaatatct cgcactgata tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa
     5761 cttcagcagc ttgatgagtc gtggaatgga aatagttgtg cgccattctt gcttcatctt
     5821 tattaaatgt gctttcatgt accagaatat cgctattttc cgcaagcaca acggaattat
     5881 gtgtttttct cgtatcgcct aaaatagtca cgattcgtcc ttttttatct ggacctacga
     5941 aatcttttcc attgatcgtc cgcccatctt ctagttggat cgtttcacct tgcttcagtt
     6001 tgccgtacag tggtccagct ggaataccta attcttttag acgatccacc tgcaattctc
     6061 ctttatgatc atgttcaacc acacgataac caaaactgtc gatcccatga tttagttttt
     6121 ttgtataaac agaaaattgc tgatcagtaa aaatcggggc tgtttcattt agctcaatga
     6181 atttcagcgg ataacttaaa cgcgtttgcg aaatacctaa agagactttt atgtattcct
     6241 ctatcccttt tggcccgtaa atctctaatg gcgtatcccc accttgaaat gaccggctgc
     6301 tgatgagccc gggaagtcca aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac
     6361 cgatttttct cggacgaata gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt
     6421 cgaacaacca aacttcattg cgctcgtcta aaagtttcaa tgctatactt gtcacatttc
     6481 tatgttttgc aggaacacct gcacctgttc ctaaaaattg taattccata atctacctga
     6541 ctttctttaa gttcctcttt aattttaggg taaaaagaag ggataatcaa ccgtaagtcg
     6601 agaatgggtt caaattaaaa ataacgttct aaagcgatag cgttttcttt gaaaattact
     6661 ataaaaacag cagcgaaccc gtttgagatc ctctgctgtt ttttattaat ttgaagatcc
     6721 agaactttca ttgtcgttgg aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc
     6781 ttctttcgca tgattttcag cttctttcgc atcatgcaca tctttttcga catcttcata
     6841 atccaagcga gtaatttctc cgcctaattc catcattacc aattgattca gtgggcgatt
     6901 gtcctcttca tcagcaatca agatcagtcc tgtagctcct tcaccgattt tatttcctac
     6961 aaagtcgaac acggtctggg catcttttat ttctttggca tcttgtgtcg caccgatcat
     7021 tcctccggca aaccatccta ataaaatccc tagcggacca cctaatacac caaccaacat
     7081 tccgattaat ccacccgtag aagatttgtt attaccggta aagtcgataa aatcttctat
     7141 tttgaattga tgtgtcccat taggatcatg gctgacaact gccatctgtt ctcctttaat
     7201 cgaacgaaca gcatgtaact ttttgatttc tgaaaatgcc tgatatgcct ggctttctac
     7261 ttgaaaattt aaaatgatta ctcgtttgct cattaaaaac gcccctttca taccttcttg
     7321 ttatttattt tacctttaat tgaaaaataa gaaaagaaaa gcactctttt tttccaataa
     7381 aatagggctg tgacaaaagt cgtatcacag ctcctttttc cgaataaacg gcgttcttga
     7441 agctgatcac ttctgtcaca acctccctta aatctgaatc tatatttttt ggtatttgtt
     7501 tttatttgct tgcctattcg acaaattcaa attcaaactc accgatacgt acaaggtcgc
     7561 catctttcgc accgcgtgcc cgaagagctt catccacacc cataccacgt aattgacgag
     7621 caaaacgcat cactgtttca tcatgatcga aattcgtcat ttggaataat ttttcgatct
     7681 tctcaccaga taagatccac gttgcatctg aatcacgatc gatttggaac tcaggacctt
     7741 ctggattgaa tccatagtgt acagtttctt cttccagttc ttcttcgtac aatgggaatt
     7801 ctggcgttac ttctaacaaa tctgctgtag cattcaataa cgcgtccaat ccttgtcgag
     7861 tcactccaga aattggaaat actgggatgt catcagcaaa ttcatcttct ttttccttgt
     7921 tcagctgttc tttgaatttc actagatttt ccggtgcatc cggcatatcc attttgtttg
     7981 ccacgatgat ttgcggacgc tctagcaagc gtaagttata agtagataat tctttgttga
     8041 tggcaagata gtcttcataa ggatctcgtc cttccatccc actcatatcg atcacatgta
     8101 aaattactcg tgttctttcg atatgacgaa ggaattgtgt acccagccca acaccttgcg
     8161 aagctccttc aatcaagcct ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag
     8221 taaccattcc tagattaggt acgagcgtcg tgaaatgata agcgccgatt tttggacgag
     8281 cagatgaaat aacggatagt aaagtcgatt tcccgacaga tggaaaacct acaagtccaa
     8341 cgtctgccaa tactttcaat tctagttcga tttttcgctc ttgtcctggt tcaccgtttt
     8401 cagcaatttc cggtgcagga ttccgaggag aggcaaaacg gatattccct cgtccaccgc
     8461 gtccaccttt tgccacaact agtgtttgac cattttcaat taaatctcct aaaagagcac
     8521 cagtctccgc atctcggaca gtggttcctt gtggtacttt aacataggta tcttctgaac
     8581 cacgaccgtg catcccttta ctcatcccat tttcacctgg ctgcgctttg aaatggcgat
     8641 tgaaccgaaa atccatcaat gtacgtaacc cttcatcgac gatcaagatg acatcgccgc
     8701 cacgaccacc gtctccgcca gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg
     8761 caaccattcc gtctccgccc ttgccagctt tcacatcgat cgttacttga tctaaaaaca
     8821 tggacataat taagtcctcc tattattcat ttatttattt gactgcctga atttccacaa
     8881 taaacaaaag tcttacttat tttatcggtg aaatctgatt ttgtctatca tttcagcaaa
     8941 aagattgttt accttcttct ttttttatca tttggtaagg tttccaacct tttttctgct
     9001 accaagtata attttttgac aaaatattct ttgcgtagca gaataacagt gtattaattg
     9061 tattaattga ggatgccgtt tcacagcacc cctaataaag gaggaacttt tgatatgaca
     9121 gtaaaagtag gtattaacgg atttgggcgt attggccgtc tggctttccg ccgcatcaaa
     9181 gaagtctcag atgatattga agtagtagca atcaacgact taacaagtcc aactatgtta
     9241 gcacaattat tgcaatttga ctctactcat ggtacttatc ctggcacagt tactgcaaca
     9301 gaagactcaa tcgtagtgga tggcgaagca actcgtgttt atgcagaacc cgacgcaagc
     9361 aaaatccctt gggcaaaaga aaacggtgta gatatcgtac tagaatgtac tggtttttat
     9421 acatctgaag aaaaagcaaa agcgcattta gatgctggtg tcaaacgagt agttatttct
     9481 gccccagctg gtgctatgaa aaccatcgtc tataacgtca atgatgatac attagatgaa
     9541 aacgacaaaa tcatctcagc aggttcatgt acaacaaact gtttagcacc tatggcttat
     9601 ttcttaaata atgaatttgg gatcgaagtt ggtacaatga caacagttca tgcctacact
     9661 tccacacaaa tgctcttaga tggacctgta aaaggcggta atttgcgtgc tgctcgttca
     9721 gctgctgaca atactatccc acattctact ggtgctgcga aagcaatcgg cttagttatc
     9781 cctgagctac aaggaaaatt acaaggacac gcacaacgtg ttccagtcgt tgatggttca
     9841 ttaactgaat tggtttcagt attgaaaaca aaagtaactg ctgatcaagt aaacgaagca
     9901 atcaaaaaac acacagttga caatccttca ttcgggtatg atgaccgtca aatcgtttct
     9961 agcgatgtca ttggaacaac tcaaggttcg atctttgacc caacgcaaac agaagtaaca
    10021 actgctggtg atttccaact tgttaaaact gttgcatggt atgacaatga gtatggattt
    10081 acttgccaaa tgattcgctt actagaaaaa ttcgctaact tgtaatttga tagatcaatc
    10141 gataaaagaa gcctttgact ggatccagtc aaaggcttct ttcttttcat cccctacttt
    10201 taaactcgtt caatgaacat attggcttaa aaattgttgg actacttgct gatatttttc
    10261 aggatctgtc ttgtatgctt ccgcatgctc ggctcctttt acaacatact tttcttttgg
    10321 tcccttcgtt gcacgataga ccttatctaa catttcaaat ggtacaaatg tatcttcgtc
    10381 tccatgtata aagagcattg ggcgctggtt tttcttcaat tgttccactg catttgcttc
    10441 accgaaaaag tagccagcac ggacttttgt cactagactc gttacttgca taagcggaaa
    10501 tgctggaaga ctaaacatat cgtctagctg ataagcaagc tcatcatgaa cggaagaata
    10561 accgcaatct tcaatgattg ctttcacatt atctggtagt ttttctccgc tagtcatcat
    10621 aactgttgct gctcccatgc tgacaccgta tagaacgatt tcttgcgatt ttccattgtt
    10681 ttctagcact ttgtttatcc actgtacata gtctttacgc tctggccatc caaatccaat
    10741 gtaatcccct tcgctttttc catgtcctct tgcatcagga acaaggacat tgtaacccat
    10801 atcatgaaac atctttgcgt aattggtcat cgtttcagcg tttcccatat agccatgagc
    10861 cacaagagct gttttttcac ttttcgtttc agcaggaaga taaatcgctg ataatttcag
    10921 accgtctttt gaagtgatgg tccaatcgct tcggttttct tttgaactaa accatttatg
    10981 tgcatccgtt tctttggatg tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt
    11041 ctcagaaggc acaacagcat aattataaaa ataattccct gcaaacacta acgcaacaat
    11101 cagagctaaa gcgacgccta aaaggatttt catccaaagt ttcataaatc atccatctca
    11161 tttctctttt ttcatataac cttgaattag tataatcaaa gaatctctac tatgcaatgt
    11221 tcatttttat aac
//
LOCUS       DACCXM010000088        11216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCXM010000088 DACCXM010000000
VERSION     DACCXM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.090"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            205..1140
                     /locus_tag="IVY61_002023"
     CDS             205..1140
                     /locus_tag="IVY61_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP7971131.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1230..2333
                     /locus_tag="IVY61_002024"
     CDS             1230..2333
                     /locus_tag="IVY61_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7971132.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2346..3299
                     /gene="menA"
                     /locus_tag="IVY61_002025"
     CDS             2346..3299
                     /gene="menA"
                     /locus_tag="IVY61_002025"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP7971133.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(4234..5580)
                     /gene="gor"
                     /locus_tag="IVY61_002026"
     CDS             complement(4234..5580)
                     /gene="gor"
                     /locus_tag="IVY61_002026"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP7971134.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5714..6955)
                     /locus_tag="IVY61_002027"
     CDS             complement(5714..6955)
                     /locus_tag="IVY61_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7971135.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(6973..7896)
                     /locus_tag="IVY61_002028"
     CDS             complement(6973..7896)
                     /locus_tag="IVY61_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7971136.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(8117..10609)
                     /locus_tag="IVY61_002029"
     CDS             complement(8117..10609)
                     /locus_tag="IVY61_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7971137.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10616..11083)
                     /locus_tag="IVY61_002030"
     CDS             complement(10616..11083)
                     /locus_tag="IVY61_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP7971138.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         3304 a         2283 c         1967 g         3662 t
ORIGIN      
        1 tctgttcttt tgtctaactc cttttggatt tttttcaaat tttaaaataa agcgtttttt
       61 cacgaaattt gctgaaagaa aacgtttgaa atattacatg ggttatttat caaaaatagt
      121 tgacttatag gctaaaaaga ttagaatgta taatgaatca caaagaaaaa ttgtggacta
      181 gaaatggaat ggtggggtat taaaatgaag aaaaaacgtt ttgtaactgg atttgccgta
      241 ttagcatttt cagctctagt gttaggcgct tgcggcgcag atgacaacgg aagctcaaac
      301 tcttcttctg aatcatctac agcacaatct tcgactgcaa aaagcacaac agaatcatct
      361 gcaaaagttg tagctggcgg agatttgcaa gacggcacat ataaattaga agaaaaaaat
      421 tattcaaatg gttaccgtgc agtctttgaa atggttgtaa aagacggcaa aatcactgaa
      481 tcaaaatatg ataacgtcaa tgaaaacggc gaatcaaaaa caaaagatgc agaatacaac
      541 aaaaacatgg aagcaaaatc tggcacaaac ccagaaaaat acattcctga gttgaatgac
      601 caattcttaa aagcacaaag cgcaagcggt gtggaagtcg taactggagc tactcactca
      661 tctgaaagct tccaaaacta cgcacaacaa ttgatccaag ctgctcaagc tggtaacaca
      721 gatacaatcg aaatcgacaa tggtgctgat ttgaaagacg gcacttacaa gcttgaagaa
      781 aagaactact ctaacggcta ccgtgtgcaa ttcgaaatga cagttgctgg cggcaaagta
      841 actgaatcaa actttgatta tatcgacaaa gacggtaaat caaaacaaga tgatacagaa
      901 tataatgaaa atatgaaagc aaaaagcggt acagaaccaa aaacttacat ccctactctg
      961 aacgacgaat tcgtcaaagc aatgggcgaa gaagatggct cacctgctaa tgtagaagtt
     1021 gttacaggtg ctacacacag ctcacattca ttcatcatgt acgcacaaca attagtgaat
     1081 gcagctgaaa aaggcgacac tcaaacaatc gaagtagata atatcgtaac agaaaaataa
     1141 tcacataatc atactaataa aagttggagg gtgggacgat agccagatta ggcagtcgtc
     1201 tcacctttca aaatgaagag aggaaaaaga tgcggaaaaa actactattt tccttaatca
     1261 cactgggcat ctcttttatg gtgctaactg gctgcagcaa ctcagcaaat aaaacaacca
     1321 ctgaaagttc agcagctacc aagatattga aagatccata ttctgatgaa cagttcttgt
     1381 tagggactta cgttcgtatc cgtgtgtatg atgaaggcaa agaatcagca ttgaaacctg
     1441 ccttcgatcg agtaaaagaa cttggtgata aaatcacgat caatcaaaaa ggttctgaga
     1501 tcgatgaagt gaatgagcaa gctgggatca aaccagtaaa agtttctgat gacgtctaca
     1561 cgttagtcaa gcgggcctac gaatatagcc aagattctca aggcggattc gatatggcga
     1621 ttggtgcaat cacacaatta tggcgaatcg gttttgatga cgccagaaaa ccttcccaag
     1681 aagaaatcga tcaggcattg aaattagtgg attatcataa gatcgaacta aatgacaaag
     1741 aaaaaactgt ttatctaaaa gaaaaaggaa tgatcatcga tttaggtgca atcgccaaag
     1801 gttacattac ggacgaagta gtgaaagtct tgaaaaaaca aggagtgacc actgccatcg
     1861 ttgacttggg cggtaatgtc tatgtcttag gccacagccc aagaggcgaa aaccaagact
     1921 ggacagtcgg catccaagat ccgaacatgg ctcgcggatc tgttttaggt tctattaaag
     1981 aacggaacaa aacattagtt acctctggaa tttacgagcg ttatttagaa gtagatggca
     2041 aaaaatacca tcatttgttt gatccaaaaa cgggttatcc gttcgacaat gatatcgcaa
     2101 gtgtcacgat cataacagat aaatcaattg atggtgacgg gttgtctaca gcagtctttt
     2161 caatgggtgt gaaaaaagga ctggaatacg tcgaaagcga attgaataac gggacggaag
     2221 cgatcttcgt gacaaaagac gataaagtat atgtgaccga tccaatcaaa gacaccttca
     2281 aattatcgga ggactcccat tacacgatgg gtgaccgttc tgaactaaaa taaggagtac
     2341 gaaccatgac actaaaaatc tttttagaag tagttgaaat acgaacaaag gtggctagtg
     2401 tatttccttt tgcggtaggg gtattattct ctatcgcttt ttttcatgag atacattggc
     2461 taaacacttt tttatttttt attggaatgc tagtttttga tttagcgact accgctatca
     2521 ataattacat ggattatgaa aaggctcatt ctgaagtata taagtatgaa gaaaatgtca
     2581 ttggccgcag aaatctatct cctgtattga ttcgaaacat gattttagct atgattgcat
     2641 ttgtttttgt tatcggcgtt attctaacga tcaatacggg ttggctttta cttttgatgg
     2701 gatttatctg ttgttttatt ggagtattct atacattcgg accgattcct ttatctcgaa
     2761 tgcctttagg ggaaattttc agtggggtga ctatgggcct tggaattttt gccatggtcg
     2821 tttatgtcaa tacattcggt tcagatgtct atgatctgac agtcaacttt tcatctggac
     2881 aatttgcttt ggtaggaaat ctttgggcta tagcagcaat cgtattagct tccttaccct
     2941 tggtgtttac gattgccaat atcatgttgg ctaataattt gcgggatctt gaccgtgata
     3001 ttgaaaatca tcgctataca ctagtttttt atatcggacg tcctgcggga acagtcttat
     3061 tccagctatt gatgtatgct tgctatttcg tgattttaat ggggttgttg agcggtatct
     3121 atgaatggcc gatcttggtc accttcttga ccttgccaat tgtgaagaag catttggatc
     3181 aattcaagca aacgttaccc cagcctatca gtttcagcta ttccatcaaa aatatggtct
     3241 tattcaatgc cagttatgta ttggggctat tactaacaat cattatgcaa gccatttaac
     3301 agcatgaaga ggttgtgaaa aaactgccct gcatcaagta ataagaacag ccaaataaaa
     3361 atagctcctt atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
     3421 ccgtttatca ggaggttgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
     3481 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
     3541 caccgtttat cagaggttgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt
     3601 gaaaatggct ttccacattt ttgcataaat ttggcttaat accgagaaac tggcttttga
     3661 acaccgttta tcagaggttg taaaatcagt gttttgtcct gagtattaga gaaacaaatg
     3721 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgaaggac agctgattga
     3781 acactatttg ttcggaaaaa gtgactaact atgacacaat ttttttcaca gtcccttttt
     3841 tcacttgatt tgattcgtac cagcgaactt gacgagaact ttcttggttt ggtaactata
     3901 tggtattctt ttaacatcct cgtttgcttc ttctaattta tttatacaaa gtcacacttt
     3961 tttaaacatc tgttcttcga tacatttcta tttcttttaa acatcgatac caatattttg
     4021 aacttaagag tgcataccaa atggattcat ttactcttcg tcagttacga agataatcaa
     4081 aatctccaac ttccctactt atttcgtttt gagaattatc gaagcaatct tacataaata
     4141 actaaaaaac aaaaaaccag aaaatcagta tactgatttt ctggttttac atggtttgtt
     4201 caataaaaag ctgttggaac tgttaatatt aaattatcgc atcgttacga attcttccgc
     4261 tcctgttgga tggatggcta ctgtattgtc aaagtcttct ttagttgcac ccatcttgat
     4321 agccacggca aaaccttgca gcatctcatc gacaccaaca ccaatagcat gtaagccgac
     4381 gattctttct tcttttccga cacagatcaa tttcatttcg catttttggc gataatcatt
     4441 taatgcaaag tacattgggg taaagcgtga acgataaact ttcaattcat tttcgccata
     4501 tttttccaat gcttcttctt ctgtcaaacc aattgttgca attggtggat gcgtaaagac
     4561 gactgtcgga acaagtttat agtcaagata agaatctttc tttccattaa ataaacgttc
     4621 tgataaacgt ctaccagctg cgattgcaac aggagtcaga tctaatttac cgatcacatc
     4681 accaacagca aaaatatgtt catccgttgt attttggtat ttatctactt tgataaaccc
     4741 ttttttgtcc aattccacat ttgtattttc cagacctaat ttttcggtat tcggtacacg
     4801 tccaccggca aaaagtacac aatcaccagt taaggtttca ccattttcaa atagcaccgt
     4861 atattcgtca ttgtttttag taatttcttt agcaatatga tgtgcgtaag tttttattcc
     4921 agcttcttgg tacatttgga tcaaattgtc cgaaagcatt ttatcaaacg ttctaagtgg
     4981 tcgttctttt cgatatgccc acatgacttc actacctaaa ccacgaaata cacctgaaag
     5041 ttcagccgca atgtatccag cacctaaaac gattgctctc ttaggtaact ctgttaattc
     5101 aaaaaatcca tttgaatcaa ttgcgtattc tccaccagga atggtcattt tacttgcacg
     5161 gcctccagta gcgatcaaga ttttaggagc acgataggtt tcaccgttta cttcgatcgt
     5221 ttgactatcg ataaaagtag cgtaaccttt tatagcctcg acatgattgc tatctaatcc
     5281 tctttgataa gcaccgtgta gaaaatcaat atatttttct cgtctttcta ctagaccggc
     5341 gaaatcaaca ttttttaaat ctgcttggat accgtaactt gaggcatctc gttcaattgt
     5401 ttccaaaatc gtacttgctt gccacataac ttttttaggt acacaaccga cgttgacaca
     5461 tgttccgccc aattcattcg cttcaatcag caaaactttt gcgccatgca tccctgctct
     5521 gtttgctgaa gcaataccgc cagagccgcc accaattacg atgtaatcat atatttgcat
     5581 tttgttcctc cttattgaag agatcaatca aaacctttat ttaacgatcc tttttctaca
     5641 gaaaacaagt gtaacacttt tcttgaattt tgtatgcgaa gatgcttaaa acagggatat
     5701 atcagctttt tccttattta cgttttcgga tcatatctcc aggtttcacc ggctgtgcga
     5761 ctggcatagt taaaatcatc attggatttg gtgctcggtc aatggattcg ccttcttcat
     5821 tatgcataac ttcaacggtt tgactgaaat gactgaatcc tggaccataa aattcgattt
     5881 catctcctac agaaaacaag tttctctgac gtatcgtagc aatctgtgtt gcctcatcat
     5941 aagataatac ttcaccgaca aatttatata ctgggatttt acgacgttca ccgaataatt
     6001 gttcgttttc agttggtgta ttgtagtaaa aacctgtagc taattctcgt tgtgctactt
     6061 tccataactc gtcgatccat tcttgcttgc agacatagtt ttcaggatct tccatataac
     6121 tgtctacagc cgctttatag acatttgaca ccgtagatac ataatggatc gatttcattc
     6181 ttccttcgat cttgaagctg tctacgccgt tttcaatcaa ttctggaata tgttcgatca
     6241 ttgacatatc cactgcactc atcgagaatt cttcttccac ttcgcctgta tcagttaaac
     6301 tgtttcgttc ttgaccaaaa ggcatatcga acaatccata cttccagcga caagattgag
     6361 aacatccgcc acggtttgca tcccgcatcg acatatggtt ggacaatgtg catcgaccag
     6421 aataagagat acacatggca ccatggataa atgcttcgat ttcggtatcc gtatttttac
     6481 gaatctctgc cacttcctcc attgaaactt cacgagctaa aacgacacgt tccaaccctt
     6541 cttctttcca gaattccaat gtctcaaaat tagttgctga agcttgagtg gataaatgga
     6601 tcggcagacc tggggcttct gtcgcacaaa tttcgatcaa agctggatca gaaacaatca
     6661 ctgcagaaat accgacatca cgcaaactgc ggaaaaattc ccctgctcca tcttcatttc
     6721 cttcatgggt gaccatatta gcagcaacgt ataccttcgc atcatgttct tttgcaaaag
     6781 caactccttc agccatttct tcataagtaa aattacctgc acggcttctt aaaccatatg
     6841 cattcccgcc aatatatact gcatctgctc cataatggat cgcggttttt aacttttcca
     6901 gtgttcctgc tggcgctagg atttctggtc gtttcaattt tctttttact gtcatttctt
     6961 tcatcatact ccttatttga tagcagaagg atcaatataa aagaatcctg tatctaattc
     7021 acggttttca ggatgcaatt tactgatttc gctagtcatt ttttccgctt tttcttgatc
     7081 ccagctacct tctaaaattt gtttttttgc ttcggcgaat agtcgagtga tgtttacgaa
     7141 tttttcgcct ggagtaaata atccgtccaa tttccatgta cagtaatgat gctgataaag
     7201 ttcattcaat tcattcgaga gattcagatc attgctggca aaaatatgtg tgccatgact
     7261 atcttcataa atagaataat gtgtctcttc ttttttaggt tccgagataa aaagacctct
     7321 ttcacgatct ttttgttcgt cttgtttcgt atagttatag taattctgta gcagtggacg
     7381 tttggattga tggatgcatg tagcaccata cacaagggtt tctacaggaa tatcgagttt
     7441 ttcttccatc gccttcatct cttcaaaagg aacttcacga gcaagtactg ctccaactgc
     7501 gccttttttc gcccagaaat tgatttgacg tgcgctcgtt actaatgttt cagcatcata
     7561 aatatacggt atacgcaatt caggattttt tttcatcgta taaataatac ctgtatcacc
     7621 aactgtgatc acatctacgt ttatttcttt taaaaaagcc aaatattcag gtacattttt
     7681 cattttttca ggatgaatga tcccatttac agcaatattc gcttttttac catacttgtg
     7741 tgctaattct accaattgtc gctgttcttc tcgtgtaaat gaagcaggca gacgcaatcc
     7801 aaattgctct tcaccgaaat atagtgtatc tgcacctgca gcaagcaact gttctgcttg
     7861 ttcaatagtt tctactgtgg ttatgagttc gatcataatt ttctcctcct acaacttcat
     7921 aatagtagca tagctctaaa aactttccaa tacttcttct atttaaagtc ggatttttga
     7981 aaattatttc acaaaaaatc aaaataaagt tcgatatatt ctgtccaaat ataaaaagag
     8041 attgtgacaa aaatcttgtc acaacctctt aatcaagtct tactattata aaaattcagc
     8101 tacacaggcc aagtaactat agggaaccta ctgtttcctt tttaggtccg cgaacattca
     8161 atgtgatctt accttttgaa gcacctactg ttacgtggtc acctaaacga atttctccgc
     8221 caagcaaagc ctcgctcaat cgatcttcaa tttctttttg aagtgcgcga cggattggac
     8281 gtgcaccata ttcaggatca aatccagctt tcccgatcac atcgattgca gatggtgtga
     8341 ttttcacttg aatatcttgt tctttcaatc gtttgataat cgctttgctc atgatttgga
     8401 cgattgtatg gatttcatct ttactcaatg aatggaagac gacagtctca tcgattcgat
     8461 tcaagaattc aggtcggaaa gctttcttta actcttctaa gatacgtttt tgcatggctt
     8521 tgtgatcttt agtaacatct tggacattga atccgacatt tttttcttct cgaatttgtg
     8581 tcgctccgat atttgaagtc ataatcatga ttgtatttct aaagtccact tttctacctt
     8641 tagaatctgt taaatgacca tcatccaata cttgcagaag taaattgaaa acgtctggat
     8701 gtgctttctc cacttcatct aagagaatca ctgaatatgg tttttgacga attttttctg
     8761 tcaactgtcc accttcatca taccctacgt atcctggagg agacccgatt aatcggcttg
     8821 tactgtattt ttccataaat tctgacatat caacacggat cagggcatct tcacttccaa
     8881 acatgacttc tgaaagtgct ttggccaatt ctgtttttcc gacaccagtt ggtccaagga
     8941 acatgaatga accaattgga cgatcaggat ctttcaatcc gcttcgtgca cggcggatcg
     9001 cacgtgaaac tgctttgaca gcctcatctt gaccaacgac acgttcatgc agaagtcctt
     9061 ccagttctaa caagcgttcg ctttcttttt tctccaactg ctgtagaggg acaccagtcc
     9121 actccgatac aactgtagca acatcttctt ctgttacacg atcagaataa ccttgctcct
     9181 gttttgcttc aattgcagaa actttttgca gctttttacg tagtgctttt tcttgtctgc
     9241 gtaattgagc agcattttca aagtcttgtt tttggatcgc tgcttccttt tcttgtacta
     9301 gttcatcgat ttctagtttg atcacagttg attttgttaa gtgatcggtt tgatccaaac
     9361 ggactttcgc tgctgattca tcgatcaaat caattgcttt atcgggtagt tgacgagaag
     9421 tgatataacg aacagaaaga ttgactgctg ctcgtacagc ttcatctgtg atctccactc
     9481 catggtgctc ttcatatctt gtacgtaatc cttgaagaat cacttctgct tcttcaggag
     9541 ttggctcatc tacttgaatg cgagcaaaac gtcgctctaa tgcagaatct ttttcgatat
     9601 atttttgata ttcatctagg gtcgttgctc cgatcgtttg taattctcct cgagctaacg
     9661 ccggtttcaa gatattcgaa gcatcaattg ctccttctgc tccacctgca ccaattaagg
     9721 tatgcaattc atcaatgaat aagattactt gaccatcatt atagatttcg tcgatgacct
     9781 ttttcattcg atcttcaaat tcaccgcgat atttcgtacc agcaaccaaa gatcccatat
     9841 caagcatcat caggcgcttt tcttgcatgt cttctggcac ttcacctaaa atgatttttt
     9901 gagctaaccc ttcagcaatg gctgttttgc ctacaccagg ttcacccacc agtacagggt
     9961 tattctttgt ccgtcggcta agaatctgga taagccgttt cacttctctc gaacgaccaa
    10021 caactgggtc cagcttgttt tcttttgcta atttcgtcaa atcacgtgct aatgaatcta
    10081 gcgtaggtgt tccttgcggt gtttgccgtt tatcttgtac atttacattt ctacgacgat
    10141 tcattccttt tccagcagaa tcaccagcta gccccatttt ttgtttcaga acttgacggg
    10201 cttttgaaag gctgatacct aagttgatca tgattcgagt agccatgata ctttcatctt
    10261 ttaataagct catcaaaata tgttctgttc caactttttg agaaccagtt ttcttcactt
    10321 cgctgctagc taaagcaaaa acttgtttca ttcttggtga atatggaaga aagactcctt
    10381 tagggtaaga acggatacca ccataaccaa tcagatgttc aatttcttca taaatttccg
    10441 tttcattcaa atccatttca cgtaatactt ttccagcaat tccattttgc tcgaccacta
    10501 aggctagtaa aacatgttct gaactaactg attgatgttt caaccgtttt gcttcttctt
    10561 gtgcaatttc taatactgct tgggctcttt cagtaaaaag ttcttccatc taatctcact
    10621 tcccatcttc ataacttaaa cgttccagaa atgctcggag aatccgagca cgtgtatgat
    10681 cttcatattc attgaaattc aatgtgtttt ttgcaattgc actcaacatc aagtttcctt
    10741 ctttttttgt aattatttgg tcttcataaa gtttttgaat aatagcaaaa gcatttttct
    10801 cactgattgt atcatcaaat aactgattta tctgctcaag taattgtttt ttatctgaaa
    10861 tccttacttt tgcaattcgt atataaccac caccaccacg tttgctttct actagataac
    10921 cacgttggat ggtaaaacgg gtattaatca catagttgat ttgtgaagga acacagttaa
    10981 agaggtctgc catttcaatc cttcgaatct cgatcatttc actattttcc agaattttct
    11041 ttatatatga ttcaattaaa tccgaggtat tctgatgtgc catcttattc attccctttg
    11101 actaatattg accttaatta taacgaattt gataatagaa agaaagaaga acgctttttg
    11161 ttaaataaat cctttagaat caacaaaaaa aaatacggaa tatactattc cgtatc
//
LOCUS       DACCXM010000089        11211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCXM010000089 DACCXM010000000
VERSION     DACCXM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.091"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            154..423
                     /gene="rpsN"
                     /locus_tag="IVY61_002031"
     CDS             154..423
                     /gene="rpsN"
                     /locus_tag="IVY61_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7971139.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            438..617
                     /gene="rpmF"
                     /locus_tag="IVY61_002032"
     CDS             438..617
                     /gene="rpmF"
                     /locus_tag="IVY61_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7971140.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            649..1026
                     /locus_tag="IVY61_002033"
     CDS             649..1026
                     /locus_tag="IVY61_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971141.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1047..1196
                     /gene="rpmG"
                     /locus_tag="IVY61_002034"
     CDS             1047..1196
                     /gene="rpmG"
                     /locus_tag="IVY61_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7971142.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1220..2203
                     /locus_tag="IVY61_002035"
     CDS             1220..2203
                     /locus_tag="IVY61_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7971143.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2233..2355
                     /locus_tag="IVY61_002036"
     CDS             2233..2355
                     /locus_tag="IVY61_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7971144.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2373..3896
                     /locus_tag="IVY61_002037"
     CDS             2373..3896
                     /locus_tag="IVY61_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7971145.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            3920..4159
                     /locus_tag="IVY61_002038"
     CDS             3920..4159
                     /locus_tag="IVY61_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971146.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4352..4813)
                     /locus_tag="IVY61_002039"
     CDS             complement(4352..4813)
                     /locus_tag="IVY61_002039"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7971147.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5008..5226)
                     /locus_tag="IVY61_002040"
     CDS             complement(5008..5226)
                     /locus_tag="IVY61_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7971148.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5344..6960
                     /locus_tag="IVY61_002041"
     CDS             5344..6960
                     /locus_tag="IVY61_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7971149.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7201..8541)
                     /locus_tag="IVY61_002042"
                     /pseudo
     CDS             complement(7201..8541)
                     /locus_tag="IVY61_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8817..8972)
                     /locus_tag="IVY61_002043"
     CDS             complement(8817..8972)
                     /locus_tag="IVY61_002043"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7971150.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9268..9600)
                     /locus_tag="IVY61_002044"
     CDS             complement(9268..9600)
                     /locus_tag="IVY61_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7971151.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10114..10305)
                     /locus_tag="IVY61_002045"
     CDS             complement(10114..10305)
                     /locus_tag="IVY61_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7971152.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10399..10785)
                     /locus_tag="IVY61_002046"
     CDS             complement(10399..10785)
                     /locus_tag="IVY61_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7971153.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3723 a         1775 c         2158 g         3555 t
ORIGIN      
        1 aattcgtttt caaatagtta tctattcata tgagttttat ttcatgttat ttattgttga
       61 caagagcaac tcttgttggt aaagtatcag tgtcaaaacg taacgattac gatttgaggt
      121 gtctaaatca aaataaaagg agtataaagg tatatggcaa aaaagtcgaa aattgctaaa
      181 gcaaagaaac aaatggcgat gattgaaaag tatgcagata aacgtcagga attaaaagcc
      241 gcaggagatc gcaccgctct cgctaaattg ccaagagatt caaatccgaa tcgtctgagg
      301 cttcgtgatc agacggatgg tcgtccaaga gggtatatgc gaaagttcgg tatgtcccgt
      361 attaagttta gagaacttgc ccatcaaggg ttgattcctg gagtaaaaaa agcaagttgg
      421 taaaaggagg aaatatcatg gcagtaccag caagaaaaac atctaaggcg aagaaacggt
      481 taagaagaac tcatcaaact gtagtaaaac cagaaatttc atttgacgaa gcgaatggtg
      541 attatagacg tagccatcat gtatctttaa aagggtacta taatggaaag aaagtaatta
      601 aaggagcttc aaaatagttt aatatataac ttttaggagg gaatcaaaat ggaaattatt
      661 tacccacctt tagttgaaga aggattaaag tattatcttg aaacgaccca gcaatcgcta
      721 gataaatgca ctttttatcg ttcgatggta gagagaggaa ttattactga aacgggttta
      781 ccgacccaac aggcaattga aaatggatta gtcaaagatt actatgagga ccaaggctta
      841 tcatttgatg attttttaag aatctatcca atttttgaag agtatgacga ggagttattt
      901 caatgtatcg atggttattg ggagatacct gttgatatga aagaaaattt agtttcacaa
      961 ttggaatctg gcgagttaac ttttgaagat gcacaacaaa ttcaagcata tcttgaagat
     1021 cgataagtat tgaaggagga agatttatgc gtcaaaatat tattttggaa tgtgtcgaaa
     1081 ctggagaacg tctgtatctc accagcaaaa ataaacggaa taaccctgaa cggttagaat
     1141 taaaaaagta ttcgccaaaa ttgcgcagac gggcaatttt taaagaagtg aaatagctaa
     1201 gtggtaaaaa ggtgacaaaa tgggcatacc agttacaatc gtttctgggt ttttaggggc
     1261 tggaaaaaca acgctaatca atcaagcctt gcaagtctca ccctacccca gggaagaaat
     1321 tattataatt gaaaatgagt ttggtgaagt tgggattgat cacaaattat tagttcatag
     1381 cgaggaacgg gtgttacaac taaataatgg atgtatgtgt tgcagtcttc gcagtgattt
     1441 attagcaata ttgatttctt tactggaaac aatagaagaa catcaacaac caatttcaca
     1501 aatcatcatt gaaactacag gtatagcaga tccccaacca atcattcaaa cacttttaac
     1561 agcgcctcaa ctaagaggaa gagtatatat cgatagttta ttgactgtta ttgatagtga
     1621 cagttttgaa cttattgaaa gagaaccaca agcccttaag caacttgtca tggcagatcg
     1681 catttttgtg tctagaaaac aaaacttgaa tttatctgca ctaaaaaaat tacagagaga
     1741 aatcaaaagt attaaccctt tatccgatat tcgaatgttc acacatagtg aaacgattca
     1801 aaaaaaggat ttttataatt tgaaaaaatt taatcatccc ttagtttttg aaagtgattc
     1861 atcagaaaat cactataatg gcaaatacta tcatcaacac catcaagtac atgagtttaa
     1921 atcaattctc ttaacttcag atcaagacat aaaaaaagat tttttactcc aatggattga
     1981 ctggctgatt ttaaccaacc aagggagcct gtatcgtttt aaaggattga ttgctttaca
     2041 agagcaggat tttatagttg ccatgcaaga ggtaaacgaa caggttaatt ttcaatacac
     2101 cactcgacaa aaagaccaaa ccactattat tctaatcgga aaaatgttgg atgaagaaag
     2161 gatcaaacag agttttgata aattactggc ggaaagcaaa taatgataag tagtgattgg
     2221 gggtaatttc taatggagaa aacggatata tctagtgcat atcgacgact taaaagcccg
     2281 aatattaaaa ctagaaaaag ggcattaaaa attatcaaag aacataaacg aaataaaatg
     2341 aagaaattag cataattaag gaaggaaaaa gaatgaagaa aatcagtgta ggacttattg
     2401 ggatagcagc tctaggatta ttgggagctt gctccagcac taatgatgca aaagtatcga
     2461 atgataaaga tggaaaatta gaaattgtaa caacattcta tccaatgtat gatttcacca
     2521 aaaacatcgt cggcgatgaa gcgaacgtgg atttaatggt tccagcggga tcagaacctc
     2581 atgactatga gccctcagct aaggatatgg caaaagctca tgatgctgat gtttttgtat
     2641 atcataacga aaacatggaa tcatgggtac caaaagctaa agaaagttgg aagaaagctg
     2701 ggcctaatgt agtagaagga acaaaagaca tgattttatt gccaggtagt gaagaagaag
     2761 atcatgatca cggagaagaa gatcaccatc atgaactaga tcctcatacg tgggtttcac
     2821 ccaaaatggc aattaaggaa gtttcaaata tcaaggatca gttagttaaa ttgtatccga
     2881 aaaaagcaaa agtatttgag acaaatgctg aaaagtattt gactaaactg aaacggttgg
     2941 atgctgacta taccactagt cttaaagaag cgaaacaaaa aagttttgtg acgcaacacg
     3001 cagcctttgg atatttagct ttggattatg gattgaaaca agttcctatt gctggactaa
     3061 gtcctgaaga agagccatct tctggtcgat tagctgaatt aaaagagtat gttaaaaaaa
     3121 ataagatcaa ttatatttac tttgaaaaga atgctaatga taaaattgca aaaactttgg
     3181 ccaacgaagc tgggataaaa ttggaagtac tgaatcctct tgaaagtctt acaaaagaac
     3241 aaatggataa tggtgaagat tatgtttcag taatggaaga caatctaaaa gcgttagaaa
     3301 aaacaactat ggttgctggt gaagaggtag ttccagaaaa agaagcgaag gatgaaaaga
     3361 cagttgctaa tgggtacttt aaagatgccg atgtgaagga tcgcgaattg agtgattata
     3421 caggcgaatg gcaatctgtt tatccgttgt taaaagatgg aattttggat gaagttttcg
     3481 attacaaagc aaaaatcaat aaagacatga cagctgccga gtacaaagat tattatacta
     3541 ctggctataa gacagatatt gatactatca acattaaaga caacaccatt gattttgtag
     3601 tcaacggtga acatcatcaa tacacataca aatatgttgg atacaaaatt ttgaattatg
     3661 aaaaaggaaa ccgtggtgtt cgttttaatt ttgaaactga tgatgcaggt gctggtcgtt
     3721 ttaaatatat tcaatttagt gatcatggaa ttgcaccaag taaggcagag catttccata
     3781 ttttctttgg tggagaaagt caagaaaaac tctataatga attgcataat tggcctactt
     3841 tctatccagc ttcattgtct aaacatgaaa ttgctcaaga aatgatggct cattaaaatt
     3901 caatttttgg aggaaataca tggtaagaaa agtattattg tctatggatt ccaagaatat
     3961 tgatgcttat cggcgttatc gatttgcaaa gcaaatgaga ttaaatggga gtacattagc
     4021 tgatattgct gcaactttac atgttagtat tgacacagtt catagagtat taaaacggga
     4081 tcctgattgt tgttttatca aggtcaatac tggaagaagt agacgtttag caaaacggca
     4141 taatagcttg gccaattaat tgataatacc agagtagatc atttttccct tatttggatt
     4201 tgatctgctt tttattatgt cttgaattgt caaatcaagc gcaacacttt atcaaagaag
     4261 acttcataag gtgttctcca atctaaacac tttctaggtc tgttattcag tttttcaatc
     4321 atctgattta tcgttgagtt atctaccgta gctatctctt tcgattttgg caagtattct
     4381 cgaatgagtc tattcgtatt ttaatttgtt cattgttgcc aagggcatga ggatctggaa
     4441 aataaaaggg catattattc aatgcttctg taactgcgaa atgattggag aattcttttc
     4501 ctctatctgg agtgatcgtt ttgaccgcct catttggaat actgcttaaa agttcaatca
     4561 ttttctcgct cacataagcg gctttctttc tggggacctt ctttcctgct aaaaggaagc
     4621 atgattttct gtctgtcaaa gtcactaaac aagtaccacc agttttgcca gctacaatat
     4681 cggctttcca atgaccaaaa tcacttctat catctgcgga ttttggacgg ccatggatcg
     4741 aatgagtaag gcaaatcttt ccacgagact cgacatgatt ttttgtatgt cttgtttttc
     4801 ctcgatggtg caatgaacgg atcaggcctt tattgccgtg tgagaggggt tctgtatcga
     4861 aaagatcatt gcaaattgca cgttagatcg tgataaaact aattgaaaag tccatatgtt
     4921 catgcttcaa acgattcgag atttcctctg gcgaccactg aaaatcgaca aataaatgac
     4981 gaacaattga ccaaactcac ggatcattta gaatgtgata acgtccgcat ttttttcttc
     5041 gataagcata atttttttcc gctttggaag gtgaatagtt tttttcttgt ttcttctcag
     5101 ttcatgtaaa atagtaaacg ggctatgctg aatgtcgttg ccaattatac taatagaaaa
     5161 tccttcttcg tgaagcagaa agattctttc tcgttcgagt atagtaagat gacgatactg
     5221 gctcataatt attttccttc taggagtgtt ctcgacaaac ttatactagt tggatatcga
     5281 ttatgactca tttttatgtg ttgcacttaa agtgtaaatt caaggtatag aaagaggttc
     5341 cctatgaaaa aaatacctat tacaatctta actggctatc ttggttcagg aaaaacgata
     5401 cttctaaatc atattctgta cggtgatcac ggaaagaaaa ttgctgtcat tgtaaatgac
     5461 ataggggaag taaatgttga tggggtatta gtcgacaaga aaggttttaa aagaactgga
     5521 gaaaaacttg tttcgatgcc gaatggatgc atttgttgta cattacgcga agatttgatt
     5581 gttgaactag atcaattagt gcaattttct gacttgaatc aaattgttat tgaagcttca
     5641 ggaatcagca aaccaattcc aattattcaa gcaattatat tagctaaaaa ccctttagac
     5701 attgatttga cccaacgatt agagattggt gccattatta tagtagtgga tgtaaaacgt
     5761 ttttgggata atttcgaaac gggtgaaaag attcaggaaa gaaaagctga aggtaatgta
     5821 gttgaagaag aacaggatat tcgtggcttg ttaattgatc aattcgagtg ttgtaacata
     5881 ttacttttaa ataatagtga cttagtgtca accaagacga tttacaaaat taacggtcta
     5941 attgctaagt tacagtccaa tgccaaaata attaggacaa ttaaaggtga agtagactta
     6001 tcgaaaattc taaatacaaa tctttttaat atagatgaga cttcctattc agtgggctgg
     6061 ttcaaagagt tagagcttgg ctatcaaaat catattcctg aaacagaaga atatggtatc
     6121 tcaagtttta tctatcgaaa tcggttactc cttgacgcac aaaaatttgc ccatcttacg
     6181 gataaaaatt ttcctggcaa tattacaaga gcaaaagggt ttctttggtt tgcccaacat
     6241 agtgattttt cgacgatctt tgaaacatcg ggtagctata aaagtattga atcattaaat
     6301 tattgggtag ctagtttacc taaaattgag tgtcaaatga ttatacaaaa aaatcccgaa
     6361 ttgaaggaag cctggcatcc gttgtttggt gaacgcatta ataaactagt ttttattggt
     6421 atgaagatgg atcaacaatg tattaaggaa caacttgagg gttgtttaac tgattctctt
     6481 aatgcagata atttccgaaa aatttcacca tttccatgga aaaagtctaa tatgtttagc
     6541 cagatatcgt taatgaaatt tttaagaaaa gctggtgtca agataggcga tgtcataatt
     6601 attggcattt tgattattac ctcttttatt cccttatttg tattctctat agaaaaggag
     6661 aatgaaacaa ctgaagatgc tatcaaatat gccatcgttc gaattgatgg caaggaaatg
     6721 gatcgcttcg atttggataa gatcaatcat aaactcgtga attatcatcc tgcgaaggga
     6781 caatataata ttgttgagat taaagatgga agaattcgag ttaaagaaga taatagtcct
     6841 gatcaaatcg cggttagaac tggatggatc tcaaaacccg gtcaaacgag tatttgttta
     6901 ccccataagt tagttattag tattgagaaa aaagaaagca cagattacta catttactaa
     6961 tctggaaata gatgaggagg tttttaataa gaaaaagata ttattattaa taatagcagg
     7021 ggctttatta agtgtgttaa gcgcttgtgg acaagaaaaa gaagcggtga caacaggcac
     7081 aagtatcttt aacgggacct gcaatggttt agttaacagt cggttagatt acttattttg
     7141 gctcttcgtc aatgagtgtt ggtgaagaaa ttcaatagaa taatcgagat actcacacgg
     7201 ctagacagct atcttgtcta gccgtgttgt tgttctggct ttagtcagtt gggtagtatt
     7261 cttagttttc ttaaaatatt ttcctcgata taaaaagatt cgttctcggt agttttgaaa
     7321 gtttctatag ccataggcaa ttcgttttag tactttgata tggttattca aacattctat
     7381 agggccgttg gaatagggaa cgcgcaacgc tcgtttgatt tctgtttggt atttcttgaa
     7441 ggtcgtgaac actggttggt agtgctctgg caactccttg tagtcttctt ttaggagcgc
     7501 gacgaattca ggaacatctt gtcttctgat aacggaaaga aaatcctggt agacttcgta
     7561 gcctcgcttt aaggaatcat cataagataa tagccgatcg actatctctg cttctgtcag
     7621 atgatctcga aatgacggac gccagtagcg atgctgacct ttcaattccc acgcattctt
     7681 ttgaagtagt ttccagtatt tctttaagtg cttcgaccga cgatcccctt ttttaaaggt
     7741 cttcattact tgaattcggt ggttggtaaa agcccgacca atgtgttggc taatatgaaa
     7801 gcgatcggcg acgattcggg cattcggaaa acactctttc accaatgtgc gatagggtgt
     7861 atagaaatct gaaaccacta gacgcacttg ttcacggact tttatagggt agcgcaagaa
     7921 atagtcgcgt aaaggattga gttgccgatt ttccacaata tcaatcagtt gatgggtctt
     7981 gccgtccatc atgacaaagc tcatggctcc cgagacattt ttcactgatt tgaattcatc
     8041 gaaacacacc acttctggta acagctggct caaagaaatg gttttaggcc gaaggctgcg
     8101 aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca aagacataga
     8161 aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa tggagcagcg
     8221 acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac atttgaagcg
     8281 ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata agatggatac
     8341 ttttttccag ccctatttaa tgatcgtttg ttcattttga tagtggcgat gaaagcattc
     8401 ctccggttga taggtcaatg tgccatcaaa aacaaaacat gtttcgtcat tgattcgctc
     8461 tttgcgaaag gcatcttcat gaaagataag atttaggtct agaatatcta gtaactcttt
     8521 ggtaaactct gaataggaca tcttgaaaca ctcctttgaa tggttttcgt cgactttatt
     8581 ctaaaggatt ttaaggtgtc tgtttgcgtt tattagaaaa aagaaaaagt attggcaaca
     8641 gaaggcgtca ccaacactag atattataga tcccttattt tcttcgcttc aaattgtcaa
     8701 ggagtcatca taaattttgt atggattctt ttgtttgggt agtagtcttc taaataatct
     8761 tggattaaca ccttcatctc tattttactt ttatatttgt tggagtcgat taattctcat
     8821 ttgattgatc gatgaaacgc ttcaattact gcattgtcaa caggagtccc agtttggctc
     8881 actgaatgtg tgatgtggtg gtcgttcaac agctcttgat aaataaaaga ccgatgctgg
     8941 ctacctctat cggaatgaat taccgtaggc aattctcctt aataaatttg taatggacgg
     9001 acgacaatct cttttttcat gtgtgaatct accatatagt aacgtagaat acgagttgct
     9061 aagtcaatat aggtggacaa atagaggcga ccgtcattac atggaatgta agtaatatcc
     9121 atcacccaaa cagcatcaat ttggttctgt tcaaattatc gattcaacat ttttttacca
     9181 tttctttgat tgttttttct ctaccatgag aagatttgcg tcgatgaaat cctcttgtgc
     9241 ataaattcat ttcacgcata agaacagcta caacacgttt attgatttcg attccttcat
     9301 cgtaaagttt ttgagtgatc cctggagatc cgtatcgatc tttgtgttcg tagaacactc
     9361 gtcttcctct tttagtcgtt tattttcctg ttcaagaatg tagaaatctg ctttatcagg
     9421 taatcttttt ccttgtccag caaaagcatt tttcccgtat cgacgatatt ctgaaaccca
     9481 tcgggaaatt gttggacgag agatattgta ttcttcagca attttcttgg tagttacttt
     9541 tttctcaagg atatcctgtg aaatctttag tttaaattta cgatcatatg tttttctcaa
     9601 tagagttcat tccttccggt aacgtaactg atagtcaaga aaagtgtatc atagcccaac
     9661 ttagtatcag ggtttgtcaa ctaaactgtg gaagttgata gataagagtt tttaacaacc
     9721 aaaattctct tgactatttt tgaaatcaga caaattttga ttggacacat agttgaataa
     9781 atctaccgtc gttacggaag gtaaatcgca tgcttttcaa ttctagagga gaagtcctcg
     9841 ttgaagtacc atagcccagt tctggattcg acctcctgcc cacttcatac gtaaataaag
     9901 taattttagg agtgagactg tgacaaaagt tatttttaag ctaaaaacga attaaaaaac
     9961 gttgataatt aaaatcattc gttttagatt ctatgaatca ttatagaacg ctgtaaaatc
    10021 gatactttat aattataaag aagatagttt caaatttatt gttcgaaatt tgaaactatc
    10081 tttttactta tgtcacagac tcccttagtt gagttattct ttaatttcga atttcgagat
    10141 agctagatat attgcaagca tacattcttg ataacaatct tgatcatatt ttccagttct
    10201 ccaggattgc ttatgaatca aagagctaaa tcttttgaga attgcctcaa cagcttcttc
    10261 atttccagcc tttgctaaaa tgaaatcttc ttttaaagaa ttcataaatc taccttcttc
    10321 ccatggactt ataatctatt tctttgggaa ggaggagaac gagcaaagaa gcatacaaaa
    10381 ttagttatta tgcagatatt aaaccagcat tttttgaatt tttttgtaaa gtcgatgttt
    10441 taaatttgtc gcaccctgtc ttgtaatccc cagaaccttt ccaatttctt tatcagtttt
    10501 atcaaagata aatttttcga taagaaaaat ttgttcaggt tctttcaaca aaggaaggat
    10561 ttttgctaat tttatatctt caacaaggag agtcacattt aaaacattaa tcggttgttt
    10621 aaattttata gtatctttag taaacaaata tggtgggagt tgatcaagtt gttcatgttt
    10681 ttgaatttct tcttgttttt tataataagt attagcggtg ttcaaaatga ccttttcaaa
    10741 gaatcgaatc aactgggcat ttacgcgttc gtcgttatct gacatattgc acacctcctc
    10801 cattaagatt taaagggaaa agaggcaaaa aacacgaaaa gaagctaatg caattatgct
    10861 gcgctgcgaa taacttctat aagctcacat gcattgatag gactgctgat gtttattttc
    10921 aaaaatgttt ttttagttga taatggatag ttatctttat acttagaaaa gaaaattcat
    10981 atatcttctt aaatatcaaa catgtcctta tggctaagaa aatgtcttga gctttttacg
    11041 tataataaat cttttctaaa acctcctttt ggataatatt cttattcgtg tatagattgt
    11101 attttttatt tcttccccct tcataattaa caactagaaa agagttcgaa tagacacgta
    11161 tttgaacgaa aatttttatt ggattcccaa attggaatat ttagaaacga a
//
LOCUS       DACCXM010000090        11199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCXM010000090 DACCXM010000000
VERSION     DACCXM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.092"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            161..1420
                     /gene="lysA"
                     /locus_tag="IVY61_002047"
     CDS             161..1420
                     /gene="lysA"
                     /locus_tag="IVY61_002047"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP7971154.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1407..2207
                     /gene="dapF"
                     /locus_tag="IVY61_002048"
     CDS             1407..2207
                     /gene="dapF"
                     /locus_tag="IVY61_002048"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP7971155.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2210..3091
                     /locus_tag="IVY61_002049"
     CDS             2210..3091
                     /locus_tag="IVY61_002049"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP7971156.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3088..3804
                     /locus_tag="IVY61_002050"
     CDS             3088..3804
                     /locus_tag="IVY61_002050"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP7971157.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3810..4505
                     /gene="dapD"
                     /locus_tag="IVY61_002051"
     CDS             3810..4505
                     /gene="dapD"
                     /locus_tag="IVY61_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP7971158.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4521..5522
                     /locus_tag="IVY61_002052"
     CDS             4521..5522
                     /locus_tag="IVY61_002052"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP7971159.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5519..6880
                     /locus_tag="IVY61_002053"
     CDS             5519..6880
                     /locus_tag="IVY61_002053"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP7971160.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6840..7991
                     /locus_tag="IVY61_002054"
     CDS             6840..7991
                     /locus_tag="IVY61_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP7971161.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7966..9135
                     /locus_tag="IVY61_002055"
     CDS             7966..9135
                     /locus_tag="IVY61_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7971162.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9310..10689
                     /locus_tag="IVY61_002056"
     CDS             9310..10689
                     /locus_tag="IVY61_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7971163.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10970..11199
                     /locus_tag="IVY61_002057"
                     /pseudo
     CDS             10970..11199
                     /locus_tag="IVY61_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3730 a         1952 c         2498 g         3019 t
ORIGIN      
        1 ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat atactagaca
       61 attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc aaatgaaatt
      121 ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat tgataattgg
      181 cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg tgtatgatga
      241 agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga aacgttttga
      301 tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc atttagttcg
      361 tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg ctttgcaagc
      421 tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag aggaactgat
      481 ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg aagttgaatt
      541 attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt
      601 cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt caaaattcgg
      661 catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag aaagcagcta
      721 tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg ttactgcttg
      781 gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac ctctttcctt
      841 gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga tcccagtcga
      901 agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac gaaaactgac
      961 cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg gtacaacact
     1021 atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg tagatggcgg
     1081 gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg atttagcagc
     1141 aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg
     1201 cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt
     1261 ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag cgacacgacc
     1321 agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac aagcattttc
     1381 tgatctacta aggggggaag tttcctatga tccttcataa atataatggc tgcggaaatg
     1441 attttatctt attagattac gcagaagaca cagattatag tcaattagcc gcttctttat
     1501 gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat ccgttggaaa
     1561 tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt
     1621 ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac gtagagacat
     1681 tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc agcgtagata
     1741 tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa
     1801 accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt ttaggaacga
     1861 ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac
     1921 tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta gaagtagtca
     1981 atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca cttgcttgcg
     2041 gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc
     2101 aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat gcgatacaaa
     2161 tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga tgataaaagg
     2221 ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt atgctggttt
     2281 ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag ttttaggtac
     2341 aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc aactgacagt
     2401 aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata atacgaaaga
     2461 aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt tggtcatcac
     2521 cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag caattgcaga
     2581 agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca tgtcgattcc
     2641 aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga aagaagcctc
     2701 aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat tcttccttta
     2761 cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg gtgtaataag
     2821 tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg
     2881 aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc atgccttgtt
     2941 cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact taccttccgg
     3001 acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat tagctcagct
     3061 tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag tggacagatg
     3121 ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct tttacggacg
     3181 gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct tattgatttt
     3241 tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca acttccgtta
     3301 gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga acatgccaaa
     3361 catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat gaatcaacta
     3421 atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga aaaacatcat
     3481 agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac aatccaagaa
     3541 gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga aaaccaaatt
     3601 ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt gtttgctgct
     3661 actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt tgctgcaggc
     3721 gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa attagaagat
     3781 ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc cgctacattc
     3841 aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat
     3901 ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga
     3961 cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg
     4021 ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct cgtatcgaac
     4081 caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag
     4141 cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg ggtgctgtct
     4201 taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct gtcttagcag
     4261 gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg
     4321 cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag
     4381 cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat
     4441 taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg ttacgcgaat
     4501 tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg gcaaccggat
     4561 tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta ggacgattga
     4621 aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa aagaaacaca
     4681 ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta ttttctgcag
     4741 gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta tttgtcatag
     4801 ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca gaggtcaatc
     4861 tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc attcaagcgg
     4921 tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat tattcgacct
     4981 atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg
     5041 acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg gaaatcgatc
     5101 agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag acccgtaaga
     5161 ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg
     5221 gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa gaaattagag
     5281 agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca cataaatatc
     5341 cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc agaaaagaca
     5401 cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc aaaggagccg
     5461 ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag gtggcaagat
     5521 gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg aagagcaatt
     5581 caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc
     5641 tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt tgatttatgc
     5701 ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg atcgtttcat
     5761 tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag cacgcataca
     5821 gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag gtgaatatct
     5881 gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa aagacctgat
     5941 tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc
     6001 atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc ctgcaggaaa
     6061 agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg cgaagatgtt
     6121 gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg ctgatccgcg
     6181 aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga gagagatggc
     6241 ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag aggctgatat
     6301 ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa tatcgaatca
     6361 cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt ttctttccat
     6421 cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag caatgagcat
     6481 ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca atatcggagt
     6541 gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag aattaaaaga
     6601 aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg
     6661 gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat tgaacaaatt
     6721 agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga tcatcggtgt
     6781 accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg tgaatacgga
     6841 tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt catgaattca
     6901 agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc catgaaatcg
     6961 gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt gcttttcgaa
     7021 cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct tctaagcatg
     7081 aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta gggttgactg
     7141 actttataac taaacacatt gaggatctta tttataatgt agtactgatc tatcagccat
     7201 cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg gataaatatg
     7261 aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga gaagttttct
     7321 ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt aaaggccagt
     7381 ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt
     7441 tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat ctaatgaaat
     7501 ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt attgtatccg
     7561 gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga ttagaccatt
     7621 tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac ggctatccag
     7681 ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa ctacatgaat
     7741 tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa tatccatgtg
     7801 tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg ttcgattttg
     7861 atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt acagaaaatc
     7921 ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat gaatttatcc
     7981 aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca attcgatcag
     8041 caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact
     8101 cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc tcactatacc
     8161 ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca acagcgttat
     8221 ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt
     8281 gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc agcgcctgct
     8341 tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc gatagatact
     8401 tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga acaatatgga
     8461 tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac gacattgtct
     8521 gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt tgttataagt
     8581 gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc tacttatctt
     8641 ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac tggttggcga
     8701 gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt ccatcaatat
     8761 ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg
     8821 gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta tctggtccag
     8881 caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta cttgttttgc
     8941 aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt agcggaacag
     9001 gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg ttatatccgg
     9061 atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat caaagacttt
     9121 ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt tgcggtatat
     9181 tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc ttttgattca
     9241 taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat attcaaggag
     9301 gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg agggcatcaa
     9361 tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct atttgaagca
     9421 ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga caaggtaaca
     9481 gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa tgttcatatt
     9541 ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac ggttactagc
     9601 gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag ttctggtgtc
     9661 aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt gatgcgtgga
     9721 tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct
     9781 gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa agctgggaaa
     9841 gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga tgctgagatg
     9901 acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac aaatgcaaaa
     9961 atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc
    10021 ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg gttaaaaggg
    10081 atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca aacgaatctt
    10141 ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc acgcaaaaaa
    10201 atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt aaaacatctt
    10261 tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc attaagtgtc
    10321 tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa agcaggagta
    10381 gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc agaagaacaa
    10441 ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca attgctagga
    10501 ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc actagcgatc
    10561 caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca gccaggcttt
    10621 gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga agaaccattt
    10681 gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct tgcatcaagt
    10741 aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg acttattacc
    10801 gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac acgatttgtg
    10861 taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta ttttttatgg
    10921 ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat ggcaaaggcg
    10981 attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa ttcgggagaa
    11041 gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg gtttctctat
    11101 caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa cggcatacac
    11161 gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACCXM010000091        10861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCXM010000091 DACCXM010000000
VERSION     DACCXM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.093"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            12..84
                     /locus_tag="IVY61_002058"
     tRNA            12..84
                     /locus_tag="IVY61_002058"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="IVY61_002059"
     tRNA            88..160
                     /locus_tag="IVY61_002059"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="IVY61_002060"
     tRNA            168..249
                     /locus_tag="IVY61_002060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="IVY61_002061"
     tRNA            267..339
                     /locus_tag="IVY61_002061"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="IVY61_002062"
     tRNA            351..422
                     /locus_tag="IVY61_002062"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="IVY61_002063"
     tRNA            439..524
                     /locus_tag="IVY61_002063"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="IVY61_002064"
     tRNA            535..608
                     /locus_tag="IVY61_002064"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="IVY61_002065"
     tRNA            618..691
                     /locus_tag="IVY61_002065"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="IVY61_002066"
     tRNA            711..783
                     /locus_tag="IVY61_002066"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="IVY61_002067"
     tRNA            811..884
                     /locus_tag="IVY61_002067"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="IVY61_002068"
     tRNA            898..971
                     /locus_tag="IVY61_002068"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="IVY61_002069"
     tRNA            995..1084
                     /locus_tag="IVY61_002069"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="IVY61_002070"
     tRNA            1097..1170
                     /locus_tag="IVY61_002070"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="IVY61_002071"
     tRNA            1189..1264
                     /locus_tag="IVY61_002071"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="IVY61_002072"
     tRNA            1266..1336
                     /locus_tag="IVY61_002072"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="IVY61_002073"
     tRNA            1350..1423
                     /locus_tag="IVY61_002073"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="IVY61_002074"
     tRNA            1448..1535
                     /locus_tag="IVY61_002074"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="IVY61_002075"
     tRNA            1557..1628
                     /locus_tag="IVY61_002075"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="IVY61_002076"
     tRNA            1651..1740
                     /locus_tag="IVY61_002076"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="IVY61_002077"
     tRNA            1753..1826
                     /locus_tag="IVY61_002077"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="IVY61_002078"
     tRNA            1845..1917
                     /locus_tag="IVY61_002078"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="IVY61_002079"
     tRNA            1921..1993
                     /locus_tag="IVY61_002079"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="IVY61_002080"
     tRNA            1998..2068
                     /locus_tag="IVY61_002080"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="IVY61_002081"
     tRNA            2081..2154
                     /locus_tag="IVY61_002081"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="IVY61_002082"
     tRNA            2166..2237
                     /locus_tag="IVY61_002082"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     gene            2503..3696
                     /locus_tag="IVY61_002083"
     CDS             2503..3696
                     /locus_tag="IVY61_002083"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP7971164.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="IVY61_002084"
     CDS             3826..5298
                     /locus_tag="IVY61_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7971165.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="IVY61_002085"
     CDS             5381..6169
                     /locus_tag="IVY61_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP7971166.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="IVY61_002086"
     CDS             complement(6236..6904)
                     /locus_tag="IVY61_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7971167.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="IVY61_002087"
     CDS             7019..8731
                     /locus_tag="IVY61_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7971168.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="IVY61_002088"
     CDS             8734..10506
                     /locus_tag="IVY61_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7971169.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>10861)
                     /locus_tag="IVY61_002089"
     CDS             complement(10588..>10861)
                     /locus_tag="IVY61_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7971170.1"
                     /translation="FISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLL
                     TELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3117 a         1960 c         2504 g         3280 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 a
//
LOCUS       DACCXM010000092        10524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCXM010000092 DACCXM010000000
VERSION     DACCXM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.094"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(176..661)
                     /locus_tag="IVY61_002090"
     CDS             complement(176..661)
                     /locus_tag="IVY61_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP7971171.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1141..1782
                     /locus_tag="IVY61_002091"
     CDS             1141..1782
                     /locus_tag="IVY61_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7971172.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1801..2436
                     /locus_tag="IVY61_002092"
     CDS             1801..2436
                     /locus_tag="IVY61_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7971173.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2454..3269
                     /locus_tag="IVY61_002093"
     CDS             2454..3269
                     /locus_tag="IVY61_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7971174.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3588..5024
                     /locus_tag="IVY61_002094"
     CDS             3588..5024
                     /locus_tag="IVY61_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7971175.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5213..7645
                     /locus_tag="IVY61_002095"
     CDS             5213..7645
                     /locus_tag="IVY61_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7971176.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            7875..8942
                     /gene="rlmN"
                     /locus_tag="IVY61_002096"
     CDS             7875..8942
                     /gene="rlmN"
                     /locus_tag="IVY61_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7971177.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9135..10238)
                     /locus_tag="IVY61_002097"
     CDS             complement(9135..10238)
                     /locus_tag="IVY61_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7971178.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3473 a         1800 c         2282 g         2969 t
ORIGIN      
        1 ttttcgtaga atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt
       61 tcagcgttca tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt
      121 gtgctaaata aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca
      181 aattttcata aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat
      241 ccataatcaa cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa
      301 tagcagcatc gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag
      361 tcactgtacc tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata
      421 agcgcttttc ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca
      481 agatgacaag atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg
      541 gaccaggtag ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt
      601 gaaaagagat acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca
      661 tcgcgcatca ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa
      721 taatcatgta aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt
      781 aggtgttttt agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat
      841 cttggagtcg attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg
      901 gtcgttgcta gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg
      961 gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa
     1021 cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt
     1081 ataatacgtc ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag
     1141 atggagtatt tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa
     1201 ttatttttat ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg
     1261 tctttgcatt ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga
     1321 acaccgctgt tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt
     1381 tttgatcgtt tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca
     1441 gagatctttc gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt
     1501 ttgcaattaa gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt
     1561 gttttacctt ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat
     1621 atattaggtt taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact
     1681 ttactgcctt tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc
     1741 gtaatgaaac aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat
     1801 atgattgaat tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg
     1861 aatctaatca ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa
     1921 accacgcttt tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta
     1981 gacggtgtac cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta
     2041 ttccaagact tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca
     2101 aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct
     2161 tcattaggga ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa
     2221 cagcgggtag cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa
     2281 ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg
     2341 aaagaagatg gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc
     2401 ggtgatcaat tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa
     2461 aaaccagttt tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca
     2521 aaaaaacttc agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt
     2581 tagacgatac atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg
     2641 atattgattt agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca
     2701 ttgactggtc aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg
     2761 gctattccaa aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa
     2821 acgatcaagt gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata
     2881 ataagagatt aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag
     2941 aagtattaaa agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag
     3001 cattgatcga tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag
     3061 actattattt gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg
     3121 agagtgagga ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca
     3181 atgaagccct agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt
     3241 ttggcgaaga tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca
     3301 cagtcccttt ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa
     3361 tggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc
     3421 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta
     3481 agcgactttt tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt
     3541 tattctaaaa gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag
     3601 aacttacctc atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag
     3661 aaggagcttg gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac
     3721 ctggaaagac atttaaagga acgaacggtg atttggcagt agaccactat catcgttata
     3781 aagaagatat tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt
     3841 ggacacggat ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg
     3901 agagattgat cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt
     3961 gggatcttcc acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg
     4021 aagatttcac tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact
     4081 ggattagttt gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc
     4141 cgccaggtgt gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa
     4201 atgcatctgt catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct
     4261 ttgcgtatac gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa
     4321 atgcagaaga cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga
     4381 ttgctgccat gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg
     4441 cagaattgct agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga
     4501 cgaatgcatt caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa
     4561 aaagttcatc agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg
     4621 tcgaacgaac aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc
     4681 gtatcacaag ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg
     4741 acaagttgac agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc
     4801 acgtcaaagc catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct
     4861 ggtcttatac agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat
     4921 atgttgatca agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt
     4981 actggtacca aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac
     5041 aagaaggtac ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga
     5101 aaaactgtaa aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta
     5161 gttcccttac ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca
     5221 aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca
     5281 gcaggaacaa atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg
     5341 ctttttaaaa ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg
     5401 aaatacttac caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa
     5461 gaatacagag ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt
     5521 cggtatctta ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa
     5581 tatcatgggg acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa
     5641 taacgttggt ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc
     5701 acagcctgga agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag
     5761 taatctggaa ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga
     5821 attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat
     5881 gaaacagcgc gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaata
     5941 tgagaagaaa aaagcggtga gccagcaaag ggaaaatact gctgatgaat ggcaggaacc
     6001 agaaaataca gagccggagc agctaagttt tgttccaatc gacagttttc aagaaaatat
     6061 ccatcctgcg aatctagaga aaccagtacc agatacacca aaacagacca atacagcaga
     6121 aggtttttca gatgagttgc cagaggatga cggtacctca ttagaatttg agattgaagc
     6181 agaacaggaa aatcaagact atgaattacc gacagttgat ttgcttgatt ctataccaac
     6241 tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac
     6301 tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac catccgtcac
     6361 aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga acttaacaga
     6421 tgatatcgcg cttgctttag cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa
     6481 atcactgatt ggtattgaag taccaaatgg aaagatcagt atggtttcct ttagagaaat
     6541 catcgaagca caaccaaacc acccagataa attactagaa gtacctttag gacgtgatgt
     6601 ttctggacgg gtacaaacgg ctgatctttc gaagatgcct cacttactcg tggctggttc
     6661 gactggtagt gggaagtcag tagcaattaa tgggattatc acaagtatat taatgcgtgc
     6721 taagccccat gaagtaaaac tgatgatgat cgatccgaaa atggttgaat tgaatatgta
     6781 caacggcata cctcatctgt tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc
     6841 cttacaaaaa gtagtacaag aaatggaaga acgatatgaa aaattcgcgg caaccggagt
     6901 aagaaatatt tctggttaca atgaatttgt tcaacagaaa aacttggaaa acggaacgaa
     6961 acatccaacc ttaccattca tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt
     7021 agccagcaat gaagttgaag atgcgattat tcgtttagcg caaatggctc gggcagcggg
     7081 gattcatatg atcctagcta ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa
     7141 agcaaatgta ccttccagaa tggcatttgc ggtatcaagc ggtacagatt ctcggacgat
     7201 cattgattct aatggggcag aaaagctctt agggcgagga gatatgctat tcttgccaat
     7261 gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg aagtagaacg
     7321 ggtcgttcaa tttgttacag atcagcaaga agctcattat gaagagaaaa tgatgccgac
     7381 agatgaagtt gaaacgacag gtgctccaga acagccacaa gatgagctgt ttgaagaagc
     7441 aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa gaagatttcg
     7501 gatcggttac aaccgagcag cacgtttagt agatgaactg gaagctcatg gagttgttgg
     7561 cccgtcagaa ggaagcaagc ctcggaaagt attcattgag caggaagaaa catcagaaac
     7621 gttgccggac gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc
     7681 aataaaaaga caaggcatga tgataccaaa agaaaaaatg gtttttttcc atcttttcta
     7741 atacggtatg atgtcttgtc tttttagttt ttgtgagatt aaagttctat tcgatttatt
     7801 tgtgaagtat tgcattaagg aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa
     7861 agagggaaaa tactttggaa aaacagtcca tttatggctt gacgaatgaa gaattaatca
     7921 attggtttat agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt
     7981 atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa
     8041 aattatcgga gcattttatc atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg
     8101 atggtacagt aaaatatttg tttgaactcc ctgataaaaa catgattgag actgtactga
     8161 tgagacaaga atatggcttg tcagtttgtg tgacgacaca agtaggatgt aatatcggct
     8221 gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct ggagaaattg
     8281 ttgcacagat catgatggtc caacattatt ttgatgagcg caatcttggc gaacgcgttt
     8341 cacatgtcgt tgtaatgggg atcggtgagc catttgataa ttacgacaat gtaatggact
     8401 ttttgcatat catcaatgat gccaaaggat tggcaattgg tgctcggcac attacagttt
     8461 ccactagtgg attagcacac aaaatcaagg aattcgctaa taacggcttg caagtcaatc
     8521 tggctatttc cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg cggatcaacc
     8581 gaagtttccc aattgagaaa ttgatggaag ctgtggatga gtatttagag aaaacaaacc
     8641 gtcgaatcac gtttgaatat atcatgttga atcaagtaaa tgatagacca gagcatgcgc
     8701 aacaattagc agatctatta aaagataaga aaaaattagc atatgtcaac ttgattccat
     8761 ataatccagt cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat gtattgagat
     8821 tttatgatgt attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa catggtacag
     8881 atattgatgc agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat
     8941 aatcgtatcc tactcgtttt ttcaattacc ataaaaggag cagttttctt aattaaggaa
     9001 ctgttcaact taaataagag aaaagattct atagcgaaag ctataaagct ttttttgaca
     9061 agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga tgacagcctg cttgatgggg
     9121 gttttagcaa ttatttaact gattttctta tatccttgct ggctttcttg tgtagcatga
     9181 taagtttcta cgtcaagaat ggcctgttta aatttttctc gggtgatttc ataaggcaca
     9241 tgtttaatat catttgttgc catggcttta tctaagattg cttctaaatc ggtcgtgaca
     9301 ttgagattca tgtcggttaa cgatactgga agttggttct ttttcataaa cacatagatt
     9361 ttatcaaaca aatcgaactg ctgatccatc atcaataagg tcagtacacc ataactgaca
     9421 gatgctccgt gaaggtatcc ctccattttt tctagaacag tcgtaccgta atagaatgag
     9481 tgtgccatag cagaattata gttattgtta actaaaacag aaaccaatcc ggtagtaaca
     9541 atgatatccg aaacgacttg ttcaattgca tatgagggac gatcgtcggc actatctttc
     9601 aatgcttgcg taccataagt caagagacga tcaatgcaca tttttccgat ttggattcct
     9661 aatctatccg tgtagcctag atcatcgcca cgagcagaaa aagtagattc gacttctttg
     9721 gataaagcat cgccaatacc tgcccataag tatttatcag gtgcttcggc aataattgct
     9781 gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc cattacgcct
     9841 tcttcgttat agatgacaca taccgcagtg acgggcgaac agttcgaacc aatagtggga
     9901 aaggtgaaga ctggtttagc tagtttttca ccaatgactt ttacagtatc gcaagcacgg
     9961 ccaccaccga ctgcaaacag gacgtctgct ttttggacac tttcatgctg gatcaactgt
    10021 tcaatatttc cataagttga attacccccg taccagagac tggcgacaac ctctatttcc
    10081 tgtgtcaaag ctacttttat tttgttttct gcctttgaaa gtgcagtctt ccctccaatc
    10141 agcactactt ttgtcccata ttctttgacg attccatttg ctttttcata cgcatctgtt
    10201 ccaatggtat aatttggtaa gaaaacactt tgactcatat tcatttcctt ctttcttcgt
    10261 tgaaacgggt taaaatttta aagagtgatc gatctgactg cggctgtaga tggagtagag
    10321 gtgaacctaa gacaggataa gtgatgaata aggtggcatg aacaccttga aatcatcaat
    10381 aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt
    10441 cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa
    10501 ttaagattat tatatgaaaa cagg
//
LOCUS       DACCXM010000093        10212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCXM010000093 DACCXM010000000
VERSION     DACCXM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.095"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            127..276
                     /locus_tag="IVY61_002098"
     CDS             127..276
                     /locus_tag="IVY61_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP7971179.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            287..1801
                     /gene="dltA"
                     /locus_tag="IVY61_002099"
     CDS             287..1801
                     /gene="dltA"
                     /locus_tag="IVY61_002099"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP7971180.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1798..3003
                     /gene="dltB"
                     /locus_tag="IVY61_002100"
     CDS             1798..3003
                     /gene="dltB"
                     /locus_tag="IVY61_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP7971181.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3048..3281
                     /gene="dltC"
                     /locus_tag="IVY61_002101"
     CDS             3048..3281
                     /gene="dltC"
                     /locus_tag="IVY61_002101"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP7971182.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3284..4552
                     /gene="dltD"
                     /locus_tag="IVY61_002102"
     CDS             3284..4552
                     /gene="dltD"
                     /locus_tag="IVY61_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7971183.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4693..5775
                     /locus_tag="IVY61_002103"
     CDS             4693..5775
                     /locus_tag="IVY61_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP7971184.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5775..6578
                     /locus_tag="IVY61_002104"
     CDS             5775..6578
                     /locus_tag="IVY61_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7971185.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6648..7388
                     /locus_tag="IVY61_002105"
     CDS             6648..7388
                     /locus_tag="IVY61_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7971186.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7399..8403
                     /locus_tag="IVY61_002106"
     CDS             7399..8403
                     /locus_tag="IVY61_002106"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP7971187.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8448..10211)
                     /locus_tag="IVY61_002107"
     CDS             complement(8448..10211)
                     /locus_tag="IVY61_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971188.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3273 a         1695 c         2072 g         3172 t
ORIGIN      
        1 tgctattttt ataaaagcta tgtgacaaaa ctgttagtaa aaaaacggtt tctgtaagct
       61 agacacatgt ttctttttgt caaaattagt tacactttgt gtgaggttat atgaggaggg
      121 aatccaatga acaaatggcg ttctaatcca aaattgaatt attggggaaa attcatcatt
      181 cgaacaattt tttatagtgc agtactgctg ttcctgattt atctgtatca ctataaaaat
      241 attcaaggag gaacttttat ctataacgaa ttttaggagg aagagtatgg aaatcaaaac
      301 gattattgaa gcaatcgatg attggggaat aaatgaacct gatcgagttg cttatcaggc
      361 ggatgagacg catacttttg gtgaattgaa acaagcatca gacgcacttg cttattattt
      421 agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt ggcaatttgg agtttgaaat
      481 gattgtctca tttctagggg ttgtcaaatc tggccatgcc tatttgccga ttgaagagca
      541 tacacccaag gagcgtatcc tatcgatctt tcgggtggct aaaccatcta tggtcatcag
      601 tatcggggac tggatagaag atattccttc ggttcctgtc ataacaaaaa aagaatttca
      661 agaaatcaag aagataccag taggatttta tcccagaaat agcgtaaaag gtacagaaaa
      721 tttttatatt atttttactt ctggtactac tggagaacca aaaggcgtac aaatcagtca
      781 tgataatcta gtcagctttg ttcaatggac gatggaagac tttgggatca agcctggtat
      841 gcatttctta gcccaagcgc cattttcttt tgatttatcg gtattttcta tttatcctgc
      901 attagtttct ggcggaatgc tgaagccgtt aaataaagca gttgttcaag actttcgtca
      961 attattcgcg acattgccag ccttgaagtt gaatgtgtgg gtttctactc catcatttat
     1021 ggatatttgc ttgatggaac ctacattcaa tggagaaaat gtgccggaat tggacatgtt
     1081 tttattttgc ggagaagaat tgacgaaaaa aactgcagaa agtctacttg atcgattccc
     1141 aaatgcacgg atatataata cttacggtcc aacagaagca actgtagcta tttcaagtat
     1201 tcagattgac cagcatgtac tagatagtta tgaccgacta ccgattggct acgtaaaaaa
     1261 agacacgaaa gtttctattg tccaagaagg taaacctgtt tcaaaaggag aaacgggtga
     1321 aatcattatt gcaggaccaa gcgtatcaaa aggttacctg aacaatcccg aaaagacagc
     1381 tgctgcgttc ttttgtcaaa atgacttagc aagttaccat acaggggatg caggtagact
     1441 agatgaatat gggcttttat tttatgaagg acgtatggat cagcaggtca aattacatgg
     1501 ataccggatt gaactcgggg atatcgaaca ttatctgctc caagacaacc gaatcaaaca
     1561 agctgtcgtc gtaccaaaat accaaggaac aaaagtacag cagctggttg cttttgtcgt
     1621 attagaaaac aaaacgaaac agccggattt tcagttgact aaatctatca aagaacaatt
     1681 actagaagtt gtaatggatt atatgattcc tcagaagttc cattatgtcg atctgctgcc
     1741 tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca gctgaggtga atcctgaatg
     1801 atgttacctc atatgatccc ttatgcagag ccgttttatt tttttctttt agccattgcg
     1861 cttgtaccga tcgttctatc tttattgata tgggaaaaaa gattaccagt ttatcaatca
     1921 gcagtgacac ttttcttttt atttgtcagc tttggaggta attattggca acagggagcg
     1981 gcgctgattg ggtatgtttt gtggcagacg atacttgtat ggggctattt tgtttatcgg
     2041 caaaggaaaa atcagacagg atggtttttt gccgccgtta ttttagcgat aattccactg
     2101 tttttaacga aagtgactcc tttttttgaa catggaaaag catcgttact tggtttttta
     2161 ggaatctctt atttaacctt taaatctgta caaatgatca tggaaataag agatggcatg
     2221 atcaaaacat ataagccatt tcgttatatc cagtttctgc tgttctttcc aacaatctct
     2281 tctggaccaa tcgatcgcta taggagattt gaaaaagatc agctccatcc gcctgttcgt
     2341 gataaatatg ttgatctttt gggaaaaggc gtccaaaata tttttgtcgg ttttttatat
     2401 aaattcatta tcggttatta ttttggtcaa gtactgcttc ctgtagtggg acgcattgcc
     2461 ttacagcatg gtggtatttc atgggcatta gtaggttata tgtacgtgta cagtatgtat
     2521 ttgttttttg attttgctgg ctatagctta tttgcagtcg gtacaagcta catgatggga
     2581 tatcagacac caatcaattt caataaacct tttttatcat ggaatatcaa agaattctgg
     2641 aatcgttggc atatgacgct gtccttttgg ttccgtgatt atgtatatat gcgattgatg
     2701 ttcttcatga tgaagaaaaa attttttaaa agcaagatcg taatgtcaaa tatcggttat
     2761 tttgctttgt ttcttctaat gggtatatgg catggattga cgtggtacta catcgtatat
     2821 ggactgtatc atgcttgtct gatctgtctg acagatgcat ggatccgatt taagaagaag
     2881 cataaagagc agttgccttc taacaaattt acgcatggct tagccgtatt tttgacattt
     2941 caagcagtat gttttagctt cctgattttt tcaggatttt tagataaact tttttttagc
     3001 taacgaatag tgacatatta atttatataa caaaggagaa aaggaaaatg gaagaacaaa
     3061 ttttatcaat cttagaagaa ataacaggaa cagatgaagt aagaaaaaat cgtgatattg
     3121 atctatttga tgaagggttg cttgattctt tagcatctgt tcaattatta gtcgagctag
     3181 acggagagct ggatatccaa gtccctgtat ctgaattcga gcgagaggat tggtgcacac
     3241 cgaataaaat cattcaacaa gcaactgcat tgaagggttg agtatgaaaa agaaactttt
     3301 tgctattttc ggcccggttg ttttagctgt tggcttactg tttgtgtttt ttacctcctc
     3361 ctatcaagta gatatctcta atccagaaac gattagacgt gcctcttctt ccatgtcagt
     3421 gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta tctgaaaaac aatatattcc
     3481 tttttttggc tcaagtgaat taagcagaat cagtccattt catccgtctg ttctggcaga
     3541 aaaataccag cgaaactatc gtccattttt gctgggagca cccgggacac aatcgttaag
     3601 ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa aacaagaaag tcgtctttat
     3661 tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact gattacttta atacgtatta
     3721 ttctgaactt caaacatacg actggttgtt ttcaatgaag aaagtaacgc ctgctgatcg
     3781 ctatttagct agaagattat taactttctc aaaagtaaaa gagaatgata cgttgacggc
     3841 gattctgcaa acgatcaaaa aagggaagct acctttacct gaatctttga atcagctgcg
     3901 cagccaatgg aacatgctga agagagagga tgaagtattc agcaatattg ggttgaaaga
     3961 tcgacagcaa aaaatcgatc atgagtcaaa acgattacca aaacaatacc aagaaacaga
     4021 attatccatt ttagcaaatc aaattggaga acgagaaacg acgaataatc cattcgggtt
     4081 gaaaaacgat ttttatacgc atcgtatccg agcccacgag ccagagctca aacagtcaca
     4141 aaaaaattgg gattatcgtt tttcgccaga attttctgat tttcagctgg tcttggatca
     4201 attagcaaaa aaccataacg aagtattgtt catcatccca ccggttaacg agaaatggag
     4261 cgattatacc ggactatctc aagaaatgtt acaaggattt gccaaaaaga tcaaatttca
     4321 attaaatagc caaggattca atcgaatagc tgattttgtc aatcaagcgg gtaccaacta
     4381 ttttatggaa gacacgatcc atctaggctg gaaaggctgg ttggctgcag atcaacagat
     4441 cagaccgttt ttagaagaaa atcatattac tgctagtaag tatcatcttg acgatgcttt
     4501 ttttagcaaa tcttggcaac atcaaatacc agataaacta caattaaaat aaagaaaaca
     4561 ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt ctgaataaaa gataaaaata
     4621 ccaagtttgc ctgaacaaaa atcataagca atggtataat cacaatatga atatagaaat
     4681 gggggaaaac atttggatac aaaagaagaa cagctactta ttgatttaac ttctgcaaaa
     4741 ggagtacctg gaaatgaaga agaggtaaga gaagtattca gagaatatgc caaaccattc
     4801 gctgatgata ttttttatga cggtttaggc agtgtgattg ccaaacatgg tgctggcgga
     4861 ggccccaagg tctttatttc tggacatatg gacgaagttg gttttatggt aacaaaaata
     4921 accgaaaaag gattcttgga attccaaact cttggaggtt ggtgggggca agtcatgctt
     4981 gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg tccacggtgt gattggttcc
     5041 aaaccaccac atgttttaac acctcaagta agaaataaac cttatgaaat aaaggatatg
     5101 ttcattgata ttggcgcatc aagccaagaa gaagccaaag agtggggcat acgtcctgga
     5161 gatatggtca ctccatatat tgaatacaaa cgaatgaatg gcagtaaata tttactggca
     5221 aaagcatggg ataatcgtat cgggacagct gtttctctgc gagtattaga gaatctatct
     5281 aaagaagcac acccaaatgt tttattcgca ggaagcgatg tgcaagaaga agtaggcttg
     5341 cgaggtgctc gaacaagtac acaccttgta aatcccgata ttgcttttgc tttagatact
     5401 ggtacagcag gagacactcc aggaatgaca ccaaaagaag cagattcgat tttaggaaaa
     5461 ggcccacaga ttttgatttt tgatgcatct atgatcccgc ataaaaaatt attgaatttt
     5521 gttattgatg tagcagagga aatggatatt ccattccaat atacagtgat ttctggcgga
     5581 ggaactgatg caggacaaat gcatttgaca agagatggtg ttccgtctgt agccattacc
     5641 gttcctgttc gttatctgca ttcccataca tcagtgattc atgaagatga ttatttgaat
     5701 acagtgaaac tagtcacaga attagtgaaa cggcttgata gtgaaacagt tgaaaaaatt
     5761 cggagttatg aataatgaat aatagaatac ctatttctga atatcggaaa aaagcacgag
     5821 tttctttgga aaatcaatgg ggaatcaata cttggcttgt tttcttatct ataatattta
     5881 cgagcgtcat ccagaacttt tttgacggtt tcagtccttt tccggaagaa agcatgcagc
     5941 agcacttatt gagttttcta gtcgataatt tacttttatt cgcattcaac tatgcattgt
     6001 tttacattgc gctagtagtt gtacggggtg gcagaccaaa aagtaacctt ttattttcag
     6061 ttttccaaag aaagtatttt ggttcgatac ttggcatcaa tttgatcaat tcagtcatca
     6121 actggctact caacagttta atctttctgc ccatgttttt aatcagcgga gtgagtactt
     6181 atatgcagct gcttttcagt tcaaatgcgg tacctacggg tgtcatgcca gaagattttc
     6241 ttgatatcag ctttgtttta tcgatgatag ggatgatttt tgtttcagca ctcatcatga
     6301 gtattgttgg cggtttattc caatttgctg catggacaaa gttcgactat cctgatttgt
     6361 cggttattca atgtttgaaa tatggctggt ttttattgaa agatagaatt ggtacgtata
     6421 ttttgttgca gctgtctttt atcggctggt atttgctagg agcaatcgct ttgttcatcg
     6481 gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc agaattttat gaacaagcac
     6541 ggattgaaaa aggaggtcca gacgcctatt ttacttagca tgtaagcagt ttattcatca
     6601 aaaaaataac ttttgtggta ggataaatct ttgaggggga atgtacgatg aaattaactg
     6661 ttttaggatg cttaggagcg tatccttata aaaatcaagg gacaacaggt tatttgcttc
     6721 aatctgacgg gttcaatttg ttgatcgatg caggtagtgc tacgctgatc aaattacaag
     6781 aacaccttga tccacttgat ctagatgctg tgatccttag ccattatcat catgatcata
     6841 ttgctgacct tggtgtatta cagtattact ggcaacttca tcctaagaga aatccggaga
     6901 cattgctttc aatttatgga cacacaaaag attccattca ctttgaagaa ttgacgttgg
     6961 aaaatgtctc aaaaggacat ccttattttg aagctgaaga attaaaaatt ggtccttttc
     7021 tgattacttt tatggaaaca atccatccag tagtatgtta cgccatgcgt atcgtagaag
     7081 aaacaacggg gaaagtattc gtatttacag gtgattcagg ttatttggaa agtttcgctg
     7141 attttgcgaa agatgctgat ttgtttctag cagataccta tctttttgaa ggaaatgaac
     7201 atcatcatgc ccatttcact tcaaaagaag ctggagaact tgcgaaatca gctaacgtaa
     7261 agaaacttgt acttacacac ctacctcagt ttggtgattt agaacaattg aaaaatgaag
     7321 cgcaaaaggc agcaggcaaa gggattgata tagaacttgc tgcagttgac aaagtgttta
     7381 acatttagga ggaagaacat gatttttgta ccaaatgaaa accacgaccc aagaatcaat
     7441 ttagcaattg aacaatattt gctgcaagaa atgcctttag atgaacctat tttgctcttt
     7501 tatatcaatg aaccttcaat catcatcggc agaaaccaaa atacgcttga agaaatcaat
     7561 cgagattatg tggaagaaaa tggcattcat gttgtccgtc gcttaagtgg aggcggagct
     7621 gtctaccatg attttggaaa cctgaacttt agttttatca tgcctgatga tggggattca
     7681 ttccgtgact ttgcgaaagt aacacagcca atcatccaag cacttcatga attgggtgtt
     7741 gctggggcgg aattgaaagg ccgaaatgat ctagtcattg atggcatgaa attttctggc
     7801 aacgcgatgt atgctaccaa cggtcggatg tttgctcatg gaacaattat gttcgactct
     7861 gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag acaaaatcga atcaaaagga
     7921 atcaaatcga tccgttctcg tgtaacgaat atcaaaccat ttcttccaga agaaaagcaa
     7981 aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga aaattttcgg cgtagagtca
     8041 gtagaagaag taaaaacgta cgaattgact gatgaagatt ggaaaaaaat caatcgaatc
     8101 tctgaggaat attatcgaaa ttgggattgg aattacggga agtctccaga tttcaatttc
     8161 tctcgccaaa aacgtttttc tatcggttcg attgaagtcc atttgaatgt ctcagaagga
     8221 atgatcaaag aagcaaaaat ctttggcgat ttcttcggac taggagatat ctctgacgtg
     8281 gaaaaaaaat tggttggaca gaaatatgat aaagcatctc tatcaaaaat agtcagccaa
     8341 attgatgtga agaaatattt tggtgcgata gaaccagaag aattgcttac cttactttat
     8401 tgaaaataaa aaatagcgga atccacatat atggattccg ctatttttta tacaggattc
     8461 gggtaatttc gagagagttg aacagtgatc cgatcaatga aagtatcttt ttcctgcgtt
     8521 agtgttcctg tgtaacgatc cgctacgtct ttcatcatca ttaagtcaaa acgttcctga
     8581 tcatctaatt cttctgtgat gcctcgacta aataggtttg tatcgtaatg tgcactatgt
     8641 tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg acaaataaat ttttcgatac
     8701 agtggatttt cgatggcatg agtttctcta aaggaatgct ctaaagcgtg gatcaaaagt
     8761 tgagccagat cgtcatcgtt gataggaagt tcttcgggaa gatctgcttg gaaggttatc
     8821 tgaatattcc gtttggcagc taacttctgg taacgcgcta agataagatt tgtcacctgg
     8881 tggttgcaaa aagaacacag catttcgaaa ttttttgctt cttctaatat cttttccaca
     8941 tagtttttag cttttgtttg gtccgtatgc attaaatctt gcagattctc catctcttgg
     9001 acaaattcca ggcgaagtgt atttacttct tggatatgct gtcgaagctg ctcataatgt
     9061 tgctccaaca aatcattttt gatttttaag aaatctactt gtctccgatc tttgtcggta
     9121 tttaagatgt gatcgattag attgtagctg ataataacga ttagaatgat aatatataaa
     9181 atcgtagacc aattgatcgt tgtttgatag atacctaatg ccgtttggat gtaaatcaag
     9241 caatggataa tggctaccaa gacgatccaa ggtgtacaag ctacataaaa acggttattc
     9301 ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag tgcttataac actagcaata
     9361 ttagcaatcg gcattaattc aggaagttct gcaggagtaa aagttgcata aagcagaacg
     9421 ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt accatttttt atagtaaaac
     9481 attcggctcc aatagtagca gatgatgaaa gctgacgtta agataataaa taaatgagat
     9541 aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta aaatatcttc tgatacagct
     9601 tcaaatccta atgccaaagc aaagcaactt aagagaataa gtgagtattc aagacgtttt
     9661 tgtttataca tcaagtagat cgtatagaaa aaggtcccta tagcgataaa aacagcgatt
     9721 atcatcgtca atacttgcgg cactgattgt gaataaataa aactaatcaa tggtccagtt
     9781 gttcctagat aaactttcgg tggcagtcca gcatagttct gataaggaga tgagactgta
     9841 agggtcaatt tttttccgat ataatttgct ggtaaatcta tcacagccaa tgagagtccg
     9901 ggattgcttt cttcctgata atctttccgt tggtataaaa tcttttcgcc tagatataca
     9961 gtaatccatt gatggtttcc tttgattagt aaatttgctt cgtccaacgg tttttcaagt
    10021 gtcgtttcca taatgaatgt ttcgtcttta tccacggtag gaatcgtacg agtgtacttg
    10081 ctgtcaaact taaaatcagg atcatctttt gtatagaaaa cccacgaatc ttttatttct
    10141 ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa agaaataagc aattcctgtt
    10201 aaaaataaca at
//
LOCUS       DACCXM010000094        10189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCXM010000094 DACCXM010000000
VERSION     DACCXM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.096"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            220..1068
                     /locus_tag="IVY61_002108"
     CDS             220..1068
                     /locus_tag="IVY61_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP7971189.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1213..2154
                     /locus_tag="IVY61_002109"
     CDS             1213..2154
                     /locus_tag="IVY61_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP7971190.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2144..4315
                     /locus_tag="IVY61_002110"
                     /pseudo
     CDS             2144..4315
                     /locus_tag="IVY61_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(4427..6598)
                     /gene="copB"
                     /locus_tag="IVY61_002111"
     CDS             complement(4427..6598)
                     /gene="copB"
                     /locus_tag="IVY61_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP7971191.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(6618..8804)
                     /locus_tag="IVY61_002112"
     CDS             complement(6618..8804)
                     /locus_tag="IVY61_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP7971192.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(8804..9013)
                     /gene="copZ"
                     /locus_tag="IVY61_002113"
     CDS             complement(8804..9013)
                     /gene="copZ"
                     /locus_tag="IVY61_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP7971193.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(9026..9466)
                     /locus_tag="IVY61_002114"
     CDS             complement(9026..9466)
                     /locus_tag="IVY61_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP7971194.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9540..10079)
                     /locus_tag="IVY61_002115"
     CDS             complement(9540..10079)
                     /locus_tag="IVY61_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7971195.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         2872 a         2163 c         1830 g         3324 t
ORIGIN      
        1 tcgtattctc atttcaaaac gaatgtactt taaaaaaaca taataagatc actttgcttg
       61 taggatgtat tcccttccat tacaatgaaa taaatagcga ttttatgaga aggtgatgtg
      121 aaaatggaag aaaaaataga agaatccaaa caaaaaatcg atcagttgat ataagaatta
      181 gaaaaagcgg tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca
      241 ggtggtcatg tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca
      301 ctagcaaaag ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct
      361 agtgatattc ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg
      421 ggaccgatct ttaccacgat cttgttagca gatgagatca atcgtacgac tcctcgtact
      481 cagtcagcac ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac
      541 cctctagaca atcatttttt tgtcttagct acgcaaaatc caatcgagta cgaaggaaca
      601 tatcctttgc cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct
      661 tcttttgatg acgaactttt gctacttttg gataaattcg aaaaagtgtt ggataactta
      721 aatgaagtat tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt
      781 gtcagtcccg aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagtcatgct
      841 gccatccagc ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct
      901 tatgctttaa tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct
      961 tacgtgtttt cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaagaaa
     1021 caaatcattc atactcttgt tgcacaaatt cctattcctg tgaggtagat tgaggtagac
     1081 aatggaaaaa atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat
     1141 tgtgcttttt gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac
     1201 tctttatgta tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac
     1261 ttgtacattg cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc
     1321 aaaaaagaga actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt
     1381 tgcagaaaaa aacaagagat tttgattctt acaaataaac cgcaagaagt ccaattgctt
     1441 tggcagccaa aagaaagagg atgccatgaa acattaactg tcatttttac agcctatgat
     1501 agtctaggac ttttcagaaa aagtgcaaaa agagagatct cttttcctgt gactgttctg
     1561 cctgcttttt gtaagattca agcagaaaaa ttacagagag ttctggaaaa aaaacagcag
     1621 ctgaacgcgt accaaagagg tcttgatttt cgggaacatc gcgcttatcg tccaggtgat
     1681 tctttcaacc gaatcgattg gaaattgtct gctcatactc aagagtggtt ataccgtgaa
     1741 tacgattatc aagaagaaga atgttctcct cttttttgct tttggggttc gcccagtcca
     1801 aaatttgaac ctctgctaga cttgtatttt tctttatggc atttgagaaa agaaaagcag
     1861 ccggaacttc tgatattagg caaccgcgcc tttcatggca aagaccccgg tcatacctat
     1921 tttgcttctt tggaagctgg cgatgagaaa gcttttttcg cacacttgaa aaaatatgca
     1981 aaaaaacaaa tcgtcctctt tgttcctgag aattctcctg aagtctcaga agctattgaa
     2041 acattgtcaa aagatcgaac ggtttatctt gtttatttcg tagaaaaaca actcatggtc
     2101 caagaaaaag acaagatatg ttcattgcca ggaggtgaat ggattgacga ttaaacggga
     2161 aaaatggctg cgtggcgttt actcctttgt gatactaagt gtaggtgctc cgatatttgt
     2221 agtagctaat caattgcaaa acagctacct tcttatccta actattggtg tcctatgcct
     2281 tattgtctgc tgtattccta aaaaatgggt acgtttgctg ctttctttgc cagtaatgat
     2341 aggttgtttg tttttatatt ttccttcgaa gacattttcc atattatgga tcttacagtt
     2401 tttttcgtca gtcacagagg aattcttcca tctatcccaa ggtaaacttt attatttgcc
     2461 agaaaagaca gctttttttc tcataatgtt gctgatctat ttatttatta cattgaccgt
     2521 ggattacgac tattggtacg caccctttct ttctttgatg gcttacttca tgatgcttac
     2581 cgtttttcgg aaaaaagatt tactgtacga aatgattgcg gtagtaactg ttttgttcgt
     2641 gtatctggca gcaaggcaat ttttctccat ccggttgttg tcgggaaaca ctcgattcca
     2701 gtcattgact acagttctgc ttatttgttt cttaactttt agcgctatct tgcctttata
     2761 tcttccgaaa gtacaccaat ctctcgagga aacgagtgaa ccgatccgag aatatcttaa
     2821 tgatgaaggg ctttatgaca cactccatga gtatcaatta accggaagtg caagaacagg
     2881 cttcagtgag aatgatgaac agcttggtgg acctttatat gataatggcg aagttgtttt
     2941 tacagcgaac caaaaaggaa atggctattg gaagatcgaa aataaaaatc attacaccgg
     3001 ttctggatgg atagaagatc aggacgcacc catccaacca atccaagaat tccctgttga
     3061 catcacgagc taccaaaccg ttatagaaga caaagaaaac attacattga ccctaaaaaa
     3121 acaagttttc ttaccttatc cttatggaaa tactaccttg actgattttt cattagctag
     3181 tggacaaacg ctttactatt tgcctgaaag ccagcgattc attcctgtcc cgaatgaatc
     3241 gacacgttct gcaactttac aaacagaaag gttagttttt acgagagaag aattaaaaag
     3301 cagtcagtta aaagctgatg atttagaaaa tcagcagaca aatcttcaat tgccaaaaag
     3361 tttgcctaaa cgtattgagg acttggcggt cgaattgacc aaaaatgaag caactttaat
     3421 tcgtaaagtc gaagctgtct aacagtattt aaaaacaagc ggaaatttcc gctattccaa
     3481 aacggatacc gggcatactc caaaagaaca cgattatgtg gattatttcc tctttgattc
     3541 aaaagtcggc tattgtgaca acttttcaac ttctatggtc gtccttcttc gaacacttgg
     3601 tatcccagct cgctggacaa aaggttttac aaatgggctc agaacaggca cagataatca
     3661 agggaatgag gaatacagca ttctcaatag tcatgctcat tcttggccag aggtatactt
     3721 tcctggattt ggctggcttc catttgaacc aaccccttca tttgccaatc caatgggttc
     3781 acaaccagtt gctgaaacaa ccgaaaccgt ccagcctgct atcgaacaac caaatgaaag
     3841 cgaaaatatg gcagaaacta tttccagtga gagatctgag gtcattccat cagaagaaac
     3901 aacactcaca ccagcttata aaatcgtctt attcagcctt ggagtgtgtt tatccgtctt
     3961 gttgttcttt ctattgttat tcagaaaatc tctgcgctgg acaagttatc ttatctatct
     4021 tcattttttt gcttctacta atacgattgc atatcaaaaa cttcttaacc ctttggaaac
     4081 attggtaccg cgcaaacccg cagaatcttt gcaggaatat agcctgcgaa tagaacaaga
     4141 attcaagcta gatctgcgtt tcattcgatt aactactcaa tatgaagcag aaatatatgg
     4201 acgacaagca gatccggtaa aattcgataa aaaaacctat ttagaaataa cgcatataat
     4261 aagaaaaatg gtatacaaaa aaattctgac aaaagggagc cgcttcaaaa aataagataa
     4321 aatttctgga gaatctactt ctggaatgcc gagaaccagc tgattgagta tcgattactc
     4381 gaaaaaaagg ttgtgacacg ttttttgtca caaccttttt tcattattat ctgattttca
     4441 atgtcaaagc atttagagct acaactaccg tacttaacga catcaagaca gcaccaactg
     4501 ctggacttaa tatgatacca attggcgcaa gaattccggc tgctagcgga attgcaagaa
     4561 tattgtaacc tgccccccac cagaggttct ggatcatttt ctttcgcgtt tgcttggcta
     4621 gatcaagaaa atgcaaaata tcttttggat cgctgtttgt caatacgaca tccgcagaat
     4681 cgattgcgat atctgtccct gcaccgattg ccatcccgat tgaagcacgg gctaaacttg
     4741 gcgcatcatt gatcccatca cccaccatga ttactttttt cccttggtca aggtatttct
     4801 ggacaacagc ttctttatca tctggtaata aaccgccata atattcttcg atccctaaat
     4861 aatctgctac tgcttgggca gcgtttttat tgtcaccagt aagcatgaca ggagtgattt
     4921 ttctttcttt caatgttcgg ataaattctt ttgcttctgt ttttactacg tcacctaaag
     4981 caacgattgc agctagatga ttttctaaaa tcaggaagct gatggtattt ccttgttctt
     5041 ggtaaggttt caataatgct tgttctacgt ttaatccgag acgctcaact tctttttcat
     5101 taactatttt cacttgttgt tttttgactg ttgcctctag cccgactcct gaaagatttt
     5161 ttagattttc agcttgataa ggcttgattt catgttcttt caaataattc ataatgccaa
     5221 cagccaatgg atgattggca ttcgcttcca aggctcctag atattgaagg atttcatttt
     5281 tattgaacgc atcatcaagt acttcgacac ccgttacagt aaattttcct tctgttagtg
     5341 ttcctgtttt gtccagcatg atcacatcta gatcattggc ttgctccagt gcattgcggt
     5401 ttttcaaaag taatccgttt ttagctgcta ttgatgtgga acgagcaaca actaatggga
     5461 tagctaaacc tagagcatga ggacaggcga tgataaagac tgttaccata cgttctaatg
     5521 cattaggaag atctgtcaag aacaaccagg cgatgaatgc caggataccc acaattaatg
     5581 cgacataaaa cagccatttt gccactttgt cagataatga ctctagcttg gatttttctc
     5641 cttgtgcttt tcgcaccatt tccataacct tcgctagata accattttca ccagttccag
     5701 ttacttcgat ttcgatcgtt ccatccccat tgatagaacc gccaatcact ttgtcattgg
     5761 cttgtttttt gattcctctt gattcgccag tcacagctga ttcgtcaaca attgtttcac
     5821 ctttcaagat tacaccatct gttggcattt tatcaccaga ccggacgatc aaacgatcac
     5881 cttcgtttac ttctttcaat gaaactgtct cttcttttcc atctttcgtc aagcgtttca
     5941 ctgattctgg cagtaattct gctagttttt gtaaagcatt gctggcattc gacacagcat
     6001 tcatttcaat ccagtgacct agtagcataa taacgatcaa agtagccaat tcccagaaga
     6061 aatccatgac gtgtgtatgt ggattgagca gatttgcgat aaacgaatag acactataaa
     6121 tatatgcaac tgtaatcccc atcgcgatca gtgtcatcat agcaggactt ttttgtttca
     6181 attccatctt agctccactt aaaaatggct gaccgccata gataaatagt atagttgcta
     6241 agatcaatac gagccaatca gaaccaggaa acgttacttg aaatggaaat tccattccca
     6301 tcattggtga aaaaagaatg attgggatag ctaggataag cgatagccaa aatttttgtt
     6361 tgaaattccc catgtgcatg gaatgatcca tgccgccgtg ttcgtgatcc atgtgcatcg
     6421 aatggtccat ttgtttgctg tttgctatat ctccatggtt atgtcccatg tgcatggaat
     6481 gatccatctg actatggtca tgtttcatct ccatctgttt ttcatgagat tctgtacttt
     6541 ctttgtttac tacttctttt ttatgctcat gttttttctc catatcgttc ccattcattt
     6601 ttactccatc ccttctctta ctttttcatg tgtcgattta agcacaggga gttcaacaat
     6661 acactgacgg aactaaacgc catcgctcct cctgcaatga ttggattcaa aaagcctagt
     6721 gcggcaaaag gaataccaat cgtattatag acaaaagccc agaaaagatt ttgtttgatt
     6781 tttctcaatg tcaacctaga caactcgatt gtttgtgcaa tggatgccaa actgctattc
     6841 atcagcgtca catcggctgt ttccatcgca atgtccgttc cacttcccat tgccataccg
     6901 acatcagcta atgctaaggc cggtgcatca ttgattccat cgcctgccat accgactttt
     6961 ttgccatctt tctgaagttt ttctacgtag gctgctttat cctctggaag tacttcagca
     7021 aagatatgct ttggatcaat accaacttgt ttaccaatcg tttcggctgc cagtttgttg
     7081 tctcctgtca gcataaaaac atctaatcct ttattctgga gctgcttgat tgcttgtttg
     7141 acttctagtt tgatttgatc agccacggca atcaacccga tcacttgctt ttcatcagct
     7201 aaaaacatga ctgtttttcc ctgctgttct aattctaacg cgtgttcttg gtattcatca
     7261 aatgataggt tcaattcaat taatcgttta cgtgtacccg caaaatagcg tactccgtta
     7321 atcgtaccgc tgattccagc acctgggtgg gctgtaaaat caatcatatc gtatgttgct
     7381 gcttcttgca gttttccgta ttccacgatt gctttaccta gtggatgttc agaagcttgt
     7441 tctaaagtat aaaaaataga taggacttct ttagaaccga ctagatctgt aactttgggc
     7501 tttccttctg taatcgttcc agttttatcc aaaacaatgc tgtctaagtg agcagctgct
     7561 tctaatgctt ctccgccttt aattaaaatt ccatttcgtg caccaacacc cgttcctacc
     7621 ataatagctg ttggagtcgc taaacctaat gcacatggac acgcaatgac taatacagag
     7681 acactatgga tcaatgcgag ttgccaatca ccagtgatca aacctgtagc tatgagcgtg
     7741 ataaacgcga tgactaatac gatcggaaca aaaatccctg agatccgatc tgcgattttt
     7801 tggattgggg ctttgctccc ttgagcatct tccaccattt gaatgatttg cgctaatact
     7861 gtctgcttgc cgacttgtgt aacttccatg tgaagtagac cattcgtatt gattgtcccg
     7921 ccaaagagcg tgtcatccgg attcttttct acaggaagat tttcaccagt aagcatactt
     7981 tcatctatcg cactgcttcc tgaaataatt cgaccatctg ctggaatctg ctcaccagga
     8041 cggatgacta gttgatcacc aacgactact tcttcaatcg ctaacgtttc ttctttcccg
     8101 tttcttatca cttgggcggt ttttgtttga agagacatca attgtttgat tgcattacct
     8161 gttttcgttt ttgctgtatg ttccaaatat ttgccgagca aaatcagcgt gatgatcata
     8221 ctgctgcttt caaaataaag atcttggggg tgtccacgga aaaatccgtt gtaaatgctc
     8281 aatgcaaagg cagccgatgt tccaattgct accaatacat ccatatttgg tgctttcgtc
     8341 ttgattgcat ggtacgcacc tttataaaaa cgagcaccga cataaaattg gacaggagca
     8401 gacagcacta actggacgat tggaaaatga aaaaagtgaa caatcgccgc atggcttcct
     8461 aacatcatag caatcattga caacatcaac ggcaaagtca aaaccgtact caatataaga
     8521 tcgaatagca tttttttcaa atacgcttgc ttttcttctg caattttttg tttgtgggct
     8581 tcatcgtaca aaattgcacc atagccgatg ttttctacgc tttggattaa tttttcagtg
     8641 gtcgtccctt catatttgac acttgctttt tcagtggcca agttgactgt ggcattgacc
     8701 acaccaggtt gtccgttcag ctctttttcg atcctagcag aacaatttgc acaagtcata
     8761 cctgtaatga cgaacgtatc cattttggtt tctgctgttg ccatcaaatc acctctgctt
     8821 tataacccaa tccattgatt gcttgacaaa tcttttcgga ttgaactttt gtttcatcaa
     8881 acttgaccgt accgtttgct tttttcaaat ttactttcac tttttgcacg ccgtcaagtg
     8941 cgcttactgt ttcttctact cttgctgcac aatgattgca gctcatacct tcgattgaaa
     9001 attgttgttt cattttttct tcctcctatt gatgttgttt acattcacat tgacctggga
     9061 tacagttaca ttcgatcgta tctactggtt gcttttgttc cagttgtttc aaaatctcat
     9121 tgatatcttc ttgagtcaaa gtggcttctt ctactagatc cgcaatcgtc tctccaatcc
     9181 tttttgcaca gatatgggaa aaaagatttt ccgtcgcact gcgaactgtt tccatctccg
     9241 agacagctgg ataataaatg aatttttttc cttcttgttc tgtccagatc acttcttttt
     9301 ttaccaaacg acccagcaaa gttttgatcg ttgctggttt ccaatccatc gaatcagcta
     9361 agatctgcgt gatttcttgg gctgtcgtct gtccaagtgt ccaaataaca cgcatgatct
     9421 cccactctga atcactgatc tttactggcg ttttgacttt ttccatttat ttcacacctt
     9481 tcgtttacaa atgtaatcat tatcttatga atacagatta cacttgtaaa caaaaactgt
     9541 caattatttt tgctctctat tttcaattta gttaaaaatt cttgtttctc tcgtcttgtt
     9601 ttaaggatga tcagtttatt tcttgcatta gctacttgga tcagctgctg tattttcggt
     9661 tcattgtttt cttcaaaatg ccagataaaa gataaaaatt ctaagtattc cctgtttagt
     9721 ttctccgaaa atgattcagg catatctggc cgagattttt tgtttatctt tctttttaga
     9781 gaacgccaaa ctactcggta gatggcgaga taacgcggca ctttaagcca aatgatctta
     9841 tctgcttctt taatcctttc ctctaatgta gaagtataat ttccttcaac gatccatgac
     9901 ggattagata ccataaacaa ttgctgttct tgcaaaaacc acttttttgc ctgaatactg
     9961 tagtctgttg tatgccataa aaggtctagc ggcagtagcg gaaagtcggt gatttctttg
    10021 atcttccttg cgagggtcga ttttcccgac ccactttgtc caatcacgat gatcttcatt
    10081 cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac gccgcaaaaa
    10141 aacaagggac tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaataa
//
LOCUS       DACCXM010000095        10153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCXM010000095 DACCXM010000000
VERSION     DACCXM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.097"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            343..567
                     /gene="nrdH"
                     /locus_tag="IVY61_002116"
     CDS             343..567
                     /gene="nrdH"
                     /locus_tag="IVY61_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP7971196.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            726..2885
                     /gene="nrdE"
                     /locus_tag="IVY61_002117"
     CDS             726..2885
                     /gene="nrdE"
                     /locus_tag="IVY61_002117"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP7971197.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2935..3900
                     /gene="nrdF"
                     /locus_tag="IVY61_002118"
     CDS             2935..3900
                     /gene="nrdF"
                     /locus_tag="IVY61_002118"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP7971198.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3989..5128)
                     /locus_tag="IVY61_002119"
     CDS             complement(3989..5128)
                     /locus_tag="IVY61_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7971199.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5437..6150)
                     /locus_tag="IVY61_002120"
     CDS             complement(5437..6150)
                     /locus_tag="IVY61_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP7971200.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6404..7105)
                     /gene="nagB"
                     /locus_tag="IVY61_002121"
     CDS             complement(6404..7105)
                     /gene="nagB"
                     /locus_tag="IVY61_002121"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP7971201.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7339..8871
                     /locus_tag="IVY61_002122"
     CDS             7339..8871
                     /locus_tag="IVY61_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7971202.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(9062..9241)
                     /locus_tag="IVY61_002123"
                     /pseudo
     CDS             complement(9062..9241)
                     /locus_tag="IVY61_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9261..10153)
                     /locus_tag="IVY61_002124"
                     /pseudo
     CDS             complement(9261..10153)
                     /locus_tag="IVY61_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT         3354 a         1950 c         1774 g         3075 t
ORIGIN      
        1 ttttcttacg tgttacgcaa ataaatgaga atcattctca catagaatac tctcggtaat
       61 tgagaatgtc aattatactt gtgatattat gagcgaatct ttagtattga tagataaaaa
      121 tgagaaaaaa atatttcttc atagttttcc acaatttttt ctctattaaa tttgctattt
      181 tcccacacga tacttgtttt cagctctttt ttcaaaaaca caaaacttag tattccacag
      241 tttccacaac acaatatgta gtgtttgttg tttgactttt ttactaagag ttttatacta
      301 tctattatag ataataagaa tgtgttagag gaggcactca ccatgaacgt aaaaatcttt
      361 tctaaaaata attgtatgca atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt
      421 gcttttgaag aagtcaatat cgatgaacag cctgaagcag tagactggtt gaaagcacaa
      481 ggattccaaa gtgttcctgt cattacttct gatgcagaaa cagttatcgg attccgtcct
      541 gatcagttgc gtaaacttgc cagctaacat gactaaaaga atcaaaacgt gatacatgga
      601 tttgaagggc ttgagacagc accagttttt gtcgcaaact tccaaatcct tttttaaagc
      661 ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa tacgaaatga aggttgggcg
      721 tacgtatgag tcttaaaaat ttacaagatg tcagttattt caagctgaat aatgaaatca
      781 atcggccagt caatggtcaa atccctttga ataaggataa agaagcttta gttgcctttt
      841 ttgaggaaaa tgtcaaacca aatacgatga cttttccatc tattatggaa aaaatcaatt
      901 acttgatcga tcaagaatat atagaagcag aattcattca attgtattca ccatcattta
      961 ttgaaaagct atatcaattt ttggacgagc aacatttttc tttcaaatct tttatggctg
     1021 cttacaaatt ttattctcaa tatgcgttga aaaccaacga cggttccgct tatctggaaa
     1081 cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc gaacggggat gaagaattag
     1141 cacttgctct tgcagacgaa atgatccatc aacgatatca gccagctaca ccttctttct
     1201 tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg tttcttgact caagtcactg
     1261 acgatatgaa ctctatcggg cgctcaatca actctgcttt gcaattgtcc cgtattggtg
     1321 gcggcgtagg gattacttta tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg
     1381 agggtgctgc aagtggtgtc gttccagtca tgaaattatt cgaagatagt ttcagctatt
     1441 ctaatcaatt aggtcagcgt caaggagctg gcgtggttta tttgaacgtt ttccatccag
     1501 acattattgc tttcctttca gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa
     1561 ctctttctct tggcgtgatc gtacctgata aattttatga attgacacgt aaaaacgaag
     1621 atatgtattt atttagtcca tatagtgtcg aaaaagaata tggtgtccca ttctcttatg
     1681 taaatattac agctgagtat gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa
     1741 tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca acaagaatct ggctatcctt
     1801 acgtaatcaa tattgataca gcgaaccgcg agaatccaat cgatggcaag atcatcatga
     1861 gtaatttatg ttcagagatt ttacaagttc aaacgccttc tgtgattaac ggcaaacagg
     1921 aatatgaagt attaggaaca gatatctcat gtaacttagg ctcgacaaat atcgtgaatt
     1981 tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat gactcgtgca ttgacattta
     2041 ttacagatgc atctgatatc gatgtcgtac caccgatcca aaacggaaat aagctgaatc
     2101 atacgattgg attaggtgcc atgggcttac acacttatct tgcaaaagaa caaatggaat
     2161 atggctcaga ggattcatta gactttacaa atatttactt catgctgttg aattattgga
     2221 cacttgttga aagcaacaat atcgctcgtg aaagaaaaca agttttccat aattttgaaa
     2281 aatcagctta tgctgacggc acttatttcg aaaaatacgt aactggagaa ttccagccaa
     2341 aatcagaaaa agtcaaagca ttgtttgaag gcatttttat cccaactgct gaagattgga
     2401 atgctttgaa acaagcagta atgaaagatg gactgtatca tcaaaatcgc ttggcagtag
     2461 caccaaatgg ctcaatttca tatatcaacg atacgagtgc aagtattcat cctatcactc
     2521 gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat ttattaccca gcgccttatc
     2581 tatccaatga gacgattccg tattacactt ctgcttatga tatggacatg cgtaaagtaa
     2641 tcgatgttta tgcaacggca caacaacacg tcgatcaagg catgagtttg actttattca
     2701 tgcgttctga aatcccagaa ggcatttatg aatggaagac aacaacgaag caaacgacac
     2761 gtgatttgaa tattttaaga cattacgctt tccacaaagg aatcaaatcc atttattacg
     2821 tacgtacctt tactgatgat gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga
     2881 tctaatatca tagatagatt cgcttattca tattttaatt aaaggagcat tctcatgtca
     2941 gaaacatatt atgaagcaat aaactggaat gaaattgaag atatcattga taaatccact
     3001 tgggagaagc tgacagagca attttggcta gatacacgta tcccactttc aaatgaccta
     3061 gatgattggc gtacattgtc tcaattggaa aaagatacag taggacatgt atttggcggt
     3121 ttgacactgc tagatactgt ccaatctgaa agtggcatgg atcagttgcg taaagatgtc
     3181 cgcactcccc atgaagaagc cgttttgaac aatattcaat tcatggaatc cgttcatgcc
     3241 aaaagttatt cttctatttt tagtacactg aatacgaaaa aagaaatcga agaaattttt
     3301 gactggacaa atacgaatcc ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac
     3361 aaacacggta caccgctaga aaagaaaatt gccagcgtct ttttagaaac attcttgttc
     3421 tattcaggtt tttatactcc tctttattat ttaggaaaca acaaattggc taacgtagca
     3481 gagatcatca aactaatcat ccgagatgaa tctgtacatg gaacctacat cggttacaaa
     3541 ttccaattag gattcaacga gttacctgaa gcagaacaag aaacgttaaa agactggatg
     3601 tacaatcttc tttatgagct ttacgaaaac gaagaacgtt atacagaaga attatacgat
     3661 ccaatcggct ggacagaaga agtcaagaca ttcctacgct ataatgccaa caaagcattg
     3721 atgaacctag ggatggatcc actctttcca gatactgcca atgatgtcaa cccaatcgta
     3781 atgaacggga tttcaacagg cacaagcaac catgacttct tctcacaagt cggaaacggc
     3841 tatttgttag gtcatgtaga agcgatgaaa gacgacgatt atttgattgg attggaataa
     3901 ggatttattt taattcaaaa atttttcgaa aaataaaaag agtttagcgt acagatattt
     3961 taaatgtaca ctgaactctt ttttatgctt attcacttgt ttcttccagc acttttggac
     4021 tgtctaactt ttccgaaaca tgttccattc ttctcaataa atagattttc cggatattta
     4081 atattatcgt cttaatcact gcatagacag gtatgcaaat cagcatgcct aatattcctg
     4141 cgacacttcc tgctccaatc aataccaaga tgatcgtcaa aggatggata ttcattgatt
     4201 tcccaaataa caacggcttg atcaaatttc catctaattg ttggatcact aaaatcgaca
     4261 acgccatata caaagcttga aatggagaga taaacaaacc gacaatgatt gctggtaccg
     4321 ctccaataaa agggccgaca tacgggataa tattcgtgat tccgcaaatg accgcaagca
     4381 acaagctata aggctgatcg aatattctca ttgccaaaaa gctcattaca ccaataatca
     4441 aagcatctaa gatcgtactg ctgatatagg cagataatgt ttcgtttaat tcttttaccg
     4501 tttggcgtaa ctctttacga atcgctttcg gaaagaagtg agacaacgca tcaataaatt
     4561 tgtgcccatc cttgaacata aatagcaaaa taaatggaac ggtaaataac aaaataaaaa
     4621 attgcatcat aaatgaaatg atttttgaga cacttgcaga taaactgact aatatgatat
     4681 tgaacaaatt actaagagat aaatttgctg atgctaattg ttcttccaaa ttgaaatttt
     4741 taaactcttc ccgctgcgcc aattcattta accatttgct tgtttcatct gcatacattg
     4801 gcaagctctg ggtcaattga attgtctgtt ccattaactg aggcactaca ttcatgacag
     4861 ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa aaaaccgaac actctcggga
     4921 tccgatgctt ttcgagaaaa acaacgattg gatcgaacat ataatagata aaaccagcaa
     4981 caagcaatgg taaaaaaatt gccgaaagca ttttcaatat tggactaaaa atattcggca
     5041 tttgtaaaat aaagtacacg ccgatgatcg tgagtactag ctccactgtc cagaaaaata
     5101 attttgattg tttaaaacgt gattgcatac atctcctcca aggtcctttg atacttctat
     5161 tctatcattt aacaaagagt tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa
     5221 aataaacata ttttaatgag tagacgagta cttaaacaaa aacacggttt ggagtagtga
     5281 caaaaattat actgcagtcc ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg
     5341 taataagccc agccaaacga aaaatataag gaactatttt tatttggctg ggcttattac
     5401 ttgatgtagg acatctattt tacaacctca gatcatttat tgcgcaatac tgactaaatg
     5461 caactttgtg atatttttgt gtacggacaa ctcctcgatc acatcggcat aagtgagccc
     5521 ttttggaaga tcaatcgtat agatattttt gtacactcgg tagtcatcaa cgaattttac
     5581 gtcaaaattc acatcttcga ttacgatatt tttctcttca aaataatcgg taagaaaatc
     5641 tttggtttct tttctatgga taaattggat ttctaacttt ttagtcgtag gtacatggat
     5701 cacgcgtttc actactgtga gtgcaatagc aatcccgata aaactgatga tcgcaatgga
     5761 atagtaaccc atgccgatgg cgatccctaa tccagcaatt gcccataaag aagcggcagt
     5821 cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac
     5881 accactcacc acttgagcaa tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg
     5941 tggatactct aaagcatctc ttaatgcacc actagcgatc tcaacttgga tcaatgcaat
     6001 gatacatgct cccatacaaa ctaaaatatg cgtacgcata cctgctggac gatttttata
     6061 ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg atccctaaac gtaaaataat
     6121 ctctggaatt gtcaatacca tatcattcat ctaaacaccc tccagtccaa tatatcatgt
     6181 ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt acgaaaaaag gactgtgaca
     6241 aaatttttgt catagtcctt tttatgaata aacggtgttc aatcagctga ccctcggtat
     6301 tactcaacaa acggcaaaat atggggagct atttttcctt tgtcttctct aatactcagg
     6361 tcaaaacgct gatttcacaa cctctttagc ctactattga aaattacaac ttacttgctg
     6421 cttcttcatc gatgatcaca acaacattgt cgtgattttg caatacactt gctggcaaat
     6481 gattcgttac aggtccttca atcatccctt tgattgcatc agctttgctt tcgccaaatg
     6541 ccatcaagac gatttcttta cttttcataa ttgaaccgat tcccattgaa acagcttttg
     6601 ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc attgattgtt gattctgtta
     6661 agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc
     6721 cattttgccc gattcctaag atttggatat cgattggatg tgcatcgatg atttgatcat
     6781 actttttgca tgctgcttcc agatcttctg cttttccatc tggtacaaac gtttctttga
     6841 atggtttttt atcaaataat tggacatcca tgaaatgacg gtagctttgt tcatctgaac
     6901 cgcctagtcc aacgtactca tctaagttta cagaagtcat ttcactgaaa tccaaatcgc
     6961 ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc tgttgccaat cctaatactt
     7021 ttgcaccttt gttcatacct tctttgatga tttcaaatgc tttttttccg ccttcttcag
     7081 cgttttttac acgaatgatt tccatagtga ttgctccttt atattttggt atagcccaat
     7141 tatagcattc tttttttctt tttacaataa ttagtataga ccaaattgca tttttttcat
     7201 tttcttgaca ataaatagga atccgtgtta ggtttcataa gatggttcaa tcaaacaatc
     7261 catgttgcat gaaaaccaag caagtgattc actgtggacg ctttatgtct gcagtttttt
     7321 tatgaaaaag gagttattat gagtatactg acattaaaca acattaccca gcaatttggc
     7381 gacaaaatcc tctatgaagg tgtcgatctg caattaaatg ctggcgaaca tcttgggctg
     7441 attggacaaa acggagccgg taaaagtaca ttgattaaaa tcatcactgg tgagatctta
     7501 ccagatgacg gacagatcca ttggcaaaaa aatattcaca aaggttacct cgatcaatat
     7561 gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa
     7621 tacgaaaagg aagaaaaaat tgcttttctt taccaaacat atagcgagac cttcgatgat
     7681 tccttgctgg aaaaagctgg gaaactccaa acggaattag accaaggagt gttttatcaa
     7741 atcgacacct tagttgctga gatgagcagc gggttgggga tcgatgtctt aggtttagat
     7801 tcactgttga aaaatctaag tggtggacaa cgttcaaagg tgattttagc aaaactactt
     7861 ttagaaaacc cagatgttct gatcttagat gaaccaacca atcacttaga tgatcagcat
     7921 atccaatggc tgacgcagtt tcttcaggat tttgacggtg cgtatatcgt gatttcccat
     7981 gaccaagaat ttcttgatca gatcaccacc catatcgcag acatcgagtt tggaaagatt
     8041 actaagtata ctggccatct aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc
     8101 tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga
     8161 aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc gacaaaaaca attagataaa
     8221 atcgaacggc tgacgcctcc agcttcagca agtaaagctg tctttgattt tccattctcg
     8281 cctattgtca cgacattagc agtggaaaca actgatttag tcatcggata tgatcgaccg
     8341 ctacttgcac ccatcaattt gaccatccgc tttggtgaaa atattgccat tcgaggcttc
     8401 aacgggattg gaaaatctac tttattaaaa acactgatcg gtgagattga aaaaataagt
     8461 ggcgatttcc acttcccaga taacacaaaa atcaattact tttcacaaga tctgtactgg
     8521 gaaaatccat tagaaacacc actacaatat ttaagcagtc aatttccaaa agcgactatc
     8581 aaagaattac gtcgccaact agctaaagca gggttagtta atcaattagc aagtgaacct
     8641 ctcactacat taagtggtgg ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac
     8701 caaggtaatt tactgattct cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa
     8761 agcttacaag acgggataca gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa
     8821 gaattttatc aagatcttgt gcaacgagtc attgagattg aaccaaaata aacgtttgat
     8881 tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc acaactcctt cttctgaata
     8941 aatggtggcc aattagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc
     9001 tatttttatt tggctattct tattacttga tgcagaacag cttttcacaa cctcttttcg
     9061 tctatttttt attttcgaga tttctctttc ctttaaaaac aactgtttga aagaagaacg
     9121 cacatccttg aaaaacaagc ccgctaaccg ttacacctaa ccagccatac tgctgccaca
     9181 ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa gtacataaac ataaatacag
     9241 acccgtggtg ctagttaatt ttaatatcct aaagaatatc tcaaagttcc ttcaaaacgt
     9301 cgttgtgctc gacttaatag aacactgtaa gccagtaata tgctttcaaa tctactctct
     9361 agccctttaa cagaacgtga gttgaaattt tgacaaccta atttttctaa ggaagaaaaa
     9421 acagtctcaa ctgtttttct ttgttttcca agtaagctgt ggtctagttt ttctgattta
     9481 tccatgtttt tacgtggcgg aacggatata gtaatgccct taagggcaag cttttcatgg
     9541 attttatggc taatatatcc tttgtcccct aggatattgg gaagattcgc ttcttccgat
     9601 aaagtctcta acactttcac gtcatggaca cctggattcg tgattgagta agtaatcgga
     9661 aagccagtct tagtaacgat catatgaatt ttaagaccat aaaaataaga tttttttgtt
     9721 gagttatagc caactttggc tatttgattc aatagttttg cttgtctatt tctaactggt
     9781 ttacataatg gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc
     9841 ttgatgaaaa agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg
     9901 aatcgactcc tactaggaaa gtcaccatta ggaaataaga cggaacaaac cgctctatac
     9961 gtggctcttt ggctagtata gccattaatt atgccccata taacacaagc aataatcact
    10021 gtatcgcgtt acttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa
    10081 ttgtggtata tgttagaaac agttctcata atttccttga atgttataaa agtatctgta
    10141 taatgttcag tat
//
LOCUS       DACCXM010000096         9977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCXM010000096 DACCXM010000000
VERSION     DACCXM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.099"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            160..3387
                     /gene="fss3"
                     /locus_tag="IVY61_002125"
     CDS             160..3387
                     /gene="fss3"
                     /locus_tag="IVY61_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP7971203.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3500..3814
                     /locus_tag="IVY61_002126"
     CDS             3500..3814
                     /locus_tag="IVY61_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7971204.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3827..4201
                     /locus_tag="IVY61_002127"
     CDS             3827..4201
                     /locus_tag="IVY61_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7971205.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4211..4546
                     /locus_tag="IVY61_002128"
     CDS             4211..4546
                     /locus_tag="IVY61_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP7971206.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4629..5969
                     /locus_tag="IVY61_002129"
     CDS             4629..5969
                     /locus_tag="IVY61_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7971207.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6047..6721
                     /locus_tag="IVY61_002130"
     CDS             6047..6721
                     /locus_tag="IVY61_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7971208.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6954..7388
                     /gene="dcm"
                     /locus_tag="IVY61_002131"
     CDS             6954..7388
                     /gene="dcm"
                     /locus_tag="IVY61_002131"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7971209.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            7389..8096
                     /locus_tag="IVY61_002132"
     CDS             7389..8096
                     /locus_tag="IVY61_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7971210.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            8086..8376
                     /locus_tag="IVY61_002133"
     CDS             8086..8376
                     /locus_tag="IVY61_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971211.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8635..9819
                     /locus_tag="IVY61_002134"
     CDS             8635..9819
                     /locus_tag="IVY61_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7971212.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9834..9953
                     /locus_tag="IVY61_002135"
     CDS             9834..9953
                     /locus_tag="IVY61_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7971213.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         3303 a         1681 c         2252 g         2741 t
ORIGIN      
        1 taaaggcagt atatttcgta ttttagatta aatttttagg ctcattcatt ctcaaatcca
       61 ttctattcag tcaataaaaa ttttaaaaag actttataaa caaagatgtt caagcgagca
      121 tcttattttt ttgtgcttaa tttgaaagga gaacatttca tgtataaaaa acaaaaaaag
      181 aaattatgtg tattaactat tttgagtttg gctgttcaag caattgttgg agcaattgct
      241 cctacgatta cttttgctga tgaaattacc catccacaaa cggtaaccgt agaactagat
      301 ttagcacatc aatatgctgt tgaaggaact tttagtgatg gtcgtccaat gtcagaagtc
      361 actgttccgc attatgcagt gtacaacggc tttaaacaag atattttctg tattgaacca
      421 ggagtcccga tttacaatga gttcactcct ggctatgaga aaaacccgtt accagatatg
      481 tccgataaag ccaaattagt ttccgtttta tggaaaaaag taggtacaga tgcagatact
      541 catattgtgg cacagaagat gatctggcaa gaagttaatg ggtataccct tcactcaata
      601 aaacgatcag atggtagtgc cgtaaatatc gcagccattg aggcaaaaat aaatcaagcc
      661 attgacgatt atcagaagaa accaaccttc cataatagta cggctaaaac agtattaggg
      721 caatcgacta ctgtgacgga tacgaataat ctgaatttgt cagagttcga tgaggttgtg
      781 gaaaatgccg caaacattga ttatcgtgtg aatggtaatc agttgataat caccccaaat
      841 gccaattcaa aagaaagtgg tgtgctgacg cttaagaaat ctgctggtac cggaacacct
      901 gtcgcctata aaaaggctgg ccaacagact ttaatggccg gggcgattga taaacctaat
      961 acctacacaa tcaaaattga tgtggaaacg gagggttctt taaagatcaa aaaagtcgat
     1021 aaagaatcgg gtgatgttgt accaggaacg gttttccatt tagactttgg aaaaacttta
     1081 cctgcaaaag acgtgactac tgacaaagaa ggcattgcta cattggatga gattcctcat
     1141 ggtacaaaag taaccattac cgaaaaatca gtaccggcgc cttatacgat tgataccacc
     1201 cccatgacta ccaccattaa agcaggcgaa accatttacg tgacttcaaa aaacgcacgg
     1261 gaaaaagggc aaatcattct ggataaaagc ggagttgaaa cagggagtga tctttggaat
     1321 gacaattatt ccttagctgg taatacgttt gccattcgca aagacagtcc aaccggtgaa
     1381 atcgttcaag aaatgaccac ggatgaaaac ggtcatgcgg aaacaccaaa agagattgct
     1441 aatgcgttgg aattgggaac ctattatgtg accgaaacta aagctagtca tgggttcgtg
     1501 aatacgttca aacctgtaaa agtcgaattg aagtatgcca atcaaaccgt agcccctgtt
     1561 accagtaacg taaaagggca aaatcaagaa gtcactggcg aaaccacttt gacgaaagaa
     1621 gacaaagata ctggggataa agcacaaggt aaggcagtct ttgagggtgc tgaatacacc
     1681 ttgtttactg ctaaggatgg aaaagctgtt aaatggagtg aggcttttaa accagagtta
     1741 gcgaagggaa cgaaaggttc tgatgaaacg gtcactttga ctttagacga aaagaaccaa
     1801 gttgccgtta aacatttagc tatcaacgag tattattggc aagaaacaaa agcgcccgaa
     1861 ggatatagcc ttgatgaaac aaagtatcct gtatccatca aaaaggtgga tgatagcgaa
     1921 aaaaatgccg tgattacccg aaatgttacg gcaaaagaac aagtgattcg ctttggcttt
     1981 gatttcttta aatttgctgg atcagctgcg ggcactaccg aaactggctt caatgatctg
     2041 acctttaaag tgtcgccatt ggaagggacc aatgaaatca caggtgccga agatgaagcg
     2101 accacagctt ataacgagca attagggttt gatggttatg gcaagtttga aaatcttcct
     2161 tatggggatt atttacttga agaagtagaa gccccagaag gttttcaaaa gattacgcca
     2221 ttagaaatcc gttctacttt taaggaaaac aaagaagact ttgtgaagag tgagtatgtc
     2281 tttaccatta ccgaacaaga ccaaaaacaa ccaattaaaa cggtgaccgt tccttacgag
     2341 aagctgacga acaaagcatt ttctgttagt ttgaaccgtt taatgctcta tgatttgcct
     2401 gaggaagaag atagtttaac ttctcttgcg acttggaaag acggaaacaa agaattaact
     2461 tctctggatt ctaccgagct agttgataaa ttgagttaca atctacatga aatcaaagaa
     2521 gactggtatg tggtagcgca agccattgat gtggatgcga caaaagctgc ccaagaaaaa
     2581 gatgaaaaag ccaaaccagt ggtgattgca gaaacaagcg caacgttggc gaacaaagag
     2641 aaaacaggaa cttggaaaat tcagcataaa ttaaccgccg aacaagtttt aaataaaact
     2701 attgtcttat tcaactatgt gtatgaaaat aaggaagctt ttgaagcagg cgataagcca
     2761 gtagcgaagg atgttagctt gaataaccaa gcccaaactg tcagttgtac agtggaacac
     2821 catgtttcta tccaaacaaa ggcccaccta gaaaacggtt ctcagacctt tactcatggt
     2881 gatgtggtgg atatgtttga tgatgtgtcg attactcatg atgtactgga tggctcaaaa
     2941 gaagctttcg aaacaattct gtatgcttta ttaccggatg gtacaaacaa agaaatttgg
     3001 aaatctggca agattgatta tgaagtaaat gataaagaat tcaccaaaac tgtgcttgcg
     3061 aaaaaagtag ataccggaaa atatccagaa ggaaccaagt ttacttttgc ggaaatcaat
     3121 tacgataaag atgggaatat caatggaaaa cacaatgaag atttgaaaga aaaatctcaa
     3181 accttaacac caaaagaagt tccaaccata ctaagtacgc cgaaacaacc ggaaacacca
     3241 actgttccaa gtggctctca agaatctagt cccacagtga agaccttccc tcaaaccggg
     3301 gagaaaaatt ccaatgtttt actgttcatt ggttttactt tgatttttgc gacggcgggc
     3361 tattatttct ggaatcgccg gaactaaggt gatgagatga tacaagtaaa tcctaagaac
     3421 caacgtgcgc gaccgcctcc taaaaaagta gtcgcgctat ttttgtatcc aaaactaaca
     3481 atgaaaagag gaaaagaaga tggaattgaa atttgtcgtg ccagatatgg ctgaaacctt
     3541 tggaaagtta agttatgccg gtgaaggaga aattataaca gaaggatatg gacggaatac
     3601 tacggtgatt ggtcgcagtt atcatttgta ttccagtaag caacgggccg atgatattga
     3661 ggtggtagta gctgcggaag ccggtgaaaa ggattttgat caagaccaac cgctaaaagc
     3721 cgtgaatccc catttggttg ccaaaggcta tgagattgag aatcgtgggt ttactgatta
     3781 cgtgttgtat gttgatgatt tagtgaaagc ataggaggaa aagaagatga gattaacaga
     3841 aggtattgtc gtagattcag gtttaacatt tgggaagttg cgtttttccg cattacgccg
     3901 agaagtacgg aagcaaaatg aggatggaac aattggtaat gaggtaaaag aacgaaccta
     3961 taatttaaaa tcctctgcac aagggcgaat gattcaagtt agtatcccag ctaatgtacc
     4021 cttacgcgag tttgcttatg atgcagaggt agagttggtc aatccaatcg tggataccgt
     4081 tgccaattat gtttttcgag aaggaactac cgttaattgg ttcattaagg cggatgattt
     4141 agtcttgaag cggcagccga atcaaggaaa tcctacgaac caaaatgaaa gaaagaaata
     4201 ggtgaagatt atggaaatta atgttgaaca tattttagat tgtctcgatc aatatggaaa
     4261 aggcgagctg actgaggaac aactcgttaa agcattaacc tctgatgaaa aaatgtttct
     4321 gattatacat caaggtttac ttggagaaaa tagcgacagt gaagaagact ctgatgtact
     4381 agactggtta ggagaagaag agtctttctt tatagttgtc gaagtaaatg aaaacttatg
     4441 tcggcaggca gaatccattt tggaagaaat tggtgtggag atgccggatg ctattgaagg
     4501 ctttttaaat caattggttg aaacgaaaca actcccctcc tcgtagcagt aaatgactag
     4561 cataactttt tgaaattgcc taatgaaatc ctattgggct ttttcgattg gaggtggaaa
     4621 ccatcaaaat gtacaaagga catcgcatac gcgcaggaga tcagcacttg gtttatcact
     4681 ttgttcttgg ctggctactc gctctgttca tcggttggat gagcgtcttt tattttcaag
     4741 agtttagaca atttgatatt tctaagctat cactttctac tattgaaatc gtttggtcta
     4801 taaaagattt agtatgtctg cttggaagtc tcgctttttc aggagctatg atattgcttt
     4861 atattcactt ttttcttgat cattggcgaa gcttgtggca tcgacaaaag ctagctcgga
     4921 tgattctaga gaaccattgg tatgaagtga aacaaactca aagtgaaggc tttttcaaag
     4981 atttgaatag tagccgaacc agagagacta tcagttactt ccccaaaatc tgttatcgaa
     5041 tgaaagatgg attactgtct attcgcgttc aaatttcact tgggaaatac caagatcagc
     5101 ttttaaaatt ggaaaagaag ttagaaagcg gactgtattg tgagttagta gaaaaggaac
     5161 tcaaagactc ctatgtggag tatactttac tgtatgatat gatcgccaat cgaattggga
     5221 tagacgaagt agtggcagaa aatgggactc tgcgattgat gaaaaatcaa gtatgggctt
     5281 atgattcttt accccatatg ttaatcgctg gtggcacggg tggagggaag acctatttcc
     5341 tcctcaccat tatcgaagca ctattgaagt cagatgcaga attgtttatc ctcgatccca
     5401 aaaatgcaga tttagctgat ttaggtacgg tgatgcctca tgtttattca caaaaggaag
     5461 aaatttctgc ttgtgtggaa gatttctatg aacgtatgat ggctcgtagc aaggcaatga
     5521 aagaaatgtc taactacaaa acaggagaga attatgccta tcttggactt ccacccaact
     5581 ttttaatctt tgatgaatac gtggcttata tggagatgtt aacgacgaaa gaaagtgcgg
     5641 tgattttgaa taagctcaaa caaatcgtga tgttgggtcg tcaatctggt ttctttctga
     5701 ttttggcttg ccaaaggccg gatgcgaaat atttaggaga cggaatccgc gatcaattca
     5761 actttcgcgt ggctttgggt cgtatgagtg aactcggata ttccatgatg tttggggagg
     5821 tcgaaaaaaa cttctttatg aagcacatca aaggtcgagg ttatgtggat acaggtggca
     5881 gtgtgattag tgagttttat acgccacttg taccaaaagg gtatgatttc ttagattcaa
     5941 ttagtaaatt agaaaagatg aatgattgag tgatcttcaa tactgacttt atacttcaag
     6001 taacgtataa tacttttata tagtaggaag aaaggggata attaatatgg aaagtaatga
     6061 ttcttattta gagtatgcaa aagcaaataa ggataagttt atttcttata ttatcaaagg
     6121 gaaatccccc gaagttggac gagacgcagt ttttatggca gggagtcctg gggctggaaa
     6181 atcagaggtt gccattggat tagctgagaa ttatgataac catgtgatta ttgatgcgga
     6241 ttactttcgc acccagtttc ctgattataa tggaaaaaac tctagtctct ttcaaaaagc
     6301 atcttcttgg ttggtagagc aggcatttag atatgtctta gatcatggct attcttttat
     6361 tctggatgca acttttgcaa tattaagtgc tgaaaaaaat atctcgcggg cgttgaaaaa
     6421 acattttcga gtgacgattt tctatgttta tcaagaccct aaaatcgctt ggaaatttac
     6481 acaagaacgt gaaattgcag agggacgtca tgtgccgaaa aaggcattta ttaacgcttt
     6541 tttcaaatca agagagaaca ttgcaaaagt aaagagtaaa catccagatg tattgcttca
     6601 tatcataata aaagattata cgaataatat ttccgaagtt cattatgcta cggataatct
     6661 agaattattt ttaccactag ggtatagtag gaaagatttg gaggttcaat tacatgactg
     6721 agaaagaaca agtaacgaaa attgtaaaga aatacaataa gagcattgcg gatttatcgg
     6781 aaaatgctac tgcaaaagag ttcaaaacgg tgattaaata tgtagccgat caggccaatg
     6841 aaaaacaacg gaaattagtt ggattaaata aaaaatagta ggacaaatta ataatgtatg
     6901 agccaagagc aaaatgttct tggctttttt ctttgcaatg aatgaggtaa tgtatgagat
     6961 ttttagattt attcgccggt attggtggct ttcgtttagg tatggaacag gcgagccatc
     7021 attgtatcgg tttttgtgaa attgataaat ttgctagacg tagttataaa gcaattcatg
     7081 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagatgag tttattcaat
     7141 ccctcggacc agtggacatc ctttgcggcg gatttccgtg ccaagctttt tcaattgcgg
     7201 gaaagcggca aagattttcc gatactcgag gtactttatt ctttgaaatc gctcggttca
     7261 ccactcttct ccgacctaag cttttattcc ttgagaacgt ccgaggattg ctcaatcacg
     7321 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgacgtgg
     7381 agtggtaggt gcttaactca aaggcctaca ttccccaaag ccgggagcgg atattcctta
     7441 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga ccgtcttcaa
     7501 catctgatca aaacatcaga aatttggtaa atatcaatcc ttccaatcga ggaatgggtg
     7561 gacaagtcta tggttcagat ggcgtagcgc caaccttaac aggtgatgaa ggaatcaaaa
     7621 tcgcactgcc agcaagggat ggaatctctg tagcaggact attaccggga aagtttgaac
     7681 aaggtaatcg agtttatgaa gttactggaa ccgcccccac cttgtcaacg aaacaaggtg
     7741 gtacaaaaat catgattcga aaaaatgaaa cagaatatca agaggtgaag ccagaagaca
     7801 gtgtgaatct ggcttttcct aattccacaa gtcgccgagg tcgtctggga aaacagagtg
     7861 ttcatacctt actaacagga gaccaacaag cgatcgtcac ggatcagtat caaattcgga
     7921 agctaacacc tagagagtgt tggcgacttc agggctttcc cgattgggct tttgatcgag
     7981 attctcaagt caattcagat agtcagttgt ataagcaagc cggaaactct gtaacagttc
     8041 cggtcatttt tgatattgcc agaagattaa aagagttgga tgtacatggt ttatgatgag
     8101 ttgattggag aaatttattg ggtgattgga aagattcagt cagacccaga attagaagaa
     8161 caattgcgac ggttaaaatt tgatattcgc aagaatggtg tcaaagtcgc tggcgatcca
     8221 tatttaatga atgaggagac ggatgctcga attgaaataa atcaagtaat tgcagaattt
     8281 gagcggattg cggaccaagc aaaagaacca gatattcgtc aatacttgtt agaaatgaaa
     8341 gcagagttgg aagtaaatgg tattacagat gagtaaccta aaccaacaca actgactgtt
     8401 aggcgctgac cgcggaggat tcgcgaagca gaaatcctcc gtggtgctgt gcctgttcat
     8461 ggacggcgtt agccgaccat gatgtctcac ccccgtatct aacagggggg tacaaaaaca
     8521 caaaaaataa gtaagcaacc attaagaatg cttgttgtat caaagatagt aagtaatttg
     8581 tataggtgtt aactcatttg aaaaagttaa caccttgaat ttggaggtga gaacttggca
     8641 caacggaatt tagattatcg gctattaaaa gaccgacgca acgaatacgg cgtttcacaa
     8701 aataaattgg ctacggcttg tggacttagt cggccgtact taaatcagat agaaaatggt
     8761 ggcgtaaccg catctataaa aaccatgagg aaaatttttg accaactgga aagctttaat
     8821 cccgatttgc ctttgacgtt actgtttgac tatgtaagga ttcgctttcc cacaacggat
     8881 gcacgaaaaa ttattcaaga aattctccat ttgaaatttg agtatatgct ccatgaagat
     8941 tatgcctttt actcctatca agaacagtat gtcatgggtg atattgtggt gatgttgtct
     9001 catgaagaag ataaaggtgt gcttttagaa ttaaaggggc gaggttgccg acaatttgaa
     9061 acttttttac tcgctcaaaa gcgtagctgg tacgactttt tcgaggattg tctaaaggcc
     9121 ggtggcgtga tgaaacgctt ggacttggca atcaatgatc gagtggggct attggatata
     9181 cctgagctaa cgaaaaaatg tcagaaggaa gaatgtatct ccttgtttcg tacctttaaa
     9241 agttatcgtt ccggggaact tttaaaggct gatgaaaagg acggcatggg caatacctta
     9301 tatatcggca gtctcaagag tgaggtttat ttctgtttgt atgaaaaaga ctatgaacaa
     9361 tatatcaagt tagggattcc attagaccaa actgagacga aaaatcgttt tgagattcga
     9421 ctgaaaaatg accgtgcttt tcatgcgatt caagatttgt taaaaggtcg tagcattgag
     9481 agcaccacgt tttccattat caatcgttac ttgcgatttg cggataaagt agaaggtaaa
     9541 aggcgaacca actggccctt gaatgaacaa tgggggcgtt ttattggacg gaatcggaaa
     9601 gagattcaat taacttccga gccaaaaccg tataccattg aacgaacctt gaattggcta
     9661 ggccgacaag tcgcccctac gtggaaaatg gctaaggagt tggatcgatt gaatcggaca
     9721 acctatatcc aggatatggt acagaatgca cgcttatcag accgacataa aaagattttg
     9781 gagcaacaaa gtatggcaat tgaaaatttg attatatgaa aagaggaatc gtcatgggaa
     9841 ttgtaaaaga tatttttttg gtatttggtg gcagtatgct tggtgttact gtgatgtgct
     9901 tgatgaatgc aagtgcagca gcggatcgag caatggaaaa gaaaggaaag tgacaagatg
     9961 aatttcggac aaaattt
//
LOCUS       DACCXM010000097         9888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCXM010000097 DACCXM010000000
VERSION     DACCXM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.100"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(109..2229)
                     /locus_tag="IVY61_002136"
     CDS             complement(109..2229)
                     /locus_tag="IVY61_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7971214.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="IVY61_002137"
     CDS             2459..3637
                     /locus_tag="IVY61_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP7971215.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="IVY61_002138"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="IVY61_002138"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP7971216.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="IVY61_002139"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="IVY61_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP7971217.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="IVY61_002140"
     CDS             complement(4990..6243)
                     /locus_tag="IVY61_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7971218.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="IVY61_002141"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="IVY61_002141"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP7971219.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFISGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="IVY61_002142"
     CDS             7821..9167
                     /locus_tag="IVY61_002142"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP7971220.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="IVY61_002143"
     CDS             complement(9475..9861)
                     /locus_tag="IVY61_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7971221.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3150 a         1800 c         2024 g         2914 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt atttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttatta
//
LOCUS       DACCXM010000098         9802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCXM010000098 DACCXM010000000
VERSION     DACCXM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.101"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(190..1038)
                     /locus_tag="IVY61_002144"
     CDS             complement(190..1038)
                     /locus_tag="IVY61_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7971222.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1202..2104
                     /gene="gnd"
                     /locus_tag="IVY61_002145"
     CDS             1202..2104
                     /gene="gnd"
                     /locus_tag="IVY61_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP7971223.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2106..3107
                     /locus_tag="IVY61_002146"
     CDS             2106..3107
                     /locus_tag="IVY61_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7971224.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3104..3388
                     /locus_tag="IVY61_002147"
     CDS             3104..3388
                     /locus_tag="IVY61_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7971225.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3421..4218
                     /locus_tag="IVY61_002148"
     CDS             3421..4218
                     /locus_tag="IVY61_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7971226.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4232..5065
                     /locus_tag="IVY61_002149"
     CDS             4232..5065
                     /locus_tag="IVY61_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7971227.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5083..5940
                     /locus_tag="IVY61_002150"
     CDS             5083..5940
                     /locus_tag="IVY61_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7971228.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5962..6471
                     /locus_tag="IVY61_002151"
     CDS             5962..6471
                     /locus_tag="IVY61_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7971229.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6508..6909
                     /locus_tag="IVY61_002152"
     CDS             6508..6909
                     /locus_tag="IVY61_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971230.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7021..7374
                     /locus_tag="IVY61_002153"
     CDS             7021..7374
                     /locus_tag="IVY61_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971231.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7408..7851
                     /locus_tag="IVY61_002154"
     CDS             7408..7851
                     /locus_tag="IVY61_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7971232.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7868..8953
                     /gene="uxuA"
                     /locus_tag="IVY61_002155"
     CDS             7868..8953
                     /gene="uxuA"
                     /locus_tag="IVY61_002155"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7971233.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8946..9659
                     /locus_tag="IVY61_002156"
     CDS             8946..9659
                     /locus_tag="IVY61_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7971234.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3256 a         1554 c         2166 g         2826 t
ORIGIN      
        1 ttgttatcgc atctaacaag taactcgaat aataccaata caaccttctc caaagatgcg
       61 atgacatacg ttctaaccca ccgactattg atgttctgta ccacccttgc agaaccaaaa
      121 aagccactac caaatatttg gtagtggctt tttttgtttt atggattttt cgctttttct
      181 gttatggtgc tattctttga tttcagaaat agtatgtcga actttagcga tagaacgacg
      241 tgattctttt tcgttgtaaa acatcaaaat cacatataga gagtccaaaa tgcttagttg
      301 agaaatacgg gaagctaaag cttcagatcg gaattctgtt tcttcagaaa tagaaataaa
      361 aacgacatcg ccaagtttag caagggaaga attagcttgg ctagtaataa cgattgtttt
      421 tgctccagtt tttcttacgg tttcagcaat atggattgtt tcttttgatt gccctgtgtg
      481 agaaatcaaa acagcacagt ctttttccgt caataaagaa gcttccatca attgaatatg
      541 gtaatccgtg ctgtgagaag ttgcgatagg ggaacgcaaa aatttatgat aagcgtcagt
      601 tgctacaatc tctgaaccgc caacaccgaa aaaatagaca cgtttagatt cattgataat
      661 agtagccgct ttttttagat ccttttgttg taacaacttt tttgtgtcat ttaaagttga
      721 aatactagac tcgaacacct tttgcgccat agtgagctca gtatctgttt tctgaatatt
      781 ttcgtggata gaaatagcag atagatcatt ttcttggatc aacatggcca ttttaaaatc
      841 tttaaatcca ttataaccaa gtttttttgt aaattgaaaa aaagtagagt cagcaatgcc
      901 cagttcactt gctaaattgc taataggact atgacttact ttgcttggat tttctaaaat
      961 ataatcggcg atagcctgtt cttttgggct gaagtcgttg tacattgttt tgatttgtag
     1021 tcttatggag acgctcatga gaattcctca ttttcataat aagattatca ttttttccgt
     1081 tctcattaat gttaaagata caaggagagt aggaaaaaat caaacaaaaa atatttttgt
     1141 aaaatctatt tacaaaaaaa tttttttgta taatgagggt ataagaaatg gaggcgctat
     1201 catggaaatt ggtgtaattg gtttgggaaa aatgggattg aatttagctt taaacttaaa
     1261 agagcaccat cacaaagtac aaggactcga catttcaagt acagctgtta gtcaggcaag
     1321 agaaaatgac attcctgctt atcaagagat ctctgaattt ctagctactt ttgagcagaa
     1381 aaaaatcatc tggttaatgc ttcctgctgg agagataacc gaaaacatgt taagaaaact
     1441 ttttccaaaa ctcggaccag gagacattat tattgaaggc ggaaactctt attataaaga
     1501 ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc ggttacttgg attgtgggac
     1561 ttctggagga atcgaaggag cacgacatca agcctgctta atgattggag gagataaaga
     1621 aacttttcta tccgtggaac aattattcaa agatgtagca attgaaagag gatatctgta
     1681 tgctggtgca tccggaagcg gacacttctt gaaaatgatc cataatggaa tcgaatatgg
     1741 aatgatgcaa gcaatcggtg aaggattcca acttgtacaa aaaagtgagt atgcttttga
     1801 cttggaacaa gtcgcacgtg tttggaatca tggttcggtg attcgcggct ggctgatgga
     1861 aatcatggaa gatcagttaa acgaacatcc caatttaagt aattatcggg gaatcgtttc
     1921 tgcatcaggt gaagcaaaat ggacggttga aacagcgcta gaaatggctg ttccagttcc
     1981 agtaattgca actagtttgt tcatgaggaa tctttcacag gaagaagata gtttttcagc
     2041 aaaagtagtt tcagcactaa gaaatgggtt tggcggacat gaaatagttg aaaagggtga
     2101 gtaaaatgcc agattatcat atagcaatcg ttaattccag cagtttcgga agagtctttc
     2161 cacaccactt ggaaagatta aagtcagttg gagacgttca tttctttact gttgatcaag
     2221 agattccagg aaaagaacta gcggagttac ttaaaggatt caatatcatt attgcaagtg
     2281 ttacgccatt ttttcgacga gatttttttg aaaacaaaga tgaactgcta ttgatttccc
     2341 gacatggtat tggttacaac aatattgatt taaaagcagc aaaagaacac cagacaatcg
     2401 tatctatcat acctgcttta attgaacgaa atgctgtagc agaaaataat ataactaatt
     2461 tgttgtccat gcttcgacgc acgacacaag cacaagcaag agtcaaggac gatcgatggg
     2521 aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt gtgattggtg
     2581 caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc tgtgatgttc
     2641 tagcttatga tccttacaaa tcagatttat acatcgaaag ctttggtgca aaaaaagtag
     2701 aacttcaaga attactggaa caatcagata tcatttgttt atgtgcgaat ttgacagaag
     2761 agaactatca catgatttct gataaagaag tcgatcagat gaaagaaaat gtttatctat
     2821 ctaatagtgc aagaggcgct ctagttgatg aagaagcgat gattaatggt ctgaaaagcg
     2881 gaaagatcgc aggatatgcc acagatgttt tagaagaaga accgggacgt caagatcatc
     2941 cgtatttagc gtttgaacaa gtagtgatta cgccacatat atcggcctac acaagggaat
     3001 gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga acagatcagt cagcaccttc
     3061 ttcctgaacg ttctgtccag acaaagagca actatataaa aggatgatga agaaaatggt
     3121 gaacggttta aaagtgaaaa caggccctca attttattta tatgaagaag gtggtatttc
     3181 aaaagtttct gatctcttga aatcatacgg cgcgaaaaga gtactggtca cccacggaac
     3241 ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa cgattcaatt
     3301 tttttatcat cgttattctg gagaatgtag ttatgctgaa gccagacgaa tagctacaat
     3361 catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact gaccgatcta
     3421 gtgggttata gtgccaatct tgcaagaatc gatttcggag tcatccctac tttagctagt
     3481 aattgtgctc catggacacc tcttgctgtc atgtataagg agaatggaca agcagaaggg
     3541 aaaacagaac acttttacag acaagctgct ttttttataa cagatcccca attagtgatt
     3601 gattcccctg ttccatattt cattgccggg ttagcagata cactagcaaa atggtatgaa
     3661 tctgagctca tcttagaaca agattttttg caagatgaat catttttgaa gctagcacaa
     3721 gatactgcga agatctgcaa agaagagatt ttgaatcatt ctgctaaagc aatccaagat
     3781 atggcacaaa gaaaactgac atcggagttc aaacagcttt cagagattat ctttgctgtt
     3841 gctggtttag ttggaggatt aggagataaa tacgcaagaa atgcggcagc acatgctatg
     3901 catgacgcta tcagtaaata tattccggaa agccatcgtt ttctacatgg agaaaaagta
     3961 gcgtatggga ttttctatca actggcacta gaaggtcgct gggaaaaaat agacgagtta
     4021 tatcctttgt atcgggaatt agaactgccg gtttctctag aacaaatggg attatttccg
     4081 aaagataaac agataataga taaaatggct gaatttattg attcgaaaga gaaagtgcat
     4141 ttgattccta tagaggtaac acagaaatca ctgatacagt caatgatgga actggaaggt
     4201 tatatcagaa agatctgaag gaggaggaca catggaaaca ttaagtgtag tacaaagttt
     4261 gttgattgct ttatgggtag ccgcggttat gtcccgctgg ttaggtggag gagccacgtt
     4321 gaccttacgt ttttctcctt tgatgactgg attagtcgtc gggttgatca tgggggaagt
     4381 tgcacaagcg atgattgtta cagccgcttt gcaaatgatt tatatggggg ttttttctcc
     4441 aggaggatca atgccagctg aaccatctat tgcagcagct atcgcagtac cagtcgcatt
     4501 attaggggat ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg gattgctcgg
     4561 aagttacttg tatcagtttc gttttttcat caatactttt ttaggtaaat atacagacaa
     4621 agctgtagaa gatttgaata caaaagctat ggcccgatca attattcttt atccaacggt
     4681 tgcctcattt ttattattcg ttccgcttgt tttcattgct ttatatttag gggcgccatt
     4741 gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt atccacattc ttgaagtagt
     4801 aggtggaggg ttagcggcaa tcggtatcgc gacaactgta tacgttatcg gtcgaaaaga
     4861 ttatctggtc tttttcttcc ttgcttattt tatgagtgtt gttttgaaat ctttagatat
     4921 tacgatggtt acttatgcca tttttgggat tattatcgct ttgatctttg tccagtcgca
     4981 aaaagggaaa caagtacagc ctgccacaag caataacaat tcagcatctt ttgatgacga
     5041 agatgactat gatgacggat tttaatttag gaggaatggg aaatggctga aataatggat
     5101 aatcaaacag gtgctcctga agaaatcacg aaaaaagatg taacaaaagc ctatctgaga
     5161 tggcattttg caaacgaaat tcctcactct tttgaacgtt acttggcgcc atcactacta
     5221 tacggaatga tgccaatctt gaaaaaactt tataaagatg aagactcttt aaaagcagca
     5281 tataaacggc agttactgtt cttcaacact caattaagct ggggtggcgg tgtgattact
     5341 ggcctaatgt cttctatgga acagcaaaga gcagaagaag agtatgaaga aaaagagatc
     5401 ttgatgcagg atgacttgat gtataacacg aaagcgggat tgatgggggc actagcagga
     5461 atcggagatg caatcgattc aggaaccgtc caatatattt ttatcgcaat cgcggtacct
     5521 tgggcacaac agggaagtcc tttaggcgca ctttttcctt ttatctgctt tgggttgtat
     5581 caggttttac taggggtatt tttcgcaaga caagctttca ctatgggccg taatgctaca
     5641 ggcctaatgc aaaacactgg tgttcagaca gcgattgaaa tgctatcagt tttagggttg
     5701 tttatgatgg gggtgcttgc gggaaactat gtaaaagtag aatcttcatt gaaatttgcg
     5761 atatccggac gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc agggatatta
     5821 cctttagctg tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt gaaagtcaca
     5881 caagcattat tatggctgac tggaatatta attgtgctag caggaatcgg tattttataa
     5941 aaagaaggga acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga tgagcgtttg
     6001 atccatgggc aagtaatggt aacgttatct caaaaaagtg gagtgaattc catttttgtt
     6061 gtagacgaag ttgtagcaaa agataaattt atgagagatc tttacaaaaa tgcaggaagc
     6121 cgtacaggac aaaaaacaat tgtcattaca ccagagaaag ctaaattcta ttgggatgaa
     6181 tatcaattta aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac gatttacgac
     6241 ttagtaaaac acggaatccc catggaagaa ttgaatattg gagggattgc tcaaaaaaat
     6301 cctgatacag atcttttagt gacgaaatct gtttatttaa ataaatcaga tgctgaaaaa
     6361 ttgaaagaaa tgaatgaaca atatggcgta aagaatattt attttcaagc cactccatct
     6421 gcacctaaaa caggattaaa agatgtattg aacaagtttg atttgtcata aacaaatgct
     6481 tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg tgattgggaa
     6541 tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca gcgttttctt
     6601 tcagcattag tatcagatat ttacagtatg agaacagatg tgacagtcat tgcttacgat
     6661 acgctagcct accgatcaaa aaatatttat gttggagata tcgaaaaagc agagaaagtt
     6721 atctgtactt attacgatac acccgttcat gcattaggaa gttatttcat gtttgattgg
     6781 aaagaccaaa ggaaaaaaac aatctactct atattattgt ccttcatttt gttgttcagt
     6841 ttaggttggt ggggaatgat gatatataac aaaaatcctc atcacgtatt cgatctgcta
     6901 tccgtataaa caggtatcac tgtactagca tttgggagct actttttctt attgggaaaa
     6961 gcagctcgcg gatggcctag cagacaaact tttattcgaa atacttcatc catattaacg
     7021 atgctagaaa tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt tgttgatgaa
     7081 ggctgttatg gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa agaggctatt
     7141 cttttttatc tagactctgt tggggcagat acaccgctac agttttcggg aaattatttt
     7201 tcaaataaag aacagtggct gaaacaagtt gataagttga aagaaaaaaa cgtcaattat
     7261 atttttagtg caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac agatttacga
     7321 gggaaaacat ttaattggca aaatgccaat cagattattg ctctttttcg ataagtaaaa
     7381 agtgcaaaac aaggagtgag aagactaatg ttggaaatca tgatcgttac acatggtaag
     7441 ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag ccatattgaa
     7501 acaatgtgct tagagtctgg ggaggacatc caaacattcg gagagaaggt caaagaaaaa
     7561 ttaactatga tcaacacagc agaacccatg ctagtatttg ttgatctaat tagcgcaagt
     7621 ccgtacaatc aagcgctctt ggcagtcaat gaactgactg aagaaaagaa agcagccact
     7681 tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag caatcaatca tcagatgctt
     7741 cacacaccga tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga taatttaact
     7801 gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta gaaggaaggc
     7861 tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga tcccattaaa
     7921 atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga ctaaaatgcc
     7981 tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg agagccaagg
     8041 actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg cagggacaga
     8101 tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt ctcgatgtgg
     8161 ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa ctgatttgtt
     8221 taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg tcgatcagat
     8281 ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca aattgccggg
     8341 ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg aaggaatgac
     8401 cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc cagtgtgcga
     8461 aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac tttttggttt
     8521 tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag tagattctcc
     8581 atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa acgatttagt
     8641 tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa acgtggagtt
     8701 tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct cccttgatat
     8761 gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac gccctgatca
     8821 tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg atcgtgcaat
     8881 gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac cactgggaga
     8941 gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta ttagaaactg
     9001 gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat gaaatgcgta
     9061 aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa caaatgggca
     9121 ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat caatataaag
     9181 aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa gaaattttat
     9241 cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca cgggtaaaag
     9301 tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc atttcagggg
     9361 atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt tcaaaaatga
     9421 atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc gaagcagcaa
     9481 ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg cccgatcata
     9541 atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa gcagactatg
     9601 aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg tatctctaat
     9661 aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag ggaacagatg
     9721 aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac aatttagaac
     9781 aaacacaaaa cgcatctact gt
//
LOCUS       DACCXM010000099         9721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCXM010000099 DACCXM010000000
VERSION     DACCXM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.102"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            338..1387
                     /locus_tag="IVY61_002157"
     CDS             338..1387
                     /locus_tag="IVY61_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7971235.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1384..2265
                     /locus_tag="IVY61_002158"
     CDS             1384..2265
                     /locus_tag="IVY61_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7971236.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2268..3038
                     /locus_tag="IVY61_002159"
     CDS             2268..3038
                     /locus_tag="IVY61_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7971237.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3317..4171
                     /gene="aroE"
                     /locus_tag="IVY61_002160"
     CDS             3317..4171
                     /gene="aroE"
                     /locus_tag="IVY61_002160"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP7971238.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4349..5512
                     /locus_tag="IVY61_002161"
     CDS             4349..5512
                     /locus_tag="IVY61_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7971239.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5629..6039
                     /locus_tag="IVY61_002162"
     CDS             5629..6039
                     /locus_tag="IVY61_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7971240.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6213..8441
                     /gene="topB"
                     /locus_tag="IVY61_002163"
     CDS             6213..8441
                     /gene="topB"
                     /locus_tag="IVY61_002163"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7971241.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8579..8896
                     /locus_tag="IVY61_002164"
     CDS             8579..8896
                     /locus_tag="IVY61_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971242.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9056..9292
                     /locus_tag="IVY61_002165"
     CDS             9056..9292
                     /locus_tag="IVY61_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7971243.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            9289..9687
                     /locus_tag="IVY61_002166"
     CDS             9289..9687
                     /locus_tag="IVY61_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7971244.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3379 a         1650 c         2064 g         2628 t
ORIGIN      
        1 aattcaaaaa ttcgattaga cttaaatcaa ttcttaagga aagggtgacc tgtaatgaac
       61 ttatcaaaac gtatttcagc agaaaacaaa aaaaattggc gcagctggcg tagataagtt
      121 gtattaatgg tgagactttc ccatagatac aactttttag agtgcatttc taaagcttgt
      181 atctatgcta gacatgaaca ttgactgccg catagattag aatctatgcg gttttttgtg
      241 taaagacaat gaccgctttg gccattgtct ttttttgtag actggatact tgcctgatta
      301 taataaataa aaagattgac tttgcaggag gaaaaaaatg aaaaacaaac gattaataac
      361 agttgtagta attattttag tttatttagc aggtgcattt atttatggaa aagcctcagg
      421 cgaaaaagaa agtacttcga cgaaccagac aaaaaaaaca acagttgttg gggtattgca
      481 atacgtgagc catccggcat tagatgagat ttatcgagga atcaaagatg gtctgaaaga
      541 agaaggatta aaagaaggag aaaatcttga gatcaaattt caaaatggac aagccgatca
      601 gagcaagcta gccacaatga gtcaacagct agtacaatct gatccagatg ccttgattgg
      661 aatcgcaaca cctgccgctc aatcattagc aaacacgact tcatcgatcc cagttgtgtt
      721 aggtgctgta acggatcctg tgggggctgg attagtcgct tctttagaga aacctggtgg
      781 taatgtaaca ggtgtttctg atcagccgcc tgttacttca caaatcaagc ttgcttctga
      841 tttgttgcca aatgcaaaaa aagtcggaat cttgtatgct tcttcagagg ataattcaaa
      901 ataccaagta gcacaagcag aagaagctgc aaaaaaagaa gggttagaat cagtaaaata
      961 tgcggtgcct tccactaatg agattgcaca aacggttcaa gtgatgagcc gacaagtcga
     1021 ttttatctat gttccattag ataataccat tgccaatgcg atgcaaacag tggtaaaaga
     1081 agcaaacaaa gcaaatatcc ccgtcattcc atctgtggat acaatggtgg aacaaggtgg
     1141 actagcaaca atcgggatca atcaatatca attagggctt caaagcggta agatggcggc
     1201 agaacttgcg agtggaaaag aaaaaccgga aacgacgcct gtttatacat ttgatcaagg
     1261 ggatacagtg atcaatcaat ctcaagctga tcgcttagga atcattgttc cccaatcgat
     1321 gaaggaaaaa gccaaaatca ttacagatga ctcgcagcaa gaaacatcta aggaggatga
     1381 caaatgatcg tatcagctat ttcacaaggc atgctttggg caatattagg attagggatt
     1441 ttcatgactt atcggatctt agactttcct gatatgacga cagaagggtc cttcccatta
     1501 ggtgctgctg tctgtgtgac agctattact catggggtag cgccgattgt agctacatta
     1561 ttaggcgtag gtgctggcat gatagcagga ctcgttacgg ggttacttta tacaaaagga
     1621 aagataccgg ttattttagc agggatttta gtgatgtcag gattgaactc agttattttg
     1681 tttgtgatgc gaacaccaaa tctttcttta ttgaatcatc cgattctaca agacagcttc
     1741 catcgctttg ggttacctga ttactttgac acagtccttc ttggcttcat ctctttgggc
     1801 atcttgattg ctgtactgct attcttcttc aatacagatt tgggtcaagg ctatattgcg
     1861 acaggagaca acgaaacgat ggctcggtcg ttagggataa aaaccgatcg tatgaaaatt
     1921 ttaggtttaa ctttgtcgaa cggtgtcatt gcattatccg gtgcgctgat tgcccagaac
     1981 gacggatatg cagatgtaaa taaagggatt ggcgtaatcg tcatcggtct tgcttccatc
     2041 atcatcggtg aagtgatctt ccaagaattg acgctagcag aaagattgat tgctattgtt
     2101 gtcggcagta tcatttatca gctattgatt ttagtagtaa tcaaactagg gtttgacacg
     2161 acttatctga agttgttctc cgctatcatc ttagctattt gtctgatgat cccgcaattg
     2221 aagaaagcac tgaacttaaa aacgggtttt gagaaggagg catagtaatg actgatatat
     2281 tgacaatcaa aaatggcaga aaagtcgtaa acaatggaat gaatgaagag aaagtactat
     2341 tggatgaaat taaccttaca atcagagaag gggacttcat cactgtcctt ggcgggaatg
     2401 gtgctggaaa aagcacactg ttcaatacga tcgctggtac gcttcaactc agtcaaggat
     2461 cgatctcttt taaagatcag ctgatcacaa aagattcaga agaaaaaaga gcacgattcc
     2521 tttctcgagt tttccaagac cccaagatgg gaacggctcc tcggatgaca gtagcagaaa
     2581 atttgctact tgcacaaaaa agaggtcaaa aacggaaatt ccgtttacgt aacttgaaaa
     2641 atcaaaaaga agattttttc agactatgtc aagaagtcgg taatggccta gagcaacact
     2701 tggatactcc agcgggtgaa ttatctggtg gtcaacgcca agcattaagc ttgctgatgg
     2761 cgacgatcac agctcctgag ttactattat tagatgaaca tactgcagct ttagatccta
     2821 aaacagctaa agcattaatg cgattaacaa atcagcggat caatgaacaa aaattgactt
     2881 gtctcatgat cacgcatcga atggaagacg ccttgaaata cggaaatcgt ctgatcgtac
     2941 tccaaaaagg gaaaatcgtt caagatattt ctgaaaaaga aaagcaacat ttgacaatgg
     3001 gcgacctttt gcagttcttt gaagaagtag tggattgatt tcgacagaaa aattcctttc
     3061 agtagaagga cttgaaaact cattgacatc ttcctttgat tcatcctata cttaaaacaa
     3121 taaacaatta aacatatttg ggtgttttgt aggatcacgt agtcaatgat tttaacaatt
     3181 aaatagaata ttctttgctc tgacaagtaa acgatgagaa aagaatttgc tactttgcca
     3241 cctagacgtt tcgtactttt tcgtatcaaa cgctaagtgg ctttttttat tgaagtaaga
     3301 acaaaggagg aataagatga tttctggaca aaccaaatta gctgggctct ttgctgatcc
     3361 ggcttctcac agtttatccc cattgatgca caatactgct tttgaagcaa acaacatcga
     3421 tgcggtgtat ttagctttcc aagtaaatcc aatgaatttg aagcaagcag tcgaaagtat
     3481 tcgaactttt gatatgttag gtgtaaatct ttcgatgccg aataaaacag cggttattcc
     3541 ttatctggat gaggtaagcc aggaagcgca attgatcgga gcagtgaata cgatcgtgca
     3601 cgaaaagggt cgtttgattg gctataatac cgatggtgcg ggcttcatgc aatccgtgca
     3661 agaagctggg atttcgatca aaaagaaaaa aatgactcta ctgggagctg gaggtgcagc
     3721 aaaggcagtc actgttcaag ctgcgttaga cggcgctgag gaaatcgtag tttataaacg
     3781 aaacaaccaa acattcgctc aagtaaaaga aacattcgaa caactcagct atcgaacttc
     3841 ttgtaagata aaagttcgtg actttgccga tacgcatcaa ttaagaaaag acttgaaaga
     3901 aagctgtctt ctagtcaatg caacagatat cgggatggga aagaaaatca atcagtcacc
     3961 gctcttagat ataagcatga tgcatgagaa tctagcggtt tttgatttga tttatgcacc
     4021 tagagaaaca cgtttattaa aagaagcaaa aaaagtagga gcaaaaggat acaatggact
     4081 tggcatgttg atctaccaag gagcaatcgc gtttgaatta tggacaaagg aaaagatgcc
     4141 gatccatgtg attgaaaagc tgtttgatta aaaaaggata atagataaag atcttttatt
     4201 ctcgcataaa cggtctcaag aaaagtaatc ctttatgtat cgaagcattt atttcgttat
     4261 tttttattaa attatcaaaa aatgataggc ttttgtcgat tggttatcgt atcctttatt
     4321 tatcagtaag aaacgaggag gaaccacaat gagtcggatg gatcgctata aggacatcca
     4381 taagaaggcg aaacctctga agcgggatac ggggagtatt tttgctagaa gagaacgaaa
     4441 acgaacgcgt atggaaaatg aggaagatgc tacccgtatt tatgaaagag agcagcattc
     4501 atttgaacat ttgaaccaat acgagcaaca gatatacaga gatgcaccaa gagcagaaga
     4561 acaacaaaaa aataaaaaat ttttcaatcg gcaaaagaag aacaaaccgt taaaacctcg
     4621 aagaaagcga tcgtggaaaa aaatcattct atcgattttg cttgttttga tcctttattc
     4681 aggtatctca ttttttattg gtaaatatgt agctgaacat gacacaagct tagaggcagc
     4741 gacttcagaa acctttcatg ggcaaaaagc gacgaatggt gcgcataata ttttgatcct
     4801 tggaagtgat acacgcggag aagatgccgg gcgagccgat acgatcatgg tcttacagtt
     4861 ggatggtcct tcccataagc cgaaactgat ttcttttatg cgtgacagct tcgttacgat
     4921 cccaggggta ggacaaaata agatcaattc agcttatgca tacggaggag ccgatctagt
     4981 gcgtcaaact ttagtagaaa attttggaat cgattgtcaa tactatgcga aagtcgattt
     5041 caaatcgttt gaaaaagtga tcgatgcctt attcatgaac ggagtgaaga tcgatgcaga
     5101 aaaagatctg aatctggatg gtgtagatat taaaaaaggg gtccagaaga tggatggaca
     5161 tgttcttttg cagtacgcac gtttccgaat ggatgaacaa ggagactttg gccgtgtcag
     5221 acgccaacag caagtaatga acgctatttt cagccaattg aagaatccat tgaatctgat
     5281 ccgtgcacct tatgcagctg gtaaagcttt aggatataca tccacagatg tcactgcttg
     5341 gttcttgacg aaaaacctct tttcgattgc acgaggagct ggtggcattg atcgaataag
     5401 tgtgccagta gacggttcgt ggaattatgg gcaaagtgat tatgctggca gtattcttga
     5461 aattgataat caaatgaatc aatctgctat tacagatttt ttgaataaat gatcataaaa
     5521 aagatcaaga agaagctgcg caaggctttt gtcatgaatc aatcaaacga ttcactcgta
     5581 aaaaacgctt tcttatggta gatttaacca agagaggagc tgtttacatt ggatatctat
     5641 ctgaacttca aaaatgagtc gcgtgaagcc atcgcttttt atgaaaaagt ttttcagact
     5701 gaagcatcag gtatcaccac atacggtgaa atgcctggaa tagaacaaat gaacttgcca
     5761 gaagaagcaa aaaatctaat cacgaatgca cggctgaaca tttatgatac attagttatg
     5821 tttgcagata cgccagaagg aattggtcct gaattagttg tgggaaataa tgtttcttta
     5881 gtcatccaga caagaaatga agaagaaacg aagcgcctat ttgaaagctt agctgagggt
     5941 ggtaagattg agatgccact ggatgctgtt ccgtgggctc agttgtatgg gatggtaact
     6001 gatcgatttg gcatcaactg gcagattaac caagagtgac agctaaagat gaatattttc
     6061 tggaaacatc aagcagcaaa cggatcatcg tttgtttgct tgatgttttt gtgtctattt
     6121 cgactatacg gtatcttttt tttattgcca gatgcgaaaa tatgttccat aatagagaag
     6181 tccgcaaaaa attaagtaac ggaggtcaga agatgaagac ggtgatacta gctgaaaaac
     6241 cttcacaagc aaaagcctat gccgaaacct tccaacagca aaagcggcat gaagggtatt
     6301 atgaaatcag cgatccattg tttgctggtg aagtaaccat cacttatggt tttggacatt
     6361 tggtagatat ggttgctcca ggagcttatg aagagcgttg ggcaaaatgg tctttagaaa
     6421 atttgcctat tttcccagaa accttccgtt acgaagttcc aatggacaaa cgtgcacaat
     6481 tttcgatcgt aaaacgagaa ttgcagtcag ccgataccat cattattgca acagatggtg
     6541 atcgagaagg tgaagccatc gcgtggtcga ttattcgaca agcaaacgca ttttctaagg
     6601 aaaagaacta tttacgttta tggatcaatt ctcttgaaaa agaagcaatc tatgaaggtt
     6661 ttaagaatct ccaacctgga aagaactatt ttccaaaata taaagaagcg caagcaaggc
     6721 agaacgctga ttggctgatt ggaatgaatg gaagtccatt atatagcttg ctacttcaac
     6781 gaaaagggat tgatggcagt ttttctcttg gtcgtgtaca aacaccgaca ctctatatga
     6841 tttatcagct gcaagaagaa atcaaacact tcaaaaaaga aacgtacttt gaaggagaag
     6901 gtttaattac gacaaacaaa gggcagtttt ctggaaaaat cgtaccaaaa aaagcattta
     6961 aaacccaaga agagttaacg aaggaaatag aaaaacttgg agcgcattta ggtaagcaaa
     7021 acggacggat tcttgaagtt acgaaaaagg aaaagaggtt gcatagtcca cgtttatttt
     7081 cgctatctag tttgcagaca aagatgaatc agctaatgaa agcaagtgcc aaagctacac
     7141 ttgatgctgc tcaaggattg tacgaaagca agtttctaag ctatccaaga acggattcct
     7201 tttacattac agaaaatgag catcagtatc tggttaaaca tctgcaaaat tataaaaaat
     7261 ttttaggaat agaagaagta cagacaactg agatcaaagc aaagaaacga tatgtcgatg
     7321 cgaaaaaagt tcaagaacat catgcaatta ttccgacaaa gacgattccg actcctgcac
     7381 gctttgcgaa gctttccaaa ttacagcaag caatctattt gcaagttttg cgcactactg
     7441 tggctatgtt tgctgcagat tatatctacg aagagacgac cgtaatgacg ggcgtacagc
     7501 aattaaagct aaaaagcatc ggaaagatcc cattacagca aggttggcag gaaatcttac
     7561 gcatcaagtc aaaaaagaaa gaagctcaga ctcttcctcc tatcgtagaa aatgaagaag
     7621 cagaagtaga actgaaaagt atggagaaag aaacacaacc tccaaaaccc tataccgaag
     7681 gaacgttgat cacagcaatg aaaactgcgg gaaaaacgct ggataatgat gaagcgcaag
     7741 aaattctcaa agaagtagaa ggaataggga cagaagctac acgagccaat atcattgaaa
     7801 atcttaagca gaagcattac atcgaagtca ataaaaacga aataactgtt acgccaaaag
     7861 gaatcactct ttgtaaggca gtagccaatg aaccattact gacgagcgca gaaatgactg
     7921 cgcgctggga agggtacttg agaaaaattg gtaaacaaga ggggacacca tctgtttttt
     7981 tagagaatat caaaaaattc atcctacatt tattggcaga agttcctaat caaatcgaca
     8041 aggtagactt ttcagatgag attagcggga cacgtcagat aaaagcgatg gagaagaaaa
     8101 acgatcaatt aggaagatgt ccaaaatgta aaaagggaat agtcatgctt tatcctaaaa
     8161 tcgctacatg cttgaatccg gaatgcgact tcaaattgtg gccaacagta gctaaaaaga
     8221 agctgactaa aacgaatctc agagatttgc tttcaaaagg aaagacaagt aaagttgtca
     8281 aaggttttac tggtaaaaaa gggaaattcg atgcagtttt agtattaaaa gaagatatga
     8341 caatcggttt ttcttttcca tggatcgaaa acagtggaac aaaagcagtt gatggaaaca
     8401 aagaaacatc aagaaaaaat aatgaaaacg aagtagagta aatgaaaaca atgaaatagc
     8461 ggaatattta ttccgaaaag tgagaatata aataccaata aaactaaaaa atatcagtga
     8521 atcaagcaaa aacgttgggg taggctgatt ttatatagat agcaaggagt ttttttattt
     8581 gaaaaaagtg cattcatttt taaattatct tatgggagca atcacgattg tggctgtgta
     8641 ccaagcattt ctaaagtatc ctgattacgg gacgaagttg ttgtcattga cgcacaatca
     8701 aacagcatat tcaccaacgt tgattttatc gactttgact gcaggagcta aaagcgctac
     8761 ttattttttt cataaaacga caagcatgct aaagcgaaag aaacagcgaa aacaattgtt
     8821 ggtggtggta gcggctatga tcattattta ttttctacca gttattacac aagtatttca
     8881 tcaatatttt ggttaagaat acgagagttt gtgacaaaag tcatgtcaca aacttttttc
     8941 tttgtcattt tttcgtaaga aaaagcaaaa taaaagtata tacattgatt aaaagtatat
     9001 acttggatat aatgtactta ctatagaatg agaatggtat agagggtggc gaaatatgaa
     9061 aaaaatgaaa aagtataaaa ttagaaaatc tggcaatagc gatgtaacaa ctatcccacc
     9121 agaagtaaaa gaattcatgg gtgtgcagac aggtgacgca atcagttatg tttttcaatc
     9181 tgatggttct gtacgaatga tcaaagcaca agaagaacct gatattgatt cattagttga
     9241 ttcgatcatg aatcaatatg aagacgcgtt aaaagatttg gttgatttat gaggtatctt
     9301 tcgactaaag aaattatcaa aatcaacgcg aaagttattt tacgtcactc gccaggcgaa
     9361 atgatcggtg taaaagatgc aaatgcatta gatatggccg tcaaacagcc ttcacaagca
     9421 gtattcaatc aagaactcta tcctgaaatc tatgaaaaag cggcgatttt agcaattaat
     9481 ttagctaaaa aacatccttt tcataatggc aataaacgaa ctgctcttgt tgccatgttg
     9541 ttgtttcttc aattgaataa ttgtccagtt gctttttctc gtcaagaagc tgttgatttt
     9601 atcataatga ttaccactag ttcattggat ttcgattcac taaagtccaa gataaccagt
     9661 tatttacgac aaaccgcaat aaaatgaacg aattaaaaac tttatttctc aattagatca
     9721 g
//
LOCUS       DACCXM010000100         9437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCXM010000100 DACCXM010000000
VERSION     DACCXM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.103"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(130..777)
                     /locus_tag="IVY61_002167"
     CDS             complement(130..777)
                     /locus_tag="IVY61_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP7971245.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1056..1559)
                     /locus_tag="IVY61_002168"
     CDS             complement(1056..1559)
                     /locus_tag="IVY61_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7971246.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1682..2446)
                     /locus_tag="IVY61_002169"
     CDS             complement(1682..2446)
                     /locus_tag="IVY61_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP7971247.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2553..2828
                     /locus_tag="IVY61_002170"
     CDS             2553..2828
                     /locus_tag="IVY61_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP7971248.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3505..4455
                     /gene="yidC"
                     /locus_tag="IVY61_002171"
     CDS             3505..4455
                     /gene="yidC"
                     /locus_tag="IVY61_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7971249.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="IVY61_002172"
     CDS             complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="IVY61_002172"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7971250.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(5605..6063)
                     /locus_tag="IVY61_002173"
     CDS             complement(5605..6063)
                     /locus_tag="IVY61_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7971251.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(6060..7523)
                     /locus_tag="IVY61_002174"
     CDS             complement(6060..7523)
                     /locus_tag="IVY61_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7971252.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="IVY61_002175"
     CDS             complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="IVY61_002175"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7971253.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            8598..9347
                     /locus_tag="IVY61_002176"
     CDS             8598..9347
                     /locus_tag="IVY61_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7971254.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT         2804 a         1998 c         1639 g         2996 t
ORIGIN      
        1 aaagaaaggg gctatcccca gactttagta ggcgtcatta gctatctttc gttagcattt
       61 aaaggcgaac accatcacct attcacttcc attaaaagat agcatatttt tctgtaaaaa
      121 acaataaaat tatcgttctt tctttacacg ttcagcaaat tcatagaaag gagccaattt
      181 cgttaactgt tgattacatt ggtcactatc atgacaaata taattcccct ttttcgtata
      241 cgtgccatca gaacctgctt tcgttgtaga aagaaaaagc gacacattcg atgttttctg
      301 acagattgca cagattccct tatttatcgt tggtgataaa gtaccgctaa tcccttttaa
      361 tttttcatct tcgtaataga ccatgaattt tttctgcgta cctggatcat tccagccgat
      421 gtacgtatgt tcttgcaagt cgatttcatt ccaagctggt tgtttgagtt ttttcacttt
      481 tcggaataat ttctcgattt gtggtttgct aggctgcata aacggctgga cgatttgctt
      541 cagctcctcc aatgctcttt ctgcttgcgc ttttgtcagt tttacgtcta aaactgtcgt
      601 taaaaatgta tcgatttctg gcagttcgtg ttctaatatg tctcgaatct tttcagctgc
      661 tagtgcctga actgtttgta ccgtttttgg atcattgaca gatgagtacg catttaataa
      721 ctgatctact tctcttttta taaaaaaata ttggtatggt tttattgttt gttccatgat
      781 tttaaagtcc tttcttctaa ccaaagtgca tactttgtag aaaaaagtga tcttctgcaa
      841 agtatgtgtt acctgaagat cccggactct tttaaataag atacgttttg catcttatca
      901 tcaccccctt taatgttcat tcttctatgt acatgattca ttcgataaat aaaagtaaac
      961 cgtatcttga acagatagtc aactgcaaac agaaaaaacc gagaaaatca gccagatttt
     1021 ctcggaatat agtctattat tattggtttt acctattaaa tcgaggattc tttcccaaag
     1081 taacgttgcc atctcttacg catacttgca gacatgggct gagccgcttc tttgactgcg
     1141 cagtatttgt ccacgaatgt cacaatatat ccttctttat acttaggcgg tgcgatcgtt
     1201 gcaccccaca tgtgcttcaa aatgatatct cgctcaagat ccgacaaatc ggtgattttt
     1261 tctgcattct ttacagcaat tcttggatgg atatacgcat gtgtcccctc gtcaaatttc
     1321 gtcgtacgcc aatcatagta gaacagatca tgcaaaagtc cagcacgcgc tgttgcttta
     1381 gcattgccac cccatttttt tgcgagcaga taactatagt aagaaacact gatggaatgc
     1441 tccaaacgag tggaatgaac atgctgtgtg taattcgcta attttttcac tgcttctgtt
     1501 tctaaaagat cttgtacata agacatatat tcttcgtctt cacgccattt ttctgtcata
     1561 attatcgatc tgccaccttt ttgaatttca ttttcttctg aaattgatct aagtatacca
     1621 ccagtgttcg gattattcta gtcgctctag ataaaatagc taaagcttaa tagttcctta
     1681 atcataaaga aaaatgataa agaacgatcc ctgctgcgac tccgacattc aatgattccg
     1741 catttccttt cattgggata tatacgttct gatcagtcaa agacaagatt tcctgactta
     1801 ctccctgacc ttcgtttccc atgattatag cgaaatcctc tgttttttct actttgtcta
     1861 atgcatgtgc ttcttcattt aattctgtac cgtacaccgt aaaccctttg cctttcatta
     1921 cagggattac atctgccaaa tctttttgta acacaggcaa atgataattg cttccctgca
     1981 tgcttcgcag cacctttgta ctataaatat ctgccgttcc tttacctaat aaaacaccag
     2041 ccataccaaa cgcatctgcc gtacggatca tcgttcccac atttccagga tcctgtacat
     2101 tatctagtaa aagccagcct cctgaaaaat ctggcgtatc ttcagatgtt aacgtcatca
     2161 ctgctaagat cccttgcggt gtaggtagat ctgacatagc ggtcatgacc tcatctgaaa
     2221 ctagaaaagt agaaatctct ttgtttacag ccagccagtc tccccattct ttcatacctc
     2281 tctgtgtcac aaaaagttct tttatttctg cattagacga cgcagcttct tcgatcagat
     2341 ggaacccttc caaaagatat tctgcctttt cttctctgta tttcttttta tgtagttttt
     2401 ttaattcttt gattcttgga ttttttgctg attggatctc tttcacattc ctcacctcaa
     2461 ctgattatat catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt
     2521 gatttataaa acaggaaaga aaggtgacga acatgcgcaa agttaaaatg aatgtccaag
     2581 gacgtgtcca gggcgtggga tttcgctata tgaccaagat ggttgctgat cagttaggcg
     2641 tgaccggaac ggtaaaaaac gaagacgacg gctcagtcac aatcgaagca atcggaaatg
     2701 acgacatcat ccaaaaattt atcgaagaag tcaagaagtc tccgagccca agtggacgag
     2761 tgcagtatgt ggatgtccaa gaagatccga tgattgagga acgaaagaaa tttgatgtag
     2821 tgggataagg ttgtgaaaag gtcgcttagt tttgagtatt aaggaaaatt ttgtgaaaat
     2881 ggctttccac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     2941 tcgataaggt tgtttttgaa gcgctttgcc tcgagtatta aggagaaaca ttttcaaaat
     3001 agcttctcat attttgaaat gtaatgactt aataccgaga ggcagctgat aaaacaccgt
     3061 caataaggtt gtgaaaaagc tggtctgcat caagcattaa ggactggttg tgataaatag
     3121 ctctccatat ttttcaacag gctggactta atgccgtaga tgcaagcttt tgaacaccgt
     3181 cgataaggtt gtttttgagg cgctttgcct caagtattaa agacaggtag taaaaaagct
     3241 gatctataac ttgcaatgaa tttgcttaac ccatattttc cagacagaac aaaaaagttt
     3301 ttagtttttt aaataagaaa gaaacacttg gaatcactct tttctttcta agtatttctt
     3361 tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa aaagaacgaa tatttatatg
     3421 aaatttctat gaagttgatt gaattccatg cgctttttta gtatagttaa gtttgtgtaa
     3481 aaattataga agaggaagta ttctatgaat aaatggaaaa actggttatt gggatccgga
     3541 cttgtcagta tcctgctctt tttatctggc tgtgtgcgga tggattcaaa cggaaaccct
     3601 gacacttcag gaatcgttta tcgagtactc gtccatccta tgggccaagc gattacatat
     3661 ctagttgaga atttcaattg gtcttacggc tgggcagtta ttgcaatgac tgtgatcgtt
     3721 cgtatcatca tcttgccact aggtatcagt caatcgaaaa aaacaatgat ccaatctgaa
     3781 aaaatgcaag cgttaaaacc acaagttgaa gcagcacagc aaaagctaaa agctgcaaca
     3841 acacgtgaag aacaaatggc agcacaagca gaaatgcagc aagtatatcg cgaaaatgga
     3901 ttgagtatga ccggcggaat cggctgtctg cctctattga tccaaatgcc gatcttctct
     3961 gccttgtatt ttacggcacg ctacacagaa ggtatccggg aatcatcttt ttacggcatc
     4021 gatctaggct caccaagtat ggtgcttgtg gccatcgcag gggtcgctta tctgcttcaa
     4081 ggatatattt caacaatcgg tattcctgaa gaacagaaaa agacgatgcg tacgatgctg
     4141 atcgtctctc ctgctatgat cgtcttcatg tcaatcagtg cgccagccgg tgttacactt
     4201 tactgggtag tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg
     4261 aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc aaaacaagtc
     4321 gtcactccaa gaaaagacgt gacagaatca gcaaagccag ctaacaacaa acaagcaaca
     4381 aaacaaatca aacaaccaaa aaacagtaca aacaacaaag gccgcaacgc aggaaagcag
     4441 cagaagcgga agtagatagt aaataagagt gtaataaaaa ttcattaaca gttttagcaa
     4501 ttaaaaatat cgaccaagat ttttgaaaaa gaaatctggt cgatattttt atttaagtga
     4561 atgtttaatt tgaatcatcg attgatcgct atctaattgc agcagcatct ttttatgaag
     4621 aaacaacttt ttcttatcta tcaacgtaga caggtgttgc tgtttcttct aatagtgtac
     4681 ttaattcttt aatattttgt tttccttctt ctaacatcca actactcgct ttttcttcgc
     4741 cttcggtaac aaaaggtgcc actcccttcg cccatgttgc tcgcccacat aagacaccat
     4801 tgaatgtcga acctgattgt tttgcaaaac gtaatgtatc ttgaaatagc gtagcattga
     4861 ctcccgcact taaaaaaata aacggttgtt cagtcacttt cgattgttca tagaaatacg
     4921 cggatgcctc tgcttgacta taacaagtct cttgcccata tccttccaca aatctcatat
     4981 tcactggcac ttctactttc aatacatcca cttgataacg ttcttttgaa aattctcgca
     5041 tggcttcaat aactttatgt ggttttactt ttgcataggc aagagaagac ggatcgccaa
     5101 tagttgcatc ataagaaaga atctctaaaa agaaaggtat gccttctgct tcgcattctg
     5161 aaccaatccg ttctataact atttgttttt gcacattaat ctctgctggt tcatcaacgt
     5221 catagtacaa taaaaattta caagcatccg caccagcttc ttttaacctt ttcaccgacc
     5281 aaatattcaa taaatcaggt aatcttcccg gtattttcgt atcatagccg gttttttcat
     5341 aagctagcag taagccacat tctttctctc gaagcgctgc tgcaggcagg ccgtattctg
     5401 gatcaagtaa aatagctgag gcatagggag tcaggtattg agaaaccatc tgtttaaatt
     5461 ggatgatgtc ttctgatgta gcttctttat attggttgat cattttcttc aaagcaccac
     5521 gctggtcgat tgctaatgcg ctaataattc cagcttcatc cgtcaatcgc ttcaaacgtt
     5581 cctgtttttc tatactcatc ttcttcatag atcttcacct atttctttag ttaatattgt
     5641 ggctatttct gtttttgatt ttgcttttct caactgttct gtaacctctt ttcgcatcaa
     5701 tgatcgtgca acgagagaca ataatttcaa atgagtcgtt cctgcttctg aatctggaat
     5761 aaataacgca aaaataaaat caactgactg tctatctaaa ctgttccatt caattccttt
     5821 gttcagtgtg gcgataatca tactaggttc gttgattgtt tgagacttcg catgagggat
     5881 agcaaaacct gacattattc ctgtagtccc ttctttttct ctttctagaa gtttttgata
     5941 tacttctttt tcgtcaagag cgatccctaa acagaatgtt tgctttgcta aaaacttaaa
     6001 tgcttcttct tgactatcca atggctgttg taaaaaaatg ttctcttcat gaatgattgt
     6061 catgataagt ctcctgctct tcctttttcg ataactgtac gatttgctgc tttattatcc
     6121 atcttcttgt ctttatcagt tactcttttc ttttggatta atcctagtat cactccacca
     6181 acgatcgaac caataagaat cgctgtaacc cattggattt ttcccgtcac tacaggtaac
     6241 actaaaaaac caccatgtgg tgctggaact tggacgccaa acaaataagt taagacagca
     6301 gcaattgatg agcctaacat catcgtgggt aacactcgga tcgggtcttt cgcagcaaac
     6361 ggaatagctc cttccgtaat atgagttgat cctaaaataa aattaaccaa acctgcatta
     6421 cgatcattgg tatcaaaata tttaccaaac aataaagtag caaaaccagt aatcaacggt
     6481 ggtgcaatac aagctgctga cacaccagcc ataaaagtcg tgtttccttg tgccaaaaga
     6541 gctgttcctg ttacatatgc cgccttatta accggtcctc ccatatcaaa cgcacacata
     6601 cagccaacaa tcacgccaag aacaagtgga ttcgaatgtt caaatccagc taaaaagctc
     6661 atcattcctt catttatagc cttcattgga cctgccagca aagacatcac gatgccaata
     6721 aatgctacac caataactgg atataagaag attgccttca agccatctag ttgtttaggt
     6781 aaaattgtca gtagcttttg caaaaataag ataaaataac ctgctaagaa cccagctaaa
     6841 atacctccta aaaatccagt accgccattc atggctacga ttccaccaac aaatccaacg
     6901 actaaccctg gacgtttcgc gatcccttct gcaatataag ctgctaaaac tggaaccatc
     6961 attcccattg ctgcatcgcc aactgctttt accattgccg ctgtagcatt gtattgtgca
     7021 tgatctggat caaaagaata gattccccaa acggcaaatg aaatagcaat caatactccg
     7081 ccagctacca cgaaaggcaa catatgagag acaccgttca ttaaattttt atagatgcta
     7141 cgaccaaaat tagcaccact catcgcttct ggttctaaag tatcagcagc ttcattatct
     7201 gctaagacag taccttttcc tgccaatgct tcgtcaatca gccgttgtgc atccttgatt
     7261 cctgtactga cagaaacatc aatgactcgt tttcccgcaa atctttctga atggacatct
     7321 ttatctgcgg caataatgac agcatctgcc tgtttgattt cttcagccgt taattcattt
     7381 tcgatcccaa tttgtccatg ggtctccact ttgatcttga tgcctttttt cctagctgct
     7441 tgttctaatg cctcttgcgc catgtaagta tgcgcaattc ctgtcggaca accggttgct
     7501 gcaattagtt gatattttcc catgtttcaa acctctcttc cttgattttg atttgtttac
     7561 ttaattcctc cacatccaaa aaatcagtca gtcctttacg aaaagcggtt gagctgcctg
     7621 ctgctatgct tttacgtaac gtttcatcaa gcggtcgtcg attcatatat cctgctaaaa
     7681 aagcgcctaa catggtatca cctgcacatg ctgtattgac aacttttcct tttggcgagt
     7741 ttcctcgaaa caccctgtct ttcgtaaaaa gaatcgctcc tttctcaccg agggataaca
     7801 aaacattttc tgcacctaac tctactagtc gttgtccata aacaaagtaa tcttcatctg
     7861 taaccatttt tcgtccgaac caagaagaaa gttcttcctc gttcggtttc agcaaaaacg
     7921 gtcgataggg taaacaatcc atggcttctt gatcactact atcaataatc aaatgaatcc
     7981 ccttttcctg acaaatccga ctgatttcta ttaaaacctt tggagacagc cctcttggta
     8041 aactcccaga aacacaaaga taatcttctg cttgtaatgc tcttatttga tttaaaaaat
     8101 tatggacagc tgtctgcggt attttaggtc cttgattcac taatttgtat tcttctcccg
     8161 tttgattgac ttgtgtaaat acattgatac gcgtcatacc aggcacttca ataaactctg
     8221 tttggatttg tttctgctgt aaatagtctt ctatatactt tcctgtaaac ccagcagaaa
     8281 aacctaatgc cgtacttggg aaatctagca tttttaatac tagtgacaca ttcacccctt
     8341 ttccgtttgc ctgaatgtca tcctcttttg tgcggtttac cataaaagga tgcatctcct
     8401 ctgtttcaat aaatagatca attgccaaat tcatcgtgca tgtgtagata gacataacca
     8461 acgacctcct tgactctatt atgaaatacg cgaaagagaa aatgttttct taatcgtccc
     8521 aacacaaaga aaacctttac atttataaac tcgtcgtgct attttttaac taacttttgg
     8581 tgaaagaagg aaagaatatg gatcacaaat taagtgaaac agaacagtac ctctggaact
     8641 ttatcgaaca ccacatccta gagatcccta actactccat cgtgaaacta agtgaacaag
     8701 caaatgtctc aacagccacc attgtgcgga caatgaaaaa gaaaggctat gaaggattta
     8761 cttcgtttaa gcaccatcta aaagaaggag aaaacaagaa catcaatttt tctttcttag
     8821 ataaagtaga caaaggtatc cgtcgagcca ttctaaaaaa tgaacaagaa gtggtacgta
     8881 cgatcaatat gttggaaatc ggaaatattg aggatgcaat acaaagaatc aaatttgctg
     8941 accgggtttt tgtctttgca cgaggatttt cagaaatgat tggtcaagag atgctcgtaa
     9001 aacttcaatt aacagggaaa aattgtcaaa tgcatactga tccagaaatc atcaaaagca
     9061 tcagtcggaa actaactaag aaagacagcg ttatttttgt ttctttgaac ggcgagacaa
     9121 aagaactcgt caccgccgca aagaattgct atgatgcaga gattggttcg attctagtta
     9181 cagccaatca ttcctctaca ctgaaagact attgcgagat caatctcgta ggtttcaaat
     9241 cagaaggttc ttatttccct gattatgaag tacggtcacg tttgcctctc cagattatag
     9301 cgagaatcct gcttgacgcc tatgtattgc gtatggggga gaattgaaaa gcagtttatt
     9361 atggttggtt acagtttaaa acatcacatt ttttattctt tatattgtat ggactcacgg
     9421 aaacttttta tgtataa
//
LOCUS       DACCXM010000101         9437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCXM010000101 DACCXM010000000
VERSION     DACCXM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.104"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            33..1007
                     /locus_tag="IVY61_002177"
     CDS             33..1007
                     /locus_tag="IVY61_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7971255.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            989..1450
                     /locus_tag="IVY61_002178"
     CDS             989..1450
                     /locus_tag="IVY61_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7971256.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1500..1964
                     /locus_tag="IVY61_002179"
     CDS             1500..1964
                     /locus_tag="IVY61_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7971257.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1978..2727
                     /locus_tag="IVY61_002180"
     CDS             1978..2727
                     /locus_tag="IVY61_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7971258.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2727..3557
                     /locus_tag="IVY61_002181"
     CDS             2727..3557
                     /locus_tag="IVY61_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7971259.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3557..4204
                     /locus_tag="IVY61_002182"
     CDS             3557..4204
                     /locus_tag="IVY61_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7971260.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4302..5078)
                     /locus_tag="IVY61_002183"
     CDS             complement(4302..5078)
                     /locus_tag="IVY61_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7971261.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5222..6181
                     /locus_tag="IVY61_002184"
     CDS             5222..6181
                     /locus_tag="IVY61_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7971262.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6298..6606
                     /locus_tag="IVY61_002185"
     CDS             6298..6606
                     /locus_tag="IVY61_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7971263.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6633..6932
                     /locus_tag="IVY61_002186"
     CDS             6633..6932
                     /locus_tag="IVY61_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP7971264.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7106..7657
                     /locus_tag="IVY61_002187"
     CDS             7106..7657
                     /locus_tag="IVY61_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP7971265.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7803..8096
                     /locus_tag="IVY61_002188"
     CDS             7803..8096
                     /locus_tag="IVY61_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971266.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8089..8385
                     /locus_tag="IVY61_002189"
     CDS             8089..8385
                     /locus_tag="IVY61_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971267.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8456..>9437
                     /locus_tag="IVY61_002190"
     CDS             8456..>9437
                     /locus_tag="IVY61_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7971268.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMT"
BASE COUNT         3326 a         1363 c         1778 g         2970 t
ORIGIN      
        1 ttataatact tattagagtg agggataaag aaatgagaca actacacaaa gaacagattg
       61 aaacagcaat ggcagcgtta aaggaaaaga caattgatca tgtatatttt acagcatgtg
      121 ggggttcaca agccgtttta atggctggtc aatatctatt tgataaagaa ttaactgtac
      181 cttgtcatgt ttatactgct aacgaattta actatgctat accaaaagga ttatctgaaa
      241 attctttagt tatcacttgt tcccattctg gcacaacacc agaaactgta gaagcaacaa
      301 aaaaagcaag agaatttgga gcaatgacta tttgtttatc taatgaagaa ggatctcctc
      361 tatgggaagc agctgaatat cctgttcact atgattgggg taaagatgtt gatgctagtg
      421 atttaaataa aggaattctt tatggtttat tatttaatat tttacttgtt ttagatggct
      481 ctaaaaaatg ggaaacttgc ttgaatgaat ttgataagtt aaatgatctt gttattgctg
      541 aaaagagcaa atttgaaata actgctaaag aatggggtaa aaaccaaaaa cgagataaaa
      601 ttatttacac aattggtagc ggtatcaatt acggagaaat ttactccaca gcagcttgct
      661 ggttcatgga aatgcaatgg attaattcta atgctattca ttctggggaa tttttccatg
      721 gtccttttga aatcactgat tatgatgtac cattcatctt agtgaaaagt attggagata
      781 caagattttt agatcaacgg gtagaagact tcactaaaaa attcagtaat aaattaatgg
      841 ttttagacca agctgatttt gacttagaat ctatagatag tgtagcgaaa gaatatattg
      901 cagctgtact tagtggcgtt gttattcgcc aaatggtaga agcgattgct tttgaaagag
      961 gccattcctt agatgttaga cgctatatgt ggcaaatgaa atattaatta ataggaggtt
     1021 gcaaaagatg gcaaatcaat tatttcacat tgtgattaca acacattcta ccctttgtga
     1081 gggttattta aaggcaacaa aattaatttt aatgaatgac gccacaggta ttagtgctgt
     1141 tccatttaaa gaaaatacta gtccagatga ttttgaggag agtatgaaag aaataattag
     1201 tcagcatcat ttgcaacctc taattatttt aacggattta attggaggaa cacctaataa
     1261 tgtttctatt aaacagttga acaacaatag aattcaaatt attgctggga ttaatttacc
     1321 tcttttactg gagttattaa tggcccagtc agctggtaaa tctatagaag aaatagattt
     1381 taaggaatta ctgaataact ctcaaaaaag tatggttcat attaattcgc ttttaaaaga
     1441 tgtcagttaa ggtggcaatt tctattctat aaattttaaa aattgaagga ggaaatagaa
     1501 tgattaaatt agtgcgtgta gaccacagat tacttcatgg acaagtaata ttttcttgga
     1561 ctaaacagta tgatattaac catattattg tggctgataa caaagttcca ggagatccaa
     1621 tgtctgtgat ggctttgaca attgctaaac ctgctgattg caaattagac attgttaaaa
     1681 ttagtgatgt taaagggatt atggaaacat ccccaaatga taattacatg attttaatta
     1741 aaggacctaa ggaagctttt gaattagttc aaaatttacc agaaattaaa gaaattaatt
     1801 ttggcggtgt gtctaagaaa agcgagtcca aacaatatgg aaaggcagtc tttttaactc
     1861 ctgaagaatt agaagccaca aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac
     1921 aagtcccatc ttcaagtatt gaaaatgtag attttagtaa gtaagggggg aaaataaatg
     1981 ttattaaaag ccattttatt aggtttagtt ggtgtggtag ctgttattga ctccaggtta
     2041 attggtcgac aaaatattgg tagaccacta attttgagta cacttgccgg acttgtgcta
     2101 ggtgacttgc atacagggat tgttcttggg gcatctttag aattaatttc tatgggattc
     2161 gtctcaattg gggcggctgg acctcctaat atgcaacttg gtagtattat tgccactgct
     2221 tttgcaattt taactaatag tagtacagaa acagccttaa caatagctat cccagtggct
     2281 gtagctggtg aatttatgag tattatcatg cgcatgataa ttgctcaatt tggacatgct
     2341 gcagataaag ccattgaaaa taataaattt agaaaagctc aaattattca tatttattgg
     2401 tcatttattt ttaatgcttt tgtttacttt attccaatct ttttaacagt atattttggt
     2461 gctgacgttg tggcagattt agtagataaa attccagaaa tgattacaga cgccttgact
     2521 gtctctggaa atatgctttc cgcattaggg tttgcaatgc tattaagcac aatgttgagt
     2581 aaaaaattat atccttactt tatttttgga ttctttattg ttgcctattc aggtctttca
     2641 ctaattggcg tgacaatttt tgcagctctg attgcctttg taatggatca agttaaatat
     2701 ggaaaaagag aagaggcgca tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca
     2761 caaaaaaaga tcttaataag atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt
     2821 atgaaagaca aatgcatatg ggatttatgt atgggatggc gccaatttta aataaagttt
     2881 ataaaaatga tgaagagaaa aaaagagaag cttatactcg tcatatggca ttttttaact
     2941 gtacgcctca aatgacttca tttattatgg gactttgtgc ttctatggaa gaacaaaatg
     3001 ctcaagcaga agaaggaaac tttaatactg aatctatctc catgattaaa accagtttaa
     3061 tgggtccttt tgctggaatt ggtgattcat ttttccaagg tactttaaga attattacat
     3121 ttgggattgg attgtctttt gctaagcaag gtagtatttt aggaccgatt ttagctgttg
     3181 gcctttttgc tataccttct attttagctg ttttttacgg cggtaaatta ggatatgagt
     3241 ctggcaataa ataccttact aaattgtatc aagaaggtct tatggatcga gtaatgcaat
     3301 ttgcttctat cattggtttg gctgtagtag gtggaatggt ggctagcatg gtgccaattg
     3361 tcaccccttt aaaattcact acagggggaa cagaacttgt tttacaagac ttacttgatt
     3421 ccattattcc tggattatta ccacttgtat gtactttggg aatatataaa ttgattaaga
     3481 aaaatgttag tacaaatatg ctcttattag gttgtatttt ggcagggttt gttttaagta
     3541 taataggtat cttataatga ttaaggctct aatttttgat atggacggag ttttaattga
     3601 tagcgagatt atgtatcaaa agtgggtatt taatttttta aaacaagaaa atttaagcta
     3661 tcctttgtat tcttatcaaa aaaagattgg aactacagca agtatttttg atgatataga
     3721 aaagtacaat gagggatgta acagtttaga attgaaggga aaatttacta aatattggaa
     3781 atcgcaaaag attgattact tggatatttt tcctaatata ctaattgaac agcttgaatg
     3841 gttaaaaaag aaaaaaataa aaatggcaat tgcttcttcc agtccaatgg atactataaa
     3901 tgaagttgtt accacttgta aaatagagtc atattttgat tgtttaatta gcggaagaga
     3961 tttgccagaa agtaaaccta atcctactat attccttaaa gcttcagaac agctcagaat
     4021 tccagttgaa gaatgtctgg tgatagaaga ttcttataat ggtataaaag ctggtaaaag
     4081 agcaaatatg aaggttcttg caattaaaga taaacgtttt tctcaagatg taagtctagc
     4141 agatgatgtt atttacgata ttaaatatct gcgacaagaa gtacaggtga aatttggaat
     4201 ataaataagt tttttgatgt atttagagaa tattagtaaa aataaaaatc atgcccaaat
     4261 ctaatcaaaa ttgattgctt tgggtatgat ttcttttaat atcaatctat tactttagga
     4321 tctgaatcat ttttatgcac cgtatctaaa gtgatatttt ctgtgacttc aataacataa
     4381 ctaattctat cacaacgaac tgtggattga gaataatgaa atggttgatt atttttatct
     4441 ttgccaacct tttctaaaat aaatagcgga tctcctacta tacattgcaa taagtcggaa
     4501 agctcaggtg ttgcagataa accgtttatt tccataatag agctcgtagc tttgacatca
     4561 tagtcatctt ctagaagttt atataatgaa gtatattggc tacctttttc aaataaagtt
     4621 ggaaattttt cttctgatgc gaaaaaatta tcaattccca tcggggcacc gtcttcccac
     4681 attaaacgtt taatattgta aactctatca gttacattga tttgtaattt attagctaaa
     4741 aactcatcag cctttataat tttagaatgt aaaacagtat atctaatttt atgtcctcgg
     4801 gtagaaagag aatcgctaaa tccatgaact ccagctaatt ttaaactttc ttttttttga
     4861 gttacaaagg tccctttccc ccttaatcgt tcaactgttc cactttctgc taaaagttta
     4921 aaagcttttc gaatggtaat cttacttact ccataaattt tacataattc ttcttctgta
     4981 ggtaattgct ctcctggaag atattttttt tcacgaatat cgttacgtat agaagcttct
     5041 acttgtaaat ataatggtct attatcttga aaattcatag tacactccct aaaaagttat
     5101 acttcattat atcacaattt attaaaacgt cgattgtaaa attaagctag acaactaaaa
     5161 agatcatttg tgctacactc aaaatagttc cgaccaaaga attaataagg agtgagtaca
     5221 aatgacttac acccatctta caacagacga actagtaatc atagagtctt atttcaatat
     5281 cgagaaacct gtcgctcttg tcgcacaaag tttaaatcgc tctagacaaa ccatttataa
     5341 tgtttattcg tttctcaaac aaggaaaaaa agtgctagat tattaccgtc aatacaaaca
     5401 aaataaatct cgttgtggac gacgttcaac cgttttatct gatgaacaat caacatatgt
     5461 tcaagaaaag gtagctcaag gatggtcacc cgatgtgatt atcggccgtg gcgagtttcc
     5521 aattgattgt tctgttcgta cactctatcg gatgtttaaa aaaggccttt ttgacacaca
     5581 gtcgttacct atgaagggga aacgtaaacc gaataatcat caagaaaagc gtggaaaaca
     5641 ggcctttcgc agaacgattc atgaacggaa cagtctctat ccacacctga ctaaagaatt
     5701 tggtcatctc gaagtcgaca ccattgtggg agttaaacac aagagcgctg ttattacatt
     5761 ggttgagtgt ttatccaagg ttattattac tttgaaaccg acaggcagac gagccgaaga
     5821 tatcgaaaac aggttaaatg attggttcaa atccatgcct aaacacttgt ttaaatccat
     5881 gacatttgat tgtggaaaag aattttccaa ctggcgatct ataagtaact cgaacgatat
     5941 tgccatcttt ttcgcagatc caggaacacc ttcgcaaaga gggctaaatg agcactcaaa
     6001 cggcttgctt agacgagatg gcttacctaa aacaatggac tttagggaca cggatgaaac
     6061 ctttattcaa tccgttgcct cgaaaagaaa ccatattcca agaaaatcat taaattatcg
     6121 aacaccattg gaagtatttt tgagctgcat agataaagac atattgtcta gcttaatttg
     6181 acaaatcaga tagtataaaa tgaatcaaat tgaatatcaa atatatatta tatccatgaa
     6241 gcccaatctt ctttaataga agattgggct atttttatgg caaaggaggg aaaagaaatg
     6301 tttcaaaata aaggacaacg ttatgtcaca aagggagtaa tggacagttt gccggtggaa
     6361 ttacaagctt tatgttggaa tctaatcgat caaaacgttc aaaagcaatt tcatttagac
     6421 tatcttcaaa tctttgaatt ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa
     6481 gaagaaccag aagcaagaaa agagtatctg atttcaccta agcttcgttt gaaatcagtg
     6541 tctcaaaaaa tatgggtcat tgattcgggt gagtatcaaa cgatgttatt accagaagaa
     6601 tactgatatc taaaagaaag gaacgaaagg acttgttaga aacggctatc gaagtactgg
     6661 aaaagtgtgc acagctagtc actactcctg aagagtgggg ctatgaatcc gttacgatgg
     6721 aaaaagagga aattgaaatg ggtatgcttc ctgaagatgt tactctacca cgtttagtga
     6781 tgacacatct ttatatttat tgcgcaccag aagatggaaa agactatgta gtttatttca
     6841 ttacagatat taccagccag cgagaatttg ttcgtgggtt actggtagaa ggacgattgg
     6901 tttggagtca gattggagga acgaatgaat aagatcgatt atttgagaat gattgcgaaa
     6961 tgtttagaag atatccttac agcgattcat caaattctga agaaaaaagg agaaagaata
     7021 tgaaatattt tcaactagta ttgacactta taacggcaat ggtgattgaa ttgcttgaac
     7081 aaaaattagc ataggagaaa aagagatgat tgaaaaagaa ttagtgacca cacaaacaga
     7141 agtttggcga gatcctcatc atacccatcg ctatgttttc aaacgacaat gggcgatcaa
     7201 aggaaaagaa gcggaagata aattagcggt agtcattact attcgtccaa cagatacaga
     7261 accatttacg aatgatttga gtatgttatt aatcgaaaag aatatccgac aattaggatt
     7321 tactggtttt attgctgtga atcttttttc gtatacaaaa gcgaaaagtc ctcgggtatt
     7381 tcctcgtgga aatgatgaac aatctttgga aatattaaca actgttttca cagaaaagaa
     7441 aatcaacacc attatttttg cttgtggttc gattacagcg acgagtcaag tggcttatga
     7501 acaagcgaag accatttacg atcaattgag tgcgaaacaa aaaaagatga ccaaagtatt
     7561 gataaatgca gaaggtaagt tatcccaccc actaagtatt catgttcgta atgaatggca
     7621 attggcagat agtcaattat tatttcaaga aaaataacat tttattttct tatgttactt
     7681 ataagtagtt ttacatgctt tttgacttta aaaatacatg tgatgataca tgttatatga
     7741 attatcaatg tacccagtaa tatgctattt tcattatgta aaaaataaca gggggtgtgt
     7801 agatggagat tgatgaaact tgtttgagta actcttataa aagtatgaat gacttttttg
     7861 aggaaatgga actggattca gaattgctct atcaatttta tcttgctttt cgagggcaac
     7921 agatttcttt tccaatgaaa atgtacgatc gtgagttagt tagaaaaaga atcgaaaaca
     7981 tgatagaaca ggaaaaaact gtagatatcc gtcaattgac agaagtttat ggtttctctg
     8041 caagatggat tcaaaaagta attaaacaaa aagaaaggag acgagtacat ggctaagctg
     8101 attgtattag gtggtttaat ttacggtgga tattggttat ataaatatat gcttgcgcat
     8161 ccgaatatag ataaaggaaa agtaattaag acagttgggg taatcatagt tggactcata
     8221 gctctaacta agaaaaataa tactaatagc ccaaaaataa tgagaactta tcgctgtaga
     8281 ggatgtggaa gggaaatttc tactagtttt aatccatctg aaagccctca aggaagagat
     8341 cgttgtcatg ctggctatcg acattcttgg gaaaggatta actaaatgaa caccattgag
     8401 attaattgac tgataaaata agtacgcgag ggagaaattg aaagggggaa ataaaatgaa
     8461 aaaatggttg ttattactaa ttggaatggg gattttattc actggttgta catctgagaa
     8521 tacgaggact aaagagactg tagaaactga tacaacaatt gaggctaaaa aaagtaaaac
     8581 gcaatcaact gaacaaacta caacagaaat gaaacaatct gtggaagcaa gtacaagtga
     8641 aagttcaatc attgaatctt cttctgaaga ggttaaaaat actttgtggg atagtgagaa
     8701 atctactcaa ctagcaattt ttgtcccaca atggggaaag acattaggtc aagaatataa
     8761 aagctacaat cctcagaaca atgtaagtct gtatggtacg cctttaccaa gtgcagttat
     8821 tgatggaaat tggaaaatgg caatcaatga agcaccagtt accgtacaat ggtcagaaga
     8881 tggaactggt catgcagatt tcaatttagt agcagtttat tctgatgtag aaactggaga
     8941 atatttaggg cagcatgtat atttctttgg ctttcaaaat ggacaaccta aagtttattt
     9001 aacgcaacaa aatcaaggca atgataaaaa ctacatgtac ttcaatgaaa ctcagaacca
     9061 acagttaaaa caaggtttca ttgatattgt gaatggacaa actcctcaaa ctccagtagt
     9121 agaagaatct actcaacaag caacaactac aacgacacaa tctacaagta gctcagcaat
     9181 ttcaagttgg gaagctgcta aagaaactgt actaaataat aaagctttat ggttgtatga
     9241 ttctcctgat aaaaatgcat caaatactaa tttaattagc acaaatgaat atatgaaaga
     9301 tctaatgcaa gatgaaaatg gaagctacta ttcaattaat attgtaacgg atgataatgg
     9361 aaatacaaca atggctggtg gaaaacaatt ccgtgtttat acagatggaa gaatttctca
     9421 acgtattgga atgacta
//
LOCUS       DACCXM010000102         9344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCXM010000102 DACCXM010000000
VERSION     DACCXM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.105"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            6..653
                     /locus_tag="IVY61_002191"
     CDS             6..653
                     /locus_tag="IVY61_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP7971269.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            737..1681
                     /locus_tag="IVY61_002192"
     CDS             737..1681
                     /locus_tag="IVY61_002192"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP7971270.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1816..3267
                     /locus_tag="IVY61_002193"
     CDS             1816..3267
                     /locus_tag="IVY61_002193"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP7971271.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3288..4124
                     /locus_tag="IVY61_002194"
     CDS             3288..4124
                     /locus_tag="IVY61_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7971272.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4124..4783
                     /locus_tag="IVY61_002195"
     CDS             4124..4783
                     /locus_tag="IVY61_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7971273.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4909..5598
                     /locus_tag="IVY61_002196"
     CDS             4909..5598
                     /locus_tag="IVY61_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971274.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5604..6560
                     /locus_tag="IVY61_002197"
     CDS             5604..6560
                     /locus_tag="IVY61_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP7971275.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6604..7116
                     /locus_tag="IVY61_002198"
     CDS             6604..7116
                     /locus_tag="IVY61_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7971276.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7372..7920)
                     /locus_tag="IVY61_002199"
     CDS             complement(7372..7920)
                     /locus_tag="IVY61_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7971277.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8009..9190)
                     /locus_tag="IVY61_002200"
     CDS             complement(8009..9190)
                     /locus_tag="IVY61_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP7971278.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         3104 a         1708 c         1877 g         2655 t
ORIGIN      
        1 aatcaatgaa aaaaactgtt atttatacta gtttatttgc agctttgttt ttagctgctt
       61 gtcaatcaaa taccaatcaa ggcacatctg ggaatacgtc ttccagtagc gaacagcgct
      121 cttcacaggt atcggactcc tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa
      181 gttctagtga agcaagtact cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat
      241 tgttccaaga taaataccca gatactgcaa ttactagttt ggaattagat agtgattggg
      301 gatcttactt ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg
      361 atgctcaaaa tgaaaaaatg gagacagaga atccagaaac tctagatcga gatgaacaaa
      421 atggtcaaaa gaaaaaagaa gatggtctgg atgttgataa attgatcacg attgaggaag
      481 cagcaaaaat cgccgtcgac aaagtaggaa gcggtacggc aacagattgg gatttggata
      541 aagatttagg tactacttat tgggaagtca aagtaaaaga cggcaatcaa agtacatcag
      601 tcaagatcaa tagccaaaca ggcgaagtac tgtcaactga acaagatgat taagaatttc
      661 ttcggaaaac aagagttgat ttttctttta gtcatgatac actagacaaa ataacttgtt
      721 tgaaggagcg atttacttga gtttagaaac gacctttgcc aatcatatct ttgccaatcc
      781 tttgatgaat gcatctggtg tccactgcat gactacccag gaattagatg aactggctca
      841 ttcagaagca ggagcattta tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc
      901 cgaaccacgc tattttgatg ttcctttagg gagtatcaat tctatgggac ttccaaactt
      961 aggtttttct tattatctag agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa
     1021 tcaaccgtta tttttttcca ttgccgggat gagtgtccag gaaaacttgg aaatgcttga
     1081 gaaaatagaa aaaagtgggt ttaatgggat cactgaatta aatttatctt gtcccaatgt
     1141 gcctggaaaa ccacaactag cttacgattt tgaggccact tatgaaacac tgaaagaagt
     1201 attcagcatc ttttctaagc cgctaggaat caaattgcct ccttattttg attttgctca
     1261 ttttgatcag atggctgata tcttgaatca gtttccattg acctatgtca atgctatcaa
     1321 cagtgtgggc aatggtttgt atatcgatac agaacaagaa gcagtagtca tcaaaccaaa
     1381 agaagggttc ggcggcattg gcggagagta tatcaagccg actgctttgg cgaatgttcg
     1441 cgctttttat acacgtttga aaccagaaat ccaaatcatt ggtacaggtg ggattcggac
     1501 aggacaagat gcatttgaac atctgctatg tggcgcaagc atgttacaga ttggtacaga
     1561 gcttcataaa gaagggccgg aaattttttc tagaatcata aaagaattaa cgcagattat
     1621 gtcagaaaaa ggatatacgt caattgacga atttaaaggc aaattacgca ccatttcata
     1681 gcaaagagac gaaggtcatg attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt
     1741 cattcctgtg caagcgtact cattgtatgt tagacgaact tgtgggaaaa taacagaggc
     1801 aaaaaggagg ataatatgaa tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa
     1861 tttagttttc aagaactagt tacggatata cagaaaaaaa taaagaagca aaatcatgaa
     1921 ttgaatgcgt ttgtcgcgct tgaaccattc gagagtaatt cccacgagct attgattccc
     1981 cagtcagaaa aagggccatt ggctggtatc ccatttcctt taaaaatgct cggtcaagaa
     2041 aaaaagggat ggctagctac ttcaggatct cgtttatttg aaacacaccg agcttctcat
     2101 acatctaatt atgtgcggca agcagaagcg ataggtctag ttccttttgg acaaaccaat
     2161 gcgccagagt ttggtttcaa aaatattaca gacccagtca tctatggccc agcaagaaac
     2221 ccttggaacc ttgaatgtac accgggcgga tcaagtggag gagctgctgc tgcagtttca
     2281 tcaggaatcg ttcctttagc tggtgctagt gatggcggtg gttctattag aatacccgct
     2341 tcgttttgcg gattgattgg gctgaaacct tcgcgaggga cgatgccagt tggtccatat
     2401 gcttggagag gatggcaagg agctgctatt gatttcggct tgacagtttc tatgagagac
     2461 acggaggctt tattttatgg gatgagaagt atccattccg gtgcgccata tcaagtatcg
     2521 ccagttgagt ggcaaacaca tccaagaaaa aaacggttga agatagcttt ttgtgttgat
     2581 tcaccgatcc actcacagat tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat
     2641 ttccttggaa aagaagggca tgatttgaca gagattcctt acccactaga tggtcgtaaa
     2701 ctgatcacgt cttattatca gatgaatggc gcagaaacag cagcaatgat ccgctcgatt
     2761 gaaacaggca tccagcgagc tgtcagaaaa gaagaacttg aacccatttc ttggactttg
     2821 ttccaatatg gtcagaagct ctctgccgct acttacatcg attctctgca tgtatgggat
     2881 gaagcagcaa ttgtcatgga aaacctacat caagaatacg atctattcct ttcaccaacg
     2941 actgcattta ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag
     3001 atggaacaca tcagtgattg ttctgaagta gaaggtttag acgttgtttc ttccatgttt
     3061 gaggagagct tgaagatcac accttataca cagttggcta atctgactgg acaaccagca
     3121 atcagcttgc ctactcattt gacaaaagaa aacatgcctc taggaatcca atttatggct
     3181 gccagaggaa gagaagattt gctatttcaa atcggctatc tatttgaagc aaatcatcag
     3241 ttccatttac caaatccatg gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa
     3301 gattgtttgc tctaataact agtgcctttt ttattttaag cgcttgcggt acagctgccg
     3361 ctaatcagga agataacagc tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga
     3421 cttctggcac actgtttccc gcatcttatt ataatgatca aaatgaatta gttggttatg
     3481 atgtagattt ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc tttaaagaat
     3541 acaacgtgga tggacaaatc acttctgttt caaaaagaga atcagatttt gctgcgaacg
     3601 attttggtat cacaaaagaa agagaaaaaa aattctccct ttcagagact ttcaaatatt
     3661 catttgacag tatgatcgta cgaaaaaaag atgattcagg aatccactca cttgaagatc
     3721 ttaaagggaa aaaagcagct ggcgaaccca atactagcta tatgcgttta gcggaaaaac
     3781 acggagctaa attagtcaca tatgacaatg ccacgaatga tcagtattta agcgatgtag
     3841 caaatggtcg aacagatgtt attctaaatg attattatct gcagaaaatg gccgtatctg
     3901 ctttaccaga tatccctgta aaaatactgg agaacgttta tttcaatgaa aacgaaagtg
     3961 gttttttatt gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag
     4021 agatgaaaga agatgggacg acaaagaaaa tatctgaaaa atattttcaa actgatgttt
     4081 cagtcaaacc gaaagaaacg atggctgagt cggtgagtga gtgatgtact ttcctctaat
     4141 tgatttttcc ctcatgtgga cttcacttcc tttcgtactg caaggcttgg tatacacatt
     4201 agggattggc ttcgtgtcgt ttcttttagg aaatttggta gggttgcttt tgacagtttt
     4261 aggtcttttg gattggctgc ctctgaatgt atttattcgt ttttatttat cgttctttag
     4321 aggcattccc gcactagtcc tgctgtttct gttatatttc gggttacctt atcaattaag
     4381 tgccttaacg gcttcagtca tctgctttac gattaccagc agtgcattta ttggtgaaat
     4441 ctatcgagga tcactagcag gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg
     4501 tttttctttc tttggggtat tgaaatatat cgttttgccg caagcttttc gaatctcagt
     4561 accggcactc agtaacgtag ctatggatct agtgaaaggt acttcgttag cagcaacaat
     4621 cacagtgcca gaaatatttc aaaaagcaaa gatcataggc ggtcggacat tcgattattt
     4681 ttctctctat attttagttg cactgattta ttgggtcatt tgtatcggga tcgaaaaagt
     4741 gcaacagtat ttagaaaaac gttctatcca tcagtcaatc tgaaaaatag aaatggtgct
     4801 agcgtggctg gcaccatttc tattttttgt ttcttgtata ttctgcctag tattttaaaa
     4861 ccatttcatg taaaatgtaa atcgaaaata tgttcggagg tagaaaacat gaagaccccg
     4921 ctaaccatcg aaatagaatc ctcgttatac caatattgca tcgagcaagg cggcttggtt
     4981 gtagaagaag tcacgatgcc tgatgaccaa ggaattgtag atacgcttag ctgcttgaca
     5041 aaatttgatg gaagccgaga atggcgatgc tatgagctga aagtgacaaa agcagatttt
     5101 cgctctacag ccaaactgtc atttgttgga cattataatt attttgtttt accaagtaaa
     5161 ctatatgaag aagtaaaaac tgaaatccct acaggaatag gtgttttagt gtatcgtccg
     5221 tacacggttg aagaggagat gcctgcctct ggtacatttc tgattgcgaa aaaagcagca
     5281 aagaaagagc ttgctgtacc ggaagaagct ttgacaaacc gcttcatggc ttctttattc
     5341 agggaagtaa gaaaagcaaa gcaaatggag cgaggcgtaa aatattttcc aaaagatcaa
     5401 ttgtacaaag aattgaaaag acgttcagaa aatcaagatc cattttctaa cgaacgatat
     5461 tatgaacggt ttttagagga aatagctagt caggcaatca atgagttgca agaagaagtt
     5521 atcgcgcttc agcatgatta tgaatatttg aggcaacaac agcagatccg tcggttgcct
     5581 acagagccat tggagtgaaa aatatgtact atccttattt aagaggaaaa caatttgacc
     5641 tattagcatt aaaagaagca ctgtcgcgag gattgctctc caataaaatc caaccagtga
     5701 tcgaaccagt acgtgattcc gctacgctta aaaatgtgat cgaattattt caaaaaaagc
     5761 accatccaat cgctgtgatc caaaatcccc aagtaggaca attcaaatta ttcgatcagc
     5821 atgtgcacag ttggacagtc aaagaaaata gctctgtcgt ccctgcacag atcttaacgc
     5881 cagaaaatct cagtgaagta ctggattcac ctccagcgtt tcttgttttc gatggacagc
     5941 actacccaaa agatacagag gtgtggaaac agttggcagg agttgattcg aaatttttga
     6001 taccagatac tagtcgcttt cgcatctggc tgcccgaaaa caaaattgtg atccgtgact
     6061 cttttcaaac gagaaagcat gttgaaagtt atgcagataa aacagatgac ttttttagtg
     6121 atgactatct ttttttccac gcagacggat atattggatt ttctgatttc acgatcgaag
     6181 gtagccgtta tttcgataaa gggttcccta gccgagcgct tgctatccat ctaacgtata
     6241 ttgatgctta cggcaatata cgcgtgaaac attttgtctc tgacagtaac gactctgcta
     6301 aagaccaagc attgaagttt cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc
     6361 accatcatca actcttgatt acttcaggaa tgattgaatt attggctctt taccagcaac
     6421 aaaaattccc tggactggga gtgttgaaaa agtggtcact tatgcatcat ttggaactta
     6481 ttagtcacct gctagatcat ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac
     6541 tatatgaagt gatccaatga ttagataatg taaatgaaaa aggaatagaa aagaggaaac
     6601 tggatgtata ttcggcaaac aaggaaagaa gaagtagaag aagtggtaga aatcattaat
     6661 gccgcagcgg atttcattgc ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca
     6721 acaaaagaaa cgattttgaa cgatattgaa agaaaagaaa gctatgtatt aatagtggaa
     6781 gatcaaattg ctggaacagc tgcattagtc tcaggcgtgg atcccgttta cacaaaaata
     6841 gatggtagtt ggaaagaatc aactggtcct tatttatcga ttcatcgtgt cgctttagca
     6901 gaaagtactc gaggaaaaaa actagcaaaa ccgttcttag aacatttgtt gacgattgca
     6961 gccagcaaaa atatccaaga tatccgtatc gatacttatc cagctaatga accgatggaa
     7021 aagaccatct attctgctgg attcacttat caagggatga ttgaatttcc ttttgaacac
     7081 ggcgaaagaa aagcatatca aataatcatc aattgactat aaaatcatct gagcaaagat
     7141 aaagacatta acactactga aaaaaacaat catatgatag ccaagaggtt gtaacagaag
     7201 tggccagctt caaaaaacaa gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat
     7261 tttagcgtat tttacgaaat agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca
     7321 tagcttttgt cacgatctct cggcttatcc tacgagtatt attcgataca tttatatcaa
     7381 taatcctttt tttattttat ctgcagcttc ttggaacgct ccatcgtttg acacgactaa
     7441 aagtgagggg atagctttca aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag
     7501 tctggctgaa atctgttcag atgaatctcc acgttttttc aggcgcgatt gcaatgtttc
     7561 tttagagatc gtaataaaga taggacggac taccgtcccg aaatactcat ataaatgcca
     7621 aaaaccattt gctgtcaaaa cggcgtaaca atttcctact gtcagtttct ttttaatttc
     7681 tgctttactg gaaccatatt tatgttgagc atattggtcc cactctgcaa actcatttcc
     7741 cttgatcatc tgattgaatg tattgtcaga aacaaaataa taatcctctt gatctttttc
     7801 cccaggtctt ggtgatcgat tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc
     7861 ttctgcgagt gaagtttttc cagaaccaga aggaccgata aatacatagc aataattcat
     7921 tgtcatttct cctttgatga ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct
     7981 tttaaaagta actttgcttt tttgtatttt ataccagcaa gccagtcaca atagcactta
     8041 aaaagctgac taaagtcgct gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc
     8101 ctttttcttc atttaatccc ttcatcgcac cagaaatgat cccgatagaa gagaagttgg
     8161 caaatgaaac gaggaagaca gatataattg ctgttgtacg ctcagtaaaa tggaaattac
     8221 catttgctaa ttctgtcata gcaacaaatt catttgtaac cagtttagtc gccatgatac
     8281 taccggcatc cactgcttct ttccatggga ttccgctgat gaaggcaaga ggggcaaaga
     8341 tgtaacctag gatctcttgg aaagtgatgc cgaatatccc gtggaagata gcattgatca
     8401 tggcaatcaa agcaacaaaa ccgataagca tagcagcaac cgtgatcgcc acatggaaac
     8461 catcaagaat gtattctccc aacatttgga aaaatgtctg ttctttgttt tcttcaatga
     8521 tcagctcatc ttctttttct tctaatacat agggatttac aattgaagcc agaatataac
     8581 cgccaaataa gttcaaaaca agggcagtga tcacatattt agaatcgatc agtgccatat
     8641 aagatccgac gatcgacata gaaactgtag acatagccga tacactcatg gtgaacaaac
     8701 gtttctcaga taggaagggt aattcctttt ttatggaaat aaaaacttcc gattgtccta
     8761 aaatagcaga agctactcca ttataagatt ccaatttgcc catcccgttg attttactta
     8821 atcctagacc aacagctttc ataaataatg gaagaatctt gatatagcgt agaataccga
     8881 taatagcaga aataaataca attggcatca atacggaaag aaagaaaggt gcacttcctt
     8941 tatttgctac accggcaaaa acaaagtcaa caccttgccc agcaaaagca agaagatgat
     9001 caaacatttt tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt aataatacga
     9061 aaccaagaac aagctgtaaa acgaacatca caattagtgg tttcaatttg attgcttttt
     9121 tgttactgct gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata atacctaata
     9181 aataactcaa ataatcactc ctattccaaa ttccgtttta ttttacctgt tttgcaaata
     9241 aaatactaat ctttagtgaa attttcatta cttttttaga gtgaaacgca ttcattttcc
     9301 attagaaaat aaagcgaaca aaataaaagg tttaaaaagt gtaa
//
LOCUS       DACCXM010000103         9194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCXM010000103 DACCXM010000000
VERSION     DACCXM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.106"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            63..1277
                     /locus_tag="IVY61_002201"
                     /pseudo
     CDS             63..1277
                     /locus_tag="IVY61_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1791)
                     /locus_tag="IVY61_002202"
     CDS             complement(1456..1791)
                     /locus_tag="IVY61_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7971279.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="IVY61_002203"
     CDS             complement(1775..2047)
                     /locus_tag="IVY61_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP7971280.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="IVY61_002204"
     CDS             complement(2498..3301)
                     /locus_tag="IVY61_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7971281.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="IVY61_002205"
     CDS             complement(3279..4403)
                     /locus_tag="IVY61_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7971282.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="IVY61_002206"
     CDS             complement(4419..5234)
                     /locus_tag="IVY61_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7971283.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6105)
                     /locus_tag="IVY61_002207"
     CDS             complement(5224..6105)
                     /locus_tag="IVY61_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7971284.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="IVY61_002208"
     CDS             complement(6181..7470)
                     /locus_tag="IVY61_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7971285.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="IVY61_002209"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="IVY61_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(8819..>9194)
                     /locus_tag="IVY61_002210"
     CDS             complement(8819..>9194)
                     /locus_tag="IVY61_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7971286.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2829 a         1966 c         1466 g         2933 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca gccagactgt agacaaaagc tgttccaaac
     8881 tcgtaactcg agcttggaac agctttttat ctacttcgtt tcgaattttt gaacaaaaaa
     8941 ataaaggctc cttgcctttc catagccagg tggctaattt ctttaaattc attgcagcaa
     9001 acactagcat cgtatgcgtc gcgacttttt ccaagcctcg atacttggtc caacgcatcc
     9061 catgcttttc ttttgcatcc gcaaatattc gttcaatcgt ttgtttccgt tttttgtaaa
     9121 ttgatttatt cagatctgtt aggcgaagat gttccacttc atccatcaga tctccccaaa
     9181 catgtctcgt gatg
//
LOCUS       DACCXM010000104         8957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCXM010000104 DACCXM010000000
VERSION     DACCXM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.107"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVY61_002211"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVY61_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP7971287.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVY61_002212"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVY61_002212"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP7971288.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IVY61_002213"
     CDS             complement(2169..2906)
                     /locus_tag="IVY61_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7971289.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IVY61_002214"
     CDS             complement(2997..3524)
                     /locus_tag="IVY61_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP7971290.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IVY61_002215"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IVY61_002215"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP7971291.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IVY61_002216"
     CDS             complement(4401..4886)
                     /locus_tag="IVY61_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP7971292.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IVY61_002217"
     CDS             complement(5039..5236)
                     /locus_tag="IVY61_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971293.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IVY61_002218"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IVY61_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP7971294.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IVY61_002219"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IVY61_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP7971295.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
BASE COUNT         2532 a         1986 c         1498 g         2941 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaa
//
LOCUS       DACCXM010000105         8831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCXM010000105 DACCXM010000000
VERSION     DACCXM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.108"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            8..307
                     /locus_tag="IVY61_002220"
     CDS             8..307
                     /locus_tag="IVY61_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971296.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(404..838)
                     /locus_tag="IVY61_002221"
     CDS             complement(404..838)
                     /locus_tag="IVY61_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7971297.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1070..1780)
                     /locus_tag="IVY61_002222"
     CDS             complement(1070..1780)
                     /locus_tag="IVY61_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7971298.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1770..2630)
                     /locus_tag="IVY61_002223"
     CDS             complement(1770..2630)
                     /locus_tag="IVY61_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP7971299.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2635..3273)
                     /locus_tag="IVY61_002224"
     CDS             complement(2635..3273)
                     /locus_tag="IVY61_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP7971300.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3288..3587)
                     /locus_tag="IVY61_002225"
     CDS             complement(3288..3587)
                     /locus_tag="IVY61_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7971301.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3630..5114)
                     /locus_tag="IVY61_002226"
     CDS             complement(3630..5114)
                     /locus_tag="IVY61_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7971302.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5134..5595)
                     /locus_tag="IVY61_002227"
     CDS             complement(5134..5595)
                     /locus_tag="IVY61_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7971303.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5613..6680)
                     /gene="ulaG"
                     /locus_tag="IVY61_002228"
     CDS             complement(5613..6680)
                     /gene="ulaG"
                     /locus_tag="IVY61_002228"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP7971304.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6898..7668
                     /locus_tag="IVY61_002229"
     CDS             6898..7668
                     /locus_tag="IVY61_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7971305.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7941..8768)
                     /locus_tag="IVY61_002230"
     CDS             complement(7941..8768)
                     /locus_tag="IVY61_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971306.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2678 a         1860 c         1384 g         2909 t
ORIGIN      
        1 ataaaacatg gattatactg ttgaatttga cagtgaacta cttaagcaat ttccacaaga
       61 agaacatttc atgtataaag aagcaatttc ccacaaagtg acagagtatt tggagcataa
      121 tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa ggaatatacg agatgaaagt
      181 caaaattaat aaaaaaaatt atcgcatagc atttgcttta aaaaaaaatc atgtgttcat
      241 tttttatatt agttcgaatt tgcaaaaaat tgtatttgat aaagaggtga gtaaaaaaat
      301 aaaatagctg agcattggag gatagattga gtgatactaa gtctatcctc ttgtttttct
      361 aaagaactaa gctagaattt tttcttttgt acgttattat atgttatttg tcattaccaa
      421 aaagcacctt ttttcttcta gtatctatga cataagaaat accacccatt tcctttactt
      481 gaacaaatcc tactgattca taaaattttt ttgttttctc agtatcatct gtcaataatt
      541 cgatttggac tggtccataa ctagtgatct tctccaaagc ttcattcaaa agcactttgc
      601 ctattccttg gcgctgatac ctcggatgaa tgaggatatc ttgaataaat gcgatcgtcg
      661 caaaatcagt gatccaacga acgattccga taatcttccc ttccccatta cgctttatca
      721 gagattcaga ttgttcaaat gctctagcta accttgcagt gtcttttgtg taattcgtcc
      781 aaccaacgct cttatataat tcagaaatct catcgatcat aagggaatca atattcattt
      841 ttttcttcct ttcttataca attctccctc taataataca gttctttgac tatttaatct
      901 atcacatata actctcctgt aaagattctc tcgattcctt aaaattcctc tttcctttta
      961 gaatgcatac tttttgataa ataaaaaggt tgtgataaat tttcttcaca acctctgttt
     1021 ctctctatca agtgttatct tttaattttc tacccttacc ctagcatctc tattgcccgt
     1081 agtatgcata ctttccatgt ttgcgaaaat aatgtttatc tagtaaatat tgcgggattt
     1141 cacatatatt ggggttgatc agctcgtttt ctttggccat cagacaaatt tcatctaaga
     1201 tcaaactgtt ttctactgct ttcataggtg aatcccccca tgtgaacggt ccatgtccat
     1261 agacaagtac tccagggact tcgtttggat ctaattttct ttccttaaac gtctcgacaa
     1321 tcacattccc tgtatgaact tcgtaagctt ctcttacttc ttctttcgtt aattggcgtg
     1381 tacaaggaac cttaccataa aaagcatcag catgagtcgt gccataagct ggcaaatcgc
     1441 gtcctgcttg cgcccacatg acggagtgcg tggaatgagt atgagtaata gcagttatat
     1501 cttcaaaagt ttgatacaaa acaacatgtg tggggagatc tgaagatggt tttaagctat
     1561 cttcttctat tatatttccc gacaaatctg ttaccaccat ttgatctgct tgcatgcatt
     1621 catatctgac accgcttggc ttaatgacga tgactcctag ctcacggtcc acttcactca
     1681 cattgcccca agtcaatttg actaatccgt atcgcggcaa tgccagattt gcttcaagga
     1741 cacgttgctt cattgctgca attttttctt cacaagacat catagccagc ctccttcaat
     1801 ttagaactta agtagtcttt tgcctgctgg atttctcttc gaaagtcttg gcttttttca
     1861 ctccacattt caatcagaaa tgttccatca taatctagac gtttcaaatg tcttaataat
     1921 ccaagaaaat caacacaacc gctaccaaaa ggaacatcac gaaactggcc tgaatattct
     1981 tttgttacag ggtaagtatc ttttaagtgg atagcagtga tataatcgat ccctttctca
     2041 agttctcgag ccggattatt ttccggccat gcggacaaat tgcctaggtc tggatatact
     2101 tgtagaaaag gtgaatgaat ctgttctttg atttctaaaa attttgaaat ggagttcata
     2161 aatggatcat ccatgatttc cacggaaaga atgatgccat atttagaagc ttcctttacg
     2221 ctttctttta ttccttcgat aaagtcttct ctggaattca ttgatttctc ttcataataa
     2281 acatcgtatc cagctatttg gattactttg acgcttagat tatgcgccaa atggattgct
     2341 tttttcatta gttgtttcgc tgcttttttc ttttctggat cagcagaacc aaacggaaat
     2401 cgccgatgcg cacttaaaca aatcgaatgg atacgtacac cggagctgaa catctcttct
     2461 ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg ctaaccgctc atccgtctca
     2521 tcaatcgaca tctcgataaa atcgaagtcc atttctttta caagggaaaa acgttctttc
     2581 catgaaatat ctttgggtaa cgctttttca taaatcccaa tttgagccat ttccttaccc
     2641 ccagatacga atgatctcat ctttgaagtt ttgtgcggcc ttcatcggat catttgctgc
     2701 tgtaatacca cgaccagtga taaaggtata cacatcgatc ccttcaaata agtgtaatgt
     2761 gtgtggattt aatccaccag ttacagaaac attaaagcct agttcaatca gttttttgat
     2821 cttcgataaa tctttttcac cccaagtttc accagataac agcgcatcac ggctttgatg
     2881 gtagattacc tgccggatac cgatgttatg ccaatccatg gcttgttcaa aagtccaatt
     2941 gccgtagagt tctacttgca attcacctat ttctttttgt gctgcctcca tagttggaat
     3001 cgttgcacaa caaatgactg tcatccagtc tgctcctgct tttgcaacat tactcgccac
     3061 tgttcctcca gcgtctgcac atttcgtatc agcgactagt tttttatctg ggaacatact
     3121 gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa agaattgtgc ccacttcaat
     3181 aatatctact acatcaccaa ctttcataac atctcctaat gcatgttcta agtcattgtg
     3241 atctaacgca atttgcaaat tcggcctgct catcttagtt ccttccttta cttgtttttt
     3301 tggtaccact ttgtttcagc aaatttttca ctgatttctt tgactgacat cacatttttg
     3361 ataccgataa ctgtaactcc ttttttctct gcctgagtaa acatattcaa aaaattgact
     3421 ggcgtaaaaa ctacatcgta ttggccagct gaactttttc cctctgaaat ggcacaatga
     3481 tggattttgg taattggtac gtttagttct ttcattgctt tttccacact tctcatcatc
     3541 atcaagcttg ttcctgatcc atttgcacac gatactaaaa ttctcatctt aatccctcca
     3601 ttatgagtgt tgtctaatca gattttcttt tatactctct gcttttctgc atatgtgtca
     3661 tagtcttcta caatcaaaaa ataaccatca ggatttttcc gatattgtaa ctgcggaata
     3721 gccaataaac caatgacaac tacggcaatt ccagcataac ctaggacttt catgattact
     3781 gtaaacaatg gccaagctgt tgcccagtcg aacatcccga tataaccacc gtatgttgac
     3841 atcccaatcc atgtagcgaa taatgctgaa ccgcctactt gtatgattcc tgaaagaaac
     3901 gggaacaggc aagcggcttt gaatccacct cggttattgg cataaactgc tataacggca
     3961 ttatcaaaga acaacggaat gaaaccggct atgacgatcg ttggtgattt caataaaatc
     4021 agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca agaacccaat cgttaccgca
     4081 tttggcgaac caaagccaaa agtagcagct acgtcaattc ctggaacagc tcctggtaaa
     4141 atcgtgtttg agattccttg gaaagattga gttagttcat cgacgaatgt ccgaacacct
     4201 aattgcaaga tcgccagata taccgcaaaa tttaaagaag tcgtcataat gtagaataag
     4261 aaactctgcc cttcttgcat gaattgcgct tgaatcaaat aatctttgcc tagtacaatc
     4321 agaatgatcc caaagaaaaa tagcatgagt aacgaagtag caaccatatt ctcattgaaa
     4381 atcgacaaga atcctggtaa tttgatatct tcaatttttt tgtcttgctt gattgatcta
     4441 tttttctttt catcccgctt tttcatccac tcagctaatt ttgcaaatat aaagattccg
     4501 aacatttgct gatgtgccac tgcaaatccg gcaccttctg tcaattcctg cgtgattcct
     4561 actgttaagt tagaagctac tgtccaataa caacctaaaa tcagtcccat aaaaatcaat
     4621 gcttgtatcc tacctagatc aggaaagcaa aacagtaaaa tccaaaaagc ggtagctgct
     4681 tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca gtttcgtata tttctgaaaa
     4741 cggactaata agatatttaa aacaaatgca atcagtaata aaatcatcac atctccaaag
     4801 gtacggccaa atgtctccat taatccagca tccacagcat tttgtccaaa ataaggatct
     4861 gtaaccatgg catccaaatt gaaccgttcc tttagtccaa ccaagattgg ccgaaaatta
     4921 ttgaccaaac caccagagcc aacagtcaaa ataaagtaac cgaccgttgc ctttaagaat
     4981 ccagcgatgc attcatgaag cgggcgtttc agcaaaatat agcctaataa cacgataaaa
     5041 ccaatcaggt atgctggctg cgtcaagata tttgttgcaa aatacgtcca aatgcttaac
     5101 aaaaattccc ccatcttttt tctcctctat caattaatta tattctgcca tcacaacttg
     5161 gtaatcatcg atggagttcg tagagatgag acgctcaacc aatccatctg tcattaataa
     5221 ttcggataat ttttggatat tttctagatg ttcttctgga tcttttgctg cgagtgtaaa
     5281 aaacaaacta gccgttttct cctcattctc cccttcactt tcaaaacgga ttggctgttt
     5341 caacttggta aatgaaatag ctgttccaaa aacaccatca cttttttctg aagaatgcgg
     5401 cattgctact tgaggtgcga tcacaatata aggaccgtat ttttctacac aatggatgat
     5461 ctcctccaca tacgtttcat caataatttc cttatcgatc aatacaagac agctttcggc
     5521 gatagcttca cgccaatccg tgatttcttt atctgggtaa tgaatcaatt gatggtcaaa
     5581 aaagtatttc agcaagaaac tccccttctt tcttataaaa acgacggaaa cggtgtatca
     5641 ttttcaatcg agaagacatc ttcaaatcct cgataaaaat taaactcaag atcatctttg
     5701 tttgtcggat agatgaattt cccgcctact tgccagatat aaggtttaaa tccatacttc
     5761 aggcgatcct ttttgaattt ccaaatttca gtgatctctt ttggatcagc cataaaatta
     5821 gaccaaatat cgtagtggac aggaatcact acttttgcat tcaatgactc cgccatgcgc
     5881 agcatatcaa cagaagtaac tttgtctgtt atccctcgtg ggttttcacc ataggcccct
     5941 aaacagacat cgattttgtg ttcattacca tgtttggcaa ataaattgga gtaatgtgaa
     6001 tctcctgcat gataaagatt ccctcctgat gtttcgaata aatagtttac tgcaatttga
     6061 tccatctcct gaggcatttt tccttttaat acctcttctg gatcttcaca agtgaccaac
     6121 gctgtccgat caaacgcttc taaagctact acattgatgt ctttgatttt gactgcatca
     6181 cctggcttta ctgtaatcgt tttttcttca ggaattcccc atttttgcca cgtgcgaacc
     6241 acttcttttg gcccaatgaa tgttgcttca ggacaatttt gatgaactgc tgcggctgta
     6301 tggatatcta gatgatcgga atggatatgt gtaacgatta aagcgtctac attttttatc
     6361 gcaaaaggat cgattacgaa aggctgagta cgtaaattag gctgcatatt ttgaacgcca
     6421 ctcatacgca tcatctgatg gccttttttc atcatgccat tcccatgact acgttttccc
     6481 gtcccacacc atagatcaca taaaatattc gttccttcat gacttttcag ccaaattccc
     6541 gtacagccca gccaccacat agcaaaagtt ccaggatcca ctacttctgt ttcgatttct
     6601 tcattcaagt atgtgcccca ctcaggaaat gtttctacta accacttttc ttttgttact
     6661 tcattgacag ttttactcat ttgcttgctc cccccatatc gttgttccaa cttacaagta
     6721 aatattaatg tgtatcagca tccatgaaaa cccttttata tgaccgtttg ttttttatat
     6781 gattttcttt cagtaatcaa tgattgtttc agtataataa agacagatat gaatgtttaa
     6841 atgaacaact agtcaaataa atccatggat caagatgtcc ctcaggaggc aaaagtcatg
     6901 aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa ttttggaact gcttcagcag
     6961 actgaacaac tatcaacttt cgaattagct gaatcccttg atgtatctat tagtacgata
     7021 cgaagagatt taaatttatt agaagaaaaa aacgagatca tacgcaatta cggttattgt
     7081 tcttataatt acaaaaacca gactgatttt gatcaatctg gtcctgaaag gattaaacaa
     7141 gcgattgcca gagaagctag taaatacatt aacgattatg atacgctgtt tatcaattct
     7201 agctcgactg cgttgaaaac actggatttt cttcaatcta accatttgac agtcattaca
     7261 aacaacgtga aaatcaatta ctctccgcac aaagataatt gtagttatat tttaactggt
     7321 ggagagatgc gctttccaaa agaagcacta gtgggcgaca tcgcattaaa tacgatttct
     7381 tcagtcaatg cagacatttg tatcattggc tgtagcggag tcgatgtagt acatggggtc
     7441 acaacaaaga tattaaatga atcaacgatc aacgaaatga tggtcaacaa gacaatcaaa
     7501 tgtaagattt tagtcgctga tcatcgaaaa atcggactga catcaaagtt caagattgct
     7561 gatatttcag tctttgatta tttgatcact gataaatttt gttctgaaaa agtattagag
     7621 gagatcagaa atacggggat aaaaaccatt caagtagatt cattttaaga accaggacgt
     7681 aaatatgtaa ataggattat caaaaaaatg aataacaaat ttatcgataa atgaaaaaca
     7741 cgctacgctc aaattgataa aggaatccca agatgtatcc atccaatcga taccatcata
     7801 atctattgct tcttttataa ccgttatatg ttgcctaaat aggaatcaaa caatatcgaa
     7861 aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa taaacggtat tcaaatcaga
     7921 acactgattt cacaaccact ttatttcagg tcgttttcaa tcacctttac cgcgtaatca
     7981 tttccgttga aacgcgctat tttttttgcg tccatatatc tttctaaata atcatcttct
     8041 cctaattgct tatggcaaga agcaatttca taaagcaagt attccaatgc ataagtcgat
     8101 ttatgtttct tattatactc aatacccttc tcacataatt ggattgcttt atcataatcc
     8161 tcgattttac ggtaataggt ggcgatatca taatatacaa tgcagaccat cagattatca
     8221 aaatcagtct cactcaatat tttaagtgta cgctccacat aaactttcgc aaaatctaat
     8281 tctccttttg acaagtagat cattgccacg tttgcagctg cttgtaagtg atggatatct
     8341 gtttcggtcg tttgttccaa cacttgatta aaataaaata atgctgcatc tggttttttc
     8401 gcataaagat gatcaagcag tccttgataa aataaaattt gatttaaaag atcagaatta
     8461 cttacttttt cctgatcgat cgtttgtaaa attttataag attcttggta ttttgataca
     8521 gaattcaatg attgaatctc atttattttt ttcctgattt tgtcttcttc tgtttctact
     8581 aatatatcgg agatctcgac attcagcttt ttgcaaagcg cacttaagat attgatgcct
     8641 ggcgacatat tcttgttttc gatattgctg acagttgctt gttcacaaat gccttctgct
     8701 aattgtactt gtgttaaccc ctttttcttt cggtattctt ttagtaatgc tccatctact
     8761 ttaatcaaaa caatttcctt ccctttaaaa aattatttct cctttatatc atacacaaaa
     8821 aaaagttcta a
//
LOCUS       DACCXM010000106         8742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCXM010000106 DACCXM010000000
VERSION     DACCXM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.109"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(357..1226)
                     /locus_tag="IVY61_002231"
     CDS             complement(357..1226)
                     /locus_tag="IVY61_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7971307.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1246..2367)
                     /locus_tag="IVY61_002232"
     CDS             complement(1246..2367)
                     /locus_tag="IVY61_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7971308.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2369..2764)
                     /locus_tag="IVY61_002233"
     CDS             complement(2369..2764)
                     /locus_tag="IVY61_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7971309.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(2783..3580)
                     /locus_tag="IVY61_002234"
     CDS             complement(2783..3580)
                     /locus_tag="IVY61_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7971310.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(3582..4370)
                     /locus_tag="IVY61_002235"
     CDS             complement(3582..4370)
                     /locus_tag="IVY61_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7971311.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4384..4860)
                     /locus_tag="IVY61_002236"
     CDS             complement(4384..4860)
                     /locus_tag="IVY61_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7971312.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(4932..7589)
                     /locus_tag="IVY61_002237"
     CDS             complement(4932..7589)
                     /locus_tag="IVY61_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7971313.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(7608..8012)
                     /locus_tag="IVY61_002238"
     CDS             complement(7608..8012)
                     /locus_tag="IVY61_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP7971314.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(8244..8501)
                     /locus_tag="IVY61_002239"
     CDS             complement(8244..8501)
                     /locus_tag="IVY61_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP7971315.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT         2754 a         1782 c         1236 g         2970 t
ORIGIN      
        1 cacttaaaaa agcactaaaa ttataagaga gggaggtaat ggtctgaaca ccaagaattg
       61 ttccaacatc gaattgctta actaatgagt tcaaagctta attatgtggc tcttatattt
      121 accgagcgtg gattgcgatc tctttagcga aacaaacatc tttcataaaa aaaacagcca
      181 tatccgcttt cttggatatg actatttact actactttac tatctttcat tcttcttgtc
      241 tcacttatga taaggcgctg ggtatcatga gtgaatgata ttgctgaata caaatagtcc
      301 gaccacatta agttgatcgg acttcataag cattttattg actaatactt ttttgcttaa
      361 gaattgattc taacatcttt tgctaaagtt aagcatccta tagttcctgc attatctgct
      421 aacttaggtg tgacaatgta ttcttcgata tttggcgtat cgacatagcc gttaattagt
      481 tctaaaaaac gtctttgtac tttctttttc agatgttctt gttgcatgac accaccacca
      541 aaaattataa tatctggtga aagcatcagg gtcgtattac aggcacattg tgcaatataa
      601 tccgcctcga tttcccagaa cggatcttct atagaaagat cttgtccctt ggttcccgta
      661 cgcatctcga tagcgggtcc agatgccaac ccttcgagac aatcatgatg aaaaggacaa
      721 gtaccagaga aagtatctcc tgaatgtctc ttaactaacg tatgccccat ttctggatgg
      781 ctaaaccctt caacaaaaat cccatcttgt atagctcctc caccaattcc agttccaaca
      841 gtgtaataaa caacactgga taaacctttt ccatgacctg aaacatactc cccgtaacaa
      901 gcagcattca catcggttgt ccaggcaatt ggaatttcga aatgcttatt caacgttcca
      961 acaaaatcaa aatttttcca ggcagttttt ggagtagagg tgatatgtcc ataggtcttt
     1021 gattcacgat ggatatcaat cggtccaaat gaccctactc caatgctttc taattgttct
     1081 ttatagtgat taaagaaatc aattaccaaa ctcattgttt cattcggtgt agttgtagga
     1141 aaactcactc tttctaaaac ctttaaatca tcattgccaa tggcacaaac aaatttagtc
     1201 ccacctgctt ctattgagcc atacatttct tactccaccg tccttttatc taggaattcg
     1261 ttcttccctg tctaatagtt tttcgaaaaa ctcatgtggt tccttttggt accaataggc
     1321 aacagaagaa atatcgtctt gacgctcata aagcttgatg tcatcatttc ctagggtttg
     1381 gaaagtaaca cgtaaatttt ctttgaaact aatggggtcg ggtaaatgcc agcggtacaa
     1441 cccatgcatc ggtaaagcat catcgccaaa accatgaact ggatttggat taggttttcc
     1501 agcactaaaa taatcccggg tgtgatctct tttagattga aaaggatagc ccataaatag
     1561 cgtattaaaa ttttgagcat atgccctttc tccaaagttc tttttatgga aagcccaagc
     1621 gccgccaaaa taatcttctg ctcctgtaga tgttattgta gggaaatctt catctccatc
     1681 tatataaaac ttaaattctc cttcccccca ccagtatctt tccaatgcgc acaaagcaaa
     1741 gtaggttcct acataatacc cagtgccttt gattccatca ataagggtgt aatcttttcc
     1801 ttttgtcgtg atgggttcac ggttccattt agcatgaaaa tacatcgcat catccggtac
     1861 ttcagtcaat gcataattaa atgtataaaa aatgtgctta ataaactcag gatgttcatt
     1921 cgttaaagta atcttagctt tttttcgaaa tggcatctca aaatagcaat tcatccctga
     1981 tgttggattc actacgattg gtaaggaatt cacttcgcat cttgtcccaa acccattaca
     2041 aaaaaaatct cctaatggac tttctacaga aggagtggat tcatcatccc aataaatacg
     2101 caaaattaaa tcacgtaaga cgaaactccc agcgattgtt ttgtctgtca ctgtcatcca
     2161 aaaatgacgt aaaattccag ggccttccat ttctgctaaa gtaattgtct ctccagcatt
     2221 tatcggaaca gaaggtgacc cttttctccc aattcctaga tgactcgctg tcattgccgc
     2281 tttccctttt tcaccatttt tattttctgg tgaaatagtt ctactttcct ctcccttgtg
     2341 gagccatata ttatttaaac aattcatttt attcgtcctc tatcctttct tcactttttt
     2401 gattattcat atagatgatt tgttgttgtg cctccgcaat aattgctcca atatctaaat
     2461 tatcatttcc taactgcaaa gcgattgaca ttgccaaggg aagattcatt cctgcaatta
     2521 cgtgcactct gtcagaaata taggtaaaaa acttttgatt tacacttcct ccagccatgt
     2581 cagttaaaac aattatttcg tctgtagtat ccactaactc tttaatttgt tcatctatat
     2641 catcaatttc ttcgacatat gcacataagc tactaatttg attatgcata cctgttaaaa
     2701 actctagtgt ctctttaaaa ccctcagcta gtattgaatg gctagcaatg atcaatcttc
     2761 tcataattca aatccttttc ctttacgcta atattccaaa tgcagccata accatcgcaa
     2821 aaacaataac gagtagaatc aaaatggtca tactgatttt tttcttactg agtagataat
     2881 atgctgcagc agtaataatt actggaatta atgttggtaa aattttgtcc aacattggtt
     2941 ggattttcat actaacttca ccaaaagtga atttaagtgg cgttgaaaca ctgataactg
     3001 aagcaattaa tgatccaact actgttagcc ccaatactga agcagcttca gttaaataat
     3061 taatcttttc accgtattca gtaacaatct tcattccctg cttataacca aattcaaagc
     3121 ctctcattcg gaaaaagaag attaccatga ttaaaatgaa ccataaaaag attcctacag
     3181 gattattttc taaagccata taagccgcaa ttgatcccat gatggttggg atcatcgtca
     3241 tcaaaagaga gtccccaata ccagcaattg gacccatcgt accaacttta aagtcattaa
     3301 tcgcatcttt tgcttctaat ccttgctttt cctccattgc aacacaagca cctaaaataa
     3361 tggctgccag ccaaggctgt gtattgtaat actgaaagtg attatttaaa gcttcgatgt
     3421 attcatcatc atctttgtat aattttctta aaataggtga caatgcgtaa gtcacagatg
     3481 caccttgctg tgtttgataa ttaaaggtac aaactgacat caaccaacga tagttcgctt
     3541 tcctaagatc ctttttcgtt acttgatatt tttcgctcat tttattcatc ctccccaata
     3601 aacccaacat cagaagctcc cgcagaagca gtgatttgtg catctttttc agctctatga
     3661 tacacattaa atgctaaagc gaatcctatg atcgccgctc caataatagg taccttcaaa
     3721 aaagcggcta agaaaaatcc aacaagtaaa taactaatgt atcgacctgt aggcataaag
     3781 cgtaacaaca tcgcaatacc aacagcaggc aacatttttc cggctaatgt tagcccatcc
     3841 ataaaccaat taggtaaaac atccaaaacg taattaacag cagactgtcc taaagtaacg
     3901 gcaataaata ctggaattgc tgcagataaa ccaaagaaaa ttggccccaa ccacaaaaca
     3961 ctattcattt tcttaaattc tcgtttattc gcatattcct gtgatttgcg aacaacaaaa
     4021 ccatttaaga ttttgacaat tacatctagt tgcaccccta acatacctac aggtagtcct
     4081 attgctaatc cggcttctac tccaagctgg ctaccaaatg ccgttgcaat aattgcgcac
     4141 gttccatagt ccggtacaga tgagccacca aaaccggcaa ctcctaatga catcaattga
     4201 actgtaccgc caactatcaa acctgttttt atatcaccca tcaaaattcc tgtgaataat
     4261 ccccaaaaaa ctgcgtaatt tacaaagatc atcggaatcc cataataatc aacatgttta
     4321 ataaatgcta atatagtcag cataataatt tgaaaaatag taaaatgcat tatccttact
     4381 cctttatcct aattttgatt gaatttcctg tgccttatcc gccggaacaa attgtgtaac
     4441 aatttctaca ccagaatcca acaatttttt atacgtgctg atattttcat ctgatgcata
     4501 ggctctagaa gatagctgta atgcattatc tttaacaaca gtaccgccaa caattagtcg
     4561 gtcaattcta ataccgttat ctactaaatg aaggattgtt tccggctctt tcacgatgac
     4621 aaatactttt tcctgatcgt atttcccctt tttaaaattc gcaatcgctg tttcttcagt
     4681 gataattgat acagccattc cagcaggtct agctaaacgc atacttgatt tttggatgtc
     4741 gttatttgcg acagaatcat cgatcaccat cactctgttt gctttagaca ttggtgccca
     4801 ttgcgtgaca ataatcccat gcaataaacg gttatcaact ctcgacataa caatactcat
     4861 aaatacttcc tcctttatct ttcattttct tacaagcaat taccatgcca aaacaaaaag
     4921 aacttgataa atcaagctct ttttacataa tcataaacgt atttaacctc atgaatcggg
     4981 atgtcgacac tataataatg ttcaacgaca ctgaaagatt gcttaaaatg tgtctgaaat
     5041 ttttgaaatt cctcactcgt ttcattgaaa taaatttcat cttccaaagt attttgtttt
     5101 actaatcgtt caattaagca actaatgtga acatataatc cgaaacacgt attattatca
     5161 aatgaaattc ctagatcatt ttgtaagatg tatatagcat ctgaaacccg ttctaataat
     5221 ttagtcggat ttaatatggt caattgctcc attaaattcg ttagactaaa attcttcaga
     5281 atattattgt tcaaaacttt cagttcatta tcattaaagt gtctattcaa ttgattggag
     5341 aatttactta tattcattcc taaaatcata tcttcaattg aaaagaattc tacatcttct
     5401 agatgtggat ttagtgttcc aacaataaaa agaatattgt tctctttacg aagctccgat
     5461 aaatagtttt cattcactaa agagtaatag tcacacgttt ctatgacaag atcagtatcc
     5521 cttggaaagc tatcttcaag tatccttttt aatttaaacg aagttcctaa tccgcttgca
     5581 caagaacaga caatcatatc cttcttaata tttgatgatt cataaatata atccacattt
     5641 tgattttctt ttacttgttc aagaatctgt ttaagcggta aatctaataa tatctgattt
     5701 gcaacatcaa tagccattct tgttgaaaca ttgtttagta gtccgaattt tttttgagcg
     5761 tctagttcca aatttagata aattttctcc agtgaaccca tatcaacaag taaaataatt
     5821 tcttcatatc cggtacactc ttgtaagtat ctattaattt tgtctgcaat ctcactactt
     5881 gaagtcatta gtggcatatc aatggcgtcg tatatatatc tctcaacgat cctattagca
     5941 gtacttgcta tacaggttgc cgttagatct ccatgagcca aaatcagtgc taaacgtttt
     6001 tgctgaaatt ctttactaca cattaactta aataaaataa ccaaaacatt tttttgaatt
     6061 tttgttaaat aaatatcccc tatttgaata aaacgattga ctaattgaac ttctcgacta
     6121 caccaactat taatatcatt gatgaaggaa tataatttat cgtccgatgt taattggatt
     6181 tgataaatcc aagaattcaa aaagatagac gttatttgaa gataataata gtcaaattct
     6241 atttgaaaat ctctctctat ttccttaaca tactgtctaa atcgaatctc ttgataggtg
     6301 ggatatgtta aagctgaatt attttggata tcaacattct tttgtctaaa atatctatca
     6361 tatttctcaa aaagctgctg cttatcagtg tctttcttcc acgtttgata aagatactct
     6421 ataagttcgt tgatattaaa aacttcgtct actttttcca aatcatccat ttgaatatat
     6481 tttaattcat tctgtctttt cataaccttc atgccgttga aattcaccca ttgtggtaaa
     6541 tgatacatct gaatataaag ttcttccaaa tcatctaaga aataaaaggc attggcacag
     6601 gtcaattgaa tgatattttt caaattgcca acattttcac taaagcgata gtttaataaa
     6661 aaatctaata attgttgaga tatcagtatt ttctttgcta gtctttcttc ttctgattta
     6721 aaaaatatct tgattaactg ttttctttca taaagagaac gactgtctaa tgttggaagt
     6781 gttagcttta caggtattct tctcaacaaa gttgataata gtttatcctc aggtggttct
     6841 gatgttgcaa atataatcca gcaacttgat gtgtaccagt tttcattatc acccatccga
     6901 tgaaatttcc tcgtatccat aaactgaaaa agtttctcct gacattcatt gctcaatgca
     6961 tgaatttcat ctaaaaataa gactccatta ttagctaatt ctgctaaacc ctttcttcca
     7021 tcttcggctc ctgtatatgc accttttaca tgcccaaaaa aattggacaa gaaaagttct
     7081 ggattattgg catattcact acaattaact gaaatgaaag atgccttctc ttctatataa
     7141 ttattggcca acaaatcctt atacacataa tttgctaaat agctttttcc agttccagtc
     7201 tcgccatgaa gtaaaatggg taagccattt ggcggatagt agattgctgc tttaatttga
     7261 gcaattacat ttcgcagact tccttgactt ccgataaccc tttctattcc aaccctaaaa
     7321 gagctatcat accatttttc cgcttcctct gaagagctaa aaatttgttg atttggaact
     7381 ttctccggta aagaaaagaa gattactgga cgagtattga tttttccaaa ttctccatct
     7441 tttacgaatt cattcaaata ttgagagaca atattacgac taacatttaa tttttcagat
     7501 attttctgtg ctgtattgtt cccccattta tttttaatct cagagttctc tatgacataa
     7561 ttgacaatgc gttcttttaa ttcttccatc acagcacctc ctcgacacta ttcgaatttt
     7621 gccgacagta tttttccata aatatgactt gctaaaaagg ctttgtttag atcattcaat
     7681 tggaacgttg cttgttttgc ttgatccgct aagacgttga aaagatactc tggcgaaatc
     7741 gcatacccca aaatatcaac gattgttgca atcagatctt cttttgattc ttcatctgaa
     7801 agtaattcac gatacaacat cgtttgtttg cttactgtgt catattcctc taaggattca
     7861 tctgctaaaa gaaccacttg ttccaacaaa cggtcggata aatatttata gaaaatcagc
     7921 cccaataaat aatttttata ttctgacgca tccattttac tacggagatt atctgccgca
     7981 ctaaataact tctgatttaa ttctgcaccc aaaaagatac actcctatct cttatttaaa
     8041 gttgctatat accctcttat tttagcatac tcagttttag aaaaagcaaa aaaataaaga
     8101 aacttattct tgataacaag ctacgtgacg tatatcccta acacaaaaat agccatatcc
     8161 tttggtttgg atacggctat ttgatattac tcattcaatt atcaaatcac agtaggtgtg
     8221 aatctcatcg ggatcggcaa tatctacttc tgattaggat caaaggtgat tttctcaata
     8281 aaggtgtgat acaaagcttt aacttgcttc ttctcactct ggataagaaa gcaatccaaa
     8341 ctagtcaata tctgttggac atctttgact tgaatcggtt ccccttgttg ttgaaagatc
     8401 cctaagattt cattctctcg aatttgtaac tgtcttcgtt tttcagtcaa ttcatttaaa
     8461 cgatctttta acatcggaaa caattcggga ctatcttcca aagcagcttc ctgtttttgc
     8521 tctaatagtt agattttgcg taccattttc ttcttttatt ttccttcgat tgctgatttt
     8581 aatatagtct aaaggaaatt tatatttatt gtattcttta taacttcaag agaacttcgt
     8641 ttgagtatta ctgtattttt ttaattccat aagaaaatta gttattacct gccacctaat
     8701 cagcaaatct gaacctgagt caatataatt aatattattt tc
//
LOCUS       DACCXM010000107         8708 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCXM010000107 DACCXM010000000
VERSION     DACCXM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.110"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            38..1099
                     /locus_tag="IVY61_002240"
     CDS             38..1099
                     /locus_tag="IVY61_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7971316.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1102..1998
                     /gene="murQ"
                     /locus_tag="IVY61_002241"
     CDS             1102..1998
                     /gene="murQ"
                     /locus_tag="IVY61_002241"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7971317.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2012..3994
                     /locus_tag="IVY61_002242"
     CDS             2012..3994
                     /locus_tag="IVY61_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7971318.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4059..4505)
                     /gene="nrdI"
                     /locus_tag="IVY61_002243"
     CDS             complement(4059..4505)
                     /gene="nrdI"
                     /locus_tag="IVY61_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP7971319.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4716..6140
                     /locus_tag="IVY61_002244"
     CDS             4716..6140
                     /locus_tag="IVY61_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7971320.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6321..7094)
                     /locus_tag="IVY61_002245"
     CDS             complement(6321..7094)
                     /locus_tag="IVY61_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7971321.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7091..7996)
                     /locus_tag="IVY61_002246"
     CDS             complement(7091..7996)
                     /locus_tag="IVY61_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7971322.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8150..8470)
                     /locus_tag="IVY61_002247"
     CDS             complement(8150..8470)
                     /locus_tag="IVY61_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP7971323.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2728 a         1571 c         1865 g         2544 t
ORIGIN      
        1 atagaaatga agacggatac agaaaaagag ggataatatg tttggctttt caatatttat
       61 gaacgattct ttgtcagaag agaagaaaca gtatatccgc caaatggcat ccaatggctt
      121 tgtcggtatt ttcacttcca tgcatatacc agaagatgat gtttcggcat atcaaaagcg
      181 attgatcgaa ctaggaagcg aagcgaaaaa gaataaacta gaattgatgg tcgatatttc
      241 aggagaggca ttggaacgag cgggtttttc attagaagat ctaacgcctt tgaaagcaat
      301 tggtgtaaca ggtttaagaa tggattatca tatttccaat cagcagattg ccgactggtc
      361 acatcaattg aaaatcagtt tgaatgctag cacgatcacg cctaaagata tcgatgaatt
      421 gaaagaagca gaagctgatt tctcacagat tgaagcttgg cataattatt atccacgtcc
      481 cgaaacaggg ctggacaaag aatggtatca gaaaaaaaat caatggctca aaattcaagg
      541 tctccttgtc caaggttttg taccaggaga tacagagttg agaggcccac tatatcaagg
      601 attgccaact ttggaagaac atcgagggat ccatcctctt gcaggcgctc tagatctatt
      661 agcaagtaac acggatattg tttacatcgg agatgcaggt ctgtcagaaa acgttcagga
      721 gcaatttgct tctttccaaa aagaacagac agtcttgctc catacggaac ctgtagatga
      781 agaattttac gaatatatat tagggaagca tacgaatcgg caagacgatg caagagatgt
      841 catacgaagc gtagatgcac gttttcgaga gattccgcct atcccagcaa gaaatacagc
      901 tacgcgtatg aaagggtcta ttactttaga taacgaaaag tatttgcgct atatgggtga
      961 gatccaatta accaaatatg atttgccagc ggatgaaaaa gtgaatgtag tagctaaagt
     1021 tatcaaagaa gagctaccat tgatcaacca aatcaaagct ggtatgaact atcaatttat
     1081 aagaaaagaa ggaagataaa aatgagcaaa atcaatttag agaacctaac aacggaacgc
     1141 agaaaccaag aaacttttgg attagatgag atgagtattt ctcaagccgt tcaactaatg
     1201 aacaaagaag atcagaaagt ggccgctgcg gttgaaaaag aactgacaag tatcgaacca
     1261 gtgattgcta acacgattga gtcctttaaa aaaggtggac gtctgattta tatgggagcc
     1321 ggaacaagtg gaagactagg tgtgttagat gctgctgaat gcgtaccgac attcggtgta
     1381 gaaccggaaa tggtcatagg tctaattgct ggtggagaag aagcaatgac tgtagctgtc
     1441 gaaggggccg aagattctta tgagctaggg caagaagatc taatcaagct gaatttgaca
     1501 aaaaatgata tagttattgg tatcgcagct agcggtcgta caccatatgt gatcggtgga
     1561 ttagattacg caaaatcgat cggtgctgtc acaggaacga tttcttgcaa taaacaagcc
     1621 gaaatcagta aacacgcgga tttcccaatc gaagtcgatg caggtccaga atttttgaca
     1681 ggctctactc gattgaagtc aggaacagca caaaaactta tcttgaatat gatttctact
     1741 atcagtatga ttggtatagg taaagtattc aataacttga tggtcgatgt caaaccaaca
     1801 aatgaaaaac taatcgaacg ctcaaaacga atcatcatgc aagcgacaga agctgattac
     1861 gaaacagctg aaaagtactt tgaagaagca gatcaaaatg ttaagctggc tatcgtcatg
     1921 attttgacaa atagtgataa agaggaagct caagcaaaat tagctgaagc aaacggattt
     1981 gtaaaaggga ccttatgagg gggaaaagac catggcaaaa aacgaactga ctttggaaga
     2041 actggcaaaa caaatttata aaggggcagg cggcatgggc aatgtagaaa gcattgtcca
     2101 ctgcatgact cgtgtgcgta tgaagatcat caatgatgat cttgtaaatg tctctgaatt
     2161 aaaagcgatt ccaggagtgc ttggtgtagt agaagatgaa cagcttcaag tcatcatcgg
     2221 acctgggaaa gtgaacaaag tagcacagca aatggttgat ttggcaggcg tgaaattagg
     2281 agaaaaactt tcttctgtaa gtacagctac agcaaattca tcagcatctg gaaaagaccg
     2341 tgtaaacgaa cgggcacaag aaatgaaagc ggcacaaaaa tcgaaacaaa aaccatctaa
     2401 aatcaaatcg gtattaaaag acatttctaa tatctttgtg ccaatgattc ctgcatttgt
     2461 cggtactggg atcgtggcag ggattgccgc agttttagca aacttagtca cagccggaac
     2521 acttgatgca gcaacatggc aacaatatat cgatgtgatg aacatcttga aaaacgggat
     2581 gtttagctac ctagttatct atacaggtat caatgctgca caagtttttg gtgcaacacc
     2641 aactcttggt ggagttatcg gggccgttgt gatgctgaca gggatgaatc cggaagcacc
     2701 actgaaaaac ttatttactg gtgaagctct tgcagctgga caaggtggga tcctaggtgt
     2761 catcttcgct gtttggctat tgtcaatcgt tgagaaaaaa ctgcataaag tagtacctga
     2821 tgcggtagat atcatcgtta caccaactct ttcattgatt gccatcggtc tgattgaaat
     2881 tttcttcatc atgcctttag caggtttcat ttctgatggc atggtcggtg ggatcaactg
     2941 ggtacttggc gttggtggag catttgccgg cttcgttcta ggcacattat tcttgccgat
     3001 ggttatgttc ggactgcatc aaattttgac tccaatccat gtgcaaatga ttgatgaaac
     3061 tggtcgtacg ctattgcttc caattcttgc gatggctggt gcaggacaag tgggtgcagc
     3121 attggcatta tggattcgct gcaaaaaaga taaagaatta acagaaatga tcaaaggagc
     3181 tttaccagtc gggatcctag gaatcggcga accattgatt tacggagtaa cattacctct
     3241 aggacgccca tttattactg cttgtatcgg tggaggtatc ggcggggcag tgatcggaat
     3301 gatcggaaat gtcggggcta tcgctattgg accttcagga gccgctttga tcccattgat
     3361 ctctgacggt aaatggttag gctacatcct tggattattg gcagcttatg ctggtggctt
     3421 cgttgctaca ttcttcttcg gtattcctaa agaccgttta gaaaaagaag aattagcgac
     3481 tgaaacagcc acagtaaatg aagtgcctga aaaagtagta cctcaaactt cagctgaaag
     3541 tgtgactatc ctctcatcag ttgctgacgg aacagttgtt tctttagata aagcaagtga
     3601 tccagttttt gcacaaaaga tgatgggtga aggctacttt gtagaaccag aaaacggaca
     3661 tatctattct ccagttactg gggtagtcag ctctgtcttc ccaacgaaac acgctattgg
     3721 gatcacaacg ccaaatggtt tagaaatctt gcttcatatg ggaatcaaca cagttgactt
     3781 aggcggtacg ccatttgatg tgaaagtaac agaagggcag caagtgacac ctgatacgct
     3841 tgtagcagaa gctgacttag aagcgatcaa agcagcaggc aaagaaacat ccatgatcgt
     3901 cttggtaacc aatatggatc gtgtaaaaaa ctttgtcttg gaaaaaacag gtaaagcaaa
     3961 agcaaaagca cctgtgatgg atgtcaaagc ttaatatgat agaaacaata gaaacgaaag
     4021 aagaaactga aatctatttt tcagcttctt cttttttatt atttattgtt ttctgctaaa
     4081 atctgataaa ttcgtgtcac atcagatggt gttccccgta attcataatc tgccaagaaa
     4141 ggaaagccaa atgtttgtgc atattgtttg gctgttagac aatactgatt attgaagttt
     4201 ttatttccgc taccgactac accaagacaa agtttatgat tgtcttcatg ttccagatat
     4261 tcacgcatcg tttcggtcaa gatttctgta tcaccgttat ctaaaccatt tccgccgtct
     4321 aaataagtag gaacgaaagt aaaaaatggt tctgtttctt tcgtaaaatc actattttca
     4381 tggatttctt ttaaagtaac ttcaggattg acaggatctt ctgcatgcat tttttcagca
     4441 tattctgcta gatgtttggc aaaggcacgc gtatttcctg aaatcgatat atataaaatg
     4501 ttcatggatc atcctccaat tctttccttt tagtatagca tcttttttaa gttttttggt
     4561 ggtgtaattt ggaaagggac taggcgcttt gcttgtataa tgagaaaaaa gccggtagaa
     4621 ttatatttct attaaagttt ttattaatta agtaaaaata aaaaaggttt gttatacttt
     4681 ttgataaaag cggttttatt ttggaggatg cttacatgga tacagagcaa cagaaaaaag
     4741 gaattggttt aattccactt gctgccttag taattggttc agcgattgga ggtggcgtat
     4801 ttggtattat gacggacatc tccggatcgg ctggcggccc agctattttc agttgggtac
     4861 tcgtaggaac agcaatgttg atgctctcct tgtcgatcaa taatatcaat aaaaaacgtc
     4921 cagatttaaa aggcgggatc tttagttacg cagaagaagg ttttggccgt ttcgctggtt
     4981 ttatcagcgg ttggggttac tggctcacat gctggttagg caacgtagct tttgcaacat
     5041 tgttgatgag cgcattagga tatttcttcc cagtatttga aggtgggcaa aatctggcat
     5101 cgatcgtgat gagtacagtc tttttatggg gatatgcatg gctagtgaat cggggagtcg
     5161 aaaatgctag tttcgtcaat gctatcgtca cattctgtaa attagtgcca ttatttttat
     5221 ttattgtagt agctatcagc acgttttcga tccaacaatt cattgataac ttttctagcg
     5281 acattatatt gaatgcgaat ggacagccga tcccgttttc cagtcaagta ttgagttgca
     5341 tcatggtgat gctttgggta tttgttggta ttgaaggagc ctctgtcgta ggttcacgag
     5401 caaaaaaaca atcagatgtt gggaaagcaa cgattctagg gatattcggc ttgattatta
     5461 tttacgtctt ggtttccatg cttccatatg ggacaatgac gatgaatcag ctgcaaacga
     5521 tcaaggcgca gcctgctatg ggctatgtgt ttgaaatgat ggttggtaaa tggggagcag
     5581 tcgtgatcaa cggcggtttg atcatttctt tagttggtgt ttggctctca tggacgatct
     5641 taccgattga aacaatggga aacatggctg aagacggact tttgcctaaa gcatggggaa
     5701 aactcaacaa aaatggggca ccaacttatg ctatcgtatt gacgacgtta tgtacgaatg
     5761 tctttttact ttcattatta ttcactgaca gtgcttataa ctttgcttac acacttggta
     5821 cagcagctat tttcttcacg tggcttttct taggattgta ccaaatgaaa ctttcttatc
     5881 agcggaaaga atgggtccaa tttttagtag ggcttttagc aagtgccttt caagtatggg
     5941 ctatgctagt cgtagccttt aatgaagtaa tgcttgttct tgttcttttc ttgccaggca
     6001 tttattttta cctcaaagca cgtaaggagc aaaacgagcc tgtacaacat atccacttag
     6061 ataagatttt acttataggt gtgacgatgg tcggagtgat tgcaatggtt ttatttgcca
     6121 ctgggatgct tcacgtatag aagtataaaa gaagtataaa gagaggttgt gagcctgtcg
     6181 tttattcaaa aacatactag gaaaggagct gtgacacaaa tgttgtcaca gctccttttt
     6241 cagaataaac ggtgtttaag agacagccaa tcggcaataa ggctgaacgt ctctttcaca
     6301 gcctcttttt tgatgagtca tcatacattt tttcgcttaa atacgagata tcctaatgct
     6361 aagaacaaaa cgatgtaagc taaattaccg aatagcattt gattagtaga aagatgagtc
     6421 aattgcttca tgatctcttc gtttccaatt tggttttgca gattcaacat actgatcgga
     6481 ttccatttga tccactccca tttttggatg agtgcaataa ggataccaga tatgattgac
     6541 gaagcaaaat aaaagacgat tcctgctgag atggatgcgc cggaactatt gataaagcaa
     6601 gcaatcatca acactaaact taaaatcagc cataaaccga tataactacc caaagtataa
     6661 gtgaacaagc tttggatcag tgtttgacca gtagaaacct tctccgtgaa ggaaatcgaa
     6721 gggaaaagaa tcagtttcat caacacagtg acaataatag ctaaaaggta taaataaaca
     6781 gaataaataa ctaatgtgat ccatttgctt actaatattt ctcctcgaga atactttcga
     6841 taaaggagat ttttcaatgt accatattgt gcttccatcg aaatgatcgt gcttgcagct
     6901 gcaatcatga taaagacaat ccaagatgtg gcactaaaaa gctgagcagt catttcaact
     6961 ggatcaatga tcgtcgtgta ttttttggct agaagtgctg ttcctatgag caaaacaact
     7021 aatagaacag ataaaacagc agttgacttt ttcttgatca atttaaataa ttcttgtttt
     7081 acaagcaatg tcatttttct tcttccccct ttactgaatc cagaatttcc aatagagaag
     7141 attccaaatc attcgtcaga tgctggacgt ctgtcacatg gatattttgt gcagacaata
     7201 gctggatcac ggttcctaat aattcttcgt tttcttttaa aagcttgatt ttatcaggat
     7261 cttccaaagt atatccagct tctgtcaaga tcgttcgagc agctagatca tttgttgtag
     7321 agataacgat gaaatcttgc gctgtttggt tcaattcagc cattgtacat tgtttgatga
     7381 tctttccttg attgatcagg atcagctcat cgaccatttt ttcaagctcg ctgagtaagt
     7441 gactggagat caagaaagag gtgccctgag tagccaagtc ggtaatgatt tttcgcacat
     7501 ctcttgttgc ttgtggatcc aaaccgttca tcggctcatc taaaataacg atagacggat
     7561 ggttcagtaa ggcaagagca attcccaatt tttgtttcat tcccaacgag taggatttcg
     7621 cttttttgtg aatgtacttt tccatcttga gctgattcac gatagtgtcg atagcagata
     7681 catcgttggt ttcagagaat aacttcagat gatcatagcc tgttaagaat ggatagatac
     7741 ccgggtattc gatcaatgct cctacttgtt ccaacccttt atgactagag ggagaaatct
     7801 cattttcacc aatacagatt gttccatctg agtaatggat cagtcctaaa atagctttca
     7861 tgatcgttgt ttttccagcg ccattaggac ctactaatcc aacgattttg ccaggggata
     7921 agctaaaact gacatcgctg agggcttgtt tgtgaccaaa gttcttggat aattcttgga
     7981 ccgaaagact tttcaaaaat attcactcct tttctataga tagtacaagc atacacctga
     8041 tgagtggaga agtcgctagg tcattggact tatttcttat tttttcataa aaaaattgaa
     8101 tattaaataa aaaggcttat tttttctgat cagtacttat tccaagtgtc tagtggccgc
     8161 tagtaatctt gatgccaatg atactgatga gtagcatccc cacaaagaac caagtggaaa
     8221 gattcagctt atcatgaaac aagaccacac caattatgac cgaaccaact gcgccaatcc
     8281 ctgtccagat aggataagag agactcatcg gcaaactttt gattgccttt gacaaaaagt
     8341 aaaaactagc aatcatgcca atcactgtgt aaaaagaata attgatttta gaaaaaccat
     8401 cactccattt catcatcgtt gaccagaata cttccaaaat tcctgctatg attaattcca
     8461 tccatgccat acttacattc ctccttaact ataaaaaaat acacaaaaag aggaaaatca
     8521 tcccttcaaa gacctaatgg aatgatcatc ctctttatcc tacccggtgg cagattgtat
     8581 atctttttct gtagtatagc acacctatcc agatatcggc aatctcatac tttttagtaa
     8641 agtgtaccaa gcactgacgg atatatcgtt agcggaaaag aagatgaatg ctttgcaatt
     8701 tcatagaa
//
LOCUS       DACCXM010000108         8574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCXM010000108 DACCXM010000000
VERSION     DACCXM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.111"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(172..2553)
                     /locus_tag="IVY61_002248"
     CDS             complement(172..2553)
                     /locus_tag="IVY61_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7971324.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2543..3190)
                     /gene="recU"
                     /locus_tag="IVY61_002249"
     CDS             complement(2543..3190)
                     /gene="recU"
                     /locus_tag="IVY61_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP7971325.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3254..3793
                     /locus_tag="IVY61_002250"
     CDS             3254..3793
                     /locus_tag="IVY61_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP7971326.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3883..4302
                     /gene="gpsB"
                     /locus_tag="IVY61_002251"
     CDS             3883..4302
                     /gene="gpsB"
                     /locus_tag="IVY61_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP7971327.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4324..4691
                     /gene="rnpB"
                     /locus_tag="IVY61_002252"
     ncRNA           4324..4691
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVY61_002252"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4900..6066
                     /locus_tag="IVY61_002253"
     CDS             4900..6066
                     /locus_tag="IVY61_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP7971328.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6112..7608
                     /locus_tag="IVY61_002254"
     CDS             6112..7608
                     /locus_tag="IVY61_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP7971329.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7689..8372
                     /locus_tag="IVY61_002255"
     CDS             7689..8372
                     /locus_tag="IVY61_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7971330.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2685 a         1565 c         1824 g         2500 t
ORIGIN      
        1 aagaaaaatg gattttctaa tttttaagct aaatgttaga tcacacatgt atatgctgga
       61 tagtcttata aaaaatcatg taatacagct tggtacatga tttttatttg tagctgtttt
      121 ttacatagac acaaaaaaga aaccacgctt gtagacgtgg tttctttttt actatctgtt
      181 tccagaagct gaggtgctgc tggatggtgt atggtcattt gcagcatccg ctccgctagt
      241 tgctggctca ctggaagaag caggaggagc agatgaagac ggtgtcgagc tttcttcgga
      301 tgaagaagaa ggaaccgttg ttgaagacgc tggctcgctt gtttctgctg tcgtgctttc
      361 ttttgaggac tcagcagttg attcgctttg actagtagag ctagagcttt gagtcgttgt
      421 ctctgttgtt gagcttccag gagtctggac atagctggaa gatggaatag ttgttgatgg
      481 tgtaattgca ttcgatctag ctgtatattg atctttgtag tacagttcac cgcccacacg
      541 aattaatccg cttggcatct cccagtctgt atttgtcacg ttagctgaaa catactgcat
      601 aagctcgcga taaacatcag aggctacgtg tgacgattct gaagtgacag gcgtcatttt
      661 tttattgtaa cctgtccaca cggaaatcga ataatttggc gtatagcccg caaataggat
      721 atctggatac accccagaag aaataccaag ttttgcatac tcgtcatctg tgtaattgga
      781 tgttccggtc ttaccggctt gatataaccc agcgatttgc gcattcgtac cagttccttc
      841 agtgatcgta tcttttaaaa tgtctgtgat catataagct gtttctggag acatggctgt
      901 ttttccatct ggctcataag tttcttctgt tccgtcttgg aagacgattt tattaacata
      961 ttgaggtttg taataagtcc ctccattggc gaaagccgca taggctgctg ccatttttaa
     1021 ggaagaagct ccgtattttg tcccatcttg ttcttccgta ttactagaga tcgcattcga
     1081 ttgatggatc gtactgtatt cgatcccaag attttttaag aaagaagcca ctttatctga
     1141 accgacttca ttgaataatt tgactgctgg aacattacgg gataagtaaa gtgcttggcg
     1201 taaagtaatg gtgcccatat attgattatc ccagtttcct acaggagtcg aagtcccttc
     1261 atagttatac ggtgcgtcag taatagtttt tccagtagaa tatttcaaat attcaaaagc
     1321 tggtccgtaa tctgtgacag gtttcatcgt agaaccaaaa tcacgggaag tattcactgc
     1381 taagttgttg cctaaagtta cgtcttctgc gatgtgacgt ccgccaatct gggcttttac
     1441 tttcccggta ttggtgtcga taagggtgga ggcaacttgc atctcgtcgt ctgggtaaga
     1501 gacatactga tccgtgttga cgatatcata tagtttcttt tgggcgtcca gatccaaatt
     1561 cgtataaatc tctaaaccgt ctgtgtagac attcttatct gttttttctt gtacttcatt
     1621 gatgacttct ttgacataat tatcgacgat cttagtattg tcatcggatt gagttagttc
     1681 ttgcagacca tccgttaccg gtacattaac agcttgatca tattctgttt gagagatttt
     1741 ttcgttttga agcatcgtat acaaaacagt atctcgtctt ttcttcgcct gatctggata
     1801 aacataagga tcataggcgg aaggggcttg aggcatccca gcaagcaatg ctgtttgcgg
     1861 taaagaaagt tctgataatt tcttaccaaa gtacatctca gaagctgtct ccatcccgta
     1921 caagccgttt gacatataca ctttatttac gtagtaggtc aagatctctt gtttcgattt
     1981 tttttgttcc aatcttacag ccatccacgc ttcttgggcc ttgcgtttca gagtctgatc
     2041 ctcggcactg gtggagaaga aggaaagttt gatcaattgc tgtgtcaaag tacttccgcc
     2101 ttgcagtccg ccagaggtaa agttagagag ggcagaaccg atgatccgaa tagggtcaac
     2161 cccgatgtgt ttataaaaac ggcgatcttc taccgacacg atcgcatctt ctaatgtttt
     2221 cggtaattca ttcgcagaaa ttttttcacg cttttctgcg cctaaatctt cgaatagttc
     2281 gccatcttgt gtatatagtt tggaagatac ggtagcgtct aattttttat ccgtcaattc
     2341 gggcgcatct tttgcgtaat accagaaaag tcctacacct gctaaaaaga ggatacagaa
     2401 aaatgagagt attcctaaaa ggatcttgat aaataatcct tttttatgtg ttccagagga
     2461 ctttccagaa cctttgccag aattcttctt cacactcttt ttaggagtag gctgtttctg
     2521 tcgtctagag cttctagttt gttcatttgc cattcttgct ttctccttca atagataatg
     2581 ttgataaata gaagtcaacc gcgtccaaat aaggaatccg cggagaaata ccagttttga
     2641 tctctattcc tagttcttcg atcaaagata aaggtagtga tttgcgtcct ttttcctttt
     2701 gggcgtccca gtaagcaatc aaatgaacac tattcaaata aaaacagcgc ttcaaagacg
     2761 aaaaccataa caagacaaag cagaccccat tttgttcgag gcatgctttc atatgttcga
     2821 tttgatgggc atggaagttt ttaaagggaa aagatgtttt gttcttggtt tcttttgctt
     2881 caaagtccaa gtaaaagcct cgatagacgc cattatagtc tgtagttgag gcttgggtaa
     2941 agtaggcttc cttgatcaca gcagcacttc tcttaggata gtctactttc acgatttgga
     3001 taggggtagg ctttttatgg ataaccgcca acccgcggga aagataatat ttattacttt
     3061 cattgattgc ttcttcaaag cgcatccccc gattgccgaa ttcagtgatc tgatttcttt
     3121 tttcttgaat tttttcgacg ggagaggcat tcttataata aggttggcca tttggatagc
     3181 gcaataccat ctttaatcac actcctaggc cacattatag caaaaaagcg gatagaaaga
     3241 aaaggaatct gagatggata agtcaaaagt aatctatgtg tctgggtatc gaagtttcga
     3301 actcggtgtt tttaaagaaa atgatcctaa aatccaagtt gttaaaaatg ttttaaaaaa
     3361 agaactccat cagttagcag aagaagggct ggaatgggta cttgtcagtg ggaatttggg
     3421 cgtcgaacta tgggctgtgc aaatagtagc ggaattgaaa gaagaatatc cggaactatc
     3481 tcttggtatt ttgtatcctt tttctgattt tggtaataac tggaatgaag ggaaccaaga
     3541 aaaaaaacaa ttagcagaac aattagcaga ttatgtcgaa gctgttagcc atcaacccta
     3601 ccaatcacct agtcagctaa agaatcacac tcgttttata cttgagcata cagtgggctg
     3661 tctgctgatc tatgatgaag aattccctgg aaaaacgaag tatttttttg aagatgcaca
     3721 gaattaccaa gcagatcatc cctacgaaat tcggctgatc agtatggacg atcttcaaaa
     3781 ttttagtgaa taatcgatat tgtgtttgat ttatcaactt ttttcctata caatgaaaaa
     3841 ggtagaaatg ttattacgta aatgaaaact agaggtgtaa atatggcgaa tttggtttat
     3901 agtcctaaag acattttaca acaagaattc aaaacaaaaa tgcgcggcta tgatcccgtt
     3961 gaagttgatg aattcttaga caatatcatc aaagattatg aaacttacag caaagaactc
     4021 cttgctttac aagaagaaaa cgatcgattg agtgcaaaag tagctcaatt atctaaaact
     4081 caaggagcag ctcaaacacg cgtgcaacaa acagaggcac caaagagcgc agcagtgacc
     4141 aattttgata tcttaaaacg cttgtcaaat cttgaacgtg aagtatttgg taaaaagctt
     4201 gatcaacaag caagtgcagt caaaccagca caacctaatc caaataacta cacaaatgca
     4261 gacacaagct tagatgataa tgaaaaaaca cgtcaatttt aatttttgat taatgaattt
     4321 gcggttttcg ggtaatcgcg gtctgacttg atcaggctga ggaaagtcca tgctcgcaca
     4381 ggctgtgatg cctgtagtgt tcgtgcttag cgaaaccata agctaaggta ctctttggag
     4441 taacggcagg aaaacaagct aaggcttcgg ctatgcttaa gtatccttga aagtgccaca
     4501 gtgacgaagc ggctggggaa acccatccgg tggaacgcgg taaacccctc gagcgagcaa
     4561 cccaaacaat ggtaggggcg ctttttgtct ggaagaagaa caggacaaaa aggcagtata
     4621 actgcagata gatggttacc gctttgtttt ttctccctgg aaaagcaaag tacagaacat
     4681 ggcttataga aaaccgcaaa catcagggaa ttaggtcgtg acaaaagtaa tgcgttataa
     4741 gaaacaagaa aaacatttga aagctatttt tcaatacttc agatgaatcg ttcttattac
     4801 aaaacaaact tttgtatcac cggttattcg gaatttgagt aaaagtgtgg ggtaggactg
     4861 atgtcccacc tccttttttt ataaataagg agattgtgta tggaaaaaaa caggaagttc
     4921 aacctcgtcg caactgcggc aagcgggcta gaagcactgg ttggtaaaga attaagagat
     4981 cttggaatcg aatgccaagt tgagaacgga cgtgcacgtt ttgaaggaac aatggaaacg
     5041 atcgctacag ctaatttatg gttacgtaca gcagatcgag taaaaatcgt ggttggggaa
     5101 ttcgatgcgt atacgtttga tgaattgttt gaaaacgtaa aagcattgcc ttgggaagat
     5161 tttcttccac tagatgcagc ttttcctgta gctggaaaat cgatcaagtc gaaactatat
     5221 agcacacctg attgccaagc gatcacaaaa aaagcaatcg ttgaacgtct ccgtacctat
     5281 tatcaccgtc cagcttctgt accactgacg gaaactggcg cacattttca gttggaagta
     5341 gctttgctga aagaccatgt gatggtaact ctggatacga caggaccaag tttattcaaa
     5401 cgtggttatc gcttagaaaa aggtggagca ccattgaaag aaaacatggc agcagcactt
     5461 attatgttga caaattggcg aaaagaccgt ccattttatg atcctgtatg tggttcaggt
     5521 acgctttgta ttgaagcagc cttaataggc cataatatcg caccaggatt caatcgtgag
     5581 tttgcttgcg aatcttggga ttggttcagt caagaaatca tggaaaatgt tcgatcagca
     5641 gccgaagaaa aagcagatta tgatattgaa ttagatatta ctggttcaga tatcaatgga
     5701 cggatgattg aaattgccaa agccaatgca gaagaaatcg gattaggctc ttcgattacc
     5761 tttaagcaac aagcagtcaa agacttcaaa acggataaag aatacggggt catcgtggct
     5821 aatccgcctt acggagaacg tctaggagaa gaagaaactg ttcggaaatt atataaagaa
     5881 atgggcgatg tattccgtcc attaaaaaca tggagtaaat atatcttgac tagcgactta
     5941 gcgtttgagg aattttacgg acaaaaagca accaaaaaaa gaaaattgta caatggtgca
     6001 ttgcgtacgg atctattcca gtattgggga actcgtccgc ctagaaaaaa agagaaagac
     6061 gaataatctt actaattgat tcattcaatt ggatgtttgg aggaataggg aatgaaagaa
     6121 aaagagtttt tacaagaagt aaaagaaatc aatcttctac aacaagcttt aggaatcctc
     6181 gattgggata cacaaacagg tatgcctgaa aaagctagtg cataccgaag tgaagtagac
     6241 agctatcttt acagcattta tttttcaaaa aaaatcggtc caaaaatcaa agaagccatt
     6301 cgctattttt ctgaacatcc ggaagaattg tcggaagttg gaactgccgt ttttgccaaa
     6361 gtaaaagaag agtatgattt ggaacatcgc gtaccggaag atcttatgag aaaatataca
     6421 gctgccgttt cttcagcaca cggacaatgg caaaaagcta gagaaacaca acaattttct
     6481 gattttcaac acgcattgaa tcaaaatatt gaattaacaa aacagttgat accttactgg
     6541 agaaaagatg aaaagacgaa ttatgatgtt ttgctgaatc aatacgaacc cgggatgact
     6601 gtcgaaatcc ttgataaggt atttgatcag cttaaagaag gaattctttc gatccgccgt
     6661 acgctccaag aaaaagggac tgaacctgaa acagactttt taaatcggag aatgacgaaa
     6721 aaacaacaga agagatttgt agtgaaagta atcgaacaac ttggttatga ttttagtcgt
     6781 ggtcgtttag atgacactgt ccacccattt atgactggaa ttaatccgaa tgatgcacga
     6841 atcacgacac gatggaacga aacagatttc aaaatggctg tttttggagt gatccacgag
     6901 gctggtcatg gtatgtatga acaagacatc gatgataaat atgcgtatac accggtttat
     6961 gaaggtacat caatgggtat ccatgaatca caatcgttgt ttaacgagat cattattgga
     7021 agtagtcgca gcttctggaa aaagcaatat cctttctttc aagaatgtgc agaaggaaca
     7081 ttcgatgata ttccttttga caaattttat cgttcattga accatacaaa atcaagtttg
     7141 gttcgtatcg aggcagacag tttgacttac cctctccata tcatcattcg ttatgaaatc
     7201 gaaaaaatga ttttcaatga aggggtagat gttgctgaac tacctgaaat atggaatcaa
     7261 aaatatgaag agtatctggg aatacgacca acaaatgatg tggaaggcat cctacaagac
     7321 gttcattggt caggtgcaag ctttggttat tttccctctt atgcattagg gtacatgtat
     7381 gcggcacaat tacttcattc tttgcagcaa gaaatagatg tggaaaatgt tctagcgtca
     7441 gacgattatt ctccaatcaa aaactggatg tcagagcata tccatcaata tggtgcatct
     7501 agaaaaccaa atcaattaat catggatgca acacatgaac cgcttaatcc gcaatacctg
     7561 atcgactata tgaaagacct gtattttaat gtatatcagg taaaataaaa tacaatttac
     7621 aaagatagct gaaattcatc taatctatag atgttttcag ctatttactt aagaaagaag
     7681 tggaacgaat ggataaatta caagtgatcg ctgattatag tatggaccaa ttagcgcatg
     7741 atcaaacagg acatgggagc gatcatacca aaagagttgt caagttagct gaaaaaattt
     7801 tggagacgga accgcaggca gaccgctttg tgacgcttgc agcagcttac ctacatgata
     7861 cgattgatga taaagtcgtt aaaaatgaga acgaggcaaa gcaacagctt cgtgtattcc
     7921 ttcgctcttt gccaatagta gaggaacaga tctcgatgat tttttccatt atcgaaaata
     7981 tgtccttttc taaaaatctc tctgaagcgg ccgaactatc tctggaaggt aagatcgttc
     8041 aagacgctga tcgtatcgaa gcattaggag caatcggcat tttgcgtact gcttatttcg
     8101 gcggtggaca tgggcacccg atttttgatc ctgaactgta tccgcaaaca ttcaaggaca
     8161 aagaaaatta ccgaaaaggc acgacagtga tcaatcattt ttatgaaaaa ctgttcttgc
     8221 tgtctgataa aatgaatact gattacggat atgaagaagc caaacgaaga gaaaccttca
     8281 tgcgtgaatt cttagaagaa ttttttctgg aatggtctat tgacgatgaa aattttactc
     8341 cagacaattg gatcaaaaat aacaaaaaat gatggaggtt gtgacagaag tggtcagctc
     8401 caagatattt cggatcattt tccgtaaaag ttacttctgg aacactgttt accaagaggt
     8461 cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tcaaaaaata gcttctcgtg
     8521 tttgatgaat attgtaactt gtgattcgga attggcagga gaacaccgtt tatt
//
LOCUS       DACCXM010000109         8569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCXM010000109 DACCXM010000000
VERSION     DACCXM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.112"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..851)
                     /locus_tag="IVY61_002256"
     CDS             complement(<1..851)
                     /locus_tag="IVY61_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7971331.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTI"
     gene            997..1800
                     /locus_tag="IVY61_002257"
     CDS             997..1800
                     /locus_tag="IVY61_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP7971332.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1978..2925
                     /locus_tag="IVY61_002258"
     CDS             1978..2925
                     /locus_tag="IVY61_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7971333.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2993..3286)
                     /locus_tag="IVY61_002259"
     CDS             complement(2993..3286)
                     /locus_tag="IVY61_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP7971334.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(3314..3979)
                     /locus_tag="IVY61_002260"
     CDS             complement(3314..3979)
                     /locus_tag="IVY61_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP7971335.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4119..4751)
                     /locus_tag="IVY61_002261"
     CDS             complement(4119..4751)
                     /locus_tag="IVY61_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7971336.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4758..5825)
                     /locus_tag="IVY61_002262"
     CDS             complement(4758..5825)
                     /locus_tag="IVY61_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7971337.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5822..6553)
                     /locus_tag="IVY61_002263"
     CDS             complement(5822..6553)
                     /locus_tag="IVY61_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP7971338.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6722..7201)
                     /gene="greA"
                     /locus_tag="IVY61_002264"
     CDS             complement(6722..7201)
                     /gene="greA"
                     /locus_tag="IVY61_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP7971339.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7337..8512)
                     /gene="mltG"
                     /locus_tag="IVY61_002265"
     CDS             complement(7337..8512)
                     /gene="mltG"
                     /locus_tag="IVY61_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP7971340.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         2545 a         1764 c         1330 g         2930 t
ORIGIN      
        1 tggatcgtca catgtgtaat accatatttt aattttaata aagaaattat acgaggatat
       61 atcttttcaa tctcgcttag tagctgatct tcaaaatcaa tatgtccgtc gaaaataatt
      121 atcttttcgc tatactgcca aatatggaca tgatgaacat cctttacccc atcgattgct
      181 tcaatttctt gttccatttt ttcatagtcg atagcaggtg cactttgtat caaaatagcg
      241 gcagcttttt tgatcaccat tatcgcttct atcaatatat atatcgaaat gatcagtgta
      301 ataactggat cgatccagta gatatcgaaa aaacggatca aaattgctcc gataatcaca
      361 cttaccgaag acagtgtatc acttaacaaa tgcaaataac tagattttat attcaaactt
      421 tcatctgctt cttgtcgtaa taataatact gagaacaaat tcgcgagcaa tccaataact
      481 gctactgtta gcatcaaatg actatttatc ttttctggtg aagtgaaccg cttgaatgct
      541 tcaacggtta aaaacaagga taatatgatc aatatgaatg agttgatgaa agcactcaat
      601 atttctaaac gctggtagcc atacgtacgt tttttatttg cttcttttcg ggccattttt
      661 tgtgccccgt aagaaaaaat aattgccaat gtatcactaa ggttatggaa agcatctgaa
      721 acaagagaca aactccctga cataatccct ccaataaatt ctgataaagt gatcaccgca
      781 ttcaaaacca ctactccaaa tagttttttt gatgatctct gtggctgtgt aggcactatt
      841 ccttcctcca ttttatttat attggatagt ctttagacaa gaataacatt tttttgatat
      901 ttcaccaatc attcagctgt ttccttaaag cacttgtatt tcacttgata cttcgctaga
      961 attgcaagtg atgacactac tagatgagga gtacttatga caacaaaaca attaattatc
     1021 agttccttta tacgattcaa agacctaaag gaggcgagaa atgtcccatt ttggaaagcc
     1081 attgtctatc ttttagtttt gagtataatt atggctttgc caatctcttt tcaagttttt
     1141 caagtcttaa acaacattca attggatggg caaaagattg ctactcgcat tcctgatttt
     1201 acaatccaaa acgggcagct tcaaaccgaa gaaaaagaag gattcattta ccaaacaaac
     1261 tccattatct tcacatttga tccagaggga aaacgaacag aacaggacat ctctactgat
     1321 ttgatgggga attttgttag tgtcgggatg ttaaaaaaca aacttatcat agctcttcct
     1381 aatacaggca cgacttctgc tttgttgaac aataaccaat tagaacttcc ctacaccaat
     1441 gaatctttaa aaaacttaac aggtaagcag ctccgttctt ttttaagtga agcatctata
     1501 ccactttgga taaaagtcct gacatttata ttttctattt atcctagttt tctgaatctg
     1561 ctgatcactt tgctatttac caacattgct gcttttctct atgcacgctt tcgactaaca
     1621 aaagctactt ttcttgattg tttaaaaaca ttgatctatt caatttcatt gcctactata
     1681 attgcaacaa tcttgatggt ctttcttcct tcttttgaca ctagcgcctt cattgctttt
     1741 gcaggaatct ttatttttgc tcaagctgtc aaaggatggg caaaaatatc gatcaattaa
     1801 gaaataaaga caactcgtta tttctacttc aatagaccct tttagaggtt gtgacatttt
     1861 tttgtcacag cccctttttt atttgaaaaa tatttctttc tcaaaaaaca agcaaggcgc
     1921 ttcaaagttc ttcttgtatg cgctacactt agagtaaaga aaggcgggaa attaactatg
     1981 tcagaaagaa tcatttatgc agatcgtcca tttcgtgatg aatttatcga agaaatccat
     2041 actatagcgc ctggttatat ctttaaaaca gaattacaac cagaggatct acaaaatgta
     2101 gagatcagtt tgggctggaa ctcagactac caagaaaaac tattagcctc ttctaccttg
     2161 aaatgggtcc aatctatctc agctggtgtt gatcagctac cccttgagac tttttcaaag
     2221 aaacatattt tactttcaaa tggtagtggc atccacagtg aatcgattac ggagcatgtc
     2281 ttaggaatca tccttgggta ttctcgtggt ctttttcaag cacaaaaggc acagagtgac
     2341 atgaaatggt taggttcatc ggtccattac caatctatcc aagggaaaaa aatactaatc
     2401 attggaactg gccatattgg aaaaatgatt gcaaaaaaat gcaaagcatt agacatgaac
     2461 tgctatggaa tcaatacatc cggtcatcca gcagaaggaa tggaaaagac tttttcttta
     2521 aatcaactaa aggaagttct tcctgaagta gatttcgtag tcaatatctt acctctaacg
     2581 gaggaaacaa ctgggctctt tgatcagcat atgtttgaac actttgatcc aaaatctgta
     2641 ttcatcaatg ttggccgtgg tgcttctgtt aagacacaag atctggtgca ggctttaaat
     2701 aaccagcaac tttcctttgc agcattagac gtttttgagg aagagcctct tccacaagat
     2761 catcctcttt ggaaaatgga caatgtactg attacttccc acattgcagg tttgacacct
     2821 gattttcaaa agaaattaat ggctattttt ttaaccaatc tgaaaagcta ctttgccacc
     2881 catgagcttc aaaccaatca agtaaaattg tctgcaggat attaatagct gaaaacaata
     2941 ttttcaaaag aaaaaagaga aggtgacaaa aattgtgtca ccctctcttt ttttattgtt
     3001 ttttaaagtg ataggttggc tcctccaggt tgggattata atcaacaagt aaatcatatc
     3061 ctttaaagaa ccattcatct gcttcctcga taaaaaaaag tgtcccatca atttccactt
     3121 tctttacagg atgttctggc tgttcaactg acattccaat cgaaaaacca tcatgaacct
     3181 ctgttttgcc gtagactttt ccaaaaaaac gtattccgta agtttctggc agatcaattt
     3241 ctgagtcaaa ccacttttgt gcttttggcg ttaccgttaa tttcatccca acaacctact
     3301 ttctcttttc tttctattga ttcatcttat cgtctagaat atccacatcg cctgtctctc
     3361 caataaccag tagattatca ttttcacgaa catattcatc tgctgcagga gaagcgataa
     3421 ccgtttgatc tactcttcgt atggcgacta ctgttaagtc aaaacgttgc ctgaaattta
     3481 attcagctaa tgttttgttg aaaaatttag gattggagac tcggatttct gctaaagaat
     3541 atttatctga caactctagg taatctaaaa tattctttga cactaaatta tgcgcaatcc
     3601 ttattcccat gtctcgctcg ggatggacga cgcgatcagc tccgatcttt tcaagtactc
     3661 tggcatgata ctcattttgg gcttttgcta aaatatttgg tacgcccatt tcctttacca
     3721 tcagagtaac taagatactg gcttgaatat cttctccaat agcgacaatt acatgatcga
     3781 aatttcttat gcctaatgat cgaagtgtca tttcatcctg tgcatttgcc acaacagcat
     3841 gagtggctat attcatatat tcattgaccc gatcttcatt actatcaata gccagtactt
     3901 cttgaccagc ttcgatcagt gttcggcaga tacttccgcc aaatcgtcca agacctacaa
     3961 ttgcaaaatt ttgtttcacc gttttcctcc tttagtatct tcttttctca gtataacaaa
     4021 gcctttacaa aaacaaaagc aagcatcatt attgtaaaaa agccaagaaa aataatagtt
     4081 attttctcag cttttgatta tcctctttat catcgttttt atttcaccaa accatgtttg
     4141 aatgcataga tagcggcttg tgtacggtct tccacttcta gcttagccaa tatattggaa
     4201 acatgtgttt ttactgtttt cagtgtaata aacaattcgt ccgctatttc ctgattactt
     4261 tttccttcag aaatcagcat taaaatttca ttttcccgat tcgttagttc ttcatgtaat
     4321 acaggctcat ttcgccgact cattttttcc atcatttttg ttgtcacttc tggttctaat
     4381 actcgctcgc cacgttgcgt tgcccttata gcatcagcaa tttcatgtgc ggtagaggtc
     4441 ttcaacaaat accctgctgc acctgcttca atggcaggat aaactttttc gtcatcaata
     4501 aaactggtta cgataatgat tttagcttct ggccaatctt ttaagattgc ttttgttgat
     4561 tcgataccat ccatttcttc catgactaag tccattaaaa tgacgtccgg tcgaagtgtc
     4621 atcgccttct catagccttg tcgtccattt tcagcttctc caattacttc aatgtcttct
     4681 tgaatagata aataaaaaga gacgcctaag cgcaccattt catggtcatc tactaataaa
     4741 acttttatca tccttatcta tgcctccttc atcaaaggga ccttgatttc tacacttgta
     4801 ccttgacctt taaaactaat gatttttact gttccaccaa taccagctac tcgttctttg
     4861 atattgttca atccataact tcccgttttc gtttcattca tattaaaccc tgttccgtcg
     4921 tcaataatac gtaacaaaag attattgtct attttgtgca aatatacctc taattcattg
     4981 gctttagcat gtcttaatgt atttgataac aattcttgaa cgattcggaa caagtgatcc
     5041 tcaatggaac tagatagttt tacatcttct acatcccatt tcagcgaaat ctggatttta
     5101 ttttgcaatt ccttcaataa ctgctcgata ccttgcttta aacttttctc ttctaaattg
     5161 actggacgta gatgaagaag cagcgcacgc atctcagact gggaagcgtt gatgatctct
     5221 gctaccattt tcaactgctt gcgaaacatt tctggtgtct cgcttttttc tgcttgctcc
     5281 gtcaatgccg acatcatcat cattgctgca aacaattgtt gcgaaactga atcatgcagc
     5341 tcacgggcta atcgatgtct ttctaattct aaaatttctt ctttggtttg tccatctaca
     5401 tattggggac ggctcgttac aatttgtaat tcactagata cttcaatcat tttttctttt
     5461 atcttagtga tttccttatc aatatctttg atgtataaat cgtccgatgc acttgggatc
     5521 ggaagaagca acaatttgga ttcataattg ccagaagaaa gtgcacgcag cttttcttcg
     5581 attttcccgt attttgtctt ttgaataagt gataataata gaaagacaat taatcctaca
     5641 cctaaggaaa tggctaacaa atggaagata agcggaacat ataataaacg tgcacgcagt
     5701 aattctcccc accaatgact ttgattgctg gcataaaaat aagtgaaaag tgaaaataga
     5761 ataataagga aagccgcaat ccccgaataa acagctagca tcgctctgga tatttttccc
     5821 atcatattcg tatcacctcg acatctccaa tcaaggtatt agtaataatc ttcaatctac
     5881 gagggttttc atcataatca ctgctgtaga ttttgatttg ttcatttttc aaagaaaatt
     5941 gctcttcttc aaacaagaca tttcctacta atgtcgcatg ctctaatcgg atggctacgc
     6001 ccaaaggtac taagatccgt gttctgccta tcccttttct tacaatcaca atattatcat
     6061 cttttggcaa aagcgtattg cccaaatcta tgatagtatc accagaaatg acggcgatgt
     6121 tgatatcgtc ccattcatag acgtcgtttc caatacgttg gttaccgaat aattgctgtc
     6181 ttttccgctc attattatgt gtttttaatt gctctgtttg gatcatcatg atctgtttct
     6241 ttcgccaaaa agcatttttg gttaaatcta tccctgaaat ctctacacct ttcagaccaa
     6301 taaataaaac ggcaaataca gccatcatcc ataaagcagg actgttgact aagctaaaaa
     6361 aaatgaccaa acttcctaat atcaattgaa aattttgaaa tttagtacga ggatgtttgc
     6421 gcatagctag atatatattg aaaataccga atatcactaa aagcaaaagt ccagtattgt
     6481 ttacgatttg ccaaacagca aataacagca gtagtgcttc aactaccaca aaaaaacgcc
     6541 aagaactatt catcgctctt tctccctcct gacacattct catttcatca tacctatttt
     6601 tttaactgtt ggctatagga ctttagatgt aagatcgttt gttgaaatct aaaatacaca
     6661 aagagaaaaa gatctagagt gtatcatcag taagatgaac tctctagatc tttcagtcag
     6721 actacgcttg ttctactttg acgattttaa ctaacatatc gccacctgga gtagcaatag
     6781 ttacttcatc atttttacgt ttgccaatca atgcttgcgc aataggagaa tcattggaaa
     6841 ttttgccaga aaacggatct gcttcagcgc ttccgacaat cgtgtactct tcttcttctc
     6901 catctggtag ttcgatgaat gtaactgttt taccaattga tacctcatct gcatctacac
     6961 cgtcgttatc gatgatttga gcaaaacgaa tcatattttc aattgttgtg attcttcctt
     7021 caacaaatgc ttgttcatct ttcgctgatt catattcaga gttttcggaa agatctccga
     7081 aaccgcgggc aattttgatt cgttcaatga tttctccgcg tttgactgtt tttagttctt
     7141 ctaattcttt ttctaatttt tctttacctt ctagagtcat tggataaact ttctctacca
     7201 tgtgtaacgt ctcctttttt cgataaaata ccgcaaaagg taatcttttg aggaatatag
     7261 tccaaaaaag attaccacgc ttttcttcaa atgtaaatgt ttttctaaaa atgatcaatg
     7321 tctagagcct gtttttctaa gaattattta catatttttc cactaattcc atatgttctt
     7381 cataagtttt agcgaaataa acatttccag tgctcgtatc tgctacgaaa taataatagt
     7441 cattttctgc tggttctaat actgccttga ttgcctcttc gcttggattg tcaaaaggtc
     7501 caggtccata gccggtattg gtatacaaat tatatggtga atctacagct gtatcttcat
     7561 acgtcactaa ttctttgtgt tcacctaatg cgtataaaac agagatatct gactgaagtg
     7621 gcatatttgc tttgattcgg ttaaagaaga cttgtgcaat gtttttacga tcattttctt
     7681 ttacgccttc tttttcaact agagaagcta gagttaaaac ttcttgtaca gtcaaatttt
     7741 tctgcttgat agctgaataa tagttttgca tgaccgtatt cgtcttgttc accatttcaa
     7801 tcactagatc cttaagcgaa gtactcgtgt aataatcata tgtggctggg aataagtaac
     7861 cttccaaacg gtattttgtg ttctcagctt tagatgcact ttccaacagt tctgggaatt
     7921 tttggtgcaa ctcgttaaag aaagtctcat cattcactaa atctaaaaat tctttcttgt
     7981 ctttaccagt taccttcgct acccgttcag caatctgatc gatatcgtac ccttctggaa
     8041 tggcaatttt cccatctgca acttttgttg gctcagaagt accgccttct tgcaactgtt
     8101 tgccaatctc gtcaagagtc atattaggcg ctaactgata atagccagct tgaaagccag
     8161 ttaaattttt aaattttgta taataattga aaacaatccc gcttttaata atattgtcct
     8221 tctccaaaat ctcaccaatc tgcttgttag aagagccact cgggatctcg acttggacta
     8281 attggtcatc tgatttatct aaaggcttca acccgctatc tacataacga tacacggtaa
     8341 atccaagaaa tccgccaata atcaataatg taagagcaat cactaaaatg atcttcctca
     8401 caatcttatc ttctttttta cgcagatttc tccgcttgtc gattatatct gctgtagctg
     8461 ccttactggc ttctttttta tctttacttg ggtgttgttt gtttcctgac aaaacaactc
     8521 ctccttacta tcaagttcgt aactttattc attatacatg atatttgaa
//
LOCUS       DACCXM010000110         8541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCXM010000110 DACCXM010000000
VERSION     DACCXM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.113"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            74..361
                     /locus_tag="IVY61_002266"
     CDS             74..361
                     /locus_tag="IVY61_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971341.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            777..2315
                     /locus_tag="IVY61_002267"
     CDS             777..2315
                     /locus_tag="IVY61_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP7971342.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2312..3277
                     /locus_tag="IVY61_002268"
     CDS             2312..3277
                     /locus_tag="IVY61_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7971343.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3264..4586
                     /locus_tag="IVY61_002269"
     CDS             3264..4586
                     /locus_tag="IVY61_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7971344.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4649..4753
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4827..5570
                     /locus_tag="IVY61_002270"
     CDS             4827..5570
                     /locus_tag="IVY61_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7971345.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5585..6577
                     /locus_tag="IVY61_002271"
     CDS             5585..6577
                     /locus_tag="IVY61_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7971346.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6589..7326
                     /locus_tag="IVY61_002272"
     CDS             6589..7326
                     /locus_tag="IVY61_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7971347.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7316..8038
                     /locus_tag="IVY61_002273"
     CDS             7316..8038
                     /locus_tag="IVY61_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7971348.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8122..>8541)
                     /locus_tag="IVY61_002274"
     CDS             complement(8122..>8541)
                     /locus_tag="IVY61_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP7971349.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2761 a         1465 c         1839 g         2476 t
ORIGIN      
        1 ttacttcaag aggaaaatga aaaaaaatta tttttggctg tttcttttta tttatcaaat
       61 aaggagtgga aaaatgcgag cagcagactt attgacaatt cttacaaaat cacccaaaaa
      121 aacagagtat caaggagttt ttttggctca agaacaaaaa ctggtgcctg tgacaatggt
      181 ccagataaat aaagcaaatc aatttatctt tttatttgaa cacggcaaag caccacttgc
      241 tatgaaagac atctttcttt ttttgatgaa gaaccgtgag aaagctgttt attatcagtc
      301 aaaagaagaa ctcattcctc tttatggagt gaaagagata gaaaataaag tggtgatata
      361 agtaaaaata agaaaacaag acgtttatga tgtcaaaaca accatttttt acatttttgg
      421 caaaaaagtg tcatgataca gtataatatt cgttgaacag gccaataaag gtgttctccc
      481 ccacgagatt tctactttat tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa
      541 aaaagatata gctgattcca agtgacaatt tcgtatagat ttttatggta ttcgtagtga
      601 aaaaacgaga accaaaaagt tgatccgact ttgttgcttg gattcttttt ttgtcagcat
      661 atttagaggt atttttttct tttttttgtt cgtgctgtaa ggttaaggca agacaagtta
      721 gttgggaaaa aggaaagaaa tgtttatact taaagaataa cagaaaggaa ggtcgcatga
      781 ataattttat acaaacatta agtgaaagaa aaggcgagtt gctggaagct gcatttcaac
      841 atctgtccat ttctctcatt tctttgctga ttgcagcact gattgctatt cctttagcga
      901 tttgggcagc caatcataaa aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc
      961 aaacgattcc ttcacttgcc ttattaggtt tacttatccc atttgtaggg atcggaactg
     1021 ttccagcatt gattgcactt gttatttacg ctttgcttcc gattttccaa aacacgtata
     1081 ttggtctagc tgaaattgac ccgtctattg aagaagcagc ggtagcattt ggtatgtcca
     1141 gaatgagacg cttgctgaaa gtagagcttc ccatcgcatt acctgtcatt atctcaggta
     1201 tccgaactgc cttagtttta atcatcggta cggctacttt ggcagcattg atcggagcag
     1261 gtggattagg gacatttatt ttgttaggta ttgaccgaaa tacacctgtt ttgacattga
     1321 ttggtgcgat cagttctgcc ttgcttgcaa ttatattcag cggactgatt cgctggttgc
     1381 agcataagaa acctcgatat tccatcatta ctttagcagc agtggtgtgt ttatttggcg
     1441 gcgtttcttt atatcagtct gccttgttta aaaatgaaac catcacgatt gctggaaaat
     1501 taggagcgga acctgaaatc ctgatccaaa tgtataaaga attgattgaa gacaacacaa
     1561 aaacagaagt tgaattaaaa ccgaattttg gcaagacgag ttttttgttc aatgcactgg
     1621 atcaaggtca aatcgatatc tatcctgagt tcacaggtac tgtcctagaa agcttggtga
     1681 aggtacctga agcaatgaaa agtgctgatt tgacaaaaga aaaaacatat gacgaagcag
     1741 aagaattatt gaataagcaa ttcgatatga cattattaaa accaatggct taccaaaata
     1801 cgtatgcctt ggcagtcaag agatcttttg cgaacgaaca tcaacttaaa aagatttccg
     1861 atttggcagc tatctctgat cagatcaaag cgggattcac actggaattt attgatcgtt
     1921 cagatggata taaaggaatc caagaagcat atggcttgaa tttttcaagt gtacaaagca
     1981 tggagccaag tttgcgttac caagcaatca ataatggaga tgtcaatatt gtggatgcct
     2041 attccacaga tagccagctc aaggagtacg atctagtgac tctagaagat gatcgagggt
     2101 tattcccagc ttatcaaggc gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg
     2161 aaatcgttaa agcactgaat aaattaagcg gaaaaataac agaggatcaa atgattgata
     2221 tgaattacca agtcaatgtc gaaaagaaaa agccgacaga agtagcacga atcttcctga
     2281 aaaatgaagg attagtaaag gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt
     2341 gcaaaaaaag tatgatggaa agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg
     2401 agaaatcttt gttttggttg gtccttccgg tagtggaaaa acaactactt tgaaaatgat
     2461 caatggatta tctaaaccat cagcaggaga catctacttc aaaggaaaat cgttaaatga
     2521 atataatctg cagaaaatgc ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt
     2581 cccgaccatg acagtcaagc aaaatattga agtcatccct gaaatgttag gttgggaaaa
     2641 gcagaaaaga gcagaccgtg tagatgaact tcttcaaaaa gttggcttat ctcctgatat
     2701 ttatcgagat cgtatgccta gagagttgtc tggaggagaa cagcaacgga tcgggatcat
     2761 ccgagctatt gctgcaagcc cagatgtgat tttgatggac gagccattta gcgcattaga
     2821 tccgatttca agaaactcat tacaagaatt ggttttgtcc ttacacgaag aattgggcac
     2881 gaccatcgtt ttcgttacgc acaatatgga agaagcaatc aagctaggtg atcgtattgc
     2941 atttatgaag gatggagaaa ttatccaatg tgacacacca gagcagttat tgatgaatcc
     3001 taaaaacgac tacgttcggc atttctttga tgaaccgaaa cagacgaaag agtggcgagt
     3061 agaagattta gtcgtcaatg gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca
     3121 agtttgcttt gatacgccta tgaaagaagt atattcgctt ctttcggtgt atccaagcat
     3181 tgtgatagta gaaaagcaaa gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt
     3241 tttaagtcga gaggaggaag gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg
     3301 tgagcggctt aagtgctgct tatggattga aagaagcagg taaaacagta ctagtggtcg
     3361 aagaagatct atggggcggg acttgtccta accgtggatg tgatcctaaa aaagtcttgt
     3421 taagtgcagt agaggcacgt aatcgggtaa aacaattgag tggaaaaggt tttaacgaga
     3481 tcccaactgc caactgggaa gaacttcaaa aattcaaacg aacatttacg gatccagtcc
     3541 cagaaagccg aaaaaaacaa ttagcagaag ctgaaatcga tcatctttca ggaaccgctc
     3601 gttttttgga tgattcatct attgaagtca acgaagaagt cttccatgcg gattacttag
     3661 ttttggcaac cgggcaaaga cctacgatct taccagtgga gggaaaggaa tacttgaaaa
     3721 ctagtgcaga ctttttatca ttgcctgtac tgcctaaaga aatcattttt atcggtggtg
     3781 gttatattgc ttttgagcta gctacgattg ccaatgctgc agggagcaaa gtaaccattg
     3841 tccatcataa tcaacggcca ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc
     3901 atcaaatgga agcatctggc attcagtttg cttttggtgt tgaaacccaa aagatcattt
     3961 cagaaggtac tcgctatcgt ttagtcggaa aagagacgga attagtagca gatatgattt
     4021 tttgtgcgac tgggagacag ccgaatacag aatctttagc acttgagcaa gcgaatatcg
     4081 tctttgataa acacggaatt gcagtcaatg attatctgca aacgagcaat ccaaaaattt
     4141 ttgcgtgtgg agatattgtt tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg
     4201 aaggaaatta tgtagctaag cggataacgg atgcaacaag cgaacctatc aaatatccga
     4261 ttatacctac gatcgtctac gccagtccta aacttgctga agtaggagtt acaaaaagtc
     4321 acgcctcttc gtcagatcaa gtggtagaga tggatcttac cagttggttc acctatcatc
     4381 gtgtcaatga accagttgct aaggctgaat tgacctttga ccagcaaaat tatttgattg
     4441 gtgctgcagt gattagcgag caagcagacg aattgatcga tgatttgaca ctcgtcatca
     4501 atcaaaaatt gactaaaaaa gagttggata gctatatcat ggggtatccg acattggcaa
     4561 gtgatctctc ctatttattg aaataatcag catgataact aaagcgcttg ttgctttttc
     4621 cttcttgtgc tacactttaa aaaatgaata cctgtttctg gggatgtcgt agacgacatg
     4681 agagtaagtt gtgaacttag acccattgaa cctgtcagtt aatactggtg aagggagaaa
     4741 catagcagac gcttgaagcg ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt
     4801 catattagaa gaaaggaggt aacaaaatga agaaaaatct ttatccagca gtcagtatgg
     4861 cattcatttt aattatttgg cagattgttg tcatagtttg gaaaatacct gcctttattt
     4921 tacccggacc tgctgctgta ttttctgcat tagcggcaga tgcacaagta ttactttccc
     4981 attctgtcac gacattaaat gaagcagtgc ttggtttggt aatagctagc gttctttctt
     5041 ttattactgc cttggcaatg gattattgga agtttttaga aatgtctttt tatccgttac
     5101 tagtgatttc acaaatgttg ccaatcatgg tgttagggcc attacttacc ctatggtttg
     5161 gtttcggtat gcttccaaaa gtgatccttg ttgtcttgat aagctatttt ccgatcgtag
     5221 tttctttttc ggatacattg aggaaagtat caaaagagca aatcatcttt ttgaaaacga
     5281 tgggggcaga tacagggaag atttatcgaa tatataaaat accagcgggt ttatccggct
     5341 tcttttccgg tttaaaagtg gctgctactt attgtgtcag cggagcagtc gttggagaat
     5401 ggctaagcgc tcaagcaggc ttagggtatt acatgatacg cgtaaaaaac agctaccaga
     5461 tcgataaagt ttttgctgcg atcatttgtg tcattctatt gagtttattg ctgaatggcg
     5521 cttgttcatt attgaaaaaa gggtattatc gtatgcttta tattttttaa gagaagggga
     5581 tcaaatgaaa aaattatggc ttttattacc gctactgttg cttttatccg cttgtggcac
     5641 tgcaaaagaa acaagtagca aacaagaaat aaaggatttg aaaaaagtga cacttgtttt
     5701 ggattatgta ccgaatacta atcatactgg catctaccta gcaaaagaaa aaggctatta
     5761 taaagaggct ggtttaaatg tccaaatcat cgaaccagga gataattcga caagtattgg
     5821 tctagtaggt gcggacaaag cacaatttgg tgtaagttat caagaagatg tcacttatgc
     5881 tcacgcagat ggacagaata taccagtcaa agcaattgct acagtgatca aacataatac
     5941 ttctggattc gccaccctat cagacagtaa catccattca ccaaaagatt ttgaaggaaa
     6001 aacatatgca ggttggcaaa gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa
     6061 agacggcggg gacttttcaa aactgacaat ggtaggaagc aatggagaag ggccagaaag
     6121 cttaggaaaa tcctcagata tccaatggta ctttgaaggt tgggatatga tcaaagcaaa
     6181 agaagcgggt attgaactaa actatatccc attgaaagaa ttagatgaac ggttagatta
     6241 ctatactccg gtaattatta cgaatgatca actgatcaaa agtgatccag aattggtaca
     6301 gtcttttatg gatgccacga aaaaaggcta tcaggaagct attaaggatc caaatgacag
     6361 tgctaaactg ttgcaaaaat atgcaaaaga aaatgatcgg acgtttttag aagaatcaca
     6421 agcattcttg tcaaaaaatt atacggatga tcctgagaat tggggattga tggaagaaaa
     6481 agtttggcgc aattatacag cttttatgca ggaaaacgga ttgatcaagc aagatgtacc
     6541 ttcgcaagaa ctattcacta atcagtttat aaaataggag tgatagatat gtcaatccaa
     6601 atcagccact tgaatgtagt cattgaaaac ctgaaaatat tagaagacat ccatttcact
     6661 atccctaaag atacctttac atcgattgtc gcgccaagcg gagccggtaa gtcaacattg
     6721 ctgaaaactt taacaggagt ccagcctatg acttctggaa cgatcaaagt agacgatcaa
     6781 aaagtaactg gtctccatac agcatttagt tatatgccgc aagaagatat gttacttcct
     6841 tggctgagcg tctatcaaaa cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat
     6901 gaagaacaag taagggatta tctggatatc tttgggttaa acggctatga acattttttg
     6961 ccggaacaat tatctggcgg gatgaagcag cgaacggcct tgttacgaac aatcatgaat
     7021 ccttcttctt acttgcttct tgatgaacca tttggagcgt tagatgctat gaccagaggg
     7081 cagatgcaag actggatgtt gaagctgcct aaaaaagcaa agcggactac tttacttgtg
     7141 acgcatgata tcgaagaagc aatttattta tcagacagga ttcttgtgct ttccgcccgt
     7201 ccggcacatg tgattgctga gattcaagtg cctgaaaaac gacggtcacg tgaatggcta
     7261 ctgcagcaat cagagttgaa gcaaacgatt tatcagttat tggcaggtga attagatgtt
     7321 aagtgatgct cattgtcatt tagatgggag tcagagttta gcactccttc agcaggaaca
     7381 cacgattttg acgatcatca attgtgattc gccagaagaa tggaaggaaa acagacagct
     7441 tgcagcaagt aagactcaag ccttaagtta cggtatccat ccttggaaag cagatagcta
     7501 tacgtttgaa caagttgagc cttttttgaa aaaagcaagg atcatcggtg aaatagggtt
     7561 agataatatt tggacgaatg taccgatgac tacacaaaaa aaagtatttg agcgacagct
     7621 tgcttttgca gccataaatg agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga
     7681 gatcttaaat tatatccaag cttaccctaa tcactatttg atccattggt attcttcttt
     7741 ggactatcaa caagactata ttgatttagg ctgttatttc agcattggtc ttgatttgaa
     7801 aaagaatcca gcagtctggc aactagctaa agcagtccca atcgaccact tacttattga
     7861 aacggatgga ctacatgcag cagagtgggc tttagggggg attttttcag aaaaagatta
     7921 tgtctcactg cttgaagaac agttaaagac aatcgctgag atcaaagagc tatcagttga
     7981 agaagttaaa aaaaatactt atcaaaactt ggaacgtttc attagattag gtaaataaaa
     8041 aagagatgtg acacaactag tgtcgcacct ctttttggct ttctttgtat aaaagtaaac
     8101 tgtttgatta ataaatgaat cttagtacca accgtttgct tcccagaatg ctttcgccgc
     8161 ttcccaagaa ccataacgtg aagtaacata ttgttctgct actctttctt ggtttgcagc
     8221 agagtagtca ccatttaagt aagaagaatc taattggtaa cgtccgatgt aacgtccgtt
     8281 cgttgctgtg taagaaccgc ttgattcttt ttgtgcgatc cattcttttg cagagcttgt
     8341 gcttgctgga gtagcagcag tttctgttac agcaggagca ggttgtgttt cagcaacagg
     8401 cgctgcttgt ttttccgttt gaaccgtttc ttgtacaaca ggttgttcag cttggactgg
     8461 tgcttcttga acggctgctt gaggttcagc agctgcttgt gcagagcctt ctgttgggat
     8521 tgttaattgt tgtcctgagt a
//
LOCUS       DACCXM010000111         8514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCXM010000111 DACCXM010000000
VERSION     DACCXM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.114"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            126..644
                     /locus_tag="IVY61_002275"
     CDS             126..644
                     /locus_tag="IVY61_002275"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7971350.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            772..1455
                     /locus_tag="IVY61_002276"
     CDS             772..1455
                     /locus_tag="IVY61_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP7971351.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1764..1837
                     /locus_tag="IVY61_002277"
     tRNA            1764..1837
                     /locus_tag="IVY61_002277"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1798..1800,aa:Arg,seq:tct)
     gene            1996..2355
                     /locus_tag="IVY61_002278"
     CDS             1996..2355
                     /locus_tag="IVY61_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP7971352.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2478..4109
                     /locus_tag="IVY61_002279"
     CDS             2478..4109
                     /locus_tag="IVY61_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP7971353.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4171..4812)
                     /locus_tag="IVY61_002280"
     CDS             complement(4171..4812)
                     /locus_tag="IVY61_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7971354.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            5094..5402
                     /gene="rplU"
                     /locus_tag="IVY61_002281"
     CDS             5094..5402
                     /gene="rplU"
                     /locus_tag="IVY61_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP7971355.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5429..5773
                     /locus_tag="IVY61_002282"
     CDS             5429..5773
                     /locus_tag="IVY61_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP7971356.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5786..6079
                     /gene="rpmA"
                     /locus_tag="IVY61_002283"
     CDS             5786..6079
                     /gene="rpmA"
                     /locus_tag="IVY61_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP7971357.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6172..7320)
                     /locus_tag="IVY61_002284"
     CDS             complement(6172..7320)
                     /locus_tag="IVY61_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7971358.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7332..7643)
                     /locus_tag="IVY61_002285"
     CDS             complement(7332..7643)
                     /locus_tag="IVY61_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971359.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(7696..8091)
                     /locus_tag="IVY61_002286"
     CDS             complement(7696..8091)
                     /locus_tag="IVY61_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7971360.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(8127..8471)
                     /locus_tag="IVY61_002287"
     CDS             complement(8127..8471)
                     /locus_tag="IVY61_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971361.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
BASE COUNT         2560 a         1410 c         1648 g         2896 t
ORIGIN      
        1 agatgaggaa atagaattag aaacatataa ttattaaagg gaaacatttt tacagctata
       61 atttgaaaaa atttatcgtt attatatgat taagaaggat tgaataaaga aagaagttga
      121 aaacgatacc tgattacaaa aaaataactt ttataagttc tataacgttg attatatttt
      181 taattcttat tcttctcgtc gtcttggttc cccgaatttt tggtttgact ccttatatta
      241 ttgaggacaa agaaatgagt ccatattatg cacaaggaaa tctgatatat gtacgaccga
      301 aaaatacaaa tgagatttta gtcggagata ttattacata ttatgaaaat tcaggaaaaa
      361 aaatctcgac gcgtcgcgta atagccgttg aaaacaacca cgaagatttt tatacaaaag
      421 ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt atcggtagtc
      481 ccatctttca gattccatat attggactag tcctttcaaa aacggcattt gcatggattc
      541 aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg aatacctttg
      601 tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt taaaaacata
      661 aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa cactcatgca
      721 gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag tatgcgcaat
      781 gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg tacccagcaa
      841 aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc agaagtacca
      901 gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct tcagcctgaa
      961 cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc cacgacaatc
     1021 ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga gaaagctaaa
     1081 aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg tcaagcagca
     1141 gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag tgcaaaatca
     1201 aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg cgttttgatg
     1261 gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat tccccgtggc
     1321 aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga tcatcccgat
     1381 ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa ggaatcagaa
     1441 acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga atcgagggct
     1501 caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga tacaggaaat
     1561 ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg ataaccttag
     1621 aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg ccaaaataat
     1681 tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa ttatctataa
     1741 ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca tccgccttct
     1801 aagcggacgg tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt agaaactgag
     1861 gtttctaacg gtttttcttt gttttttgtg ccaatattgt ttgttttcaa aggcaaataa
     1921 aataaaaaaa catccttatc gagtacaatt ataaggagca aaaaaataaa actcatcaga
     1981 aaagaggaag aagatatgac gatactatct agaattttag tgactctagt tgcaattgaa
     2041 tttttttata tcatgtatat cgaaacggtg agaactgatt ctgacacgac aagtagggtt
     2101 tttaaaatga gtaaagaaga gttaagcaga aaatctgttc aaacattgtt taagaaccaa
     2161 ggagtgtaca atggactatt aggagtggga ctactttatg gagcttatct ttcttccgca
     2221 tctaaagaaa tcacaagtat gctattaata tctatttttt ttgttgcatt atatggcagt
     2281 ttgactagcg atcgttggat tttactcaaa caaggtgggc tagctctgtt ggcattgctt
     2341 tctattttct tataaatata gaaaaaataa gaatttatct aacaggaaac tttgtgaaaa
     2401 atattacttt atctcatgaa atatgatatt ctaatgaatg gtattttaga aaatttcatg
     2461 aaaaaggagt tagataggtg ttcaaaaatt atgtttcctc actaaaaaaa gaattttcag
     2521 gttatgatct ttcaagattt cagaaagatc ttttggctgg gatcacagta gctgctgtag
     2581 cattgccttt agccttggct tttggggtat caagcggagc tgatgcggcc gcaggtttga
     2641 ttacggctat cattgccggt atcgttattg gcggtttatc aggcggtttt tatcaaatat
     2701 caggtccgac aggtgcgatg gcagctatct tgatgtctat tgcggcaaca aagggaatgc
     2761 aaggtgtatt gcttgctact tttttagcgg gtctattttt acttgcagca ggacttttcc
     2821 gattaggaac actgacttct tttatcccag cacccgtaat tacggggttt acttcaggta
     2881 ttgctatcat tattgcatta ggtcagatcg ataatttatt tggtgttcac tcggaaggta
     2941 caaacgttat tgaaaaacta agtagttatc aaacgatagg atttccgata aacacagcct
     3001 ctttgatcat gggagggcta gtcattttag ggatgtttat ctatccaaaa aaatgggcga
     3061 aacgaatgcc tagttcgctg ctggctatca ttttggcaac agcagttatg ctattgttcc
     3121 atttaccagt agcaactgtg ggtaagattc cacaaacatt gatcagtagt aatcgtttga
     3181 atatgggaga ttttagcttt tctgccttgc aacaagtggc agttccagct attagcattg
     3241 ctttattagg aatgattgaa agcttgctat gtggtgcttc ggctggccga atggcaaata
     3301 agcccttaga tagcaatcaa gaattaatgg cgcaaggaat tggaaatctg ttgcttcctt
     3361 tctttggcgg gattcctgct actgcggcca tcgcccgtac aagcgtggcc atcaaatctg
     3421 gtgcacaaac tagattagct ggtatgatac atgcagttgt cttactgctc tccatgcttg
     3481 tttttgcgcc agtgatgtca cagattccaa tgccagcact tgcaggtgtt ttgattgtga
     3541 cggcttggcg tatgaatgaa tgggaaacga tcaaggagct atttactaat aaatattggt
     3601 cagcagttct attgtttatc cttacgatga tttgtacagt tatctttgat ttaagtattg
     3661 ctatcgtgat cggggtgatc ggcggctgtg tattttttgt tgtcaaaagc gctgctatca
     3721 cgatttcatt agaaacgatc gattggcaaa gaatggggct gccagaaacg gaaaagctga
     3781 ataactggac agtagtgtat attagtggtc cactgttttt tatgtcttca gaaaaactca
     3841 aaaataaatt ggaagaacta aatcatcacg atggtgtcat tttctccatg cgaggcgtac
     3901 caagtattga tttgacggca cgtgcagttt ttgatgaatt tcaagaaaaa gcagagcaaa
     3961 gaggacaaac gattatctat acttccttgc agccagaagt agaaaaacaa ctttctcact
     4021 tgtgggaaca tacattcacg catcaacatc aaacagttgc tcatgcgttg acagctttac
     4081 atgcgaaata tactgcaatt agccattaaa acaatgaaaa agaacagaca agactgtaaa
     4141 ctgaagattt tatggtcttg tctgttcttt ttaatattgc tgtaaaagtg aagctagtac
     4201 ttgccagtct tgatggataa caggtacaag ggagcgattg tagaaaatag ctgctggatg
     4261 aaaaagtgga agaattcgat agtttttttc tgaccattcg taggcatcct tttgttctgt
     4321 taattgtaag acaggccctg tatagagttg accatgattt ttggtaaccg tatattcttt
     4381 gccaagtaaa cgttgaagac caatgtttcc aagtgtcgca attaattttg gctggacctt
     4441 catcagttcg taatccaata ttggtgcatg cgcaaatact tcggaacgag taggtgtccg
     4501 atttggataa acagtttctg actgttgtgt ccgtttattg atccgatgag tcacgcgata
     4561 cgggcggctc cttacagcac tggtgatata cacctcttct cgtgttaagc cagttgaaga
     4621 aagtgcctgc atcaattctt ttccagcttg accgctaaaa ggaacatgcg tatcaatctc
     4681 tgttttccct ggggcttctc ccacgagcat cagtttagga tgaagaggtc cttgcccagc
     4741 gataaatcct tctacaggac ggccgttcat tcttaaagaa gcttcttgta tcagttcttt
     4801 tgggtattcc atttatgctc ctcctttttc ttttattata agaataaaag aaacacaatg
     4861 cgactatttt atttggtaga actgtgagga tagaaaagca gaagtattgc ttgaattttt
     4921 gttttacttt ttgattttaa atttagttga ctaacggttg ctgttctggt aagctagtac
     4981 tgttgtaatt gtggactcca cagctacaac cgctcagaac aagtaataag cacagcatat
     5041 gctgtcactt cggatggcga gtctaagtat atcagaagga ggtgctttat agcatgtacg
     5101 caattattaa aactggtggt aaacaagtaa aagttgaagt gggacaagca atctatgttg
     5161 aaaaacttga cgttgaagca ggagaaaaag ttgtttttga cgaagttatc ttagtaggcg
     5221 gagaatctac aaaagtaggc gctccaactg ttgcaggtgc aactgttgaa ggtactgttg
     5281 aaaaacacgg caaacaaaag aaagtcgtta ctttcaaata caaacctaaa aaacattcac
     5341 accgtaaaca aggtcatcgt caaccatata caaaagtggt tatcgacgcg atcaacgctt
     5401 aatagcgttt gagaaagaag gcctatatat gatcaaagga acatttaagc gaaatgacgc
     5461 aggtcagatc gtttcattca ctttgaccgg gcatgctgat gctggtcctt atggaagtga
     5521 tatcgtttgt gcgggtgttt ctgcgttagc gatcagcact gtcaacggga ttgcctcatt
     5581 agccggtttt gagccgattg ttgaaatgaa tgaagaagaa ggcggttatc tttataccga
     5641 agtcacttca ggaatgacac aagaacaaaa caatattgct gagattctat tagaaaatct
     5701 tttattagga ttacaatcga tagaagctga aaactcagaa tatatccaaa ttaaaacaat
     5761 taatgataaa taggaggtgc agaccatgtt gttatctatg aatctacaat tatttgccca
     5821 caaaaaaggt ggcggttcta catctaacgg ccgtgactca gaatcaaaac gtctaggtgc
     5881 taaacgtgct gatggacaaa cagttacagg tggatcaatt ctttaccgtc aacgcggtac
     5941 aaaaatctat ccaggtgtaa acgtgggtat cggtggagac gacactttat ttgcaaaagt
     6001 tgatggcgta gtacgtttcg aacgtaaagg ccgtaacaaa aaacaagtgt ctgtttatcc
     6061 agtagctcaa gaagcttaat aaataaactg ctctatctct tgtgtagcaa gggatggagc
     6121 ttttttgtaa attatgggat tttttttgac catacttttg accatacttt cttagaagtt
     6181 aatataattt gttagttttt gtgttgtttc ttcttttttg ctgttagtaa tgtgtgtata
     6241 tgtgtccata gtagttttta tatttgcgtg gcccattctt tcttggactt ctttgtgatc
     6301 tgcgcctgcc tcatatagca ttgatgcatg tgtatgtcta aaaccatgca gaccgatata
     6361 aggtaagccg gcacgctcac gatatatatt gtatctttct gccactgact gattaattgt
     6421 gaaattattt tcgtcattgg taaacaaata attagcttta gtaaatccta attgaaataa
     6481 gtactttctt tgttcgagct tccaaaattt tagttgcttt atagtttttt catctaaata
     6541 aattattcga tttgattttt tggttttagg agtttcagat atataatatt tatctgaacg
     6601 agctacagtt tttttaatgg ccatttcatt tgttttaaaa ttaatattgt cccaagtgag
     6661 tgctaatatt tcgccgattc tacatccgct gaatgctaat agtctaaaga gcgtatagtc
     6721 tctgttttta aaatatggat tctgttcttg agatacttca tttagaaata attccaactg
     6781 ttcttttgta tataatttta atttcttttc tgttttaatt tgtggctttg gaataagtaa
     6841 atttaatgtt ggattatcag atgttaaacc aatatttata gcatacttaa aaactttatt
     6901 catatagttt atgaaaagag ggtactgctt tgaagttcct ttttcactcc aatcatttac
     6961 taccttttga caatatgccg tattaatttt agaaagctta atatttccaa atttgggata
     7021 tatatgttta ttaaagatta ttttagtcct tgaatatgaa ctttctttta cagttttttt
     7081 gtagctttct agccataatt ctgctgcttc tttgaaagta gtatttgtac ttgtgggcat
     7141 tcctgttttt tgtaattcta gttctaacct agaaagtgct gtttttgctt ctttttgtgt
     7201 tctaaatcct cgtttagtag tatatttttt ctttccagtt agcggatcaa taccgagata
     7261 agttttaaag taccatgctt tttcgccatt tttcttttta tattgtttta tcattgccat
     7321 caatattacc cctattcaaa ttctaattct gccatgctat cattgaaaaa ttcgatcagt
     7381 ccttgttcct ctataaattc aagccatatt tccttcccgt taattaatgg aactcctttt
     7441 tctttacgaa ttgtttgata caagttagtc acgacttgtt tccacatttc taatacagct
     7501 ttatctattt tcttatcatc ccagcgtttt ttttggggtc tatttttgtt tttttcaatt
     7561 tgataattag tagctttgat gataaatgtt tcgtatccgg ttgtgctttt ttctaaccat
     7621 tctttgaatt ctgtatatat catttttgtt cacctttcta tttttgaacg tatgttcttt
     7681 tttgcttaaa aaattttaga gagctgcata acaatagatg cgagctttta ataattcttt
     7741 cacaagtggt tcgaagcgaa cgcttaaatt gttatcttct ataaatttca tgtaattgat
     7801 attgtgtggt tctaattcag actttgcaat atatctatct aataattttt caatcatgaa
     7861 gcgatcagcc tcatattcca tttttgaatg gagagagaat gctaagttat ataaaaaata
     7921 attatcatga tgtttagaag cgtgtcctaa ttcatgtaat aaggttcttt gtcttctata
     7981 cttagataaa ttagctttta ttactatggt attcatacaa gcaacataat ggccatcggc
     8041 atcaaggttt tcacgctctt ctaccttaac gccgagttca ttaattatca tttcaatttg
     8101 actgtccaaa tgactcacct acttatttag ttatcttttt tctctaaata tgcttcaatc
     8161 atagctgata gtatttccct atcattatcg gtgagcggtt ttccgtcgct actcataaca
     8221 gatgctaaag cttcttccac ggttagtttt ctttcctctg gtgcaactcc cgcgttagga
     8281 tcatctgtgc gtcctagcaa ataatctgtt gatacatgga agtagtcagc aactttttgc
     8341 aaagtttcta ttttaggact ttgtgtcttc catttataaa tagagttttt tccaaatcct
     8401 attctatttt ctaattcaga aatagaaata cctctttttt tacagagctc tttaaccctt
     8461 tcgaaaagca tgtcaaatca accttcctag agcgtacgat aataatttat acaa
//
LOCUS       DACCXM010000112         8483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCXM010000112 DACCXM010000000
VERSION     DACCXM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.115"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            30..329
                     /locus_tag="IVY61_002288"
     CDS             30..329
                     /locus_tag="IVY61_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7971362.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            594..1469
                     /locus_tag="IVY61_002289"
     CDS             594..1469
                     /locus_tag="IVY61_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971363.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1466..1684
                     /locus_tag="IVY61_002290"
     CDS             1466..1684
                     /locus_tag="IVY61_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971364.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1671..4298
                     /locus_tag="IVY61_002291"
     CDS             1671..4298
                     /locus_tag="IVY61_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971365.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4313..4531
                     /locus_tag="IVY61_002292"
     CDS             4313..4531
                     /locus_tag="IVY61_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971366.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            5713..6031
                     /locus_tag="IVY61_002293"
                     /pseudo
     CDS             5713..6031
                     /locus_tag="IVY61_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6050..6364
                     /locus_tag="IVY61_002294"
     CDS             6050..6364
                     /locus_tag="IVY61_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971367.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(6417..7190)
                     /locus_tag="IVY61_002295"
     CDS             complement(6417..7190)
                     /locus_tag="IVY61_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971368.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            7295..7540
                     /locus_tag="IVY61_002296"
     CDS             7295..7540
                     /locus_tag="IVY61_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971369.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            7696..>8483
                     /locus_tag="IVY61_002297"
     CDS             7696..>8483
                     /locus_tag="IVY61_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971370.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAV"
BASE COUNT         3275 a         1092 c         1577 g         2539 t
ORIGIN      
        1 gaataattaa ttaaaaagga gaggtttttg tggaagcaat aaaattaatg atttcagaag
       61 aacaggagga aatgtttcta aagcagtttt atcaaattgc tacaaaagct attgaaaagg
      121 ctcaagaaaa tgcggggatg acaagggaat atctcaatca aaaagaaatg gcggaatact
      181 taaatgtgag tgaaaacttt ttaaaaaatg ctgtgagaaa tgagggattg cccgtagttg
      241 aattaggggc aagaaaattt tattcgaaaa aggcagttaa tgagtttatg tttgctcgaa
      301 tgaaaagagg ggaaaatatt ggaagatgat gaagtagaaa ttataaaaaa atgctttaaa
      361 aaaagaaaaa aagtgttccc aaaagtttgc agactggggg aacactcaat aataaagtgc
      421 tgagaaaact ttatttatag ctattatatc aaaaaagaag gtgaaaaggt gttttcttct
      481 ggaaatgttg tagttgacaa acttagagaa ataaattttg acggaaatgt aatccacaca
      541 catggtacga tcaattaaga aaaaaaacga aaaaaggtgt tgagaagccc tatttgaatg
      601 caatagttat cttagcagaa attacctatt ggtatagacc taaaaaaatt tttaacgatg
      661 atggtcagct acttggttat caaaaaaagt ttcttgaaga tattttgcaa aaatcatata
      721 agcaattaag caaaaaaaca ggattatcag aacgagctat aaaagatgca attgtgtact
      781 tagagaaaaa ggggattatc aaaagggttt ttagaaatat ttattatgag aaaagagcag
      841 ttacattatc aaatgttatg tacattgttt taaatgtaca taaattgata gaaattactt
      901 ttcctacttt ggattgcgaa aaaaaggagt atatgccgaa aattcgtcag aggggtatcg
      961 aaaaaaagag atatgtctct ttaaaaaaag gtagaggtat gtacaaattt gtatatacgt
     1021 atacaaagac tacttcacag aacatatcaa agactactct cgttatggaa gaagaggata
     1081 ttaatataaa gaggatttac agtaagatta gtgttcttta tgacgcaaat aacgaagatg
     1141 gtcgaaaatt atttcaagtg tatcactatt tgagaaacgc taatgtagat acagaagata
     1201 tcagaaaaat cattgaatat ttatctgaaa atactcagta tcttattgct gagtgtatta
     1261 taaaacaaca tgaagcatgc ttttttcaaa gaaaaactga agaagggtta tacgattatg
     1321 taacatattt tttgaatgga cttcagaaaa gatataaaag tcaattttta gaaaatgtag
     1381 agcctataga tcagttcaaa aaagctttcg aaatcaaaga gttacctgaa attactatgc
     1441 ataattggtt gaaaggagaa gaatcatgat taaaaatcgc ttaatagatc aaatgtggtg
     1501 tcttgatcaa aatacagata ctctcgaatt aatcgagagt atctgcttca acacagtagt
     1561 tctcgattta agagctgaaa atgacaattg tgtaactcat atcataatta atcaaaaaat
     1621 ggctcagcag ctctctgaga gcctaagaaa atgggctgaa ggagaaaact atgaaagcaa
     1681 ttaaagaaaa aatcgttagt agaaaaataa actggttagc actcagtgta cttgttttaa
     1741 tgattagcgt tgtttttaca agtaatattg ttgaagctag cattttcaat tggtttgatg
     1801 acacaaaaag tcaaacagag tttttgaata attctcttta tagaccttac ctaaaaaaat
     1861 acaatggaga tttccttagt cagttcggga tttggcttgg atgggctgtg gtaaaaggca
     1921 tgtttactgt gactgattcg attcaaaata tgattccaga tgtgttggat ttgttcaatt
     1981 tcattgaaag tacagggcta aataatgttt atcaatcagt cataaataca attgtcgttg
     2041 gattaatgat cctttcattg atgtttgttg gctacaagat gattactgga aaaggaacaa
     2101 tagatttaag atctgttgga atgaatatag tgatgtctgt agcgttaatt cttttaatgc
     2161 caacaatgat ttctagcggt attcaatttg ccaaaatatt ttacaatgat gctactacga
     2221 ttacaaattc agatgacgga gtcgcttggt cattgataaa acaaggagta acagatctgg
     2281 catatattaa taaaacagat caatactcaa gcattgataa aacggaggat cgaaataagt
     2341 taacacgaaa aaattttcaa cagacagatt taacacaagt gttgacggat aaagtgattg
     2401 ataaattgga aaaagaaaat ccagctgcgg ataatcttcg ctatgaatta gttgaaaatt
     2461 ctaataatga atttgttgcc acaaagtttt ctgataactt cttatcaaca ttttctgata
     2521 gcttgaaatc aggttattat cgatatcaag caaatctttg ggggatttcg attgggttga
     2581 cagcgttggc aatcgcctat gtgttcagcg cgtttgtgat cattacagca atattagaac
     2641 ttgcatttaa acgtgtgtta ggtgttcttg tttttgcgac agatattgaa acaggacaac
     2701 gttcaaaagt tgttttatcc gatattttac aatgttactt aacagttgga tttcaagggt
     2761 ttggactatc tatgtttgct atgtttatca attttttaaa tgctggacaa ggtatctcca
     2821 caaatatttt tataaaaaca attgcttata tctgtgctgt gtttgtgctg ataaaaggca
     2881 gtggaacagt catgagatac tttggtgttg atattggact caaagaaggc tatgggcaac
     2941 ttgcttctgc ttttggtatg ggcgcaatgc tgtttagaaa aggatcaact ggatttaatc
     3001 gtgctaaagg aagcggaaat ggaaatggat cagacagtgg agaaggagaa gatcgaaaac
     3061 cagaaaagaa tttcggcgaa actctatcaa aaaaagctgg caaaactgga agagctttgg
     3121 gatatgccca tgaacgtgga ttatcaggtt tagcttcaga tggagctaca atggctagtg
     3181 aaagagcaat aaaaccattt aagagtatgc gtgatatggc taatgatacc aagaataagt
     3241 tcaaagaagg tttagatgat ggtacagtgt ccgctattaa taaaaatagc aaacctatgt
     3301 tagctaaaaa taaagaagat gaaactggaa aatatgctga ttccatgcca acacgtcttt
     3361 cggatagaaa agatggtgct aaggttgaaa atgccgatag aattatgtct agttctgaac
     3421 gtatgcgtga agcaatgaaa aataatgctg aatcgaataa taatcctgta agtgcaattc
     3481 agcaaaaagt tcaacaagat attgaagaac gtaaaaatgc tatgcacggt caagctaagt
     3541 ctgctgaaga attaattaat cagaagcgac aagaagctaa atacacgcct gaagcaatga
     3601 atcgtgagga aatgttaagg aaacgtgtag aagggcgtac aggagctaat acggacgaaa
     3661 aagaagcgtt gaccaaagaa agaatccaag aagctaaaaa ctcaaatacc ggattagaaa
     3721 aattggtcaa agaaaatcta caaaattcta caagtagtca aggtggccga tcagtagatg
     3781 tccgagaaaa tattcaaggt tcattgaatg gtcatgtcgg acggaatgtt gatgtaagag
     3841 agaatttaca aagctcttca aatggtcaag gtactaagac aattgatgtt cgagaaaatg
     3901 tacaaaattc tacaagtagt caagggttta aatcaatgga tgtaagagag aatgtacaaa
     3961 attctacaag tagtcaaggg tttaaatcaa tggatgtaag agagaatgta caaagttcta
     4021 caagtagtca aggattcaaa acagtagatg ttcgggagaa cgttcaaaaa gatagtggag
     4081 aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga cagaaagtca gcgaaaaaat
     4141 ttgattcaaa tcatgaaaca gtgattattg atagtgaaat ccgtgaaaat gataagggat
     4201 caaaaccaag acgaagattt acctatgaag acaacgagtt attcagagat acacttaatg
     4261 atcctaatcc gcttttcgat aatctattaa aaaaataaaa gggggaattg tcatgaaatt
     4321 aattctaaag attagtacgc atgatttctt agaacttgac gaattatgtg aatcagtaaa
     4381 gaacgcaaca aatgaaaaaa tcacggtcac taattatatt gaactgatga ttcaagaaga
     4441 attaaagatg aaacatgaga taaaaaacaa cttgaaatat tgttatctag aaaaaagtga
     4501 agagataatc catgaattaa atggctttta aaaattgtat ttgacatgaa tttacgacaa
     4561 ggaattagtg gggattaagc gattattctc ttccacttta gagaaataat gggttaaaaa
     4621 gccagaatac gtagagcaat attgacaacc tataaaggag ctattgttga aaattgaagt
     4681 aacaaaaaga taaagagagg tttacatcta tcagattaaa agtgatagta cacccatgaa
     4741 acctgcaaaa gagttgaacc taatgtatat cggtttaact ctttttattt gcaaattaaa
     4801 aaaatatcac cgaagtgacg aatgaaacta tgaccaaatt aagcaagaaa atatatcttg
     4861 caaataaagt agtataaaaa gatattgata aagccaatca aaactttgaa tttcaaacgt
     4921 tagataatca aacagataca aaaaatgaaa tcagttctac gcgtacgatt attaaaaata
     4981 ataaattttc actagaagaa aatggaaata aaatcaaata attaggaaga aaaaacaaag
     5041 caattagaaa agcctgcaag caaatttgag taatcgagat ccaatttttt taaggaagtt
     5101 taacaaaaat atgtaattct attatcacta ttaaagagaa agttggagcg gaatataaag
     5161 aaaagagaaa agtaattaaa cgcaaaaaaa tgtagaactt aaaaaatata aagaggaaca
     5221 accgttaaaa ttaggaatga tgttgtgtaa acattataat tgctatgata taaatgaagt
     5281 gttaaagaaa gcaaatttaa accaattttg tgaacagaaa agccttagtc actatgacca
     5341 aaactacgga cgaaactatg accaaaagta ggtactaaat tatgaccgaa aataggtcca
     5401 aaatggattt ttttatgata tcaaaaatta tgaaatgatt ttgaaagaaa accagttggc
     5461 acagccaact tagccacctt cctgtaggaa ggtgctatca gttttccctt atgctctttt
     5521 ctttcttttt tgtttcatat gtcaaatgcc attttgaaat gtaccatctt aagaaattta
     5581 tccttaaaaa caattgcgtt attggataaa aagatgctac attatcttaa aaaaggaatc
     5641 aaatatgatg aatttagtgg ctagcatatc gtgtgattaa gatactttat agtgtaagtt
     5701 gaaggagaaa aaatatttaa aatatacttc ccatttcagt gggaagtaaa attaatcttg
     5761 ttgtttctac tagtaatgta tctttttgga aactatgtaa cgatgtaaac aagatttttt
     5821 tcagaagaaa aaacgataaa agttgtgata aacgatttaa aaaataggag aaaatatatc
     5881 aagcgcatgg tatgaaacgc acatttaaaa agattcgact aactaaaaaa ataagtttac
     5941 aaagcattgt tgtgtattac aagtgatcta tacactgaaa aatgatagag aaaatctgat
     6001 acgtccgcaa caatggacaa ttgaaaatga aagtgaggaa attggaagaa tggctgatga
     6061 aaataaagtt gttccgcttg aaacactagt gataaataag gaagagtttg agcaactttt
     6121 tcttgatttt tttgttcaat atgacaggat ttatgatttt agttcgtcac tagatttatt
     6181 tgaattagag gcagaaataa tagaaaacag tcgagaggag aatggtaaaa tcatcatttc
     6241 agtagaaaaa ttcaatgaat tattctcaga aacatttgaa atagatacag attattatca
     6301 accacaagaa gttttccaat catttagaaa tcagattatt cgctcaggta agaccgtaaa
     6361 ctgaaaaaca ataaaagaaa cgttgaaaga ataattattc cttcaacgtt tcttttttag
     6421 aatgttttaa tgaccaaagc agtttcattg tcaggcttag gtaattcatg aaatcccgta
     6481 tttatagtgc tcaattgatc aactaaccat gttttacttt ttgaattgta gataaatcta
     6541 aaattaatat cgttattatc gtgttgaagg ggcgtctcca aataatctgc tgtatattca
     6601 tagtcaacgt aacctgctaa tacaaaagag agtatccctt tatttaatgt tagatttaat
     6661 ggataatcat acgactttat ttttttcaac ttcatattgt acatatcttc agccataaaa
     6721 gttgagttcg tgatttcatc ttgggtctca ttaaaaaagt tatccgaata atcgcttatt
     6781 tttgaaaagt ctttattggc accagcctca aaaaattgtt caactttttc agtataaagt
     6841 tgtgctaacg ctcctttttc aatattaact ttaatatttt catgtctatc aatttgtgtg
     6901 ctgttcagta agggaagatc agcttttagt tttatatcgt cttgatttct ttcaatttca
     6961 attggcaaaa tcttttcagt agattcagtt tctgataaag gaacatctag ttttttatca
     7021 tgataataag cttcaaatcc atctggaacc ggaaaaaaat gtattgggta attcttttct
     7081 gaggttgttg tactagtttc cgtacttgta gaacttgtgt cagaagtggt acacccgctt
     7141 atagttccga tgagtaaagc gcaagttaga atgattgcaa atttcttcaa aaaaattcct
     7201 ccttaaaatt gaatatggac taatactagt cccattgtag cataccgaat ctttaagatt
     7261 aataagaaaa aggactaatt ttagtccaaa aggtgtgcta ttcttgaaag aggaaaaaat
     7321 tattgataaa gaaaatgtaa tcggtagcaa tattagacga gtccgattag aaaaaggaat
     7381 tggtcaaacc gagctgatta gagacctaca gcttcgtaaa attgctataa ctagggaaac
     7441 gttggttaag attgaaggtg gccgccaaca cattaagttg gatcaattga aagccatcaa
     7501 agatattcta caggtgtctt atgaagattt gcttcaatga ctacttgtag tcatttcgtt
     7561 tgaaattatt atgttacacc cttatgtgtc acgaagaaat actggtataa taatgaatgg
     7621 gatatgtaac gatagggtat actgttatgt tgcctaataa attgtggatc aaaatacaag
     7681 aatggaggga tatccttgta taaggcatat gaattggatt ttacaaatgc tgacttaaat
     7741 gcattctcct caagctcgca atcatataca catgtagtaa atcaaataga tcacaaccaa
     7801 aaatctatta acaagagaat agaaaatcta tttgaaagtg aaaatgattt agttgattat
     7861 tattctaagg acagcaaaat tctagatgca gacgaaatta ttaaagattg gtttccaact
     7921 ataaaagcag atatttttat atctcactct catgctgatg aaaaattagc tatacgtttt
     7981 gcatcatggc tatttgaaaa ttttggtctt actgcattta ttgattcttc ggtttgggga
     8041 tacagttctg atttattaaa aaaaatagac caaaaatatt gctataagga acaaacaaaa
     8101 acatatgatt atgataaaag aaatgtaaca actagtcatg ttcatatgat gctttctaca
     8161 gctttaaata atatgattga tagtacagaa tgtctgtttt ttcttaatac acctaattca
     8221 attagtttga gcaatgaaat cactaatgaa cagaaattta cgtattcgcc atggctatat
     8281 agcgaactga cgacagcttc aatagtggaa aaaaagaatc cacgattgga aagtaatcct
     8341 caaatgagca ccgaggatgt tagaagcatt ataaagcact attcttcgca agaaaatctg
     8401 caaataaaat attctttaaa tttaagtgaa ttaacttatt tacgaaaagc tgatttgaaa
     8461 aaatgggctt tagatgctgt gga
//
LOCUS       DACCXM010000113         8464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCXM010000113 DACCXM010000000
VERSION     DACCXM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.116"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            624..1487
                     /locus_tag="IVY61_002298"
     CDS             624..1487
                     /locus_tag="IVY61_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7971371.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1616..3238)
                     /locus_tag="IVY61_002299"
     CDS             complement(1616..3238)
                     /locus_tag="IVY61_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7971372.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3487..4008
                     /locus_tag="IVY61_002300"
     CDS             3487..4008
                     /locus_tag="IVY61_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971373.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4194..5924)
                     /gene="spxB"
                     /locus_tag="IVY61_002301"
     CDS             complement(4194..5924)
                     /gene="spxB"
                     /locus_tag="IVY61_002301"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP7971374.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            6089..6907
                     /locus_tag="IVY61_002302"
     CDS             6089..6907
                     /locus_tag="IVY61_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP7971375.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(7010..7207)
                     /locus_tag="IVY61_002303"
     CDS             complement(7010..7207)
                     /locus_tag="IVY61_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971376.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7376..7954
                     /locus_tag="IVY61_002304"
     CDS             7376..7954
                     /locus_tag="IVY61_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7971377.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         2544 a         1524 c         1666 g         2730 t
ORIGIN      
        1 aaaccgtgtc acagtccctt tttccgaaca aacggtgttc aaaagcttgc atctacggca
       61 ctaagcccaa tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt
      121 gatgcagagc agctttttca caacctctcc actaaacggt gttctcctgc caactcctcg
      181 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ttccttgtga
      241 ctcggagcta aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc agctgaccct
      301 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttccgttt ttttctctaa
      361 tactcaggtc aaaacgctga tttcacaacc tctgattcac ggtgttccag aagtagcttc
      421 tacggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
      481 atttcttgaa gctgaccact tctgtcacaa cctcttattt tgattagaac ttgcgaaaat
      541 aaaccggtta aagtgtgtct agcgcaagag atttgctata atagacgata agggtaaaat
      601 gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac agattcttgt tgtgatctgc
      661 cataccatgt attagcaaaa gaaaaagttg attttttatc aatgaatctc caattgaatg
      721 atcaagagtt agtagatgac ctcggaaaga cattcaatta tgaccgctac atgaatcagc
      781 tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt tggccgatat ttagaatttt
      841 tccgttctta tgcggaaaaa cagattcctg ttttatatgt ggctttttcc agtggattaa
      901 gcggctctta cagtagtgcg cttcaagcgg ttgaattgct gaaggaagaa tatgaagacc
      961 ctgagatcta tgtttttgat acaaaagctg caagtattgg ggaagggctt ttagtattgg
     1021 aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga aaccctagag tggctggaaa
     1081 gtaattattt gaaagtccat tcatgggtaa cagtagacga tctgaaatat ctagaacgag
     1141 gcggtaggat ttccaaagca agtgctactc ttggcagtct attgagtgta aagcctatta
     1201 ttacggtgga taaagaagga aggctgacga atgttgacaa agttcgagga agaaacaagt
     1261 cgatccagac aatcgtcaat gagacggtaa aaacaatcgt tgcaccactt gatcaaatcg
     1321 tttatgtggc ttacgctggg gatctagaga gcgcggagaa ggcaaaacgg cttttggaag
     1381 agcagataaa gatgaaagat gtgaagcttt atcccttagg accaacgatc gccagtcaca
     1441 caggctatgg ctgtatcgcg attttttcga tgggaatcag tcgataaaag cagtaactca
     1501 ttctgtaaaa aagcccagaa attgattgta acaaaaacag cgactcttaa aagatagatg
     1561 agcatcctat ctcttagagg tcgctgtttt tttatttttt aagctaaata ggccattatg
     1621 cccataactc gttcaattgt ttttgaatat ctgcattttc caaaaattca tcatatgtcg
     1681 tatcagctcg atcgatcaca cctttatccg aaacggcaat gatgcggttt gccaatgttt
     1741 ggataaattg atggtcatgt gacgcaaata agattgagcc agtaaatgcc atcaagccgt
     1801 cattcaatgc tgtgattgat tctaagtcta aatggttcgt tggatcatct aatacaagta
     1861 cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg gactttctct ccaccagata
     1921 aaacattcac tggtttcaat acttcttcac cagaaaacaa cattcttcct aagaagcttc
     1981 ttaagaaggt gttatcatct tcttctttac ttgcatattg acgcaaccag tctaaaatcg
     2041 ttagatcagt atcgaattct ttcgttgtat cctttggtaa ataagaatta cttgttgtca
     2101 caccccaacg tactgttcca gtatctggcg taatttcacc catgataact ttgaatagcg
     2161 ttgttgttgt tatatcattt tctgcgacga aagcgacctt gtcttctttg ctcaaattaa
     2221 aagatatatc atctaaaatt ttctttccat caatcgttac acttacattt tctacctgca
     2281 ataagtcgtt tccaatctca cgttcaggtt tgaatccaac gaatggataa cgtctagaag
     2341 aaggttggat atcgtctaaa gtaatttttt caagcatttt tttacgtgaa gttgcttgtt
     2401 ttgattttga tgcattcgca ctaaagcggg caataaattc ttggagttct ttgatttgtt
     2461 cttctttttt ggcatttgcg tttgcttgaa gtttagccgc tagttgacta gattccaacc
     2521 aaaaatcata atttcctaca taaagtttga ttttaccaaa atctagatcc gccatatgtg
     2581 tacatacttt gttcaaaaag tgacggtcat gggatacaac gatcacagta ttttcaaaat
     2641 tgatcaaaaa ttcttccagc caattgatcg attggatatc cagaccgttt gttggttcgt
     2701 ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc taataacact tttacctttt
     2761 gtccaccagt taattcactc atttgctgat ggtgcaattc ttcagggatg ttcagacctt
     2821 gcagtaaaac agctgcttct ggttcagctt cccatccatt aagctcagca aattctcctt
     2881 ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc ctctttcatg taaatcgcat
     2941 ctttttcttt cagcacttca tacagacgct tgtgtcccat gatgactgtt tcaagtacag
     3001 tatagtcttc atagtcgtaa tggttctgtt tcaatacggc catccgttca tctggtccca
     3061 tagtaacgga acctgtggaa ggctgcagat cccctgataa cactttaagg aacgttgatt
     3121 tacctgcacc atttgcacca atcaagccat agcagtttcc gggggtaaat ttaatattca
     3181 catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac atcatttaca gtaatcaatt
     3241 tttttcctca cttttatcat tattagtttt gacaagtctt atagaattat ataggggaaa
     3301 ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca cgtgaaacag aatgaaaaaa
     3361 ataaaaataa aagcggagcg aaaatagcta ctataggatt tattagtttg caacagcata
     3421 atttttgacg ttatttataa gagggagtac tataaaactg taaaagaaat gaaagaagga
     3481 ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat tgattttggg aattttattt
     3541 gtacttgtct cattgttatc atttcaagac cctgtcggaa atttagttgc aattgtagta
     3601 gtctttgcga tttttgcctt tattaaaggg atatttgaac tgtttgtacg gaacagatta
     3661 aaagagctaa caggatataa aggaaagatg cccttaataa tcgggatcat cgatattcta
     3721 gttggcgtct ttttcttatt caatattggt gcaggagtcg cagcattgcc ttttgttttt
     3781 gcagtatggt ttattgctga ttcagtttta gcgttattta ctgctgactt ggcaagagga
     3841 gtaagtgaag gatattattg gtttacgatc atcgtcaata ttttaggaat tttattagga
     3901 attttcttat tgtttaatcc aatttcttct gcgttgacat taagcttttt agttggattc
     3961 tattttatgt tatttggtat cacacatatc gtctatgcat ttagataagt aaaatcaaac
     4021 aaataaaaaa gccggcttcc gattacagtt ttactgttgt aattggaagc cggctttttt
     4081 atcatggaca aatatgcacg aggtgatttt taggtgtcga gacaaattta tgcaaaaatg
     4141 tgagaagcta tatacacaaa ttttatctta atactcgaag catagcgcag ttgtcacaac
     4201 ctcttttcat ataattcatt caatactggc aaatcatgga cttcatattt ttctttgaat
     4261 gcttggattt cttcttcaga atattttctt ggatctaaat gcagatcttc tactggcaat
     4321 gggcgcttgt tattgatctt cacatcgatt actacaggac gatctgattt tgcagctgca
     4381 tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa agctatttgc cccgagcgct
     4441 tctccaactt taccgaaatc tgcatcttct aaaaatacac caaatttctt ttgttccgta
     4501 tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg aaaagacaac attaataatt
     4561 ggaagctgat atttgacttg tgtaatgata tcttgcatga ccatagcaaa cccgccatca
     4621 ccacttaagg taaatacttg tttttctggg tagcttagtt gtgcggcaat tcctcctggc
     4681 acaccattac ccatagttgc gaaccatcca gatgtcgtat gtttttgttt accgttcata
     4741 ttcaataaac gaatagaatg gatagtcgtg ttaccgacat ctgtgacgaa aatcgcatcg
     4801 tcttcagcaa tacgattgat ttctttatag acagcctctg gacgaatcgg ttcttcctct
     4861 cgatcttcaa aagatttcaa ccaatttacc catttttctt tattttcttg atttgctagg
     4921 taccagctat cagcagggcg agcatcccct aactcgacaa gtctttgcaa agctgtgttt
     4981 gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc caaattttga cgcatcgata
     5041 tcgatttgga taaattcagc agttggatta aaaaatgcac gtccaaatgg gaagtcgcta
     5101 ccgacaaata agatcagatc ggcttctgcc aatgcttcat ttccgggttt agtagcgaca
     5161 cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa taattccttt tgctagaacg
     5221 gaagcgacaa caggaactga aaaatgttct gagaattctt tgatttgcgg aaatccgttt
     5281 cgcgttcctt gaccaacata aagaactggt ttttttgctt tttcaagata aggcaaagca
     5341 gctagcaaat ctttttcttc tggtaatatc acacttgttt tgtgagtgtg agcggtggag
     5401 aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag ggatagtcac gatagccacg
     5461 cctttatgct cataggcggc tttaatggct tcatctacta catgcggaag actttgtggt
     5521 gtcatgactg tacgattgta tacgctaaca tctgcgaaaa taggattttc atttagttcc
     5581 tggaaagagt tatagttcat ggatgtagaa gcaacttgtc ctaagaacgc tactagaggc
     5641 acatggtcca tctgtgcatc atacaaacca ttgatcaaat gtgtcgcacc aggtcctgca
     5701 gagccgaaga cagcgccgac tttccccgtt aattttgcat ctgcagcggc tgcgagtgcg
     5761 ccgacttctt catgacgaac ttgaatgtag ttgatttcgg ttttttcttg atatagcgca
     5821 tccataattg aattgaaaga tcctccggga atgccgtaaa tatggtcgat accccagctt
     5881 tcaagaactt ttaccattgc gattcctgca ttgatttttg acatggtgtt tcactccttt
     5941 agcaatgatg atacataaat tttttgtatc tagagtatag aactttttat cgaaaatccc
     6001 aactaattcg tcttttcaca aaaatgtagt catgtttttg ggaatatgct agagtagtac
     6061 atagatagat tcaagagagg ggaatggcat ggttgaatgg ctattgagac ttgaaccttg
     6121 gcagcaagca cttgctggga cagcttttac ttattttatg acagctcttg gtgccgggtt
     6181 ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac ttgatgttag gatttgcttc
     6241 tggtgtcatg attgctgcaa gtttttggtc tttgttagat ccggctatta caaaagcaga
     6301 agaaaatgga gatattgctt ggctagttgt aagtattggg tttggtttag gcggtgtgtt
     6361 tttatatatg gcagataaaa cactgcccca tatgcatttt ggaccacaac atgaagcaga
     6421 aggattgcct acccatttaa aacggacgat cttgcttgtt ttttctatta cattgcataa
     6481 cattcctgaa ggtttagccg tgggggtagc attcggtgca gcagcaactg cggataatcc
     6541 gacagccgct atattagccg ctgtttcggt ggcgctaggt attggtatcc aaaacttccc
     6601 agaaggagca gctgtatcaa tcccgttgcg acaagaaggt ctgagtcgta aaaaggcctt
     6661 tgtttacgga caagcttctg gaatcgttga accaattgct ggagtgattg gagcgctact
     6721 agtaacgaaa gtggagctgc tgcttcctta tgcacttgct tttgccgcag gagcaatgat
     6781 ttatgtcgtg gtggaagaat tgatccctga agcacagcag acattgtcca gcaaacgtca
     6841 ttttgcagtt tttggcgtca tgagtggatt tatcattatg atgattttgg atgtggcatt
     6901 aggataaata aaaaaaatat atatgatata tcaaataggg ccgcgaactt atatcgcgac
     6961 cctatttttt tagtaagtta tatcataatg aagtgaatgg tatcatacat taataatagt
     7021 tttttctatg tgtatttcct ttgatccagc gaacaagcca gatgataagt gcgattggga
     7081 ttagaacttt gaccgcaaac cataaaagcc cgcttaggac actgacaaaa agactgatga
     7141 cgaagctagc tgcaattagc actaaaagaa ccatgaagat atttaagaac atattttttt
     7201 gacgcataca aaaccctcct cttgttcatt ctttactata gaaaaagtat aacagggatt
     7261 gtttaaaaac acctccaact gaagactgat ttttacttca tctacttaaa tgatcaaaca
     7321 attgcttttt ctctggttta tcatctaaaa tagagcggaa aaggaggaga atatcatgcc
     7381 taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg attatcatga ctattttagt
     7441 tttagaactt catgaaccta acggagacac gtgggaggcg ctcgctgcat taggttaccg
     7501 attttttatc tatattgtca gttttatcat tttagcgatt tattggaaca atcatcatca
     7561 tttatttcaa gttgtacata agataaatgg acgggtctta tgggcgaaca gtttctttgt
     7621 ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc agtgagtaca tcaatagcct
     7681 ggctccagaa atgacttatg gttttgttac cttgaccgct aatattgcgt attatatctt
     7741 ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa gcattgaagg cgatgttgaa
     7801 taattacaag aaatcgtatc tatcgattgg tttgaatgtg ttaggaattg tcttagggta
     7861 tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg attctagctg cttggatcat
     7921 cccaagtcgt accatcgaat cacaatatcg gtaaaatgat ccgtgtttaa aatttaagag
     7981 aaaaatatag aaaaaataaa taaacgatca aatatccgag tgatactagt attcaaagag
     8041 ggatgcagat ggatcaaaaa agaattcatg taggactgat tactagtttt gtcacgatgc
     8101 ttatttattt ggatcaaggt catttgactt ggacagtagt tggtgtcgta ggatttttga
     8161 cattattcac agcaataaaa aaataaacat ctttatgtaa tctgatgttc tatctattta
     8221 agaaaagcat gtttacagca gtatgattct gttgtaaacg tgtttttatt tgtggaaaat
     8281 ttaattttta gaaaagtgta tcgaaaaata gatagttagt ttttccacag tggataactt
     8341 cgtaagagat aaaaataaaa aattagtcaa agaagggcgt atagatttct tttgatagga
     8401 gatatacaac agaaatgaat aaatattaac gtgttttcaa acagtataaa acggtgtggt
     8461 tttt
//
LOCUS       DACCXM010000114         8451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCXM010000114 DACCXM010000000
VERSION     DACCXM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.117"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1150
                     /locus_tag="IVY61_002305"
     CDS             <1..1150
                     /locus_tag="IVY61_002305"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP7971378.1"
                     /translation="IGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAVNR
                     ANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFARS
                     FEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAMMI
                     TQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKELS
                     GRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNLYT
                     GSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQEML
                     DSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1172..1924)
                     /locus_tag="IVY61_002306"
     CDS             complement(1172..1924)
                     /locus_tag="IVY61_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7971379.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2288..4954)
                     /locus_tag="IVY61_002307"
     CDS             complement(2288..4954)
                     /locus_tag="IVY61_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7971380.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5295..6746
                     /gene="cls"
                     /locus_tag="IVY61_002308"
     CDS             5295..6746
                     /gene="cls"
                     /locus_tag="IVY61_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7971381.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7075..7890)
                     /gene="thiD"
                     /locus_tag="IVY61_002309"
     CDS             complement(7075..7890)
                     /gene="thiD"
                     /locus_tag="IVY61_002309"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP7971382.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            8184..>8451
                     /locus_tag="IVY61_002310"
     CDS             8184..>8451
                     /locus_tag="IVY61_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971383.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQ"
BASE COUNT         2507 a         1744 c         1498 g         2702 t
ORIGIN      
        1 aatagggatt gatcgtcttt ccttttttat tcctaattta tatttagata tgacagagtt
       61 ggcagaaagc cgtggggatg atcctgcaaa ataccatata gggattggtc aagaccaaat
      121 ggcagtcaat cgtgcaaatg aagacatcat tacactagga gcaaatgctg ccagcaaaat
      181 cgtaacagaa aaagaccgtg agctaatcga catggtcatc gtcggaacag aatccgggat
      241 tgatcattca aaagcgagtg cggtaattat ccaccattta ctgaaaatcc aatcttttgc
      301 tcgttctttt gaagtaaaag aggcttgcta cggtggcacc gcagctttgc acatggcgaa
      361 agaatatgtc aaaaatcatc cagaacgaaa agtactagtc atagcaagtg atattgctcg
      421 ttacagcttg gcaagcggtg gtgaagtgac gcaaggtgtc ggtgctgttg cgatgatgat
      481 cactcaaaac ccgcgtattt tatcgattga agacgacagc gtatttctga cagaagatat
      541 ctatgatttc tggcgtccag attatagcga atttcctgtt gttgatggtc ctttatctaa
      601 ttctacgtat atcgaatcat tccaaaaagt ttggaatcga cataaagaat tgtcgggtcg
      661 aggactcgaa gattatcagg cgattgcttt ccatattccg tatactaaga tgggaaaaaa
      721 ggcattgcaa agcgtattag accaaacaga cgaagaaaat caggaacgtc ttatggctcg
      781 ctatgaagaa agcatccgtt acagccgacg aatcggtaat ctttacactg gttcattata
      841 cctggggcta acttctctac tggaaaattc gaaatcacta cagccaggag atcgcatcgg
      901 tctattcagc tacggttctg gtgcagtaag tgaatttttt acaggttatc tggaagaaaa
      961 ctatcaagaa tatctcttcg cacaatctca tcaagagatg ctagattctc gaacacggat
     1021 cactgttgac gaatatgaaa cgattttcag tgagacgctt cctgaacacg gagaatgtgc
     1081 agaatatact tcagatgtac ctttttcgat tacaaagatc gaaaatgata ttcgttacta
     1141 caaaatataa taaaatcata aaaagaaccc ttcacgagtc gtgaaaggtt ctttttttat
     1201 ctgcggacca aaggagcagt ctataggtca gcttagtcgt actaaggatt tctcgttttt
     1261 cactgaatag tttgcgaatg tacctggtca caacaggatc ttttgcgtac gcgtcatagt
     1321 atagcttcat caattcttca gtaattgatt cctgtgtaaa ataagtaaaa tcttttttct
     1381 tcactttgaa tccttcatca attaagtatg tttcaaattg atccattaca actggcgatg
     1441 tattaggatc ttcttctatc attcctagtt gcttttcgat aggataaaaa acatcatcga
     1501 ttgattccac tactctgatt atcacgcacc atttgcttga ctttttcatc aatttttcta
     1561 attgaataag atgtaccaat gctggatttg ctgctgtaaa aaccatttca tatgtctcat
     1621 ttgattggat aaattcttct aacgactgtt tgataaagga aacatttggc aactcatttt
     1681 tttctgcttc tttttttgca ttttctagca ttcctgaaga aaaatcgatc aaatcatagc
     1741 gattaatttc ttttgctaag gaaagaaatc tgccagatcc tccagcaacg tctgctgctg
     1801 tttcagcagg caggactttc tccgttttca gataattttt tacatcttca gcaatagtta
     1861 cttgcgattc ttgctggatt tcataatatt cttcagcaaa ttcatcccat tctttttcat
     1921 tcatttgatt tcctctaatt ttttgttata tgttttcact tttcggaata aatcattctc
     1981 ctcttttatc tattctatct cttttgatga ataataaaat gattggcttt ccgtctatca
     2041 taaataaaaa aagggactgt gaccaaagtc gtgtcaaagt ccctctttcc gaataaacag
     2101 tgtccaatca gatgaccctc agtattagct caaaatgctg attttacaac ttcttcctta
     2161 aacggtgttc tcctgccaac tcttcggtca caagtcacaa tattcaacaa acatgagaag
     2221 ctgtttgttg aaattgcacc ttgtgactca gaattaaacg gcaggctcac aacctcgttg
     2281 attcgtttta tttatttcct tgtactcgta caatgatcaa tttagtgatt tccatcaaaa
     2341 caattgtcac aagtgcgatt cccatacatg taaagacttc tgtcaatcca aatgctgatg
     2401 ggatagagaa gacttctttc agtcctggga tcattgttaa gcagtaaagc gcgctacata
     2461 caactacagc tagtaagaca cttctgttag agaagaaccc tgcttggata gctgtttgtg
     2521 tgtttgaacg tgctgggaat gtttgcaatg tccgacacca aatcaatgtt gaaaatgaca
     2581 tggctacacc gaattcaggt gaatgctgca tgccaagcca ttgagcaaag ataacagcaa
     2641 tcccaatcag cacaccacga tacgttacag aaatcaatgt tttgtccgta aagattcctg
     2701 aatttggatc tcttggcggg cggttcatcg tagcaggttc agctttttct gtacctaatg
     2761 caattgctgg cagtgaatca ttgactaaat tgatgaacaa cagttgtaat gctgtaaatg
     2821 gattggacca gtcagcaact aaggcaaaga tgatcgcaat gatcgcacct aaatttcctg
     2881 caaatagata agaaatagct ttcttgatat tatcaaacac attacgtcct acttctactg
     2941 cgctgacaat agaagcaaag ttatcatccg tcaataccat cgctgctgca tcttttgcca
     3001 catctgtacc cgttcccata gcaattccga tgtctgcttg tttcaatgct ggtgcgtcat
     3061 ttacaccatc acctgtcata gctgagatat gacctttatt ttgccaagca cgaacgatac
     3121 gaattttatt ctcaggtgaa acacgtgcat aaacagtgac tttttctaaa acttcgttta
     3181 gttgatcttc tgtcaattca tctagttccg tcccggtcaa agccaggttt ccttcttcaa
     3241 aaatcccgat ttgttttgcg atagcaacag ctgttgtttt gtggtcacca gtaatcatga
     3301 ttgttttgat tcctgcactt cttgcttctg ttaccgcctg agtcacttca gcacgtggag
     3361 gatcgatcat agcaagcaaa ccaacaagga tcaaatcgtc ttcatcttca aatgataaat
     3421 caaaattatc aagtggacgg taagcaaaag ctagtacacg taatgcgcgt gttgaaaaag
     3481 cttcattttg ctcttggaat tttttcatca attcatccgt catcggaacg acttcaccat
     3541 ttagcaatac ttttttactg cgttggaaga cgatatctgg tccgccttta acaaacaagt
     3601 aatttttgtt atcaattgtg tgtccagtag acattaattt tcgatctgag tcaaaaggaa
     3661 gttctgcttg tcttgggtag ttgtttcgga tttcctgata aggctggttt aatttttcac
     3721 taaagtcgat aaacgctact tccgtagggt cgcccaattt agtaccgtct tcactgatcg
     3781 atgcatcatt cgctaaaaca ctgatttcga tcaagcgacg ttcgtcagct gtccattttg
     3841 aagggtcatt tgtgaaatcc ccagtatcgc cattacctaa aaagtagtca acaactgtca
     3901 ttttgttttg tgtcaatgta ccagtcttat ctgtacagat gatacttgtc gaaccaagtg
     3961 tttcaactgc tggaagcttc cgaataatgg catgacgttt tgccatttta ttcgttccca
     4021 aagataacac gattgttacg atagactgta atgcttctgg aatagcagct acggctacgg
     4081 ctacggcaaa catcactgcg ctgacgatat ctgcagtcat atctcctgta ccttctccaa
     4141 gataaatacg aaacagttgg atacctaaaa tcaataaaga caatgccaaa atagccaaac
     4201 ttagtttttt actgaattta tccagacctc gttgtaacgg agtgacttgc tcagttgttg
     4261 attccaaaag gccagcaact tgtccgatct ccgtattatt cccagttgaa gtcactaaga
     4321 atttaccgcg tccgtaagtt acgatcgttc cactgaatac catatttgta cgatctccga
     4381 ttggtaccgt acttgtgata tccgttactt ctttttctgc agaagtcgac tctcctgtca
     4441 gcataccttc atctactttt aatgatcccg catctagtaa acgtccatca gcaggtacat
     4501 aatcgcctgc gtctaacaac acgatatctc ctaccacaag ttcgtttgcc ggaatcgttt
     4561 tttccttacc atcacgaatc actcgagcat cgggcgctga aaggtttttc aacgcttcta
     4621 aagaaccttc tgcttttttt gtttggacaa cactgattat agaattcaac atcaatactg
     4681 caaaaataac taagaattcg atgtagtcac ccatgaccat ctgaatcagt gcgacaatca
     4741 gcaatacgat aaccattgca tctttgaaag tttctaagaa caatttccaa gttggcttct
     4801 tcggcttttc ttttagctgg ttcaagccat ctctttctaa tttttccttc gcttgcgtgg
     4861 tgctcaatcc atcggttgag ctctgcgttt gctgcatcaa cgagtcgatc gtttctttat
     4921 agaccgcttg ttgtgcttgt tccttgtttt ccaaatttca tcccccattt tttgatcttg
     4981 gccctagtcc tgtctgaaaa aaaagagacc taagacaaat acgcatttgt cttaggtctc
     5041 accatttcat acaataccag aaaaccattt ggttaactta ctgttgatat tatcgcaaca
     5101 aacgttgccg gttactcccc taaggagata ttcaactgta ctaatatcct accttattaa
     5161 cctacaaaaa gcaactactt tagcccgatt tacctacatt ttcatgaaag gagtgaaaag
     5221 tatattcacc ttggtaggct tctttcttct atcatgctat actaatgtta ttgtatgtaa
     5281 ggaggaacat ttttgtggta tctagtatta taaccgccct ttatctatta aatgcactta
     5341 ttgctttggt ggctattttg atcaaacccc gagatgtagc agccatttgg gcatggctct
     5401 tagtattgtt tgcccttcct ggggtgggct ttgtattata tttatttttc ggacgtggat
     5461 taacggataa gaaaaaattc tatctccgac aaagtgactt gaaagaatta gaaaactttc
     5521 agtcttttag aggagatacc attgaacatt acgatcctga catgggcgat aaagacaaac
     5581 agcaatttgt tgacttcttc tcttcattaa atcgtatgcc gctgacaaga atgaattctg
     5641 tcactcttct cacagacgga caagagaaat tggattcact gcttcaagat ctaaaaaaag
     5701 ccaaacattc gatccatatc gaatattacg catttgtgac agataatatc ggccagcaag
     5761 tcttacatgt tttagaagaa aaagccgcag aaggcgtgga agttcgaata ttatatgatg
     5821 catttggctc tcatggcaca aaagcaaaag atttcaatcg tctaatcaaa aatggtggac
     5881 atgtccatac atttgttacc tcacaaaggg cattacttcg tttccgattg aattaccatg
     5941 atcaccgaaa aatcgttgtg atcgatggaa agattagtta taccggtggt ttcaatattg
     6001 ccaatcaata tgtaaataca acaaaaaaat tcggctattg gcgcgatacg catatacgga
     6061 ttttcggtgc cgcttcttct ttgctccagc ttcgcttctt aacagactgg aacgtctcgg
     6121 tacctgaaga aaaaaaggtc ggctatcatt tgaattattt ctttaaaaaa gcagatcgag
     6181 atgaatctaa gcttgctgat acatccatcc agcttgtttc aagcggaccg aataacgaaa
     6241 gggaacaaat caagctttca tttatcaaat tgattacttc tgctaaaaaa cgtgtttgga
     6301 tacagacacc ttaccttgtt cctgatgaaa gtgtcattgc tgctttaaaa atcgcaactg
     6361 cctctggtgt agatgtgaaa atcatgattc ccaacaaacc ggatcatcct tttatttatc
     6421 gagcaacaca atattatgct cggcagctga tcaaggaaaa tgtacaaatc cttgtctatg
     6481 agaacggctt cctccatgca aaaacattga taatggatga tgaaatctgc atggtaggtt
     6541 cagcaaatca agatattcgt agctaccgat tgaattttga aacaagtgct gtcatttacg
     6601 atcctgagtt tttagaagaa cttgctactc agttcaaaga agatgagaca cattgttcat
     6661 ccatgacaac tgaaacagtc aaggaaatgt ctaactggct attattcaag caacaaattt
     6721 ctcgattatt ttctccaatc ctataatttt tttattcaag tagagaaaaa tatactaaga
     6781 ttaaagggac tgtgacaaaa atcttgtcac agtcccttct tccgaacaaa cggtgttctc
     6841 ctgccaactc ctcggtcaca agtcacaata ttcaacaaac atgagaagct gttttttgaa
     6901 cttgttcctt gtgactcgga gctggacggc aggctcacga cctctttctt aaacggtgtt
     6961 caaaagcttg catctgcggt aataagtcca accaagttaa aaacataaga agctgttttt
     7021 acttggctgt tcttattact tgatgcaggg caactttttc acaacttctt tcttttactc
     7081 taattcacca gctgcccaat gattgatggg gccatatttg tgccctacat ttatttcttc
     7141 acgaattgca cggtttacat agtcttttgc caatcggatc gcttgttcaa ctgtttttcc
     7201 tttcgctaac tcagctgcaa tacatgcaga tagcgaatcg ccagttccgt tgatccggtc
     7261 ggtaggataa aatggtgcat ttaaccaaaa tgattttcct gattcgaaca atacgtagtc
     7321 attgactgtt tcttgctgcg catttttagc atgatgtcct ttgatcataa cgttttttgc
     7381 acccatatct tgcagttctt ttgctgcttg cacaaaatct tcttcagctg ctaatgtccg
     7441 tccagtcagc ttttctgctt cataaaaatt aggtgtcagt acttcggcca aagggacaag
     7501 cttttctttc aagctttgca aagcagattc ttctagtaaa agatttccat gtttcgttac
     7561 gattacggga tccacgacta atggtccaaa atcatatttt tggtaatttt ttacaactgt
     7621 ctcaatcaac tttgaatctg ctagcatacc tgttttagct gcacgtattt gaaagtcctt
     7681 cgcaatcagt tcaaattctt tgtcgataaa atctgttggc aatgtcacac ttgctccgat
     7741 tccgtaagaa ttacctgcaa cacatgcggt catgacagaa acaccgtaaa catttcttgt
     7801 aaagaatgta tgcatatctg cttgcatccc tgcgcttcca tcgctatctg atcctgcgat
     7861 cgtcattgct tgtacatact gatcttccat caaatcgatt cctccatttt tataaaaaga
     7921 tttttcataa tactgttact gtttctatca tatgtttttc aaagtatccc tcagtaaata
     7981 taccactacc ttttcttggt aatcaaattt atttgaaaaa acatttgcaa taaatcagaa
     8041 aatagtggtc gtttatttgt attcgctatt ctaataataa ataaccacta tttgatttat
     8101 atatatttca gtatccttct tgttttcata taaccaaagg cgctatgatt tccatatgaa
     8161 aaaaataaag tcaggtgaaa aaaatgaaac ttgaggataa aaaagtcatt ttgaagaaaa
     8221 tcaaacgact accagaccaa atagatggct atacgatctg ttctttcaaa aaagacagga
     8281 gtataaaagc ttatacgcaa aacacagaaa aaggaataat ctatttccta catgaagaag
     8341 gttacgatac ccaaaccttt atgtttactg aaaaaaaaga atttcataaa cagatgaaaa
     8401 aactgattga aaaagaattt ccaagaagtc ataaactcta tgtgaatcag c
//
LOCUS       DACCXM010000115         8290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCXM010000115 DACCXM010000000
VERSION     DACCXM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.118"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1223)
                     /locus_tag="IVY61_002311"
     CDS             complement(<1..1223)
                     /locus_tag="IVY61_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP7971384.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIK"
     gene            complement(1223..2005)
                     /locus_tag="IVY61_002312"
     CDS             complement(1223..2005)
                     /locus_tag="IVY61_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971385.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            complement(2022..3779)
                     /locus_tag="IVY61_002313"
     CDS             complement(2022..3779)
                     /locus_tag="IVY61_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP7971386.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            complement(3754..6585)
                     /locus_tag="IVY61_002314"
     CDS             complement(3754..6585)
                     /locus_tag="IVY61_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7971387.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            complement(6582..7349)
                     /locus_tag="IVY61_002315"
     CDS             complement(6582..7349)
                     /locus_tag="IVY61_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7971388.1"
                     /translation="MYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGYQTLTVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            complement(7382..>8290)
                     /locus_tag="IVY61_002316"
     CDS             complement(7382..>8290)
                     /locus_tag="IVY61_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7971389.1"
                     /translation="NLWIDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFF
                     QTVNNIVQTLIKSWNSLKQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGW
                     ENLKQGTIDTFNNLVNGAQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDS
                     FLEGLQNAWKSVQDFVGGIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFND
                     VQNTVGSMADFIAELFNANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINL
                     GRQSFRLFMDDISQAMGEGADINLEF"
BASE COUNT         2198 a         1852 c         1424 g         2816 t
ORIGIN      
        1 attttgatgt cgtacaactt aaatccgcca tttattttat ccctgtcaac aaagctaata
       61 ctcatataaa atcgatctaa atttgtgatt tgataattaa cgttagctgt acctttgatt
      121 gttatttctt tccctacatc atctgttgta atatttgtac tatttgcttc caataatatc
      181 ttttctcctg gtgatgtacg atacaccaaa cgtaatttat caatagctcc tgtagttcct
      241 tcatcaaatc gaactttcgc actcagagta taggttttcc cactagcaag ctgaggtgta
      301 ttaatacgcg taaacataat aatgcttcct gtaccatcag aagtaaaatg tagcttttcg
      361 ttatccgaag ttagtgaccc gtggtaacca acgttaaaat cgctcgattt tagtttggac
      421 attaaattcg gattcccact ataatcataa ttcccgaagt cgatgctgtt actgtacatc
      481 tttttcagct tgccgaggtc gccgatttgc tgattggttt gattcatctt gtcttcaact
      541 gctttaagat tgttttgtgc cgatgtaacg ttttgagaaa cggtatcaat cttagtttga
      601 gtagcttgta gttgctctgt tacctcgtca acagtttgat caattgcttg tgtggctccc
      661 tctacaaatt catttatttg ttggtttgcc ttttcaatcg tagaatccac atttgttaat
      721 gctgcgttaa cagcatctgt tgcttcttgt ttgactccat caagtaattg ttgaaagtct
      781 gcaataaagt agtctgcttt atcttgcgct agtccgtcga ttgctgaacg cttcatacgg
      841 aaggtgaagc ctaagttatc acttgttgat ccgtctggat actcgatgta aacataagct
      901 tccaccatac cgttataagc tttcatcgct tctggcaaga tatatacgac ctgtccattc
      961 aagaagcttt cagtgatgag gtttttcgtg ataaaaggga ttggctcttt cgtgatcatt
     1021 ccatctactt catcatagat aaacatcaac aagcgcaagt tagctcctag caagtccgcc
     1081 ggtgtgccgt tctgttgttc gacgttgaac tctaacgcta tttgattatc gtatgattta
     1141 aatacaagcc ccgttgcttg caaatcgtag tctttcggct gtgtaggtac tttgatagag
     1201 ccttttttga tgacatgcgc cattatttca caccttcaat cttagttatt ttcacgttgt
     1261 tgctcattgc gggattggaa acattgccgg atgaaatatc taaagctctg ccgtttgaca
     1321 tcgttatctt tcgcgcttcg attgtcagct caaattcgat gaacgtcact cccgcgtctc
     1381 cattccataa attcgctttt gtaattcgcg cgtatctttg acgtttcact tcttctacaa
     1441 agtctccgtc accgtctgtg tagtggatac gaagcgtttg gtaacggaaa gtatcatctg
     1501 gcagattgac tgattgtcct tttcttaatt cgccttcaaa aagaacagaa gtagtgtaaa
     1561 cgcgccgcca gcctaacgag ttataaccct ctaccggttc gctgttatgt gtcattttga
     1621 tgtaaatttc gccgctataa cgagcaaaag cgacgattaa ttttctcgaa tcgtgatccg
     1681 cgaataccat catttcaaca aaactgtcat cttcaatccc gtcagggttg tctgcgcccc
     1741 atccggcttt ggttgcgtat ttgccgggcg gaatgtctaa aatgttcgga tatttttgtg
     1801 gtagcttata atcgcgtaac caacgcccac gcgcttgcat aactgtttcc ggtgtagcaa
     1861 agcccgcgtt gctttcagta gctaggacgt gcgcgtcgtg atccgagccg tcgtaatgga
     1921 tagccgcttg acgcaataac catttcacag cctcttcata actcatttct tcaaaaatat
     1981 ttggtttgct ttgaaaatct aacatttaat tgttcaccgc cttaatagtt ataagtgaag
     2041 tctctcgcgc gccctacgcc tttgcttgtc acttttcttg ttgctgtgtc aacttctatt
     2101 ttataaaacg ctaattcatc cggtgtatca acttggctag ctgtatgaga gaagccgacg
     2161 tcgcaaagaa tcatttttac tgttccaaac gccccttcga cttgttcgtg ccaatgcccg
     2221 cagaaataac ctactacaac gccggcgcct tttgtattca ttgcgaaggt ctttaatccg
     2281 ccgaacgaac cgtcattcgg ttgtcctaat ttagaccaat cgatagttac cggactacca
     2341 gatttaaacc cttctatcaa cgtgctaatc atgttttcat tgcgaacggg aaacttgctg
     2401 agacctaaag gcgtatgtcc tactaacgcc acatgatagt ttctcggaac gttcactaac
     2461 cattctccga aagcgttaat ttgtttggca ctgaaagctc ccggcgctgt atcgctgtaa
     2521 ccgtccacgt acttgtcgcc tgtgccgcct tcataaaaat cacaagtatc gaaacggtaa
     2581 attgctgcgt ttttatctgg gaacaacacg ccaccgtaca aaccgttcca atactcttcg
     2641 aagtcagcgt tgcataacat tccctttcgc ttgcgccacg cggggtcgaa acaagcgtcg
     2701 tgattccctt tgcagataat aaccggtttt tcttgtcctg ctaccgctgc gttagtgaag
     2761 cgtttgagcg tgcctaacat ggaatgtcgc gcgctccatt catcaataat gcctatatcg
     2821 ctgccgagtg aaccaagtcc gccgtcgata ttgtctccgc cgtaaatcat cacatctgtt
     2881 ttgttcccta atttttgaaa ttgagggatt gcgcgccaat gtcttaaata agaagcatct
     2941 ttgtaaccga cgccatctat acgcaagttg tgattatctg cgtgaatgtc tgtaatgaaa
     3001 gaaaagttaa atttgctgtt attaacactg ttgacaaccg tattcaaatt gcgcggcact
     3061 aggtcaacgt atttcatcgt gtcgtaatca aaaaagcgtg ttgtttctct aatttgtgag
     3121 ctgccgactg gcacttggta tttttggttt aaacgatcag ctaatgattc gtagtcgcct
     3181 ttagcttcat tcagaatgtt aatgatcata ccacctggat cgatattttc cagtatttca
     3241 cgattatctt ctaaccactg ctcccagtca tttttgccct gatccatgta atctttgaat
     3301 tttcttagca aatcctcaaa tgtccacaca tagccagagt cacgtaactg gcttctagat
     3361 attccagaaa tgactcgata ggtaaaatcc tgtgtgctaa attgttcact ccaagttcca
     3421 tcaccattaa gtgatcggaa actgaaatgt gcggtgtttt caccacccca ttgccagtca
     3481 ggctcactta aggtgtaaac aagccttgct tgcgctggac tgtattcttg tactttttgt
     3541 tcaacaggtt ggttttcgcc aaattttgtt gtattaataa aaaacggcac taggccctcg
     3601 aatgttttta gtttgccatg ttccaccact tcaacaacga acttttgcgt taaaacatcc
     3661 ccttgccgaa ttcgaaccaa atttattccg ttgtttggtt cggtggtgga taggaccatt
     3721 ttatgctgcg tttctgccat gactatccct cctttagaaa tcgatgtagt ctcttgcatt
     3781 gtggaaatga cctgaagaag atggataaaa ctcatccata aattgaaaat gaagatgttc
     3841 tccagtggat ggtcctgttg tccccatcag tccaatttgc tgaccagcag ttaccttctg
     3901 accttttgag acgtctactc ggctttgatg cgcgtatcct gtatacattc catcagcgtg
     3961 tttaatcact gtccaatttc cataccagtc aaagtaattt gcatcacctg caacaatcac
     4021 ttcgccatcc gctgaagcaa aaataggtgt attaggattt ccatttacaa ggtcaatacc
     4081 gttatgaaat tcttgtgctc ctgtgattgg agaagtgcgc cagccaaatt cgctcgtcac
     4141 tgtgattgga tccgcaatcg gcttcatata tccttttgat acaggaattt ccaaatcttt
     4201 gaacttgtca taccattctt gtgaccatgc cgtccgttct ggatgtggat cacgtggacg
     4261 ttcaaagtta gccacgaatg cttgtgctgc tgtgttgata tcggtcagat tcatgaattg
     4321 tgtccatgtg taaggataag cgctagttgc gatccattga ccatttggtg catgccacat
     4381 caacaattta aactgtgccg tgattgtgtc tggatcatca ctgatgccag cctttgtcat
     4441 taaattgatc atgtagacac gtccgctagt tgcgccagaa gaatcggtcc attgccatac
     4501 accataaccg aaccctggtg cgccattgcc ctcatcagcg gttggattag catctgattc
     4561 tccttgtgca tttccaagta aggctgcagc agcttgttgt gtaaaaccgg cacctattgc
     4621 cgttgcccaa atttgccaat atcgtttatc ccgatcagta gttacttctg gtgggtattg
     4681 tccgttccaa ccgttatcgt tccctccgga attgcctcct ccattagtgt caatttttgt
     4741 accgttaaca tagagttctt ttacatctag ccgtccatct atggttatat ttccttctga
     4801 aaaattgccg tcaccataaa gattatattt gcgcttatca gcagtaacat ctgctggaat
     4861 ttgaaaaata ggattccctc gatcgccacc gtccccagca ttaatggaaa aaatatagtt
     4921 tggctctttc cagacagcaa agccgttaat ccctttagaa ccataagtag atacaattga
     4981 gcccaagcgt tctccatgta catcatctag tccagtagac ttgacttgtt tttcaaaaga
     5041 aagttctccc ccctctgcca ctaattggaa atctttatcg tcaaatgttt ttaaagctac
     5101 cccttgcacg aggataccag acagaatgcc tgcgcgaata aaattagcat tgaaagttcc
     5161 atccaacgtc caagcggtcg tgctatcacc attgtgtaca tcttgaattg tttgccactc
     5221 acctttttta cattgtttga aagatatccc tgagttattt tggaccataa aaaagcgtga
     5281 tctaggaatg ttaggtccat ccatataaac agtttcatag atttctctac tatcactaac
     5341 accagcttca attccattta cccaataaat agaaccgcca ttatctcctg cacctcgcat
     5401 aatgtcatct tgatattttc caatctctgt cgattcgtaa aatgtcattt tgctagattc
     5461 taaactatta atattattga caatagaagc cgtttgtttt ctaacatctt gtgttaaatt
     5521 atcccctagt tcgatattcg tttgaccggt aagccgattg aatgtagttt tataaatacg
     5581 agttttatag tgataacctt tatcgtatct gtgaatagtc actgtatttc ctatcacatc
     5641 tcctccagtg acttcagctt tgaattgtac taacggtcta gcagaatcga ttaaggttga
     5701 ataagtattt ttaagtaaat ctgttggatc atctatatca tcaaacacta ctacggtttc
     5761 tcgttttctc attgatccat ctttttgtgg tatcccatac ttttgagttg attccggatc
     5821 ttcaagccaa ttttggcctt taggcttatc taaaggatca ccattcgact ttttccattc
     5881 aacatcagtg aattcaattc ttctaccgta accgtcacca acctcttcgc ctcgcccacg
     5941 acctatcatt gaagttgaga ttgagcttct atctatctct cttacaactg ttaatgcttt
     6001 actaccatat acgaaacgtg tattcgattc ttcgccaatt tgttcatata cttcgatcca
     6061 tttatccttt attccatcag aattcaaaga acacctaaag acaaattcca tacctaaggt
     6121 ttgcaattct ttcaacgctt cttttacaga gacatagtaa aaagttgcag ttactgttgg
     6181 taacattgct tctacgtgac caacgcgcca atttccttca gtaaattcaa tcaatcgatc
     6241 aagaacattt tttaagggct gcccactcgg cctaatatct ttgatgatgt aagcatctaa
     6301 ttcatttgtc gcaaatccta accctgtaaa ctctaatgtt tcaaatgggt cgctaacttt
     6361 agtaattcga tacaacgaaa aagacgactc gttttcacga atcgccatat atcttgcatc
     6421 ctctatttct ttatcatatt ttgtcgtaac gtaaagagta tctttcatta gatcactctt
     6481 atcagaacta atttcttttt cttgggagac ttcaatcaaa cttcttgtat tttttctttt
     6541 aataagtttt tgcaagtgat caaagaaata aactgtctca ctcaaattgt cgccccccta
     6601 tagaatattt taagcttccc attattgcta gttatcttct gaccttgctt aagataaaag
     6661 ttctcaaaat cactttctaa atcaatcata gaagtacaat cttctccgtt tacagttacc
     6721 tgctcatcgg aaaaatcaaa aaccaacacg tctcctgttt ttattgccgc gtcagttatc
     6781 gtgatatttt gttctccgtt tgtaattttg attgaattat tcatggataa agtaacttca
     6841 atttttcttg gtgttatagg aaactgtatc ggatttccaa tatagccatc actaacacac
     6901 tctttcgtat actttagtgg atccgcacag aatacattaa aactcgaaat aatcgagttg
     6961 gagtctcctg gaacagtatc agttgatgta tagcgaccgt agtaataata atctaattca
     7021 tcatgaaacc taatttccac gtcttcattc cggtataaat aattcaacag ttctttgaat
     7081 ttaaactgta gtttttctgg atctctatct tccagcttat atgttatttt tagtgttctt
     7141 gaaggtattt tctgatttgt aatgattgaa ccaatttgta tctcttgctg ttcaacttct
     7201 acagaaagca tttctctacc ttccaccgta agtgtttgat aaccatcaat caaatcttct
     7261 aaatacattc catcgtacat catggcagac gtcggaagga atcgtttaga actagtgaga
     7321 ttaatggttg tatctttgaa tgagtacatt ttattttctc gttgatccaa aatattccct
     7381 cctaaaattc cagattaata tctgcacctt cgcccatagc ttgtgaaata tcgtccataa
     7441 acaatctaaa cgattgtctt ccaagattga ttttaaatac agctggttta gtagagccac
     7501 ccatatttat tttatgttca acttgtgcac caatgttttt atttgcattt ttcaaatttg
     7561 cagctatatc tacatcagaa tttgcattga aaagttccgc gataaagtct gccatacttc
     7621 caacagtatt ctgtacgtca ttgaatcctc ctgtcagacc tttattcaga ccgttcataa
     7681 tagcctgacc agctggaatc aatagctttc tatcgtattg gataggtcct ttgtgttcac
     7741 gaatccaatc accaatacct ccaacaaaat cttgcacaga tttccatgca ttttgtaacc
     7801 cttctagaaa actatccatg atagcttttc cggctgctag taaatcgata tttttcaagt
     7861 tatcaaacca gccagttact ctatcaaccg tgtcactaac agcatttact aaattatccc
     7921 atgcctcttg agcaccattt actaaattgt tgaaagtatc tatagtacct tgttttaggt
     7981 tttcccatcc ctgaatgatg ttatctttgg ttccagtaat taaatcacca atccaagatt
     8041 tgaatgaagt ccaaatgtct ttagcacctt gaactgtatt gttgaataaa tctatcgttc
     8101 cttgctttaa gctgttccaa ctttttataa gagtttgcac gatattatta accgtttgga
     8161 aaaaccattg ttttagatta ttccaaagtt caattgcacc atattttgta tcaatccaag
     8221 tttgaattat ggagaatttt agatttatcc acatttgaat tgcactgtat ttaatatcaa
     8281 tccataaatt
//
LOCUS       DACCXM010000116         8028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCXM010000116 DACCXM010000000
VERSION     DACCXM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.119"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            95..1075
                     /locus_tag="IVY61_002317"
     CDS             95..1075
                     /locus_tag="IVY61_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7971390.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            1197..2615
                     /locus_tag="IVY61_002318"
     CDS             1197..2615
                     /locus_tag="IVY61_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP7971391.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            2686..4155
                     /locus_tag="IVY61_002319"
     CDS             2686..4155
                     /locus_tag="IVY61_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7971392.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            4165..4632
                     /locus_tag="IVY61_002320"
     CDS             4165..4632
                     /locus_tag="IVY61_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7971393.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            4668..5540
                     /locus_tag="IVY61_002321"
     CDS             4668..5540
                     /locus_tag="IVY61_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7971394.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            5621..>8028
                     /locus_tag="IVY61_002322"
     CDS             5621..>8028
                     /locus_tag="IVY61_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7971395.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGA"
BASE COUNT         2781 a         1094 c         1616 g         2537 t
ORIGIN      
        1 acttatccga aaaggggaat gagagagtca aggttcttgg aatgtcgaat agtcgagtag
       61 gtagattttc tatctacaac cttgttgttt aaaaatgaaa aaaattggaa ttagagaagt
      121 tgcgaaagag gcaaatgttt ctcctacgac tgtgtcgagg gtattgaatg atcgtggata
      181 tttgagtgag gaaacaaaaa gaaaagtttt tgatgccatg aaaaaattag aatactatcc
      241 taatgaactg gcgagagcac tttttaaaaa cgaaacatat accgtaggtt tgatttttcc
      301 tacaattact aatccatttc acgctgagtt aattcaagat atcgagttcg agctttccag
      361 caggggatat aaagtgttgc tgtgcaatag cttaaacaat ccaacaaaag aaaaagagta
      421 cttgacgatg ttgagaaaga atcaggttga tggaattata gtaggaacgc acaataaaga
      481 tattgcggat tatgatattc cagggctacc tattgtagcg attgatagaa gtcttggtga
      541 aaatacagtt acagtatctt gtgataatta caggggtggg gaattagcta ctcaactact
      601 tgtaaatcat ggctgtcaga atatactgtg tattcgggga gatagtaaga taaagctacc
      661 cgcgaatagt aggactttag catataaaga tgttttgaat aaattcatgc tggaaccgtt
      721 agtcctagaa gtaccatttg taaaagaaat caatgaaaaa aatgaaataa ttaagacata
      781 tctggaaact catccaaata ttgacggggt ttttgcagga gatgatttat tggcttcttt
      841 ggcgattaac aacttaggaa gttcgggcaa aagagttcca aaagaggtta aagtgattgg
      901 atttgatgga gctcaacaaa cattgattta taatccagaa ttatcaacta ttcaacagcc
      961 tatagatcaa atatctaaag tagcagtaaa aaaattattg aatatgatta agggcgaaaa
     1021 ggagacggat gaactttcac ttccagtaaa gctcataaaa aaaaatacag cataagttaa
     1081 atcggcgaaa aattccttcg tcgatttaaa aatattttta tgaaacgggt tgacatatga
     1141 aacgggttga catatattag ttgtgaagat aaaaaccttt tcactggagg aagaaaatgg
     1201 agaaattatt aattaaaagc aataatcatc aactttacta ccaacctaag gatgtttggg
     1261 tcggagatgt tatgccttac gggaaagaag gaaaattctt tatttatcat caacgtgata
     1321 ctagaaatcc agttcctttt ggagaaccgt ttggctgggc tttagcaact acgaaagatt
     1381 ttgttcattt tgaagacttt ggggaatctc ttttaagagg ggatgacaat gagagagacc
     1441 agtttatcta cgctggttct gtatttgagg caaatggaaa ggttcacgca ttttatacag
     1501 ggtataatcg tgaattctta gcgcagggaa agacatctca agtgttgctt catgctgaga
     1561 gtgatgattt tgttcattgg gaaaaatcag aaacagcatt ggagttagaa ccacaagaag
     1621 ggtatgatat tcgaaactgg agagatccat ttgtcgtttg ggatgatgaa aaagaagaat
     1681 atttgctaat tttaggggcg cgaaagggtc aggataaaca tctacaaacc ggacgtttag
     1741 tggaatttac atcaaaagat ttaactaatt gggaattcaa aggtgatttt tgggctccgg
     1801 gattgtatac catgtttgaa atgccagaac ttttcaagat tggtgactgg tggtatcttg
     1861 tctattcaga atactcagat ggaaataaaa tactctatcg aatgagtcaa agtttggaag
     1921 gtccttggat taagcctaaa gatgaagctt ttgatggacg ggcttattat gcaggtagaa
     1981 cagcttatga tggaaaacgt cgagtcctat ttggatgggt accaacaaaa gatcaagaaa
     2041 atgataaaaa caattattta tggggaggta ctttggttcc tcatgaagtc tatcaaaaag
     2101 aggatttttc acttggggta aaacccatag acgaagtgtg gaatgcattc ataaatttac
     2161 atgatattgc cgatgtagag atagaaacag cagacacaag agaagaagta acgcttgtca
     2221 aaaatagtgg aactttgttt aatttagaaa caacaatttc atttgatgag aatcttagtg
     2281 acttttcact gagattatat aaaaaccttg agacagatga atcttacgaa tatcgcttca
     2341 atttagatga ggggcgtctt acatttgata aaaacccttg ttacccttgg tttcaatata
     2401 tggataaagg acttattaga cctatatctt taaagcctaa ccaatcttat aaactaaaag
     2461 tgattgttga tgacaatata atgacacttt atattgatgg aatcgctctt aacgctcgtg
     2521 tacatggttg ttttggtcaa gcaattacta ttacagcaac aaatggatca atgactgcaa
     2581 taaataccaa gttttcaaat gaaataaaga aataataaac gtcaatcatg tggcaggtct
     2641 gaaaatcaaa catgacctgt cacatttaaa agggaggata aatgaatgga ctacaaaaaa
     2701 attgccgaag atgtattgga aaacgttgga ggaaaagaga atatctcaag cgctacacac
     2761 tgcattactc gtttacgttt aattcttaaa gacaaaagca aagcgaatat ggaaatgctt
     2821 gaggaaatag aaggtgcaaa aggtgttctt ttcaatagtg gacagttaca agtaattttc
     2881 ggaacaggta taattgaaaa ggtttacaaa gagttcgttg aattgacagg agctaaggaa
     2941 gtatccgttt ctgaaatgaa agacgaagga gtcaataatt caggaaatag acttcaacga
     3001 gcttttaaag ttttctcgga tatctttatt ccgatcattc cagcttttat tggggcagca
     3061 atggttttag gaattcgttc gttgattacc actcctggaa tgtttggaat ggatggctca
     3121 ttcgctgatc acaatgtatt cctaaatgat ttatccaaat ttctgggtat tattgcaacc
     3181 acatttgatt atttaccagt tttagttatg tattctgcaa caaaacgttt tggcggaaac
     3241 cctattttag gattacttgt aggatttgtt atgattcatc caggactagc caatcggaat
     3301 gatttcgtca tgggaactat aaagcctgat tattggcatt tatttggatt aagcattcca
     3361 gcagttgcgt ttcaaggggg tgtttttcca gcaattttga cttcttggtt tttagcaaaa
     3421 gttgagaaaa ttacaaataa atattctcct caagtacttg cttacatttt agtcccaact
     3481 gtgacaattg ttcttgccaa ctttgcacta tttttagtgt ttggtccatt ggggaatttt
     3541 atcggtaatg ttttaggtgg aatcattgat ttcctatata tgaagatggg tgcctttggt
     3601 gcttttgttt ttgcaggcgc gttacaacca ttagttgtaa caggaacaca acatgctatt
     3661 caaggtattg aggcaaactt agtcgcaaca actggattca attatatcca acctatttgg
     3721 tcagtttcaa ttattgccca aggtggcggt tgtattggta tgtatttact ctttaagaaa
     3781 aaatctaaag atagagatat tgcgatttca agctttattc caacactggt aggtattact
     3841 gaaccggcta tctttgctgt taatttgaaa tattcaatcg ttccttttgt ttgctcgttt
     3901 attggtgctg gttttggtgg agcatttatg aaaatttttg atgtaaaagc cattggacaa
     3961 ggattgactg tattgccagg tctgactatt gctaatccag ttcttcctta tatatttggt
     4021 aatctcatag cattcatttt accaatcatt tttattctcg cttatgataa atataaagtg
     4081 tttatccctg aagctaaaca ccgtaaattt gcagaaaaaa ctgactcaaa aaaattgcca
     4141 gttcaagtaa agtgagatga gagcatgtta aattttttta gaaaaaatga aataagcgtt
     4201 gtagcacctg caaccggtgt ttttgaaaaa ctggagaaat taagtgatcc agtcttttca
     4261 aaaggaatga tgggacaagg atttgcaatt gatgcaaaaa acgaaacgat tgtatctcca
     4321 atcgatggtt ttgtttcaag tatttttcct acaaaacatg ccttaggaat aaaaacaaaa
     4381 tcaggaattg agatacttgt tcatgtagga atagatacag ttgagctaaa tggtgaggga
     4441 tttgatatta aagttcaaga aggacaaaaa gttaaggctg gggatattct tatgaccatt
     4501 aattttgtag tttttgaaaa gaatcacaag actaaagatg tcattgttgt ttttccagac
     4561 taccaagacg aaattttcat tccgaaactt gaagaagtaa aacaaggaca agaattgttt
     4621 gttcttggct aatgataagt aattaataat agaatcgagg aaaatgtatg gtttttgggg
     4681 ctattgaagc tggtggaaca aaatttgttt gtgccattgg agataaagat ttgaacatat
     4741 ttgaaaaagt cagctttccc acgactacac ccaaagaaac aatgaattta gttttagatt
     4801 tttttaatca tcataaagat gatttagaag gtatcggagt gggatcgttc ggtcctattg
     4861 atattcattg tgaatctaaa acgtatggct tcattaaatc aacgccaaaa cttgcttggc
     4921 aggattttga ttttattgga agtttaaaaa agcaacttaa tattccaact gcttggacga
     4981 ctgatgtaaa tgcctcttgc tatggtgaat atgtttctgg aaatggcaga ggcctttcaa
     5041 gtgttgttta ctataccgta ggaacgggca taggaggtgg cgctatccaa aacggaaact
     5101 tcattgaagg ttttagtcat ccagaaatgg gacatatgct tgtaaaacga cacaataaag
     5161 acaacttttc tgggacatgt ccattccacg gagactgttt agagggctta gctgctggtc
     5221 cggcaatcga aagaagatca gggaaaaagg gacaagaagt gactgaaaat gacgttactt
     5281 gggaaataga agcttattac attgctcagt gtgcatataa tacaacattg atgttatcac
     5341 ctgatgtcat tatatttggt ggcggagtaa tgaaacaaaa acacttaaaa aagaaagtac
     5401 aagataagtt tattgagttg atcaacggat atgtcgctat tcccgatgtt gacaattata
     5461 ttatgacacc aaaattgtta gataattctg gtattgtagg ttgtttagct ttagcaagag
     5521 acgttaaaga aaaatcttaa tttggaaaaa agatcgtctc gactatttgt ttggttgatg
     5581 cgatcttttt ttcaattagt aattgaaggg acgcctaaaa gtgaaaaaaa aaatgaaaga
     5641 tgaagtatta gagtatatca aaaataattt aaagtattac gatttttctg ctcaagatat
     5701 tgcaatgaaa ttttgtatta aaagaaatgt cgcttcccac tatttgaatc agttattttc
     5761 agatggaaaa ttactaaaga atgattctgt tagaccagta atgttcaagt ataatcaaca
     5821 aaagccaaaa gactgctttt ctaagtttat tggagcagac atatctttga aatccactat
     5881 agataaatgc aaagctactg taatgtatcc ccctaatggc ctcccattaa taataaaagg
     5941 aaacagtggc gttggaaaaa gttttttagc aagtttgatc tatcagtatg ctcttgatag
     6001 aaaagtaatt cataatgacg caaaatttgt tgttgtgaat tgtgcagatt atgcaaataa
     6061 tccagagcta ttgtcagcag ttttatttgg atataaaaaa ggagccttta ctggggctga
     6121 aaaagatact gcagggatgc tatcttctgc taatggagga tacctttttt tagatgaggt
     6181 tcacaatcta agtgcagaaa accaagagaa attgtttctt cttatggatt ctcaaaagta
     6241 tcgtaaactt ggtgaaagtg tgcaatggga gtatgcaaat gttcgcctta tcttagccac
     6301 aacagaagat aaaaatacct ctttgttagc aacttttaga agacgcatcc cagctgaaat
     6361 cactctgcca gattacaata atagaagtac atctgaaaaa attcaattat tatttgaatt
     6421 tcttaaagat gaagcaatta cgattgattc aaaaatattt tgttcgattg aactttttac
     6481 tgatctttta tcgaaaacat ttgaaggaaa tgttggagaa ttgaggaatg aaatcaagct
     6541 tctttgtgca gaaggttatt taggaaataa gagaaagtct tcaatttatt taggggacaa
     6601 actagacagc ggtttttgga tagatccaga aattagtata gatttaaaaa aaatgtttct
     6661 taaatcactt tctgaaattg atttgattga actttttcaa aatcacattt caattgaaga
     6721 aagcatgagt caattaagag atagaatact taaagagtgt gcaggttctt ctgagtataa
     6781 ttatttagaa agtgacgaat ttatagcatt aagaaactat gttgttaata aaatatcacc
     6841 aattattgat tcgacaggtc tatgtttatt agatgaattc ttggcggata tcagtctatt
     6901 tataatgttt attgatgtaa ttgaagaaac atcaagaaat tttttgctgt ctaagttcag
     6961 aaaatttcgt atgaaaaatg ataaacaaaa attattggca aatgagattt ggagtagttt
     7021 agaagtcgaa gaaagtaaac aagaactttt gttgaaatgg atactttttc ttgtaaagaa
     7081 gttctatgtg aaaataccag aaacccattg tctgatagtt atgcacggta aaataacagc
     7141 ttctgcgatc gcaagggaaa caaataagtt gctgaataca tatgtttatg aggctttcga
     7201 tatgccaatt gaaggtgaga ctagtgactt aattaaccta atcaacaatt tttgtaaaag
     7261 tatagatacg acgaatggac tcattctgct agttgatatg ggatcattgg aacaaatgta
     7321 tgaaaaaatt gaatccaatg taataggtga tttggtaatt ttaaacaatg tttctacagc
     7381 tttagcgatt gaatgtggga ttcaagtttg ccaaaaaaag ccaattagtc atttttacca
     7441 gatggatttt gattcgtttc aagttaaagt tcagtattat aaaggattat cacaaaagaa
     7501 aaatgtaata gtatcttgtc tctctggtga ggggatttct gaaaaagtta aggatatttt
     7561 aaaacggtat ctcgataatc aagtggagat tttgattttt gattttaacg cattaaagaa
     7621 aatagcaaag gaaaaagata ctatgatttt taagaataca atttgtgttc tttcaaccac
     7681 tgaattttac attcaaggaa tcgattgttt aaatttagaa aatctaatta atgggaatca
     7741 aaccttagaa aaattaaata agtatatgga ctcggatagc tgtgagttct gcttaaatga
     7801 attagttaaa ctctttacta ttgaaggcgc tagtactaag cttcgctttt tagattccaa
     7861 aaaagtattc aatgagatag aaaaagtact atatttgtat gaaaaatatt acgatgtcaa
     7921 aatcccaagt tttctgagaa ttaatctatt tctacatctt tctggaatga ttgaacgaat
     7981 tatgattggt gatggaataa caaatgaaca tttgaaagag ggagcgca
//
LOCUS       DACCXM010000117         8013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCXM010000117 DACCXM010000000
VERSION     DACCXM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.120"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..839)
                     /locus_tag="IVY61_002323"
     CDS             complement(<1..839)
                     /locus_tag="IVY61_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7971396.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFM"
     gene            complement(1063..3003)
                     /gene="thrS"
                     /locus_tag="IVY61_002324"
     CDS             complement(1063..3003)
                     /gene="thrS"
                     /locus_tag="IVY61_002324"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP7971397.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3340..3519)
                     /locus_tag="IVY61_002325"
     CDS             complement(3340..3519)
                     /locus_tag="IVY61_002325"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP7971398.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3566..5389)
                     /gene="cadA"
                     /locus_tag="IVY61_002326"
     CDS             complement(3566..5389)
                     /gene="cadA"
                     /locus_tag="IVY61_002326"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7971399.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5667..7946
                     /locus_tag="IVY61_002327"
     CDS             5667..7946
                     /locus_tag="IVY61_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7971400.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2346 a         1707 c         1451 g         2509 t
ORIGIN      
        1 tccataaaat attcttggat tgcttctcgt gtttttttca ctttttcgct aagcttttcg
       61 tttttagcca tatagaaata aaaaatgata tcgatcgtag caaattgggc caaaagtgaa
      121 ttagttgccg cacttcgatt cgtgatttct tttggtctag atgtttgtac gagtacatcg
      181 gctatcttgc taagcgggtt atttccaaat tgcgtcaacg caatgatttc gatattcatg
      241 cttttcgcca gctcggctaa tgcgactaca tctgcacttc tacctgagtt tgaaacaagc
      301 caaagcactt tctttttctc attgctgaca agttgcggga gcaagacgtg gatatctttt
      361 tcaaaaatga ttggcttacc gacacgagac catttttgca gaatatcttc tactactaaa
      421 gaagaggcgc caaccccata taaaaaaaca gtctctgccg attccaaaca ctctacgact
      481 ttttggatcg tttcttcttc tagaatatct actgtttctt ttatcgctac ttgtgcattg
      541 ttaccaagtt tgtttttgat agagtaaaag gactcatttg cttctatatc actaaaacct
      601 tcatcgattc cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt cagactttga
      661 aaaccgattt ttttggaaaa acgtacaact gtcggcgcac tcaacccgga agcttctgct
      721 aattcattag ctgtcattgt agggaccttc tcaatattac cgataatgta gttggccaac
      781 tttttttctg ctgaagaata atcttccatc atactttcaa tacgactaat tacgctcata
      841 agttcctcca cccttttaat gatagtctat gtaagtatac catatcagac ccttattcac
      901 ggtcgtatac tggataaaaa acaaaaaaac aatccgccca acagaatatc ttatcccatc
      961 aatcccacag attctattga gcatctgtgt ctttgaggaa tacctgttat ctattgttcg
     1021 gattgctttc tatctactac ttttttacag gaaatttacc tattagtttt ctctgctgta
     1081 atttttgact tctgcttcca tagcatcaat aaagatattc aaatcttcta cagctgtttc
     1141 tttacttcca taacgacgta cgttcactgt tgcatcttcc aattccttgt ctccaacaac
     1201 taattggtaa gggatctttt gtgtttgaga ggcacgtatc ttatagccca ttttttcatt
     1261 acgatcatca acttctacac gcaacccctt catttgcaaa cgttctttga tttcataagc
     1321 ataatcgcca tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag gtgccaacca
     1381 tgttggaaaa gcgcctttgt atacttctgt taaataagcg acaaaacgtt ccattgtaga
     1441 aacaattccg cggtggataa ctactggacg atgcgtattt tccccgtctt ctccaacata
     1501 tgtcaaatca aagcgctctg gcaataagaa gtctaattgt atagttgata atgtttcttc
     1561 tgtaccaaga gctgttttga cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc
     1621 ttctgcttca aagtaatcta gttctaattc atccatagcg gctttcagca ttgcttgggc
     1681 tttttcccac attgcgtcat catcaaaata tttatctgta ttgtttggat cgcgatagct
     1741 caagcggaaa cgataatctg taatattgaa gtcagcatat actgccacca tcaattcaag
     1801 cgtacgttta aattcatctt tgatttgatc aggacgaaca aatgtatgac catcattcaa
     1861 cgtcatttct cgtacacgtt gtaaacctga caaagcacct gatttttcat agcgatgcat
     1921 cattcctaat tcagcgatac gaattggtaa ttcacgataa gaatggatcg tgtttttgta
     1981 gaccatcatg tgatgagggc agttcattgg gcgaagaaca agcatttcgc catctcccat
     2041 atccattggc gggaacatgt cttcttgata atgatcccag tgtccagatg ttttgtacaa
     2101 ctcaacatct cccattattg gtgtatatac atgttggtaa cctagactaa cttctttgtc
     2161 tacgatataa cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc agaatggcaa
     2221 accagaacct acttctgggg aaaccatgaa caagtcaagt tcttttccta gtttacgatg
     2281 gtctctttct tttgcttctt cacggagacg gatatattct ttcaaagctt tcttgtcaaa
     2341 aaaggctgtc ccgtatacac gttgcatcat ttgattgttt gagtttccac gccaataagc
     2401 acctgccact gataataatt taaatacttg tatacggcca gtggatggaa catgtggtcc
     2461 acgacacaaa tcgatgaatt cgccttgttg ataagcagta atcacttctt cttctggcaa
     2521 ttcactaatc agttctactt tgtacggatc acttgcaaaa atttctaatg cttcctgttt
     2581 tgaaagaaca cggcgttcga tcggcagatt ttctttgacg attttcatca tttctgcttc
     2641 gattgccggc aaatcttctg cagttacttg gttttgtcca ttatctgtat cgtagtaaaa
     2701 ccctgaatcg atagctggtc caacgccaaa gtggatattt ggatagaggc gacgcatggc
     2761 ttgggccatc aaatgagctg ctgaatgacg gactaagcca agtgcatctt catggtctgg
     2821 tgtgatgatc tcaatacttg catcttcctc gattggacga gtcaaatcaa cgagttttcc
     2881 gttcacttta ccagctaaag ctttttttgc taagcttttt gaaatgcttt cagcaattgc
     2941 caatgttgtt gttccagact caaattcttt gactgcacca tctggaaaag taatttttat
     3001 cataatagtt tcctccttga aatgttttga ttgatcagat catcaagctg aaaaacaaaa
     3061 aaatcctagc atctcttgaa aagagatact aggacgaaaa gttcgtggtt ccacctaatg
     3121 ttcaaacgca aatgcgttcc tcaagcgttg taacggtccg accgcccttg cttcaacaag
     3181 ggtttcgaaa gtggtcatcc tttggttttc tcctggaaaa gtttcagcct agcttttcct
     3241 ctcttatcga agactctcaa attcagttgg ctttctcatc aatcaattat gtgtttattt
     3301 aaccactaat aaatagaaaa atcaagtggg aaactaaact tattttttcc attccccatt
     3361 tacaccgtat gttccttttt tcatctcatt gatgatgaca tggatgtgtt cttttggtgc
     3421 ctgcgtgttt ttatgaacag cttccgtgat atctttcatc atattttcta attgttccgg
     3481 actgcgacct tctactaatt cgacatgtac gaatggcata atgctctctc ctctttctga
     3541 tcgttctttt ttcaactcga ttctatcata ctatgtttgt cttgggtact gaatcaggaa
     3601 aaatggctgg atcttttttt gctctcaagg cactcaaaat agagaccgta tccacgactt
     3661 cttgcaaaac agctccgaat aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa
     3721 aaatacaaat aaagatgcca atcagtacag actgtttcgc tattctcatc gtatctcttg
     3781 aaatcactac tgcttttgct acacgcaaga gatcatcttt caagatgacc gcatctgcac
     3841 tttcactagc agctgtagaa ccatgagccc ccattgcaat accgatatct gcagcagcaa
     3901 gtgctggtgc gtcattcacg ccgtctccta ccatcgcaac aggtctttga tctttaggga
     3961 tactttctaa aatggtcagt ttatcttgag gcaagctacg ggcatgtaca gaatctaatt
     4021 ggactttttc tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc atcatcaaat
     4081 ttttcacatc aagttttctt agctccttga ttgtctttgc tgcttctggg cgaatctgat
     4141 ccgaaaatag gatcgttcct gcaaaagatc gatcaattgt tacatagatt gctgtattgg
     4201 acgtttctat ttcttcagct gaaacaaaag aggcacgtcc aatctttact tcatgattat
     4261 tgactactgc ttggattcct tgaccggtca cttctttcag ttgactaaca ggatacagtt
     4321 ggctgttttt cacgttgttc actaacgaac gagctaagat atggctagat tcctgttctg
     4381 cactagctgc atacagaagt agctcttctt tttttattcc attgactggc tgaatcgcct
     4441 ctacttccaa tatccctttg gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac
     4501 gtgcgagctt ttcaatcgtt gttccagttt ttacaacgat cccgttttta cttgaacggc
     4561 tcattcctgc aactaaagca atgggcgcag ctagaatcag tggacaaggt gatgccacta
     4621 ccagtacctc agcaaatcgt aaagggtctt ttgagaacca ccaagaaata ccaccgatca
     4681 aataagcaat caacgtgaaa gggactgcat aacgatcagc taaacggaca aatcgggcag
     4741 gcttttcttt tgattcttcg actagtttaa taatcatttg atactggctg tctgcggcac
     4801 gtttttcagc gatcatttgg atcgatccgt ctccatttaa agaccctgac ataatctgat
     4861 ctccaggttt cttggtttcc gggcgggact cacctgtcaa agaagattca tcgaccgaag
     4921 attctccttt ggtcactcga ccatctgcgg gtacaagttc gccaggcttg actagtaact
     4981 ggtcatcaat atttatttca tcgattacta tatcctgcaa ttttccttct ttgaatcgat
     5041 gggcaattct tggggtgtta tctaacaaag actgcagttc tttacccgct cgtttattcg
     5101 cgtaatcctc tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt
     5161 attctttgac ggctaatgtt gcaaggattg cggtaatcgc taatacatct acgccatatc
     5221 ttcctgaacg taacgtcttg atcatttcca aaaacatcaa gaaggcgaga atccctccag
     5281 taaatgcgac aatcgcaaat gcaagggccg gctggtgaaa aacaaattca cttaataaag
     5341 ctatcccacc aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt gttttttctc
     5401 ctcttttatg tgacttagag aggttagtaa taaatttcca gctgcctctt tcatcttaag
     5461 catagtcctt gactagcact ttttaaaaca ataggcctta attgaaaaca actatcacta
     5521 tcagcgtttc caaaattcat cagttctaca aacaaaaata ttcagcaagg attacttttt
     5581 cgattcatca gttaaaaagt accttttttc gaaaaactat gctatacttt taattcgtta
     5641 tttacggcgg aaggagcaaa catgcgatga atgagaaaca actagaacaa acgcacgtgg
     5701 atgaaacgat cgaactaatc cagatcgaac aaaatatgct ggatacaaaa caacaagaat
     5761 tgactcagga aatgaatcat tcgacaaaag acacagctaa tcacaagatt cgagctggtt
     5821 ccaatgaagc tttttatgaa tctgctgttg aatatcgcca acatgagcag gaattgcttt
     5881 tgaaatacca tactcttgaa gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag
     5941 atagtcctta ttttgcccgt atcgacttta aagaagaccg tgaaacagaa acattatatc
     6001 taggtatcgc ttctttaaga gatctggcag aagaaacaat cgttattgac tggcgcgcac
     6061 caattgccaa tctttattat gaaggtgaga ttggtcccgc attttatgaa actgaaacag
     6121 atagaataga tgtcgatctg cagttgaaac gacaatttaa aatccaagaa ggacgtattt
     6181 tatctatggt agatacgtcg gaagtaatca atgatgactt tttgctagag atattggatg
     6241 aatcatcaag caaccatatg aaaaacatcg tctctacgat acaaaaagca caaaatgcaa
     6301 tcattcggga cacaacaagt aaagtaatgc tgattgaagg aatcgccgga agtggaaaga
     6361 catccgcttt gcttcaacgt attgcttttc tgttgtatca taatcgcaag tggctcgagg
     6421 ctgaaaacgt tcttctattt tcacccaacc atttgttttc tgattacatc tcgactgtat
     6481 tgccttcatt aggtgaaagc ggcgtgccta cacagacatt caaaaattat ttggtgcagc
     6541 tgctgccaga gttttcttta ctagatgaac agcaacaaga aagtggtttt ttgctagggg
     6601 aaaaagatcc gatacaagtg atgaaaagcg gattgacctt agtcgatcag attgatcgct
     6661 atatccaagg catcacaaac tacggtccct tgttccgtga tatgaaaata aatggccgta
     6721 cgatcctatc aaaggaatcg attcgccaat ggtataaaga aacaaatgaa caattacctt
     6781 tacatcatag actttctcta ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa
     6841 aagacgaaac acgtcagcaa tgggtcaaag atctggcgga agaacagctc caagaactat
     6901 acgcaacaga tccaaatctt gaatatacag aacaaaagga gcgttcatta cgtaaaaaac
     6961 tggccaacca aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc atgaattatc
     7021 aatttgtcaa tataacgaaa caatatctcc actttctaca aactgtgcca ctttctcttc
     7081 taacacgaca taaaatcagc gagaccaggt ggcaggaaca tttgacaact gttcgtacac
     7141 acttgcgtca aagagaattg cgtcaagagg atgctgtact attcttttta ctgatgcgcc
     7201 gaacatatcc agtgacggtt acacacaaag ctcgctatat ctttattgat gaaatgcagg
     7261 atttcgcacc atcacaagtg gcattgctgc aagccttgtt cccaaaagca aatttgactt
     7321 tctgtggaga tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga tcattggaag
     7381 accttttcgt ggaccgtgaa gtcactcgtt tccaattaac gacaagctat cgctccacaa
     7441 aagaaatcac tgattttgct aatcatttta ttgtgaaaga acatcaagta gaaacaacag
     7501 caagaaaagg agaccttcct ttgctcatcc aaggaacaac agatgatatc aaattgaatt
     7561 ggttagcaga taccattcga acgactcagg gaaaagcccg gtattggcgt acagcaatca
     7621 tagctaaaac acaagaagaa tgcgaagctc tttatgctca actgccagac gagttgaaac
     7681 aacaagtcca gctgatttta aatgagagtg actttatgaa gcgttccatt attttacttc
     7741 cagccttttt agcaaaaggt ctggagtttg accgagtttt cttgtgggat gtagatgcag
     7801 aacatttttc ttctcagcaa gacaaattga tcctttacac catgtgtaca cgtgcgatgc
     7861 atgaattgac cctttttacc tccaataatg aaagtccttt gttaaacaat atggatattt
     7921 catcttatga gaaaaaagta ctgtaagtaa aaagacaact gctatcatta atgttagcag
     7981 ttgtcttttt atttacaata ataattgcag aaa
//
LOCUS       DACCXM010000118         7846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCXM010000118 DACCXM010000000
VERSION     DACCXM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.122"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            19..720
                     /locus_tag="IVY61_002328"
     CDS             19..720
                     /locus_tag="IVY61_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7971401.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1329..2345)
                     /gene="tsaD"
                     /locus_tag="IVY61_002329"
     CDS             complement(1329..2345)
                     /gene="tsaD"
                     /locus_tag="IVY61_002329"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP7971402.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2342..2806)
                     /gene="rimI"
                     /locus_tag="IVY61_002330"
     CDS             complement(2342..2806)
                     /gene="rimI"
                     /locus_tag="IVY61_002330"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7971403.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2813..3355)
                     /gene="rimI"
                     /locus_tag="IVY61_002331"
     CDS             complement(2813..3355)
                     /gene="rimI"
                     /locus_tag="IVY61_002331"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7971404.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3339..4163)
                     /gene="tsaB"
                     /locus_tag="IVY61_002332"
     CDS             complement(3339..4163)
                     /gene="tsaB"
                     /locus_tag="IVY61_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP7971405.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4252..5232)
                     /locus_tag="IVY61_002333"
     CDS             complement(4252..5232)
                     /locus_tag="IVY61_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7971406.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5256..6740)
                     /gene="galT"
                     /locus_tag="IVY61_002334"
     CDS             complement(5256..6740)
                     /gene="galT"
                     /locus_tag="IVY61_002334"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP7971407.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6752..7741)
                     /gene="galE"
                     /locus_tag="IVY61_002335"
     CDS             complement(6752..7741)
                     /gene="galE"
                     /locus_tag="IVY61_002335"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP7971408.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2194 a         1694 c         1393 g         2565 t
ORIGIN      
        1 taaggagagg actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga
       61 tatcaacctg attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact
      121 gttcttcgtt cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg
      181 ttgtatatct tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat
      241 tactactatg actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa
      301 gagacctaca cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt
      361 tcctactatg aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa
      421 atttctaaag tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc
      481 tttgaccggg taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa
      541 gatggtcata tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc
      601 acgacacgtg aaactgctgg acaagtaatc aaacaactac gtgattccgg gctgttagat
      661 tatgacaaaa agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa
      721 cgcgaacaaa aataagcagt gaccctgata aatatcaggg tcactgctta ttttcctccc
      781 taaaacttct gaaagatccg gcagttttag atcactggac aagactcaaa gattttctat
      841 gtgtgtttca gttatgtaat attcactgtt tgcaaaaaat actgttagat taactagatg
      901 aagcgtcttg tcctcatccg atctagatta ttcgatattc ctcgacaata aacgattact
      961 cagtgagggc aatcagagga acggataatg ataaataagc tgtaaaaaat agcaaaataa
     1021 caataatgat taaaagtatg taaaaaatag ggctttgaca aaaacgtgtc acagccctat
     1081 ttcgattaaa cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg ttcataaaca
     1141 tgaggagctg ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa
     1201 ctcttgatta aacggcgttc aaaagcttgc atacgcggta ataagaacag ccaggtcaaa
     1261 aacatgagaa gccgtttttc ctgggctgtt cttattactt gatgcagggc agctttttca
     1321 caacctcttt attcagataa aaccaagctt ggatcagcat tcaactgata gctagcaaaa
     1381 tttctttttt gcatttccac atgtgctgct gctccaatca ttgcagcatt atctccacaa
     1441 agtcgaagag ggggaatcac tagttctacc tcagggagtt tagctgccaa agcatcctgc
     1501 aatccttcac gcaacccttg attggccgct acacctccag ctataatcag ttgcttgact
     1561 ggataatttt gacaagctcg aagtgtttta gtaatcaata catcgatcac acttgcttga
     1621 aagctagctg ctaaatcatt tttgtctaga tcttctccac gctgttgtgc attatgcacc
     1681 aaattgataa atgcactttt taatccgcta aaactgaaat cataattatc ttcatggatc
     1741 atcgctcgcg gaaaatggta attatctttt ccctgatgag ctagctgatc gatttctttt
     1801 ccgcttgggt aagaaagacc taatactctt ccaactttgt cataagcttc tcctgctgcg
     1861 tcatctcttg tctccccaat gatttcatat gatccatctt cttgcatata gactaattct
     1921 gtatgtcctc cgctaacaag aagtgccatc aatggaaact ggaatggttt aaccagacgg
     1981 gctgcataga tatgccctgc catatggttt acaggaataa gcggcaattg gtgtgcccaa
     2041 gcaaaagcct tagctgcaga aataccaatc agcaacgagc cgacaagtcc tggtccataa
     2101 gtgacagcga ctgccgataa atcttctgcc gacacaccag cttctaccag tgcatcttcc
     2161 aagcataagg tgatttgttc tacgtgatgg cggctagcta cttcaggtac tacgccacca
     2221 aaacgtttat gactgtttat ttgagaagca acgatgttag ataagatctc tgttccattt
     2281 ctaacgaccg ctacactcgt ttcatcacaa cttgattcaa tcgccagaat caattcttca
     2341 ttcatttcat cgtctccgtt tttaactttc tgctcataat gatcgcatct tccgtcggat
     2401 tgcgataata tgatttccgt ttacctaata tacgaaactt ttctgattca taaagttttc
     2461 ttgctgcttc attagataga cgtacttcta aaaaaacatg cagcgaatct tttatttgtt
     2521 ccgtaatcag taattgtaac aaatgccgtg catgcccttt tccttgttct gaaactgcaa
     2581 cagcaatatt tgtgatctcg acttcatcta ttactttcga gtatccaata aagccacaga
     2641 tctggttatc ttcagctaaa agcagataat cagagtgagg ctgttggatg tctgccaaaa
     2701 actgcatttt cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc atccatacgc
     2761 attctgccaa gtacgcttca tcaaaatctt tttttctgca aatcaactaa tctcactcat
     2821 tttctaaaag ttttatcata tccaacgcat cttcttttgt ttcagtgtaa tatctttttt
     2881 tgatcgttgt tgcttgaaac cccaatttcc gataaagtct ttgtgcatct tgattactca
     2941 tacgcacttc taatgacatc gtctcacagc gattcattat agcaaagttc tcgatttcgt
     3001 caataagcag cgaaccgatt cgtttacgct gatatgccgg tcgaacggca atattcgtga
     3061 tatggcaatc taaaccgaac gctcttgaac cgataaaacc aatcattctt ccattatcaa
     3121 agatacctag atatagatga ggaagcggtg acttcaattc tgatataaag gcactttttg
     3181 tccatggcaa ttcgccatta tagacttctc gttcgacatc cagcagctct ttgatatctt
     3241 ctgtagtcaa ttctcttagt acgtaacgac gatcgatttg ttcgactgtt ttttctgtat
     3301 aaatcatttt atcttgaaaa aagcttctgg catacgctct aaattttttc aacataactt
     3361 tcgtctccag gtgtgtgcgt ctccaaccat ttttcttctg cttctactcg ttttaaatag
     3421 cgaggaagaa aagcttgttt ctctgcaact ggttcaacta aagcacctaa ttgagccaca
     3481 gttacaccac ttggaatatc ccatgctgga actgtattaa tcatcgcact aggcaatatt
     3541 tcctttattt cagagaagaa tttgtgacaa tcactcccga cgaaataaat agcttcattc
     3601 agcttttgta gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc aagtacagtt
     3661 tctaatctat tttctttcca gcggtatgcc ccagcgtata cattttttct tcgagcatca
     3721 aacatgggca caataatatt atccacaccg atgcaattag ccgcgatggt tttcagacta
     3781 gatactccta ctagctcttt atctaatgta taagcaatcg tttttgctgt cgttacacct
     3841 atacgaagcc ctgtataaga accgggacca tcagaaaccg cgattcggtc aatgtctttt
     3901 ggacttattt gaagatctgc aaacaattgg tcgattgcag gcatcagtgt tacactgtgg
     3961 ttgcgtttga tatttgtttg tatttgtcca agaatttgtt cgtcttctac cacaccgatc
     4021 gccaaagttt ggtttgcagt atctattcct aatgtaatca tatatttgat cctttctgct
     4081 atgacattct cgccttccat tgtagcatac ttttttattc tgaggatgaa aataccaaag
     4141 gcaaaactag tacaataata catgattatc tgctggcggg caaattttgc cccttcctga
     4201 aacacaaaaa agctgcggta tcactccatt ttcccacagc ttttccatct tttatttcgt
     4261 tgattctcga ataatcaact ctgtttccac aataatcttt cttgaaaccg gtgctttgga
     4321 attacttaat tgaagaatcg tctccactgc tagctctccc atccattcgg tataaacttt
     4381 tacagtagaa agcgagggcg ttacatattt cgctacactg acatcattga agcccatcac
     4441 agaaatatct tctggtacca ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc
     4501 taatgcatca ttagctgcaa aaagcgcatc aggaacatgc ggattcgttt ttaaatattg
     4561 cttcactgct tgatatcctg actcgacaga aaaatctgct tcgataattg gaccttcgtc
     4621 gtagcctttt tctgccaaaa cttgttgaaa tgccataatt ctgggatctt ttagccgctg
     4681 atggtttttc ttcgtatatt ctgttccagc aagcatagcg atatgttgcg ctttatggga
     4741 gacaaagtgt tcaatgactt tttgaacact tgacttaaag tcgacaacta gagagtcgat
     4801 tccttgagaa gtgccgtcag aatctaccaa aagcaaggca ttttgggatc ctttcatttc
     4861 taataactga gcttcgtcga attttcctag tgcgatcgtt ccatctgccg gaagatcact
     4921 aatggaatcc cacgtttctt tcaataaagt aacgtttgct tcttctgcct ttttttcgat
     4981 tcctaggcga atagataaat aataaagatc ttcaagctct tccgtatcgt cgtaccactg
     5041 gatcaggcga agcgtttgct tagtatttgc tgcattttta cgatgtttcg tataatttaa
     5101 gcgttctgct gcttcaaaaa ttttttgctt tgtggctaag ccaacggaaa gatcaggatc
     5161 atagttgaga actcttgaaa cagtagcagg agaaactcct gccaactgcg caatgtcttt
     5221 aattgttgcc atttgtctct cctccttttt tacgtttata aaatcgcagt aaatcgttca
     5281 aacgctgcct gtcctttttc atctcgcttg aatactccag cgtcttccag tacacgtgca
     5341 aaaacctgac ctacagattt ttgtacgaca tcggtgaccg tatctttcgt caaatcagga
     5401 tgttcttttt ttaattgttc tgcccacgga cgatgatatt cagccatctg gttttcttct
     5461 cctaataaga aatgctctac ttctactagt tctggtttca aacgaggtgg taaaacagca
     5521 agccccatta cttcgatcaa cccaatgttc tcctttttga tatgttggac atctttatgt
     5581 ggatgaaaaa tcccatcagg atgttcttct gaaacattgt tgtctcgaag gacaaggtcc
     5641 aactcaaagt aattgtccct tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca
     5701 tctgctgaaa aagctctgat agatactgtt tcatccgaat atgctttcca tttttccaat
     5761 actgagttag ctgcttcaat aagcaagttt ttctctgttg attgcaaacg gatcacagac
     5821 atcggccatt tcacgactcc tgcgatgact tgcggataat cttctagttt gataagtttt
     5881 tctatcggtg cttttgccat aggaaattca tgacgtcctg cctgataatg atcatgagaa
     5941 agaatcgatc ctccaacaat tggtaagtca gcattcgaac caacaaagta atgaggcaag
     6001 acttcagtaa tccgaagcag tctagcaaat gtttcctttg tgattttcat cgggcggtgt
     6061 tgttctgata aaatgatcga atgttcattg taataagcat aaggggaata ttggaatccc
     6121 cagctttcac cgtctagatt cattcgaata atccggtgat tggtgcgggc tggatagttc
     6181 aagcgacctc tatatccttc gttttccatg caaagcatgc aggtaggata gtctactttt
     6241 accgcctcgc gttctgctgc gatttgtttt ggatcttttt ctggcttaga taaattgatt
     6301 gtgatttcca gttcaccata ctccgactct gccggaaatt tgatgttttt tgcgatggct
     6361 cttgttttga tgtagtcatt tttcttactt aaattaaaga aatactcagt tgcttcctct
     6421 gggcttttcg aatagtgctg tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga
     6481 aaatccatta attgcgcttc aaaaatctct tgttctgctg gcaattctga aagaacacca
     6541 ttttctcttg ccttagcgac aagctgatcc aataagtcta cagattcttc cttaactggt
     6601 cttacttcaa tgctttctag agcatcttct cctaccatcc ctaaaagacg attctgtaaa
     6661 tataagcggt ccatttccat ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa
     6721 atcgtttgac tgattgacat atctcattcc cctattcttc ataaccattt ggatggctga
     6781 catgccaatc ccatgctgtt ttgataatcg ctttgatatc tgtgtattca ggtttccagc
     6841 ccagaatctc tctcgctttt tcacttgaag cgactaatcg gctaggatct ccagctcttc
     6901 ttggtgctat ttctgctgga atttcttttc ctgtcacttc tctagctgct tctagcattt
     6961 ccttgactga gtatcctttg ctgctgccta aattgaagaa gttgctttca tttccttctt
     7021 tcaagtattc caaagctaaa atatgtgcag caatcaaatc ttctacttga acataatctc
     7081 tgatacatgt tccatctggt gtatcataat cgtctccata aacagccaag gcttttcttt
     7141 gaccaagagc tacttgtaaa atgattggga caagatgcgt ttctggtgta tgatcttctc
     7201 cgatagatgc atctgctttt gctccggcta cattgaagta gcgcaaagcg acatagcgca
     7261 tgccatatgc ttggtcacac cacttcatca ttttttccat catcaattta ctttcaccat
     7321 acggattttt aggattagct ggtgtatctt ctgtgattgg agactctttc ggctctccat
     7381 aagttgctgc agtagaagag aatacgattt tattcacgtt gaactcgtgc atgacttcaa
     7441 gtaagatttg cataccgtat acgttgttgt tgaaatacat caatggtttt tcgacagatt
     7501 ctcctactaa agagcttgca gcaaaatgga ttactccttc aatcggttcc ttttcaaata
     7561 cggaacgtag aaattcttta tcccggatgt ctccttcata aaaatgagca tctggatgta
     7621 ctgcttgttt gtgacctgtg agcaaattat ctaccacagc tacttgatac cccttttgaa
     7681 ctagctggtc tacagcgtgc gaaccgatat aaccggctcc gcctagtact aaaattgtca
     7741 taaaaaacac tcctttgatt agtaaaattg tgtttgttga ttctatataa tttaaaaaac
     7801 gaagtttact tttatggtga aaacaaacaa agtaaaattt cgtaaa
//
LOCUS       DACCXM010000119         7744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCXM010000119 DACCXM010000000
VERSION     DACCXM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.123"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..107
                     /locus_tag="IVY61_002336"
     CDS             <1..107
                     /locus_tag="IVY61_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAP7971409.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="IVY61_002337"
     CDS             complement(176..904)
                     /locus_tag="IVY61_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7971410.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="IVY61_002338"
     CDS             complement(917..2197)
                     /locus_tag="IVY61_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7971411.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="IVY61_002339"
     CDS             complement(2338..2940)
                     /locus_tag="IVY61_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP7971412.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="IVY61_002340"
     CDS             3185..3391
                     /locus_tag="IVY61_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971413.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="IVY61_002341"
     CDS             3703..4422
                     /locus_tag="IVY61_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7971414.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="IVY61_002342"
     CDS             complement(4503..5186)
                     /locus_tag="IVY61_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP7971415.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="IVY61_002343"
     CDS             5405..7591
                     /locus_tag="IVY61_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7971416.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2780 a         1302 c         1464 g         2198 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
     7741 gaat
//
LOCUS       DACCXM010000120         7685 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCXM010000120 DACCXM010000000
VERSION     DACCXM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.124"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..177
                     /locus_tag="IVY61_002344"
     CDS             <1..177
                     /locus_tag="IVY61_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971417.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
     gene            190..693
                     /locus_tag="IVY61_002345"
     CDS             190..693
                     /locus_tag="IVY61_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7971418.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            753..1142
                     /locus_tag="IVY61_002346"
     CDS             753..1142
                     /locus_tag="IVY61_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7971419.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1129..3576
                     /locus_tag="IVY61_002347"
     CDS             1129..3576
                     /locus_tag="IVY61_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7971420.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            3581..5704
                     /locus_tag="IVY61_002348"
     CDS             3581..5704
                     /locus_tag="IVY61_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7971421.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            5701..6705
                     /locus_tag="IVY61_002349"
     CDS             5701..6705
                     /locus_tag="IVY61_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7971422.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            6724..7635
                     /locus_tag="IVY61_002350"
     CDS             6724..7635
                     /locus_tag="IVY61_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7971423.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
BASE COUNT         2443 a         1272 c         1738 g         2232 t
ORIGIN      
        1 caatccttag tcttactggc aattgtggtc attggtttgt ttttaggatt taagcgggag
       61 ttttccaaac tgattggctt tttggtgatt gctttgattg cggtcggatt ggtctttaat
      121 gcttctgggg tgaaggatgt cttactcagt ttgtttaacc gaattgtcgg tgcgtaaggg
      181 ggaagcacga tggaacagat gcgtgtttat attgcgaacc tcgggaaata taacgaaggt
      241 gaattggtgg gggcgtggtt taccccacct gtcgattttg atgaagtgaa agaacgaatt
      301 gggttaaatg acgaatacga ggaatatgca attcatgatt atgaacttcc ctttgagatt
      361 gatgagtata cgccaattga ggaaattaat cggttgtgtg gcttagcgga ggaacttgaa
      421 ggaacgccag ttggtgaagt tgcttctgag attcaacatg ctttcttcaa ttcttttgag
      481 gagatggtag agcatgtgga tgatattatc tattatccag attgcgatga tatgagtgat
      541 gtagcatatt tcttgattgt agaagctggt gattttggag aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtaga gacttggaga ttggaggcaa ttatttggta
      661 acgagtcgag gtgtttttga gtatccatat taacaaaatt attgaagggt agttgatttc
      721 taaactatcc ttctttttaa aggaatgatt tgatgaagaa aataaaaagc tacaccagta
      781 tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt tcaactgcct tttcctgtga
      841 cttttaacca gatggcttgg tttgttttat ccttgctgtt tgtcatcgta tttgcccatg
      901 tgcctccact atctatgata gagggggcct ttttaaaata cttaggtata ccggtggcag
      961 ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa accattggga tttttacgat
     1021 cttttattag ctatcactta cgatttaagg tcactttcgc aggaaagaaa gtcaaagaac
     1081 agaagaaacg atgggacgaa cccattactc tggtaaggag tgtgaactat gtacccgatt
     1141 aagtatattg aaaataatct tgtctttaat caagagggag aatgttttgc ctactatgag
     1201 ttaatacctt acaattactc ctttttgtca cctgaacaga aatatcaagt ccacgataac
     1261 tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc atgccttaca aattgccaca
     1321 gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg aaattaccgg gcgattaaca
     1381 gaagtagcga aacaacgaat tgatttacag acagaggcat tagtatccat gattggcgat
     1441 agtcagattg attatcgctt ttttattggc ttcaaactga ttgcgacaga tgaagaggtc
     1501 aatctaaaga atctcaagaa atctttcttc tccggctttc aagaatttgt ctatggagtg
     1561 aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg ccgatatacc
     1621 aagcttgaaa aactaatgga aagtaaattg gctcgaagat ttaaagtcag acgagtcaca
     1681 ccgagtgatc tgatgtattt gattgaacat atctatggcg aaaagggtac accatttgaa
     1741 gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg aaactttggt gaaacgttac
     1801 gacttattac gtcccagtcg ttgtttgatt gaagaaaagc cccggtactt atgcatggaa
     1861 catgagaatc atgaatcata tgtagcgtac ttaacaatta atacaattgt gggggaaatg
     1921 gagtttccct cctcagaact tttctattac cagcaacaac aatttacatt cccaattgac
     1981 actagtatga atgtggagat tgtgacgaac aaaaaggcac ttgctaccgt tcgcaataag
     2041 aaaaaggaat taaaagactt ggacaaccat gcctatcaat ctgacaatga aacaaattcc
     2101 aatgtcttag atgcattgga ttccgtagat gaattggaaa ctaccttgga tcagtccaaa
     2161 gaatccatgt acaaactgag ctatgtggtt cgagtaagtg ccgaatcagt agatgaacta
     2221 aagcgccggt gtgatgaagt gctagatttt tatgatgata ccaatgtaaa actagtccgg
     2281 ccttttggcg atatgatggg gctacatgaa gaattcttac cttctagtaa gcgatacatg
     2341 aatgattata tccagtatgt tacgtctgat tttctcgccg gacttggttt tggcgccact
     2401 cagatgttag gggagttgga aggcatttac tttggttata acgtagatac ggggcgcaat
     2461 gtttacctga aaccggcatt ggcctcacaa ggggtcaagg attcagttac caatgcgcta
     2521 gcagcggcat tcctcggttc actcggtggc ggaaagtcct ttagtaacaa tctcttagtt
     2581 tattacgccg tcctttttgg tggacaagct gtgattgtcg atccaaaagg ggaacggggt
     2641 ggctggaaag aaaccttgcc agaaattgcg gatgaaatca atattctcaa cttaactagt
     2701 aagccaaaaa atcaaggatt acttgatcct tatgtgatta tggagaaaaa gaaggattct
     2761 gaaagtctag caattgatat tctgaccttt ttaacaggaa tttctagtcg agatggggag
     2821 aagtttccag tgttgcgtaa ggccattcgg acggttaccc aaagcaatca acggggatta
     2881 ttacaagtaa ttaatgagct tcgaaaagaa ggctcaccag ttgcggaaaa tatcgcggat
     2941 cacatcgagt cctttaccga ttatgatttt gcccatttgc ttttctcaga tggttcgatt
     3001 acccagtcga ttagtttaga taggcagtta aatatcattc aagtagcgga tttggtttta
     3061 ccagatgcag aaaccacttt tgaagaatat accaccatgg aattgcttag tgtttccatg
     3121 ctaatcgtca tttccacttt tgccttggac tttatccata gtaatcgaga aatcttcaaa
     3181 attgtagact tagatgaggc gtggagtttt cttcaagtag cgcaagggaa aacattatcg
     3241 aataagctcg ttcgtgctgg gcgggctatg aatgccgggg tttactttgt cacgcaaaat
     3301 agtgatgatt tactggatga acgcttgaag aataatatcg ggttgaagtt tgcgtttcgc
     3361 agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggcttgga tcgagaagat
     3421 gaaagcaatc aaaaacgcct acgagatttg gaaaatggcc aatgcttgat gcaggatttg
     3481 tatggtcggg taggcgtggt tcaggtacat ccagtctttg aagaactctt ccatgccttt
     3541 gatacccgtc cacctgttca agagagaatg gagtgagggg atgaaaaaga aactttttcg
     3601 gattgtggga atcgttttta ttagcatact cactctgata gttctattga gtttagttgg
     3661 tacggtagcc gaggcgacag ggctagttga tgatacagtt gaagcaggaa atctatattc
     3721 caaatattca ctgaacaact atcaactaga tttctttgtg gatagctctt gggattggtt
     3781 gccttggaat tggggtgatg gcttagggaa aagcgtgatg tatggtcttt atgccattac
     3841 taatttcatt tggacggtga gtttgtattt gtcgaacgcc acgggttatg tggttcaaga
     3901 ggcttacaaa ttagatttca tttcggatac ggctgaaagt attggaaaga atatccaaac
     3961 attggctggt attactgaaa atggactcca gacctccgga ttttattttg ggtttctatt
     4021 attgatgatt ttagctctgg gggtgtatgt ggcgtatacc ggattattaa aacgagaaac
     4081 cacgaaagca gttcgagccg tgattaattt tgtgatgatt tttctactat cgggttcgtt
     4141 cattgcttat gcgcctacct atatcacaaa aatcaatgac tttagctcag atgttagtga
     4201 agcggccctt acccttggaa ccgagatcgt tgtacccaat tcagaaagcc aaggaaaaga
     4261 tagtgtggat ttgattcggg atagtttgtt ttcgattcaa gtccaacaac cttggttact
     4321 actgcaattt gatgattcca acatagaaga aattggtgaa gaccgggtca acaagattct
     4381 atctgtgagt ccagatgaaa acaaaggcaa agatcgggaa gaagcggtca aggcagagat
     4441 tgaagacaat gacaatgcga atctcagtat taccaagacc atgaaccgtt tagggaccgt
     4501 ggtctttttg gtattgttca atattggcat ttccttcttt gtttttcttc taacagggat
     4561 tatgttgttc tcgcaaattc tcttcattat ctttgcgatg tttctgccaa ttagtttctt
     4621 attgtccatg ttgccgactt atgaaagttt aggaaagaag gcgattattc gtttattcaa
     4681 tacgattatg atgcgagcgg gtgttacttt ggtgattacg actgcattta gcatctccac
     4741 gatgtttttc aatatttcgg caacctatcc atttttcatg gttgcttttc tgcaaattgt
     4801 gacctttgcc ggtatctatt tcaaattggg cgacattatg agtatgttca acctccagag
     4861 taatgatagc cagtcaatgg gaagacgcgt catgcgaaaa ccgcaaatgt taatgaatcg
     4921 aaaattgcgg caactcaatc gaaatgtggg acgagctttg gcttttggtg gtggtgcagc
     4981 agtcggaaat aagttggcaa aggaacaatc aaaatccaaa tttaagccat ccggttcttc
     5041 tctacgaaag aattcacgat taccgaacga tcacgaaata ctatctgact taactaagga
     5101 gaatccagtt tcaaataata agaaacaatc ccgaatgaat ttggctggga gaaaaatcgg
     5161 caaagttttg gatacccaag ctttggtgaa agataagact aaacaagtaa aggatcaggt
     5221 aaggaatacg ccaacgaatt tgaaatacaa tctccataaa ggaatcgaaa agtcgaaaaa
     5281 agcaccagaa gaattcaagc gcggactgtt ccaagaaaaa gcggatcgag tggatttgcg
     5341 agaaaaacaa cgacaacgaa gaaatgcgaa aatggacgaa aaacgcaaga tactgagtga
     5401 agcaacaaat cgtcagggaa aaagaagggc gaatattcca atagataaag cagctcaacc
     5461 tcagaaagat atgatgctga aaaaagaagc accaaagcgg attgtgagag caaatctgaa
     5521 tcccgaaatc aaacgaaaac taactagtca agaaatcatt tcaaaaggga aaagccaatc
     5581 agcaagtaag aaaaattctt tccaacaagt gaaacagagg tccactctgc caaagcagac
     5641 caatcaaaaa gtacaaaaaa gaatgaacca tcgtccaaaa tcaaggtcag gtgagaaaaa
     5701 atgaacttga agaaagtggg tgttatttca ttaacttttc tgaccatctc ttttatgggt
     5761 attttgatgt gtgtgggttt gttgtttggt gaagacagtg agggaaatgg tagttctggc
     5821 acttctcctg gcggaactag tgtctcagaa gaagtattga aacaccgtgc aatggtggaa
     5881 aagtatgcca aagaatcgga tataacagag tatattccaa ttcttttggc gattattcaa
     5941 gtggaatctg gtggcacaat ggaagacgtt atgcaaagtt ctgagtcagc cggattacca
     6001 cctaattcat taagtaccga ggcatccatc aaacaaggct gtctctattt tgcgtctttg
     6061 gttaagcgtt ccaaagaact agggtgtgat caagatagta ttattcaggc ctataactat
     6121 ggtggcgggt atctagatca tgtcgggaaa aacgggaaga agcatagttt tgctttagct
     6181 gaatcttttt caaaggaaaa atcaggtggt caaaaagtcg attaccccaa cccaatcgcg
     6241 attaaggaaa atggtgggtg gcggtataat tatggcaata tgttttactg tttattagtg
     6301 aagcaatacc taactacaac tcagtttgat gacaaaatag tccaagaaat ttttgatgag
     6361 gcatttaagt atgaaggaac ggcctatgtg tttggtggct caagtccaga aacggggttt
     6421 gattgtagtg gcttgaccca atggtgttat gccaaagttg acctgaaact tcctcgggtg
     6481 gctcaggatc aatatgatgc catgactcat attgatctaa aagatgccaa accaggtgat
     6541 ttagttttct ttcattcaac ctatgatgcc ggtacttatg tgacccatgt gggcatttac
     6601 gcaggggaga atcgcatgtt tcatgcagga gatccagtgg ggtggacgaa tctaacggaa
     6661 aactattggc ggcagcatct tatcggagct ggccgataca aataagagaa aagaggaaaa
     6721 agaatgaaga taaaaattga acggaatcaa aaggaaaaga gcccaaaaaa gaaaaaagaa
     6781 cgagttgtat ccgtgggtaa gcatcgaaaa atggtgcttg ccctttggct tttattgtct
     6841 tgtagtcttg cttttggcgt ttataaaaat ttcaccgcca ttgatcaaca tacaactcat
     6901 gaaaaagtgg ttataaaaga gaaggtagtg aatacttctg gcgttgaaag ttttacagag
     6961 gattttgcga aagaatattt ttcatggaaa aataacaaag aagcaattga aaagagaatg
     7021 agtaatctcg aacagtacct tacagaaaac ggtctcgctt taatccaaga tatggttcga
     7081 gcagatattc ccacgagttc cgaagttcaa tctgtaaaaa ttctagatgt tgaaaaacac
     7141 tcggaagaat ttgtcgtttc atttttagtg gagcaacata ttacagaggg aaaaaagacg
     7201 caatcaattt cttctgcata tcgagtgaca atctttgaag atgaaaacag gaatcattta
     7261 gtaactagtt tgccaacaat gattggaaag ccagacaagg caaaatatga agcaaaacaa
     7321 gtagaaaacg attcagaaat tgatgccaaa acaacggaag aaatcaccga ttttttagaa
     7381 acatttttta aactttatcc atcagcttct gaaaaagaat tggagtatta tgttgaaaat
     7441 ggtgcgatgc gaccaatcaa tattgactta aagttttcgg agttagagaa tccagtttat
     7501 ctacaaaacg gaaaaaattt tctagtgagt acaaagataa aataccttga taatgtatca
     7561 aaaaccacta gctactttca gtacaaatta gtattacaaa aaaatggaaa ctggaaaatc
     7621 gttgatgtta aataataatg actcaatcta attttattag aagttataag tagtgtaact
     7681 tattt
//
LOCUS       DACCXM010000121         7639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCXM010000121 DACCXM010000000
VERSION     DACCXM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.125"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            17..2344
                     /locus_tag="IVY61_002351"
     CDS             17..2344
                     /locus_tag="IVY61_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7971424.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2470..2799
                     /locus_tag="IVY61_002352"
     CDS             2470..2799
                     /locus_tag="IVY61_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP7971425.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2839..3843
                     /locus_tag="IVY61_002353"
     CDS             2839..3843
                     /locus_tag="IVY61_002353"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7971426.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3840..4286
                     /locus_tag="IVY61_002354"
     CDS             3840..4286
                     /locus_tag="IVY61_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP7971427.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4436..5251
                     /locus_tag="IVY61_002355"
     CDS             4436..5251
                     /locus_tag="IVY61_002355"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7971428.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5457..6629
                     /locus_tag="IVY61_002356"
     CDS             5457..6629
                     /locus_tag="IVY61_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7971429.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6839..7285)
                     /locus_tag="IVY61_002357"
     CDS             complement(6839..7285)
                     /locus_tag="IVY61_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971430.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(7282..7476)
                     /locus_tag="IVY61_002358"
     CDS             complement(7282..7476)
                     /locus_tag="IVY61_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971431.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2572 a         1443 c         1523 g         2101 t
ORIGIN      
        1 tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc aacgtatatg
       61 tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact tacatcccat
      121 tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta tttatgtaaa
      181 cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa atagtccacc
      241 ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg agacaacctc
      301 aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag cagcaaaagc
      361 agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta ctgttgcggc
      421 cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat atgccaaaga
      481 tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt tacaaggcgt
      541 aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt ttattggctc
      601 cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg tggctaccgc
      661 tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt ttacaccctc
      721 aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg gtatacagtt
      781 agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg ttgatggtaa
      841 gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca atgcgaatcg
      901 gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa cgtacgggac
      961 tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag cagtaacggg
     1021 ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg tcgatacggt
     1081 gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt ttggacagat
     1141 gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag acctgacgac
     1201 taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgatag aaacaaagaa
     1261 ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa tcgtaaagga
     1321 cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg caacagtatc
     1381 aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga tcgttaaata
     1441 tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca gtattgatca
     1501 aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg aagtaactgc
     1561 gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac tagctaaact
     1621 agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct atacgattaa
     1681 gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa aagcaggtgt
     1741 cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc cagtacaagt
     1801 ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata tggttaatgc
     1861 tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag cagcaaatcc
     1921 caagttgtca tttactgata tagccaacac atcaggaact tataccatct ataaagctaa
     1981 cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc actcgatttc
     2041 ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt ataccctaac
     2101 agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag gcgaaggaag
     2161 tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga tcaatccgac
     2221 tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt taggactgat
     2281 ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag gtgaacagcg
     2341 atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt tatcctactg
     2401 gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac atttactttg
     2461 cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga cggaataaat
     2521 atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc tatatatgat
     2581 gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga cgcacaaaga
     2641 aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac aaaaacagta
     2701 gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca aggtcgtgac
     2761 gctgtttacc gatttacgga aataaagact tcagattaag taaaagaaaa atcagcacca
     2821 tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac catccacttg
     2881 tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac agtagagaat
     2941 aagcagacag attttgcaga tggggataaa gtcccttatc tcatcaccgt aacaattcct
     3001 gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc tcagttatgg
     3061 ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac atttcacaca
     3121 caaaaaataa aacatggttt tgtcttatca gattctggaa aagatcttag ttcgtttgct
     3181 ggaaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga aaaaacgaca
     3241 tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca tcttgccatc
     3301 caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa actagcagat
     3361 gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg gataaatcat
     3421 tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga tggaacattt
     3481 tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc gcctaaagga
     3541 tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta tgatccgctt
     3601 catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa taatggaaaa
     3661 acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg aagctttcca
     3721 aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttattgct agtcctttca
     3781 acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag ggaaataaaa
     3841 tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg ctgcttcctt
     3901 tcgttctcgg cctaggaact gcagaggttt ctgcagccgt atcacctaca ccagaaaatg
     3961 ttacggttaa tttgcataaa ttgaaattca cttcagcacc agaaaatcaa ataaacaacg
     4021 gaacggaatt gacatttccg aatagcgaac cgttgaacgg cgttgaattc aacgtatatg
     4081 acatcactgc tacgtactat ccaagtaaag acacagcagt tccagcagac gctacaccct
     4141 tcgcaagtgt aacgacttct ggtgaaggat tagctaacct gactcttcca ggtaaatctg
     4201 atgggaaaga tgctgtatac gtctttgtag aaacaccaaa gcccggtgtc gaaacgtcgc
     4261 caaatatcgt gttaagctta ccctagaccc tgacaaacct ttagacacga ttcacctata
     4321 tgctaaaaat gtcatcaaag aaagcaatat caaaatccag aaagctggga acttttcagg
     4381 ggtagataag ttagcagggg cgcaattcca gatcaaaaat tcagagaatc aattcgtgac
     4441 cggttttgat gaaaacactg gtgtcgctct ctatgatcct gctgagtcag cgttgacatt
     4501 cacaactgat actgaaggat ccatcagtgt caatggatta atagacggca attatacttt
     4561 agttgaaaca aaagcaccag atggttatca attaaccaat ccacaaaaag aaactacttt
     4621 tatagtaaaa aacggaaaat tgcaacaaac aaccggtatc atccatggcg cagatgaagc
     4681 caatcttggg aatccaaacg gtgtagtcaa tcgggaaaat acaactacgg ataacacgat
     4741 tactgtagaa aatgtccaaa actacggtga tttccacttc atcaaacaag atgtgaacac
     4801 gcaagaaaaa ttagccgatg cagaattttc tattctaaga gcaaaagatc caactgttaa
     4861 attaatgaaa aaaatagcaa accttgcctc aggagaatac ccatatgctt ggtcagatga
     4921 agtcacaggt tctgactggg aagaagtgaa attgatttct gattcagaag ggaaattttc
     4981 aatcgaaggg ctgaaacaag gggcatactg ccttcaagaa acaaaagcac cagaaggcta
     5041 cgcattacct aagaatgaag ctgcgtacac tgaatttaca gtagatgatg atccaacaac
     5101 ttctgatgaa acaacgatcg ataaccaacc taaaggaatc ttgccttcaa ctggtggtat
     5161 ggggatcatc gtattcatct tggtaggtac tgctttagtt ggaggagcag tgatctattt
     5221 caaaaaacgc catcaagaaa cagaagctta aactattgga aacaaaaatc atacgataaa
     5281 taaacgatag tgattcaaat ggggagaaag gttgtgacaa gtggtcggtc tcttggtatt
     5341 cgctcaacaa acggcaaaat aggaggaact attttgccgt ttgcttcgct aatactcagg
     5401 tcaaggcgct gatttcacaa cctcttcata tacatattag aaaataggtg gacatgatga
     5461 ataaaagcag aataagaata ctggacatct tcatggcaat cattttagtc gttgggatcg
     5521 ggatcttttc ttatcctttt gtagaagatt cactgaatga ttttcttgcc cagcaaatga
     5581 tcatccatta tcaaaagcaa gcatcgaata agaattcaga ggaaataaaa aaacagcagg
     5641 aaaaaatgac aaagaaaaac caacagttgg cagagcaaaa cgtatcacca gggataggca
     5701 gtttcaacca agcagttgat gcaaaagcat taaaagattt acctagtaat gcctttttta
     5761 tggaacatat gcttggagtc atcgaaatcc caaaaatcaa tgtcagctta cctatattcg
     5821 atcagacaac ggaaatattt ttgcaaaaag gaacgtcatt attggaagaa agttcttatc
     5881 cgactggcgg gaaaaacacc catgccgttc tttctgggca tcgtggactc ccagaagcaa
     5941 aattatttac agatttgcct aagttgaaaa aaggagacca gtttttcatc cagatcaatg
     6001 gaaagacatt ggcgtatcaa gtagaaaaaa tccaagtggt cttacctgat gaagtagatt
     6061 ctttgggtat ccaaaaaggc agagatctcg tgactttgct tacctgtact ccttatatgg
     6121 tcaatacaca tcgactgctt gtgacggggc atcgtattcc ttatcaagca aaagaggcaa
     6181 aaaaagcgat ccaaggaatt gatcaatgga aaaaatggaa attttttatt tggtttatcg
     6241 gtatactact tggatcaatc ggcttagttt ggctactcat cgcttattta gattctttgg
     6301 ccatcgctaa gcgaaactat cctttatctt tttatgtaaa gaacacaaac ggacgaccaa
     6361 tcgaaggaat ggtcttttct gtaaaaacgc tcaacggcaa acactatatt actagagaaa
     6421 aagttccatt tgtgaaagct tctgatgaat acggactagt gagattttct gatttaaaag
     6481 gcaggaacta tcgcttgcaa catgaggagc tcctgcttaa aatccatgta aaacataaac
     6541 attcgaaaca gttttcaatg aaattgaaaa aaggaagata taaactgcga aaagaaaaag
     6601 aggtgtacta tttgattgag aaagaatgaa tgaagatgtc caaaaaagca gagggaacag
     6661 actaaatgaa aaaagagaga agctgacgaa atggacgtgt aaactgttat ttttggttta
     6721 ctatctggaa cgccatcttc tattttttta tgtttcagaa gaattttatt attatagtat
     6781 aacttttgaa ttagtttcca taacatttgg agcaaggggt taaaccacga gcttgagctt
     6841 agcttaaagt ggcaggggta tacgtaccat ttccacattt atgtgtatgg tattttgaac
     6901 cggtcggtgt gacatagaca gtttgttcat tattttgctc aggagctgcg gcttgtgcct
     6961 gctgagaggc ctgttcagct gctgattgct gtcgcgcctg ttctgcttgt tgctgtgcga
     7021 ttgcttcttg cctcgcctgt tctgcttgtt gctgtgcgat tgcttcttgt tgcgcttgtt
     7081 ctgcttgttg tcgtgcaatt tcttcttgct ttttccgttc ttcttctgct tcttttgctt
     7141 tgattgtgga atcaattgca gcaagacggc ttgtcaactc ttgtttgttt tcaggtagcg
     7201 cttggatagc tgctgaagca gttgcatagt tttcttttgt aggatttgct tcagcttgtg
     7261 acaaagcaga ttccgcttct gtcacttttt ttctagtgca gctttttccg ctttttgttt
     7321 ctcttctttt ttcttttcaa tttgttcttg ttctttcgat gaatctgccg tactttcgga
     7381 gatcgtggta ctgctgctgg aagcctgatc tgaacttgtg ctttgagtag tagacgtagt
     7441 agcaccacac ccactcaaca aacttcctgc gaccaaaaac aatgcaacta attttttcgc
     7501 taaatttcct ccttaagtaa aaaacaaccg ttttcaataa atgtcctttt caattgttta
     7561 cttacttgtt ataataattg tgttatgaat agtgattgag gaaacttaat ctaatcccgt
     7621 gttgatacac gggattttt
//
LOCUS       DACCXM010000122         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCXM010000122 DACCXM010000000
VERSION     DACCXM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.126"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(138..428)
                     /locus_tag="IVY61_002359"
     CDS             complement(138..428)
                     /locus_tag="IVY61_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971432.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IVY61_002360"
     CDS             complement(546..806)
                     /locus_tag="IVY61_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971433.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IVY61_002361"
     CDS             complement(943..1356)
                     /locus_tag="IVY61_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP7971434.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IVY61_002362"
     CDS             complement(1812..2297)
                     /locus_tag="IVY61_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971435.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IVY61_002363"
     CDS             complement(2436..2651)
                     /locus_tag="IVY61_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP7971436.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IVY61_002364"
     CDS             2875..3675
                     /locus_tag="IVY61_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7971437.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IVY61_002365"
     CDS             3694..5562
                     /locus_tag="IVY61_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7971438.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IVY61_002366"
     CDS             5555..6046
                     /locus_tag="IVY61_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP7971439.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IVY61_002367"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IVY61_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP7971440.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IVY61_002368"
     CDS             6606..>7600
                     /locus_tag="IVY61_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP7971441.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACCXM010000123         7497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCXM010000123 DACCXM010000000
VERSION     DACCXM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.127"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(161..886)
                     /locus_tag="IVY61_002369"
     CDS             complement(161..886)
                     /locus_tag="IVY61_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971442.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1040..1366)
                     /locus_tag="IVY61_002370"
     CDS             complement(1040..1366)
                     /locus_tag="IVY61_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971443.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1403..1768)
                     /locus_tag="IVY61_002371"
     CDS             complement(1403..1768)
                     /locus_tag="IVY61_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP7971444.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1805..2020)
                     /locus_tag="IVY61_002372"
     CDS             complement(1805..2020)
                     /locus_tag="IVY61_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971445.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2149..2484
                     /locus_tag="IVY61_002373"
     CDS             2149..2484
                     /locus_tag="IVY61_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971446.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2560..4557)
                     /locus_tag="IVY61_002374"
     CDS             complement(2560..4557)
                     /locus_tag="IVY61_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971447.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4655..4930)
                     /locus_tag="IVY61_002375"
     CDS             complement(4655..4930)
                     /locus_tag="IVY61_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7971448.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5162..5896
                     /locus_tag="IVY61_002376"
     CDS             5162..5896
                     /locus_tag="IVY61_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971449.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5935..7077
                     /locus_tag="IVY61_002377"
     CDS             5935..7077
                     /locus_tag="IVY61_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7971450.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2521 a         1370 c         1086 g         2520 t
ORIGIN      
        1 tgaaactatc tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa
       61 aaaaataccc ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac
      121 aacggggtag ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa
      181 atacttcgtt gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc
      241 cattttcaag taaccgaagt ggaatatctt taccaacttt ctctgtcact gcattactga
      301 tacctgttag attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat
      361 cagtatttat cgtgattcct ttatcttcat ttacaagttt ccaagtaggt ggtatagctg
      421 tgtatgtttc tacaaatgaa ttaagttttt cattaagtga cttctctttt atatttccat
      481 ctaaaaactc tgtatatatg gcttgagcta tactaaaaca aagctggcct tgttctaata
      541 attttacatc attagttagt aaagctgtgt tcacgttatc aatcgcttca agaagagtat
      601 ctttggtgtc cctaacagct ttaggtgtgc cttctggaat ttccctactt gatacctcgt
      661 ccattttatc ttccataaac cccttaaaat catatctttc tcccaaatcc atagaagttt
      721 ttgagaaagc tttcatttct gagtctattt cttcaataat atttaacatg tctgatacat
      781 attgtttagg ctgattcgtt tttaatctat atgtggtaaa tatctcaatt cctccaatta
      841 tcaataaaat tgccaaacaa atccataata attttttctt tttcattttt cctcctatac
      901 actgctgtta taacagtgac taatgtaaag attttcccag caatcatcag actattattt
      961 acagggaatc aaaaaaactc cttagtaagc ttacaccttt aggagttcct gtttgaacgg
     1021 tatgcataat acatttaaat caatttaaca ttaacacctc agtatatggt atctctacat
     1081 atacacggtc tttttttgga tagtactcga catttatatt gatagcactt ccattgtcta
     1141 acgtagcttt agcccggtac gtgttatcac cattatcatg agtgatcgtc acatcatcac
     1201 atgtccgatt taagttatac tgttcttgta aaatctgagt taccaatggt acagatgact
     1261 tctctagtcc agatgaaccc gtcattcttg aaaaaattat acctataata gccaatacaa
     1321 cgatacctat aatgacaaac ttcttattca tatttacttt attcaacttt ttatctccta
     1381 tctccaaaat gattttctct catcagtaac tcattgacat ccccaatagt acaccaaaga
     1441 tggctccagc tacccctgct atcatcaaaa tcgtacccat ttgttcatct ttctctcgat
     1501 ataagtaacc cataataaca gcaacagcac caaataccac aggaataaat gctaatgata
     1561 ctaccataga aatccaacca ataatcaaat acagcttccc tgtctcttgt ccatgtacga
     1621 tttgttcctt catttcatta attgtctgct cagtactttt ctttactgtg tctgttccgg
     1681 ttgtctcatc tgtttcttgt aatgctccgc aaaactggca atacttactt tcatttgtta
     1741 tttctttacc acattttcta caatacatgt cttctcctcc taaaatcatt caattgctta
     1801 ttagctaact gtttacttta ttaaatacat cagaaaacag ctcatttatt ggtacactta
     1861 atatctcaga aagcattttc atttgatagg gtttaggaat cgttctccct gtttgccacg
     1921 aacttacaca ttgctgtgta acattcattt ttttggctaa ctcagtttgg gaaagtcctt
     1981 tttcttttct gtatcggctg agattactta cttttttcat gattctcccc tcctgtttta
     2041 ttatatacaa caatatgatg tatttcaact cataaaatat ataaatactt caaatgtcat
     2101 tacaaataca acaacttgtt gtactctact gagaaggggg gtgtattaat gaaacagaag
     2161 caatatgcaa aggtcattag agaaaagcga aaagcactcg ggtggacaca aaaagaatta
     2221 gctaaaaaaa tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagaa
     2281 cctaacctcg atagcttaac agctctttct agagccctag gaacccctgt aagccatttt
     2341 ctagacaatg ttgacataga ttatgaggaa gagtttttgg ctctctatcg ctctcttagt
     2401 actgaagatg caattcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa
     2461 cgcaatcagc tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt
     2521 attgagatga aataagatta agagctatgg ataacctgtc tagtttaata taattgggtc
     2581 tcttctattc cttactctcc gtccatcatc tcttgttaca cttgtaaatt gataaatagg
     2641 aactaattca actcctgata atatcaattc ttttattact tcatctatcc ctgccccttt
     2701 cgctagatat actaaaaaac tactgcttgc tttcacattg tatagtttct taatatcctt
     2761 agaagctcgt tcaagagtga atccttgttt aacaaagtag cctctgtttt ctattaaagc
     2821 atttttcacg ctgttatccc tgacatttcg taggcgttca caaacagtgt taataagttc
     2881 tttttctgtc attagatatg tcccttgcca cgataagccc tgtaaaactt catgtgcttc
     2941 atctaaaatc aattgttttt ccttgctaag ttctgatcgg gaattttgag gaaatactct
     3001 tttagcctct ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt
     3061 aaaatgaaat ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt
     3121 tgcattaaaa acgtctaatc gatagacatt gcttctctta tgtttaaagt ttcctaacgc
     3181 tttctcttcc tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc
     3241 taattttttt atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat
     3301 gtcataagtt ttaccatagt aagaactcat ttttttagct aattgttccc ccgacaaaat
     3361 acaagtcaca ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata
     3421 gacacgtcgc atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga
     3481 ttcaagagtg tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata
     3541 caagtctagg ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat
     3601 tagcatcaca actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa
     3661 aatggaatgc ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa
     3721 cagacatctt aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct
     3781 ttcataaatc tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg
     3841 tttcatttct tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag
     3901 ttcatactct ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat
     3961 cggaagctca gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt
     4021 atatcctcca aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt
     4081 ctttttcaag tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa
     4141 tagaaagaca atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc
     4201 aaacgcagaa ttatgaacta aattgataga taaaaccttt tctatgctta agtatctact
     4261 atcatcttta ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc
     4321 cagaataatt actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt
     4381 gcttggaata aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc
     4441 tctatagtta tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt
     4501 ctctaataat ttttctttat ctacataaat tggaatatta atcattgatg tttccatttg
     4561 tgtttcctcc ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc
     4621 tttattacta tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac
     4681 tatcaatatc agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt
     4741 attgagaaac atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct
     4801 gatagtccat atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca
     4861 ctgtaattat ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt
     4921 gtagagacat atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct
     4981 tcgtattttt tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat
     5041 gattaccagt tcatcgacat acacataata cctcgataaa aaaggggtgt caatagtttt
     5101 gtaaaattta tttacaacac cccttttttg aggtatacta attttaggag gtgtggtcaa
     5161 tatgataaaa cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat
     5221 aaaagctata agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa
     5281 cggtcaatta attgacaaaa agactatcta cgaatgggag aagggaacct atttaccaat
     5341 accagagcga ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg
     5401 tggcaatgta gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga
     5461 tggtataaca tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt
     5521 acactctaat ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga
     5581 atttattgct aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat
     5641 cttaaaaata gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct
     5701 tttcctcact agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat
     5761 tcaattgaaa gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa
     5821 caagctagaa caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg
     5881 aggtgatacc atctagtggt aagtactcct tgtaaactaa gaggagatga tttcatggca
     5941 aaaatatcaa agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt
     6001 gccaccgatc cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa
     6061 caagaagctc aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag
     6121 aaagcacctg atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc
     6181 agtgttaaag cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca
     6241 gcatttggta agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat
     6301 gaatggcaca gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta
     6361 cgctacgggg ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca
     6421 cgaaaaaaag agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta
     6481 ctggatgctt ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca
     6541 tacacaggta tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt
     6601 aataaagaac tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt
     6661 caatctccga aaacaagagc ttctagacga gttatctctt tagatactga gactctttct
     6721 atcctcaaca actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact
     6781 agctctaaag agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact
     6841 gttaatgatt ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat
     6901 gggtttagac atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt
     6961 caacaacgat tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca
     7021 aataatgcac ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag
     7081 agctggtcaa aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta
     7141 tatatcaagg gttctagctc ctataacagg tttacccaac actgccttcc aatgactctt
     7201 ttgggaatca gaggaaagaa tcaggcttta tagagatatt tcatctaaca aattacttta
     7261 gactgacagt atatatcagc aggtactgtc atcgggaacg tagccaatat cgtatccaaa
     7321 acagcaggta gagaagctat ttgaggattc aagttattct atttcaacca tgaagttaag
     7381 ttcattaaaa ataaagatag ttggtatttc aataattttt gtatagagtt aatcacttta
     7441 ttgatgctaa ttcatcttga ttggtattat aatcgtattc aagttgcgtg gcgtgct
//
LOCUS       DACCXM010000124         7323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCXM010000124 DACCXM010000000
VERSION     DACCXM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.128"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            33..506
                     /locus_tag="IVY61_002378"
     CDS             33..506
                     /locus_tag="IVY61_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP7971451.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            503..2635
                     /gene="feoB"
                     /locus_tag="IVY61_002379"
     CDS             503..2635
                     /gene="feoB"
                     /locus_tag="IVY61_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP7971452.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2637..2771
                     /locus_tag="IVY61_002380"
     CDS             2637..2771
                     /locus_tag="IVY61_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP7971453.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2865..3509
                     /locus_tag="IVY61_002381"
     CDS             2865..3509
                     /locus_tag="IVY61_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7971454.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3696..4889
                     /locus_tag="IVY61_002382"
     CDS             3696..4889
                     /locus_tag="IVY61_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7971455.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            4882..6609
                     /locus_tag="IVY61_002383"
     CDS             4882..6609
                     /locus_tag="IVY61_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7971456.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7003..7200
                     /locus_tag="IVY61_002384"
     CDS             7003..7200
                     /locus_tag="IVY61_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP7971457.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7202..>7323
                     /locus_tag="IVY61_002385"
     CDS             7202..>7323
                     /locus_tag="IVY61_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7971458.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEK"
BASE COUNT         2383 a         1257 c         1628 g         2055 t
ORIGIN      
        1 tacatgattt atttcacaag agaggaatca atatgcagac cttagcgcag gcaaagcaga
       61 atttcgttta tcaagtcgaa cgaatcgaaa cagaagaaaa aacaaaacgc catttgcaaa
      121 atatcgggat cgttgccgga agtaaaatcg tcgtcgtcaa tttatcaggc aacaatgcca
      181 ttttactggt aaaaagtcat cgagtggcag taagtcggga aatactaggt caaatctttg
      241 tcaataaaaa gactgcttcc tctgaaagct ggacttctct tgatcaatta aaagcaggag
      301 agactgggat cgtcgtagca atccacggac aaggcgcagt gaagcgacgg ttaatggata
      361 tgggattgac gcgaaatgta aaaatagtta tccgaaaact agcccctctg ggagatccaa
      421 tcgaactgac agtgcgaggg tatgaactga cgttacgaaa gtcagaggcc gaactaatct
      481 tgatacaaag ggaggaaaaa gaatgaatat cgcgttagtc ggaaatccaa acagcgggaa
      541 aactagcttg ttcaatctct tgactggttc gaatcaatat gtagggaatt ggccgggcgt
      601 aacagtagaa aaaaaagaag gatcgttgaa aaaaaatccg aacattcata ttcaggacct
      661 acccggcatc tattctttgt ctccttatac accagaagaa gtgattgcaa gagattattt
      721 ggttcaggaa cagcctgaac tgatcatcaa cattattgat gctaccaatt tagaacgaaa
      781 tctttattta actacgcagt tattggattt agacattcct atgatcattg gtctgaatat
      841 gatggatgtc atcaaaaaag aaggaaaaaa aatcaatcta gaaaagcttt cctatggtct
      901 tggtgtacca gttttaccaa tcagcgtatt gaaaaagcaa ggaatcgaaa aactaatcga
      961 aaagattcag gagtctgcaa aaaagcggcc ttctattcct gttccctgta catatgatcc
     1021 acgtttagaa gcagctttac atgaaattga agatgtacta ggagaacaaa gcggcaagaa
     1081 acgctggtat gcaatgaaat attttgaacg agatcagaag gtaagagagg attacgagat
     1141 agcagtttct cagcaaaaag agatcgagca actgatccag ttaacagaga aactattgga
     1201 cgacgatagt gagacgattc tggtcaatga acgttatgaa tttattaccc aactatgtac
     1261 actctgtgtt gtgtcaaatg attctttcca attatcgatg agtgacaaaa tcgatcagat
     1321 tgcaacgaat cgttggttag ccttgccgat cttcgcattt gtgatgtggc tgatctatta
     1381 tctggctatt caaacagtag gaacaatggg gacagattgg atcaatgaca cattgtttgg
     1441 tacatggtta ccagaacatg ttggcagatt gttagctgaa tggcaagtag cgggatggat
     1501 gcaggagctg atcttgaacg gcatcatcgc aggcgtggga gcagtgttag gatttctacc
     1561 gcaattgatt atattgtttt tatgcttgag ctttctggaa gactgcggat atatggcgag
     1621 gatcgctttt gtcatggatc gacttttcag gaaattcggt ctttcaggaa aatccttcat
     1681 tcctatgctg attgctacag gatgtggagt gccaggcgtc atggccagtc gcacgattga
     1741 aaacgagcaa gatcgtcgtt tgacgatcat ggtcacaacc ttcatgcctt gttctgcaaa
     1801 gttgccgatt attgccttag tagcaggggc cttttttcct catcaatctt gggtagctcc
     1861 ttctgcctat tttcttggta tgactgcaat tattttctct ggaatcagct tgaaaaaaac
     1921 acgtttgttt gctggagata cagctccttt tatcatggaa ttgcccgcat atcattttcc
     1981 tcaatggtca gcggtcctaa gacaaaccta tgaccgcagc aagtcttttg ttaaaaaagc
     2041 aggaacgatt atctttgttt ctagtatcgt gatctggttc agctcttctt ataattttta
     2101 tgctcaacct gtttctgaag accaaagtat cttagccttt ttcgggcgaa tcctggcagt
     2161 tgtttttcag ccgcttggtt ggggaaactg gcaaggaaca gtcgcagcga ttacaggatt
     2221 ggtcgctaaa gaaaatatta ttggcacatt tggtattttg tttggtcatg cggaagtatc
     2281 cgaaaatggc agagaaatct ggcaagtgct acaacacaca tatactcccg cagcagctta
     2341 ttcattgtta gccttcaatt tactttgtgc gccttgtttt gcggcaattg gggctatcca
     2401 tcgggaaatg aatgccaaga aatggacttg gatcgccata ggctatcaat gcggtttagc
     2461 ttatttagtt agttttgtga tttatcagtt gggacactta ttggagggtg gacaggtagc
     2521 gttagggacg tatcttgcta ttttactgat gataggactc gtttatgttt tgttccgtca
     2581 gccaaaatcc aagaaccagt tttatactat attgtcatta gaaggagagg aataggatgg
     2641 cgacattctt attaagtgtg ttaatctttg gaagtgcagg cgcaattgtc tatactcgtt
     2701 taaaatccgg taagaattgt gatgactgtc aaacagcttg cccagtaaaa aaggaacaga
     2761 tcaagcgatg atcagtcgtt gcaagaggct attctcccct tttttgctta ttttgctaga
     2821 gcatagtaag atgagggaaa gtaaaaaaag aaagaggaaa gaaaatgtct ggaaagaaaa
     2881 aaatcgtccg cccgcgctac cagcagattg ctgttgattt agccgaacgt atcgttgaaa
     2941 accgatataa agtgggtgag aagatccatg cccgctcaac acttgccagt acgtacaatg
     3001 cttctcctga gactacccgc aaggctatca atgtgctggt agatcttgga atcatggaag
     3061 tacggcatgg aagtggggca tttgttgctt cgaaggaaaa agcaaaagat ttcctattgc
     3121 aatatcaaga tgtacaatcc atccaagata caaaagcaga aatcatggca agtgtggaga
     3181 agcaacaaga tgagctgaat catttttctc aattattgga tcagctggtc aatcaaacga
     3241 agcgaataca taagatgaat cctttacttc cgtttgaatt atctttatca gaaaaagcaa
     3301 tgcatctgga ccgttcaatt agcgagttga atatctggca ggcgactggc gccacagtta
     3361 tcgcaatttc tcataatgaa gaactgcttg tttcacctgg cccctatgct aaatttacgg
     3421 caggggacat cgttcatttt attggaaatg aatatacact acagcgtatg acaaactttt
     3481 tttacccgga gcatcagctt gcagaatgat tgtgtgaaat attaaaaaaa tattaacttg
     3541 gctaaacctt ttatttacaa agatttagcc aagttttttt cagtttggac aagcaaattt
     3601 ctttcatttt tgacaaagtg actacatgat gttaacatag tgtggtcact tttttcagaa
     3661 agaaaaatga atttacataa aaaaggagaa tttgtttggt aaaagtagaa gtaaaacatt
     3721 taacaaagat atttggtaaa aagacacaag ccgctttaga tatgatgaat gatcaccagc
     3781 cgaaaacgga gattttaaag aaaaccggtg caacggttgg tgtgtatgac gtgaattttg
     3841 acgtaaaaga aggagaaatc ttcgtgatca tggggctttc cgggagtggg aaatccacat
     3901 tgatccgtct attgaatcgt ctgattgagc caacttcggg cagtatctat attgatggag
     3961 aagatgtatc gaagttaagc aaagaagaat tgcgagaagt ccgtcgtcat aaaatcaata
     4021 tggttttcca aaacttcggt ctgtttccac atcgaacgat cttggaaaat acagaatacg
     4081 ggttagaagt ccgtggtgta cctaaagaag aacgacaaga aaaagcagaa aaagcattag
     4141 aaaattctag tttgctttca tttaaagacc aatatcctag tcagttatct ggtgggatgc
     4201 aacaacgggt tggcctagcc agagctttag caaatgatcc tgagatcttg ctgatggacg
     4261 aagcgttctc ggctcttgat ccgctgatcc ggcgagaaat gcaagacgaa ttactggacc
     4321 tgcaagccaa tgttcaaaaa acgattattt tcattaccca tgatttgaat gaagctttac
     4381 ggattgggga cagaatcgcc ttgatgaaag acggcgaaat catgcagatc ggaacaggtg
     4441 aagaaatatt gaccaatcca gcaaatgatt atgtacgtga atttgtggaa gaagtcgatc
     4501 gttccaaagt gcttacggct caaaacatca tggtacctgc attgacgacg aatatcgaat
     4561 ctgatggacc aaacgttgca ttgacaagga tgcgtaatga agaagtcagc atgctgatgg
     4621 ctgttgacag aaagagacac ctgaaaggga tcatcactgc agatcaagcg ttagaagcta
     4681 ggaagcaaaa acgtccattg atcgacttcc tagatgaaaa tgtgacggtg attgggaaag
     4741 acatggtggt aagtgatatt ttcaatatta tttatgactc accgactcca cttgcagttg
     4801 tagaggatgg aaaattaaaa ggtgtcgtca tccgaggtag tgtgattgaa gcattagcag
     4861 aaacaagcga ggtgagtgaa catgaataat ctattgaact atcaattacc agtggcaaac
     4921 tgggtagaaa atatcacaga atggtttacg acaacttttt ctggcctatt ttcattttta
     4981 caaacgattg gacaggcagt gatgtcgggg attacaaatt tactgttagt gattccagct
     5041 cctttgttta tcttgttatt gacggtagca gctttcttta tttctaagaa acgtccagga
     5101 cttacgttgt tcacattaat cggtctatgg tttatttata accaaggtct atggaatgat
     5161 ttgatgaata ctgtcacact tgttctttta tctagtgtga tctcgatcat cattggggta
     5221 ccattaggga tcttgatggc aaaaagcagt aaagcccaaa gtattatcaa accgatcctt
     5281 gactttatgc agacaatgcc aggattcgtt tatttgattc cagcagttgc atttttcggt
     5341 atcgggatgg ttccaggggt atttgcctca gtcatctttg ctctgccacc aaccgttcgt
     5401 tttacgaatt tagggattcg acaagttcct aaagaattag tagaagcttc tgattcattc
     5461 gggagcacaa gtcgtcaaaa actattcaaa ctagaattgc ctttagctaa aagtacgatc
     5521 atggcaggga tcaatcaaac aacaatgctg tcactttcaa tggtcgttat tgcctctatg
     5581 atcggtgcac ccggcttagg acgcggcgta ttatcagcat tgcaaagagc acaagtcggg
     5641 aatggatttg tcaacggggt tgcattagtt atcttagcaa tcatcattga ccgttttaca
     5701 cagcatttga accagccaaa caataaaaaa gctgcaggag caacaacaca gaaaagtaaa
     5761 aaacgtcaag gactgatcat tggtgctgta gtagtagtga tcttaggtgc gattggtata
     5821 gggagttttt catctgccaa agaaacaaaa cgtatcaact tatcttatgt tgaatgggat
     5881 acagaagttg cttcaacaaa tgtagtcggt gaagtattga aacaaatggg ctatgatgta
     5941 acgatgacgc cattagataa ttcaatcatg tggaaatctg tttcaaatgg cgagtcagat
     6001 gccatggttt cagcttggct acctaaaact catggttcac aatatgcaca atataaagat
     6061 caagtcgaag acctaggtgc caatttgaca ggagcaaaag ttggtttagc agtgccagcc
     6121 tacatggatg taaattcgat cgatgagtta accgatcagg caggtaagaa aatcatagga
     6181 atcgagccag gagctggtgt agttacggct gctgaaaaca cgatccaaaa atatgacaac
     6241 ttgaaagact ggaaagtaga gacatcttct tcaggagcaa tgaccgttgc actaggtcaa
     6301 gcgatcaaaa aacacgaacc aattgtagtt acaggatgga cacctcattg gatgtttgcc
     6361 aaatatgatc tgaaatactt agaagatcct gaaaacggaa tgggaagcga agagcagatc
     6421 catacgatgg ttcgaaaagg attgaaagaa gaccagccag aagcttacaa agtattagac
     6481 aatttccatt ggtcagaaaa agatatggaa aaagttatgt tggaaatcaa caatggaaaa
     6541 gatccacagc aagcagcaaa agactggatc aaagagaacc aagaattagt cgaaagctgg
     6601 aaaaaataaa aagacagaca cgaaataaat ggagcttcca aaattggatc aaatatccaa
     6661 atttggaagc tccatgttcg tctctatctt ttttgtctat aaaaaaagag aatgcgtagc
     6721 tcatcttcga aacgttcaaa gacgccgaca aactattatg aatttatggg tgcgagtcaa
     6781 agagatcgat cgtaggtatg aaagactaat gtgattaaag tttattatgt acgaagtgca
     6841 ttctcaaaaa atattaaaga atatacatag tttatatact taaaagtgat taaattctta
     6901 ttcaggaatt attttgctac gtttcaagaa ggaattgttt tttgaaattt aatgaatgtt
     6961 agaataatag tctaaaaaca ttaatttagg ggtgattaga ttatgaaaca tttaaaaatt
     7021 ttgtctatta aagagacaca acttatctat gggggtacca ctcatagtgg aaaatattat
     7081 ggaaatggag tgtattgcac taaaaataaa tgtacggtcg attgggccaa ggcaactact
     7141 tgtattgcag gaatgtctat aggtggtttt ttaggtggag caattccagg gaagtgctaa
     7201 aatgaaaaaa aatgctaagc aaattgttca tgaattatat aatgatatat ctataagtaa
     7261 agatcctaaa tattctgata ttcttgaggt tttacaaaag gtatatttaa aattagaaaa
     7321 aca
//
LOCUS       DACCXM010000125         7294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCXM010000125 DACCXM010000000
VERSION     DACCXM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.129"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(205..1653)
                     /gene="cls"
                     /locus_tag="IVY61_002386"
     CDS             complement(205..1653)
                     /gene="cls"
                     /locus_tag="IVY61_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7971459.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1805..2698)
                     /locus_tag="IVY61_002387"
     CDS             complement(1805..2698)
                     /locus_tag="IVY61_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7971460.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2682..3368)
                     /gene="ftsE"
                     /locus_tag="IVY61_002388"
     CDS             complement(2682..3368)
                     /gene="ftsE"
                     /locus_tag="IVY61_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP7971461.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3473..4574)
                     /gene="prfB"
                     /locus_tag="IVY61_002389"
     CDS             complement(join(3473..4510,4512..4574))
                     /gene="prfB"
                     /locus_tag="IVY61_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP7971462.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4700..7234)
                     /gene="secA"
                     /locus_tag="IVY61_002390"
     CDS             complement(4700..7234)
                     /gene="secA"
                     /locus_tag="IVY61_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP7971463.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1936 a         1629 c         1277 g         2452 t
ORIGIN      
        1 tgctatactg gctttgaaat gtgtctcttt taatagcata tattagctta tttcttgctt
       61 tccccaaaaa acaagagata ccccaaagtt aatttcgctc atgcgagacg ctttctccat
      121 ttctgacgta ctctcagaaa tggccccgtt aaaaaaggac acgagatctc catctgttga
      181 tggttctcat gtccttttta tgttttaaag gagcggcgaa atcaatcttg ctaatccttc
      241 tttcatcttg atgattattc ctcggttttc atacatttct tttgtgagat gcgtagaaat
      301 tttcgagtct tcaaaaaaag cttttcttac acgtgaagcc attcgttcat cataaaccag
      361 tgtattgacc tcaaagtcca agcgaaatga acgaacatcg atatttgctg agccaactga
      421 aacaatgcca ccatcgataa tcatcgtctt tgcatgaata aatccattct catatgtttc
      481 gatccttgct ccatactcga tcagctctgc agcgaaagaa tacgttgccc agtaaacgag
      541 aagatgatct ggtttgttag gtatttggat atgaactttt acgcctgaaa gaagagccaa
      601 cttcagtgct tcatgaatcg atccatccgg tatgtaataa ggcgtttgga tcaatatctc
      661 ccgtttggct aacgaaatca ttttcaaata agtcattttg atttgttcat gttcggtatc
      721 tggtccgctt gtcacgattt gaacagctat atcaccagta gtgtgaatgg acggaaaaaa
      781 tttgggagaa tagcctactt cttttgtatg ctgagaattc cagtccatca aaaaacggtt
      841 ttgcaaggta tacaccgctt ctccgtaaat acgcaaatga ttgtctcgcc aataaccaaa
      901 tttctttacc aatcctagat attcatttcc aacattgaac ccacctgtat atccgatttc
      961 cccatcaata actacgattt tacgatgatt gcgataattg atccgaggat taatatacgg
     1021 aacaaataga ggaaagaaaa atgcgacttc tccgcctgct tttttcaaat tctgaaaaaa
     1081 gctggaagaa acttgtgtgg aaccccatgc atcaagaagg actctgactt ttacccctcg
     1141 tcttgccgcc tctgtcaatg catctcgaac ttcttcacct aatgcatcac tgcgataaat
     1201 ataatactgg aagtggatat ggtcttctgc ctgcttgata tcttcaatca gcgcatcgaa
     1261 tttttcacga ccatctgtaa ataaagtgat ttcattttta gtcgtataga gcgtactttc
     1321 aaataccgtg agcatataga tcaattgttt cacatctact tgtccagtag gcggatgagg
     1381 ataaagccct cgcatcaatg cctgtttgtc ttcttcaatt tccaggttct taccgatttt
     1441 cccttggatc ttcaaatcaa agattttctc tttggaaatc cctcttccta aaaaaatata
     1501 taaaataaat cctacaactg gaatgaacat caaaacgagc agccatgccc atgtttgagc
     1561 agtttccttt ctttctcgaa aaacgatgat aaaggaaagc aaaatattca taatcaaaag
     1621 gatcgtaaaa aaattgtcaa cgataatttt cacggcttca cctcttcttc taccaactta
     1681 agaataccca agttgttttt taaagtaaag ggaaaaaagc cgcttccttc tattctccta
     1741 gccaataaaa aaagaaagct ggaacgaaac tgtccaactt tctcatttta tttatattgt
     1801 ggtattagaa tttcaagaaa cgtctcatag aaatgatcga gccaagtgaa ccaatgatca
     1861 caccaatgcc gaccattaaa agattgattt tccaaacgat attttgtgga tgaatcaatg
     1921 aataatggga acgtaataat gatggattga tcaattggaa aaaccatgca taaccaaatg
     1981 tgatgatcaa aattggaaga attgcaccga tcagaccgat ccagccacct tcaaggaaaa
     2041 acggccagcg aatatatcca ttttttgcac cgactaaacg catgatttga atttcacgct
     2101 gtctagacaa aatcgtgatc cgaatcgtgt tcgaaatgag aaacatcgct acgaacagca
     2161 gcaatgccgc tgcaactaat ccccatgtcc gaactgcacc agcgatttgg aaaatccgat
     2221 ctgaggaagc tccaccgtaa tcagaacgag aaacattctt caaatctgct gcttcttctg
     2281 ctacttgttt tgtataagaa ggatcagtag cacttactac atacacatca tataacgggt
     2341 tattgtcacc ttcaaacaat ttccaggaat cacccatttc gttttggatt tttttcaatt
     2401 cctgttcttt gcttgaaaat tcaacttttt taacatgatc taagtcattc aattcttttt
     2461 ctagcttttt catctcttcg tcttttgtac cgatatctac gaatacagat acatcaacgt
     2521 ttccttctat atcctgagct aatttagttg cattcatgat aactgccatg aatacaccga
     2581 ctaaagtcaa cgtgatcgtt accgcactga ctgaagcaac tgtcatccag ccgttacgtt
     2641 tcaaactctt gatactttct aataaatggc ggaaaaaggt tctaatcatc gtatccatat
     2701 tccccttccg cttgatctcg aataatgcgg ccgttttcga tagcaattac tcggtgccga
     2761 atcgtattta cgatcgtact gttatgtgta gccatcacga cagtagttcc ttgggcattg
     2821 atccgatcta ataatttcat gatttcccat gagttttctg gatccagatt tcctgtcggt
     2881 tcatccgcga taagtacttt aggcgtattc acgattgccc gagcaataga gacacgttgc
     2941 tgttccccac cggataattc gctaggaaag actcgaactt tgtgtttcaa accaacaagg
     3001 tccagcactt ccattactcg ttttttgata tctcgaggtt ttctgccgat aacttgcata
     3061 gcgtacgcaa cattttcata tactgttttt cttggtaata atttatagtc ttggaacacc
     3121 acgccgattt ctctgcgtag ataaggaact tcgcggtttt tgattgctat tagatcatga
     3181 cctgcgacgt tcaaagttcc ttttgaagct ttttcttctc gatacatcaa tttgatgaat
     3241 gtagatttac cggctccaga cggaccaacc acatagacaa actctccttg ttcgatgaca
     3301 atcgagagat tgcggatagc ggtcgtacca tttgagtact tcttcattac atccttcatt
     3361 tcaatcatgg cgttctctcc atatcttttt cagtctttag ctattatagc atggcaatgt
     3421 ttcaacatgc ttgcaaaatc ttacaatttt atttcaattt aacaatgggt tcttattgta
     3481 gatgcatttt taaatagccg tcaataaata aatcaaggtc accgtccata actgcttgga
     3541 catttcctgt ctctacgttt gtccgatggt cttttaccat agaataggga tggaaaacat
     3601 aagaacggat ctgtgaaccc cagccgattt ctttttgttc tcctcgaagt gcagctgctt
     3661 cttgttcttt cttttccact tctaattgat aaagcttagc ttttaacatc cccatcgctt
     3721 gctcacggtt tttcaattgt gagcgttgtg cctgactagc tactactgtt cctgtaggca
     3781 agtgagtgat ccgtacagca gattctgttt tattgatgtg ctgaccacct gcaccacttg
     3841 ctcggtacgt atcgattttc aaatcatctg gattgatttc tatatcgatc gtttcatcaa
     3901 gctctggcat cacatctacc gaacaaaaag aagtatgacg tcgtttcgct gagtcaaaag
     3961 gcgaaatccg tacaaggcga tgaacacctt tttctgattt tagataacca aaagcgttat
     4021 gtcctttaat cagcagggtc acactcttga tccctgcttc atcacctgct tgataatcta
     4081 gtgtttctac ttgatagcca tgctgctctg cccaacgtgt atacatacgt aaaagcatgc
     4141 tgccccagtc ttgggactcc gttccgccag cccctggatg caattcgacg atcgcattgt
     4201 ttcgatcata aggtccgtct aacagcatag ataattcata cgtcttcatt ttttcatcta
     4261 atgcattgat acgtgtttct gcttcttctt gcattgctgc atcaggttct tcttgcagca
     4321 tctccaccat caattcgatt tcttctagtt cttcggcaag agaatggaat tggtcatagg
     4381 tttctttatt agcgttattt tcgctgatca ctttttgtgc ggcttcgctg tcatcccaaa
     4441 aaccaggttc agccattttt gcttctgctt ccgcgatgtc ttcttccagc tgatctaagt
     4501 caaagagacc tcctgaaact tgtgaccttt tcctgcattt ctgtcaggag atttcgaatt
     4561 tcggatattt ccatgttttt tccttctttc actatacttt tttcattata tctagtctaa
     4621 cagaatcagc aaataacaga aagaaggtga gacgaattct taaaaaaaga atttgtctca
     4681 ccaatcctgc tgtcagaaat tagtttgcat tttttccatg acagttctta tattttttcc
     4741 cgcttccaca aggacaagga tcatttcttc cgaccttatt cacatggatt ggctgttttt
     4801 tcgcgctagt attactattt tctttggcat ctccgtcttc tgagtgagaa gctgttcctt
     4861 gagaaacctg ttcacgctgt acgttttgac ggatctcagc tttcataaag attcgagtga
     4921 cttcatactc gatggcgcct accatatctt caaacatctt atagccttct gtttgatatt
     4981 cgactaatgg attgttctgt ccataagcac gtaagccgat tgactgacgt aattgatcca
     5041 ttgcatcaat atggtctgtc catttagagt ccacaacacg caagatcacg actttttcaa
     5101 attccaacag ttgctctgga ccatttagtt gagccgcttt ggtattaaat acttcttgtg
     5161 ctcgttcgtt tagatactct ttgatctcct gagctgtttt tccttcgata tctgcaagag
     5221 taatgctatc ttcatggaca agagtactgc cggcaaaatc gatgatactt tccaaattcc
     5281 aattttcttt ctctaattgt gtatgcgcat ctactacacg actgattgtc cgtttgacca
     5341 tgttcattag tggcttggat aagctttttt cttccatgat tacttcttgt cgctgtttat
     5401 agattacttc acgttgttca cgcatcacat catcgtattg taagacgttt ttacgtgtgt
     5461 catagttatt tccttctaca cgtttttgtg cagattctac ttgtttcgtc agcatcttgc
     5521 tttggataac agcatcttcc tcaccgatct tcatccgatc taagaaagct ttgattcgtt
     5581 ctgaaccgaa tcgtttcatc aaatcatctt ccaatgacaa gtagaactgt gacataccag
     5641 ggtctccttg acgtcctgca cgtcctcgaa gctggttatc gatacgacga gattcatgtc
     5701 gttctgtccc gataactgct agaccaccaa gttctcttac cccaagtcct aacttgatat
     5761 ctgttccccg accagccatg ttggtagcaa tcgtcacagc acctttttga ccagcattca
     5821 tgatgatctc tgcttcttta aagtgatttt tcgcattcag cacttcatga ggaatacgtt
     5881 cttgattcag catatctgat aataactcag atgtttcaac cgcaacagtt ccgactaaca
     5941 caggttgacc tttgcggtag cgatctttga tatcttgcac gacagcgtgg aacttgcttt
     6001 gtaatgttgg gtaaagcaaa tctggacgat catcacggat aaccggacga tttgtcggaa
     6061 tctggtatac ttggatgtta taaatttctc tgaattcttc ttcttctgtt ttagctgtac
     6121 cagtcattcc cgcaagtttc ttgtacatac ggaaatagtt ctggaaagtg atcgtcgcca
     6181 tggttttcgt ttcgtcttcg atctctacgc cttctttagc ttcgattgct tggtgaagtc
     6241 catctgaata acggcgtcca tccatgatcc gaccagtgaa ctggtcaaca atcagtactt
     6301 ttccgtcttg gacaacataa tcgatatcta aaagcatgat atagttggct ctcaatgctt
     6361 gatccaagtg atgggtcaat gcagtatttt caatgtcgta taagttatcc aaaccgaatg
     6421 tttcttctgc tttttcgatc cctgcttcgg ttaaactgat tgtttttgat tgtacatcga
     6481 ttttataatc ttcttcttct ttcaaacgtt tcacgaagtt gtctgctctt gtatataaag
     6541 ctgtggattt ttctgcttgc cctgaaatga tcagcggcgt tctcgcttca tcaatcaaga
     6601 ttgagtcaac ttcatcgaca atggcataat tcagcggacg ttgtaccatt tgatggcggt
     6661 aaacgaccat attgtctctc aagtagtcaa atcctaattc attattcgta ctataagtga
     6721 tatcacaatt ataagcctcg cgtttttcat ccgaagattt tgagttgata ttcaacccca
     6781 ctgttaatcc taagaaatta taaagttcgc ccatttccgt cgagtcacgg gttgctaaat
     6841 attcattgac ggtaactacg tgtacccctt cgccagtcag agcatttaga tatactggca
     6901 tcgttgcggt caaggtttta ccttcaccag tacgcatttc agggatattt ccatcgtgaa
     6961 gcacgattcc acccatcaat tgtacgtggt atggatataa acctaataca cgttttgctg
     7021 cttctcggac gactgcaaaa gcttccggaa gtaattgatc taatgtttct cctttttggt
     7081 atctgccctt gaattcagca gtttttgcct gtaattgttc atcagttaat gctgccattt
     7141 ggtcagcgta tgcttcaact ttttttgcaa tgctatctaa acgttttagt tctttcttat
     7201 cgttttcgat aagtgaacga atcaaattgg ccatgcgttg tgtaatctcc ttttccttct
     7261 gtgtattatc cagactatgt agactttact tttc
//
LOCUS       DACCXM010000126         7038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCXM010000126 DACCXM010000000
VERSION     DACCXM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.130"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            237..1094
                     /locus_tag="IVY61_002391"
     CDS             237..1094
                     /locus_tag="IVY61_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7971464.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1087..1989
                     /locus_tag="IVY61_002392"
     CDS             1087..1989
                     /locus_tag="IVY61_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7971465.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            2022..2372
                     /locus_tag="IVY61_002393"
     CDS             2022..2372
                     /locus_tag="IVY61_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7971466.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2406..3344
                     /locus_tag="IVY61_002394"
     CDS             2406..3344
                     /locus_tag="IVY61_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7971467.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3422..4315
                     /locus_tag="IVY61_002395"
     CDS             3422..4315
                     /locus_tag="IVY61_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7971468.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4353..4916
                     /gene="amaP"
                     /locus_tag="IVY61_002396"
     CDS             4353..4916
                     /gene="amaP"
                     /locus_tag="IVY61_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7971469.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4930..5118
                     /locus_tag="IVY61_002397"
     CDS             4930..5118
                     /locus_tag="IVY61_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7971470.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5131..6033
                     /locus_tag="IVY61_002398"
     CDS             5131..6033
                     /locus_tag="IVY61_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7971471.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6065..6307
                     /locus_tag="IVY61_002399"
     CDS             6065..6307
                     /locus_tag="IVY61_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7971472.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6563..6934)
                     /locus_tag="IVY61_002400"
     CDS             complement(6563..6934)
                     /locus_tag="IVY61_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971473.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2563 a         1193 c         1400 g         1882 t
ORIGIN      
        1 aattttttca aaaaacattc ttaagaaaaa ggtggctata tatagtagtt tttggtcaat
       61 acctttgaat aaaaagccat gaaaaacgaa tagttagcgc ttaccgatgc gttttgtgat
      121 aaaaaaattt taaaatattc aatgaaaaca aagcatatat tttgctttaa aaccaaatga
      181 ggaatattct agatgtagaa caggttagaa aaagattaga aaaaggagtg attttgatgg
      241 agaagagata cggcgtagtt cctgtggtaa ctgcttcaga cgaaaattat gctccttact
      301 tgagcgtgat gatcgcaact gcattagaaa attgcaataa aacaagacgc ataaaatttt
      361 atgtgattga cgatggtcta tcagaatata gtaaagaagg attggaagaa acggtaaata
      421 aatattcaag taatgcaagt atccaattcc tgacagtgga aaaagatatc tacgaagact
      481 tcctagtcag tgaccatatc acaactaccg cttacttaag aatttcttta ccaaacttat
      541 tagctaaaga ggattataag aaagtccttt acttagattc agatgtgtta gtactagacg
      601 atatcgtaaa actatatgat gagccgttaa atggaaaaac aatcggcgct attatcgatc
      661 caggccaagt gaaagctttg gaaagattag gaatcgattc cgatgacttg tacttcaatt
      721 caggagtgat ggtgattgac attgatcaat ggaataaaaa agaaattaca gaaaaaacta
      781 tccattacct cagtgaaaat ggcgatcgaa tcatctatca cgaccaagat gctttgaatg
      841 ctgtacttta tgaagattgg gaacaacttc atccaaaatg gaacatgcag acttcgctga
      901 tttttgaacg tcatccagca ccaaatgaaa aatatgaacg tcagtataaa gaaggaaatg
      961 aaaaaccttc gatcgttcac tttacaggac atgataaacc ttggaatacg ttgaaagatc
     1021 atccgtatac caatttatac ttaaaaaaat tagcacacag tactctaacg aaagtaggcg
     1081 agatcaatga ataaaaaaga aatagcagta gtcgcaagtt gtaacacaaa attcgtacca
     1141 catttagctg cattatttgt ctcagtatta gataattgca acccgtctaa atttgttcgg
     1201 ttctatgtga ttgacgatga tatcgatttt gaaagtaaac agcttttacg cttctcagtg
     1261 aaaaacgcac gaatgaactc agatgtggaa ttcttgaaaa tcaataaaga attctttaca
     1321 aatgtggtaa tcagcgatcg tattccagaa acagcttatt atcgtattgc gattccggaa
     1381 ttgttccgcg gcacagaagt tgaacgtatc ttatatatgg attgcgacat gatcgcttta
     1441 caagatatct ctaaattatg gcgtcttgac ttcggtgatt caatcgtagc agctgttgaa
     1501 gacgctgggt tccatcaacg actggaaaaa atgaaaattt cagctaaatc aatgcgttat
     1561 ttcaactcag gtttgatgct gatcaatgtg aaaaaatggt tagatgaaaa tattacgcaa
     1621 aaagtattag atttcatcga acataatcca gaaaaattac gattccatga ccaagatgca
     1681 ttaaatgcta tcttacatga ccgttggtta ccacttcatc cgcgttggaa tgcacaaggc
     1741 tatatcatgg caaaagcaaa aaaacatcca acagcagcag gtgaacgtga atacgaagaa
     1801 actcgcaaca atccttacat tatccatttt tcaggacatg tgaaaccgtg gagcaaggat
     1861 tttgaagggc caacgaagaa atattacgaa aaatatgcag ggatgacggc tttccgttgt
     1921 gtagctaaat tccctaaata tccaaaatat gcaaaagttc aaccaaaaac agatgtagtt
     1981 gttaaataat agaagaccac aactgaagag ggggagtagg aatggaaatt acaatgtcta
     2041 aaaatgcagt agagacgtta attgaaaagg ttggggaaaa tacaaaaatc gcactcgcgc
     2101 ttatcaatga ttccgatcca tttctaaggg acaaaggtgc ttttgcaaaa ggcagctttt
     2161 ttcaaatcat tccttttgtt tcggaatttg gagaatatgc gactaaaatc gagcatccat
     2221 tattggacat ctatacatct aaattagagc aaaattattt cggtaaacga ttgaacatgg
     2281 acttcaacaa acagcttgac agcttttcat tagaaaatga aatcgcagta ctcgattata
     2341 atatcaaatt aaagaactgc ttcttcagct aatagtttcg agaaagtcca aaaaggaggt
     2401 atacattgag atactacttt gtggatgaag aaaagaatgt aatagaaaca gatgaaaaaa
     2461 aatataattg gctcgtactt gatagtgcaa agcccgaaga gattgagcaa gcaatcgaag
     2521 aattcgagct tcctgatgat attttcgtag gaaccgaata tccggaagaa gtttctcgga
     2581 tggagcgcct ttatgagacg aaactagagc atccattttc tttggtagtg attaatctag
     2641 atcagtcaga taatcgtatc gaaaaaaaat taacgccaat atcttttgta ctttccgata
     2701 atctcttgat tacttgtatc gatagaaaaa ccgaatttgt ggatcggctg ctgacaaagc
     2761 atggaaatca tttaaactcg tttgaaaaga tcatcacgta tacattatta gatatttaca
     2821 cccattacgt gaaagaacta agagagatga aaaagcgaat tgacgctttg gataaagcag
     2881 ctcgtaaaac aacggaaaat gaagaactgt ataaacaggc agatcttgaa agagacatcg
     2941 tatatatcga tcatacctta agagatcaac gggaaacaat ggatcaatta tggaattctg
     3001 aaactttcat caaacgttta gacgatgaac agctactcta tgatgtccat ttgagacagc
     3061 gacaaacaga aaaaatgatc gagatctatc gagatttatt agagagtatt ggtgatctat
     3121 tcaacgggat gatggacaat aatctaaatc atatcatgaa atatctagat tcagctgcat
     3181 taatcatttc cgttccggcg ttgttcgcag ggatttgggg aatgaataca ggaggattac
     3241 cgggtaaaag ttctgctcta ggctttatac tcgtagtctt tggctctttg atagcagcag
     3301 tagttttagg ttaccattta taccggaaag attatacaaa ttaaagaatc tatttaaaac
     3361 aggtatcaaa caaagaaatt tgttttggca atacaattta aaaactagga ggaatacagc
     3421 aatgacagaa ccaaaattaa tcgacccgcg aaacttgtac catacggaga aatttcctaa
     3481 acaagatcag gatacaccag ctttacagca gaatatgatt cctgttcctg attgtggaga
     3541 agaatcctat gaaggcaata atcaactaga aaaccgccgt gtactaatca ctggtggaga
     3601 ttcagggatc ggtcgtgcag cagcgattgc atttgctaga gaaggggcag atatcgctct
     3661 tcaattcttc ccaggagaag aaaaagatgc agaggaagta gcaaaatacg tgaaagatgc
     3721 aggacgtaag attgtcttac taccttttga tcttcgtgac gaacaagcac ctaaagaaat
     3781 catagataaa gccgtgagtg aacttggcgg gctagatacg atggtactaa acgcaggaca
     3841 gcaaatttct tgtcagtcta ttgctgagtt accacttgag caagtcaaag acacatttat
     3901 gataaacatc atcgctatgt ttgctttagt gaaagaagct gttccgcata tcccagcagg
     3961 aggagcaatc gtaacaacaa cttctgttca agcattcaat ccaagcgaac atttattaga
     4021 ttacgcagca acaaaagctt caatcgctaa ctttacgatc ggtttagcaa aacagcttgc
     4081 acctaaagga atccgtgtaa atggtgtagc acctggtcca atctggacac cattacaact
     4141 agacgaagga caaccttctg attcgatccc tgaatttggt cagcaaagtt tactagaacg
     4201 tgcaggtcag ccaggagaat tagcaccagt ttatgttttc ttagcatcga ataaagcaag
     4261 ttatgttaca gcccaaattt acggagtaac tggtggagaa gcgatcaact tataagaagt
     4321 tgatcgtttc acccctatga ggaggtgcga aaatgaataa aggtgtaaaa acgatcggag
     4381 tgatcggaac attacttttg ctatctgtgc ttttgtttgc gatactatta agtgcaacct
     4441 atccattgcc ttttcagcta gagaagtttc gattcttttc catcactaat tcatacgtgc
     4501 aacaatatgt attttggctt gccgttgcat ttgctatttt gacaattatc acgttacttg
     4561 ttttcctgtt ttatccaaaa acaatcggaa actttctact gaaacatggt gatggggagt
     4621 tgacgttgga taaaaaagca attgaaggaa tggttcgttc tcacttgcat gaagatgaat
     4681 tcatccattc accaaaagta aatgtcaaag caacgaaaaa caagatcgcc gtcaatgtaa
     4741 aaggggagtt gaaacgaact tcagcactca ttggtaaaac aggaacattg atgaaagaaa
     4801 tcgaaaatga agtacaacac atattgggaa caaaagagcc agtgaaagta tcggtgaaat
     4861 acacaggctt tgaagaacca gctaaacgaa gcaatgaccg caaacgagta gaataggggg
     4921 ggaccaaaaa tgaaagaact attttcgaca tacaagctcc ctatcatctt tggggtactt
     4981 ggattgatat tagccgtttt atggatcacc gtgggctttt tcaagacctt attgatcttg
     5041 atcatggcag cgatcggcgt cgctatcggt ttttatctga aacaaacgaa aatcttagat
     5101 cactatttta aatcttagga ggaaacatcc atgaataaca ataacaaaaa taataacaat
     5161 aaaatgaaca accaaaaacc agtgtctgca catccagacc caacgacacc aaaccaatca
     5221 aatggtgtga acgatgaaaa gaacaaaaca gcaccagtat ctgcacatcc agatccagta
     5281 gtccctaacg ttcaacaagg agtagaagag aagaaacatg atgcagaaaa gcatgcacct
     5341 catcatccag gaactccaga atctaaagtt ccagaacaac atgatgcaat cgatccttct
     5401 aaacctgtgg aagagcaaaa acatccttct gaccatgcga aagcacaaga aaaacttagt
     5461 tctgaccaca caaacggaca aggaaaactt ggttctgatc atacaaataa ccaaggaaag
     5521 catgattctg aacaggaaga ttcaatgcgc ggtgagctga attatgaaga taaagttgtg
     5581 caaaaaatca tcggaatcgc aattgaacaa gtagacggtt tattatctgc aaacggtggt
     5641 ttcttctcta atgtggcagg aaaacttgtg aatacagata atgtaacggc tgggatcgaa
     5701 acagaagtcg gtaaaaaaca agtagcagtt gatatggatg tcattgtgga atatggcaaa
     5761 gatatcgaaa aaatctttga agaaatgcaa gaagtaatcg gcaaagaagt aaaaaatatg
     5821 acacatttag aagtgatcga agtcaacgct aacgtagtgg acatcaaaac aaaagaagaa
     5881 tttgaaaaag atcaagaaac ggtgcaagac aaagtaactg acgcagcgaa aaaaaccggt
     5941 gaatttgctt cgaatcaaac agataaagta aaatcagcag ctggaagcgg cgcacaaaaa
     6001 gtaaaagaaa atacagaacc acgggttcag taataaggaa gtaaaacatt aggaggaacg
     6061 aaacatggga tttatttggt cacttatcgt cggaggaatt atcggagcaa ttgcaggagc
     6121 aatcacgaat aaaggttctt cgatggggat tattgcaaat gtcgtagcag gtttagttgg
     6181 atcagcactt ggtcaagcat tattaggttc ttggggacca agtattgcag gaatggcaat
     6241 cgtgccatca ttgattggtg ccatcatttt agtactaatc gtatcattct ttgctcgaaa
     6301 actgtaaaaa gacttttaaa aagaggtcgt gaaaaagctg ttcttcatca agtaataaga
     6361 acagccaaac aaaaatagct tctcatattt ttcatttggt tgggcatatt atcgaagatg
     6421 caagcttttg aacactgttt atacggaata agaggctgtg acaagacttt tgtcacagcc
     6481 tcttatttat gcgggcaaat tcccaattag atttttaaaa gtttttcctt aaatgtgtct
     6541 ttattgatac agagaagctt ctttaataaa acaatgcctc cctaaatttt cttgagatag
     6601 gaaaggtgaa catacaaaat aatacgacca ctttgaaaac ccagatgatc cacaaatgga
     6661 tcgtatgata ctgatgaagt aaaaagcaat ggatcagaaa ctcaaagaga ttgaaaaaga
     6721 caaggaagaa ataatatctc ttttttactc ttgtctgttt tagccaataa tagctcgtaa
     6781 agtaatctag acaaacaaat agaagtaaac aaagtttcgt aagagcagca atcgtatcat
     6841 gttgctctgc tagacgattg acgagcagga aaaatgttgg taatggaata tagatcacga
     6901 gtaatgaaag aaaaaagtaa acaataaatg gcatactatt cctcctaatt gtgtgagttc
     6961 gccctcattt taaaataacg atatttgttt gtgaagcttt ttcgtgtcgt aacaaatgtt
     7021 atctaatgta tttttaaa
//
LOCUS       DACCXM010000127         6875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCXM010000127 DACCXM010000000
VERSION     DACCXM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.131"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(179..1951)
                     /locus_tag="IVY61_002401"
     CDS             complement(179..1951)
                     /locus_tag="IVY61_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7971474.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1905..3659)
                     /locus_tag="IVY61_002402"
     CDS             complement(1905..3659)
                     /locus_tag="IVY61_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7971475.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3769..4338)
                     /locus_tag="IVY61_002403"
     CDS             complement(3769..4338)
                     /locus_tag="IVY61_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP7971476.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4356..5780)
                     /locus_tag="IVY61_002404"
     CDS             complement(4356..5780)
                     /locus_tag="IVY61_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7971477.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(5782..6450)
                     /locus_tag="IVY61_002405"
     CDS             complement(5782..6450)
                     /locus_tag="IVY61_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7971478.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6580..>6875)
                     /locus_tag="IVY61_002406"
     CDS             complement(6580..>6875)
                     /locus_tag="IVY61_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7971479.1"
                     /translation="IFDLILFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQ
                     NLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT         2346 a         1247 c          920 g         2362 t
ORIGIN      
        1 aattttttaa aactttgatt attccttata ttataagaat agacattaag tgaactaatt
       61 caatcatact gattctttcg tgtattcaaa attagatgct gtttaataaa attattcttt
      121 ttgaaaaaga attataacta tatcccaaaa attgtaggtt aatttaaagc ctatttcatc
      181 atcgtgattg agcttctttt tcatagacga atttctcaga tagtttagtt ttctttatga
      241 gatttttgta tatttctgac cttcgataca actcattatg agtaccttga tcaactagtt
      301 ttccttgatc catcactaca attttattta ccttttcaat agacttcaag cgatgcgata
      361 taattagtac tgtcttatct tgtattaaac gatttaaact ttcctgaata cgtttttcat
      421 tttccgcatc taaactggca gtaatttcat ctaaaattaa aattggcgca tttttcaaaa
      481 aagcccgtgc tatggatagg cgttgtcgct ctcctccaga aagtgaactt ccgttttctc
      541 cgaccaatgt atgataaccg tcaggtagtt tttcaataaa gtcttcgcag ttagctaaac
      601 gagctgcttt tttcacttcc tcatctgacg cttgactatt tccaatacgt atattttcca
      661 aaatacttgt attaaacaaa gttacctctt gaaaaacaat agcgattttg ctaaacaaag
      721 aagctggaga tacacgttta atatcatacc catcaattaa aatacagcct tcatcataat
      781 catacagtct agacactaat ttcaagatac tagttttccc ggaaccacta gcacctacta
      841 acgcagtcac ttctccttgt ttagccgtaa aagagatatg atctaaaatt ggggtattgt
      901 tatcatatga gaaagagaca tctcgcaact caatgtcaaa agatttcaga ggactatctg
      961 atccctcttg gatactggtt tccttcatag ctctaattcg ctgtatttta ggagctaaat
     1021 aaaatatttc aagcactgct tctttcatag aatcgaagga atcctttatt tttattgcgg
     1081 ctagtaagta accaacgacg tagagtatag tcacttcacc cgttaacagt aagtgaacac
     1141 ctactagaat gactactgct aaactaacat atgagaaaat agaggataag gccatgactg
     1201 aaaaagtact taattctacc tttaaatgaa tcctttcact ctcttccatt tttttgaaaa
     1261 gtctgtcttg aactttttta gatagattaa agctattaat ctcttgctgc aattctatag
     1321 tttcttgaaa ttcttccgag ttctctctca aagtatcgta atatttagta ttggctttcg
     1381 tttgggattt ctttgataac aagattaaga caaaactaaa taacgttggc aaaataacag
     1441 ctaatcccat tttgacatta ccaaccaaaa gcatcactga aataaaaggg aaaaacagag
     1501 ccataccgcc ggctttaggt attgcatgac tcatcgcatg ctcaatacct tcaacgtcag
     1561 acatgatagt ttgagataaa tctgataaat tatgttttga aaaataggat aatggtaaat
     1621 ttgacaaatc gtctgcaatt tgcactctta aatgcgcact ttctttataa gtactattat
     1681 ataagttttc atactcccta tctaacaacc aatacatgaa aagcaaagta acaaacgata
     1741 cggcaaaata aagccaacgg ggctttgtat tttctaaaac taattcgtct atcagcatca
     1801 ttataatgaa tgctggtacc atattaatgc agtatacaaa aaaactagaa atagaagctt
     1861 tagttaaagc ttctgcgccc tgatcactta aagcaaattt gtttttataa aaacttttca
     1921 ttgtcaagcc tccaatcatt tgcattctca taattagtga ccaattgttt atacacttga
     1981 tgtttagcca ttaattcatt atgatttcca cgttctacta ttttcccttc tgacaagaca
     2041 agaatttcat ctacagattt aatgctggat aatcgatgag ctatcataat aactgttttt
     2101 tctttcatca gatgttgaaa agccttttgt agttcatact cattatctgg atcaatagat
     2161 gcactggctt catccataat aacaatcttt gcatctttta aaattgctct ggcaatagct
     2221 atacgttgtt tttctcctcc tgacagataa actcctttac taccaattaa ggtatgctct
     2281 ctttcgggaa atttttctaa aatcgattcg caaccggcta atcttagcgc gtcaaatact
     2341 ttacttgctg gcgcttgagg atttgctata gaaacgttat cgtaaatact ctgattgaat
     2401 agtttcgtat cttgaaagac aaaagcaatt gtgttaataa ttgtgtcttt tgaatagtga
     2461 tttaaaggct taccaccaat tttaatctgt cccttatcca cattgtagaa acctgaaatt
     2521 agtttagcta tcgtactctt tccgcttcct gattttccta ctaatgcata aattcttttt
     2581 tgttctaaaa ctaagttgaa attttctaaa acacactctt ctttgtatga aaagctgaca
     2641 ttttcaaatg caatgtcaca atttttaatc tgtgtctcgt tcccatattt tagttggtct
     2701 tgcttcattt ctaaataaag ggagtctagt gtttcgactg catatcttgc ttcaaacaag
     2761 tgcatactta catacattat tctcatgaaa gaaacaaaaa taattccact taaaaaaaac
     2821 aacatcaaca agtcaacagc taatatcttg ggagtggtaa gagaagttat aaaaaaggtt
     2881 ataggaacta tcaaaatgca aactatgcca aaaaatagcc attgaaaaag aacaaaaggt
     2941 gttttcccac tcaaagaata cttatatgca aacttagcat aatcgttaat tgctttattc
     3001 aaggctttaa atgatgttac tttagcacca aatatcttta taacttgaat tccgcggata
     3061 tattctaccg tttcagagct taacacatct aacgattctt gatatttttg aataaaattt
     3121 gtattctctg tcattttctt taacaagaat acaccaatta ctaacatggc gattagaacc
     3181 aatccaattt taaagttaat gaaaaagcct aaggcaactg ataaaacagg tgttactatg
     3241 gcttgagcac tatctggaat caagtgagca attgccatat gtgttttagc cgcgttgtca
     3301 tcaattgtct ttcgaatata tcccgaagaa tttaaatcaa aaaaacgaaa acttgcttct
     3361 gttaaaccgc taattccctt ttttctcaaa ttagtctcta agcgaaaccc aaggatatga
     3421 gaaaataagc cagaaaaaat ataaaataaa gctcctaaag ttaaccaagc aacagtttga
     3481 accgcataac tcttagctaa atattcatct cctacaataa ttagagactg aagaaatttg
     3541 taaatactgt aatatccata gactgttaat acgacagaaa aatcagaaaa taaagtggct
     3601 aaccatccgt agtatttcat tttagggaca taactaaata attttttgta tactttcatt
     3661 ttttcctccc ccgcattcaa tttcttctct tattcgttta caactgttaa tgctttccgt
     3721 aatagcgtaa attagagatg tacaaacaga taaaaggtaa actacgttct attttttgta
     3781 tttttttaag acttttcttc caatcaatac accaatcata gataaacttg ctacaactaa
     3841 cactgaaatg attccccata tacctaccgc atagtggatc attgcatcca ttgttgcagc
     3901 agattgtcct ttatctatcc aatcttgacg aaaacgatct gtaaagaacc atataggaat
     3961 ccactgtccg gcagcactgc ctaattgcat tagagcccaa gacgcaactt gaggaattgc
     4021 tcgatcatat ccaaatacag atagaataat atcagctaag atcgccatac taatcgaact
     4081 gataaaccaa ggtaagtacg ccccgcccat taacgtaaaa aataacaata aaacaattgt
     4141 gtaaatagta aaaattcctt ttctagggta gttatagcta atataaacaa atatgattcc
     4201 agataataat ccaaaaatcc cgggggatat gctgtgacca aacgccccac caatactact
     4261 aaccattatt gaaaaaaagt aaattactag ggaaataact gtagctaaac caataaaaat
     4321 ataatctttt gttttcatat atattctcct caattctatt tcgttatgtc attaaaaatc
     4381 tgttctatca aagttgaatc ttctactcta agttttatat ctttttttat tccaccattt
     4441 tttaaataaa taattctatt gcatgtttta taaagaaatt ccggatcatg agtagctata
     4501 actactgcac ccttttcttt taagtcttta ataaggtcag caatcctttc catattaata
     4561 taatccaacc cacttgtagg ttcatcaaat agaaataaat tagtttttga caatgaagct
     4621 atagaaacca ataatctttg tttttctcca ccggataaat caaacggtaa ttgatctttc
     4681 attttataaa gttccatttt ttttaaaatt ttttcttgct gatctttact aactatttca
     4741 ttccccaggg ctaactcgct cctcactgat tctgtgaaaa attggtaatc catttcttgc
     4801 attactaaaa acggcgatga caaatttttt ttattttcga ttatctttcc tttggtaggg
     4861 cgaataatct tcatgagtat tcttagtaag gttgttttcc ccacaccgtt tttccctacc
     4921 aaaccaataa catctccaac tttgacatca aaatttatgt cttttagaat attttcataa
     4981 gtaatttctt caacttttaa tacatctgca cttacctttt cgatataggg aacttcaatc
     5041 gaactattaa aaatgtcaaa tttccttaat gcgttattgt tttcagtttt taacaactga
     5101 atagttgtag tttttacagt tctattttta acaaatagaa cttgatcaat taattctttc
     5161 aaatagtaaa aacgatgttc agctataaca atagtaaatc ctttattttt taaatcacaa
     5221 agtaagtcag caagtttctt agtcgctttg aagtccaaat ttgaagacgg ttcatccaat
     5281 ataattaatt tagagtttag cgataaacta catccaatag aaattttctg ccgttcacca
     5341 cttgatagcg aaaaaatgtt tttatctaat atatttgaaa gatctaataa ttcagctaat
     5401 tcattgattc ttttatccat atgctctttt gtaaatccaa agttttccat cgaaaatact
     5461 aactcactcg ttgtatttgt tgtaaagaat tgtcctctag gatcttgact tacgtagcca
     5521 atattttttg atatactttc ttgtgttaag gattgtagcg agttattttc aaataaaatt
     5581 tccccaacca attttccgtc ataaagtctt ggaataagtc cattcattaa ctttaatagt
     5641 gtggatttcc cactcccaga agaaccagta atcaatgtaa gactgccttt ttttattgaa
     5701 aaattaaggt caacaagcgc ttgttcctta acatccttat atgaaaaaga aacattgtta
     5761 aattcaaaca attctttcac ctcaaatcaa aatacttaag aaaaaaatag taacggaaaa
     5821 caggatgata tagtctaata agctcatgtt tgttttatag taagaacttc ttttcacagg
     5881 agattctatc ccctttgtta ttgcattagc ggtcatttct tcagcaatca tagttgcacg
     5941 tataagaacg ggaactaaca gaaattccaa atattgcaga ggctgaaaaa aagacaagcg
     6001 aatgtttctc atttttaaag attctttaat cattcttatt tcagaacgat aagtcggaaa
     6061 gaatctgatc actactgtaa tacttaacat tatttttttc ggtaaatgta ttttttctaa
     6121 agaggataat aattcattgc tagtatagac actaaccaac aaccatgaga tcattacgac
     6181 cggcaacatt cgaaatacaa tatataagga tgtcgcaaaa aatatagtaa aaaactctat
     6241 cttgatagca tcaataattt tcattactaa aaacagaact gaataaatca agatgaaata
     6301 aatacatgtc tttattttac gaaacatcaa tactaaaact gtagcaataa tgaaacaccc
     6361 cacttgaaat atatttccct ttcctaaaaa aataatgacc ggaaaaaaaa tatttaaagt
     6421 aatcaaggtt aacggattga tttttgacac tctttcacct ccaaaaaata agatcgcact
     6481 gttatcttaa gctgataaaa atgcaccgtt attcagataa cagtgcgatc ttatcaaaaa
     6541 gcataactga gaacaattct caatgcaagc tttttttaat taactcagta attcaatcca
     6601 cccaggaaca gaaaattgat acaatcttct ggttaatagc atagtctttt cttttgaata
     6661 gctatgcttc aatatatcat agaatgtagc gtagaatgag taaatgtaga agtggatttc
     6721 ttcttcatta aagactaaat tttgattgat cattccgata gtctttaact tatctagaca
     6781 acagactgtc tttttcgtca caaatgatgt aattttttca acaatatctt gatatttggt
     6841 tccagctgat tggaataaaa tcaaatcgaa tatac
//
LOCUS       DACCXM010000128         6783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCXM010000128 DACCXM010000000
VERSION     DACCXM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.132"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(27..860)
                     /locus_tag="IVY61_002407"
     CDS             complement(27..860)
                     /locus_tag="IVY61_002407"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP7971480.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(1052..1756)
                     /locus_tag="IVY61_002408"
     CDS             complement(1052..1756)
                     /locus_tag="IVY61_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7971481.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(1779..2405)
                     /locus_tag="IVY61_002409"
     CDS             complement(1779..2405)
                     /locus_tag="IVY61_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7971482.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            2731..5106
                     /locus_tag="IVY61_002410"
     CDS             2731..5106
                     /locus_tag="IVY61_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP7971483.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(5130..5621)
                     /locus_tag="IVY61_002411"
     CDS             complement(5130..5621)
                     /locus_tag="IVY61_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP7971484.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            5808..6728
                     /locus_tag="IVY61_002412"
     CDS             5808..6728
                     /locus_tag="IVY61_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7971485.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT         2185 a         1238 c         1313 g         2047 t
ORIGIN      
        1 aaataagaga agtgttctgg ttttttctac attgcaataa accaataacc aatcaaaatg
       61 ataccaagga taatacgata ccagccaaaa gcggtaaagt cgtttttctt caaatagctt
      121 agtaagaatt tgatagctaa gacggaaacc acaaaagata cgaatgttcc tacaagaaga
      181 acaacagttt cttccatttt gaatgaattc ccttcagcta aatatttgac gatcttcaag
      241 ccgcttgcgc caaacatgac aggaatccct aagaaaaatg aaaactctgt agcgacaaaa
      301 cgcgaagcac caatcaagat agatcctagg atcgttgcgc cagaacggga tgttccggga
      361 acaagtgcca agacttggaa taacccaaca agaagagctg cttggtacgt aaagtcattc
      421 agagaagtcc aacgaggtgt tttgtttttg ttgcgttttt cgacaataat aaaggcaata
      481 ccgtaaacga tcaaaacaat ggcaactggt aagaacttgt ggaaatttgc ttctaaccaa
      541 tcatccaaag gcaaaccaat gatgatggct ggtatgcaag caacgatcac tttaaaccaa
      601 agtgtccacg tatctttttt ttctaccgtt gatttacttg gagaaaatgg gtttaacttg
      661 tgaaaataaa gaacgacgac agccaagatc gcccctaatt ggataacgac attgaacatc
      721 gacataaatt gtgaactggc atccagtttg atgaattcat ctgctaggat caaatgacca
      781 gtactactga tgggcagcca ttccgttatt ccttcaataa taccgagaat cactgctttg
      841 ataatatttg caaaaaacat aaattccttc cttcccaatt aagatacatc ttatagtata
      901 ccgttatttt ttgagaaacc caatctgttt tttaatatta agaaaaactt gagaaataaa
      961 gcagataagt acacgaagag aaaaaagaag acaaagccct cgttcagact ttgcctttta
     1021 tgattctctg gtaaaaatta aaatatcccc attatagaaa cgagtcgcga ctatgtgttt
     1081 tcccgttatc gatataagac gtatcattga tgccgtggat agaaaatcga ccgtttgcat
     1141 aagtgacctt tgttacgctg ccatttaaaa gcccagcacg aacaggctgt ttaggatcga
     1201 tcagattaag caaaaaggcg atcgtcaaac catgagatac gactaaaaca tttccaccac
     1261 catttttttc gcagtgatgg gcgatatctt caaatcctgt ccatacacgg tctcgcaatg
     1321 tttcataagt ttccgcccac ccagcggtat ctaagttgta tacagcatta gcaatttgtt
     1381 caaaagaaac ttctgtggta aacatttcat catatgtttt aaaattcaat acacgaggga
     1441 tcacacccca catttccatg tcatatccac cttcaagaga cccgaaacac cattctctaa
     1501 tccttttttc aataaaataa ggaatttctt ttcccttaga atgttctcct agaataatac
     1561 gggcagtttc gattgcacga ccactgtcac tagaataagc aaatgaaaag tcgacatttt
     1621 ttaacccgcg acctaaataa tgaatgcctt cttgtccttg ctttgtgagg ggtgtatcac
     1681 accagccttg tactcgttca attgtattga acatcgtttt tccgtgtcgt acgatataaa
     1741 gtgtagtttc tgtcatttta ttctcctcct aagtcgtttt aacgattgaa aaaaggattt
     1801 gtcctttttt cctgttcaat cgtcgttgca tcgccatgtc ctggatagac tgcaaattca
     1861 tcaggtaata caaacagctg ggtccgaata ctatgaagta attgttctaa attccctgta
     1921 tacagatcgg ttctaccgat acttcctcta aacagggcgt cgccagaaac gacaaaatca
     1981 tcaaaaataa agctgacgct tcctatagaa tgccctgggg tagggacgac gcgaaaagac
     2041 atgtttccta aagtataagt tttcatttca aattcatatt ctgcaggcga gacaataaca
     2101 tcagcaatat catcatgtct accgagtcca gaaagattca aaatcggatc acctagccat
     2161 ttttgctcta gtgggctgac ataaactggg atttgataat acgcgcgtaa aggttccact
     2221 gcaccgatat gatcgtagtg agtatgggta agcaaaatcg ctaccggctg ttggtttgtt
     2281 tttttgattt cttgtttgat tcgttccgca tcttctccag ggtcaatgat caaaagtgct
     2341 tcttcgttgt agaccaaata acagttttct tcaatcattc ctgtttttaa tctttcgata
     2401 tgcatgtgca ttcctccttg atagccaaaa cttctttcat ccattagtat aacgcattca
     2461 ttgctaggaa acaaaaacag gtacttattt ctctttactt atctgaatta tcagactata
     2521 tgaaaagtat ttggatattc taaagagaag gaaagtaagt tttcatagca ttttaatatg
     2581 tccttgttac attagattca tccaaaaatc caaattctat atcctatatc cctgcctaat
     2641 cagcagggtg tttttttatg tttggaaaga gagctctttt tgctaaacta ggttttttca
     2701 ggtaatctta gagagaaaga ggtggtggaa atgatcaaac agtcaaaaat atctgtgcgg
     2761 cacttagtag aatttatttt gcgacgtgga agtatcgaca atcgtaaaaa aagtaaccat
     2821 actgcattag aaggagcgag aatccaccgc aagctccaaa aagatgcggg agaaaactat
     2881 gaaaaagaag tctttttaaa aacaacagtt caattagatg aatattacct gatcgttgaa
     2941 ggccgtgcag acggtatttt caaaagagac ggtatttatt atatagatga aatcaaaacc
     3001 tccgaaccac gatttgaaga tttagaacca gaacaagtgg aacttttctt tcatcaagca
     3061 agggtgtatg cctatatcta tagtcaagaa catgatttac aagaaataaa tctgcaattg
     3121 acttacttcc agacggcaga agaagtgatc acgagaaagg ttgaacatca gacaagagag
     3181 cagttagaaa ctttttttaa aaagctgact gaagattata aagaatggct tgtttttcaa
     3241 gaaaactggc gcactgtacg caatgcctct ttgatggctt tgaaattccc ccatgatgag
     3301 tataggaaag gtcagcgtga actagcggta gctgcttata aaacgatccg tacgaaacag
     3361 aaattgtttg tagaagcacc taccggaaca ggaaaaacaa tctctacgtt attccccgct
     3421 ttgaaagctg taggggaaga agagggagaa aagatctttt atctaacggc taaaacaata
     3481 actagacaag tagctgaaga tgctatgaca gcattaaaag atacaggagc tgaagtaaaa
     3541 agtgtcactt tgacggcgaa agacaagatc tgctttttag atgagacgac ctgtaatcca
     3601 gatcagtgcc catatgccaa tggttattac aatcggatta atgaaggttt atgggatctt
     3661 ttgaatcatg agaatcagat cactcgtgaa gtcatcgaaa cttatgcaag aaagcataca
     3721 ctttgtcctt tcgaactatc attagatgtc agtatctggt gtgacgtaat cattggggat
     3781 tacaattatt tatttgatcc aactgtttat ttaagacgtt tttttgaaga tgaaaaaaac
     3841 gaggactatc tgtttttgat tgacgaagca cataatttgg tgaatcgttc ccgagagatg
     3901 tattcggcag aactatctta tgaaaaaacg aaacgaagta aggaagctat accaaaggaa
     3961 ttcaaaaaac ttcatcgccg tttcaacaaa ctattaaaag aatttgattc gatccgagaa
     4021 atagcaaaag aagatcattg ggattatcat catcaaaaag caccggctga atctttagta
     4081 aaggcaggtt atcaattaag cgaaaaaatt aaagaatggc tggcagaatt tcctgagcac
     4141 ccatcacaag aacaattgct tccttattat tttgatttgc ttcatttttt gaaggtcagt
     4201 gaattttatg atgatcacta cgaaacaaca gtcgaaaaaa cgtatcgcga tctgatagta
     4261 aaagagtttt gtattgatcc aagcttgttt cttgagcagt cgttggataa aggaaaaagc
     4321 agtattcttt tttctgctag tttttcacca ctctcttatt atcaggaaac actgggggga
     4381 caaaaaagtc tggcttacaa actgccgagt cctttcccag aaggaaacca gcaagttttc
     4441 atcgcaaatt acctagagac gacttatcga aaaagggaac acagtctggc aaaattagta
     4501 gagacgattc atgcttttgc tgatggaaag gtcgggaatt atttcgtttt ctttccttct
     4561 tatcaatatt tagatcttgc ggtggaagca ttcagacagc aatatcccga cagcaatgtg
     4621 ctaatccaag aaacggacat gaatgaggaa gaacgcgaac gatttttaac aaaatttcaa
     4681 acaaatcccg ctgaaacatt gattggattt tgcgtacttg gcgggatttt ttcagaagga
     4741 atcgatttga gaggggaccg attgatcggc agtatgatcg taggtgtagg tttgccgcag
     4801 atgaatcatg agcaggaact gatcaaatca tattatgatg agaaggaaca ccttggattt
     4861 gcttacgctt atcagcttcc tggaatgaac aaagtcttgc aagctggtgg tcgagtgatt
     4921 cgtggcatgg cggatcaagg aatcgttgta ttggcagatc aacgattttc tagtttttct
     4981 tatcgacgtt tattcccaca acattggcaa acagcaagag aagtacgaag tgttggggaa
     5041 ttggaagaag gaatacagcg tttttggtta tcaaaagaag cagtaaatca aaaaaacgag
     5101 gaatgacaga cgtcagcctc gttttttgat taacgatagt tagtgaattg caactcgatt
     5161 ggtaagtcaa gtttacgtaa taaagcaatc acagcttgta gatcatcacg gttcttacct
     5221 gtcactctaa tctgatcttc ttggatttgt gcttttactt tcagtttttc atttttgata
     5281 gctgtagtga ttttcttcgc gttttcacga tcgattccgt tgaccaaatc ggcagtttgt
     5341 cgagctttgc cacctagagc gtgttctgat tcggaaaaat ggatattttt gattggcaca
     5401 tttcttttga tcagcttacc aaataataca tctttgatct gttcgatttt gaaatcatca
     5461 tcgctcaata caactagatt gctgttctct aatttgattt ctactgtaga atccttgaag
     5521 tcaaagcgat tggacaattc ttttgttgtt tgctgaatcg cattttttac ttcttctgta
     5581 ttgacttctg atacaatgtc gaagctagca tcttttgcca taggaaccct cctgatatac
     5641 atataaatat ttaccataca tctatcttat cataggatta gggcagattc ttacctgttt
     5701 gctaaaattc ttctcatttg agttcgaagt attttatttt aatagataaa ctgttattct
     5761 tacatttgga ataccaaagt aggggagagg tgcttgtgag taaaattatg actgcgtatc
     5821 ataaaaatga aagattaaag aaaatagtgg ttattttgat aaccggattg accgctgcgg
     5881 tcgctttaaa ttattttttg attccagcca atgtcttttc tgcagggatg aatgggatcg
     5941 ctcaaatcat ttcctcgctt ttatatgaat ggtttcatat caagatagac acaggaacat
     6001 ttatttttct tttgaatatc cctgtgtttg tattagggtt tctcaaagta ggaaaacgag
     6061 caacgatcct tagttttgtg aatgttgtat gtgtatcggt gatgacgatg ctgcttccag
     6121 tcggacagat cactgataat attttgatga atgccctagt aggcggtgtt ttattaggtc
     6181 ttggcgtagg attatcttta aaaatgggct ttacaactgg tgggatggat atcatctctc
     6241 ttgtactttc acaaacaact ggaaaaacag tcggaaacta catgctgatt ctaaatggta
     6301 tcattgtagt catcgcaggc ttcttcttta actgggaaag tgccttgtat acgattattt
     6361 ccatttatgc aatgactcaa gtaattgatg ccatccacac gagtcatcaa aaagtaacag
     6421 caatgatcgt taccgtcaag ccagaagagg taacagaaga actagcaaaa cggatggtac
     6481 gtggaatgac attactgccg tctgtcggtg gctattcaaa aaaacaaggg aacatgatca
     6541 tgatggtcat cacccgctac gaaatgtatg atcttgatca gatcgttcat gaaatcgacg
     6601 acgatgcatt tatcaatatt ttaccaactc attcagtatt tggccggttt gcaaatgaaa
     6661 acgaacaaaa aatgtatcga acaactggcg tattgccaga aataaaaaat cgttcacaaa
     6721 aaaaatgagg aatcaggttt attttagaat aaatttaatc tattacatct aataagaaca
     6781 ttt
//
LOCUS       DACCXM010000129         6771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCXM010000129 DACCXM010000000
VERSION     DACCXM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.133"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(75..257)
                     /locus_tag="IVY61_002413"
                     /pseudo
     CDS             complement(75..257)
                     /locus_tag="IVY61_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            244..441
                     /locus_tag="IVY61_002414"
     CDS             244..441
                     /locus_tag="IVY61_002414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971486.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(484..1059)
                     /locus_tag="IVY61_002415"
     CDS             complement(484..1059)
                     /locus_tag="IVY61_002415"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7971487.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1214..1740)
                     /locus_tag="IVY61_002416"
                     /pseudo
     CDS             complement(1214..1740)
                     /locus_tag="IVY61_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            2197..3708
                     /gene="gadC"
                     /locus_tag="IVY61_002417"
     CDS             2197..3708
                     /gene="gadC"
                     /locus_tag="IVY61_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP7971488.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3721..5121
                     /locus_tag="IVY61_002418"
     CDS             3721..5121
                     /locus_tag="IVY61_002418"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP7971489.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5286..6721)
                     /locus_tag="IVY61_002419"
                     /pseudo
     CDS             complement(5286..6721)
                     /locus_tag="IVY61_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2087 a         1169 c         1222 g         2293 t
ORIGIN      
        1 aatcttttta ccactccaaa cagggagcgt caataatttt gtgtaaataa ctcgtccttc
       61 tgcaaaataa tgggttactc agtaaacatt gaagctaatg tatccgtcac ttgttggaag
      121 cctttatggc ttctatttaa gaatttttga ttgtatgtat caaagatgga tactaggaaa
      181 cgctctagtg attcttcatt ttgaaactgc tcttttctgc ggctgtattt cttgatttgt
      241 ttattgaaag attcgattgt agaaccagaa ggaatggaga acacttttga actttacaac
      301 aaaactactg ttgataaatt aattccaatt ttagttccaa ctgatttaaa tggtcatgat
      361 gttttacttc gttttaacgg atttttaggt gaggattata gatacctaaa aatttctgat
      421 cctcaacctt taacagaata ataaaaaacg tgtaatcaat tatttgatta cacgtttttt
      481 atttcaatct tcgttgtagt agtggttgcg acataccgaa aggtaatctt cattacctcc
      541 tactacaatc tgttcacctg aataaactgg aataccatca acaatacgag cattcattac
      601 agccttttta ttgcaaaaat ggcaaatagt tttaatttcc tcaattttat cagcaaatgt
      661 caataatgct tcggatccct tgaagagatt attttgaaaa tcattcttca atccatagca
      721 aattactggt acttcctcat tcattacgat aagtgataga tcaataatat tctctctact
      781 taaaaattga gactcatcga ccaaaataca atcaactttt ccgtactgat caataatctc
      841 cactaattct ccgtacacat ccatttcaga agtaattgca atagctggtt tcgaaacacc
      901 tatacgactc tcaacttttc caaatcctga tcgagtatct attgcgcttg tcattatgat
      961 aactctcttc ccttgttctt cataattatg agccactttc agtagctcaa tactctttcc
     1021 actattcatt gttccatatc tgtaaaataa ttgtgccatt aaatttattc ccttctttta
     1081 caattcaatt attccttaat tccatctgct tttatgcagc tgctcgttta ttccttgata
     1141 tgtctataac atctactatc cctaaaacca atttataagc cattagagat ggtattcctg
     1201 aacatctctc atattgtttt ttgagtggta tatcatctga aaaactgaag gaatcatgaa
     1261 aatagcgaca tacttatcat ttagagttct aactttaaat gcatttactt cttcagtcag
     1321 cactttagtc atgttggaca tggtttgggg cgtgtagtga tgcccataca ttttctcaat
     1381 caagtctgcg atttccgaca tggtaatacc ttttcggaag aggtgaatga cggtctcctc
     1441 taatgtgtca ttcgtccgtc tataagcagg aacagtctgt tgcttgaact cgccattgcg
     1501 gtcgcgcgga atctggagat gaagttcccc atactcggtc ttgaccgtac ggtcatagga
     1561 gccgttacga gaattacccg tgttaaaacc aatgcgatcg tacttttcgt aatcgaggaa
     1621 agccgttaac tccgttttga gaagcgtatt gacagctttt tccaaatgga cacggaataa
     1681 tccattcaaa tcgcctttgt tcgctagagt ctttaggatt tctgtagtaa aatcgttcat
     1741 atggaagtcc tcttttctgt gaatgtgttg tggtaactct attctacaga agggacttcc
     1801 ttttttctat ttacacaaaa tattttacac tctcaacaaa agtatcttcg attaatactt
     1861 gctcaagtta tgtgcagcta cgaaaaagta ggtaccgagg tactttcttt ttcgcagctt
     1921 tttttggact tatgagaagt acattcgctg gaagaatacg atataaaacg agctttttca
     1981 ctaaagatat taatagagtg aaagaaacaa gcttttgttg tcagtttttt cacttttcta
     2041 aaaatgaaaa taaaaaattc tggaagtgga gaagaatttt gcttgctata ctttgtcttg
     2101 tagaaaagag gtagacaaaa taatgcaagc tcaaataagc gattttccat aaccttggta
     2161 ggaatgacaa attaaatagg aaagggaagt aataatatga ataaaaaaaa gttatctcta
     2221 ttcggttttt ttgccctgac agcttccatg gttttgactg tttatgagta tccgaccttt
     2281 gcaacatcac aattacattt ggttttcttt cttcttttgg gaggtatttt atggtttcta
     2341 ccagttgctt tatgtgctgc agaaatggca acagttgaag gatggaaaga tggtgggatt
     2401 ttcagttggg ttagtcaaac tttaggagaa cgttttggtt ttgcagctat ttttttccaa
     2461 tggtttcaaa ttacagtagg ttttattaca atgatctact tcatactagg agcgctgtcg
     2521 tatgtaatta attttccaga gcttaataca gatccattta tcaagtttat tggtgtatta
     2581 gtcattttct gggtactgac tttttcacag ttgggcggga ccaatcgtac cgctaaaatt
     2641 gctaaagcag gatttgtcat tggaattgtt attccttcga tcattctctt tggtttagca
     2701 gcagcctact ttatcggcgg taatccgatt caaatggcta ttaatgcgaa agcttttatt
     2761 cctgactttt ctcagctttc aactttagta gtatttgtat cttttatatt ggcttatatg
     2821 ggcgtagaag cttcagcatc acatattaat gagctggaaa atccccaaag aaactaccca
     2881 ttggcaatgt ttttactagt gattttggcc atcgccttag atgcaatcgg cggtttctca
     2941 gtagcagcgg ttattccaca acatgacttg tctctcagtg ctggtgtaat ccaaacattt
     3001 gaaacgctta ttttacattt taacagtcat ttacattggt tggtaaaagt gattgctctc
     3061 atgattgcct gtggcgttat gggtgaagtt agttcttggg tagttggtcc atcacgaggg
     3121 atgtttgcag ctgctcagcg aggtctgtta ccagttttct tccggaaaac taataaacaa
     3181 gacgttccag ttccgttgat tatgatacaa ggaattatcg ttactatttg ggcagcagtc
     3241 ttaacttttg gtggaggagg caacaattta tcattcttag tagcaatctc cttaactgtt
     3301 gtcatttatc tcgtaggata tttactattt ttcctaggtt actttgtatt agtttttaag
     3361 aaaaaagact taaaacgaac ttacaatgta ccaggaaaaa ctgttggcaa aacgattatc
     3421 ggtggaattg gttttatttt atcagtgttc gcattgctta tctcttttgt accaccagct
     3481 tcaattgcag ctagtgagca ccgaacatac caaatcatac tacttattag ctttgtagtg
     3541 actgttcttt taccttttat catttatgaa gtacatgata aaagtgctca cacaactatt
     3601 gaggaaccta ctcacgtgaa agctgaagac gttaatcccg ctgtttatcc agctgctaga
     3661 ggagagcacc acgttacaaa aaatgatgaa catattttaa aacattagga ggaattcatt
     3721 atgttatacg gaaaagataa tcaagaagaa aaaaactatt tggaaccaat ttttggctct
     3781 gcaagtgagg atgttgactt gcctaaatat aagttaaaca aagaatccat tgaaccacga
     3841 attgcttatc aattagtaca agacgagatg ttggatgaag gaaatgcgcg attaaactta
     3901 gctacttttt gtcaaacgta tatggaacct gaagcagtga aattgatgac ccaaacgtta
     3961 gaaaaaaatg caattgataa atcagaatac ccacgaacaa cggaaattga aaaccgctgt
     4021 gtaaatatga ttgctgattt atggcatgct ccaaataatg aaaaattcat gggaacttca
     4081 acgatcggct cttcagaagc ctgcatgctg ggtggtatgg ccatgaaatt tgcttggcgt
     4141 aaacgtgctg aaaaattagg tcttgatatt caagcaaaaa aacctaacct ggtgatctct
     4201 tctggttacc aagtttgttg ggaaaaattc tgtgtatatt gggatgtgga actgagagaa
     4261 gtcccaatgg atgaaaaaca tatgtcaatt aatctagata ctgtcatgga ttatgtggat
     4321 gagtacacaa ttggtattgt aggtattatg ggtattactt acactggtcg ttatgatgat
     4381 atcaagggtc tgaatgattt agttgaagct cacaataaac aaactgacta taaagtatac
     4441 attcatgttg acgctgcatc gggtggcttt tatgcaccat ttactgaacc tgatctagtt
     4501 tgggattttc aattgaaaaa tgttatctca attaattctt caggtcacaa atatggtttg
     4561 gtatatccag gtgtgggttg ggtcttatgg cgtgaccaac aatacttacc agaagaatta
     4621 gtatttaaag ttagttactt aggtggagaa atgccaacta tggctatcaa cttctctcat
     4681 agtgcagcac aactaattgg gcaatactac aactttgtac gctatggctt tgatggttat
     4741 cgtgatattc accaaagaac tcatgatgtt gctgtttatt tagccaaaga gatcgaaaaa
     4801 actggtattt ttgaaatcat taatgatgga tcagaattgc cagttgtgtg ctataagctg
     4861 aaagaagatc ccaatcgcga atggacacta tatgatttat ctgatcgtct gttaatgaag
     4921 ggatggcaag tcccagccta cccattacct aaagacttgg atcaattaat tattcaacgc
     4981 ttagttgttc gagcagactt tggaatgaac atggctggtg attatgtaca agatatgaac
     5041 caagcaattg aagagttgaa taaagctcat attgtttatc ataaaaaaca ggatgtgaaa
     5101 acgtacggct ttactcacta aaattcttca gagaaatata aaagaagtta attaactaat
     5161 aaaaggagtt gtgattacca cacagctcct taaatctatt tgaataaata tatgatattc
     5221 atttgttaca ctctcaatct tagctgttgt tctattcatc attactctta ttgtaagtag
     5281 ttcagtcaaa tttgcttgtt caattgatcg ataacagtat gaattgtttg aaaagtaatc
     5341 ggatatataa ttgttttctc actacctaag ccttcttggt ataaagaact atcgcaaagt
     5401 ataatccgct gagtacaaaa gaaagtgtcg tctactattt ctctaagttg tttacaaatg
     5461 atattactaa tagaaaagtg ttctcccaaa atgtcttcta actgttgtcg atagtataaa
     5521 tacctaaagt ctgacggagc tactatgaac acttcaattg gagatttccg tttatgctca
     5581 tttaaaagac ttaaactaat ggttagttta cgaatagaat tctcattcca acgcagaaat
     5641 aaattgtttt gttttcgcca ttgttcaaga atttcatgaa cttctaagta caaagcgcgt
     5701 tgctcttcat ttaacaagta agtcttatca ggttgaaata tctgaatatc agcccaaagt
     5761 gagcggataa acgggagaag tgctttatca aaaagtatct gattttcttc agatatattc
     5821 agagataaga taagtttttg actgagctca atcaccaaag ggttatctgc tacatggttt
     5881 tgataaacaa cgttcaagtc tttttgtaac aattcaaagt tatcgcatag ataatttcta
     5941 gagttgaata gaaagtagat ataaaggcat tcatcttcgt catgacataa agagaaagaa
     6001 agttgctcta atcctttctg tattagctga aacaagggag tatgtcgggc aactcttttt
     6061 tcttcctgac taaaatatat tgggttagtc ttagatctct gcaaaccgat agcgatacct
     6121 aataaaataa attgtttttc aactgtaggg taacttcttg aaaaaaatgt ttttctacat
     6181 aatcaatcaa ttgttgaccg ctttgaataa ttgatttatt tagctggatc aactctgtcc
     6241 gatttgtctc atagattatc gttagtaaca aacttcgata atccttttca ggaatttcaa
     6301 aatggccatc agcatttaag tactgcattt gtttaaaaaa attaatcaac tcatttcgta
     6361 agttgtaaac tttgcttaac gaaatgaagg tagtatcagc tatattactc caattgtgtt
     6421 cccctgataa ataatgtcct agtacattga aaaagtcaga gcgctgatag atgagttgag
     6481 tgagtgccaa cctcgaaaac gaagagtcga atgcgactgt aaagctactt ctagatttct
     6541 ccactgaaat aatttgctga tggagtatgt cggtaatgca ttccaagtcg ttagtgacag
     6601 tgagtgggca aacgttcaat tgatctgcta tttcgcgaat ctgaatggtc cctttttcgt
     6661 gtaattgctc acaaatgtaa acttgacgaa aaatactttt ctccagatag gtttccaaca
     6721 tagtactcct cctagtcatt ttatacataa ctattataac tctaccaatc g
//
LOCUS       DACCXM010000130         6730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCXM010000130 DACCXM010000000
VERSION     DACCXM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.134"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            12..902
                     /locus_tag="IVY61_002420"
     CDS             12..902
                     /locus_tag="IVY61_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP7971490.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(975..1799)
                     /gene="nadE"
                     /locus_tag="IVY61_002421"
     CDS             complement(975..1799)
                     /gene="nadE"
                     /locus_tag="IVY61_002421"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP7971491.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1811..3283)
                     /locus_tag="IVY61_002422"
     CDS             complement(1811..3283)
                     /locus_tag="IVY61_002422"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP7971492.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3920..4282)
                     /locus_tag="IVY61_002423"
     CDS             complement(3920..4282)
                     /locus_tag="IVY61_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7971493.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4505..5257
                     /locus_tag="IVY61_002424"
     CDS             4505..5257
                     /locus_tag="IVY61_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7971494.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5397..6320)
                     /locus_tag="IVY61_002425"
     CDS             complement(5397..6320)
                     /locus_tag="IVY61_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7971495.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6335..6607)
                     /locus_tag="IVY61_002426"
     CDS             complement(6335..6607)
                     /locus_tag="IVY61_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971496.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2050 a         1347 c         1154 g         2179 t
ORIGIN      
        1 aggtgatata tatgcaacta caaataaacg aagaatcctt accggtatat gaagcattag
       61 ctagtaaaac aaggatcaag atcatccagc tactttccaa aaaaaagatg aatgtcaaag
      121 atttggcaaa agaacttgga gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag
      181 aggaagcaaa catcatcaaa acagaaaaag taggacaaca aaaaatttcg agccttcgtg
      241 tcgataaaat cgatatcagt tttccagaaa aaatattcaa tgcttttgat acgaaagaaa
      301 catcgatacc tattggtcac tatacaaatt atgcaattga accaacatgc ggtttagcta
      361 cgatccatga ttttatcggg aaagtggatg aaccacgcta ttttatggat cctcgaagga
      421 tggatgcccg cattctctgg tttaccagcg gatttgtcga gtatcaggca cctaattttt
      481 taaatacaga ggatacattg gagatgttgg aagtttctgt ggaaatttct tctgaatttc
      541 ctttttcaaa tgacaattgg ccttcagata tcactttcag cctaaatggt gtggagctag
      601 gaacatggac gagccctgga gattttgctg atatccgcgg taaatatacg cctgattggt
      661 atccagataa tcttaatcaa tacggcttgc tgaagacgat ccgaattacc aaacacttga
      721 cgaatatgaa tggagaaccc ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag
      781 acacttggca tttgcgaatc gaagtgaaag acgatgccaa acatgtgggg ggctgtactt
      841 tatttggtaa aggattcggt aattacgatc aggatatcaa attaaaagtt tactacagct
      901 gatatttttc ttttctattt tataaaacaa aaacagccaa tccctatgaa gggacggctg
      961 tttttgcttt attgttattt ccagaaatca tccaaaacgg aaattggcaa atgtcgttta
     1021 tgttgtgtct tgttccacca tgcttcgatt ttttcttgtg cttgagcggg aacttctttt
     1081 ccttctagat aatcatcaat ttcattatat gtgacaccta gtgcgacttc atctgcaatc
     1141 attggtttcc catcttcaag atctgcagtc ggaatttttg tgtataattt ttccggtgca
     1201 ttcaatgctt gaagcaattg tttcccttgt cgtttgtcca aacggaataa tggcaaaatg
     1261 tcggctccac catcaccaaa tttcgtgaag aatccagtaa tgttttctgc agcatgatct
     1321 gtccctaata cggctcctgc tctttctcca gctacggcat attgcgtaat catacgttga
     1381 cgtgctttga tatttccttt gttaaaatcg ctgattccca cacctgcttc gcttaatact
     1441 tgtacttgtg catcaacagc tggtttgata tttactcgca gactaacatc gggctggata
     1501 aagtcaagag cagcttttgc atcttcttca tctgcttgtt caccataagg aagacgaaca
     1561 gcgataaatt gatagttttc atcttttgtt tcttcacgca tttcttccat cgttagttgc
     1621 gccaaacggc ctgctaaagt agaatcttgg ccgccgctga tacctagtac aaaagtttta
     1681 agaaacggat attttttcaa atatgctttc ataaagtcaa tacttttacg tatttcttct
     1741 tttggatcaa ttgttggttt gacacctaat tcattgataa tttctgtctg caggctcatt
     1801 tattctcctc ctatgcttct gtatgaagtt ctgctacgct ttttttcatt cgttccatga
     1861 tagccatttt atggttccag cattcagtag acagatcgac tgggtatttt tgcggattca
     1921 agtctcgttt atactcttcc catagtgaat ctagattttc ttttgtatat tctttgattt
     1981 cgtttagtgc aggtaattcg taaactcttt ttccttcgat aaagatatct tgtaaaactg
     2041 ggcgagctgt aaagtctcga acagttttat tgataaacgt atgcactgga tggaacatga
     2101 agatagcttc ttcttctcga gggtcttcat cccataatgt cacgtagtct ccttcagatt
     2161 tcccatcaaa atttcgagtg atgcgccaca cttgtttttt ccctggagta gttacttttt
     2221 ctgcattgct tgataacttg atcgtatcca tcatttttcc ttcatcatct tcgatcgata
     2281 ctagcttgaa tactgcacca agggctggct gatcataggc tgtgatcaat tttgtcccaa
     2341 caccccatac atcaatttta gctttttgca tctttaaatt caaaatcgtt gcttcatcca
     2401 agtcattcga agcatagatt tttgcttccg taaatccggc tgcatctaat tgttcccgaa
     2461 cacgcttaga tatataggcc atatctccac tgtcgatgcg tacgccttgg aagttgattt
     2521 tgtcacctaa ttctttcgcc acgcgaatcg cacttggcac acctgatttc aacgtatcat
     2581 aagtgtcgac taagaacacg cagtctttat gggttttagc gtaggccata aatgcttcat
     2641 aatcatttcc ataagattgg actaacgaat gagcatgggt cccgctagct ggtatcccaa
     2701 atatcttccc agcacgtaca ttgcttgttg catctgctcc gccgatatat gccgctctag
     2761 ttccccagac tgctgcatcc agttcttgtg cccgtctagt tccaaattcc aatagtggat
     2821 cttctccgat gacagatttg atacgagctg cttttgtcgc gatcaacgtt tggaaattga
     2881 ccatattcaa cagtgctgtt tctactaatt ggcagtgcgc taaaggccct tccacttgga
     2941 ttaatggttc atttgcaaaa actagttctc cttcacgagc agaacgaact gttgctttaa
     3001 attcaaaatt ttgtaaatac tccaaaaaac cttcaggata gacttctaat ccacgcaagt
     3061 aagcaatatc ggaatcattg aacgtcagat tctctaaata attgactaac cgttccaatc
     3121 ctgcaaaaac cgcgtatccg tggttaaaag gcatttctcg aaagtaacat tcaaaaacag
     3181 catgtaaatc agctcgacct aattcccagt atgtctgcat catattgatt tgatatagat
     3241 ccgtatgcag cgtcaaactg tcgtctgggt aagtattatt catcttgtat tactcctctt
     3301 ggcaagtttt catttcctac ccattataac aaaaaaaatt aataggaaaa gaaatcttcc
     3361 attttttctg aaaaagcaca tgttttacgg atatttcttg ctaaaaatga actccatctg
     3421 ctaaaactat cctttcttat tggtatttaa cctaaacgag aaagaatact tattttattc
     3481 aatgtatccg agacacgaag aaattagcgt tttctatttt tcttacttga taaaataagt
     3541 agattttcct actacaatcg tagccgttaa gataccgatg attgaacaaa tataaccaaa
     3601 tgtctctaca acataatcaa tggcaaaagt ttttgaagca ataatacgtc tttccttttt
     3661 tgcttcttat ccccaaagaa acggatataa cttattatgt attgaatttt tttggaaagg
     3721 cgaattatga caagatatac cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt
     3781 ttaaaaagat acaaagtaag aaagcagctg tcaatagaat ggaaacaaaa atggcgagac
     3841 atcaatcagt ctcgccttgt tttttattgt atacattaga tatggagcag gttaccataa
     3901 agatgattat tagtcgttat taaatagaat ccttcgtatt tttttgttct atcactacaa
     3961 tatctttatt tggacgtttg aattgtattc cggcacgttc tagctcctgt ttcactccta
     4021 catggacttt gacatcttct cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt
     4081 cttgccttgt gatttttttc ttcgtattga tcatcacgac ttgcttttta tcatatgtgt
     4141 ctaccaatag cagttgtgtt cgatctaacg aaaataattt gtaataatta atagccgtat
     4201 tcccaaaaaa gaattccact actttaggat aaaatgtctt aatatttaaa ttactattca
     4261 caggtgtttc gattagcttc atctcattcc tcttttctat atagtcgatt tagatccaat
     4321 cttaagttat gttaccatct ttcctagcat taaggaatcg gttacaattg aacctttaaa
     4381 gacaaaagga tttacctttt atcacttaat agtataacat aatttcgtga gaaaaaaata
     4441 caattttttc ttgaccatct cgttacgtaa aggattatgc ttacatttgt caggaggaaa
     4501 aataatggag tacacaataa agaaaatgtc agagatatca ggagtcagtc cgagaacctt
     4561 gcgcttttat gatgagattg gactgctaaa accagctagg atcaattctt caggttatcg
     4621 gatttatggc aaaaaagaag tcgatcgtct gcaacacatc ttattttatc gaacaatggc
     4681 attcaagttg gatgatatcc aagaggtctt agacaatcca tcttttgacc accagaaagc
     4741 tttaatcaag catcagcaaa tgttgctgga aaaacgggca caaatcgata ccctcttgac
     4801 aactgtccaa cagacacttg atatgtacga aggaggaaga aaaatgagtg acaaagaaaa
     4861 atttgaaggg tttaaacaac aaaagttgaa agaaaatgaa gaatcctatg gacaagagat
     4921 tcgacaaaaa tacggaaaaa aaaaggttac agcatccaat gaaaaatgga tgaatatgga
     4981 taaagaaacg tatgaagaaa tgcaaactgt tgaaaagcag atgttatttg atctcactct
     5041 ttacttaaaa gaaccagaag atgaaagctt agcagaccgt atcttccaag ctcataagaa
     5101 gtggttctct tacagctggc ctgaatatca gccaaaagcg cataaaggac taggaatgat
     5161 gtacgaagcc gatgaacggt tcactgcata ttatgacgag cgaagtggtc atggtgcagc
     5221 aaaagcttta aatgaaatta tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa
     5281 agtacacgta aaatgaccta ataacaaaac agcagacacc caagttagat ttttatgcta
     5341 acttttggtg tctgctccat tctttcacaa gttttttcat cctactttaa atatattcat
     5401 cgttgaataa acgtataagg tattttcact ttatcctttt tttctgtaag aaacaattga
     5461 aatgccattt ctccgcaagc ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg
     5521 ctgatcagca aattatcgcg tccaatcacc actggtgtct tatcttgcag atagctttgc
     5581 aagatgcctg ctgcaaccat atccgaattt gtaaagacac catcaactcc aagctcttta
     5641 aaaaagaagc ctgcttgttt tcctttctct gcatctatac aatcccaaaa aacaagtgat
     5701 tcatcaaaag atggaaaaag ttgatgacat agctgcaaag tgatttttga gttataactt
     5761 agcctgccac ttcgccccaa cgtgatgccc aattgcttta taccttgctg cttcaaataa
     5821 gtaagacctt ctgtaattga ttgttctcga tcaataaaga cacagcttgc ctgttttccc
     5881 ttgatttctt cacaaaaaac tagaggtcca tatttttgat atgccaataa atcatctagt
     5941 gtattggcac gagacgtaat gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga
     6001 taccttcgct ctttttcttt atgatagccc gtcggtaaaa gtgtcacttt gtaatcataa
     6061 tcaaacgctg cttcaatgat cccactgatc aactgatcaa aatacggatg attggtataa
     6121 ggcagaatca ctccaatgct tttcgttttt ccataactta actgtctggc atttgcatta
     6181 gggatataat cgacttcttt catgaccgca agtatttctt tccttttttc ctcatctaca
     6241 tatggatgtc cattgattac acgtgaaata gtagaaactg aataacctgt gagctttgcg
     6301 atatctcgaa tgtttgccac tgctgcttcg ccacctatac aaaaagattt tcttgtaaat
     6361 agtctaccac agctggaccg cattcaaaaa actgatacat ttttccttca tgcgaaaaac
     6421 tgatccgctg cccgccccaa tatatatctt ttgtcaaatt ttcgatttca ttcattggta
     6481 tttcttttac tatattccta ttcaaccctt gaaaactgta caacgttaat acggatccct
     6541 tgaactcgac aaacgcataa aaataattgt tcgttgtgcc atctgttatc tctacataag
     6601 tattcattgc aagccctcct tttgatactt tgagtatagt gtatgaaacg cgtctcatag
     6661 caaggagttt gttttgccaa agaaaagttt tattcctctt tcattattac caaagtctct
     6721 tattttaaat
//
LOCUS       DACCXM010000131         6620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCXM010000131 DACCXM010000000
VERSION     DACCXM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.135"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            136..2811
                     /locus_tag="IVY61_002427"
     CDS             136..2811
                     /locus_tag="IVY61_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP7971497.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2993..3232
                     /locus_tag="IVY61_002428"
     CDS             2993..3232
                     /locus_tag="IVY61_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971498.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3248..4621
                     /locus_tag="IVY61_002429"
     CDS             3248..4621
                     /locus_tag="IVY61_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7971499.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4625..6259
                     /locus_tag="IVY61_002430"
     CDS             4625..6259
                     /locus_tag="IVY61_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7971500.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6422..>6620
                     /locus_tag="IVY61_002431"
     CDS             6422..>6620
                     /locus_tag="IVY61_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971501.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2320 a         1069 c         1330 g         1901 t
ORIGIN      
        1 aaatcacaac ttttttcttt tgtcaagatt ttgagtggga tatatttttg tagaactgaa
       61 gatcatgaaa ttatgagagg ttggaggtaa tgtagtcata caaataaaaa ttttttagaa
      121 agtgagtggt aattaatgaa aatgaatcac acttcaattc agaagcattt acaactgtta
      181 aatacactgg ggatttctta tgttaatttg agaacttttt ctacattaaa caagaaaacc
      241 gtttctcata ttttttctac tgcagattct caagcaatta ttaactattt agaaaaacag
      301 aaattgtctg aaggtacagt aaacataact tataatccta ttaaacaaga ggtgattaca
      361 caaaaaaagc attctgtgag agacaatgat attgaaaaaa ttcggtttgt atttattgat
      421 attgacccta aaaggaaaga gggaagtgca acagatagcg aaaagaaaaa ggcagaaaat
      481 gttatggaac aagtagaacg ttatttgaaa gaaaagagaa tagaatcatt tgtaaaagtt
      541 gatagtggga atggctacca tatttttctt cccataaatg aacaaccaaa taatcacgag
      601 acgattctta ctattcagaa ttttcttcaa ttacttcacc gaagatttgg agtagaagat
      661 gaagttgata ttgatacttc tgtttataat ccttcaagat tatgcaaatt agttggtaca
      721 ccagcggtca aaggaaagtc cacaaaagag cgtccgcatc gtttatctag tttcctttct
      781 attcccgata acatagatag aagtaattgt tttaaaattc ttgaacagat agttgaagaa
      841 aattccatga tgtcaacctc tagagtaaaa agccatccat cttttagaga agtgttacct
      901 tttattcaag cagactataa acaatggtta gctcactatc ctgaattaac ttataccata
      961 aaagagggcg atcataaagg agttacctta cttatatttg acgaatgtcc attaagaaaa
     1021 cacacgaata attctaatgg ttcttgtttg tctgttacgg ctgataataa gattcgattt
     1081 cattgtctgc atgcttcgga taaaaataaa gatattcaag actttgtaaa aaaatatccg
     1141 ttgccaaaac ctgtaaatcc ttctgtagaa tcattagaat ccaatcaaga atattatttt
     1201 gaccagttta aactgaatca agaaggagta tttttggtga aagaaggtca agaagaaaaa
     1261 gttgcttgtc cgatgtttat taagcaaata cgcaaaaata tcgaacaaaa agtcattaca
     1321 cttgttcttg cctattatgt cgatcattgt tgggatgaaa tagaaataga aggtggatgt
     1381 ttacaaacta ataattttaa acaattagca aaacatggct taatcattaa agctagaaga
     1441 gaagctgaag tcgttgactt tttacagatt caacgggaga atgctccaat cttttttgaa
     1501 catgaatctc ttggctggaa aaaagaaaat gaagcaatga tttttcagtt acaagattct
     1561 tttggacgtg aaattccttc tgtattaact aaaaactcat gctaccaatt aacaactaat
     1621 ggtacagaag cggaatttga cgcattggtt cataagtatg ttttaggaac gaacttagaa
     1681 cttgcttggt cgattggtat tgcttctatt gtgttaggct atttaaacgt tacgaaggct
     1741 aatacttttt tgagtttact aattaaccta cagggcaaga gcacaacggg taaaaccact
     1801 acgttacact ttattgcctc tttatttgga aatccaacta cggttcttag aaatatgaat
     1861 gctaccaata aagccattat aaagctagca tcaaacaatc agggaggtgt tccactaata
     1921 ctagatgagt taggagcagc aactcaaaat aactttagct cgttgatcta tcaattagct
     1981 tcaggagaag aaaggctaag attagattct aattgtcagc ttatgccaca agagactttt
     2041 tctgttttga cccttacctc ttctgaagag cgtttggaaa attatttcaa tgagaaatta
     2101 actggtttga aagttcgata tatcgaattt tctgatatca agtttacaaa ggatgcgaat
     2161 cacgcagaga aagttaaaag tctttcttct aatacttttg gttggggtat aaagttattt
     2221 cttttgaaac tctttgaaca agggattaca aaaattgaag caacttttgt cgaagctcaa
     2281 aaaaaactat gggctgtttt acctgaacat tctttgaagc aacgcattgg taataatcta
     2341 gctttgattt atagtggagc cctcctaatt caagatatac tgggctatcc tttacgatta
     2401 gagcatatac agacggtgct tattaattcg tacgaacagt taattgtaga tactagttta
     2461 ggaagtgagc aaacggtatt tgaccgagtg aaaacattgt tggtgatgaa tagccataaa
     2521 tttttaacaa acagtgacaa caaacatacg cccagtacga tttggggaaa agtatcaatt
     2581 caaaaaaatg gggatattaa ggtaaatatt tttcctatcc cgtttgagga cttgttaagg
     2641 cgagaatttc aagtaaagga tctaacctat ctttttaggc aattgcttga ttcgggtaat
     2701 atgtgtgcag aaaaagggag aagaacgaaa cgtattcaca tgaatcgtca agttcttcca
     2761 acatacgaga ttctattacc ttcttcactc aaatcgtatt ttcgattgta atttagaata
     2821 aaccaaagta tgaaagtgag gcaccatgtt atggaaatat ttcttattga tttagcaaaa
     2881 caactgatta cttatctgta tgatagcgag caaacccaaa cggaagtaaa tgaagcttga
     2941 tctatgcttc atttacttca ttcaatcaaa catgaaagaa aggaaaattc taatgactaa
     3001 aaataaaatg acactaaaag cagaggttct tctgtatatt caagaacatt tttcaaatca
     3061 agcctttttt acaaaaccaa tttacttgga tttcgaaata agaggggtat ctgctggatc
     3121 tatcgggggt actcttcaag cgttgaagaa cgaaggatat ttagaaaatc actttgtgca
     3181 acgttcgttc aatagaagag tcgtgaaaaa atggtattta gttcattcat agagagggga
     3241 atcaagcatg cgtgtggctt gttatgatcg aacgtctgtt tatgaagaag cgaaaattag
     3301 taatgacgct caatatgatg cagtgaaaga gtatgtttta gagaaatatg aacaaattcc
     3361 ccaacgattt tcagacaaag gagaatcagg ggaaaaagcg gagcgtccag cttttcgaca
     3421 gcttagagct tgggtttgtg cgtcagaaga agagggggtg atctatgttt ggcgttatga
     3481 tcgattggca cgtcaagcca aaatagcctt agaatttatt gagttgtgcc aacaacatca
     3541 ggttcaagtg tattcgattt ctgaacctct tcctgcttct ttctcacacg cgtcaacgaa
     3601 taaactattt gtgcaattat tgttcacttt tgcagaacaa caacgaaacg tcattattga
     3661 gaatgttcgt gccggacttt cctataaaaa gcaacaacgg caatatttgt cttctgcggt
     3721 accatatggg tatcgtttag tgaatgggaa aatccaacaa gaagtacacg aagcaaggat
     3781 tgttcgctat atttttcaac tttatttgac caaaaaatat ggctacaaaa agctttgtca
     3841 acgattgacg caacaaaagc ttttcttccg cgaacgtcct tttcagccct atcatattta
     3901 tagtatccta aaaaatccat tgtactatgg cgaaatcaaa ggaggatctt tggggaagta
     3961 tctaggaacc tttgaaccga ttctctcgaa agcaaccttt ttacaagccc aagagattcg
     4021 ccaatcaagg tgtacagcta aaaaagatac gtatctgtac ttattacgtc aaaaaattcg
     4081 gtgtcccttc tgtggtcgtc atctgagttc gaagtatcaa tggaatacaa agaagacgaa
     4141 gacgcttcat tattatcatt gtaccgaccg aagctgccaa ggaatttttc ttcgggcaga
     4201 aagggttgag tcagaagtat tggaaaagat acaggctttt tttcaatacg aaggcgtcta
     4261 tcaagccttg attgcccaat taacgcaaca attagccgaa aagcgaaaag atgaaaaacg
     4321 caaagtaatc caacaaaaga aacaagaagc acaatggatt aaggcatttg aagagggcaa
     4381 ttgttcactg gaagaactca agcaacaact gcaagctctt caaaagccga tgtctacttc
     4441 ttcgtttctt tcactccaac agtgtgaagc aaaagtgaaa gaatggcttc aactaaaaga
     4501 cctctctagt cgacaattat tatgtgacta tgtttgtcgg attgaggtaa caaaggataa
     4561 aacagtttgt gggatttact tccgacaact gacacaaaat attttagaaa ggaacgaatg
     4621 atctatggaa atgatacaaa ccaaacgagt cgcattgtat attcgcgtga gtacacaaga
     4681 acaagcttta gaaggacata gtattcaagc acaagaacaa aatggacgac agtttgccga
     4741 acggatgggc tatgaagtgg tagaagttta tgccgatgaa ggaatcagtg ggaaatccac
     4801 aaaaaatcgg ttcgcctatc aacgaatgat gaacgatgct cggaaccatc gttttgatct
     4861 actagtgatt tggaaattga cccgtctcgg acgcaatatg ttagatgtct tacaaacagt
     4921 cgaggaatta ttgcgatatg acgtaggatt acattcgatc tcggaacaat ttgacgtgac
     4981 cacttcgaca ggtaaattga tgttacaact ccttgggagt ttcggtgaat ttgaacgcaa
     5041 ccaaattgcg gagaatgttc aaatgtcaat gcaaagctta gtgaaagata aaaagcgtta
     5101 tgcaggaggc cgacgtttag gctatgtcag tggtattgac acgaatggtc aaaaacaatt
     5161 ggtcattgaa ccagaagagg cgaaaattgt tcgtctgatc tatgcaaaat atttaggcgg
     5221 agcagggtat cgagcgattg ccaatttttt gaatcgtcaa ggatatcaaa cagtcaaaaa
     5281 gaatgctttt tctaccacgg cagtcaaaga tatcttacgc aataaaattt atgcagggta
     5341 tttagaatat gcgcgctaca tcaattggga aaccaaacga cggaagggga agaatccgag
     5401 accgatttta gtgaaaggaa cccatgaacc gattatttcg gaagaagatt atcaaaaagt
     5461 acaagaacgc cttgctttag aaggaaaaca acccaaatgg aaccatacag gtgaaaatgt
     5521 attgacaggc ttgttacgtt gtccagaatg tggcgcaccg atggcagcga gtaatgtcac
     5581 aaatacccta aaggatggca caaagaaacg gattcgatat tattcttgtt cagtctttcg
     5641 gaataaagga gcaagtgtgt gtcatgccaa ttcgattcgt gccgaacaag cggaaacatt
     5701 tgtcgccgac cgattaaagg agatcgttca gctaccagaa gttcttcccc gattggtggc
     5761 agcgttaaat gaagaaatag tgcgacaaag ccaaccattg gaacaagaac tcgtggtgtt
     5821 actggaacga aaggaagaat tgaagactaa aattgaaaaa tgggaagctg ctttggaaga
     5881 tagtcctgaa ttgtttccga tgttaaaaga ccgcttagac gaattgactg aaaaacgacg
     5941 acagttgcac attcgagaaa atgaaatctt agggatcttt caacaaggag agccgattca
     6001 agtcaaagat gtccaacgga tattgactag tttggatcgt tttcttgccc agagtgagaa
     6061 gaagcaaatc aaagccttgt atcgtacctt tatagagaaa ataacctttg atcctaacca
     6121 taaggagatg ttggaaatta cgatgaaatt cacgcatgcc gtggtacaac aattgaatga
     6181 gcaatatcaa atagccgtat ccaaaacaaa ggatacggct atttttgtgt taaaaacacc
     6241 ctttacgtta acgatctagt ctaggttata tcaaacaacg ttagaggggc gctaacgctt
     6301 ctctagcgat ttgaaatcgt tagagaagta gttagggagg tccttatcat aaatagggat
     6361 atttattaat gaatatttga aatataattg tatataagaa ttaaaaagag tggggataga
     6421 tatgaaaaag atgggtaagt gtattttatg tgaaaaatac actgaactta ctaaagaaca
     6481 tgtaccacct aaaaaatcag ggaatactgg aggtaaaaag accagaactg gtaatttaga
     6541 tgatttcctt aagagcgatt ttacaaaagg tgattttccg aaggggatta aaaggaaacc
     6601 tcaaggaaat gtttattata
//
LOCUS       DACCXM010000132         6475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCXM010000132 DACCXM010000000
VERSION     DACCXM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.136"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(1154..1819)
                     /locus_tag="IVY61_002432"
     CDS             complement(1154..1819)
                     /locus_tag="IVY61_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7971502.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1945..3981
                     /locus_tag="IVY61_002433"
     CDS             1945..3981
                     /locus_tag="IVY61_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP7971503.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3985..4623
                     /locus_tag="IVY61_002434"
     CDS             3985..4623
                     /locus_tag="IVY61_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7971504.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4651..5079
                     /locus_tag="IVY61_002435"
     CDS             4651..5079
                     /locus_tag="IVY61_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971505.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5536..6447
                     /locus_tag="IVY61_002436"
     CDS             5536..6447
                     /locus_tag="IVY61_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7971506.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2233 a          977 c         1204 g         2061 t
ORIGIN      
        1 gattaaatgg taagtgtttc cttgtttgtt ttagtttaaa ctagtgtgta atttaaaaat
       61 ctatatggaa gggtctgtgg ggtaatactt aaataaaggc taggaacctg ataacatcaa
      121 ggttcctagc ctttttataa taaaatgaag cgaaaataat ttcttttaaa gagccgagat
      181 tatcagctat ataagagtga atttgatgca aaaaacagag ccgaaacagt cttggtgttc
      241 gtagctctgc ttatatgttt cgaatttaag acaaagagtt ggcaaaagat gttgataagg
      301 aagataagat aactgtgtta tataaactag gtaactaact gtaacaagct aattttaaga
      361 gattcttatt agaagacgtt taggaatata actaagaaaa tgattataca ggtaattgtc
      421 atttcaataa tgttttctaa ttttcgcgtc tttgttttaa gaaaaaggcc agaatcagtt
      481 ggaaaaaagt tcgtacgata gtgaagaggg gcaaaatttc ctactaatat tcccactaac
      541 aagagataaa acggccaggt tggtgtgatt cctctataaa aaaatgccca tgctaccgaa
      601 atcccataga ttaacaactg aacaattttt acaatgaaaa aattgttctg ccattactca
      661 aagtctcttc ctccttgtat cgtttatttg tttcatttta tcattatgat aggcgcttga
      721 acaatccatt ttgtatttta ttgagtactc ttggataaac taccagcgat tatcatattt
      781 atttatgtta ctgtaagcca tcatataaaa gtaaatgagc agatcataga aatataaaaa
      841 agaactaaaa ttgatctaaa ttatgtgcag cttgactacg taccaatcta tattacttac
      901 tagattggtc gttaatctat tcggatcgaa tgagcaaaat atttttgtga atgtccatcc
      961 ttgattatga ttccaaaaag aatcattggg aggctgtggc gtaaatatta tactataagc
     1021 cagaaatacc tatcattctg aactttttta tttaggaact ttcatctcaa ggctttttca
     1081 attaatttgt acccctgttt tttggataag atcatgtgca ttgatttttt ctatctctac
     1141 gttcatctcc tacttaatgc ctatctggct cctgagggct tctttttaag gcgtcgtcat
     1201 agtcttggct gaggatttct tggtaatctg agggaatggc ttcccaagtg agcaggcgaa
     1261 cgttcatgac gtgttttcct ttgtgtttga ttacagcatg gtttttctct ttttctacct
     1321 ctccagcgtg aaaagacagc catgtaccat ggccaaagat atcattacat acccaacgtt
     1381 cgatggaatt gtccacgtat cctacaggga gtgtggtata gccgatttgt ggtttcaaaa
     1441 aaggattgaa tcgatcgatc gtttcatttt tgtattgata attgataaga aaaagataga
     1501 ccaaacaaaa gacgagcaga aagaaagata gtgtcttttt gagtgagaag ggttgttttg
     1561 atgaataaga tttcttgtta acaagaggtt catctggcaa tgttggttct attgattcgg
     1621 tagctgattc aatagaagga aaatcaatcg gcagacctaa agtatgatgt aaaagtgaca
     1681 gcatttcaag gtctggcatt gtacgagaat tttcccaact agaaatcgtt ttatcggaaa
     1741 caccaatttt ttcgcctaat tctttttgcg taagcttaca atttctgcgg taagttcgta
     1801 ttttttctcc ggtgttcatc ttcttctcct ttcgattcta cagtatatag atttattcta
     1861 catgacctat acacttgata gatacgtttt tttgatgaaa aacggtatga ttttatcaaa
     1921 gaatcattta agtaagggag agtggtgata atcaaaaaaa agttcacagt tttactcaca
     1981 atcatagtga ccggtatcat gagtggtttt gccttggtta gtataaataa acaggtactc
     2041 tatcggacaa atgaattttt ccatcaaaca gatacttata ctccgattcg attgccacaa
     2101 cattttattg ggcaagaaga atatgagcag ctaatcatgg tatttgaaaa tgtctcatca
     2161 gaaactgagc tttcttatat gaaaaaaaca gtaaatgaat ccaatgaagt agatacatct
     2221 tggaacttca agatacagcc aaagcttgaa atgacattgt attatcaagg ggatgtcaaa
     2281 tcttctcacg agaaggtacc ctattctaat acctcctttc gcgtaacgtc cattgaagaa
     2341 tcgatcaaaa taaaagattt tgaaggtgaa tttttcttga tgacgagaga aaaagaagcc
     2401 tatgaacggt tcatttcact attcactgat cgattcaatc aagcatttca cactgattat
     2461 gccactaata tttttgaaga ctttgaaaat agctattcgt attcggttat ttcaaataca
     2521 gacgtatata attatcgagt gtatttaact attggattga ttcttttatt ccttttgtta
     2581 tcatcctttt tcttattgat gaataaagaa atcagtttat tgttgaataa tgacttttca
     2641 ttacatcaag tattgtggtg tttattgggg aaaaagaaaa tcattattac attcataatt
     2701 gcaggaatca ttggtggttt tatttttatg tattataagg agcaatttct ttatttgttc
     2761 cagatgtacg ttttcttgtt cagcggaata tatggtatct ctttgatttc tcttttatta
     2821 gttgaaaaaa tcgcttcgtt aagagaagcg ttcaactata tccaatacat tctttttaca
     2881 tttggattgt tgttttctct tcttttgacc gttgccaatg tcgaattagc aactatcgtt
     2941 ttttcttctt cacagttagc tgtttcccat gaaccaccaa aagaaatcgc tggtgaggac
     3001 tatcatgtat tttatccagt aaccatcggg aaaaaccacg tggattttat ttattccaat
     3061 cgttttgcat cagcagcaga tcaggaatta tatgaaactt tgaataaaaa cggaagtata
     3121 ttagtcaatg tatcagatta cttgaatgaa gaaaatgaac agtttggcag agggataaaa
     3181 ataaatctga attatcttaa gaaatttcct ttgattgatg tctctggaag gttgatccaa
     3241 ataaccgaaa aagaaacgca ccctgttata ttgatccctg aacgatatag acatacagat
     3301 ttaaaaaatg acctggcgca aataacagaa tattatcaag aaatcagtga aaaagaaccg
     3361 aaatttctat ttataaaaaa caatcaaccc atttatacat ttattccttc tatcccctgg
     3421 atcgaacatt atccggttgt tgaaatactg acattgaaaa acagtatgta tttggaaaga
     3481 aatattctca gcggagaaat ctatccaccg ttaaagatca agataggctc tttgtcgaaa
     3541 gaaaggttat ttgagctgat tgaagaaagt cagttacgtg ataacttgca gttatcattt
     3601 ccgtatactc agactgaaga gatagctgtc aaagtgttaa gcagatcatt tcattacttg
     3661 atacagatat ttttactcac tttcttttct ttcttcttgt tgagctatgt catgttatgc
     3721 atatattttg tctacaattg tcggaaaatc gctatctttc gttcaagtgg ttattcgctc
     3781 tttgaaacgt ataaagattt cttcatgatg aacctcatca aatggggaac gacaagtgta
     3841 atcttccttt ttcttataga aagagagcct aaatatcttt ttaatatttt tttctttagc
     3901 ttcattgatt ttattctttc agttgttttt attttgtcta cagaaaagaa aagtcagtta
     3961 ttgctgatga atggaggtta acaattggag ccgttgatag tgataaaagg tgcgaaaaaa
     4021 atcattcgaa acaaaacgat tcttcatttg gatcgtttca ctgcggatgc tggggatctt
     4081 gtgactgttg ttggagaaag tggagcaggt aaaagtacgt tattgaatat attaggattg
     4141 aatgatcagt tcagttcagg acaattctat ttgttcgatc aagagacgaa acaattaact
     4201 gggagagaga agcgcttgat aaaaagaaat accatatctt ttctttttca agattttggg
     4261 ttgattgaat atgaaacaat cgattttaat ttagatattg gattgaagtt ttcgaattta
     4321 aatgtttcag ataagcgaac agcaaagtat gaggcattga aaaaagtaaa tataaacaag
     4381 aagctaagta cgcctatcta cacattatct ggtggtgaga agcaaagaat cgcctgtgcg
     4441 agagtcttat tgaagaatag caaaatcatt ttagccgatg aaccgactgg gtcattggat
     4501 aaaagaaata aggataaagt agttgaaatc ttatcgcaac aagcttccca aggtacgtta
     4561 gttatcgtag tcacgcatga tccatatctg accaaaatag gaaacaagaa gatcatgctt
     4621 taaaaatcaa aaaggaaagg atgtcgtttt atgaaaaaag cagttattat ttgtgcacta
     4681 gggataggtt ttgttggagg aaacgctatg tcatctgaag tggaagcaaa ttcagttcac
     4741 ggtgaaatct attacaaatc tttaaagcca ggagagattc attttgatgc taaaggtaat
     4801 attattaagt caaatttacc agtagaccgg gaaatttcta gaacagcaat taatcatagc
     4861 tctggaagat gggtttatta tagtgataca acttgtacga gaaaaacagg tcattctaac
     4921 cactatagtc gtgtctataa tcgtcacggt gcaaaagcga aagttggaaa ctctattcga
     4981 tcagcaaatg caacaaaagg agtatgggcc tatgcaagtg cgacaggaaa tagaactgac
     5041 acattctcat gttggtacaa tccaaccggt tggtattaag tatccaaaag tttatataaa
     5101 atagaaaaaa catataatga aacagcgtta aaagttattt ttggtagttt taatatattc
     5161 atttttagtt gtataaaaat atatcataat aaaaacaaca aaaaaatgtc tggaaaatcg
     5221 aacataaaag tgtctgattt gaatatccca gcagcggaag aagaaaaaca aaatcgataa
     5281 catttgttct aagaattagt tacgttaaat acggaacgca agatagactt gaaaaacggt
     5341 aacaaaagaa gtagtatatt aaggtgtgat gtgctacttg acatttattc tgctgagcga
     5401 actttctgga ttgtgtagcc ttatatctat tcaggacgta tcgagcagat tagtaaaggt
     5461 gttcttgatg attttcatct tgaacacctt tgtttttagc aattcgcaaa aaacgaaaag
     5521 taggagaatt gataaatgaa aaaaagattt ttagcacttg ccatcgtact aggcactggc
     5581 ttattgtcag gctgtacgaa tgccggtgaa aaaacagccg ttagttataa aggcggtaca
     5641 atcagcgaac aagaagtaat ggatagcttg aaaaaaatgc aaggagcaga ttccgctgtg
     5701 caacagttga tcgtttatca agtatttgaa gataaatatg gagatgacgt atcaaccaaa
     5761 gaaatcgatt ctcaatatga tcaaacaaag aaacaattgg gtgattcatt tgattcacaa
     5821 ttgaaatctg caggttacac agaacaaaca ttcaaagaca gtatcaaaca aagtctagcc
     5881 tttcaagaag gtctaaagaa acatatcaaa ttgaccgatg aagatttaaa aacggcttgg
     5941 gaatctttcc atccagaagt ggaagctcaa attatccaag tagcaagtga agatgatgca
     6001 aaagatgtga agaaagctgc tgataaggga gacgatttct ctaaattagc caaagacaag
     6061 tcaactgata ctacgacaaa agaagatggc ggaaaagtga aatttgattc cactactaca
     6121 acggttccgg cagaagtcaa agaagcagcc tttaagttga aagatggtca agtttctgat
     6181 gtgatcactt ctaccaatgc ttcaacttat actacggaat attatgtggt taaaatggtg
     6241 aaaaaccaaa acaaaggcaa cgacatggat aaatacaaaa aagaattgaa agaaattgca
     6301 acagacacaa aattaagtga cagtaccttc cagaataaag taattggcga agtattgaaa
     6361 gacgctaatg taaaaatcaa agacaaagac tttgaaaatg ttttgagtac attcacttca
     6421 gattctagta cagcaagtag taaataagcg gtataaataa aaaatatgaa ctatt
//
LOCUS       DACCXM010000133         6417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCXM010000133 DACCXM010000000
VERSION     DACCXM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.137"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(166..354)
                     /locus_tag="IVY61_002437"
     CDS             complement(166..354)
                     /locus_tag="IVY61_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP7971507.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            624..986
                     /locus_tag="IVY61_002438"
     CDS             624..986
                     /locus_tag="IVY61_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7971508.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1038..2720
                     /locus_tag="IVY61_002439"
     CDS             1038..2720
                     /locus_tag="IVY61_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP7971509.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2837..4873
                     /gene="recG"
                     /locus_tag="IVY61_002440"
     CDS             2837..4873
                     /gene="recG"
                     /locus_tag="IVY61_002440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP7971510.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4922..5923
                     /gene="plsX"
                     /locus_tag="IVY61_002441"
     CDS             4922..5923
                     /gene="plsX"
                     /locus_tag="IVY61_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP7971511.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6045..6287
                     /gene="acpP"
                     /locus_tag="IVY61_002442"
     CDS             6045..6287
                     /gene="acpP"
                     /locus_tag="IVY61_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7971512.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2131 a         1126 c         1457 g         1703 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaataaaaaa ccgttcacta tctgaacggt ccaagtatca ttcaattaaa cgcgttcaac
      181 ttttcctgat ttcaaagcac gagttgacac ccaaactttt ttaggttttc cgtcgaccat
      241 aacgcgaact ttttgtaagt taggtttaac agtacgtttt gttgagttca tagcgtgtga
      301 acggttgttt ccactttttg tcttgcgacc tgtaaagtaa caaacttttg ccatggtaat
      361 ttcctccttt gccttgattt ggagcaatag aataattcag ctcataccag actaacatat
      421 catatagaat ggaaatttgc aagtacattt tcatagatac aattgacaat tgagaagaat
      481 aattgtaaat taaacaaggt tatacctgaa attttgcaag gtttttgagt actttgggta
      541 actgcctttt atttcaagtt gcgctatgat aaaatatgat agaagaaaaa tgggagtttt
      601 ttacccgtta aggaggctca ttcatggctg taaaaattca aacgtcagct ggaaccattg
      661 aaattaccaa tgatgtaatt gcaacagttg ttggtggcgc cgcgacagat gtgttcggtg
      721 ttgtaggaat ggccagcaaa aatcaaatta aagataatat tactgagatt ttgcgtaaag
      781 aaaattactc aagaggagta gttgtccgcc aagaagataa tggtattgct gtagatgttt
      841 atacgattat cagctatggt acgaaaatct ctgaagtttc ccgcaacgtc caagaaaaag
      901 tgaaatacaa tctagaggca ttattaggcg taactgccaa ttcagttaat gtctttgtac
      961 aaggtgttcg tgtactgcca gactaggcag gagactggct ggtaactcag cagtcgatct
     1021 gaaggaggat tatctaggtg agtgtaacag aaataagtgc aagccagttc caggaaatgg
     1081 tccaggcagg tgcgaatcgt ctgcaagtaa atgcagaata cgtcaattca ttaaatgtat
     1141 tccctgtgcc agatggcgat acgggaacaa atatgaatct atctatgact agtggagcta
     1201 aggcagtagt agattcaacc tcagaaaaag taggagaatt agcagcaatg ctttcaaaag
     1261 gattgctgat gggtgcgcgc ggaaattccg gcgttatttt gtctcaattg tttcgtggat
     1321 tttctaagca aattccagat gttgtctcat taaatgcttc cgatcttgct gcagccttca
     1381 ctcatggagt tgaaactgcg tataaagcag taatgaaacc agtagaagga acgattttga
     1441 cagtagctag agaagcagcg aaagctggag aaaagaaagc aaaaagtact gatgatgtcg
     1501 ttgaagtaat gacagctgtt gtaaaagggg caaagcgtgc tttagctaaa actccagact
     1561 tgcttccagt cctaaaagaa gtcggtgtcg tagacagtgg agggcaagga ctgctgtttg
     1621 tttatgaggg ctttttaagt gcattgaatg gagaattcca agcggatgaa acatatgaac
     1681 cttctccagc agaaatggat gatatggtca atgcagaaca tcatcgcagt gtccaaggtc
     1741 aaatggcgac agaagatatc cattttggtt actgtacaga aatcatggta aaaatcggag
     1801 aaggacctac agtcgatagt acattcgatt acgaagaatt ccgtaactat ttagatggta
     1861 taggagattc tttacttgtg gtcaatgatg acgagatcat caaagtccac gtgcatacag
     1921 aaaacccagg agaagtcttg aattatggac aaaaattcgg ttcattagtc aaaatcaaag
     1981 tcgacaacat gcgcttgcag catgagacga ttttggaaca cgatgaagaa gtgaacgagt
     2041 ttgcgaaaaa agaaacacca cgtatcccgt atggtatcgt agcgatcgct gctggtaaag
     2101 gcgtccaaga gctgtttgaa agtttaggcg ctaattatgt tatcagtggt gggcaaacaa
     2161 tgaatccaag tacggaagac attgttaaag cgattcaaga agtaaacgca gataaagtga
     2221 ttgtcttgcc aaacaataag aacatcttca tggcagctga tcaagcagca gaagtagcag
     2281 atatcccagt agcagttgtt ccttcaagaa cgatttccca aggattgaca gctatgcttt
     2341 cattcaatga tcaagcttct ttagaagaaa acaaagaaac gatgacagaa atgttgtcta
     2401 gcgttgtgag cggtcaagtc actcacgcga tcagagatac ctcaattgac ggtgtagaga
     2461 tcaaagaagg agactttttg ggcatgatcg atggaaaaat cctgatctct aacccagata
     2521 tcacagcaac agcgttaagt acactagaac aaatgatcaa tgaggacaca gagatcgtaa
     2581 cgatcttggc aggtgatgaa ggaacagttg aacaagcaaa tgaatttgct gagaaactaa
     2641 cagaaaaaca tcctgatttg gaaatcgaga tccaccaagg agatcagcct gtctatcctt
     2701 atctattttc tgctgaataa tttctgtgtc agaatatctt tctaacacag tattcattca
     2761 taaaaggcct tcggttagtg atcaggtatg atcgctgacc aagtcctttt tttgttaagg
     2821 gagggaggaa aaatttatgc gttctttaga tgatccaatc actatgctgg caggagtcgg
     2881 agaaaaaaga gcagctagtc ttgccagctt gggcatccaa acgattgaag acttactgac
     2941 ttattatcct ttccgctacg aagatattca ggaaagaaat ctaaacgaga tccaagatca
     3001 agaaaaggtt actttgaaag gaatcgtagt ctcgcctcct gtgatgaatc gatttggtta
     3061 taaaaaaagc cgattgcaat ttcgtatgat gcaagaacat gatgtgttca acgtttcttt
     3121 ttttaatcaa ccttatctaa aagaaaaagt cattttatcg gaggaaatcg ccgtatacgg
     3181 taaatgggat gcaaagcgta aagcattgaa cgggatgaag attttaggtt ctcaaacaat
     3241 ggatgacttt tcgccgatat atcacgtcaa caaatcaatt cgtcaaacga cattagttga
     3301 tctaatcaaa aaagggtttc aagaatacgg cacattgatt gaagaaaatc tgccaaacaa
     3361 ccttgtggag aaatatcgac tgcttgaccg agcttctgct gtaagaagta tgcattttcc
     3421 aaaagaccac gaagaaaacc atcaagccaa acgacgtgtc gtttttgaag agttcttttt
     3481 atttcaaatg cgtcttcaag gattgaaaaa agcagaaaaa gcagaaacga atggtttgga
     3541 gatcctttat gatgtccaaa aattgaaaga atttactcaa aagctacctt ttgaactgac
     3601 aaatgcacaa aaacgtgtaa cgaatgaaat ctgtcgggat cttcgcagtc cgcagcatat
     3661 gcagcgactt ttgcagggag atgtaggtag cggaaaaaca gtggttgccg cgattgcgtt
     3721 atacgctacc gttacagcag gtttccaagg tgcgttgatg gtgcctacag aaatcttggc
     3781 acaacaacat atggaaagct tgcaacagct gtttgatccc aatgaagtaa ccatcgcatt
     3841 gcttacgggg tcaacgaaag caaaagaacg tcgtgagctc cttgaaaaac tagagcaagg
     3901 ggaaattaac atcgtgatcg gcacacatgc attgatccaa gatggagtcg aatttcagaa
     3961 tttaggtcta gttattacgg atgagcagca ccgttttggc gtgaaccagc ggaaagtact
     4021 tcgtgaaaaa ggctggcgac cagatgtctt atttatgaca gcgactccga ttcctagaac
     4081 attagctatt acagcatatg gtgagatgga tgtatctatc attgatgaac ttccagctgg
     4141 acgtatcccg atcgaaacac gctgggtaag aacacctcaa ttggacagtg tactggattg
     4201 gacgaaaaaa gaactggcaa gaggacatca aatgtatgtc atctgtccgc tgattgaaga
     4261 atcagaagca ttagatgtaa aaaatgcagt ggaaatctat gaaaaacttc gtgaactatt
     4321 tgcaccaaaa tatgaagttg gcttgttaca tggtaaaatg aagaatcagg aaaaagaagt
     4381 aatcatggaa gccttcaaag acaaccagat gcagatatta gtttcgacta ctgtaattga
     4441 agtaggggta aacgtgccga acgcgaccgt catgctgatc atggatgcgg atcgatttgg
     4501 attggcacag ctgcatcaac ttcgtggacg tgttggacgc ggaagtgatg cttcctactg
     4561 tatcttagtt gctaatccta aaaatgaact aggtgtggaa cggatgaaaa tcatgacaga
     4621 gaccaccaat ggttttgtac taagcgaaaa agatctggaa ttacgtggtc ctggtgaagt
     4681 atttggcttc cgtcaatctg gtctgcctca atttgcgatt gctgacttgg tgacggatgg
     4741 aaatgttttg gaggtagcca gagaagaggc tgcagccctt tggcagataa aggattggca
     4801 gatgcttcct gagtatcagc ctttagcgca aagcctccaa atagaaaatc aggataatcg
     4861 ttatttcgat tagcagactg tgctatactt tatactgatg acggaaaaag gagtggaaac
     4921 aatgaaaatt gccgtagatg caatgggcgg agatcacgca cctcaagcaa ttgtagaagg
     4981 cgtggcttta gcgcagcaag attttccaga agtagaattc ttactttacg gaaaagaagc
     5041 agaaatcaag aaatacttaa caaatgaaaa aaacatcacg attatccata cagacgaaaa
     5101 aatcaatagt gatgatgagc cagtaaaagc aattcgacga aaaaagactg catcaatggt
     5161 ccttgctgca caagcagtga aaaacggtga agcagatgct gtattttcag cagggaatac
     5221 aggcgcgcta ctagcagcag ggttgttcat cgtaggacgt atcaaaaata tcgaacgccc
     5281 tggcttgatg tctaccttgc cagtagtagg gaaagaaggt gccggtttcg atatgctgga
     5341 tcttggcgct aacgcagaaa acaaaccaga acatttgttg caatacggta ttttaggttc
     5401 tttttatgca cgtaaagtcc gaggtatcgg acgtcctcgt gtcgcactct taaacaacgg
     5461 aacagaagca acaaaaggta gtgaagtgac gaaaaaagcc tatgaactcc ttcagaatga
     5521 aacaagcttg aattttattg gaaacgtaga agctcgtgag ctactcaatg gtgtggctga
     5581 tgtagtggtc acagatggat ttacaggcaa cgctgttttg aaatctattg aaggaacagc
     5641 aatgaatatg atgaatctat taaaatctgc tattctaaat gaagggatca aaggaaaaat
     5701 gggcgctatg cttttaaaag acggcttacg ttctttgaaa gcagaaatgg attattccaa
     5761 acatggcggt gccgttttgt ttggcttgaa agcaccagtc attaaaactc atggagcaac
     5821 aggccccgat gctgttcgct atacgatccg ccaaatccac acaatgcttg agacagatgt
     5881 agttggacaa ttagtcgaac aatttgaaaa aagcgaagaa tagtatttgg atatctgaag
     5941 caatccgttc cattgaaaaa aaggtggaca ttcctgaaaa atgccagtaa aattagccat
     6001 ggtgtaggta attgacaagt gtatgattct tggaggtgaa aaagttgact cgcgaagaag
     6061 tatttaacaa agtagcgaaa attatttcaa atcattttga gatagatacc gataaagtga
     6121 cggatgaatt gaacatcaaa gatgatttaa aagctgattc aattagtatt atggaattcg
     6181 ttttagagct tgaagatgaa ttcggtacgg aaatttcaga tgaagacgct gaacaaattg
     6241 aaacagtcgg tggcgcagta gattatatca gcagccatct taaataattt caaataaaag
     6301 caaagaagga taaaaagctg catacagctt tttatccttc tttttttaat ctttttgaca
     6361 cgtaaaagtc atttttctga taattctggc gataaacaga aattatgttg caaagta
//
LOCUS       DACCXM010000134         6372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCXM010000134 DACCXM010000000
VERSION     DACCXM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.138"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(99..1229)
                     /locus_tag="IVY61_002443"
     CDS             complement(99..1229)
                     /locus_tag="IVY61_002443"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP7971513.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVY61_002444"
     CDS             complement(1878..3080)
                     /locus_tag="IVY61_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7971514.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVY61_002445"
     CDS             complement(3140..3343)
                     /locus_tag="IVY61_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7971515.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVY61_002446"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVY61_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVY61_002447"
     CDS             4880..5692
                     /locus_tag="IVY61_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7971516.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1907 a         1304 c         1005 g         2156 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aa
//
LOCUS       DACCXM010000135         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCXM010000135 DACCXM010000000
VERSION     DACCXM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.139"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            725..811
                     /locus_tag="IVY61_002448"
     CDS             725..811
                     /locus_tag="IVY61_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7971517.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IVY61_002449"
     CDS             complement(932..1621)
                     /locus_tag="IVY61_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971518.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="IVY61_002450"
     CDS             complement(1866..2780)
                     /locus_tag="IVY61_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7971519.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IVY61_002451"
     CDS             complement(2762..3142)
                     /locus_tag="IVY61_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7971520.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IVY61_002452"
     CDS             complement(3592..3879)
                     /locus_tag="IVY61_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7971521.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IVY61_002453"
     CDS             complement(3899..4123)
                     /locus_tag="IVY61_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP7971522.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IVY61_002454"
     CDS             complement(4763..5296)
                     /locus_tag="IVY61_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7971523.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IVY61_002455"
     CDS             complement(5317..6159)
                     /locus_tag="IVY61_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7971524.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACCXM010000136         6093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCXM010000136 DACCXM010000000
VERSION     DACCXM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.140"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(50..1558)
                     /locus_tag="IVY61_002456"
     CDS             complement(50..1558)
                     /locus_tag="IVY61_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP7971525.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1717..1908
                     /locus_tag="IVY61_002457"
     CDS             1717..1908
                     /locus_tag="IVY61_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971526.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2111..3028
                     /locus_tag="IVY61_002458"
     CDS             2111..3028
                     /locus_tag="IVY61_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP7971527.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3021..4094
                     /locus_tag="IVY61_002459"
     CDS             3021..4094
                     /locus_tag="IVY61_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971528.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4242..4760
                     /locus_tag="IVY61_002460"
     CDS             4242..4760
                     /locus_tag="IVY61_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971529.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4741..5133
                     /locus_tag="IVY61_002461"
     CDS             4741..5133
                     /locus_tag="IVY61_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP7971530.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5209..>6093
                     /locus_tag="IVY61_002462"
     CDS             5209..>6093
                     /locus_tag="IVY61_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP7971531.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKM"
BASE COUNT         1993 a         1079 c         1230 g         1791 t
ORIGIN      
        1 atcgattaag ctaatagttt cgttttcaac atttataagt aaactttttc tattggtaat
       61 atagattttc ggaagggtag acaggtgcgc aggtaatatc gattcctagt tttttcaatg
      121 tttgttcatc atttttattg accatcacag tagagtgggc ttgtgctccc tcaagttcag
      181 ctagtttatc ataagcaact tgagcgattg gattcgttac tgcactgatt gataaagcga
      241 tcaagacttc attgatgctt agtgcatcca aagaggaatg aagtgtcttt gatttcaatc
      301 cttgtattgt ttcaagaact aatggtgaca ataaaggaat gtcatctgcg atatgggcta
      361 aatgttttac tgcatttaag ataactgccg ctgacgcatc catcaatgaa cttgtacgcc
      421 cggtaatgat gacttcatct ggtaattcga ttgcaatcac agcagaagga tcttttgtag
      481 gactttgcgc ttgaatctta tctgcatagt catgggcagg gactaccacg tttcggtctt
      541 caggacgtaa accgacttct tccataatca gtttgattcg attaactgct tcttcgtcga
      601 tcaatccttt tttgaagtcg cattctgttt ggaaataacg acggatgatt tcttgtttag
      661 aggcttcttg gacaacggta tcatccacga tcccaaatcc tacacgattc acacccatgt
      721 cagtaggaga ttgataaaca gattctttac cagtaatttt ttcaataata cgtttgatta
      781 caggaaatgt ctcaacatcc cgattatagt tcacagctac tttattgtag gcatcaaagt
      841 ggaaggaatc gatcatattg acatctttta gatcgactgt ggctgcttca taagcaatgt
      901 tcagcggatg tttcaacgga acattccata ctgggaaagt ttcgaacttg gagtaaccag
      961 ctgcatgtcc tttttggctt tcatgataca gttggttcaa gcaagtagcg agctttccac
     1021 ttcctgggcc aggagctgtc actacaacga ttggtttagt tgtttcaata tactcatttt
     1081 ttccaaaacc atcttctgag acgatttttt ctacgttgac tggatagtct tcgatttctg
     1141 agtgtttata gaccttgatg tttcgtcttt ctagtttatt gataaagact ttcgtggcag
     1201 gctgtccact atagcgagtg ataacgacac tgttgattaa taagccataa ccacgtaatt
     1261 catcgattaa ccgcaagata tccatatcat aagtaatccc ataatcacca cggatcttat
     1321 tacgttcaat atctccagca tagacgcaaa tcagtatttc agcttgatct ttcaattttt
     1381 gcagcaattt gattttcgca tcttcttcaa atcctggaag tacacgtttc gcatgcttgt
     1441 cgtcaactaa cttcccacca aattctaaat ataatttgtc atagtggttc actcgttcca
     1501 aaatatacgc tgactgctct tcaatatatt tttctgaatc aaaaccaatt tttttcacta
     1561 ggttccctcc ttaagtacta aagcattttt tcattttttt gaggattcgt caactggaag
     1621 agcgaaattc ctcaaaaaaa caaagaaaat ggtttaaacc aacttgtcta gatagttatt
     1681 cattggtatg atagtttttg ataaggaagg tgctacatga aaaaaagaca gaaaaagaaa
     1741 aacgcatata aacactatat ccgttcgatt tttaccggtt atgaaagaat gcttgaagat
     1801 cccgaattgg aacaattgac gtttacttat ctaaacgaag aaacgcagct aactcgtgat
     1861 gagcatcagc gcattcattt cacaacaaga gatctgccta gtaaataaaa aataagataa
     1921 aggtcatcgg attcgattct gtcctatcag atggaaagac tagttgatcg aaaaaaaggc
     1981 tgtaccaaat tttggtgcag cctgttttat ggagcattgc ccgctctttt ttataacaat
     2041 tttttacaga aaatggtact attgaagtat taataagtga aaaacagatg agtaagagga
     2101 ggcaagtagt atgaaagagc cagatacaaa tgcaaattat gagccagacg aactagagga
     2161 gacgaacgcg gatcaggata caggacaaga atcgtcttct ttttacgata tcaaagaaaa
     2221 agcaagcatg aatctttccc aattattaga agacttacag gcttttgaaa aagctgtccg
     2281 cgaagaagat atccctgaga tctaccgttt atacaatggc cgattgaatg aagaattaaa
     2341 aagaagctca aatgaaaatc acgagatcga tcaattgctg atgcgtaaga tccatgatcg
     2401 ttttttacag gaatttcctt ttatggaaca agtagaaaac atctcgccaa cgatgagcta
     2461 ttacaaaatc ggcacttatt accatgatcg gcccacggtc ggtattgatg caagtctgcc
     2521 agaaattttt gttttgccta agatcgatga agagtgggag aaatattccc aaccggagtc
     2581 aacggactat gacaaaaaaa tcaatgagct agacgcaaag gtgatcactg ctcagacaga
     2641 aattgaacgt ttagatgaac aaataaaaga aatcaatcga caaatgactg atttgaacga
     2701 caataaagga ttcttgaatc gtaaaaaaat cgaggaagaa attcaagaat tagagaagaa
     2761 gaagcaagta ctatctaatg aaaaattagg ctggcttcct tatattgaga cgcctgaaac
     2821 aatccagcag caaaaagaag cattgaagca agaagcaaga gcagaccagc ttcgtgcagc
     2881 catcgtagaa aaagaacaaa ggcaaatcag acgctatttc ggtagcaaag agggattcgg
     2941 tcaagcaatc catgaattct tgatgaatta tcttggcaat gagaatccta ataatcaatc
     3001 taatgaagga ggttccgagt atgaatgaac acgatgaagc agaatggaca aaaaatgaag
     3061 aacaaacaga agatcatgaa gaaacgaaca cttcattgga agaacaaaca agtaatcctc
     3121 tggatgacac agaagaaatc cctatgctag aggaaacaaa aatacatgaa aaagaaaaag
     3181 taactccgac gaatacgaat actgcagaaa acacccatgc ttacgaaata actgaaaatc
     3241 ccgggtattt agaaggaaaa gagcaagatc atcaagatat cgtcagtttg attgaaacag
     3301 cggaagcaga attagcggat ttacgtatga gaaaagattt gatcgaatca gattttccac
     3361 aattattaga atcatatgat cgttttatat tgaatgaaga accaatctcc acgatcggaa
     3421 agacaagcct gatcgaatac ttcacgtatg aattactgaa accgttgaat gatattggtc
     3481 tggaacggtc aaattcagct tatgacacat ggaaaacaag acatttttcg atcaattata
     3541 cgttgaatgg acaaaacgaa ttagtctttt cctttgtatt accgaccatc gatcaacgat
     3601 accaagtgga atcgatgagt ctcctaaacg tatcaccaga gacaatggaa atcaatgtcc
     3661 aagatgaccg ggtgctttct ttgatccgac actggtcagt ggatcgagta ttctctgcag
     3721 ggcagatcac gatattcaat cacaaattga atcaattatt agcccatgca agaacacttg
     3781 gttttaccgt taatcagaca ttactagaca atacgaagcc acttcgtttg aatcttcaga
     3841 gcgaatttga attgacgaat gaagtattgg atgatatttt tatcgtggca atgaaaaatc
     3901 catcctacga tatggaaaag atcaacgaaa ctacttatca agtacgatta gataaaggac
     3961 agtctttaac gataacaaca aacgaaaaag atcaaacagt cttgtcgatc tcatctggag
     4021 actacccacg atctgtcatt gatttcttta tcaattacga attcttggtc ccattaatgg
     4081 tgagaaagtc ctgattggat aagttggatt accagatgtc attcagataa ttctgttcca
     4141 aaggacttgc ggcagttatc cagaataatt gtcacaagtc ttttctgtat gtactaaatg
     4201 gacgtgaata acttgctgat gaaggaggga tttcattagg aatgtccaca aataaaatag
     4261 cacagcttag cttattgtct gctgcttgtg tagcaggtag gatcgctttt caatttattc
     4321 cgaatttcca gccaatgact gctatttttt tatttatagt actttactta agcttaaaag
     4381 atgcgttaat tgtcatgagt ctttctattg cgatcagcag tttttatttt ggtgttggac
     4441 catgggtcat cggccaatgg atcagctata ctgttgttct gagtttgttt tcaatcgtct
     4501 gcttgagaaa aacagtgcaa aggaatctat ggttaaaggg agtctgtttt tttctggcgg
     4561 gcatggttta cggaataggt atgaccgttt ttgatacgct gttatatcgt ttgcctcaac
     4621 cttggatcta ttaccttcaa ggggtgtcgt ttgatttgat gcacagtata ggcaatgttg
     4681 tgttctttct tgtttttctg ccagtcgtca aacgttttta caatcattca ggaggaaaaa
     4741 atgaaaaaaa taatttatag tttgcttgca atgagtgtca gtatactcat tttgacaagt
     4801 tgcagtacaa cctatacaca atcggcttcc aaccaaaagg aggaagtaaa aacatcgcaa
     4861 aaagcaacaa tcacgttaca agaaaatggt caaaactttt cggaaaaaga agtggtcttc
     4921 aaaaaaggcg atgatttact gagtattttg aaacgaaatt ttgaagtcaa agaagataac
     4981 ggcttcatca cttcgcttga agggcactca caggatgaga aaaatcaact ctattggatg
     5041 tttactgtgg acgggaaaag tgctactact ggtgccaaag agatcaaatt gaatgatgga
     5101 caatcggtca ttttcaattt gtctaaacta taagtttcat aaaaaactgt taaagggcga
     5161 atattgccat aattcagtga gttcagttga tgaaagaagg aaaataacat ggaaaatttt
     5221 aaaaaaagcg gaatgttttc cgtaattgct gctctagggg ctaatattct agttgctatc
     5281 agcaaatttg tcggctatgc aatcagtggc agtgccgcca tgctaaacga aagcatccac
     5341 agtatcgttg attgcagcaa tcagattttg ctgttattcg gtgataaaca agccaataaa
     5401 ggacagagcg agttgcatca atttggtgaa ggacgagcaa aatacttttt cagtacaatc
     5461 gtcgcaatga tgcttttctt cggcggtggg gcacttgggg tgatggaagc ttttgaaaag
     5521 ttgacgcatc catcccatga agtgggtaat ccgatcattg ttatagcaat actgttattt
     5581 ggtttggtaa tcgaaggaag ttcgttacgc gtggcaatga aagaaatcaa agaattgaat
     5641 actgaaaaac tttccacgat gcgctttttg cgcgaaagtc gacacagtga aatactgatt
     5701 atttttacgg aagacttgtg tgctgtaatc ggtttggtct tggctttagc aggaacaata
     5761 ttgacttatg caaccgggat tgctgcattt gatgcgatca gcggattgct gatcggtttc
     5821 ttgctgatgg gagccgccat tttcctagca aaagaatttt atagtctgat catcggtgaa
     5881 agcgtgacag caagcgattt atcaaaaatc aagttagctt ttgaacgtcc tgagatcagt
     5941 cggctgatcg atataaaaac agttcattta agtccaacgg agattttagt tgcagcaaaa
     6001 gcagatatcg ttggtaacaa agagaatgaa gcttactcgg tcatcaatga tattgaaaaa
     6061 aatatgcgtc agtctcttcc tgacaagaag atg
//
LOCUS       DACCXM010000137         6065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCXM010000137 DACCXM010000000
VERSION     DACCXM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.141"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(128..1945)
                     /locus_tag="IVY61_002463"
     CDS             complement(128..1945)
                     /locus_tag="IVY61_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7971532.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(1959..2723)
                     /locus_tag="IVY61_002464"
     CDS             complement(1959..2723)
                     /locus_tag="IVY61_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP7971533.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            complement(2751..3449)
                     /locus_tag="IVY61_002465"
     CDS             complement(2751..3449)
                     /locus_tag="IVY61_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP7971534.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3461..4240)
                     /locus_tag="IVY61_002466"
     CDS             complement(3461..4240)
                     /locus_tag="IVY61_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP7971535.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4256..5200)
                     /locus_tag="IVY61_002467"
     CDS             complement(4256..5200)
                     /locus_tag="IVY61_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7971536.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5246..6025)
                     /locus_tag="IVY61_002468"
     CDS             complement(5246..6025)
                     /locus_tag="IVY61_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971537.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1565 a         1276 c         1110 g         2114 t
ORIGIN      
        1 agttcagagc aatctgctcc aatgattttt ccttccattt cattattgtt taatgctgtt
       61 ttaaatgcat tcactagttc acaacgacgt ccacaactta atattaaaat attcaatttt
      121 taatctccta tctattcgaa gcatttgcaa aagtgacaat gtctttagcc aattgttcat
      181 cgtcttgcgg caggctcttg acaaaatcca tcactttttg tattgagtaa cctttgatgt
      241 ttcctacaaa gattttctca taaactgctt catcatttct ttccttatcc aacaatagtt
      301 cttcatatag tttttcccct ggacggatac ctgtttcaat gatttcgatt tcgtcttcgc
      361 tatacccgct caatcggatc atgtttttcg ctaggtctac aatcttcact ggttcactca
      421 tatctagaat aaaaatttcg ccaccttttg ctaatgcgcc agattgaata actaaacgac
      481 tagcttcagg aatcgtcata aagtatcgag tcatacgaaa gtctgttaca gtgattggac
      541 caccttgagc aatttgttct ctgaatactg gaatcacact accgcgagaa ccaaggacat
      601 tcccaaatct gacagctgag aatttcgtac agccttcttc attcaatcca gtcacaatca
      661 tttcagcgat tcgttttgtt gaacccatga catttggagg attgttagct ttatctgtcg
      721 ataccatgac gaagtttttc acttttgctg ctttagctgc ttctgctaca ttttttgtac
      781 cgaaaatatt gtttttgact gcttcttttg gattatattc catcagtggt acatgtttat
      841 gagcagctgc atggtagacg atatctggat gatattgttc catgatctca ttgattttct
      901 cgcgatcttg gatatctgcg atgatcggta cgatttctgt aggacaattt tgatgatgcg
      961 tgcgtaactc acggtcaatc aagtagatcg agttctcccc atgtcctaaa agcaatagct
     1021 ttgcaggatt gaattgaatg atttgccgac agatttctga accgatcgat cctcctgcgc
     1081 cggtgacaag aatgactttg tctgtgatct gatctttgat ggattcaata tccagcttta
     1141 cctcatctct tcccagtaaa tccactacgt caattgtttt taattttgat acgctaattt
     1201 tccctgaagc aagttcttct atagaaggca tcgtgttgac tttcacacga gcggattcga
     1261 ctaactcgaa gattcgtcgt aatttttttc ttgataagga agggatagcg atcgttacca
     1321 tttgaatgtc atatttttca atcaattccg gaatatcttt taatgcacct aatacttttt
     1381 ttcctgaaag atacgtattt cttttatttg gatcatcatc tacaaacccg acaacgtgga
     1441 tatcttgagc cgttttggat ccaagaaagc tgttgtataa aatacgcccg ccttcgccag
     1501 caccaacaat caatgtattt ttagcagaat ctgctgatac gtatctcata ttttttgttt
     1561 cgatgtagat acgccagatc aatcggctac cgatgatcaa taagagcgaa agcagataag
     1621 caaagataac taaccgtaaa ctgtatcgcc gattaatgaa cagcagtatg aggatacttg
     1681 aggcagcact tgcagatagt gaactaaaaa ttgcaatgat ctctcttaaa tttgtatagc
     1741 gattgatccg agtgaaaact ttgaataaac taccataaaa taaataaaaa ccaatcgaca
     1801 aaccgataga gattaaaata agatccattg gtatagatac aaacggcttc atgaatttag
     1861 ctgctgcaat atttgcagca attaataaca agctgtccac aaaaaccaat gtggcaattt
     1921 ttcttcttct tgttaatata aacacttttt aattccccct aaaataaacc aaacttcttc
     1981 ttttttacct cttcatagtc tggataactg atttcatccc cgttgatcag atcctttgca
     2041 acttgcttca tctgtactac tttttcctta ccaaagtcct tggcaatctg ctcatagcac
     2101 tcttttaagt aaaaggtgcg cttagagact ccgtgtgcat cagaagcagt catctgcacg
     2161 agattatgtt tgaccattgt tttagctgtt ttctgaatat ctttgccaaa taccccaaca
     2221 taacttggcg cagtaagttg ggccaaacac cccatatcta ggaacggaag caaatgattc
     2281 gggtcttcgc gaaaatgcgc gttgcgttct ggatggacga tcactggtgt ctttcctagt
     2341 tctaacaact caaaaaaaag ccgttctgca tataacggca cttccagtgt cggaaattcg
     2401 atcagaagat atgtatcatc taaatctgta aataaaattt catctcggtg gatgtcttcg
     2461 atgagcgttc cggtaatacg tacttcttgt ccttccaata acgtcaaagg caactgtctt
     2521 ttctctaatt cggcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg
     2581 tattttccat tattgtggtg tggtgtgcat aaaatatgcg tgatgccttg ctgaatcgct
     2641 ttttcagcca ttgcaatact tgcttctaag ttttctgccc cgtcatcgat tcctggtagg
     2701 atatggcaat gtagatcgat catttctctt gtctcctctc ttcttattcc ttaatttccg
     2761 tagtaataac cagcatcttt gctatgttct gctccgttgt atacaacacc taaaactcgt
     2821 gcttgtacca tctctagcag ttcttttgct ttattcaatg attctttacg tgatgtattt
     2881 tcacgtacga ccaataaagt gccatccgtt tttgaagaca tgatctgtgc atctgttacg
     2941 gctactactg gtggcatatc gaatatcacc acgtcaaata actgtcttgc ttcgtctagg
     3001 atctgatcca ttcttggcga acttaacaat tctgatgggt tgggtggctt aggtccacta
     3061 ggcaaaatcg acaagttctc gactggcgtt cgttggatta catctgctac acttcctgat
     3121 gaactaagtg ctgtacttaa tcccctggca ttgttcagct ggaatgtctt atagacgacg
     3181 ggtttacgta aatctgcatc gactagtaga actcgttgac cggatttagc aaaaacgact
     3241 gcaatgtttg ctgcagtggt ggattttcct tctccaggac ctgatgaagt cacgacaatc
     3301 gttttgattt gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat
     3361 tgttcggcga ttggtgaaga tgggtttgtt aatgtgatca aactgactgc atgagtttgt
     3421 gctgcttttt gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc
     3481 tgcgtgatgg ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gttccttttt
     3541 tacggatacc agcgttcaat tctttcgctg acatttctgg aactgttcct aagattggga
     3601 atcctaatgc atccgtcaca tatttttcat ctttcactgt ccgatcaagt aattcgaata
     3661 agaatgccag acctacgcct accatcattc ctaatactaa gccaatcact agattcaatt
     3721 tattatttgg cgaaacaggt gtcgtattgg caactgcacc tgaaataatc gagattttat
     3781 cgacattcat cacgtctttt gcgttctctt ggaaaacttg tgaagttgtg ttagcgatct
     3841 gttgagcagt cactgcattc gtgcttattg cctggatcga gaacatttgt gaattttgtg
     3901 actgattgac ggagatcgca tctttgattt gtccagcagt cattttcaca ttgtcttctg
     3961 tttctaaacg atctttgact tcatttagta ctaaatcact gacgatcata tctttatacg
     4021 tattgatcat ttgcaggttc gtattgacat cattgacatt tgctgtgtct gattgtggca
     4081 gtgtcacgat caattgtgct tgtgaactgt attttggtgt caagatgaaa aatgttacga
     4141 ttcctgagat ggccaagcca gcaaacattg agatgatgat tgttaccaag tgtttgcgta
     4201 atgtttcaaa aatttctttc aagctgattg tctcttccat tttttctcca ctttcctatt
     4261 tattctctag attcatttga tcttttaatt cttttttcac acgatcaagt tcttgatcgc
     4321 tgactttttg ataggagata ttatcttgca tgaagccttc tccttgcagc tgatcttgct
     4381 tgatcgtacc aaatgcctcc cgataatcta aagcgatttt tttcatttca tcaaagctca
     4441 tgtctgtttt catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt
     4501 ttaaactcaa tacttttttt gctacacctt ctacgatctt tctttgacgc tcttgtctac
     4561 catagtcacc atttggatct tcataacgca tacgagaata agataatgcc tgatccccgt
     4621 tcaatgtaat tttaccgatt ggaaagtcat agccatcttg tgagaaagtg aggtcattat
     4681 tgacttcgat accaccaact gcatcaacga gttcctttag ccctttcata ttgatagaga
     4741 catagtgatc gattgggatg tctacatatt tttgtagggt atccatcgcc atggcagctc
     4801 cgccgaatgc gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat
     4861 acgtatctcg aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actgtggcaa
     4921 ccatcatcgt atctgatctt ccttgatcga cccgacctaa atctccagta tcaatgccca
     4981 tcaatagaac agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt
     5041 gggttcgttc cactgattcg taagttttct caatagaatg tgagagatcc agatataatt
     5101 ttgctcctac tcctacaatt gctaatacta atacgataag aactgctaaa ataatcagaa
     5161 atattttttt ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac
     5221 cttgcttgaa tcaacgtttt aactgtcaat aacttatatt gctatagccc atttgctgag
     5281 caacagtttg tgcttttgtt gcgtatgctg cgcttatttt ttcattccgg atctgctcta
     5341 tttgactgag cgccgtcaaa tattgatcgt aagtagcggt ggcagtcact tgaccatcct
     5401 tcaacatcgt atcgaataac tgatctagtt gcgtgacttg cgcgatctga gtatcagcga
     5461 atcctagata ttgcataaca accgttttcc aaggactatc tttaaaaaag ctcatatttt
     5521 cacgtacacg agcgacatct gcacttgtca ccttttcttt gatgacgaca tcgtctacag
     5581 cttgctgcca gtttgataca tttttagtaa ataaatcgtt gacagctgtc tggatcttca
     5641 gttttgattc tgcactatct aactgtgtca cgattgcttt cttctcatct tggattgcaa
     5701 cagcttcttt aggcaattct gattctttga tgtcgtaatc atctgctgaa agtttgacag
     5761 aggatagctt atttgacaaa cttgtgattg tttcttctgt tatttcaggg tttagaaaat
     5821 ttttttcttt tgtactatac agcttgtcta cttcttttga taaagaagtc aaggtgtctt
     5881 tttgattggt gatggcagat tctgcataat tgatccgttg ctgatgttgc cgctgattat
     5941 tgatatggta accaaacaaa cagatcgcaa tagcacctgc acctaccgcc acagcagtca
     6001 atgacttgac gaatggcttt tccatagatt cactcctaag tattgatgat agataaatta
     6061 agata
//
LOCUS       DACCXM010000138         5852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCXM010000138 DACCXM010000000
VERSION     DACCXM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.143"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(451..954)
                     /locus_tag="IVY61_002469"
     CDS             complement(451..954)
                     /locus_tag="IVY61_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7971538.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1313..2338
                     /locus_tag="IVY61_002470"
     CDS             1313..2338
                     /locus_tag="IVY61_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7971539.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2661..3539
                     /locus_tag="IVY61_002471"
                     /pseudo
     CDS             2661..3539
                     /locus_tag="IVY61_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4015..4131
                     /locus_tag="IVY61_002472"
     CDS             4015..4131
                     /locus_tag="IVY61_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7971540.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5059..5397
                     /locus_tag="IVY61_002473"
     CDS             5059..5397
                     /locus_tag="IVY61_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971541.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5491..5721
                     /locus_tag="IVY61_002474"
     CDS             5491..5721
                     /locus_tag="IVY61_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971542.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1985 a          876 c         1075 g         1916 t
ORIGIN      
        1 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
       61 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
      121 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
      181 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
      241 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
      301 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
      361 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
      421 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
      481 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
      541 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
      601 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
      661 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
      721 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
      781 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
      841 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
      901 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
      961 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     1021 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     1081 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     1141 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     1201 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     1261 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     1321 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     1381 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     1441 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     1501 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     1561 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
     1621 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
     1681 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
     1741 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
     1801 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
     1861 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
     1921 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
     1981 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
     2041 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
     2101 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
     2161 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
     2221 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
     2281 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
     2341 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
     2401 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
     2461 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
     2521 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
     2581 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
     2641 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
     2701 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
     2761 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
     2821 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
     2881 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
     2941 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
     3001 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
     3061 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
     3121 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
     3181 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
     3241 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
     3301 tactatcacc caatcttact aaatagttga aaaaagagtc aaaaatctgt ctttttgcag
     3361 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
     3421 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
     3481 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
     3541 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
     3601 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
     3661 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
     3721 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
     3781 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
     3841 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
     3901 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
     3961 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
     4021 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
     4081 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
     4141 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
     4201 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
     4261 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
     4321 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
     4381 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
     4441 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
     4501 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
     4561 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
     4621 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
     4681 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
     4741 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
     4801 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
     4861 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
     4921 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
     4981 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
     5041 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
     5101 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
     5161 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
     5221 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
     5281 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
     5341 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
     5401 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
     5461 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
     5521 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
     5581 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
     5641 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
     5701 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
     5761 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
     5821 tccagagtat tttattttaa cttttgtata tt
//
LOCUS       DACCXM010000139         5838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCXM010000139 DACCXM010000000
VERSION     DACCXM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.144"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            46..837
                     /locus_tag="IVY61_002475"
     CDS             46..837
                     /locus_tag="IVY61_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7971543.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            803..1756
                     /locus_tag="IVY61_002476"
     CDS             803..1756
                     /locus_tag="IVY61_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP7971544.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2154..3128)
                     /locus_tag="IVY61_002477"
     CDS             complement(2154..3128)
                     /locus_tag="IVY61_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7971545.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3243..3677
                     /locus_tag="IVY61_002478"
     CDS             3243..3677
                     /locus_tag="IVY61_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7971546.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3802..4788)
                     /locus_tag="IVY61_002479"
     CDS             complement(3802..4788)
                     /locus_tag="IVY61_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP7971547.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5216..5638
                     /gene="arsC"
                     /locus_tag="IVY61_002480"
     CDS             5216..5638
                     /gene="arsC"
                     /locus_tag="IVY61_002480"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP7971548.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1878 a         1115 c         1038 g         1807 t
ORIGIN      
        1 caaagtcgaa caaaaattga cttttatgtt aaaggagtaa ttcatatgct gaaaaaagaa
       61 agacaagaac aaatcattac attattagag aaagattttt atttaactaa ttcagaaatc
      121 gctaacaaat taggcgtatc agaaatgacc atacgccgag atgtcactga attagcaaaa
      181 aaaaataaag taaaaaagct atatggtggc gttgaacgaa aggaacctta taatcgagaa
      241 ctatcgactc aagaaaagat tgatacgaat gtagccgaaa aaaaatatat tggtcaagtc
      301 atgaacagta tgattcccga taattctact atttatgtcg gtgctggaac aacgatccta
      361 tatgcacttc ctttcattca aaagaaaaat ctatttgtaa ttacaaacag tttgattgct
      421 ttcaactatt taaagaatca tacaagttat cggattttac ttactggtgg cgaattttct
      481 cctattacag aagagttcat tggggaagtt gcagaaagat cttttgaaag tttgaatgtc
      541 gatatctctt tcgctgcaac aaacggcata tttgataaca atgtgacaac cgctcaattt
      601 attgagggaa atattcagaa cgcagcgctc aatgcagcaa aaatcaaatg cattgttgca
      661 gattattcaa aattaaataa atcagacgtc tatactttta gaaaattaac cgattttgat
      721 tatctaataa ccgataatca tttatcagaa caggatttca atcattattc aaaatactta
      781 acaatattaa aggagcccaa aaatgatact gacaatcacg atgaacccat ccattgattc
      841 catttatttt acaaaacact ttgaattagg tacgatgaca cgctttgatt ctccggtacg
      901 ctatgtaggc ggcaagggaa tcaattgcgg acgagcttct gctctactag gttcatcagt
      961 tattttaaca gggtttttgg gcggttattt tggcagtatg attcaagata tgctgaaaaa
     1021 tgaaaatttg tttgatttgc attttttacc tattgcagaa gaatcaagag gtgccgtcac
     1081 gatcatgcat gatgatgaaa cgcagacaga gctagtggaa gctggtcctg aaatagcaga
     1141 tactactatc aaaaaattaa tgaatgatat caaactaatc agtaaaaata attacctttc
     1201 agtcattaca ataaatggtt ctgttaacaa tccagatccc tttttgtatg ctaaattatt
     1261 acaatatatc aaagaacaaa tagatccttc cacctatata ttaatggata tatctggaca
     1321 acatctccgt gaagtcttag caagtaagta taaaccgatg ttcatcaaac ctaatatcca
     1381 tgagttttca gatatcatcc atcaaaaaat tactcaaaaa agcgacataa aacaccatct
     1441 aaaaacaaat ccactattta aagatatcga tgttatcatg atttcttgcg gctcgtatgg
     1501 tgcagtagtc aaagtaaaag aagaattttg gaatgttgag atccctaaga ttcctatcat
     1561 taatacaaca ggctctgggg atgccactgt tggaggtttt gctcattcat tagaacagaa
     1621 ttatccttta aaagaatgtg taaggtacgc aatggcttgc ggcatgtcga atgcacagca
     1681 atcagaagtc gggaaagtta ttccagctga tgtaaaaaat ttagcaaaaa gcattaaaat
     1741 cacagaaatg aaataaatag tacaaagtaa ataattgttt atatgaaatg agacacaaag
     1801 agactgtgac aaaaaacgtg tgtaccgacc cccaaaagtt agattttttg gtctaacttt
     1861 tgggagtcga tacattgtca cagtcctttt ttcgaataaa cggtgttctc ctgccaactc
     1921 ctcggtcaca agtcacaata ttcaacaaac atgagaagct attttttgaa attgctcctt
     1981 gtgactcaga gttaaacggc aggctcacga cctcttggta taaacggtgt tcaaaagctt
     2041 gcatctacgc taatgagtcc aaccaagtca aaaatgagga gcttttttgg ctgtccttat
     2101 tacttgatgc agggcagttt tttcataatc tcttttagat taaatccttt tggttacaca
     2161 aggttctctt caaagaatgt tgccattttt tcaaatggaa tgatgtctgt ctggtcgtat
     2221 agatctgtat gggatgctcc tggaatgatc atgagttcct tgttgtcacc ttttagttct
     2281 ttgaaagcgt cctccccaaa gtaacgtgaa tgagcttttt ccccgtgaat gactaagact
     2341 gcatgacgga tttctggaat ataatgcaat agttttgcat tcataagtga caaccctgat
     2401 gtcctattcc aaccatcatt ggagtttaaa gaacggacat gatatccgcg gtctgtttta
     2461 taatacgcat gatagtcttt gacaaattga ggagcatctt caggcaacgg atcaaccaca
     2521 cctccagcac gtgcgtagtc cccttgtttg aaatcaatga tgcgttgtgc atttaattct
     2581 tcacgcattt catatcgggc attttcatcc ataacatcga agtagccatt tgctgttact
     2641 cgagtcatgt catacattgt ggaaacaact gttgctttga cccgcgtatc gatagctgct
     2701 gcattcaatg ccatacctcc ccatccacaa ataccaatga ttccgattct ctcagggttt
     2761 acattttttt gtacagataa aaagtctact gccgcttgga aatcttccgt attgatatca
     2821 ggcgaagcaa catatcgagg tactcctccg ctttctcctg taaacgatgg atcaaacgca
     2881 atcgttaaaa agcctctttc agccatcgtc tgcgcatata gaccagacga ttgttccttc
     2941 actgcaccaa atggcccgct cacagcgatt gccggcaatt tcttttcact tacttctttt
     3001 ggttcatata aatcagcgac taatgtgata ccaaaacgat tcgggaagct cacctttcga
     3061 tgattgactt gattgctttt tggaaatact ttgtcccatt cttgtgtgat gtttaattct
     3121 ttattcatat tctagactct cctttgatca aaattgacat ttgtttttga atgtattatt
     3181 atgataaaac ttaaacttga ctttaggtca aggtaaaaat tttattttag gaggattttt
     3241 ttatgtattc cattggacaa gtgtctgaaa tgtttggatt gcctgtttct actttacgtt
     3301 attacgacaa agagggatta tttcctgata tccagcgtgt ttctggtatt agaaaatttt
     3361 cagagaatga gttagaggct ttaagagtca ttgactgttt gaaaaaatct ggtttagaaa
     3421 taaaagacat taagcaattc atggaatgga gcaaagaagg cagtaagaca tttgaggttc
     3481 gtaaagaact atttgaaaaa caaaaagaag tagtagaaaa agaaatagca aaattagaaa
     3541 gagtactcgc tatgctaaac tacaaaagct ggtactatga agaagcaata aaagccggca
     3601 atgaagaagc cgtattgaca atgattcctg atgatttacc ccaacatgta aaagaagctt
     3661 atgtacattc tcattgaaca aacggataac tcactctcct tctctatgaa cttcatgaaa
     3721 acatgaataa acacctctaa ctgattgtta gaagtgttta ttttttcact caagtatcct
     3781 gcacttacaa aaatatcctt tttaccgcca tcctaattct ggagcgatat cttttacgat
     3841 tgtttcaagc aagtgcatgt tatattctac ccccaaagta ttaggaatcg tgacaagtac
     3901 tgtgtctgct tctttgattg cttcatcttc agctagctcc ttgattaatt gatcaggttc
     3961 accagcaaaa gtttttccaa aaatcgttgg gtttcggtga taagcaagat agccgacagc
     4021 atcttgttgc ggactaccgt cttgtccaaa taagcgtcga tccaaatcag tggtgattgg
     4081 ctgaatcgag cgagtaacta atgttcttgg ttcaaaatca tgacctgctt gcttccacgc
     4141 ttctttatac gcacgtaatt ggtttgcttg ttggatatgg aatggctcgt gggattcgta
     4201 attgactaat gtagatgatt gcagattcat tccatgttgt gctgcccaga ttgccgtttg
     4261 ctgcgaacca gctccccacc agattctttg tcgtaatcct tctgaataag gttctatccg
     4321 taattttcct gatccttgtg ggaacattgg ttgaggattt ggttcttcaa atggttttcc
     4381 ttcaagtaat tccaagaatt caagggtgcg ttcccgtgtt acctctttga tttcttcttc
     4441 actgtaatca taaccaaagt atttccagcc atctagaact tgttcaggtg aaccacgccc
     4501 cactcctagt tgcactcttc cttgcgtgat gatatctgtc aatccggcat tttctgccat
     4561 ataataaggg ttttcatatc gcatatcaat aagtcctgta ccgatttcaa tcttacttgt
     4621 ctttgcgccg attgccgcta gcaaaggaaa cggcgaccca atctgagggg caaaatgatg
     4681 tacgcggaaa aacgctccgt ccactccgat tttttctgct tccaccgcta aatcaatcga
     4741 ttgcaaataa gcatcttgag ctgatttcac aagcgatcct tgctccatcc aatgtccaaa
     4801 gcttaaaaag ccgatttttt tcaaggtaat gacctccttt aaacctacaa aaatggaagg
     4861 ttttttattt ttaatatgaa ctgattctac tacttttttt cttaaaagac gaataattgt
     4921 tctcgcctca caatcaagaa atgtatcggc ttactacacc cctactttct tcatcatctg
     4981 ccgtgtctta atctctttta acagtctgtt acatcgttga tttttgactc aaaatgattg
     5041 tttctaacat gtatttatgg taaaaaagac aagattagtt ctttgtacac atcgatttat
     5101 acgctgaaca aaaagaaata tataaactaa gaaacccatt gttttttcgc tccatcacat
     5161 agagtttact ttagataaac agtgacattt actaatccat aacggaggaa atcaaatgaa
     5221 aaaaatctat ttcttatgta cgggcaattc atgccgcagt caaatagcag aaggctacgc
     5281 gcgtaaatac ttgtctcctt caatgtttga gataagaagt gcggggatcg agacacatgg
     5341 cttgaatcca agagctataa aaatcatggc agaagacggc atcgatattt ccaaacagac
     5401 gtctgatttg attgatagag attactttac tcaggcagac ttgatcatta cactttgtgg
     5461 tgatgccaaa gacaagtgcc ctgccgttcc aaacaatcac gaacaccttc actgggacct
     5521 aaaagatcct gcacaagctg tcgggagcga agaagaaata ttaacggaat tcagaaaagt
     5581 cagagaagga atcaaaagaa aagttttaga cttaaatcaa cgtacatcga tcgtataaag
     5641 aagtattcaa aatgattttt caagcactaa gaaacaatgt taaaaaaata gctttccata
     5701 ttttacaaaa ttgtgcctta ttgccgacaa attgtctctt gaacaccgtt taccagtagt
     5761 caagacatcg taagcaacta taaaaaatta taagaaactc aaaataaaag gatgtgccac
     5821 aactttggcc acatcctc
//
LOCUS       DACCXM010000140         5779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCXM010000140 DACCXM010000000
VERSION     DACCXM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.145"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..813
                     /locus_tag="IVY61_002481"
     CDS             <1..813
                     /locus_tag="IVY61_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP7971549.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IVY61_002482"
     CDS             807..1229
                     /locus_tag="IVY61_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971550.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="IVY61_002483"
     CDS             2363..3331
                     /locus_tag="IVY61_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7971551.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="IVY61_002484"
     CDS             3341..3829
                     /locus_tag="IVY61_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7971552.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="IVY61_002485"
     CDS             4125..4406
                     /locus_tag="IVY61_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971553.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="IVY61_002486"
     CDS             4408..4824
                     /locus_tag="IVY61_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971554.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5438..>5779
                     /locus_tag="IVY61_002487"
     CDS             5438..>5779
                     /locus_tag="IVY61_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP7971555.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2154 a          982 c          947 g         1696 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcggttggt
     5281 acaggggcta cttggcatag tcgacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DACCXM010000141         5744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCXM010000141 DACCXM010000000
VERSION     DACCXM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.146"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(191..712)
                     /locus_tag="IVY61_002488"
     CDS             complement(191..712)
                     /locus_tag="IVY61_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP7971556.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(715..1557)
                     /locus_tag="IVY61_002489"
     CDS             complement(715..1557)
                     /locus_tag="IVY61_002489"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP7971557.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(1550..2275)
                     /locus_tag="IVY61_002490"
     CDS             complement(1550..2275)
                     /locus_tag="IVY61_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP7971558.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            2451..2747
                     /locus_tag="IVY61_002491"
     CDS             2451..2747
                     /locus_tag="IVY61_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971559.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            3315..4907
                     /locus_tag="IVY61_002492"
     CDS             3315..4907
                     /locus_tag="IVY61_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7971560.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            4920..5660
                     /locus_tag="IVY61_002493"
     CDS             4920..5660
                     /locus_tag="IVY61_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7971561.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         1638 a         1127 c         1160 g         1819 t
ORIGIN      
        1 aaaagctgca cttccctttt ttatgtttct ccactaatcg aaatttagat agacatctag
       61 aaatcgcaat tccaaaaaat tctgtgtatt ggatacgcat cttttcttat tttttcttat
      121 ttcttccata tgggtgttta tctcagagat gatagaatag tttctaggat aaaaagtagc
      181 agctacgatg ttatccttcg tagaggattc ctgcttcagc tagtttttgt ttgatgtttt
      241 tcagcatatg cttttctggg tagcggatcg tatgcagatg gatgccgcct gttaattcgg
      301 ataacaaagt agcattactt ttttgataac gtctcataaa ggaatcgata tcttgtggcg
      361 ttttgatgtg caaagttcct gctaactcac cgtaaagggg atgttccaca attacatcaa
      421 tgatttcacc accattttca acgattagtc tcagttcttc ttctgtttga tcttttccat
      481 gctggacagc gattttactg atgtgcccag tattggtttc ttctgtttcg aggatataac
      541 ctcttgctgt tgccacgata cttttacctg cagctcgtag taaagcaata tcgcctacaa
      601 tgatctgacg gctgactcca aaagttttcg caaacttgct agcactcact ggtttttctg
      661 tatttgccaa ggtttgcatg atttgttttc gccgtgtctc tccttcgatc acacttattc
      721 ctccttcatt ttgttttggt attcgcgcga gtgtttcatc aaatcaacat acgtgtctgt
      781 tattgtttcc ttatatccct tatttttcag cagactaaca actttatcca atacttgctg
      841 ctctcttttt gcatcataaa ctgcttggtt ttcttctttt ttcacttgtc ctattttagt
      901 taccgtatcc attctttttt ctaataaagc aaccaactct cgatcgatca tatcgatctt
      961 atgacgtaat gtttcaagag actcttctgt tagttcttcc tctactgtct tgatgatacg
     1021 agcaggattt cctgcaatca ctacattgtc gggaaaagat ttggtcacaa ccgaacctgc
     1081 gccgactact acgttgtccc ctagagtgac acccggcgtg acgataactc ctccgcctaa
     1141 ccaaacatta ttaccgattt taatgggttt tgcgtactcc agaccgctgt tacgtttcac
     1201 tggatgtagt ggatgcgtcg ccgtgtaaag ctggacattc ggagcgaaca tgcagttatc
     1261 accgatttca atcgtgctga catctaaaaa tgtacaattg aaatttgcat aaaaattctc
     1321 cccaacaaat atattatatc cgtaatcaaa attgataact ggctccatat agattttttt
     1381 ccctgtacgg ccaaaagtct ctttcaacaa ttgggatcgc agttccgctg attctgcttg
     1441 gttgatgatt tggctttgac ttcttgcaaa catacggtcg gcaaccagct caggatcatt
     1501 ggcaaaataa agctcgcctg caatcatttt ttctttttcg gttttgaatt cagtcataat
     1561 ctgctcctaa ttcttgtatt tttttgaaaa caaccgctcc gtcttcttga ataaacgtat
     1621 aaggctccgc taaataaaca ggcgtttgat tgattactaa gattttggct gacggctggc
     1681 gaaactgtac cagatcacaa aacggatgaa cttgaaaact cgttccaaca atgacgatca
     1741 gctctgcttg gctgacggca taagccgcct gctccatgac tccatcttga aacccttcac
     1801 catataacac gatgtctggg cgtatttgtc ctccgcatgc ttgatgtttg tcgctgtgaa
     1861 gatattcttt ccagtctacc gtttgttgac attttcgaca atgacattga tacagactcc
     1921 catgaaaatt gacgagttgc gggcttttag cctttgcgtg caaaccgtcg atgttttggg
     1981 aaacaaccca aacatttttt tctttcgcta gatgtgctat ttccaaatga atgatattgg
     2041 gttttgcttc aggatgataa atctttttga caaattgata gaatttatcc ggttcattca
     2101 ccattgcttg atgactcagc aagtattccg gctgctccat cccgtggtag attcctgaca
     2161 aagatcgata gtctggcact ccagaaggtg tggatacccc tgcaccggtc aaaaatgtaa
     2221 tttgctgata agctaatatc tcttttgctg cttcgtctac tgttaattgt ttcatgtttt
     2281 gtcacctcac tttccattat agcccatact cgaacaaatt tattgttgaa aagtcagaaa
     2341 aatgggaata aaaaagtgtt atgggcattt gagcaattta tcacaatgtt cactgacaaa
     2401 agaatgaaaa cgtgttacaa tactgctgta taaaagtgag gtgaatcgat atgttaccat
     2461 tctttattta ttggggcgtt attctggctt gtatcgcctg gttagcgtta agcgtttatt
     2521 tctctgtttt ttatcttgta cgcaaagaaa atggaaatct ttgggctttt gctttattca
     2581 acgtgattgc cgcaattgtt ttagccatta cattagccgt atatcgaaca tggggctggg
     2641 gaatcaccca atactcaagt ttgatttatc taatattagc catttatggc gtcgttgttc
     2701 ttttgcaagc gatcctagga cgtgagccaa agaaagcggc agcttaaatg atcgagatgg
     2761 aactggacac aggaaatttt ctgttctagt tcttttttta ttgtattaca tacatttttg
     2821 tgtataatta tactatcgtt tgcaatggat agatgattca cattttgatt gttttttcgg
     2881 taaaagtaaa gatttctata tttatcagta ttttttcaaa aaccagcagt tatagattga
     2941 caaagcctct cgagtatcgt aaactttttt tgtataaata actgaatacg agatgcctca
     3001 atcaatgagg tagaggtcgc gagaatcatt aaccctgtgg agtgaagcaa acatggagaa
     3061 gcaggaatag ggaggatcgc cgaaatgtgc acttttttgc ttggagtgta tgttgggacg
     3121 caagttaaga gcttgcggac tgtcttagca gtgatgctaa gttgcgctat gttttgtgag
     3181 aggtttatca ccttatggaa ttttagcggc ctttttatat ctataaatcg gtcgcttttt
     3241 attttttcag ataaagcttt accgcatttc atctagaccg ctcgtgtatc atataatcag
     3301 aaagggagaa aaaaatgaaa aaaaatatgg tttcagttgc cgtactattt gcttttgtct
     3361 tctcactatt cagtgtggga tctctggcaa atgcagatga aaaaacaccg agcgatgagt
     3421 ttcgcgtagg tatggaggct gggtatgcac ccttcaactg gtctcaacaa acagatgaga
     3481 ataatgcctt gccaattcaa ggccaaaata gttacgccgg cggttatgat gttcaaatcg
     3541 ctaaaaaagt agcagatggt ttagggaaaa aattagtcat cgtccaaacg aaatgggatg
     3601 gtctggctcc tgcccttcaa tcaggcaaga tcgacgcgat catcgccgga atgagcccta
     3661 ctgctgaacg acgaaaagaa attgatttta ccgatcctta ttacgaatca caattagtag
     3721 tagtagttca aaaagatagc aaatatgcaa aagcaaaaag tttagcagat ctttctggag
     3781 caaagatcac tgctcagtta aatacattcc actatactgt gatcgatcag ataccagatg
     3841 tgcagaaaca acaagcaatg gataatttct cagcaatgag aacagcttta gcttctggaa
     3901 tgatcgatgg ttatgtcagc gaacgtccgg aaggtgtaac ggcaactagt gtgaacaaag
     3961 acttggaaat gcttgaattc agcggtgcag atggcttcca gacaaacccg gaagatgttc
     4021 agatttctgt tggtatgcgt aaaggcgacc cggatatcca aaaagtaaac cagattcttt
     4081 ctggcatctc taacgatgaa cgaaccaaaa ttatggacca agcagtcaaa gatcagcctg
     4141 ctgcaactga tagtgaagaa aaacctggca tcatttctga tttcactacg atttggaaac
     4201 aatacggcaa tatgttctta cgaggtgctg gacttacctt gttcatcgca ttgatcggga
     4261 cagttgtcgg tactacactt ggattactga ttggtgtctt ccgttcggta cctgaatcag
     4321 gaaatgcact gactcgcttc ttccaaaaaa tcggaaatgc acttctttct atctatgtcg
     4381 aagtattccg cggaacaccg atgatggtac aagcaatggt catcttctac ggtctggcac
     4441 tcgcttttgg catttcattg aaccgaacga ttgccgcttt gtttattgtc tctctcaata
     4501 ctggagctta catgacagag atcgttcgtg gggggatttt tgcagtagat gaagggcagt
     4561 tcgaagctgc gcaagccatt ggtatgaccc atggccaaac gatgagaaaa gttgttattc
     4621 cacaagtttt acggaatatc ttgccagcaa caggaaatga gtttgttatc aatatcaaag
     4681 atacagcagt tttaagtgta atcggcgtag ctgatttgtt cttccaagga aactctgctt
     4741 ccggtgcgaa tttccaattc ttccaaacat ttacgatcgt cgggatcatc tacttagtca
     4801 tgacctttac gatcaccaga atcctgcggg cggtagaaaa gaaaatcgat ggtccttcag
     4861 cttataccaa gcttgaagaa atcgacaatt caaatttgca ggaataagga ggaagaatca
     4921 tgaatgaaaa aattattgaa atcaaacatc taagaaaaag cttcggtgaa aatgaagtat
     4981 tgaaagatat cagcgtaaca gtcaataaag gtgaagttgt gacgatcatc ggttcttccg
     5041 gttctggtaa atctactttc ttgcgctgta tcaatttact ggaaaaacca acaggcggcg
     5101 agatcattta taatggagaa aatgtattag ctcctaaata taatttacct aaatatcgaa
     5161 cgaatctagg tatggttttc caatccttca acctgttcaa caatatgaat gtcttggaaa
     5221 actgtatgtc tggtcaaaca actgtcctga aacgagataa agaaactgcc aaaaaagtag
     5281 caatcgaaaa tctggaaaaa gttggtatgg atcgttatat cgaagccaaa ccagcacaat
     5341 tatctggtgg acagaaacag cgtgtcgcaa ttgctcgggc attatcaatg aatccagatg
     5401 tattgctatt cgacgaacct acttcggcgt tagaccctga aatggttggc gaagtgctta
     5461 atacgatcaa agacttagca catactggct tgacaatgct tatcgtgacg catgagatgg
     5521 aatttgcaaa agatgtatct gaccgtgtca tttttatgga taaaggtgtg attgcagaag
     5581 aaggtacacc agaagatatc tttgtccatc cgaaagaaga acgtacaaaa gaattccttt
     5641 cacgtatttt aaatgcttag gatcaatagc tgattgggac aaagagctgc ggtgtttacc
     5701 gcagttcttt tttatgaatt actcataaaa ggtaaatttc tgaa
//
LOCUS       DACCXM010000142         5713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCXM010000142 DACCXM010000000
VERSION     DACCXM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.149"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            82..978
                     /locus_tag="IVY61_002494"
     CDS             82..978
                     /locus_tag="IVY61_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7971562.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1128..2069)
                     /locus_tag="IVY61_002495"
     CDS             complement(1128..2069)
                     /locus_tag="IVY61_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7971563.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2195..3316
                     /locus_tag="IVY61_002496"
     CDS             2195..3316
                     /locus_tag="IVY61_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7971564.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3574..5076
                     /locus_tag="IVY61_002497"
     CDS             3574..5076
                     /locus_tag="IVY61_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7971565.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5143..5670
                     /locus_tag="IVY61_002498"
     CDS             5143..5670
                     /locus_tag="IVY61_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7971566.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1870 a         1015 c         1211 g         1617 t
ORIGIN      
        1 tataggaaac gttttactgg ctttttacta tttaaacagg ctatcatagg aaaaagtgac
       61 agagaaagtg aggcggaaaa gatgaacaaa aatgacgtac tgctagttgc aaaaaataaa
      121 tcactttatt ttttgccttc catccgctct ttagatgttt ggtccggaaa agatttacta
      181 cagtcggaat tcattaaatc agctagacaa cagttggcaa attatcagct gatcgttttt
      241 ttagattatg gatttgatat ggagatggct cgacgcgtgc gcccatttac caatgcaaaa
      301 attgtgttat ttttttggaa tcacttcaaa gcagaacacg aggcgatgct gaagcaggca
      361 caagatgaac cggcaatcga tgagatttat cattttgatt ttttggaagc aaaagaactt
      421 gggctgaaac ataatagttc attttattcg aaacatatga atttttcttc tagcgaacca
      481 aagacggatc tattttttgg tgcaacgaat aatgggagaa aagaacgagc agaaagctat
      541 aagcaggaat ttataaaaag agatgttacg accaactatt tcattcttcc acatcgaggg
      601 aatgaacaac ctggatatct tacttatgaa gagtacctga aaaaaacagg ggaatcgcgc
      661 gggattctgg aactgttgcg ggagggtcag caaggagtga ctttgcggac atttgaatcc
      721 atgtattttc aaaagaaact agtgacggat aatcaagcga ttgctcatta tcatttttat
      781 cgtcctgaaa atattttttt gcttcgagaa cgtgatttag atgaattacc ggaatttctg
      841 catacaccat ttaagccaat cctatcagag ctattggaat tttatgatgc agaaaattgg
      901 gcaaaacgat ttatcactaa cgattccact atttatgagc aatacgaata tgacctctcc
      961 tctgttgaaa acaaataaaa aacctacatt ttgaagagat tatgtactga aacaaacaag
     1021 cacaattctt atggcatcat gagagttgtg ttttcttttt gatcttttat tgggcttaaa
     1081 aaaagagaac cagctgctga ttcagctgat tctcatcaga gagcctttta ttctgcgcca
     1141 aggattcctt cttttgcatg gactaattgg gtcaaaaagg cacaatatgg tgtagcaata
     1201 tcatattttt gtccttttcg ccaaatggca ccattgatgt agtcgatttc agtcaaacga
     1261 tggtttttga tcaggtcttg gtacatagat gggtaatgca acccgatgtt gtcaggatca
     1321 taagtagaag caatatgttg gtacacttct tcttgatcta gtatgattcc ttcttttgct
     1381 gcgatagaag caaattcaga tacgattgtt cgaaccatgc tctctgccgc tttttgcgcg
     1441 cctagttcag caatattaca atccaaaatc gtgcataaac cattcaatgt tccatttaca
     1501 caagcttttc gccaaatgga ataacgaacg ttatggctat agatcggatt taaatttgct
     1561 tcttgaaaga cttctacaac tttttttgta aatgcttcgc cttctggttc caagttctct
     1621 agttcgattt ctccatcacc taacaatgtg acttttccgg gaccagctag accagctgtc
     1681 cacatcgtga tcccaaataa gatatttttc tttggcacat atttttccag tacatcttca
     1741 tgacctaatc catttagtaa acacaaaaca taagtattgt cagtaataat ggattggatc
     1801 gaacaaaaca tatcatctaa ttgattggct tttgttaatg ctacgatcaa gtccacgtgt
     1861 tcgttgcttt cgatgatttc ttctggtgaa tagattggca gttttgcgac cacttcttta
     1921 ccgttaaagt cagcaatcaa cccatttgta cgaatggctt caatgtgagc aggccaacga
     1981 tcgatcaaga ggacctcatt tccgctttga tgtagcatca atccgattcg gctgcccatt
     2041 gctccggccc cagctattgc gattttcatg ttttttctct cctttattca atgagttccc
     2101 ttttagtata tagaaaaatg gaagaaaata gcattgtttt gtttgaaaaa aatgaaaaaa
     2161 agtgtcttac ttaagaagaa agtgagggat acagatggat attttggcgg caattgattc
     2221 atttaaagga agtgcttctt catttgaact aaatcaggca gttctaaatg gctgggaaga
     2281 tcgtaaagga agaaaaatca atgtgccgat tgcagatggc ggagaaaata ctgcagaagc
     2341 aatctattac gcgctcaggg gaacatggcg taccattgat gggatggatc tcttattccg
     2401 tcagcgaaaa atcagctatt tattgacaac ttggcaagga aaaaacaccg cagtcatcga
     2461 atctgcacaa gcattggggt tagatctact tgataaacca acggatcaaa ctatccgact
     2521 tgcttccagt tatgccttag gagaacttat taaagatgca ctgcttcaag aagtagaaca
     2581 aattattgtc actttaggag gaagcgggtg tagcgatggt ggtctaggat tactccaatc
     2641 cttgggtgct tctttagaag ggtacacaga aggaaatcct ttattatcta ctaaaaaact
     2701 tcattttgat cgaagaaacg ttcttgtttc taaagtaaaa ttgattatcg ctgcagatgt
     2761 taccaatcca tatacaggtc aacaaggagc tgcttggcaa tttggaagac aaaaaggagg
     2821 aatagatacg acgctagctt tcttagataa acaagcagga aagattgctc agcaaataaa
     2881 agatttctgg gaggtggatt tagatgatat tccaggaagt ggagcagcag gcggtattgg
     2941 tggagccctt tttttgttgg gagcgaagat ggtttctggt ttcgatttag tcagccaaat
     3001 cactggattg gaaaaaaaga tcgaacaagc agatgtcatc attacaggag aagggcggat
     3061 cgatcagcaa acgatccacg gaaaagttcc atacggtgta gcagtgcttg caaaaaaata
     3121 tcgaaaacca ataatcgcta tttgcggaag tagagaccaa gagctagaag gactttctga
     3181 ttttctccca attgtcttaa gtattcattt gggtccaata ggtttagaag aagcaatgga
     3241 acacaggcaa acattagaaa aaacaaaaat cttaggacag acacttagca gatttttcac
     3301 catcctcccg aagtagttga caaaatcatt ttctttatgc tatcgtaatt tcaaatatag
     3361 gacaaagtga gggatccgtg cattgaaaaa ttaagtcgat tgatgaaagc tgcattcaga
     3421 ctgtaccttg tggagacaag gaagcagtgt gcagattttc gggattggct tggttttttc
     3481 gatgtatggg tctttttgtg tgtatccaac ccttgcatcc tctttagcca gttctgaaaa
     3541 taggactggt ctttttttac gaggtgatgg aaaatgtcta aaatcgaaat aaaaaatctg
     3601 acattcggct acgacagcca aggcacatta ttatttgaac aagcaaatct aaattttgac
     3661 acacaatgga aactaggact tatcggacga aacggtcgag gaaagacaac tttactgaat
     3721 attctacaaa acaaactacc ttatcaaggg caagtaatcc atcagcaaga atttgcctat
     3781 ttcccgcaac agacaaaaga taaagaacgt ttaacctatt acgtgttaaa tgatattacg
     3841 gattttgaga tatgggaaat cgaaagagag ctccaattga tgcaaacaga tcctgaaatc
     3901 ttatggagag aattcagcac actatcgggg ggagagaaga caaaagtcct actggcactt
     3961 ttatttgtgg atgacactca tttcccgtta atcgatgaac caacgaatca tttggatatc
     4021 tctggtagaa aacaagtagc ggcttatttg aaaaagaaaa aacaaggctt catcgtggtc
     4081 agccatgacc ggggatttat cgatgaagta gtggaccatg ttttagcaat cgaaaaaagt
     4141 caactggaac tttatcaagg gaatttctct atctatgaag aacagaaaaa acttcgtgat
     4201 gaatttgaaa tggctcaaaa tgaaaaattg aaaaaagaag tcagtaggct aaagaaaaca
     4261 gcagctgaaa aagccgaatg gtctcgttcc cgagaaggag ataaaacaaa gaaacaagtc
     4321 ggattcatcg atactgaatc tagacgagtg aataaaggag cagtgggtgc tgatgctgca
     4381 cggacgatga aacgatccaa agcaatcgtg aatcggatgg agacccagat cagcgagaaa
     4441 gaaaaactat taaaagatat cgaatatatc gattctttga cgatgaatag ccaagcgtct
     4501 caccataagc gacttttaag cgtagaagat cttcaattag ggtatgaaaa tctgttattc
     4561 gagccaattc attttacaat cgagcctcat cagcgggtgg cgatttcagg tcctaacggt
     4621 gcaggaaagt catccattat ccattatctt ctgggggcat tcaacggcaa ggttatagga
     4681 gaaaaatacc agccaaaaca tctgagcatt agttatgcaa gccaaaatta tgaagacaat
     4741 cgaggaacgt tggcggaatt tgcagagaaa aaccaagtag actaccaagc atttttgaac
     4801 aacctccgaa agcttgggat ggaaagagat gtttttcata acaagatcga gcagatgagt
     4861 atgggccaac ggaaaaaagt ggaattggct aaatctttat cacagccagc tgaactatat
     4921 acatgggatg aaccattgaa ttatttggat gtcttcaatc aagaacaatt agaacaactg
     4981 atcttgaacg tgaaacctgc catgttacta gtggaacatg atcaaacctt cctggataaa
     5041 gtatctactg agattatttc tcttgagaga atctaaccct tgtaatctca agaacaacaa
     5101 ggtatggtta aggggaactt tgccaacgaa ggagagaatc acatgcttta tgtaacgaga
     5161 cacggagaaa caacttggaa cgcacaagga ttagtttgcg gacgggcaga tgtgccgctg
     5221 actgaaaaag gacagatgca ggcacagaaa ctagcagaaa aggtagtaga tttgcctgtt
     5281 ccaatcacga aaatcatcca ttcccctctt caacgagcaa gagatacagc acaagcagtt
     5341 gctgatcgtc tcagcttgcc gctgaccgtt gatgaacgat tggtagaaat ggattttggg
     5401 gattatgatg gactgccaag caaagatgag aactttcaaa aagcacgttt agcgttcgct
     5461 gtacgattcc caaatgggga gtcagtctta gatgtgtatg cacggatcgt tcctttattg
     5521 aaagaatgca tcgaagacga agaaaatgtc tatctgttag tttgccataa tgccttgata
     5581 agagtgatca atgcttattt tcatccgatg cctaatgagg ggttctttac attcatggta
     5641 gataatacag aattggtttc ttatgagtaa ttatgttttt gtgttattta aataaaatat
     5701 ttcaaggcac tgc
//
LOCUS       DACCXM010000143         5679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCXM010000143 DACCXM010000000
VERSION     DACCXM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.150"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(240..606)
                     /gene="ssrA"
                     /locus_tag="IVY61_002499"
     tmRNA           complement(240..606)
                     /gene="ssrA"
                     /locus_tag="IVY61_002499"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(777..1550)
                     /locus_tag="IVY61_002500"
     CDS             complement(777..1550)
                     /locus_tag="IVY61_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7971567.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1694..2044
                     /locus_tag="IVY61_002501"
     CDS             1694..2044
                     /locus_tag="IVY61_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7971568.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2025..2741
                     /locus_tag="IVY61_002502"
     CDS             2025..2741
                     /locus_tag="IVY61_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7971569.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2741..4189
                     /locus_tag="IVY61_002503"
     CDS             2741..4189
                     /locus_tag="IVY61_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7971570.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4260..4769)
                     /locus_tag="IVY61_002504"
     CDS             complement(4260..4769)
                     /locus_tag="IVY61_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971571.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4759..5484)
                     /locus_tag="IVY61_002505"
     CDS             complement(4759..5484)
                     /locus_tag="IVY61_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971572.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1936 a          984 c         1120 g         1639 t
ORIGIN      
        1 gtttttcctt ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt
       61 ttgtaaagta ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt
      121 tttgaatgct attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa
      181 atgctcaaac ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac
      241 aatggagacg gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt
      301 catagtcaac tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc
      361 gctagtctga tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa
      421 gttaggaccc ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta
      481 ggcagctaaa gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt
      541 tacgtagacg taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac
      601 gtccccgtta gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat
      661 tgggataaat tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt
      721 tcttaagaaa aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa
      781 atcagatcaa aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata
      841 aaggtagaaa gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa
      901 gcttccaaca aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc
      961 agctgaagtt ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact
     1021 gtatcgatcg agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag
     1081 tattcgtcac ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct
     1141 aaaggatcaa tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg
     1201 ctatgaccgg tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac
     1261 tgtacatgat catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct
     1321 tcgccgtcta ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct
     1381 tccatgatag agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg
     1441 ttttctttta atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct
     1501 agtaatgttc catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact
     1561 ccttttttat tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg
     1621 aagaattcct gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt
     1681 taggagggaa agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct
     1741 ttttcggatt ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc
     1801 cgcaaaaaca gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct
     1861 tatgggctta ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta
     1921 ttttctgatt gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga
     1981 aaagaaatta cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga
     2041 ttgaagataa tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg
     2101 aagcaacttt caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac
     2161 attgggatat gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa
     2221 gagagatccg caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg
     2281 aaagcgatca ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca
     2341 gtcctctgac gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag
     2401 caagagaaga agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga
     2461 tgaatacgaa aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta
     2521 aagagttcga tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc
     2581 agttgcttga attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc
     2641 atatcaagaa attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt
     2701 ggggagtcgg ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca
     2761 actcttagct ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac
     2821 acaactgaca aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga
     2881 atcagttgaa aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca
     2941 ggcgtttcaa aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt
     3001 tatatttatc gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt
     3061 taccttaaca gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc
     3121 gaataaaaat attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt
     3181 gaatcatgaa ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag
     3241 cgttcgtcca attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt
     3301 tattgccagc tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc
     3361 gacgaaagaa gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga
     3421 atttgatgag ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc
     3481 agaaattgaa gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat
     3541 gagaacacct cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc
     3601 tgaaaatcag cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg
     3661 tttggtgaat gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat
     3721 taaaaaattc aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc
     3781 agaagcagca ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga
     3841 ttatgaccga tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga
     3901 aaacggccaa atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga
     3961 agatacggga attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa
     4021 agtagatcct tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt
     4081 gcagcaactt gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg
     4141 aacaaaattc gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg
     4201 tataaaagat catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt
     4261 tagcccgcgg gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta
     4321 aagagtaaga taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct
     4381 tggatctcca atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg
     4441 gaaagcaagg catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc
     4501 gcacaattaa gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt
     4561 ttgatttctt tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac
     4621 atcggccaat ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt
     4681 ttcaagtgct ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt
     4741 tcagcatgaa tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg
     4801 gataaaaaaa gtgaaaggga atagactaag aatgagacag catccactga gcccaataag
     4861 agtttgggca aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt
     4921 aaaaaaagtc tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg
     4981 gatatctggc agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc
     5041 ataaactgac tggaagctta cgatccacaa taaaaccaag atacaacaaa gaaagagagg
     5101 aaagaacagc tgccagaaaa taaaaaggaa taatttggtt ttagaaagac cagtcaggtg
     5161 ccaattgatc aattcctgct tgttttgcct gactgaatag taaaagaaaa agataaaaag
     5221 gaaggcaaat agaaaaaata aactgaatat gccttttata taaaatcggc tagctaattc
     5281 taaagccgga agaagctctg tagatgcaga agcgccaata aatgaaagcc gatcttggac
     5341 ttgattggct aaagccgatt gcaaatggta aagagtcagg catgctatca ataaaaaagc
     5401 agaaaagctt gaaaataaac aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc
     5461 agtagcatag cggattattt tcatactttt cacctctata aaaagtatag aaaatgaata
     5521 tgaataaagt atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa atattttgta
     5581 aaaagcaaga ggttgtgaag aagctgccct attctattac cgtagatgca agcttttgaa
     5641 cactgtttat tcgaaaagag gagcagcgac aataatttt
//
LOCUS       DACCXM010000144         5677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCXM010000144 DACCXM010000000
VERSION     DACCXM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.151"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(24..482)
                     /locus_tag="IVY61_002506"
     CDS             complement(24..482)
                     /locus_tag="IVY61_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7971573.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(495..1820)
                     /locus_tag="IVY61_002507"
     CDS             complement(495..1820)
                     /locus_tag="IVY61_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP7971574.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1837..3249)
                     /locus_tag="IVY61_002508"
     CDS             complement(1837..3249)
                     /locus_tag="IVY61_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7971575.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3266..4030)
                     /locus_tag="IVY61_002509"
     CDS             complement(3266..4030)
                     /locus_tag="IVY61_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP7971576.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4134..5009)
                     /locus_tag="IVY61_002510"
     CDS             complement(4134..5009)
                     /locus_tag="IVY61_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7971577.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5264..5348)
                     /locus_tag="IVY61_002511"
     tRNA            complement(5264..5348)
                     /locus_tag="IVY61_002511"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5312..5314),aa:Leu,seq:caa)
     gene            complement(5366..5437)
                     /locus_tag="IVY61_002512"
     tRNA            complement(5366..5437)
                     /locus_tag="IVY61_002512"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5403..5405),aa:Gln,seq:ttg)
     gene            complement(5447..5519)
                     /locus_tag="IVY61_002513"
     tRNA            complement(5447..5519)
                     /locus_tag="IVY61_002513"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5484..5486),aa:His,seq:gtg)
     gene            complement(5581..5665)
                     /locus_tag="IVY61_002514"
     tRNA            complement(5581..5665)
                     /locus_tag="IVY61_002514"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5629..5631),aa:Leu,seq:caa)
BASE COUNT         1716 a         1216 c          869 g         1876 t
ORIGIN      
        1 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
       61 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
      121 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
      181 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
      241 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
      301 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
      361 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
      421 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
      481 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
      541 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
      601 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
      661 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
      721 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
      781 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
      841 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
      901 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
      961 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
     1021 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
     1081 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
     1141 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
     1201 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
     1261 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
     1321 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
     1381 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
     1441 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
     1501 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
     1561 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
     1621 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
     1681 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
     1741 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
     1801 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
     1861 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
     1921 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
     1981 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
     2041 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
     2101 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
     2161 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
     2221 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
     2281 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
     2341 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
     2401 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
     2461 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
     2521 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
     2581 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
     2641 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
     2701 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
     2761 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
     2821 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
     2881 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
     2941 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
     3001 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
     3061 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
     3121 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
     3181 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
     3241 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
     3301 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
     3361 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
     3421 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
     3481 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
     3541 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
     3601 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
     3661 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
     3721 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
     3781 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
     3841 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
     3901 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
     3961 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
     4021 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
     4081 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
     4141 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
     4201 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
     4261 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
     4321 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
     4381 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
     4441 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
     4501 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
     4561 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
     4621 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
     4681 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
     4741 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
     4801 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
     4861 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
     4921 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
     4981 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
     5041 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
     5101 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
     5161 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
     5221 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
     5281 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
     5341 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
     5401 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
     5461 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
     5521 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
     5581 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
     5641 cgtctgccaa ttccgccacg ccggcaagtt gatttat
//
LOCUS       DACCXM010000145         5589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCXM010000145 DACCXM010000000
VERSION     DACCXM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.152"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..372
                     /locus_tag="IVY61_002515"
     CDS             <1..372
                     /locus_tag="IVY61_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP7971578.1"
                     /translation="DAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIV
                     PQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKAD
                     YVTVSNDKDGVSQAFERILTI"
     gene            653..1873
                     /locus_tag="IVY61_002516"
     CDS             653..1873
                     /locus_tag="IVY61_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP7971579.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1870..4590
                     /locus_tag="IVY61_002517"
     CDS             1870..4590
                     /locus_tag="IVY61_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7971580.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4574..5518
                     /locus_tag="IVY61_002518"
     CDS             4574..5518
                     /locus_tag="IVY61_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7971581.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2073 a          899 c         1100 g         1517 t
ORIGIN      
        1 gatgctatca gcatttacaa attttttatt tttattccta atcgtcaatt gaaagctaat
       61 atatttaacc atttgaaaaa gatgctaaat gtgtctgtta ctgaatcggc acctgtaaat
      121 attgaaatcg tacctcaaaa tgtatctaaa ggtgtggtgt ttgatcgttt ggaatcaatc
      181 tacaatttaa agaaaccttt gagaatcgcg atcggagata gtttaaatga cttatctatg
      241 tttgaaagtg cagatattag ttttgcaatg ggaaatagtc atcaagaaat catgaaaaaa
      301 gctgattatg tcactgtttc aaatgataaa gacggagtat ctcaagcatt tgaaagaatt
      361 cttaccatat aaactaatta aggtagtata gaacatagaa ttctaaatac ggaaaaatgg
      421 agcataaatt ccagtacaaa tagtacttct tcagtgtcaa acgaagcgaa tataacatag
      481 atatttagga ttgtgacaaa aatcatgtca caatcttttt ttcgaataaa cttatttttt
      541 gaagtcggcc atttatcgta atttctttgc ataatacgaa caaattttcg gttattgatg
      601 aaaaaaaaca ttgaaaatgg tatgataaaa gaaaaaaagg aggaaatttt taatggtgaa
      661 atttatccat gcggcagatt tacatatgga tcggtcattt gaaggactga caactcttga
      721 taaaactgta caggaaaaac tattgaagac gaatctgacc gttttatcaa atatcattga
      781 acaggcaata ataaataacg ttgattttgt tttacttgca ggagataatt ttcatcaaaa
      841 tcgtccttca ttaaaaatac agaaacattt ttcagaacag atgaaacggt tagaaaagaa
      901 ccatatacct gtatttataa tattcggaaa tcatgatttc tatcaaaaag agcggtattg
      961 gttctccttt cctaaaaatg tccatttatt tacatccgaa acggtagaga caaaaaaaat
     1021 aacgataaaa agtggagaaa ctgtctctct cagtggattt agttatcgtc aaccgtggat
     1081 tcaaaaagac aaagtgatgg aatttccttc tagagaactc acagattacc atattgggtt
     1141 gtaccacggg gaacctggcg tctctcaaaa aggaaactat gctcctttcc aaccctcaaa
     1201 gatgcaagaa aagggatacg attattgggc tttagggcat atccatgtgc caacggtctt
     1261 aaatgaaaag caaacaattg tttatccagg tgcgcctcaa ggtcatacta aaaaggaaca
     1321 agcttctaca tctattctac tagttgaatt aagtaaagga agttgccaaa tacatccgat
     1381 taaagtagca gaggtctatt ggaagacaaa ggaaatatca ttgaggaaag caagaacgac
     1441 aaaagaggtc attgttcaca ttcgtcagca gctttcaaaa gtagaggatg gatttagttt
     1501 aattgagata aaattaaaag attatgacca tctcaataca gatgttttgg aacggataca
     1561 atcaggagag ctattagact atttgttaga ggaattctca gataggataa atgatttctt
     1621 tatttggcgt atttctctca tagagaatac tttttatacg aagaccccac ttgctgcttc
     1681 tgctaaattg atggaacagt tatttcaata ctatcagaca cctcaagatt tccaacaaat
     1741 tttaaatgaa gtatatagtc atcaagaagc ggctagaata ttgagcgaac tgccggaata
     1801 tcaagaagaa acacttgaaa aagccaagca gctgcttaat caagattttt tatttgagga
     1861 ggatcaggaa tgaagatttt aaggatcgag attgctgctt ttggaaaatg gcgtcaaaaa
     1921 agttttgact tttattcagg caatcagctg atctatgggg gaaacgaagc aggaaaatca
     1981 acgatttatc aatttattca agcaatatta tttggtttcc cttcaaaagg ccgtaaaaaa
     2041 aaggattata caccaaaaga cggctcagcc tatggcggca aaatatggct taaacatcct
     2101 gtatatggtg aatttgctgt cgagcgctac aagcagcaaa atcgcggaaa atcgaaggtg
     2161 tggctggggg atcaagtggg aagtgatgag ttactagaaa aactgatcgc acctttgacg
     2221 aaagagttat tccagcaggt atttactttc cagcaagagc agttgatgga actagatcat
     2281 ttgagagaaa aagagctaca tgatgcgctg atttctttag gcatcacggg cagttctcaa
     2341 gtattccaaa agcggcaaga atattatcag aaagcacaac aattattcaa gaaaaaaggc
     2401 caaaaattac taatcaatca aaaattaaat gaatggaaag agctgcaaga aaaaattcat
     2461 caaaaacaag aacaggaagc ttattttggc aagttgatcc aacaagaaca ggaatacgag
     2521 tcgcaacagc agcagttatt ggcacaatta aaagaggcga acaaacaatt ttcattagcc
     2581 agacagcaag aacttaattt ttctttatat gaagaatggc aatctctcaa aaaaaacagc
     2641 actacaggtc ttcctgatga gcaacttctt ttagatctgg agtctttctc caaacggtat
     2701 cagcaaacaa atgagcgttt ggaagaatta gaagcaacac tggaaaagca tagcgggatg
     2761 gatcagcagt cagccagata ttatttttat ttagagcagg agcaggaact aaaagatctc
     2821 cagcaacttc aatttacagc ggaacaatta aacgaagaat ggcaaagatt acaaattgag
     2881 actagagaaa caaattcaga attgaagatt ttggaacaaa ggtggcattg gcagaaaaat
     2941 ccgttaccgg aagaattttt atatgaagaa gaatggaagc atcttctggc tgaagaaaga
     3001 aggattgctg atctagaaag ccagatttct gtgcgtctcc aaatagctaa agaacagcaa
     3061 gagatgattg aagcagaagt gaatgacttg gaagagacct atccagcatt attacaaaat
     3121 cagtcagaga tagcaccagt ctcttcatct aaaccgtata tgctgatggc tggcggagca
     3181 ataatcattg caggtatatt tgtaccaatg cccttgaaag tgatcctttt actaattggt
     3241 gcaggcatga ttggctattt cttctacaaa aggcaaccaa aaattaaaac taaaaatgat
     3301 ttctctgaga taaaaggaat gtggcaagaa aaactaggac aacttgatct agccttagct
     3361 caattagctg aagcagaaga aaaacaaaag agaactggag aagaaaaaca gattcttcag
     3421 caagaagtat atcgttttgc ggatcatcgg cacttaggaa agatggatac gctcaaactc
     3481 atgaaagaat atggacagga agtcatagat tatcagaaat tatatggcgt atttgagcgg
     3541 aaacataaaa gagaacagga aatacaagaa aaactacgac aaatcgacca aaaatttgag
     3601 tttgttaaag aatggttacc gatacaagat aagacaattt cagaaaaaat ggtattgctt
     3661 tcccgtttta tacaagagat ggaacagatg aaatttgcca gaagctatca gcaaaatact
     3721 ttattgaagc aagaaatcaa tcaattgaaa aagaaacaaa aagtatggat acaagaatac
     3781 agcgaacaat tgactcaagc aggcttatct tatccttcgg aagtacctgt ctttttacaa
     3841 aaagccaaac aagccgaaca gcagatgaaa agatacaatg aattatcgga aatgcttact
     3901 ccactatttc ctgaagagct gacaaaagag aaattatctg taagactgcg ccaactgaaa
     3961 gaaaagcaat cccaactcca gcaagaagaa aaagaagcaa gtgaaaatcg gcaacgactc
     4021 caacttcaag tagaagagtt gcaaagagac ggcacattgg acgaacttta tcaacaagct
     4081 agccggcaaa aagcaaaatt acaggaatta ctggaggaat ggggagcact tgaaatcgcc
     4141 gggacattct taggtgatct ttcaacagag atgagtgaaa gacaattacc ccagttgtta
     4201 agagcagcag gtgattattt aggtatttta acaaaaggaa attatcagaa gatattactg
     4261 aaaaatgata cactgacttt aacagacgga tatacagaat ttcccatcta tgagttatct
     4321 acaggtacga aagatcagct gattatggcg atgagatttg cttacctctc attagaagct
     4381 aaccgttcga tatgcccgat catcattgat gatggctggc ttcattatga tcatcagcga
     4441 aagtatcaat tagctgaact tttagctaaa tttggagaaa agtatcaaat aatttgtttt
     4501 tcttctgata aagaaatggt aagctattac catgagatga accaaatcgt aaaacaatta
     4561 gaaggagcaa ataatgaaaa aaattcgtga tttagtaatc gatgagcagt ttgaacaatt
     4621 tgtcctgatc aaaagtgcag atgtaagaat cgctaaaaac ggaaaaaaat ttattgcttt
     4681 tactttccaa gatacttcag gtactatcga tggtaaatac tgggatgctt cggaggagga
     4741 aatcaataaa tttacagccg gcacagtcgt ttttctaaac ggtaaaagag aggtttatca
     4801 aggaaaccct caagtaaaga ttcttcatat gcgcttaaca agggtagatg agccaggaga
     4861 gcctagttta tacatggaaa gagccccttt gaagaaagaa gaaatggaag aagaaatcaa
     4921 tcaattgttg tttgagatca ccaatgcgca ttggaatcgt attgttcgct atctacttaa
     4981 taaatatcaa aaacaatttt ttgagtttcc ggcagcaaaa agaaaccatc atgcatttgc
     5041 cagtggatta gcttatcata ctttgacaat gcttcactta gggaaatcaa tcgccgatga
     5101 atatcctgag ctgaatcgag cattgttgta tgctggtatc attttgcatg atttaggaaa
     5161 ggtgattgaa ctttccggtc ctatgtcgac agaatatacg cttgcaggaa atttgatcgg
     5221 tcatctggta ttgattgacg aagaaataac taaagcctgt cttgcgttga aaatcagcga
     5281 agctgacgaa gatgttctcg tattacgtca tatggtttta gcacaccatg gcttattaga
     5341 atatggctca cctgtacgtc caagaataat ggaagcagaa atattacatc aaatcgacaa
     5401 tatcgatgct tcgatgcaga tgatgttgac ttcgattcgt caaacagaac caggaaaata
     5461 caccgatcgg attttcggta tggacaaccg aagtttctat gtaccaaaag atttatagca
     5521 aaaatttatt tttttcattc aaaacaaaaa aagcgagaca taagtcttcg ctttttttgt
     5581 tttctttta
//
LOCUS       DACCXM010000146         5456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCXM010000146 DACCXM010000000
VERSION     DACCXM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.154"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..275)
                     /locus_tag="IVY61_002519"
     CDS             complement(<1..275)
                     /locus_tag="IVY61_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7971582.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVE"
     gene            complement(275..580)
                     /locus_tag="IVY61_002520"
     CDS             complement(275..580)
                     /locus_tag="IVY61_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971583.1"
                     /translation="MSVGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(577..738)
                     /locus_tag="IVY61_002521"
     CDS             complement(577..738)
                     /locus_tag="IVY61_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7971584.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            complement(753..1112)
                     /locus_tag="IVY61_002522"
     CDS             complement(753..1112)
                     /locus_tag="IVY61_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP7971585.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(1109..1960)
                     /locus_tag="IVY61_002523"
     CDS             complement(1109..1960)
                     /locus_tag="IVY61_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7971586.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1977..2810)
                     /locus_tag="IVY61_002524"
     CDS             complement(1977..2810)
                     /locus_tag="IVY61_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HAP7971587.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(2817..3503)
                     /locus_tag="IVY61_002525"
     CDS             complement(2817..3503)
                     /locus_tag="IVY61_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971588.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(3509..4255)
                     /locus_tag="IVY61_002526"
     CDS             complement(3509..4255)
                     /locus_tag="IVY61_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7971589.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            complement(4248..4589)
                     /locus_tag="IVY61_002527"
     CDS             complement(4248..4589)
                     /locus_tag="IVY61_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971590.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(4768..>5456)
                     /locus_tag="IVY61_002528"
     CDS             complement(4768..>5456)
                     /locus_tag="IVY61_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7971591.1"
                     /translation="KNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAENWADLFWE
                     DTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKELKDQLPS
                     IPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMVRNKIRVI
                     CNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMPSTATMTI
                     IKQMELLEDE"
BASE COUNT         1354 a         1179 c          845 g         2078 t
ORIGIN      
        1 ttttctacaa atttgaaacg atcctgatga agtttcttgc tccaatttgt ttgccgatcc
       61 agctcacgca tctgatcgaa ccctttttga atttcgttgt aataaaattc aatgtttgct
      121 gctgctttcc aatgcctgct acttcgcact cctgctcctg tttcagccat ttccaactta
      181 actaattccg ctcgttcttt tgctttttta tctttctgaa tcttcatcat gattttcttg
      241 aggatgatat cactgtattg tgtaatgaga tccattattt ctcctccaca tacctaaact
      301 gtcgcccctt tgaatcaatc cataagctcc tagctctatc ccagatgatg tttttgctca
      361 gtccagtaat ttcagataac tgttcagctg tacccgttac tagaattcga tcaccatgcc
      421 agattgcaat ttttctcggc gttttccgtt taggcttttc agtccacatt gatttaccga
      481 gcttttggac ttctgcaact atttctttgt cttcttgcca attctcagaa tgtgtcagtt
      541 caatgattcg tttcattgct gctttcttac ccacgctcat tcctccaatc tacgaatttc
      601 ccttcttaaa ttcactatgt gcaaattgat tgcctttctc gccgtttcat tgaccatcac
      661 tgcctttatc cgttccagat cgtcaatctc acgttgaagg cttcgaatac gcatttgaat
      721 cacttcttct gttgtcatga tggaccacct cgttaaaaac gctcttcctt gaacgtattc
      781 cgatattttt tagctaatat caacggcact tgatattgat gacagaacaa ctttgccttg
      841 atcttaaagt cttttgtctg cattcctttg acatctacga ctttgacaag tttgccgttt
      901 ttataaaatg tgaagtcggg aatatactcg atcttgcgat acttttttcc atctagttca
      961 aatttcggca tcagctcaaa tctttcctga agttttactt tccagccgtt cgcttctgct
     1021 tgccacaagg ctaaatcgta atactctgct tccgcgatag aatcaaactt gatacctcga
     1081 tgaacagttt tcttattacg gtatttattc atgcgatact acctttcact ggttttatgc
     1141 gcttgtctgc tgtttgttgg aatttcagcg cataaccttc tgaattctta aatattctag
     1201 aaacaattct ttcgccgtag gcttctctta gttcaggacc ggataagttt gttgtgatga
     1261 tcgttgcctt gttctgtctg gcttctaaga gcgtgtttaa cgtgttgttt gtaaactgcc
     1321 tactatttga taccccgcta cctaattcag ctccaatatc gtcaaaaacc accaaatcag
     1381 ttgttttgat atcggctata agcgatcctt caatttcttt tctcagttca gcattgttat
     1441 aagaaaactt tatttgctct aataactctt gatagcttat aaaaagtatt ttcttgtcat
     1501 aatttgagcg ctcaagtatt tcccaagctg tcgccattga caagtggctt tttccgcttc
     1561 ctgatttccc tgatagaatg aaatgtgcag gatggttcag taagacatca tttacaaagc
     1621 ttttagctct ttctaaagca attttcgttt cttggtccac tacgtgataa ttctccattt
     1681 tgcatttaaa caaagtttta tctgttaata ccgaaccatt ttgaaaaaaa ctcaacgctc
     1741 gtgcttttaa gctgtcgtta tatatccgtt cggtctgtat atcctctttc acacgtaacg
     1801 ctttataacc acaactcatg catgttggtt tacaacgttc tgaaccatcc ttatttttag
     1861 ctcgccaact atacaaaggt tcgctacatt ctggacattt cccgctttgc actaatactc
     1921 ttcttattag tttctccata gcatttgcta ggctttccat gtgatgcatc tcctttttaa
     1981 attggcaagt cgtcatattc actaggattg ctgtactgta gtttttgact ttgcttttta
     2041 tgattattct tgtctgcttt gatttcgaat ttgagcttct caaatttttc tctcaatttc
     2101 ttagcacttc taatatttcc aaaccaaaat tcatttgtag gtagccaatt gatcacatac
     2161 tcaatcgctt ctatagacgc tttatctctt tcttccatca acctgattgt gtctgcccat
     2221 ttttcgatat ctactttgtt catttctttt ggaaaatctt cagttaaatt actttgcaat
     2281 tttttagcaa ggcgtaagtg ttcgtcagaa tacttacctt tcttttcttc tttatctata
     2341 tctatatctt tctctatctc tatctctaac tctggtgtag ttttgtctgg acatttgtcc
     2401 gacacttgtc ctccagttat taaattccgt tttgcctctt ctattttctt tctgtattct
     2461 ctttttctat ctgcttcagt tgaggatttt ccaatgaaac tttgtatatc agacatataa
     2521 attgctccgt tatctaatac gtcaataagc tgcaaatcac ggaaaatttg taccgctttt
     2581 tctacgactc ctacagaatg tcttgtaata gttgcgagca ttgtagagtt aaatggaatc
     2641 ctgtcattaa acatcaactt accttcgtgt tttagacttc ttaaataaag tttgagaaga
     2701 atattagaat aaatatagcc atctggcata ctttctaaga gaaccatctc gtcactatcg
     2761 aaaaaattct cttttagttt taaatagtag tagcgtttgt tgtcagacaa tattttttac
     2821 cctcctattc taagtttctt aattgtttcc tggtttaact tgatcccttt gatttgatat
     2881 ttatttttga aattgatcac acctatattg tgtttctccg tgtgatgaat cctgcagagt
     2941 gctgcaaatg tgtactctga atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
     3001 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
     3061 catttgtaga agtaatattc ttgattcgct ggtaaaatct catagccttc tttgaaagga
     3121 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattagc atcactcaca
     3181 gttgtattcg attcgtcttt gaggcttatt ttgcgccctg tgacgccttc aaaacggaag
     3241 taaaagaatt ccttccagaa gtccgttggc atgcctgtat cgatgaaaat atcgcctatc
     3301 agcgcataga tgaagtttcg ttgctgcaca gtaaatcgac gtggatcaat aaatcgaact
     3361 tcaataatcc gatcgccatc atatccgtca tacatcgtct ttagtcgatc aatgttcact
     3421 tcttcattga tcgttgcacc tatgtctttt cctttgaact ttttcagaac cgctgaatat
     3481 gaatcgatca atggtttaaa cactcatatc acttctcttt tgtttcttct ctgtactgat
     3541 cttcaatcca gttaacgcct cgttttagaa tgcccaagtc tctcttggtc catttactgt
     3601 catcagcggt tatagaagcc gcatcagtca atgcaacaat tgcttcatca attgattttt
     3661 cgtacttgtt agcaaccagt tgtaaagcat ccaagaatag ctttttgctt ctttgagtag
     3721 ctggttcaag catcgagaca tcttctggca tatcttcgcc agcaaatata tatagcccta
     3781 gcccaaacat tgctagattt tttacaagac agcgcatgat cgttttgttg atatcaaaca
     3841 tagttgctgc ttcaactcgc ttttcgattt ttccaacaat ctcttttttc ttcgtttcgt
     3901 tattccactg ataatcattg acttcgtagg tatatggctc atctttcatt gccttgtttg
     3961 caccatccat gactggtaac cacatgtcac gctttactcc gttgactgtg atactggtaa
     4021 aaaccatata gcctgttttc ttatcaaaga catacggcag atatgtttct ggatcatgat
     4081 agatttcata atctactgcc tcacacactt tgctaacttc tgcccaagcc catgcccacg
     4141 aaagataagt gaggttattt cgtttttcag taacatcttt cacatttatt ttgtataagg
     4201 tattaaaaag cgtattatca cttctatttt tcaaaggttg ttccttttca ctcatcaaat
     4261 tctgcctcca tttcagcaat gtatttctta cctgatccgt aataagagat atcaatcaag
     4321 ttatctctgt catactcttc tagcgcatca atcaagccat cttcgatgac atagatatat
     4381 tcagtttttt tggacttcct cgataaatgg ataagataga catgatccca aatactcaca
     4441 aaatttccca aatcgtcttg atcacatgct agttcttcat ccgtcaaaag attacgtctg
     4501 atttttcgat tgcttgtttc cttgatattc gatttgcccc aactaggatc agtcaaatat
     4561 tgatctagag tggaaagttc ttttttcatg tggtaacatc tccttagatg tattttcttt
     4621 gtgactcatt gctttggtcg gctgagtcac ttttttattt gttgccatgc tttttgctta
     4681 tcaatatgtt gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt
     4741 attacgccta tttttagcgc ttcagctcta ttcatcctct aacaactcca tttgcttaat
     4801 aattgtcatc gtcgctgtag aaggcatcca attgttaata aaatcgataa catcatcgaa
     4861 ctgttttgcc ttgatacgat ttctagctac tgctccagtg attcgcttaa ttccgccatt
     4921 caagtcttta aacaattctg cttttgcttt gctatttaaa tgctgctgat tacaaataac
     4981 tctaattttg ttgcgaacca tattagaaat tgttcggtaa tctggttcag ctagaagttg
     5041 attgttcttc aaatcgttta aatcagactc gatagcgtct actctttcat tcgtttcttc
     5101 gtttgcagct agagcaagta atgccatttc tcgtttagtt gctggaattg atggcaattg
     5161 gtcttttaat tctttttcca tcttgttgaa ggcctcaata tattgaagtt taaaacttaa
     5221 agctttctta ccagtgaagc ccattgctaa tagagagaag ccgtctctat tcatataaat
     5281 aattcggtat gattgtttat tttgaggatg aacataagta tcttcccaaa ataggtctgc
     5341 ccaattttcg gcaaccccct cttttagttc atcaatagcc gtcaaaacat gtctgtgctt
     5401 tttatcgaag ctttctgcaa cttgtaagct cgtagtcaca gcttctttat ttttca
//
LOCUS       DACCXM010000147         5241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCXM010000147 DACCXM010000000
VERSION     DACCXM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.155"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..497)
                     /locus_tag="IVY61_002529"
     CDS             complement(<1..497)
                     /locus_tag="IVY61_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP7971592.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
     gene            complement(581..796)
                     /locus_tag="IVY61_002530"
     CDS             complement(581..796)
                     /locus_tag="IVY61_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971593.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIESIKYFV"
     gene            complement(806..1621)
                     /locus_tag="IVY61_002531"
     CDS             complement(806..1621)
                     /locus_tag="IVY61_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7971594.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1883..2326)
                     /locus_tag="IVY61_002532"
     CDS             complement(1883..2326)
                     /locus_tag="IVY61_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP7971595.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2460..2798)
                     /locus_tag="IVY61_002533"
     CDS             complement(2460..2798)
                     /locus_tag="IVY61_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7971596.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2786..3163)
                     /locus_tag="IVY61_002534"
     CDS             complement(2786..3163)
                     /locus_tag="IVY61_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7971597.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3387..4580
                     /locus_tag="IVY61_002535"
     CDS             3387..4580
                     /locus_tag="IVY61_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7971598.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4746..5168)
                     /locus_tag="IVY61_002536"
     CDS             complement(4746..5168)
                     /locus_tag="IVY61_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7971599.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1525 a          984 c          923 g         1809 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctcgatgca acctggacca ataaaaagta aagaaaaaat aaaggaaaag acattccttc
      661 catctatcga caaaaggaac gcagctgcta tatacaagat attgacacac caaaaacaaa
      721 ggctacttgc caatgattga tacaaccaat aaaagaattc catataaaca tttttgtcaa
      781 aacgctgctc tttcataatc acctcctatt ccttcattcc tgatgaagcg atcccttcta
      841 caaaatattt ttgcatcgct aaaaatataa aagcaacagg aagaacagaa acaactgagg
      901 cagccatgat taatgcgtac tctgcatcaa atagcccaat aaacattttc aaccctaact
      961 ggattgtttt attttccgta gaagacaaat aaatgaatgg ccccatataa tcgttccatg
     1021 tattaacgaa tgtgatgatc gtcaaactgg caatcgccgg ttttgtaaga ggtaaaatga
     1081 tcttgcgata gattccccac tctgataagc catcgatccg cgcactctca cataaagaat
     1141 ctgggatact actgtagtat tgcttcaaca agaacacacc aaaagcattg aacgcttgta
     1201 atagcaccaa cgaccacaac gtatatgtta aacccacttt tctcatcata atgaactgcg
     1261 ggatcatata agactgccaa gggacagcaa tcgtgccgat atatgctaaa aataaaatat
     1321 cgcgcccttt aaaatttagt ttagaaaacc cgtaagcagc aaaactagaa gtaaacaact
     1381 gaatcaacgt gatcacaacg cttaaaaaag ctgtattctt aaaaaatgtc actaaaggaa
     1441 tacgagacca aataaccaga taattctcta aatgccaagt tttagggatc cattgaatcg
     1501 gaatcgtgaa cacttcatta tttgctttga atgaagcaga aaccatccat aaaaaaggaa
     1561 tgatcgtcaa taaagctaat acaatcagca gaatcatacc caaccattga ataaagggca
     1621 tgttttttga acgaattttt gctttttttc tttcatttcg aacacctcca aagttataat
     1681 cttcatttct ttgtaatgat tacaatccag aacaacggtt attatagctg attcatttcc
     1741 gttcttctta tgtttgtaag cgcttattta acaagttatg taaatctctt tatttttttt
     1801 gatttaaaaa tgaaaaaata caaaagcaag actgtcatac gtctcgcttt tgtattgaaa
     1861 tttcttcgat tagagatact tattactgtt caaaagcttc tttgaaacgg tgaatcaatt
     1921 cattgactac aggtgataaa atccgattct ttttccagac tagcatgcag ttcgtttcaa
     1981 tcattgggtc aagcggaacg aatgtcactc gctttgaaag atgacttaca gcaccttcta
     2041 tgactaaagc agcacctacc tgattctcaa ttagcgcaaa tacattgaaa atcaaattgt
     2101 acgttcctgt gatcagcaac tgctcctttt ttatgcctaa ccagtcagca aacattcgct
     2161 gtatttctgg acgctttgag atcaaaagag gtacattcgc aaagtcttct tttgttatca
     2221 cgtctttttt tgctaaaaca gaattttcta aaatgagcaa tccccatttt tcctttcttg
     2281 gtaacaaaat cgtttcgtat ttctctgtat ctactggatc tagcaaaatc gctaaatcta
     2341 acaggctttt ttctagtcac tccgtgatat catcacttgt tccgctgtgg atatgaaatt
     2401 gaacgctggg ataatcacgg atcaactttt ctagtaatag tgccaatgta ttcgaattat
     2461 cagcttccac acagccgatc gtaaatcggc cgctgaataa ttgttttttt ctgttctcga
     2521 attcttgagt ggtttgattc gttaaatcta aaatttcttc tgctcggctt ttttagaaaa
     2581 taacctgctt ctgtcaagat cattttgcgg ttttcgcgta caaacagttc tgttcctagt
     2641 tcctcttcta actccttcag ttgtcggctt aaggttggtt gtgtaagatg tagaacttct
     2701 gcggcttgag agattgttcc ttcttctgca attgtccaaa aatattttag taagcgtaac
     2761 tccatagcga tgctccttga tttttttact tgatacattc cattttattt ctagataaca
     2821 cacgatcata tttcttgatt tttccttgta aacgttccaa acactcttgc atttcagcaa
     2881 tttctttcgt caaaagttca ctttcttcaa tcaaaagctt acgccgctca aatacagttg
     2941 cttctccttc gctagatagg cgaatatatt caatcaaaga ctcgatcgat aatccggcat
     3001 ttcgcataca gatcgtaaag tcgacccatt ttaggtcttc ttctgtataa tcacggattc
     3061 cgccttttgt ccgattaact ggtggaatca acccgattcg ttcataatag cgcaatgtat
     3121 cggccgaaat gcccttttct tcactgactt gtttgatatt catctgatca ctctttctct
     3181 taatttcttg ccactttact tatgtatttt atttattcta caactttaag tgtactccaa
     3241 gtcaatactc atttctatta aaactaagct ggattatcta tatcggttgt ttgtgtatta
     3301 tttcaaataa aaaagtcttg tttttttatt cttttttgga tatactttta acaatttagg
     3361 gaaattttag gaaaaggggt ataaaaatga caacgtatca gcgaaaggta ttattatcga
     3421 catcggcggg aattgcttta gagaatatgg atattatgtt tttagctttt tcattatctt
     3481 caatgattac ttcttttcat attagtggta cgcaagcggg tttcatttca acgatcacta
     3541 atttggggat gctcattgga ggtatttttt ttggcatcat agctgataaa tatggacgtg
     3601 tgaaagtatt ttctcaaacg gtccttctgt tttcgattgc ttctttattg atgtattttg
     3661 cttcaaacat ctacttggtt tatctctttc gctttatcgc aggtgtagga gcgggaggag
     3721 aatacggggc gtgtatgtcc ttgatttccg aaagtttttc aaaaaaacaa attggacgag
     3781 cttcttcggt tgctggtatt ggtgcgcagg tcggtgcggc tttggctgcg attttagctg
     3841 ctgtgatcat tccaacgttt ggttggaaga tgttgtatgt cgtaggtgta ctgccggtgt
     3901 tgatggtctt gttcattcgt cgcggcttga ctgaaccaaa ggaattcaaa tctgtacaat
     3961 ctgccgaaaa gaaaaccaaa cttagcagac tgtttgaatc aaaacatctt gcttggcaaa
     4021 catgtggcct tagtatcatg gtaactgtcc aaattgccgg ctattttggg ttaatgaatt
     4081 ggctgccttc aattatgcaa aatcgattag ggctttcggt ttctgggtct tcattgtgga
     4141 tggtcagtac tattttaggc atgtctttgg ggatgatgac ttttggcata atcatggata
     4201 aattggggcc taaactagcc ttttctttgt ttttaatttg ttcatcctta tctgtctatc
     4261 tacttgtgtt agcccatagc cagtggagtt tggtagcagc cgctgtgata gtcggctact
     4321 ttatcaatgg catgtatggt ggatatggcg cagtgattag tagtttgtat ccaacagaaa
     4381 tccgcgcgac ggcgaataat ttcattatga accttggacg agctgttggt ggtttttctt
     4441 ctatcgtcat cggtttcttg atggatcatt atagtttaac tgccgtaatc ttgtttttga
     4501 gcggtattta cctagtcagt ctgctggtct tgacaactct tactggtgtt acagcattaa
     4561 gggaaagctt gcagcattga tagttgagtc tgtaaatgac aagaaagagg tgtggcaaaa
     4621 gcttgcatct gcggtaataa gaccagccaa atcaaaaaca tgagacactg tttccgtttg
     4681 gctggtcgta ttacttgatg cagggcagct ttttcaactg ctaatttcct tctatctggc
     4741 ggcgactatt ctttgacgtc cgtcatttca aagtacttta ctaataactt agcataacga
     4801 aaaactgtgg atacactcgt atattgagcg tctgcataat cttccaaatt tttctcagga
     4861 tgttgataca tgaattcaat caaatgataa ttatatgatt ttctgtagaa atgaagataa
     4921 ataatgtaga ttgggaagtt ctcctgcttt tccatagaaa agcgccgatt attctcattc
     4981 ttcaaaatga ccttatcagg atatttttgg aacaattctt ctaaatctgt tagataattc
     5041 aagatagact tacttgatac cttcaattcg ttttgtagtt cttctattga acaccaacgt
     5101 tctgtttcaa gcagtaaatg aatcatttta attttctttt gagaaacttt tgttaaaaaa
     5161 gtctgcatat tttctcctcc tgtatctact ttactatact acatacaaga aaaaaaacgg
     5221 atacaaatat catattgtca c
//
LOCUS       DACCXM010000148         5210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCXM010000148 DACCXM010000000
VERSION     DACCXM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.156"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            32..388
                     /locus_tag="IVY61_002537"
     CDS             32..388
                     /locus_tag="IVY61_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971600.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="IVY61_002538"
     CDS             404..754
                     /locus_tag="IVY61_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP7971601.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="IVY61_002539"
     CDS             783..1562
                     /locus_tag="IVY61_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP7971602.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="IVY61_002540"
     CDS             1573..2235
                     /locus_tag="IVY61_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP7971603.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="IVY61_002541"
     CDS             2254..3360
                     /locus_tag="IVY61_002541"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP7971604.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="IVY61_002542"
     CDS             3350..4441
                     /locus_tag="IVY61_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP7971605.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="IVY61_002543"
     CDS             4454..5194
                     /locus_tag="IVY61_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP7971606.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1666 a          860 c         1247 g         1437 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag
//
LOCUS       DACCXM010000149         5166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCXM010000149 DACCXM010000000
VERSION     DACCXM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.157"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(599..889)
                     /locus_tag="IVY61_002544"
     CDS             complement(599..889)
                     /locus_tag="IVY61_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971607.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1231..1527
                     /locus_tag="IVY61_002545"
     CDS             1231..1527
                     /locus_tag="IVY61_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971608.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1534..3486
                     /locus_tag="IVY61_002546"
     CDS             1534..3486
                     /locus_tag="IVY61_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7971609.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3558..4055)
                     /gene="dfrG"
                     /locus_tag="IVY61_002547"
     CDS             complement(3558..4055)
                     /gene="dfrG"
                     /locus_tag="IVY61_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7971610.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4382..5074)
                     /locus_tag="IVY61_002548"
     CDS             complement(4382..5074)
                     /locus_tag="IVY61_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7971611.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1669 a          900 c          860 g         1737 t
ORIGIN      
        1 aaaagaaatc tacgatagtc aacaaaaaaa ttttcattat atccagaaaa atttttctta
       61 gttaaggacc tatggattgc agtaatgcag aattcgtcga ataaactgcc aaagacatac
      121 aatcatattg cttttaaaat tgacgaacaa gatatagatt cgtttgtctc aaaaatccaa
      181 atgttaggtt taactgtcga accaggacgt tctcgtgtga aaggcgaggc ttcttcaatt
      241 tacttttacg actatgataa tcacctcttc gagttacata ccggtactct ccaggaacgt
      301 ttaaaatcct acaattccac tacctgagac tagtaatgac tagtctcttt ttatggcctc
      361 tgatcccccc attgagcgaa tactaatagg gcttcgaatt gatctgttca atctttaagc
      421 aatatccctc aaatgagttt ggaaagttta gtgattcatc acaaatactt aaatacatgt
      481 agttatatat agattgaggt tcttgtgagc gtatgctcgt gtgggttcaa atcccatcgt
      541 ctgcatccat ctttccaaaa caagcggcta ggacattttt gtctcagccg cttgttttct
      601 agtttattct ttcatcttgt cggacctttt ttcttgggta tagacttttc tcatattcta
      661 ccacaatttt gggtttccct aacacatgat aggatctcac aacaaattgc tttctattat
      721 tataataacc tgtaacgact aacttcatcc cttcgtctac ttcagctagg aagttcaaac
      781 tatgggaagc aatcaaacaa ttgaccccat ttaaactgaa acggatcaac ggtctggcac
      841 taacttgcaa agtacggatc ttatctactg ttccatattt tttctccata accttcacct
      901 caataccatt atacgaacat acattccctt tgtaaagaag aaactctgtt ttatcttttt
      961 gactttttgt ctattacctt tcttaaaatc atattggcac ttcctgcgaa gatgaaggac
     1021 atgtactaaa aaaaagaggc agcagtatag atttaagtag ttcagttttg gagtacaaaa
     1081 agtgaaaatg taaacctaaa tgagtatcaa attgcaactg aatttgatac tcgtttttta
     1141 ttgttaaatc aacgtttgtt gttaatttac accacaaaat gtaagttaaa atgtatataa
     1201 aaatgcgaac gtgaaataat tattgtagga atgcaaattg gtgtgttaaa atggttacaa
     1261 tttactaagc aaaaaggggt gcattttcct atgcaatttc ttgagccaaa aaataagaac
     1321 gctaagtctg tggattggga aatatcagag caagtaaggg tgatagttaa gcaatacgct
     1381 gaatatgcag agcgtactga aagcgaagca gtggatgaat tcttattgaa cattttagac
     1441 gataagaagt ttattgaatg gattgccaac aagcgaagta ataagcggat tgttgaaaaa
     1501 atgggtatca aagatagagt gggttgatag acaatgacca agcttaaaag attagccacg
     1561 aaggaagatg aaattgtaga tgttaaaatt ccgatttcaa acgaagaatt aaaagatagg
     1621 gctaaacaat atgatttatt aactccaaaa cgatttgcaa caaggtataa caaaatgttg
     1681 ttcttgccaa cgtcttttaa atggaacggt tctgaatatc ccattcaata taattactgc
     1741 atcaatccgt tttgttgtaa ttttgggaaa gaacagcaca aattcaaaga tgtaaaaggg
     1801 aaaccaagtc gatataaaat gactggtagt tccaaagata aaggtcataa aggtatgtac
     1861 tgcaatgaca atcctattgg cagaggtgta tcgcaaaact gtacagtaac ccctttatct
     1921 aattggtctg ttgttgaaga aattaaacgc cttatagaaa tcaatagcat ccaagatgtt
     1981 gagccagatt atcaatttca taaagaaggc tgctcggaag aagaatcaac cccattcaat
     2041 gagccgaagc aattttacaa aagggggaaa agtaggggca aatctcaacg ctatcaatgt
     2101 aaggcgtgta agaagttcac taatgttcta ccaaaaagag aagaaaccac tacttatcac
     2161 caacaaaaga acaccattct tccaatgttc gctaagatgg ttgttggaag ggtttcagtc
     2221 agtcgtactt gtgatatttt aggaattgga gttggaacct actatcataa gctggagtgg
     2281 ctgtataggc gttgtttgga gtttttagaa cgatatgaga cacaacccct tcaaacaaag
     2341 aaatttaacg agatgtggct taatacggac aagatgcact actacctaaa taacgtccgt
     2401 aagaaaggac aaggttcaaa gaaatatacg gggtttgaag accttaatat gcaaacgtat
     2461 atagttgtat ctgctgaggt cttatcaaga tatgtgttcc gttctgatgt tgcctatgat
     2521 tggaatattt caatggatga attaaatgaa gatacaagaa aattcaaaga agaccatctc
     2581 aatactttta gtcgtaaaaa tgataggtta gattggtctt attatccaca agaaccatcc
     2641 gctaacgata gcgaaaatag gaatgcttat cttcacgaat tagggaaaat tactaatagg
     2701 agtagatttg tcgatggttt aaatgttgat gctccttata ctacaactgc tcactattgg
     2761 ttaattaaac aaatggttaa tgccgacgaa tggcgaatga ttagtgatga tgacttctct
     2821 attagaaacg ccttttatag agtattcacg aaagaattaa ggttgagcga tgcacatcat
     2881 tttatttgcc aagttaacaa gacgaaaagc agaaaacaat gcttaaaaga attcggacaa
     2941 gcaaaagcag aactgctcga ttggggagat atcagaggat tcaaaacaaa gtttctacgg
     3001 actttggcat ctcattatct aactgaatta ctaacaagtc atcaattcca cgaagaggct
     3061 attagtaaag atggagaaag atatcgcaaa tatgccgata accctatcaa acatccatta
     3121 gccactaagg ataaaggatt ttattcagtt gattgtagaa cggatttatc tgctctggaa
     3181 ccaaacgaaa tcgcaaagat gctattaaat gttaatgacc attcgaccaa tagctttatt
     3241 caacagataa gacgttatat atcatctctt gaaagaccat taacaacagc tcgtggtgat
     3301 aagaagagct atatttacgc taacttcaat ccgaagtatg ctcaatttgc gattacaatt
     3361 ctaagaactt actacaactt ttgtcgccct tttaaatcag cggataaaaa ggttttgaca
     3421 cctgctcaaa gattagggat tacagataaa caatttgatt ggaaagatat aatttatttc
     3481 aaatgacaaa aagagcttgg agtaatccca agctcttgtg ctttatcttt actgttcttc
     3541 ttcaattaac gcacccgtta actcaataag ttttttcttt catatacatg aaaatagtag
     3601 ttatacggat ttttatcatt ctttatccct ttttgggcaa atacctcatt ccattcctca
     3661 taattcactt ctggaaaaaa agtatctcct tcgaattcat gatgtatttt tgtgatgtac
     3721 attttctcaa cataagggaa aaacaaatta taaatctgtt ctcctccgaa aataaaaatt
     3781 tcttcttcgt ttttacataa ctcaaaaaca tcttctattg aatgaacaat ttcacaacca
     3841 ttaaaggtaa accccttatc tctcgtcaga ataatatttc ttctgtcagg taaggctctt
     3901 ccgattgatt caaggttctt cctacctaat attatcggat gtccctttgt agtattttta
     3961 acatattccc agtccttggg aatcctccaa ggaatgtcat tctctttgcc aatcactcta
     4021 ttcttatcca tcgcagcaat caaagaaact ttcatttata acacagcctt tttaactaat
     4081 ttaccatcta atttcttatt tctatcaaaa aaccttttct cattatcctg cccgttagtt
     4141 gaataagcaa ttttttctat aagttatatt atcataatac cacaaaatca caaattttca
     4201 ttcaattatc attttcaatt gcattttata aatcattttg tatgtaattt ggtatgtaag
     4261 ttaaaatgaa aaaagtagtc cattttgaag tacattttgt cattttgtac tccaaaactg
     4321 aactacttag tatagattac tgttgcttct ttactaattt aggtttactt gttaagaaag
     4381 attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta tcttccactt
     4441 catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt tcagcagttg
     4501 cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt ttttcttgtg
     4561 tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg
     4621 cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt
     4681 tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt agttgtgcat
     4741 ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt
     4801 tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg
     4861 tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct ttatctgtca
     4921 atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc
     4981 acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa
     5041 cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa tccaatactt
     5101 ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa ttacatatgt
     5161 aatttt
//
LOCUS       DACCXM010000150         5110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCXM010000150 DACCXM010000000
VERSION     DACCXM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.158"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            35..1969
                     /locus_tag="IVY61_002549"
     CDS             35..1969
                     /locus_tag="IVY61_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7971612.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2426..2842
                     /locus_tag="IVY61_002550"
     CDS             2426..2842
                     /locus_tag="IVY61_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP7971613.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2856..3422
                     /locus_tag="IVY61_002551"
     CDS             2856..3422
                     /locus_tag="IVY61_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP7971614.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3445..4431
                     /locus_tag="IVY61_002552"
     CDS             3445..4431
                     /locus_tag="IVY61_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7971615.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4586..>5110)
                     /gene="scm"
                     /locus_tag="IVY61_002553"
     CDS             complement(4586..>5110)
                     /gene="scm"
                     /locus_tag="IVY61_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7971616.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         1537 a          945 c         1134 g         1494 t
ORIGIN      
        1 attatataga tagtctataa ggagcggatg atcaatggca aagcaaaata ttgtcgttgt
       61 aggagcagga tatgccggtg tttcagctac aaaatacttg gcaaagaaac tgaaaaaaga
      121 agatgtagca attactctga tcgatcgtca ttcgtaccat actatgatga ccgagttgca
      181 tgaagtagct ggcggacgtg tagaaccaga agcaatccaa tacgatctgc agcatttgtt
      241 tgctcgtcaa aagaatgtac aacttgtcac agacactgta atcggtatcg ataaagaacg
      301 aaaaatcgtc aagaccaaat taggttccta tccttttgat cagctgatcc ttggaatggg
      361 cggagagccg aatgactttg gtacacctgg tgtcaaagca cacggattca ccctttggtc
      421 atttgaagat tctgtaagga ttcgggaaca tattgagaag acagtagcaa aagctgcatt
      481 ggaaccagat gatgaactgc gtaaagccat gctgactttc gttgtctgtg gttctggatt
      541 taccggtatt gaaatgattg gggaactgat cgactggaaa aatcgcctag ccaaagatta
      601 taaattagat ccaaacgaaa tcacactgat ggtcgtagag gcaatgccga cgatcttgaa
      661 tatgttggat cgaaatgatg ctgcaaaagc tgaacgctat ttgcgtaagc acaacgtgga
      721 attattattg aatgcaccaa tcgtagaagt ggctgctgat catatcaaat tgaaagacgg
      781 acgcaacatt ccgactcaca cactgatttg gacagctggt gtcaaaggaa cttctgacgc
      841 tgctgacttt ggattagaat cagcacgcgg acagcggttg atcgccaatg aatatatgca
      901 ggcaaaaggc tacgaagata aaaacatcta tatcattggt gatttagtct tttatgaaga
      961 attcccagga acaccgacac cgcagatcgt tcaagctgct gaacaaacag gacacacagc
     1021 cgcagcgaat atcgtggctt cgatcaaagg cggagaaaag cataaattta aaggaaacta
     1081 tcaaggattt atggtttcta ttggtgccaa atggggggta gctaacctat ttgataagat
     1141 ccatttaagc ggatttttag ctatcgtaat gaaacatatc gtgaatttga aatacttctt
     1201 tgatattcgt tctggttatt atatgttcca atatttaatg catgagtttt tccatatcaa
     1261 agatgatcgc aacgtgactc gaggacattc ttctcgttac ggcaatgtct tatggagtgt
     1321 gccacttcgt attttctatg gtttagtttg gttcgttgaa gcttccaaaa aaatcattgg
     1381 gaatggtgaa gtgttcaaac caagtacttg gtttggtgaa ggatcatggt tcactggaaa
     1441 cagtgtgttc ccttggacat gggaatacaa tgtaacagat gttgccagtg gtgcttctgc
     1501 tgctggtgat gcaaccacag ctgccagcgg agcaggggca agtgccggag aagcagcgac
     1561 tcaagctgca cattttggtt tgagttatgc atacggtgaa gaaccaatgg ctgtgattga
     1621 gaagtcacca gactggttca caaagatcat gcaagtgatc atgccaaaca atgatgtagc
     1681 aatgttcttc caaaaattca tggtattctt tgaaattgcc ttggcattag cattgattgc
     1741 tggattattc acatggctat gcagtgcgac aacgattgta ttggtgatca cgttctgctt
     1801 atcaggcatg ttttactggg taaacatttg gttcattttt gttgctttcg cattgatgaa
     1861 cggttccgga cgtgcgattg ggttggatcg ctgggtaatt ccatggctac aacgtacact
     1921 aggtaattgg tggtacggta gaccaagttc ccagtacggc aagaaataaa tagataaaga
     1981 acgttatttg aatgcaagat ttcaatagaa aggggctgag acaaaccgtc acagcctcct
     2041 ttttatgtaa aattaaagga ataagatgcc cgagataagc ctccatgagc aagacacagg
     2101 tgtcacaacc tctgttacaa cctcttgtta taaacggtgt tccagaagta gcttcttcga
     2161 aaaataagcc gaaatttcac aaaaacttga aaagtaattt tcgtaaattc cttcttattt
     2221 cttgaagttg cccacttctg tcacaacctc ttgttataaa cggtgttcca cctgctgctt
     2281 ctcggtaata agcccaaaga attagaaaac atggaaagct gttttctatt cttcgttctt
     2341 attactcaaa gcaagacaca ggtgtcacaa cctcttgttt ttatgctaaa attgggtgag
     2401 ttaatctgga aaaaggagaa caaaaatgag ccttaaagaa tttgctgaaa aaagtctgct
     2461 caaaccttgg gatgttttta ttattttgct tttgactgtt ctttcttttt taccagtcat
     2521 gatttttggt cttcagcaaa acaccgcaac agcagaaaaa gaagcggtac tgcgtgtgga
     2581 tggcacagaa atcaaaacat tccctttgat ggctggagaa gagagcaaca catatactta
     2641 caaagatgaa cacggagatt ataacttgat cgagatagat ggcgatcgag tacggataaa
     2701 agaagcagat tgcgatgacc agatctgcgt acgtcgaggc tgggcaacga aaaatggcga
     2761 aaccatcgtc tgtttgccac ataaattagt gatcgaagtc cgttcaacag atggaggtga
     2821 cgaagacagt ctgatttatt agaccttcac acaccatgag taaattacaa aaaatgattt
     2881 atatttcgct acttgttgca caaggagtga tcattggttt attggaaaat atgatccctt
     2941 atcctttttc ttttgcgcct ggtgcaaaac taggcttagc caatctaatc acgatcgttg
     3001 ccatttttac catgaaaaaa cgagacagct tcttactact ttggctacga ctattcctga
     3061 ctactttgct aggcggaacg atttccacat tcctttatag tatgagtggt gctttgctta
     3121 gttattttgg aatgttattg gtcaagcaat taggaccaaa acgtgtcagt atcatcggaa
     3181 ttagtgcggc cggcggtttc atgcataatg tcggacagct tttgatggct tctttcattg
     3241 cacaatcatg gacagtcatg ttgtacttgc cagtcctttc agttttaggg attttatcag
     3301 ggattgcaat cgggatcgca gcgaattatc ttttacgaca tgtgcagaca cttcaacgat
     3361 tccaaaaaga ttatgagcag ggaaccaaac aaaaatggag cccaatgttt accaaaaaat
     3421 aatcgtcaca caaggagttt ttttatgaag ctacatccaa tgtgggaaaa ctatcctgct
     3481 ttaagtaaag aattgacaga aacactaaaa cggatggaag aaactgtccg cctgaaaaac
     3541 aaacctgtcg aacaagcaat caaagaaacg atccatgccg gcggaaagct gttgcgtcct
     3601 gcctatcagc tattgttctc acaatttgga ccagagcaag atcgagacaa agccatcgct
     3661 ttggcagctt ctatcgaaat gctccatacg gccacactga tccatgatga tatcgttgat
     3721 gaagcggatc tgcgacgtgg tcagcctaat atccgtagtc agtttggtaa tgtcgtttct
     3781 gtctatgcag gagactattt atttgtctgc tgttttaaac tattgtctga ttactccact
     3841 tccttgaaaa gtctccaatt gaattctcgc agtatggaaa aagtattgaa tggcgaatta
     3901 gggcagatgg atgatcgcta taattatgaa ctttctgtaa aagaatattt ggaaaatatt
     3961 tcaggtaaga cagctgaact gttccaatta agttgttctg ttggcgcttt tgaaagtgga
     4021 acaagcgagc gcttcgcaaa aaaagcagga gatatcggac tttctatcgg gatggccttt
     4081 cagattatcg atgatatttt agattataca aaagagagcc aggaaatcgg caaacctgta
     4141 ctagaagaca tgcgtcaagg ggtctattct ttaccgttga tctactcttt gcaaaaaaac
     4201 aaaccaaagc tactacctta tttagagaag aaagcggcaa tgacagaaga agatgtcgac
     4261 gccgtacgca aaatcgttga acatacagga ggcgtggaag aagctaggaa gttagcggcc
     4321 tcttacacag aaaaggcttt gaaggaaata aagaaattac ctgccacctc tcttcgaaca
     4381 aaagagaatc tcttttccct tacccaactg attttggatc gcaaagactg aatagggaaa
     4441 ggttcgatac gtaaataaat ttctcagaag gaaatctacg tgttttttga caaattgaat
     4501 tgtagttctt gtatttatga taaaaagcaa aaataaagag caacgtccgt tagcgacgtt
     4561 gctctttttt atcttgaata tttttttacc ttttcgattt cttataactc ataaaagtga
     4621 ttcttaaacc gattacaagt aatataacgc ctgcgagtgt tatccaatta ttcgattgac
     4681 ttccagtttt aggaagtgct ctttttgatt tgtttccatt gtcattgctt gttttattag
     4741 ttaagttaaa gttcgtcctg ttatccgaac ttgtttttga gaaggagaca cttgcatgac
     4801 gtgtatcgct tgtggtagac gacgtgtctg tactatcgct tgaagttgat gaatctgagc
     4861 tatcacttgt ggtagaggat gtatctgtac tgtcacttga agctgacgtg tccgtgctat
     4921 cacttgtagt ggtagatgaa tctgagctat cacttgtggt agaggtagta tctgtactgt
     4981 cacttgaagc tgacgtgtcc gtgctatcac ttgttgtggt agatgtatcc gtactgtcgc
     5041 ttgaagctga cgtgtccgtg ctatcacttg tggtagaggt agtatctgta ctgtcacttg
     5101 aagctgatgt
//
LOCUS       DACCXM010000151         5080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCXM010000151 DACCXM010000000
VERSION     DACCXM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.159"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..253
                     /locus_tag="IVY61_002554"
     CDS             <1..253
                     /locus_tag="IVY61_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7971617.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(396..1544)
                     /gene="nagA"
                     /locus_tag="IVY61_002555"
     CDS             complement(396..1544)
                     /gene="nagA"
                     /locus_tag="IVY61_002555"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP7971618.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1620..2456)
                     /locus_tag="IVY61_002556"
     CDS             complement(1620..2456)
                     /locus_tag="IVY61_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971619.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2640..3857)
                     /locus_tag="IVY61_002557"
     CDS             complement(2640..3857)
                     /locus_tag="IVY61_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7971620.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            4240..4323
                     /locus_tag="IVY61_002558"
     tRNA            4240..4323
                     /locus_tag="IVY61_002558"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4274..4276,aa:Leu,seq:gag)
     gene            complement(4563..>5080)
                     /locus_tag="IVY61_002559"
     CDS             complement(4563..>5080)
                     /locus_tag="IVY61_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971621.1"
                     /translation="NYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSN
                     LNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGF
                     CSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLG
                     ICTFIITVINR"
BASE COUNT         1537 a         1072 c          828 g         1643 t
ORIGIN      
        1 tacggaaggc tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa
       61 aaagaaattc acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgcc
      121 ccactcaaac ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc
      181 gtttggttat cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat
      241 ctttgaaaac taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
      301 ttcaaatttg tcttcgccaa cacaatttga cgaagagcca aaaaacctta aaaccaaact
      361 taagaagttt agttttaagg ttaacttaaa ttatattatg cgtggaaacg ttccacgcca
      421 tctagataag ttgcttccaa tgtcatatca gggttcaata cgataaagtc tgcatcgcgt
      481 ccttgagcga tttttccaca tgtatcatcg attttacagc tgattgctgg aatcagacta
      541 gccatcatga ctgcttgttc tggtgttgct aatccccagt ctacaacatt tttaattgct
      601 tctttcaatt tcaatatact tccagctaag ttgccagatt ctagacgtgc tgttccttct
      661 tttactacta ctggaaattc gcctaaattg taatttccat caggcatccc accggccatc
      721 atacagtctg taattaatgc gacatgatca tgtcctgctt tttccattag gattcccgca
      781 gcatttggat gtacatgatg gccatcacaa atcaattcag aaaagacatg ttctaaagat
      841 aataacgcac ccaccatacc tggttcgcga tgattcaatc cacgcatacc attgaaagca
      901 tggacaaata cacttgcgcc tgcttcaaca gctttttgcg cttcttctag tgtagcatta
      961 ctgtgtccta gagaaacaac tactccttct tcagtcactt gtttgacaaa ctcttctaca
     1021 ccttgacgtt ccggtgctaa agcaattttt ttgattaaac cacctgaagc ttcttgccat
     1081 tcatggaaag tatccaaatc aggatccccg aaataacttg ggttttgtgc ccctttgtgt
     1141 tcttctgtga agaaaggacc ttcaaaatag attcctttga ttttagctcc tggtgcttcc
     1201 tgatacattt tgccgattgt ctcagctaca tcttttaaac gttctttgct tgacgtcaat
     1261 gttgttggta aaaatgaagt cactccacaa gataacagac cttcagacat cactttgatg
     1321 ccttctgcat cattatccat tacatcatga ttcatatagc cgtgaatatg tgtatctacc
     1381 aaaccaggag caatccattt gccgctttgg tcaatgatgt tgacttctcc ctcaggtagt
     1441 tctttggtgt attcaccaaa aagtccatcg gtaatttcaa gatagcctgc atgtttgaca
     1501 cctgacgcta aaaagaattt gtcagcatag ataaaagttc tcattctatt ctctcccttt
     1561 gtttttctac ctctaaagta ctcttattta gagaagaagt caagaatggt ctacaccatt
     1621 tacaaatttt ttgaaatttg tttcacagct tgtgctaagc cttcatagaa tgctgctctt
     1681 tctggttctt ctttacgctt gtatattaag cttttaagca caaataaatc attgaggtaa
     1741 tagtagctat tcttctcttg agcccaatag attccttgat tcacctgttc taacgcttcg
     1801 tcgatttcat tttgatggaa taagaaattt ccataattat aaaaaatttg cgaaaattca
     1861 ctttttgatc gttcatcagc tgattgatgc agcagttgga tgcttcgact caaatagtag
     1921 cgtgcttcag ccgtttttcc tgccacacca tatatttttc cgatacaact aatcagttgt
     1981 atttctgtgt cagaaacatg gattctttct ttttggctag agtagcttaa tgcttctttc
     2041 aaataataaa gggactgaga gatattttga cgcaacgtga attcgcaaac accgagataa
     2101 taataatagc gttgaaagtc agttgcttga tacagatttt cagaaatatc agaagatttt
     2161 agtacttgtt ctaacttccg ataatcacgt ttttggaaac aactatccaa cagttcaaat
     2221 aatttatcgt ttgtccgagt agtttttcga gagagattca tcacatgatc aatcgttacc
     2281 cctagccgtt gacaaatctg catcatcacc aatacattcg gcagttcctc attattttcg
     2341 atccgactta atacactttg tgaacaaata tcatgcgaaa gcattttttg cgtcattttt
     2401 tgttcttttc gaatctcttt aatgatcgtt ccaaaggact cgattaacgt ttccataagt
     2461 atcctcccca aaatatgcgt attcgcattc tattcgtatt ttactttagg aagtcgcaaa
     2521 cttcaagaaa agacttgcaa ctaaataata tactttagtt atttaagaaa actccactag
     2581 aaagagagcc tcagtgtgtt tgtctgtggc cctcctactg aaaaatttgg tttttaaaat
     2641 catttttggc gataggttgc taggaattct ctcattttct tagctgtttg tttcaaatca
     2701 tccattttag gaagataaac gattcgaaaa tgatctggct ggttccaatt aaatccacca
     2761 ccatgtacaa gtagaatatg atgttcatgt aaaaagtcca aaacgaattt ttcatcatcc
     2821 aaaatattga atcgctttgt atcaattttt gggaatatat aaaaagcagc ttttggtttt
     2881 acggctgaaa gaccaggaat atcattgatt gcattataga tatattctct ttgttcatag
     2941 atccttccgc ctggcaggag cagctcatcg acactttgat aaccgcctag cgcggtttgg
     3001 ataatttgct gagacaatac attggaacac aaacgcatgg aggacaacat attcagccct
     3061 tctatatagc ctttgacgtg tgacttatct ccacttagga ccatccagcc tactcgaaat
     3121 cctgctacac gatgagattt ggatagaccg ttcagcgtaa caacgaacaa atcaggcgct
     3181 aatgtagcaa tcggaatatg ttctagacca tccatcacta agcgatcata gatttcgtcg
     3241 gaataaataa tcaggttatt ttgtcgagct acttcaacga tttcttctaa taattctttt
     3301 ggataaagtg ccccagtagg attattagga ttaatgataa caatagcttt tgtccgactc
     3361 gttatttttg atttgatatc atcaatatca ggataccatt ctgcctcttc atcgcaaata
     3421 taatgaacag gtgtgcctcc agctaatgca atagaagccg tccacaacgg atagtccggc
     3481 attggaacca gtacttcatc tccattgtcc agcaacccct gcatacacat cgtgatcaat
     3541 tcgctcactc cgttgccggt gtagatatca ttgatcgtca cattcggaaa atgtttcagt
     3601 tggcaatatt gctcaattgc ttttctagca gaaaagatcc ccttggaatc tgagtagcct
     3661 tctgagtcac gggcattcat gatcaaatca cgaatgactt cgtttggtgc ttcaaatcca
     3721 aaaggtgcgg gatttcccgt gttcaatttt aaaatactaa tgccctcttc ttgcatccgc
     3781 tccgcttctt ctagaacggg accgcgaaca tcgtaactga caccttctaa tttgttggat
     3841 ttttcaaatt ctctcatcac ttagccaatc cctcatttca aaaagttaga actttccaaa
     3901 ttttttgtta tttactattt caatatcata ctccatccta gaaaattttg ccacttattt
     3961 aggaaaaacg taagtattaa aaaaacacaa acaagcgttg agaacttaat tgtcatgata
     4021 gtcattccat agattcgctg cccaatccat tgcaattcca caaatgattc ctagcataaa
     4081 accaagcaaa aaacgtccga atgcaagtcc aatcaataca cctaagatca ttcccagtcc
     4141 tgaaaattct ccgtcatatt tctccatctt aataagtcct cccatttttg agcttgacaa
     4201 ctatgtaaaa cctcgctatc attagtgtgt tattattacg cagatgtgtt ggaattggca
     4261 gacaagcatg attgagggtc atgtgagcgt tagctcgtgt gggttcgagt cccatcatct
     4321 gcactacaac tcgtgaagtt aattttgctt cacgagtttt tttgtttgaa cgtttcttta
     4381 tgccattata tacatatcac tgtatccttt acaaaaagaa cgtacaatca tgtaaacagc
     4441 ctgaggtgag tatatgaagg tcggaaacaa gctactcgta gaaacttgca agtgttaaaa
     4501 caattaatcg aaaaacgagt aaaatccctc acctaatact aaagcgaggg attttttgta
     4561 tattatctgt ttatcaccgt tattataaag gtacaaatac caagcacggt taatccaaat
     4621 tgcataaatt catttttttg tacactcatg cttttcatta gctctttaac tgccattagt
     4681 actaagatgg cgataacaac ataaagtata tagtacgtcc gcattccgga agcccatact
     4741 gtgggagaaa atcccataat taaccttgaa caaaatccca ttatcatcaa aataactaaa
     4801 aaaatagaag ttttcttatt gtcaaaactt aaataaattc caacaattaa acatagtaat
     4861 aatgccaaga ctactaaagt agctatccaa gtacctggat aaaaaagtga cagtttcgtt
     4921 cctaactctg taaagagatt attcaggtta ctactactcc aaatcattgc ccttttattt
     4981 ccattgacgt aagtaaagct ttgacccatt gtattaccaa aataaataat caggtttaag
     5041 aaaaacggta atgcagtcaa tatacgcaca taatagtttt
//
LOCUS       DACCXM010000152         5025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCXM010000152 DACCXM010000000
VERSION     DACCXM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.160"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            283..522
                     /locus_tag="IVY61_002560"
     CDS             283..522
                     /locus_tag="IVY61_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971622.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="IVY61_002561"
     CDS             complement(884..1144)
                     /locus_tag="IVY61_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971623.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="IVY61_002562"
     CDS             complement(1329..1826)
                     /locus_tag="IVY61_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP7971624.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="IVY61_002563"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="IVY61_002563"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP7971625.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="IVY61_002564"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="IVY61_002564"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP7971626.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..>5025)
                     /locus_tag="IVY61_002565"
     CDS             complement(4548..>5025)
                     /locus_tag="IVY61_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7971627.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1529 a         1043 c          771 g         1682 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaa
//
LOCUS       DACCXM010000153         4933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCXM010000153 DACCXM010000000
VERSION     DACCXM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.161"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            85..972
                     /locus_tag="IVY61_002566"
     CDS             85..972
                     /locus_tag="IVY61_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7971628.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IVY61_002567"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IVY61_002567"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP7971629.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IVY61_002568"
     CDS             2274..2720
                     /locus_tag="IVY61_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7971630.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="IVY61_002569"
     CDS             2993..4345
                     /locus_tag="IVY61_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP7971631.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="IVY61_002570"
     CDS             4516..4803
                     /locus_tag="IVY61_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971632.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DACCXM010000154         4902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCXM010000154 DACCXM010000000
VERSION     DACCXM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.162"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(406..780)
                     /locus_tag="IVY61_002571"
     CDS             complement(406..780)
                     /locus_tag="IVY61_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7971633.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="IVY61_002572"
     CDS             complement(803..2203)
                     /locus_tag="IVY61_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP7971634.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="IVY61_002573"
     CDS             complement(2207..2617)
                     /locus_tag="IVY61_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7971635.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="IVY61_002574"
     CDS             complement(2635..3126)
                     /locus_tag="IVY61_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7971636.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="IVY61_002575"
     CDS             complement(3136..3927)
                     /locus_tag="IVY61_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7971637.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="IVY61_002576"
     CDS             complement(3920..4693)
                     /locus_tag="IVY61_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7971638.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1556 a         1001 c          650 g         1695 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg gg
//
LOCUS       DACCXM010000155         4739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCXM010000155 DACCXM010000000
VERSION     DACCXM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.164"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IVY61_002577"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IVY61_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP7971639.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="IVY61_002578"
     CDS             complement(2066..3064)
                     /locus_tag="IVY61_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP7971640.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="IVY61_002579"
     CDS             complement(3290..3943)
                     /locus_tag="IVY61_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP7971641.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IVY61_002580"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IVY61_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP7971642.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1390 a          998 c          742 g         1609 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccat
//
LOCUS       DACCXM010000156         4736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCXM010000156 DACCXM010000000
VERSION     DACCXM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.165"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..241)
                     /locus_tag="IVY61_002581"
     CDS             complement(<1..241)
                     /locus_tag="IVY61_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7971643.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYI"
     gene            513..857
                     /locus_tag="IVY61_002582"
     CDS             513..857
                     /locus_tag="IVY61_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP7971644.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            858..1277
                     /locus_tag="IVY61_002583"
     CDS             858..1277
                     /locus_tag="IVY61_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP7971645.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1369..1719)
                     /locus_tag="IVY61_002584"
     CDS             complement(1369..1719)
                     /locus_tag="IVY61_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971646.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1685..3016)
                     /locus_tag="IVY61_002585"
     CDS             complement(1685..3016)
                     /locus_tag="IVY61_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP7971647.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3272..3838
                     /locus_tag="IVY61_002586"
     CDS             3272..3838
                     /locus_tag="IVY61_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7971648.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3983..4070
                     /locus_tag="IVY61_002587"
     tRNA            3983..4070
                     /locus_tag="IVY61_002587"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4015..4017,aa:Ser,seq:cga)
     gene            complement(4122..>4736)
                     /locus_tag="IVY61_002588"
     CDS             complement(4122..>4736)
                     /locus_tag="IVY61_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7971649.1"
                     /translation="AIRNDDDERYLFFSLLAFTGIRKGEAFALTWSDIDFKSKTLNIN
                     KTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLLRKYYTKNKTIVTIQRENLIFHHD
                     GLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHTHASLLFESGASLKDVQERLGHAD
                     IQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGESKGESKNILG"
BASE COUNT         1661 a          809 c          897 g         1369 t
ORIGIN      
        1 caatgtattc tttttcctca gcaggaaaaa tgatttcggt tctcccacaa cgtcgcttat
       61 tttctttgta ttgttcaaag tattcaagtg ctgttccacc acatttgaga aagttataga
      121 cattgtaaat tgtttggcgt ccacgtttaa gctgcttcgc aacgatagca accggagttt
      181 cttgatgaaa atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca
      241 tttatgttca ctctccttgt atgctttagc gggtattaca atttgagtgt aacataaatg
      301 gcttttttat ttgtctcgct taattataca aacggcgtaa aataaaaaaa gcgttagcaa
      361 ctatgcttaa aaagcatatc gactgaataa actaagcatt ggacaggctg aaagactaca
      421 tttaaaatga aatgcgaaga aacaatagaa aataaagttg ccttagaacc atagttgaat
      481 cacctaacag tcgtgaaggg agaataagag aaatggcaga aaaacaaacc gcaggacgag
      541 atcaattagg agattttgca ccaaaatttg cagaattgaa tgatgatgtt ttatttggac
      601 aagtatggtc cagagaagaa ttatctgctc atcagcgtag tatcatcacc atatctgcat
      661 taatttcagg aggaaatttt gaacaattgc cagctcatct gaagattggc aagcaaaatg
      721 gcattactag tgaagaaata gcagaaatca ttacccattt agctttttat gtaggatggc
      781 caaaagcttg gtcagctttt agtttagcaa aagaaatcta tttagaagaa aatagcgaag
      841 ataagaaaga ggcgtaaatg acgacaaaac acgaagaaat acacaatggc gtgatttttc
      901 cagcaggtga aaaaaacgaa gcttacgctc aagtatttac cgggcaaagc tatctgaata
      961 tgttagtgaa tgatcccgaa gtttctgttg gtgtaggtaa tgtaattttt gaaccaggtt
     1021 gtcgcaatca ctggcatgtc cataaagacg gctatcaaat cttacttgtg accggaggag
     1081 aaggctggta tcaggaagaa ggaaagccag caagacgatt aatggctgga gatgtcgtcg
     1141 taacgcacaa agggattaaa cattggcatg gtgcgacaaa agacagttgg tttgaacatt
     1201 tggcgatcac tgctggtaat tcagagtttt tagaacctgt aacagatgaa gtgtatgaag
     1261 gattggaaaa tcaataatgt caagcttgaa cagctcctga aagtagattt taatcgaact
     1321 ttcaggagct gcttttttga ttcagtcttt tataaataga aaatagggtt aataatagga
     1381 atgagaagca cgtcgaaaga tcttaatatt gataatcggc tgggcataca tgatttccac
     1441 gtcatcaatc aaactaccta acagatgata atcttcccga tcatgtcggg gattacctaa
     1501 tagttcttga tagtagattt ccatactagg gtcccgagga ttgttcagca aatcagcaag
     1561 tacatctaaa tcttctggct gagtttctgc cgtaccctgt atctctatga aaagcccttc
     1621 ctcagcaaat tctactccaa tacgtatttc agttatttca tgggaatata aataagtaag
     1681 aagctcatcg atgattcttt ttgataattc tttttccaag tgtctttttt tcctttctga
     1741 cagcggtaaa gttatctagt atcggtacaa ggatacctgc aacgaaacca gttgaaaaac
     1801 cactgttata tagatttagt cctccgtgca aagtgctgac gttatggaca agagcaatat
     1861 gtagtacacc agctattaca ccgtaaaaac tgccgtaaaa accgcttatg ggcgcgagac
     1921 ctgtcccaaa cacagctgca acaagcatgc cagtactggt gatctcatga aaagttccaa
     1981 acaaagaagc aataaatatt ccgactagta tcggaaaact gttttttaaa tgacaaccaa
     2041 aagcactgaa acccaccgca gataaaatag caccgatgac aggtccattt aactgaccgc
     2101 caaccagcaa aacataagaa gacagcatca gaccaactaa gcctatgttg atcaaagtag
     2161 caccaatacc agaattagca atgaaatcag ttgtcaattt tccagatgaa tcgaaaattt
     2221 ctcgaatccc caaaagccga aaggaattat aatagaaacc aattccgact gtcagcgaaa
     2281 aaaagccaaa aataatccaa atgagaggaa agtgatattc attgaaaacc aatgtatttt
     2341 cgacgattga atagctgaat aacctcaaaa cagcagtaaa agtcatcgca atcaacccag
     2401 aggtgaatcc aatattataa agtgtgaatc cacgatggaa aactaaagtt tgagcagcta
     2461 agggcggaag aagtagacca attgctatgc cagctaaatt ccccaataaa atacctatca
     2521 gtaaaggaaa acgcatacca aaagtgatat aactaacgac cggtgataat gcgctaccaa
     2581 acagtccgat catgatgtac tgagaaaaag gtttattgac aaatacagcg tatagataca
     2641 cacctaaaat gattgagata gaattataaa ggtttttgcc aaaaaaagag aaaccggtaa
     2701 ctgttagaat cgaagcaaat aagggaccgt ttaaaatcac cttgtgccga taagctaaaa
     2761 gcacagaaag taaggtcaaa atccctacat ttatgaatgc gcttccgaaa ccgcctaaag
     2821 caaaataatc tgtcaaaaga ttgcttggcg aggtaagaat agttagcaat ccactccaat
     2881 tatcagtagg agtattagaa agcaagccaa taaaaagaaa aaaaacacct gtaaaaccaa
     2941 gataaaaata ctttcttttc tgtgaaacga ttcgtttttg aggatgaata taaataacgt
     3001 cagtttcgtt ttccattaca caccttcttt ttcattttct ttaactataa cttgaaatga
     3061 aagatttgga caactatcat ttaggtcaaa actaaaaatg taagggaacc cattgaattt
     3121 aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag actgaaaaaa aatttaaaat
     3181 ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa taaaacaaca
     3241 tatactgcag taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca
     3301 aattctaaaa gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag
     3361 aaatattgct aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat
     3421 ggaagattta aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt
     3481 ccctattgaa cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc
     3541 aaaaaagcat catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa
     3601 aatgcatgat ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa
     3661 tcaattatcg gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg
     3721 cttagcgtca ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact
     3781 gatcaaacgg acaattgact cgattttagc attagataat cctgaaataa tcagatagtg
     3841 ttctatcagt atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt
     3901 tgctaaaaaa agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa
     3961 aaagtttaaa aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat
     4021 ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatatcaa
     4081 cgattagaca cgatttcgat cgtgtctttt ttgttgtata tttagcctaa aatgtttttt
     4141 gactcccctt ttgactcccc ctgagtactg ttcttcataa atgctacaaa attattgatg
     4201 acttttttgt tttgagtttc agttacatga gtgtaaatat ttgaagtagt ttgaatgtct
     4261 gcatgcccta aacgttcctg aacatctttt aaagaagctc ctgattcaaa aaggagagag
     4321 gcatgagtat gtcgaaaacc atgagtagtg attcttttag tcaattctgg atgatgctta
     4381 tatatgacat taagccacga acgtgaaact gtagggttat agagaagacc atcatggtga
     4441 aaaattaagt tttccctttg gattgtaacg atcgttttgt tttttgtata atactttctc
     4501 aataaactga tcaaatcatt gtccagataa atttttcttt ttccagattt tgatttagga
     4561 gtatttacga tcaatcttcc ttgataacct cttgttacag ttttattgat attgagtgtt
     4621 ttgctcttaa agtcaatatc cgaccaggtc agagcaaaag cttctccttt tcttatacct
     4681 gtaaaagcca atagggaaaa gaataaatat ctttcatcat catcgtttct tattgc
//
LOCUS       DACCXM010000157         4693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCXM010000157 DACCXM010000000
VERSION     DACCXM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.166"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            72..881
                     /locus_tag="IVY61_002589"
     CDS             72..881
                     /locus_tag="IVY61_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7971650.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            921..1418
                     /locus_tag="IVY61_002590"
     CDS             921..1418
                     /locus_tag="IVY61_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7971651.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1803..1876)
                     /locus_tag="IVY61_002591"
     tRNA            complement(1803..1876)
                     /locus_tag="IVY61_002591"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1840..1842),aa:Ile,seq:cat)
     gene            2063..2386
                     /locus_tag="IVY61_002592"
     CDS             2063..2386
                     /locus_tag="IVY61_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971652.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2550..2804
                     /locus_tag="IVY61_002593"
     CDS             2550..2804
                     /locus_tag="IVY61_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971653.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            2874..3119
                     /locus_tag="IVY61_002594"
     CDS             2874..3119
                     /locus_tag="IVY61_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971654.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            3195..3560
                     /locus_tag="IVY61_002595"
     CDS             3195..3560
                     /locus_tag="IVY61_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971655.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(3621..4184)
                     /locus_tag="IVY61_002596"
     CDS             complement(3621..4184)
                     /locus_tag="IVY61_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971656.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         1486 a          828 c          787 g         1592 t
ORIGIN      
        1 tttttttaaa ttctcattta atcttaaggt aacaggaaaa tagatatgta aaaagaaagt
       61 aggaagataa aatgaaacta gcagcgattg atttagacgg taccttgctt gatcgaaatg
      121 gaaagatacc agaagaaaat atccaagcac ttcggaaatt tgatgaaaat ggcggaatcg
      181 taaccgtcgc taccggaagg aacagcattt ctgctaaaga tattttttcc gaattaggaa
      241 tcggtggcta tttgatttct tccaatggtt cactgattgc tgaaatgaag gatggaaaaa
      301 tggcgaaagt tctcagacgt tcaaaaatag atagcgatac actgagaaaa gccttcttct
      361 atgctcaaga agcgaaaata tccatcattg ctagccgaga aacccaagat gatcagctga
      421 cattcgatga atcaaatcta gtcaaagatg acccttatta tgaacatttc aatttacaga
      481 accattcttt tgatcaaatt ttaaatatgt tggaagatcc ttctcttagc tatcttaaat
      541 tagcattaac agataagagt gaagaaaaac tacagaagct acaaagagat ctgcaagcag
      601 atggtatcca atcagtattc tctgatccgc atttcttaga aatcacgcct acaggtatca
      661 ctaaagctca ttcacttgtt tttctggcgg attaccttgg aatcgaatcg agtgacatca
      721 tggcttttgg cgatcaagaa aacgacattg ctatgttgaa ttacagcggg ttgagtattg
      781 cgatggggaa tgcacagcga caagtaaaag aaattgcaaa ccaagtaaca ctgacgaatg
      841 aagaagctgg tgttgcttat attttaaaaa acttctatta aaaagaataa aatgacgata
      901 tgaatgtagg aggaaagaaa atgttagtta ttattgatat gcaaaatcat attcttgatc
      961 caaacagcga attttatatt ccagagtcag atcaattggc agaacgtatt gctcaaagat
     1021 tagctaaagc acgccaaaat aatgaatata ttctcttcac acgggatata ccagttgaac
     1081 tgaaggacaa agaagaagaa cgagaagatt tgcaattgat ccctcttctt tctccacaag
     1141 aaaatgaacg agtgattaaa aagtattact ttacgattcc acctgaaact ttagctgaaa
     1201 tccaggaaac tttatttgaa agcaaagaag aacaaaaaga gatcgaagta gtcgggattg
     1261 aaacaaactt gtgtgtactc tctaatctga taggcctgca gagtgctttt cctgaagcag
     1321 acttttttgt tgatccaatg ttagtctcca gtagaaaaca cggagaatcc gctttagagc
     1381 tgttgaaaga tttcaacgtc tctatcgtcg aatcataaaa atatctatct tcatttacga
     1441 tatttaacac acaataaaaa aagcacgcag aactgtttta gtcctacgtg ctttttctat
     1501 caaagtagat tggctttttt atgacacaga gtgaaacggc aggctcacga cctctttctt
     1561 aaatggtgtt caatcagctg accctcggta ttagcttaac aaacggcaaa atatgaggaa
     1621 ctatttttcc gtttgttaag ctaatactca ggtcaaaacg ctgatttcac aacctcttcc
     1681 ttaaacggta ttcaaaagct tgcatctgca gtaataagaa cagccaagta aaaacataag
     1741 aagctgtttt tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct
     1801 tatgtacctt gtaggactcg aacctacgac cggacggtta tgagccgtct gctctaacca
     1861 actgagctaa aggtacatca taaataaaat ttatttttgt tacttttcaa ctatagcacg
     1921 aatgacaaac tctcacaagt ttcaaaatat aagtaaaata atattgttat tcttatttct
     1981 tttacttgtg tcataactat tttgttactt taatttttat ttgcttattt tagaaaaaat
     2041 gttattcttt tatttattct caatgttagg agtgattatt ttgccattta ttgcgatttt
     2101 attcgattta gttgcggcag cagcttattt tctccaatta aattatcaaa cctcaggaat
     2161 tctgtttatt ggaatgattt ttcaaggtgt gattaccctt ttgcttcttg tcatgatgct
     2221 cagctataaa ggaaaaagat atgcccgtat tcaaacaaaa gtatttgtta aatatgtttc
     2281 cattcgctat gggatcatca ttctttcctt tttagtgaat gcgatcgtcc tctttttata
     2341 tgtattgaat tacttagata tcaatccatt gattttttct cgatagataa gacacattca
     2401 gcggaacagt tcctgcttat cagcagaaaa ctgttctgct ttttcatttt tcattgatct
     2461 attcttcttt tattttccta gcatttcatt tgtgatgtat gcaataacat gttatctttt
     2521 attatatgaa tcgttgagga gtccgttaca tgattacgtt ggtgagagtt ttgttttggg
     2581 tgcctgcggt cgcactggtc gcaagtattg tttatcttat gaattggaac aaagaacggt
     2641 tttatttagc tattttgact cttccggcta tctactttat gtggaaagtt tttaattaca
     2701 actactttga acccgatagt gtttttatag aagaattatc aggactggtt ttgtctttat
     2761 tgatcgtcat tctttatttg atacgtttaa ataaaaaaca ttgagtacta ttgattgcat
     2821 gtgtagcttt ctatagtcat tataactatt ctactataat gaaaggtgat taaatgagta
     2881 tcgaagaagc tattttactg gatactttac gttgtcgtct gaatttctta agaactggcc
     2941 gatctttatc tcaagtagct gtttctagcg cgttaagtat tcctttattc aaatatcgta
     3001 agtacgaaga aggagaagaa attccaagca tcattgaatt aatctatatc gctgactttt
     3061 acgatatttc cgttgattac ctaattggtc gctcagaaac agcaagcaaa ctgaagtgat
     3121 tttttccagc cttggtgctg gttttttcat ttaacgtcta aggttaacaa tctatatata
     3181 aaggagacat ttctatgcga ttatggcatg aaaaattgat ttcaaagctt cctcgtcaac
     3241 agcttttagg acaacatcgg gaatgctgtg cattaagagg aaatggctgg ggaaagaaac
     3301 atgctactgt ggattatgtt tttcactatt ctccttatag attattccaa tatcatcagc
     3361 ttgtcatgga tgaaatggaa aaacgtggtt acagcccagc accagagtgg aaaaatccta
     3421 tgtatcgtgg caaatcatgc gaagcatttt ttgatttacc tactgtagag cgcaacgtgc
     3481 cgatttatcc ggaacacgat gaagtttatc tagcagaatg tttagcaaat ttggagcaaa
     3541 aaggaattta tcttacctaa tataactaga aaagccttct atctcctctt acttcattaa
     3601 aaagctttaa ccatcttaga tcaagctaaa atgtcgtaag ttttagggtt atcttcatcc
     3661 tcacgtgcaa aactaaaagc gtactctagt tttggttcat cgccaacttc cctagacata
     3721 aatgaacatc tcctgacgtg gtttaaaagt accgcatctt cgacgtctgt atacgatgca
     3781 cagcacagat tgcttctttt gatctttctt ttgatttttc tgttgcttct tctacacctt
     3841 cttcgattac tacatcgatg cttacaattg cttctgccta atattcccat gcatcgtcga
     3901 tttaagcctc tgtagtcact gtgatcgttt ccacatttta actcctctgt cgtggcttca
     3961 gttgattttg tgatcagttc agttgctaag ccagagtcag aacaacctgc cattgctagc
     4021 gttaatattg atagtaaaaa tagttttttt atcataaggt tccccccctg aaaatagact
     4081 aaaataataa cgctttcttt ttttgttttc agacgaaaat taataatttc taaatattct
     4141 ttaaaaaagc ttataactat tatgcctttt ttaatatata acacagttat ttttaaaaat
     4201 aagaatgcta aatcactcta caataacggc gaaagcacaa aaaataaaaa aattattacg
     4261 ttattaaaaa aacagatgaa agatcaggtt ttattagatc tcattctttc atcacttgaa
     4321 gggatttaat gaactacttt ttcctctttg tcgtttttat atattcgtat tccttcaaac
     4381 agtaggatag cctctttcaa tgcttgatat ttgaactgtc cgttacttaa aaatttagat
     4441 gtgccgccct cttcctagaa catattcttt tttacttttt cattaattga cagaccaatc
     4501 gaacgaaact gtaaatttcg ataggataat gattggtcaa actcattcat tttcaagaca
     4561 tctcgattca tattcaagat aggaatcttt ttatcaacat cactttattc gtattttcct
     4621 ctaccataaa agcttcacgc tccacaaaat tgttttatac actactattc agcccattca
     4681 acgaccaatg aat
//
LOCUS       DACCXM010000158         4591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCXM010000158 DACCXM010000000
VERSION     DACCXM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.167"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            190..2859
                     /locus_tag="IVY61_002597"
     CDS             190..2859
                     /locus_tag="IVY61_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7971657.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2950..3570)
                     /locus_tag="IVY61_002598"
     CDS             complement(2950..3570)
                     /locus_tag="IVY61_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7971658.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3684..4223
                     /locus_tag="IVY61_002599"
     CDS             3684..4223
                     /locus_tag="IVY61_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971659.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4220..4558
                     /locus_tag="IVY61_002600"
     CDS             4220..4558
                     /locus_tag="IVY61_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAP7971660.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1423 a          790 c          860 g         1518 t
ORIGIN      
        1 aaaaattttt aagggtggga cttttgattc ctaccctttt tttgcgttta tttttgttat
       61 acgaacaaat tatgaagtta tttattctaa attttatttt tttgtgaaaa ttagactctt
      121 tttaagcaga gaaaaacaag gatatgataa actgaataca acttcataga aatttggtgg
      181 aaaataaata tgaaaaaaaa tttaaagtca ttctttagag aaaacggacg ctacgcagca
      241 gctagtttcc tgattccgtt tttgatttta gcacttgttt atctaagtat cgggatctat
      301 cctggaagca gcagaagtat tttggcaagt gatgcctttt cacagttttc taattttcat
      361 gccagcttcc gaaatgttct tcttggaaag caaagtattt tttatacatg gaatgcttct
      421 ctaggattga attatttatc tttaatttct tactatctag gcgggatctt cacgccactg
      481 gtgttctttt ttccaaacca gatgatgcca gatgcactgt atgttctgac tttgcttaag
      541 gtcggctgtg ccggattggc tttttggttt cttgcacgta cgttcaagat tcctcgctgg
      601 gggcatgttg cacttagcgt cagttacgca ttgatctcat ttatcactgc acattcggag
      661 atcatcatgt ggctggatgc atttgtttat cttccattag tgattttggg gattcatcgt
      721 gttatggatc ttaaacggcc gaaactttta tttatcagtt atttactctt gtttttatcc
      781 agtttttata tgggttttat gattggcgta ttttctttct tatattttat cgcccgtcta
      841 ttgacaaact ggtcagaata taaaaaaacg attgttcctt atggtatcac ttctttactg
      901 gcaggcggcg cttcaatgat cattgtattg ccagcagtgc tggatcttcg gtcaaatggc
      961 gaggcactga gtcagatcac tacgttgaaa acagaagcga cttcttatct ggatctaatc
     1021 atgaaaaata tgattggtgt ttatgacacg acaaaatacg gatcgattcc gttcgtttac
     1081 gcaggtctgc taccattagt cctctgtctg ttttattttg ccagtcggaa agtaccgttg
     1141 aaaaataaat ttttgtacgg cagtcttttc gccatactta tcgcaagttt ctatcttgtt
     1201 ccattgaatt tgttctggca tggtatgcat gcaccgaata tgtttttatt ccgttatgcc
     1261 tatctgttct ctttcttagt gctcctgctt gcaggaaaag gatgggaaaa gatagaacaa
     1321 gacgatcgtg ggctgttgtt aggaatcatt ttggtcgtag ctgctctctt tgcggtggca
     1381 tggggcatca aacctgaagg aagctacacc tatgtgacga tgacttcctt tgtattgaca
     1441 gtggttttct tagggattta tgcttttgct gtgggatttt atcagcataa aaaactttca
     1501 ttcaatcatc tgagtatatt gttattattg atcatgtctg ctgaagcact ggtcaatacg
     1561 aacagtatga tccatggcat tcttgatgat tggaattatg cttcccgcag tttatatact
     1621 gatccttatc ctgacttgaa agctcttgta aatgaaacaa aaaaagattc cgactcgttt
     1681 taccgcttgg aaaatctgga tccagtatct gcaaacgatt cgatcaacta tggttacagt
     1741 ggtatcagtt tgttttcttc tataagaaat cgtaattctt cctcttacct ggataaatta
     1801 ggtttccgtt caagagggac aaatttgaat atccgttata acaataatac tttgctaatg
     1861 gatgggttta caggaatcaa atataacatt tctaaaaacg aaagcacgtt cagtaaatat
     1921 ggcttccgcc aagctggtca aagtggcgac tatacactgt atcagaatgt caatgcattg
     1981 ccactagcat ttactgcacc gttgtccgtt aatgatgttg agcagccagt gacagataat
     2041 ttgaccagtc agacaaactt gatcaataca ttgtctggtt taaatgaaca gtattttaca
     2101 ttctatacac ctactttgaa gaatcagttg aatacgacga tcacaaagac aacgactggc
     2161 gtcacttact cagaaacagc agataaccag ccaaaagagc tcacttggga agtccatgtg
     2221 cctgcaaata cacaagctta cttaagtttg ttcccaacca attttgcaca attagaaagt
     2281 tctagtgcga cgatcactgt caatggtaca tcgcgaaaaa cgcaaatcaa catttctggt
     2341 caatactatg atcttggcta ttatcctcaa gagacaacag tgacatttac tgcaagtttt
     2401 tatggaacga aagaagtaag tttcatggag cctaaagtcg ttggtctaga tgttgtagcc
     2461 tatcaagccg caatgaatca gatcaaagaa aaaggtgtcg aaatggaagc cactggcaga
     2521 aaagcttctg ggactgtaaa taccgctgaa gattccatgt tggtgacaac tattccttat
     2581 gacaaaggat ggaaagctaa aattgatggg aaaccagtca aggtcagcaa tttcaaagac
     2641 gcattcttga tgataaaagt tccagcaggt aaacatactg tcactttcag ttacttgcct
     2701 gaaggattta cgataggtgc aatccttttt gtcctatgta ttgcattgtt tattctttat
     2761 gtctggtacc ttgaccgcaa tcgccccttt tatggcacga ttgatccagc aatagaagaa
     2821 aaaccgcgaa atacacacag aagaagacga agaaaataaa gaaacgatgg aatctgtgtc
     2881 actcttctat ttaaatggca aaaatttcaa tctttaaatg acaaaaacca cctgattttt
     2941 ggtggttttt tatataccta tgactaaact atctcttgct gcttcgacaa cttctacagt
     3001 gatcttatcc atttgattga ttcttaaaat ccgatcaatc tgcgcaaata aacgatgaat
     3061 aagcctaaaa tttccttttg tgatcttaat gatagttgtg atagcctcat aatcagaaaa
     3121 atcttctagc ttgatatcaa ttcctaaatc tgcccatttg tattccaaaa tatgatgtgt
     3181 ttcgtcctta ctaaggttat caaattcatg tgcaaatcct atgcgagagt aaagctgcgg
     3241 ataacgagac aatctttttt caataccagg cataccgata agaaccattg ccagatcatt
     3301 tctatcgtaa atatctctta gctgttctaa atgctgaagc tttaaacgat caatttcatc
     3361 gacaataatt aagtcaattg cttcataggt tttaggcaca ggaggatctt cttgattgta
     3421 ttttagtacc atatgcattg ctttagtcag acctattcta ctagttattg tgttaatatc
     3481 cgaactgaca cgagacatct tttcagctgg tacagtataa aaaactttat ttacacttaa
     3541 tatttcatct gttgcattta tcaagaacaa gaaaaagtct gaatattcaa aaaaaataac
     3601 ggtgtacatt tttaaaagga gatgtataat cgagatttag aaagcgtttt cctcaaaatt
     3661 taatgaatta ggaagtgatt actatgaaaa aattatttag cgcacttttt attctgttat
     3721 ttggctttat tggtattggg acttctggaa atatagttaa agctagcacg atggttgaac
     3781 aaacaagtga aatggcaaaa acttttagtc aattaacacc agaagaacaa agttacttca
     3841 aatccaaagg atttagtgat aaagatactt tttacactca aaagatagag cctcaagatc
     3901 cgaaaacacg tttttctgca aatgtattgc ggttaaccgc atctactaaa aaaattacta
     3961 atacgcaggg gcggacagaa tatattataa caagtcccaa ttcggcgatg tatcgtattt
     4021 ctacacgctt gagtttaggt agtgcaaaat catatactca aacagtaaat ctagctaatt
     4081 cttatggtta tggtggagga atgtatttta catttactgg gaaaagacaa tattacagtg
     4141 taaaattagc tatgcaagtt tataatacat ttggtgtagg gtcagtctct tgcactgcgg
     4201 gaggattgac aataggaaaa tgataatttc ttactatgtc ttaaatagaa gtgatgatga
     4261 aggactcttc gtttttcaat cattacatga ggatacgatg ctaaaagagt tttataatcg
     4321 gattaagaat aaacagatag aaacaaattt atatttttta agtgactcta tcttgccttt
     4381 tattagcaac cctaaaattc ttaacttgtt gcagacaaaa gatttgaaaa gtttaccgat
     4441 tattgtcatt gatgaggaaa ttgtaaaata tggcgcttat ctaagcgtag aagaattaga
     4501 aaaattaact aacattggaa tgagtattca ggaggataat ggggtacatg gaacatagaa
     4561 tgaatatgca cagttgagaa cggaatttga c
//
LOCUS       DACCXM010000159         4530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCXM010000159 DACCXM010000000
VERSION     DACCXM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.168"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..955)
                     /locus_tag="IVY61_002601"
     CDS             complement(<1..955)
                     /locus_tag="IVY61_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7971661.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGG"
     gene            complement(957..2396)
                     /locus_tag="IVY61_002602"
     CDS             complement(957..2396)
                     /locus_tag="IVY61_002602"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP7971662.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2581..4527
                     /locus_tag="IVY61_002603"
     CDS             2581..4527
                     /locus_tag="IVY61_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP7971663.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1428 a          780 c          756 g         1566 t
ORIGIN      
        1 ttcctccaga aagtattgag attggtaaaa tataatcctt atttgtcttt tcgcctttaa
       61 tttttttcag aagaatatca acaataagac ttgcaatttc atcaattggt tgtcttattg
      121 ttgtcaattg tgggaaaaat ttctcaataa aagaggtacc atcgtatcca ataattttta
      181 aatcttctgg gatattaagt ttaagttgat gagcaatttt catagtgaga atggctgtta
      241 aatcatcaga aacaaaaata ccatctggtt tgtgatttat aatagttgac tttatttcca
      301 tttcacgacg gatagtagac agattattag gaattttgaa aatttttcca tttggatttt
      361 gaaaagaaaa acctagtgca cgtaattctg tgggagaatc cgtgttatca tttcctgtaa
      421 tcataatggt attttgacaa ccattttttt gtaaagtctt agctgcaagt tttccccctt
      481 caaagttatc tgaagaaaca atggaaacat taggagctaa atttctatca aaagcaacaa
      541 taggtgctct cacacgctca taatcagcaa ttccaagatt atggcttgat gagattatgc
      601 catcaacttg gttggcttca agcatttcaa tgtattctct ctctttagca ggatcatttt
      661 gactattgca aataatcgtt ttatatccat gtttaaataa ctctatttcg agatactcaa
      721 tgagttcaga gtaaaagata ttactaatat taggaaaaat aagaccaata agctgaggtg
      781 actttccttg aagtccacgt gctaaattat ttggtttata accaagagtt ttcatagctt
      841 catgaacttt ttgttttgtt ttttctgata aatatccctt attattaatg acgcgtgaaa
      901 cggttgttgg gctaactcca gctaattcag cgacatcagt taattttgct accattttaa
      961 tattttaatt caaagtattt tccagtaggt tcgcctgatt tgatttgaat accattttgg
     1021 tgattttcag ggaatacacg actagtaaac actttttctc ctttattaat aaaaatctca
     1081 atgattgagt tatctacaaa aatgttagca gttgtttctt tagtatcaat tgagcattca
     1141 cgaatcgtac caaagtcagt ggcatattga atgcctgctt tgctgcgatc taaaataatt
     1201 tttccttctt tggtatctac aatcaagcta agtccatttc ctttactatc agagaaaaga
     1261 agtaactccg atttctgatt tggaggaaaa gttagttcaa gctcataagt attatttgtt
     1321 tctgcttttg ctgtaaattg ttcagaacta gcacgtaatg atgtaatagc ttctactgga
     1381 tattggtaaa gtttaccatc ttttattgaa agttctttta caagactcat tgctccctga
     1441 taatcatact tatcagttgg ataatcgata tcaggtagtc caatccaact gacaatcaga
     1501 gcacgaccat caggagtatt gaatccttga gtcgcatacg cttcaaatcc ataatcaaga
     1561 ttttgaattt ctgaagcgcc cacaagtttt actttttcta tgtcaagcga ttgacagatc
     1621 ttaaaagtat tcggatagat attcccataa tttagctcag atttatccaa cccttgagga
     1681 caataaatga gaacaggttt tttgtctaca aatactagat ttggacactc aatcatgtat
     1741 tctgaaccag tgccgccaaa atcaaggttg ccaacttcct cccatgtttc cacgttattg
     1801 tcatccgctt tgtaaagttt gataaagcct gatttttcta aattttgtgc tccaacaatc
     1861 gcatacaatt gtccttcata attaaacatt tgtggatcac gaaaatgatc agttacatca
     1921 gcaggtttat gaataagaac attctcacct ttcgtaatgt tgtagtcttt atccatccat
     1981 gctccgattt gaagtgtatc acgttcccaa ttttcatttc gaacgtttcc tgtatagagt
     2041 aagaatagat tcccctctat ttcatatgca gaaccggaat aagcgccgtg gctatctcgt
     2101 tttgtgtctg gaagaagttc tgctcctgtt acatgaaaat gaactaaatc atctgattca
     2161 ttgtgtaccc attctttcaa cccatgagca gctccaaatg gccaattttg ataaaaaagc
     2221 gtatattttc cgttaaaaaa agaaaaacca ttaggatcgt ttaacaatcc tgttttaggt
     2281 tcgatatgat agctagcacg ccaaggtgat cgttttacat tatccttaat tttatccatc
     2341 tcatctttag tccaatcaga ataagcacga tatcgtattt ctcgaggtaa gttcatattt
     2401 tactccttca atttatcttt ttacaactag tatggtaaac gttttccgtt gaaaaatcaa
     2461 tcatttcatt taaaaaaata aactatatga caaacgtttg acatattttt aagatatgct
     2521 aaaataaatt tgcatttcag tgaaccgttt tcacgtaaaa ataaaaaagg agtgattcga
     2581 atgaattaca gtaaagtagc ttccgacgta atacaagcgg tcggaaaaga taaccttatt
     2641 gcagctgcac actgcgctac acgtcttcgt ctcgtactaa aagatgacac gaaagtgaat
     2701 caaaaagctt tagacgaaaa tccagatgtg aaaggcacat ttaaaattga tggacaatat
     2761 caagtgatta tcggtgcagg tgatgttaat ttcgtttatg acgaacttat caaaaaaact
     2821 ggactttcgg aaatttcaac agatgattta aaacaaattg ctagtaaaga taaaaaattc
     2881 aaccccatta tggcattaat taaattatta tcagatattt ttgttccaat tatacctgcc
     2941 cttgttgcag gtggtttatt aatggcatta caaaactttt taacagctgc tggacttttt
     3001 ggaccaaaat caattgaaga aatgtatcct gcgattgaag gtatttcctc aatgatccaa
     3061 ctgatgtccg ctgcaccatt catgttctta ccaattttag taggtatttc agctgctaaa
     3121 cgttttggag caaaccaatt tcttggtgct gcaattggta tgatcatgac aacaccagat
     3181 cttggtggct caactgaata ttggaatatt tttggttatc atgtttcaca aacaaactat
     3241 gcttatcaag ttattccagt tttagcagct gtttggatat tatctatatt agaaaaattc
     3301 tttcataaaa aattgccatc atctattgac tttacattca cgccattatt atcagtaatg
     3361 attactggat ttttgacttt cacagttatt ggccctgtaa tgttattgtt gtctaatgga
     3421 attacagaca ttattgtttg gctatataac actactggat ttattggaat gggtattttt
     3481 ggaggaacat attcacttat agtcatgact ggtttacatc aatcatttcc agctattgaa
     3541 actcaattac tttcagcgtg gacaaatggt atcggacatg gagactttat ctttgtagtt
     3601 gcatctatgg caaacgttgc tcaaggtgct gcaacatttg ccatttggtt cttaactaaa
     3661 aattctaaga caaaaagtct tgcttcctct gcgggtgtct cagcacttct tggaattaca
     3721 gagcctgcct tgttcggagt taacttaaaa taccgctttc cattcttttg tgctttaatt
     3781 ggttcaggaa ttgctacagc agtagcagga ttacttaaag tagttgctgt ttcacttggt
     3841 tcagcaggtt tcttgggatt tctttcaatt aatgcaacat ctattccatt ttacgtattg
     3901 tgtgaattga ttagctttgt agttgctttt gcaattactt acttctacgg taaaacaaaa
     3961 gctgctggta tctttaaagc agaagaaatt gctgaacaaa caccagttaa ttcttcaaaa
     4021 gtagcaacaa cagatccaat atctactgat gttgaatcaa cacaaacaaa agtttcatca
     4081 gaaacaattg ttagtcccat ttctggtaaa gtgattagtc tttcttcagt aaatgatcca
     4141 gtcttttcat cagaagctat gggaaaaggg attgccatta aaccaaatgg aaacacggtc
     4201 cattctccag tagatggtac tattcaactc acgtttgaaa ctggacatgc ttatggtatt
     4261 aaatcaaata atggtgctga agttctaatt catattggta ttgatactgt atcaatggga
     4321 ggaaaaggat ttacacaaaa agttactact aatcaaaaag ttagaaaagg cgatattctt
     4381 ggagcatttg accgttcaat gattgagaaa gaaggtcttg atgatacaac aatgattatt
     4441 gtgaccaata cttcggatta ttcagaagta aatatagtag ctgaaaatac agtcactgag
     4501 ggtactgatt tacttgaaat caaataaata
//
LOCUS       DACCXM010000160         4430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCXM010000160 DACCXM010000000
VERSION     DACCXM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.169"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            63..1397
                     /locus_tag="IVY61_002604"
     CDS             63..1397
                     /locus_tag="IVY61_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7971664.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1438..2349
                     /locus_tag="IVY61_002605"
     CDS             1438..2349
                     /locus_tag="IVY61_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7971665.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2346..3200
                     /locus_tag="IVY61_002606"
     CDS             2346..3200
                     /locus_tag="IVY61_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7971666.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            3197..3814
                     /locus_tag="IVY61_002607"
     CDS             3197..3814
                     /locus_tag="IVY61_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7971667.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            3816..4334
                     /locus_tag="IVY61_002608"
     CDS             3816..4334
                     /locus_tag="IVY61_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7971668.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1327 a          758 c          900 g         1445 t
ORIGIN      
        1 gaacaaaatg atatcgttat attaaaaccg gcatcggtta cattaaaatt tggaggtaaa
       61 atatgaaaaa attattgttt acagggttgc tggcaagttc agcattgttt ttattaggag
      121 gatgcggatc aagtaatgca agcgatgcca gttcaagtgc agcaaattct tctgaaaaaa
      181 caagtgatgg aagaaaaaat attgatttct ggtccttttg gggatccggt gcacgaaaag
      241 aagctatcga agaaatcatt gaggatttca atcaatcaca aaacgagatc catgtaaatt
      301 ataagtatca gccatggggt gacatctgga ctaaatcttt atctgcaata acagctggaa
      361 atgcacccga tgttattgta caggacataa attccgttgc ccaaagagca gaagcacagc
      421 aagcaactaa tctctccaaa tatgtagaaa aagacacttc taaggatttt tatccgcagc
      481 tttgggatac agtagaatat gaaggtgatc cttatgcgat cccattcaat acagatacac
      541 aagtcatttt ttataataaa aagttattta aagaagctgg aatcagtgaa caagatctac
      601 cgacaacttg ggatgaatta gaagaaacag cacgtaaact agataaaaaa gaaggaaaag
      661 aatttacacg catcggtttt tatccattat ggaatttagg ggctgatgta tgggcgctaa
      721 acgcagataa cgggactagt tggtttgaca aagatgaaaa agtcaagatt gatactaaaa
      781 ataaagtgga agccttggag tggatccaag actggcaaga ttattatggg aaaaatacga
      841 tcaataaatt agaagccgaa tttgggtctg gtgtatctga tccatttatt tccggattag
      901 tagccatgcg tgcccaaaat atcaattatt actctagttt gatggaaaat gcaccggatg
      961 attttgattt tggcgtgatg cagctcccag aaaaagaaga aggttcaggc cattggtcgt
     1021 ggggtggcgg attcgtcttg gaagtaccat acggagcaaa agatcctgag gcctcttatg
     1081 aattcatcaa atatctttct tctccagaag tacaagaaaa attcggcgaa aaaagttttg
     1141 atatcatggc gagcaagaca gccaatgaga atttagtgaa taatgacaac ctaacggata
     1201 aaggaaaaat gatctatcaa atggcggacg aaaactttaa aaatacggtg atcacaccag
     1261 ttcctttaga tgctccggat tacacgacat tagtgaatga acagattgac cagattcttt
     1321 taggaacaaa atcaccaaaa gaaggattgg cagatgcaca aaaagcagta gaaaacttag
     1381 tcaagcaaaa tcaataatcc atttttgact gtctaggtac agaggaggca agaaaatttg
     1441 aagcgtttat ctatccgaaa gaaaaaagaa gcaagatggg cctatttatt tatctcgcct
     1501 tttattatcg ggttcttggt atttatgcta gggcctatgc ttttttcagt tattggtagt
     1561 ttcaccgatt ataatttgac atccaagatg aatttcattg gtctagcaaa tttcaaaaga
     1621 atgttcgttc acgatgattt attttggaaa tcgttataca acaccgttta tttcgtcatc
     1681 ttcaatgtac cgctgacgac gattttcagt gtgtttttag ccacagtact caatcaaaaa
     1741 atcaaaggaa tcagtgtatt ccgtacagct ttctatttac cggcagtctt atcaggagta
     1801 gcagtctaca tcttatggat gcagctgctt tctccatctg caggattgat caacacattt
     1861 ctgggctggt tccatatccc tggaccagct tggctgtttg atccacaatg gacgaaacct
     1921 gcgttgattt tgatgaaaat atggagttct ggaggagcaa tgctacttta cttagctaca
     1981 ttacaaaatg ttcctagatc tttatatgaa tcagcagaaa tcgatggagc aggcgtttgg
     2041 ggaaagttca aaagtattac tttgccatta atcactccta ttatttttta cgatgtgatc
     2101 acctctacga tcggttcttt ccaaattttt caagaagctt atgtcatgac gaaaaatgga
     2161 acaggcggac ctgcaaattc tttgcttttt tataatctgc atatgtggaa caaggcgttc
     2221 gtagcttttg atatgggtta tgcgatggct atgtcaatga tcctgtttct gatcgtgttg
     2281 gttctgacgt ttgtcaattt gaagcttgca cctaagtggg tctattattc tggaggtgac
     2341 ggcaaatgat ggattatttc aaaaagacca gtgtgaatcg gcaaacaaaa aaaatactaa
     2401 gttatgtcct catgacagta ataggaatca ttttgatcat tccattgctt tggatggtgt
     2461 ttacttcatt gaaaccaatg gaagaaatcg ttcgttatcc tccgaccttt ttcccagaaa
     2521 aaatcgtatg ggaaaactat ctggatacga ttgccgcctt tcctttttgg cgctatgcaa
     2581 gaaatacatt attcatcact gtgctagtag tgatcggtaa tgtcctttca aattctttta
     2641 ttgcttatgg atttgcgaag cttgattttc ctgggaaaaa gctgatgttt gctttggttc
     2701 tttctacgat gatgatcccg gggtttgtaa cgatgatccc tcaatatgta ttattctcca
     2761 agatcggctg ggtaggtact tatttgcccc tgatcgttcc atcgttcttc ggcaatgcgt
     2821 tcaatatatt tctgatgcgt caattttact tatcgatcaa taatgaattg atcgaagctg
     2881 ctgaaatcga cggagcgaat catttgtata tttggagtca tttgatgctt ccattgacga
     2941 aacctgcctt gattaccatt gcgatcaatt ccttcaacgc agcttggaat gatttcttag
     3001 gtcctctgtt atatatccaa gatcaagaaa aatacacgtt acagattggc ttgcaagttt
     3061 ttcagaacca agcaacgaca caatggaatt atctgatggc aggagcaaca cttgtcttga
     3121 tcccgacgat tttgctgttc ttttttgcac aacgttattt cattgaaggg atggatttga
     3181 caggaggttc aaaaggatga tgaatcgatc gattggtaaa aaagccaatg attatttctt
     3241 agaaatcatt gaatggattc cacgtcttat ccatttacag ctactatggt tcctgtgcgt
     3301 cttgcctgtt ttcacattag gaactgcaag tcgaacagtt ctgtatatga tttatcatta
     3361 caaaaaaaat gaagagaaaa agctcggttc actattctgg aaaaagtttc gtgagaactt
     3421 ctctctttat ggaaagcaag atagtttagc aagtgtttat tttcttcttc tattgatcga
     3481 ttatcagatt ttccgctatt ggggtggggg aattggctat acattgatgt atactacgtt
     3541 gtttttgctt ctattaagcg cgcttctctt tagttacaaa atcttgcttc aactggaaca
     3601 aaaagaagct tcttggatta ctgcattttt cttgtttttt aatgatttca aaagcgcgct
     3661 tttctatttg gcaggcacgt tgatccttct tgtcgcattc tggtttgcag gaccaatcta
     3721 ttttgcttta ttaggtttca gtttcttgtt ttattttcaa gtcctgcttt ttatccatcg
     3781 tacgcaagaa aaggttctta aaaaggagga ataacatgtt gcgtttgatt gctaaaatcg
     3841 atgatagtat tatcaacaag ttcgccaagc aacggaaaac ggatagtcga aaaatcaatt
     3901 ttttgaaatt tatcagtaat atctctaatg gtggttttat ttggttgctc cttgctgctg
     3961 ttttagtctt gatacctgat actagagaat atggtctagc cattcttatc ggcagtttga
     4021 tctatttttt tgttggtttg ctttttatca aaaaaacgac aagacggaaa agaccttatg
     4081 aaaaaaatcg tcggcttgtc ctttacacaa gtaaaccgaa gacttcttct tttccttcag
     4141 gacatacagc gctagccttt ttatcagtag ctgcgttgtt cgaattaagt ccagttgcag
     4201 gaatattagc ctttttattt gcttgtttga tcgcttattc gaggattttt ttacttttac
     4261 attatcctac tgacatttta ggaggagcta ttttaggaag tgttattggc atcctatgcg
     4321 ctgccgttat ctgaattaat tgtacgaata aagcatgagt gcgtaaatgc gcatttgtgc
     4381 tttatttttt tgtttcgagg aaagcgtagt tgtttattca tttgtcttaa
//
LOCUS       DACCXM010000161         4398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCXM010000161 DACCXM010000000
VERSION     DACCXM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.170"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(151..636)
                     /locus_tag="IVY61_002609"
     CDS             complement(151..636)
                     /locus_tag="IVY61_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HAP7971669.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(638..1402)
                     /locus_tag="IVY61_002610"
                     /pseudo
     CDS             complement(638..1402)
                     /locus_tag="IVY61_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1572..2765)
                     /locus_tag="IVY61_002611"
     CDS             complement(1572..2765)
                     /locus_tag="IVY61_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7971670.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(2849..3052)
                     /locus_tag="IVY61_002612"
     CDS             complement(2849..3052)
                     /locus_tag="IVY61_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7971671.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            3309..3551
                     /locus_tag="IVY61_002613"
     CDS             3309..3551
                     /locus_tag="IVY61_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971672.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(3473..3706)
                     /locus_tag="IVY61_002614"
     CDS             complement(3473..3706)
                     /locus_tag="IVY61_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7971673.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(3713..4144)
                     /locus_tag="IVY61_002615"
     CDS             complement(3713..4144)
                     /locus_tag="IVY61_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7971674.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1105 a         1079 c          847 g         1367 t
ORIGIN      
        1 tatcatagag ttttacttat ctatagaatt atgtctgttc ctaattcagt ttgatgaatt
       61 aactgataaa aaaatatcta ctatttaaag atacagggat aagtaagtaa aaaaataaca
      121 tgtcattaga tagtagacag ttccaactct ttatttacct tttttgtatg ctgcactgtc
      181 aatccaagtt cctaaattgt tgtatccatc atcacttata taatcatatt ggccatcaat
      241 aatttcgtca aaccacaaat attctccatc tggtttttgc ccaattattt taattactga
      301 ttgactagta gttgtcatct ggttaaaatc ttttatttta cctatattaa cggccaaaat
      361 agcatttcct cgcttataac tctcctggat ttcatattta acatattctc gatcaagagt
      421 ttctttacct atcagcacca cagtcactga tgttccaatt aattgattat ctatccagtt
      481 ttttatagca atatctccat tacgtttaac tttctcaaat tcagccttat ctacaaaacc
      541 agcttccttt gttcctcgca aagtccagct gtttctaact accattgctc ttgttacatc
      601 tgcataatgg aaactaaaaa aaacccttct caccatatta atcctcctga atactattta
      661 ttaaactgac aatttgttca acttttttat ctacttctcc ttcagtatct aatttttcta
      721 gcgcttgaaa tttacttttt aaggaatcat caaaataatc actgaaattc tcttgaatct
      781 tatcaaacaa aaccttactt gcacctttgg taaatccaat cggaactact atattttcat
      841 tttgaacaga taattcaaat tcttcaatca ttccatctgc tgtaactatt tcacctgatt
      901 caagtttgtt tccaaaaaca aaaatactaa tcccagactc tgcaataatg ttttctcgat
      961 atcttctcca ctcatgaggc atatcaaggg gaagtggtct taaagaaagt acgtcttgta
     1021 tgttattttt tcttaaattt cttctctgct ttagaactcc tgaaacaata ctattaccaa
     1081 ttccaagtcc gtaacctgat actattctat aaccggattc agaaagccta tatgctaatt
     1141 tctcgacaaa ttttaatcct ttcgattcag atgtaaatgg agtataatca acagcacttc
     1201 ccgaaataaa aatagttttt ctcaaatact tttttctaat acttttcaaa atatcagtaa
     1261 tctcagaata atcatctact tcaacaacgt ttatcgaaaa tctggacaaa tcattgattt
     1321 gtagtcttct ttttgtcagt tcataattat atctctcttg atcaaaaaaa tcctcagaat
     1381 cgacaaaatt ttcctttaaa attatatggg attttagatt atctttttag aagttcttga
     1441 atgtcggtat agcccgtgtt tccgggcttt tccggcattt ttgatattgt tagatactta
     1501 caaatgttct tattacctct caccatttcc ccctcaaaag tagtaaatgg agtagtaaaa
     1561 ccggccttgt gtcaggctgc cagccgttcc atttctgccc ttgcggaatc gaatgtggcg
     1621 tgtgcataat agttcagcgt catggtgata ttggaatgtc ccataatgta ctgcaaggct
     1681 ttggggttca ttcccgcatt ggctaaccgg gtgcagaatg tatgacgcat agcatggggc
     1741 gtcgtcgttt tcggcaacgc ttcttcatgg ctcttgttgt acttcttcac aagcctgcca
     1801 aacatcccgc cataatctac cgccgtcatg ggatagccgt tctgtttgag aaacaggaaa
     1861 ttggcatacc cgtcaataat gaacggcttt gcacctttgc ggttttttat cactcgctgg
     1921 aacgcctgat acacttcctc attcatgggg atttttctga cgccgctttg ggtcttgggt
     1981 tcgtcaatat agtaaccgac gccggtattt ctcaaaagct ggtggctcac gatgattacc
     2041 cggttttcaa aatccaaatc tttgtccgtc agtccgcaca gttccgaaat gcgaagccct
     2101 gtccccagca gaataacgat tgcgtcataa tgcttttggt aaaccttatc accttgaata
     2161 aaggacagaa aactttcttc ctgtgctggt gtaagaggca cctttggttc cgtatcgtcg
     2221 tccagaacgt cactaagctg gaagtcaaac gggttttttc ggatgcaatc gtcctgtatg
     2281 gcggtgtaaa aagccgcttt cagggaacgc ttgtcgttat tgatggtctt ataagaaagg
     2341 cccttttctt tcatccgaag cgcccattcc tttgcgtcgg acagcttcac actgtcaatc
     2401 gggcagctcc ccagccgatc ttgttccaga atacgcatca gccgttcccg gcccagtttg
     2461 gtgttgtgct tcacattgcc ccggtgcctg atctgcttgg cgtagagctg gcagacggtc
     2521 attttcttgc cgatggtgtc tatcccgtcg tcaaggtctt tctggatttc tttctccttt
     2581 tcccggagtg aaatatcctc ccgtttacct gccggggtct tatccgtagg cactaacttc
     2641 catgagtaaa caaactgcgg ttttccaaag gtatcaacat atttgtaagc atatcgtccg
     2701 tcttttctct ggctctctcc agaccgcaaa acgcgatttt tgctgtctcg tcttttttct
     2761 gacatctcgt catgctcctt tccatgacgg aaagagccgc atatatgctt ataaattatt
     2821 ataacatatc tgcggccctt tttcactatt agattgtgtc caaagtgtca attattttct
     2881 caaactgctt gcgtttgatc tggatgcggt tgccattcat aatgacccag ccagaggccg
     2941 gattttcctc ggccagctta cgcaatttgt tttctccgat acggaaatat ttagacgctt
     3001 cctcaatcgt cagcgtgtat ttttcccaaa taggcacatc atggttcata atgcagcccc
     3061 ctttcacaaa ttcaaaggtt tgtgtgattt aggggcatag acggacggct gctggcacag
     3121 ctccacggga ctttcacccc cgcgtggttc tcacgcggcc ctacccattg cctgcgacgc
     3181 ttttaacgct cggactgtgg ctataaggga gtatcatagt cctgcctgta cgtcgtcgcc
     3241 cgcaagccgc cacgctcaca ttgcggcgtg gtgttcctcg ctccgctttt cccgtaggga
     3301 aaagtatcat ggcgcaccag ccgtccggct cggcacagac agaatgtgtc cgtatgccct
     3361 atgatgcgcc gccgttatct tgcgggcata tttatcaagg tgcgatcagg ccaccgggaa
     3421 aacaccgaaa cggggaaagg gtaagccgtt tcatgctgcc ggggccggtt cgtcagttcg
     3481 tgacgatagc gtggatgagc ttgatttcca gacggcgctt catggcctcg tccagacaca
     3541 catgcggata gccgttttcg tcatagagcg tccgggtaca cagcttgttc atgtaaccgc
     3601 tgtaatactg caaaacgcgc tctacggctc cggcatcccc agcgcgggcg gcctcgatca
     3661 ccgaaagggg caaaagctca atgcccttgt agttcgggtt cttcatccgc gtctaacctc
     3721 ccttcggcat caacgccttt agctgtttcc gcagctccga taaggtattt gtcctgtgcc
     3781 gctgaacggc actccgggac attcccacaa ggccgccgat ctcaccatct gccatatcca
     3841 gaacacagtg caggattaaa atactctgct ccatgctggg cagcgcggca aaggcttcgg
     3901 caacaagctc attgttgata tgtaggtcat agccatgcga cgaaaacgta aagctgtcgc
     3961 tggggtagtg atctactgta cagagcttgt ccaaatctgc ctgtgacaaa gagcttagtg
     4021 acacttcacg gtcacggcgg cgcttcattt cagccaagta gcttttggcc tcgttacgca
     4081 gtaccgtctt gcaaaaagca tcgaacatgc accgcgcttc atagtcgcgg gggatagcat
     4141 ccatcttctc accccctttc tgtgggggac ttaggtggtt ctctcccttt ccgctaacaa
     4201 gactttgaca aggccgccag ctacccgcta aatacctcct ttttgaaaat ttctcaaaaa
     4261 atttttttgg ggacagccgg gccgcaaaag acgacaaaaa tcgcccggca ggtcgcaaac
     4321 ctaccgggcg agaaaatgct ttgctatgct gctgatttac atggtatagt acatactcat
     4381 aaccggaata gccctgtg
//
LOCUS       DACCXM010000162         4350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCXM010000162 DACCXM010000000
VERSION     DACCXM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.171"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(68..964)
                     /locus_tag="IVY61_002616"
     CDS             complement(68..964)
                     /locus_tag="IVY61_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7971675.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(957..1637)
                     /locus_tag="IVY61_002617"
     CDS             complement(957..1637)
                     /locus_tag="IVY61_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7971676.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1638..2420)
                     /locus_tag="IVY61_002618"
     CDS             complement(1638..2420)
                     /locus_tag="IVY61_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7971677.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2413..3330)
                     /locus_tag="IVY61_002619"
     CDS             complement(2413..3330)
                     /locus_tag="IVY61_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7971678.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3494..3817)
                     /locus_tag="IVY61_002620"
     CDS             complement(3494..3817)
                     /locus_tag="IVY61_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7971679.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1276 a          949 c          756 g         1369 t
ORIGIN      
        1 cttttttctc tattaaatat atataagata atacattttt ctactagttc tcactaatct
       61 tgtataatta aaccctccgc aacttgacgg agatggagaa gatgcccccg tctaatctag
      121 cggtcatctc tcctcccatt ccttccacca atttttttga caggtaaagc ccgattccgc
      181 tagattccgt atttgtgcgg ctggtatttt cggtataaaa acgctggaag actttttcaa
      241 cagaggtttg cgttcctggt ttgataggat tggccactgt taatcgaatg aatggatcct
      301 cttttgaaaa tgtcaccttt gcttgtccat ctgcataacg caataaattc tgtatgatat
      361 tttgcagcac tcgcagcaac atgagcggat ctgctaatac agttgtattt atcgagtgtt
      421 cagcgaacgc tacctcgata tttttctcag aaaattgttc ataaaaagag aaaaatactt
      481 cttccacctc tttagaaaaa gaaagcgtat ggagatccat ttctttttgc ttcgtctcta
      541 ttcgagcaag atcataaaac tgatccgtca tactgagtaa tcttgcaaca gattgtttga
      601 tgatcgttaa atacttctcc cgtttctccg tatcctctac ctgttccagt aattgtacat
      661 aaccgttaat agcagtcaaa ggagttctga gatcatgaga aaggccgacg atcgatagtc
      721 tgatgttttc ttccatttct tgaaatgctt gtcctttttg ttcattttca tccaaccatg
      781 tattcagtgc atctactagg tccattagat tcttttctcg aaattcgact gaaagacgac
      841 caccatattc ttgtcgttca gaaagagtgg cgattttctt agttaatctc ttgatttccc
      901 tttgcaggaa gaaaataaaa cttcccataa agataacaac gagaagcaac agccatttaa
      961 tcaaatctaa accccactcc ccacaccgta tgaatataca cagcatgtgc ctttgctagc
     1021 ttccctcgaa gattggaaat atgcatattc actgttttct cactatcaaa atatggttct
     1081 tgccaaactg tttcataaag atttgctcgc gaaaatactt ttttcggatg ttctaaaaaa
     1141 agcttcagca gatcgaattc tcttcctgtc aaatggacag ccacaccatt cacatttaca
     1201 tccctcgact ctttgtccag taagatttcc ttgtaataga tgcgttgcga cagatctttt
     1261 acttggacag aagtctgatg acgaagttgg atgccgattc gtaacaaaag ttcttcgata
     1321 tcaaatggtt tcgtcacata gtcatccgct cctgctgcca acaaggaaac tttatctctt
     1381 tgatcgcttt ttgctgaaag aacgatgatc ggaacattcg atgtctgtcg gatttctgct
     1441 aacaatgctt cacctgaaag ccctggaagc atgagatcta gaaggatcaa atcgacagga
     1501 tatgtgctta acaataattt tccttccgta cctgaataag catcaagaag tttgtatctc
     1561 tgttcaagaa cctcctccag taaggaatga acagcttcat cgtcttcgat aatcaaaata
     1621 gtttccagca ggaccaccta cttaatgtca gatttttgga aaataaacat tcctagagct
     1681 gatgatacca cgattgttac tagactgacc agaatcattt tcggtgcttg cgaataagtt
     1741 gcttgttgcg caaagtccgt atacatgagc cacgtttccg taaaccactc cgttagaaat
     1801 tccaagcctt tgatttttgc gaaaacaccg ggaatgaagg ccgttcccat gatataaaga
     1861 aaagaaagaa gcaaactgac actggtactt ctagtgatga atgtcaaagc cactggaaca
     1921 gataatgtcc ctaatgtcag cagaagcaat acaggaaata cttttagaac ataatttcca
     1981 tagtttttga ttccctcaac ttcaccaaat ccgaaagcaa gtgtgtaagc aagcatggct
     2041 agtcccgtga tcaaaaccac gccaagtgtt gtgatcaaaa gcgtaacgag aagtttcgac
     2101 acataataag tcattcgatt ccgtcctaaa gataacgtat tgcgaatcgt accgttttga
     2161 aattcttccc caatataaaa gcacgcaaat gctgcgaaag ccagaagaat ccatgcagcc
     2221 attttttcta ccattaaaat ccctgttgca ccgttgatga ctgtatccgt tgcttggttt
     2281 aattcattgc cgtattttgt atgcatatac cattcgttcc aagaataaaa aagcggaagt
     2341 acgaccatca gtcctagtga gacccacatt ttacggctct tccatacttt tattttttca
     2401 gcagttaata gattaaacat gtggattgcc tccagttaat tgtaagaaat aatcttccag
     2461 tttttgccca actaggtgaa taccatccac aggaactttt gcttctacca atgcttgatt
     2521 gatggctgct acttggtcaa ttccttcaaa aagctggata acacggctag attgtacaaa
     2581 ataatcccta taaccaagag agtcgagaac cgttactgct tgttccgtcg ccgaagtgtc
     2641 aatttttata aattgacgag attcttgtgc caattcttct ttcgataatt ctttgatcaa
     2701 gtgtccttca tgcaaaatac catagtgagt ggcgatctgt gacaattcat ctagcaagtg
     2761 gctagaaagc aagacggtga tgccccgttc ttttaccaaa cgttggatga tttctcgcat
     2821 ttctgccatc cctgctggat ccatcccatt cgtcggttca tccaaaatca aaaattctgg
     2881 attcgtgatc agtgcatttg caatagctaa acgctggcgc atacctaaag agaaattctt
     2941 tgctttcttt ttccctgtat tttccaatcg catcattttc agcaggtctt cgatttctct
     3001 atccgacaca ccgccgttcg ctgcttggat acgtaggtta tctcttgcag ttaattctgg
     3061 ataaagagca ggggtttcaa tcgattgacc catacgtcgt cttgctgcct gcaaatccgt
     3121 cgttccaaat aattcgatcg aaccttcatc aatcgagatc aaacccataa tagttctcat
     3181 aaaggtagac ttacccgctc cattttcccc aatcaatcca tagatcattc cacgtttgat
     3241 ttcgattgat actttatcta atgctttggc agcaccgtat tttttggtta taccagttgc
     3301 tttcaagacc gtttcatttc caaaatccat acttatcctt ctttcttatt gatgagtgaa
     3361 gtatagttga cgggtataaa gaaagcagcc agaaatgtat aaagaaagtg taaattttcc
     3421 aattgaatgt caaaaaaaac aatcggacaa tacgtgtctt tcctattatc aggcagtttg
     3481 ttatacgctt gtatcagttt cctgattctt tcttttcgac caaagtcgtt tctctgtatt
     3541 cctcttcact gaaaattttt gcttctttca gatcaagctt gagtaatttt accagaccgg
     3601 ttataaggat aactatagca agactccctc ccagaaaaat tagactgtct cgcagacgtg
     3661 gttgcgttgt cttccaatca aagagcgcac caaacagtgc taatccaagt ggactggcca
     3721 ccatggtaat cgttgttatc gttgacataa gtcgtccttg tatatgcgtt ggaacttctc
     3781 gctgaagcca gaccatataa aaagcaaaag aaaacattaa tccttaaaaa cggacttatg
     3841 aaaaataata catgccgttt gggatttaca attagctacc aaataagagg ttatgacaaa
     3901 agacttttca caatcccttc ttccgaataa acggtgttct cctgccaact cctcggtcac
     3961 aagtcacaat atttatcaaa catgagaagc aatttttgta aattccttct tatttcttga
     4021 agctgaccac ttctgtcaca acctcagaag cggataaata atattgtttg atgatacttt
     4081 atctttttcc cgtttgttta tagatcgaat gatattatag accaagaatc ctacctatta
     4141 acatagctaa aaacacaaaa aaggcacttt ccaaattgtg gaaagtgcca gaaaagttag
     4201 gattaaggta actgcgctca gcattgcttc gcccagtacg attctcgtaa ctcgtgaact
     4261 cgaacgataa tcgcaattaa cgttttgaga actgtgaagc tttacgggct ttcttaagac
     4321 ctggtttttt cttcgtatca ttgaataagt
//
LOCUS       DACCXM010000163         4337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCXM010000163 DACCXM010000000
VERSION     DACCXM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.172"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            193..762
                     /locus_tag="IVY61_002621"
     CDS             193..762
                     /locus_tag="IVY61_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7971680.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(940..1380)
                     /locus_tag="IVY61_002622"
     CDS             complement(940..1380)
                     /locus_tag="IVY61_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP7971681.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1538..2302
                     /gene="map"
                     /locus_tag="IVY61_002623"
     CDS             1538..2302
                     /gene="map"
                     /locus_tag="IVY61_002623"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP7971682.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2334..3257
                     /locus_tag="IVY61_002624"
     CDS             2334..3257
                     /locus_tag="IVY61_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP7971683.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(3482..3721)
                     /locus_tag="IVY61_002625"
     CDS             complement(3482..3721)
                     /locus_tag="IVY61_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7971684.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(3726..4145)
                     /locus_tag="IVY61_002626"
     CDS             complement(3726..4145)
                     /locus_tag="IVY61_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7971685.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
BASE COUNT         1393 a          769 c          794 g         1381 t
ORIGIN      
        1 ttcaaacaat gaggctttaa ataaaaaaag gaggttattt ttttaaggtt aagtttcatg
       61 atatatgtta ctacttgcta tttcagcttg tgaagaaaat caaaaactaa ttttgcatat
      121 caaaaaaaac atgttacaat gaaattaatc aatggtggaa gcgttataaa aaaattaagg
      181 agattaaaaa atatggcaag aaaaaagacg attactagag aacagatttt ggcagctgct
      241 tatgaagtag ctgccaccga tggatttaca cgatttactg cgagaaacat cgcgaacaaa
      301 atgaattgtt ccacgcaacc gatttatctt gagtttaaaa atatggatga cttgagacaa
      361 gctctttttg agcagatcta tgatcattta caatatgaag tgttcactgt tgagcataca
      421 gggaatacga ttatcgatct tgcttttaat tatatccatt ttgcaagaag agagaagaag
      481 ttgtatcgtt cactgtactt agaagaatat ggtggaggaa aagagatgca gaatttctct
      541 tatacttact ttgtgaatac agtgaaacgc gatccagctt atgtgaatct ttcagaagag
      601 cagatcgaat ctttgtataa tgggacatgg atcatagcta ctgggatcgc agctttgatg
      661 tcttcaagca tcatccatcc aactgatgaa cagattgaaa agatgatcca agacagtata
      721 gatgctatct tgaaattcaa agaaccaatc gaaatcgatt gaatatactc aaaagttcca
      781 caaatcagta ggtaacaaga gatgtgtcaa aataaggaaa agttcgtttt tcttacagcg
      841 tatgcatttt taactcccga agaactcttc catgaatgca atgaataaaa gagccataca
      901 gattgaataa atctggatgg ctcttttatt gcaagtatat taaatttttg cgactagttc
      961 tttagcgaaa gcttctaaat tttcgatatc ttcttcttca gcattcagat ccactttaac
     1021 attttcggct ccttttgatg cgcctgtttt tgcaaaagca gcatcgaaat catccaccga
     1081 tttgcagaat tcatcgtaaa aagtatcacc agatccgcag acaccgtaaa tttttcccaa
     1141 tagatccaat tcttgcaagt cttcataaaa gtctacgatc tcatcaggca gatcgccatc
     1201 atcgtatgta taagtagcta ctacgcaaat atctgcctcc tcgaattcat ctgcatctac
     1261 ttgggtacat tcgttgattt cgacttcgat atccagattt tcaaatgctt ctgcaacaat
     1321 atcggcgatt tcttcagtgt ttcctgtcat gctagcatat acgatttttg ctaaagccat
     1381 gatgtgcctc ctcgtttata attatttaac aattatgaat tataccaaaa catcattgaa
     1441 aagaaaataa aacctagaga cacctgggga atgtcatttg gctaaatgta tgccgctatg
     1501 ttaaaatgag agggatttgt aaaaaggaga cgaaaatatg attactttaa aatcagcacg
     1561 tgaaattgaa atgatggcag aatctggagc tttattagca gatgtacata aaaatctgcg
     1621 tgattttata aaaccaggaa tcacaagttg ggatattgag gtatttgtca gaaactatat
     1681 cgagagtcat ggcggaattg ctgcgcaaat tggctttgaa gggtatgaat atgcgacttg
     1741 tatcagtata aatgatgaaa tttgtcatgg attccctcgt aaaaagcctt tgaaaaacgg
     1801 tgatttggta aaagtcgata tgtgtatcga tttgaaaggc ggcatttctg attcttgttg
     1861 ggcctatgta gttggagaat ctacacctga gatcgaccga ttgatggaag taacgaagaa
     1921 agctttgtac atcggtattg agcaagcaca agtagggaat cggattggtg acatcggtca
     1981 tgctatccaa acttatgtag aaggcgaaaa tctagcgatc gttcgtgatt ttattggtca
     2041 tggcgttggt cctacgattc atgaagaacc agcagttcct cattacggag aagctggaaa
     2101 agggttgcgg ttgaaagaag gcatggttat cacgatcgaa cctatggtta ataccggaac
     2161 ttggaaaatg aaaatggatc ctaacggctg gactgcgtat acaagagacg gcggactttc
     2221 ttgccaatat gaacatacat tggctattac taaagatggt ccaagaattt tgacatcaca
     2281 aggagaagaa ggcacttatt gatctatcca gtcaataggt acgggagagg tacatgaaag
     2341 tttggaacaa aataaaacaa aatgaaaatc tgatgcgctt tattgagacg actcaaaagc
     2401 gcatcactga ttcagaaatg agtacgacct ctgtcgtcgt tgcttattat ttactcttgt
     2461 ctttatttcc attgattatt gcttttggta atattttacc gtttctccac atcgatcaag
     2521 agactgtact gacctattta cgagaagtga ttcctgagac cgtttaccag tttatcggtc
     2581 ctgcaatcaa agaccttttg acacaaagtt caggaggctt gttatccgtt tctgcattag
     2641 cggctttatg gtctgcaagt caaagtatca atgctttgca aattgcgatg aataaagcgt
     2701 atggcgtaga aaatagaaaa aattttttga tcgtccgttt cttctcacta attgtcattg
     2761 tcttgttcat gattgctatt agtggggtaa ctatcgttct tgggttggga cagctgattt
     2821 tggatgctat tcagccaatc gtacaaatac ccgtgaactt tatcgatcag ttccaaactt
     2881 tgaagtggcc aatcacattg gtagctttat ttgtcatcat gttcttgatt tatttaattg
     2941 tccctaatgc tcagttgaag ttgaagacga tcattccggg cacgatcttc gctactgtcg
     3001 gctggatgct gttatcacaa gtgtttagta tctatgctag gtattttgca acgaggatca
     3061 gcggttatca gatcatagga agttttattg tactcatgct ttggcttaat tttgctgcta
     3121 cgatcatcat tttgggcgga atcatcaatg cagttgttca agaatacgtg acgggtcatc
     3181 aaatcaaaaa acgtcgagat ccaagccgaa aatggttcaa taaattgaaa actaaatttt
     3241 ctcataaaaa aaagtagaaa caagagttct tattgtaaat ttatatcagt aatttatttg
     3301 aaattttata tctaattttc aattatccgc ataaacacta tacattcaag cattttgaag
     3361 tatagaaaaa cactcatctt accacgaatt taccacgatt gaatgagcct tgatatgacc
     3421 gtaaaactac taagggagat agcacgaaac tgtgtatctc ccgatttttt tatacagttg
     3481 attacttcac tccgatttca aatttcaata ttgctttcat catgtctgta tgaatttctc
     3541 cttttagttc ttcatcaacc tgcataatga ttttaccact ttcagagtgg taaaaaggac
     3601 gcaagcataa tttagaaata taaccgtcat aaaacttcaa aatctcttta actgccattt
     3661 catctccgct tgacgctttg caaatcaatt caaaagaggg atagccataa tctttactca
     3721 tttctttact tcctttcgct caaaagcatt tttaacttcc ttagtcctct agttcttcta
     3781 ctgcaaatag cagaacgtga tacatggtac agttctgaaa tttctctgtc gttcattcct
     3841 tgaaaataac gtaataaaat cgcactacgc tgtttttctg acagtctatg taaaactgta
     3901 ccaagtgttt catcagatac ataaattgta aagtgcaaga ctttaaatga tacacaatca
     3961 taaaaacagg tatcaatcgt tccacgttca acttgtttcg tttcggctat ttcacaaaac
     4021 aatacttcac gtttagaatg tcttgcaagc tgtctgtttc gacttttcac tttgttctta
     4081 atgacaatca gcattaaaga attgaactgt aatctgacga tatgctcgaa agaagatgtc
     4141 ctcataatct cacccccttt ctgctaatgc tgaaatatat aaacggaata ccgtttatac
     4201 cctttagaaa tacagaaaaa tggtaagaaa aaagcccaca caaaacatat atttgtgcga
     4261 gccttaaata ctgcaatatt actctttctt ttataacact atccttttag cagatttcaa
     4321 attttctttt atatatt
//
LOCUS       DACCXM010000164         4323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCXM010000164 DACCXM010000000
VERSION     DACCXM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.173"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(176..1462)
                     /locus_tag="IVY61_002627"
     CDS             complement(176..1462)
                     /locus_tag="IVY61_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7971686.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IVY61_002628"
     CDS             complement(1455..2723)
                     /locus_tag="IVY61_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7971687.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IVY61_002629"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IVY61_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP7971688.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IVY61_002630"
     CDS             complement(3495..3941)
                     /locus_tag="IVY61_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7971689.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="IVY61_002631"
     CDS             complement(4034..4279)
                     /locus_tag="IVY61_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971690.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         1226 a          884 c          757 g         1456 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tctccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 att
//
LOCUS       DACCXM010000165         4283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCXM010000165 DACCXM010000000
VERSION     DACCXM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.174"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            75..503
                     /locus_tag="IVY61_002632"
     CDS             75..503
                     /locus_tag="IVY61_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7971691.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="IVY61_002633"
     CDS             490..732
                     /locus_tag="IVY61_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7971692.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="IVY61_002634"
     CDS             complement(1032..1247)
                     /locus_tag="IVY61_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971693.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="IVY61_002635"
     CDS             1354..1668
                     /locus_tag="IVY61_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7971694.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="IVY61_002636"
     CDS             1772..2950
                     /locus_tag="IVY61_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7971695.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="IVY61_002637"
     CDS             3353..3646
                     /locus_tag="IVY61_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP7971696.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1466 a          646 c          823 g         1348 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt
     4261 aagcaatact tcaattaatc aag
//
LOCUS       DACCXM010000166         4129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCXM010000166 DACCXM010000000
VERSION     DACCXM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.175"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            56..1357
                     /locus_tag="IVY61_002638"
     CDS             56..1357
                     /locus_tag="IVY61_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7971697.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1384..1644
                     /locus_tag="IVY61_002639"
     CDS             1384..1644
                     /locus_tag="IVY61_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7971698.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2643..3878)
                     /locus_tag="IVY61_002640"
     CDS             complement(2643..3878)
                     /locus_tag="IVY61_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP7971699.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1180 a          667 c          864 g         1418 t
ORIGIN      
        1 ctttgcgttg tcatgcaatg aatggttcat attttattga aaaggagtgg ttttagtgga
       61 aaagctgatg aaatgggtcg aagagaaact tgtcccacca atggcaaaaa ttggtacaca
      121 gcgtcattta ttagcaattc gtaatggggt tgtttcaaca ttgtcattga ttttgattgg
      181 gacatttttt atggtcttta tcaatttacc gtttcctgga tggaatgaat ttattgcccc
      241 ttattcggca acgattgttt taccctttag gatgacgatg ggattgatgg ctatttatgc
      301 aacttttgtt atgggttcgg atctagctaa atcatatgga ttagatagtg tgacaggtgg
      361 tatattatct ttaggaacgt tctttatgtt acaagttcct gtaaacgttt taactcctga
      421 agaggcacct cttggctggg tcttacctat gagttctttg ggagcatctg ggatgtttgc
      481 ggggatctta tctatgattt ttgcagtgga agtatatcgt ttctttaaaa atcgaaatat
      541 tactatcaaa atgccagaac aagtgccacc tgctgttgcc cgtagttttg aggcattgat
      601 tccaggtgca gtcattttaa caattacttg gctgattcga agcgtcgcgg gctttgatgt
      661 aaacgctgcc ttattatcgt tattcgagcc tttaaccaat attttaggga ataatttgct
      721 tggtgtttta ctaccaatgt ttttgatcca tttgttgtgg tcatttggta tccatggcat
      781 gagtattgta ggggctgttg ttcgtccaat gtggctgatc atgttggatg agaatgcaaa
      841 ggcattggca gatggtacag ctgccaccca attaccctat atcgctcctg aacagtttta
      901 tcaatggacg gtaacaattg gtggtgcagg agcaacgatc gtggtatctg ttttgtttat
      961 attcttctgt aaatcaaaat tcttgaaaga ggttggtcgt ttttctatta ttcctggtat
     1021 cttcaatatc aatgagccca taatttttgg agctcctatg atgttgaatc cttacatgtt
     1081 catcccgttc aacttagttc ctttagtttt gactattatc agttattttg cagtcaaatt
     1141 agaaatggtc aatagattta ctgttttgcc aacatggaca ttaccagctc caatcggcgg
     1201 ttatctttca gcaggaaatg actggcgtgt tgttatcttg atcatgatca acacattgat
     1261 agcatttatt atctattatc catttgtaaa agcttatgat aaaaagatgc tgtcagatga
     1321 acaaagtcgt ggagaaaacg aagtccagac tgtctaagtt tggtttttag aaggtgggaa
     1381 acaatgtatt ggctgatttt attttttgtt tttatctttt tattaacggc tagtcacctt
     1441 atattgaata tgctagctac atatcatatt caaatcaatc gatggatttg ggcactggca
     1501 tcatttttga tcgtgattct tccaaagatc attgtaccgc atatgaatgt tttgttttca
     1561 tgggggacat atgtactttg tggaattttt gctatcaatt ttatgatcga gcagcataga
     1621 tggtttgtca catcgaagct ttaaatgttc taaaagtggt tgtaacagaa gtggttagct
     1681 tcaagaaata agaagaaatt tacgaaaatt gcttcttaaa tttttgtgaa atgttggctt
     1741 atttttcgtt tggttgaact tattaccgca gatgcaagct tttgaccacc gtttattcgg
     1801 aatatgggac tgtgacaagg cttttgtcac agcccttttt tacataaaga ttattattgt
     1861 tttaactgtt ttggcagaaa gaaggaggaa aaaatggaaa ttttatcagc aatggattta
     1921 gaattagtag atctactacc aaagtgatca agtcaaatat agatttggga gcttacaacc
     1981 atatagtagg ggctgcagta tggtgtatga atggtaatat gtatgatgtg agtgtttatt
     2041 caaatcatga aacgtgtata gaaataattg tgctgggaat agcaatcagt catggtgaac
     2101 gtgactttta gtgtatcgtt gctgacgaca tagaacattc tgacatgatc tactcaatat
     2161 gtggggattg ctggcagttt atataggagt atgcatcgga ttgcgagatc attgtctcaa
     2221 cagaagaggg aggttgatta tcaaagataa agtggtaaat cctacatttg atttagtaat
     2281 tgttgagggg actagcggca caaatctcta caaagcgatg cttctagatc tatatgacac
     2341 attttaagaa aatgtatttg cctaattatt atggtctttc ttttgaagaa acgttgagat
     2401 gatccaacca gagaaaggtg tcaaagaatt tgactaagaa taaatggcag acgctaccta
     2461 tgctcatttg gatagcgtca gatcttttat tttaaggatg attttgtgtt ttttagacac
     2521 cgtaatttaa gtaactctaa cttttcaatc cactatttac tttttttgta ttttcactgt
     2581 ctgggtctcg aatagcgaaa ttactgatga gtctctatta aaaaagcttt ttctacttga
     2641 aactatattg ggtggttgct caggaaggtt tcgatttctt gaattgcttg ttgtggacca
     2701 tttattggaa aaacggcttg cagcgtgcgt gcctgctgac gaatcttggg tgaagaaact
     2761 tcgcgcagag caatctgcag ttttctttca gtcactggtt ttgtgagggc gattgcgttt
     2821 ccgtatttta cacaatagtc aatattacag ctttgctcaa attgcatccc cacaccgata
     2881 aacggtttgc cggattcaca ggcagtttgg acggtccctt ccccgccgtg gataaaagaa
     2941 aaatcaatga attcatgcaa aagatgacta gacagatatt ctgttattaa aagatcttct
     3001 gagtcagatt gaatgtcatc aagcagatag tcaataggtg caataaccgc tagatccggt
     3061 tgtttttgta agaagaacaa aattttgcgt aacatacgcg gattgcctga actacccatt
     3121 gcgaaataga taatacgttt tctttcttgt tgctttcgct gtataaaagt tagcacttct
     3181 tcaggcaagg aagtactgag cttagcaaaa attggaccaa cgattcgata attttccggc
     3241 agctgttctt cttccaaaaa tagtttaggc gttgtgatga gatttagatc agcatccatc
     3301 atttgtaaac ttgtttttgg taaaggaagc atttcttttt gtgcagcgtg tttgaaggca
     3361 ttggggatcc atcgggtatt tactatacgc ttttttgtcc agtgccaaag gggattgctt
     3421 aaagggccga agaaacgtaa ctcgtggggc atcagcgcat gctctgattg aaaaaaaggt
     3481 tcactcaaag catagggttt aacatatact aaaggaatct ttttattgcg ggccgataaa
     3541 aaaattgtca cattcgaacc tgtcacaatc agtcgaggct caaacttagc aatcagactc
     3601 tgttcactag tgatacgaga ggcaaccatt tctttggtaa aagggttttt aagtgtacgc
     3661 aactggtcaa attttatgat ttgttcttct tgttttttcg taaggatcgg cgctagtaaa
     3721 tgatactcaa agccactttc tgtgatcaaa ggagcaaaag ttttagaaaa gccaaagaat
     3781 acacagtaat aacggtcttt catagctttg gcaacctcaa tcattcgtgt tgtctcagct
     3841 aaattgaata ctgcaggagc aaaaagaatt gtcttcatga aatcctcctt attggaatga
     3901 gtctatccct tcgtttctta ctagtatact aaaaaacaca gcgttcgagt agcctatgta
     3961 tattttgaat attaaactgt ctctcgctgt tttcacaact tctaaattta aattttgtaa
     4021 tcaagttgaa actccaattt tcaaaaaaac acgcaacaat tttttgatcg atttatatgt
     4081 tgtgccagat aaatacgaga agttgtgtca ttaaaaataa acatgagaa
//
LOCUS       DACCXM010000167         4103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCXM010000167 DACCXM010000000
VERSION     DACCXM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.176"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            123..902
                     /locus_tag="IVY61_002641"
     CDS             123..902
                     /locus_tag="IVY61_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP7971700.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            895..1212
                     /locus_tag="IVY61_002642"
     CDS             895..1212
                     /locus_tag="IVY61_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7971701.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            1456..3066
                     /locus_tag="IVY61_002643"
     CDS             1456..3066
                     /locus_tag="IVY61_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7971702.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            3223..4071
                     /locus_tag="IVY61_002644"
     CDS             3223..4071
                     /locus_tag="IVY61_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7971703.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         1204 a          711 c          835 g         1353 t
ORIGIN      
        1 catgaattga agcaaaaatg agaaaaatat aatcttgttt ttaaataaaa cggctacaat
       61 ttagcgaatt tatgttacaa tgagtccgct gtttatttgt tgtaattaaa ggagatattt
      121 taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt gcatgcagtg atgccacttt
      181 gtataagcta tataccagta ggattagctt gcggagtatt gttgcaaaaa gttggatttg
      241 atcctctctt atcaggactg ttatcgattc tggtcttttc cggtggcgcg cagtttttag
      301 ttgcatctat gcttacgacg caagcatcat ttgcaacgac attattgatg gttttctttt
      361 tagaattacg ctatacatta ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc
      421 ggacgttttt ggctgtcttt tctcaatcac tgaatgatga aaactatgcg gtgaactact
      481 taaagttttc aactgaccct tcctggaata aaagaaaggc tttatatgtc aattggttct
      541 caatgactgc atgggccggt agcaatatga tcggtaattt tttcggctcg gtgattcgag
      601 tagatgctga ccttgtccat tttgctttga cggccatgtt cattttcatg tttatcatgc
      661 agatgaaaag cgcactactg atctttacag gtttattctc aggcgtatta ggggtcgtat
      721 tcatggtatt attccaaaat actttcggat tgattgcagc aacagtcatt gcttcatttg
      781 ccggatttat gctggagaag gcattcaaaa aagaaaaagc taagaaacta agagaccgcg
      841 gtaaagatgt caatgagccg ctcttcaact tcccggaaca ggaggccaat caccatgact
      901 aaactatatt tgctactttt agttgcttgt ctgttcatcg tggcatatat cccgcgcttg
      961 ttcccgatgc tttattttac tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa
     1021 tatgtaccgg tggcattatt tgcggcgtta gcttttaaag atgttttcat tactcatgaa
     1081 catctggata ttgcttggaa tatcaaaata gctgcaatga ttctagtagc aggcgtggct
     1141 tacaaaacac gttcgatggc gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg
     1201 tctatgcttt aaactgtttt attttattaa aaacagagat tgcgacataa gttgttcaaa
     1261 ctgatctatc cgaactgcat ttgcaacagt caattctgga tgcaagttta ttcgtaggga
     1321 taaaaaagat tagtgttggc ttatgatgca agctcttttt tattttagga attagagagg
     1381 gatttgtcct tctatgattt tttgctataa tagggaagat gtttatttta tgaaagaata
     1441 gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa cattttggct aacctttgct
     1501 aatcttctat gtaaagtttt aggggtagtc tatttgattc cctggctaag tatgatgggg
     1561 aacaatcaag atggtatgct tgctacgaca ttgtataatg ttggctatct tccttatgga
     1621 ttgttcctga tgcttggtac agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta
     1681 gccacaaaag aaggagataa cgctgcttgt cggctgattt ttagaagtac gatcaatatc
     1741 atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt tatttgctcc tttactagcc
     1801 ggaatcagtc cgattgctaa cgttaataat gggatactag ctatcagaag tttatgccca
     1861 tctttgattg ctatcccaat tcttagtgca atgagaggat atttccaagg aaaaaatgat
     1921 ctgcggcctt atggaacttc tttgattatc gaacaggctg tgcgagtgat cgttatcttg
     1981 gctggtactt attacttgcg cgtattgact gacggaacca tattagaagc tgtattgatc
     2041 agtacagtcg cttctttctt cggtgggtta gctgcgatta tccatatgta tatcgtcgga
     2101 cgcagaaaag acttcttcga acttaaagat tttttgattt ccagccgtta tttcgaaaga
     2161 gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac taccgtttat ttttgtcggc
     2221 tcagtcatta ctatcgttca aatgatcgac caagtaacga tgaaaccact gcttcacttt
     2281 ttccgtccag agatcgcaga tcagcaactg gaattcttat tcagccgtgc ttctgtcaat
     2341 ccaaataaat tgacattgat cctgatctcc atggtaggaa cagtagctat aagcagcttg
     2401 ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat
     2461 agtttttcga ttgcgttatt gattcttctt ccttcattga ctggaatgtc tttattagct
     2521 ggaccactat acacattgtt ttttggctat gatcctgaaa gtgtcggata tttccaaatg
     2581 gctttgctag cttctttgtt tttctcttta ttcacgatcc tatctacgat gcttcaatct
     2641 ttgaatcacc atcgttttgc gatccgatta actgtagaag caattatcct gaaagtcatc
     2701 ttccaagtaa tcggattagg attagttggc ggatacggga tgagcttatc aaatggctta
     2761 gcattcggta ttgtctttgt tcgcggctat cactttatgt gcaaagaata ccgaatagca
     2821 cctttagcaa agatcagcaa ttttttctta aaaactttcc gctccaccct gattatgctt
     2881 gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc
     2941 tatgcaatga tttattgtgt ggctgtagga agtattggcg gcttagtatt tttattttct
     3001 caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa
     3061 gaataggtta actaatcgtt ttttgctgtc acttgaaaaa cggcaactcg tatgattacg
     3121 cttgtcgttt ttctattttc caattttagt ttcatatcta atcttactta tgatatagtt
     3181 ataaggtctg gaaaaattct ttgaaacgag gaaattaaac acatgaaact tgcagtagtt
     3241 acagatagtt cggcatatct gccggaacgt atcaagaacc atcccgactt attcgttatt
     3301 ccaatccctt tgattatgga tgggaaaatt tataacgaag gaatcgatat cgaagcggat
     3361 gaatattatg gacttttaaa aaacagtaaa gattttccta ctacttcaca acctgtcgta
     3421 ggggaagttt tgggtttata tgaggaattg aaagagaaag ggtatgacac gattttaagt
     3481 atccatcttt cttcagggat ttctggcttt gtcaacacat tatttgcgat gaaagatgac
     3541 atcagcggag taacggtcat tccttatgac tctaagatca ctagtatgcc gatgggacac
     3601 atggtagaag ctgccttaga cttaaatgat gaaggtcagt cccttgatga aatacttgca
     3661 catatcgatc gaatccgaga taatacctat gcttatctga ttgtagatga tttgaacaat
     3721 ttagtacgag gaggacgttt gacaaatggt gcagcgctga ttggcagctt attaaaaatc
     3781 aaaccgatcc tgacattttc tgaagggaaa attgttttat tcgaaaaaat ccgttcaagt
     3841 aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa gaaataacga aatacaaccg
     3901 ccagtcaaac tttacgtgat acatgcaaat aatttagaag tagcgattgc ggagaaagag
     3961 aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg gtcatttcgg accagtcatt
     4021 ggtacacatt taggtgaaaa agccatcggc attgccattt cagcgcaata attcctttta
     4081 ttcaaaaaac ataccaaaaa tat
//
LOCUS       DACCXM010000168         4085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCXM010000168 DACCXM010000000
VERSION     DACCXM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.177"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(123..581)
                     /locus_tag="IVY61_002645"
     CDS             complement(123..581)
                     /locus_tag="IVY61_002645"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP7971704.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(752..1897)
                     /locus_tag="IVY61_002646"
     CDS             complement(752..1897)
                     /locus_tag="IVY61_002646"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP7971705.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1910..2827)
                     /locus_tag="IVY61_002647"
     CDS             complement(1910..2827)
                     /locus_tag="IVY61_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP7971706.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3255..3818
                     /gene="efp"
                     /locus_tag="IVY61_002648"
     CDS             3255..3818
                     /gene="efp"
                     /locus_tag="IVY61_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP7971707.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1280 a          766 c          776 g         1263 t
ORIGIN      
        1 gcgctaaact gaaaactagc tatttgtttt cccgtttaga actcttttca gacaataaat
       61 atttaataaa atacgaagat aagatagcta tattgcttct catctttacg ttcttttaga
      121 aactatgctt ctacttcaga ccattcatcg cgttttgcta aaaattcacg agccaaagct
      181 tgtgcacctt ctagtgtatg gctggcagcc cagccacatt gtgtttcatt gcttgcaggt
      241 acttcagtag cttcaagaac atctttcatt gttgcttcaa gaacatctaa aatgtcttcg
      301 tagctttcat gattcaatac agtcaagtaa aagcctgttt ggcatcccat tggagaccag
      361 tctacgatgt aatctgcgtg attgcggatt agttcggcag tcaagtgctc aagtgaatgt
      421 agacttggca tgtccatgtg ttctttattt ggttgtttaa agcggacgtc gtatttaatg
      481 atgacatcgc catgcgcacc cgtttttcga tcagctacac gtacataagg tgctttgact
      541 tttgtatggt caagattaaa actttcgaca ttcattctca tttgtaagac tcctatctgt
      601 ttgttcattc gttttgatgc ttttcactaa agaattattt agtgaaattt acaaaaacgt
      661 ctttctactt gattctaaca cttatcgttt ccaaggtgta gcaatctgaa atatttttta
      721 gatttttctg ctaagtcgtt tattgggact tctagttctt gattctgtca aatgcctgag
      781 ctaaatcgtt tatgagatcc tgaccatact caattcctac agaaagacgg atcaagccgt
      841 cctttatgcc agcctcttct cgtttttctt ttggaataga cgcatgagtc atgacagaag
      901 gaatttccac taaactttct acgccaccta aactttctgc taaagtaaat agttggagtg
      961 attcaacaaa tggaatagca gattcttcat tttttaaagt aaaagaaatc attccgctaa
     1021 agccgctcat ctgctgctta gcaactccat ggagttcatg atcgggtaat cctggatagt
     1081 agaccttttc tactgctgga tgagaaaata gaaaatcagc taccacaaaa gcattttttt
     1141 gatgttcctc catacgaaca cttagtgtct tgattccgcg ctgaagtaac cagctatctt
     1201 ggggaccaag tacggctccg atagcatttt ggtaaaatcc gatctgatcg gctagatctt
     1261 tatggttaga agtcactaaa ccagctacta catcgctgtg tccgcctaga tatttggtac
     1321 cgctatggac aacgatatca gaaccgagat ctaaaggtct ttgaaaataa ggagtagcaa
     1381 atgtattgtc agcaataact atcaatccat gttgatgagc cagagtggca cttttttcta
     1441 aatcagtaat ctttagaagc ggattgctag gtgtttcaag ataaagtgct tttgtattgg
     1501 gtttgatgga ttgttctatt ttatcaagat tactcgtatc tatgatcgtg tactcgagac
     1561 catttttagt aagaactttg tcaaatagac ggaatgttcc tccatagaca tcatctccta
     1621 ataagatatg atcgccagct tgaaaaagag aaaaaacggc atgaatccca gaaagaccag
     1681 aagaaaaagc gaatcctcga acaccgcctt ccaaatcagc aatcagttct tccaatgcaa
     1741 aacgagtagg attaccagat cttgaatatt catattgttt aggctggccg acgccatctt
     1801 gccgataagt ggatgtttga taaataggta cactgacagc tcctgttgtg ggatcttcac
     1861 tgatgcctcc gtggatcaat tttgtttgaa tgtgcatgtt tctttcctcc tataagtaaa
     1921 tgttttttga taaatagcgg tctgctgcgt ctggaaaaat cgtaacgaca cgatgaccag
     1981 gtggtaaacg acgaatttct cttaatgctg ctgcaaaagc cgcaccactc gaacttccaa
     2041 ctaatagtcc ttgttctcgt gctaactgcc tagtatagtt aaacccttct acatcagaga
     2101 ttgtctcgat ctgattgatt gaaaggggag aaagaaacgg cggaatgaat tctacaccga
     2161 taccctcaat ttcgtgaggg gctggatcac caccatttaa cacagagccc tctggttcta
     2221 caccgattat tcgaatgtcg ggatattttt ctttcaaaaa ggtagaagtt ccagcaaagg
     2281 ttccaccact cccaattcct gctacaaaac tatggatatt ttctttgatt tcatgaaaga
     2341 tttctggccc taacgtatga tagtaagcag caggattatc tttattttca aattgaagag
     2401 gcagatagct gtttgatatc tcctctgcaa gttttttact tttgctgatg gcaccgatga
     2461 ttccttgctc actaggagaa tggatgatct ttgctcccaa tgctttcatc aattgttgtt
     2521 tttctaagct aaacttctct ggaacaacaa aaaccgttct taatttgtat gtgagtgcgg
     2581 ccagagcgat cccaattcct gtatttccgg cagttggttc gatgatcgtc gttttttcat
     2641 tgatttttcc ttgggcaaat ccttctttga tcaaataact gcccaatcta tctttgatac
     2701 ttcctccagg atttaaatat tctagttttg cataaatagc agaaccttta ggcatagaat
     2761 agtgctgatt gtcaaagcga taaagcggtg tggaaccaat agcttctgta attgtcgaga
     2821 taatcatgtg ggcctcctaa aaaaagataa taaaaaaagg agctgactat taaatcagct
     2881 ccttgaaaga catagagaaa aattgagtag ataaaaagat aagttacctg gtactcattc
     2941 tatgaagggc aattaataga catgacaaat agtatgctgc ttttatgcac catacaacag
     3001 caacgattca attgtttttg gttgctgact gtcatgtgta tccctccaag taaaattttt
     3061 attaacgata gcatgaaaaa aaataaaagt caaaggcttg aaagaaaaaa atatttcgat
     3121 agcatcgttt acccgtgttt atttttctga caaataggca tgagacctta aaagatagag
     3181 aatactctag ttttctggcc ggttgtctga tacaataaag ttgattatga taaagaaaag
     3241 aaggagcaaa aataatgatc tcagcaagtg atttaagagc cggtatgact ttcgtacaag
     3301 acggcaaact gattaaagtt ctggatgcaa gccaccataa acctggtaaa ggaaatacag
     3361 ttatgcgtat gaaactgaaa gatgtccgtt caggtgcaac atacgacaca acgttcagac
     3421 cagaagaaaa atttgaaaaa gcacatatcg acacaaaacc tgtacaatat ttgtacacga
     3481 tggatgatac agctttcttc atggatttgg aaacatatga gcaatatgaa attcctgtag
     3541 aaacagtaga agaagaaatg aaatatatcc tagaaaacat ggaagtaaaa atccaatttt
     3601 tcggtagcga agtcatcggc gttcaattac caacaactgt cgtattacgt gttgtggaaa
     3661 cacaaccttc catcaaaggt gcaacagtca ctggttcagg taagccagcg acaatggaaa
     3721 ctggcttagt agtcaatgtt cctgacttca tcgaaacaga tgaattgttg gaaatcaata
     3781 cagcagaagg ttcttatgta aaacgtgcgt caaaataatt cgctaacgga ttgataagaa
     3841 tttgaaaaca gagcttgacc tcttgaaagg cagatcatcg gatctgcctt tcaagaggtt
     3901 ttttgtggaa tcagcaaatt gaaaaataaa aaagtatttg attttattta tattttgaaa
     3961 gatgaatggc aaaagctaga gaaagaaaaa actgcactta tttatgaaga aattgaaatg
     4021 attcataaaa ttgaaaatat ccgaactatt ccgaactata ttgaagcgaa aatatttttt
     4081 catca
//
LOCUS       DACCXM010000169         4070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCXM010000169 DACCXM010000000
VERSION     DACCXM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.178"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            86..1435
                     /locus_tag="IVY61_002649"
     CDS             86..1435
                     /locus_tag="IVY61_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7971708.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="IVY61_002650"
     CDS             complement(1563..2153)
                     /locus_tag="IVY61_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7971709.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="IVY61_002651"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="IVY61_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7971710.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1176 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaaaaa
//
LOCUS       DACCXM010000170         4049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCXM010000170 DACCXM010000000
VERSION     DACCXM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.179"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            82..2022
                     /locus_tag="IVY61_002652"
     CDS             82..2022
                     /locus_tag="IVY61_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7971711.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2109..4031
                     /locus_tag="IVY61_002653"
     CDS             2109..4031
                     /locus_tag="IVY61_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP7971712.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
BASE COUNT         1366 a          693 c          849 g         1141 t
ORIGIN      
        1 ttcagttatt acggaatcat tttgtgctta ataaaaagaa accgttttta tatactataa
       61 atgtagaaag gcggtaggaa aatgaaaaaa attattgcaa tcacatcctg tccagttggt
      121 attgcccata catacatggc agcagaaaac ttagagaaag cggggaaagc agttggcgcc
      181 gaggtaaaag tcgaaacaca tggttccatc ggtattgaaa atgaattgac tgcaagagat
      241 atcgaagaag cagctggtgt aatcatcgca gcagatacta aaatagataa atcacgattc
      301 ggcggtaaac ctttgataac cgtaggtgtg caagaaggaa tccaccatgc agatgagttg
      361 gtgagagata ttttagcagg aaaagcgcca gtctataaaa gtacggaggc aattatttct
      421 tccgaaaata aaagtgaatc agaaaattta ggtaaaaaaa tctataagag tttgatgaat
      481 ggcgtttcgt acatggtacc tttcgtagtt acaggagggt tattgatcgc gatttcttta
      541 acattaggag gaacagctac acctgaaggg atcaagatac cagaaggaac gatttgggca
      601 acgatgaata gtatcggaag tatcgctatg gggttgatgg ttccaattct atctgcattt
      661 attgcgcaaa gtattgcaga tcgtccagga cttgttcctg gatttgttgg tgggatgtta
      721 gcggcaaacg gagcattata tggtagtgat gcgaatgcgg gattcttagg cgggatcatc
      781 actggatttt tagcaggatt cattgcttta ggaatcaaga aagtgcgagt accgaaagct
      841 ttacaatcga tcatgccaat tattgttatc ccaattttag gatcattggc agttggtttt
      901 gtgtttatct atgttattgg agaaccagta gctcttctat ttagttcatt gactcacttt
      961 ttagcaggga tgcaaggagg aagtgaaatt gttctagcta tgatcttagg agcgatgatt
     1021 gcttttgata tgggggggcc gttcaataaa gtagcctttt tgttcggatc tggcttgatt
     1081 gcagaaggca attattccat tatggggcca attgctgtag ctatctgtat ccctccatta
     1141 gcattggggc tggcatcatt gattttaaaa accaaattta cgaaaacaga acgagaagct
     1201 ggtaaggcgt ccttagcaat gggactgttt gggattacag aaggcgctat tccgtttgct
     1261 tcaacagatc cactacgggt gattccaagc attgtaatcg gggcaatgac aggaagtgtg
     1321 atcgcgatgc tttcaggagt gacagatcat gtagctcatg gcggtccgat cgtagcggtt
     1381 ttaggagcag tggatcacgt agccatgttt tttgtagcag tgattgctgg tattgctgta
     1441 accgtgctaa tgttaagaat ccttaaaccg acattacaac aaactgcggt tgctgcagca
     1501 aatggtgtga cagtagcaga tctatcagag actgaaacaa gtgatgttta tacgaaaact
     1561 gaaacttttg attctatcac ggatattatg tcagatgaag caatcgtttt aggtgaaaca
     1621 gcaacaacaa aagacggagt aatcgaagat ttagtccaac gtttagaaaa aacagcggcg
     1681 atcacagata aaaccagatt taaagaagtc atctatgata gagaacatga aagtacaacg
     1741 ggaatcggta tggggattgc tattccacat ggaaaatcag cttatgttct taaaccaaca
     1801 gtggcatttg ccagaagtca atctggggta gattggcatt ctttagatgg aaaacctgca
     1861 catatgatct ttatgattgc tgtacctgaa aacagccaag gtgatatgca cttgaaaata
     1921 ctgcaacgtc tatcacgtaa attgatggat gatgacttcc gtcaagcact gatggaagcg
     1981 ccagataaaa caacagttca ccaattatta agcgaaatgt aacataagat gaaaaatcga
     2041 ccgtgatgag acataataga tgtgttatca tgatcgattt tttatttaga agggagtttc
     2101 tgcacattat gaatcagcgt cagcaatctt tgatctatca attaagtgag aacaataatt
     2161 atataacaac aaaaaagcta gcagagcatt tccatgtgtc agaaagaacg attcatagcg
     2221 atttgaatac cattgaaact tggttgagag acaaacatct gcctttaatg tttgaaagaa
     2281 aaaaaggtac aggtatcttg ttgaatggtc tgcctcacga aagggaacaa ctgaaacgag
     2341 cgcttaatga agagactcaa acagaaatgg ataaaaaaca gcttcgtcag ttattgattt
     2401 tccatctgct agtagcaaaa gacggattta gtcttgaaga actttcagaa aaactatatg
     2461 ttggaaaacg tacgattcgt aaagaattga cagagttgca aagctttttt gagtatcacc
     2521 atttacagct agtatccaaa acaaaactag gaacttttct taaaggcgat gaacaagaaa
     2581 aaagacagtt gcttgttaag acattacgaa atatgcagaa agaagatcca caaagtccgt
     2641 cgttgaaaga attcttcgct aaagatacat taaaagttat ccaacataca ttacaagatg
     2701 ttttttatga aaaccattta gaacagccga atggactggc tagtgtggag attcacatct
     2761 actttatgct cgagcgaatg aaacagtacc agaaagtgaa attaagtaaa gacgaaaacg
     2821 aagtcgtgga acacacccaa gcacaaaagt taagtagtca aatattagca aaactagcca
     2881 ccatctatcc aattgaattt tctcctgatg aaatcaatta tctggctttg cgtatcgcca
     2941 atctttttac aaattcacaa gcagccacac gttttcaaaa ggaatctagc gcacttgctg
     3001 atcatttgat cgaacaagtg gaacagtttc taggctattc attaaaagaa gatcagctac
     3061 taaaacaaaa tttaaggtct catttatctt caacttattt tcgactccat tatggtttgc
     3121 agatttctaa tccattgacg aaaaatgtgt ttagtacgta cacacaactt ttcttagttt
     3181 tacagttaat tttagaagat tattttgaaa aggaaaattt ttatgttcca caagaggaga
     3241 cagcttattt aacgatccat ttccaagcag cgatcgagcg acacaaacat cagaaaagta
     3301 gaagatatca aacggtttta gttagtgaat attcaaaagc tatggctact tttctcgaag
     3361 cacggctgaa cagagaactt ccagaattaa cgattatgga tctagttgaa tataagccag
     3421 aattaaagag acaagatttt ccctctgttg attttatctt ggctacgtta ccatttaaac
     3481 atataagcat tccagtaatc gagatctcac caatgatcac agaaactgat ctagcctatt
     3541 tgacaaaata tatgctggag catgtgccta tcaagaagaa aaaaacattt gatctagcaa
     3601 gctttactca tccatttctg atctttcctc aacttgaacg gacagaccca gccgatattt
     3661 taaactttat gggaaacgtc ttagtggaac atcattatgt ggagccggaa ttcatcgatt
     3721 ctgtcttgga aagagacagt cactccagta ctagagtagc accatttgta acaatccctc
     3781 acggtaatcc gctgtatgtg aagcattcga tgatttctat tgctacaatg aaagaaccag
     3841 tcctatggca cggggaacag atacgtatcg tactgctatt agcaataaag aaaaatgatt
     3901 tgaaacatgc agagttcaaa aaaatatttt ctgttattca ctatctagaa aagcagccag
     3961 aacaaatggc tcagttgatg caatccaatc atccgctaga aatcttaacg ttgttatcta
     4021 gttatgaata agtagcgatt aaaaaactg
//
LOCUS       DACCXM010000171         4020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCXM010000171 DACCXM010000000
VERSION     DACCXM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.180"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(130..1635)
                     /locus_tag="IVY61_002654"
     CDS             complement(130..1635)
                     /locus_tag="IVY61_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7971713.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1632..2318)
                     /locus_tag="IVY61_002655"
     CDS             complement(1632..2318)
                     /locus_tag="IVY61_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7971714.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2511..3932)
                     /gene="gndA"
                     /locus_tag="IVY61_002656"
     CDS             complement(2511..3932)
                     /gene="gndA"
                     /locus_tag="IVY61_002656"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP7971715.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1104 a          852 c          691 g         1373 t
ORIGIN      
        1 attaacggat gttattctat ttaattgttc gctagttttt ttataatgac atccctgaat
       61 ttctcacttg cttgtatttt gtaccttatt ctttgttgga tatataaaaa aacaagtaaa
      121 gcacatgctt tacttgtctt ctgcctttgc gataggaata aagattctaa aaattgtccc
      181 ttgccctact acactttctg ctaagatacg tcctttatag ttttcaacca gttgtttagc
      241 gatagacaac cccagaccat ttccaccctt cgttctagct cttgctttat ccactcgata
      301 aaaacgattg aagatcttat taaggtcttc tttgggtatt ccttcgccaa agtcctgaat
      361 cgcgatctca aattcattca gagtggaaga aatagagata tggacttctt ttcgtgtagt
      421 tgaatacttt actgcgttat ccaatatgat aatgattaat tgttcaaagt gattacgata
      481 aatttgcagg gtcacttctt ttgtcaaatc atcatctaaa gtgatcacat agtctggata
      541 taatatcttg aaattattat aaacttgata ggtgacttct tttcctttag ttgtttcatt
      601 cgcataatga acatctactt gttctgctct tgataagtct agcatctctt gaactaagct
      661 tttcatacgg ctgatttctt gcatgctggc gcttaatgat tcttctaata tctcaggatc
      721 atctttcccc caacgattta gcagactaag atgcccttca ataattgcaa caggtgtcct
      781 taactcatga gatacgtctt ccacgaattg ctcttgttgt tcaatataca aacgcatacg
      841 gtcaagcatc tcattgaata tttcagctaa atctgccaat tcatcatttg tgtcgatttc
      901 aggcatgtgg atatctgatt gcggatcttt acgtatagta tccattgtat ctctgagtac
      961 cttgagtggt tttaagaaat aagaagataa aaagaaacct aaaatactac ttaagatcaa
     1021 agaaacaact tctaaaacaa taagagtcaa taatagctta tttcgaatat catagaaaga
     1081 agataattca tagaagcttt gcgcatagcc gatcttttct cttgtacctt tagaatatat
     1141 aggctggata gataaaaagc cagtttttcc ttctagtgta acgatcgttg gaactttacg
     1201 tgtagtctga atcaggtttc gactgttttc atgggtttta aaaatcaagt tctcgtccaa
     1261 atcataaaca taaagatcca ttgatggctg ccctagctca gctataaagg gatcgatctt
     1321 cattaagttt ccttcgacca gtgaagtatg atctacgacc tcatctccgt catttgtcgc
     1381 tttcgtcaga ttacgatacg tatttactaa tgtcagttct tcatccgagt tagccaaacg
     1441 agaagctact tctgaaatcg tacgctctac attgtttctt tcttttgcca caatcagatt
     1501 gatagatgat ttataggtaa tgacagcgaa aattgtaaac acaacgaata tgaagaaaga
     1561 actcgcaaag gcccacttga ttgtcaagga gggccctttc agttctcttt tttccgattt
     1621 tttcggcttt atcacgagcg catcacataa cctgttccgc gtacagtttg gatatagctt
     1681 tcctccccgg gaacgtcaat tttgttgcga agataacgaa tatatacgtc cactacgttt
     1741 gtttcaactt ctgtttcgta tccccacact ttatttagta aaacgtcacg tgctaatacc
     1801 acgttcacgt tttccattaa agtaagcagc aactcgtatt ctctttttgt tagttcaatt
     1861 acttcatttc cgcgacgtac gacacggttt tctttttcga tagtcaaatc gcgataagta
     1921 atagtagttt gtttagccac atttttatcg ccttcaatat cgattcgacg caacaatgca
     1981 cgaagacgtg ctaataattc ttcgatagca aatggtttga cgatgtagtc atctgctccg
     2041 tggtcaagtc cagatacacg atcgatcaca gaatctcttg cagtcatcat aatgattggt
     2101 gtatttttga cttggcgtac acgtctgcag acttctaaac cgttcaattc tgggagcatc
     2161 agatccaaca atatggcatc ccactcatta tttaatgccg catctaaacc agttcggccg
     2221 ttgtagtgga cttctgcgtt atacccttca tgcttcaact ctagctcgac aaatcgtgcc
     2281 aagttctttt catcttcaat gattagaata ttgctcattt attattttat cctttctatt
     2341 ctactggctt tttaaaaact ctatcattat accgataata tgcttaaaaa tctatagtca
     2401 ttttatcatg gatagcaatt ttaaaaaaga gctgaagtct actcataaca atcgacttca
     2461 gctctttcgt cctcttttta ttttctcatt tcctaacagc cgaaagacta ttattcttca
     2521 tggtaccatg agtaatggaa gattccttct ttgtcgacac gctcataggt atgtgcgcca
     2581 aagtaatcgc gttgggcttg gattatattt gcaggcagac gttctgctct gtatgaatcg
     2641 aagtaagcga tggctgatga gaaagtcggt acaggtacgc cagcttggac agccaaggca
     2701 acgacgtctc tgactgattg ctgatatttt ttcgtgattt caacgaagta atcatctagc
     2761 aataaatttt cgatctcagg atttttgtca taggcatccg taattttttg caagaatcga
     2821 gctcggatga tacatccagc gcgccagatt tttgcgattt caccaaatgg caagtcccaa
     2881 tcaaattctt ttgaagcgac acgtaattga gcaaatcctt gggcataact catgatttta
     2941 ctgaaataca acgcttctcg gattttttcg accaattctt ttttgtctcc cgtgaactca
     3001 aaatcgttcg tacgagacaa aatcttgctt gcttgtacgc gttcttcttt ataagcagaa
     3061 atatagcggg caaatacaga ttcagtgatc aatggaagtg gcacacctaa gtcaagcgcg
     3121 ctttggcttg tccatttacc tgttccttta ttcccagctg catcaaggat cacatctact
     3181 actggtttac ctgttccttc atcgtcttta cgtgtcagaa tgtcagcagt gatttctatc
     3241 aaatagctgt ctaattcgcc ttgattccat tctttaaaga tatcagccat ttcttctact
     3301 gaaaggttta aaatattctt cattaagtca taagattcag caatcagttg catatctcca
     3361 tactcaatac cgttgtgtac cattttcaca taatgtccgg ctccatttgg gccaatatat
     3421 gtgacacatg gtgctccgtc ttctgctttt gcagaaattt gctctaagat tggtgcaact
     3481 agcgcatagg cttctttttg tccaccaggc atgattgaag gtccttttaa agctccttct
     3541 tcaccaccgg aaacgcctgt accgataaag ttgatgccag agttggctaa ctcttcatta
     3601 cgacgcatcg tatctttaaa gaatgtattt cctccgtcaa tcaaaatatc gcctttatca
     3661 aggtgaggaa gtaactcttg gattgttgca tctgttgccg gtcctgcttt gaccattagc
     3721 aagatccgac gtggtttctc gatagattct acaaattctt cgattgtata tgttgctttg
     3781 aaatttttat ctgggtgttc ttctactaca gcggttgttt tagcccctgt acgattgaat
     3841 aaagccactg aatagccgcg gctttctatg ttcaaggcta gatttttacc cataacggcc
     3901 ataccgacaa cgccgatttg ttgttttgac atatgaactc ctcctacata cgggatttct
     3961 ccctaatctc ttacattata gcttaacacg gctacattgc aaactaaaga acaacatatt
//
LOCUS       DACCXM010000172         4004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCXM010000172 DACCXM010000000
VERSION     DACCXM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.181"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            456..1949
                     /locus_tag="IVY61_002657"
     CDS             456..1949
                     /locus_tag="IVY61_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7971716.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2084..2380
                     /locus_tag="IVY61_002658"
     CDS             2084..2380
                     /locus_tag="IVY61_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7971717.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2481..3332
                     /locus_tag="IVY61_002659"
     CDS             2481..3332
                     /locus_tag="IVY61_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971718.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3353..3565
                     /locus_tag="IVY61_002660"
     CDS             3353..3565
                     /locus_tag="IVY61_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971719.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1424 a          580 c          814 g         1186 t
ORIGIN      
        1 atattcaaaa tgtcaaaata tatttgtgcg ttttgcgttg acaatttaga gtgaaaagag
       61 gtaaacttaa ttctatagaa accacagata attcactatt tacaaagtag caaaagcagt
      121 ggtactcttt aaacagaaaa attacataaa aaaaagagac ggaaataatt gattttagca
      181 gaatcaatta tttctgaatc acatggttag gacatgtgtt cacgctcgtc tcaagaatat
      241 gtaagtagct accaactact tatattaaaa gttgatcgga taccttttcc gattgccttt
      301 attctaccaa aaaaaggcga gcaactcaac agaagaacgt ttatttagtg tacttaaatt
      361 aaatattctt aaacactgaa gagagaacac atactatggc tgagattaaa gctaaactgt
      421 gttctctttt tgtttgagaa aggacgttac aatcgatgtt gagcgaaatt tattcagcta
      481 ttcttaaaaa cggcttacgt acatacaaat atcgaaacag caccatgaaa cctgcaggtc
      541 atattgattc cagtaggagt ggtgctatct ttgcgtaccg tagcaagcaa ttaatgaatg
      601 ttgggcgtgg tatggtcatt acttcggaag aagctatttt agagaacgaa aagaaactaa
      661 cgcattggac acctaatacc tatcgctttg gtacttatgc ggatgattat cgaacagtgg
      721 ttaaaggtca ttctgagaaa aacttaagtc aaatcaacac atttgtagtt gatatcgatt
      781 caaaagagaa ccatcaaggc gaaattattt tagcctgttt agatcaagtg ggctacatgc
      841 caacgctaat tttagaatca gatcatggtt atcaagttta ttttgtacta aagacaccag
      901 gctatgtcac aaagaaaagc aatttcaagg tgatcgatgt agcaaaaaaa atttcaaaaa
      961 caattcgtct acagttggct gaaaaattgt ccggagtgga tctaggatgt aaccacttcg
     1021 gaattgctcg attcccacaa gaaaataata ttgtttttaa agaaatgaac tatcagtatt
     1081 cttttgctga ctggattcaa tggtcgatga agcttgaatc agatcaaaaa acaaaagaag
     1141 atcgagaacg aaaactaatt gtctttcctg agaaaaaaga aattcgtcaa gtagatgaac
     1201 cgtggttcga tatgttactt cataaagcaa acatcgtcgg cggagaagga cgtcttggtc
     1261 gaaataatgt gattttcacg ctttctttag cttattactc ttcaggttat gcattggata
     1321 cttgcgaata caatatgttc acattcaacg aacggttaca ggaaccgcta tctgaagcag
     1381 aattagtaaa gattgtacgt agtgcttatt ctggtaatta tcaagcagca agcagagaat
     1441 tcattattga attgtgccag caatgggttt caccaacctt aaaaaataat gatctgttca
     1501 ttcaacgacg tggctggtgg aaatttaaaa agccacgaga tcaaagaaaa tatagtcata
     1561 aaaatgaatg gaaagaagat ctattggctt atctaagcga aaaaagtctt gctagtgagc
     1621 cttatttgaa cctgtcaaaa aaagaattgg cttcacaact aacgatgccc aagcgaacac
     1681 ttgataagat cttaaatgag ttacgagagg agaataaagt cttctacaag gtaacacgag
     1741 gaagaaatgg cggaatgatc attgcttcag tcaaaacgtt gttcgcgagg gttattcaac
     1801 taagtaaaga aaagagacta gattattcaa caaggatcac agaggtattt ggactcaacg
     1861 agagcttagt agaagagaca tttagacaat tatcaacccc cttgaaccct cttaaagaag
     1921 tgaatctttt tgaagtagat acaggttgaa aaataaaagg tgcaaattgc cttatacaat
     1981 atatctatga tacttctgta ggtgttgctc cctttgccta gctacgtgta ggtggtatac
     2041 ttataactgc gtatgcagtc ttatgtatca tggaggaagt tgtatgtcgt taaaaaatta
     2101 tgtctattca catcctgtta atgtgtttat cattggtcgt ttgggattat ctgtggaagt
     2161 gttctgtgag ctttatggtt ttaagcaggg gactttgtct tcttgggtta ccagggaaaa
     2221 aacagttgct tctttgccga ttgagtttct tcatgggttg tccttagctt ctggggagac
     2281 tatggatcag gtatatgatt gtttgtgtgt gttagagcag gagtatattg agtataaaat
     2341 agctaacgag ttaagaaaaa ggaagaagta tatccagtag tagtttttat ttacttaaaa
     2401 aattggttca ttgagcaacc tatcgctgct aaagtagcaa aggaaaacgt taaaggaaag
     2461 aatttaggga agaaatcgaa atgaaatcgg acgactggat tgaacacacc tggcttcctc
     2521 tgaaaagaaa tagcaaacga aagaaatttt gggtattgca attatggcca ttaggaaaac
     2581 tttgtagtta cggggcacga cttattcttg atttaaccgt ttactcgatt ggagtatttg
     2641 gaacggctta ttacttgata aatgatccca ataggattgc agcatcagac atttttacag
     2701 tgctaatgac gttgattttt gtcatgctat tgtctatttt tactcaatta gtcatgcaat
     2761 ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag cctattcgat ttaattgttg
     2821 taaatgcaat tctagcgact ttaataaaac aaattgaaca atctggaaga acaacaaaag
     2881 gagtgaagga ttttttattg acgtttattc actttgtaaa tggacacatg tatttgtttg
     2941 aagtgggtat tattctcttt tttgtcgggt atgtatccac gaaacttatt aaacgttcaa
     3001 ttgaaaaaga aattgaaatt gacctgattc cgacacttct tggttatgag tacaatgttg
     3061 attttggaag atatgacgaa ttgatcaata cggatatcgt tcacacgagt atccatgaat
     3121 ctactgttaa gggcatcaga tctattttaa aaaatggcca ggaaatgaaa gcaatagatc
     3181 gaagaaaaga gatctacaat ttttatgaag tcgaagcaag cctacgatat ggcatcggaa
     3241 aattgtggtt agatggaagt aatattcgtc aaaacaaacc atacattttt tggaacagta
     3301 gtgatttaga acttccaatg aaagataaat aattatggag aatcgtttga aaatgattcc
     3361 tctatacagg attgtgctta tagttggcat ggagttaaaa accttaaaga aggagcagtt
     3421 attcaaagtg aaagaacgtc ataatcagtt actatgtagt aaaacgggta cgatgaacat
     3481 tgaataccga acagaatatt tagaattaac gaatcaaact gattttttgt tgattcgaaa
     3541 gcaacaagag caagaaaaaa attaatggct acttatagcc aattgattga tgaatgggaa
     3601 caattcaagg aatctaaaat tccagaacgt ctctaaaagc ctttatttgt aaagagcata
     3661 aggaaacgtg ggaacacttt ttaaaaaaag tgttccaata ggctgtgcct attacgtttc
     3721 tcaaaaataa atgtgggaac aaaatattta aaaataggat agtgaaagga tagtgaagga
     3781 tagtaaaagg agggtagtag gatagcaaaa aggataggct ccttttcgct atccttttta
     3841 aattggagga attaatatgg aagtaagaat aagaaatgtg gatgagaaaa tcgtaagaga
     3901 aattgatcga agatgtgacg aacggttctg ttgcaaagtt ttaaatctac tatcaaataa
     3961 ggtagaataa tagaaaaaga tagcaggagg aatgacgatg aatc
//
LOCUS       DACCXM010000173         3997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCXM010000173 DACCXM010000000
VERSION     DACCXM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.182"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(76..606)
                     /locus_tag="IVY61_002661"
     CDS             complement(76..606)
                     /locus_tag="IVY61_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7971720.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(788..1444)
                     /locus_tag="IVY61_002662"
     CDS             complement(788..1444)
                     /locus_tag="IVY61_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP7971721.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1571..2044)
                     /gene="dfrF"
                     /locus_tag="IVY61_002663"
     CDS             complement(1571..2044)
                     /gene="dfrF"
                     /locus_tag="IVY61_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP7971722.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2439..3830)
                     /gene="amt"
                     /locus_tag="IVY61_002664"
     CDS             complement(2439..3830)
                     /gene="amt"
                     /locus_tag="IVY61_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP7971723.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
BASE COUNT         1254 a          862 c          575 g         1306 t
ORIGIN      
        1 aaaaatctta aggattttcc cgccaacgtg gtcggatcgc tccgtcggtt taacagcttt
       61 tctgtttctc tctagttatc tacgcatata aatcaaatcg gttattccgt tataagtctg
      121 tgttttacaa agccttaact ttatttccct gtctatgttg cccaaaagac gaaccccatt
      181 tcctaacaga gtaggaataa cagaaatgta atatttatca atcatatttt tgcttaccaa
      241 ctgctgaaca agatttgctc cgccacaaat ccaaatatcc tttcctgctt cttgttttag
      301 ccgttccaat aataaaacag gattttcact tgtaaaatga atttgttggg aagaattgca
      361 ctctttatgt gttaatacat aagttgtaaa tttattatat acccattctt gcggagaaag
      421 ttcagtaaca acctgatgag aagtattcca ccccattaag acagtatcta tatctttggt
      481 aaattctgta taagtgtcga tgttttcatt gtcatttcca tgaccattta gccaagctac
      541 tccaccatta ccgtccgcaa tatatccgtc aaggctcatg gcaataaata aaactacttt
      601 tctcattttt tctcttttat aaatacaaac aggaatttat ttctcttttt tactcataag
      661 ggaacaactg attgctacaa ataaacccat taaaatccat ggaaatgctg ctcttataaa
      721 atcactaacc atactttatt cctccttaaa ttccaatttc cttagttcta caaactgaaa
      781 tttttgccta tgaatgtatc ttgttcttta tacgcaaaca ggcttctgtg tgcataatcc
      841 aatgtcttga aaacggcatt tgaattagtc cacaaatatc tttattacac caataatcaa
      901 tcagccaaat tgctttttca ttgtcgttta ctacattcaa cgattctaag tatccttttc
      961 tttctttcgg tatttttctt ttcaattcat cataagataa ccgttcaagc attttctcag
     1021 tgctttccca tacttcgaaa atatataaat ataattcttc cagattaagc tgttttgaaa
     1081 agtctgcaat ctgctgtttc atgagttcat ttcctgttgt aatgataggt gaattgatac
     1141 gctcttgata attgcctgca aaaaatactt gttcatcttc atttatcact gtatgcacaa
     1201 ctatatcttc aatgcggaaa atatgccaaa tagaataagc aatcgtctta ctatgataac
     1261 catcagcgtt tatgaagggg attgagttaa aatcttctct gcataattct tctttgaaag
     1321 atactaaggt ttgtattaac tgacttcgta aagtaagtaa agtatcaata cccctcttat
     1381 aagtatcttt cttttttatt tgtgcctgca ttgttttatt aagttcagac cattccttat
     1441 tcatattcaa tcctctctca aattctgatt ttatcgcccc atacaaactg gaatttgtct
     1501 tacaacaatt tatatatgca taaaaagcaa tatatttatg aaaacaccct aaatccaaaa
     1561 tttactcaat tcattttttc cttacataaa atgttctcgt atatttcact tcctcaccaa
     1621 gtgtttcccc tatcaaaact tcaaaatcat tgatatcaaa ttctggaaag aatgtatctc
     1681 catcttcaat atttaaatct acttctgtga tatacatttt atctactatt tgcaaagctt
     1741 ccttaaatag tccatatcca ccagatatgt atacatctcg tcctttagcc aataataatg
     1801 catcttctaa tgatttaact gaaactaaat tatctccttg atactctgtt gtggtggaaa
     1861 caacaatatt cattctatta ggcaacggat gaccgatttc ttcataagac tttcgcccca
     1921 taataaccac attacccgtt gttaactctc taaattgctt ttgttctccc tttattttcc
     1981 atggtatatt accattcttg cctataacat tattctttga cctcgcaaca atcaaaccta
     2041 tcatctattt catcctccat aatatgatgt cgtacctgtg attccatcaa gtgcaccaca
     2101 tcttaacaaa ctcaaaatta tctcgaacta accagctcga caaattcctt ttttgtcttc
     2161 atctgcatta tatcatattg ttataagcat tgccatttct tttctgcttt tgaaaagtta
     2221 cttatcagcc tatgttttgt aaagggtgat attcagcatt tctcacgcaa caagcccttg
     2281 acaaaccata gactaataag ttttaaggta atcaagcaga aatgaaatag tctgctgttt
     2341 cacggcaaca aaaaagagat accaacacac ttatgtagta tctcttagtc tagtagcaat
     2401 attcttttgt ttgcaagcag gtaaacatgg gctgtttctt aatatctttt tgtttgggaa
     2461 acaccttttt cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata
     2521 aaccatagca aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat
     2581 gtgggatatt catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaag
     2641 aaaaagtcca atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc
     2701 gccgatttta aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca
     2761 atcacttgaa tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat
     2821 gcaaagatcc ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca
     2881 tcaagtgcat catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata
     2941 cccgcaatga tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta
     3001 atggctgcca atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct
     3061 tttacttttg caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa
     3121 ccaaatgcat tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac
     3181 cagccaaacc aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt
     3241 ctttctccta aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat
     3301 ccagctgtcg tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa
     3361 aatccgccac cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata
     3421 acaagtataa tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga
     3481 acggtaatca cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca
     3541 ccatgaaaca tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt
     3601 ccaataacac cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata
     3661 atcgttgtta tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc
     3721 cgtgctaatc cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta
     3781 cataaaatca tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc
     3841 tcctttctac gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg
     3901 aattaatact tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct
     3961 ttttcgtttt tcacacaaat aaagacaaaa aataaac
//
LOCUS       DACCXM010000174         3985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCXM010000174 DACCXM010000000
VERSION     DACCXM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.183"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            88..918
                     /locus_tag="IVY61_002665"
     CDS             88..918
                     /locus_tag="IVY61_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP7971724.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1162..1411)
                     /locus_tag="IVY61_002666"
                     /pseudo
     CDS             complement(1162..1411)
                     /locus_tag="IVY61_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1602..1796)
                     /locus_tag="IVY61_002667"
     CDS             complement(1602..1796)
                     /locus_tag="IVY61_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971725.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2301..2486)
                     /locus_tag="IVY61_002668"
     CDS             complement(2301..2486)
                     /locus_tag="IVY61_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971726.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(3336..3677)
                     /locus_tag="IVY61_002669"
     CDS             complement(3336..3677)
                     /locus_tag="IVY61_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7971727.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3677..3907)
                     /locus_tag="IVY61_002670"
     CDS             complement(3677..3907)
                     /locus_tag="IVY61_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7971728.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT         1295 a          739 c          652 g         1299 t
ORIGIN      
        1 tatataattc gcgagcgctt tctttgaatt ttttaatctt tcgtagcaga atgagaatgt
       61 attaaaaata gattaggagg aattttgatg tttagacacc agaaagaatt gcagtttgaa
      121 gcaaaaccag atcgtccgaa tccattaatc gcgaaatatc tgcaggagct gatcggcggc
      181 caatatggcg agatgagtgt agctatgcag tacctgttcc aaggctggag ctgccgagga
      241 gaagaaaaat ataaagatat gctgatggac attgcaacag aagaaatcgg ccacgtggaa
      301 atgttggcga caatggttgc tcgtttatta gagggtgcgc ctgttgaaga tcaagaagca
      361 gcgatgcaaa aagatccaac aatcggtgcc attatctctg gaatgaatcc acagcacgcg
      421 atcgtagctg gcttaggacc tcgtcctgct gacagcgtgg gcaatccatg gagcggtgcc
      481 tatatcattg cgagtggcaa cttgttagca gactttagag ctaatttaaa tgccgaatca
      541 caaggaagac ttcaagtagc acgtatttac gaaatgatcg atgatcgtgg cgtaaaagat
      601 atgctgtctg ttctccttgc aagagatagc tatcatcaaa atctatggga atgtgcagtg
      661 aaagaattag aagaaaaaga aggttcaatc ttagtcccaa gtactttccc gcgtgacaaa
      721 gaacgaaaag acattgctta tgacttctat aacttctcag agggagacca aagtagtgaa
      781 ggttcttggg ctagtggcaa agcactggat ggcgaaggcg aatatcaatg gttgaaagaa
      841 cctaagatcg aaggcgctgc accaaaatta gaaccagtaa caccaaaaat gtatgggaca
      901 cctccattca agaaataaat atctacccgt agaaacaccc gttttttgtt ccacacgctt
      961 tgtgagcaaa actgactaaa gtcattaatc ataaccaccc ttcaaaaggg tggttttctc
     1021 tagagctata agctcaaatc accggccagc gcctaaagac gctggctttc atattttatg
     1081 ttcaagccat attgcctttg ccagttttgc ccacctctta aaaggtttat gtactactcc
     1141 ttagccctta aaaggatcgt cttactctcg tacactcagc ttatctaacg cactatcatg
     1201 tttttcttgt tcttgaatgt actttttgat ggtcgcttca ttttgtccta cttttgtcac
     1261 atagtatcct tccacccaaa agtgtcgata cccaaattta tattttagat ttgcacaatc
     1321 tcttgatgat ctcctgaata ttggctcgat attgattata aattattttt cttctatact
     1381 ttggtgtgaa tacaatgtgg tatttacaca tctactttgt acgggctaaa ctatttccct
     1441 ttattgctcc acccgcatag caagtggttt tgtatttcaa tcgctcggcg cgctcaatta
     1501 ggtctaaaga caataaatct aaaaacccgc tgcttcactg acagaaacaa cgggttttca
     1561 gaaaaaaatg aaatgaaaaa gatctcttac ggcaatctat tttatttttt tgatttttta
     1621 taaatggaaa atgcttcaag aagtccaaga aataaagccg tataaaaaca agaacctatg
     1681 aaatctttat ttattagata ttcaacagaa atcccgataa cactagccaa caatatattt
     1741 atcagaagtg taaaataagg atgcttttta tagaatagaa ctagtgttgt tttcatagac
     1801 tctcctttct tcatatttcc aattaatcac aaattcacgg cattactaga aaaattaaag
     1861 gggcgcagaa gtggaaaaat actcttcgac tttccctaca aaccaggcca tcttgtgttt
     1921 aataactata ccaaatctaa ggtacctcta aatctaaatt ccatttgtgg cggtgacctc
     1981 caatttttaa ctcttctatc tcccatccag tatacataac cttgatactt cgaccactta
     2041 tatatcgaac acacaaatac ctatccattg caggagtact acctaactta gttgctactt
     2101 gcatctaaat aacagctgaa aagataggac ttactcttag taaagtatct gaactattaa
     2161 tttttaaact atcattttta tcctcaacta catgcacatc tgttgcaact tcactttctg
     2221 tctcatccag ataaatgatt aaggaatcgg gaattgcaat atcttcagct gacgctggaa
     2281 tagttgagct aaattagtca tcatatcttt attataaaat aaccaacagc aaaaaataca
     2341 atgaaaaaca aacttggaac taatgtatca ttaataaatg ttgaaaaatt cagcaactca
     2401 ttactgtatg attttagcag cccaaccaac atatacccta taaaagctaa taagatggtt
     2461 aggaatcctt tagaattttt actcattcat agacacttcc ttaaataact tttattatag
     2521 aaggtattgt aaataataat attgacaaaa agtgactcaa aataaaaaaa accttacatt
     2581 atactggaat gaactgtatt gatagtgtac ctaaaagctc tactgacata agatcgtgta
     2641 atcggtaaag taattttatt aattatacgg gatgtaatca attagagaat aaactaatca
     2701 taaaaggtac ttttacattc ataattgtcg ctttgagatt ctactttggt tcactatctg
     2761 acccaattgc acattttcga tcattacaag tattggtgtc gttagaacaa ctaaataaaa
     2821 actagcggta gatttttcag aatctatgct aatttctgta tggatgttcc agtgatggca
     2881 aatttctctt ttcattgttt ttcttgtcgc atgatatgcc tttttgtgtt attcagttaa
     2941 actatagaaa ttctttccta gacagatctt gatcccaaat tttacgtttt tgagcatcga
     3001 tatagtttaa aatgacatta ctcatatcta aagagtttag attttttagg actaaaactg
     3061 tcagttgttc tttttcttct ttctgttaaa gtattgtcgc gacttttcgt atacgttaag
     3121 atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg aatgacttga ggaaatcacc
     3181 tcaatcattc gtttaaaatg ctatatattc tttagtttaa gatacggaaa ttaatgccat
     3241 cgaccacact agtagaatga acgacattta cttggccttt ttttaatttc gttatgttta
     3301 gcatataaaa atattataga gtttacttac aatgcttaaa acatatactg tataatctga
     3361 tcaattttag ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta
     3421 aaatcaagag attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt
     3481 aatgagtaac gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac
     3541 attgttttac ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt
     3601 tcttttccta cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa
     3661 attctttctt ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt
     3721 ctttctcata atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta
     3781 acgtaattgt tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct
     3841 ttccgttatc agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa
     3901 caagcattat catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat
     3961 tcccctttgt aaactatcta gaaaa
//
LOCUS       DACCXM010000175         3955 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCXM010000175 DACCXM010000000
VERSION     DACCXM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.184"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(442..708)
                     /locus_tag="IVY61_002671"
     CDS             complement(442..708)
                     /locus_tag="IVY61_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971729.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(719..910)
                     /locus_tag="IVY61_002672"
     CDS             complement(719..910)
                     /locus_tag="IVY61_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971730.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            1201..1623
                     /locus_tag="IVY61_002673"
     CDS             1201..1623
                     /locus_tag="IVY61_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971731.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            1640..2062
                     /locus_tag="IVY61_002674"
     CDS             1640..2062
                     /locus_tag="IVY61_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7971732.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            2091..2291
                     /locus_tag="IVY61_002675"
     CDS             2091..2291
                     /locus_tag="IVY61_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971733.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            2378..3266
                     /locus_tag="IVY61_002676"
                     /pseudo
     CDS             2378..3266
                     /locus_tag="IVY61_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            3404..>3955
                     /locus_tag="IVY61_002677"
     CDS             3404..>3955
                     /locus_tag="IVY61_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7971734.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNI"
BASE COUNT         1466 a          685 c          655 g         1149 t
ORIGIN      
        1 atcgcaaaga ttttctcatc gatatcaatt atcattccaa tgacattgac gttttctaaa
       61 gagcaattta acgtagctac tacattcgta tcttttatag ctctattttc gtttgctgtt
      121 cctaaaactt ttcaaagcaa atctagcaac gcaaaggtta atgtagaaaa cactgataga
      181 aaaaatgatg aaagttatta aaaaaaataa ttctttcatt tattgtcaaa cctttcttat
      241 agacattaga cttttttagt cagtccctcc cgactggctt tttcgctctg tactcagctt
      301 catcaagccc aatgaaaatc caaagcatgt aaacgatcgt gccgattaat gcttgtctac
      361 taccccatag acctaaagcg tagataatta gtggcgcgct gaatactaat gttcgattaa
      421 actttcccat gttgacctcc tttaaatttt gtatttagcc atcagttcat cgatgtcctt
      481 tttgtcatat cttaaaaagc catctatacg aatttctttt aaaccgtgag ctattaactt
      541 ctcaaaacct tgatcgttta cacctcccac atattttcta gcttcgatct tttttaaata
      601 tcgtgtttct gacaaattct tttttgaaag ttgactaagt gcgtccttta caacttcaac
      661 aattgcaatt cttaaacgag tcaaaaactc ttcgcttaga atattcatga cttgcctcct
      721 atcgaattct gtaatcacgg atgatctcta gaatagttct gtttgccttt ggtccactcc
      781 agtgtccatc aagaatttgt tgcattcgta cacgcgtgtg accataagca gtagccaaat
      841 catccatagt tattccattc tctttcataa actctttgat cgccgcacga ccgttatcca
      901 agtttgacat taacccactt ccttttttta taaatttgta agttaaaatg atagaaaaaa
      961 tataatttcc attgacattt ttacactata gagtaaaatg aatacataga gaaataagcc
     1021 tataacaaaa ccttttattt gcacttggtc gccaaacttt atgctataag gtgtgttttt
     1081 agtttgcttt tttctatcaa attaacttac ggataaataa taactctata tcgtaaaatt
     1141 gtaaagctat ttttactctt tttcgtaaat tattttttcg tgttggaaag gaacgttgga
     1201 atgacaacat ttgaaagaat aaaagaacta gcgaaaaaca agaataaaaa ccttcaacaa
     1261 gtcgctaaag atttgaagta cggagagaat tacttttata gtttaaacgc aggaaaacag
     1321 ccaactgccg aaaagttaca agaaattgct gactacttcg acgtatctgt agattatttg
     1381 ctaggacgta ctgagaaaaa aagatactat gatttaacag aaaaagacga gcgtgatatc
     1441 caaaaagaat tgcagtcatt aatcgacgat ttatctaatg ctgatggaat ggctttttcg
     1501 aagaaagata atgaaatgag tgaagcgaca cgtgaagctc tcattatttc tttagagaat
     1561 gctttgcgaa tagctaaaat agaagctaag aaaaaataca cacctaaaaa atatcgcgat
     1621 taaaaggagt acgttgttta tgtatttacc tcaaattgat aacaaaatta ataagctggt
     1681 aaaactatat caaacacgca atccttttag aattgcacga gaattgaaga tactagtttt
     1741 agaagaagat ttgggagaaa tttatggtta ctatagtaaa ttcaaacaga taaaaatgat
     1801 ccacataaat tctagtttag atgagatggg aaaatggata acttgtagtc atgaattagg
     1861 ccactgtgtt ttgcatccga atgaaaacac acctatgttg tctaaaaaaa cgctagtatc
     1921 tgaattaaaa attgaaaaag aagcaaatta tttcgcaact catctgacta ttgatccaac
     1981 tttagacggc tttgatcata tgacaaaata tgaaaagctc gtctgctttg gattaccaga
     2041 tgaattcgat cgtttcttgt gaattataat acaacgtaag gaggatatag atgaaaatag
     2101 gaatgagaaa accaagtata aaaaaatcca taagcgctag aaccactgga aaagcaaaaa
     2161 gaaaagtaaa aaaagcagtt attcctggtt atgggaaaaa aggtagcggc tggattaaag
     2221 acccaaaaaa ggctgcttac aataaagtat acaagaaaac tacattcagt ttttgggatt
     2281 tatttaaata aaaaaagccc gtgcagcaac acggactcga aaccttattt ctaagattcc
     2341 ccaataaaaa tcatatcata gaaatgagga ataaaaaatg aaaaaagtaa taacaagttt
     2401 attagttttt gcttctctgt tacttttcac tgcttgtggt aaaaaagtaa ctacagaaga
     2461 tttaaaagct aatgattggt tgattgaagc aaccaaagat gacgaaccta atatgatcgt
     2521 atcattttct gatcacgtcg tatcttttag tgttgacaca agttctatga aatcaacttc
     2581 aagtaacgat tgggaggcta tgggagaaga atttgctaaa agcttagtcg accaaatgaa
     2641 ttacaaattt gagtatacac ttgaaggcga tgttatgact tggaaagatg ataaagacaa
     2701 agataatgat gccaaatata ctgtcagcaa agaagataaa aatattattt taactccaga
     2761 aaagcctaac aaatctgatg ataatgagaa attagttttg aaaccctaca aaaaggaaaa
     2821 aaactaaaga atctagaaca actactagct caacaagtag caacgaacaa tcatcttcga
     2881 ctacaacaga aagtactatt gcatcttcta gtactgtaga acaagctgct attgcgcaga
     2941 atacgcctcc tgaaactagt agtacttact ctgctgaaag tgtagaaaac tatgcagaag
     3001 ctactcctag ttcaagtgtt gaacaagttt cacccactcc cagttcaacg gagcaatcag
     3061 taccacaaga ttatgtacca gctaatcaag ctcaaccaac ggcaccttct tcatctgaaa
     3121 ccgaaacctt gactttgcaa cctggtgaag ggaccaaaca aatagctgaa cgagctggaa
     3181 ttacggtaga tcaactgttt gcattaaacg gtatggatcc aaacaactac atgctttatc
     3241 caggacaaga actaagagtg aaataaaaaa agcacatttg ccgcccgacc aagaacgcaa
     3301 atgtgcaaag agctacggta aagtaggtct atttgttgta ccctatttta ccatctcgaa
     3361 atccgctgta caaccgaaca aacgtacgaa aggaaagata aagatggcaa aaaaagaaat
     3421 tgataaacga attaaaaagc acttaacaaa aaaaggcgaa gaaaaatatc agtttagtct
     3481 gtacttagga gttgatcctc taacaggaaa aaaaagaaaa acaacgagac gaggttttag
     3541 tacgccatta gccgctgaga gagctttaaa aagattggag gcagatatac aggaaaaagg
     3601 actgcaagct tctacagctc aaaagagcaa gaaattcgaa tatgtttacg gcttatggtt
     3661 tgaaaattat aaaaagacag ttaaagaaag cacttggtcc tccaccaaac aaatttttga
     3721 cacacacatc ttaaaagtgt ttggagataa gttcattgac aaaatagacg tttttttctg
     3781 tcaagaagca gtaaacactt ggtccgatag ccatcctaaa atttttaaga aaataaaaaa
     3841 ttacacatct aatgtttttg attacgctgc ttctttacaa attataacta gcaatcctat
     3901 gaaattagtt tctattccac gtggagaagc tctagatatc gaagataaaa atatc
//
LOCUS       DACCXM010000176         3903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCXM010000176 DACCXM010000000
VERSION     DACCXM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.185"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1455
                     /locus_tag="IVY61_002678"
     CDS             <1..1455
                     /locus_tag="IVY61_002678"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7971735.1"
                     /translation="VLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGY
                     AISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQ
                     QRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMM
                     AQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMN
                     LILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNR
                     YGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYA
                     VIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILE
                     TYAERKDVEKYAHLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQ
                     QVLEKELLEAISSLQTTPENEAWLQGALEVFKHE"
     gene            1448..2692
                     /locus_tag="IVY61_002679"
     CDS             1448..2692
                     /locus_tag="IVY61_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7971736.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(2828..>3903)
                     /locus_tag="IVY61_002680"
     CDS             complement(2828..>3903)
                     /locus_tag="IVY61_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7971737.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1308 a          653 c          787 g         1155 t
ORIGIN      
        1 gttcttttag cagatgaatc cttagaggaa tatgatacgg taagcaaaca aacgatgttg
       61 tatcgcgaat tactttcaga tgaagaatca aaagaagatc tgattgcaac aatcgttgat
      121 attttggggt atgcgatttc gccagagtat cttttcaacg tcttagcgga tcaagcaaaa
      181 caagcaacgt tccaattgaa tgatctaaac aaagcctttg tgcaattggc atctacctac
      241 aatcaattta atgggttatt tgatgatgtg gatctccaat caaaaaaact aggaacagat
      301 gagcaacaac gaaatgtgac gattaccgaa gtcattaaga aattgaatga cgtggaagtt
      361 ttaggtcacg atggggacgt gatcggggat gcttatgaat ttctgatcag tcaatttgct
      421 tcagaagcag ggaagaaagc cggagaattt tacacgcctc atatggtttc tgatatgatg
      481 gcacaaatcg tgacgttgga tcaaaaagaa cgacgattct ttagtgtttt tgatccaacc
      541 atgggttcag gttcattgat gttaaatgtg cgtaactact taactcaccc agataacgtt
      601 aaatatcatg ggcaagaatt aaatacaacg acatacaatc tagcaaaaat gaacttgatt
      661 ttacatgggg tagatgccga agaaatgaat ctccgcaatg gggatacgtt gaataaggat
      721 tggccaacag atgaacctta cacatttgat gcagtagtaa tgaacccacc ttattcggct
      781 aattggtcag cagatacgac cttcttagac gattctcggt ttaatcgtta tggaaaatta
      841 gcgccaaaat ccaaagcaga ttttgcgttt cttcttcatg gtttctatca tttgaaggaa
      901 acagggacta tggccatcgt cttgccacac ggagttttat tccgtggcgc tgcagaaggg
      961 gtcattcgtc aaaaattact agaagacggc agtatttatg ctgtgatcgg tatgcctgcg
     1021 aacttgttct tcgggacatc gattccaacg acggtgattg tattgaaaaa gaaccgccaa
     1081 actcgagacg tattattcat tgacgctagt cgagaatttg tcaaaggaaa aaatcagaat
     1141 aaattatcgg aagaaaacat tcaaaaaatc ctcgaaacct atgctgaaag aaaagatgtc
     1201 gagaaatacg ctcatttggc aaactttgat gaaatcaaag aaaatgacta taacttaaat
     1261 attccacgct atgtagatac gtttgaagaa gaagaaccaa ttgatatggt tcatgtcgga
     1321 aatgacatca agaagatacg acaagaacag caagtgctag aaaaagaact tttagaagct
     1381 atctcttccc tgcaaacaac acctgaaaat gaagcgtggt tacaaggtgc gctggaggtg
     1441 ttcaaacatg agtaatgata tacaaccaga aattcgtttc ccaggattta cggaagcttg
     1501 ggaacagcgt aagtttgaat gcttacttga taaaaaagat ggagtaagga ggggaccatt
     1561 tgggagtgcc ttaaagaaag aattctttgt atctaatagc aattttgttg tctatgaaca
     1621 acaaaatgct atttatgata attacgaaac aagatataaa attactgaaa aaaaatataa
     1681 tgaattaatc aaatttaaat tagaaccagg cgattttatc atgagtggcg ctggaacaat
     1741 tgggagaatt tcaagagtac caaagcaaat aaaaccgggt gtattcaatc aggctttgat
     1801 tagacttaga ataaataaag aaatcacaga ttcagaatat tttattcaat ttattcgtgc
     1861 agattttatg caaagaaaac tcacaggagc taatccaggt tcagcaatta caaatctcgt
     1921 accaatgtca gaagtgaaaa aatggatagt acaatttcca atattagaag aacaaaagaa
     1981 aattgggaac ttcttcaaac aactggacga cactatcgct cttcaacaac gtaagctaga
     2041 tttattaaag gaaacgaaaa aaggattctt acaaaaaatg ttccctaaaa acggagcaaa
     2101 agtgccagaa attcggtttc ctggatttac ggaagattgg gaacagcgta agttcaaaga
     2161 gtttagtaag aagactggaa aaaagaacac gaaagattta gacttccctg cctattcggt
     2221 aagtaataaa gcaggtttaa tttctcaaac agaacagttt gatggtagta ggttggatga
     2281 tttagaaaaa acaaattata aattcgttga accaaatgaa ttcgcctata atccagcaag
     2341 agtaaacgta ggatcaattg catttaacaa tcttggtatg acggtcattg ttagctctct
     2401 ttatgtagtt gtgaaaatga gcgaggactt agataatgag ttcatacttc agtttatcaa
     2461 gtcaccaaca tttatcaaag aggtcaagcg caacactgaa ggaagcgtac gagaatactt
     2521 attctatgag aatttcgcta atatcaagtt tccttttact aggaataaag aggaacaaca
     2581 aaaaatcggc gctttcttca aacaactcga cgacacgatc gctcttcatc aacgtaagtt
     2641 agatttgctg aaggaaacga aaaaaggctt cttacaaaag atgtttgttt agtctaatgg
     2701 tgataagtca agatgaaatt taagattttc tttattgagt aatatgtttt gaagcgcacg
     2761 aaactagacc cgatcaaaat caaatttttt tcgggttgat tcaaggattt gcgtcaataa
     2821 cggattgtta tttctctatc acactccttg gtggcgtata gagttggtgt ttacgtagta
     2881 gcgtatccac cagacgcgta aattttcttg cggttaggac gagtgcacgt ttgtgttgat
     2941 gctttggaac ctccttatat ttactttgat aaaaagcttt gtattcagga agataatttt
     3001 ttacagagtt ggcagcttca actaagtagt atctaaaata tcgatttcct tgttttgtca
     3061 gaggtgtatt ttgcgactga tagtttccag attggttcac tttccaactt aatccagcgt
     3121 attttgccaa ttttgtctga tcttcaaatc tttcgatttg accaatttca gcgattaaac
     3181 cggcagcata gactttacca acgcctggaa tactggttaa gcattgatat tctggaatga
     3241 caacaattac ttgttcgatg gctttatcta attctttgat atttttttca agtgctcgta
     3301 tttctcgtac gagaacactt aaaacaacat taattgattc ttgtgcaaga gcacctaggc
     3361 ggtaactcat ggtaattgca cgttgaattg ctttagcgat tttttctggt tgcttgaaac
     3421 ggccttttcc tttttcttga agtaaatcac agaaagcttc taaaggaagc tcagcaagtt
     3481 catccaatgt gaaatcttcc gtcatgagtg aaatgagggt tgcactaaaa agactcgtcg
     3541 attcgtctcg catttctcga gcaagcgtat tacatttgta cgttagattt tcaagaaaat
     3601 gttgctttgt acgaatcaat tgcttaataa gttgatagcg agtacgtgtt aaacgctgta
     3661 aggccatgta tttctcttct ttgatagggg aagttgtgaa tcgttgaata cgcaagaaat
     3721 cagcgattct taatgcatcg tttcgatcag ttttattctc atcaaacatg cgactaaatt
     3781 gtttcacccg aaaaggattt tcaatcgtta caagtgtgtt gagcttcttt agtttctcat
     3841 cttcatgaaa aaacatggaa ggatggaagc tgtacatgga cgttgactcc atgccaatca
     3901 caa
//
LOCUS       DACCXM010000177         3813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCXM010000177 DACCXM010000000
VERSION     DACCXM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.186"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..523)
                     /locus_tag="IVY61_002681"
     CDS             complement(<1..523)
                     /locus_tag="IVY61_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7971738.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSY"
     gene            858..1292
                     /locus_tag="IVY61_002682"
     CDS             858..1292
                     /locus_tag="IVY61_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7971739.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1315..1635
                     /locus_tag="IVY61_002683"
     CDS             1315..1635
                     /locus_tag="IVY61_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971740.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1776..1967
                     /locus_tag="IVY61_002684"
     CDS             1776..1967
                     /locus_tag="IVY61_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971741.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(2038..2295)
                     /locus_tag="IVY61_002685"
     CDS             complement(2038..2295)
                     /locus_tag="IVY61_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP7971742.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2453..2716
                     /locus_tag="IVY61_002686"
     CDS             2453..2716
                     /locus_tag="IVY61_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971743.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2740..2928
                     /locus_tag="IVY61_002687"
     CDS             2740..2928
                     /locus_tag="IVY61_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971744.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(3011..3196)
                     /locus_tag="IVY61_002688"
     CDS             complement(3011..3196)
                     /locus_tag="IVY61_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971745.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3411..3782
                     /locus_tag="IVY61_002689"
     CDS             3411..3782
                     /locus_tag="IVY61_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7971746.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         1366 a          560 c          791 g         1096 t
ORIGIN      
        1 agtaagaaga atctaattgg taacggccga tgtaacgtcc gttcgttgct gtgtaagaac
       61 cgcttgattc tttttgtgcg atccattctt ttgcagagct tgtacttgct ggagtagcag
      121 cagtttctgt tacagcagga gcaggttgtg tttcagcaac aggcgctgct tgtgtttccg
      181 tttgaaccgt ttcttgtaca acaggttgtt cagcttgaac aggttcagct tggactggtg
      241 cttcttgaac ggctgcttga ggttcagcag ctgcttgtgc agagccttct gttgggattg
      301 ttaattgttg tcctgagtag atcaggttaa tatcagaaat tgaatttgat tctgcaatgg
      361 cattgatcaa ggagttatca ccaacgtatt tttgagaaat cgttgataga gtatcacctg
      421 attgaacggt ataagcttcg tccgcatgag cagtcgttcc catgaagaat gcagcgccgg
      481 cagccaaagt tgttccaaaa agtaaagttt taagtgatgt catgaaaaat ctcctttatt
      541 cgttaaaaag ttttatttgt tgtgttcaac gaggaatact ttaacacgtg aaaatatttc
      601 ataggtaaaa gaagcatgac aatttatgac aaatccacgt tttttgtaac agaatgatgt
      661 tcgctcgatg tgaaaaatga tgaggaattt cacataatgc agaaataatg ggattagaac
      721 aggtgttctg atgagaaaag aaatgtataa ataacttgaa agaaattgct tttgttcatc
      781 attttattta catattgcgt atattttttg tatttttgct tgagatgtta caataaaaga
      841 aaaaggaggc ggttccaatg agtaaaagat accagaacat catggtagca gttgacggat
      901 caagacaatc gatcaaagcg ttcgctgaag cccttgactt agcaaaggac aatgaagccc
      961 acttatttat cgtgtcaatc atcaacaaag ttgagctgac ccatagtgcc tatgcctttt
     1021 caaaaatcta tgcagatgaa aaacaaagaa cagaagtagc catgctgaaa aaaatcaatg
     1081 atgccaaaga gtacggcatt gctgagatcc acgcaatagt tgaaactgga gatccaagaa
     1141 atttgattgc caatgtcatc cctggacaag aaaatattga tttgatcgtt atgggagcga
     1201 caggaaaagg tgctatacaa caagcattag ttggatcgac cgcttcttat gtcgtatcac
     1261 atgccccttg cagtgtgttg gtcgtgaagt aatgagataa gggaggaata gaaaatggtt
     1321 cgtcctcaag aagtccgtgc gccaaaagaa aaaatagaaa ttttagcaat tattgaagac
     1381 ggcacacaaa caaagaaggg atactcgatc gctttagtaa agtggaaagg aaaaaaaggt
     1441 gttgctctga gatgggatgg agataaccaa caagataaag gattccctgt cacagctaac
     1501 ggttatcatc cagcgtggtt tgttctgcct gataaattta tggaattata cagctatgat
     1561 tatgcaagag ccgcagcatc aatcaaagca ttagataaat taacagaaga gcagaatgaa
     1621 atggaggaaa aataaaaaag acgaaccaat cagcctttag aaaactgatt agttcgagga
     1681 gtaaaaatga aaaaaggtta tttgggatga aactagaata atgcgtataa cttaaataaa
     1741 acttaaagca ataaaatggc aagagggtga ggcaaatgga agcaggaaaa gtattacagg
     1801 aagtaaaagc actatgtgca gataaaaaat atgaagaagc gaaaagcctg atcgaaaaga
     1861 acaaagaaaa tttggaagaa aagtacccga tcgcacaaga atttatagaa ttaaaacaag
     1921 gaacgatcct tcaccgattc aaacattttt ttggtataac agaatagaaa aaacgattaa
     1981 agatcggcag aaatttgctg catctttaat cgttttcttt ttaaacatgc gagggattca
     2041 attctgattc ttatataaaa aatagatcca aataataaga aaaattactg aaagcgtcaa
     2101 ttcgatgatg tgtgttatta atacagacgt tgtttcccaa tgcctaaaag aaaaaatttg
     2161 ataagtaaaa tatacagcag gtaaagaggc cagtaaaact gaaaacttct tttttgtcca
     2221 ttttatgtaa tagaaaagaa tgcttatcat aataaaaaaa ggaatataaa aaacaaattg
     2281 accgatcgcg atcatactat tgcctccgat aaataaagtc catgatacaa aataacactt
     2341 tgtgcgaaat ggcgcaagca gttttgtgga tttatccggt aaaatctgta tcgattttga
     2401 aattagagga gaaacttgct ataatcaaag tgaaagaagg agtgggattt tcatggatca
     2461 tgtagtgtta gaaatcgatt tgcatttgat caataatatt cgggtgattt actatgttgt
     2521 aagtaacagt gtcgaacaac gtgtactgac gaataaaata aatggtattt tagcaaagaa
     2581 agacgttcac cgattcaata atggtgaagg aagttattat agtatcccag tagaaaaaat
     2641 tatttatacg acagtcaaag tcagagaaga tctagatacc aaaaaagcat atgaacctat
     2701 ttttacaact tattgattga cagcagatgg aggaaaaaaa tgaatccata tgaacaactt
     2761 attcaagatt tgatcgatgg aaaaatcgaa aagattgatg taaaaagaga agaattaatg
     2821 gcttttcgag aagcgtggct gaagttggag aatcgtaaat atgtggtggg aaaagcctca
     2881 ctgaacggca atgtgactta tcattacgat ccgacacgtt tattttagta tgaagtattc
     2941 agaaaagaac caaaggagga cgatgcagca gcattgtcct cctttgattt tacaaaagtg
     3001 aaagttttac ttatttgaat agatttttaa atttgttttt gacactgtcg atatttacat
     3061 cttttaaaag attttgggct ttctctttat aaggtcctaa ttgttcttta tgttcttcga
     3121 taaatcgttt tgcgccatcg aaattgccgt tcttaatcat ttccttgact ttttgaaata
     3181 attcttcttg attcattttg aacacatcct ttgctatcaa catgattttt tacatggaca
     3241 aaacgagaaa aactgattta tccacacgtc aataatatca aataacgatc atttttacta
     3301 aaaaaacact cgattctcgc ttctaaagta ggagaaaaat ctccaacttg ggacggatgg
     3361 aatttcttta actgacccat atactgaata tataacaagg aggtagaaag atgaaagtga
     3421 ttaaatattt gattggactg gcggcgatta gtggtatttt gctatttgga ctgaaatatt
     3481 atactgaagg aagttatgga gaaatccctg gtatacttga tcagctaaat ccattagttg
     3541 aaaaaggaga ggtctatgta aagacacaaa aaccagagga agtaaatgaa tatggtacag
     3601 caaggtatgt gcagaaagct gctgatgcca atgggaaaga acgaacggtt gaatttaatg
     3661 gtctttctgt gttaaaagaa aatcattatt tgaaaattag caataaagga gcacacatag
     3721 aaacgtatga ggaagttcct agagaaaaag ttccggagaa agcattagca attattgggt
     3781 aaatcagttt ttgtattaag aatacgcccg att
//
LOCUS       DACCXM010000178         3792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCXM010000178 DACCXM010000000
VERSION     DACCXM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.187"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(632..3781)
                     /locus_tag="IVY61_002690"
     CDS             complement(632..3781)
                     /locus_tag="IVY61_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7971747.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1056 a          789 c          625 g         1322 t
ORIGIN      
        1 aaattgatct atcaataatt atcaaactga gtcatataga gacaaaaatt tcaaaaatga
       61 cctgttaaat ggtcatcgaa tatcatttaa aatcaatacg acggagtcag acacggagtc
      121 aaaacaagcc cgctgagagt aaaaaatata tacagtccca taggtaaagt gccacaggac
      181 tgtatatttt tttatttgag attacctgaa atgattccat ttacttttta tctcattttt
      241 tcctactagt tagtatcaaa tactctccta ttttctcaat aaactatttt gggttacatg
      301 gtatctgaaa cagctaaaac cttgtcatta cagcaaaaaa tcactttatc tccgtttcat
      361 ttcagataca tttcacttaa gcggattcca aaaaaacaaa agaaaaagga acccctagtt
      421 agcctatgct ttttattggt tcctctcaaa acacggtcat ataacaacgg ggtggggtaa
      481 atgaatgaaa aaggtggagg aaacattatt caatagctgt cttaaatact tcgttataaa
      541 atgtgaacaa ataaaagaca tctatcccta ttttctacac ttccacttta ctaccatctt
      601 tgaatcgtac aattttctta actcttctcg tttacctcaa ttcattttca agtggaatca
      661 gctcatcatc taacatttta cgccatgctc gcttcatttc ttgtgggtat ttaaatggct
      721 tcgcttctgg atggttggct ttatatcctt cgtaatcctt actgttgata attgctttca
      781 tattaggaat tggatcttca cctggcatat attgcagatt agaagcatgt aattctttct
      841 cagaaacaaa ccactcattg gaaaagacat gaatcaaatc atcactcgct tgtgtaaaat
      901 agcgacgttt aatgacaaag atatcttcat tactatctaa agtccccaca tcaaacgcat
      961 ttaaaatttg tcggtaaact tctttgactc cttctgtttt attcaattta gccaaagctt
     1021 tttcaatttc ttctcgtgca tctgaactat tcgccgcata agttcctagt aattgatcaa
     1081 tataagtaga atcaatatca tacaacttcg tggtattttc actataaaat tcgatcgtgg
     1141 aaaaatcttc tccctctttt tctggttctt cctctattaa tgaacccttc acggtctcgt
     1201 acacgccaat ttgttcttcc aaaagttgaa tttgattgtt caaggttgaa gatttcacca
     1261 tgtcatcatt gtaatcgtca taagtcacta acgattcata agcacgattc aattcttgga
     1321 atgctttgac gtactcgata cggatttcaa gtggcgtatg ttcatcaatg gattccatgg
     1381 atgaaacctc ttttaactta ccatatgctt gcttgaatct ttttttggat tcctgataac
     1441 ttggataaat caaacccgat tcttcttttg cttctgaata cagttttgtc gcatcctcga
     1501 cattcttctc cattgtggct ggctttcgga aagtaacaat tagtcctttt gttttttctg
     1561 gatacgtacg gttcgtacgt gaaaaagctt ggatcaatcc tgcatactct agattacgat
     1621 ccacaaatag ggtttgaatc gtaggtgcgt caaaacctgt caatagacga tccactacga
     1681 tcactaaatc aatttgtttc ccaaattgtt tgaattccgc tctttttcgt gccaaacgat
     1741 tattgatgtc tccgttataa cgctcaatgt ctgctaatga ccaggaagta tcataatact
     1801 gattatattc ctcaataatt ttttgcattt cttcttgttg gacacttgaa tctcggctgt
     1861 tttcttctaa tgaataggta atagcgattc gtggaaaatc aggatcctcc atcgttcttc
     1921 cttcacgaat gggctgatta ggaaactctt ttgttaacca gtcaggttct ttcgtcattt
     1981 ctttgatcgc tcgataatac tttttggcca tgtcaatgga actcgtggtt aagatcgcag
     2041 acttcgttgg acggccattt cgaaaatcaa attttgtata ggcattgtct ggacggaaca
     2101 ttttatgaat gactttttgg atatgttcat ctgcttcaaa aacagtaggg tccaaataac
     2161 tttctttttt gacaggctcc atttgatcaa tcatacgatt gatctgttcc ggtgaatact
     2221 ctgcatatgt ttctacttct tttaatttcc gatagatttt attctctaat gaagtcggct
     2281 ccacggtgtt ttcatgctct acttgaaatc ctaaaaccga gccatcttct aatgcattct
     2341 tgatggtata ggtatgcaag acctctccat actgatcatg ggtcgtacgt gccaattgcc
     2401 ctttggcttg tttccgattc tcagggaaaa tcggtgtacc tgtaaagcca aaccaagtgg
     2461 atttcggaaa catttttttg atttcttcca tattttcagc acttaacgct cgatgacact
     2521 catctactac aaacacgata tgttgcccca ttaatttttg gaagcgattg gttccttttt
     2581 tctcttcttg tttttttgca tatttcaacg ccgcatctag cttttgacgg gttgtaataa
     2641 tcacaacatt tgcattggca tctgccaaca gagtttcact taactctttg gcacttcctg
     2701 ttcccacgat caagctattc gctttggcgt tcccggaaga aatcccagta ttaaattccg
     2761 aagcaaattt cgtaaactct gtggtggttt gattatctaa atctttgcgg tccactaaca
     2821 tgatcgtccg atcgatcccc gattttcgtg ctaaaagctt agtagataca aaactagtca
     2881 acgtttttcc tgaaccagtc gcatgccaca catatcctga ttgatgttta tttgcagcaa
     2941 taaacaaggc ttgaatcgca tgtacttgat aaggatgtaa caccatgagg attttattgt
     3001 cttgatcttc actgacaatc gtataatccg caatcaagcg atgagcatcc gggatattta
     3061 gcacttgttt gcaaaactca tataagtttt ctaccttttt attatctttc gttcgccaac
     3121 taaataaaaa cttaggatgc aaatcctttg gtaaggcatt tgcaaaatac cgagtggttt
     3181 gttcattgga aaccacaaac aactgtaagg tcgaaaagat attgttccga aacatacctt
     3241 cttctgcata ttttttgatt tggttaaacg cctgataaaa actatctttt gcggttactt
     3301 gtttcagctc gatttggaca atcggtaatc cattgatcag taaggtgacg tcaaaacgtc
     3361 gatcccgtgc ttctatgtta cttccacgtt ttgcaatttg atggaccact tcataggtag
     3421 agattcctcc accaatgtct tgattagaat aaagaatgag cgagacagaa cccagttgcg
     3481 gatcttcccg ttcaatcgtg atccgagcca ttccattttc accttttaac catttcgcag
     3541 catcaaaggg ggttttcgtt cgtagtaaca attctgtttt gatcgtttca aattctttat
     3601 cggtcaaagg aaaatcattc agttctgctt gattatttga gatgattttt tggcgtaaat
     3661 tgacccaaag atcttcttct gattttagat cgggacgata cgtccactga ttatgccctt
     3721 cccccaatac acggatcaac tgttcttcga cttccgcttc ctcacggtga ctaattttca
     3781 tttatcttcc ct
//
LOCUS       DACCXM010000179         3742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCXM010000179 DACCXM010000000
VERSION     DACCXM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.188"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            133..948
                     /locus_tag="IVY61_002691"
     CDS             133..948
                     /locus_tag="IVY61_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971748.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1074..1331
                     /locus_tag="IVY61_002692"
     CDS             1074..1331
                     /locus_tag="IVY61_002692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971749.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1583..2155)
                     /locus_tag="IVY61_002693"
     CDS             complement(1583..2155)
                     /locus_tag="IVY61_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7971750.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2171..2776)
                     /locus_tag="IVY61_002694"
     CDS             complement(2171..2776)
                     /locus_tag="IVY61_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP7971751.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3177..>3742)
                     /locus_tag="IVY61_002695"
     CDS             complement(3177..>3742)
                     /locus_tag="IVY61_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7971752.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
BASE COUNT         1230 a          705 c          563 g         1244 t
ORIGIN      
        1 gactagagaa ttaaataaaa aataattact atctagtacg aaattaatta cataggataa
       61 tatattaagg tgctggacta caagtaaaaa atactgtatg atcatgacag attcaagaaa
      121 ggagcaataa gattggttaa gatagcagga ctttctaaag gtggaaaaac cgtttcacaa
      181 ctttataaac aaaactacag agactggaga cagttcagaa aaaacattaa ggattcttac
      241 tttattctac caacagaact tgaaaaatat ttaccgaata acaaagctat taatttatat
      301 ctgtattact gttttaaggc aaagaatgaa ggcggggatt cctggcattc ggtcaatacc
      361 atagcagagt ctctaggagt gacaactaaa actataaaca cctggaataa aaccttagtt
      421 gatttaggta tgattgcacg tatagacgat aaccatctca gtaaatcgac aattttactt
      481 cctctctcta gttactataa attagccctt gatagttctt taaaggatgt taccgagagc
      541 actgtccatg aaattgatgg caacttggtt gcagtttatc atttgtttga atggagaaaa
      601 aatgaggctg atgaaaatta taacgttcct tataacacac tctgttgtgt ttttaaaaga
      661 aaaacacaaa aagatactat ctataaattt atcttggtcc aagatgagaa aataagtaat
      721 ttcgaattaa gcactccttc tagtaagatt tatgacgatg catatagatt tgaatgtcaa
      781 gatctagaag ccctcctttc cgcaaataat ctaaacaata tagaacatga aaactttgtt
      841 gtaaacacta gatttaattt agtttctgaa aaggactcag atttgttaga tttgctgata
      901 gagcttacta aaaatctaga taagaaagaa aacattactg tactataact agcatcggtc
      961 catcattaag tttggactat tagaacactt gctattacag aaaaaggtat gctagccacg
     1021 cttactattt attggtatca aacaagtatc ttaatttgaa aggaagatga tttatggcta
     1081 atttatatat agagaacaaa ggagacttga atgccattat tactttaatc gtagttatgg
     1141 taaaatcaat aggtaatatc acaataggaa tgctgtttat tatattagca gcagtgctga
     1201 tttttatgat agttatttgg ttcttaaatg aaagatctaa agacaatcaa caccgtaggg
     1261 atatggagat aaaatcaata gagtcaatag attatttatc gataataaca ctaaaaaagc
     1321 cacgttccta aacgtgactt acgctgaacg atatgtgttg tttggtagac gaacaaatcg
     1381 ttcattataa atatatgatg gtatcctaat atagtaagcg tggctagcat acctttaatt
     1441 actttacctt ttacagttac catacacatt ttttgagcag aaatcaagta aataaaaaaa
     1501 tattatattt cacaacatgc taatagactc atatctagaa cgcattaagt atttacctgt
     1561 ctggttcgct gagacttctt ttttaacttt cttcaatttt cttctttatt ctaaaaagcg
     1621 tactgcgact gaaaccggtt atagcttcaa cttccttata cgatttacct gaagtaagca
     1681 gttcatatgc gtgcctcttc ttcggtgtga ttgttgcttt cggacgtcct tctctgaaat
     1741 taggattatt ctttcttgca aacatttttc cttcttgggt acgttccaca atcatatcac
     1801 gttccatttc tgctacactc aacaacgtcc gtgtcaccat tcgccccatg gacgtatttt
     1861 caatcgttcc tagattcaaa accttgatcg ttacgttatc atcgagtaaa ggttcaatga
     1921 tctctaatgc ttctctcgtg ttacgagcaa atcgatcaag cttcgtaaca actatttcat
     1981 ctccaggctt cagaccagcc aacagctgat tgaattttgg ccgatcccgt tttgtacccg
     2041 taaatttttc tgaaaaaata ttcttcttct ctacgccgtt ctcaagcaac tgttgaattt
     2101 gttcttttgt actttgagaa gcggttgaga cacgtgcata tccatatttc gtcatttggt
     2161 ccacctactt ttacaaatca tctatcgtat actcagaata cccttcatag aaatagctga
     2221 catcgattcc tttcatatat aactcgcgat cgtcaatttt atcagtcaga gcattggaaa
     2281 ttagatagcg tatttccaaa tcgtttatgg gacttcgctc cattgcagaa agatagtctg
     2341 ctttatcgat taagttccaa tctacaactt tttgcaattc ttcctttaaa atgagatcca
     2401 gccaaattct agtacttcgg ccatttcctt ctcgaaatgg gtgagcgata ttcatctcca
     2461 catatttctt gataatctct tcaaaagaag tttgtggcat ctgatcaata tgctgtaacg
     2521 aatgttctaa atacataact ggtgcaaatc gaaaatttcc ttttgcaata ttgaccgatc
     2581 ggatttttcc ggcaaaatcg taaatatccg aaaatagaaa ttcatgaatt tcagataaac
     2641 ctttaaatgt accaacttcg atttcattta tttttccaca atcgtacaat tctttcgctt
     2701 tttgtttact gagtttttct tcaaccttag ccaactcaac ttgattggtt aatcccaatt
     2761 tattctctaa aatcatgcga aatctcctta actttatgac ataagtttat gaaacactct
     2821 caaccccatt atatcgcgat tttgtttatg tgtcaaaaac tattaagttt ctgacccccg
     2881 atagattttt caagtgtctc tattttgcac agtctagttg acctttgata cacctctata
     2941 tttatagaga atctaaaaca tcaacatggc atactcaaaa ttctaaatat tagggataaa
     3001 gaattctcct atacaatcaa aaatttcctt tatttcgaac tccggtcacc taactctatt
     3061 gatctcttac aagccaaaga atatctctat actacaaaga aacaataggg tgcctggttg
     3121 gtctctttgt gttttatttt agaatctaga acgtaaatat tcttgtcatt ctgattttat
     3181 ctaagaatat caattaaatc tttttttgca gcctctgaag cgggcattac agaaaagacc
     3241 agtccaataa ccgaagatac cccaaccgct agtaaaatag taaatagatc aagtgacacg
     3301 tttaccttta tcaaagaacc aacactataa gcaagagcca ttcctaataa atagccaatt
     3361 aaccctcctg aaagagttaa aatcaaacct tctaaaagga actgcagcat aatcgatttt
     3421 cgagtagctc ctagggctct tcttataccg atctcttttg ttcgttcaga tacagatata
     3481 tacatcatgt tcattacacc tacccctgcg ataaataaag atatacctgc tacagctgtt
     3541 ataaaataag tgatcgtact gaggatttga ccaattcctt tcgttaacat agccgtatct
     3601 aacacttcat attcccccaa atgtttcaaa ctaccttttt ctttcaattg gtttatcact
     3661 ttagaggtca ccttgtcagg ttcaactcct tcctctaaag ttaacgttag agacgatgta
     3721 tccttttcag acttaaaata at
//
LOCUS       DACCXM010000180         3652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCXM010000180 DACCXM010000000
VERSION     DACCXM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.189"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..792
                     /locus_tag="IVY61_002696"
     CDS             <1..792
                     /locus_tag="IVY61_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7971753.1"
                     /translation="QKWIELKFSIIQTWIDLKYNAITTWNNIKQFFKDTWKNIKDTAY
                     NTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLR
                     EVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMV
                     GKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKE
                     IHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            782..1522
                     /locus_tag="IVY61_002697"
     CDS             782..1522
                     /locus_tag="IVY61_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7971754.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1519..>3652
                     /locus_tag="IVY61_002698"
     CDS             1519..>3652
                     /locus_tag="IVY61_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971755.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWM"
BASE COUNT         1358 a          450 c          710 g         1134 t
ORIGIN      
        1 cagaaatgga ttgaattgaa gttttctatt attcaaactt ggatcgattt aaaatataat
       61 gccattacta cttggaataa tattaaacag tttttcaaag atacttggaa aaatattaaa
      121 gatacggcat acaatacgtg gatttctata aaaaatacca tgattaatac atggaataac
      181 attaaggact ctttctggaa tattgttact ggaattgtta attctgctga aaatgcatgg
      241 acaaatctaa aaaatggtgt ttcaaatgct attaatcggg taaaagaaat ctttaattcg
      301 ttaagggaag tcaatttatt tgaaattggc aagaacatca ttgatggact tataaatggt
      361 gtaaaagaaa agtggaatgc tttgaaagat actatcaaag gtattgcagg taatatcaaa
      421 aattggatta aaggagcttt agatattcat tctccatcaa gatggatgcg tgatatggtt
      481 ggtaaaaata ttgttcaagg tattatcgtt ggtattgata aagagcaaag taagttagat
      541 caaaccatga caaatttagt aaaaacaccg tctgttcaac ctgtagtaaa tggaataagt
      601 cacctaccaa ttacacaaac aaagcaaaat atatctgcta gtgaaacaaa agaaattcat
      661 ttacatttaa atgtttatgg agatttacct gattcaatgg ttcgaaagat tactacaaaa
      721 attaaaacag aattgacaag acaaatgaaa cgagatgctg gtgcagtagg aggaacatta
      781 tatgcaactt aagagaggac aattttttat taatcaacat tattcttctg aatttaatgt
      841 gtatattcaa aacaggcctg cttctgtttc agctagtcga gtaattgaat tgagagagcg
      901 agaaggaaat gattctatca ttattgataa aggctattac aaaaatgtga ctagaaagat
      961 tgagtgttac tataaagcac cttcaattga tatggtgcag gagtgggaag atcgaatcac
     1021 tgaatggtta gatatgtcgt cttatagtga ttttattctt tactatgatg aacaatatat
     1081 ttatcaagct gtagtaatag aaggacctga atttaaagga acaagaaaaa caggaaatat
     1141 tgtccctttt gaatttacag tgagtattcg accatttaag gaaaattaca atggtagatt
     1201 tactattcga caaacagaaa cttttgaaat ttataaccca gagaagtatt cttcaaaacc
     1261 gcttattaaa ttgagtggtt ctggagatgc ttctttttat attaataagg ataaatatga
     1321 tttgaaatcg ttggacagag aattatttat agattctaaa ttagaagagg cttatcgaaa
     1381 attagatggt aatttagaac atcaggatca ggtcacctta tttttagatt ttccattttt
     1441 atatcctgga aaaaatgaaa ttaaatggac gaaaaatatt cattcatttg aaataatgcc
     1501 taggtggtgg agaaaagtat gaaaccaaga atatatagtc ctactgaaac agatttctca
     1561 acgaatggct taggaatttt aaaggattgt acaagatgtg aaatatatga agtagctaat
     1621 ggaaaatatg aattagagtt ggattatcct ttaggaacta gatttgatga atattttgaa
     1681 aatgactatc aaataaaagc aaagccaaac gatcaagaag agtatcatat cttttttatt
     1741 gatgataaag atatagatac ttttttaaat acagtaacta tttatgctca gagtcgtaca
     1801 aatcgacttg gaagacgggt agtcactctt gtggaagtgg actctaaaac tggtcaagaa
     1861 gcgatatcaa ttattgaaac taaaatggat aaaaaatctg acatacgact ttattctgat
     1921 attacggctg tttctagcac aatctttgaa gcgagaaatg ttttaaattg tattgctggt
     1981 gaacaaggat cattgcttca atattggggc ggagaaatta aacgtgaacc atttaagctt
     2041 tctttgttaa agcgaagagg tcgcaataat gttggaacga ttcggtatgg aaaagatatg
     2101 tctggtttga aggtcaaatt agattggaca ggtataaaaa caagaattat tccatatgct
     2161 gatcctcaaa gtgaagtagg tacgactaat cgaatttatg gttcgccagt agatagtgcc
     2221 tatattaata actatcctga tgtctataca gaacatgttc agtttacaga agaacaagga
     2281 gtaaaagaca ttaagagctt aaataaaata gctaaaaatt actttaaaac gatcaatcct
     2341 ggctgtgata agcctaaagt ttctattacg gttgaatttg ataagttgac tgatagtgaa
     2401 gaagcgaaag aatttgcgaa gattagaaat tatggtctgt ttgatacatt taaaatatat
     2461 cataaaaaat ataatattta tcttgaatca aaggttagtg gactacaata cgattcttta
     2521 tctgaaaaaa ctttgaaatt agaagcagga gacattcaag ttgcttttta tcagcaacaa
     2581 gctgtaacta ttcaggataa attaaaagat tatgcaacga ataattatat gagtgatttt
     2641 aatgattatg tttcttcaat gattacaggt caaggcagtg caggaggata tgtagtttta
     2701 tggccaaaag aaaaaccttc caacatcttt attatggata atccagattt agaaaaggca
     2761 agagaagtac taagaatgaa taaaaatggg attgcttttt ctaagaaagg atggcggggg
     2821 ccttttaatt cagcttggac attagatagt atatttaatg ccaattttat acaaactgga
     2881 ttaatcaaag cagatatctt tcaaaattct tttaacaaga ctggagatgt attgaaatta
     2941 gttaatggac tactccaaat ttggaacaac aaaaagaaaa taatggagtt aactaaaaaa
     3001 ggaatggaat tttggaattc taagggttca attggaacga ttggaacaac tgattctgtt
     3061 ggtaatcctt ttcctggggc ttctactcca acccctattg aagataattc tttagttatt
     3121 cgtacaaatg gagacggcaa atatattttg atttctccta aagtaggaaa aggattagtt
     3181 ttattaggaa atggtaaagc aatttatttt ggagacttag atgtacaagg aaaacttaca
     3241 gttaaaggaa aagaaataat tcctggacaa ggtggtggtc catcgggagg tggagaatct
     3301 actggtgggt atcctagtga attaaaaaca gatgcagaaa agagagcttg gagaatttac
     3361 gatattttgt gtaataacgg atttacgaaa caatctgcat gtggaatatt ggggaacatt
     3421 caacaagaaa caggaggaac ttttgatcct gatactgttc aaataggtgg accagcatac
     3481 ggattagttc agtgggatgg ttcttcatat cctcttgttg gtccagcaac atgggatgga
     3541 aaagtttatg ttcaaaactt atttaatgct gcaggtatta aagaaccaat aacgagctta
     3601 gatgcacaag ttcgattgct tatttggaca tttacaaatg gacaatggat gg
//
LOCUS       DACCXM010000181         3617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCXM010000181 DACCXM010000000
VERSION     DACCXM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.190"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            30..239
                     /locus_tag="IVY61_002699"
     CDS             30..239
                     /locus_tag="IVY61_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7971756.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            232..381
                     /locus_tag="IVY61_002700"
     CDS             232..381
                     /locus_tag="IVY61_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP7971757.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            416..1200
                     /locus_tag="IVY61_002701"
                     /pseudo
     CDS             416..1200
                     /locus_tag="IVY61_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1484..2569)
                     /gene="xerS"
                     /locus_tag="IVY61_002702"
     CDS             complement(1484..2569)
                     /gene="xerS"
                     /locus_tag="IVY61_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP7971758.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2903..3136
                     /locus_tag="IVY61_002703"
     CDS             2903..3136
                     /locus_tag="IVY61_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP7971759.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            3133..3540
                     /locus_tag="IVY61_002704"
     CDS             3133..3540
                     /locus_tag="IVY61_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7971760.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         1273 a          499 c          643 g         1202 t
ORIGIN      
        1 tatatttgaa taaaaaatgt ggtggttaaa tggtggttat aacaaaaaat gaattaatga
       61 aattaggtta tgggaagtat cagtctgaga gaatcatacg acaggcaaaa gcaattatgg
      121 cttcaaaaag ctatacctat tacgaaaata gaagattagg actagtacct gttgaatctg
      181 tagaagaagt tttaggtatg aagatagagg tagaggagga ttttcagaat catggttaat
      241 tttaaaaaag ataaaaatgg gaaatatcat tttactgcta gtttgggatt cgacaaacga
      301 acagggaaaa gagtacaaaa aaacgtagag gggttaaaac agttaaagaa gaaaaaaagc
      361 gtataccgaa cttgtttcta attatggaaa agaagcagaa acttctcaaa gtactatgtt
      421 gtttgaggag tttttttata aattattcct accttattat caagggaaag tagaagaaag
      481 aacatataaa aatcggttat ctacaatcaa aaaaactttt cttatttctt taaaatggag
      541 ttaagagaaa tacgtccaat tgacattcaa aagtggcaaa ataatttatt gagcaaatat
      601 aaaaattctt atgtaagaaa catgtatggg atttttcaga tgtcattaga tcgagcagtt
      661 gttttgggac tacttgaaaa aaatcctgca aaagttattg gaaatgtgaa gaaaaggaaa
      721 actacagttg atttttggac aaaagaagaa tttacaaaag ttatttcaac tttttatatt
      781 gatgatttgt accaacattt tggttttatt tgtatgtggt tactttttat gaccgttatg
      841 cgtttgggag aagccactgc attaacttgg aaagatattg attttgaaaa taaaaacttg
      901 cgtgtgaata aaacactcta ttataagaga gccaaaacct atgagtttgt agaacccgaa
      961 acggcttcta gtattcgtac aatcgaacta gacgacaata caattacctt tttaaaagct
     1021 tggaaggagc gtcaagtttc aataggaggc atgtcgtttg tactttctta taatggaaca
     1081 cctactcaga aatatacaat ccgtcatatt atgagaagac atgcaacgat cgttggtgtt
     1141 catataatcc gcatacacgg attaaggcat agtcatgctt ctttgcttat atcacaataa
     1201 aaagatgcac ttcgaattaa agaaaggtta aaacatgctg atgtacaaac gactttagga
     1261 acttatggtc atttgtatct aaattcattg agagagataa caaatgatcc cacagattat
     1321 atccaaacta atttttctaa aatcaaaccg acacctattt acgataataa aaaataaatg
     1381 ataaacaaaa gtaggatcac accaaaagaa acacactaga aaaactataa tacaggcttt
     1441 tctagcgtgt tgtttcttat ttccgcctat ttatacatta tactcataaa gcatttaatg
     1501 catcgatcgt agccgcattg tctatatggg tatataaagt tgttgtattg gtacttgaat
     1561 gtcccaattg ttgggcagtt aataccaatg aatttgtctg ttggtagagt ctagtagcta
     1621 atgtatgacg cagtttgtga gggctgactt gaattctaaa gccttttgaa tattttgcta
     1681 ccatccgttc gattgcaata ccggatatac gcttggcttc tccttggtaa attgctagaa
     1741 ataaagcttt ttcgttttct gttactttat atcgttcttt ccgaatagct agataattag
     1801 ctaaatattc cattgcgaat gctgctatat tgacagcatc tcttttattt ccttttctag
     1861 ttactaccac attattctta tctaaagata ggtcatctaa attaatattt ataacctcgg
     1921 agagtctcaa tccagatcct aaaatcagtg cattgatcgc caggtctctt tctacatctc
     1981 gttgataata gatcaaagct cgtttactca aagtattttt atattcattg gcaatataat
     2041 ctagataatt ttgtgtatcc ttatctagaa ataatttttt ctcaatggca ttggcacgtg
     2101 cagctaacgt agttttatct gtgatggttg ctacttgatc cataggatta ttgtctaagt
     2161 atggttttcc tagaggatta ttggaacttt tcgaaagata tttaaataga acctttaatg
     2221 ccgtgatatt tctattgata gtagaagtag atagacctaa gttattatta tctttgagct
     2281 tagtttttgt tgtctctttt tttacatttt cttttgtgcg acgtttcaaa tgagatttgt
     2341 acaagtttag gtcataggca gttaactgtc ttatcgaggt gatagaagta tcaggaacga
     2401 tagactcact cagaagccat tcataaaaga gcttataatt ttttagatat tctaaagtgg
     2461 ttcgaggtga ttgatcttta tcctcacgat cttgaacaaa atcttttata taatcgggca
     2521 tatgaagtat gattgtatct attttttgat atatttctgt atttttcatg tcattcatcc
     2581 tttcaataag taggggagaa gagtatctct ataattggac tataccaatt atagaatgtt
     2641 ttgtcaattg gtatgctcta aaatttatta atttgtttca aaaaaagcat ataaacaggt
     2701 atacgtttaa taatttaaaa taaatggtat acataaatag ggaaggaaat agaagggagc
     2761 aatctaaatt ttttgaattt tcttaattaa ttatatagaa agaacaatct ttctatagag
     2821 ttagacatga gagggactat gcttgattta aaatcaacaa gcgtatatac tgtatataca
     2881 aagaaaaagg agggagaaga ctatggcaat taaagaaaga aaatttaggg aagtggggaa
     2941 ctcagtagta ttaactattt caaaggagtt tcttaaagaa aatgggttaa atcccggaga
     3001 tacggttttt ttagatgaag aaaaacttcg agcggcaatt acaaaaaaac aagccttatc
     3061 tgatgaaatt gatttagcaa tatctcaagc attaactgaa tatgatacta cttttagaga
     3121 gcttgtcgat aaatgagtat tcattaccta gaagccaaag atattataaa aatgaatgta
     3181 atccaaataa aaaaatactc tcctaatgaa ccccttggtg taaaggatgc gaatgcttta
     3241 gaaatgtgtg tagggcaact tcgagcctca gcttttggtg aagaggttta tccttcgatt
     3301 tatgaaaaag cagctatcct acttatacaa ttgataaaga aacatccttt tcacaatgca
     3361 aataaacgta ctgctttctt agctactttt gtctttttga aaatcaatgg acagctttta
     3421 gctattgatc aaaaagatgc tgtaaattta gcggtatata ttgctacgta tgataaggat
     3481 ttcgatcaac taaaatatga agtgatcaac actataaaga cacatacgca gcccatttaa
     3541 ggagttactg tatgtgtctt tatagggtta ttagctgcta tagcgaaata attgaagtaa
     3601 attacaaata gctataa
//
LOCUS       DACCXM010000182         3609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCXM010000182 DACCXM010000000
VERSION     DACCXM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.191"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(228..905)
                     /gene="nth"
                     /locus_tag="IVY61_002705"
     CDS             complement(228..905)
                     /gene="nth"
                     /locus_tag="IVY61_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP7971761.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(912..1610)
                     /locus_tag="IVY61_002706"
     CDS             complement(912..1610)
                     /locus_tag="IVY61_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP7971762.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1878..3062
                     /locus_tag="IVY61_002707"
     CDS             1878..3062
                     /locus_tag="IVY61_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP7971763.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3068..3511)
                     /locus_tag="IVY61_002708"
     CDS             complement(3068..3511)
                     /locus_tag="IVY61_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7971764.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          931 a          722 c          606 g         1350 t
ORIGIN      
        1 tactggactt attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc
       61 aataaaaata acaactttta tttttatcgc aataaattgt aataagttac catcctagct
      121 actgagtatg tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat
      181 aataacaaga taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt
      241 ttctttttta gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat
      301 agcaaaggac atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa
      361 atcagtgtat gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct
      421 tctacttcca tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc
      481 acgtgggtat ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct
      541 gtctttctgc ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca
      601 tcgaaacgtt caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac
      661 aaaccaatcg tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg
      721 ggtgtcggaa aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc
      781 tgggcactta agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca
      841 tgtgcttcgg gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt
      901 aacatattcc gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt
      961 tgagctgact gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct
     1021 tccacttgtt gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg
     1081 taattaaaac tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc
     1141 ggctgataat ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc
     1201 gggcgtccaa attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt
     1261 ttttcttcgt attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg
     1321 taaagattat atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat
     1381 ccatttgtta cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc
     1441 atagcaatca tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt
     1501 agaacaaata aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga
     1561 ttagatatga ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct
     1621 ccgctcaatc acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat
     1681 ttacttcact gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt
     1741 tttgatgcgt atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag
     1801 aatgttgaca aggaaacaaa attactatac actatttttg tttccaagtt aacattattg
     1861 cataaaggag tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg
     1921 tctctgtctt attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa
     1981 cgctgcctta ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg
     2041 tttatgctct agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg
     2101 ggcgtcatcc gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg
     2161 gtttaggaaa ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag
     2221 gagaaatctc caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca
     2281 aagtttttgg atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag
     2341 ggggtctatt agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt
     2401 tcttaaatgc cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt
     2461 cagtagattt aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga
     2521 tttcatccgt tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc
     2581 ttcaatctat cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg
     2641 gtttaagctt ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc
     2701 gattgctcaa aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc
     2761 tcgcctatct attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg
     2821 ggattatctg ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat
     2881 ccaattctgt ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat
     2941 ctctatcgcg cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc
     3001 ttccgacggt attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat
     3061 agtagcttca tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg
     3121 tgtcaaattc ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt
     3181 gatggagttg ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga
     3241 tttctttttt attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt
     3301 ttgttagctt actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt
     3361 taggatcagg gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg
     3421 caaattcttt ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat
     3481 attcttctat tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat
     3541 tgtttttttg tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa
     3601 accaagaaa
//
LOCUS       DACCXM010000183         3591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCXM010000183 DACCXM010000000
VERSION     DACCXM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.192"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            750..>3591
                     /gene="esp"
                     /locus_tag="IVY61_002709"
     CDS             750..>3591
                     /gene="esp"
                     /locus_tag="IVY61_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP7971765.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDIIENPGDNIPAGYHKVTFTAGEGT"
BASE COUNT         1329 a          549 c          717 g          996 t
ORIGIN      
        1 tatggattat caaaaattgg atcagttaat tgaaagagaa agctgggaag attattacgt
       61 gggtctaact ttttcagaaa agttaaacaa ctacaagtta atagattata agtatttgag
      121 tgaaagcaat taattattca tgtagagtac tagacacttt aaatatggac aattcaatta
      181 taaagaaaaa gaatcaataa tttaatgagg ctgtgacaaa tgtcattttt tgtttcgcta
      241 aaaacgagtg aaaaaatgtt gataactcca atcattcgtt taagattcta ggaatcattc
      301 agaaatgctg tgaaaccgat actttataat tataaagaag gtgatttcaa attccgaaga
      361 aggaatttga agtcaccttt ttacttatgt cacagtctca ttcagtaatg cgggataagg
      421 ctccttattc cgttatttga atactctgaa gtttaatcta agaaagggta atattttgaa
      481 aacatgcttc atcgcataga aatgaactta atttcagaaa gaatacatac atatcttttt
      541 atatgttttc agctgctgaa tggttaaaaa gcacaaatat ccgaaaaaaa catgaagaaa
      601 tgtacttttt ttgttgtaat gttcatgaaa ttctctaagt aaattagtta acataaggtt
      661 agtaagattc ctattctgtg aataatacgt atcagattta tcttttattc ttactaggaa
      721 agtaattatt aagggagttg atttgaataa tggttagcaa gaataataag agagtatttc
      781 tagaaaaaac aaagaaacga gtattgaagt acagtatcaa aaaattaagt gttggggttg
      841 catctgtttt gattggtgtt ggtcttgtct tgggaacaac tgaactagtt aaagcacaag
      901 atgaaatttc tccaagcagc ccacttgaaa cagcgataag ctccgtacaa gtaggtgaca
      961 aagtagcttc ggggaatact tttcaggaga atcctggtta tacgaaaaat tataactttt
     1021 cggatttaca atttagtcct caagaactaa ctggagacac actaaaaggc aatacaattg
     1081 gatttgaagt ttacggaaaa cataatattg cagcatcaac taaaaactgg gaaatccgtc
     1141 ttcaattaga tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat ccaaagaaag
     1201 gaattggatc ttctagacgg acctttgtaa gaattaatga ttcgctgggt agacctacaa
     1261 atatttggaa ggtcaattat attcgtgcaa gtgatggatt atttgctggg gcagaaacaa
     1321 ccgatacgca aaccgctcct aatggggtaa ttacgtttga aaagagccta gatgaaattt
     1381 ttaaagagat aggcatagac aatctcaaga ccgatcgttt aatgtatcga atctacttgg
     1441 taagtcatca agacgatgat aaaattgtac cgggtattga tagtacgggc tactttttaa
     1501 cagattcaga tgatttctat aatagcttag atgtatctga aaataatcct gaccagttta
     1561 aacatggatc agtaagtgca aaatatgaga aacctaatac tcaaacaaaa gatggttcag
     1621 gatccacagg tgctaatgga gctataatct tagatcataa gttaacgaaa aattataatt
     1681 tctcatattc atcttccgca aagggcacgc cttggtatgc taattacaaa attgatgaac
     1741 gattagttcc atatgtagcg ggtatacaga tgcacatggt tcaggctgac aaagtaacat
     1801 atgatgtttc gtttgaatcc ggaacaaaag tagctgattt agcaatcgag cgacgggaag
     1861 gtcatgaaaa ttatggcata ggttcaataa ctaataatga tttaactaaa cttatcgatt
     1921 ttgctaatgc aagtccacgt ccagtcgtta ttcgatatgt tttacaattg actaagccat
     1981 tagatgaaat cttagaagat atgaaagcaa cagcacaagt tgaagaaaat aaaccatttg
     2041 gtgaagattt catctttgat tcttggttgt cggatacgaa taaaaaatta atccagaaca
     2101 cttatggaac aggttattat tatttgcaag atattgatgg tgatggaaac cctgacgata
     2161 aagaagagag cggagacacg aatccatata tcgggaaacc tgaattagaa gaagtatatg
     2221 atgttgacac aacagttaag gggaaagtat tcatccacga gttagcggga acaggtcaca
     2281 aagcccaact tgttgataaa gaaggtactg tattagcaga aaaaactatc gctccaaatg
     2341 aaaaagatgg ggctccaatt tcagatactg tagaatttga atttacgggt gtagattcaa
     2401 gtaaattaat cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt tttgataaac
     2461 cagaagaagg ttcaaccgtt attaaggaat caccaaaagc ggttgataaa caaaccgtgg
     2521 tagttggatt taaaccagat gctaaagaat caattcggaa taataaaaac ttacctgaag
     2581 atgcagagta ttcatggaaa acagagcctg atacttctaa cgttactgat agtacgaaag
     2641 gtattgtaac tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag tttgctgtaa
     2701 aagcttctca agctatggaa aatgatgcaa catacgtacc tataacaaca actccagaaa
     2761 cgacagttca aagtggaaaa cctacatttg ataaaccaga tgttccttta gctaaagatg
     2821 ccttttcaat ttcagatgtc tataataagt acttcggtaa tgcaagtgtt gatgcaaaca
     2881 ctggtattgt tacattcact ccagctaaag gtgtaggaga atcggagccg attactggaa
     2941 caattcctat taaaattgtt taccaagatg gttctgtagg cacgaccgat ttagcagtaa
     3001 ctgtaagtaa agatatttat gaaaatccag gagaaaacat tcctgcaggc taccacaaag
     3061 taaccttcac cgcaggggaa ggaacaagta ttgaaagtgg aacaacagtc tttgcagtga
     3121 aagacggcgt aagcttgcca gaagataaac ttccagtgtt gaaagcaaaa gatggttata
     3181 cagatgcgaa atggccagaa gaagcaactc aaccaattac agcagatgat acagaatttg
     3241 tatcaagtgc aacaaaactg gatgatatca ttgaaaatcc aggagaaaac attcctgcag
     3301 gctaccacaa agtaaccttc accgcaggag aaggaacaag tattgaaagt ggaacaacag
     3361 tctttgcagt gaaagacggc gtaagcttgc cagaagataa acttccagtg ttgaaagcaa
     3421 aagatggtta tacagatgcg aaatggccag aagaagcaac tcaaccaatt acagcagatg
     3481 atacagaatt tgtatcaagt gcaacaaaat tggatgatat cattgaaaac ccaggggata
     3541 acattccggc tggctatcat aaagtaacct ttactgcagg agaaggaaca a
//
LOCUS       DACCXM010000184         3543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCXM010000184 DACCXM010000000
VERSION     DACCXM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.193"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..398
                     /locus_tag="IVY61_002710"
     CDS             <1..398
                     /locus_tag="IVY61_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971766.1"
                     /translation="FLLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEELYKE
                     FGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSNA
                     ISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            412..1101
                     /locus_tag="IVY61_002711"
     CDS             412..1101
                     /locus_tag="IVY61_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971767.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1226..>3543
                     /locus_tag="IVY61_002712"
     CDS             1226..>3543
                     /locus_tag="IVY61_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP7971768.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKI"
BASE COUNT         1195 a          466 c          747 g         1135 t
ORIGIN      
        1 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
       61 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      121 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      181 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      241 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      301 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      361 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      421 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      481 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      541 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      601 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      661 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
      721 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
      781 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
      841 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
      901 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
      961 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1021 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1081 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1141 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1201 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1261 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1321 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1381 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1441 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1501 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1561 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1621 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1681 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     1741 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     1801 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     1861 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     1921 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     1981 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2041 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2101 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2161 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2221 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2281 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2341 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2401 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2461 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2521 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2581 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2641 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     2701 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     2761 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     2821 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     2881 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     2941 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3001 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3061 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3121 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3181 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3241 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3301 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3361 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3421 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3481 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3541 tat
//
LOCUS       DACCXM010000185         3508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCXM010000185 DACCXM010000000
VERSION     DACCXM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.194"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1379
                     /locus_tag="IVY61_002713"
     CDS             <1..1379
                     /locus_tag="IVY61_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7971769.1"
                     /translation="EWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNLETFQH
                     SAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRWFAFPR
                     LVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSKKNNKH
                     KVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNTTFENL
                     TLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFTAILVK
                     VTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVGKFIRA
                     VRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGYAQVYG
                     KYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVISLAEIA
                     FPNEKIFR"
     gene            1395..2435
                     /locus_tag="IVY61_002714"
     CDS             1395..2435
                     /locus_tag="IVY61_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7971770.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2454..>3508
                     /locus_tag="IVY61_002715"
     CDS             2454..>3508
                     /locus_tag="IVY61_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP7971771.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTE"
BASE COUNT         1247 a          520 c          656 g         1085 t
ORIGIN      
        1 gagaatggag tgctgcacga cggatattca ttatattaat ggatgttatt gtatacaatg
       61 cagcaatata tagtagtttc ttactgaagt ttcgtggaga gatcccatta cgaaatttag
      121 aaactttcca acactcagca attattattt cagtgatttt tattgcgttg aacattttgt
      181 tgggtgcata tgttttttat aatcgaatga tcagtgacat cgtgtttgtc acagtcatcg
      241 gtcaggtatt gatgacacta gggatcatgg tagtgacttt tgcgggccgc tggtttgctt
      301 ttccacgtct tgttatctta ctttcatttg ttctcggtac gcttttctta agtatctatc
      361 gtattctcgt atataaactt tatttacgcg taagtcgaga taaaaaggtc gtcattattg
      421 ggttagaaaa ggatgtcgca ccagcaatcc aaaattttgg ttcaaagaaa aacaacaaac
      481 ataaagtcga agcagtcatt cttgataact attatgcaaa catcaaaaat atgattaaag
      541 acatcgatat tgtctatctt gcttctcata tcgaagaaac tgaaaaaatc aaaatctatc
      601 aattagttac taaaaatgaa aaaaaattgt ttttgaatac aacatttgaa aacttgacat
      661 tgatcaatcc aaatattatg aatttcgaag atgaaagtat tattgaagca tctggattta
      721 gaatccaacc tgaatatgaa ctattcaaac gtttgtttga tatcatcgtt tcacttgttt
      781 tattagttat tgcttcacca tttatgttat tcacagcaat tttagtcaaa gtgacatcac
      841 cgggaccaat catctataaa caagtgcgga tcacaaaaaa tcaaaaggaa ttttcaatct
      901 ataaattccg ctcgatgacc gccacagccg aagcaaaatc tggaccagtt ttagctaaga
      961 gcaatgatgc acgagtaacg cctgtaggta aatttattcg agctgtccgc tttgatgaat
     1021 taccacaaat cttcaatgtg ctgaaaggcg atatgtcgat cgtaggacca cgtcctgaac
     1081 gtcctttctt cgtcgatcag ttcaatgcag aaaaccctta ttactactta cggcataatg
     1141 ttcgtgctgg tatcactggg tatgcacaag tctatggaaa atatgtctct gattacaaca
     1201 gtaaattacg ttttgattta ctttatatca aaaaatattc attgatgctt gatttaaaga
     1261 tcttgctgca aaccatcaag atcctatttg ataaaatgtc ctctcaagga cttgaagaag
     1321 acgaaaatgt gatctcattg gcagaaattg cttttccgaa tgaaaaaatc tttcgctagt
     1381 tgggaggcgt aaaaatgaac aatccgaaat tcagcataat cgtaccagta tacaatgcag
     1441 aagcaactat tggtcgaacg attgatattc taaaagaatt ggattatgaa gagtacgaag
     1501 tcgttttagt aaatgatgga tcaacagatc aaacacagga aatcattcaa gaactgattg
     1561 aaaatgatga acgttttgaa ttgattacta cgaccaatca agggcccgga ttagcaagaa
     1621 acgaaggaat aagacagtca aaaggagaat atctcttatt cttcgatgca gatgacacgc
     1681 cagtaaaaac gatcttgtca gattatgatc atttgctaga aaagagtcca aaagcggatc
     1741 ttgttgttag ttcgtttgtt tttcgtacaa tggacgaggg aagaatcgtt tccgaaaaag
     1801 aaaacttagt tacagaatgt cactatctaa ctcacgaaga gtttataaaa gacatgtatc
     1861 agttaatgaa tgaacagttg atgtatgttg tatggaacaa atgctatcgt agcgatattc
     1921 tgaaaaataa ccaaattcta tttaaaactt ataatagttg tgaggatcga atatttaact
     1981 tacattatta tgaatactgt catcaagtga tcatgaaccc aaaagtagaa tatatttatg
     2041 aatttgaagg tgggaagggg atcactaatc aataccgacc taacaagttc gaaacattta
     2101 aagaattcta ttcattagca aatactgtaa cagacgatat taataaagac ggtatggcgg
     2161 cgcttctttt aaaagggaca acttcagtta ttttttctat ttatagtacc tctgcaagaa
     2221 cttcaaaaga gaagaaagac gaagcaaagc agatattgag tgatccagtg atccgagaag
     2281 ccaagcaaat tgctaagacg gattcttcag caaaaaaaat aacaaagttg ctttacaata
     2341 tgccagctcc actattttta tctgctgtaa aagttggaag cttcgttgaa gtgaaaatgc
     2401 cagggttaat ggctgctttg aagcgagtat attagttaat ggaggaaaat ataatgaaaa
     2461 gaattttgca ttttcaggga cgaatgggtt taggtggagc agagtctttc atgatgaatt
     2521 tatatcgtaa aattgatcgt acaaactatc aatttgattt tttgatttat gaagattatg
     2581 cagatgttca agactatcat tcagagattg aaagattagg aggccgtatt tttgttgtac
     2641 ctaatcctaa gaaaaatatt tttaaatatt taatagaagt gaataagtta ctaaaaaaag
     2701 aatctttttc tatagttcac aatcaggttt attttggcgg gggaattaat ttatggttag
     2761 ccaaaaagaa tggaatcaga cagagaattg ctcatagcca tgcaactgaa gatggaaaat
     2821 ctcaaaatat tgtaatgaat gtattgcgta aatttttaac caaattgttg ttacaaaatg
     2881 caacagacta ccttgccgtt agtcaagaag ctggtgaaag tctgtttcaa aaccacccct
     2941 ttgagatagt tcataatggt attgatttag agctttatag taaaaattca gaagctaagg
     3001 ttaataagag aaaagaattg gatatctcaa tgagtacttt tgttgtggga aacattggac
     3061 gattagagaa acaaaaaaat caatcttatt tgttagagat ttttttagaa atactaaaaa
     3121 tgaattctga ttcttacttg attttagtag gtgaagggtc acttagaaat aaactgaaag
     3181 ccaaagcagt agaattggat atagagaaaa gagtaaaatt tttaggagaa agaaacgata
     3241 ttcccgagtt attatctgtg atggatgttt ttgtaatgcc ttctctttat gaaggtctgc
     3301 caatggttgg attagaagca caagcagcaa aattaaaatt agtcttagca gatactattt
     3361 ccaaagatac aaagttaaca aataatgtgg attttgtatc attagatgaa acaccacaag
     3421 aatgggcaaa aaaagtaata aaagagccgt atgctaataa aataacacaa gaactaaaga
     3481 gttatgatgt ttcgtacact actgaaca
//
LOCUS       DACCXM010000186         3447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCXM010000186 DACCXM010000000
VERSION     DACCXM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.195"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1157)
                     /locus_tag="IVY61_002716"
     CDS             complement(<1..1157)
                     /locus_tag="IVY61_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7971772.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="IVY61_002717"
     CDS             complement(1160..3409)
                     /locus_tag="IVY61_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7971773.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
BASE COUNT         1119 a          647 c          494 g         1187 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacg
//
LOCUS       DACCXM010000187         3405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCXM010000187 DACCXM010000000
VERSION     DACCXM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.196"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..2303)
                     /locus_tag="IVY61_002718"
     CDS             complement(<1..2303)
                     /locus_tag="IVY61_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971774.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="IVY61_002719"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="IVY61_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          930 a          764 c          550 g         1161 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatg
//
LOCUS       DACCXM010000188         3372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCXM010000188 DACCXM010000000
VERSION     DACCXM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.197"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            373..1812
                     /locus_tag="IVY61_002720"
     CDS             373..1812
                     /locus_tag="IVY61_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7971775.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2107..2325
                     /locus_tag="IVY61_002721"
                     /pseudo
     CDS             2107..2325
                     /locus_tag="IVY61_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2574..2789
                     /locus_tag="IVY61_002722"
     CDS             2574..2789
                     /locus_tag="IVY61_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971776.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3006..3262)
                     /locus_tag="IVY61_002723"
                     /pseudo
     CDS             complement(3006..3262)
                     /locus_tag="IVY61_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          995 a          586 c          715 g         1076 t
ORIGIN      
        1 ctatggaatt gaatatcata gcaatgtatt tgaaaagcaa attggaaaac aaataggtgc
       61 aacaccataa atatgaaaaa attgggaaaa agtagaatgg aaatttgctt acgtctcacg
      121 agatagctca cactattaat ctgaagaaca atgaagtcga cttttagaaa ttgctagaag
      181 aaagtaaagc gaattatcac tagttactat agagtaagtt caaataaagc atctccaact
      241 tacatggtta catgaggata tcaaatcgaa attactcaag cttcctatgg ctagagtcgc
      301 gagaagttgt tgggcagtag acataggtta tattgttctt agctacacga tcattagaaa
      361 ggaaatccta ttatgtatag ttatcaagaa gatgcaaaag tccaaaaaaa tcgctggtgg
      421 attttgattg ctatatctat gtttaccttc atgtccacat tggatggcag tattgtcaat
      481 attgcactac ctaccatctc agatgatatg ggcataccaa tgaatcaagc ggaatgggta
      541 gtgtccgttt atctgatgat tgtctgttct tgtttgttgc tgtttggtaa aattggcgat
      601 agccgtggga aaatcaaagt atttcggatt ggtacgctga tctttgtctt gggatcgctg
      661 ttgtgtgggt tcaatcattc gctaggtatt ttattgttcg cacgggtggt acaagccatt
      721 ggtgctagta tgactatggc tacaaatgcc ggaatcatca cggaagtctt tcccatacat
      781 gaacgtggtc gggcattggg agcaataggt gcgtttgttt cattaggctc gattgctgga
      841 ccaggtcttg gcggcttgat tttgtcccaa ttttcttggg cgtacatttt ttggatcaat
      901 gtaccggtgg gaatcgtaac gatgttgatt gctgaaaaat tcttaccaaa agatatcacc
      961 atgagcggcc atacaatcga tggagggggc tttgctgcca ttgcggctac gattatcagc
     1021 ttttttggag caatcttttt ggggcaagaa agtggctttt tccatccagt cccccttgta
     1081 ctgttcgtaa tttcagtgat tgcctttgtc agctttttaa aagttgaaaa gaaagcagcc
     1141 tctccattga tttctttcgg tatttttaag aacaagattt ttaccatgag cttgattaca
     1201 gccttgttga ttttttcttc caacttcttt gtcaatgtag tgattccttt ttatctgcaa
     1261 aatgctcgcg ggttgagtcc tagctatgct gggctgttga tgatggtctt tcctttgatg
     1321 atggtcgtcg gggcgccttt aagcggctac ctgaccgacc gggttggccc ggagattttg
     1381 gtattagtag gcttgggctt attgtcggta acccaggtca tgtatatgtt tttgtcagtg
     1441 gactctcctt tgtggctata cattgtcgct actgccatca tgggtgccgg caatgccttg
     1501 ttccagtccc ccaataatac gatggttatg agctcggtca ctatcgaaaa cttgggactg
     1561 gcaggctcga tgaattcctt tgcccgaaat ttggggatgg tcagcgggat ttcgctttct
     1621 acgacggtct tatatcaagc gatgtctgct aagatgggcc agcaagtaac taactatatc
     1681 gcaaatcggc cggatgtctt tttatatgga atgaagatta cctttttagg cagctttgtt
     1741 ttgtgttttg ttgccttttt cctgactatc tggcgtatca aaaaacaagg aggtattcag
     1801 aaaaatgtct gaaaaaacca ctgttgaaga atttttggag gcctgcgtat gggtgagtgg
     1861 gatttccatg gatgatggaa gatgaagtga acttctaaaa gccgaaaaac tcaagtatta
     1921 tcttcaaaat actagtgctc aatgagatgc ttgatttgat cacaagaaga ctgcgaaagc
     1981 cgctggcaac cgcaagctag gtatcccacc gatggactac gttttagagg ctactggtta
     2041 tgaatacgga gccaacgctc tcatctgcat aagaatacat cacccagatt atgtaaagat
     2101 tttcgatact tctgtcaaaa aatcattaag atagataaat gatttccgtc tagtcaaatt
     2161 ggttcagaat gtagacacac agatggtaag aagtcactca caattcgaga atggacttgt
     2221 cctatttgcc gtatacatca taatcgaaac gtcaacgata gtatcaatat tttgaccaag
     2281 ggctatgaat acaagcattg ccttagaaaa catcaagaac cttaggaaca acggggatag
     2341 cttggttaat aagagatacc gctgtttagg cttgcaagta tactctttta tcaagaattc
     2401 cgtggcttta gtcatgagag gcttaatact atgaaaattt tctttagcat ttttgattta
     2461 ggttcctttt ctaattataa taagatgacc actacagtaa cctactgaaa tatataaaca
     2521 gccactgtag taccattttc aaagtagtct atgagaggag aatcttcatt cttatgaacg
     2581 ctggaattgc ggatatgaat ttaataaaaa aaacccttaa tgattttact tccaattcaa
     2641 tatcaaaagg tactggtatc aatttaagca caataaaaaa gctgaaaagt ggtgaaagat
     2701 cagtggaaaa gctgaatctt ttagatgcta taaaaataac ggaatttgct atgaaaaatg
     2761 gaaaagctga gattgaaata tggagataat tatcgtctag tgaattactt gccacttagc
     2821 tgaatttaac gatccttggt gcttgaaggg ggagattctt gggaatagag catacttgca
     2881 agcctatgtc tttactaaca acgatatctc ttattaacca agctatcccc gtagttctaa
     2941 ggcttttact tttatctaag caataatcgt attcaaagga aatttttgac ccttagaaag
     3001 tctcatttta tcagacgtga gcttaggcat tgctgaattt tgttaattcg gttttaggtt
     3061 gtgctttgag cataatatga aagtattctt tatcacgatt tcattcatct aaggtaatgt
     3121 gatgtgattc tgaaattctt tcaaaggcag tttttgcata agctgacatt acatcatcaa
     3181 tgacatgttt acaatgtttc actactaaaa taagggggta accaagaaga aaaactgaat
     3241 gattatttgt atctaattcc atggtatatc aaccatttcc tatatagact gattatgaca
     3301 tggattaaaa taaaaaggca ctatgtctta attgtcggtt ttcgagttac cgaatggcat
     3361 tcatcaccca cc
//
LOCUS       DACCXM010000189         3349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCXM010000189 DACCXM010000000
VERSION     DACCXM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.198"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            27..416
                     /locus_tag="IVY61_002724"
     CDS             27..416
                     /locus_tag="IVY61_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971777.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            420..1184
                     /locus_tag="IVY61_002725"
     CDS             420..1184
                     /locus_tag="IVY61_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7971778.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            1313..2260
                     /locus_tag="IVY61_002726"
     CDS             1313..2260
                     /locus_tag="IVY61_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7971779.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            2390..3130
                     /locus_tag="IVY61_002727"
     CDS             2390..3130
                     /locus_tag="IVY61_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971780.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1107 a          549 c          708 g          985 t
ORIGIN      
        1 ttataaagat aggttaggtg attaaaatga aaacggtcaa tcgtgtgttt tgtgccagcc
       61 acgtgctaag tctccttctc agtatatact taggtgtcta ttcgattcaa cagccaattg
      121 aggtagagaa cattgttttt cagatgtcgc tgacagatgg tttaatttta agcgtttttt
      181 tctttttgat actgtttatt ggtaatattt tcggtagcgt tgccagtttt ttgaattttt
      241 ctatttatcc actcctttcg ctagcattag gagtggtggg actttgttct ctgtgcttat
      301 ttcccgagcc attcagtcct gcatttatat tatttggtac actgaacctg ttccagacaa
      361 ctgttggagc atggttgcta tggcgatcag gaaatttaat gaaaataggt gagtaacaat
      421 tgaatccaat tatattgaaa cgttctgtcc atcatattcc agtgttagaa gttgttctga
      481 aagaattaag agaggaacgt attcccgttg tcatctatta tcacggatgg cagacaagta
      541 aggagttagt tttgacgcaa ggaagaaaaa ttgcgcagca gggcattcgt gtgatcctgc
      601 cagatgcgat gaatcatggg gagcgcaaac agccggtttc ttctattcca tcctatacat
      661 tttgggatag tatctacgga aatctgtttg aatttgacac acttatcaag catttagaaa
      721 aaagaggtct tctacatgat cagcttgcag ttggcggggt ttcgatgggg ggaatgacaa
      781 catgcgcctt gttggctaaa catcctcata tagtagcggg cgtgtgtttg atgggttccc
      841 ctgcaccttt ggaatatgga aaagaaatcg taaggcgcgc atcggaatat aattatgtcg
      901 taccaaaaga ctattttgat ttgatttcat gggttaagag ctatgatttg tcattgcagc
      961 cggaaaagtt agctggacgt cctctgttct tttggcatgg acaagctgac gaaaaaatcc
     1021 cttatataca aacggaacga tttgtagaaa aaaatcggca taagtcttac ggaaaagaaa
     1081 ttgtttttcg ttcctcaaaa acacagcgac atatggtaca aatacaacta atggaagaag
     1141 cttccacttt tttagcaaaa aatttgataa aaaagtataa ataattgaaa cggaaaatca
     1201 ctcatgacga tagaggattt tccgtttttt attttgggct ataataaact ctgttaggaa
     1261 aatttggtat aattgatagt aagaatgaca acatgttaga aaggaagaga gcatgcccac
     1321 tttagcagat gttgcaaaac gtgccaatgt ttcaaaaatg acggtctctc gagtcatcaa
     1381 tcatccggaa caggtgactg acgaattgaa agagttagtt ttctcagcaa tggctgaact
     1441 ggattaccgt ccaaatattg ctgcaaaagc acttgtcacg aatcggtcgc agatcatcaa
     1501 attatttata ctggaagaaa tcgacacaac tgagccctat tacatgaatt tgctgatggg
     1561 aatcgctaaa cggatcggta aagcgcatta ttctctccaa ttagttacaa atgatgcctt
     1621 cgacgtcggt tcttgtgatg gatacatcat cacaggtgtc agaaaaaaag acttcgattg
     1681 gatcgaccgt ttggaaaaac cagttgtctt gtttggggaa aattctgaag gatatgatta
     1741 tgtagacagc gataatgaat atggtacagc aaaagcaacg caatatgcct tagaaagaga
     1801 ttatcaaacg attatttata tcggtatcga agtggacgag ccttttgaaa tttcaaggga
     1861 aactggttat ttatctgtga tgaaggaagt tgatcttcct agtaaaatct tgcggtttcc
     1921 aaatcattcg acagaagtag aaagatatat gacagaaaac tgggcagatt ttacagataa
     1981 aacttgtttt gtatgtagtt cagatcggct agcactagga gcagtaagag ggattataaa
     2041 tgctggagga cacttgcccg aagaatttgg tgtgattgga tttgatgggg tatttttaga
     2101 ccaaatcagt tcacctaaat tgacaacgat caagcaagat attatacgta tgggtgaagt
     2161 atgtggagaa atgttgttaa aaaaaataga agaaaaaggg gcgagtcaag gttatcgtcg
     2221 tttttttcct gaactagtta tcagagagac aacgaattaa gtcttattac tatatatata
     2281 tttatgaaat tcatcgctat tttcttaaaa taatcagcgc attgctactg aaaacaatga
     2341 atctgtgcta ttattgagaa aagttccatt tttgagaagg atggtttgaa tgaaagaaat
     2401 cagacgaaaa catttccgtt ttcctgcgta cgatgatgag ttaggcgtaa aattaaccaa
     2461 cgacaagaag cgagtattat ttgacggaca agaagatctt ttgactgaac agaatcagtc
     2521 cccgtttgaa cagatgaaag aatttcattt cacgaacacg aacaccgaga aaatccgtcg
     2581 caagcgtcgt gcaacaaatg gattgaagtc agctccgcgt aaggaaaatt tggaaaagca
     2641 taaaggaaaa ttaccggatt ataccggcca ttaccaatca gaaatcacat cgacaggacg
     2701 aaaaaaactt tttggtgatt cctctcgttc tgtcagacaa agtgaaacaa ggacagagtc
     2761 caagcatact acacgaaacg catctgttga cacgaaggtg cgggagcgtt cttattttgt
     2821 tcctaaatat gtacccgcat cgattatccc agaccctaga gaaagcaaaa tttctgagga
     2881 tcatttaatc gaatcgatga aaaaaacgga tgactcttat ctgttgttcg atacagaacc
     2941 gacgccgtat caagaaaaaa gaaatggtga gccgtctgta cgaacattca atcgaacaaa
     3001 aacgttagag gaaacaaagc agtcagaaga aaaaaaatcg aaaaaaagcc atggtatctt
     3061 agaacgctca ttacaaggat tgatcgatga tcaaggaaac tctttgggag aaaatagcta
     3121 ttttaaataa gtgatataca aatttatatt cggctacagt tggaaaggaa acgctgcggc
     3181 gtttccttat tttatactct atccgaaagt ttatatcgta tcctcaaatt tttttgagtt
     3241 taagcgaatt ttttctccca ataagtaaga aaataatggc aaaataaaaa aacaaactat
     3301 tttcgcagat cgtttgcaaa aatagtttat ttttttataa agaagattt
//
LOCUS       DACCXM010000190         3331 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCXM010000190 DACCXM010000000
VERSION     DACCXM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.199"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(181..873)
                     /locus_tag="IVY61_002728"
     CDS             complement(181..873)
                     /locus_tag="IVY61_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7971781.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            958..1134
                     /locus_tag="IVY61_002729"
                     /pseudo
     CDS             958..1134
                     /locus_tag="IVY61_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1310..1879)
                     /locus_tag="IVY61_002730"
     CDS             complement(1310..1879)
                     /locus_tag="IVY61_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7971782.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1953..2276)
                     /locus_tag="IVY61_002731"
     CDS             complement(1953..2276)
                     /locus_tag="IVY61_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971783.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2430..3242)
                     /locus_tag="IVY61_002732"
     CDS             complement(2430..3242)
                     /locus_tag="IVY61_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971784.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          930 a          665 c          580 g         1156 t
ORIGIN      
        1 gttgttagat aagttgcaca tgtttataga atctaaataa taaattttta tgtaccgccc
       61 ctcaaagtga actgaacccc aaaagttgaa ctatttaatg gactgtttcc gatattctac
      121 tggagatagt ccattaaata gttcaacttt tggggttcag ttcaaagagg gggggatttt
      181 ttatcgttgt ggaatattta aataccaacg cttgtcatga aaatcttgtg ctccaccctt
      241 agtatttcct tctggatcgt ttgtcgcacg catcatgacg tatactttct tattagggaa
      301 atcacgcata ttgaaagata catgatagcc aacatttcct aaagtattat aagcttgatt
      361 tacgtctggt ctataaatcc catcagctct tactctagct agttcttttc cagtattgta
      421 gtccataatg aaaatatact cgtatttata attagcaatg tgccatccag ctacatgcaa
      481 gtttgcgttt tcgatttctc cgaactgatc aatgtgggcg taatttgttc catctgtcag
      541 cgtagaattt gcagcacctg ctcgagttgg atcaattact ggtttatcct ccgaagtagt
      601 tggattatca tccgtaaaac catgagccaa atcataggct agtttttctt tacttacacc
      661 catttcagaa agataaccgt aaggatctgt atgatcgccc caaatatttt gtgttaccca
      721 taaatgcgat ttgattcctg gttggttata aggagtgtct aatgttaatg gaataccata
      781 tttcattgct gaatctcttg ccaattcaac gtatacttta tagttctttt caaaagtttc
      841 tttatcatgt gtatgttgta actcgatttg cacaggactt ttggctcacg aggttgtcct
      901 actctacatc ttgctgatag atatgctgaa aggaaaacac caacattatg ttttgctttt
      961 tcagctagaa cgagacaagc aggaatacct ttagaagata tcaaaggttt tttaggtcat
     1021 aaagatgttt ccactacaca agtctatgcc catatttctc ccgaagtgaa aaaaagatca
     1081 atgaatcaac tagaaaacta tatagaagag caaataaaaa agcactcaaa ttgagtgctt
     1141 ttttaagctc actatcacta aaactatcac taattttatt tataagcgaa catgtttcta
     1201 tcactgattt tttaaatcta gcgaaaaacc cttatcttaa aggcgttcgt tcaattcttt
     1261 ggcaaggtct tcaaagcctg gcttgcctag aagtgcgaac atggttttct tatagctcat
     1321 gtagaaaaat tttcttgatg atgattttat ccatcaattg agattgactg ttttgctctc
     1381 ctatgatttc agctatgatt gtccgatttt gcggttttct tcttaactgg tagttgattt
     1441 tcccagtttc catcaactcg ctaagcatat ccacgactcc gccaaatccg gtctttttcg
     1501 taataaaacc tagtgctacc attcgttcat ctagattttt tgctgtaatc gtcacaccag
     1561 taatgagcga gtgctgtaat gtttgggcca gctcttccat cccttcagca ttggatacga
     1621 cggaagctgt tttgttaata ggttcttcgt ttatttcttt tggttctttc ctctccactt
     1681 tcactgtttg acgaaggaca tctagttctt gcaagagcgt ttcgtattgt tcttgggtca
     1741 gcatcacgcc agctactttt tcacgattaa atatatatac gccagttccc gcgtctcgcg
     1801 cttgagcaaa tacatccatc ggcgatcgtt tcacttctgt gatcgaagat gtgggaactt
     1861 ctaatttctt taattccaat ggatttcctc ctttactaat cgctttttat tatagcacag
     1921 actgacttgt tttgatcttt tttccaaagg ttttatcggt ttttgcctaa ccagtaattg
     1981 atatcggtaa gaagtctttc gataccaaat gaaggatggt acagatacgt tttttgatct
     2041 tcgttatggt ctagtggaaa aaaatgatcg atgatcacta gatctgcata ccgaggcggc
     2101 ttatttcgag tacatacatg tatagagatc cgttttcgat gtcttagcgt tttgttcagt
     2161 accccagcaa gtcttcttgc ttcttggtgt tcatagcgaa aaaaaaagac gattttcact
     2221 tcaggagggt ggacatctaa ataccctcgt tcctggagtg ttttcagtaa aagcatcccc
     2281 cactctactg cggggtgtgc aagtgcttgt tggatcgcta gccgaaattg atatagttta
     2341 tctttggaat tgggaaattt ttctaatgtt tcctgataat aacgaacttc ttctatcagc
     2401 cggtcatgga taccaattac gtaacgaagt cattcttggt aaagtacttt tgtcagcttt
     2461 gatttgcttt cgctctcaaa ttcgctattc agccaaaaca gcgaatcaag cttttcagtc
     2521 acaatcgtca atttccgata gtcttgtgtc tcttctatcc gtttccaccg cttttcagta
     2581 atctggttac agctcaagcg caaaattgct agataatttt tttcttttat tggcaaaatc
     2641 tgctcctctc taaatagcaa ttcaggaaac aattcaaaga atagtgttcg actgtctcct
     2701 aaaatctcga tttctgccgg atcctttctt tgcttcaata attggatgaa catctcagaa
     2761 aaggactgct ctccatcatc tgcaaaaaaa tgcggaaaat ctttcaaaaa gcaaaacagc
     2821 agatatctta cttttctttc tgaaccaaag atctgtgctt ttttataaga aaaagaaagg
     2881 tggtactcgc caaaccactt tctcaatcgc tcttgcgtag aacgatcgta cttctgctcg
     2941 taaggttcat tcaatagcag atttttcagt aaacaaaacg ggggtgattg gatcagaaaa
     3001 acaaacaaga gataaggttc gctatatcct tgatcataaa ctaactgaac ggttttctta
     3061 tgaagaatca gctgaatggg acagtcgctt tccgccaaac gtttcagatg ccgatccact
     3121 gttttaacat cgattttcag ttcttcagct aaatcagctt ttcgccagat caaatctttc
     3181 attagattga ttagtgaaaa taaattctgt gcattttttg ataatagttt ttgccatctc
     3241 atcgatcatt tctcacctga tttctaaaca tattgtttga tcaaaatggg ttcactccta
     3301 aaaagataaa aaactccctt tctatttatt t
//
LOCUS       DACCXM010000191         3213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCXM010000191 DACCXM010000000
VERSION     DACCXM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.200"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(553..2166)
                     /locus_tag="IVY61_002733"
     CDS             complement(553..2166)
                     /locus_tag="IVY61_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP7971785.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3206)
                     /locus_tag="IVY61_002734"
     CDS             complement(2307..3206)
                     /locus_tag="IVY61_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971786.1"
                     /translation="MLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANLYAE
                     FAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQ
                     EITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKI
                     QLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQL
                     AQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGFHSI
                     HHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1037 a          551 c          572 g         1053 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga acc
//
LOCUS       DACCXM010000192         2986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCXM010000192 DACCXM010000000
VERSION     DACCXM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.201"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(165..1475)
                     /locus_tag="IVY61_002735"
     CDS             complement(165..1475)
                     /locus_tag="IVY61_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7971787.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1487..2905)
                     /locus_tag="IVY61_002736"
     CDS             complement(1487..2905)
                     /locus_tag="IVY61_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7971788.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          932 a          618 c          464 g          972 t
ORIGIN      
        1 tttttaacga cttagttatc ttatcacgtc taaaaacatg tgtcaactct tttttggaat
       61 tttcataaaa tgtttcaatg aagcgcgaca acgttgttta atatatcatt aaaaaaaata
      121 aaggtcaagc taaaatatta tttaagcttg accttattat aatttcaacg attgtcaaaa
      181 gtattgtttg cagctgtgtt gccagtttct atagataaat cacagctttt gaaacataag
      241 ttttgtactg cttcgattcg cccgaattct tttgcagtta tttggcaatg agaaaagcag
      301 acatcttcta taggcttttc aggaaatgct actaaatcaa aagctcggga aggaagtgag
      361 taacctggag aaaggcatgc ctgtacgttt ttgatgtgta tgtctttgac tttcgtcatt
      421 tgctttgatt caggtatttc ttcagccact gcttgccaac tttctggcat ttgagcaagg
      481 tcattgctat tttttctatt gtattcatta tgccagtcca ttatccaaga aaaaggaaat
      541 tgtacatcaa tcatattcag attttctacc ctgatatttt cgatcactcc ccctctttct
      601 tttgaagatt tcattcgaat accacagtct gactgaaaaa aatcgatatc gtgaatatat
      661 acgtcactaa ctccagcact aacttcgcta ccaatagtta caccgtatcc tgaacggatt
      721 ttacatctag caacctcgat tcttgatgat tttcgattga ctcttgcacc attgccatct
      781 ctgccagact taatagcaat acaatcatca ccacaggcca aatcacattc atagacacgc
      841 acatttgttg aagaatcaat atcaatccca tctgtacttg gtccatcatt gttgcggatg
      901 attacttttt ctactaatac atcgttagaa taagtgattt gcaagttcca aaaacctgat
      961 ttctgaaaaa tcaaatcacg aacaacaaca tgttctgcat gatacagtaa acaagcgcgt
     1021 ggacgtttaa tagcataatc cgcaatccaa cgaagtccct tttgatcgta ttcagctctt
     1081 gtcccgcttt tttgatcttt tccccaatac agctcccacc aatggtctcc ttgaccgtcg
     1141 attattcctt ttccttctat aagaatatct ttcgccgaga gaacattcaa aatcgctgct
     1201 ggccatttca tttctacgcc ggctactcgt gtatcgattt ctggaaatgc ttcgatatct
     1261 gttctcccct tgaggactgc accttcttca aagataagat gactcttatc ttttaaaaaa
     1321 agggctccga ctaaataaat gccttttgga ataaatacag gtaagtgttc tttcgcacct
     1381 tgatcgattg ctttttgcag tattattgta tcatcttgtt gagtgtcgtt tgttttaaag
     1441 ttcaaagcat tgataatccc tttttttgtc cgcaatacgt ttctccctat ctttcattga
     1501 tttttttcaa ttcttcacca ataaatacag tttttgaacg attcagtggg taacctacta
     1561 gcaaaatgat tgcggataca aataagcaga atgctggtaa caagtttgcc atcgtataaa
     1621 tcctttcttg tactaaaact gattggatgg ctccacctgt tgtagacgat tggtaaccaa
     1681 tgagtgccag cataaatccg cctaccgctc ctgcacatgc ttgtcctact tttctcgcaa
     1741 aagaatttac tccatatact gttccatctt ctcttaagcc tgtcttatat tgatgataat
     1801 caatcacgtc agtaatgaat gcccagacca tcaaattgaa taaaccagat cctaaagtag
     1861 cgataaataa aagaatcaga tatacccatg catttgttat atgaaggaaa tacatcagca
     1921 agtacataca tgaagaagca agtaacgcaa atacgcttgc ttccttttta ccaaataatt
     1981 ttgtcagctt tgtagcaaat ggtgctaaaa ctactactgt tccgtaagta aataaaagag
     2041 caacagacat tgcttgttta ttatggaagt aatcattgaa taaataagtc atattcgtcc
     2101 cagctaaaat ctgattgatc acaatgaaaa tgtcaacaat gactaaaata atcagtgctc
     2161 gattagctaa caaacctttt accaactgag aagcagatac tttttcgttt ttgacaactc
     2221 ttactctttc agtagtcaat ttacaagtta acatatatgc gacaaatgct attgcagcac
     2281 atccgattga aatccaaaag aaatgattcc ctgaaacgac ttgttgtcca ctcttgttat
     2341 gggtatacat taaaatcgga atgaagaatc ctgtagttga tccgccaatc gcagaaccaa
     2401 tgcttcggaa cgtggacaac gaggtgcggt cattaggatt tgagctaatt gctgctgcca
     2461 ttgctccata cgggatattg attgttgaga gaaaaatccc ataaacaatg tatgaaacaa
     2521 aaatataagc aatcttcacc gtcattggaa gatgttggac gaatggcaaa aacaaaatga
     2581 cagtcactaa gcaaaatgga tacttcattt ttctgatcca aggaataaat cggccagttt
     2641 ctgttaactc tgagacatca cataatcttc caaccatcac atcagcaaac gcatccaaaa
     2701 ttctcgaaat caagaataac gtacctacga ttgcccctga aacgccaaga acattcgtat
     2761 aataaatcat caagaaacta ttgactaaac ctaaaataaa acagttacct agatcaccga
     2821 acatgtagcc tatcttatcg gaaatcccaa attccttttt ttcttttttt attgtatttg
     2881 ctgtaacagc attttgtgaa agcatttgaa ttcctcctac ctcttctagt aaacgttttc
     2941 aaaattataa ctcttttttt cttgttttta tattcacaat gttggc
//
LOCUS       DACCXM010000193         2923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCXM010000193 DACCXM010000000
VERSION     DACCXM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.202"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..362
                     /locus_tag="IVY61_002737"
     CDS             <1..362
                     /locus_tag="IVY61_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7971789.1"
                     /translation="LPPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERI
                     ERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGRREKLPEWAIDQEAY
                     LKKKALERANRQSKAPF"
     gene            374..643
                     /locus_tag="IVY61_002738"
     CDS             374..643
                     /locus_tag="IVY61_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971790.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            806..1105
                     /locus_tag="IVY61_002739"
     CDS             806..1105
                     /locus_tag="IVY61_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971791.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            1105..1407
                     /locus_tag="IVY61_002740"
     CDS             1105..1407
                     /locus_tag="IVY61_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971792.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            1447..1812
                     /locus_tag="IVY61_002741"
     CDS             1447..1812
                     /locus_tag="IVY61_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971793.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            2439..2621
                     /locus_tag="IVY61_002742"
     CDS             2439..2621
                     /locus_tag="IVY61_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971794.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            2634..2828
                     /locus_tag="IVY61_002743"
     CDS             2634..2828
                     /locus_tag="IVY61_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971795.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT         1054 a          462 c          654 g          753 t
ORIGIN      
        1 agttaccgcc ttcgaaaaaa tcgaaggcta agcccgtccg tcataaatac ggagagtata
       61 aaaatgttct tttgtcagat gagcagatgg agaaactcaa aacagaattc cctaatgatt
      121 accaagagcg aatcgaacga ctgtcagagt attgtgaatc atctggtaag acttataaaa
      181 actatttggc aactattcga agttgggcaa ggaaagaaaa aagtgaacct aagaacgcaa
      241 gcagtggata caagcgcaca ggaagacgag agaagcttcc tgaatgggca atcgaccaag
      301 aagcctatct caagaaaaaa gcgctagaac gagctaatag acaatcaaaa gcaccattct
      361 aagaggtgga aaaatgaaga tcgattatct agaactaatt aatgaaatag cgaattataa
      421 aaagggcgag gaattagacg tcctgagaga cgtatatgat caactcgaag aagctggaat
      481 tgaaggaatt aagaatgatc gttcgagttg gagtaaactc agatactatt tcgcactcta
      541 tatcgatgga acgcaattaa gaaatttagc atataccaaa ttactattca ttgattgtgt
      601 taaaggcttg caaaaacatc ttaatgaact tgagcaggtg taaacaagat ggacctaaag
      661 acatttacag cacagatcga attaatgcat caagaagctt taagacaaag tgtgtcgtac
      721 gaagacaagt ggctcaacac atttcatggc ggacgtgaga gcgcacttga tcaagtactc
      781 aaattactga aaggagaatg tcgggatgga taagaaagcg gcaatgcagc gaattatcga
      841 attgacttat tcagaagatt ggcaggaaga taaaaaaata gttgcagaag tccaaaagct
      901 cggtaaatca atgtggactg aaaagcccaa acggagaacg ccgagaaaga ttgcaatctg
      961 gcatggtgat cgaattctag taacgggtac cgctaaacag ttatctgaaa ttactggtct
     1021 gagcaaaaat attatttggg atagagctag gagcttatgg attgattcaa aaggaagaca
     1081 gtttaggtat gtggaggaga aataatggac gaactaatcg cgaaagtaga gcagtgggca
     1141 aaagataagg gattggatca agcggatttc agcaagcaaa tgttaaaaac ggtcgaagaa
     1201 atcggggaag ttgccgcttc tctagctaga aaagatgaac atggtttaag agacggaatt
     1261 ggcgatgtag tagtaacctt gatcatcttg gcaatgcaaa atgatatgga tttatacgag
     1321 tgcttggatt ttgcttatga cgaaatcaaa ggtcggactg gaaagatggt caatggtgtt
     1381 ttcgttaagt ccagtgacct gaaataattc cattaatgga agaaataact gaataggagg
     1441 atagacatga ttgatgcaaa acaagtgcaa aaacagaaag atggaatgtt gatgtttgaa
     1501 gcatatgttc taccatttct taatcaattt gaagttttgg aatgctctgc cagcggagaa
     1561 gaattggagt atgttgtaat tcgtgaaacc aaagaaaacg ttcagaagct aaacgaattt
     1621 ctttgcacta ttaactgttg ggatatgata gctccaggat ttttatgtcc agctatggga
     1681 gaatttttgg aatattgtcg tcttgaagat gcaggcactt tggatttagc ttatctggta
     1741 tacaactacc taaacatcaa cacagatcat ttgtggtttg gcactgccga aagaaaatgg
     1801 gtagtccgct aaccaccaat atcaatgaaa aaaggcggtt tcctgaaagt ggaattgccg
     1861 ccattgctgt aaggttcttt acgcgactca aggtcacaga aatataaatg caaatctctt
     1921 ttgaagtgat ccaaagctct cggaaaatgg aagttttttg tttcaaagaa taaatgagat
     1981 ttgcgtcaag aacctgagag ttatttccaa aaaagcttcc aaagaaccga atgagctaag
     2041 gacatcatgt gtaaacacat aatatagctc tcctttcacg gctaatcata acatgaaaaa
     2101 tattcgatgt cagtaggaaa aaaggtgaat tatgaaaaga atattaaatt atcccggcag
     2161 taacagattc tgcaattaaa ctccgaagct gatgtatcag tatcaactgt agaatattat
     2221 gagcgtggag aaagtcaagc agtcacaaat gtaaaaatcg ataacgtcaa cggtcttatc
     2281 tgtttagatg aaggcgatag tatgaatgaa attgctgcag accatgaatt atcagaaact
     2341 gctttaattg atcaaatcga attagctaga caatactttg ttccatatgc agatacctgg
     2401 tccaagcata agaatgacat catttttcca gaggaacaat gagagcaact gatccagtaa
     2461 ttatccttga ggaagccaaa tttatttgga ctcacgaaga gatagagcaa gcacgcttgc
     2521 tcttttctca aggagttaag ccgagcaaag tagctgaaat aatgggtcaa aagattcttg
     2581 atgtcggatt gcttttgctc catctagcag aaaaaatttg atttgaggtg aaaatgatga
     2641 ttcaacttgc aggcatccaa acagggaaaa tttatttttc tggagaaagc aaaagcgaag
     2701 ccaaccaatg gctattgaaa acttacacga acaataagaa gctaaggaaa aaatatcctg
     2761 atttgttgct aaaggatgat cagattatgc cggaaccgat gattttgacc cgtaaggagg
     2821 gaagttgaaa ctaaaaaacc agccgtgtgg gctggagttg ctatttcttt ttaataatga
     2881 gtttttctaa atttaattca ttctcacgag tttgcgtcca ata
//
LOCUS       DACCXM010000194         2838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCXM010000194 DACCXM010000000
VERSION     DACCXM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.203"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            152..841
                     /locus_tag="IVY61_002744"
     CDS             152..841
                     /locus_tag="IVY61_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7971796.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            855..2357
                     /locus_tag="IVY61_002745"
     CDS             855..2357
                     /locus_tag="IVY61_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7971797.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2370..>2838
                     /locus_tag="IVY61_002746"
     CDS             2370..>2838
                     /locus_tag="IVY61_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7971798.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKI"
BASE COUNT          915 a          392 c          558 g          973 t
ORIGIN      
        1 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
       61 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
      121 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
      181 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
      241 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
      301 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
      361 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
      421 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
      481 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
      541 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
      601 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
      661 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
      721 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
      781 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
      841 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
      901 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
      961 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     1021 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     1081 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     1141 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     1201 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     1261 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     1321 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     1381 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     1441 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     1501 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     1561 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     1621 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     1681 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     1741 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     1801 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     1861 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     1921 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     1981 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     2041 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     2101 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     2161 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     2221 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     2281 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     2341 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     2401 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     2461 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     2521 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     2581 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     2641 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     2701 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     2761 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     2821 attcatgtgg aaaaatca
//
LOCUS       DACCXM010000195         2775 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCXM010000195 DACCXM010000000
VERSION     DACCXM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.204"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(804..2144)
                     /gene="glnA"
                     /locus_tag="IVY61_002747"
     CDS             complement(804..2144)
                     /gene="glnA"
                     /locus_tag="IVY61_002747"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP7971799.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(2172..2564)
                     /locus_tag="IVY61_002748"
     CDS             complement(2172..2564)
                     /locus_tag="IVY61_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7971800.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          820 a          602 c          452 g          901 t
ORIGIN      
        1 acctaacaaa gaactacttt ttcctacttg cagcaacagt ttatatagct tatcgtagaa
       61 acaatctttt tctttcaccc ttatttacct cctttacgta aaaaacgtag tccacagttc
      121 tcggattttt ggcatatctt ttctattaaa taatatcaaa aacaaaacaa ttccactaat
      181 tttttcaaag atgacctttt tttcaacaaa agtgccttgt agggattcat tgaaggtaac
      241 gaatcacata agtaaaaaat aaggagatgc gacaaaagtc gtgcctcctt atttctaaat
      301 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccatca aacatgagaa
      361 gctgtttttg aaattatttc ttgtgattcg gagttaaatg gtaggctcac aacctctgga
      421 taaacggtat tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtagaa
      481 agccattttc acaaaatttt tcttaatact cgaaactaaa cgtctttttc aaaacctctt
      541 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagt tcagccaatt caaaaacaag
      601 aaaagctgtt tttgcttggc tattcttatt acttgttgca gggcagcttt ttcacaacct
      661 ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      721 tgaggaacta tttttccgtt agtttctcta atactcaggt caaaacgctg atttcacagc
      781 ctctaattca cggtattcct gttttaataa agttctagat attgttctct ttcccactcg
      841 gaaacagttt gtctgaatgc cgcccattcg agacgttttg cttcaacaaa atttgagaag
      901 atatgttgtc ctaatgcgtc aatcattaca ttatccttac gcaattcttt gatagcatta
      961 tggatagttg aaggtaagtc ctggatatgt gcagcttctc tttcttcctc atccattaca
     1021 taaatgttac ggtcaacagc tgctggcggt gtgatctgat tacggatacc gtctagtcct
     1081 gcttgcaaca atactgccat tgccaagtat gggtttgcag atggatcaac tgaccgcaat
     1141 tccaaacgtg tggataaacc gcgagattct ggtacacgta ctaatggcga acgattacgg
     1201 ccactccatg cgacataaac aggcgcttca taacctggaa cgagacgttt ataagaattg
     1261 actgttggat tgcaaacggc tgtgtaggca cgagcatgtt tcagcaaacc acctaagaaa
     1321 tgataagctg tttcacttag ttccaattca ccgtctttat catagaaaac atttccttca
     1381 tcattgaaca aagacatatt gcaatgcata ccagaaccat tgatcccaaa taacggtttc
     1441 ggcatgaaag tcgcgtgaag tccatgcttg cgtgcaatcg ttttgacaac gagcttgaag
     1501 gtttggatat tatcacaagc atctaccaca tctgcatatt taaagtcaat ttcatgctgt
     1561 cctggcgcta cttcatgatg tgaagcttcc acttcaaagc ctagactttc taattccaac
     1621 acgatgtcac ggcggcaatt ttcacctaaa tctgttggtg caaaatcaaa ataaccacct
     1681 ttatcattca aggtcgttgt gatcccaccg tcttcgtcta atttaaataa gaagaattct
     1741 ggttcaggtc ctaaattgaa agaggtaaaa ccaaggtcgc gcatatcttt caatgctcgt
     1801 ttcaaattcc cgcgaggatc gccggcaaat ggtgtcccat ccggattata gatatcacaa
     1861 attaaacgag caacttttcc atgagcactt tcccacggga agatcatcca agtggagaga
     1921 tctggataca aatacatatc gctttcttca attcgaacaa atccttcaat cgaagaaccg
     1981 tcaaacatca ttttattttc taatactttg tcgatctggc tcactggaac ttctacattt
     2041 ttgatcgtac ctaagatgtc tgtgaacatc aatcgtaaaa atcgtacgtt ttcttcctca
     2101 atgattcgtt tgatttcttc acctgttatc tgtttcttta ccatggtatc cctcttcctt
     2161 ttttaattta ttcagtcaaa gaattgtctt tgcaatcctt gcgactgata atgacctgtt
     2221 ttgcttaacc caccttgcgc caaaatctca tcacgaaaaa tttgtcgaac gtcatgatct
     2281 gtcaacggct tcttgttttt tgcttcttgt tttttcgcct gtgccatttc gtatacacgt
     2341 ttgatcccag ccatattcaa tccctctgaa agatagtctt taatctcaag caacatatca
     2401 atatcattca gcgaatacat acgtcggttt ccttcgcttc gttccggatg aactaaatct
     2461 tgttcttcat aatagcgaat ttgccgagca gacaaatcag tcagcttcat aactgtacca
     2521 attggaaata cggacataga tcggcgcaat tccttttctc ccatcagacg tcccctttcg
     2581 ttttataagt tggctaaagt gtaccaatta aaaaaaatat tgtcaactgc atatgttagg
     2641 ttttctaaca tgagatctct attttttata aaataacaaa aaaataagaa cacaaaaaag
     2701 gtatctagtt gattttcgta ccagatacct tttatttttt attatactgt actatccttt
     2761 attagtgttt ttatt
//
LOCUS       DACCXM010000196         2770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCXM010000196 DACCXM010000000
VERSION     DACCXM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.206"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(77..1876)
                     /locus_tag="IVY61_002749"
     CDS             complement(77..1876)
                     /locus_tag="IVY61_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7971801.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2000..2596)
                     /locus_tag="IVY61_002750"
     CDS             complement(2000..2596)
                     /locus_tag="IVY61_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7971802.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          978 a          522 c          480 g          790 t
ORIGIN      
        1 aaaaaacgca tatacctgcg gtggcaggtt atgcgttttt tgttgtataa agactttttt
       61 cattaacgat ttttgattag ccgactccca aaacaaaatt tagcagttta tctgaataag
      121 tgatttcttc atctgtttgt ttcacacttt catttagtag tcccatctca tcggttatga
      181 taccatccac accataaaac atcatgcgac tcatagcatc tgaatcgttg attgtccata
      241 catatacttc ttttccgtct tggtgggcag aattaatgaa gttacggttg atcgtactat
      301 attccattgt cagaaaatca gcaggcgtca cagggggccc aacaatattg aaaggtaaga
      361 tgtagcccac ataaaagttt ggttctgctt cttttaaatc tgaaacggtt tggtacgtca
      421 aactttgcaa aatatgatgg ttctctaaga taactttgcg gtatttatgg acaaatcgtt
      481 cgacaaggtc atccgaatct tttttagagg tcttgatctc aataagtaat ttttggttta
      541 gcttatgtgc tttttccaaa taagaatcaa aagaacaaac aggggcttgc actccgtttt
      601 cagtaactgt aagatttgtc aattctttca atgtaagatc ctgtggtgtt ccatttaccc
      661 ctgttaagtg acgaagattt gcatcatgca tcactacgaa atgaccatct tttgtttcct
      721 gaacatccat ctctacataa tcaggatgat agtctctgct tgttttttca agtgcttcta
      781 aagtattttg cgccccattt tgggtactca cacctctgtg agagacagtc actggttttg
      841 tctgaacagc tgaagtcaga taatccatat tgtaaagaca gacgccaata ccgaaaaaaa
      901 ctgcaaaaag agtcaaacca gtgtttttca gcgcagagaa acgcaagttg ggggcttgtg
      961 ggataaacca cttagggatc tcaggcaaga agccttcgtc atccatgaaa tcaacaatga
     1021 tataaaaaat ccctacggta gacatcacga tattgaataa taaaatccct tgtaaaagcg
     1081 tcatagcaaa cacagcgctg atcaatgcat agtctgggaa aaattgttca accatacttt
     1141 gtgccaaaat aacaacgata tatcctaaag aagaaaggag taaaatcgta ccgccaatca
     1201 caataaattg gcccgtgatg gccaaaagac gcgatttggt caatgaccag ctttcccgaa
     1261 tggctgctcg aaaaggacga tctcgtagaa tcatttccgg taaagcaaaa atcagtcgaa
     1321 tcccaatata gccaaggaaa atataaacga gtaaaaagga actgacgatg atccaacgat
     1381 tagcaaaaat aaaatccata atgaaagcgg ggatttttat ttttgacagt agatcagaat
     1441 taaaacttaa tccgctgatc ggtagaatca ataaaaaata agcaaggaaa aacaaaaaag
     1501 taatgggtct cactttcttc agctgaagta tcgtcaagtg taagagttgc ttcaaagaga
     1561 ttggttcttt ctttttaata aaaaaaacac tcatcaaaag aaaagtaaat tcgaaataaa
     1621 ctaacagtaa aatcgtcatc aaaactacga ttaacgagag tagaacccca ggatgttgag
     1681 aaaaaattga aggaatattg tcataagaca aatagtcaat tgcccctcgg tgtaaaataa
     1741 aacgggtcgt actgctcaat aagggtaaac aaataaataa aataaagcca tgcatcagca
     1801 agacatcacg gaaataagct tgtgttcctt ttaaaaagtc ccaagcatta cctaagctac
     1861 gtttcaaata attcatccta tcacctgctc ttcacattgt agcgcaaatc taatgaaaaa
     1921 ggaaagggtg cagtgtgttt tgcagaccct ttcccagatt taacattgtc aatcaatgac
     1981 taattttaca aaataaatat tatccaagaa gaccacttag atattgacct ccataaatca
     2041 aggccatact atataagaaa atcgacagtg gtttagcaat caaaatgatt agtgtatatc
     2101 gtttcactga catgtttgtc aatccagcca tcaaacataa cgcatcgtca ggagcaactg
     2161 gaagaaaaat agctagagca aacagcttct caaaacggtt ttgattatcc agccagccaa
     2221 tatatttgtt atatgtctta tcgctaatca tggaaagtat gaatggcttt ccataacgac
     2281 gccctagcag aaaaatgata atcgaaccaa tgcagattcc aatatagtta tagataaatc
     2341 cagcataagg gccaaatatc aggacaccag ctgcagtact gatccctcca ggaatgattg
     2401 gaataactac ttgcaggatt tggatgaaga caaaaataat cggtcctaaa atgatggaat
     2461 cacctactaa tcctcttaac gcattcaaat ctttgaaaac acctaaatta ataaaataaa
     2521 tcgtcagcgc gattgatagg cctaatccga taatggatat gaaatttatc agttttcgag
     2581 aggcagcaat attcattatt catcaactcc ttagaggttc tttgttaact atactttact
     2641 agaaaagcaa ctaactgcgc cattttttga tatgacagaa aacaaacaat tttgtaagtt
     2701 catcataagt ataaaagatt gatctcaaaa aaacatccaa ctaaagactt atttaataac
     2761 ttgttttttt
//
LOCUS       DACCXM010000197         2753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCXM010000197 DACCXM010000000
VERSION     DACCXM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.207"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(16..1035)
                     /locus_tag="IVY61_002751"
     CDS             complement(16..1035)
                     /locus_tag="IVY61_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7971803.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            complement(1046..1243)
                     /locus_tag="IVY61_002752"
     CDS             complement(1046..1243)
                     /locus_tag="IVY61_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7971804.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(1266..1559)
                     /locus_tag="IVY61_002753"
     CDS             complement(1266..1559)
                     /locus_tag="IVY61_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971805.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            complement(1596..1733)
                     /locus_tag="IVY61_002754"
     CDS             complement(1596..1733)
                     /locus_tag="IVY61_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP7971806.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            complement(1735..2181)
                     /locus_tag="IVY61_002755"
     CDS             complement(1735..2181)
                     /locus_tag="IVY61_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971807.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            complement(2184..>2753)
                     /locus_tag="IVY61_002756"
     CDS             complement(2184..>2753)
                     /locus_tag="IVY61_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971808.1"
                     /translation="DWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTE
                     KGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVYTLTKDNSQSFIDGYATF
                     IKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGMLTGPSGTSSVSKAMYVR
                     KDLGTIITNSEFAANEWFTFHGNYWVGEE"
BASE COUNT          745 a          578 c          448 g          982 t
ORIGIN      
        1 aacgaggggc tatttttatc gttgcggaat atttaaatac cagcgtttat catgaaaatc
       61 ttgtgctccg cctttagtgt tcccttctgg atcattcgtt gcccgcatca ttacatagac
      121 tttcttatta gggaagttgc gcatgttaaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacat ctggtctata tattccatca gcttttactc gagctaattc
      241 tttcccagta ttgtaatcca taatgaagat atactcgtat ttatagttag cgatgtgcca
      301 accagccaca tgtaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagggtag gatttgcagc acctgctcga gttggatcaa tgactggttt
      421 atcatctgaa gttgtcggat tttcatcggt aaatccatga gccaaatcat aggctaattt
      481 ttctttactt acgcccattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat ccctggttga ttatacggtg tgtctagtgt
      601 aagtggaata ccatatttca ttgctgaatc tctagctaat tcaacgtatg ccttgtagtt
      661 tttctcaaac gttgctttat catgtgtgtg ttgtaactca atctgcacag gactatttgc
      721 attagcatac gaaccagcac cgtactgtac ataaccaggt tgaccgactt gataaacaat
      781 tccgccgtct cccacaatat aagcagtata agcgctagtc catgaacgtt gcatatactg
      841 cgcttcattg cgtcctgttg ctgtttcatt agccgtttca tgcagtaaaa tgtactgatt
      901 atttgctact tgtgagctac cttcgtttgg gcctaaatta aattcattgt taatcgtata
      961 ggcaaaccca ttaattggca ataaaaaaag agccgttaat aggctcatcg cagtaatagt
     1021 aattttcttt ttcatttgtt tcctcctatt ttttcaaatt ataagccgac acgccagtga
     1081 taacgcctaa aaatgttgct actgcattga tagtgagtac tgtcatatct gttccattcc
     1141 atccatacgc tttgcctagt gttgcaacta atacagatgc agctggcaat actgttaaaa
     1201 ctgtccattt aatgacttga taatacttat cgggtaaaat catttcttct taactccttt
     1261 cttttttacc gagatctttc tctaaataaa gttttgattt gttgtgtgtg ttctaccaat
     1321 ttttctgtat gtgtatctaa tctttcatcg tgtttcttta gttcttcatg aatcatcaat
     1381 cgatctgatt tgctcgattc taaatcttta gtcagcaaat ctaaattgtg acttactttt
     1441 gaaagagttt cagtaatctt cgagaaagat gcagtgactg gtcttattac taataaaatc
     1501 aaagaaacaa tcgcagtgat tgatcctgcg attgttcccc attctcctaa attaatcatg
     1561 tgacaactcc tttaccttaa ataaaaacgc atcacttaag atgcgctctt atctttatta
     1621 atgatttttt ctgcttcttc gtctgtaatg catagtggaa cgaatagtcg aacttgatcg
     1681 tcagtaaaac agccccaatc atacatcatt ttcacatcgc taaaactaaa catactactc
     1741 acctcccttt gaagctggat ttagttgctc tttaatttct gcaatgtctt tgctattttg
     1801 gaacgaagca agcatcgttt ttgaattgat ttgtgctaaa ccatccgctt tttctttcaa
     1861 tgcagtattt tcctgtttaa tcgctacatc gcttagcatg agtttggcgt tgagctgttt
     1921 taaatcgccg ttctcatttt ctaacgactc atacatcgct ttgagattgt ttaaatcgtt
     1981 gtgatctagt acattcacta aaacaatcca ttggttcagt ttaggatcaa acatctgatc
     2041 agcaatcgtt agtggttccc catcagcacg aattccttcg agtggtggaa catcggtaaa
     2101 tggaacagtc ataaccatat catcaagcac ttgtccagca tactctccac ctgttcgtcc
     2161 gtatttccaa atatttttca tttttattcc tcccctaccc aataattacc atggaatgta
     2221 aaccattcat ttgcagcaaa ttcgctattc gtaataattg ttcctaagtc ttttcgcacg
     2281 tacatcgctt ttgatacact acttgttcct gacggacctg taagcatgcc atgaacaatt
     2341 tttgcgacaa attcatctgg gacttcatca tcaagtggta caccaaaagc ataagctttt
     2401 ttgaacttta ctgtaccatt tacaataact tcttttccgt gtttaataaa tgttgcatac
     2461 ccatcgataa acgattgact gttgtcttta gttaatgtat aaacagtgca atcattttca
     2521 ctgatcactt tatctcctgc tatttgaata ccatcggcaa agtttttcaa accttcaact
     2581 ctctgaggat catacacatt aacagcatca ttcaagcctt tttcagtata ttcaggtgtg
     2641 acatcccaac tgtaatcatt gggattgtta ctgtctttca agccttcacc aaagtattta
     2701 aactgactaa tattcggggt tcgtgtgtcg cctttttcga tcttgagcca gtc
//
LOCUS       DACCXM010000198         2739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCXM010000198 DACCXM010000000
VERSION     DACCXM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.208"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IVY61_002757"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IVY61_002757"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7971809.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DACCXM010000199         2707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCXM010000199 DACCXM010000000
VERSION     DACCXM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.209"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            7..1695
                     /locus_tag="IVY61_002758"
     CDS             7..1695
                     /locus_tag="IVY61_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971810.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1682..>2707
                     /locus_tag="IVY61_002759"
     CDS             1682..>2707
                     /locus_tag="IVY61_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971811.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRI"
BASE COUNT          898 a          403 c          451 g          955 t
ORIGIN      
        1 aaatctatga aaataagtaa aaaagcattt cttttagtag ttcttatctt acttagtact
       61 ttatattcag taaattttat gagaaatgct caagaaattt atacaacagg tgatttatct
      121 tttcatcttt cacgaatcaa agggttatct tctatttttg aagggccaat caactataca
      181 acatttaaca attatggaga tggactgaac tatttttatc cttttcttac aataattcct
      241 gcagtcgttt tttatggaat ttcaaataac ttaatattaa gttatgttct atatatttgg
      301 ttattaaata tttgtacgat tctgattagt ttttattatg gagaaaagtt ttttaaaaag
      361 atagatgctg cttttttatt tagttgttta tatacttttg caacttatcg aacagttgat
      421 atctattatc gatccgcaat tgcagaagca atcgctctta cgattatccc agctgtttta
      481 tattatgctt atggattgat attcaataga gaaaaaaaag tcatcgcttt atctgtcagt
      541 ataagtctac ttgtgtatac tcatgtctta actacactat tgtgtagtgc attgattgct
      601 tgtatgatta tagttcgttt gtttttaagc aagcaaataa aaaacgatat aaaatttgtg
      661 gtcgtgaatt tagtaaaggc tgtttttttg acttgtgcat taactagcta tttttggtat
      721 cctatgttgc agcagatgaa ttatcaagaa atacacagtc ccttcaaaac agatttgcag
      781 ctagaagcac tttctatttc agatagtatc attcaagcgt taaataacga tattacaaca
      841 tacactatgg ggttattagg attggttgcg ttgatttttc ctttattcat actgaaagaa
      901 atggagaaaa aagaaaaaat catctatgtt ggcgtagttt ctacatgggt atgcgtaact
      961 aatttatttc catggttttt gtttcaaaaa acaccgataa gcttactcca gtttccttgg
     1021 cgtatcttag gtatccaaat ctttttttct tcaatgatca taacgcttgt ttttatgaaa
     1081 aagactatga ataaaaaata ttcatggtta atcattggcg ctactgtggt gtttctactc
     1141 gtcacgtcta tagcagcaaa agaaaattac gcaaaagtta tccaatcaaa acacggtcat
     1201 attgttatca atgaagaaac actcccacgt tatacaacat cagatatggg aggattatac
     1261 gattacgccc caacagaagc gataaaagaa aggcctcact tagaaaaaca tgaagtaaaa
     1321 gtaggagaat cgtgggaaaa agggaagtat aaagctgcag ataataacat tacttacaca
     1381 gtggcaagca acaaaaagca aaaagtgaca gtaccagtat atgcttattt aggtacacag
     1441 gtaaaagtaa acggtaaagt cgtaaatgac tcttataaaa aacatggctt agtgaatgtg
     1501 gatgtaaatg ctggagaaaa taagatagaa attacaacaa aatatactcc aactgctctc
     1561 atggctttat ggatcagtat tctgacttat tgtttagtgg tgtattcaag ttttaaacga
     1621 tttgcaccta ataagttgaa agattctcga tatgtgacta aaaaggagat aaacaagaag
     1681 aatgaaaata aataaaaaag tagtaatatt gagtggatta attatagtta gtagtatata
     1741 ttctttcatc tttttcaaaa atacacagag tatttatacc actggagatc tttcttttca
     1801 cttatctcga atcaaggggt tatcgactat ttttaccagt ccgattaatt atgagacatt
     1861 caattatact ggatatggtg taaattattt ttatcccttt ctgacttttt ttccagctgt
     1921 aatgttatat tggatgacaa aaaatttgat tgtaagctat attatttacg tttggttatt
     1981 gaacttgtgt acaacactcg tagcttatca ttatggcgag cggtttttaa aacaaaaaaa
     2041 agcggcattt ttatttagtt gtctctatat tttctctgct tatcggacag tagacatcta
     2101 ctatcgttcc gcaattgccg aagctatcgc aataacactt gttatccctg tattatttta
     2161 tgcttatcaa atcatctctg gaaaagaaga aaagtatccg tctgtaaagc tagcattgag
     2221 tatgagtttg ctagtgtaca gccatgtact atcaacatta atgagtacag cattaattat
     2281 catttttatt tttattcgtt tggtttctaa aggtttcaag aatgcagatt ttattgctat
     2341 atttaaaaaa ttatttagtg cggcaggaat gacattggtt ctgacgagtg ctttttggta
     2401 tccgatgttt gaacaaatgc tctaccaaaa aatcaacaaa cctagcgtga caaatttata
     2461 tgctcatgca tctaatgtgt tcgatagtct gactgaagct atgaacaacg acttaactac
     2521 ttactcaatg ggactagttg gtctgttatc tttatgtatt cccctgattt tatttaaaaa
     2581 attaacaaac atagaaaaaa agatttatta tggtacgtgt ttgacttggc tagctacaac
     2641 tagtttggtg ccatggtatc ttctgcaaaa tacgccagcc aaattgttac aatttccttg
     2701 gcggatc
//
LOCUS       DACCXM010000200         2705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCXM010000200 DACCXM010000000
VERSION     DACCXM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.210"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            42..1520
                     /locus_tag="IVY61_002760"
     CDS             42..1520
                     /locus_tag="IVY61_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP7971812.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(1588..2679)
                     /locus_tag="IVY61_002761"
     CDS             complement(1588..2679)
                     /locus_tag="IVY61_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7971813.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT          944 a          492 c          440 g          829 t
ORIGIN      
        1 aaattgtttg ttattattag caacaaaaaa aggagtgcat catgaaaaag ttatctcaaa
       61 aacgtttagc tcaaatcatt attgattcta gaaaatctct gaaaatgaca caacaacaac
      121 ttgcagtagc tactggtatc aaccgtgcct taattagtcg attagaatct ctcgatttta
      181 tcccgtcaat acaacaatta gaatcattag cagaagttct aaattttgaa ccaacagatc
      241 tatttataaa tgaatcaaat aaatcaaaca gaacaaaaca tcagtcgaag aatattgctg
      301 tagccggtac tggttacgta ggtctttcca ttgctactct tcttgcccag cacaatcagg
      361 taacagcagt agacattctt cctgaaaaag taaaacaaat taacaataag aaatctccaa
      421 tacaagacga ttacattgaa aaatatctag ctgaaaaaaa tcttaattta attgcaacga
      481 ctgatggtgc agccgcctat agagaagccg attttgttgt cattgctgct ccaacaaatt
      541 atgatagtaa acaaaattat tttgatactt ctgctgtaga gtcagtaatc aaattggtgt
      601 tagaagtcaa tccaaaggca ataatggtca tcaagtcgac ggtacccgta ggctataccg
      661 aaagtattcg taaaaaatac aatactaaaa atattctatt cagcccagaa tttctcagag
      721 aatctaaggc attatatgac aatctatatc cttcgcgaat tattgtcggc acggaccaaa
      781 ctgacaaaac tttacgagaa gcctctcaca cttttgcaga tttactccaa gaaggagcaa
      841 tcaaaaaaga tatcgaaact cttttcatgg gattcacaga agctgaagca gttaaattat
      901 tcgctaatac ttaccttgca cttcgagtat cttacttcaa tgagctggat acttatgcag
      961 aaatgaaagg actaaataca caagaaatca ttcacggggt ttgcttagat ccaagaattg
     1021 gcacacatta taataatcca tcttttggat atggaggcta ttgtttaccg aaagatacaa
     1081 aacaacttct cactaactat aacgatgttc ctcagaatat gataagtgca attgtcgata
     1141 gtaatcgtac aagaaaagac tttatagcag atcgagtgtt agaacttgct ggcacgtatg
     1201 aggctaacag ttcgtggaat gcagaaaaag aaaaagaggt agtcataggt gtataccgtc
     1261 ttacaatgaa gagtaattca gacaacttcc gtcagtcatc tattcaagga gttatgaagc
     1321 gaattaaagc aaagggggca actattgtaa tttatgaacc aacacttgaa aataacacta
     1381 cattttttgg ttcaaaagta ataaataatt tagatgattt taaaaatatc agtcacagta
     1441 ttattgctaa tcgttacgat agtaatcttg atgatgtaaa agaaaaagta tataccagag
     1501 atttatttca acgagattaa aaaataacac ctcagatttg tagtgtctga ggtgtttcta
     1561 taaactttta taagtaaaaa aatacaatta tacttcataa aaagacttat accactgcgc
     1621 aaatttacga agcccgtcac gcaaacttgt acttggttta aattcaaaat ctcgttctaa
     1681 cgcactagta tctgcataag taactggcac atctcctggt tgcattggaa ctagttcggt
     1741 atgagattca aagtcgtaat cttctggtag cacccctgct gcaattagct cttgttgtag
     1801 aatatctaca aaatctagta ggttttccgg cgtattgtta ccaatattat aaatatgata
     1861 tggtgggatt ggtagcccat cttccccatc aattttctct ggagcgcact gcataaccct
     1921 ctttactcct tcaacaatat catctatata ggtaaagtca cgcttgcaat tcccataatt
     1981 gaatatttgg atattatcac cagcacgaag tttatcagta aaaccaaaat aagccatatc
     2041 tggacgccca gcaggaccat atactgtaaa gaaacgtaac cctgtagacg gaatattgta
     2101 aagtttagaa tatgcatgtg ccattgattc attacttttc ttagttgctg catacaacga
     2161 tacttgattg tctactttat cctctgtaga ataaggaatt ttcttattag taccataaac
     2221 ggaagacgat gaggcataca ctaaatgttc cacaccagat tctccattat catatgaatg
     2281 acgacaagct tcaagaatat tatagaatcc tattatgtta gattcgatat acatatctgg
     2341 attagtaatt gaataacgga ctcctgcctg agctgctaaa ttcaccacga tacttggttt
     2401 ataattatca aagatgaaat caattgttgc tttatcagca atagaacctt tgataaaatt
     2461 ccaagtagat cctggatggt tctttgcctc cgtttctatc tctgataatc tatagttctt
     2521 aatagagaca tcatagtaat cattcatatt gtcaatgcca atgattttta ttgatgactg
     2581 agtacgtaat aactcaagaa ccaaatttga tccaataaaa ccggctgcgc cagttattaa
     2641 tattgttcta tttgataagg acgtgtttaa ttccagcatt ttttagcact cactttcttt
     2701 ttttc
//
LOCUS       DACCXM010000201         2683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCXM010000201 DACCXM010000000
VERSION     DACCXM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.211"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            21..272
                     /locus_tag="IVY61_002762"
     CDS             21..272
                     /locus_tag="IVY61_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP7971814.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            374..2371
                     /gene="tkt"
                     /locus_tag="IVY61_002763"
     CDS             374..2371
                     /gene="tkt"
                     /locus_tag="IVY61_002763"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP7971815.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2549..>2683)
                     /locus_tag="IVY61_002764"
     CDS             complement(2549..>2683)
                     /locus_tag="IVY61_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7971816.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          890 a          485 c          605 g          703 t
ORIGIN      
        1 gagtaaaagg agggctgagc atgctgtcac ctgaaaaaat agagcggatc aatgaacttg
       61 cacgtaaaaa gaaggaaaat ggtctctcaa aagccgaaga acaagaacaa atcaacttgc
      121 gcaaagagta tttggaagct tttcgttccg gtatgcgtca tcatatcgaa ggaatgaaag
      181 tcgtcgatcc tgagggcaat gacgtgaccc cggaaaaatt aaaagaaatc caaaagaaaa
      241 aaggcttaca caaccgaaat gacaactttt agtaaaaaat actgaaaaag attgcttttt
      301 cgtgtgacat cttatagaat gaaagtgtaa gaaataaagg taaaaaaaac ctaaattaat
      361 aggaggggtt attttgtttg acaaaattga ccaacttggc gttaacacaa ttcgtacatt
      421 aagtattgaa gcagtccaaa aagcaaactc aggacatcct ggattgccga tgggcgcagc
      481 acctatggca tatgcgttat ggacaaaaca cctgaaagta aatccaacga catcaaaaaa
      541 ctgggctgac cgcgaccgtt tcgttctttc ggcaggacat gggtcagcaa tgttgtatag
      601 cttgcttcat ttagctggct atcaagtgac aatcgatgat ttgaaacaat tccgtcaatg
      661 ggattctaaa acaccaggac atcctgaagt acatcataca gatggtgtag aagccactac
      721 agggccatta ggacaaggga ttgcaatggc tgtcggtatg gcaatggcag aagcgcacct
      781 tgcagcaacg tataacaaag aaaactttaa tgtgatggat cactatacgt atgcaatatg
      841 cggtgacgga gacttgatgg aaggggtatc tcaagaagca agttcgatgg caggtcatat
      901 gaaactaggg aaattgatcg ttttatacga ttcaaacgat atttcactag acggtccaac
      961 ttcaaaagct ttcacagaaa acgttggtgc tcgctatgaa gcatatggct ggcaacatat
     1021 cttagtgaaa gacggaaatg acctagaagc aatctctaaa gcgattgaag aagcaaaagc
     1081 tgaaacagac aaaccaacat tgatcgaagt aaaaacagtc atcggctatg gtgctcctaa
     1141 agaaggaaca tctgctgttc atggtgctcc tcttggtgaa gatggcatca agatggctaa
     1201 agaagtttat ggttgggagt atccagattt cactgttcca gaagaagtag ctgcccgctt
     1261 ccatcaaacg atgatagaag aaggacaaaa agctgaagat gcatggaatg aaatgtttgc
     1321 caattataaa aaagcttatc ctgaattagc acaacaattt gaagatgctt ttgatggcaa
     1381 actaccagaa aactgggatg cagaattgcc aacttatgaa gttggaagca gtcaagcaag
     1441 ccgtgtttca agtaaagaag tcatccaaga attatcgaaa gctataccaa gcttctgggg
     1501 tggttcagcg gacttgtcag gttccaataa tacaatggtg gctgcagata aagatttcac
     1561 tcctgaacat tatgaaggac gcaacatctg gttcggtgtc cgcgaatttg caatggcttc
     1621 ggctatgaat ggtatccaac ttcatggcgg aactcgtatc tacggtggaa cattctttgt
     1681 ctttgtcgat tatctaagac cagcagttcg tttagcagca atccaacata ctcctgttgt
     1741 ttacgtctta acacatgact cggttgctgt cggagaagac ggaccaacgc acgaaccgat
     1801 tgaacaacta gcatctgttc gctgcatgcc tggtgttcaa gtgattcgtc cggcagacgg
     1861 aaatgaaaca cgtgctgctt ggaaagtagc gatggagaca acagatgcgc ctactatcct
     1921 tgtattgagt cgtcaaaacc ttccagtatt gccttctaca aaagaagtag cagatgaaat
     1981 ggtgaaaaaa ggcgcgtatg ttctttctcc atcacaagga gaaacaccag aaggtatttt
     2041 gattgcaaca ggttctgaag ttgatttggc tgtgaaagct caaaaagaat tggctgaaaa
     2101 aggcaaagat gtttctgttg tttctatgcc aagctttgac ctctttgaaa aacaatcttc
     2161 tgaatataaa gaaagtgtac ttcctaaatc agtgaagaaa cgtgtggcta tcgaagctgc
     2221 tgcatcattt ggatgggaac gctacgtagg aacagagggc gcaacgatta caatcgatca
     2281 tttcggtgca tctgcccctg gcactaaaat tttagaagaa tttggattta cagtagaaaa
     2341 tgtcgtgaat acgtacaatc aactatcata gatccaagca attgtttgac tgataagaac
     2401 agctgtctgc aagttggaga cagctgttct ttatgtgtta gaagaataaa tcaatattac
     2461 ttttcgtttt cggctatgtt acatttaaat gacaaaacaa agaattcttc ggattagttt
     2521 gtgtttcttg agaatattat ttgtcaaatt aagcgagaca aaactttcca tttacgtaac
     2581 tcaaaaaaac ttctaatggt gtttggtaat ttagtgattt tctagggata tggtttcttt
     2641 tggacgcaac ggatgagatg aatccttgat tgacttggtt gaa
//
LOCUS       DACCXM010000202         2681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCXM010000202 DACCXM010000000
VERSION     DACCXM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.212"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(936..1454)
                     /locus_tag="IVY61_002765"
     CDS             complement(936..1454)
                     /locus_tag="IVY61_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP7971817.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2572..>2681
                     /locus_tag="IVY61_002766"
     CDS             2572..>2681
                     /locus_tag="IVY61_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7971818.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          889 a          484 c          393 g          915 t
ORIGIN      
        1 aatttacccc tatactacta gtttgtaagt tagattaata aaaatactaa agttatttgt
       61 tcttttatta aatacaccta caacttataa attgatggta tttagataac acgaaagaat
      121 aaaaataaca gctgatagta aagtttgttg acgattgcta ctcaatcgaa agtgagttag
      181 tgcgtatgaa cttgcttctg aaaacttatg aaatgaaaga ccttttacat cttgcagaaa
      241 tcgttactgg gattaaacta agcaataaaa actaatgttc ccctcattct ttgaacttct
      301 actatttgat ttatttattc tgataaatga cgatttcacc ttcaggatct tcaaatcccg
      361 tatagaattt atttaagatc tcttcagtat cagtaatagg ataggcgata ggccacatac
      421 cctttgatgg ttagaataaa ctgctcaaaa tctgtatcgt atgttggctt tagcatatta
      481 acaaaggcta tctccaaatt ataaaccata agcgttgaat cgttcttaac aatttcaatt
      541 cttcctaacc caaagtcaga agatataatt accggacttg ccttaccctt catgtgggtt
      601 gttgagattc catatttaaa gggaattgtc ggtactatcg gcggtctgaa aaacatccca
      661 aaaataaaaa aatctttccc ctgctagtct cctaagtccc tttgtagcgg atatggcacc
      721 ataatctcat tttatcgaag tgagtcatgg tttatctgtt ggccaaccaa aattgataca
      781 atctaacgat gcaccctcta cggcattcta ggggagtgca cttgcattca cccaataaaa
      841 accggcagtt agcaaagaac ttatgtagtc gtatgttctt actgttaaca tacaagaggt
      901 ctcctttcca caattgctta gcagtaaacg ggaatttaca aagctaatct cttccatttt
      961 cttatctttg ttctaaaagt cccaaatgga gcaactgtat ttacatgaat aaatttatat
     1021 acttcccata cagccgtttt ggttgcctcg tctgcccatt ttcttttgtg tgcagtgaat
     1081 agatcttcat cactcattga atcaatcatc atgttaatat catcaatatt acgattgaga
     1141 atttcctcta attctttcaa tggtagatgt gcataggtat ttgtaaacca cttatataac
     1201 tctcctaact gattccattt aaattgatca gaaggcgttt taacagataa tccatttctt
     1261 tcatcatttt cccattttag aattaaattc gtccaaccaa cttgatatgc taaattttca
     1321 gcaggtgttc tatccacttc ctcaactctt ttatccttca attcttcagg aatagtttca
     1381 aattctaaaa tatatttagt ataagtccta tttatttctt tttttaattc ttctttattt
     1441 ttatatgttc tcattatatt acctccataa attcctaatt gtctccttta attcatctat
     1501 aatttcatcc acgatatttt aatccgttct acaatgcata tctgatatgc attgactgtc
     1561 taactcatat gtgaatgatt tataaaatat cccaacgtct catctaaaga aatgttgatc
     1621 gtcagcaagg agctaaaaaa tacgaatata tttcttttgt agtaaaatcg gactggtttg
     1681 catatcattt tcttcgtaaa tatcaaaatc atgttaacta ctatcaatta ctaataaaaa
     1741 ctaccacttt aagcgttaag aactgttaag tttagttaag tgaggatagt tcacaaaatc
     1801 cattgttaaa cttcacgaaa gtggtaaaac tcaaaatgct ttatatagga ttacagcatc
     1861 gctctttctt cgattgcgcg ttgggtaaaa aatattatga agtaaaaatt gacgatgaca
     1921 cgttccttac ggctcatcag atgaaacagc tacaaaaacg aaatgatcaa ctcgaagaaa
     1981 atttaattct aaaaaagcga ttgccatatt cacgcctcgc tcgacgaact attaaaggtc
     2041 aagatttacc tgaagatcat cctttttagg aaattgaagc tttttatgtt gcacaatgca
     2101 tttataatac tacaatgatg ttttcaccag atattcttct agggtgcggt gtaatgaaac
     2161 agatacactt gcaaaagaaa attcaccgcg aatttactaa aattatgaat aattatgtta
     2221 atcttccgaa atttttttcc aaaattaagg gatcaagctg gtgttataga ttgtttaagg
     2281 ttggcaaaaa aataacttct ttttaaacaa ttatagaaaa ccgatactct gttaagttgg
     2341 ttgacacggt gtcgattttt atgactttct tattctgaaa cttcactaac ggacagtacc
     2401 taaaaagtct atagtatgga cgtttacgag tactatttta tcaaaaatgc tgctcatttc
     2461 aacttatatg ttttagtgaa ctacccccac ttagcttgga taggctacaa ttaactagac
     2521 agaaaaatta aggtgtgtag actagaagaa aacataccag gaggaatttt tatgtctaag
     2581 agaacacgaa gaactttttc acaagaattc aagcaacaaa tcgtcaatct ttacttagct
     2641 ggaaagccac gtgtagaaat cattcgagaa tatgaactaa c
//
LOCUS       DACCXM010000203         2650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCXM010000203 DACCXM010000000
VERSION     DACCXM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.213"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            13..1020
                     /locus_tag="IVY61_002767"
     CDS             13..1020
                     /locus_tag="IVY61_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7971819.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1032..2036
                     /gene="neuB"
                     /locus_tag="IVY61_002768"
     CDS             1032..2036
                     /gene="neuB"
                     /locus_tag="IVY61_002768"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP7971820.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2033..>2650
                     /gene="neuC"
                     /locus_tag="IVY61_002769"
     CDS             2033..>2650
                     /gene="neuC"
                     /locus_tag="IVY61_002769"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP7971821.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQ"
BASE COUNT          979 a          338 c          525 g          808 t
ORIGIN      
        1 gaggagatat aagtgtttaa aggaaaaaca atattagtaa ctggcggaac tggttccatt
       61 ggaagtgaaa ttgttcgtca gcttttaaaa tatgaaccaa aagctatcag aatttatagt
      121 agaggtgaag aaaaacaatt ttatatgcaa caagagctct caaatcacac taatttgaga
      181 tttttaattg gtgatatcag agactctcaa aggattgatt atgcatgtcg tgatgtggat
      241 attatttttc atgctgccgc aatgaaacac gttccagcaa gtgaatataa tcctatggaa
      301 gctgtaaaaa caaatgtgat gggaactcaa aatgtaatag atgcagcgat caaaaataat
      361 gtaaaacatt ttgtaggtat cagtacagat aaagtagtga gtcctactaa caccatgggt
      421 gcgacaaagt tattgagtga aaaattaata atagctgcca atcagtataa aggaaaaaca
      481 gaaacaatat tttcttgtgt aaggtttgga aatgtcatgg ggtcaagtgg atctgttatt
      541 cctcttttta gtaaacaaat aaaaaacaat aaagatatta ctttgacaga tgttgatatg
      601 acacgattta tgatgtcaat accggatgct gcaagattag ttctgtctgc tgctatgaat
      661 tcaaaatttg gagaaacata tgtcttaaaa atgccggcaa tcaatatttt aactttagct
      721 caatcattaa taaaaatata taatgataca catgatgaaa aatatagagg tgaaataaaa
      781 gttgtaggta tcaggccagg agaaaaatta tatgaagagc taatgactac agacgaaatt
      841 gaacgctcat atgaaaatga taaaatgtat atcattccac cacactatca tgctactgac
      901 gaactgtaca aagattatga aaaaattaaa atcactgcat actcttcaga tgcaacagaa
      961 ttaatgagtg gacaggaatt tattagaatc gcaaaagaga ataatgtcgt gacttggtaa
     1021 ggagttttat gatgaaatca gaagtatatg taattgcaga aattggtgta aaccataatg
     1081 gaagtctaga tttagcttta gaaagtattg ataaagctaa agaatgtgga gctaacgcgg
     1141 ttaaatttca aacatttaaa acgaataagc tagtttccaa aaaggctcca atggcaaaat
     1201 atcagaagga taatcttaaa gaggacagtt ctcaatttga aatgctaaaa aaattagaat
     1261 tatctcaatc tgattttatt agaataaaag attattgtat acaaaataaa attgattttt
     1321 tgtctactcc ttttgatgaa gaaagtgcag actttctaaa acaaattggt gtacaaggat
     1381 ttaaaattgg ctcaggagat ttaacaaatt taccttttct tagaaaaata gatatgtatg
     1441 ggttgccagt tttattatct acaggtatgg ctgatattca tgaggtcagt gaggctgtag
     1501 attgtttcat agatagtcca gttactattt tacattgtac ttcaaattat ccggctagtt
     1561 ctgaagatat taatttattg gcgattaaca cattaaaaga aatgttcggg atacccgttg
     1621 ggtactccga tcattcattg ggatatgatg ttggtatttg tgctgttgcg atgggtgcta
     1681 aggtaattga gaaacatttt accttggata aggaactacc tggtccagat cataaagctt
     1741 ctttagatcc tcaggaattt gcagattttg taaagcatat tagaaacact gaaatatttt
     1801 taggtgatgg tgttaagcga gcaatgcctt ctgaacttag tacaaaagaa gtatcaagaa
     1861 aaagcctagt ggtcactgag aatttaaatg tgggagatat tcttacagag aaatctctta
     1921 caataaaacg tcctggaaat ggaatagctc ctaagttttt atatcaatat attggcaaaa
     1981 aagttaaaag accaataagt gcagatagtg ttctgcttga ggaagatgtt gaatgaaaaa
     2041 aatacttata gtaagtggaa cacgagctga ttatggaata tataaacctg ttgctgagaa
     2101 tttactacaa gataattggg atgtatcatt tgttgtttct ggaatgcatt tatcccataa
     2161 atatggattt actaaagaat tgatcttagc tgacggattt agaattagtg gagaagttca
     2221 aagtttgttt ttagagaaca ataagggaaa catggctcgg tctgtggcat tagagataaa
     2281 tggtttcaca aatatatttg aacaaactag ccctgatttt gtattattac taggggatag
     2341 aggagaaatg ttggcagctg cgatagcttg tttgtatctt ggaatcaaaa cagcacatat
     2401 tcacggaggc gaagtgtctg gtactgtaga tgaatcaata agacatgcca taacgaaaat
     2461 agcatctgtt cattttgctg ctacggaaaa aagcaaagag cgtttaataa aaatggggga
     2521 agattcttgg agagtttttc ccgtcggagc tcctagaata gatactattt taaataatag
     2581 attacctagt tttagagata taaaagaaaa atatcatcta aaatttgaaa agaaaaaata
     2641 cgttttgcaa
//
LOCUS       DACCXM010000204         2622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCXM010000204 DACCXM010000000
VERSION     DACCXM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.214"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..222
                     /locus_tag="IVY61_002770"
     CDS             <1..222
                     /locus_tag="IVY61_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7971822.1"
                     /translation="TEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIR
                     GLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            403..1362
                     /locus_tag="IVY61_002771"
     CDS             403..1362
                     /locus_tag="IVY61_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7971823.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMRGKRKPNGHQEKRGKQQYQRSIHDRSDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(1448..2542)
                     /locus_tag="IVY61_002772"
     CDS             complement(1448..2542)
                     /locus_tag="IVY61_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7971824.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          860 a          521 c          435 g          806 t
ORIGIN      
        1 acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca tcgtccagta
       61 aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat taaaggcatg
      121 gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt cgggttttcg
      181 gtctgtactg aaatcaaggt attattggga atcccagctt aaatcataga taccgtaagg
      241 gattttattc tttatttaaa actttgcaac agaaccaccc gcactatgcc attacattta
      301 tatctacgcg aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg gtacactcaa
      361 attgtatttc cgacgaaaga aaacaaagga gtgtaaccat aaatgaccta caaacatctt
      421 accatagacg aactgacaat gatagaatca tattatcttc aacataataa gccggttgaa
      481 atcgctaacc gaatgggacg tgctatacaa actatttata atgtagtcaa taagttcaag
      541 caaggcaaga ctgctcttga ttattggcac cagtataaag aaaataagaa aaaatgtggt
      601 agaaaagtca ttcaattacc tgctcatgaa gtagattaca ttaaagagaa agtcactctt
      661 ggttggacgc ctgacgtcat tatcgggcga aaagaaaggt ctgtttcatg cggtatgaga
      721 acactttatc gtttattttc taaaggaata tttgatattg acacactacc gatgagaggt
      781 aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac aacagtatca gcgctcaatc
      841 catgatcgat ctgataatta tcctgatttc aattctgagt ttggtcacct tgaaggtgat
      901 acgatcgttg gcattcatca taaaagtgcc gtcattactt tagttgaaag attatctaaa
      961 gtcattatca cgattaaacc caacggccgt aaggcattag atattgaaac tgcccttaat
     1021 caatggtttt ctcgcttccc taaaaacttc tttaaatcta ttacgtttga ctgtggaaaa
     1081 gaattttcta actggaaagc cattagtaac caacatgata ttgatatata ttttgcggac
     1141 cctggaacac cttctcaacg cccattaaac gagaattcta acgggattct gcgtcgtaat
     1201 ggactgccga aatcaatgga ttttagaaaa gtgaatcaga catttatttc cagtgtcagc
     1261 aatcaacgta atcatattcc aagaaaatca ttgaattaca gaacaccaat cgaaatattt
     1321 ttgagctatg tacaagaagc attttattct agcttaattt gacaaatcat aacctataaa
     1381 aaaacaaaat aaaaaagcag aaataactgg ctatttctag cggattattt ctgcaataac
     1441 taattatcta ttttttattt tcaattaatt taaagcttga ccatggtttc aaaaagtcaa
     1501 ggaggaataa ttcgtcatcg gagattcttc ctacaacatt gacacgtccg tcgtttttca
     1561 tatccttcaa ggcgatttgt gtttctcctt tgtattgacc gtacccttca ttgtcaatca
     1621 gtacatcccc atgatgcata tctcgcgtat tatgaggcgg gaaatcttta tctttgtagt
     1681 atacacgagt catcgttgaa cgaaggatat attctgagcg atcccctcga taactatgaa
     1741 ggttatcaaa caagcagatt cgttcgtttt ctgtaatacc ttcttccgta tctactttca
     1801 atgttggata atccgcgtta aatgcttctg ccatttcttt caattcttct tcacttgcgt
     1861 acgcatttcc aatagaaata tcatcaatca atcctgtaag gattaaatgc ttcacttgtg
     1921 tcgcaatttc aagatctcgg tggtcttcca gcgtgcacaa gccatcttgc gtaggccaag
     1981 ggccaaactc agcagattgc gaattgacga atgccatcgt attcaaatta tattttctga
     2041 atttttctga acaaaaaaca aagtgttcgt atcctaaacc agagtatcga tgaggataaa
     2101 aattatgaga acctaataaa ttatccgtgt ttggcgaata actcatgatg ttgtcaacgt
     2161 aattcgtgcc cgaactcata ttgatctcga ttttgatacc aaaaggattg cgcgtcattt
     2221 ttgcttcttc tgctcctgtg aatccgatat ctaagcggac cccgtaggca cccatcttat
     2281 ggaaaaaaga taagtcatcg taagaaatct ccaactgttc aaataacgct ggattgatat
     2341 caaccattac ttccatgcct aggctattcg cgtaatcaac tacttcttta aattctgaaa
     2401 gaactttttc tttatcatca ttgatctgca gcaaactggt aaaaacccgt ttatacccat
     2461 acttatgagc taaatccaag tacgctttgt ccttttcaaa tgttgaccgt tctggataaa
     2521 tcgagattcc taattttccc atattaccat ttcctcctgc atcttttcgt ttttgtatcc
     2581 acttacatta tagcaaacac aaagtaaatg gtctacacct tt
//
LOCUS       DACCXM010000205         2596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCXM010000205 DACCXM010000000
VERSION     DACCXM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.215"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            16..1467
                     /locus_tag="IVY61_002773"
     CDS             16..1467
                     /locus_tag="IVY61_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7971825.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1816..2448
                     /locus_tag="IVY61_002774"
     CDS             1816..2448
                     /locus_tag="IVY61_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP7971826.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          968 a          376 c          513 g          739 t
ORIGIN      
        1 agagaggtaa atcaaatgaa aaaatcttta aaacttgcat cagcagttat attaatgtcc
       61 caactcctta tagcaacacc tttccatgtg ttggcaagcg aaaattcaac atacaaaatt
      121 gcaaaagaaa gaaccgagac gactgaaatg agtaattccg atgtaacagc agagagtagt
      181 gaacttttga caacttcatc aagtgaaagt agtacgactt caaatgccga gtcttcaaca
      241 acttcatcaa gtgaaagtag tacgacttca aatgccgagt cttcaacaac ttcatcaagt
      301 gaaagtagta cgacttcaaa tgccgagtct tcaacaactt cgtcaagtga aagtagtacg
      361 atttcaagta ccgaatcttc aacaacttca agtagtacaa gtgaaataac ttcttcgaaa
      421 gtgacaaaac caaaccatat agaatcttca aaaaaggaag cgccaaaaac ggaaggacaa
      481 acaaaaactt tttcgacacc acagcaaata gaatcaacag aatctttcca atcacaacca
      541 atactatctg gtaatgaaga aattcatttt gacaaaaatg aatctgtaga aaattttatt
      601 atgaaaattg gtgagtctgc gagaaaaatt ggtcagaaat atgatttata tgcttctgtt
      661 atgattgcac aagcgatctt agaatctgcc agtggtcaaa gccagttagc tcaggcaccg
      721 aattacaatt tatttgggat aaaaggaact tataatggaa actttgtcat tatggtaaca
      781 aacgaagatt tgggtaatgg aacgctttat acgacccaat caaaatttag agtgtatgaa
      841 aactatgagg aaagctttga agattatgcc aaattattaa ctaagggaat tagtggaaat
      901 aaagattttt atgcaggagc attaaaagct aatagtaaaa catatcgaga agcaactaaa
      961 ttccttacag gacggtatgc aacagataca caatactatt tgaagctaaa tgaattaatt
     1021 aaaacctacg atttaaccaa ctatgataaa gaagttaaag gtccacaaat gagtaagact
     1081 ggataccaag tgcctattca taattattca ataaccagtt tgtttggaaa tcgtggaaat
     1141 gaatttcacc gaggacttga tatggcagct acacagggtg agccgattca tgcagttaaa
     1201 gctggtaagg ttgttaaagc agagtttcac tactcttggg gaaattacgt ggtgatacaa
     1261 cacgaagatg gatcaaccgc gttatatgct caccagcaac aatacctagt aaaaactgga
     1321 gataatgttt tgcaaggaca aataattggc tatgtaggaa gtactgggaa tagtactggc
     1381 ccacaccttc atttggaaat ttgcaaagat agtagtcttt ctcagagcat gttagttgat
     1441 ccgaaaacct tattattcgg agactaaaaa aggtaatcaa tctgacaaag taacaagaaa
     1501 atggttggat tcttagacct ttgatcagga tattgagact ggaattgata tctcgattac
     1561 gatgggtttg acaaataaaa caagtccatt atcgaatatc gagtgtatta ttgccgtctt
     1621 tatgctcata ttttgagcag gtttcactag atgaaaacaa tttatctatc ttgaggattt
     1681 cgtgtacgta tcgttcagtc ttgtattgta aattagtcat aaaatcagac taagaaaaat
     1741 tggaaatatt tttttctgat ttattaaaag ttaattgaaa ttatatggta cttctttctt
     1801 gggagggtag gtgcagtgaa ggaaagatat ggtcacctta ctgttgttga ggagattact
     1861 cataatggat ataaaaaata tcgttgccta tgtgactgtg ggaatgaaac gattgtatat
     1921 cgatcaaact tactatcagg tagaaccaaa agttgtggat gtggaaatta taaaaatagg
     1981 cataaatata aaaatttgac aaataaaact tttggtaagt tagttgcaat caagccaact
     2041 aaactgcgaa gtgaaggaac tgttgtttgg gaatgtcaat gtctgtgtgg gagaaaaaag
     2101 ttagtatcgg caaagcgact tctaaaaggt gatgtaaaaa gctgtggatg tttgaaatca
     2161 aaaaagcatc acattgaaaa taaaaaattc ggattactta ctgcaaagta tcctttggac
     2221 aataactgtt catggaaaac aaaatgggtt tgtcattgtg agtgtggcag tatgtgtgag
     2281 ataagttata gtaacttatt ttttggacat actcaaagtt gtggatgttt aaaaaggcaa
     2341 gaaacaagga catgtgttga agggacagta atagagtgtc taacgtcaaa attaccgaaa
     2401 aataatacaa gtggtgttaa gggagtttat cgttctaaag agaaatagat tgcgtatata
     2461 actttaaaaa aaaagaggta ctatttagga tcgtatgatt ctattaagag tgcagctcaa
     2521 gctagaaaag atgctgaaaa taaattgttt caacctttga ttgacaaata tcgaggtaat
     2581 cattaattat tgtgga
//
LOCUS       DACCXM010000206         2595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCXM010000206 DACCXM010000000
VERSION     DACCXM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.216"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1350
                     /locus_tag="IVY61_002775"
     CDS             <1..1350
                     /locus_tag="IVY61_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7971827.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>2595
                     /locus_tag="IVY61_002776"
     CDS             1484..>2595
                     /locus_tag="IVY61_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7971828.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRK"
BASE COUNT         1018 a          360 c          514 g          703 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaa
//
LOCUS       DACCXM010000207         2552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCXM010000207 DACCXM010000000
VERSION     DACCXM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.217"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..508
                     /locus_tag="IVY61_002777"
     CDS             <1..508
                     /locus_tag="IVY61_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP7971829.1"
                     /translation="LENIEKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYL
                     KTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKN
                     IINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIK
                     WIQKQIAY"
     gene            481..1158
                     /locus_tag="IVY61_002778"
     CDS             481..1158
                     /locus_tag="IVY61_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7971830.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1148..2290
                     /locus_tag="IVY61_002779"
     CDS             1148..2290
                     /locus_tag="IVY61_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP7971831.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2303..>2552
                     /locus_tag="IVY61_002780"
     CDS             2303..>2552
                     /locus_tag="IVY61_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7971832.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALN"
BASE COUNT          952 a          334 c          500 g          766 t
ORIGIN      
        1 actagaaaat atagaaaaac aaattttagc tttgatcaat tcaatggaac aacaatctca
       61 accaattatt tgcattttac ctaatagtga tgctggtagc gaaattatca gaaaaatgta
      121 ccagaaatat ttaaaaacgg atattattta tattacgaat ttagagccgg aggaatacct
      181 gactatatta aaagagtgca aatttatgat cggaaattct tcttctggga taattgaagc
      241 tgcatcattc aaaataccag taataaattt aggaaccaga caaaatggac gagaacaatc
      301 taaaaatatt ataaatgcaa atctaaatga aaatgaaatt gaaactgctt tgaataaaat
      361 acaagatcct cagtttataa aaagcttgaa aaatgttaag aacatctacg gtgacggcaa
      421 cagttctaga agaataacaa aaacattgaa tcaaatattg aatgatgggg aagatattaa
      481 gtggattcaa aaacaaatag cttattaaaa tatactagtg atccatacat tattattggg
      541 gctggcggac acgcaaaagt cattattgaa accttaagat tagaaaataa gttaatatat
      601 ggattttttg atgataagca aaataaaatg caagatctga aatatcttgg gaaaataaac
      661 gaattgaata gctgggcgga tagcaaaaaa aaaatctata aatttattgt agctattggt
      721 gactgtgtag taagaaaaaa gattataaaa aaattgaata tgccaagtga gcaatatgga
      781 atagctgttc atccaagtgc tatagtggca aaagaaattt caattggtta tggaactgtt
      841 atttttgcga atgctgtaat taacccagat gctgtaattg gagagcatgc tataataaac
      901 acaggtagca ttgttgagca tgattgtaga attaataact atgttcattt atctccaggt
      961 gtatgcttag cgggtggagt acatgtaggt gtaggaactc aaataggaat tggttctcaa
     1021 tgtatccaga tgaagaaaat tggatcttgg tgtatgatag gcgcagggag cactattgtt
     1081 aaggatattc cctcacattc cttagcttat ggcaatccag ctaaaattaa gaaagaagga
     1141 attaattttg aattctagaa ttttattatc aagtcctcat atgggaggaa cagaagaaaa
     1201 atatgttgct aaggctttta aagataattg gatagcgcca ttaggtgaaa atgtggataa
     1261 atttgaagaa agtattacaa aatatactgg ttctaagggc ggagcttctg cgttaagttc
     1321 aggtacagct gccatacatc ttgccttaag actgcttaat gttggaaaaa acgatattgt
     1381 ctttgctcct tcatttactt ttatagctac agtaaatcct attttatatc aaggggctgt
     1441 accagtgttc attgactcag aacctaatac atggaatatg agccctgttg cactagagag
     1501 cgcattagaa gaagctgaaa gaaacaacca attacctaag gcaatcatcg tagtgaattt
     1561 atatggtcaa atggctgatt atgaacgaat tttagctatt gcaaaccaat ttaatgttcc
     1621 agtgatagaa gatgcagcgg aatcgttagg ttctacttat aagggaattt cgtcaggctt
     1681 atttgggaca attgggatct attcatttaa cggtaataag atcatcacaa cctcaggtgg
     1741 aggagctcta gtgtctagtg ataaaaaata tgttgagcat tcaagatttt tagcaacaca
     1801 agcaagagat caagcaccgt attatcaaca tagcgaaata ggttataatt accgtctaag
     1861 caatatttct gccggtatcg gacgtggaca aatggaagta ttgaatgata gagtagctaa
     1921 acgaagagaa attttcaatc gatataagga agctttttct ggaattgata gaattgaaat
     1981 gatgccagaa gtggatggca tggtgccaaa tcgttggttg tctactttaa cgatcaatga
     2041 ttataattct attgatcaag tagttaacgt aataaataaa ctaaatgaaa aaaacattga
     2101 aacaagagct ttgtggaaac cactacattt gcaaccagta tttcaagggg taaaaatgta
     2161 ttaccatgat aataatctta gtgtgtcaga aaagttattt aatagcggct tatgcttacc
     2221 ttctggaagt aatatgacag atagtgaaca agaatttgtt attgatgagt ttaaaaaaat
     2281 attcaagtag gtgtgatatt ttatgaacat aggaattatt ggtttaggtt ccatggggaa
     2341 aagaagactt agactcattc aagaacactt taatgatatt ttactatgtg gtgttgatac
     2401 taatgaggcg agacgaaagg aagttgaaaa ggaatttgga ataccaactt ataatagtct
     2461 aacagaggca aaaaatttgt ttgaaataaa ttcagttttt gtttgtacat cccctataac
     2521 ccatgaattt attataaaag aggctttaaa tt
//
LOCUS       DACCXM010000208         2543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCXM010000208 DACCXM010000000
VERSION     DACCXM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.218"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            39..2480
                     /locus_tag="IVY61_002781"
     CDS             39..2480
                     /locus_tag="IVY61_002781"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP7971833.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          846 a          456 c          605 g          636 t
ORIGIN      
        1 cagcaaagaa tggtatattt ttgtaggagg tggcttactt gaaagaagtc gttatgatag
       61 atgctgcaag aacgccgata ggaaagtatc gcggaagttt aagtccattt actgcagttg
      121 aattaggcac acttgtgact aaaggattat tagataaaac aaaactgaaa aaagataaaa
      181 tcgatcaagt aatatttgga aatgtcctgc aagctggtaa tggtcaaaat gttgccagac
      241 agattgcttt gaacagcggt ctccctgtag atgtcccagc aatgacgatc aatgaagttt
      301 gcggatctgg tatgaaagca gtgatcttag cacgccagct gatccaatta ggtgaagcag
      361 aacttgtgat agccggcgga acagagagta tgtctcaagc acctatgctg aaacagtatc
      421 agtcagaaac aaatgaatat ggtgaaccaa tttccagtat ggtcaacgac ggattgactg
      481 acgcattttc aaatgcacat atgggattaa ccgcagagaa tgttgcaaca caattttctg
      541 tgagcagaga agagcaggat cgctatgcct tgtcgtccca gttgaaagca gcacatgctg
      601 tcgaagccgg tgtattttct gaggagatca tcccagtcaa gatttctgat gaagacgtgt
      661 tatctgagga tgaagcagtt cgtggaaata gtacattgga aaaactgggc acgttacgta
      721 cagtattctc agaagaagga actgtaacag caggaaatgc ttccccgttg aatgacggtg
      781 cctctgtggt gatccttgca tccaaagaat acgcagaaaa taataatctg ccttatttag
      841 caaccatcaa agaagtagcg gaggtcggta ttgacccaag tatcatggga atcgctccaa
      901 tcaaagccat tcaaaaattg acagatagat caggaatgaa tttatcaaca atcgatcttt
      961 ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt tagccaagaa ctgcagttag
     1021 atgaagaaaa ggtcaatata tatggaggag caatcgcatt aggccatcca attggtgcaa
     1081 gcggtgcacg tatcttgact acattagctt acggattgct tcgtgaacag aagagatacg
     1141 gtattgcttc attatgtatt ggtggaggtt taggcttggc agtcttgtta gaagcgaata
     1201 tggagcaaac acataaggat gtgcaaaaaa aaaagtttta tcagcttacg ccatctgaac
     1261 ggcgcagtca attaatagaa aaaaatgtat tgacacaaga aactgcgttg atttttcagg
     1321 aacaaacact ttctgaagag ctctccgatc acatgatcga aaaccaagtg agtgaagtgg
     1381 aaatcccaat ggggatcgcg cagaattttc aaatcaatgg caagaaaaaa tggattccga
     1441 tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa tggtgcaaaa atctgtggaa
     1501 atatctgcgc agaaacacct cagcggctga tgcgagggca aatcgttcta tcaggaaaat
     1561 cggagtatca agcagtgatc aatgcagtta atcatcgaaa agaagagctg attctttgtg
     1621 caaatgaaag ttatccttca atcgtgaaac gcgggggcgg tgttcaagat atctctactc
     1681 gggaattcat ggggagtttc catgcatact tatctattga ttttttagtg gatgtaaaag
     1741 atgcaatggg cgctaacatg atcaactcga ttcttgagag tgtggcaaac aagttacggg
     1801 agtggttccc tgaagaagag attctattca gtattttaag taattttgct acagaatcat
     1861 tggcttctgc ctgttgtgaa atcccattcg aacgcttagg gagaaacaaa gaaatcggtg
     1921 aacaaattgc caaaaaaatc caacaggcag gggaatatgc gaagcttgac ccttaccgtg
     1981 cagcaacgca caacaaaggg atcatgaatg gtatagaagc tgtagtggca gctacaggaa
     2041 atgatacacg tgcggttagc gcttcgatcc acgcctacgc ggcaagaaat ggattatacc
     2101 aaggattaac agactggcag atcaaagggg ataaactagt tggaaaacta acggtgcctt
     2161 tggctgttgc cacagtaggc ggagcttcca atatcctgcc aaaagcaaaa gcatctttag
     2221 ctatgttaga tattgattca gccaaggaac tagcacaagt aattgctgca gtagggttag
     2281 ctcaaaacct ggctgctttg cgtgcattag ttacagaagg aatccaaaaa ggccacatgg
     2341 gacttcaagc tcgttcatta gcgatctcaa ttggggctat tggtgaggaa atcgagcaag
     2401 ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca gacagctata caaatattgg
     2461 aaaaaatccg ggaaaaataa aaaagcaata tctttagaag aataaagcaa gatttacagt
     2521 tattttattt actgcagatc ttg
//
LOCUS       DACCXM010000209         2490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCXM010000209 DACCXM010000000
VERSION     DACCXM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.219"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            64..972
                     /locus_tag="IVY61_002782"
     CDS             64..972
                     /locus_tag="IVY61_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP7971834.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="IVY61_002783"
     CDS             972..1592
                     /locus_tag="IVY61_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971835.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..2045
                     /locus_tag="IVY61_002784"
     CDS             1596..2045
                     /locus_tag="IVY61_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971836.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            2045..>2490
                     /locus_tag="IVY61_002785"
     CDS             2045..>2490
                     /locus_tag="IVY61_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971837.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRR"
BASE COUNT          878 a          376 c          570 g          666 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1981 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtggggacgg
     2041 tttaatggct gatattgttc aattaaaaga agacggagtt gctaaatact taaaaacgca
     2101 tgtagaagct attgatggta aagaagcatt ggtgcagaca gatggagacc aagccattgc
     2161 aggacataag aatttttcag gttctgtaac aatcaataat aaacgtgttt tgacgactga
     2221 tgataacaga tatgaagtgg taaatctggt tgtcactaac ggtaatacag ggacagcaaa
     2281 gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagtag
     2341 tggcgggaat gattcggtta ttttaactgt tccagaaggc tatcggacac caattagttt
     2401 tgaacaactg gttgggtcaa tagaccgttc tactttgaac agtgctcaac tatctattgg
     2461 cgcagacgga gccattaaat ggagaagaaa
//
LOCUS       DACCXM010000210         2479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCXM010000210 DACCXM010000000
VERSION     DACCXM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.220"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(96..1139)
                     /locus_tag="IVY61_002786"
     CDS             complement(96..1139)
                     /locus_tag="IVY61_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP7971838.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1516..2469)
                     /locus_tag="IVY61_002787"
     CDS             complement(1516..2469)
                     /locus_tag="IVY61_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP7971839.1"
                     /translation="MKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMILMIF
                     IFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWLCAVI
                     FQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFFWISL
                     ICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLVVALF
                     ALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYSSVLD
                     FLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT          886 a          463 c          474 g          656 t
ORIGIN      
        1 attgttgttg ttagaaggat atctgcaaaa ttaaaaacag ttgtgattcg gacattgttc
       61 catgcgagaa tcacaactgt tttttcatgt tcattctaaa ctttgatcag ccaaataaat
      121 agtaaagcca tcactgtttg gagaagcccg acagctaatc cataagataa gaagcgtgta
      181 ccttctttca tgaattttgc aaagtctaaa cgcagaccaa ttgccgctaa agcagtgatc
      241 tcaaaccagc tgctgataaa atgtgccgat tggtcaataa ctacaggtaa agaaaacaag
      301 ctgttgacca aacaaaagat cacaaaccct aatacatacc aaggaagcgg taattttcgt
      361 tttttctgcg tatcagttgg cgtttcaaac agagctggtt gtttcttgtg catgaatttt
      421 tcaaacagat agacaacaac aacgagcatc ataatacgta gaatcttaaa tagcatggcg
      481 aattcgacaa cagaattgtc aagtaagctt gccccagcga ctacttgacc aacagattgg
      541 agtgtaccac ccaaaagtgc acttttttca atgagatttc caccaaacaa ctgtaatcca
      601 agaaaaggga gagtcaacat catcacagta cccagtagat tgactagtgt gatgatctgt
      661 cctttttctt cctctttcgc atggatggaa ggggcgattg aagcaatagc agaagaacca
      721 caaacagcgt ttcctccagc catcatcaaa gaagaagtat cgctgaattg cagtttttta
      781 ccaatcaagt aagcaccgat gatagtgact gacatttgca gtaacacaaa aacaattcct
      841 tttacaccaa tttgagcaat cgtttgagct gtgacagttg cacctagtaa aaaaacagag
      901 aattctaata gtttgctttc agcaaatttt gtgccgattg ctagaacagg ctgtttaaaa
      961 aaagtatttc ctaataaaat acccaataaa atagcaatag tcgctccacc taactgtggc
     1021 agataaatag caagaagcct gctgaaggca gcgatggcaa aagaaagcag taaaccgggt
     1081 aagatttcgc ggaccgctga atcctttaca gattttattg gatgtgacaa tgatttcatt
     1141 tgatcggttc ctttcgtcaa taaatactaa aagcatactc gaacatagta gcactaaacc
     1201 agtattttga caaaagaaca agaggtcgtg acaaaagtct tgtacgacct cttgtttcga
     1261 ataaacggtg ttccagaagt agcttctacg gaaataagcc gaaatttcac aaaaatttga
     1321 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctt ttacgacctc
     1381 ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag tacaaaaaca
     1441 tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct ttttcacaac
     1501 ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt tgctatgttc
     1561 tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac ttgcaaacag
     1621 acattccgca acttttaaga aatccaatac gctactatag atacctaaac tgttgaagta
     1681 atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa aggctgcata
     1741 gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa cgaaatacaa
     1801 tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag caaagttttt
     1861 aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca gcggacgtgt
     1921 cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca ataagaacgc
     1981 atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga agttcccggc
     2041 agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat aaattgcttc
     2101 aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa ggatcacttg
     2161 gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt catatgaaac
     2221 aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg aggcgatgat
     2281 tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa caaaaatgaa
     2341 tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat ggtgatagtc
     2401 tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg ggatcggaat
     2461 aattttcaaa aagttcttc
//
LOCUS       DACCXM010000211         2399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCXM010000211 DACCXM010000000
VERSION     DACCXM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.221"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            207..2069
                     /locus_tag="IVY61_002788"
     CDS             207..2069
                     /locus_tag="IVY61_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7971840.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2399
                     /locus_tag="IVY61_002789"
     CDS             2069..>2399
                     /locus_tag="IVY61_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7971841.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatt
//
LOCUS       DACCXM010000212         2310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCXM010000212 DACCXM010000000
VERSION     DACCXM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.222"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            11..727
                     /locus_tag="IVY61_002790"
     CDS             11..727
                     /locus_tag="IVY61_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7971842.1"
                     /translation="MKKEMYIMIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIF
                     PATFDEIYRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLEN
                     ADTLLTELRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPI
                     SYLLLLEKFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFY
                     QADKKIRSLWEIKNFLGVIS"
     gene            724..1842
                     /locus_tag="IVY61_002791"
     CDS             724..1842
                     /locus_tag="IVY61_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP7971843.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            1835..>2310
                     /locus_tag="IVY61_002792"
     CDS             1835..>2310
                     /locus_tag="IVY61_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7971844.1"
                     /translation="MRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLGS
                     PIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELPE
                     LWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEK"
BASE COUNT          858 a          300 c          459 g          693 t
ORIGIN      
        1 atttatttag atgaaaaagg aaatgtatat aatgattaaa gcggtgatct ttgatttaga
       61 tgacactctt gtgtctgaat atgattatgt aaaaagtgga tacaaatgtg tagctagaag
      121 attagcagtg caaaaaattt tcccggctac ttttgacgaa atatatagag tccttttaga
      181 tctttttgct gaagatagta aaaatgtatt taatcgtcta tatgactatt ttgaaattcc
      241 ttatagtaaa aaagaaatta aagaactggt aaatgtatat cgagaacaca aaccagacat
      301 aaatttttta gaaaatgcag atactttatt aacggaatta agaagtagag gatacaagct
      361 ggggattatt acagatgggt atgctataac ccaacgaaac aaattggatg ctttgaaagt
      421 agactatttg gtagattata tttgtattac tgacgaatta ggccgtgaat tgtggaaacc
      481 acaccctatc tcatatcttt tattgttaga aaagtttaat ttaaaggcac aagagtgtat
      541 atatattggc gataatgagt tgaaagattt tataactgcc aaatcaatag gaatgcaaac
      601 aattaaaata gaaagagtaa atggtattta cagagaaagc agtttgaagt tcaaaagatt
      661 ttaccaagct gataaaaaaa tacgttcgtt atgggagata aaaaattttt tgggagtgat
      721 ttcatgaaaa gtaaaagaat tctattagct tctccacata tgagtgaaga aggatatgaa
      781 aaagagtatg taaaagaagc atttgataca aattgggttg ctcctttagg tgcgaatgtg
      841 aatggatttg agaaagaatt agcagaatac gtgggagcta gtcatgctgc cgcattatct
      901 tctggaacag cagctataca tttagctttg aaagcggctg gggtcggtaa agatgacata
      961 gtgatttgtc agtccttgac gttctcagct actgctaatc caattctcta tcaaggagct
     1021 atacctgtat ttgttgatag tgattacgaa acttggaata tgaaacctga acatttgcga
     1081 acagcactag aaaaatatcc taacacaaag gctgtattag tagtacattt gtacggttta
     1141 tcagctaatt tagacgaaat tactaaaata tgcaaagaat acgatgttac tctaattgaa
     1201 gatgctgcag aatcattagg aacaacatat aaaggaaaat atactggtac atttggcgac
     1261 tatggaattt attcttttaa tggaaataag ataatcacaa cttcaggtgg cggaatgctt
     1321 gtatccaatg acaaagaaaa ggtggaaaaa gtgagatttt gggctacgca atctcgtgag
     1381 aaggcacgcc attatcaaca caatgaaatt ggctataatt atcgaatgag caatatcgtt
     1441 gctggtatcg gtcgaggaca attgaaagta ttaaatgaaa gaattgagaa aaaacgatat
     1501 attcatagtt tttataaaaa agaattgtct aaattgtcaa atatacaatt catgccagaa
     1561 aataattgga atgaaccgaa ttattggtta agtgtcataa caataaaggg aataaatgcg
     1621 aatcatctta ttgatgagtt agacaaaaat aatatagaag cccgcccagt atggaaacct
     1681 atgcatttac aacctgtttt taaaaattat gattatattg gcgatgatgt agcagaaact
     1741 ttatttgact taggagtatg tttaccatca gatacaaaga tgacagatga agatttaaaa
     1801 tatgtagtat ctacgattaa aagagtgatc aatgatgcgt aaaaaaactt tctatcagct
     1861 ttttgggaaa agaattttag atatcctatt gtcaggaatt gctctcatcg ttttatcgcc
     1921 gataatctta attgtaggtt ttttagtaag aattaaactt ggttctccaa ttatcttcaa
     1981 acaagaacga ccaggaaaaa gtgaaaaaat tttttccatg tataaatttc gtacaatgac
     2041 tgacgaaaga gaccacaatg gagaatattt acctgatgaa attcgactaa ctaaatttgg
     2101 gaaaatgctt agagcaacaa gtctggatga gctacccgaa ctttggaata ttttgaaagg
     2161 tgatatgtcg atcgtaggac ctcgtccatt attagtggag tatttgccac tatatagtga
     2221 gaagcaacgg aaaagacaca acgtaagacc aggattaact ggactggctc aggtcaatgg
     2281 aagaaatgct attagttggg aagaaaagtt
//
LOCUS       DACCXM010000213         2272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCXM010000213 DACCXM010000000
VERSION     DACCXM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.223"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..191)
                     /locus_tag="IVY61_002793"
     CDS             complement(<1..191)
                     /locus_tag="IVY61_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971845.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFT"
     gene            complement(522..755)
                     /locus_tag="IVY61_002794"
     CDS             complement(522..755)
                     /locus_tag="IVY61_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP7971846.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(770..1720)
                     /locus_tag="IVY61_002795"
     CDS             complement(770..1720)
                     /locus_tag="IVY61_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7971847.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1717..>2272)
                     /locus_tag="IVY61_002796"
     CDS             complement(1717..>2272)
                     /locus_tag="IVY61_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7971848.1"
                     /translation="GITFSSFLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITI
                     GVSVLVLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGT
                     ILIVAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTA
                     AFLFVISFLISPKQKFLRKKERSL"
BASE COUNT          731 a          426 c          284 g          831 t
ORIGIN      
        1 ttggtaaact cttcattata catattaata agcgcattaa tatgcggctg aataatttct
       61 tcaaataacg cttccttaga agtaaaatgt ttataaaaag cacccgtagt tacgcctgcg
      121 gtcaggcaca gttgccttat cttagtttgc tgaaatcctt ctttttcaaa atggcttagt
      181 ccactttcta ttaacttttg gtgagttata atataatctc tggtttctat gggtacttcc
      241 tcctaataag atgaattaac tttattatct tacacaggac ttgaagtttc aatcgttaac
      301 gtttattact gaactcattc cttagagcta aactaatgag aaagagcaga ataactgata
      361 caaaaagcaa tcctacaaga attaatagac tcatctaaat cattcacctt ttctaaaata
      421 atccgagaaa gatttaaaaa aattatatcc agctattagg ataagtccta tcataatcac
      481 tccaatagcc attttcttat cctccctaaa aataaagtct tttacttaaa accttttttg
      541 taatcgtcaa taaatttttt ccctttatca gttaattgaa atcctatatc taaatagtcc
      601 tcatatctaa gtttagccag catctcactt acagatgctg gactaacact tagaactttt
      661 gctaaatcat tattttttaa tttctttttt ctttttgata attcctgaat tgctagtaaa
      721 taaattagta tagtagtcgt tttaatttgt accataccta ccctccaaat tatttcgaaa
      781 ggccttcagc aattttatcc aggttccatt tcatcatcgc atagtaacta tcaccatctt
      841 gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata gataggaata ttggtatctt
      901 ttgatactgt tttcattggt ctatcatcca cactgctttc tacgaataag gagggaactt
      961 ttgttgtgcg taatttttca actaagtgtt ttatttgatc tggtgttcct tcttcttcag
     1021 tgttgatttc ccaaatgtaa gcagaaggca cattatacgc tttcgagaaa tatttaaagc
     1081 atccttcact tgtaacaatc atttttttat cattcggaat tgaagcaaat ttagatttag
     1141 cttctttgtc tagagaatcc aatttttcaa tatacttatc tagattttct ttatagaatt
     1201 ttttattatc aggatctttt tccgctaact gtttttcaat atttttagcg taaataatac
     1261 cgttttctaa atttaaccaa gcatgaggat cttccttccc tttctcactc tgaccctcta
     1321 agtaaataac atctatgcca tcacttgctg caaaatagtc tttgttttcc tctttgttcg
     1381 catttttaac taattttgta aaccaagcat ttcctccagt ttccaagtta acaccgttat
     1441 aaaaaatcaa atctgctttt gaagtttttt gaacatcttc aggcaaaggt tcatattcgt
     1501 ggggatcttt cccaataggt acgatactgt gtaaatcaat tttatctttt gctatatttt
     1561 cggtaatatc tgctaaaatc gaattagtaa ctactacttt taatttttcg ttcgaagcca
     1621 ctttatcttt gtctttacta tttattgaac atgccccgaa tataaaaaga gcaatcaaaa
     1681 tccccagact tcccagtact gctttccatt ttctcatcat aacgatctct ccttctttct
     1741 taaaaatttt tgttttggtg agattagaaa actaattaca aataagaatg ccgctgttaa
     1801 aacaatactt gatcctgctg ctatgttaaa actataacca ataaacaatc caagcaatga
     1861 tgaacttgct ccgactgttg cagaaataaa tatcatagtt ttcaagctat ttgcatataa
     1921 atatgctgta gctgctggtg taattaacat tgcaacaatt aatatagttc ccacactttg
     1981 catagctgtt actgctacta aagttaatag aaccatcaat agataatgat aaaaactgac
     2041 attcatccct attgcttttg ccattaatgg atcaaaagaa gtgattaata attctttaaa
     2101 aaatagaact actataacca acactaaaac cgaaacgcca attgtaatcc acttatcaat
     2161 atcttgtaca gctaaaatat ttccaaataa aatatgaaat aaatccgttg agctatttgc
     2221 aactcctata aggataactc ctagtgctaa aaaagaactg aaagtaatac ca
//
LOCUS       DACCXM010000214         2262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCXM010000214 DACCXM010000000
VERSION     DACCXM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.224"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(151..2148)
                     /locus_tag="IVY61_002797"
     CDS             complement(151..2148)
                     /locus_tag="IVY61_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP7971849.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          576 a          505 c          411 g          770 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aa
//
LOCUS       DACCXM010000215         2207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCXM010000215 DACCXM010000000
VERSION     DACCXM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.225"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..525)
                     /locus_tag="IVY61_002798"
     CDS             complement(<1..525)
                     /locus_tag="IVY61_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971850.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTT"
     gene            complement(587..1267)
                     /locus_tag="IVY61_002799"
                     /pseudo
     CDS             complement(587..1267)
                     /locus_tag="IVY61_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1589..1810)
                     /locus_tag="IVY61_002800"
     CDS             complement(1589..1810)
                     /locus_tag="IVY61_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971851.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(1908..2153)
                     /locus_tag="IVY61_002801"
     CDS             complement(1908..2153)
                     /locus_tag="IVY61_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971852.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          636 a          439 c          326 g          806 t
ORIGIN      
        1 cgtagttgga tacgtagatg aaacctcaac tatagttctt ttccccgtac ctttaataga
       61 cactaaacca ttgtcatttt ctatagtgct ctttttccca tttactttca caactgttcc
      121 ccaataattg aatacaggta atgtgatatt ttgctcatcg gcatcttcta ctgtatagac
      181 tattttggaa ttatatgctt tatgagatgc attctcccac cgatcaccaa cttttacttc
      241 atgtttcttt aaatgatctt tatatttcaa tgcatctaat ggtgcatagt catataggcc
      301 tccagttctt gaggatgtat aacgtgtaac atcttttttg gtcataacta aacgaccttt
      361 atacgactga attttatgcg aataatttgc ttctgttgct gcagtcacag taaaaataat
      421 aagaactatt cctacaaggc tccacatact ttttttgttg cttgttttcc actttaaaaa
      481 cactatggtt aagattaatg aaccaaaaag cacttgaaaa cccaaggttc tcggttctgt
      541 tgcgaagttt gaaaatcaaa cgcgccctct ctgaactcca aatatcttag gctgttattc
      601 ccattaatac cttgatttca gtagacaccg aaaagccgaa gagcgttcca tttcttcggt
      661 tctttttata tattcctcga atggtctcca tgcccttaat cgtggaagag gctgtacgga
      721 gactttgata aaatttattt cgtcgtttaa taggtcgatg gtcttgttct attaaattgt
      781 taagatactt cacagttcgg tgctctgtct tagtatataa acccacactc tgtaactttc
      841 taaaggcgga gccaagagaa ggtgctttat cggtcacaat tgctttcggc tcaccaaact
      901 gtttatggag tcgttttaag aaagcatagg ctgcttgcgt atcccgtttc tttcgtaacc
      961 agatatctaa ggttaagccg tccgcatcaa ttgcacgata aagataatgc caacgtccct
     1021 taattttgat ataggtttcg tccattttcc atgaatagaa ggattgtcta tttttcttct
     1081 tccaaagata atagaggact ttgctgtact cttataccca acgataaatc gtagtatgac
     1141 aaacatttat tccacgatca tataacaatt cctgaacttc acgatagctt agattgtaac
     1201 gcaggtagta accaacagcg acaataatga cgtctttttt gaattgtttg cctttaaaat
     1261 gattcattac tctgtcctct ctgtcttttt tctcaatttt acactaaaat agattttttg
     1321 gaaaactttg caacagaacc gtttttacta tatacttctt cccagaactt gtccgtctcc
     1381 ttcaaattat ctctacctct cgagttcatt gggtggtttg cccatttatt aaacatggaa
     1441 tcacctttct catactcata tatttatgtc ctatttcaat tatatttata tatgcttaaa
     1501 taaatttgta tcctgaaatt tattgtaata tgaagctgta ttttacatat agtaaccaaa
     1561 gagttaataa aataaaaaga gcgaaccatt atagatcagc tcttttttca ttgctgtttt
     1621 taatcttatc tagaatcact tcaaatgttt caaaggtatt tacatctaac tgacataacc
     1681 aaagcaagta cttcttttct ttgtaagtcc aatcaatatt acggtaagag gacattagat
     1741 tatggtacct tgctctttgt tcaactgaag ccaattccca gcgtttttca aatatttttt
     1801 cttcttccat tattgatccc tcgcaagacg ctacctcaaa cttaaataat gggcagcgaa
     1861 agtctaattc cttagatcta agtatagcaa acaaatatga aaaaaactta gaaggtagta
     1921 aaagttcttc cgtcttttgc tttgcttcta ttcttaatgt gtttcgttat cggtgcccta
     1981 caaagatttt cattaattac acactcattt attgttgtgt attttctgac atttctctct
     2041 tgatcaatta ttttaattcc atgggcatcc tgatagttca taggagagga gcggctatca
     2101 gaaatagttg cacctatctc ttgaacttct tttacaactt ctggaaaatc catccatcct
     2161 gcttcagctg tcaaaagtaa taatctataa tatttatctt tcctttt
//
LOCUS       DACCXM010000216         2178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCXM010000216 DACCXM010000000
VERSION     DACCXM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.226"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(658..858)
                     /locus_tag="IVY61_002802"
     CDS             complement(658..858)
                     /locus_tag="IVY61_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7971853.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(992..1600)
                     /locus_tag="IVY61_002803"
     CDS             complement(992..1600)
                     /locus_tag="IVY61_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP7971854.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(1685..2155)
                     /locus_tag="IVY61_002804"
     CDS             complement(1685..2155)
                     /locus_tag="IVY61_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP7971855.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT          701 a          438 c          315 g          724 t
ORIGIN      
        1 ttttgtgtaa cgattattat tttttaaaat ttctcaaaat tcttaactat cataccatac
       61 cgtgaaaaag atcgaaagac cgaatacacg cagttcgcaa tcgttttatc tttttttatg
      121 tattttttcc agaaaaagcg aacaacaagg tattttccaa ataatcgtgc agtaaaatga
      181 tcgcacatac ctttcccatc tttttaacag tctttgccta ccgttcagat tatcattcga
      241 tttcacgaca atttcttcct atgactggtc cttttgttaa acccatcttc tattcgtttt
      301 atttccagct cactgcatgg ctcttaaaat gactcgaact agtgattgca ttaccatcat
      361 cttttaatgg atagatatgc tcctcttcaa agtaaaacga ttttctttta aagtaataga
      421 attgacatgg taatgagtat gtatactagt ggtttcacaa atggtctttt attcaacatc
      481 caaaaactga aatttcttta aaatccatta ctaacgaaga aaatgttcct tgcttgataa
      541 ttagttagga catgtcaagt gaacaaatgc atttacttag ccaaaaaatc taaaaacttc
      601 ataaatagac aaaaagacac gactctctaa gaagtcgtgt ctttattcaa caaattatta
      661 taatttagtc acttcagcag cttgaggtcc gcgtgcacct tcaacaatcg tgaattctac
      721 ttcttgacct tcttctaggc tcttgaagcc gtctccttga attgctgaga aatgaacaaa
      781 tacgtcatct ccgccgtcta ctgtaataaa cccaaatcct ttttcattgt tgaaccattt
      841 aaccgttccg tgttccataa atgtatatcc tccaaaaaag ttcttgcaag taaccttgca
      901 agaacattat acccaattta gaaaacgcta gcaagctttc gctcttttat tttccaaatt
      961 atttttaaga ctgatatgac ggaggaaatg gttattttgc tgcttcgaaa cgtttgttta
     1021 cttcatccca attaataaca ttccagaatg ctgcaatata atctggtcgg acatttttat
     1081 atttcaaata ataagcatgt tcccaaacgt ccaaacctag aattggtgtc tttccttcca
     1141 tcaatggaga atcttgattt gcagtagagg taatagctaa ttttccattt tccattacaa
     1201 gccaagccca tccagaacca aatcgtccag cggctgcttt tttgaattct tctttaaaag
     1261 aagaaaaatc accaaaagct tcattaatcg cttcttttat ttctcctgta ggttcgccac
     1321 cagcatttgg tgccatgatt tcccagaaaa atgaatggtt agcatgtccg ccaccattgt
     1381 tacgtacagc tgtcttgata tctgttggaa tagcgtccat atcagataat aattcttcta
     1441 ttgttttttc gcctaattct gggtatttct caattgctga atttaaattc gtcacataag
     1501 tattgtgatg cttatcatga tgcagatgca tcgtttcttc gtcaatataa ggttccaatg
     1561 cgtcatatgc ataaggtaga tctggtaatg tgtaagtcat aaaaaaattc ctcctcaatg
     1621 ttttcttaat aatgtctaca ttacaaagag taacaaagag aaggaaattc ttcaattgtt
     1681 atgcctagtc gtctttttca ctttgaccta cttcttcttc aaaaacctca gtttctttaa
     1741 aaataaacaa cttgctgaaa atatagttcg caaggataac aataatttga gtgatgattt
     1801 tcgccacata ctcattgctg ttcataacat tgatcaacaa taccattgcc cccatgtcca
     1861 atccgtaaga aaggatacgg tagaagaaga acttaccgca ttcaataagt gcatgtgtaa
     1921 aattcggtga ttttgaatgg aatacccaaa gtttgttcgt tacaaaagca aacaatacag
     1981 aacaaaacca cgaaatagaa ttggataagg ccatacttaa atcaaaatat tggcgtgcga
     2041 gagcaaaact aacaaaattt acaatcgtag ccaaaccacc aaaaaagaga tagactagta
     2101 tctcccaata tccttttgct tctaaatatc cttttaactg tcgatactgc ttcatgagcc
     2161 aacctacctt attaaaaa
//
LOCUS       DACCXM010000217         2160 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCXM010000217 DACCXM010000000
VERSION     DACCXM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.227"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            278..787
                     /locus_tag="IVY61_002805"
     CDS             278..787
                     /locus_tag="IVY61_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7971856.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IVY61_002806"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IVY61_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7971857.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
BASE COUNT          754 a          374 c          417 g          615 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
//
LOCUS       DACCXM010000218         2098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCXM010000218 DACCXM010000000
VERSION     DACCXM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.228"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            78..935
                     /locus_tag="IVY61_002807"
     CDS             78..935
                     /locus_tag="IVY61_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7971858.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            936..1484
                     /locus_tag="IVY61_002808"
     CDS             936..1484
                     /locus_tag="IVY61_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971859.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1744..>2098
                     /locus_tag="IVY61_002809"
     CDS             1744..>2098
                     /locus_tag="IVY61_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971860.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
BASE COUNT          807 a          267 c          446 g          578 t
ORIGIN      
        1 tttttttggt agtaactaaa aataaatatg ttataatagt atcaaatgaa tattgctaaa
       61 agaaaagagg taggaacgtg gtagcgaaga aatatgtaat cggtaatttt aaaggtggcg
      121 taggtaaaag tacgtgcgcg caaatgttag ggtttgaatc agcagttaat gaaggaaaaa
      181 gaaccttgat aattgacttg gatatgcaag gaaatacttc tgatgtgatg aacttaacgc
      241 atatgaactt cactaacgaa gaaggaggcg gagaaggtga accgattgtt tacgaaaata
      301 ctgtgactga cgtgttgata aacaatcttc cacctaaaga ggctgtctat aaagtaatca
      361 ataacctcta tatacttcca gcagacatgt cctttgaaat gtatgatgat tggatcaaag
      421 accaatataa tgattcatta tcccaattca aatatatgaa agaaaagcta gaaccattat
      481 ttgatgagtt tgacgtcatc tatttagatg tcccaccttc tatttctgta tatagtaaat
      541 ccgcgatgta cattgcggat tgggcaatcg tcgtcttaca gacccaagta aaatcgatga
      601 gaaatgcgat gcaatatctt gagtatatgg acttttttgt gaaagaattt gatacaaatt
      661 tatatgttgc tggtgttatt cctttcatgt tggaaagcgg tgacgcagta gatcaggaaa
      721 tgtataaaca agcccaagag atttatgggg aacatttgat taaaaatgtt gtactcaaaa
      781 atgcgcgctt aaaacgatac gacggatcag gtattacgat ggagaagaca aagaaaggtc
      841 tcttaaaaca atgggatcgc aaaagccacg aattatttat cagtattttg aaagaattgg
      901 aagaacacga gaattggtat gcggaggtgg agtaattggc tgacatcaaa ggacttgata
      961 tttttgggaa caacaaagga ctcgccaaaa taaaaaaaga agcagaagca aagaaaaagc
     1021 agcaagcaat tacaccatct gaaaataagc aattagaagt gcaccaagtt caagaaataa
     1081 aaacttcctc caatgttgaa agacagagta gaggaagaac aaaaaaagtt ggcgcaccaa
     1141 tcagaaagtt tgataaggtt tattccacca agcatccttt aaaactgtcg actttgttaa
     1201 atgcgacttc cagagtttta gtagaaaagt atgagactaa tatgacaaga gatgaattgc
     1261 ttagaaaagc tttagatgag tatattaaac agaatcttac gcaagaagat aagcaagatt
     1321 tgtttaatga cgtggtgaaa gaactagact tgttcagaga aaaatatcca attgttccag
     1381 aattagatga tcgtggagaa ataataagaa ctaccagtca gataaaacaa gataccgaag
     1441 actcactgcg caaagggtgg ggaatgaata ataaagttag ataataatca aagaagaact
     1501 aagtaactac ctttaaacgg tagttactta gttcttcttt gatttgcgtg aaatagaggg
     1561 gatttatttt tctggttacg tgattaaaaa atccaaaaaa tgagcatagg aacggttatg
     1621 attttagggt gtgttttaat gagatattgt gatttcatta acaagtgtat gataatctgt
     1681 tgttgagtta gtaaataagt aatagagcta actctaaaat aaaactatta ggaggagatg
     1741 tagatgaaaa agtttaaaaa gaaaatatat ttaggattgg ttgttggggt gagtatgttt
     1801 gggttcatgg ctcatactaa acaagtaact gcagatacta accaaaaaga aaggattatg
     1861 agccaattag atgactctca aatagagtat gtacccaatg agctaattgt cagtttaaag
     1921 gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg tgtaaaagtt
     1981 atggtggaaa aagatttgac tcttaggata aatgaaagcg attataataa gcgagatcaa
     2041 gtattattag tgaaattgaa cgaagaaatt agtgtggaag aggcagtaag aacattta
//
LOCUS       DACCXM010000219         2092 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCXM010000219 DACCXM010000000
VERSION     DACCXM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.229"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>2092
                     /gene="acm"
                     /locus_tag="IVY61_002810"
     CDS             <1..>2092
                     /gene="acm"
                     /locus_tag="IVY61_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP7971861.1"
                     /translation="LFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIK
                     VKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQV
                     VFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSS
                     SVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIH
                     LGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIEN
                     PEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTE
                     IGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFD
                     PLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTN
                     NWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIE
                     NGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWR
                     YRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWK
                     NFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSS
                     KKSSFAVIIGLLIVT"
BASE COUNT          819 a          343 c          407 g          523 t
ORIGIN      
        1 gttatttatt attactaata taacatcact gattcctgtt catgtctatg cggatgcagg
       61 cagagatatc agcagtaatg tcacttcgtt gaccgtggac ccaacgaata ttacagatgg
      121 aggaaacata aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga
      181 ttatctttgg atcaattggc caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga
      241 aatcccatta atgattgaaa ataaaaatgt tggaacttta acggtcagaa aagattctgc
      301 gcaggttgtg tttaatgaaa atattaaaaa cctagactca gtagaaggtt gggggcaatt
      361 tgagatacag gccagaaacg taaccgatac cggtgaagaa aatacgggac cattcaccgt
      421 gacgagcggt gataaaacag ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc
      481 tagcgttttt tattataaaa caggagacat gctacctgaa gatactaagc acattcgatg
      541 gtttttgaat attaacaata acggcactta tgttgaacaa ccagttaaaa tcagtgatga
      601 aatccaaagt ggacaaagat tagatccaag tacgtttgaa attaatcaaa tacacttagg
      661 tgaacagaag gtttatagag gagaagaggg aatccaacaa tttttacaag atttccccag
      721 cgctacattt aatttttcag taactgataa ttacattgaa ataactatac caaaaaactt
      781 tgtcaatctt aggaaaataa tggtgagtta taagacgatc atagaaaacc cagaacaaat
      841 aaattttgag aatcattcgg aagcttggtt taaagaattt aataaacctg ctgttgatgg
      901 agaaagtttc aatcatacag ttaaaaatat ttcagcttct ggtggagtca acggtacagt
      961 tagaggcgaa ttgaagattt tcaaatatat caatgacaca gagattggta tcccaaatgt
     1021 gactttcgaa ttaagacgag cagatgaaca gcctattcaa ggacagtcta gtatcctgtt
     1081 gacttcgaat gaacagggag aagctagtat caaaggcctg caagtaggag actatgttgt
     1141 aaaagaaaaa gaagcaccca attggattga ttttgatcca ctaagttcaa atgaactcaa
     1201 attctccata aacgaaaatg atacagaggg agtcagttta cctatttata acaagaaaaa
     1261 ggttacgaac atcacagcaa ctaaaatttg gaacggtgga accactccaa gaccgagtat
     1321 ttattttaaa ttattcagag caagtacaaa caattgggaa ccagttcctg atgcagaaac
     1381 aaaacgatta gacaatggaa taacttcagt gacttgggaa gatatccaac agtatgatga
     1441 tagtggaaac gaatatacgt ttaaagtgca agaagtagat caaaacggaa atgattatgt
     1501 cccttcaggt tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt
     1561 catttcagtc tcaggacaaa aaacatggga agacaatgag aaccaagacg gcaaacgacc
     1621 gacgacgatc acagtaaatc tattagccga cggtcaaccg atccaacaca aagaagtaag
     1681 tgaaaaagat gattggcgct atcgtttcac aaatctgcca aaatacagag acgggcaaga
     1741 aatcatctat actgtaacgg aagacaacgt gcccgaatac agtacgacga ttgacgggta
     1801 taacatcaaa aacagttata caccggaaaa aacaaatata tctataccag gtagccatat
     1861 aacaccgtgg aaaaattttc cagagaagac agagagagag aaaattagaa atctattctc
     1921 tcaaaaattg caaatatttg ctcatacagg agaatccagc gaacaaagaa gcaattatat
     1981 tagtaagaga gctgtaccta aacagataac aaataaatca aaaagcattc ttcctaaaac
     2041 aagtagtaaa aaatctagtt ttgctgtaat cattggttta ttgatagtaa ct
//
LOCUS       DACCXM010000220         2080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCXM010000220 DACCXM010000000
VERSION     DACCXM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.230"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..369
                     /locus_tag="IVY61_002811"
     CDS             <1..369
                     /locus_tag="IVY61_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7971862.1"
                     /translation="KPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIM
                     EIFNQKNVGGTCEDYKNALVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISI
                     MFNFKMYKTAYNMLWRIYNR"
     gene            383..1462
                     /locus_tag="IVY61_002812"
     CDS             383..1462
                     /locus_tag="IVY61_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7971863.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKINDKKVK
                     TIIIPNPIKENLPYYNKTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIY
                     GEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEAL
                     AIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQK
                     GIKLRKELTSKNISNIWYEYLNQFF"
     gene            1505..>2080
                     /locus_tag="IVY61_002813"
     CDS             1505..>2080
                     /locus_tag="IVY61_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HAP7971864.1"
                     /translation="MFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAICTWHWRKGD
                     SIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSESDMIMVKWY
                     SCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIVVPIVFVSK
                     LLEVRIALVHGYNALYYGDYATQSGYLQIILY"
BASE COUNT          784 a          229 c          400 g          667 t
ORIGIN      
        1 aagccaaaaa gtaatttggt ttttagacat ggaaatatga ttgctgatta tgaaaagttg
       61 ataaggcaaa atggaaacaa cataatagat aaaaaagctt gtatgttggt tgcatttgat
      121 gtactaatta tggagatttt taatcaaaaa aacgttggtg gaacatgtga agattataag
      181 aatgcattag tttctttgaa acatcataaa atattgagag aaaatatttc aaatatagtt
      241 agaatgaaag catatgaact tgaacctaat atgagaaaga agttagccct tgtgaccatt
      301 agtataatgt ttaattttaa aatgtataaa acagcgtata atatgctctg gagaatatat
      361 aatcgataaa gaggtaggaa atatgaggaa attgaccttt gtgataggaa ttatgggaaa
      421 aggaggggca gaacgagtaa ttgcgaattt ggcaaataat tttattcaag ataactggga
      481 aattgatatt ataactatat atgggaatcg gcaagactat gaactgaata gaaaaataca
      541 ggtacatcca ataatatgta aaagttcgtt tcgtgcattt agacctttgg aacgctttaa
      601 ggaacttagg aaaaaaataa gggaaataca gcctgatctg gttatttcgt tccttgctga
      661 tgtaaatatc catgtcatta tttcattgtt aggagtaaaa caaaaattaa ttgtttctga
      721 acgaaatgat ccacaacgga atccagaaga accatggatt aggaaaatta gagattttct
      781 ttataaaaat gtagatggaa ttgtttttca aacaccagac gcaaaaaaat attttagtaa
      841 gatcaatgat aaaaaagtaa aaacaattat aattcccaac ccaataaagg aaaatttacc
      901 ttattataat aaaactacaa gtagtaaaaa atttattact gcatgtagat tggataaaca
      961 aaaaaatttg cccttaatga ttgatgcaat aggaaaactt attcatgaag gatatgagtg
     1021 tgaactggat atttatggag aagggggatt aaaaaccgag cttgaaagtt atatcaacca
     1081 taatggatta acagaatttg ttcatctaaa agaatttaca aataaaatac atgaaaagat
     1141 gcaaaatgcg gcaggatttg taatttcttc tgattatgaa ggtatatcta attctatgtt
     1201 agaagcactg gctattggag tgccagttat cgctacagat tgtccagtag gtggcgcaag
     1261 aatgtattta ggtgatagaa atgaaatagg tcatcttgtt gaatgtaata atctagataa
     1321 gttaacagat gcaatgcgta gggtactttt ggatagagaa agtgtggtaa gaaaagcgca
     1381 aaaaggaatt aaactaagaa aagaactgac atctaaaaat atttcaaata tatggtacga
     1441 atatttaaac cagttctttt aaccaaaagg aggatattgt gtgaaagaaa aaataaatat
     1501 attgatgttt tcgttaacgt tagtcgcgtt tacaatattt tgcgcttttt cattaaatca
     1561 gaactattgg ctaaattggc ttggaatatt gatttactta tatgcaatct gtacatggca
     1621 ttggagaaaa ggagattcaa tattttcatt atacacgatt tttttcactc tttttttatt
     1681 atttagttat gggcaatgca ttatgtgggc attcggtatt ggaacagatc gtggaatcgg
     1741 tacaacagta gtagcttatg gtacggggat tattcctagt gaatcagata tgataatggt
     1801 aaaatggtat tcttgtattt ctatgtttgt atttcacata ggagcactct tatttacgag
     1861 aaaaaccaca ctaagaaaag ttagttggta tgaatctgga gatgctgatg ccgatagaaa
     1921 gtttttattt aaaatagggt gtatcatttc tataatagta gtaccgatag tttttgtttc
     1981 taaattacta gaggtacgta tagcattagt ccatggttat aatgcattat attatggtga
     2041 ttatgctaca caaagtggat atttacaaat tattctatat
//
LOCUS       DACCXM010000221         2070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCXM010000221 DACCXM010000000
VERSION     DACCXM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.231"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..253
                     /locus_tag="IVY61_002814"
     CDS             <1..253
                     /locus_tag="IVY61_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7971865.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            308..1873
                     /gene="guaA"
                     /locus_tag="IVY61_002815"
     CDS             308..1873
                     /gene="guaA"
                     /locus_tag="IVY61_002815"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7971866.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT          636 a          433 c          430 g          571 t
ORIGIN      
        1 tacggaaggc tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa
       61 aaagaaattc acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgtc
      121 ccactcaaac ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc
      181 gtttggttat cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat
      241 ctttgaaaac taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
      301 ttcaaatttg tcttcaccaa cacgatttga cgaagagcca aaaattatcg tacttgatta
      361 cggtagtcag tataaccaac tgatcactcg ccgtattcgt gagttcggtg ttttctcaga
      421 gttgatgagc caccgcatta ccgcagaaga agtaaaggca atgaacccta aaggaatcat
      481 cctttctggc ggaccaaaca gtgtttatga tgagaaggcc tttggtattg atgaaaagat
      541 ttttgatttg gggatcccag ttttaggaat ctgttacggt atgcagttga ttacttataa
      601 attaggcgga aaagttaaac cagccaaaaa ccgtgaatac ggaaaagcag aattggaaat
      661 cacttctgca gaagcggccc ttttccaaaa cactcctgaa aaacaaaccg tttggatgag
      721 tcatggtgac ctagttaccc aagcaccgac tgatttcgat gtcgttgcta ccagcaaaga
      781 ttgccccatc gcatcgatcc aaaatgcaga acgcaaaatg tacggaatcc aattccatgc
      841 agaagttcgc cattccgaat atggaaatga cttgttgcgc cattttacac tagatgtctg
      901 ccaatgtact ggtgactgga caatggataa cttcatcgac atggagatcg caaaaattcg
      961 tgaacaagtc ggtgacaaaa aagttttatt aggcctttca ggcggagtag attcctctgt
     1021 tgttggtgtt cttttacaaa aagcgatcgg cgatcaatta acatgtatct tcgttgatca
     1081 cgtattgttg cgtaaaggtg aagcagaaca agttatggaa agtcttggcg gaaaatttgg
     1141 cttgaacatt ataaaagtcg atgccaaaga acgtttctta tcaaaacttg ctggcgtgtc
     1201 tgatccagaa gaaaaacgta aaatcattgg aaatgaattt gtatatgtct tcgatgatga
     1261 agcaaccaaa cttgccggtg aagaaggcgt ttctttcctt gcacaaggaa ccctttatac
     1321 cgatgtgatc gaatcaggaa ctgaaactgc ccaaaccatc aaatcacatc acaatgtagg
     1381 tggattacct gaagacatgc aattcaagct gatcgagcca ttgaacacat tgttcaaaga
     1441 cgaagtacgt gaattaggta cccaattagg catgcctgac agcatcgttt ggcgccaacc
     1501 attccctgga ccaggcttag gtattcgtgt ccttggtgaa ttgacagaag aaaaattaga
     1561 aatcgtccgt gaatcagacg ctatcttgcg cgaagaaatc gccaatgccg gtttagatcg
     1621 cgacatctgg caatacttca ccgtcctacc aggcattcgc tctgttgggg ttatgggaga
     1681 tggccgtacg tatgactaca ctgtaggtat ccgtgctgtc acttctattg acggcatgac
     1741 cgctgacttt gcccgtatcc cttgggaagt cttgcaaaaa atcagtgtcc gtatcgtaaa
     1801 tgaagtcgca cacgttaacc gtatcgtgta tgatattacc agcaaaccac ctgcaacagt
     1861 ggagtgggaa taaagagcca acataatatg gatgaggaat acctataaat caacattatt
     1921 caattactaa agttcctgat tttcgtaaaa aataagatgg tttcctacca ttttccctac
     1981 caaagccctt gttagattaa ctactaacaa gggctttgct tttattcact acctttattt
     2041 tcctattgaa ttagcttttt atctaatcag
//
LOCUS       DACCXM010000222         2032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCXM010000222 DACCXM010000000
VERSION     DACCXM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.232"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            645..977
                     /locus_tag="IVY61_002816"
     CDS             645..977
                     /locus_tag="IVY61_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7971867.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1596..1937
                     /locus_tag="IVY61_002817"
     CDS             1596..1937
                     /locus_tag="IVY61_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP7971868.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          706 a          271 c          428 g          627 t
ORIGIN      
        1 taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt ttttgagtta
       61 taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc ttgatattac
      121 ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt taattgtctg
      181 ctatgcttgt gatgaattga ttttcgtttt gcacttttgg atgataaatt tggacaattg
      241 aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt cgtcagcgtt
      301 catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta cggtattacc
      361 cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg gctgtgttaa
      421 ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cataatgtta taaaaatgtc
      481 cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg tttggatgcg
      541 ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa aaataacaag
      601 aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca tatacaggaa
      661 ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca gagaaaatca
      721 cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc
      781 agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag caaataattt
      841 taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt tatgaccaaa
      901 acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg
      961 ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt tttcgcttgc
     1021 ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat aaggagtaga
     1081 cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg attggtaacg
     1141 taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat gaatttgtgt
     1201 aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt agaatagcgt
     1261 ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact catggaataa
     1321 ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac
     1381 atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc gctgtctact
     1441 agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt tttaagtaaa
     1501 cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat ataaaacata
     1561 gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat ccgaatatac
     1621 gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa ggatttgtga
     1681 cgtttagaag gggcggaaat tgtaaaggat tagatgtttt agcattagac gaggatgatt
     1741 tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct gaagatgatg
     1801 aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa ttatctgttg
     1861 aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt gaatttgtag
     1921 cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct tagaaggagt
     1981 cacgacgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat tt
//
LOCUS       DACCXM010000223         2031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCXM010000223 DACCXM010000000
VERSION     DACCXM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.233"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(315..398)
                     /locus_tag="IVY61_002818"
     tRNA            complement(315..398)
                     /locus_tag="IVY61_002818"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="IVY61_002819"
     CDS             926..1426
                     /locus_tag="IVY61_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7971869.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..1960)
                     /locus_tag="IVY61_002820"
     CDS             complement(1616..1960)
                     /locus_tag="IVY61_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP7971870.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          621 a          367 c          318 g          725 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga
     1861 ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt
     1921 ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac
     1981 gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta g
//
LOCUS       DACCXM010000224         2030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCXM010000224 DACCXM010000000
VERSION     DACCXM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.234"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..293)
                     /locus_tag="IVY61_002821"
     CDS             complement(<1..293)
                     /locus_tag="IVY61_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7971871.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRW"
     gene            complement(265..1464)
                     /locus_tag="IVY61_002822"
     CDS             complement(265..1464)
                     /locus_tag="IVY61_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7971872.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1594..>2030)
                     /locus_tag="IVY61_002823"
     CDS             complement(1594..>2030)
                     /locus_tag="IVY61_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7971873.1"
                     /translation="VSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPA
                     FVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDI
                     NVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          642 a          404 c          341 g          643 t
ORIGIN      
        1 aaccagcggt aagcgacatt ggtatcgact tccttaatgg tttgtcgcat ggaacggata
       61 ccgaataaat actgaataaa caccaatttg atcagaatca ctggatcgat acttgggcga
      121 cctaaatcgg agtaagtggc ttcgacgatt ggataaataa attcaaaatc aagcgcggca
      181 tcaatctttc gaacgagatg atcttgagga actagatcat caatcgtaag catcgcaaat
      241 tgatttctac cattcgtatt gtttttagac atcatagcta tcactccttg cattcgtttt
      301 cataaatcta ttataaaaga aaagactgta gacatcatcc ataaaatgga ctttgtctac
      361 agtctgcgca ttctcattag agaacactcc cttttttgtg acttttctga agctagagtg
      421 aatactttca actgcattag tagtgtacat gatttttcta atggcgctgc cataatcaaa
      481 gagttgttca acatgagaaa agtttcgttt gcatacatct actgcaccag aataatgaga
      541 ccaccgattt tgaaaactgt cgaaggcagc gtgtgcagcg tttaaagaag aagcactgta
      601 gaattttttc atgtctcgac agacttcctt ataatcctta cttgggaggt atcgtaacgc
      661 atttcgaaca agatgaacaa tacagcgctg cacaatgaca gaaggaaaaa ttgcttttgc
      721 gccttctttg agaccggaaa caccgtccat cgaaatgaaa aaaacatctt ctacaccacg
      781 tactttcagt tcatcaaata cttgcatcca gcgattttta gattctgttt gattgagcca
      841 taggcctaaa atctccttgt ttcctttaag atcatagcca agaatggtgt acacagcata
      901 ttctttagct tcatagtttt cgcgtaaagt gacgtacata caatcaacga atagaaatgc
      961 ataacacttg gctaatggcc gagcttgcca ttcttccaat tcaggaagga cggcatccgt
     1021 gatatcagaa atcatttcgt gggaaatatc aaagccgtaa atatcttcaa cggttgcggc
     1081 gatatcgcat tgactcattc ctcgtgcata catggaaaga accttacctt cgatgtcgga
     1141 gacatctcgt tttctattag gaatcaactc aggttcaaaa gaagcctccc ggtctctggg
     1201 aacatcaatg tttactttac caaagctcgt tttaagcgtc ttattgccat aaccgtttcg
     1261 tcgattgtca tgtttctttg gctctttaga atgggcgtca taccctaaat ggttattcaa
     1321 ttctccttga agcatttttt caaataaggg accaaacaca tcttttaaaa catcttgcat
     1381 atcctctaca gattcgggtt gataagtatt caaaatggat tcagctaact tctcagcatc
     1441 aggatttctt ttctttctag ccatcgtgta atcacctctt gatcttattt tagaaaaata
     1501 aaaaccgtga agcaaccacc cgcctaggat tgatggttac ttacacggtt tatattacac
     1561 tctcattttt ggtgctaaaa agcaccgaaa attttaatta ttgtcaatta ataattcttt
     1621 tggattcctt cttaatatat tgaatgaaga aataatcaat gatacaagaa gtactaaact
     1681 cataaatata actacataaa tcatgaattt aggtaataca ttgatatcta aacttgataa
     1741 tgtcttgtta aatccttcag cttctgcccc accacctaat tggctagatg cagattgttt
     1801 agctatttgt ttagctatat caccagtaac cttattcaat atattattcc caagcttgtc
     1861 agctgtatat tgagctaaga aataggaacc aacgaatgcg gggattgata taaataccat
     1921 ctcaattagg aattgaccaa aaatttctaa ttttgatata cctaatgata gtaatactgc
     1981 tatttctttc tttctagcat tcatccataa gaataataat aatgaaacta
//
LOCUS       DACCXM010000225         2017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCXM010000225 DACCXM010000000
VERSION     DACCXM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.235"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..183)
                     /locus_tag="IVY61_002824"
     CDS             complement(<1..183)
                     /locus_tag="IVY61_002824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971874.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(165..515)
                     /locus_tag="IVY61_002825"
     CDS             complement(165..515)
                     /locus_tag="IVY61_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971875.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            complement(508..1833)
                     /locus_tag="IVY61_002826"
     CDS             complement(508..1833)
                     /locus_tag="IVY61_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7971876.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
BASE COUNT          570 a          427 c          312 g          708 t
ORIGIN      
        1 atagccaata tagtattcgt atttatggta tcctgctgca gcttctacat agaaagagaa
       61 gcgttcaaca ccaacctcat ttatcaaacg ctggccataa ccccaaatct cttctgatag
      121 ctgatcaaag gttatatact ttagctcata ttgctggata atgttcattc taatcgaccc
      181 catttattat cctggtaaaa ttcgatatga cgtatttcgt cgtagtgaac tttgtttttt
      241 ccaacgtaga tccccagctc atcactacct aagatcttgc taacaatatc ttctggatag
      301 tatccttcag cgtttcgttc ttctttttga atacatactg atctattttt tatgactgca
      361 gcgttcaata cttccgcaac ctcttcagtc gtcatttgtt cttttgccaa attttgattg
      421 gctcgttgtt cattgtcggc atcaattttt tcagtatgat cagataaata aaaacctgcc
      481 cactttaaac gtccacgatc attataatca atcattttta agccctcctg cgccgcctgc
      541 atggccacca accaatgtac ttctagtaat actacgagcg ccttctaaca tagaagaagc
      601 atgtacaatc gacgtaaaac catatttttg ccgaatcttg tcaataattt tatctaaatt
      661 ttcttcgtca atttgctttt gtggatcact aaataaatcc agctgaagcg aatcagtgta
      721 cacaagcttg ctataggtta ttccaacgtg ccttacttct tgtccttcat aatacttatc
      781 aaacaagaaa agacagtaat tctgcaattc ctttgtatta tctgttatag ggatcttcat
      841 ctggtgtgaa aaacctggtc tcgtttctaa gatcgacgtg ccaatgtata aatggacaca
      901 acctgtttta cagttatgtt gcctgatacg aatagctact tgttccgcca tttcttttac
      961 caccagttcg atttcatttc tcctggtata atctcgtggt aacacttgtg aattaccata
     1021 tgatttttcc tttgtcggtt ctggtgggat cgctatatct gttcgatcga tcccgttcgc
     1081 atgaaaatac agctgtaagc caatcacacc taaatggctc ttaatagtgt atggattcca
     1141 attagctaag tctttaatac tcatgatacc aatttggttg agtctttttt tcattcgaga
     1201 tccaataccc caaaggtctg tcatttcagg aatattccat actttatcag gaacattttg
     1261 atacgtccat tcagcaagga atccatcatt atgttttgct tcattgtcta aagcaagttt
     1321 agctaataag ggattatctc cgacacctac tgtagcaagc aatcctagct cttctttaat
     1381 attctcctgg atcatttgag ccagcttctt tctccgttga cttcttgttc cttcagtcgt
     1441 aaaaagattc attgatctgg tcacttttaa gatcgattca tcgatcgagt aaatcaataa
     1501 atcttcgtct gctacatacc ttctgaaaat attgtttacc tgcatatttc gcttaatata
     1561 gagattcatg cgtggtggaa cgacatgtaa cgttttagga aaaggttgcg gtagatctcg
     1621 tggtcgactg acattagaaa taccgtactt cttctttgca gctggtgaag aagctaaaat
     1681 caaccctgat cctgtattat ccgctcgact catgacgact aattcagtcg ttaatggatc
     1741 taaattcctt tctatacatt cgacacttgc gtaaaagctt ttcatatcga tcaaaaagta
     1801 atcatttact ggctcttttg aataatccat cataaataac actccattct tccacattat
     1861 acaaacatac gttcgttttt gtaaagaaga atcgattaaa tgtacaaaaa aagaagcata
     1921 ggtaagttat ttccctactt cttctgttga tttgtaaaat caacacattg tatcacttta
     1981 ttaaatggat atttactatt agtcttgtga aaatttg
//
LOCUS       DACCXM010000226         2008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCXM010000226 DACCXM010000000
VERSION     DACCXM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.236"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            126..608
                     /locus_tag="IVY61_002827"
     CDS             126..608
                     /locus_tag="IVY61_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971877.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            805..1695
                     /gene="pstS"
                     /locus_tag="IVY61_002828"
     CDS             805..1695
                     /gene="pstS"
                     /locus_tag="IVY61_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP7971878.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          658 a          343 c          410 g          597 t
ORIGIN      
        1 tatttctaga ttaaatgaat aaactctcat gccattatct ctaaaaaaaa agtttttccc
       61 cctaattttt ttagatagaa aattgtatag tatgatgaga gaaaaagtag tttgggggaa
      121 cgttaatgag aagaaaagca gtaatcgatc ggctttattt atttttttgg ttttttccta
      181 tcgttttgct accttttaca tgggtaaatg ctgttgtaca aggtgcggag atattaggcc
      241 tgatccaagg aagcggattt gtggtttcta ggaattttcc agctttgatt gttttctatt
      301 tagtgataag tgtaggtctc ttattaccaa aatctctgat caatcactac aattggcagc
      361 tgctttactt ttcttgctta ttagtattta catctacgtt ttctttgctg cctcgcttga
      421 ttatcggccc taatggtttg agcgagagtg gattgaagga tttagcttat tcctccatgt
      481 atttcttgac gatcaaacca tggttctatt tagtaattgc ctcatttatt cttgcaacat
      541 ttttttactt ctggacagaa atcaggataa aaagtgaacg atctgtatct ataaaaaata
      601 actattgatg gtggatcatc agtaaaaaaa catatgagct gcgtaaaatg catgtttctc
      661 atatgctttt tttattatta tgtaaagaat gcgaaaaacg tttacatgat ttttacataa
      721 cttaactata tttttacagc aaccatacga gggatttaca gtcgactgat aaactttagt
      781 tgtaaataag aaaggagcaa taaaatgaaa aaagtattgt ttactctagg tatgatggga
      841 attcttttag ccggatgcgg ctcaggatca ggaaatacag atggctctgc cacacaaaat
      901 aattcaagtg actcaaatga acaagtaaaa atcgttgcgg taggttcgac agcattacag
      961 ccgttagtcg atgcagcaca agaaagcttt gtacaagaaa atccaaatta tcaaatctct
     1021 gtacaaggag gaggaagcgg aactggtttg agccaagtcg aagctggagc agttactatc
     1081 ggaaactctg atgtatttgc agaagaaaga gacggagttg atgcttcaaa attagtggat
     1141 cataaagtag cagtggtcgg gatggcacca atcgtcaata aagatacaga tgtcaaagat
     1201 atcaccaaac aggaattgat tgatatcttt acaggaaaaa tcactaactg gaaggaagtt
     1261 ggcgggaaag atcaaaaaat caacgtcgtg aaccgtgcaa acggcagcgg tacccgcgcg
     1321 acattcgaaa aatggggact ggatggtgct acccctgtgc agtcccaaga acaagattca
     1381 tcaggaacag ttcggcagct ggtaagtcaa acaccaggag caatcagcta tttagctttc
     1441 tcatatctcg atgattccac acaagcacta agtatcgacg gtgtagaacc aaaagaagag
     1501 aatgtagcag ataacagctg gggaatctgg tcatatgaac atatgtacac gaacggaaaa
     1561 ccttcccctg aagtccaaaa attcttagac tacatgatga cagaagaaat ccaagaagga
     1621 cctgtcaaag aattgggcta tttaccaatc acaatgatgg aagtagaacg tgatcacgaa
     1681 ggaaatatca aataactagc agataaacat gccacggtag tcgttactat taaggaaaaa
     1741 tcaactatgt ttccccacat atacattttt cctttaattg tgaaaaatag ttcctcatat
     1801 tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt attcgaaaaa
     1861 aagagctacg acatagaact gtcgtaactt tttttggctc ttcgtcaaat cgtgttggtg
     1921 aagacaaatt tgaataaaac ttagaccgat atgcggcgcg ttctgtgcgc cgcatatttt
     1981 tttagttttc aaagatcaga ccttgttg
//
LOCUS       DACCXM010000227         1957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCXM010000227 DACCXM010000000
VERSION     DACCXM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.237"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            291..857
                     /gene="amaP"
                     /locus_tag="IVY61_002829"
     CDS             291..857
                     /gene="amaP"
                     /locus_tag="IVY61_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7971879.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            870..1058
                     /locus_tag="IVY61_002830"
     CDS             870..1058
                     /locus_tag="IVY61_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7971880.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1071..1628
                     /locus_tag="IVY61_002831"
     CDS             1071..1628
                     /locus_tag="IVY61_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7971881.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1660..1902
                     /locus_tag="IVY61_002832"
     CDS             1660..1902
                     /locus_tag="IVY61_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7971882.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          722 a          302 c          401 g          532 t
ORIGIN      
        1 ctccgaaaaa atttaataaa taaatagaat cttgtaataa taactgagca taatctttgc
       61 aatttttaat cttgaataat atcctttaag tatagccagc aatcaaaagt aaatagagaa
      121 taagtaagca attgttattt cagtatcaga ctggcaataa aacgccgtta tgatccagca
      181 atgtttggta gcgctttatt tactgatgct atgctaactg ttcgctatct atactgtgat
      241 tgtcggtaga gttatttaca gggtaaaaat aagtttatag gaggtggaaa atgaataagg
      301 ggttaaaaat aatcggtgtt atcatgagtt tactgctact ttcagtattt ctagcgattg
      361 cgttgatcaa tgcgccgtat attatgcctg aacaattgga acgatttcgc ttctttactt
      421 taacgaatta ctacatgcaa caatatattt tctgggcagc tgtagtattt gccgttcttg
      481 ctattatcat cttgctagtc gttcttttct atccaaagtc aagaggaact ttcgttatga
      541 aacgggaaga tggcaaacta acaatcgaca aaaaagcgat cgaagggttg gtacgttccc
      601 atttacacga agaagagttc attcattcac ctaaagtacg gatacgttca acgaaaaatc
      661 gaatccatat ccatgtcaac ggagacttga aacgaacttc ttcattagta gggaaaacag
      721 gtgtattgat gcaagatatc gaagaagaag taactaaagt actcggaaca aaagaaacgg
      781 tcaaagtggc agtcacatac agcggttatg aagaacaaga agatacaaga gactacaagc
      841 actctcgagt ggaataggag ggccaaataa tgaaagaact attttcagca tacaagctcc
      901 ctattatctt tgggggtctt ggactactgc tagctgtttt atggttaacg attggatttt
      961 tcaagacatt gttgatcata atcatgacag caattggcgc aggaatcggt ttttatttga
     1021 aacagtcagg aatagttgat caatttttta aatcatagga ggaaacaaag atggaaaaca
     1081 aaaacatgaa tacaacaaat ggatcagttt tggcaaatga aaaaggagaa aaccgcatga
     1141 tgaatggtga attgacttat gaagacaaag tcatccaaaa aatcatcggt tatgcattga
     1201 aagatgtaga tggtctattg acattagatg gcggattttt ctcaaatatg gctgaaaaat
     1261 tagtcaacac agataacgta acagcaggta tcaatacaga agtaggtaag aaacaagtag
     1321 ctgtggatct agatgtgatc gtagaatacg gtaaagacat cgaagatatt tacaaccaaa
     1381 tcaaagaatt gatcagtact gaagtaaaca atatgacaca tttagatgtt atcgaagtga
     1441 atgtgaatgt tgcagacatc aaaacacaag aagaatacca aaaagatcaa gaaacagtac
     1501 aagacaaagt aacagaagca gcaaaatcca caggacaatt tgcttcacgc caaacagata
     1561 aagcaaaatc tgctgtaggc aaaggtgcac aaaaagtcaa agaaaataac gaaccacgtg
     1621 tacaataaga agtaagaagt cagttaggag gaatttaaaa tgggatttat ttgggcatta
     1681 atcgtcggcg gtatcattgg agcaattgca ggagcaatca cgaataaagg atcatcaatg
     1741 gggatcatcg caaacatcat tgctggttta gtaggttcag caattggaca agctttacta
     1801 ggaacttggg gaccaacaat tgcaggaatg gctatcattc catcattgat cggtgcaatc
     1861 attttagtat tagtcgtttc attctttgca cgtaaattat aagcaaattg aagaaaaact
     1921 tatgttgaaa tgaagaacaa atttgagaac cgtagcg
//
LOCUS       DACCXM010000228         1941 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCXM010000228 DACCXM010000000
VERSION     DACCXM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.238"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            89..1555
                     /locus_tag="IVY61_002833"
     CDS             89..1555
                     /locus_tag="IVY61_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7971883.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          599 a          332 c          397 g          613 t
ORIGIN      
        1 acataaaata acactgtctt gtaagtttgt taaaaattga atatgattac atctggataa
       61 caaattttta tcataaagga gagaacgaat gaacaacaaa caattacgct ggtttactgt
      121 tgcgttaatt gcatttaaca tggtttgggg catggggaat gtagttaata actatgctca
      181 gcaaggagta tcagttgtca cctcttggct attgattttg gccatttatt ttattccata
      241 cgcattgatc gttggacaat taggatcagc cttcaaggat agtaaaggag gcgtcagttc
      301 atgggtcgaa aatacgacat ctaataaacg actggcatac tatgccgctt ggacttactg
      361 ggtcgtccat attccttatt tggcacaaaa gcctcaagca attctgattg ctggtggttg
      421 ggcagtccaa ggaaatggaa atatcgtaaa tacgatgtca gtacaaatgg tagcagttat
      481 ttcacttatt atatttcttg cctttttata tttatcaaca aaaggacttt ctactttgaa
      541 agtgattggc ggactggccg gaacagcgat gttcgtcatg tcgatcttat ttattctttt
      601 agctgtaacg gcaccgttca tcaatccaac gatggaattt gccacaccac atatgaatca
      661 agtgaaaaca tatattccaa catttgattt cagttatttc acaaccgttt cgatgctagt
      721 atttgcagtt ggaggggcgg aaaaaatatc cccttatgtc aactctacaa gaaatcctgc
      781 aaaggaattt cctaaaggga tgatcttctt agcagcaatg gtagggattt gtgctgtatt
      841 aggctcagtc gcaatgggta tgctgtttgc tagtgataat attcctaaag acttgatggc
      901 aaatggggca tacagtgctt ttcaaatttt aggtaattac tatggtatcg gtaactcatt
      961 gatgatcatt tacgcattga caaacatgat tggacaaatc tccgcattag ccttttcaat
     1021 tgatgcacca ctaaaaatct tgttagcaga tgcggatcca gaatttgtac caaactggct
     1081 acgtaaacgt aatcgtaaag gtacattggt gaatggttat cttctgacag gtatactagt
     1141 aggaatcatt attttgttgc cgatttttgg tatcaaagat atgaatgaat tagtaaaatg
     1201 gttgacgaac ttgaactcag ttgtcatgcc aatgcgttac ctatgggtgt tcttggcata
     1261 tatgatgttg aataaagctt ataagaaatt caattctgaa tataaatttg ttaaaaatcc
     1321 aaaaatcggt tttatctttg gtgcttggtg ctttgccttc actgcgtttg catgtatcct
     1381 aggaatggtg ccaaaagtag attatactgc tgatcctaaa gcatggtggt tccaattatt
     1441 tacgaatatc atcacaccaa tcgtcttgat cttattaggt atgatcttgc cagcaattgc
     1501 tcgtcgagac aaaaacaaag aaatcactga accagaaaca gtggttcagc cttaaaatag
     1561 aaatacttgt tacaagaggt tgtgacagaa gtggtcagct tcaagaaata agaaggaatt
     1621 tacgaaaatt gcttctcaaa tttttgtgaa gtttcggctt gtttttcgaa gaagctactt
     1681 ctggaacacc gtgaatcaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacag
     1741 ccaaacaaaa atagcccctc atatttttcg tttggctggg cttattaccg cagatgcaag
     1801 ctttggaaca ccgtttaagg aagaggttgt aaaatcagcg atttgacctg agtattagag
     1861 aaacaaacgg aaaaatagct cctcatattt tgccgctttt tgagctaatg ctgaaggtca
     1921 gctgattgaa caccatttat t
//
LOCUS       DACCXM010000229         1886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCXM010000229 DACCXM010000000
VERSION     DACCXM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.239"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(330..785)
                     /gene="tnpA"
                     /locus_tag="IVY61_002834"
     CDS             complement(330..785)
                     /gene="tnpA"
                     /locus_tag="IVY61_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP7971884.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1028..1886
                     /locus_tag="IVY61_002835"
                     /pseudo
     CDS             1028..1886
                     /locus_tag="IVY61_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          599 a          366 c          354 g          567 t
ORIGIN      
        1 taactcaaaa atacttccaa aggtgttcga taatccagtg attttctagg aagattattt
       61 ctgcgccagc attttctaat gtttgaattt gtgttttgta atcttggttg cttgtgctaa
      121 ttcttgaata gccgtaaatt gccaaaattt catcaccctt tctggacata agtttctgac
      181 accagttaaa atcaagtcta tcagtaaccc atacacccgt gaggacgggt gtatttttaa
      241 tccccctaga aggggtcgac actcgctgcc ccttaaaagg ggatttttaa aagaccgcca
      301 actgctcatc ttcactcact tacttctctt tattttttaa atggttctac gtactctctt
      361 ttacttataa tatatcgaat gcgatcatca gcttcttgtt cacggatgta tttttcattt
      421 gtcttttgat tcaatccgac tgtactcaca tagtatctct tcgaccaaaa ggatttgttt
      481 ccgtatttat atttgagatt tgcgtgctgc tcgtggatta gtaccgcact acgacctttc
      541 agatacccca taaaactgga tatacatatt tttggtggta ctcgaacaag catgtgaata
      601 tgatctagca ttgcatgtgc ttctatgatt tccacatctt tcatttcgca caacttccta
      661 agtatttttc caagatcttt tcgtaattgc ccatagatta cttttcgttg atattttggt
      721 gtaaaaataa tatgatactt acaattccaa cgagtatatg ctaaactttt atcgtcattt
      781 gacatgaaaa aactcctttc gatctgatga gcggttggta gccactcact agtatatcga
      841 aaggagtttt tatcgctaaa gatacttctt ccacgcgtag aaacgcgcgg ttttcttgtt
      901 tcgcacgctt tacgcacaaa actgactgaa gtcattaaga aaataaaagg gtctaaaact
      961 tttaagtttt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt ggaggtcggt
     1021 attttttatg gctaagtata cttttgaact taaattaaag atcgttcatg attacttgga
     1081 tggtaaaggc ggaagtgatt atctggcaaa gaaatattcc attaaggctc catcacaagt
     1141 gaagcgctgg atcaatgcct atcaagaatt tggtgaagag ggtctagtaa gaaagcgaca
     1201 aaagaaaaaa tattctgttc aatttaagct ggatgcgata gagttatatc taacaagtga
     1261 gctgtcctat cgtgaagttg ccaacacact taacatgaac aaccctaatt tgatcgctag
     1321 ttggatgcgt caattccgtg agggtgggat cgatggactc tcaaaaacga aaggatgtcc
     1381 acctatcttg tctaagaaaa atgaacccaa aaataagaaa aaatctacaa ctaaagcaac
     1441 gtctaaagaa cgtgaacgca tcgaggagtt ggagaaacga gtccgttccc ttcaaattga
     1501 aaatgccttt ttaaaagaat tgaggaagct gcgaaaacag gaagcccaac aacgacgaat
     1561 gaagcaatcg cacgaatcat cgcaagcctc cgaggaccgt ttaaattagt tgagcttcta
     1621 gaaactttaa agttccctaa ggcaatcttt atgtactggc aaaaacgatt ggaccgtaaa
     1681 aatccagatc aagagattga agcggaaatg cttaaagttc gggagaagca caaggattat
     1741 ggctgtttac ggatgaccaa tgaactaaga aaccgcggtt tctctatcaa taaaaagaaa
     1801 gttcagcgcc tgatcaagaa gctaggaata aaagtaacga catacacacg taaatcacgg
     1861 cgctacaatt cttatcgagg acaagt
//
LOCUS       DACCXM010000230         1859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCXM010000230 DACCXM010000000
VERSION     DACCXM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.240"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="IVY61_002836"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="IVY61_002836"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7971885.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DACCXM010000231         1822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCXM010000231 DACCXM010000000
VERSION     DACCXM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.241"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            270..677
                     /locus_tag="IVY61_002837"
     CDS             270..677
                     /locus_tag="IVY61_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971886.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            691..1092
                     /locus_tag="IVY61_002838"
     CDS             691..1092
                     /locus_tag="IVY61_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971887.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1094..1465
                     /locus_tag="IVY61_002839"
     CDS             1094..1465
                     /locus_tag="IVY61_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971888.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1501..1803)
                     /locus_tag="IVY61_002840"
     CDS             complement(1501..1803)
                     /locus_tag="IVY61_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971889.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          680 a          246 c          302 g          594 t
ORIGIN      
        1 agttgttgat atagttgcta atattgttag ctaatttgtt agtaaaactg tatacgaatt
       61 tagatagtaa aaaaacagtt tgttccttat atagaggcac ttttactact gattaaatgt
      121 cagatatgct tacaaatata cttataaata tgcttacaaa attagctaat atttccgttg
      181 acaaagcttt gttactattg ttaaactaaa ttgactagca aaagtgaaac ataaatttat
      241 taaataaatg taaaaattag gagaaatcaa tgtcttatag agtacaatta ataatttcag
      301 aagatgtaga aagtcagcaa tttggcacta atgttattaa taaagtaatt aatccagctc
      361 tttctattaa tgcgccttta attcctactg ctttatcatt ttctgtaaca gctgtggtgt
      421 ctgagataga ggatacagaa aagataaaaa tagtagaaat tgaagtttta aacaagaatg
      481 aaaaacaaat tttttctacg ggtgaagtat cggtaagctt gcctcctcaa gttaacgata
      541 taaactttaa tataaacgcc agaaatgtct tggttgaaga agcaggagag cattatgctg
      601 ttgttaaatt taatggaact gagattggaa ggcaaatctt tgatatcaag gtcaacaaac
      661 cagtggaaaa aaattaagga gacgatacgg atgcttgata tagtaccatc aaatacacat
      721 cggagtggta gcttaataaa ttttgctgcg atttttgctt gcgcatcggt aattctagca
      781 tctccagttg ttgtggatgc aagttctact ccgaaaacta aaaatgataa tcaacttgta
      841 attactacaa aaaatgagat taacacaatt tcaagtaatg atggcaaaat ttttgatgtg
      901 ataaatacgg ttttaaaaag aaaagctgaa atcaatataa aatatgatga agatttaaac
      961 ttgtatttct ttgttatcaa aacaacatct gaattgtttt cctcagatta cgatgttttg
     1021 gatactctcg ataatgtatt atctgactat aaatatatgg ggaaatctgt agtagcaact
     1081 ttgggggaat aaaatgtttg actttaatga atatttatct atttccaaac aaattgaatc
     1141 agatgataaa tataattcta aagaatctta tagaagaact gctgtttcaa gagcatacta
     1201 cagtgctttt aaaaaatctg atgagtattt gaaagaaaat tatgatatta tttataatgg
     1261 aagtagcgga aaaggaagcc accaaaccgt ttggaatttg ttttcaacag ttaaggaatt
     1321 gaatacctta ggaatacata atagtgggta tagaatgtta gagaaaagaa aatgtgctga
     1381 ctatattcct aatgaaacca ttacgaaaac agatatggcg ctgatgaatc gagaagcaga
     1441 aaaaataata aacaaactca cctaatgaat ttcaagcgag tttgtttgtt atttttttat
     1501 ttaatctttg tatctttctg gatcaacgaa agtatacttt atatagtcat aacgccgatg
     1561 atcgctacgt gcgtccggca cgtcagtcac gatatcaaac aaaaagtata cacccttctt
     1621 cattctagtt ttcgcagcag gaattttgaa atagttctta ttagaatagt agagattgat
     1681 taataagcta tcttcgattg ctaaaaagaa aacttctgaa tcccacacct tataaaaatc
     1741 tttgacaaat ctattcgaag gatcgaattt aaaccataat tgtgtttttc cttccattaa
     1801 catataaagt tcacctcaaa aa
//
LOCUS       DACCXM010000232         1709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCXM010000232 DACCXM010000000
VERSION     DACCXM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.242"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            135..731
                     /locus_tag="IVY61_002841"
     CDS             135..731
                     /locus_tag="IVY61_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7971890.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(789..1271)
                     /locus_tag="IVY61_002842"
     CDS             complement(789..1271)
                     /locus_tag="IVY61_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971891.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1377..1574)
                     /locus_tag="IVY61_002843"
     CDS             complement(1377..1574)
                     /locus_tag="IVY61_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971892.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          566 a          348 c          264 g          531 t
ORIGIN      
        1 ttattaaaat tgttttttga acttcgctta ttttttgtga gcatatctgt ttacttactt
       61 tttgtaaggt gctatacttc atttcgtaat caattaaaaa aatgcaaaac gcaaatcaaa
      121 ggagaaattt tattatgaca acttttactg acactttgaa aaaccgccgt tcaatctatc
      181 atctaggacg taatgtttca ctttctaatg aagaattaac aactttgatc aaagaagcaa
      241 ttaaagaaag cccaactgct ttcaatgctc aatcaacacg cgcagttatc cttttcggag
      301 atgcacatga aaaactttgg gaaatcactg aagaagcact tcgcccattg actccagcag
      361 aagctttccc aaatacacaa aacaaattag ctggtttcaa aaatggttat ggtacagtat
      421 tattcttcaa agatactgac gtggtaaaag gtctacaaga acaatttgaa ttatatgcag
      481 acaacttccc agattggtca gaacaatcaa acggaatcgc aacagctaat acttgggtag
      541 cattagttga taaaggattg ggcgcaaact tgcaacatta caacccagtt attgacgaag
      601 cagttgcaaa agaatggaat atcccttcaa actggaaact acgttcgcaa ttagtattcg
      661 gttcaccaga aacaccggct gaggaaaaag aatacatgaa cgatgccgat cgcttccgcg
      721 tatttggata attttatttg aaataaaaaa cagaggttgt gacaaacatc ttgtcgcaac
      781 ctctgttttt attctggatc aatcataata tcaatctctg cctcatcgtt taagcgttgg
      841 gacaaaaatg gaactaattc ttctaatgat gcagaaaatt tcagcatcgg tcctcgccat
      901 ccgtttcgga aaaaattcat ttttacgatt ttagaacctt ttggctgata taatacaatt
      961 ttattgatct cagaaaaagg aacacctttt ttgtcaaagc tattcaaaac aagtccttct
     1021 tctgtgattc ctctactgtc caacaaaaaa ctaataaaaa tcattgctga caagatccct
     1081 tttgccaatt catctgctgt aggatgactg atgagtgtat accaaatgac tagtgacact
     1141 aagaagggta tcaccaagta gacccatcga tttcgcacag tccgataaat aatttctttt
     1201 cgaaccagcc aaagaaataa aaacatcaca acaacgaata aaaacaatag aaccgtaaag
     1261 aatgttacca tgacaactcg cctcgctttt tcttctccag aaaataggaa aagaatactg
     1321 atttaccttc tcaaagaaat tggacttgca gcacgccgca cgtccaattg ttttctttat
     1381 tcaaatgtat catattttgc aacaaccatg cttgcttttt catctaataa ttcttggtcc
     1441 tctgcagcac attgggcaac tttgaccacg gcgcagtttg tcatttttga tacaacttca
     1501 ccaatgaccg tttttgtaaa accaattggt ttgcatgcat attgttctcc taaattaatc
     1561 atttcttccg tcatcaaaat cctcctaaac tgctggaact tttctccaca ttaccactgc
     1621 tatttctttg gagatttaac tcattgattt ttatttacag ttaaaaatca accttacagg
     1681 cattatagcg tactgttttc gttttcaca
//
LOCUS       DACCXM010000233         1655 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCXM010000233 DACCXM010000000
VERSION     DACCXM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.243"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            81..1604
                     /locus_tag="IVY61_002844"
     CDS             81..1604
                     /locus_tag="IVY61_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7971893.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          608 a          269 c          339 g          439 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaa
//
LOCUS       DACCXM010000234         1643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCXM010000234 DACCXM010000000
VERSION     DACCXM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.244"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..409
                     /locus_tag="IVY61_002845"
     CDS             <1..409
                     /locus_tag="IVY61_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971894.1"
                     /translation="ILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELTPLDRN
                     GIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEK
                     RNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            419..622
                     /locus_tag="IVY61_002846"
     CDS             419..622
                     /locus_tag="IVY61_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971895.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            833..1417
                     /locus_tag="IVY61_002847"
     CDS             833..1417
                     /locus_tag="IVY61_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7971896.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          577 a          238 c          300 g          528 t
ORIGIN      
        1 tattttatct ttaggtattt taactattgg aattagtgta actggaacta caattcctac
       61 atttgctgat agtacaaatg aaccaactac tgtagtagat aataaccaag aattaactcc
      121 tttagatagg aacggaatag ccattggtaa cccaactaac gacttaactg ttactgaaaa
      181 ttatttaata gtttctccat tacttgctcg agcagcttct aaaacgatac gagtgacagg
      241 aacgatgatt tttgataaag ttggttggcc ttctagacct cctcaatcac ggaattggaa
      301 agaaaaaaga aatggttatt ggtactatgg aaaattgtat ttacaaagtt ataaatcagc
      361 tggaaacaat tggattgcga cttattcagg aaccttaact agaaagtagg gattttttat
      421 gccagaattt gtgaacacac caaattttta tcaagatagc gatattctaa gtaaaattaa
      481 tttaccgaaa gaagaatcag ctttacaagt tcagcgaact ttacaggaga cacaaaaaat
      541 tcaaaaatca atttctaatc tagaagattc tgaagaaaaa agaaaaaaaa gagaccaaaa
      601 aacatatcaa aaagaaaaat aatgatttta aaaaaagagt gtctagtcga cattcttttt
      661 ttctaattga aagtgttcat aaaatcaccc atttctggac actttctttt tttgctttat
      721 ttttcaaaaa aacaattcat ttcatgttca taaacccata acaatatcta tgttcagtta
      781 aactatatgg ataaagttat gttacaataa aattaatgaa ggagtgattt ttttgaaaat
      841 aggatacgcc cgggtatcta ctggtttaca aaatttggat ttacaagaag acagcctaaa
      901 taaatttggt tgtgaaaaaa tttttactga tcatatgagt ggggcaaaaa gtaatcgacc
      961 aggattagaa atggccattg attttgttcg ctctggcgat acacttgtcg tttggcgctt
     1021 agatcgttta ggtagaaata tggaagattt gatttcaatc gtcaatcgac tgaatgagcg
     1081 tggtgtgagt tttcatagta ttcaagaaaa tattacgatg gacaaatcaa gttctacagg
     1141 acagctcatg tttcatttgt ttgcggcatt tgcagaattt gagcgtaatt tgatcctgga
     1201 acgatccgca gcaggacgag cagctgctcg tgcaagagga cggtttggtg gtcgtcctga
     1261 aaaattgtct gaacaagatt tggaattact gaagacttta gtggatagcg gtacacctat
     1321 taagactatt gcggaacggt ggaacgtctc aagaaccacc atttatcgtt atttggcaaa
     1381 aatggaggag aaaaatggaa agacaaattc tcattagttt ttgtcattta atgctgaaaa
     1441 tttttgccat ttatctagaa aagtgacaat agtatgagaa ttttttgtaa gtgtctactg
     1501 ataacttatt ctcaaatgtt gtaaggattt tattcatttg ttaatattaa agaacggaac
     1561 tccaaaaaag tccgtactct aatttgcagt ctctcactct tttagagtga gagtttttat
     1621 tagattttca aattaattag taa
//
LOCUS       DACCXM010000235         1620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCXM010000235 DACCXM010000000
VERSION     DACCXM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.245"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1477
                     /locus_tag="IVY61_002848"
     CDS             <1..1477
                     /locus_tag="IVY61_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971897.1"
                     /translation="KKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARVKGLS
                     SIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNVCTIM
                     LMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYYAYAL
                     IYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRLIVEL
                     SKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDLTTFT
                     VGIIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMIQFPW
                     RILGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLLLLAVSAATKANYEHKVVTQR
                     DHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQVMDSK
                     IVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNRTSVD"
BASE COUNT          504 a          265 c          290 g          561 t
ORIGIN      
        1 gaaaaaaata atattgtcca ttctcctagc aattattgct ggactttatt caatcaatta
       61 ttttagaaat gttcatacta tttctacatc gggggatatt gccttccatt ttgctagagt
      121 caaagggtta tcttcaattt tttctggtcc aatcaatttt acgacgttta atcattatgg
      181 gagcggagtg aattattttt atccctactt gacctttttt ccggcagtca ttttttattg
      241 gataagtaat aatttaatcg ttagttatat cttatatgtt tggctattga atgtttgtac
      301 tatcatgctg atgtttcatt atggactaaa atttttaaaa agaatcgatg cggcttttat
      361 atttagctgt ttatatacat tttatgggta ccgtacgatt gatatttatc atcgttctgc
      421 tattgctgaa gcgattgcac ttacggttat accgatagtt atgtattatg cttacgcact
      481 gatttatgag aaaaagccat gtgcaattcc attagcagtc agtatgagct tactcattta
      541 tacacatgtt ctttccactt ttatgagtgt aatagccgtt ggcttactag tcttaggcgg
      601 ctttgtagta agtgaaacaa gaaaaaaaga aagcatccgg ttgattgtgg agttgtcaaa
      661 ggcagtagga atgacgattc ttttaacagc atacttttgg tatccgatgt tccgacaaat
      721 catggttcaa tcgattaatg agccagataa aacgacctta caacaccaag cattaaatgt
      781 ctcggatagt ttgattggtg cgatgaataa tgacctgaca acctttacgg taggaatcat
      841 tggtattatt tctttgatct gtccactctt cttttttaaa ggatttaatc gcaaagaaaa
      901 agtgatttac cttagtgcgg tagtctcttg gctcttatcc accaaatttt ttccatggtc
      961 tttattacaa aatacaccca ttcaaatgat tcaatttcct tggcgaatct taggctttca
     1021 agtactgttt ggctcgttga ttttatcgtt cgttttcatg aagagcacat cagctaagca
     1081 aagaaaagta gttaatataa cggcaattgt tttattactg ttggctgtat ctgctgcaac
     1141 aaaagcaaac tatgaacata aagttgtcac tcagagagat cacttggtaa tgaatcaaga
     1201 aaatcttatt cgttacacga cactccttcc cggaggactt tatgactatg cacccacgga
     1261 agcattacaa tacaaagaac atttgcagaa tcatgaagta agaattggca aggaatggca
     1321 tactatgcct tatcaagtga tggattcgaa aatagtctat acagtacatg aatctaaagg
     1381 acaaaaaata acgcttcctg tatttgatta ttggggaaca gtcgcaaaag taaatggaca
     1441 aaaaagagag attgataata ggactagtgt cgattgaagg aacacaagga gaaacaatca
     1501 ttgaagtttc ttccatttat cctccaacta tgaagttagc ttttttaatt agtttatgta
     1561 gttacctctt tcttttaatt agttttctta tcaggaagtt acaacatata aaaataatga
//
LOCUS       DACCXM010000236         1589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCXM010000236 DACCXM010000000
VERSION     DACCXM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.246"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            190..453
                     /locus_tag="IVY61_002849"
     CDS             190..453
                     /locus_tag="IVY61_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7971898.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            453..725
                     /locus_tag="IVY61_002850"
     CDS             453..725
                     /locus_tag="IVY61_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7971899.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            802..1131
                     /locus_tag="IVY61_002851"
     CDS             802..1131
                     /locus_tag="IVY61_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7971900.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1121..1407
                     /locus_tag="IVY61_002852"
                     /pseudo
     CDS             1121..1407
                     /locus_tag="IVY61_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          582 a          203 c          303 g          501 t
ORIGIN      
        1 ttttttgttg atttaccaac atgtttagac attttcctct aggaaaaaat taactagcac
       61 cacgggtata aaggaaacgg ataacacttt acaatgctgt tttggtactc caatttaaac
      121 aaaacaatcg aatttacgta cttaattaag tacgttagaa tggagatata aattagaggg
      181 ggagtagata tggcaaaaac agtcttaaat cctagctcag cacggaaaaa tttttataag
      241 ttgttgaaag atgtaaatga gaatcatacg gaaattgaaa tcattagtga gcgcagtgaa
      301 aataatgcgg tgcttattgg tttggatgat tggaaagcaa tcaaagaaac gctattactt
      361 gagcaaatag gcactttggg tgttgttcgt aagcgagaaa aagatgatag tggatttact
      421 aatgttgatg aaattgattg ggataactta taatgaataa ctattctgtg atgattaaaa
      481 actcggctaa agctgattta aagaaaatca aacagtctaa tttgaaacct caatttgaga
      541 aggttattca gactttaaag gaagatccct atctccctac ccaatcattt gagaagttaa
      601 aacctacaca tgaaggtagg tattctagaa gattgaatag acaacaccgg gttgtttata
      661 aggtagatga agatgaacac gtggttgaaa tttattcagc aaggacgcat tacgagtcac
      721 agtaattact tgtaaataca ttatctcgtg gtataatgta tttactaagt aattacatac
      781 tgttagaaat gagggattta aatgattaca aaaacaagaa aacaaggcaa ttcaatcatg
      841 ttgacagtac ctaaagattt taatgttcct aatggagtcg aagttgaagc aaagttggta
      901 gagaatggta ttctttatga atttgttgaa ccacaaaaag aattttatga ttttagcgaa
      961 gatattttgt ctgatataat tgcagaaggc tacgataaag atgagattct tgtagaattt
     1021 aagaatagaa aaaacaaaat gcattcttcc tttagagata ttgctgaaga tacacttact
     1081 aattctaaag taatgactaa agaggagttg gcaacagaaa ttggcttatg aaatcgttcc
     1141 ttcaaaacat gtcattaagt atttaaaaag ctaaaagaga agccattgaa agaaaaattt
     1201 ttgaatttaa tttatgatga aattgcgatt gatccatata agggtgaaca aaaaactggg
     1261 gatttatcag gtatttggtc tagtgggttc aaatatgcgg gcacaacata tagagtagct
     1321 tacgagatta tagaaaacaa ggttattcca gtattgttat gtggaacgca tgaaaacttt
     1381 tatgaacagt tgaaaaaatt aatctagtga tttgttgtcg ttcttaagaa ggacggcttc
     1441 ttttttatcg aaaatctgat tcgtctacat gactaaagtc gcgagtattc tctgcttctc
     1501 gataaattaa ataacgtcat tactttgagg aaagctataa atcaatatat ttcagctttt
     1561 ccttatttat gtactacctt ttacttatt
//
LOCUS       DACCXM010000237         1539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCXM010000237 DACCXM010000000
VERSION     DACCXM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.247"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            25..1491
                     /locus_tag="IVY61_002853"
     CDS             25..1491
                     /locus_tag="IVY61_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP7971901.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          423 a          289 c          326 g          501 t
ORIGIN      
        1 atttaaagaa ttggggtata gattatggaa aaacagcaag acaagggctt cttcgggcag
       61 ccgaagggat tgtcaacttt gttcttcact gagatgtggg aacgatttag ttattacggt
      121 atgcgtgcat tacttatcta ttatatgtac gatacagttg ccaacggggg acttggatta
      181 ccacgagcaa cagcattagc tatcatgtcg atctacggat ctttgatcta catgtctagt
      241 atcgtcggcg gatgggtatc tgaccgagtg ttaggagcaa aacgcactgt attcttcggt
      301 ggagttttca ttatgcttgg ccacatcgtc ttggctttac catttggtgt aagtacattg
      361 tttgtttcga tggcattgat tattatcgga accgcctttt tgaaaccaaa tgtttctggc
      421 atggtcggac atctttattc aaagactgac ttgcgaagag atgcaggatt ttcgattttt
      481 tacatgggtg tcaacttagg ggcattatta gcaccactaa tcgttggtac aatcggtcaa
      541 gaatataact atcatttagg gttctcttta gctgctattg ggatgttctt tggattatta
      601 caatacatca tccaaggacg caagacatta gacggagtgg gtacaacacc gccgaatcct
      661 ttgacaactg aagaacgtaa aggattcatc cgtaacttga tcatcgcact tgttgttatc
      721 gctgcagtat tcggcggggc acaactaact ggtcatctga caattgactt ttttgtcaat
      781 acaatcagtg tattggggat tatcttaccg ttgtattact ttatcaaaat gctgacttca
      841 aaagatgtaa ctgctgaaga aaaaccaaga gtatgggctt atatcccatt attccttgca
      901 gcaatcattt tctggtcatt acaagaacaa ggttcatcta tcttagcttt atttgctagt
      961 gaacgtacac aatctacgct atttggtgta acgatccaag caagctggta tcaatcattg
     1021 aatccaattt tcatctttat tctgacacca gtattcgtaa cgatgtggac gaaacttggt
     1081 gaacgtcaac catctacagt agtgaaattc tctcttggac ttttatttgc aggattatcg
     1141 tacttgctat tgatgttgcc gggtatgctt tatggtacaa gtggaaaagt aagtccattg
     1201 tggttgttgg gaagtttctt catcgtggtt attgctgaat tgtgtctttc accagttggt
     1261 ttgtctgtga caacaaaatt agcaccacga gcatttgaat cacaaacaat cgctatttgg
     1321 ttcttagctg atgcttcttc acaagcaatc aatgcacaaa ttgcacgctt ctacacaccg
     1381 ggaacagaat ctgcttactt cggtattatc ggtgcagtag caatcgtcgg tggtatcttg
     1441 ctatttactg tgaaagaacc aatcaaaaaa ctaatgggct ctattcatta atcaatgaac
     1501 ataaaaacag gaattgctta tttgcagcag ttcctgttt
//
LOCUS       DACCXM010000238         1509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCXM010000238 DACCXM010000000
VERSION     DACCXM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.248"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(86..158)
                     /locus_tag="IVY61_002854"
     tRNA            complement(86..158)
                     /locus_tag="IVY61_002854"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Gln,seq:ctg)
     gene            complement(204..632)
                     /locus_tag="IVY61_002855"
     CDS             complement(204..632)
                     /locus_tag="IVY61_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971902.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(692..1450)
                     /locus_tag="IVY61_002856"
     CDS             complement(692..1450)
                     /locus_tag="IVY61_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP7971903.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          437 a          285 c          280 g          507 t
ORIGIN      
        1 acaagtagta aaaataatac aataatcccc ctgcaaaaag attgttcttt tacagggggt
       61 tcattttttc agcaagcaca tcaaattggg atggcagggt tcgaacctac aattgcttga
      121 ttcagagtca agtgccttac cagtttggct acatcccatt tattcaacaa aataatcata
      181 gcactttcct aaaaaaacgc aagttatttt tcttgatcgg atagttgttg ctttactcgt
      241 aaaaggcggc gtactagttt cttttctaat ccagcaggaa gggcaatcag ttctgctact
      301 gacaagacgg tgtccaataa ctgatattct ttgacaggtt cattcataat atgatagatg
      361 tttgctaaac gttcaatata ttcaaagcga gtatactctg ttcgagcaat tagaaatttc
      421 aataaatcgg tggctttctc gtactcttga tctgcgtaat aaacaagaga ttgacgatac
      481 aatttgattt ccagttcatt gagggtagca ggtctgcgac gctcgttcca ctcattaata
      541 gagactttac cttgaacatt tcccttgcag tcacggacaa aaataatcgg aatttcattt
      601 ggatccattt ttttatgtgt gaatttttcc attatctacg ctcccttcaa aggtttggta
      661 tcttgagtat aagggatttt tgtttgaatt actagctttt ctccaaagtt agcatctgtc
      721 cacccattgc agtgaaaatc gccgaaaaat cgttgatcgg tactgctcga ttttttacac
      781 catagggatc gttgacataa atattttctt tgtcatatcc agtgactaca caagcatgaa
      841 caagaggagt gactcttact tctccattat ttgtggtcca agtcataaaa tcagtttcat
      901 ctgggacttc aaaattgata gtcacagaag tccagacagg atttccttta gatagtgctt
      961 ttttgatatc atcgagttga gcatcggttt ttggaaaagt gtgataatcc gtttggacga
     1021 tttgctgagc aagggaggca actgggggaa catagacgac catagcccag tctccgccac
     1081 tgatatcacc aacaaatcct tcattaggat cgccatgagt cccatctgca ttgaaaacag
     1141 ggacgtaatc caattgctca gctaactggt ttttagttgt ttcataacca taaaaagata
     1201 aaagcataga aagcgaagtg acttcacagc cattttctaa agaaggagaa tcaaactggc
     1261 tctctaatgg aacatctagt aaaacttctg ctttctcttt tgtctttttc gttttttgtg
     1321 ttgaactttt ttgtgcagtg ttcattgtat gttctggaga atggatatgt aggtaataac
     1381 ttgtaccgct taataaaaga gcagctgtgc cccctagaaa caatagtttt ttgaacgttt
     1441 ttttcttcat ttgaaatctc ccttttcagc tgtttatctt tcctattata aatgtatttt
     1501 tattggaaa
//
LOCUS       DACCXM010000239         1491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCXM010000239 DACCXM010000000
VERSION     DACCXM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.249"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            84..>1491
                     /locus_tag="IVY61_002857"
     CDS             84..>1491
                     /locus_tag="IVY61_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HAP7971904.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAV"
BASE COUNT          492 a          217 c          255 g          527 t
ORIGIN      
        1 taaacttttt cctacagcaa tttaataaac gtctttaata tttaagctta ataaaaagat
       61 aaaacaagtc aggagttgca aaaatggaaa gaactgctcg gctcaaaaaa aacactattt
      121 gggctctggt gtttcaaata acttctattg tctgtggtat gatcttgcta cgtttgatat
      181 taacaaagta cggatccgag gttaatggaa tgattagctc tattaaccaa tttctacaaa
      241 ttatttcttt tttacaatta ggagtaggcg ctgtaatgca atcaactcta tacaaaccac
      301 tagcagaaaa ggataattta atgataagtc aaatcttagc ttctggaggg aagttttatc
      361 ggcaaattgc atatattcta ctagtatacg tgggtttgtt acttgcagtc tattatgttc
      421 tcgttcctaa tgaatttggg agatcatata caatggcaat gattgcaaca ttaagtatta
      481 attcatttgc tcaatattat tttggtattg ttaatacttt gttattaagt gctgaccaga
      541 aaatttatat tacgtatatt gcacagacaa tagcatttat ttctaataca gttgcatgtg
      601 ctttattaat tagtctgaac acagagatcc ttatggtaaa acttattact gctataattt
      661 atcttgcttg tccaatatat tatagatggt atgtggatcg aaactataat ataaagagga
      721 atgtgtcata tgataaggaa ccgatccctc aaaaatggaa tggggttgca caacacgtag
      781 cttctgttgt actagatagt actgatgttg tagtgttatc tatattttct aacctgatat
      841 atgtatctat atataatgtg tacaatttag taatatacgg agtaaaacag ctattcatgg
      901 ctatgacaaa tggcgttcag gccttactag gggaactttg ggtcagaaaa gaaatagaag
      961 ttctttacaa aacatttgca tggttcgaat ggattgtaca tactttgaca gtttttattt
     1021 ttggctgtac cggtatacta atcgttccat ttattcaagt atatacaaga ggagtaacag
     1081 atgctaatta taatcaatta tcattcgcag ctttacttgt aactgctaac gcacttcact
     1141 gtctgcgtac accttatcat gtaatgataa aagcagctgg taaatataaa gaaactcaaa
     1201 atagttttat aattgcttct ttgttaaatt taggtttatc attaattttg gttaaaccac
     1261 tagggctagt tggagtagca ttaggatctc taatttcaat cacatatcat acactttgga
     1321 gtgcaaatta taattcaaaa tatttgctta aatggccgat gaaaaaattc tataaacagt
     1381 tatttgtaaa catcttaact gtagttcttt cttttggggc gacatcacaa ttgaaaatga
     1441 tgtcaatcac atatttggat tggattgttc tcgcaattaa atgtgcagtt a
//
LOCUS       DACCXM010000240         1480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCXM010000240 DACCXM010000000
VERSION     DACCXM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.250"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            64..972
                     /locus_tag="IVY61_002858"
     CDS             64..972
                     /locus_tag="IVY61_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP7971905.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="IVY61_002859"
     CDS             972..>1480
                     /locus_tag="IVY61_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971906.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DACCXM010000241         1459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCXM010000241 DACCXM010000000
VERSION     DACCXM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.251"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            131..505
                     /locus_tag="IVY61_002860"
     CDS             131..505
                     /locus_tag="IVY61_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7971907.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVY61_002861"
     CDS             complement(619..1248)
                     /locus_tag="IVY61_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7971908.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVY61_002862"
     tRNA            complement(1382..1454)
                     /locus_tag="IVY61_002862"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          489 a          223 c          258 g          489 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattat
//
LOCUS       DACCXM010000242         1453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCXM010000242 DACCXM010000000
VERSION     DACCXM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.252"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            192..806
                     /locus_tag="IVY61_002863"
     CDS             192..806
                     /locus_tag="IVY61_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7971909.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(830..1180)
                     /locus_tag="IVY61_002864"
     CDS             complement(830..1180)
                     /locus_tag="IVY61_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7971910.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1174..1437)
                     /locus_tag="IVY61_002865"
     CDS             complement(1174..1437)
                     /locus_tag="IVY61_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP7971911.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          461 a          273 c          233 g          486 t
ORIGIN      
        1 cttgattgac atttatttac aaaaaatatt gccatttaaa aagaaaagtg acacatacta
       61 tttcctccat cattagtttt tgtctatata aggtataccc taactagaca cgaccgcgac
      121 attgaaaaat ctggttttct cgtgtatgat tagggtgttt tttaaatagt tgcagaaagt
      181 aggtaaaaat tttgagagtt gcatacatac gtgtctcatc catcgatcaa aatgaacaac
      241 ggcagattga agaaatgaaa aaattcggtg cagagagaat tttcattgag aaacaatctg
      301 gcgcaacgat tactcatcga cctgtttttc aagaagcact ggatttcgtc cgagatcaag
      361 atatttttat tgtggaagct attgatcgac ttggaagaaa ttacgacgag atcatcgctt
      421 cagtcaatta cttgaagaag aaaaatgtgc agctgatcat cacaagtcta ccgatcatgg
      481 ccgaagcaat tggcaatcct ctcctggata aattcattaa agatttgatc attcaaattt
      541 tggccatgat cgctgaacaa gaacgtaccg aatctaaacg acgtcaggca caaggcatta
      601 agattgcaaa agcgaacggt gtttataaag gacgtcctaa gctatacagt gccaatgcaa
      661 aagatcccca acgtcgtctt gtttataaaa acatcgttga ggatctaaaa aaaggtgttg
      721 cgatcgctaa gattgcaaaa gactacaatg ttacgagaca aaccgtatat agaataaaaa
      781 aagacagcat ggtcaatcac gaatagttga tcatgctgct gtttctattt tatttgctta
      841 ctttttgaaa aactgttcta actttcatca ataactcctc aactaaacta tcatgcgctt
      901 tttccaaaaa aatacactct ctggcttcat aatcaattgt cgttagttga tctaataata
      961 cttttcctgt tgtcttcatt tcatattctg agtttatatt tacatatagc ggatagtttc
     1021 tagttgtact agaaattgga gcaactgtaa caacgttact ataatccgca ataatatcat
     1081 gacttaaaac aatataaggt cttttacctg tctgttcgtg tccagatcga ggtgctgtat
     1141 tcaataatag aatatcgcct tgtctaggtt ttctcaccat ttttcattac ctactgcttc
     1201 acctaaattt tgaatcgtca catgatttct ttcatcagtg taattttcaa ataactgttc
     1261 aattgttaat tcattttcta tattagccgc ttcgataatt agccgcttct gaccgtttac
     1321 ttcttcttgt attatcttca ctttgtcttc tgtatgaatt ccaagatatt ccataatata
     1381 ctttggcaat ctaaggccat tactatttcc ccattttttt ataggtaaca tttccatagt
     1441 agacacctct ttc
//
LOCUS       DACCXM010000243         1431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCXM010000243 DACCXM010000000
VERSION     DACCXM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.253"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            51..1163
                     /locus_tag="IVY61_002866"
     CDS             51..1163
                     /locus_tag="IVY61_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7971912.1"
                     /translation="MLFSLSAFSTGTILGWQFYNNEHSQIVAHAQSAQKKLILTTNEQ
                     QLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGSVQKA
                     LTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMTD
                     EEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQHT
                     KFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQV
                     LSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDAI
                     IMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          507 a          263 c          233 g          428 t
ORIGIN      
        1 aaggaaaaca tgctaaaaaa agaaaatatt catttttaat gatcgttgtc gtgttgttta
       61 gccttagtgc gttctctaca ggaactattc ttggatggca attttacaat aatgaacact
      121 ctcagatagt tgcccatgct caatcagctc aaaaaaagtt gatactgact accaatgaac
      181 aacagctcgt tgaaaaaatc gatcaactat taagaaaaaa tgattatatt ggctcaattt
      241 acgtaaagaa aaataatcgc atcatcatgg aaaaaggcta tggctatgcg aataaactca
      301 cgcaacaacc aaatgatcct tctctttatt atcagattgg ttcggttcaa aaagcattga
      361 ctactctttt gattttaaaa caagtcgagc gtgggcacct ttctttagat actaagctgg
      421 ctcaattcta tccacaaatt gctggcagcc agcaaattac tatccgtgac ttgctgtata
      481 tgcgctcagg cttgcaccga actgcttctc caaccatacc gatgactgat gaagaagtcg
      541 ttcaatttgc gattcagcac ttaaaattta cagattatca gacctctcat tatgattcat
      601 tgaactttac catattgact ggaattttga tcaaattaac aaatcaaccg tatgaaaaat
      661 tattgaccga tgatatcatc gaacctttag gattacagca tacaaaattt tatgaccaag
      721 tcaaaaattc atccaatcat gcactatcct atcaaatgtc taaagaggat gattacttta
      781 aaccactgac agaatctgaa acagagatcc ataacgaatt agggactggt aatatcagta
      841 tgtccgtcta tgatttaaat acgctgttta cacaagtttt atctggaaaa ctagtttcca
      901 aagacttact cttctcatta tggcgtgaaa atgcgaatgg acatccatat agtggtgggg
      961 tatatagcgg agataattat atcctgtcgc aaggaaatat cgactctttt gatacatctg
     1021 ctatatttag caaagattca caggatgcta tcatcatgga aagcaatata aaagctgaca
     1081 aaactatcaa actgcctact gctgatctaa gaaaccaaat ctatgacctg atggaaggaa
     1141 ctactaagtt ggcaaatagc tgatatgatg aaaagatagc aaatactact tctttgaata
     1201 gcatttgctt aaaaaaagta ctcgactaaa cgtaggaaag agtgtgggac aaatctaaaa
     1261 atcaattttt gctacagact ttaattccaa ataaacagaa cagaaagagg tgttcttctt
     1321 tctgttctgt ccaaattatt ctaagatttc tctttaccaa cattatttga caaagggcct
     1381 attaaatcta aaatagtatc tttagattgc taaaatatgt gctagcaaat c
//
LOCUS       DACCXM010000244         1384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCXM010000244 DACCXM010000000
VERSION     DACCXM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.254"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            72..145
                     /locus_tag="IVY61_002867"
     tRNA            72..145
                     /locus_tag="IVY61_002867"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:106..108,aa:Ile,seq:cat)
     gene            691..1257
                     /locus_tag="IVY61_002868"
                     /pseudo
     CDS             691..1257
                     /locus_tag="IVY61_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT          464 a          236 c          252 g          432 t
ORIGIN      
        1 caaagaatga aacccattat tatctagtct atgtccattc tttttgtttg cagtagttgt
       61 gatggctttc cgtactctta gctcagttgg ttagagcaga cggctcataa ccgtccggtc
      121 gtaggctcga gtcctacagg gtacatactc taagtgattt aaattgcgaa taaaatataa
      181 aaatgactcc ccttttgact cccccaagca gtatatggat gtctctaaaa gttgtttttt
      241 tagataataa aaagctgata ataagcgaaa aacacttaaa atcagcttta aaccctattc
      301 tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta ccttccttat
      361 atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa cggccaaaca
      421 aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg caagcttttg
      481 aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc ccttattgtt
      541 gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta taaatgctag
      601 taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa gataaaagtg
      661 taaaaacgat ttgttttagg aggggaaacg atgtgtaccg caatcactta tgcaacgaaa
      721 gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt ggtcactatt
      781 actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa acattatgca
      841 atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc tacaaatgaa
      901 aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa agaattacaa
      961 gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg gcaatgctca
     1021 accatagacg aagctaaaga attattggct actatcaatc tagtcaatat cgattttagt
     1081 gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa atcgatagta
     1141 attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt gttgacaaat
     1201 aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct atcaaatgga
     1261 acgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg aagtcccttc
     1321 tgtagaataa agttaacgac aaccaattca cagaaaagag gacttcccta tgaatgattt
     1381 tact
//
LOCUS       DACCXM010000245         1382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCXM010000245 DACCXM010000000
VERSION     DACCXM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.255"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..710
                     /locus_tag="IVY61_002869"
     CDS             <1..710
                     /locus_tag="IVY61_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7971913.1"
                     /translation="SDIKKNTKVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEH
                     PTSVKYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDL
                     ENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAK
                     IIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDS
                     VELREKVLRSIIDKSIE"
     gene            940..1170
                     /locus_tag="IVY61_002870"
     CDS             940..1170
                     /locus_tag="IVY61_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP7971914.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          477 a          202 c          220 g          483 t
ORIGIN      
        1 tgagtgatat taagaaaaac acaaaagttg gcgatgctac tattattcgc ttttgtcaaa
       61 aaattggttt ttctggtttt tctgatttaa aaattgaaat tgcaaaggaa gactttagtc
      121 aaaaaaaaga acatccgact agtgtaaagt actatgatga aattgctaat agtttaacag
      181 aagctctcta ttccactatt aggcttttag acgaagaaaa attgaatgaa gccattaaaa
      241 tgatcactca atcaaaaaac atatatatat ttggagtggg atctagtgga aatactagtc
      301 ttgatttaga aaatatgttt cttcgcgtag gagttcaagc aaaagctgta ctagaccctc
      361 attttcaagc ccaagtcgct tctttattaa cagttaatga tttagtaatc attttttctt
      421 tatctgggaa aacgaaagat acttatgatt cattgaaaat agctaaaaaa aatggcgcaa
      481 aaattattgc tataactaac tatattcatt cacctatagg aaagtctgca gacttagttt
      541 tacaaacagc cattgaagaa tttcttaatg gtggatcttt agctggaaaa atatcacaac
      601 tttatatttg tgatctatta gttcatggtt atgaacaaaa caataatatt gattcagttg
      661 aattaagaga aaaagttttg cgctctatta ttgataaaag tattgagtga actaccccca
      721 cttagctaaa cctaacggtt tttaacgatt gaagtaggca attcttggga tgagagcata
      781 cttgatagct tattttttac taatagcggt ggctcttatt gaccaaatat ccctgtagtt
      841 cctacggttc ttgattttat ctaaagatat ttttatcgtg gaagctattg atcaacttga
      901 aagaaaaacg tgtagctcat tatcactagt ttatcgagta tggatgaagc aatggatcat
      961 cctctcttaa ataaatttat taaggatctg atccttcaaa ttttttccat gatcgatgag
     1021 caggaaccta ctgaatctaa acgacatcgg gcacaaggaa ttattaagat tgctaagcga
     1081 atgttttcta taaagggtgt tctaagctat acagtgttaa ttcaaaagat tcccaatcat
     1141 cttgtttata agaacaacgt tgaagattaa aaaagtgttt caatgctaaa attgcaaaat
     1201 acttcaatgt tatgagacaa actgtctata agaatcaatt tccacccatt tcatcacttg
     1261 ttaaaagttt atgatccttt tgatttttat attaatttat ctgtatttaa tacagatttt
     1321 atttattagt tcatctgcct attgtatttt attttgttgc cttaccatct gaagtaaata
     1381 ta
//
LOCUS       DACCXM010000246         1366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCXM010000246 DACCXM010000000
VERSION     DACCXM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.256"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            80..>1366
                     /locus_tag="IVY61_002871"
     CDS             80..>1366
                     /locus_tag="IVY61_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP7971915.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          466 a          263 c          257 g          380 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccctttttt catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc
     1141 cttattggca gaacttcccg aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct
     1201 tctgacctat gaagaaggca tcaccaacgc aatgatctat ccttattcca atggaaaaat
     1261 agaagcgaag aatacccaca taaagacaat gaaacgagta tcctacggat ttaaatcatt
     1321 tgagaacatg agaattagaa tctttttgat caatcaatta atcaat
//
LOCUS       DACCXM010000247         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCXM010000247 DACCXM010000000
VERSION     DACCXM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.257"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>1355
                     /locus_tag="IVY61_002872"
     rRNA            <1..>1355
                     /locus_tag="IVY61_002872"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACCXM010000248         1343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCXM010000248 DACCXM010000000
VERSION     DACCXM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.258"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(310..924)
                     /locus_tag="IVY61_002873"
     CDS             complement(310..924)
                     /locus_tag="IVY61_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7971916.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          437 a          266 c          222 g          418 t
ORIGIN      
        1 ttcatcaacg tgtattaaga gacaagaaag aatccatatg gattctttct tgtctctttt
       61 tccactactt tttatttttc tgacactaca tattcttata actcatttct cataactgtg
      121 aaacaactta taaaaataac aaactttaaa caaaacaagc ttaaaatcaa agatgatcca
      181 tcttaaaata actaaaacaa aaaaagtagt cgttgttaat cctcattaat aatcgttttc
      241 tttttttcaa acgctccatt tttatgtgga acattatttt tttaaggaac tttgtcaaca
      301 tgtggataac tattttccac ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac
      361 ttgctgcagc aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg gatctagatt
      421 tggcaaagtc aagactgcat catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa
      481 ttcaaataat cgcaaaccac tgctatgttg cgatttgata cttcgtgaca aatcaaatcc
      541 agtaacaatg attaatggat gaccattttc tttgatccac tgagctagtt cgatgattgc
      601 cgggtttctt ccttcattcg aaagaagaag aaaaatgtct tctgatttga cagaaagctg
      661 atcaataatt gcctgtcctg caccttctag cgtttcatag acacctccgg ctggattttc
      721 aatcaatata cttggtacaa gatccggggt ttgatcccaa aattcaaatg ctacaccact
      781 tagtgtgcgg ctagcatata tctgcaatct accaccttga ttaatttgtt tcgcgatcca
      841 agtgccagct tgaagcatca gcggctcttc tatccttaat ttctcttcca attggtgata
      901 atagtcaaaa taacataaac tcatttatct ctcccgccta gctatacttg acatgaatag
      961 aaacagctgc ctaatctaag gaagtacata aaaaaacagt agttagacag taacacatgt
     1021 ggtctttgct gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat accttgacat
     1081 agcaaataaa acaacgtgca gaccatggaa aatatctaca taggggtaga attttcaccc
     1141 tagattaggt acaataaatt aggtcgatgc actgcattga ctatggcaaa atgatcggtc
     1201 agggtgtatg tgtgtgactg gtcattccct gattcttagc gccaactaag ggtcagggag
     1261 ccttttttta ttcagctgtt tctatctgaa tttattgtaa gtaatctctt atccaagtgc
     1321 aagcaagaaa atacaaaaaa aca
//
LOCUS       DACCXM010000249         1324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCXM010000249 DACCXM010000000
VERSION     DACCXM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.259"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..121)
                     /locus_tag="IVY61_002874"
     CDS             complement(<1..121)
                     /locus_tag="IVY61_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HAP7971917.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYN"
     gene            178..1305
                     /locus_tag="IVY61_002875"
     CDS             178..1305
                     /locus_tag="IVY61_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7971918.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          459 a          229 c          259 g          377 t
ORIGIN      
        1 cattgtaaat tgtttggcgt ccacgtttaa gctgcttcgc aacgatagca accggagttt
       61 cttgatgaaa atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca
      121 tttatgttca ctctccttgt atgctttagc gggtattaca attggagtgt aacataaatg
      181 gcttttttat ttgtctcgct taattataca aacggcgaag atatcaatga tgatggctca
      241 agaaaaaaag tggagaaagt tggcggagac acccgaccag aggccgaagc tgctttacga
      301 aaagttttat cagatattga cgaaacagga caatactttt taggtacgga tactcgagta
      361 aaacaatacc ttgatttttg gatggaggaa tacgttaaac taaatctaaa atacaatacc
      421 tatgaaaact accgatttac catcaaaaat catataaacg gttatttagg aaagaaaaaa
      481 cttacggatc tctcccctgc tcttttacaa aatttcatca atgctgaatt taaaaagggt
      541 tactcgaaga aaacaatgac tattactcac tctgtcctta agaatgcgct gaatatggcg
      601 gtttatcctt ggggattaat caagcaaaat cctatgctgt atgtaaaaat accaaaatat
      661 gaagcacgac caacgactaa aaaagatcta aaaatcattt ctcttgagga ctttgatcat
      721 atgctagaaa ttactcctga aggacatcct ttctatattc ctttgaatat tggattttat
      781 acgggaatgc gcgttggcga agtttgtggt ctgacgtggg atgatgtcga tttttcaaat
      841 ggaacaatta ctgtagagaa acaaatggta aagaatgacg gcgcatgggt atatggtacg
      901 ccaaagacaa gcagttccaa tcgaacgatt tttattggac aaaccttgct agcaattctg
      961 aaaaaacata agaaacaaca attagaaaat cgaatgaagt atggaaagct ctacattgat
     1021 tcaaatgcag tatgtacgaa ggaagacggt gagctagtta cgccaagtgt agtgaaatgg
     1081 aatactagga ggatatcgaa tgcactctcc ctctctttta acttccattc tctcagacat
     1141 actcatgcta cacttcttct cgaaaatggc gcaaaaatga aagaaatctc tgaacgattg
     1201 ggccacagca gaatttcaat tacgatggat acttactcgc atgtaacaga taagatgaga
     1261 aatgaaacgg tcgatatcat ggaaaatctt agaaagaatt cttgattttt gccaccgaaa
     1321 aaaa
//
LOCUS       DACCXM010000250         1299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCXM010000250 DACCXM010000000
VERSION     DACCXM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.260"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            39..1271
                     /locus_tag="IVY61_002876"
     CDS             39..1271
                     /locus_tag="IVY61_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7971919.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          423 a          248 c          295 g          333 t
ORIGIN      
        1 atagttacca gaaaaaatgg tgaggaggaa tttttattat gttatctgat tttaatgaac
       61 ttctaaaaaa atatgcacgc ttaattgcag aaactggcgt agccacagaa aaaggacaca
      121 cagttgtttt acagatcagc gtggaacagg caccccttgc tcgattgatc acacaagaag
      181 cttataaact aggagccgct gaggttatcg ttcaatggac tgacgaccag atccaaagag
      241 aatttctttt acatgctgca acggatcgta ttgagaatgt accacaatct aaaatcgatc
      301 aagcggatga atggcttgaa aaaggagcaa gcaggatcag cgttgtttct gccgatcccg
      361 atgcatttgc tggtgtagat agccatcgag tagcgtctta tcaatcagcg aatggcaaag
      421 cactaatgaa tttacgcaag gcaactcaag caaataaagt cagttggact gtcgtggctg
      481 cggctggtaa acaatgggca gcaaaagtct tcccagaact gccagaagaa aaacaagttg
      541 aggcattatg ggatcagatt ttcaaaacga ctcgtgtcta tgaagaagac cctgtcttag
      601 catggaagaa gcatgatgaa aaattagcaa aaaaagcaga agaattgaat cgagaacaat
      661 tttctgccct tcactatact gctcccggta cagatatcat tatcggattg cctaaaaacc
      721 atctttggga aggcgctgga agctataatg ctcgtggaga aaaattcatg gcaaatatgc
      781 cgacggaaga agtgttcaca gcacctgaca gtcatcgagt agatggttac atttcaagta
      841 caaagccact gagttatgct ggcaccatca tctcgggtat gaaatttact tttaaagatg
      901 gaaaagttgt tgatttttct gccgaacaag gagaagatgt cttagcaaaa ctgcttgata
      961 cagatgaagg tgcgcgtcgt ttaggagaag tcgcacttgt acctgaccct tcacctattt
     1021 ctcaatctgg catcatcttt ttcaatacat tatttgatga aaacgcatcg aatcacttag
     1081 cactaggttc tgcttatgcc ttcagtgtga aaggcggtac agaaatgtcc gatgaagaat
     1141 tagcagaagc tggattgaat agaagtcaaa cccacgtaga tttcatggta ggatctgaca
     1201 agatggatat cgacggaatc cgtgaagatg gctctacagt accgattttc cgaaatggcg
     1261 attgggcttg agtatacttt cgcacacatg caaaaaata
//
LOCUS       DACCXM010000251         1269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCXM010000251 DACCXM010000000
VERSION     DACCXM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.261"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..596)
                     /locus_tag="IVY61_002877"
     CDS             complement(<1..596)
                     /locus_tag="IVY61_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7971920.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNL"
     gene            complement(596..>1269)
                     /locus_tag="IVY61_002878"
     CDS             complement(596..>1269)
                     /locus_tag="IVY61_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7971921.1"
                     /translation="IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQ
                     YLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDID
                     YSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETS
                     SMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLIN
                     DIEVN"
BASE COUNT          432 a          217 c          142 g          478 t
ORIGIN      
        1 tcaagattcg aatatttacc aaactgtttt tttactgatt tagggaaacc aaataggttt
       61 ataatataat ccaattcatg tatacagtct aattctactc ctccccccat agatttaaag
      121 gcagaataat tatttctata atcagtacct tttctccaat caggaagata agtagaacaa
      181 atagctctta cagaatatac ttttttactt tgaatactat tacgaacaac ttccatgata
      241 tttttatacc tcagtggagc agcaacataa atatttttgt aatcataatt atattgagta
      301 agatcaatac tctcagaaaa agcaggtttt tctaaaaata tgtaatcaac tttttcaaca
      361 attacattta gtgtttccag atgcttaaaa gtcggatttg taataaaagc tatatcataa
      421 tgtgtatcca aatcgtctct attttgaata tacgttattt cttcaaactc tttttttcca
      481 ctgctactat aagctgttat atcaaaatta atagaattct tttttccata gcttattaga
      541 ttttttagat gtctctttcc aattgaccct aacccaaaaa aaattacttt catcattaat
      601 ttacctcaat gtcgttaatt aagttaataa tatacttatc tttttcaaaa gtatattggg
      661 gttccatttt cttttccaaa agcgtgaaaa attctttaat ctcctctaaa tatgcatctt
      721 caataatagt atgtgaataa ttattatcat gcataacctc atcgtacaat tgaatatctg
      781 tcatactaga agtttcttca ttccatgaaa atagagaatc cgggttacct tcccattcta
      841 ttaaaatctt ttcaccaatt atagttaaat ttcttttagc tactcttgaa acaacatcta
      901 cacttaaagt tccaactata ccagtttcat gctcaataat aagagtgaaa ctgtccgaat
      961 aatcaatatc aagattggat aatttctggg tcattacagt aaacgattta actttcccaa
     1021 atgtatctat aatccaaggt aattcaatag cgaatatttc tctacaccca ttagtttttt
     1081 tgtttgaaac aaaaaaattg cgataatctt cccaaggatg ccagtcgggt aaatactgtc
     1141 ctacatggta ttgataagaa atttttttat tatgcgtcac tttagactta atatattgaa
     1201 tttcttttct cttcaaaaat gtagaagata gataaagtaa tctatctttc tttttagcta
     1261 cttcgatta
//
LOCUS       DACCXM010000252         1265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.262, whole genome shotgun sequence.
ACCESSION   DACCXM010000252 DACCXM010000000
VERSION     DACCXM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.262"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            316..570
                     /locus_tag="IVY61_002879"
     CDS             316..570
                     /locus_tag="IVY61_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7971922.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IVY61_002880"
     CDS             571..888
                     /locus_tag="IVY61_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7971923.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          440 a          205 c          251 g          369 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaa
//
LOCUS       DACCXM010000253         1261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.263, whole genome shotgun sequence.
ACCESSION   DACCXM010000253 DACCXM010000000
VERSION     DACCXM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.263"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..299)
                     /locus_tag="IVY61_002881"
     CDS             complement(<1..299)
                     /locus_tag="IVY61_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971924.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(305..535)
                     /locus_tag="IVY61_002882"
     CDS             complement(305..535)
                     /locus_tag="IVY61_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971925.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(785..985)
                     /locus_tag="IVY61_002883"
     CDS             complement(785..985)
                     /locus_tag="IVY61_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7971926.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          440 a          206 c          189 g          426 t
ORIGIN      
        1 taatctttgt atctttctgg atcaacgaaa gtatacttta tatagtcata acgccgatga
       61 tcgcttcgag cgtctagcac atcagtcacg atatcaaaca aaaaatatac gtctttcttc
      121 attctagttt tcgcagcagg gattttaaag tagttcttat tagaatagta gagattaatt
      181 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
      241 ttgacaaatc tattcgaagg atcaaattta aaccataatt gcgtctcatt aaaaagcata
      301 accattactc cgatctattt ttaaacttag tctctacctc taatatatat cgagttttta
      361 ttttgccttc agagaatact gtttcttttt ttgcagttac aggttgttta ttttcggaaa
      421 aagctaatat agctaaaatt gaaacatcca tctggaattt gtctttttcg ttgctttgct
      481 cataaaaatc tgcatattca tcactaatat tttttctaat aaattcttcc atcataaaga
      541 tcacctcgaa aagagtatac gaacaaacgt tctttttgta aaggtggaat ttattatact
      601 aaataaatag gtgaacaagt acttgtgcca aattatgtgc caaaaaaaat cgaatttaat
      661 aaaacacaaa caaaaagaaa tcctattatg ataacatttc ttataataac aaacacaaca
      721 gaagacgtgt aatagttagt caggaacgta caaataaccc ctgtatcctt tgcggtacag
      781 ggggttattt tgtataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg
      841 atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc
      901 catcaataaa cagtcgaaga gaaaataggg aaaaattgat tactaaagaa aaacttacta
      961 ggtcgacggt gtagttgatt ttcatacttc aatagattcg aattaaacga tttcgattgg
     1021 cttataccag tcctatccaa cgaaagatat atcgttctta actctagcgg tataacttga
     1081 agctttacgt aaagccaatg ggaatgaact ggagatgact ttttattatt tttagtaata
     1141 gctgatgggt taatacttac aactttcctt ttcaaagtac catataaaaa aacggtgggg
     1201 ttgtttagcg aaataaagga tctaagccaa tagcttagat cctttattct atgctttata
     1261 t
//
LOCUS       DACCXM010000254         1259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.264, whole genome shotgun sequence.
ACCESSION   DACCXM010000254 DACCXM010000000
VERSION     DACCXM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.264"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            66..1235
                     /locus_tag="IVY61_002884"
     CDS             66..1235
                     /locus_tag="IVY61_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7971927.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          455 a          190 c          206 g          408 t
ORIGIN      
        1 aaaaagaaag tccattctgt aaaattaaag ttaccacaac cgaaatttta taggaggact
       61 ttctcatgcc caattttact acagaaatta tggaaacact aatcaataaa ggtgatttag
      121 atgaattatt tcgatgtcat ttagaattag ctgttaattc attattgcag gctgaattaa
      181 cagcttttct tgattatgaa aaatatgatc gtgctgggtt caattcaggt aattcccgta
      241 atgggaatta ttcacgttca ttcagaacag agtatggaga attaaatttg gtaattccta
      301 gagatagaaa tggtaagttt tctcaacaaa ctttacctgc ttataaaaga accaatgact
      361 ctttagaaac cactattatt cagctatttc aaaaaggaat tactatgtct gaaatctctg
      421 aattgattga aaaaatgtac gggcatcact atacgccaca aaccatttca aatatgacta
      481 aaatagtttc tgaagatatt attgccttta aagaaaggtc cttagaatcc agatactctg
      541 tcatttttat ggatgctact cacattcctt taaaaagaca aactgtttca aaagaagcag
      601 tttatattgt aattggcatt cgattagatg gaaccaaaga ggttcttggg tttagtcttg
      661 ctccaactga atctgcttat gtttggaaag agattcttca agacctaaaa gaccgtggtt
      721 taaaagaggt tttattagtc gtaactgatg gtttaagtgg cattaacgat agtattcata
      781 gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc
      841 ataaggttcg tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gtttatcaag
      901 caagttcaaa agaagaagca aataatcaaa taaggtttat gatagataaa tggaaaaagc
      961 agtatccacg agtggttaaa ttgctcatga atcctgctat attaaccttt tataatttcc
     1021 ctccgtcaat cagaagaact atttattcaa ctaatttgat tgaaggtttt aacaaacaat
     1081 taaagaaata taccaaaaga aaagagcaat ttcctaatga agaatctctg gagagatttc
     1141 tagtatctca attcaacaac tataatcaaa agtttctatg ccgagttcat aaaggtttta
     1201 aagaaataca tgatacatta gaatcaatgt tttaacttga tcaacagaat gggcttttc
//
LOCUS       DACCXM010000255         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.265, whole genome shotgun sequence.
ACCESSION   DACCXM010000255 DACCXM010000000
VERSION     DACCXM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.265"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            200..742
                     /locus_tag="IVY61_002885"
     CDS             200..742
                     /locus_tag="IVY61_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971928.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IVY61_002886"
     CDS             832..>1246
                     /locus_tag="IVY61_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP7971929.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACCXM010000256         1245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.266, whole genome shotgun sequence.
ACCESSION   DACCXM010000256 DACCXM010000000
VERSION     DACCXM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.266"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            26..898
                     /locus_tag="IVY61_002887"
     CDS             26..898
                     /locus_tag="IVY61_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7971930.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          374 a          229 c          301 g          341 t
ORIGIN      
        1 ttctaaaata aagggagaaa tgtctatgct atacggtgca attgaagcag gcggcacaaa
       61 atttgtgtgt gccattggcg atgaagaaat gacgatcaag gaaagagtga gctttccgac
      121 aacgacacct gaggaaacaa tgcctttagt cattgacttc ttcaaacaat accaagcaga
      181 tttagcaggg attgggattg gctcatttgg gcctatcgat atccatcgcg attccgccac
      241 ttatgggtac attacctcga ccccgaaatt ggcatggcaa aattttgatt tcatcggtac
      301 catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg cggctgccta
      361 tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact atacgatcgg
      421 tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt ttagtcatcc
      481 agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg gcagctgtcc
      541 atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa accgcttagg
      601 gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag cctcctatat
      661 tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca tttttggcgg
      721 tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg caaaattggt
      781 caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag cattagaaga
      841 caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac attcataaac
      901 gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa aatttagtca
      961 taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaagatc aagccttgaa
     1021 tgagataaat tcttgctcga aagttatgga aatttcgata gccgtaggcc acttgtattg
     1081 tgcaatagtt ttttacaaaa aaaagggggt gcggatattt ttttggacct ttttaacaaa
     1141 taggttctgt tgcaaagttt taaatctact atcagataag gtagaataat agaaaaagat
     1201 agcaggagga atgacgatga gtcattttaa aggaaagcaa tttca
//
LOCUS       DACCXM010000257         1243 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.267, whole genome shotgun sequence.
ACCESSION   DACCXM010000257 DACCXM010000000
VERSION     DACCXM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.267"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            825..1037
                     /locus_tag="IVY61_002888"
     CDS             825..1037
                     /locus_tag="IVY61_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971931.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          462 a          187 c          206 g          388 t
ORIGIN      
        1 aatattatca tcgaaacagc taacaaaatt gtcataaaaa acgagatggt tgatattaga
       61 acttgtgttt ttgtttaggt ctagagataa agagagacaa tttccaaata tatgtaatga
      121 atacaaaaag acgtttgtcg cggattaccg ttacaaacgt cttttattga ttttccgaga
      181 tctcatcagt cgccatttgg aatggttcac tccttatcta gattcgttta atgctcggac
      241 tgcttgttcg attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat
      301 aaaacggtta gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt
      361 cataaaattc atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg
      421 aacttatacc aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt
      481 tccttattta ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca
      541 atcatcaagg ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa
      601 taacttagca atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt
      661 tgtcacaacg tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatactcaa
      721 aataaaaaat aaaaaaatgg ataaaaacgt tttcaaatac aaggtaattt gttattataa
      781 aataaagaaa aaacaaaata aaaataaaga aaagagggaa tgaaatggta aaagcagaag
      841 taggaagatc ttatatatgc aaaccgatcg gagtatctca tgaactaatc ggctgtattg
      901 aacgtacata tatcaacacg gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc
      961 aacttatcaa tgaaagcaag tatcgcatgc tcgtaaaatt ctgtgatatt agcgcacctg
     1021 ctgaattggt ttcttaaaca gtatttttga gaattcgcaa gaactccctt ataatataaa
     1081 taaagacaga agggagcgag aagatatgcc aaaaagaaaa agttcaggga aaatgggaat
     1141 tattcttgca gtgatctctg caattcctgc tattttagct attgttcgtg cgatgaagga
     1201 atcaagagaa agttaagcac acattatgag cgtcaaacaa aaa
//
LOCUS       DACCXM010000258         1216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.268, whole genome shotgun sequence.
ACCESSION   DACCXM010000258 DACCXM010000000
VERSION     DACCXM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.268"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            18..971
                     /locus_tag="IVY61_002889"
     CDS             18..971
                     /locus_tag="IVY61_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7971932.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            943..>1216
                     /locus_tag="IVY61_002890"
     CDS             943..>1216
                     /locus_tag="IVY61_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP7971933.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
BASE COUNT          437 a          189 c          234 g          356 t
ORIGIN      
        1 ggaaattgga gggaactatg gaaaacttac ggactgcatt taaaacatca gataaactag
       61 gaaaagttgt aatgcttaca ggtagaaaag gcggcattgg taagactaca gacaatgatt
      121 tattagcgat tgtttctagt caactttttg aaaaagacgt gttgctaatt gattatgacc
      181 aacaaagaaa tactacttcc aacattggat caacgtatca aattactagc tttgatcgat
      241 ccatgtctgc agcaatcaaa aaaggggact gggtttcagg tattactcaa gtaagtccac
      301 atttatatat tatggctggc tctcctggat ctgaagaact taatgaatac ttgtccgaaa
      361 aatatcctga tcgtcgtaag agatcattgg ccttcattaa acctttagaa gaattgagaa
      421 aaaatttcga ttatatcttt atcgactgcc caccatcaac agataatgtg gttcgtgcat
      481 tcttaactgc tgcagattat attattgcaa tgcaagaatt aaaacggtac gcaatggaag
      541 gaacagaaga ttttattaat aaagttttag ttccgattgt cacgaatttt gaagagtctc
      601 atcttcaaat cattggaatt ttgcctgttc ttttctcagt gcgacgttcc tctcaacacg
      661 caaactatca aaaaacaatt gataagtatg gtgaatcaaa catttttaaa agcattgtta
      721 aaggctcgga tcggttagaa atgtatggtg agaatggtat ccaattaaat gattatgtag
      781 atcgaagatg gtgggctatt tttgcagaca tctttacaga attagaagaa agaattgaat
      841 actatgaaca acatggtgat attgaaggat ttgattatac acctaaatat gctgactcaa
      901 tggccaatca tatcctacct cttggaaagg agattaaact aaatggcatt attaccaacg
      961 aacaaagata agaagaaaat tggtgctgaa gatatttcaa ttaaagatga tattaatgtc
     1021 gagttagggg aacgaaagta cactgcgaaa gaaagaaaac cagttcaagt agatcctccc
     1081 gtactaaaaa ccattcggga tcttagttat gcaaaagata ttcctatgta tgaaatagtc
     1141 aaacttgcaa tggacgccta tgttgatact ttaaacgacg aggaacggat tatatataat
     1201 agacgatcaa aagatt
//
LOCUS       DACCXM010000259         1211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.269, whole genome shotgun sequence.
ACCESSION   DACCXM010000259 DACCXM010000000
VERSION     DACCXM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.269"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            67..1023
                     /locus_tag="IVY61_002891"
     CDS             67..1023
                     /locus_tag="IVY61_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971934.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1077..>1211)
                     /locus_tag="IVY61_002892"
     CDS             complement(1077..>1211)
                     /locus_tag="IVY61_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7971935.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          206 g          399 t
ORIGIN      
        1 acagggaatc ctaaaaacat tcggcttttt ttatataatc tttttaactc aacagagata
       61 agttttgtgg aattgtatac gctacctata attataaaaa cagaaaaaat gttaaaagat
      121 ttaaaacagt attatcctct gtctgcttat gaaaaaaaga aattattcca ctttttagca
      181 atcaacctgt tggctgaaca agctgattct ttagatattg ctagcaatca gtcgctattt
      241 ttttcggata taggatttta ccacttaaaa ggagtattag gagaaaaatc cgttattttt
      301 atcaataaga cactctcttg gctttatctt catgataaat tggatcaaaa atacttgaga
      361 aaaaatgaag atgaaacaat cgaatggttg aatgaacgat tcattgaaag ttttgaaaat
      421 cagtttaatc tgttgcgaga agagacgagc caattgatcc gaagagagtt aaccaaaata
      481 cattttaaac ttttgcatta cactattaat aaagctgatg aatttccact tgatgtttct
      541 ttttttagag aaaactatcc agcattttat tgctatttga tagagtatat aaatgatcta
      601 acgtcaaaac gcaaagagtt gcttcaagat aaattttttc tttttttcag ttatttatta
      661 ttattgatca atcacatacc cattcaactg atttctgaac cagtaaagat catcatcgat
      721 ttttcttatg gtgctgccta taatcaattt atcaagaaaa atttaagctt gtacattaat
      781 ctgaacacgg aagtgataga ggcaggaaag cctgagttcc cggatgttat aatcacgaat
      841 aggaataatc tatatgaaga aagtgcatct caagtagtgg tttggctcga tccgccaaga
      901 gcagtagact gggggaatct tacaaagatt ttgttgacta tccaagaaga gaaatacaaa
      961 gacagcaaaa ttgacaagca aatagacgat tttcctgaag aaatcatcga caggatcgaa
     1021 tagtgaactt cttttagaaa gtccgactgc tttttctaaa aatattattt gtcaaattaa
     1081 gcgagacaaa actttccatt tacgtaactc aaaaaaactt ctaatggtgt ttggtaattt
     1141 agtgattttc tagggatatg gtttcttttg gacgcaacgg atgagatgaa tccttgattg
     1201 acttggttga a
//
LOCUS       DACCXM010000260         1184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.270, whole genome shotgun sequence.
ACCESSION   DACCXM010000260 DACCXM010000000
VERSION     DACCXM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.270"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..383
                     /locus_tag="IVY61_002893"
     CDS             <1..383
                     /locus_tag="IVY61_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7971936.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
     gene            complement(367..960)
                     /locus_tag="IVY61_002894"
     CDS             complement(367..960)
                     /locus_tag="IVY61_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP7971937.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
BASE COUNT          356 a          237 c          210 g          381 t
ORIGIN      
        1 catttagccg tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa
       61 aagagtttga taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat
      121 tgagtcataa aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga
      181 aagtagaatt tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat
      241 ttgactatgt aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac
      301 cggttggtta ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg
      361 ctaacgctag cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc
      421 gaggaggctc attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat
      481 aaaatttgtc aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc
      541 ttcaatgtcc atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa
      601 tctttggatg acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg
      661 aattgttgaa gccatacgtt cttctatggg tttatcaata actgttaccg gtacaacttt
      721 taagttgagt ctttccttag caacacgata cctatgaagc acatcaacca agatatactt
      781 ctcttctttt tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga
      841 aagctccaat aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg
      901 tattttggta attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat
      961 acgcttgtat accctacttt cttaaaaagt agggtatact caagaatccg attaattatc
     1021 ttctcaattt ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc
     1081 tgcaaaatgg gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa
     1141 tttgtatcct aaaaaatcca taaaaattct ctcacatttt cggg
//
LOCUS       DACCXM010000261         1170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.271, whole genome shotgun sequence.
ACCESSION   DACCXM010000261 DACCXM010000000
VERSION     DACCXM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.271"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            28..501
                     /locus_tag="IVY61_002895"
     CDS             28..501
                     /locus_tag="IVY61_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP7971938.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            510..737
                     /locus_tag="IVY61_002896"
     CDS             510..737
                     /locus_tag="IVY61_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971939.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          369 a          192 c          200 g          409 t
ORIGIN      
        1 gctatcaaag ataaaagagg gataaaaatg aaatttaaaa cattgctatt aaaatttgtc
       61 gttttaggta taagtgccgt atttattttg ttcggcgtac ttttttatac tcagatcgct
      121 acaagcgaaa aaggattcaa ttttgattgg cagaccacat tatttgtttc tgtcatttac
      181 tttgcccttc tcctagggat cgttattgct tatttcttat tgaaactatt gaaaaaaatc
      241 gaagataacc aagctttttc cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc
      301 attttgttta ttggtttcgc cttttttggc ttacttccta aatttatgaa agtagcggat
      361 ttagacgatg ctccaggttt gatgttggta ggaatcgctg tcgtattcat tccttttgca
      421 gtatacacat taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt acttaagaca
      481 gatcatgatt tgacagtgtg aggtgcgaca tggcaattat cgttaattta gatgtagtat
      541 tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt acgatggcaa
      601 acatctctat tctaaaaaat ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa
      661 tctgccaagt tttggagtgt caaccaggtg atttgttgac ctataaaaat gactcgaata
      721 ctataccaga agaataaaat aaagtagaac aaacagtaaa taaaaaataa tctgaatgat
      781 agatgtgtga gacttttaga agttatttac gtctattatt cagatttttt atttcgattg
      841 aataccgttt tatctataaa taagatatgt actgactcat acaaaggggg ccttttcttc
      901 tccatgtact atcacttttc ctgatacaac tggtttcctg ttacgttgta gttggatctg
      961 cttttagtat aatactttat tcattccacc agactgatag cagtttgagc agtctaaata
     1021 atcatgtagt attacaggcc attctgtttc ataccttgaa gtgcttaaaa cttcagctaa
     1081 tggcccattg tgttaactct tactccattc ttttttagta agctcccata tatactgtaa
     1141 agccttctat atattatgtc atgttgcttt
//
LOCUS       DACCXM010000262         1161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.272, whole genome shotgun sequence.
ACCESSION   DACCXM010000262 DACCXM010000000
VERSION     DACCXM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.272"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..386
                     /locus_tag="IVY61_002897"
     CDS             <1..386
                     /locus_tag="IVY61_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7971940.1"
                     /translation="VCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMMEAMAS
                     GLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNENLSRI
                     KEFEVSIVKKRIKEIYEEVIGESVK"
     gene            383..>1161
                     /locus_tag="IVY61_002898"
     CDS             383..>1161
                     /locus_tag="IVY61_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7971941.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNV"
BASE COUNT          457 a          132 c          233 g          339 t
ORIGIN      
        1 aagtatgtga tagaaaagag ggattctgtg gcttcagaat agatatcaaa gaattggcaa
       61 aagtttctga tattttttta tttactacat tacaagaagg aatgccaaga tctatgatgg
      121 aagctatggc aagtggattg ccatgtatag caagtaaaat ccgaggtaat gtagaccttt
      181 tagtggaagg aaaaggtgga tatcttgttc cagctaaaga tgcagaatta gtagcagagt
      241 gtttgagaga actaatcaat aataaatctc ttagaaagta tatgggaaat gagaaccttt
      301 ccagaattaa agaattcgag gttagtatcg taaaaaaaag aatcaaagaa atatatgaag
      361 aagttatagg tgagagtgta aaatgaaaaa tccaatgttt tcaataatag tacctatcta
      421 ttgcgttgaa aaatatttga aagattgctt agattcaata agaaatcaga catttaaaaa
      481 ttttgaagca atactagttg atgacggtag tcctgacgaa tgcccagaaa tatgtgacta
      541 ttattcccaa atagatggac gtttaaaagt aattcataag aaaaacggtg gaataataga
      601 tgcacggaaa acaggagtca ctagtgctat gggagagtat atagtttttc tagatggtga
      661 tgattggctt gcaaatactt atttagaaaa atatgcggaa gtaattacta agtacaatcc
      721 agatataata tgtagtgggt attttgcatc acgttccaac aaaattagaa aaagagaatt
      781 gtcttgctcg tttggatatt atgacaaaga taaaattata aatttgattt atcctaaatt
      841 aattgaggat gaacatggag agtattttag accacaatta tgggcaaaag cttttagaac
      901 tactctgtac aaggaacaac aacaagtatc tgggcaagtt agtgttggtg aagatcatgc
      961 atgtacaaaa ccatgtattt accatgcaaa ttcgatatat atgatggaaa aatgtttata
     1021 ttattacaga ataaacgaaa attctataac aaaaaaacca caaccatatt attgggaagg
     1081 accaaaggct attggagagc attttgaaaa gcagattgat atgaataagt atgattttca
     1141 agatcaagta tatagaaatg t
//
LOCUS       DACCXM010000263         1161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.273, whole genome shotgun sequence.
ACCESSION   DACCXM010000263 DACCXM010000000
VERSION     DACCXM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.273"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..969
                     /locus_tag="IVY61_002899"
     CDS             <1..969
                     /locus_tag="IVY61_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7971942.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          394 a          204 c          232 g          331 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt a
//
LOCUS       DACCXM010000264         1157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.274, whole genome shotgun sequence.
ACCESSION   DACCXM010000264 DACCXM010000000
VERSION     DACCXM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.274"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            41..472
                     /locus_tag="IVY61_002900"
     CDS             41..472
                     /locus_tag="IVY61_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971943.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            474..611
                     /locus_tag="IVY61_002901"
     CDS             474..611
                     /locus_tag="IVY61_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP7971944.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            648..941
                     /locus_tag="IVY61_002902"
     CDS             648..941
                     /locus_tag="IVY61_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971945.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            938..>1157
                     /locus_tag="IVY61_002903"
     CDS             938..>1157
                     /locus_tag="IVY61_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7971946.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT          434 a          167 c          239 g          317 t
ORIGIN      
        1 tcatttgtta tcacttattc aatttaaggg aggaaatcta atgaaagtag tttacaaatc
       61 aatcaagcct tacggattcg agcaaatcat tttgaacaat caagaaaata tccctgaaaa
      121 ctgtacagag attaaaccac cagttcctaa ctggagacca agatttgatt ttgataaaaa
      181 acagtgggtt gagttagcta ctgaagaaga gaaaaaaggg aatgctgttg atagcgtaga
      241 tgaattggct aatattaagg cattatacga aacgctgaag gcagaaaatg atgaattgaa
      301 acagctaaat tctaaagcaa tgctaaacaa tgtagcaatc aagcaagaaa atgtcttatt
      361 gaaagaaaag tcagaaagtt tagcgcagtt gaattcaaaa acgatgcttg cttctgtaca
      421 aaataccaag gaaatcgaag aaatcaaaaa acaattacaa ggtgggaagt aacatgtatt
      481 catatgaaga tatcaaacta atgtatgact ggggctgttt cactaacgaa caagtaatgg
      541 ttttcgttcc attatgcatt acagacgaag aagcagataa aatcattaat aaagataaga
      601 gcgcatctta attgatgcgt ttttatttaa ggtaaaggag ttgtcacatg attaatttag
      661 gggaatgggg aatgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa
      721 ttagaccgat tgctgcatct ttctcgaaaa ttactgagac tctttcaaaa gtaagccaca
      781 atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg
      841 aagaactaaa gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca
      901 cgcaacaaat caaaactttg tttagggaga gaagaaaatg aataataaaa cgttcgaagt
      961 actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg
     1021 taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc
     1081 gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag
     1141 cgaagaagga ggaaaca
//
LOCUS       DACCXM010000265         1126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.275, whole genome shotgun sequence.
ACCESSION   DACCXM010000265 DACCXM010000000
VERSION     DACCXM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.275"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..87
                     /locus_tag="IVY61_002904"
     CDS             <1..87
                     /locus_tag="IVY61_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP7971947.1"
                     /translation="FKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            92..1039
                     /locus_tag="IVY61_002905"
     CDS             92..1039
                     /locus_tag="IVY61_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7971948.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          360 a          170 c          239 g          357 t
ORIGIN      
        1 tttaaatggt tagaattttt gtttcctaaa tttttagaag attcttcgga gcttcaagat
       61 atctataatt atttatatcg gaggtaaaag gatgagctgt tatcttatca ccggtggggc
      121 tggcttcatt ggttctacct tagccaatca tttaggagaa gaacatgtgg taatcgtcgt
      181 cgatgactta tcaatgggaa aagtagaaaa tcttgatatg acgaaaaata tcacttttat
      241 tgagggagat gtcgctgaga aatccttgat ggaagagatc atgaaagcta ataaatttga
      301 ttatatcttt catttagcag cagttgctag tgtggcagat tcagttgccc gaccagtaga
      361 aacacatcgt gtgaattttg aaagtgtatt aatgatttta gaattgatcc gcaagtatca
      421 acctgattta aaacggatcg tgtttagttc ttcagcggct gtttatgggg atgaaccgac
      481 tttaccaaaa aaagaagagt ccgtgattcg cccattgacg ccttatgcgg ttgataaatt
      541 cgctgcagaa caatatgtgc tagattattg tcatttatat gatgtacccg gtagcgcagt
      601 tcgttttttc aatgtctatg gtccaaatca aaatccgaat tctccataca gtggtgtgat
      661 ttctattctc gtagatcgct ataagaaaca aatcgtagga gaagcaactt catttacatt
      721 atttggtgat ggaagccagt ctagagactt tgtttatata gatgatgtaa tccaagcatt
      781 acttttagtt gcgaatgagg aagctgcact ggggaaacaa ttcaatgttg gaacgggtaa
      841 agcaaccaca cttttggaat tgattcatgc aatcaatgaa attctcggtg tagaattgac
      901 acttgaatac aaagaagagc gttcgggtga tatccatgac tctctagcag atattactaa
      961 gatccagtca attggctatc aaccgaaata tggtatttta agtggaatga ctaattattt
     1021 gaaaactgaa atcaattaaa acatactaag ataagattag agggtaaatt caggctcttt
     1081 gtcaataagg actgatgagt tttgtaaaat caaatctggg atagaa
//
LOCUS       DACCXM010000266         1123 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.276, whole genome shotgun sequence.
ACCESSION   DACCXM010000266 DACCXM010000000
VERSION     DACCXM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.276"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            4..963
                     /locus_tag="IVY61_002906"
     CDS             4..963
                     /locus_tag="IVY61_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7971949.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          383 a          209 c          224 g          307 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gat
//
LOCUS       DACCXM010000267         1122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.277, whole genome shotgun sequence.
ACCESSION   DACCXM010000267 DACCXM010000000
VERSION     DACCXM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.277"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            56..1096
                     /locus_tag="IVY61_002907"
     CDS             56..1096
                     /locus_tag="IVY61_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP7971950.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          463 a          167 c          192 g          300 t
ORIGIN      
        1 gaaaagagta ggtaatttta cataaataaa acaacgtgaa agtagaggat ctacaatgac
       61 tgattttaaa ttttttaagg cggatagagt ttacaacgaa ttattttatc aatttccaaa
      121 agtctttatt gtttctgacg aatacaaaaa aatgaaagat tcaactaaga ttgcctatat
      181 gcttttaaaa gcaagattag agatcgcaat cagcaaacgg caaatcgatg aagaaggtaa
      241 tgtttatttt acttatacga caaatgaact atgtagagta ttaaactgcc aaaaacaaaa
      301 agcgatagca atcaaaaaag agttggaatc ctttggttta ttattacaaa agcagatggg
      361 atttaacaaa cagttaggga aaaataatcc taatagacta tatctagcag aattaaaagt
      421 ctcagaaaat gatatctact tactcgaaaa atttgataga gagaataggg aaaacgttga
      481 taaatcagag ggtatgaaaa tcatacccac cctcgacgaa aaatcagacg ctgaatccct
      541 tggggctcaa gagggtatga aaatcatacc gtgccaaaac gttgataaat cagagggtat
      601 gaaaatcata ccagaactta ataataatat attagacact aatagacaca atatagacac
      661 tgaaaaagac cgcctacaag atcaattgtt gttagacaat tttgagacaa ttatgacaaa
      721 cgacagcatt gctacgtttg tccctgaacg agtattaaat ttgataaaaa cattttcttc
      781 aagttacagt gaagctcaaa aaaccgtcca gactattcat aatgcaaaga aaaaagctga
      841 aatagaaagt gatatttcga tagtttttga agaactcgat agttattatg tcaatgcaga
      901 acaagaatta tacacgacac tgttaaaagc ctatcaaaaa ttaaaaaccg aaaaagtcga
      961 aaatatccag aacctgattt ttgtctatgt aaaaaattgg tttatcgaaa aaccaatagc
     1021 tgctaaagta tcaagtgaaa aacgtttgaa ttatgcaagc tccccaagca ctattacgaa
     1081 agactggtta gagtgaaact ttactgacaa agtgattgtt gc
//
LOCUS       DACCXM010000268         1097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.278, whole genome shotgun sequence.
ACCESSION   DACCXM010000268 DACCXM010000000
VERSION     DACCXM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.278"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..763
                     /locus_tag="IVY61_002908"
     CDS             <1..763
                     /locus_tag="IVY61_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7971951.1"
                     /translation="EKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKIT
                     EEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKE
                     LYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLE
                     PMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSL
                     DEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            835..>1097
                     /locus_tag="IVY61_002909"
     CDS             835..>1097
                     /locus_tag="IVY61_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971952.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTT"
BASE COUNT          486 a          126 c          185 g          300 t
ORIGIN      
        1 ggaaaaaatc ataaaaatat ttgataaaat taataattta ttcaagaatg acaatgctga
       61 attaaggaag ggtcttttag agtttaaact tcaagaacta gaacttcatt actcttattt
      121 agtgaaaatt accgaagaaa aagaacaaca aaaagcaata aaagaacaga tgatagaaga
      181 ggaaaaagtt cgtagagaaa ttgatcgtga aaagaaacgt attgataaag aacaacgtca
      241 attcaattct gaaatttcca aattaatcgc atatatgcaa aaagctaatg cagatgtgga
      301 aaaagaatta tatgcaaata aaattcaaga actggaagaa aaattaaaag aattagaagt
      361 tatacgtgaa aatgtattac aaagagaatt aaatactaga gccggttatg tgtatgtaat
      421 tagtaatatt gggtcttttg gtgaagacat atataaaatt ggtatgacta gaagattaga
      481 acctatggat agaataaaag aattgagtag tgcatcggtt ccatttgaat ttgacgttca
      541 tgcgatgatt ttttcagagg acgctccgtc tttggaaact aaattgcata atcattttag
      601 aaaacaagaa gttaataaaa ttaatcaaag aaaagaattt ttcaaagttt cactagacga
      661 aatagagaaa gttgttttag aaaattataa tggaacagta acatttacaa aattagctaa
      721 agctgaacaa tatagacgat ctttagaatt gtcaaaagat taaatatata aaaagccttc
      781 gggctttttc ttttaacaat taataggaac gtatattcga aaggaataga aataatgtgg
      841 ttagaaaaaa taaatgacaa aaaatttaag tacaccgaac gttatacgga tcccttaact
      901 gaaaaaaaga gaaaagtgtc cgttacttta tctagcaatt cacgccaagc ttggaatcaa
      961 gctaacttac ttttgaatga aaaaatcgca gaaaaaataa aacgaaatga agaattacca
     1021 ctaacttttg gagaattaaa aactaaatgg gatgaaaaat acaagccaac tgtaaaagaa
     1081 agttcctatc gaacaac
//
LOCUS       DACCXM010000269         1054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.279, whole genome shotgun sequence.
ACCESSION   DACCXM010000269 DACCXM010000000
VERSION     DACCXM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.279"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IVY61_002910"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IVY61_002910"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="IVY61_002911"
     tRNA            203..276
                     /locus_tag="IVY61_002911"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="IVY61_002912"
     tRNA            316..405
                     /locus_tag="IVY61_002912"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="IVY61_002913"
     tRNA            420..491
                     /locus_tag="IVY61_002913"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="IVY61_002914"
     tRNA            507..579
                     /locus_tag="IVY61_002914"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="IVY61_002915"
     tRNA            589..661
                     /locus_tag="IVY61_002915"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="IVY61_002916"
     tRNA            697..772
                     /locus_tag="IVY61_002916"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="IVY61_002917"
     tRNA            774..854
                     /locus_tag="IVY61_002917"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="IVY61_002918"
     tRNA            861..934
                     /locus_tag="IVY61_002918"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="IVY61_002919"
     tRNA            937..1009
                     /locus_tag="IVY61_002919"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
BASE COUNT          250 a          234 c          285 g          285 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga cttt
//
LOCUS       DACCXM010000270         1044 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.280, whole genome shotgun sequence.
ACCESSION   DACCXM010000270 DACCXM010000000
VERSION     DACCXM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.280"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            474..716
                     /locus_tag="IVY61_002920"
     CDS             474..716
                     /locus_tag="IVY61_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP7971953.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            713..>1044
                     /locus_tag="IVY61_002921"
     CDS             713..>1044
                     /locus_tag="IVY61_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7971954.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYG"
BASE COUNT          382 a          138 c          209 g          315 t
ORIGIN      
        1 tttccgccat gcttgcaagg gggcggttcg cttcggcggt tcggcgagct tgcgagccgt
       61 tcaaagcccc cgttatctaa caaggggggc acaaaaaaca agaaacacta tacactaaca
      121 agcggaaaac gctgtattta taaggcaaaa ccccattttt acagtagtga tataaattgt
      181 acctatatca ctatgaaaat tgaatagtaa atatgagaaa gacagttgat ttcaaaaaat
      241 aagaaattga ttgtcgtttt cttttgcaga aatttaagtg caggcaatta tggtagttgt
      301 cgtaattgct gtcagctcag tgtttatcac tgatggtgtg aaagatttac ggttggagat
      361 gttcaataag attattggtg cttaattatt tttattgtag tgctgatatg agaatgttat
      421 aattggggta taaaacatac aaataaattt gctgaaaaat gaggtatcaa aatatggaaa
      481 atgaattact ggaaaactta ggaagacttc acacaactga acttggcgtt attagaatta
      541 aaaagaactt atctttgaat gtagaaaatg taattgaatg gtgcaaagaa aaaattagcc
      601 tttctaatgc ggaaatcaca agaaaaggaa aaaactggta tataacgatt gataattgta
      661 ttataacggt aaatgcgtac agttataccg ttatcactgc tcacaaggta aaatgataaa
      721 tttttctata aaggaattaa acgcatttgc agttattggg caggaagtag aactgactaa
      781 ttatcaaaaa aagaatattc agattagtac acagttttgg agaaagttta acagtagttt
      841 gaaaaaagcg tatctttcac aatcaggaaa ttgggtaaaa tatgctttta tggaaagaag
      901 aaatggaaaa ctttattatt tctgttctat tccccaaaga accattaccc cagagggctt
      961 tctgtataaa gaaataccgt cttataaata tttggttgtg gagcatattg gtgctatgga
     1021 taaaatatat gaaacttatg gcaa
//
LOCUS       DACCXM010000271         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.281, whole genome shotgun sequence.
ACCESSION   DACCXM010000271 DACCXM010000000
VERSION     DACCXM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.281"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>1035)
                     /locus_tag="IVY61_002922"
     rRNA            complement(<1..>1035)
                     /locus_tag="IVY61_002922"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACCXM010000272         1018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.282, whole genome shotgun sequence.
ACCESSION   DACCXM010000272 DACCXM010000000
VERSION     DACCXM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.282"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            24..434
                     /locus_tag="IVY61_002923"
     CDS             24..434
                     /locus_tag="IVY61_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7971955.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            448..804
                     /locus_tag="IVY61_002924"
     CDS             448..804
                     /locus_tag="IVY61_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7971956.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          348 a          156 c          198 g          316 t
ORIGIN      
        1 tataattaaa aaggagtgag aaaatgataa aagtatatac ttcgcctagc tgtacttcct
       61 gtagaaaagc aagagcatgg ttgcaagcga atcaacttga atttgaagag aagaatattt
      121 ttgccgaacc gttaacagaa agtgagatca aaaagatctt ggcagcgact gaaggaggag
      181 ttcaagatat tatttccact cggtcgaaaa tttacgaaaa gctgggcata gattttaacg
      241 aattaacttt aaagcaaatg atcgtcttat tcaaagaata tccttcatta ttgagacgac
      301 ctattttgat tgatgaaaaa agaatactaa tcggatttaa tgaagatgag atccggtcat
      361 ttgtccctcg tgaaattcga tgtgttgcca gaaatctgat ctatttacaa aacaataaca
      421 tcttaaattc atagggaggg agccttcgtg gaagtgaaag tctatggatc agcgcactgt
      481 gtaacaagtc tgaaggcgaa acaatggtta gaacaccatc aattgcctta tcaatttgtt
      541 gacttagaag aacgcgattt aagtacagaa gaagcagaag aattatgcag ttttgaagaa
      601 gtgcaagcag aacagctatt tgcgacttgg tctgaggctt ttcacaacat tgcgattgac
      661 ctttcctgtg taggaaaaaa acaactgatt tttttatgca cacaacatac caatctcctc
      721 agacgtcctt tgattattat cgatgaagtt ctatttattg gatacaatga gcagttgatg
      781 gaacagctta tcttaaataa atagacttta actgttttaa tcaagtcact gagcatttca
      841 tacagttgta acagtcgaac acttagcatc aagcgtgtta tgtgttcttt ttttgacttt
      901 atttttctac tatagagaca tgctcaattt atgtggtcaa aattcaaatc taggtgggga
      961 tatagagtta aaaatatatt gacatgtgta aatttacatg atatattcat ttctgttg
//
LOCUS       DACCXM010000273         1001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.283, whole genome shotgun sequence.
ACCESSION   DACCXM010000273 DACCXM010000000
VERSION     DACCXM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.283"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            370..1001
                     /locus_tag="IVY61_002925"
                     /pseudo
     CDS             370..1001
                     /locus_tag="IVY61_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DACCXM010000274          997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.284, whole genome shotgun sequence.
ACCESSION   DACCXM010000274 DACCXM010000000
VERSION     DACCXM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.284"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(48..121)
                     /locus_tag="IVY61_002926"
     tRNA            complement(48..121)
                     /locus_tag="IVY61_002926"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(85..87),aa:Ile,seq:cat)
     gene            complement(676..990)
                     /locus_tag="IVY61_002927"
     CDS             complement(676..990)
                     /locus_tag="IVY61_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971957.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          370 a          157 c          154 g          316 t
ORIGIN      
        1 actcttcaaa aaattctaac acagaacgat tcaattggct acattaatgt accctgtagg
       61 actcgaacct acgaccggac ggttatgagc cgtctgctct gaccaactga gctaagggta
      121 gtggttgttg ccacataaag ccataaacaa tcaaccagta gaatgcgtgg caacaaacct
      181 gttatcgcat atcttggagt gtgactattt atgagtgata gtgaagatat gcgtctccat
      241 atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt
      301 attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc
      361 aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg
      421 tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa
      481 ggaaaaacac aattatggtt taagcatgtt ccttcaaata agttttttaa ttataaactc
      541 tagagaaatt aattcatcct aacataaaat aattgttcac tcaaaagttt ttctagaaca
      601 attaaaacag caggcttcac attcgctata catttgtaag aagtaataat tcctgtacta
      661 aaaggcataa ttgctttaat tattttttga cttctcttca aacacaagaa gtagttgtgt
      721 aataattgca gaaataagga ttccaataat agatataaaa tttgacatgg aaacgtctat
      781 aacattaaaa gaataaagtg ttgaagaaag taaactgatt gaaataaccc ctccgtaaaa
      841 aatagtctgt gaatataccc actttttttc agaagacgta cttcgtttgg ttctaaaacc
      901 tatccctgca tttctactct tatcaaaaaa ccaaatattt agaagtaata atattgctat
      961 tattaagata aaattactgt aaaaatccaa aagctat
//
LOCUS       DACCXM010000275          962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.285, whole genome shotgun sequence.
ACCESSION   DACCXM010000275 DACCXM010000000
VERSION     DACCXM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.285"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(210..758)
                     /gene="aac(6')"
                     /locus_tag="IVY61_002928"
     CDS             complement(210..758)
                     /gene="aac(6')"
                     /locus_tag="IVY61_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7971958.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          280 a          204 c          143 g          335 t
ORIGIN      
        1 caaatttatg caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact
       61 aagcgacttt ttcacaacct ctggtacacg gtgttccaaa agtagcttct tcggaaataa
      121 gttggaattt cacaaaaatt caagaagtaa ttttcgtaaa ttccttctta tttcttgaag
      181 ctgattactt ctgtcacaac ctctttttat tattgagaat ttggtcgagg aataatcgtt
      241 tttgccatcc aaatatccgg tttgtcccag ccatttgcat ttggtaagac acctacgatt
      301 ttataaccta atttttcata gaattcatac ggatgttcac gaaggttctg gatggaagcc
      361 actttatcaa atgtatgctc atacaggtcc gtttgactta acgttgttcc atggtctaaa
      421 tcatccgtac ctaaataaat cgtgattcct cctctggaag ctacttcttt ttctaagtaa
      481 ttgactaatc gagtacctat ttggttcttt cgtcgggagc tttctacaac taatggatgc
      541 aattcccaac ctgtgatacc gtattgaggg attgcaccaa taaatcctac taactcatct
      601 tggtctaccg ctgctaccgc gattcgttct ggattcatca tttcttctac ttcttctgcc
      661 gagctgtctc catattcttc cggccaagtc agtctcagta aatcagaaag ctgatctttc
      721 aatactggat tattacggtc aaattcactg attatcatac gtcttccccc ttgttcttgc
      781 ttattctaac agtccttttg aaaaaacgaa atgttctcgc tcaaaaatct ttagctacca
      841 atcagtatct ctattctttc attatttatc ttacttataa aataaaaaat tatacagaaa
      901 cactttattc aaaataggaa agatcaggaa agccatcaat tggaggaaaa aactctgttt
      961 gt
//
LOCUS       DACCXM010000276          959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.286, whole genome shotgun sequence.
ACCESSION   DACCXM010000276 DACCXM010000000
VERSION     DACCXM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.286"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(107..511)
                     /locus_tag="IVY61_002929"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="IVY61_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="IVY61_002930"
     CDS             complement(570..>959)
                     /locus_tag="IVY61_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7971959.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DACCXM010000277          929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.287, whole genome shotgun sequence.
ACCESSION   DACCXM010000277 DACCXM010000000
VERSION     DACCXM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.287"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            23..>929
                     /locus_tag="IVY61_002931"
     rRNA            23..>929
                     /locus_tag="IVY61_002931"
                     /product="23S ribosomal RNA"
BASE COUNT          286 a          180 c          276 g          187 t
ORIGIN      
        1 atcaaaaccc aaccgtaagg ttgataaggt taagtgaata agggcgcacg gtggatgcct
       61 tggcactagg agccgatgaa ggacgggact aacaccgata tgctttgggg agctgtacgt
      121 aagctatgat ccagagattt ccgaatgggg gaacccaaca tctttaatag gatgttacga
      181 ttgtgtgaat acatagcaca ttcgaggtag acgcagagaa ctgaaacatc taagtacctg
      241 caggaagaga aagaaaattc gattccctga gtagcggcga gcgaaacggg aaaagcccaa
      301 accagcaagc ttgcttgttg gggttgtagg actccaatat ggtagttctt tcagatagtc
      361 gaatgacttg gaaaagtcag tcaaagaggg taaaaacccc gtagacgaaa tgtggaagac
      421 acctaggagg atcctgagta cggcggaaca cgagaaattc cgtcggaatc cgggaggacc
      481 atctcccaag gctaaatact ccctagtgac cgatagtgaa ccagtaccgt gagggaaagg
      541 tgaaaagcac cccggaaggg gagtgaaata gaacctgaaa ccgtgtgcct acaacaagtc
      601 aaagcccgtt aatgggtgat ggcgtgcctt ttgtagaatg aaccggcgag ttacgattgc
      661 atgcgaggtt aagttgaaga gacggagccg cagcgaaagc gagtctgaat agggcgtttg
      721 agtatgtagt cgtagacccg aaaccatgtg atctacccat gtccaggttg aaggtgcggt
      781 aaaacgcact ggaggaccga acccacgtac gttgaaaagt gcggggatga ggtgtgggta
      841 gcggagaaat tccaaacgaa cttggagata gctggttctc tccgaaatag ctttagggct
      901 agcctcggaa ttgagaatga tggaggtag
//
LOCUS       DACCXM010000278          919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.288, whole genome shotgun sequence.
ACCESSION   DACCXM010000278 DACCXM010000000
VERSION     DACCXM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.288"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            32..886
                     /locus_tag="IVY61_002932"
     CDS             32..886
                     /locus_tag="IVY61_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7971960.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
BASE COUNT          344 a          126 c          155 g          294 t
ORIGIN      
        1 aattatccaa atctaacata ggagggtgga tatggaaaat tctatatatt tagtaacaga
       61 acctaacact cagctgaaag attgttactt taatttttgt gggtattcaa aaacagaacc
      121 tagacatagt tttggaccag ctattcgaga acaatactta atacacatag tgctagaagg
      181 tgaaggctat tattcaatta atgatcaaaa atattttctg aagaaaggcc aaggatttgt
      241 tatcccacca ggagtatcta ctttttatca agcagatgaa aaaaatccgt ggtcatatgt
      301 atggttgggt ttaagtggca atcttgtaga atactatcta aaagaaattg gtgttggaaa
      361 gaatcatctt acctttaaag tcgaaaattt aaattcattt aaaggattaa tttttgaaac
      421 tttatcgtat gatcaggaca atctagtcag tgaattgtcc agacaaaaac aagtttataa
      481 atttttagaa ctcctcttaa attcagctag ttatctgaac gaagggataa ttactaaaaa
      541 gatgaatcct tttgttcgtc aaacaattga aataatacgt gagaatgcgt atcaaaaatt
      601 atctgttaaa gatattgctg tcagtctatc gatacacgaa aattatttat cacgattgtt
      661 taagaatgat atgggaatat caataaaaga gtatattagt gaaatcaaaa tttctattgc
      721 aaacgatctg ttatcatcaa ctgaatattc aatccagcaa atcagtgaca agataggatt
      781 ctcaacagta caatcttttt ctaaagcctt taagaaagca aatggtgttt caccgggagt
      841 atatcgaaaa cgtagattag gtctaggaat cattaatggt gaataaacta aacatttgat
      901 tttgataaat attcgttac
//
LOCUS       DACCXM010000279          900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.289, whole genome shotgun sequence.
ACCESSION   DACCXM010000279 DACCXM010000000
VERSION     DACCXM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.289"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            71..>900
                     /locus_tag="IVY61_002933"
     CDS             71..>900
                     /locus_tag="IVY61_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP7971961.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          315 a          134 c          164 g          287 t
ORIGIN      
        1 atagaaaaaa ggaagtccct tctgtagaat agagttacca caacacattc acagaaaaga
       61 ggacttccat atgaacgatt ttactacaga aattatggaa atactaatca ataaaggtga
      121 tttggatgat ttgtttcgtc gtcacctaga attggctatt aacgctttat tacaagctga
      181 attaacggta tttcttgact atgaaaagta tgatagagct ggatttaatt caggtaattc
      241 ccgtaatggg aattattcgc gctcttttaa aacagagtac ggtgaattga atttagtaat
      301 acctagagat aggaatggag acttttctca acaaacttta cctgcttata aaagaaccaa
      361 tgactcttta gaaaccacta ttattcagct agttcaaaaa ggaattacta tgtccgaaat
      421 ctctgatcta atcgaaaaaa tgtatggtca ttattacaaa ccacaaacta tttcaaatat
      481 gagtaaaatt gtgtctgaag atgtttcggc atttaaagaa agaactttag aagctaaata
      541 ctcagtcatt tttatggatg ctactcatat tcccttaaaa agacaaaccg tatcaaaaga
      601 agctgtctat atagtaattg gtatcaggtt ggatggaact aaagaggttc taggatttac
      661 tatagctcca actgaatctg cttatgtttg gaaagagatt cttcaagatc taaaagaccg
      721 tggtttagaa gaggttttgt tagtcgtaac agatggttta agtggtattg acgatagtat
      781 tcatagtgtt tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat
      841 cgctcataag gttcgtgtta gtgatcgaca agaaatctgt agcgatttca aattagttta
//
LOCUS       DACCXM010000280          876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.290, whole genome shotgun sequence.
ACCESSION   DACCXM010000280 DACCXM010000000
VERSION     DACCXM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.290"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..873)
                     /locus_tag="IVY61_002934"
     CDS             complement(<1..873)
                     /locus_tag="IVY61_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP7971962.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISE"
BASE COUNT          272 a          162 c           98 g          344 t
ORIGIN      
        1 ctctgaaatc aaaatatagc gtgatggata ctcatcaaaa gaagaatcgt attcttcctt
       61 taaaattccc gatttactta atttttctag tacatcaaat actctatcac tcaagtaact
      121 ccttaaattg ttatcacttt cccattgaac tacttttcta taagttgaat tatcagttga
      181 aaagatagaa ttttcatcca taaacgcata tattagcagc aatctttcat cattgtcaaa
      241 ttctattgga tcaaaaatat ctaagttgtc atcaaaatct ttatcagttc cggtaacttt
      301 tttgttaatt actgaaggtg tcttgtaaga aatagaatct aaatcaaagc gttttgattc
      361 taaattattt tgtgctttct caattaaata attcactttt tttgtgagaa accttctgat
      421 attgtctttg ctatatgttc cataaaaacc cttatcttct aaagagtccc tgtacttctt
      481 aacttctata tactcttcag ccaaaacttg tggaactggt tcatctaaga aaaaaacccc
      541 tacctcgcct ttgctatttt tataaaagta atcaatttct gctatagttc ccgatctata
      601 attatcaact ggagaaccca acttatggcc aaaaatcgct aataatatat cactattttc
      661 tacaatttgt ttattaataa cactttgccc agagctatta tttatataag taggaatcgc
      721 attcgtttcc catcgaattg gcatcaaaat tacttttgta ttatctgtat tttctatatt
      781 ccatctatat atggtttctt ctacttcatt cctctttgct gctatatcac ctggtgatgc
      841 tataaatact tttattagat ttatggtttt cattta
//
LOCUS       DACCXM010000281          870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.291, whole genome shotgun sequence.
ACCESSION   DACCXM010000281 DACCXM010000000
VERSION     DACCXM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.291"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          290 a          142 c          104 g          334 t
ORIGIN      
        1 atacaataaa taactttagt tactttaatg cgttcttact ttatttttat attatcattt
       61 ttcttattca cttctttggt ttatactatt cttataaata aaaatgaggt atgtcctatg
      121 atgaaaagat tgtactattc attgatcata acgattggct atttgattgt atcaaatcta
      181 ggaaatatgg tatttggtat cagtaaagaa ttcagttgga ctactacatt atgggaatct
      241 ctattctttt tcatcttcgt atttttgtta caaaattatc gaaaaaaata aacatgtgcc
      301 atctatctgt tggcacatgt tttaggattt atggtacttt ttattcggca atccatttta
      361 tttttctact tcttcttaat acctctaaca cttttgattt ataaactaat ccaatgcatc
      421 tacttgcgct tattaagcta tttcagctag atagtaccac cccatgcgtc aatgcttgat
      481 tttccttttg tataacattg tataagcaat aaaggacaaa taagtggtga ttacaccaaa
      541 ccattgaata agaaaaattc tgatacgtaa ataaacatga acttgaaaaa attaatgaat
      601 cggttagcta ctacgtagtt gtcttttttc tcatacagtt ctgttaactt acacttctct
      661 tcgctagata ataaaaaatc ctccaactgt attgtccaat ataatcccag gcatctaaac
      721 tagcattata gaagagacac acatagaatc taaaataact ggttttaaat gtttcttaca
      781 atcaaaaaaa gctgttaaat gcactccaca ccttcatact ctatcatttt ttataaacga
      841 tatattttta ttgtttttcg ataaaaaata
//
LOCUS       DACCXM010000282          870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.292, whole genome shotgun sequence.
ACCESSION   DACCXM010000282 DACCXM010000000
VERSION     DACCXM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.292"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..626)
                     /locus_tag="IVY61_002935"
     CDS             complement(<1..626)
                     /locus_tag="IVY61_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7971963.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          268 a          164 c          165 g          273 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat
//
LOCUS       DACCXM010000283          869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.293, whole genome shotgun sequence.
ACCESSION   DACCXM010000283 DACCXM010000000
VERSION     DACCXM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.293"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            515..869
                     /locus_tag="IVY61_002936"
                     /pseudo
     CDS             515..869
                     /locus_tag="IVY61_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          323 a          153 c          147 g          246 t
ORIGIN      
        1 aagttttact ttgattttta aatagaaaga catttgggtt aaattgtgag ataataataa
       61 agaagagttt aaagcgcacc ccaaaccact tccccataag tgtgttacgc tttaaactct
      121 tttatatttg aagccattaa aaagcatacc atatttttga aaaaaagtga gaaaaaaggc
      181 ttacaattgg agtggtagtt aattagtgac ttatttttga ttttatagca ctgatactat
      241 aaaatataga tatcatcata ttacacaatc ttaatactaa cttaaaaaat atctcctttc
      301 acaagtatgg tgataaaatt cgttccgggc taccttttta ggtagcctac tttaatcttt
      361 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat gatcgctacg
      421 tgcgtccggc acgtcagtca tgaatcggct acactagact agacagaaaa aataaggtgt
      481 gtagaataat aaaaaacaca ctggaggatt tttcatgtca agacgtcaac gaagaaccta
      541 ttcaaaagaa ttcaaacaac aaatcgtcga tctctatctc gctggtaagc ctcgcgcaga
      601 aattattcga gagtatgagc ttacgccttc ttctttcgat aaatggatga agcaagcaca
      661 atcaacgggc tcattcaaag aaagagacaa cttaacacca gaacaagcag aattgatcgc
      721 actaagaaag aaaaataagc aactcgaaat ggagaatgat attttaaagc aagcggcgct
      781 gatattcgga cgaaaagaca agtaattgat gccaacaagc ataaatattc catatcagcg
      841 atgtgcaaaa ttctaaatat ttctcgtca
//
LOCUS       DACCXM010000284          852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.294, whole genome shotgun sequence.
ACCESSION   DACCXM010000284 DACCXM010000000
VERSION     DACCXM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.294"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..116
                     /locus_tag="IVY61_002937"
     CDS             <1..116
                     /locus_tag="IVY61_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP7971964.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
     gene            244..795
                     /gene="raiA"
                     /locus_tag="IVY61_002938"
     CDS             244..795
                     /gene="raiA"
                     /locus_tag="IVY61_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP7971965.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT          306 a          151 c          177 g          218 t
ORIGIN      
        1 aaaaaatatt gattattgac gatgtctata caacaggaca gacgcttttt catgcagcag
       61 attgcttgca gaacgcacag ccgaaaatca tccgaaccat ttctttggca agatgaatca
      121 gatgtggaaa tttgatagcg atttaatttg caaataacct agagttcgat ataatagaat
      181 taagaagagg gaaagagtgg tccttctcct tcttgattgt ggtagacgaa aggggtaatt
      241 tttatgttta gatataatgt acgcggcgaa aatatcgaag taactgaagc gattcgcgac
      301 tacgttgaga aaaaagtagg caaactagaa cgctatttca gtgacgcacc cgatgcaacg
      361 gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa aagtggaagt aacgatccca
      421 ttaccttatc ttgtattacg tgccgaagaa acttctccag atttatacgc aagtatcgat
      481 ttggttgttg acaaattgga acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa
      541 tcccgtgaaa cgggcatgaa tactgcagat gctgctgctt tattcaatga agaggacgaa
      601 aacgaaaatg attctgaatt agatatcgta cgtacgaaac gcctttcatt gaaaccaatg
      661 gatagtgaag aagcagtctt acaaatgaac atgctgggac ataacttctt tatttttgaa
      721 gatgcagaaa caaatggtac aagtatcgtt taccgccgta aagatggaaa atacggactg
      781 attgaaacag actgatcagc cttacttaaa taacaacgaa aacttgcagc agctactgac
      841 agctgccgca ag
//
LOCUS       DACCXM010000285          847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.295, whole genome shotgun sequence.
ACCESSION   DACCXM010000285 DACCXM010000000
VERSION     DACCXM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.295"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            74..688
                     /locus_tag="IVY61_002939"
                     /pseudo
     CDS             74..688
                     /locus_tag="IVY61_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          310 a          134 c          151 g          252 t
ORIGIN      
        1 acattgaaaa atttagagtt ctaacgtatg attagggtgt attataaacg ttatagaaaa
       61 gtagggaata aatttgaaaa ttgcttatat tagagtttca tccatcgatc aaaatgaaca
      121 acgtcaaatt gaagaaatga aaaagtttgg cgctgaaaaa atttttatcg aaaaacaatc
      181 tggagcgacg attagtcatc ggccgatttt ccaggaagct ttagattttg tcagagagca
      241 agatattttt attgtagaag ccattgatcg acttggacgc aattatgacg agattatcga
      301 ttcagtgaat tatctaaaga agaagaatgt gcgacttatt atcaccagtt taccgatcat
      361 ggctgaagca attggaaatc ctctcctaga taaattcatc aaagacttaa tcatttaaat
      421 ctcagccatg attgctgaac aagaacgtac tgaatcgaaa cgacgccagg cacaaggaat
      481 taaaatcgct aaagcaaacg gcgtctataa aggtcgtcct aagttataca gtgcagacac
      541 caaagatcct caacgtcgcc ttgtctatag gagtatcgtt caggatcttg agaatggggt
      601 tgctatctca aagattgcaa cagactacaa tgtgactagg caaacaattt accgaataaa
      661 aaaagaaatc gaccaactaa ttgtatgatt tcttttttga atcacgtaat tattaattag
      721 ctttgtcatc aatacagaca atcattttag attcatctag ctaagttgat tacatttatg
      781 ttaaaaagaa tttagttaac ataaatataa ttgatgcata aatcgtcctt atttaaaggc
      841 atttaac
//
LOCUS       DACCXM010000286          842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.296, whole genome shotgun sequence.
ACCESSION   DACCXM010000286 DACCXM010000000
VERSION     DACCXM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.296"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>842
                     /locus_tag="IVY61_002940"
     CDS             <1..>842
                     /locus_tag="IVY61_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP7971966.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLK"
BASE COUNT          367 a          108 c          136 g          231 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 tt
//
LOCUS       DACCXM010000287          798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.297, whole genome shotgun sequence.
ACCESSION   DACCXM010000287 DACCXM010000000
VERSION     DACCXM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.297"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>798
                     /locus_tag="IVY61_002941"
     CDS             <1..>798
                     /locus_tag="IVY61_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7971967.1"
                     /translation="KPDMVIAYTIKPNIYGGLACRLRKVSYALNITGLGTAFQKKGVL
                     KSLIVFLYRLAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEY
                     PKDEQVHFLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEK
                     EGWLKYHGYQSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAV
                     IESKSGYLCERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKF"
BASE COUNT          304 a           89 c          159 g          246 t
ORIGIN      
        1 taaagccaga tatggtcatt gcttatacta tcaaaccaaa tatatacggt ggattggcat
       61 gtcggttaag aaaggtttct tatgcattaa atataaccgg attaggaacg gcatttcaaa
      121 aaaagggggt tttgaaaagt cttattgtat ttttgtatag attagcagct aaaaaatcta
      181 aggtcatatt tttcgaaaat tttgaaaata tgaaaacatt tcagacttta caaattgcta
      241 aacaaagtaa atacaagcta ttaaacggtg caggagtaga tgtagaacac tttagttatt
      301 tagaatatcc taaagatgag caagttcatt ttttatttat tggaagaatt atgaaagaga
      361 aaggtgtaga tgaacttttt gaagcaatga aaaacttata tagtgaagag aaaaattgtg
      421 tacttgatgt ccttgggtat tttgaagaag attacggcat aaaaattgaa aaatacgaaa
      481 aagagggatg gttaaaatat catggatatc aatctgatat tcgtcccttt gtagaaagag
      541 cacattgctt tgttttgcct agttggcacg aaggaatggc aaatactaac cttgaatcag
      601 cagcatcagg gagaccaatt attacaagtg atatcccagg gtgtaaagaa gcagttattg
      661 aatcgaagtc tggttattta tgtgaaagaa aaaatagttg tagtctctat aattcgatga
      721 ataaatttct aaaattatca tatgagcagc gcattaacat gggaaaaatg gggagaaatt
      781 atatgattaa aaaattta
//
LOCUS       DACCXM010000288          769 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.298, whole genome shotgun sequence.
ACCESSION   DACCXM010000288 DACCXM010000000
VERSION     DACCXM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.298"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..615
                     /locus_tag="IVY61_002942"
     CDS             <1..615
                     /locus_tag="IVY61_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP7971968.1"
                     /translation="NLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLG
                     MDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQ
                     FIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNH
                     TISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          271 a           89 c          142 g          267 t
ORIGIN      
        1 aatttagaaa acatggtttt taatctgagt aatggaataa ttccaagtgt tagtggagat
       61 acgattcggt ctgaaaaaaa ttattctatc attgtttttg aaaagttagc acaaacgtct
      121 ataacattag gaatggatat aatagaagcg tatcaaagta gagatgcttt gatacaagaa
      181 aacgaattag ctgtatcgtt acctgaggtc ttaaaagtga gggattctgg gattgtttat
      241 tatactaagg aaatcggaaa aactaagata gagcatcttt ctcccttaat ttcttctgtc
      301 gttcaattca tcggcttaaa tatctataaa agaattactg taaaggaaat agctaattat
      361 ttttctgtaa gtgaaactaa attaagagaa agctttaaga atgaaatgca tattacgatt
      421 tataattata tctctaagcg aaaaatttct acggctaaaa taatgttgaa atcaaaccat
      481 actatcagtg aagtttcttt aggtttagga ttttcagatt cttcacactt ttcgagagta
      541 ttcaaaaagt acgcaggtgt atctcctaag caatatcaat taggtttagt ggacaatttc
      601 aacaacatta gctgagaggt tttattttat tatgagacta tatcatttac gtagtttgga
      661 ggagttgagg aaattggatt ttaacaaaaa atttttttgg gaaatttctg atttgagttg
      721 tttcgtagaa tctgagcgtc taagtaatgg taataatagt gtgttaaat
//
LOCUS       DACCXM010000289          761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.299, whole genome shotgun sequence.
ACCESSION   DACCXM010000289 DACCXM010000000
VERSION     DACCXM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.299"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>761
                     /locus_tag="IVY61_002943"
     CDS             <1..>761
                     /locus_tag="IVY61_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7971969.1"
                     /translation="IKDATDRASKETFANITNSFSMEINRQVNPGTPRGGGNVKGEDI
                     KKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDST
                     KETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKG
                     ADETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFF
                     VKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPA"
BASE COUNT          330 a           86 c          143 g          202 t
ORIGIN      
        1 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
       61 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
      121 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
      181 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
      241 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
      301 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
      361 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
      421 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
      481 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
      541 gagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
      601 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
      661 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
      721 gagaatataa tttgattaaa agttcatcaa attaccctgc a
//
LOCUS       DACCXM010000290          704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.300, whole genome shotgun sequence.
ACCESSION   DACCXM010000290 DACCXM010000000
VERSION     DACCXM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.300"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            23..646
                     /locus_tag="IVY61_002944"
     CDS             23..646
                     /locus_tag="IVY61_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971970.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          244 a          110 c          152 g          198 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaa
//
LOCUS       DACCXM010000291          704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.301, whole genome shotgun sequence.
ACCESSION   DACCXM010000291 DACCXM010000000
VERSION     DACCXM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.301"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            3..359
                     /locus_tag="IVY61_002945"
     CDS             3..359
                     /locus_tag="IVY61_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7971971.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            349..615
                     /locus_tag="IVY61_002946"
     CDS             349..615
                     /locus_tag="IVY61_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971972.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
BASE COUNT          276 a          100 c          150 g          178 t
ORIGIN      
        1 gtatggaaaa aatatctttt gaaacattta aaagacctaa tggtcatgat gaatttttag
       61 aatggttgga aacattacca aaaaaagata gtgcgaagct tttacgtaca atagaagaaa
      121 cagaaaagaa tgggatgcta gtagctcaac ggttgaaatg ggtgaaaaaa ttagacacta
      181 atttgtacga attaagaagt aaggtaggat caaacatcca aagagctatc tatttccatg
      241 tggtcaatgg aaggtatgtc attacccatg gatttactaa aaaaacggag aaaacaccaa
      301 taagtgaact taaacatgca aaagaactta gagaggagtg gttcacaaat gatgattaaa
      361 aaattgattg aagaaaagca aaaagacaaa gattttgcag aagcatatga ccgtgaagga
      421 agaaaactcg aaactgcggt tgctctttat catgcacggg aggaagcagg cctaactcaa
      481 gcggagcttg cacaaaaagc acatacaact caatctacta ttgctcgaat tgagagaggg
      541 gataacgtat ctttcgataa acttgatgat atcgctaggg cattaggcaa aaagctggta
      601 gtagaatttg cttaaaagcg agttgaaaat atacctgtaa taggctccta actaatcaat
      661 tagtttagtg ggcctttttg tactaaatag ataaaagttg tcct
//
LOCUS       DACCXM010000292          702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.302, whole genome shotgun sequence.
ACCESSION   DACCXM010000292 DACCXM010000000
VERSION     DACCXM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.302"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(47..>702)
                     /locus_tag="IVY61_002947"
     CDS             complement(47..>702)
                     /locus_tag="IVY61_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7971973.1"
                     /translation="LFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALLLHNLF
                     GFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFAVDSLL
                     INSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGILVVSL
                     LYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLEQLEH"
BASE COUNT          275 a          126 c          124 g          177 t
ORIGIN      
        1 cataatgata tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag tgctctaact
       61 gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca
      121 aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc
      181 taagtaatgt atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac
      241 caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt
      301 gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa
      361 tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta
      421 tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc
      481 ggagaaaagc gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa
      541 gcagtgaagt gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag
      601 taaccccacc atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga
      661 ttcctgcgcc taaaatgatt ccgcacaatt ctgtaaataa at
//
LOCUS       DACCXM010000293          678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.303, whole genome shotgun sequence.
ACCESSION   DACCXM010000293 DACCXM010000000
VERSION     DACCXM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.303"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            7..654
                     /locus_tag="IVY61_002948"
     CDS             7..654
                     /locus_tag="IVY61_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7971974.1"
                     /translation="MLVIFSGKQYADNILLWGGEDNIEKINNNLTILDTALENKEQKI
                     SILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLENQLSAKDSELQG
                     KQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLNQQINQMLSEK
                     NNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNEHTIQ"
BASE COUNT          321 a           86 c          100 g          171 t
ORIGIN      
        1 ggattgatgt tagtaatttt ctccggaaag caatatgctg ataatatttt attatgggga
       61 ggggaagata atattgaaaa gatcaacaac aatttaacta tattagacac agctctagaa
      121 aataaagagc aaaaaattag tattttaaat tcacaattgt cttcaaacaa ccaaactctt
      181 gaacaactaa gaactaatat tgaagtttcc aaacaaaaag tggcatcttt agaaaacgac
      241 aagaatcaat tagtatcaga gaaaactaat ttagaaaatc aattgtcagc aaaagactct
      301 gaattgcaag gaaaacaaaa tgaaattaat gcgaaaatag acgaaattaa ccaaaaagta
      361 gctgaaataa atcgattaaa tagcgattgg aataatcgtt tacaaaatga aaagaacaaa
      421 tttaatgaag aacaatctac tattaatagt ttaaaccagc aaattaatca aatgttgagt
      481 gaaaaaaata acattcaaaa tcaactgcaa gctactcagc aaaagctaga agaagaaaag
      541 tcaaaaaata gtagtttgaa taattacatt gaaaagcttc aaaaagctaa aaatgatgta
      601 gaggataccg caaataaagc acaacaaatt gtaaatgaac atacaatcca atagatatag
      661 ttttaattta acaaatgg
//
LOCUS       DACCXM010000294          649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.304, whole genome shotgun sequence.
ACCESSION   DACCXM010000294 DACCXM010000000
VERSION     DACCXM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.304"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>649)
                     /locus_tag="IVY61_002949"
     CDS             complement(<1..>649)
                     /locus_tag="IVY61_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HAP7971975.1"
                     /translation="GDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIGIYILN
                     VITKARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNGIYPFA
                     NTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNGGYIGG
                     IVYIFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIVKNF"
BASE COUNT          240 a          122 c           82 g          205 t
ORIGIN      
        1 caaaattttt cacaattaaa tatgaagcag ctctaggaaa acccattata atatttgctg
       61 tagttatagc caaaaacatc ttcataggat gattttcaat atcttggttg tctatactat
      121 tagaaaggat tttaccgatg actataccaa taataaaaat atagactata cccccaatgt
      181 atccaccatt gacataggct tctgcaatca ttgagaatcc agtcccccaa gtgattccta
      241 aataatcctg agaaaaccaa tcagctaata aaacaaactt tattccaaac attgatagca
      301 ttcttgttga tatagagcct aagagtgagg tcaaataagt attagcaaag ggatatattc
      361 cattaccact tattaggaaa aaagttataa tactcatagt cccccccatt tcaaaaaaag
      421 aatcaactaa aggagaatca tctgaatcaa atacagatgt aaaatctttt aaagtaagtt
      481 tacttagtcc tccaccatca cgtgctttcg ttattacgtt tagaatatat attcctataa
      541 cagctaaaat aagccacttt attaattttt tataattaaa ggttccttta tattgagtgt
      601 atgcccatat gagtataata gagctataca accaacttcc tctgtctcc
//
LOCUS       DACCXM010000295          637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.305, whole genome shotgun sequence.
ACCESSION   DACCXM010000295 DACCXM010000000
VERSION     DACCXM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.305"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          222 a          114 c          101 g          200 t
ORIGIN      
        1 ataaaaaaac tagttacaac cttaggacgt tgtaactagc ttgaagcata ctagagctaa
       61 ctaaaatagc actagaaagc aatgttaaac ccgtccaaca aacttgattt aacattgaga
      121 ccagcttatt cagctaaata tagttgaata agcttctttt gttttaaaaa aacaaaaggt
      181 gtactacgaa taacgcaagt aaaagaaata ctattacaac cagtaggcta caagtaagta
      241 ttactaaaca ttgcgtagtt agaacctttg ctctattgtc taaagccaca aaattagaga
      301 tcagctttat gatgctaaga gcgagctgca tccatgcgga cataggttct tttgacacta
      361 ttaattctcc tttttaggat aagggaaatt gctagtagtt ttttcattag caccatcccc
      421 cttcatttga ttgagtctag tgctgctgtg atagtttgct gtttaatatg cttacattct
      481 tattataaca taaatgaaac aaacacatgg ttagaggtcg tatcaatcaa aatagtgtct
      541 cacaaacgat ttattactta ctcctgctga ctaacggact gttcttcagt aactgtaact
      601 atatgtttgt taaaaccacc attacctgta gataaaa
//
LOCUS       DACCXM010000296          635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.306, whole genome shotgun sequence.
ACCESSION   DACCXM010000296 DACCXM010000000
VERSION     DACCXM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.306"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            15..557
                     /locus_tag="IVY61_002950"
     CDS             15..557
                     /locus_tag="IVY61_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP7971976.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
BASE COUNT          201 a          101 c          130 g          203 t
ORIGIN      
        1 aaggagtttt acaaatgatt gatccaaaaa tgttggggat gatcttcctc attaattttg
       61 cttatatcac tctgaacacg ttacgtttca tgttgacaat gaaaggctat cgactgattg
      121 ctccacttgt cagtatggtc gagatcacga tttatatcgt cggcttatcc cttgttttag
      181 atcgtttaga taatccactg aatttatttg tctatgcgtt aggttacgca gtaggtatca
      241 gtgtcggaat tttgattgaa gacaaattgg cattaggata catcatggtc acaacgattt
      301 tgccagcaaa tagcgcagaa gataaaagtt tgccgggtat actgagagaa cacggttatg
      361 gtgtgacaca gacttctgca atggggctgg aaggtgagcg gctagttctc gaaatacttt
      421 ctcctagaaa aagcgaacgt gaactgtatc gcctgatcaa agaaatcgaa gagcgtgctt
      481 ttatcatttc ttatgagcca aaatatattt caggtggttt ctggactaaa aaagtgagaa
      541 aacgacaagg aaaataactc acaaacttaa aaactgtcta atatctaaat taaattttaa
      601 atgttagaca gtttttattt attattttga ttaaa
//
LOCUS       DACCXM010000297          625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.307, whole genome shotgun sequence.
ACCESSION   DACCXM010000297 DACCXM010000000
VERSION     DACCXM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.307"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            1..625
                     /locus_tag="IVY61_002951"
                     /pseudo
     CDS             1..625
                     /locus_tag="IVY61_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DACCXM010000298          600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.308, whole genome shotgun sequence.
ACCESSION   DACCXM010000298 DACCXM010000000
VERSION     DACCXM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.308"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..122
                     /locus_tag="IVY61_002952"
     CDS             <1..122
                     /locus_tag="IVY61_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7971977.1"
                     /translation="ANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            123..>600
                     /locus_tag="IVY61_002953"
     CDS             123..>600
                     /locus_tag="IVY61_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP7971978.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIEN"
BASE COUNT          228 a           71 c          113 g          188 t
ORIGIN      
        1 ctgctaatga tatgtatgag aaggagttga aatatttctt tgaaaacgta gtaacaggca
       61 aagaaaactg gaatgattta tatcatgcaa acgaagtatt gaaaatagct gaggagaaat
      121 aaatggaaat tttatttact atttgtggta gagctgggtc taaggggttt aaaaataaaa
      181 atttgaaata tttggcagga aagcccctag tccatttcgc tttagcaatg atagatatct
      241 atcaacaaaa acatcctgaa cataattgca cgacttgttt gaatacggac agtaatgagt
      301 tagtagaatt gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt aaacctgaat
      361 tagctacaga tactgtaggg aaaattgaag tgatacgtga ttcattctta caactagaaa
      421 gagaagataa tatatttgat ttaattatag atttagattt gacttcacct ttacgtgaag
      481 tttctaatct tgaagaatta atagatgaac atctaaaaaa tttagataaa gacgtctact
      541 tttcagttgt ttctgcaaga cgtaatccct attttaatat ggtaaaaaga attgaaaatg
//
LOCUS       DACCXM010000299          599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.309, whole genome shotgun sequence.
ACCESSION   DACCXM010000299 DACCXM010000000
VERSION     DACCXM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.309"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>599
                     /locus_tag="IVY61_002954"
     CDS             <1..>599
                     /locus_tag="IVY61_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971979.1"
                     /translation="DWLKIEKGNTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVE
                     DNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDE
                     LDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGKSSGGNDSTILTI
                     PEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRR"
BASE COUNT          216 a          104 c          127 g          152 t
ORIGIN      
        1 gactggctca agatcgaaaa aggcaacaca cgaactccga atattagtga gtataaatat
       61 cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt atgtttggga tctagcacca
      121 gaatatgtcg aagataactt ggccacagat attaaaattt ctgaaattac tggtaaagca
      181 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      241 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      301 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      361 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      421 tattttgtgg ctttaaacgg aaaaagcagt ggtggaaatg attcaacgat actaacaatt
      481 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      541 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaa
//
LOCUS       DACCXM010000300          597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.310, whole genome shotgun sequence.
ACCESSION   DACCXM010000300 DACCXM010000000
VERSION     DACCXM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.310"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..434)
                     /locus_tag="IVY61_002955"
     CDS             complement(<1..434)
                     /locus_tag="IVY61_002955"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP7971980.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT          170 a          129 c           99 g          199 t
ORIGIN      
        1 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
       61 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
      121 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
      181 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
      241 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
      301 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
      361 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
      421 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
      481 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
      541 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaat
//
LOCUS       DACCXM010000301          597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.311, whole genome shotgun sequence.
ACCESSION   DACCXM010000301 DACCXM010000000
VERSION     DACCXM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.311"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(180..545)
                     /locus_tag="IVY61_002956"
     CDS             complement(180..545)
                     /locus_tag="IVY61_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971981.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          232 a           78 c          112 g          175 t
ORIGIN      
        1 attcaatatc tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt
       61 ttggagagaa gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatataaga
      121 aaaacatata tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt
      181 tatggatgcc gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt
      241 ggaactaagt aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag
      301 aagtaacgcc attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata
      361 ttggcaatga ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga
      421 gataaaaata tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag
      481 gcgaaatgca cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt
      541 atcatctttc atcaccacct aacaagtgtt attattttgt tagttttatt gtaaatt
//
LOCUS       DACCXM010000302          547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.312, whole genome shotgun sequence.
ACCESSION   DACCXM010000302 DACCXM010000000
VERSION     DACCXM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.312"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>547)
                     /locus_tag="IVY61_002957"
     CDS             complement(<1..>547)
                     /locus_tag="IVY61_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP7971982.1"
                     /translation="QVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLV
                     YFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRF
                     TIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQ
                     VLVSSGIIAFICIYAFYVLNIF"
BASE COUNT          198 a           98 c           68 g          183 t
ORIGIN      
        1 aaagatattt aacacataga aagcatagat acatataaaa gcaattatac cacttgatac
       61 aagtacttga agaaatccat tatgcatgtt tccatgtgct ctttttagtt cattcacatc
      121 tgtagtatca agatctactt ttttcaaaaa ttttgcatcg ctattcccat agaaatcggt
      181 ctctccataa ccaaataaca aagttggtct tataacttta agcgcagctt tccaaatcgt
      241 aaaacgacca gctgaaattt cggagttatc actagaatgc tgtaatatta cttctttttc
      301 tggaagtgga gttttatctg attcatttga ctctataact tctacttttc tatcaatcga
      361 tacaggaatt gttgctaaaa aatccttagc ataattagta ccaaaagaaa aatataccaa
      421 tagaactaat aatatgctta ccatccattt gatttttaca ggtagtactt ctctaatgct
      481 ctttctaaat tttgaattgg caaacaacaa aagaaataat actaaaaaag aacaaattac
      541 tacctgg
//
LOCUS       DACCXM010000303          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.313, whole genome shotgun sequence.
ACCESSION   DACCXM010000303 DACCXM010000000
VERSION     DACCXM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.313"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>534
                     /locus_tag="IVY61_002958"
     CDS             <1..>534
                     /locus_tag="IVY61_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7971983.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DACCXM010000304          525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.314, whole genome shotgun sequence.
ACCESSION   DACCXM010000304 DACCXM010000000
VERSION     DACCXM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.314"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            4..474
                     /locus_tag="IVY61_002959"
     CDS             4..474
                     /locus_tag="IVY61_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP7971984.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          186 a           98 c           77 g          164 t
ORIGIN      
        1 tttatgtcta tttataattt tgaaactact ttagaagacg gcactgtcta ttcattgaaa
       61 aaatatgcag gcaaaccgat aatcattgtt aacactgcaa caaaatgcgg tctagcacct
      121 caatttaaac aattagaaga actttaccaa gcttatcaga cacaaggtct ggttatctta
      181 ggctttcctt ccaatcaatt taaacaagag ctagattcag cgactgaagc agcagaagct
      241 tgtaaaacca cttatggcgt taccttccct atgcatcaat tgattgacgt aaatggagaa
      301 aatacagatc ctctttttac ttatcttaaa aatgaagctc ctggtactgt cactaaagca
      361 ataaaatgga attttactaa atttttgatt gatagaaatg gcaaagtaat cgaacgattt
      421 gcaccacaaa cttctccatt gaaaatgacc gctgcaattg aatccgttct ttaacttaat
      481 aaaaataaaa aaagatgaac tatttctagt tcatcttttt gcctt
//
LOCUS       DACCXM010000305          497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.315, whole genome shotgun sequence.
ACCESSION   DACCXM010000305 DACCXM010000000
VERSION     DACCXM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.315"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(82..318)
                     /locus_tag="IVY61_002960"
                     /pseudo
     CDS             complement(82..318)
                     /locus_tag="IVY61_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          167 a           83 c           87 g          160 t
ORIGIN      
        1 aaaaagatgt tggtgaagtt caccaacatc aaaaagtata gagccgatag aaggactgac
       61 cttctgtctc agattttttt gttatttcac ttgaatcaat tgattgatca aaaaaatcct
      121 gattttcatg ttctcaaatg atttaaatcc ataagataat cgtttcagtg tcttgatatg
      181 gggaatcttg gcctctattt tgccattaga atacgggtaa atcatagcat tgtaaatacc
      241 ttcttcatac gttaaaaggt tttgaagttt cgtgcgaagt tgtgcatcaa gtgtttcagg
      301 aagatttttt aataacgccg tttgtataat taagcgagac aaataaaaaa gccatttatg
      361 ttacactcca attgtaatac ccgctaaagc atacaaggag agtgaacata aatgacttat
      421 acccatctta caccaaacga gcttgtaatg atagaagcat attttcatca agaaactccg
      481 gttgctatcg ttgcgaa
//
LOCUS       DACCXM010000306          486 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.316, whole genome shotgun sequence.
ACCESSION   DACCXM010000306 DACCXM010000000
VERSION     DACCXM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.316"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..451)
                     /locus_tag="IVY61_002961"
     CDS             complement(<1..451)
                     /locus_tag="IVY61_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7971985.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
BASE COUNT          140 a           92 c           61 g          193 t
ORIGIN      
        1 aatttgtaat aagttcgtat atgttttgcg gtgtttcttg tttcttgtaa ttttccacga
       61 atgtttcatc catgaataaa ttcataaacg cttgaagtaa tcctacttgt tcttttggtt
      121 ctttgattcc taaaaaaaat agattttcta cagtcatttc ctgattatcc cccatttgat
      181 aaaaataaat tgggttcttc agctgcataa caaaaacaaa tggcttttcg acatattcac
      241 attccgaatg tggcagcgcg atatttaaat acttagtaat caatccagta ggaaattctc
      301 tttctcttgc aatcagtcca ttcaaataat tctcattgac gtaccccatt ttttctaatt
      361 gttgtgcagc gattaaaaaa gcttcttctt tattttttac ttctaccgct aattgaatca
      421 aactaggaac gaacatctct ttatatttca ttccaacctc ctcctttttg attaagtttg
      481 ttaatt
//
LOCUS       DACCXM010000307          476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.317, whole genome shotgun sequence.
ACCESSION   DACCXM010000307 DACCXM010000000
VERSION     DACCXM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.317"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(2..196)
                     /locus_tag="IVY61_002962"
     CDS             complement(2..196)
                     /locus_tag="IVY61_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971986.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          162 a           94 c           76 g          144 t
ORIGIN      
        1 gctaccccgg cctaatctgg caaggggagg aggagatata atgaaatacc tttttgattt
       61 tgtttttgca atcaagccac ttgcagatgt agtgtgcagt tacctctgca aatggcttga
      121 ttgcaaaatt gccaaaaaag acagtaagcc tacccgttaa ttctataata tagaaacgta
      181 aaaaaaaccc catcagcctg cacgctgatg gggtttggtg atataatgaa atacctcttt
      241 gttactcata attataccat aattagtcac gaatgcaacc aatagaaaaa actacttaga
      301 acggtagatc atcgctaata acaatgcatt gtccaccaaa tggattttgt gaagcattaa
      361 atacattgtt attaattgtc gctgctttta actaggcctc tccccttcta ttataaaaga
      421 agatatatat catttcttaa ttacctacta ccttaaacca aaatttaagt actacc
//
LOCUS       DACCXM010000308          475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.318, whole genome shotgun sequence.
ACCESSION   DACCXM010000308 DACCXM010000000
VERSION     DACCXM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.318"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          159 a           75 c           78 g          163 t
ORIGIN      
        1 atgctttttc taataacaca aggataatgc aatgaaatat aaaattcatt gtcttctatt
       61 gtcttcgaat cgaagaaaaa gttgaataac cgcgattgat tcactttttg ttttcttgtt
      121 tatttgcttt tatctgcaac ttttgacttt aaaatttctt aactatgaaa acctttcaat
      181 tggtggtata cttataaatg gaatttcgtt atcaacgcgt tcgttgcctt tcagcaagta
      241 tttaaagaaa ggagaatggc tatgacattc tataacaaag atatacaata tcgagtaacc
      301 ttggatacag atttgaatct atttgtcgtt tttgacaaag aaaataccaa tcatgtagcg
      361 acaggtgtta cgatcgaaca agcagtgcaa gaattaaaaa aatctgcata aggagtggaa
      421 accctcatat ggacgattct cgttcttctc tttaaacgca tctactggta gataa
//
LOCUS       DACCXM010000309          471 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.319, whole genome shotgun sequence.
ACCESSION   DACCXM010000309 DACCXM010000000
VERSION     DACCXM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.319"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            110..>471
                     /locus_tag="IVY61_002963"
     CDS             110..>471
                     /locus_tag="IVY61_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP7971987.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKK"
BASE COUNT          187 a           53 c           81 g          150 t
ORIGIN      
        1 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
       61 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      121 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      181 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      241 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      301 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      361 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      421 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa t
//
LOCUS       DACCXM010000310          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.320, whole genome shotgun sequence.
ACCESSION   DACCXM010000310 DACCXM010000000
VERSION     DACCXM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.320"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            48..242
                     /locus_tag="IVY61_002964"
     CDS             48..242
                     /locus_tag="IVY61_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971988.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          198 a           60 c           83 g          121 t
ORIGIN      
        1 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
       61 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      121 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      181 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      241 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      301 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      361 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      421 aatcaaatat acttgtcatt catcatgaaa tatagagact ta
//
LOCUS       DACCXM010000311          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.321, whole genome shotgun sequence.
ACCESSION   DACCXM010000311 DACCXM010000000
VERSION     DACCXM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.321"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..430)
                     /locus_tag="IVY61_002965"
     CDS             complement(<1..430)
                     /locus_tag="IVY61_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7971989.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACCXM010000312          437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.322, whole genome shotgun sequence.
ACCESSION   DACCXM010000312 DACCXM010000000
VERSION     DACCXM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.322"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>437)
                     /locus_tag="IVY61_002966"
     CDS             complement(<1..>437)
                     /locus_tag="IVY61_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971990.1"
                     /translation="VEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNE
                     TYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETL
                     RILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEK"
BASE COUNT          128 a           86 c           46 g          177 t
ORIGIN      
        1 ttttctcttt atcttcacct tcaatctggg gagcatatag tccaatttcc tcccaataat
       61 atgaatctct ttcaattccc atgatggtag ctttggctaa tttcaaatca aataacgatt
      121 ttagaattct tagtgtctca ataaagtctt ctctcttcaa ctctttaatc cattgtgaat
      181 tttctggatt atctaaaatt tcaagttcat cttcattata agtatatttc aaatttgact
      241 tgcttaactc atagataaat gaagtttctt gactcataat tcttttaagt acttgactat
      301 aagtttcgtt tgactttgcc catttcgcta attctaccac tgtattcact aataaatcga
      361 aataatgatg ttctcgttca atagtctcag cattttgaaa atgaaaatca cctttataaa
      421 ctccattctt ttccact
//
LOCUS       DACCXM010000313          424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.323, whole genome shotgun sequence.
ACCESSION   DACCXM010000313 DACCXM010000000
VERSION     DACCXM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.323"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>424
                     /locus_tag="IVY61_002967"
     CDS             <1..>424
                     /locus_tag="IVY61_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP7971991.1"
                     /translation="YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNA
                     YSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCD
                     VGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMN"
BASE COUNT          139 a           61 c           77 g          147 t
ORIGIN      
        1 tatatgataa ccctgtgggc gttcttacca ataatccttc atttgactat caattattta
       61 atttaaacaa ttatcgtgtc ttatcgagtg aaactcctaa aaataatttt tcaaatcaaa
      121 taagtttgaa tgcctatagc cgcggtatgg gagggatagg cttgcctgga gatttatcct
      181 cagtatctcg ttttgttaaa gcgactttta cgaagctgaa ttctgtatct ggagattcag
      241 agtcagaaag tattagtcaa tttttccata tcttaggttc agtagaacaa caaaaaggtt
      301 tgtgtgatgt aggtgatgga aaatatgaat atacaattta ttcttcttgt tgcaatgttg
      361 acaaaggaat ctattattat cgaacatatg aagacagtca aattactgca attgatatga
      421 ataa
//
LOCUS       DACCXM010000314          414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.324, whole genome shotgun sequence.
ACCESSION   DACCXM010000314 DACCXM010000000
VERSION     DACCXM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.324"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(109..>414)
                     /locus_tag="IVY61_002968"
     CDS             complement(109..>414)
                     /locus_tag="IVY61_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7971992.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DACCXM010000315          412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.325, whole genome shotgun sequence.
ACCESSION   DACCXM010000315 DACCXM010000000
VERSION     DACCXM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.325"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            133..351
                     /locus_tag="IVY61_002969"
                     /pseudo
     CDS             133..351
                     /locus_tag="IVY61_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          141 a           59 c           72 g          140 t
ORIGIN      
        1 tagatacaat atcgatcagt tttgaggaaa tgaaattcta gatatttttt gctacataga
       61 gttactttca atatttttta acgtaattag gcagaagtca ctcatcctct ataggtttgt
      121 gatgaaattg ccattgatcc atttaagtaa gagcaaaaaa ctggaggttt atcaggtaat
      181 tggtctaatg gatttaagta tgcggtcacc gtgtatagag ttgcttatga gattcaagaa
      241 aataaagtta ttccagtatt gctatatgga acacataaca atttttatga attagccgag
      301 aacactcgca actttattcg tgtagatgaa tcggcttcgg ttgagttata actttagcta
      361 tatcaatcaa ccgaccgaca tatgttaaat ctatatataa taaaaatacc ta
//
LOCUS       DACCXM010000316          404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.326, whole genome shotgun sequence.
ACCESSION   DACCXM010000316 DACCXM010000000
VERSION     DACCXM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.326"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>404
                     /locus_tag="IVY61_002970"
     CDS             <1..>404
                     /locus_tag="IVY61_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7971993.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DACCXM010000317          370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.328, whole genome shotgun sequence.
ACCESSION   DACCXM010000317 DACCXM010000000
VERSION     DACCXM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.328"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(62..262)
                     /locus_tag="IVY61_002971"
     CDS             complement(62..262)
                     /locus_tag="IVY61_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7971994.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          111 a           71 c           57 g          131 t
ORIGIN      
        1 aattttttcg tacaaaaaaa ccgtcttttc tttcgaaaag acggttaaaa gtcaattttt
       61 attaagcttt aacaatgttt gttgcttgag ggccacgttg accatcttca acatcgtaag
      121 acactgcttg gccttcttct aaagttttga agccgtcacc ttggatagct gaaaaatgtg
      181 caaatacatc atttccatct tcaccagtga taaagccaaa acctttgtct gcgttgaacc
      241 attttactgt accgttattc ataaataaat tcctcctgat acgtatcaat gtacgttttt
      301 tcgttgcaat aaatatttga taggcaaaag gagttttatc tatgtagata tatcgctcaa
      361 attacgtttc
//
LOCUS       DACCXM010000318          370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.329, whole genome shotgun sequence.
ACCESSION   DACCXM010000318 DACCXM010000000
VERSION     DACCXM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.329"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          139 a           52 c           61 g          118 t
ORIGIN      
        1 tttttttcaa aaatatggta tgcttcttag tgaattcaaa tataaaagag tttaaagcgt
       61 aatacactta tggggaagtg gtttggggtg cgctttaaac tcttctttat tattatctca
      121 cagcatgctt aaaaggtcta tggattaagt tatgtaaaaa agtgcctctt taaacgtcct
      181 aaaatccaaa caaggatagc ataatgtaca ctatcgttga tccagaaaac aggtaaataa
      241 attttgcaaa agcaataagt taaaaacatt acaaaaaaga caaggatatc acatcaagaa
      301 aaaatggctc tataatttat gggactgatg aatcagaatt gattcttcag ccccattttt
      361 attttaggta
//
LOCUS       DACCXM010000319          367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.330, whole genome shotgun sequence.
ACCESSION   DACCXM010000319 DACCXM010000000
VERSION     DACCXM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.330"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>367
                     /locus_tag="IVY61_002972"
     CDS             <1..>367
                     /locus_tag="IVY61_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HAP7971995.1"
                     /translation="QEYILGEEYTIDVCLNFDSTPVSIVPRKRIATRGGEILKGQIIK
                     DRELIETTKKMLEKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPISIKSGANSPEY
                     IIKMIDSKKNLRYRENYLDK"
BASE COUNT          158 a           39 c           70 g          100 t
ORIGIN      
        1 caagagtata ttttaggtga agaatatact atagatgttt gcttaaactt tgattcgaca
       61 ccagtaagta ttgttcctag aaaaagaata gcaactagag gtggggaaat actgaagggt
      121 cagattatta aagatagaga actaattgag actacgaaaa agatgttgga aaaattaaat
      181 gcaatcggac atattactat tcaatgtata aaaaacgaaa agggaatatt ttatttagaa
      241 ataaatgcaa gatacggtgg aggagccccc attagcatta aatcaggagc taattcccct
      301 gaatatatta taaagatgat agattcaaaa aaaaatttga gatacagaga aaattattta
      361 gataaaa
//
LOCUS       DACCXM010000320          365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.denovo.331, whole genome shotgun sequence.
ACCESSION   DACCXM010000320 DACCXM010000000
VERSION     DACCXM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.denovo.331"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            19..>365
                     /locus_tag="IVY61_002973"
     CDS             19..>365
                     /locus_tag="IVY61_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7971996.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDK"
BASE COUNT          148 a           62 c           63 g           92 t
ORIGIN      
        1 ataaaaagga gaaaaaagat gaagccaaat ccttcagaag caggaaaacg aatcaaacaa
       61 ttacgccttt catgtggttt tactatggaa gaacttggaa ggaaaataga taattcacca
      121 cgtgcgacca tatccaactg ggaacgtggg accaatttac caaatccaca aaagctgaaa
      181 ttattatcaa ctatcactaa ttcaacaatt gactggatta aatggggaac tttagaagaa
      241 tatataactt cataccttat cgatataggg tatgagctat atattaagga cttcccagaa
      301 atacctcaca aagtctttaa agacattcaa gaaagatata gtgatacgtt ttcgctagat
      361 aaaga
//
LOCUS       DACCXM010000321        34691 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.guided.014, whole genome shotgun sequence.
ACCESSION   DACCXM010000321 DACCXM010000000
VERSION     DACCXM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.guided.014"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..232
                     /locus_tag="IVY61_002974"
     CDS             <1..232
                     /locus_tag="IVY61_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7971997.1"
                     /translation="MIQQDSLFTKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNI
                     NLLESYTMNKPSDNFSFRFAFRAKSKKTSPIK"
     gene            392..2278
                     /locus_tag="IVY61_002975"
     CDS             392..2278
                     /locus_tag="IVY61_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7971998.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2290..3237
                     /locus_tag="IVY61_002976"
     CDS             2290..3237
                     /locus_tag="IVY61_002976"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7971999.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3257..3784
                     /locus_tag="IVY61_002977"
     CDS             3257..3784
                     /locus_tag="IVY61_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7972000.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3847..4500)
                     /locus_tag="IVY61_002978"
     CDS             complement(3847..4500)
                     /locus_tag="IVY61_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7972001.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4632..5474
                     /locus_tag="IVY61_002979"
     CDS             4632..5474
                     /locus_tag="IVY61_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7972002.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5632..6528
                     /locus_tag="IVY61_002980"
     CDS             5632..6528
                     /locus_tag="IVY61_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7972003.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            6531..7244
                     /locus_tag="IVY61_002981"
     CDS             6531..7244
                     /locus_tag="IVY61_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7972004.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            7264..7782
                     /gene="msrA"
                     /locus_tag="IVY61_002982"
     CDS             7264..7782
                     /gene="msrA"
                     /locus_tag="IVY61_002982"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7972005.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            7779..8006
                     /locus_tag="IVY61_002983"
     CDS             7779..8006
                     /locus_tag="IVY61_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7972006.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            8158..8709
                     /gene="lepB"
                     /locus_tag="IVY61_002984"
     CDS             8158..8709
                     /gene="lepB"
                     /locus_tag="IVY61_002984"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7972007.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8792..9334)
                     /locus_tag="IVY61_002985"
     CDS             complement(8792..9334)
                     /locus_tag="IVY61_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7972008.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(9571..9969)
                     /locus_tag="IVY61_002986"
     CDS             complement(9571..9969)
                     /locus_tag="IVY61_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7972009.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            10229..11089
                     /gene="ylqF"
                     /locus_tag="IVY61_002987"
     CDS             10229..11089
                     /gene="ylqF"
                     /locus_tag="IVY61_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7972010.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            11082..11849
                     /locus_tag="IVY61_002988"
     CDS             11082..11849
                     /locus_tag="IVY61_002988"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7972011.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            11905..12762
                     /gene="dprA"
                     /locus_tag="IVY61_002989"
     CDS             11905..12762
                     /gene="dprA"
                     /locus_tag="IVY61_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP7972012.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            12884..14962
                     /gene="topA"
                     /locus_tag="IVY61_002990"
     CDS             12884..14962
                     /gene="topA"
                     /locus_tag="IVY61_002990"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP7972013.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            14931..16307
                     /gene="trmFO"
                     /locus_tag="IVY61_002991"
     CDS             14931..16307
                     /gene="trmFO"
                     /locus_tag="IVY61_002991"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP7972014.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            16505..17410
                     /gene="xerC"
                     /locus_tag="IVY61_002992"
     CDS             16505..17410
                     /gene="xerC"
                     /locus_tag="IVY61_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP7972015.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            17447..17995
                     /gene="hslV"
                     /locus_tag="IVY61_002993"
     CDS             17447..17995
                     /gene="hslV"
                     /locus_tag="IVY61_002993"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP7972016.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            18009..19409
                     /gene="hslU"
                     /locus_tag="IVY61_002994"
     CDS             18009..19409
                     /gene="hslU"
                     /locus_tag="IVY61_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP7972017.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            19427..20218
                     /gene="codY"
                     /locus_tag="IVY61_002995"
     CDS             19427..20218
                     /gene="codY"
                     /locus_tag="IVY61_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP7972018.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            20300..21175
                     /locus_tag="IVY61_002996"
     CDS             20300..21175
                     /locus_tag="IVY61_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7972019.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(21307..21945)
                     /gene="plsY"
                     /locus_tag="IVY61_002997"
     CDS             complement(21307..21945)
                     /gene="plsY"
                     /locus_tag="IVY61_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP7972020.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            22072..22506
                     /locus_tag="IVY61_002998"
     CDS             22072..22506
                     /locus_tag="IVY61_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP7972021.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            22672..24717
                     /gene="parE"
                     /locus_tag="IVY61_002999"
     CDS             22672..24717
                     /gene="parE"
                     /locus_tag="IVY61_002999"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7972022.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            24729..27179
                     /gene="parC"
                     /locus_tag="IVY61_003000"
     CDS             24729..27179
                     /gene="parC"
                     /locus_tag="IVY61_003000"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP7972023.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            27456..29687
                     /gene="pflB"
                     /locus_tag="IVY61_003001"
     CDS             27456..29687
                     /gene="pflB"
                     /locus_tag="IVY61_003001"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP7972024.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            29931..30692
                     /gene="pflA"
                     /locus_tag="IVY61_003002"
     CDS             29931..30692
                     /gene="pflA"
                     /locus_tag="IVY61_003002"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP7972025.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            30777..31703
                     /locus_tag="IVY61_003003"
     CDS             30777..31703
                     /locus_tag="IVY61_003003"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP7972026.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            31885..32778
                     /locus_tag="IVY61_003004"
     CDS             31885..32778
                     /locus_tag="IVY61_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP7972027.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            32912..33037
                     /locus_tag="IVY61_003005"
     CDS             32912..33037
                     /locus_tag="IVY61_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7972028.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(33171..33803)
                     /locus_tag="IVY61_003006"
     CDS             complement(33171..33803)
                     /locus_tag="IVY61_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7972029.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(33951..34493)
                     /locus_tag="IVY61_003007"
     CDS             complement(33951..34493)
                     /locus_tag="IVY61_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP7972030.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
BASE COUNT        12296 a         5987 c         7226 g         9182 t
ORIGIN      
        1 gatgatccag caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag
       61 aaaaatcatt gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta
      121 tcgcaggaat atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc
      181 tttccgattt gcgtttcgag caaagtcaaa aaaaacgagt ccaataaaat aaaaaggtaa
      241 aaattttcaa gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc
      301 tttttttgag aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt
      361 tatgatacaa tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct
      421 caaaaaaact tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa
      481 agaccgtatc gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat
      541 tgccggtatc gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg
      601 tattggttat ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt
      661 ctttcaagga aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga
      721 actaggggaa aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca
      781 aatgaataaa gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt
      841 aggaatcagt gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt
      901 tagtctggcg caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa
      961 ccatttagat tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc
     1021 actattgatg gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga
     1081 actttcattt ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa
     1141 agcagaacgt gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca
     1201 ggaattagct tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat
     1261 caaccgtttc gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga
     1321 gctgaaccta gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta
     1381 ccgtataaat gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag
     1441 actaggtatt actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg
     1501 cagaattccc ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta
     1561 tacacaagaa aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc
     1621 tgctgaagaa gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg
     1681 atttttattt ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa
     1741 acgccggtta tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga
     1801 accgaccaat gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt
     1861 tccaggtgct gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa
     1921 gttattagta ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta
     1981 tttactgaag caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag
     2041 caaggaacct gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat
     2101 tgaagatgag attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca
     2161 tcaaggagat aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc
     2221 acaacttgaa gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga
     2281 ggaaaaagga tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat
     2341 cgtaaagaag acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt
     2401 gatctgcaaa aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa
     2461 agcgaattgc tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa
     2521 aaccatattt gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga
     2581 ccagatatga ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat
     2641 aaaaagcaga gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca
     2701 tacggcgatc ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt
     2761 ggctttatcg atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga
     2821 agattgatcg tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt
     2881 catacgatgt ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc
     2941 agtggggatg tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac
     3001 ctaattgcac atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct
     3061 catctttata tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca
     3121 tttccgactt tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat
     3181 atccaattag aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat
     3241 cagaaaggat gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg
     3301 aaaagaagac cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac
     3361 agaagcaaat acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt
     3421 gccgaatcgt cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca
     3481 tgtcatgcat agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt
     3541 catcgctgga gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg
     3601 tacagtcatc catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga
     3661 ttggtcactg atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca
     3721 atttgaaacg tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat
     3781 ataaaaaagc tatggataga ctagctgtta tttagagctg tcccatccat agctttattt
     3841 tttttattaa gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa
     3901 caaatgccaa acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat
     3961 cgtgtaagaa agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata
     4021 aagaggaaca gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac
     4081 ttttgatact ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc
     4141 aaacaaccag attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca
     4201 aaaaggagta taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa
     4261 cacctttttt gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag
     4321 aatcatcatc gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc
     4381 accttttact aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt
     4441 gactgcatta aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat
     4501 gaaaacggct cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa
     4561 aaatagaaat tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga
     4621 gagagaaaat aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa
     4681 atgtacgcga tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag
     4741 tatatcccga tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa
     4801 aaaatctgcc caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc
     4861 taggagaaga cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag
     4921 tagaagcagc aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg
     4981 ataccactga ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag
     5041 gtaaaacagt agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat
     5101 acatcggaat atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg
     5161 gtttgctgtc tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa
     5221 tccctgtaac taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa
     5281 atgaactgaa agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag
     5341 aattggcgca agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg
     5401 tactgcatac gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt
     5461 attatgtaga ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc
     5521 tctttttctt tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt
     5581 tgataaaatt tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta
     5641 acgaaacgac atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca
     5701 ggtttagctg cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga
     5761 gaagatccaa ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa
     5821 ggagtaggag atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa
     5881 gaccgttata atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt
     5941 gaccagatct taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct
     6001 gattttatca cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta
     6061 ttcgggttaa cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca
     6121 gaactattgg aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc
     6181 tacaatccat tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat
     6241 tggaacaaag gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc
     6301 aatgatttgc tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat
     6361 caaacaacta gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa
     6421 gatcatttcc atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag
     6481 ctgatcgaga cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa
     6541 gatctgacgt atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta
     6601 tccttcttgg ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc
     6661 gggaaccaga actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa
     6721 cgatcaattc aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc
     6781 aaaaagatag tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta
     6841 ctttcgaagt actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg
     6901 aaaacggaaa tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa
     6961 tcaaagatgt gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca
     7021 atacagatga tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt
     7081 atattaaagc agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc
     7141 caaaagatac ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta
     7201 ctgaaagcaa gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc
     7261 ataatggaac gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac
     7321 acgcttcctg gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg
     7381 acttatgaac aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat
     7441 gatccaacat tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca
     7501 actgacgctt tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat
     7561 ttgacagaag aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga
     7621 cgtttcgaac agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa
     7681 gaagaccatc aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc
     7741 cgggctgcct ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt
     7801 atttaatgac actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag
     7861 ccggtaaaga catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc
     7921 tggaactgaa tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat
     7981 atttagaaaa taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt
     8041 tttgtccaat agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt
     8101 tgatattttt cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg
     8161 acaaaaaaac aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct
     8221 gtattccttg cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat
     8281 tcaatggaaa acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc
     8341 agacgatttg atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta
     8401 atcggtcttc caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa
     8461 gtagttaagg aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat
     8521 acgacggact ttacactaca agagctaacg ggacaatcca agcttccgga agataattat
     8581 tttgtacttg gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat
     8641 aaaactgata ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg
     8701 atccagtgag aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc
     8761 caaaatcagt ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag
     8821 cctctaaagt tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat
     8881 tcctgatgaa tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca
     8941 gccaagaatt gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg
     9001 aaggcaggtt tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag
     9061 ccgtgataag aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt
     9121 tcgacaacat ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca
     9181 tacttaaaaa tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga
     9241 tgacatcaag ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca
     9301 atctaaaaac gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata
     9361 ataaaaggat accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg
     9421 gcatctaaca attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt
     9481 gtacgaataa aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt
     9541 gttataaaaa acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt
     9601 atttccgtct agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc
     9661 tacttcgtgg aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc
     9721 cgcattttca acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc
     9781 tttatctttt ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc
     9841 atcccatacc attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag
     9901 cttttcataa aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat
     9961 actattcatt tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa
    10021 cgctgtcaaa aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa
    10081 agtgaagatg tgatagaatg aggactggtt cactttcact taactaatag atggaatttc
    10141 tttataataa aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata
    10201 ttccggtatg aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc
    10261 aaaagcccga cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt
    10321 agacgcacga ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa
    10381 acctcgcctt gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg
    10441 gcaagaatat tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa
    10501 aggaattaaa ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga
    10561 tgctgctcgg gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt
    10621 tgggaaatct acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa
    10681 gcctggcgta acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga
    10741 cacaccaggg attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct
    10801 gacaggcgcg atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga
    10861 cttttttgcc cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca
    10921 agaagaaaaa ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga
    10981 agactatgat cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg
    11041 atacacactg gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa
    11101 gtatcaaaga acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa
    11161 gaaatgatcc acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa
    11221 aggaagcatt aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg
    11281 cacaaggtca ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac
    11341 ctgtcgtggc agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact
    11401 ccaaacaatt atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc
    11461 tggcaatcgg gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag
    11521 caagtaaatt agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt
    11581 tgatcgatgc gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg
    11641 cacgatctgt gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga
    11701 tgaaagaata tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa
    11761 caaaggaaca cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt
    11821 ttgcacctgt aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa
    11881 agagagacga aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct
    11941 tatacaaaag gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat
    12001 ccttataaag aaatcggatt tgaagacatc attaagatag gtaaaataac caaacaccgg
    12061 gagctgtttc gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa
    12121 cagtcgttta tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat
    12181 ccgccaattg tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc
    12241 gttgttggtg gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa
    12301 ccagtgatag aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac
    12361 ggtcaccaag cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata
    12421 gagcaagtct atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt
    12481 cttttgagtg aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat
    12541 cggattatcg cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc
    12601 tctctgataa cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga
    12661 aatattttaa cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc
    12721 acggtttcgg gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg
    12781 cttttcttga caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct
    12841 tttaatagta ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt
    12901 agtgattgta gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta
    12961 taaagtaatg gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga
    13021 ttttgaaaac aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa
    13081 aagcctgaaa gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag
    13141 agaaggagaa gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa
    13201 aaatcgggtg gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc
    13261 aagaacgatc gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat
    13321 cgttggttat tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg
    13381 acgtgtccaa tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt
    13441 tgttccagaa gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa
    13501 agcaaatttc tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa
    13561 agaagtcact gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga
    13621 gcgcaaaaga aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg
    13681 taagttaaac tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat
    13741 cactttgggt aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg
    13801 gattgccgat acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga
    13861 attttctaga catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga
    13921 agctgtgcgt ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa
    13981 agatcaattg aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc
    14041 agctatttta gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa
    14101 cggttcaaaa atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg
    14161 caaagaagag aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt
    14221 tgatattgaa ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt
    14281 gattcgtgca ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga
    14341 tacgatccaa agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact
    14401 aggagaaatt gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt
    14461 cacagcagaa atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa
    14521 agtggttgat cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat
    14581 cgaaaaaatt caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc
    14641 gatggtgata aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg
    14701 tcggaatacg aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg
    14761 acaagtcatt gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc
    14821 agaatgtgaa ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg
    14881 atatctggtc gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta
    14941 cgaagaagac atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg
    15001 ttggagcagg gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg
    15061 tccgtttata tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg
    15121 ctgaactagt atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat
    15181 tgaaagaaga aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag
    15241 tgccagctgg tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa
    15301 aagtcaaaga gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag
    15361 gaatcaccgt agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag
    15421 cattcaatgg ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag
    15481 ctacgatcga tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt
    15541 acttgaactg tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg
    15601 ccgaagtggc agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa
    15661 ttgaagtaat ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg
    15721 gattggaaga tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca
    15781 atgctgcagc ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc
    15841 aaaaacgtgt tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg
    15901 tgatgcaccg aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa
    15961 cgaaacgcga agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa
    16021 gtgcagcgag cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac
    16081 cagtcgagtt tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg
    16141 aaggaaaaca tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc
    16201 gcattcgaga caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg
    16261 aggcaataaa aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga
    16321 agagggtgag acggatagtc tcgctctttt tcttgcgttt ttcttaagaa tttctcaaac
    16381 aaaatgtgag gaattgcgcc aattgtgaac aaacttagaa atattggtta aattctgaca
    16441 atttatttgt ctttttataa acgctgtgct aaattattct ctgggacaaa aggaaggtga
    16501 gaaaatgaca gaagccacgt gggaacaaaa atttttgcgt tatttgattg tcgaaagagg
    16561 atattcagaa aaaacgaaag aagcatatga agaagacatt ctccatttca aacatttttt
    16621 ggaggaatct ggagacgctg atctgctgaa ggcggaatat tttacggttc gtgtctattt
    16681 gagtgcatta gctgatcatc gtcctagcta tagcagaaat tctatcagtc gcaagatagc
    16741 cagcctacgt tcgttttatc agtatcttct caaagaagaa gtgataaaag aaaatccatt
    16801 ttcttacgtt catctcaaaa agaaaaattt gagactgcca cgtttttttt atgaaaatga
    16861 aatgcaagca ctttttgaca gcgtagcagg agatagtcct ttggatttga ggaaccgcgc
    16921 tttactggaa gtcctttacg ggtcaggcat ccgtttaagt gagtgtagca gtttgtcggt
    16981 atcagacatt gattttgatt cagaggtcat gttgatccat ggtaaaggga acaaagagag
    17041 atatgctcct ctaggttctt ttgctcaaga tgctctgcaa gaatatttca gggaaggccg
    17101 ccaggtattg atgacaaaat atcacgaaga gcatgatatc gtttttgtga atcatcatgg
    17161 aaaaccaatc acgcctactg gaattgaata tgtcttgaat caaatgatca aaaaaagcag
    17221 tttggatagt gatatccatc ctcacatgct gcgccataca tttgctactc atttattaaa
    17281 taatggggca gatttgcgga cagtccaaga attgctagga catgccaatt tatcgactac
    17341 ccagatctat gcccatgtaa caaaagaaag tttacaaaaa aactatcgtt ctttccatcc
    17401 aagagcgtaa aaataaggaa aagaacaaat ttaggaggaa acaaacatgg tcgaatcacc
    17461 atttcattca accactatct gtgccgtcga aaaagacggg aaatttgcta tggctggaga
    17521 tggccaagta acaatgggtg aatctgttgt catgaaaggc actgcaaaga aagtacgacg
    17581 tatctacaat ggagaagttg ttgtaggttt cgccggaagt gttgcagatg cgtttacttt
    17641 agaagaaaaa tttgaaggca aactaaacga atataacggg aatctacaac gcgcagctgt
    17701 cgaattagct caagaatggc ggacacagca atctatgcaa aaacttgaag cgatgctgat
    17761 cgtaatgaat gataaagaaa tgcttcttgt ttcaggtaca ggagaagtta tcacaccaga
    17821 tgatggtatc ttagctatcg gatcaggagg gaattttgcg ttggctgcag cccgagcaat
    17881 gaaaaactat gctcaaaacg acatgagtgc gaaagaaatt gcagaaaatg cgttaaatat
    17941 tgccgcagat atctgtatct ttactaatca taatattatc gtagaagaac tgtaagaggt
    18001 gagtagatat gtatgaatta aaacaaacac caagacaaat cgtaagtgaa ttagatgaat
    18061 atatcgtagg acagcacgca gcaaaaaaat ctgttgccgt tgctttgcgt aaccgctatc
    18121 gccgtttgca gttagatgaa aaaatgcaac aagatattac accaaaaaat atgctgatga
    18181 tcggacctac aggggtcggt aaaacagaaa tcgcacgacg tttggcaaaa atcgtcaatg
    18241 caccatttgt caaagtcgaa gcaacaaaat ttactgaagt cggttatgtc ggaagagatg
    18301 tagaatcaat ggttcgggat ttagtagaaa atgcgatcca aatcgtagaa aaagagcagt
    18361 acagccgtgt atacacaagt gctaaaaaaa atgcagaaaa acgtttggta aaaatcttgg
    18421 tacctggtat caaaaaagaa cagaaacaat ccactaatca atttgaacaa atgatgaatc
    18481 tttttaatgc tagtcagcaa aataccgctg aaccgcaaga agaagtgaca gaagatattc
    18541 gaatcaatcg ccgtacagtc ttggaacaac tacaaaaggg tttactggat agtcgtgaag
    18601 taaccattga agttgaagac caaaagaaac ctgcaccaat gatgaacaat ggattggagc
    18661 aaatggggat cgacctaaat gaaactctag gtgctttgac accgaagaaa aaagtggaac
    18721 ggacggtaac agtcaaagaa gctttagagt tattgatcaa ggaagaatca gcaaaattag
    18781 tcaaggatgc tgatatccat agtgaggcaa tcaaattagc ggaagaaagt ggaattattt
    18841 ttatcgatga aatcgataag atcacttcta aatcacagca atctggagaa gtatccagag
    18901 aaggcgtcca aagagacata ttgccaattg tcgaaggttc acaagtaaac acaaaatacg
    18961 ggatcatcca aacagatcac gtcctattca ttgcttcagg agctttccat ttgtctaaac
    19021 caagtgatct aattccagaa cttcaaggac gcttcccgat acgggtagaa ttagatgatt
    19081 tgacagctga agattttgtg aagattctga ccgaaccaaa taatgcttta gtaaaacaat
    19141 acgtcgcgtt gatcggtaca gaaaatgtgt cagttacgtt tacaaaagaa gcaattgaac
    19201 ggatcgctca aatcgctttc gatgtcaacc gtgacacaga taatatcggc gcacgcagac
    19261 tgcataccat tttggaaaaa ctactagaag atctattatt tgaagcacca gatatgcaaa
    19321 tgggagaaat aacgatcaca gaagcatatg tggatgacaa acttggctca attgcggcaa
    19381 atgaagattt aagtcgctat attttataaa aaatcggagg acgacaatga cagacttgct
    19441 aacgaaaacc cgcaagataa acacactttt gcagcaaaag aatacttttg acatgcaagc
    19501 agatttgcct tacgataaaa tggctgtcac actaggtggt attcttgaca gcaacaccta
    19561 tatcatagga agtgacggag tattactagg ttataatgaa cgtcacgatg tgaacaatga
    19621 acgaatcaaa acaatgtttg ttgagaaaca atttcctgaa tcttatactg atacagtggc
    19681 acagttgatg aaaactgaag ctaatatccc aattactagt gatattactg catttccggt
    19741 agagtcaaga gagaaatatc catttggtct gacgactgtc gttccgatct ttggagctgg
    19801 cgaacgtttg ggtacgatta tcttatctcg tatggaacaa tcgtttgcag atgacgatct
    19861 agtcttagca gaatatggag ctacggttgt cggtatgcag atcctttatc agaagtctcg
    19921 aagcatcgaa gcagatgtac ggagtgcaac ggctgtccaa atggcaatca acacattatc
    19981 ttatagcgaa ttgaaggctg tacaagctat ctttgaagca ttggatggag atgaaggtcg
    20041 tctgactgct tctaatattg ccgatagtat cggcatcact cgttctgtga tcgtcaatgc
    20101 tttacgaaaa ttggaatcag ctggaatcat tgaatctcgt tcgttaggca tgaagggaac
    20161 ttatttaaaa gtattgaatt cccgttttaa agacgagtta gcaaaacaca gttattagaa
    20221 gtaaggggac tggggcagat agaaatgttt gttccagcct tcccatttta aaaagtaagt
    20281 cgaagttgga ggaatggcaa tgactgttat tattcaaaat gaccagctga tcgccgaaat
    20341 cgctgaacac ggtgcggaat tgatcagtct gaaatcaaaa gaacatggtt tagagtatat
    20401 ctggcaaggt gatccagcgt attggggcag acatgcaccg gttttattcc cttttgttgg
    20461 acgtttaaaa gatgatcagt atgtttatca agggaaaaca tatcaaatgg ggcaacacgg
    20521 atttgctcgg gatatggatt ttgaagtcat tgaacatgga caagaaatgg ctagtttcat
    20581 cttaaggagt acgccggaga cattagaaaa gtatccattt gattttgaat tagtgatcag
    20641 ctatgaatta ggaggagacg gcatcacggt tcattatcaa gtggcaaata ccggtgaaaa
    20701 agaaatgttc ttttctatcg gtggacatcc tgctttcaat gtacctcttg aagaaggatt
    20761 gtcatttgaa gatttctatc tggcattttc tccattgaaa tcaagattac gcctaccatt
    20821 gaaaggccca ttcatcgata tggatcaaaa gacgttaggt cagacaaata cgaatctggc
    20881 tttgaatcgt gaactttttg agcaagatgc cttggttttt gaaacaaggg gtctaaatgc
    20941 gtacgccatt cgaagtgaaa aatcttccca tagcgtaact ttaagctata aagatatgcc
    21001 ttatgtaggg atctggtctc cttatccaaa agaagcgcct ttcgtttgta tcgaaccgtg
    21061 gtgcggaatc gctgacacgg tgaacagtag tggaaacttg atggaaaaag tagggatcaa
    21121 tcaactaggt gcccatgaaa tcttcaagac aaaatattcc atcacagtga agtaagaggt
    21181 tgtgaccgag aagttagcag ggaacaccgt ttaccaaggt gtgtaaaaaa gctatcctgt
    21241 aagtaataag ggagtgtgac atcgatttgt cacactcccg ttttatctct ttggaatgaa
    21301 aaagtatcag gattttttct tgctgctatt caaaccaaac gggattcgac tttcagtatg
    21361 gtttctgata cgcttgatat tgtctttatg ccgataaaag ataaaggaag aaactaaaaa
    21421 ggcaatgatc gtcagtaacc aattgggctt agccaaaatc acaggagcta cataaggaag
    21481 gacgatcgta gataaagtga tcaatactgc actgatcata ctgctcaaac tcaccatact
    21541 tgtacaaaat aaactaacga taaagatgat acaagaataa acaaaaaata caggattaaa
    21601 tcctaaaagc attcctgcac ttgtcgcaac agcttttcct cctttgaacc cagcaaaaat
    21661 cggaaatgta tggccaagaa tggcgcatac cccaaaccaa agagggttaa tcccagaaag
    21721 gtggaagatc atcggcaaac aagtagccaa tgtacctttt aaaatatcca taagcaggac
    21781 aacaatcccg gctgttttgc ctaatacacg aaatgtattg gtcgttccag tattaccgct
    21841 cccgtgttgt cggatatctt ttttgaaaaa caatttgcca atccagacac cggaaggaat
    21901 agaacctaaa agatatgcta aaataagtaa taagatcaat ttcatttttt accctctttc
    21961 aagttgaaaa ctatccttta ttttagcatg aataggtaac aggaacaatt gaaaaaatga
    22021 aagatacaag tataatagat ggaataaaaa gaaaagcaag gggtggcaga aatgtctgta
    22081 aaaaatccaa cacaagaaac gattttcaac tatttggaag aagcaaaaaa tattgccgtc
    22141 gtaggcttga gcaataaacc agaacgaaca agttacatgg ttgcacaggt attgcaagac
    22201 aatggatata aaatcatacc agtaaatcca ttattggctg gtgaagaaat tctaggcgag
    22261 aaagtctatg cgaatttgac agaaatcgat gatccaatcg atatcgtaga tgtatttaga
    22321 cggagtgaat ttttacctga aatagcaggt gattttttga aaactgatgc taaagtgttc
    22381 tgggcacaat tagggattga aaacaaagaa gcagctgaga tgctaagaaa agcaggacgt
    22441 acagatatcg taatggatcg ttgtattaag attgaacttg gtaagttgaa agaaaaccaa
    22501 aaataaaaat gaccacaaag aagagatgat cgatagaggg gaatgtcgaa aaagcggtta
    22561 ggagataatt aaaattgatt tctcgtctac cctctttttt ttcacgacct tttctagtat
    22621 gataatcgtg gtgcggtttt gccgcaaagt attattgaag gagcgtttgc cttggcaaaa
    22681 aaagtaaaca acgaatacaa tgactcgtcc atccaagtat tggaaggatt agaggcagtt
    22741 agaaaaagac cagggatgta cattggatcc actgacagcc gaggcttgca tcatttagtc
    22801 tatgaaatcg tcgataatgc agttgacgaa gcattatcag gttatggcag tgagattgat
    22861 gtgaccatcc atgaagacaa tagtataact gttgcagata gcggacgtgg gatgcctgta
    22921 ggaatgcatg cttccggaat accaacagtt gaagtgattt ttactgtgct gcatgctggc
    22981 ggaaagtttg gtcaaggagg gtacaaaact tctggtggac tgcacggtgt aggagccagt
    23041 gtggtaaatg ccttatcgaa atggctaatt gtaacgatcg ttagagatgg cgtagagtat
    23101 cagcagaaat ttaaaaacgg cggtaaacct gacggtactt tgaaaaagat aggaaaaaca
    23161 aaaaaagcaa acgggacgac tgtccatttt ttaccagatg atacaatatt cagtacaacg
    23221 aaattttcat atgaaatttt agccgaacgg ttaagagaat ctgcctttct tctaaaaggc
    23281 gttaaaatct ctctttctga tcttcgtgga gaagaaccag tcaaggaaat ttttcattac
    23341 gaagaaggaa tcaaagaatt tgttgattat ctgaatgaag aaaaagatac attgacacca
    23401 gttgtttatt tttcaggaga aaaagaaggt atcgaagtag aggtcgctta tcaatacaat
    23461 gacggttatt cagaaaatgt tttatctttt gtcaataacg ttcggacaaa agatggggga
    23521 acccatgaag ctggtatgaa agcggcgatg acgaaatctt ataatgaata tgcgagaaaa
    23581 gttggtttgc tgaaagaacg ggacaagaat ctggaaggta gtgatttcag agaagggtta
    23641 gctgctgtac tttctatccg tgtacctgaa aatcttctgc aatttgaagg gcaaacaaaa
    23701 gaaaaacttg gtacgccagt tgctcgtacc gtagtagata acgtcatcag tgaacaaatg
    23761 ggcttttatt tgcaggaaaa tagcgaaatg agccagatgt tggtccgtaa agcaatcaaa
    23821 gccagagaag cgagagaagc agcacgtaaa gccagagaag aaagccgaaa tgggaagaag
    23881 cgtaaaaaag gcgaatctct tctttcagga aaattgacac ccgcacaatc ccgaaaccct
    23941 aagaaaaatg aattgtattt agtcgaaggg gattctgctg gcggatctgc taaacaagga
    24001 agagacagaa agttccaagc aattctacct ttacgaggaa aagtcatcaa taccgaaaaa
    24061 gctaaaatgc aagatatctt gaaaaatgaa gaaatcaata cgatgatcta tacgattggt
    24121 gcaggagtcg gtcctgagtt ttctatcgaa gactgtaatt acgacaaagt aatcatcatg
    24181 accgatgcgg atacagacgg cgcccatatt caagtattac tgcttacttt cttttatcgt
    24241 tatatgaaac cgctgattga agctgggaaa gtatatatcg cgttaccgcc tttatataaa
    24301 gtatccaaag gacaaggaaa gaagcaagtc attgaatatg cgtggacaga tgacgaattg
    24361 gcagcaatga ttaaaaaagt cggcaaagga tatatgctgc agcgatataa aggacttgga
    24421 gagatgaatg cggaacaatt gtgggaaaca acgatggatc cgacttcccg tacgttgatc
    24481 cgcgtccgta tcgatgatgc tgcgcaagct gaacgtcgag taaccacact gatgggtgac
    24541 aaagtagaac ctcgaagaaa atggatcgaa aatcacgtgc aatttacgct cgaagaagat
    24601 ggcagtatct tagataaaaa agaggatact gaaatctctc cttcagtttc taatgattta
    24661 ttagatgaag aacgagcaga taaaaatgaa aacaatcagt tatttgaagt agaataggag
    24721 gtcacctgat ggaaaatcga caagaagtac aagaattaac tttagaagaa gtgatgggtg
    24781 accgcttcgg acgttattcc aaatatatta tccaagaacg tgcactgcct gatatccgtg
    24841 atggattgaa gccagtgcaa cgccggatat tatttgccat gaacaaagat ggcaatacgt
    24901 atgacaaagg attccgtaaa tcagcaaaat ctgtcggaaa tatcatgggg aattatcatc
    24961 ctcatggtga catcagtatt tatgaagcga tggtccgcat gagccaagac tggaagttgc
    25021 gggaagtact gatcgaaatg cacgggaata acgggagtat ggacggtgat ccacctgctg
    25081 cgatgcgtta tactgaagcc agattatcca agttaagtgg agagatgcta gcagatatcg
    25141 agaaagaaac agtcgatctc gtgtggaatt ttgatgatac agaaaaagaa ccaacagtcc
    25201 ttccagctcg atacccaaac cttctcgtca atggatcgac aggtatatct gctggatatg
    25261 caacagaaat cccgactcat aacttagctg aagtgatcga tggcacaatc tatatgatcg
    25321 atcatccaca agcgagcctt gaaaaattga tggaatatat tcctggacct gactttccaa
    25381 caggagggat cttgcaagga aaagatgaaa tcaaaaaagc ttatgaaaca ggacgaggaa
    25441 aagttattct tcgttcgaaa acaaagatag aggcaatgaa gggtaataaa cagcaaatcg
    25501 tgatcacaga aatcccatat gaagtaaata aagcgacctt ggtgaaaaaa atggatgaga
    25561 tccgtttgaa taagaaaatc gatggaatag ctgaagtacg agatgaaagt gatagaacag
    25621 gtctgcaaat tgtcgtcgaa ttgaaaaaag acgccaatgc tcaaggaatt ttgaattatt
    25681 tgttcaaaaa cactgagcta cagatcaatt acaatttcaa tatggtcgct atcgaccata
    25741 tgactccgca tcaagttgga ctgaaagaca ttttaagaag ttatatcgag catcgaaagc
    25801 aagtcataac aaaacgtagt caattcgatt tagccaaagc tcaaaaaaga cagcatatcg
    25861 tagaaggatt gatgaaagct ttatcgattc tggatgaagt aatcgcaacg attcgtgaaa
    25921 gtaaagacaa aaaagacgcc aaaaagaatt tagtcgacgt ctttcaattt acagaagaac
    25981 aagcagaagc aattgtcaca ttgcaattat accgtttgac gaacacggat atcacagagc
    26041 ttcaaaagga atccgaaagc ttgattgcgc aaatcacaga gttgaataaa atcctttcaa
    26101 atgacaaaga gcttttctct gttatgaaaa aagaattgcg agaagtgaaa aaaaattatt
    26161 cttccgctcg tctaacgaca atcgaagacg aaatcgaaga aatcaaaatt gatacgcaag
    26221 tcttagtggc acaagaagac gtaattgtca gtgtgacgcg agaaggctat gtgaagcgaa
    26281 caagccttcg ttcttatagt gcttccaaac cagaagaaat cgggatgcga gaaggcgact
    26341 acttgctata ctcaggtgaa ttgagtacat tggaccatgt tcttctgatc acaaataaag
    26401 caaatgtgat ctaccgccct gtccatgaac tacccgattt gaaatggaaa gatgctggtg
    26461 agcacatttc tcaaacaatc tccaatctgt ctgtagacga atcgatttta gctgttttcc
    26521 catatcaaaa aattcaagca gagaaaacat ttgtattcat cagcaagaat ggattgatca
    26581 agcaaacacg aatgacagag ttcgaaccat ggcgcaccta taagagccgt ccgctcagct
    26641 gtatgaagct aaaggcggaa gaagatgaac tgattgctgt gtatctgaaa gaagatcagc
    26701 caggcttaga tgttttctta gtaacgcatc aaggaatggg tttgcgctat cctttaacag
    26761 atgttcccgt tgttggagca aaggcagctg gagtgaagtc tatgaatttg aaagatggag
    26821 ataggattat caacgggctg cttgtgttag cagaaggtga tacgccgatc gtaatcgtta
    26881 ctcagcgagg agctgttaaa cgcatgcttg ctcaagaaat cagccaaact tcacgtgcga
    26941 agcggggagt gacggtcatg cgtgagctga aaaaaaatcc gcaccgtatt atctatatgt
    27001 cggaaggaag atcaaaagaa ataaccttga tgaatcaaaa agggcagcag ttaacgattg
    27061 acccaactga tttccctata ggtgatcgaa catcgaatgg atcatttgcg atggatgaga
    27121 aaaaaggtgg agaagtcatc gaagttatcg atgctcctga gattttcatc gagtcgtaac
    27181 tgttgtagta ttttaatgaa gagacgccat ctgtattaat acaggtggcg tctctttttc
    27241 attcattttt aataaatcct tatacgtttg caaaaaaaga tgaaagtgtt cacaatgctt
    27301 aaatgttggt atgtcaacgt tgatttaaat atatgtaaat aaaacataaa aattgttaaa
    27361 taaaaaacaa gaaaaccctt gcaaagatag ggtgctgtgt tatactaatc acgtaaaaac
    27421 tgttacataa aacagaaatt taggaggtcc atacaatgga gcaatggaat ggatttaaag
    27481 gtaccaaatg gaaagaaggc gtagacacta gagactttat tcaaaataac tacactgaat
    27541 atcgcggtga tgacagcttt ttggaaggcc caacagaagc aacagaaaaa ttatgggatg
    27601 aattgcaaaa gttaaatgat atccaattcg agcgtaacgg tgtctatgat atggacacag
    27661 aaatcgtatc aactattact tcacacgatg caggctattt ggataaagat ttggaaaaaa
    27721 tcgtcggtct acagacagac aaacctttga aacaagcatt catgccgttc ggcggaatca
    27781 atatggctaa caatgcgcta aaaagcaatg gctatacacc aaacgatgaa ttgacacata
    27841 ttttcactga ctggcgcaaa acacataatc aaggtgtatt tgacgcttat actgctgaaa
    27901 tgcgtgctgc tcgtaaaaac aaaatcatta cagggcttcc agacgcttac ggtcgcggac
    27961 gtatcatcgg tgactatcgt cgtgtggcat tgtatggaat cgactatttg atggaacaaa
    28021 agaaaaaaga tcatgatatg actggttatc atacaatgag tgaagaagtg attcgtttac
    28081 gtgaagaaat cactgaacaa tatcgtgcat tagcgcaaat gaaagaaatg gctgcgaaat
    28141 atggctatga tatttctaaa ccagctgcaa atgcaaaaga agctgtccaa tggctatact
    28201 tcggatattt agcagcaatc aaatcacaaa atggtgctgc gatgtcaatc ggacgtatct
    28261 cagcattcct tgatatctat atccaacgtg atttggataa tggagtaatc acagaaaaag
    28321 aagcacaaga attgatcgac cacttagtaa tgaaattacg tatggtcaaa ttcgcgcgta
    28381 cacctgaata taaccaatta ttctcaggat tccctatctg ggctacttta tccattgcag
    28441 gtatgggatt agatggtcgt tcattagtta caaagaatga tttccgtctg cttcatacat
    28501 taacaaatat gggaccttca ccagaaccaa acttgactgt tttgtattct gaacaccttc
    28561 cagaaggatt cagaacatat gctagcagaa tcgctatcga aagttcttcg attcaattcg
    28621 aaaatgataa tcagcttcgt gaacaatggg gttctgatga ttgtgcgatt gcttgctgtg
    28681 tatcaggaac agttgtcgga aaagacatgc aattctttgg cgctcgtgct aacttggcta
    28741 aaacactatt atatgccatc aatggcggag tcgatgaagt gactggcgcc caagttggac
    28801 cagaatatcg tccaatcaca tcagaaatac tagactttga agaagtgaaa cataacttca
    28861 tgaatatgat ggattggtta gcagaactat atgtcaatac gttgaacatc attcattata
    28921 tgcatgataa atattcttat gaagcagctc agctggcttt gatggattct gacttgcaac
    28981 gcacatttgc tacaggtatt gctgggatct ctcatgcggc agacagcttg tcagcgatca
    29041 aatatgcaaa agtaaaagca atccgtaatg aaaaaggaat agcagttgat tttgaagtgg
    29101 atggcgactt ccctaaatac ggaaatgatg atgaccgtgc agatgaatta gtttcatgga
    29161 tcttggaata cttcatggga caaatcaaac gtcataaaac ttaccgcaac tcaaaaccaa
    29221 caacttcatt attgacgatt acttcaaatg ttgtttacgg taaaaataca ggaaatactc
    29281 ctgatggccg tgaagctgga aaaccacttg cgccaggagc aaatccaagt tacaatgcgg
    29341 aacaaagtgg gttattagct agcctgaact caacagctaa aatgaattac ggctgtgcat
    29401 tggatggaat ttcaaatacc caaacaatca atccttcagg tcttggaaaa gaacaagata
    29461 caagaatcga aaacttgcgt aatgtcttag atggatactt ctcaaaagga gcttatcatt
    29521 tgaatgtcaa tgtattcagt aatgatttat tgcgtgatgc aatggaaaat ccaatgaaat
    29581 atccgaactt gacgatccgg gtatctggtt atgcagttaa attccgtgac ttgacaagag
    29641 aacaacaatt agatgtcttg atgagaactt cacacgacag aatgtaaaaa gttcgtgaaa
    29701 aaagtttttc gacttagcgt cgaaaggacg ttgaatagcc atcgcatcaa aatcgaataa
    29761 tcagtatgct tatttgaaag aaacgattta caggggcagg acttgtatgt tccgtccctg
    29821 taaattttac acataacgaa gcggcaattg cgaaaaattt tgttcagaaa gcttcgcttc
    29881 gacagctaac ttgtacttaa aatgaaatca aagaaaagat gtgaacttaa atggaagaaa
    29941 aaacaatcgg ctatgttcat tctatcgaaa ctttcggttc agtagacgga cctggactgc
    30001 gctttgtcgt gtttatgcaa ggatgccgca tgcgttgcca attttgtcac aatccagata
    30061 cttggaatat tggcggaggg aaagaataca cagcagatga acttttagat aaagcagaac
    30121 gcttcagacc ttactgggga gataaaggtg gtattactgt tagtggtgga gaacctctat
    30181 tgcagatcga ttttttgatc gaactattta aaaaagcaaa agagcggaag atgcatacta
    30241 cattggatac atgcggaaaa ccttttacgt atgaagaacc tttcttttca cgttttcaag
    30301 aattgatgaa gtatacagat ttactgttat ttgacatcaa acacatcgat aatgaagagc
    30361 ataaaaaact aacgcattgg gacaatgaaa atattttaga aatggctcaa tatttgtcaa
    30421 aaatcaacaa accggtttgg attcgccatg tactagttcc tgagcgaagt gattatgatg
    30481 aatatttgat ccgactagac aactttattc aaaccttgtc aaatgtagac agagtagaga
    30541 ttcttcctta tcatacgatg ggaaaataca agtgggaaac acttggtttg aaatatccat
    30601 tagaagggat cgaaccacct acaaaagaac gtgtcgaaaa tgctaaacga ttgcttcata
    30661 cagaagagta tacaaaatat ttaacgcgat aggtcgcatc ggaaagaaga taagtattgc
    30721 ttctttccgt ttttttgcta ttattaaata ggagaaatca caaaggagcg aatgacatgt
    30781 caaaaatttt agtttttggt caccaaaatc cagatacaga tgcaatcggg gcagccattg
    30841 cgttttcaca cttgcaaaat gaattaggta aagaaacaga agcagttgct ctaggtacac
    30901 ctaatgaaga aacacaatac gcattagacc actttcagct atctgcacca cgagtagtag
    30961 agtcagctaa agcagaaaat gcagatgtca tgcttgtgga tcataatgaa ttccaacaaa
    31021 gtatttcaga tatcagcgaa gtaaatattt tagctgtagt agaccaccat cgtattgcaa
    31081 actttgaaac agctaatcca ttgtactatc gtgcagaacc agttggatgt acaagtacga
    31141 ttattttaaa actatataaa gaaaaccaag taacaattcc taaagaaatc gcaggaatca
    31201 tgctctcagc tatcatttca gatacattgt tgttcaaatc accaacatgt acgcctgaag
    31261 atgaagcagc tgcaaaagaa cttgcagcaa tcgctgaagt cgatctaaac acttatggac
    31321 ttgaaatgtt gaaagcagga acaaatctaa gtgataaatc agctgcggtt cttcttgatt
    31381 tggatgcaaa aagtttccca atgggcagtt ccaacgttcg tatcgctcaa gtcaatacag
    31441 tggaccttaa tgaagtgatg gatcgacaag cagagctaga agcagcaatg gaagatgaaa
    31501 tgaaagcaaa tggctacgat ctgttcgtat tagttgtcac aaacatctta gacagtgatt
    31561 cagaattact tgttgctggt aaaggaaaag aaaaagtaga agaagctttc caaacaactt
    31621 tgaccaacaa ccgtgctttg cttaaaggtg tcgtttcacg taaaaaacaa gtcgtacctc
    31681 aattgacggc tgtatttagc taatcaaatg gtagtatgaa gcattcaaaa tgaatggata
    31741 agcgattgat ataaaaagaa acatagaaca tactaaaggc cgaagaaatg gaggagaata
    31801 acagtcaatt atgactcttg ttcttcctcc tttttacgta aaagcctttt aagtaaaata
    31861 aaacggatag aaaagagaac atatatgaac tattattcaa aaaataagga aaacttttat
    31921 caaaaactga ccgatgatcc gcttttttct tcattgaccg attatctata tgaacatcga
    31981 gaacaagaaa cgattctaag agaattgaaa aaagaatttt cgcaaaataa attttcccat
    32041 tttcttgatt tattgatcga tgcaggatta atcaagaggg aagaacgaag atatcatttg
    32101 aactttccaa tatttgatcc taaagattac ttgcaacaag ccacttcggc tgctgaaacc
    32161 atagctgaac aattgaagag actctctgtt gatgaacaaa aattagcgat gggagaagtt
    32221 atttgggcgt attgtttcga agatgaacgg aaagaagcgt atttttatgg agtaagaaac
    32281 agccgagaga cagaattgct gaggacaact gcaggaaatg agaagtaccg ttttatcaca
    32341 ctttcctcta tagaacactt cccgcttact ttggctaatt acttttttgt acagaagaat
    32401 cagcttcctg taacgaaggc attcaaagaa ttagccgaac ttattggaga cgtaaatgaa
    32461 tcgtactttt ttgatcaaat agaagttatc gttgacagga tacgtaagaa taaatataaa
    32521 aatcgccggc ctagtatttt ccatcagtct ctacttgtga cgaatactat aaaggaagaa
    32581 gaatcattca cattagaatt acctattgtc gaaaaaaaca attttgagat tgaattacca
    32641 acattggatc cttcattgac aatggaggaa atggcctttc ttaaacgaca aatcttttct
    32701 gagctgagca agaaatttat accgcatgca ttttcttata tcaaagaata tggaacagtt
    32761 ttagtgagta aaacctaaga gagcagtaag caaaaattat actgactctt gcaggaggag
    32821 cctttctctt cctttatggt taaaagaatt atttgagtat tttgcctatc taccacctat
    32881 tagattcact acatggtctg gaaacaaagg aatgcaaaaa aatggtgaga aatgtggaat
    32941 gacaaaggaa gtagtgataa gaaaagttag atttttaaat aatcaatatt atgattcagt
    33001 caagtatggc attttatggg aagaactagc tgattagatt aggaagtcca tttgataatc
    33061 cagaatcact aagaaacgaa tggcgagtca ttaataattt atccaatctt gggttattct
    33121 tttagattga agtgtttaaa actaaaaaag catttatgaa gtcgtaatag tcaggcttca
    33181 aaatgctttt tattttgttt gttttttctt tgccacataa atacacctag actgacgatg
    33241 aagagcatat ccaacagatt tcctgcgact tggatcgtcg aagggttggt cagtggcatt
    33301 aaagcaagga caaaaaagga aaacaagctg taaataaaac aggtgacagg aactaatcga
    33361 aagaagagcg gacatttcaa ttggctaaaa atgagtgaaa aaataaaata agtaaaaatg
    33421 gataaaatca cgactaaacc attcatgata aagaagaaaa gcgtattgtg attaaacgaa
    33481 taaaaaagaa ttgctaaaaa agaaaacgtt ttaaatgcaa tcaatacgct tgaagcttcg
    33541 tttaacgtac caggatgtcg atgaaaaaaa taggcaccga aataaacaga aaacagtaag
    33601 ccgcaaagag aagacaaaat caaaaaaaac ggattttctt cggcttctgt gatatcagaa
    33661 tgatccaaaa gcaaccagcc taatgcacta gaaggcgcat gtgaggtctt aaggtatgaa
    33721 taacatagaa aaaataagat cgtcaaagtt gcaaaaaaag agcctaaatg taaaaaatta
    33781 gtggttctac gttgaatttc cataatatct ccaaattaaa ttgataagta tataaagtat
    33841 aataacaaat tgatgagtat atgtcgatgt tttttaatca aaataataaa taaaaactgt
    33901 ctaacattta aaatttaatt tagatattag acagttttta agtttgtgag ttattttcct
    33961 tgtcgttttc tcactttttt agtccagaaa ccacctgaaa tatattttgg ctcataagaa
    34021 atgataaaag cacgctcttc gatttctttg atcaggcgat acagttcacg ttcgcttttt
    34081 ctaggagaaa gtatttcgag aactagccgc tcaccttcca gccccattgc agaagtctgt
    34141 gtcacaccat aaccgtgttc tctcagtata cccggcaaac ttttatcttc tgcgctattt
    34201 gctggcaaaa tcgttgtgac catgatgtat cctaatgcca atttgtcttc aatcaaaatt
    34261 ccgacactga tacctactgc gtaacctaac gcatagacaa ataaattcag tggattatct
    34321 aaacgatcta aaacaaggga taagccgacg atataaatcg tgatctcgac catactgaca
    34381 agtggagcaa tcagtcgata gcctttcatt gtcaacatga aacgtaacgt gttcagagtg
    34441 atataagcaa aattaatgag gaagatcatc cccaacattt ttggatcaat catttgtaaa
    34501 actcctttca tttatataac aacaatatca aaaaaaatcg taagtgaaga aaaattgaaa
    34561 ctttatacat acaaaaaaat gataaaatga ttataataga attataaaga taggttaggt
    34621 gattaaaatg aaaacggtca atcgtgtgtt ttgtgccagc cacgtgctaa gtctccttct
    34681 cagtatatac t
//
LOCUS       DACCXM010000322        27180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.guided.025, whole genome shotgun sequence.
ACCESSION   DACCXM010000322 DACCXM010000000
VERSION     DACCXM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.guided.025"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..353
                     /locus_tag="IVY61_003008"
     CDS             <1..353
                     /locus_tag="IVY61_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7972031.1"
                     /translation="FFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRL
                     ESNPQMSTEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSN
                     TLDILYKNTIFTNL"
     gene            386..1060
                     /locus_tag="IVY61_003009"
                     /pseudo
     CDS             386..1060
                     /locus_tag="IVY61_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1317..2516
                     /locus_tag="IVY61_003010"
     CDS             1317..2516
                     /locus_tag="IVY61_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7972032.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            2538..2750
                     /locus_tag="IVY61_003011"
     CDS             2538..2750
                     /locus_tag="IVY61_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7972033.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            2825..3301
                     /locus_tag="IVY61_003012"
     CDS             2825..3301
                     /locus_tag="IVY61_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP7972034.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            3298..5106
                     /locus_tag="IVY61_003013"
     CDS             3298..5106
                     /locus_tag="IVY61_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7972035.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            5183..5398
                     /locus_tag="IVY61_003014"
     CDS             5183..5398
                     /locus_tag="IVY61_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP7972036.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            5490..6353
                     /locus_tag="IVY61_003015"
     CDS             5490..6353
                     /locus_tag="IVY61_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7972037.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            6384..6926
                     /locus_tag="IVY61_003016"
     CDS             6384..6926
                     /locus_tag="IVY61_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP7972038.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            6940..7362
                     /locus_tag="IVY61_003017"
     CDS             6940..7362
                     /locus_tag="IVY61_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7972039.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            7292..9691
                     /locus_tag="IVY61_003018"
     CDS             7292..9691
                     /locus_tag="IVY61_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7972040.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            9723..11714
                     /locus_tag="IVY61_003019"
     CDS             9723..11714
                     /locus_tag="IVY61_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7972041.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            11737..11988
                     /locus_tag="IVY61_003020"
     CDS             11737..11988
                     /locus_tag="IVY61_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP7972042.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            11978..13207
                     /locus_tag="IVY61_003021"
     CDS             11978..13207
                     /locus_tag="IVY61_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP7972043.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            13204..15285
                     /gene="topB"
                     /locus_tag="IVY61_003022"
     CDS             13204..15285
                     /gene="topB"
                     /locus_tag="IVY61_003022"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7972044.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            15434..19354
                     /locus_tag="IVY61_003023"
     CDS             15434..19354
                     /locus_tag="IVY61_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7972045.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            19355..20299
                     /locus_tag="IVY61_003024"
     CDS             19355..20299
                     /locus_tag="IVY61_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7972046.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            20303..20506
                     /locus_tag="IVY61_003025"
     CDS             20303..20506
                     /locus_tag="IVY61_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7972047.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(20806..21042)
                     /locus_tag="IVY61_003026"
     CDS             complement(20806..21042)
                     /locus_tag="IVY61_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7972048.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(21225..21596)
                     /locus_tag="IVY61_003027"
     CDS             complement(21225..21596)
                     /locus_tag="IVY61_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7972049.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(21593..22921)
                     /locus_tag="IVY61_003028"
     CDS             complement(21593..22921)
                     /locus_tag="IVY61_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7972050.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(22882..23211)
                     /locus_tag="IVY61_003029"
     CDS             complement(22882..23211)
                     /locus_tag="IVY61_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7972051.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(23469..23839)
                     /locus_tag="IVY61_003030"
                     /pseudo
     CDS             complement(23469..23839)
                     /locus_tag="IVY61_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            23984..24169
                     /locus_tag="IVY61_003031"
     CDS             23984..24169
                     /locus_tag="IVY61_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7972052.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            25112..25771
                     /locus_tag="IVY61_003032"
     CDS             25112..25771
                     /locus_tag="IVY61_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7972053.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            25774..27117
                     /gene="vanS"
                     /locus_tag="IVY61_003033"
     CDS             25774..27117
                     /gene="vanS"
                     /locus_tag="IVY61_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7972054.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT         6857 a         7093 c         7347 g         5883 t
ORIGIN      
        1 tgttttttct taatacacct aattcaatta gtttgagcaa tgaaatcact aatgaacaga
       61 aatttacgta ttcgccatgg ctatatagcg aactgacgac agcttcaata gtggaaaaaa
      121 agaatccacg attggaaagt aatcctcaaa tgagcaccga ggatgttaga agcattataa
      181 agcactattc ttcgcaagaa aatctgcaaa taaaatattc tttaaattta agtgaattaa
      241 cttatttacg aaaagctgat ttgaaaaaat gggctttaga tgctgtggaa tgcactaaag
      301 gaagtaatac attagatatt ttatataaaa acaccatttt cactaatctc taatcaattt
      361 actgaatcga aaggaaccgg gtagaatgga taagaaagtt ttaataaaga ggttctctga
      421 agctattgaa aaaggaaatg cagcaatatt tgcaggtgca ggattatcga tgtcacaagg
      481 atatgtaagt tggccggaac tacttaatga tcctgcaaca gaaatcgggt tggattctaa
      541 aaaagaaaca gacttagtta ctctggctca atattataaa aatgaaaatg gtggatcaag
      601 aggaattttg aatcagatac ttatggataa ttttggtgaa gaattagaaa tatctgaaaa
      661 tcatcgaata ctagcaagtt tacctattga aacttattgg acaactaatt atgatcatct
      721 aatagaaaaa tcaatccgag aagcttataa aaaccctcag gttaagaaaa attatactca
      781 gttagcaaca actaatccaa atgtggatac aattgtttat aaaatgcatg gtgatataga
      841 tgatgtcagt tcgacagtaa taacaagaga tgattatgaa aaatatgatg attcgtacgc
      901 gttatttaaa gaaactttaa aaggagattt gttaacaaaa acttttctct ttttaggatt
      961 tagttttaca gaccctaatt tagaaagaat tttatcagat ataagatggg ttttgagaga
     1021 aaaccaaaga cctcattatt gtataatgag aaaaatatta tagaaaaaat tttaggttat
     1081 cgctgaaagc cccggaaata cgggcatttc gcaggatatg agaactcaat ttactaccaa
     1141 tttactactt tcggattgag ccgcatatat gctgaaccat actgctaata cccgccaccc
     1201 caaatacggc acatcataaa ttgaatacga caccgtaaac gcggcgttcc tctttcccgt
     1261 catgggagaa caggacgccg cttttttatg cccaaataga gaaaggaggc gacgccatgc
     1321 tatttacttc cggccctgac cgggcgtttg aacggctgat gcaggaaaag cccggtttcg
     1381 atcactacga cggcggcggg gcggacgcgc cggaggattg cggcacctgc cgattttacc
     1441 gcccggattg gaaatatcaa ttctgccagt ttgcagagtg tccctatcag ccgggcaagc
     1501 tgaccgacta tgcagcggtc aaattcacag tgaaaggagc gaacaacgct atggcagttt
     1561 tccgtgtgga aaagaacaaa ggctatacgg tgatgtcaaa tcaccacctg cggaataaga
     1621 acctgacctt aaaggccaag ggcctgctgt cgcagatgtt gtccttgccg gaggattggg
     1681 actataccct aaaaggactg tcctatatca accgggaaag catcgacgcg atcaggacag
     1741 cggtatggga gcttgaaaaa gctggatata tccggcgtga gcagggccgt gacgcaaaag
     1801 gcaaaatggc cgatatggtc tataccatct acgaacagcc ggtattggaa aacccggtat
     1861 tggaaaatcc aacatcggat aacccggtgt tggaaaatcc gacatcggat aatccggcgt
     1921 cggaaaatcc aacgcaatta aataaagaag tacaaagaac tgacttatca aaaaaagaaa
     1981 aaataattac agatggacaa agtacccatt ccattcctat cccatccccg acccctgccc
     2041 ctatggaggg cgaggcagcg gctacgccgc cggaacggaa aaggacagga aaggatgagg
     2101 ctgtacggat ttatcgggaa atcattttgg agaacatcga gtatgcctat ctcattcagg
     2161 acaggagcat tgaccgtgaa cagcttgacg aaatcgtaga cctgatgctg gaaaccgtct
     2221 gcaccgcccg aaagacaatc cgtgtcgccg gggacgacta ccctgccgag ctggtgaaat
     2281 ccaagtacat gaagctgacc ggcgaacata tccgctttgt ccttgactgt ctgcgggaga
     2341 acacaacgaa agtccgcaat atcaaacaat acctgcgggc tatgctgttc aacgccccca
     2401 gcaccatttc caactactac acggcccttg tcgcgcacga catggcacag cccgattggg
     2461 gaaagcctaa atccggcctg ccggactatt cctgcggcga gggcgaaagc ctgtaacgag
     2521 aaaaggagga cgaccctatg aagcagggcg cattgatttt tgacgagcga acagaccgtt
     2581 acgacatccg ctttgatctg gccgactatt acggcggcct gcattgcggg gagtgtttcg
     2641 acgtgatgat cggcggcaaa tggaggccca cccgcattga aatggccgaa aactggtatc
     2701 ttgtcggcat ccgggccgac gacctttccg gcctgcgggt gcggatttga cctatatcta
     2761 cctgcggcgt ctggtggttc cgccttagac gccgcttttg actttctgac aaggagggat
     2821 gcgattgcag gaagaagtta ctcaaaagac cattgccctg tccatgaaaa cgggcaagct
     2881 cacggctcaa gtgctgcaag cggcgctgaa aaagtacctg caacaccggg ccaagggcaa
     2941 aaacacgctg caccacggcc agcagagcct aaagcagctt aaaaagcatg gggctgatct
     3001 ctccaacatt gagatcaccg aggccaacat tggagcgttc aagccatgcg ccaagaaata
     3061 cggcgtggat tttaccttac gaaaagataa gaccacccag ccgccccact acgttgtgat
     3121 tttcaaggcc aaggatgcag ataaccttga tctggcgttt cgggagttta ccgccaagac
     3181 gctttccaag gagcagcggc cctctatccg caaggttttg tccgctgtga agcaaaaggc
     3241 ggcggcccag accaagcagc gggccaagga gaaaatcaag gaaagggggc tggaactgtg
     3301 aagcctgacg taaagaaatt gctgatcctg aacctcccgt atctgatttt tgtgtacctg
     3361 tttgcgaaat gcggggaggc gtaccggctg gccgctggtg ctgacctgtc ggggaagctc
     3421 ctgcattttg cggatggttt tgcggcggct tttgccaacc cgctgccaag cctgcacctg
     3481 tttgacctgt gtatcggtat cgccggtgcc gtgatcgtgc ggctggtggt gtactgcaag
     3541 agcaagaacg tgaagaaata ccgcaaaggc atggagtatg gcagcgcccg ctgggggacg
     3601 gccaaagaca tagcgcctta catcgacccc aagtttgaaa ataatatcct gctcacccaa
     3661 acggaacggc ttaccatgac cggcaggccc aaagacccca agacagcgcg gaataaaaac
     3721 gtgctggtga tcggtggcag cggttcaggg aaaacgagat ttttcgtgaa acccaacctg
     3781 atgcaatgct ttccgaccac ggactatccg acttcgtttg tggtgacaga tccgaaaggc
     3841 actttggttc tggaaaccgg caggatgttc cagcaggcgg gctaccgcat taaaatcctg
     3901 aataccatta acttttccaa gtccatgaag tacaacccct ttgtctacat ccattcggaa
     3961 aaggacgtgc taaagctggt aaataccctg atttccaaca ccaagggcga gggggaaaag
     4021 tcagcggagg atttttgggt gaagtcggaa cggttgtttt attcggccct gatcggctat
     4081 atctggtacg aggccccagc ggaggaaatg aacttctcaa cgctgctgga aatgataaac
     4141 gccagtgaag cccgcgagga cgacccggag tttcaaagcc ccgtcgatct catgtttgag
     4201 cgtttggagc agaaagaccc cgaccatttt gcggtgcgcc agtacaagaa attcctgctg
     4261 tccgcaggca agacccgttc ttccatcctg atttcatgcg gtgccagact tgcgcccttt
     4321 gacatccggg aggtacgcga gctgatggag gacgacgaaa tggagctgga caccatcggg
     4381 gacgaaaaga cggtgctgta tctcattatg agcgatacgg acacgacctt taatttcatt
     4441 ctggctatgc tccaaagcca gctaatcaac ctgctttgtg accgggctga tgatgtgtac
     4501 ggcgggcggc tgcctgtcca tgtacgcctg attttggatg aatttgccaa cattggccaa
     4561 atacccaatt tcgataaact cattgccacc attcgaagcc gtgaaatatc ggcctctatt
     4621 attttgcaga gccagtcgca gctaaaagcg atttacaagg acgccgccga gatcatttcg
     4681 gataactgcg atagtgtgct tttccttagt gggcgcggca aaaatgccaa ggagatttcc
     4741 gatgcgctgg ggaaagaaac gattgacagt ttcaacacca gcgagaaccg gggaagccaa
     4801 acgtcccacg gattgaacta tcaaaaatta ggaaaggagt tgatgtccca agacgaaatt
     4861 gccgtgatgg acggcggcaa gtgtatcttg cagctacggg gtgtgcgccc gtttttatcg
     4921 gagaaattcg atattaccaa gcatccccgg tacaaatacc ttgccgacgc cgacaagaag
     4981 aatacctttg acgtggacag atttttgact atcacgcgcc ggaaacggca gcaagtggta
     5041 acgcaggatg aagtattcga cctgtatgaa atcgacctgt cggacgagga cgccgccgct
     5101 gaataaacag cggctttttt cattccaaca acaaaatcaa aaaatcatta tgagccggaa
     5161 accggcagaa agaggtaaat ttatggcatt ttttaattcc gcagttacta ctttgcagac
     5221 tatcgtgatc ggactgggcg gcgctctgtg cgtatggggc ggcatcaatc tgttggaggg
     5281 atacgggcag gacaatccgg gggcaaaatc gcagggtgtc aagcagcttg tcgctggcgg
     5341 cggcgtggcc ctgatcggtg tcactctggt tcccctgctg tccgggctgc tgggctgatc
     5401 ctggcctttg tccctgtcca aacccttaac taatcgcctc cgcgtcctcc cggccagacc
     5461 gggagggcgc gggaaaggag gtgctacccg tgattagtga aatcattgaa gaatggataa
     5521 aaggcatcct catggacggt atcacgggca acctgtccgg gctgttcgat acggtaaatg
     5581 ccaaagtcgg agaaatcgcc gccgacgtgg gacgaacccc gcaggcatgg aacagcggta
     5641 ttttcaatat gctgcacagc ctgtcggaaa ccgtgatcgt ccccatagcc ggggccatac
     5701 tcgcccttgt catgtgctat gagcttatcc agatgataac ggaaaagaac aatatgcacg
     5761 actttgacag ctccatgttc ttccgctgga tattcaaatc cgcgtttgca atcctgatcg
     5821 taacgaacac atggaacatc gtcatgggcg tattcgacgc cacccaaagc gtcgtcaatc
     5881 aaagcgccgg tgtgattatc gggcagacat ccattgattt taacaccctg ataccagacc
     5941 ttgaaagcca attggaggct atgagcgtgg gcgctctttt ggggctgtgg ttccaaaccc
     6001 ttgttgtggg cctcaccatg aatatccttt ccatctgcat tttccttgtg acctatggcc
     6061 gcatggtgga aatctatgtt gtcacgtcgc tggggcctat cccccttgcc acaatgggca
     6121 acgcggaatg gcgcggcatg ggccaagggt atctgaaatc cctgctggcg ctgggcttcc
     6181 aagccttttt aatcatggtt gtggtgggta tctacgcggt gctgatacag aatatcggtt
     6241 ccgcttccga catatccggc gcaatctggg gctgtatggg ctatacggtg ctgctgtgct
     6301 tctgcctgtt caagaccggc agtatatcaa aggccgtatt cggggcgcat tgatcccaaa
     6361 atccaagcat acacaggagg aacttgttta tttcaacgta caacatcatt tacgctgacc
     6421 cgccgtggcg gtatgactgc aaaaacgtac agggcgcagc ggaaaatcac tattccacca
     6481 tgagcattga cgagctgtgc gccctgccgg tggaacggct ggcgtccaaa gactgcctgc
     6541 tgtttttgtg ggccacattc cccatgctgc cggaagctct gcggttaatc aaagcgtggg
     6601 gcttttcatt caagaccgtg gcttttgtat ggctgaaaca gaaccgtaaa agccttacat
     6661 ggttttacgg gctgggccgc tggacaaggg gaaacgcgga aatctgcctg ctggcaacac
     6721 acggcaagcc gaagcgtcgt tctgcctctg tccatcagtt tattatcagc ccgattgaac
     6781 aacacagcaa aaagccggat gtgacccgtg aaaaaatcgt agagctggcg ggcgatttgc
     6841 cccgcgtgga gctgtttgcc agacagaaaa cccccggctg ggacgtgtgg gggaacgagg
     6901 tggaatgtga ccttatcctg acgtgaaagg aggtgccaaa tggcttatgt aaccgtcccc
     6961 aaggacttaa cccatgtgaa aagcaaggtt gtgtttgggc ttaccaaacg gcaactgatc
     7021 tgtttttccg gggcgctggt gatcggcgtc cccttatatt ttctcacccg tgactatctt
     7081 accaacagcg cggcggccct gctcatggta tttgccatgc tgccgggctt tttgcttgcc
     7141 atgtacgagc gtcacggcca gccccttgaa gtggtgatta tgcagatcat ccagtgctgc
     7201 ttcatccagc caaaggagcg tccctatcag accgacaaca cctacaccgc ccttgtgcgg
     7261 caataccaga tggaacagga ggtaaaggcc attgtccaaa aaggaaatcc caagagaaac
     7321 cgaaaaaaca aagctgaccc gcgcccagcg aaaagaaatt gacgcggtaa tccgtaaata
     7381 taagggcgac ggcaggcccc acacggcgca gcagagcatc ccctacgagg tcatgtaccc
     7441 ggacggcgtg tgccgggtat cgcccggcgt cttttccaag tgcattgagt ttgcggacat
     7501 cagctatcag cttgcccagc cggacacgca gacggctatc tttgaaaaac tgtgcgacct
     7561 gtataactat gtggacgcct ccattcatat ccagttttct ttcctcaatc ggaaagttga
     7621 cccggtgcag tacgccaaga gctttgagat cgcgccgcag ggggacgact tcgacgacat
     7681 ccgggccgag tacaccggca ttctgcaaaa gcagcttgca aacggcaaca acggcatggt
     7741 aaagaccaaa tacctgacat tcaccatcga ggccgaaagc gtgaaagcgg cgcgagccag
     7801 attgaagcgc atcggctttg acctgttagg ctatttcaag agcatggggg ccgtagccca
     7861 tgtgatggac ggctgggaac ggctgaacct gctgcatggc gtgtaccacc cggacggcga
     7921 gatattcaat tttgactgga agtggctggc cccgtccggc ctctccacca aggattttat
     7981 tgccccgtcc tccctgtgct tcggcaatgc aaagacattc ggcatgggcg gcaaatacgg
     8041 cgcggtaagt ttcctgcaaa tcctgtcccc ggagctgtcg gacgatatgc tggccgactt
     8101 cctcaatacg gaaagcggtg ttttagtcaa tctccatgtg caggccattg aacagaccaa
     8161 ggcgatcaag accatcaagc gcaaaattac cgaccttgac gctatgaaga ttgcggagca
     8221 gaaaaaggcc gtccgcagcg gttacgatat ggacattctt cccagcgacc ttgccaccta
     8281 cggcgaggac gccaagaagc tgctgacaaa gttgcagacc cgcaatgaac ggctgttcca
     8341 gttgacgttc cttgtcctca acgtggccga caccaagcaa aagctgaata acgacgtgtt
     8401 ccaagccgca ggcgtggccc aaaagcataa ctgtcccctt gtccggctgg actaccagca
     8461 ggaacagggc cttgcaagca gcctgccgct gggcgtcaat cagatcaaga tacagcgcag
     8521 ccttacgact tccagcgtgg ccgtgttcgt cccctttgtg acgcaggagc tttttcaggg
     8581 cggcgcggcc atgtattacg gcatcaacgc caagtcccgc aacatgatta tgctggacag
     8641 gaagcaggcc cgttgcccca atgccttgaa gctcggcacc cccggcagcg gcaaatccat
     8701 gagctgtaaa tccgagatcg tcagtgtgtt cttaacgacg cctgacgata tttttatttc
     8761 agacccggag gccgaatatt acccccttgt caaacggctc cacggacagg taatccggct
     8821 gtcgcctacc agcaaggact ttgtgaaccc gctggacatc aacctgaatt attcggagga
     8881 cgataacccg ctggccctga aatccgactt tgtgctgtcg ttctgtgagc ttgtcatggg
     8941 cggcaagaac gggctggaag ccattgaaaa gaccgtgata gaccgggctg tgcgcgtgat
     9001 ctaccggccc tatctggcag acccccgccc ggagaatatg ccgatcctgt ccgacctcca
     9061 caaagcgttg ttagatcagc atgtcccgga ggctgaccgg gtggcgcagg cccttgacct
     9121 gtatgtgtcc ggctccctga acgtatttaa ccaccgtacc aacgtggaca tcggcaaccg
     9181 gcttgtgtcc tttgacatta aggagctggg caagcagctc aaaaagctgg ggatgctcat
     9241 tgtccaagac cagatatggg ggcgcgtcac ggccaaccga agccaaggaa aggccacatg
     9301 gtattttgcc gacgagttcc atttgctttt gaaagaggaa cagacggcgg cctattctgc
     9361 ggagatttgg aagcggttta ggaaatgggg cggcattcca acaggtgcca cccagaacgt
     9421 caaagacctg ctttcctccc cggaaattga aaatatcctt gaaaacagcg acttcattac
     9481 gctgctcaat caggcgtccg gggatagaaa aatccttgcg gaacgcttga atttgtccac
     9541 cgaacagcag aagtacatcg acaattccga acccggcgag ggccttttga tctttgaaaa
     9601 cgtggtgctg ccctttacca accccatccc gcacaacacc cagctttaca agattatgac
     9661 gacccgctta aacgaggtgg cgggagtatg acccggcgac gccacaaagg aggtgaacca
     9721 ccatgaaaga gctgaaagcc aaggcgaaag tgacgcaggc catgacccgc gacgggctga
     9781 cgctggaaaa tcaggccacc ggcgaggtgg agaacatttc cagccgggag gccgaacagg
     9841 atttgtccgc gccggggccg ggcggcaccg ccgataagct gctggaacgg gccgacgcca
     9901 tccatgacgg ccataaggcc aagaaagccg cgaagcaggc cggggctgtg gccgccgagg
     9961 gggacgctgc cctgcaccgg ccatccgccc ggctccaact gtcggaggaa gaacgggccg
    10021 acccggattt acagccctat atccgaaaat cggacaaacg ggccgataag ctggataagg
    10081 cccgcgccgc catccccaag aagcgcgtcc cggtcaagga aaccgtcttt gacgaggcaa
    10141 gcggcaaagc aaagaccgtc ctgcactttg acaaacagga caaggggcct ccctccctga
    10201 aacccaatcc ggcaggccgt cctgtttccg aggtttttct attcggccac gggaaaatcc
    10261 atgaagtcga gcatgaaaac gtgggtgtgg agggcggcca taagggggag gaacttgccg
    10321 agcggcaggc cggaaaagcc atccgcagca ccatccggca ccacaggctg aaaccctact
    10381 gggacgcgga aaaggccgag cgcaggctat tgtctgccaa cgcggagtat ttctaccaga
    10441 aatctttgcg ggataacccg gagatcgcac aggcggccag caaccccatt tcccggttct
    10501 ggcagaaaca gcagatcaag cgccagtacg ccaaggccgc ccggacagcg ggccagaccg
    10561 caggcgcggc ggcacagggc gcggccaaga cagccggggc ctccacgaaa gcggccaagg
    10621 ccgcagccaa agaaagtgaa cgggcggcct cctttgtggc ccgccactgg aaaggggcgc
    10681 tggccgtcct tgtgttgggg cttttgatgc tgcttatcat cggcggctta cagtcctgca
    10741 ccgccatgtt cggcagcgcc ggaagcggca tcacggcgtc ctcttacttt tccgaggata
    10801 gcgatatgtt ggccgccgag gacgcctacg cggagctgga agccgagctg caagactatc
    10861 tggacaacta cgaggccacc catgactatg acgaatacca ttttgacctt gacgagatca
    10921 gccatgaccc ctatgtgctg atttccattc tgtcggcgct ccatgacggg atatttacca
    10981 tcggggaggt acagggtgat ttgtccatgc tctttgacaa gcaatatatc ctgacagaaa
    11041 acgtcgtggt agaaacccgt taccgcaccg aaaccgatac atggacggac gcggagggca
    11101 acacccatac agacacctac caagtgccgt atgactatta catctgctac gtcacgctgg
    11161 aaaacttcga cctgtcccat gtgccagtct atatcatgga cgaggaaacg ctaagtttct
    11221 attccctgta tatgtccacg ctaggaaacc ggcctgatct gttttccggc agccagtacc
    11281 ccaacgcctc caccctgacg ccgccgacct actacgacgt acccccggag gctttggagg
    11341 atgccacctt tgccgccatg ctcaaagagg cggaaaaata tatcggttat ccctatgtgt
    11401 ggggcggcag ctctcccagc accagctttg attgcagcgg cttcatttcg tgggtaatca
    11461 atcatagtgg atggaacgtg ggacggctgg gggcgcaggg cctatataac atctgcaccc
    11521 ctgtttccag ttcgcaggcc aagcccggcg accttgtatt tttcaaggga acctacgaca
    11581 cccccggcgt gtcccacgtc ggcctgtatg tgggcaacaa tgtgatgctg cattgcggga
    11641 acccgatttc ctacacgaac cttacttcaa actactggca atcccatttt tacagttacg
    11701 ggcgtttacc ttaacgccag aaaggagctt tcaattatgg caaccaaact tgaccgcatc
    11761 gaaaaggaca tccagaaaac caagtctaag atcgcggagt ttcagaaaca gcttcgggag
    11821 ctggaaacgc agaaaaccga acaggagaat ttgcagatca tccagcttgt gcgcggcatg
    11881 aacatgaagc cggaggaatt tgccgcgttc ttacgcagcg gcgcaatgca ggccgcccct
    11941 gccgcaaccc cttaccatga acaggaggac aatgcccatg aagaataaga ccagacggat
    12001 ttcccgtatt ctccccgcac ttctggccgt gatgctctgc gtcacggcct ttcccgttac
    12061 cgcccttgca ggcggcaatg acccggcacc ggccccgctg ccggaggcta caaccgaacc
    12121 cgctacgggc ggtttggagc ctgaaacaga cgaaaccggc accgccacgg acgggacaga
    12181 aaccgaggcc gaaaaagata ccggccctct gaccggcaag gacatcaccg acctgttttc
    12241 cgcgctgttc ggttccaagg tgagcattgc cgccacggac gacggcatcc agatcaccac
    12301 cggcaccgac aaaaaagaac ctgaacagac cggcaccgtc accaccaacg gcggcaggct
    12361 gaatgtccgc accggcgcgg ggcttgacaa gaccgcgttc acccagcttc ccaatgggac
    12421 gacggttgag gtagtcggca cagacggcga ctggattaaa attctcttgc cggagcgcat
    12481 cggctatgtc cattccgact atatgaccgt cagcgaaaag gaagtagcgg ctaccggcga
    12541 gggcggtttt tccctctcca ttgacccgga ggaaattgct tctctgctgg aactgttcaa
    12601 tggcggcggg gccagcgccg ccctgtcccc ggacgggaac ctgtcactga ttgacgacat
    12661 cggcagcgcc gcgaaaaatg gcaagcagtt tatcaccgtg gaaaccaaaa acggcaatgt
    12721 gttttatctg attattgacc gggacgacga gggaaaagaa actgtccatt tcttaaatca
    12781 ggtggatgaa gccgatctgc tgacgctgat ggaggacggg gaaaccgtca cccccgctgc
    12841 tgtctgctcc tgtaccacga aatgcaaggc cggggctgtg aacacgaatt gcccggtatg
    12901 taaaaccaac ctgaccgagt gcagcggccc ggagccgcag gaaccccagc cggaggaacc
    12961 cgaagccccg gaggaagaac ccaagggcgg cgcgggcggc ctgatcgtgt tcctgctggt
    13021 ggcgctggcg ggcggcggtg cggcccttta ctatttcaag ttcaaaaagc ccaaggctga
    13081 aaccaccggc agcgacgatt tgggcgagta tgattttggg gaggacgagg atttggagga
    13141 cgaggaaccc gaaaccgagt ttgacccgga agctgatttt gtgagtgaac aggaggacga
    13201 ggaatgacat ataaacttgt aatcgcggaa aaacccagcg tggcccgcag cattgcgggc
    13261 gtgatcgggg ccacggagaa acacgacggc tatttggagg ggaacggcta tctggtgagc
    13321 tggtgcatcg gacatctggt gtcctttgcc gacgccggac gctatgacga gcgtttcaaa
    13381 aaatggcgct atgaggattt gcccattctc ccggaaacat ggcagtacat cgtccccgac
    13441 gacaagaaac agcagtttga cgttctctgt tcgctgatgc agcgccccga cgtgaccggc
    13501 cttgtgtgcg ccaccgacgc cgggcgcgag ggggaattga ttttccgttt cgtctaccag
    13561 atggcgggat gccaaaagcc ctttgagcgc ctttggattt cctctatgga ggacagtgct
    13621 attaaggacg gctttgccca cctgaaaccg ggcggcgact atgatccgct gtatcaatcg
    13681 gccctgtgcc gggccaaggc cgactggctg atcggcatta acgccacccg gcttttttcc
    13741 gtcctgtacc acaagaacct gactgtgggc cgcgtccaga cgccgacctt aaaaatgctg
    13801 gtggacaggg acgccaagat taccgatttc aagaaagaga aatatcacat cgtccatatc
    13861 acggcgggcg gcgcggacgc ggtaagcagc cgcttttccg acgctgccga ggcaaacacc
    13921 gtcaaggcgg cttgtgcaaa agcgcaggcc gtttgcgttt ccgtgacgcg ggagaaaaag
    13981 acggaacagc cgcccaagct ctatgacctt accaccttgc agcgggaggc taaccggctt
    14041 ttcggctata ccgccaagca gacccttgac tatgcccagg cactgtatct aaagaagctg
    14101 ctgacctatc cgagaacgga tagtcaatat cttaccgacg atatgctgcc cgccgtggaa
    14161 agccttgtgt ccggcctgtg ggagctggtg ccgtttgcca agggcctgaa cctttccccg
    14221 cagtttgacc gcatcctgaa cagcaaaaag gtgtccgacc accacgccat tatccccacc
    14281 gctgaatttg tgaagcaggg ctttggcact ctggccgaga gtgaaaaaaa gctgctgtcc
    14341 ctgatctgct gcaagctctt atgtgccgtg gccgggccgc atatctatga ggccgtcacc
    14401 gccacattta cctgtgccgg gcaggagttt accgccaagg gcaagaccgt cctttccccc
    14461 ggctggaaag agattgaacg ccggttccgt tcttccttaa agaccgacgc cgacgaggac
    14521 gccgaggcgg tgcgggagct gccggagctt tgcgagggcc agacctttgc agacgtggcc
    14581 gcctctgtca ccgagcattt caccacgccg cccaagccct acaccgaaga tacgcttttg
    14641 tccgctatgg aacgggccgg ggccgaggat atgccggagg atgccgagcg caagggatta
    14701 ggcacaccgg ccacccgcgc cgccattctg gaaaagctgg tgcagatggg ctttgtccag
    14761 agaaaaggca agcagctcat tcccaccaag gacggcatca atctggctgt ggtgctgccg
    14821 gaggcgctta cttctcccca gcttactgcc gaatgggaaa accggctgac cgagatcgcc
    14881 aaggggcagg ccgatcctga cgaatttatg gacggcatcg agacgcaggc gcgggagctg
    14941 gtgcagacct attcctgtat cagtgaggaa aagcagaaat tgtttcaggc cgagcgtgtt
    15001 cctatcggga cgtgtccccg ctgtggtgag gctgtctatg agggcaagaa aaactactac
    15061 tgcggaaacc gggcctgcca gttcgtcatg tggaagaatg accgattttt tgaggaacgc
    15121 aagaaagcgt tcaccccgaa gatcgccgct gcgctgctca aaaccggcaa agtcaaggta
    15181 aagggcctct attccgtaaa gaccggcaaa acctacgacg ggacggtgct tttggctgat
    15241 accgggggca agtacgtcaa ctaccgcatt gaacacagga gctaaaggac agtaacacag
    15301 caagcatagg gagtgtggct acactcccgg taaaatcccc cggccgcgtc cctgccgggc
    15361 cggggatttt tgcttgttgg ggaagtcccc aaccccctat tttttgaaac agaacagaga
    15421 aaggagtgac cgcgtgaacg aagtattttc tatccaagtc cataaccggc agtttttgga
    15481 acagggcaaa caaggcgtct ggctggattt gcccaccaca accgaaacgc tgcaagccgc
    15541 cctgcgggat gttggcatta ctgccgacaa tccgcaggat ttttttatca acgggtattc
    15601 ctgcccggag gacagacacc ttgccattcc ctatgacatg gtgctggccg ccgacgtgga
    15661 cgagctgaat ttccttgccg cccggctggg ccagcttgac gccgccgaga tcgcagagct
    15721 gaacgccgcc ctgcaaaatc cccgtggcgg ctttgagaat atcggccaga tcatcgacta
    15781 caccgaaaat gtggattact atgtgcatct tcccgacgtt aggggggatg gacagttggg
    15841 cgactactac ctgaaccgtt ccggcatggt ggatatgccg gaggaatgga aagccgggat
    15901 tgatacggcg cagtttggac gccacattgc ccagcaggaa caaggcgttt ttacccagta
    15961 cggctatctg gtgagaagcg gcgacgaatg gcagcgcgtc catgagggcc agccggtgcc
    16021 ggaggaatac cgggtgatgc actaccccca gccggaagct gaccgggagg aaccgcaggc
    16081 ccagcagccg gaggcatccg aaagccccga caaagcgccg cccccggtta tcccgatcat
    16141 gctggacggc atgaacagca aagaacgcat gaaagtgatt actgaccgtc tggaaaccgg
    16201 cgtacaggag ctttttgaaa gtagccgcta tatggcttac ttatccgcta tgtccaagat
    16261 gcacgattac agtctgaaca atacgctgct gatcgccatg cagggcggtt cgattgtggc
    16321 gggctataac aggtggcagg atgaatttga ccgccatgtg aagcggggcg agaaaggcat
    16381 taagatactg gccccggccc cgttcaaggt taagcagcag atggagaaaa ccgacccggc
    16441 cacccagcag cccgtgatcg gcgcggacgg caagcccgtg accgaagaac aggaagtgac
    16501 ggttccggcc tttaaggtgg tgacagtctt tgacatcagc cagaccgagg gcaaggattt
    16561 tcccatgccc tatatcagtg aacttaccgg cgacgtgcag cagtatcagg atttctttgc
    16621 cgcgctggaa cggacatcac cctatcccat aagcattgag ccgatttcag acggggccaa
    16681 gggccgctgc ttctacgcaa aacagcgcat tgccatcaac gagggcatga gcgaattgca
    16741 gaccctaaag accgccatcc atgagattgc ccatgccaag ctccacgcca ttgaccggga
    16801 ggcccccgcc gaggaacagg cggcccggcc agaccgcagc acccgcgagg tacaggccga
    16861 aagcgtggcc tataccgtct gccagtatta cgggctggac acgtcggact attcttttgg
    16921 ctatgtggcc gattggagca gcggcaggga gttatccgag ctgaaagcct cccttgaaac
    16981 catccgggcc acggccagcg aattgataac cgagatcgac ggccattttg cccagcttca
    17041 gcaggagcgc gaggcccggc aggaacagga ggccgtcacg gttgacgtta ccccggaggc
    17101 agcggccccg gcgcaggccg aggaagaaca ggccccggcc cttgatccgg cagccgagcc
    17161 gcttgtgact gtgctatgga gcgaaagccc ccagctaaag gacggccagc aaatgccgtt
    17221 gcatgaggcc gacgcgctgt ttgcccgtct ggatgcagca tatccggcag aggcaggcta
    17281 tgacaaaacc gcgttccgca ttgactgtat ctttcagggc gagccggaca gctacgaggg
    17341 gcggcaggat tttggagata gggacggttc cctgatcgaa catatccaga gctaccacca
    17401 attttacgcg caggacgaaa actggaaaaa gcatgtaatc gctacaaccg gcatagaggg
    17461 ctgggagcag gacaaggccc atcgggatat gctgttaggc gagtttgtcc cctatatgaa
    17521 gctgcattgc agccttgccc aaatggaaca gacggcgcag ggccgcttac agtccgggga
    17581 aaccctgacg ccggaggaaa cggcctattt gggggcgctt ctctcttatg tgcaggagtg
    17641 ccgccccatg ctcaatcagg gccagtatca gttgccggag ccgccgaaac tggctgactt
    17701 cgaccagagc ttgcaggact acaaagcaca ggtacaggcc gagatcgcgc aggaggccgc
    17761 cgcagccggt atgaccgtgg aggaatacgc ggcggctggc tatgaagcac cccaacagga
    17821 caccttttcc atctaccagc ttcgaggcgg ggaggaaacc cgcgactatc ggtttgagcc
    17881 atacgagcgt ttacaggccg cagggctggc cgtggacagt gccaactatg accttgttta
    17941 tacggcaccg ctggataaaa gcacgtcttt ggaggatatt taccgcacat tcaacatcga
    18001 ccacccggca gattttaggg gacacagcct ttccgtttcc gacgtggtgg tgctgcacca
    18061 gaatggacag gacaccgccc actatgtaga cagcttcggc tatatggaag tgccggagtt
    18121 tttgcgggaa aatcccctgc gtaccgccga actgtctacg gagcagaacg aaaacatgat
    18181 tgacggcatt atcaacaata cgccgcccga accgcagccg gaggaacccg aacagctatc
    18241 ttttgaggat gtggcagttg cttccggcca gacacaggag gaacccccgg aggctgcgcc
    18301 cccgcaaacg gctgtccgct attatcccat caatgaggga gccgcccgcc gcgccaagga
    18361 agccatgagc ttttctgact accggcccgg cagcgccacg gcggaatacc ggcactatgt
    18421 cgatcaggcc gtggaaattg cggagcgcca gaaaaagcgt gtggagccag agtatcacga
    18481 aaagatagac agcctccttg acacctacgc ccgcaagctg gccgagaaca tgaataaggg
    18541 ctatgagatt accgcccgcg tcccctctgt cctgattgcc gggccgtcca atttcccggt
    18601 acgggcgaaa gaaaagcaga acgccgccag tgaccgcaac atggaggaat ttcagtacat
    18661 tcagggcttg cttgacaaaa tccgcagcac cggcatgggt ggtatcagcg ccgacgatcc
    18721 gcaggccgtt tccaagctgg aaaagaaact ggaaaagctg gaagcgtcgc aggagttgat
    18781 gaaggccgtc aatgcctact accgcaagca tgggacgctg gacggatgcc cccatctgat
    18841 cgaaagaggc attgaaaacc tgaaagcgga tatggcgtcg ggctggcact atgagaaaaa
    18901 accgttccag tcctggcagc tctccaacaa taacgccgag atacgccggt taaagggccg
    18961 cattgaggaa ttgacccgcc aaaaggaggc ggcctatgtg ggatgggagt ttgacggcgg
    19021 cactgtggag atcaaccggg tggccaaccg cctgcaaatc ttctttgagg ggaagccgga
    19081 tgctgctgta cgcgacgagc tgaaaagcaa cggtttccgc tggtcgccca aggccggggc
    19141 atggcagcgg cagttaaacg acaccacaat ccgcgtcgct gaccgtatca agtgcatcca
    19201 gcccctttcc ggcgaaaagc ccagcgcctt acagcttgcg gcccgcaggg agaaagaaaa
    19261 gccctctatc cgggcgcagc tcaacgcggc caagaccgaa caggccaagc agccaccggc
    19321 gcgggaaaaa tcaaagggat tggaggtaga gtaaatgcag aagtttgaaa atgtggacgt
    19381 tctggccgcg ctggaacaga tcatgcggca gaacaccgcg ttttatcaaa gtgactttgg
    19441 cattgacaaa gacattatca ggcgggatgc cgccagcgat caggccgcag acaagaccct
    19501 gctctggatg tcccggccct ccgggacgta ctgtttccgg gaacgtgatg ttttcctgaa
    19561 agacacccga cagtacaaca catggaaatt ttacggggag cagacccgtg acaagatact
    19621 ggcctacgcg gtggagctga ccggcacaca gggcggcact atccggggca atctctatga
    19681 gctggactat ccgcagcatt tccggcacgt cattgaaacc gcccagccgg tgagtgtgaa
    19741 ccggctcttt tatgagcatg gcacccgtga catcccggag agccagcgtt ttgacggaag
    19801 ccctgaccgg gtattgggaa actttttgta ctatgaggcc cagccccatg acccggctgt
    19861 gctgcaggag gcgctacgga cggagcagca cagccggggg cgggccgtcc ccggcgattt
    19921 caaagtccac gtcaccgctt tgcatgacag ccggattgaa accgaggccc ggcgtattgt
    19981 ggacagcctg aaagatctgt ccgcgcctaa cagccccaat aaaacgcatt tcatggtgga
    20041 gatctccccg cactttgcgg cgattgcttc caccaaggat aatgaccggc tctttgccat
    20101 gctgccctat aaatccctgt gttttaccgg gatgaaaggc aggcatggcg tgtatgccgt
    20161 gattaaccgg gacgagcgcc gggatgtgag tattcgcagg ccccggccct ctatccgcaa
    20221 gcagctatcc gacagcaaac aggccgccag cccaaaaaag gcggcggcca gaaccaaaaa
    20281 gaatgaattg gaggtataag gaatgatgca ctttaccgtg gaggaagaaa acctgatctg
    20341 tatgtaccac aatgcagacc ggcgcagaac gataaacagg ctcaccgccg tcatgccggt
    20401 tatggatggg gatatgcggg cgcaggccca gcagaccctt tccaagctgg aacgcatgac
    20461 ggatgccgac tatgacggcc agaagttcca cttcacagac gaataacgaa cagcagacgc
    20521 cgggcgcggc ggccatgtgg tcgcttgcgt ccggcgtctt ttctttttgc ctattcctac
    20581 gcatttagaa cccggtattg tcggggatag gatgaagtat tcgcttgccc ctgcaaacat
    20641 gcgggagaag ccttgcagcg ggcggttttc aatcccttaa atgcgtaggt tgcagctata
    20701 attagacccc tgaaacagat atagccgcgc agcgggtgta tctgtttcag gggtgtgcag
    20761 gtgatagccg caaagcggcg taagagggtg cagccctctt gccgtctatc tctgttccag
    20821 cgggaagctc ggcaatgctt ccagcagttt cagggaaatg cgctgccgta tatcttcgtc
    20881 aatgactttc ttctttttgc cgtttgcctg tctgatctcc accgtggaaa gctcgttgat
    20941 gtagtcagca taatgggcca acactttttc cgtggcccac ctctcaccgg caacggcggc
    21001 tttgatcgtt tcatagtcca gcagcttttc ttgtccgtcc atcctgatac ccccgtcaga
    21061 ctgatttttt ctatgatacc atattcggaa gcatggaacc agagaaaacg gcgcttttct
    21121 gctggtaaat tctacgcatt tagaacagca cttcggcggg gataggataa aacacccaca
    21181 taccccgctg accatgccgg aaaagccttg aaatataagg gcttttacag ctctaagtgc
    21241 gtaggtttcc agtatctttt ccgctggcgc tcggcctctt tgcggcggcg catccgtttg
    21301 gagcaggccg cgcagtattt ggcccggtta gacttcggcg tgaacgtggc cccgcactca
    21361 cagcagcgtt tcacttcgtc ccggtctttg gtgatctcgg catacagggc cgcatccagc
    21421 gggaggacag cggccttaaa ccagcggcac agaagcgaat aggaaatgct ctgtacgcaa
    21481 atacattctt cgccgtcgtc cagcaacagg cagttgccgt cacaatagtt gcagcactca
    21541 tgcgtcagct tccgggcctt gcgatattga gggtaattca ttcgtggaat actcatagct
    21601 cttgcgtcca ttcctttccc ggcgtcatac gcaaaatgcc gtccacattc tgcttgatga
    21661 tgccgtattc ctgcatctgc tttttcgctt cgccgtactt ctcatacagc cgttcctttt
    21721 cctcggccaa tcgcttgtac tcggctttca gggcgtacag atcggggagc tttttcacac
    21781 cctgtccctt tagggccttt gccgcagctt catacaaaat caacgtgctt tcatgctcac
    21841 gccggaattt tgccttatcc gctgcattgc ggtattccat agccacgggc cagagctgct
    21901 tgtaggtaga aatattcttt atcagcaccg ccatatccgc aagccggtgt tccatagctt
    21961 tgagggtagc ggcggcctcg tcgctggcgg cggtgatctc ggccactttg ctttccaaat
    22021 ccgggtaggt gtctatccca tgttcagtca ggaaattcaa agtctttgcg gcctgcttca
    22081 aattgtggag cttcgcccac ctctcatagc ctgccgactg ctgggcctta atgttgtttt
    22141 ccagatcaat ccgcaaagaa atccccttgc gctctttggg cgctctggtg ataacgcggc
    22201 ctttgatacg ctctttgatg gcttcctcgg tgtaggcttc ccccaatgtc ttgcagcggg
    22261 taaagcgttc ctgtccggga gcgcggaaag aaataaactt tcccggcttg atctcatagc
    22321 cctgcgcctg catgagccgc aggaaatcat caaaatcttt tgcctgcggg atagcggcgt
    22381 caatggctgc tttgagcttc gctttccagc tcgtcccttt ccgctgggcg tcccactcgg
    22441 catagctctt gcccttactc tggccgggct tcaccacgga caatccgttt tccttgcaca
    22501 gcctgtcgct ggtgcgccgg atataggcat agctctgttt gttggaaatg tacttgcgct
    22561 gatttaccat atccacggca cagaaaataa tgtgattgtg gacgtggccc ttatcaatat
    22621 gggtagtcag cacataggga tatttccctt gcagcacttc ctcggcaagc tgcttcccga
    22681 tctcatgggc ctgttccggc gtggcttcgc cgggggcaaa ggattgtatc aggtggtggc
    22741 ccagattatt gcccttttgg tatgcctgcg ccagcagcat ttcaaattca atgtccgctg
    22801 tttcataaga gcaggcaaag gaggccacca gcagcttgcc gtcagttttg tccgggttct
    22861 cgatgtagtc aagggctttt ttcagcgttg acttgatagg atgtatcttt gtaactgcca
    22921 tatctgcgcc agcgccccct ttatatcctc gatgtcctgt gcgtacaccg gccccatacc
    22981 gttcagcctc tttgcaatct gattgacgtt gacgccgatc ttctgtatct cggcggtctg
    23041 cgcccggatg tcgctggtgt ccatgttgac gatatagccg tcaatgagca tcttccgggc
    23101 gtaggctgaa aagttcttcg tatgcagcag cgccattttc tgttcaatca tagcccgttc
    23161 ctccggcgtc acagggacgc gcagcacgat ttttcgcttt ctgtttgcca tgcttcaccg
    23221 tcctttccat tcaggggttt ggggatttcc ccaacaagca aaacggacgc ggcccggcag
    23281 ggacggggca ggccgttttt ccgggagtgg ctacactccc tatgcttgct acgttactgt
    23341 ttctccacta acaagacgtt tccaaagccc caaactaccc gcaaaatagc cctctggaaa
    23401 gaaaaaagtg caaaaaaata gaagctacgc tgtgcagctc ccgctaaaag taaaacagag
    23461 gatgccagtt acacatcctc cgtttcattc ttcttcggct tttcaattag tatggcgctg
    23521 tcaatcgcgc cctcgatgat cggcaggtat tcttcggggc agagtttcag cttgtggctg
    23581 accctttgcc gcagttcgct ttcctcccgc atgatctccg ggttaaagta gcgttccaca
    23641 ggaagcccgc aggctttaat aagctgtatg acaaccggca gactgggaat ggcccgtcgt
    23701 tttcgatgtt ggcaagatag cgccagtcaa tatttatttt ctctgccatt gctttgcgcg
    23761 aaacgcgctt tgcttgccgg gccgctttca catccgcgcc gaaagtttca aagccgggac
    23821 aatcttcaat attcgccata tcgcacctcc cacttacatt gtatatttca tactctgccc
    23881 gtggaatgtt ggtatatgca cctttcttaa agtttatatt tcacattttg tttcttttat
    23941 tcttcgagca aaagaattat aatgtaccct gtggaggtgt actatggact acatgacgtt
    24001 gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg ataaactact attgtgctgc
    24061 gggacgcatc cccggcgctg taaagatggc gacgatctgg ctcattccca aaactgcgga
    24121 aaagccgatt gacaggcgta caaagaaagg aaaggcggtt actgaatagt ataatgaaac
    24181 cggcgcattt tggatatggt tttgaagaga gagaaagcat ggtattttgt cacggaaaaa
    24241 ctataagatg atagatggca ggctgctcca gaccgataaa aagtattctc atctgaaaca
    24301 gaaagaaaag atttacacat ggatgtatga agaagcgaaa ctttatcatg acagaaatgg
    24361 aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc agccgtatat ggtagaattg
    24421 aaaaggccgg catctggatt ctttatggtg aagtctccag acattggata actgctggtc
    24481 taatgcgcct ttgagtatgg ggtgattgat tttaataccg gggaggtacg gctttttgag
    24541 gaaaatattc tgggatttac atttggcgat tattacattt cctgtaactg caaggacacg
    24601 gatgatgaag atatgaggaa tatagacttg ctgattttct actgtcttga acggtattag
    24661 gactgaatct gctgcgcgga gtgcggcaag agcgaccgga gggagtacac acaggccctg
    24721 cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa agaggccgcg cattttgctg
    24781 aggcttgggg tgtccccaac aagcaaaacg agaacgccgc aggcgggcaa gtggtggatc
    24841 agaatatccg taaaggatat tctgatccat tgcttgccag tattatcggg atgttttgac
    24901 aaccgctgta atcgaaacat cccaaacatt aagaaggtgt taattattat tatcttgatt
    24961 taattcgttt aaaccaataa tatagataca tacaccatga aaagaagcga gtatcccaat
    25021 gacggatgat taaaatccga aaagaaatcg ctttgaaact acagggaaac tacagactgt
    25081 tatgttatct tcttaaaatg gagggatttt tatgtcgata cgaattctac ttgtcgagga
    25141 tgatgatcat atctgtaata cagtaagggc gtttctggct gaggcaggat atcaggtgga
    25201 tgcctgcaca gatggaaatg aggcatacac caagttttac gaaaacactt atcaactggt
    25261 tattcttgat attatgctgc ccggtatgaa cgggcatgaa cttttgcgtg aatttcgtgc
    25321 gaaaaatgat actcccattc tgatgatgac agccctgtcg gatgacgaaa accaaatccg
    25381 ggcgtttgat gcagaggcag acgactatgt aacaaagcca ttcaagatgc agattttact
    25441 aaagcgggtg gaagccctgc tgcggcgcag cggtgcgctg gcaaaggaaa tccgtgtcgg
    25501 caggctgaca cttctgccgg aggatttcac ggtactttgt gacggtacag agctgcccct
    25561 gacacgaaaa gaatttgaaa tccttttgct gctggtgcag aacaaaggca gaaccttaac
    25621 gcatgaaatc attttgtcac gtatatgggg atatgacttt gagggtgatg gcagcacagt
    25681 ccacactcat atcaaaaatt tgcgggccaa gctgccggaa aatatcatca aaaccattcg
    25741 cggtgtagga taccgattgg aggaatcata ataatggaaa gaaaagggat tttcattaag
    25801 gttttttcct atacgatcat tgtcctgcta ctgcttgtcg gtgtaacagc aacactgttt
    25861 gcacagcaat ttgtgtctta tttcagagtg atggaattac agcaaacagt aaaatcctat
    25921 cagccattgg tggaactgat tcagaatagc gataggcttg atattcaaga ggtggcaggg
    25981 ctgtttcact acaataacca atcctttgag ttttatattg aagataaaga gggaagcgta
    26041 ctctatgcca caccaaatgc caatacatca aatagtttta gacccaactt tctttatgtg
    26101 gtacatagag atgataatat ttcgattgtt gctcaaagca aggcaggtgt gggattgctt
    26161 tatcaagggc tgacaattcg gggaattgtt atgattgcga taatggttgt attcagcctt
    26221 ttatgcgcgt atatctttgc gcggcaaatg acaacgccga tcaaagcctt agcggacagt
    26281 gcgaataaaa tggcaaacct gaaagatgta ccgccgccgc tggagcgaaa ggatgagctt
    26341 ggcgcactgg ctcatgatat gcattccatg tatgtcaggc tgaaagaaac catcgcaagg
    26401 ctggaggatg aaatcgcaag ggaacatgag ttggaggaaa cacagcgata tttctttgcg
    26461 gcagcttctc atgagctaaa aacgcccatc gcggctacaa gcgttctgtt ggagggaatg
    26521 cttgaaaata tcggtgacta caaagatcat tctaagtatc tgcgcgaatg catcaaaatg
    26581 atggataggc agggcaaaat catttccgaa atactggagc ttgtcagcct gaatgatggg
    26641 agaatcgtac ccatagctga accgttggac atagggcgca cggttgccga gttgctgccc
    26701 gattttcaaa ccttggcaga ggcaaacaac cagcggttcg tcacagatat tccagccggg
    26761 caaattgtcc tgtccgatcc gaggctgctc caaaaggcac tatccaatgt catattgaat
    26821 gcagttcaga acacgccgca gggaggcgag gtacggatat ggagtgagcc tggtgctgaa
    26881 aaatgccgcc tttttgtttt gaacatgggc gttcacattg atgatactgc gcttccaagg
    26941 ctgttcaccc cattctatcg cattgatcag gcgcgaagca gaaaaagtgg gcgaagcggt
    27001 ttaggacttg ccatcgtaca aaaaacgctg gatgccatga gcctccagta tgcgctggaa
    27061 aacacctcgg atggcgtttt gttctggctg gatttaccgc tcacatcaac attgtaaata
    27121 tttaaaactt aaatgatttt gggctctttg tcaaatagga ctgatagagt gagataagaa
//
LOCUS       DACCXM010000323        10087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.guided.098, whole genome shotgun sequence.
ACCESSION   DACCXM010000323 DACCXM010000000
VERSION     DACCXM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.guided.098"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            537..1139
                     /locus_tag="IVY61_003034"
     CDS             537..1139
                     /locus_tag="IVY61_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7972055.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1155..1832
                     /locus_tag="IVY61_003035"
                     /pseudo
     CDS             1155..1832
                     /locus_tag="IVY61_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2344..2640
                     /locus_tag="IVY61_003036"
     CDS             2344..2640
                     /locus_tag="IVY61_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP7972056.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            2641..3057
                     /locus_tag="IVY61_003037"
     CDS             2641..3057
                     /locus_tag="IVY61_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP7972057.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3077..4621
                     /locus_tag="IVY61_003038"
     CDS             3077..4621
                     /locus_tag="IVY61_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7972058.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4764..4988
                     /locus_tag="IVY61_003039"
     CDS             4764..4988
                     /locus_tag="IVY61_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7972059.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5003..5872
                     /locus_tag="IVY61_003040"
     CDS             5003..5872
                     /locus_tag="IVY61_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7972060.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5853..6587
                     /locus_tag="IVY61_003041"
     CDS             5853..6587
                     /locus_tag="IVY61_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7972061.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6620..7528
                     /locus_tag="IVY61_003042"
     CDS             6620..7528
                     /locus_tag="IVY61_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7972062.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7525..8067
                     /gene="sat4"
                     /locus_tag="IVY61_003043"
     CDS             7525..8067
                     /gene="sat4"
                     /locus_tag="IVY61_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP7972063.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8160..8954
                     /locus_tag="IVY61_003044"
                     /note="aph(3')-IIIa"
     CDS             8160..8954
                     /locus_tag="IVY61_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7972064.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(9062..9250)
                     /locus_tag="IVY61_003045"
                     /pseudo
     CDS             complement(9062..9250)
                     /locus_tag="IVY61_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9230..9802
                     /locus_tag="IVY61_003046"
     CDS             9230..9802
                     /locus_tag="IVY61_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7972065.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         3548 a         1572 c         2207 g         2760 t
ORIGIN      
        1 acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt
       61 tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta attaattatt
      121 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
      181 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag
      241 ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta
      301 taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa
      361 atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata
      421 tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa
      481 agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg
      541 ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag
      601 cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc
      661 cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat
      721 tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta
      781 agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt
      841 taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac
      901 gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat
      961 tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt
     1021 tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa
     1081 cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa
     1141 aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct
     1201 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     1261 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac
     1321 caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg
     1381 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac
     1441 ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata
     1501 tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat
     1561 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     1621 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1681 tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta
     1741 cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag
     1801 caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt
     1861 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
     1921 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
     1981 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
     2041 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
     2101 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
     2161 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
     2221 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga
     2281 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
     2341 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
     2401 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
     2461 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
     2521 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
     2581 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
     2641 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
     2701 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
     2761 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
     2821 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     2881 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     2941 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     3001 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     3061 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     3121 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     3181 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     3241 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     3301 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     3361 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     3421 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     3481 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     3541 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     3601 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     3661 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     3721 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     3781 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     3841 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     3901 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     3961 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     4021 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     4081 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     4141 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     4201 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     4261 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     4321 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     4381 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     4441 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     4501 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     4561 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     4621 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     4681 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     4741 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     4801 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     4861 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     4921 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     4981 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     5041 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     5101 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     5161 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
     5221 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
     5281 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
     5341 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
     5401 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     5461 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     5521 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
     5581 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
     5641 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
     5701 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
     5761 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     5821 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     5881 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     5941 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     6001 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     6061 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     6121 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     6181 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     6241 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     6301 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     6361 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     6421 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     6481 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     6541 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     6601 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     6661 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     6721 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     6781 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     6841 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     6901 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     6961 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     7021 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     7081 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     7141 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     7201 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     7261 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     7321 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     7381 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     7441 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     7501 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     7561 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     7621 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     7681 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     7741 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     7801 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     7861 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     7921 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     7981 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     8041 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     8101 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     8161 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     8221 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     8281 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     8341 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     8401 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     8461 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     8521 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     8581 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     8641 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     8701 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     8761 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     8821 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     8881 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     8941 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     9001 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     9061 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     9121 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     9181 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     9241 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     9301 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     9361 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     9421 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     9481 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     9541 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     9601 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     9661 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     9721 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     9781 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     9841 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     9901 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     9961 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
    10021 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
    10081 ttgtgat
//
LOCUS       DACCXM010000324         7943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.guided.121, whole genome shotgun sequence.
ACCESSION   DACCXM010000324 DACCXM010000000
VERSION     DACCXM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.guided.121"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            128..1564
                     /locus_tag="IVY61_003047"
     CDS             128..1564
                     /locus_tag="IVY61_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7972066.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4185
                     /locus_tag="IVY61_003048"
     CDS             1753..4185
                     /locus_tag="IVY61_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7972067.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            4415..5482
                     /gene="rlmN"
                     /locus_tag="IVY61_003049"
     CDS             4415..5482
                     /gene="rlmN"
                     /locus_tag="IVY61_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7972068.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5675..6778)
                     /locus_tag="IVY61_003050"
     CDS             complement(5675..6778)
                     /locus_tag="IVY61_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7972069.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7330..7878
                     /gene="aac(6')"
                     /locus_tag="IVY61_003051"
     CDS             7330..7878
                     /gene="aac(6')"
                     /locus_tag="IVY61_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7972070.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2658 a         1357 c         1724 g         2204 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaagttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     2521 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     2581 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     2641 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     2701 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     2761 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     2821 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     2881 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     2941 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     3001 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     3061 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     3121 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     3181 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     3241 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     3301 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     3361 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     3421 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     3481 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     3541 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     3601 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     3661 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     3721 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     3781 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     3841 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     3901 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     3961 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     4021 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     4081 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
     4141 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
     4201 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
     4261 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
     4321 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
     4381 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
     4441 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
     4501 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
     4561 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
     4621 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
     4681 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
     4741 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
     4801 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
     4861 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
     4921 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
     4981 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
     5041 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
     5101 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
     5161 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
     5221 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
     5281 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
     5341 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
     5401 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
     5461 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
     5521 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
     5581 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
     5641 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
     5701 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
     5761 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
     5821 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
     5881 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
     5941 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
     6001 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
     6061 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
     6121 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
     6181 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
     6241 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
     6301 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
     6361 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
     6421 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
     6481 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
     6541 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
     6601 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
     6661 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
     6721 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
     6781 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
     6841 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
     6901 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
     6961 gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa
     7021 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
     7081 ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt
     7141 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
     7201 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
     7261 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
     7321 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
     7381 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
     7441 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
     7501 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
     7561 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
     7621 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
     7681 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
     7741 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc
     7801 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
     7861 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
     7921 gaaggaattt acgaaaatta ctt
//
LOCUS       DACCXM010000325         5892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.guided.142, whole genome shotgun sequence.
ACCESSION   DACCXM010000325 DACCXM010000000
VERSION     DACCXM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.guided.142"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            332..898
                     /gene="amaP"
                     /locus_tag="IVY61_003052"
     CDS             332..898
                     /gene="amaP"
                     /locus_tag="IVY61_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7972071.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            911..1099
                     /locus_tag="IVY61_003053"
     CDS             911..1099
                     /locus_tag="IVY61_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7972072.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1112..1669
                     /locus_tag="IVY61_003054"
     CDS             1112..1669
                     /locus_tag="IVY61_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7972073.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1701..1943
                     /locus_tag="IVY61_003055"
     CDS             1701..1943
                     /locus_tag="IVY61_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7972074.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2284..3675
                     /gene="amt"
                     /locus_tag="IVY61_003056"
     CDS             2284..3675
                     /gene="amt"
                     /locus_tag="IVY61_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP7972075.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            4070..4543
                     /gene="dfrF"
                     /locus_tag="IVY61_003057"
     CDS             4070..4543
                     /gene="dfrF"
                     /locus_tag="IVY61_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP7972076.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            4670..5326
                     /locus_tag="IVY61_003058"
     CDS             4670..5326
                     /locus_tag="IVY61_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP7972077.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            5508..>5892
                     /locus_tag="IVY61_003059"
     CDS             5508..>5892
                     /locus_tag="IVY61_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7972078.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNM"
BASE COUNT         2012 a          864 c         1239 g         1777 t
ORIGIN      
        1 taagaaccgt caggtttagc taagtggtga gtagttcact tctccgaaaa aatttaataa
       61 ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa tcttgaataa
      121 tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc aattgttatt
      181 tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt agcgctttat
      241 ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag agttatttac
      301 agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa taatcggtgt
      361 tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca atgcgccgta
      421 tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt actacatgca
      481 acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca tcttgctagt
      541 cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag atggcaaact
      601 aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg aagaagagtt
      661 cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata tccatgtcaa
      721 cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga tgcaagatat
      781 cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg cagtcacata
      841 cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag tggaatagga
      901 gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct ttgggggtct
      961 tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat tgttgatcat
     1021 aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag gaatagttga
     1081 tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga atacaacaaa
     1141 tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg aattgactta
     1201 tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag atggtctatt
     1261 gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca cagataacgt
     1321 aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc tagatgtgat
     1381 cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat tgatcagtac
     1441 tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg ttgcagacat
     1501 caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag taacagaagc
     1561 agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat ctgctgtagg
     1621 caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag aagtaagaag
     1681 tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc ggtatcattg
     1741 gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc gcaaacatca
     1801 ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg ggaccaacaa
     1861 ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta ttagtcgttt
     1921 cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa atgaagaaca
     1981 aatttgagaa ccgtagcgac actcgctgcg gttctttttt atatatttta tatgaagtat
     2041 tctttattcc ttatgaaaaa atatctttaa aaataaaaaa agacgaaaat aaacggtttt
     2101 ctataaaaag aagaccgttt attttttgtc tttatttgtg tgaaaaacga aaaagagcac
     2161 aacacatcaa gcttttatta attgtctgtc ttttataaat gcaagtatta attcaaaaaa
     2221 acagaagaaa aaaacaacta caccttcttt tatactcgta atcgtagaaa ggaggcgaaa
     2281 aaaatgaata cattacctat caatacagga gatacagcat tcatgatttt atgtacagca
     2341 atggtttgcc tgatgactcc tggtctagct tttttttacg gcggattagc acggaagaat
     2401 aatatactaa cgatcatgtc tcaatctttt ctctccgttg ggataacaac gattatgtgg
     2461 gtatttggtg gattcggatt ggcctttgga cgggatatcg gcggtgttat tggaaacccg
     2521 gcagatttct ttttgatgag acatgttaca agcttcccca acatgtttca tggtgcaacg
     2581 attccatttt taatgttctt tctttaccaa ctgatgttta tcgtgattac cgttcctttg
     2641 atgacaggag cttttgcagg gagattgaat ttaaaaggct atattatact tgttattttt
     2701 tggaatctgc tgatttattt ccctgtttgc cactggatat ggggtggcgg atttttggat
     2761 cagatgggat ttagagattt cgctggtgga gcagtcatcc acacgacagc tggattcggc
     2821 tctttagcat gtatcctcac acttggtcaa agaaagattc ctttaggaga aagaagtcag
     2881 catagcagtc ttatggcagc tgctatcggt acaggactgc tttggtttgg ctggttcggt
     2941 tttaattccg gtggtgcact tcgagcagat gaacaagcag tcaatgcatt tggttcaaca
     3001 ttcatcgctc ttgcatttgc gatgattact tggctgatca ttgcaaaagt aaaaggaaat
     3061 ggctttgatt ttgtcgacgt actaacagga tccgttgctg gattggcagc cattacgcct
     3121 tgtgccggtt atgtagaagc aaaaagtgcc atgctgattg ggatcattgc gggtatcgtc
     3181 tgtcatgctg ctgttgattt tcgtaaaaag aaacaatggg atgatgcact tgatgtgtgg
     3241 ggtgttcatg gcatgggcgg attcacaggt acgattctga tagggatctt tgcatctaac
     3301 agccatttac taacgaatcc aagcagctgg tacttcttag ggattcaagt gattggtgtc
     3361 atcattactg ccgtgtatgc ctatgtgctg acaacagtga ttttaaaatc ggcgatgcac
     3421 tttacaacga tcactactac aaaagaagaa caagaagaag gattggactt tttcttcttt
     3481 ggctttttgg ctatgaccat ctcaatggtc gtttcattat atgaatatcc cacattcgcg
     3541 acctcaggct tcagcttagt atttttcttg ttattaggcg gtttgctatg gtttatccct
     3601 gtggcattgt gcgcagcaga gatggcaacg gtgaaaggat gggaaaaagg tgtttcccaa
     3661 acaaaaagat attaagaaac agcccatgtt tacctgcttg caaacaaaag aatattgcta
     3721 ctagactaag agatactaca taagtgtgtt ggtatctctt ttttgttgcc gtgaaacagc
     3781 agactatttc atttctgctt gattacctta aaacttatta gtctatggtt tgtcaagggc
     3841 ttgttgcgtg agaaatgctg aatatcaccc tttacaaaac ataggctgat aagtaacttt
     3901 tcaaaagcag aaaagaaatg gcaatgctta taacaatatg atataatgca gatgaagaca
     3961 aaaaaggaat ttgtcgagct ggttagttcg agataatttt gagtttgtta agatgtggtg
     4021 cacttgatgg aatcacaggt acgacatcat attatggagg atgaaataga tgataggttt
     4081 gattgttgcg aggtcaaaga ataatgttat aggcaagaat ggtaatatac catggaaaat
     4141 aaagggagaa caaaagcaat ttagagagtt aacaacgggt aatgtggtta ttatggggcg
     4201 aaagtcttat gaagaaatcg gtcatccgtt gcctaataga atgaatattg ttgtttccac
     4261 cacaacagag tatcaaggag ataatttagt ttcagttaaa tcattagaag atgcattatt
     4321 attggctaaa ggacgagatg tatacatatc tggtggatat ggactattta aggaagcttt
     4381 gcaaatagta gataaaatgt atatcacaga agtagattta aatattgaag atggagatac
     4441 attctttcca gaatttgata tcaatgattt tgaagttttg ataggggaaa cacttggtga
     4501 ggaagtgaaa tatacgagaa cattttatgt aaggaaaaaa tgaattgagt aaattttgga
     4561 tttagggtgt tttcataaat atattgcttt ttatgcatat ataaattgtt gtaagacaaa
     4621 ttccagtttg tatggggcga taaaatcaga atttgagaga ggattgaata tgaataagga
     4681 atggtctgaa cttaataaaa caatgcaggc acaaataaaa aagaaagata cttataagag
     4741 gggtattgat actttactta ctttacgaag tcagttaata caaaccttag tatctttcaa
     4801 agaagaatta tgcagagaag attttaactc aatccccttc ataaacgctg atggttatca
     4861 tagtaagacg attgcttatt ctatttggca tattttccgc attgaagata tagttgtgca
     4921 tacagtgata aatgaagatg aacaagtatt ttttgcaggc aattatcaag agcgtatcaa
     4981 ttcacctatc attacaacag gaaatgaact catgaaacag cagattgcag acttttcaaa
     5041 acagcttaat ctggaagaat tatatttata tattttcgaa gtatgggaaa gcactgagaa
     5101 aatgcttgaa cggttatctt atgatgaatt gaaaagaaaa ataccgaaag aaagaaaagg
     5161 atacttagaa tcgttgaatg tagtaaacga caatgaaaaa gcaatttggc tgattgatta
     5221 ttggtgtaat aaagatattt gtggactaat tcaaatgccg ttttcaagac attggattat
     5281 gcacacagaa gcctgtttgc gtataaagaa caagatacat tcataggcaa aaatttcagt
     5341 ttgtagaact aaggaaattg gaatttaagg aggaataaag tatggttagt gattttataa
     5401 gagcagcatt tccatggatt ttaatgggtt tatttgtagc aatcagttgt tcccttatga
     5461 gtaaaaaaga gaaataaatt cctgtttgta tttataaaag agaaaaaatg agaaaagtag
     5521 ttttatttat tgccatgagc cttgacggat atattgcgga cggtaatggt ggagtagctt
     5581 ggctaaatgg tcatggaaat gacaatgaaa acatcgacac ttatacagaa tttaccaaag
     5641 atatagatac tgtcttaatg gggtggaata cttctcatca ggttgttact gaactttctc
     5701 cgcaagaatg ggtatataat aaatttacaa cttatgtatt aacacataaa gagtgcaatt
     5761 cttcccaaca aattcatttt acaagtgaaa atcctgtttt attattggaa cggctaaaac
     5821 aagaagcagg aaaggatatt tggatttgtg gcggagcaaa tcttgttcag cagttggtaa
     5881 gcaaaaatat ga
//
LOCUS       DACCXM010000326         5740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.guided.147, whole genome shotgun sequence.
ACCESSION   DACCXM010000326 DACCXM010000000
VERSION     DACCXM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.guided.147"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            211..903
                     /locus_tag="IVY61_003060"
     CDS             211..903
                     /locus_tag="IVY61_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7972079.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1230..1727
                     /gene="dfrG"
                     /locus_tag="IVY61_003061"
     CDS             1230..1727
                     /gene="dfrG"
                     /locus_tag="IVY61_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7972080.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1799..3751)
                     /locus_tag="IVY61_003062"
     CDS             complement(1799..3751)
                     /locus_tag="IVY61_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7972081.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3758..4054)
                     /locus_tag="IVY61_003063"
     CDS             complement(3758..4054)
                     /locus_tag="IVY61_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7972082.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4396..4686
                     /locus_tag="IVY61_003064"
     CDS             4396..4686
                     /locus_tag="IVY61_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7972083.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            5400..5702
                     /locus_tag="IVY61_003065"
                     /pseudo
     CDS             5400..5702
                     /locus_tag="IVY61_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1956 a          913 c          992 g         1879 t
ORIGIN      
        1 ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc tctataaaaa
       61 agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta tttatattaa
      121 aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta cttttagcat
      181 gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac tggtgggatt
      241 gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa tgaaattatt
      301 ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca gcttgatcat
      361 acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc acaaaaagaa
      421 cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt aaatgaatta
      481 gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca attagtcagc
      541 aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga tgagatcaac
      601 caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact acaagaagca
      661 caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca aatcgcaaca
      721 ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa gaaattagac
      781 gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga aaaagcaaaa
      841 gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca tttgcgtaaa
      901 taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc tatactaagt
      961 agttcagttt tggagtacaa aatgacaaaa tgtacttcaa aatggactac ttttttcatt
     1021 ttaacttaca taccaaatta catacaaaat gatttataaa atgcaattga aaatgataat
     1081 tgaatgaaaa tttgtgattt tgtggtatta tgataatata acttatagaa aaaattgctt
     1141 attcaactaa cgggcaggat aatgagaaaa ggttttttga tagaaataag aaattagatg
     1201 gtaaattagt taaaaaggct gtgttataaa tgaaagtttc tttgattgct gcgatggata
     1261 agaatagagt gattggcaaa gagaatgaca ttccttggag gattcccaag gactgggaat
     1321 atgttaaaaa tactacaaag ggacatccga taatattagg taggaagaac cttgaatcaa
     1381 tcggaagagc cttacctgac agaagaaata ttattctgac gagagataag gggtttacct
     1441 ttaatggttg tgaaattgtt cattcaatag aagatgtttt tgagttatgt aaaaacgaag
     1501 aagaaatttt tattttcgga ggagaacaga tttataattt gtttttccct tatgttgaga
     1561 aaatgtacat cacaaaaata catcatgaat tcgaaggaga tacttttttt ccagaagtga
     1621 attatgagga atggaatgag gtatttgccc aaaaagggat aaagaatgat aaaaatccgt
     1681 ataactacta ttttcatgta tatgaaagaa aaaacttatt gagttaacgg gtgcgttaat
     1741 tgaagaagaa cagtaaagat aaagcacaag agcttgggat tactccaagc tctttttgtc
     1801 atttgaaata aattatatct ttccaatcaa attgtttatc tgtaatccct aatctttgag
     1861 caggtgtcaa aaccttttta tccgctgatt taaaagggcg acaaaagttg tagtaagttc
     1921 ttagaattgt aatcgcaaat tgagcatact tcggattgaa gttagcgtaa atatagctct
     1981 tcttatcacc acgagctgtt gttaatggtc tttcaagaga tgatatataa cgtcttatct
     2041 gttgaataaa gctattggtc gaatggtcat taacatttaa tagcatcttt gcgatttcgt
     2101 ttggttccag agcagataaa tccgttctac aatcaactga ataaaatcct ttatccttag
     2161 tggctaatgg atgtttgata gggttatcgg catatttgcg atatctttct ccatctttac
     2221 taatagcctc ttcgtggaat tgatgacttg ttagtaattc agttagataa tgagatgcca
     2281 aagtccgtag aaactttgtt ttgaatcctc tgatatctcc ccaatcgagc agttctgctt
     2341 ttgcttgtcc gaattctttt aagcattgtt ttctgctttt cgtcttgtta acttggcaaa
     2401 taaaatgatg tgcatcgctc aaccttaatt ctttcgtgaa tactctataa aaggcgtttc
     2461 taatagagaa gtcatcatca ctaatcattc gccattcgtc ggcattaacc atttgtttaa
     2521 ttaaccaata gtgagcagtt gtagtataag gagcatcaac atttaaacca tcgacaaatc
     2581 tactcctatt agtaattttc cctaattcgt gaagataagc attcctattt tcgctatcgt
     2641 tagcggatgg ttcttgtgga taataagacc aatctaacct atcattttta cgactaaaag
     2701 tattgagatg gtcttctttg aattttcttg tatcttcatt taattcatcc attgaaatat
     2761 tccaatcata ggcaacatca gaacggaaca catatcttga taagacctca gcagatacaa
     2821 ctatatacgt ttgcatatta aggtcttcaa accccgtata tttctttgaa ccttgtcctt
     2881 tcttacggac gttatttagg tagtagtgca tcttgtccgt attaagccac atctcgttaa
     2941 atttctttgt ttgaaggggt tgtgtctcat atcgttctaa aaactccaaa caacgcctat
     3001 acagccactc cagcttatga tagtaggttc caactccaat tcctaaaata tcacaagtac
     3061 gactgactga aacccttcca acaaccatct tagcgaacat tggaagaatg gtgttctttt
     3121 gttggtgata agtagtggtt tcttctcttt ttggtagaac attagtgaac ttcttacacg
     3181 ccttacattg atagcgttga gatttgcccc tacttttccc ccttttgtaa aattgcttcg
     3241 gctcattgaa tggggttgat tcttcttccg agcagccttc tttatgaaat tgataatctg
     3301 gctcaacatc ttggatgcta ttgatttcta taaggcgttt aatttcttca acaacagacc
     3361 aattagataa aggggttact gtacagtttt gcgatacacc tctgccaata ggattgtcat
     3421 tgcagtacat acctttatga cctttatctt tggaactacc agtcatttta tatcgacttg
     3481 gtttcccttt tacatctttg aatttgtgct gttctttccc aaaattacaa caaaacggat
     3541 tgatgcagta attatattga atgggatatt cagaaccgtt ccatttaaaa gacgttggca
     3601 agaacaacat tttgttatac cttgttgcaa atcgttttgg agttaataaa tcatattgtt
     3661 tagccctatc ttttaattct tcgtttgaaa tcggaatttt aacatctaca atttcatctt
     3721 ccttcgtggc taatctttta agcttggtca ttgtctatca acccactcta tctttgatac
     3781 ccattttttc aacaatccgc ttattacttc gcttgttggc aatccattca ataaacttct
     3841 tatcgtctaa aatgttcaat aagaattcat ccactgcttc gctttcagta cgctctgcat
     3901 attcagcgta ttgcttaact atcaccctta cttgctctga tatttcccaa tccacagact
     3961 tagcgttctt attttttggc tcaagaaatt gcataggaaa atgcacccct ttttgcttag
     4021 taaattgtaa ccattttaac acaccaattt gcattcctac aataattatt tcacgttcgc
     4081 atttttatat acattttaac ttacattttg tggtgtaaat taacaacaaa cgttgattta
     4141 acaataaaaa acgagtatca aattcagttg caatttgata ctcatttagg tttacatttt
     4201 cactttttgt actccaaaac tgaactactt aaatctatac tgctgcctct tttttttagt
     4261 acatgtcctt catcttcgca ggaagtgcca atatgatttt aagaaaggta atagacaaaa
     4321 agtcaaaaag ataaaacaga gtttcttctt tacaaaggga atgtatgttc gtataatggt
     4381 attgaggtga aggttatgga gaaaaaatat ggaacagtag ataagatccg tactttgcaa
     4441 gttagtgcca gaccgttgat ccgtttcagt ttaaatgggg tcaattgttt gattgcttcc
     4501 catagtttga acttcctagc tgaagtagac gaagggatga agttagtcgt tacaggttat
     4561 tataataata gaaagcaatt tgttgtgaga tcctatcatg tgttagggaa acccaaaatt
     4621 gtggtagaat atgagaaaag tctataccca agaaaaaagg tccgacaaga tgaaagaata
     4681 aactagaaaa caagcggctg agacaaaaat gtcctagccg cttgttttgg aaagatggat
     4741 gcagacgatg ggatttgaac ccacacgagc atacgctcac aagaacctca atctatatat
     4801 aactacatgt atttaagtat ttgtgatgaa tcactaaact ttccaaactc atttgaggga
     4861 tattgcttaa agattgaaca gatcaattcg aagccctatt agtattcgct caatgggggg
     4921 atcagaggcc ataaaaagag actagtcatt actagtctca ggtagtggaa ttgtaggatt
     4981 ttaaacgttc ctggagagta ccggtatgta actcgaagag gtgattatca tagtcgtaaa
     5041 agtaaattga agaagcctcg cctttcacac gagaacgtcc tggttcgaca gttaaaccta
     5101 acatttggat ttttgagaca aacgaatcta tatcttgttc gtcaatttta aaagcaatat
     5161 gattgtatgt ctttggcagt ttattcgacg aattctgcat tactgcaatc cataggtcct
     5221 taactaagaa aaatttttct ggatataatg aaaatttttt tgttgactat cgtagatttc
     5281 ttttgcatca aataaggtat ttaacaatta agtatttaaa agataacttt taagttatct
     5341 ttaagtaatg cttaaagcta tgttatactc tattaggaaa tataagaagg agtataaaaa
     5401 tgaacaaaca taatagaaaa ataacgaata acaaagaact gatcttgtta ctaataggga
     5461 tttggggtat ctttttactc tttaatatgt atatgccgac tatgagagcg gatgacgtag
     5521 tttatgcaag tagattagat gaacttggct atttgggtgc ttcgatagaa cattataaga
     5581 catggagttc aagaattatt atagaactat tcttaatgtt cttttctaaa cactttatgc
     5641 tatggaaatt attaaattct acgattatgt taggaattgt agtattactt tgcaagtatg
     5701 ttgagagtat aaaatatttt gtgtaaatga aaaaatccat
//
LOCUS       DACCXM010000327         5732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.guided.148, whole genome shotgun sequence.
ACCESSION   DACCXM010000327 DACCXM010000000
VERSION     DACCXM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.guided.148"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            74..970
                     /locus_tag="IVY61_003066"
     CDS             74..970
                     /locus_tag="IVY61_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7972084.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1120..2061)
                     /locus_tag="IVY61_003067"
     CDS             complement(1120..2061)
                     /locus_tag="IVY61_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7972085.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2187..3308
                     /locus_tag="IVY61_003068"
     CDS             2187..3308
                     /locus_tag="IVY61_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7972086.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3566..5068
                     /locus_tag="IVY61_003069"
     CDS             3566..5068
                     /locus_tag="IVY61_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7972087.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5135..5662
                     /locus_tag="IVY61_003070"
     CDS             5135..5662
                     /locus_tag="IVY61_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7972088.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1869 a         1019 c         1215 g         1629 t
ORIGIN      
        1 acgttttact ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag
       61 tgaggcggaa aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta
      121 ttttttgcct tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga
      181 attcattaaa tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta
      241 tggatttgat atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt
      301 atttttttgg aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga
      361 accggcaatc gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa
      421 acataatagt tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga
      481 tctatttttt ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga
      541 atttataaaa agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca
      601 acctggatat cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct
      661 ggaactgttg cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt
      721 tcaaaagaaa ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga
      781 aaatattttt ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc
      841 atttaagcca atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg
      901 atttatcact aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga
      961 aaacaaataa aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc
     1021 ttatggcatc atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga
     1081 accagctgct gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc
     1141 ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt
     1201 ttgtcctttt cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt
     1261 gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga
     1321 agcaatatgt tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga
     1381 agcaaattca gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc
     1441 agcaatatta caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt
     1501 tcgccaaatg gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa
     1561 gacttctaca actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat
     1621 ttctccatca cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt
     1681 gatcccaaat aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa
     1741 tccatttagt aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa
     1801 catatcatct aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct
     1861 ttcgatgatt tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa
     1921 gtcagcaatc aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa
     1981 gaggacctca tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc
     2041 cccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta
     2101 tatagaaaaa tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt
     2161 acttaagaag aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag
     2221 gaagtgcttc ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag
     2281 gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt
     2341 acgcgctcag gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa
     2401 aaatcagcta tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac
     2461 aagcattggg gttagatcta cttgataaac caacggatca aactatccga cttgcttcca
     2521 gttatgcctt aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg
     2581 tcactttagg aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg
     2641 cttctttaga agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg
     2701 atcgaagaaa cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc
     2761 catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata
     2821 cgacgctagc tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct
     2881 gggaggtgga tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc
     2941 tttttttgtt gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat
     3001 tggaaaaaaa gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc
     3061 aaacgatcca cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac
     3121 caataatcgc tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc
     3181 caattgtctt aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc
     3241 aaacattaga aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc
     3301 cgaagtagtt gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt
     3361 gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct
     3421 tgtggagaca aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg
     3481 ggtctttttg tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg
     3541 gtcttttttt acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg
     3601 ctacgacagc caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg
     3661 gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca
     3721 aaacaaacta ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca
     3781 acagacaaaa gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga
     3841 gatatgggaa atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag
     3901 agaattcagc acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt
     3961 ggatgacact catttcccgt taatcgatga accaacgaat catttggata tctctggtag
     4021 aaaacaagta gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga
     4081 ccggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga
     4141 actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga
     4201 aatggctcaa aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga
     4261 aaaagccgaa tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat
     4321 cgatactgaa tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat
     4381 gaaacgatcc aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact
     4441 attaaaagat atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa
     4501 gcgactttta agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat
     4561 tcattttaca atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa
     4621 gtcatccatt atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata
     4681 ccagccaaaa catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac
     4741 gttggcggaa tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg
     4801 aaagcttggg atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca
     4861 acggaaaaaa gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga
     4921 tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa
     4981 cgtgaaacct gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac
     5041 tgagattatt tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt
     5101 taaggggaac tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga
     5161 aacaacttgg aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa
     5221 aggacagatg caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac
     5281 gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg
     5341 tctcagcttg ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga
     5401 tggactgcca agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt
     5461 cccaaatggg gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg
     5521 catcgaagac gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat
     5581 caatgcttat tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac
     5641 agaattggtt tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc
     5701 actgcttttg cttagcagtg ccttttttag gt
//
LOCUS       DACCXM010000328         5588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.guided.153, whole genome shotgun sequence.
ACCESSION   DACCXM010000328 DACCXM010000000
VERSION     DACCXM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.guided.153"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            107..571
                     /locus_tag="IVY61_003071"
     CDS             107..571
                     /locus_tag="IVY61_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7972089.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            555..1184
                     /locus_tag="IVY61_003072"
     CDS             555..1184
                     /locus_tag="IVY61_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7972090.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1538..3016
                     /gene="msr"
                     /locus_tag="IVY61_003073"
     CDS             1538..3016
                     /gene="msr"
                     /locus_tag="IVY61_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7972091.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3063..3509
                     /locus_tag="IVY61_003074"
     CDS             3063..3509
                     /locus_tag="IVY61_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7972092.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3708..4223)
                     /locus_tag="IVY61_003075"
     CDS             complement(3708..4223)
                     /locus_tag="IVY61_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7972093.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4340..5542
                     /locus_tag="IVY61_003076"
     CDS             4340..5542
                     /locus_tag="IVY61_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7972094.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1740 a          932 c         1197 g         1719 t
ORIGIN      
        1 agagcgtttt ttttattaca cagctgattc tttgactttt ttcttttttt attctattat
       61 tgacaagtca atcgttgacg agtcaattat tagaaaggag gaaattatgt ctttagaaga
      121 gattagtcac cttttatatc aaatcaaatt agccgatcaa aaaatcgtcc agatctttga
      181 agaaaatatc ggtataagtc ttactagata cgagatcatg atgagactga aagaagaaaa
      241 tccattgcta caaagcagtt tgcaagaaaa gttgcagatt gatcaaggag cagttactcg
      301 tcatctgaag gtattggaag caaatggcta tgtttcgcgt cagagaaatc ctcagaacaa
      361 ccgggaagtg tatgtagcgc taacagaaaa agcccagaag aaaatcaagc attgtgaaat
      421 ggaccataaa gatacaggtt ccgtgctatc cccggctttc agcgatgtag aaatgaaaca
      481 atttctaaca cttttagata aattgaatac ccaactttgc aatataacaa cagaaacgaa
      541 agaggttaat aataatggaa caaaaaaatg atttttcaac aattatgact ggtagaagat
      601 ccgttcgcgt atatgatgaa acatacaaaa tcccacacga agaaatgctg gaaatgatca
      661 aagaagcgac gactgcacct tcttctgtca atatgaaacc atggcgcttt gtggtcgctg
      721 aaagcgagga agcaaaagaa atcttgcgtc ctttgatccg tttcaatacg agacaaaatg
      781 atacttcttc tgcaatggtc atgatttttg gcgatatgca atgttacgaa tatggcgaag
      841 aaatctacaa ccaagcttac gaatcaggga agatgccaaa agaagtcaga gatcaacaat
      901 tagctgcgat cattccttat tacaagagtt tttcaagaga agaaatgaac gatgtagtca
      961 aagtcgattc tagcttagcg gctatgcaat tcatgttagt tgcgcgtgat catggctatg
     1021 aaacaaatcc aattggtgga ttcgaagcag atcagttggc agaggcattt ggtttggata
     1081 aagaccgtta tgttccagtt attattttat caataggaaa agcggtagaa gaaggttacg
     1141 agtctgttcg tttagcacca gaaaagatca ctatgttcga ataaaaggtt ttcagttact
     1201 attcagtagc tattgctttt tttgaatcgt gtgtggtaac ctaataaaaa aggaggtcgg
     1261 aaaaatgact gcatcgatga aattacgttt cgaacttttg aataacaact aaataaattc
     1321 aaaagacatg tataaacaac aatttcatgg atggagtatg tccatttttt cgaacgacag
     1381 tagaataggt gaaagctatg acagatttgg tgatttagtc atagttattt cgttcagagt
     1441 aggaaaagat gtgatgcagt ttttttgcaa acagattttt ctgctctttt ttagtatcga
     1501 taattgttgt tttacatgaa ataggagtgt aaaacatatg gaaaatttag cagtaaatat
     1561 aacaaatcta caagtcagct ttggcaacca gctagaacta tctattgatt ctcttcgtgt
     1621 ctatcagcaa gatcgtatag ggatcatagg agaaaatggt gtcggtaaat cgactttgct
     1681 caaactaata gccggtgaac tttttcccga tcatgggaaa atccaaacag agatcacctt
     1741 caactacctg cctcaattaa cctatctcgc tgaagcaaag gacctaaatt tggaattagc
     1801 cagtcatttc cagttaagac tggaagaaac ttcagagcgg aaatggagcg gaggggaaga
     1861 acgaaagatc gagttgatac gtcttctttc ttcttatgaa caagggatgc ttctagacga
     1921 gccaacaacc catctagata gaaaaagtat tgatcgactg attgaagagc ttcgttatta
     1981 ttatggtacg ctggtttttg ttagtcatga tcgctatttt ctagatgagc tagcatcgaa
     2041 aatctgggaa gtaaaagacg gagaaatccg agagttttcg gggaattata gtgcctatct
     2101 cactcaaaag gaattggaga aaaagactca gctacgagaa gcagagtcga tcatgaaaga
     2161 gaaaaaacga ttggaaaaat cgatccaaga aaagaaaaaa caagcggaaa agttggaaaa
     2221 agtgtccagt aaaaagaaaa agcaacaaat cagactggat cggttgtctt cctctaaaca
     2281 aaaagacagt gtacaaaaag ccatccaaaa gaatgcgaaa acattagaga gaagactcca
     2341 aaaaatagga gaaacaacca agccgcagca gatgaaacaa atccgttttc cagtaccaaa
     2401 atctcttgaa ctccacagcc gctatcccat catgggacaa aatgtccaat tggaaagaag
     2461 tgggagaaca ttactggtaa atggtgattt tcagttttct ttaggtaaaa aaatcgcgat
     2521 tgtcggtgaa aatggttcag gtaagacaac cttattagaa catatccgca aacaaggaga
     2581 aggaatcctt ctctctccga aagtaagctt tcaagtatat cagcaaaagg gttatcaaat
     2641 gacatctgaa gaatccatca ttcgttttgt catgagacaa acagagtttt cagaatcgct
     2701 tgtccgtagt ttgctgaatc acttagggtt tgctcaggaa actttgacga aaccgttatg
     2761 tacattaagt gggggagaag cgacccgtct gacgattgct ttgcttttta ctaagccaag
     2821 taatgtgttg ctgttagatg aaccgactaa ttttattgat atggcaacga tcgaagcttt
     2881 agagaagcta atgcaaatat atccgggaac gattttgttt acttctcatg attcttactt
     2941 tgtcgagcgt acggctgatg aagtttatga aataaaaggg cagaaaataa aaaaagtact
     3001 tacgagaaat ttttaaatgt gctacgatga ttttcgtgac aaatttaaag gagtggtaca
     3061 gattgcctac aaataaaaaa gaaggaataa tttttacaac aatgatgtgt tttttgatgg
     3121 tcttggggat gagcgcgtat aatttatggc tgcatcaaga tcttcattta tcaagtttgt
     3181 tgattggtct agtgcctgga ttcgtggtgg ctttcatttt ggatgtcttc atcatcggtg
     3241 tgctggcgaa aaaaattgcc tttaaactac cagtaaatcc agaaagcaag gtccaaatga
     3301 tcttaactat ttcatgcttg atggttttag gaatggtgac atgtatgtct ttattcggtg
     3361 tgatcatgga acaaggaata cctgatcaat tattctctgc ttatatgaat gcttggaaaa
     3421 tgaatgtgat tgccgcttta ccgttgcagt tgatcatcgt tggcccgctt tcaagaagtg
     3481 tgctaaaagc aattcaaagt aatgaatgat ggaacactgt tatttgaaaa aaggccttgg
     3541 catgcttttt gtcatagtct cttgaatgca gttctagaaa aatagtgatc cgaacgatag
     3601 gttttggcct gtcgttcgga tcgctttttc ttttccaact gtattgctct tttattgaag
     3661 ataatttgct cttcaataga tatttctaga aaggttctgt ttatccctta ctcaaaaaac
     3721 tgctgcattt gttctcttac ttgctgacac cattggagtt cctgctgaca aagcataatt
     3781 ctgcgctgga tcacaacata acgtccaaat tcttgtttct tgtctagcga gaggtcattc
     3841 aatttttttt gatttttttg catgattttg ttcagctgtt gttctctagt gaatagcagt
     3901 ccagtagctg ttgttttatc gagcgtcgaa aaagcataga tttttgctag aaattcatct
     3961 ttttgcgtag gagcgggagt ttcctctttg atccattcct ctacaagagc acgtccttca
     4021 tcagtcaagt ggtagatctt tttttgagag tgctcgtctt tgtccaaaac ggtcacataa
     4081 ccctgctggt ctaacttggc aagcgttgta tagatctgac tgtgatgggc ttcccaaaaa
     4141 attgtgatca gctgcttcaa ttcataccca gaacgtggtt tggataaaag cacgcataat
     4201 aatatatagg ctaaagtatt cataaaaatc ctacctctcg tcttattttc tttattatat
     4261 cacagtaaag tttccacgtt ttacatgtgc atattgacat gtaaaataaa tcatgtatga
     4321 tgctaataga gagaaaggga tggaaatatt ggagaatcaa atagatatac aagaacaaac
     4381 aaatgaagaa acaaagggga aaagttcagc gctagttgta gcagctacct gtatgatcgc
     4441 ttttatggga gtgggtctag tagacccaat cttaaaaacg attgctgcac agttaaacgc
     4501 tacgccagct gaaacgaccc tgttatttac tagttacatg ttagtcactg gtgtggtgat
     4561 gctatttaca ggtttcttat ctagtcggat cggtttgaaa aaaacattga tgacaggatt
     4621 gtttatcatc gttgtattcg ctggtcttgg cggattatct gggaatatcg gaatgctgat
     4681 tgggttacgt gccggctggg gtgtcgggaa tgctttattt gtctctactg ctttagcaac
     4741 gatcgtcagt attttagttg gtaacacaga aaaagcaatc atgatgtatg aagcagcact
     4801 tggctgcgga atggctgttg gccctctagt cggtggggtc ttgggcagtg gttcatggcg
     4861 ttatccattt tttggggtag catttttgat gttttgcggt tttatagccc tagcggtact
     4921 tttacctgaa actgagaaac caaagagaaa agtaggcctt tgggaaggtg tcaaagcgtt
     4981 aagcaatcat aaacttcgtt cagttggact aatcgcattg ttatataatt tcggattctt
     5041 tactttactt gcgtatgcgc catttttatt gattggctat tctgaattag aagtcggttt
     5101 tgtatttttt ggctggggtg tgcttctggc tattgcttcg attttcgttg caccggctct
     5161 tgagcggaag tttacgactt atcggacaat tttagcagca tttgtcttat tcattatttg
     5221 tttgctgtta ttaggagctg gaacttctat gccagtcctt gtaccgatca gcattgtgtt
     5281 agcaggattt ttccaaggaa ttatcaatac actgttgacg acgattgcta tggaaatacc
     5341 aggtctgcaa cgaaacgttg cttcgtccag ctatagtttt gtccgctttt tcggtggagc
     5401 tttagcacca tttattgcag gtgtgattgg tgaacatatt ggtgaaagct atgcgtttta
     5461 ttttgcaggt gccattgttc ttatcagtgt atgttgtatc atttatcacc gtaattattt
     5521 tgctacaaat gaagaagaat aagaaatcag aggttgtgaa aaagctgtcc tgcatcaagt
     5581 cataagaa
//
LOCUS       DACCXM010000329         4819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.guided.163, whole genome shotgun sequence.
ACCESSION   DACCXM010000329 DACCXM010000000
VERSION     DACCXM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.guided.163"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            191..997
                     /locus_tag="IVY61_003077"
     CDS             191..997
                     /locus_tag="IVY61_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP7972095.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1015..1842
                     /gene="vanW"
                     /locus_tag="IVY61_003078"
     CDS             1015..1842
                     /gene="vanW"
                     /locus_tag="IVY61_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7972096.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVFADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1839..2810
                     /locus_tag="IVY61_003079"
     CDS             1839..2810
                     /locus_tag="IVY61_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7972097.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2803..3831
                     /gene="vanB"
                     /locus_tag="IVY61_003080"
     CDS             2803..3831
                     /gene="vanB"
                     /locus_tag="IVY61_003080"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7972098.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3837..4445
                     /locus_tag="IVY61_003081"
     CDS             3837..4445
                     /locus_tag="IVY61_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7972099.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1360 a         1016 c         1276 g         1167 t
ORIGIN      
        1 agaatgattt tctatcagtc ctagaaatta tagagccgat tttgaccgac aggtataacc
       61 ctgccggtct ttttttgctg ctacaggaaa actacagact ggctacaggg aaagtacaga
      121 tgcgcttgcc ataataacaa tcgtaccagc cacaaatcgt agttttattg caaaggaggc
      181 attcgatcaa atggaaaaaa gcaactatca ttccaatgcg gatcatcaca aacggcatat
      241 gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc attatttcgt tcacagtctg
      301 cacgctgttt ttggggtgga gactggcttc cgtattggag gcaacacaga taccgcccat
      361 ccctgcaact catacaggca gcagcactga cgtagtggag aatttggagg aaaacgctct
      421 tgccaccgcc aaagaacagg gagatgaaca ggaatggagc ctgattttag tgaacaggca
      481 gaaccccatc cccgcacagt acgatgtgga gcttgagcaa ctatcaaatg gtgagcggat
      541 agatattcgg atttctccct atcttcaaga tttgtttgat gccgcaagaa ctgatggagt
      601 ttacccgatt gtcgcatccg gataccgaac aacagaaaaa cagcaagaaa ttatggatga
      661 aaaaattgcc gaatataagg cgaaaggcta cacctctgca caggctaaag cggaagcaga
      721 aacttgggtg gccgtgccgg gaacgagcga gcatcagctt ggtcttgctg tggatatcaa
      781 tgcggacgga attcattcaa caggcaacga ggtttataga tggctggatg aaaacagcta
      841 tcgttttggt tttattcgtc gctacccacc agacaagaca gagataaccg gtgtgagcaa
      901 cgagccgtgg cattaccgat atgttggcat cgaagctgcc acagagatgt acaaccaagg
      961 ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa aaggatgtta tgttatggac
     1021 agaaaaagat tgacacagcg tttcccgttc ctgcttccaa tgagacgggc gcagagaaaa
     1081 atgtgctttt atgcgggaat gagatttgac ggctgtcgct atgcgcagac gataggggaa
     1141 aaatcgcttt cccatttgct ctttgaaacg gattgcgcat tatacaacca caataccgga
     1201 tttgacatga tataccagga aaacaaggtg ttcaacttaa agctggcagc aaagacctta
     1261 aacggcttat tgataagacc gggggaaacc ttttctttct ggtggctggt acgccatgcg
     1321 gacaaagata ccccctataa agacggcctt acggtgacca atggtaaact caccaccatg
     1381 tcgggcggcg gtatgtgcca gatgagcaat ttactatttt ggatgttcct gcatacgcca
     1441 ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt ttccagagcc aaacagtgac
     1501 gagattaaag gggtggatgc aaccatttca gagggctgga ttgatttaaa agtgcgaaac
     1561 gataccgact gcacctacca aatatgggtg accttagatg atgagaaaat catcggtcag
     1621 gtgttcgccg acaaagagcc ccaagcatta tacaaaatta caaatggcag tatccagtat
     1681 gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta aacggatgca agttgcctta
     1741 ggtaccgggg aaataataga ttgcaagctg ctttatacaa acaaatgcaa aatctgttat
     1801 cccctcccgg aaagtgtgga tattcaggag gagaaccaat gagaaaaagt gtgggcatta
     1861 ctgtttttgg atgcgaacag gatgaggcaa atgctttccg cgctttatcg ccggattttc
     1921 atattatccc tacgctgatt agcgatgcga tatcggcaga caacgcaaaa ttggccgctg
     1981 gcaatcaatg cgttagcgta agccataagt ccgaggtttc cgaggcgaca attcttgcgc
     2041 tgagaaaggt cggggtaaaa tacatttcta cccgcagcat cggctgcgat cacattgata
     2101 cgactgccgc cgagagaatg ggaatctcgg ttggcacggt tgcgtattcg ccggacagcg
     2161 ttgcggatta tgctttgatg ctgatgctga tggccatacg gggtgcaaaa cccaccatgc
     2221 acgccgtggc gcaacaagat ttcagattgg atcgtatccg ggggaaagaa ctgggggata
     2281 tgactgtggg agttattgga accggccata tcgggcaagc ggtcgtcaaa aggctgcggg
     2341 gatttggatg ccatgtgctg gcctatgata acagccgaaa aatggatgca gattatgtcc
     2401 agcttgatga gcttctaaaa aacagcgata ttgttacgct ccatgtgccg ctttgtgcgg
     2461 atacccgcca tctgatcggt cagaagcaaa ttggagagat gaagcaaggc gcatttttaa
     2521 tcaacaccgg gcgcggagcg cttgtcgata ccggggcgct ggtggaggcg ttggaaagcg
     2581 gaaagctggg tggtgcggca ctggatgtgt tggagggcga ggatcagttt gtttataccg
     2641 attgctcgca gaaagtgctt gaccatccct ttttgtcgca gcttttaagg atgccgaatg
     2701 tgatcatcac accccatacg gcgtactaca ctgagcgtgt gctgcaggat actacagaaa
     2761 aaacaatcag gaattgtctc aattttgaaa ggagtttaca gcatgaatag aataaaagtc
     2821 gcaatcatct tcggcggttg ctcggaggaa catgatgtgt cggtaaaagc cgcaatagaa
     2881 attgctgcga acattgatac ggaaaaattc gatccgcact acatcggaat tacaaaaaac
     2941 ggtgtatgga agctatgcaa gaagccatgt acggaatggg aagccgacag tctccccgcc
     3001 atactctccc cggataggaa aacgcatggg ctgcttgtca tgaaagaaag cgaatacgaa
     3061 acacggcgta ttgatgtggc tttcccggtt ttgcatggca aatgcgggga ggatggtgcg
     3121 atacaggggc tgtttgtatt gtctggtatc ccctatgtgg gctgtgatat tcaaagctcc
     3181 gcagcttgca tggacaaatc actggcctac attcttacaa aaaatgcggg catcgccgtt
     3241 cccgaatttc aaatgattga taaaggtgac aagccggagg cgggtgcgct tacctaccct
     3301 gtctttgtga agccggcacg gtcaggttcg tcctttggcg taaccaaagt aaacggtacg
     3361 gaagaactta acgctgcgat agaagcggca ggacaatatg atggaaaaat cttaattgag
     3421 caagcgattt cgggctgtga ggtcgggtgt gcggtcatgg ggaacgagga tgatttgatt
     3481 gtcggcgaag tggatcaaat ccggctgagc cacggtatct tccgcatcca tcaggaaaac
     3541 gagccggaaa aaggctcaga aaatgcgatg attacagttc ccgcagacat tccggtcgag
     3601 gaacgaaatc gggtgcagga aacggcaaag aaagtatatc gggtgcttgg atgcagaggg
     3661 cttgcccgtg ttgatctttt tttgcaggag gatggcggca tcgttctaaa tgaggtcaat
     3721 accctgcccg gttttacatc gtacagccgc tacccacgta tgatggccgc cgcaggaatc
     3781 acgcttcctg cactgattga cagcctgatt acattggcgt taaagaggtg ataagtatgg
     3841 aaaatggttt tttgttttta gatgaaatgt tgcatggcgt ccgttgggat gccaagtacg
     3901 ccacatggga taacttcaca ggaaaaccgg tggatgggta tgaggtgaat cgcatcatcg
     3961 gcacaaaggc cgtggcgttt gctctgcgcg aggcacaaat ccatgcggct gcgcttggct
     4021 atggcttgct tttatgggat ggatatcggc caagaactgc ggtggactgc ttcctgcgtt
     4081 gggcagcgca accggaggac aatctcacaa aagaaaaatt ttaccccaat atagagcgag
     4141 ccgagttgat tacaaagggt tatgtggcct cacaatccag ccatagccgt ggaagcgcaa
     4201 ttgatcttac gctctaccac ctggatacag gggaacttgt ttcaatggga agtaacttcg
     4261 attttatgga cgaacggtcg catcatacag caaaagggat aggggatgca gaggcacaaa
     4321 atcgaagatg cttgcgtaaa atcatggaaa gcagcggatt tcagtcttat cgctttgaat
     4381 ggtggcacta taagttgatt gatgagccat accccgatac ctattttaat tttgccgttt
     4441 cataatgaaa gtattggttt tgtatcagat tttggatttg ataaaatcta tgctggtcat
     4501 ggacaatctt ttactggaaa gtggttaaag catcttttgt aatacattgc aaattatagt
     4561 attcaatctg cccagcggca gaaaagaaaa aaaccgctgt tgggcagacc tttcccacgc
     4621 ccattttgta ttttgctggc ggcggttttg agaaatcaag gccgccgttc ttttctggca
     4681 gcaaaaggtg tggcggccca taggaggatg ccgctatgct gttttgctat tgccatgctc
     4741 taatccactt ccaccactca aaaaaagcct tgcccctcca tgaactgaac cccaaaagtt
     4801 gaactattta atggactgt
//
LOCUS       DACCXM010000330         2772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13701
            SAMN11027181-rid9893953.guided.205, whole genome shotgun sequence.
ACCESSION   DACCXM010000330 DACCXM010000000
VERSION     DACCXM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027181
            Sequence Read Archive: SRR10694471
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743224.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743224.1
            Genome Coverage       :: 224x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 05:13:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 27 of 65
            Pseudo Genes (incomplete)         :: 32 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 7 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027181-rid9893953.guided.205"
                     /isolate="UW13701"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            111..194
                     /locus_tag="IVY61_003082"
     CDS             111..194
                     /locus_tag="IVY61_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP7972100.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="IVY61_003083"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="IVY61_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7972101.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..2182)
                     /locus_tag="IVY61_003084"
     CDS             complement(1319..2182)
                     /locus_tag="IVY61_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7972102.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2184..2456)
                     /locus_tag="IVY61_003085"
     CDS             complement(2184..2456)
                     /locus_tag="IVY61_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7972103.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2473..2682)
                     /locus_tag="IVY61_003086"
     CDS             complement(2473..2682)
                     /locus_tag="IVY61_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7972104.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          488 c          443 g          990 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt tgtttaaagg
     1981 tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac
     2041 tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgcta
     2101 gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat tgcttttcgg
     2161 tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat ttgttttgaa
     2221 tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa tggttgttga
     2281 aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc tagtaaacga
     2341 gtatttttag tatctagttc atgattcaaa acataattta atacccgatt ataaacactt
     2401 gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac tgccatttca
     2461 ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg
     2521 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg
     2581 caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg cgctgattgg
     2641 cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc acttctttct
     2701 atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga tttgtgatta
     2761 atcacttgtg at
//
